LOCUS       DAVKZB010000001        72230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKZB010000001 DAVKZB010000000
VERSION     DAVKZB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.001"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            147..935
                     /gene="codY"
                     /locus_tag="VXA80_000001"
     CDS             147..935
                     /gene="codY"
                     /locus_tag="VXA80_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV0352573.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            935..1510
                     /locus_tag="VXA80_000002"
     CDS             935..1510
                     /locus_tag="VXA80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0352574.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1592..1677
                     /locus_tag="VXA80_000003"
     tRNA            1592..1677
                     /locus_tag="VXA80_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Leu,seq:aag)
     gene            1960..3090
                     /gene="ugpC"
                     /locus_tag="VXA80_000004"
     CDS             1960..3090
                     /gene="ugpC"
                     /locus_tag="VXA80_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV0352575.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3237..3383)
                     /locus_tag="VXA80_000005"
     CDS             complement(3237..3383)
                     /locus_tag="VXA80_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352576.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3591..4349
                     /locus_tag="VXA80_000006"
     CDS             3591..4349
                     /locus_tag="VXA80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0352577.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4437..4949
                     /locus_tag="VXA80_000007"
     CDS             4437..4949
                     /locus_tag="VXA80_000007"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV0352578.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5131..6075
                     /locus_tag="VXA80_000008"
     CDS             5131..6075
                     /locus_tag="VXA80_000008"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV0352579.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            6084..6761
                     /locus_tag="VXA80_000009"
     CDS             6084..6761
                     /locus_tag="VXA80_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV0352580.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLFQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6859..7617
                     /locus_tag="VXA80_000010"
     CDS             6859..7617
                     /locus_tag="VXA80_000010"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV0352581.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7655..9160
                     /gene="lytC"
                     /locus_tag="VXA80_000011"
     CDS             7655..9160
                     /gene="lytC"
                     /locus_tag="VXA80_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV0352582.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9656..10174
                     /locus_tag="VXA80_000012"
     CDS             9656..10174
                     /locus_tag="VXA80_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV0352583.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFADAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10304..10810
                     /locus_tag="VXA80_000013"
     CDS             10304..10810
                     /locus_tag="VXA80_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV0352584.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10810..10980
                     /locus_tag="VXA80_000014"
     CDS             10810..10980
                     /locus_tag="VXA80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352585.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            11012..12244
                     /gene="clpX"
                     /locus_tag="VXA80_000015"
     CDS             11012..12244
                     /gene="clpX"
                     /locus_tag="VXA80_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV0352586.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12253..12840
                     /locus_tag="VXA80_000016"
     CDS             12253..12840
                     /locus_tag="VXA80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV0352587.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGIDEAWDAILEKL"
     gene            12851..13231
                     /locus_tag="VXA80_000017"
     CDS             12851..13231
                     /locus_tag="VXA80_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV0352588.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13283..14173
                     /gene="rapZ"
                     /locus_tag="VXA80_000018"
     CDS             13283..14173
                     /gene="rapZ"
                     /locus_tag="VXA80_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV0352589.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14170..15147
                     /locus_tag="VXA80_000019"
     CDS             14170..15147
                     /locus_tag="VXA80_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV0352590.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15144..16055
                     /gene="whiA"
                     /locus_tag="VXA80_000020"
     CDS             15144..16055
                     /gene="whiA"
                     /locus_tag="VXA80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV0352591.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16089..17057)
                     /locus_tag="VXA80_000021"
     CDS             complement(16089..17057)
                     /locus_tag="VXA80_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0352592.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17193..18050
                     /locus_tag="VXA80_000022"
     CDS             17193..18050
                     /locus_tag="VXA80_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV0352593.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            18037..18816
                     /locus_tag="VXA80_000023"
     CDS             18037..18816
                     /locus_tag="VXA80_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV0352594.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18840..20192
                     /locus_tag="VXA80_000024"
     CDS             18840..20192
                     /locus_tag="VXA80_000024"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV0352595.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20266..20640
                     /locus_tag="VXA80_000025"
     CDS             20266..20640
                     /locus_tag="VXA80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV0352596.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20642..21490
                     /locus_tag="VXA80_000026"
     CDS             20642..21490
                     /locus_tag="VXA80_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0352597.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21756..22523
                     /locus_tag="VXA80_000027"
     CDS             21756..22523
                     /locus_tag="VXA80_000027"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV0352598.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLERLL"
     gene            22535..23719
                     /locus_tag="VXA80_000028"
     CDS             22535..23719
                     /locus_tag="VXA80_000028"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV0352599.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23716..25587
                     /locus_tag="VXA80_000029"
     CDS             23716..25587
                     /locus_tag="VXA80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0352600.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25730..26914)
                     /gene="mntE"
                     /locus_tag="VXA80_000030"
     CDS             complement(25730..26914)
                     /gene="mntE"
                     /locus_tag="VXA80_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV0352601.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27218..29914
                     /locus_tag="VXA80_000031"
     CDS             27218..29914
                     /locus_tag="VXA80_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV0352602.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKITNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29927..30718
                     /gene="yghU"
                     /locus_tag="VXA80_000032"
     CDS             29927..30718
                     /gene="yghU"
                     /locus_tag="VXA80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV0352603.1"
                     /translation="MSAYQLPTVWQDEASDQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30834..31244
                     /locus_tag="VXA80_000033"
     CDS             30834..31244
                     /locus_tag="VXA80_000033"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0352604.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31256..32851
                     /locus_tag="VXA80_000034"
     CDS             31256..32851
                     /locus_tag="VXA80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352605.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            32918..33082
                     /locus_tag="VXA80_000035"
     CDS             32918..33082
                     /locus_tag="VXA80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV0352606.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LVAVWLGWPK"
     gene            33161..33889
                     /gene="yaaA"
                     /locus_tag="VXA80_000036"
     CDS             33161..33889
                     /gene="yaaA"
                     /locus_tag="VXA80_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV0352607.1"
                     /translation="MKILIPTAKEMNTDLPSIEATPLKPESQAVLDALALYSASQLES
                     FYKVSAEKATEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(33940..35424)
                     /locus_tag="VXA80_000037"
     CDS             complement(33940..35424)
                     /locus_tag="VXA80_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0352608.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            35640..42569
                     /locus_tag="VXA80_000038"
     CDS             35640..42569
                     /locus_tag="VXA80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0352609.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIIGLELRSNSNKGTVSETPVAKKADTTEAKEEAKPAAR
                     TTDRSAVDSSVLATNAPAKVETTSAPKSTNEILKPGLNLSDARQNPAIRKEDVDRGYS
                     GFRTAGSGFRAAGSGFRAAGPENKPILNPNNTIAFSDISQGSHSFRGIGHSRGGREIH
                     YDVTTVRRGDNIEFTINYSAPGNLREFVNNNFILDKGDGFGNPSNATITSSNPRVREQ
                     SKSISQGANYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGG
                     GGAATSNDPYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQT
                     RLVREGEKGQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPR
                     VDQNKVGDTNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKG
                     EKGDRGERGLTGAQGAKGEKGDRGERGLTGAQGAKGEKGDRGERGLTGAQGAKGEKGD
                     RGERGLTGAKGEKGDRGERGLTGAQGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQ
                     GEKGDRGERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTST
                     VVRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEK
                     GDKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFD
                     GASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNG
                     KDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTT
                     LTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDG
                     DTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGE
                     KGDQGERGLTGSKGEKGDQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPA
                     GPQGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGR
                     NGTNGQSPTITATRSVQNGKNGVLVTITPVEGRPQTTFVEDGQKGADGKTPTVTITEG
                     QNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTE
                     TGDVYVKNNGNWDKEGNIKGPKGDKGAQGAKGEKGAQGERGLTGAQGAKGADGAVGRD
                     GRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERG
                     EDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKN
                     GNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETK
                     VYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQP
                     TTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNG
                     KDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNS
                     KDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTE
                     NPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAI
                     IKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNG
                     DGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGR
                     EPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGR
                     EYKSIIKDGKDGKDSVSPTVTVKNNNYGTHVVTITNPDGSKTEMVIKDGKDGKSPKVS
                     VEDNGDGSHTITIINSDGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPS
                     NDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQ
                     PVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRR
                     KEA"
     gene            complement(42806..43354)
                     /locus_tag="VXA80_000039"
     CDS             complement(42806..43354)
                     /locus_tag="VXA80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0352610.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            43675..44163
                     /locus_tag="VXA80_000040"
     CDS             43675..44163
                     /locus_tag="VXA80_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV0352611.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            44160..45347
                     /locus_tag="VXA80_000041"
     CDS             44160..45347
                     /locus_tag="VXA80_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0352612.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYSFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            45340..45705
                     /locus_tag="VXA80_000042"
     CDS             45340..45705
                     /locus_tag="VXA80_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0352613.1"
                     /translation="MIDHFEIKVKDLQILEGFYRSFLVPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            45721..47067
                     /gene="asnS"
                     /locus_tag="VXA80_000043"
     CDS             45721..47067
                     /gene="asnS"
                     /locus_tag="VXA80_000043"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV0352614.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            47220..47510
                     /locus_tag="VXA80_000044"
     CDS             47220..47510
                     /locus_tag="VXA80_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV0352615.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            47522..47992
                     /gene="ssbA"
                     /locus_tag="VXA80_000045"
     CDS             47522..47992
                     /gene="ssbA"
                     /locus_tag="VXA80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV0352616.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            48024..48263
                     /locus_tag="VXA80_000046"
     CDS             48024..48263
                     /locus_tag="VXA80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV0352617.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            48558..49958
                     /locus_tag="VXA80_000047"
     CDS             48558..49958
                     /locus_tag="VXA80_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV0352618.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            49960..50319
                     /locus_tag="VXA80_000048"
     CDS             49960..50319
                     /locus_tag="VXA80_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV0352619.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            50373..51122
                     /locus_tag="VXA80_000049"
     CDS             50373..51122
                     /locus_tag="VXA80_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV0352620.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            51112..51411
                     /locus_tag="VXA80_000050"
     CDS             51112..51411
                     /locus_tag="VXA80_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV0352621.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            51482..52417
                     /locus_tag="VXA80_000051"
     CDS             51482..52417
                     /locus_tag="VXA80_000051"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV0352622.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            52504..53016
                     /locus_tag="VXA80_000052"
     CDS             52504..53016
                     /locus_tag="VXA80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV0352623.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            53041..53649
                     /locus_tag="VXA80_000053"
     CDS             53041..53649
                     /locus_tag="VXA80_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV0352624.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            53750..53947
                     /locus_tag="VXA80_000054"
     CDS             53750..53947
                     /locus_tag="VXA80_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0352625.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(53990..55435)
                     /locus_tag="VXA80_000055"
     CDS             complement(53990..55435)
                     /locus_tag="VXA80_000055"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV0352626.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            55541..57163
                     /locus_tag="VXA80_000056"
     CDS             55541..57163
                     /locus_tag="VXA80_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0352627.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            57366..59324
                     /locus_tag="VXA80_000057"
     CDS             57366..59324
                     /locus_tag="VXA80_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0352628.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            59496..61178
                     /locus_tag="VXA80_000058"
     CDS             59496..61178
                     /locus_tag="VXA80_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352629.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKYFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTIYSSSKGSSVALENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENR
                     PVLKDITCSIFKGQKIAFVGPSGSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKE
                     WRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDT
                     EVGERGRLLSGGRSQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEK
                     TVVIIAHRLSTVKDVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            61336..62430
                     /locus_tag="VXA80_000059"
     CDS             61336..62430
                     /locus_tag="VXA80_000059"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0352630.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            62438..63604
                     /locus_tag="VXA80_000060"
     CDS             62438..63604
                     /locus_tag="VXA80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0352631.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            63697..66795
                     /locus_tag="VXA80_000061"
     CDS             63697..66795
                     /locus_tag="VXA80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0352632.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIVQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            66843..67460
                     /locus_tag="VXA80_000062"
     CDS             66843..67460
                     /locus_tag="VXA80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0352633.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            67471..68805
                     /locus_tag="VXA80_000063"
     CDS             67471..68805
                     /locus_tag="VXA80_000063"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV0352634.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDT
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            68815..69279
                     /locus_tag="VXA80_000064"
     CDS             68815..69279
                     /locus_tag="VXA80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0352635.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            69276..69770
                     /locus_tag="VXA80_000065"
     CDS             69276..69770
                     /locus_tag="VXA80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0352636.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            69851..71506
                     /gene="mltG"
                     /locus_tag="VXA80_000066"
     CDS             69851..71506
                     /gene="mltG"
                     /locus_tag="VXA80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV0352637.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            71577..72059
                     /gene="greA"
                     /locus_tag="VXA80_000067"
     CDS             71577..72059
                     /gene="greA"
                     /locus_tag="VXA80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV0352638.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        22118 a        13306 c        16407 g        20399 t
ORIGIN      
        1 caatagaaaa ggaacaattt aattaaaaat attctggttt ttaattaaat tgtaagtcaa
       61 tgcaagtcaa taaggaattt tcagataatt attgaaaaag agacgttttt atgttataat
      121 gatagcgatt aaattcgagg tgaaaaatgg cacatttatt agaaaaaact agaaaaatta
      181 catcaatttt gaaacgctca gaggagcagt tgcaggatga gctcccttac aacgctatta
      241 cgcgtcagtt agcggatatt attcattgca atgcctgcat tatcaatagt aagggacgtc
      301 tgcttggcta ttttatgcgt tataaaacaa atacagatcg cgtagagcaa ttcttccaaa
      361 ctaagatttt cccagatgac tacgttcaag gggctaatat gatttacgaa acagaagcaa
      421 acttgcctgt tgagcatgat atgagtattt tccctgttga gagtagagat gattttccag
      481 atggcttgac gactattgca ccgattcatg tatcggggat tcgccttggt tctttgatta
      541 tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt tcttgttgag attgccagta
      601 ccgttgttgg gattcagctt cttaacttcc aacgtgaaga agatgagaaa aatattcgtc
      661 gtcgtactgc tgtcaccatg gcggtcaata ccctttctta ctccgaactc cgtgctgttt
      721 cagcaatttt aggggaatta aatggaaatg aagggaagtt gactgcgtca gtgattgcag
      781 atcgtatcgg aatcactcgc tctgtgattg tcaatgctct tcgtaaactt gagtctgcgg
      841 ggattattga aagtcgctca cttggaatga agggaaccta tcttaaggtc ttgatttcag
      901 atatttttga agaagtgaag aaaagagatt actaatgaca aaggctttaa tttcgattga
      961 ttatacagaa gattttgttg ctgatagtgg gaagctgaca gcaggtgctc cagctcaggc
     1021 gatttcggat gccattagca aggtaactcg attagctttt gaacgaggag attatatctt
     1081 ctttactatt gatgctcatg aagaaaacga ttgtttccat ccggaaagta agttatttcc
     1141 tcctcataat ctgattggga cgagtggacg gaatttatat ggagatttgg ggatctttta
     1201 tcaagagcat ggttcagaca gtcgtgtctt ttggatggat aaacgccatt actcagcttt
     1261 ttcagggact gacctagata ttcgtttgag agagcgtaga gtgtctaccg ttatcttaac
     1321 aggtgtcttg acggatatct gtgtcctaca tacagctata gatgcctata atctaggata
     1381 tgacatcgag attgttaaac cagctgttgc ttccatctgg cctgaaaatc atcaatttgc
     1441 cctaggtcat ttcaaaaata cactcggagc taagttagta gatgaaaatc taaatgaact
     1501 ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa aaaagtgttg acaagcatcc
     1561 caaaagttga tatactagta aagtaatcga cgcggggatg gcggaattgg cagacgcgca
     1621 ggactaagga tcctgtgacc gctttaggtc gtgagggttc aagtccctct ctccgcatag
     1681 gaaaagagtt agctaaagct agctcttttg tatttctcta aaaaattaga atccagtcaa
     1741 atgtagtgga gaatcagcga aaatatggga aaactgatcc ttgtttctgc ttttgtcata
     1801 gaagaataat aaagattatc ttcttcaaat tctccgatat tctctaaagt tttgtgcaag
     1861 ttgcacagaa cttttttatt tttttggtca tcttgccata gaaatataaa gcgttttcat
     1921 ctataatata attatcaaaa aacaaaagga gttcacctca tggtagaatt gaatcttaaa
     1981 aatatttaca aaaaatatcc aaacagcgaa cactattcag ttgaagattt caacttgaac
     2041 atcaaagata aagaatttat cgttttcgta ggaccttcag gatgtggtaa atcaactaca
     2101 ctccgtatga ttgctggtct tgaagacatt acagaaggta ctgcatctat cgatggcgtg
     2161 gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca tggtattcca aaactacgct
     2221 ctttacccac acatgactgt ttatgacaac atggctttcg gtttgaaatt gcgtaaatac
     2281 agcaaagaag acattaacaa acgtgttcaa gaagcagctg aaatacttgg attgaaagaa
     2341 ttcttggaac gtaaaccagc tgacctttca ggtggtcaac gtcaacgtgt tgccatgggg
     2401 cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg aacctttgtc aaacttggat
     2461 gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa ttcaccgtcg tatcggagct
     2521 acaactatct atgtaactca cgaccaaaca gaagcgatga cacttgcaga ccgtatcgtt
     2581 attatgtcag ctactaagaa ccctgctggt acaggtacta tcggacgtgt agaacaaatc
     2641 ggtactcctc aagaagttta caaaaatcca gttaacaaat tcgttgcagg attcatcgga
     2701 agcccagcta tgaacttcat caacgtgaaa ttggttggta gcgaaattgt ttctgacggt
     2761 ttccgtttga aagtgccaga aggagcattg aaagttcttc gtgaaaaagg ctacgaagga
     2821 aaagaattga tctttggtat ccgtccagaa gacgtgaatg cagaacctgc tttccttgaa
     2881 acattcccag actgtgttgt aaaagcgact atctctgtat cagaactgct tggttcagaa
     2941 tctcaccttt actgccaagt tggtaaagac gagtttgttg caaaagttga tgctcgtgac
     3001 tacttgcaaa caggtgcaac agttgagctt ggatttgact tgaacaaagc acacttcttc
     3061 gatgtagaaa ctgaaaaaac aatctactaa aataaataaa attcaaagca ctacaagaaa
     3121 agatatctct ttatcaattg tagtggagag atatcagtta atctagggag agaaacaaaa
     3181 tgcttctctc ctttttgcta gggaagtcat attatgcatc tatattgtga tgctctttaa
     3241 tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtacgt atgattactg
     3301 atttcgtcag ttttatctgc aacctcaaag atgtactttg agcagcttac ggctagtttc
     3361 ctagtttgct ctttgatttc cattgagtat tatttgtggg taccatctac aagtgaagct
     3421 atatgcgtaa actacatgag caattgaatt cgaactagag aggtaataat aaatttatgc
     3481 tatagttatg gtgacttgta tgcttttgat tctagtttat caaataatag attagaattg
     3541 tcagataata tcattttgtg ttataatgaa gaaaaaacag aggtgttcaa atgtcagaag
     3601 caggtcataa gtttttagca aaattgggga aaaaacgctt acgtccaggt ggaaagcgtg
     3661 ccacagattg gttaattgca gaaggaggat tttcaaaaga aaagagaata ctagaggttg
     3721 cgtgtaatag gggaactaca gcaattgagt tggcacagcg ttttggttgc aagataactg
     3781 ctgttgatat ggatgctcaa gctttagaag tggctaaaaa atctgctgga acggcaggtg
     3841 ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa acttccttat caagatgcta
     3901 gttttgatat tgttataaat gaagctatgc tgactatgca agccgatcaa gctaagaaaa
     3961 aatgtgtaat ggaatatcta agggtattaa aacctggagg tcttctcttg acacatgatg
     4021 tgcttcttaa ggaagctaaa gagtctatca gacaggaatt atcacaagca attcatgtaa
     4081 atgtaggtcc tttaactcaa gatggttggg aacaggtgat gatagaatca ggttattgtg
     4141 atgtgaaagc attgactggt gaaatgacat taatgaaatt atcgggtatg atttatgacg
     4201 aaggttggct aggaactttg aaaatttgtg taaatgcttg taaaaaggag aatagaaagc
     4261 agtttttaac tatgtataaa atgtttgcta agaataaaca gaaattgggc tttattgcga
     4321 tggctagtta taaatcgtca aaacgttaga taattattga agttaacttt tccttttttc
     4381 tttcttaaaa aatatgctat aatagagagt aaaaaacttt gaaagaaaga aaaaagatga
     4441 atttaaaaga ttacattgca acaattgaaa attatccaaa ggaaggcatt accttccgtg
     4501 atattagtcc tttgatggct gatggaaatg cttatagcta cgctgttcgt gaaatcgttc
     4561 agtatgctac tgacaagaaa gtcgacatga tcgtgggacc tgaagctcgt ggatttatcg
     4621 tgggttgtcc agttgccttt gagttgggaa ttggttttgc gcctgttcgt aagccaggta
     4681 aattgccacg cgaagttatt tctgctgact atgaaaaaga gtacggtgtc gataccttga
     4741 ctatgcacgc ggatgccatt aagccaggtc aacgtgttct tattgtagat gaccttttgg
     4801 cgacaggtgg aactgttaag gcaactatcg agatgattga aaaacttggt ggtgttatgg
     4861 caggttgtgc cttccttgtt gaattggatg aattgaacgg ccgtgaaaaa attggtgact
     4921 acgactacaa agttcttatg cattattaat gaaaacagtc cctagggctg ttttctctac
     4981 actaggatat aaaaatagac tataactagt tagagaaaaa ctataattga aaactatatc
     5041 ttcttgcagt ataataaaag gactaagtgt ttgagatttg tcttcaaaca tatgcaatta
     5101 ttcctgaaag agtacagtta ggagagggtt atgccgattc gaattgataa aaaattgcca
     5161 gctgttgaga ttttacggac agagaatatc tttgtcatgg atgatcaacg tgctgcccac
     5221 caagatatcc gtcctttgaa gattttaatt ttaaatctca tgccacagaa aatggtcaca
     5281 gagacccagt tgttgcgcca cttggctaat acacccctac aactggatat tgattttctc
     5341 tatatggaga gccaccgttc taaaacaact cgttcagagc acatggagac cttctataaa
     5401 acttttcctg aagtcaagga tgagtatttt gatgggatga tcatcacggg tgctccagtt
     5461 gagcatttac catttgagga agtggactat tgggaggaat ttagacagat gcttgagtgg
     5521 tctaagactc atgtctattc gacccttcat atctgttggg gggctcaggc tgggctttat
     5581 ctgcgctatg gtgtagaaaa ataccagatg gacagtaagc tatcaggtat ttatcctcag
     5641 gacaccctaa aagagggtca ccttctattt agaggctttg atgatagcta tgtatcccct
     5701 cattcacggc acacggagat ttctaaggaa gaggtcttaa acaagaccaa tctcgagatt
     5761 ttatcagaag gacctcaggt tggggtttct attttggcca gtcgtgattt acgagaaatt
     5821 tatagttttg gtcatttgga gtatgaccgt gataccttgg caaaagagta ttttcgagat
     5881 cgtgatgcag gtttcgaccc acatattcca gaaaattact ttaaagatga tgatgttaat
     5941 caggtacctt gtctttgttg gtcttcatct gcagccctct ttttcagtaa ttgggtaaac
     6001 catgcggtct atcaggagac gccttttgat tggagaaaga tagaagatga tgcatctgca
     6061 tatgggtatt tataagagga attatgacat atttagacgc ttttaaatca ggtaccttgg
     6121 ttttaccgag tgccctgctc ttgcatttta aggaactctt tccttctagc gacgattttc
     6181 tggtttggca atttttctat ttgcaaaata cgacaggctt agaagaaatg tcgccaagcc
     6241 agattgctga aaggattggc aaggaaattt cggatgtcaa ccagtccatt tctaatctga
     6301 cggaaagggg actgttccag tatcgtacta tcgaattaaa tggcgaaatt gaattgctct
     6361 ttgatgctag tttggccttg gaacgtttgg atgacctgtt tggagcagtt cattcaagtt
     6421 cagaccagct aacacctcaa aaccagctca aggatttggt ggaaaccttc cagcaggagt
     6481 tgggacgatt gttgacgcct tttgagattg aggatttgac caagacacta aaggaagatg
     6541 gaaccagtgc tgacttgatt aaggaggctc ttcgtgaagc tgttttgaat ggaaaaccaa
     6601 actggaagta cattcaggcg attttgagaa actggcgcca tgaaggaatc aagagtgtgg
     6661 ctcaaattga ggccaagaga gcagaaagag aagcaagcaa tcctcagttg acacaggtat
     6721 ctgcagattt cagaaatgcc atggatctct ggaaggatta atccatgcaa gtaggcttga
     6781 aatccgagta agatttgcaa gctgtgtata attgtgatag aataaataga aaataaattg
     6841 aaaaaagagg tatgtgaaat gtcacgtaaa ccatttatcg ctggtaactg gaaaatgaac
     6901 aaaaatccag aagaagctaa agcattcgtt gaagcagttg catcaaaact tccttcatca
     6961 gatcttgttg aagcaggtat cgctgctcca gctcttgatt tgacaactgt tcttgctgtt
     7021 gcaaaaggct caaaccttaa agttgctgct caaaactgct actttgaaaa tgcaggtgct
     7081 ttcactggtg aaactagccc acaagttttg aaagaaatcg gtactgacta cgttgttatc
     7141 ggtcactcag aacgccgtga ctacttccat gaaactgacg aagatatcaa caaaaaagca
     7201 aaagcaatct ttgcgaacgg tatgcttcca atcatctgtt gtggtgaatc acttgaaact
     7261 tacgaagctg gtaaagctgc tgaattcgta ggtgctcaag tatctgctgc attggctgga
     7321 ttgactgctg aacaagttgc tgcctcagtt atcgcttatg agccaatctg ggctatcggt
     7381 actggtaaat cagcttcaca agacgatgca caaaaaatgt gtaaagttgt tcgtgacgtt
     7441 gtagctgctg actttggtca agaagtcgca gacaaagttc gtgttcaata cggtggttct
     7501 gttaaacctg aaaatgttgc ttcatacatg gcttgcccag acgttgacgg tgcccttgta
     7561 ggtggtgcgt cacttgaagc tgaaagcttc ttagctttgc ttgactttgt aaaataatca
     7621 gtaagtagca aaagctaggt ggaacagcat tcagatgtct gttacatttt ttataggaga
     7681 gcaaagattg aaaacaaaaa ttggattagc aagtatctgt ttactaggct tggcaactag
     7741 tcatgtcgct gcaaatgaaa ctgaagtagc aaaaacttcg caggatacaa cgacagcttc
     7801 aagtagttca gagcaaaatc agtcttctaa taaaacgcaa acgagcgcag aagtacagac
     7861 taatgctgct gcccactggg atggggatta ttatgtaaag gatgatggtt ctaaagctca
     7921 aagtgaatgg atttttgaca actactataa ggcttggttt tatattaatt cagatggtcg
     7981 ttactcgcag aatgaatggc atggaaatta ctacctgaaa tcaggtggat atatggccca
     8041 aaacgagtgg atctatgaca gtaattacaa gagttggttt tatctcaagt cagatggggc
     8101 ttatgctcat caagaatggc aattgattgg aaataagtgg tactacttca agaagtgggg
     8161 ttacatggct aaaagccaat ggcaaggaag ttatttcttg aatggtcaag gagctatgat
     8221 gcaaaatgaa tggctctatg atccagccta ttctgcttat ttttatctaa aatccgatgg
     8281 aacttatgct aaccaagagt ggcaaaaagt gggcggcaaa tggtactatt tcaagaagtg
     8341 gggctatatg gctcggaatg agtggcaagg caactactat ttgactggaa gtggtgctat
     8401 ggcgactgac gaagtgatta tggatggtgc tcgctatatc tttgcggcct ctggtgagct
     8461 caaagaaaag aaagatttga atgtcggctg ggttcacaga gatggtaaac gctatttctt
     8521 taataataga gaagaacaag tgggaaccga acatgctaag aaaatcattg atattagtga
     8581 gcacaatggt cgtatcaatg attggaaaaa ggttattgat gagaacgagg tggatggtgt
     8641 cattgttcgt ctaggttata gcggtaaaga agacaaggaa ttggcgcata acattaagga
     8701 gttaaaccgt ctgggaattc cttatggtgt ctatctctat acctatgctg aaaatgagac
     8761 cgatgctgag aatgacgcta aacagaccat tgaacttata aagaaataca atatgaacct
     8821 gtcttaccct atctattatg atgttgagaa ctgggaatat gtaaataaga gcaagagagc
     8881 tccaagtgat acagacactt gggttaaaat catcaacaag tacatggaca cgatgaagca
     8941 ggcgggttat caaaatgtgt atgtctatag ctatcgtagt ttattacaga cgcgtttaaa
     9001 acacccagat attttaaaac atgtaaactg ggtagcggcc tatacgaatg ctttagaatg
     9061 ggaaaaccct tattattcag gagaaaaagg ttggcaatat acctcttctg aatacatgaa
     9121 aggaatccaa ggtcgcgtag atgtcagcgt ttggtattaa gcgatgattt gaaagaggga
     9181 tgtgatagta gcaccctctt tttctttgtt ttatgatagt tcatcctcga gtaaattcaa
     9241 gatcttgctc ggaaatgaag cttatatagt agattgaata tagacaaata ccttgtgatt
     9301 ggtaaaacat tttagaaatt catttacctt tcctaatcga cttggtttca tcttatttca
     9361 atctattata gtattgggga atttcttcaa accacatcag cttggtcagt tctacctgcg
     9421 acctcaaaac ttgtgctttg gtcaagctgg gtttagtttc ctagtttgct gatggatttc
     9481 cattgactat aagcatccaa ccctcttttt gtcttctaaa gaattcttaa attatcagtc
     9541 tattgcaact tttctcatat aagttctttg tcttgctatt ggttttcctt agtagtatac
     9601 taaggtagta atcattaaga agtggttaca aaaaataatg aatgaggtaa agaaaatggt
     9661 agaattgaaa aaagaagcag taaaagacgt aacatcattg acaaaagcag cgccagtagc
     9721 attggcaaaa acaaaggaag tcttgaacca agctgttgct gatttgtatg tagctcacgt
     9781 tgctttgcac caagtgcact ggtatatgca tggtcgtggt ttccttgtat ggcatccaaa
     9841 aatggatgag tacatggaag ctcttgacgg tcaattggat gaaatcagcg aacgcttgat
     9901 tacactcggt ggaagcccat tctctacatt gacagagttc cttcaaaata gtgaaatcga
     9961 agaagaagct ggtgaatacc gtaatgttga agaaagcttg gaacgtgttc ttgttatcta
    10021 ccgttacttg tcagaacttt tccaaaaagg tttggatgtc actgatgaag aaggtgacga
    10081 tgtgacaaac ggtatctttg cagacgctaa aactgaaaca gataaaacaa tttggatgct
    10141 tgcagccgaa cttggacaag cacctggttt gtaagaaata aggaaacata gataaatctg
    10201 taggaaattt cttacagatt ttttctattt tgtaagctat tccaaaccag tctttttttg
    10261 agttttttga taaaatagta ctatcagtga aaaggatgga agcatgacta agaaaatcgt
    10321 agctatttgg gcccaggatg aagagggtgt gattggtaag gaaaatcgtc tgccttggca
    10381 tttgccagca gaattgcagc actttaaaga aacaactctg aatcatgcta tcttgatggg
    10441 gcgtgtgacc tttgatggga tggggcgtcg cttgcttcca aaacgggaaa ccctgatttt
    10501 gacgcgtaat ccggaagaaa agatagatgg ggttgctact tttcaggacg tccagtctgt
    10561 tcttgactgg tatcaggatc aagaaaagaa tctctacatt atcggtggga agcaaatttt
    10621 tcaggctttt gaaccttacc ttgatgaagt gattgtcact cacattcatg ctcgggtgga
    10681 aggagatacc tatttccctg aagagcttga cttgtctctt tttgagacag tttcaagcaa
    10741 attttacgcc aaagatgaga agaatcctta tgattttacc atccaatacc gcaagagaaa
    10801 ggaagtctaa tggaacggag tttatttggt ctattcacgg cttttctgtg tttcatctgc
    10861 tttctggcag gagcacaagc ttttcgtaaa aagcgttacg ggctttctat cctcctttgg
    10921 ttaaatgcct ttaccaatct ggtcaatagt attcatgctt tttacatgac cttattttag
    10981 atagaatgac agtatagaat ggaaggaaat catgtctaca aatagaaaaa atgatatgat
    11041 ggtttattgc tcattttgtg gcaaaaacca agaagaagta caaaaaataa ttgctggcaa
    11101 caatgctttt atttgtaatg aatgcgtgga gttagctcag gaaatcattc gagaagaatt
    11161 ggttgaggaa gtcttggcag acttgtctga ggtgccaaaa ccaattgaac tcctccatat
    11221 cttgaaccac tatgtaattg gtcaagatcg tgccaagcgt gccttggcag tggcggttta
    11281 taaccactac aaacgcatca atttccacga tacacgcgaa gagtcagaag atgtggattt
    11341 gcagaagtca aacattttga tgattggccc aactggttca gggaaaactt tccttgccca
    11401 gaccttggct aagagcttga atgtaccttt tgctattgcg gatgcgacag ctctgacgga
    11461 ggctggttat gtgggtgagg atgtggaaaa tatcctcctc aaactcttgc aggttgctga
    11521 ctttaacatc gaacgtgcag agcgtggcat tatctatgtg gatgaaattg acaagattgc
    11581 caagaagagt gagaatgtgt ctatcacacg tgatgtttct ggtgaagggg tgcaacaagc
    11641 ccttctcaag attatcgagg gaactgttgc tagcgtgccg cctcaaggtg gacgcaaaca
    11701 tccacaacaa gagatgattc aagtggatac aaaaaatatc ctcttcatcg tgggtggtgc
    11761 ttttgatggt attgaagaaa ttgtcaaaca acgtctgggt gaaaaagtca tcggatttgg
    11821 tcaaaacaat aaggcgattg acgaaaacag ctcatacatg caagaaatca tcgctgaaga
    11881 cattcaaaaa tttggtatta tccctgagtt gattggacgc ttgcctgttt ttgcggctct
    11941 tgagcaattg accgttgatg acttggttcg catcttgaaa gagccaagaa atgccttggt
    12001 gaaacaatac caaaccttgc tttcttatga tgatgttgag ttggaatttg acgacgaagc
    12061 ccttcaagag attgctaata aagcaatcga acggaagaca ggggcgcgtg gacttcgctc
    12121 cattatcgaa gaaaccatgc tagatgttat gtttgaggtg ccgagtcagg aaaatgtgaa
    12181 attggttcgc atcactaaag aaactgttga tggaacggat aaaccgatcc tagaaacagc
    12241 ctagaggtga ctatggaact taatacacac aatgctgaaa tcttgctcag tgcagctaat
    12301 aagtcccact atccgcagga tgaactgcca gagattgccc tagcagggcg ttcaaatgtt
    12361 ggtaaatcca gctttatcaa cactatgttg aaccgtaaga atctcgcccg tacatcagga
    12421 aaacctggta aaacccagct cctgaacttt tttaacattg atgacaagat gcgctttgtg
    12481 gatgtgcctg gttatggcta tgctcgtgtt tctaaaaagg aacgtgaaaa gtgggggtgc
    12541 atgattgagg agtacttaac gactcgggaa aatctccgtg cggttgtcag tctagttgac
    12601 cttcgtcatg acccgtcagc agatgatgtg cagatgtacg aatttctcaa gtattatgag
    12661 attccagtca tcattgtggc gaccaaggcg gacaagattc ctcgtggtaa atggaacaag
    12721 catgaatcag caatcaaaaa gaaattaaac tttgacccaa gtgacgattt catcctcttt
    12781 tcatctgtca gcaaggcagg gatagatgag gcttgggatg caatcttaga aaaattgtga
    12841 ggaaaagaaa atggcaaaaa caattcatac agataaggcc ccaaaggcta tcgggcccta
    12901 tgttcaagga aaaatcgttg gcaacctttt gtttgctagc ggtcaagttc ccctatcccc
    12961 tgaaactggg gaaattgtag gagagaatat ccaagaacag acagagcaag tcttgaaaaa
    13021 catcggtgct attttggcag aagcaggaac agactttgac catgttgtca aaacaacttg
    13081 tttcttgagc gatatgaacg actttgttcc ttttaatgag gtttaccaaa cggccttcaa
    13141 agaggaattc ccagctcgtt cagctgtgga ggtagctcgt cttcctcgtg atgtaaaagt
    13201 cgaaattgaa gtcatcgcag agattggata agctagttga agtttggtgt tgccaaactt
    13261 cttttgatat aaggagaaaa agatgacaaa gaaacaactt cacttggtga ttgtgacagg
    13321 gatgagtggc gcagggaaaa ctgtagccat tcagtccttc gaggatctag gttatttcac
    13381 cattgataat atgccgccag ctctcttgcc taagtttttg cagctggttg aaattaagga
    13441 agacaatcct aagttggcct tggtagtgga tatgcgtagc cgttctttct tttcagagat
    13501 tcaagctgtt ttggatgagt tggaaaatca agatggtttg gatttcaaaa tcctcttttt
    13561 ggatgcggct gataaggaat tggtcgctcg ttacaaggaa accagacgga gtcacccact
    13621 agcagcagac ggtcgtattt tagatggaat caagttggaa cgtgaactct tggcaccttt
    13681 gaaaaatatg agccaaaatg tggtggatac gactgaactc actccacgtg agctgcgcaa
    13741 aacccttgca gagcagtttt cagaccaaga acaagcccag tctttccgta tcgaagtcat
    13801 gtctttcgga tttaagtatg gaatcccgat tgatgcggac ttggtctttg atgtccgttt
    13861 cttgccaaat ccctattatt taccagaact gagaaaccaa acgggtgtgg atgaacctgt
    13921 ttatgattat gtcatgaacc atcctgagtc agaagacttt tatcaacatt tattggcctt
    13981 gattgagccg attctgccaa gttaccaaaa ggaaggtaag tccgttttga ccattgctat
    14041 gggatgtacg ggtggacaac accgtagtgt ggcatttgct aaacgcttgg cgcaggactt
    14101 atccaagaat tggtctgtta atgaagggca tcgcgacaaa gaccgcagaa aggaaacggt
    14161 aaaccgttca tgagaaaacc aaagataacg gtgattggtg gagggactgg aattcccgtc
    14221 attctaaaaa gtctgcggga aaaagatgtg gaaatcgcag ctatcgtgac ggtggcagat
    14281 gatggtggtt cttcaggtga actccgaaaa aatatgcaac agttgacacc gccaggtgat
    14341 cttcgtaatg tccttgtggc catgtcggat atgcctaagt tttatgagaa ggtctttcag
    14401 tatcggttct ctgaggatgc cggagccttt gctggccatc cattgggaaa tctcatcatt
    14461 gctggcttgt cagaaatgca gggttcaacc tataatgcca tgcagttatt gagcaaattt
    14521 ttccatacaa cagggaaaat ttatccttct agtgaccatc ctttgaccct tcatgcagtc
    14581 tttcaggatg ggacagaagt ggctggagag agtcatattg tagaccatcg aggcataatt
    14641 gacaatgtct atgtgaccaa tgccctaaac gatgatacgc ctctggccag ccgtcgagta
    14701 gtgcagacca tccttgaaag tgacatgatt gtcctagggc caggttccct ctttacctct
    14761 attttgccca atatcgtgat taaggaaatt gggcgggctc ttttggaaac taaggcagaa
    14821 attgcctatg tctgcaatat catgacccaa cgtggggaga cggaacactt tacagatagc
    14881 gaccacgtgg aagtcttgca tcgtcacctt ggtcgccctt ttatcgacac tgtcttggtg
    14941 aatattgaaa aagtgcctca ggaatacatg aattccaacc gttttgatga atacttggtg
    15001 caagtggaac acgattttgt aggtctttgt aagcaagttt cgcgcgtgat ttcatctaac
    15061 ttccttcgtc tggaaaatgg cggtgccttc cacgatggag atttgattgt ggacgagttg
    15121 atgcgcatta tacaggtgaa aaaatgagtt tcacagtagc agtaaaagaa gaaatcctgg
    15181 gccaacacca cctgagccgg catgaattat ctgccattat caaaatgtct ggtagcatcg
    15241 gtctctcgac ttcgggcttg actttgtcag ttgtgacaga aaatgccaaa ctggcccgtc
    15301 acctctatga gtcctttctc catttctatg aaatcaaatc tgaaattcgt cactaccaac
    15361 ggagcaatct tcgcaagaat cgcgtctata ccgtttttac agatgaaaag gtgcaggatt
    15421 tgttaagtga tttgcacttg gcagactctt tctttggcct ggaaacaggt attgatgagg
    15481 cgattttatc ggacgaggaa gcaggtcgtg cctatctctg tggcgctttc ttggcaaatg
    15541 ggagcattcg tgaccctgag tcaggcaagt accagttgga aatcagttct gtttatctgg
    15601 accacgcgca aggaattgcc tcccttctcc aacagttttt actggatgcc aaggtgcttg
    15661 agcgcaagaa gggggctgtg acctatctcc agcgagcaga agacattatg gacttcttga
    15721 tagtaatcgg agccatgcag gcacgtgatg attttgagcg ggttaagatt ttgcgagaaa
    15781 cccgtaacga cctcaatcgg gctaataatg ccgagacagc taatatcgct cggacagttt
    15841 ctgccagcat gaagaccatt aacaatatca gtaaaatcaa agatatcatg ggcttagaaa
    15901 atttgccagt agatttgcag gaagtggcgc aactgaggat tcagcaccca gactactcta
    15961 tccagcagtt agcagatagt ctcagcaccc ctctgaccaa aagtggtgtc aatcacagac
    16021 tcagaaaaat caataagata gcggatgaat tataaaacca cgaatcctat gtgactcgtg
    16081 gttctttttt ataaactagt agagtgtttt ggttgtactt tttgttcagg gtcaatgtag
    16141 ttgatagcgt tgttgacagc agttggagct tctccgaggc ctgtcgcaat cagatcaatt
    16201 tttccgtcat agtagcagca gtcaccgata gcatagatac ctgcttggct ggattcctgt
    16261 ttgctgttga cgataatctt gtgacggttg aggtcgagcc cccagttttt aaggttaccg
    16321 acagaagatt tgaaaccata gttgacaaag aggtggtcta ggtcaatggt ttcagtttca
    16381 tcagatttga cttttgtgat ttcaagttta tcaagtgttt ttccatttcc aaggagttgg
    16441 ctaggggcga atggtgtctt gatggttaca gatgattctt gcaaggcttg aacactgtgt
    16501 tccaaggcac ggaaattatc tctgcggtga acaagggtag ttggtgcgat tttttcaaaa
    16561 gccaaagccc aatccacagc cgagtctccc ccaccaagaa tcgtcacttt cttaccagcg
    16621 tattgctgaa tgttagaaac gtggtagtgg atattttcat agccctcaac actttcaagt
    16681 tccagcggac gtggtttgaa ggcaccgcca cccatagcga tgataactgt tttagtcagg
    16741 tgacttcctt tagaagttgt gatggcaaat tcttcttctt gtttgtcaat ctcaagaacc
    16801 gtttcattga gatgaatagg ggtatcaaat ccatttagct gttcaatcaa gcggttagtc
    16861 aactcttctc cagtcaggtt tgggaagcct ggtacgtcta ggatttcctt ttcagggtag
    16921 agaatagcag gttgtccacc tagctgggga agagagtcga tgatttgaac cttggcttgg
    16981 cgtaggtggg cataaaaggc tgcaaaaagc ccgacaggac caccacccac aatggtaata
    17041 tcatagagtt gagacatagt ttctcctttg ttttttctag tcagtttatt ttatcatatt
    17101 tttgttcaat gtaaaatgga ctaggatagg gaaaaaaatg ccagaaaatg gtataatagg
    17161 acgaaagtgt tttgataggg aggatatcag ggatgaattt tcaacaatta tccaatctgc
    17221 aatattggac cagtttgttt gcaagtccat ggacgatagc tatcaatctg attgatattt
    17281 tgattgttgc ttatatttta taccatttta caaaagctat tgcaggaacc aagattatga
    17341 ttttggtacg tggagttttg gtgtttattt tagctcaaat ccttgcaaat atgattggtt
    17401 tgactacgat ttcttggtta atcaatcaaa ttattactta tggggttatt gcggcggttg
    17461 ttatcttctc tccagagatt cggactggtt tggaacgttt gggaagagcg acagattttt
    17521 tttccaatgc ccctattagt gctgaggaac agatgattcg tgcctttgtt aagtctgttg
    17581 aatacatgag tcctcgtaaa atcggggcct tggttgctat tcagcgtgta cgtaccttgc
    17641 aggagtatat ttcgacagga attcccttgg atgctaagat ttctgcagaa cttctcatta
    17701 acatttttat tcccaacact cccctacatg atggtgcggt gattatcaaa gaagaacgta
    17761 tcgctgtgac gtctgcctat ctgcccttga caaaaaacac agggatttcc aaggaatttg
    17821 ggaccagaca ccgggcggct atcggtttat cagaagtctc agatgccttg acttttgtcg
    17881 tatcagagga aacgggagga atctcgataa cctataatgg aaggtttaag cacaacctaa
    17941 cacttgatga atttgaaaca gaattacgtg aaatcttact tccaaaagag gaagtgggtc
    18001 ttagttttaa agaacgattg ctaggaggat ggaaacatga aaaaaaatag tttatatatc
    18061 atatcctcac tcttttttgc ttgtgtctta tttgtctatg ctacggcgac gaattttcaa
    18121 aacagtacca gtgctaggca ggtaaaaacg gaaacctata ctaatacagt aacaaatgtc
    18181 cctattgaca tacgctataa tagtgataag tattttatta gcggttttgc ttcagaagta
    18241 tcagtggtct tgactggtgc aaatcgccta tcgctagcta gtgaaatgca agaaagtaca
    18301 cgtaaattca aggttactgc tgacctaaca gatgccggtg ttggaacgat tgaagttcct
    18361 ttgagcattg aagatttacc caatgggctg accgctgtgg cgactccgca aaaaattaca
    18421 gtcaagattg gtaagaaggc tcagaaggat aaggtaaaga ttgtaccaga gattgaccct
    18481 agtcaaattg atagtcgggt acaaattgaa aatgtcatgg tgtcagataa agaagtgtcg
    18541 attacgagtg accaagagac attggataga attgataaga ttatcgctgt cttgccaact
    18601 agcgaacgca taacaggtaa ttacagtggt tcagtacctt tgcaggcaat cgaccgcaat
    18661 ggtgttgtct taccggcagt tatcactccg tttgatacaa taatgaaggt gactacaaaa
    18721 ccagtagcac caagttcaag cacatcaaat tcaagtacaa gcagttcatc ggagacatct
    18781 tcgtcaacga aagcaactag ttcaaaaacg aattaaaaat agaaaaggat tttataacaa
    18841 tgggtaaata ttttgggact gatggagtcc gtggagaagc taacctagaa ctaacaccag
    18901 aattagcctt taaactagga cgttttggag gctatgttct tagtcaacat gaaacggaag
    18961 cgccgaaagt ctttgtagga cgtgacacac gtatttcagg ggaaatgctg gaatcggcct
    19021 tggtggcagg tctcctttca gtagggattc acgtatacaa acttggtgtc cttgcaacac
    19081 cagcagtagc ttacttggtt gaaactgaag gagcaagtgc cggtgtcatg atttctgcta
    19141 gccacaaccc agcccttgat aacggaatca agttctttgg cggtgatggc ttcaaactag
    19201 atgatgaaaa agaagcagaa attgaagcct tgctagatgc tgaggaagac actcttcctc
    19261 gtccaagtgc agaaggctta ggaattttgg tagattatcc agaaggcttg cgtaagtatg
    19321 aaggatacct tgtttcaact ggaactcctc ttgatggaat gaaggttgcc ttggatacag
    19381 ctaatggagc agcttctacc agtgcccgtc aaatctttgc agaccttggt gcccaattga
    19441 cggttatcgg ggaaacacca gacggtctta acatcaacct taatgttggt tcaacacatc
    19501 cagaagccct tcaagaagtg gtcaaagaaa gtgggtcagc tattggtttg gcctttgatg
    19561 gagacagtga ccgcttgatt gctgttgatg agaatggtga catcgtcgat ggtgacaaga
    19621 ttatgtacat catcggaaaa tacctttctg aaaaaggaca attggctcaa aatacaattg
    19681 tgacaactgt tatgtctaac cttggtttcc acaaggcctt gaatcgcgaa ggtattaaca
    19741 aggcagttac tgcagttggt gaccgctacg ttgttgaaga aatgagaaaa tcaggctaca
    19801 accttggtgg tgaacagtct ggtcacgtta tcttgatgga ttacaatacc acaggtgatg
    19861 gtcaattatc agcagttcaa ttgactaaaa tcatgaagga aactggtaag agcttatcag
    19921 agttggcggc agaagtaacg atttatccac aaaaattagt taatatccga gtggaaaacg
    19981 tcatgaagga aaaggccatg gaagtgccag ctatcaaggc catcatcgag aagatggaag
    20041 aagaaatggc ggggaacggc cgtatccttg ttcgtccaag tggaacagag cccctcttgc
    20101 gtgttatggc agaagcgcct acaacagaag aagtggacta ctatgttgat accatcacag
    20161 atgtagttcg tgctgaaatt gggattgact aaatcctaga aaactagatg aaaataaaaa
    20221 attatggtac aatagcttat aatattgtag ccgatggaga aagacatgaa tcttaaacgt
    20281 gaacaagaat ttgttagtca gtatcatttt gatgcgcgta attttgaatg ggaaaatgaa
    20341 aatggagctc ctgaaaccaa ggtggatgtg aatttccaat tgctccaaca tgaccaagaa
    20401 aatcaagtga cttccttgat tgtcatcttg agctttatga ttgtatttga taagtttgtc
    20461 attagtggaa caatctcaca agtaaaccat atcgatggcc gtattgtcaa tgaaccaagt
    20521 gaattgaacc aagaagaggt ggaaaccttg gctcgcccat gtttgaacat gctcaatcgt
    20581 ttgacttacg aagtgactga aattgcatta gacttaccag gaattaattt ggagttctaa
    20641 tatgaaatta gctgttttca cagattcctc tgcttatctc agtgcagaga tcttgcaaag
    20701 agaagacttg tttgtcttgg atattcctgt caatattgat ggtgaggaat atgtcgaagg
    20761 catcaatctg tctgctgagg aattttacca aaaaatggct caggcttctg aattgcctaa
    20821 gaccagtcaa ccaagtattg ccaagttaga tgaaatctta acttcgctca aagaacaagg
    20881 ctatacacat gccttggggc ttttcctatc ttctggaatt tcaggttttt accaaaatat
    20941 ccagtatatg gtcgatgact atgagggctt aaccattgct ttcccagaca ctttgattac
    21001 aagtgctccc ctaggcatca tggttgaaag cgtctttaat tggcgtgatc agggcgatga
    21061 ttttgccagc attcaggata agctagccat tcaaatcagc cgtacgtcag cctttatcat
    21121 ggtagatgac ttggatcatt tggtaaaagg tggacgcctt tcaaatgggg ctgccatttt
    21181 gggcaatttg cttagcatta agccaatcct ttattttaac gatcaaggtg tgattgaagt
    21241 ttacgaaaaa gttcgtactg aaaagaaggc caccaaacgc ttaattgaaa ttatcaagga
    21301 aacaacggct tcaggtcaat accgggtcat tgtcattcat gggaatgctc ctgaaaaggc
    21361 tgaagaattg cgtcagcatt tgcttgattt tggcttgggt tcggatgttt cacttgctac
    21421 atttggtagt gtcattggaa cgcatctagg agcaggaagt attgctctgg gttatattcc
    21481 agtgatttag taggcacttc taggctagtt aatcttagca attgaacacg gactacctgc
    21541 ctcttgaaaa aagatgtctg tcttgccttt cgtggaaagt cagtgccatt ccctattttt
    21601 catgggtggc taagatcctt tgtatcttga taattgaaca cgcctggaac cctgtgtgaa
    21661 aaagatagtt cttccaagga gtagacactt cttgatcaga attcctattt tcactttgtg
    21721 ttcttacagg ctttgtatct tagacaggag tagagatgag tattcgagta attattgccg
    21781 gttttaaggg aaagatgggt caagctgctt gccagatggt cttgactgat tcagatttgg
    21841 acttggtagc agttttggat ccttttgagt ctgagtcaga atggcagggt attcctgttt
    21901 tcaaggataa ggctgattta gctggttttg aagcggatgt ctgggtagac tttactactc
    21961 cagctgttgc ctacgaaaat acacgttttg ctcttgaaaa tggctttgct ccagtagttg
    22021 gaacgactgg tttcacgagt gaagaaattg cagagctaaa agaattttct cgtgcccaag
    22081 acttgggtgg cctgattgcc cctaactttg ccttgggtgc tgtcttactc atgcaatttg
    22141 cgacgcaggc tgccaaatat ttcccaaatg tggagattat tgagctccat catgacaaga
    22201 aaaaggatgc tccgagtgga acagccatta aaacagctga gttgatggca gaggttcgag
    22261 agtcaattca gcaaggtgca gcagatgagg aagagctgat tgctggtgct cgtggtgctg
    22321 actttgatgg tatgcgcatc cactcagttc gtttgccagg cttggtagct catcaggaag
    22381 tcatctttgg caatcaggga gaagggttga ccctccgtca tgactcctat gatcgcatct
    22441 ccttcatgac aggagtcaat ttgggaatta aagaagttgt caagcgtcat gagcttgtct
    22501 atggattaga acgcttatta tgagattaac gcaaatgcct tctgaatttc agaaggcttt
    22561 accagtatta gaaaaaatta aagaagcagg ctttgaggct tattttgttg ggggctctgt
    22621 tcgagatgcc cttctccata gccctatcca cgatgtggat attgcgacgt cttcttatcc
    22681 agaagagacc aagcagattt ttccgcgaac agccgatatc ggaatcgagc atggaaccgt
    22741 cttggtctta gatggggatg aggagtatga ggtaacaacc tttcggacag aggatgtcta
    22801 tgtggactat cgcagaccca gtgcggtttc ctttgtgcgc tcgctagaag aagacctcaa
    22861 acgccgtgat ttcacagtca acgcctttgc cttggatgag acaggagaaa tcgttgactt
    22921 gttccatggt ttagaagatt tggaaaagca agtcttgcga gcagttggag tggctagtga
    22981 gcgtttcaac gaagatgctt tacggattat gcgtggtttc cgttttcagg ctagtcttgg
    23041 ttttgcactt gagccagaaa catttaaagc tatgaagacc ttgacgccgc ttttggagaa
    23101 aatttctgta gagcgtacct tcgtcgagtt tgataaactc ttgctggctc cattttggag
    23161 aaggggcttg gcttccatga ttgagagtca agcttatgac tatctccctg atatggcatc
    23221 tagccagaac aagctcaaca gactgtttga tttggagact gattttactt ttgaatcctc
    23281 tgaacaagcc tgggcggctt tactatgggc tttggagatt gaaaatgcgc agtcattttt
    23341 gaaatcttgg aagacctcac gccagtttgc caagcaagtt caggatttgc tgattatttt
    23401 ggctctgcgt gaaaatggag aattgagcaa gcgagattgt tatcgctttg acatagattt
    23461 gcttttacag gctgaaaatc ttcgtcaagc tcgaggaaaa gaagtcaacc cacaagccat
    23521 cacagaaaaa taccaaagct tgactatcca tgacaagaaa gagattcaga ttaatggcga
    23581 tattttgatc aaggaatatg gctatcagcc aggcccagac ttgggagaga ttttaacaga
    23641 gattgagttt gccattgtcg atggagaatt ggagaataat cgtgaagcca tccatgctta
    23701 cctgagggag aaaaaatgag tgattttatc gttgaaaaac taagtaaatc cgttggtgac
    23761 aagaccgttt ttagggatat ttcctttatt atccatgact tagacagaat tggtttaatc
    23821 ggtgtcaatg gaactggcaa gaccaccctt ttggacgtcc tttctggtgt ttctggattt
    23881 gatggggatg tcagtccttt ttcagctaaa aatgattacc agattggtta cttgactcag
    23941 gatcctgatt ttgatgatag aaagacagtt ttggatacgg ttctatctag tgaactcaag
    24001 gaaatccagc tcattcgtga gtatgaattg attatgctcg actatagtga ggacaagcag
    24061 gcgcgtttgg aacgtgtcat ggcagagatg gactctctcc aagcttggga aatcgagagt
    24121 caggtcaaga ccgttcttag caaattgggc attcaagact tatctactcc tgttggggaa
    24181 ttgtcaggtg gtctgagaag acgggtacag ttggcacaag tcttacttgg caaccacgac
    24241 ctcttgcttt tggatgagcc gaccaaccat ctggatattg cgattattga gtggctgacc
    24301 ctctttttga aaaattctaa gaagaccgtc ctttttatca ctcacgatcg ttatttctta
    24361 gacgctttgt caacacggat tttcgagttg gagcgagcag gcttgacaga atatcagggc
    24421 aattaccagg actatgttcg ccttaaggca gaacaggacg agcgcgatgc agctctactt
    24481 cataaaaaag aacaactcta caaacaagaa ctggcctgga tgcgcagaca accgcaggca
    24541 cgtgcgacca agcaacaagc tcgtatcaat cgtttccatg atctgaaaaa ggaagtttca
    24601 ggcagtagtg ctgagacaga cttgactatg aactttgaaa ccagtcggat tgggaagaaa
    24661 gtcatcgagt ttcaggatgt ttcctttgcc tatgaaaata agcccatttt gcaaaatttt
    24721 aatctcttag ttcaggctaa agaccgtatt ggaattgttg gggacaatgg tgttggaaaa
    24781 tcaaccctac ttaacctgat tgcaggaagt cttgagccga cagcaggaca agttgtgatt
    24841 ggggaaactg ttcgcatcgc ctatttctct caacaaattg agggtttgga tgaaagcaag
    24901 cgtgtgatca attacctgca ggaagtggca gaggaggtca agaccagtgg tggttctacg
    24961 acttccatcg ctgagttgct ggagcaattc ctcttcccac gttcgacgca tgggaccttg
    25021 attgagaaat tgtcaggggg tgagaaaaaa cgtctttatc tcctcaaact gcttttggaa
    25081 aaaccaaatg ttcttctttt agacgagcca accaatgacc tagatattgc aactttgaca
    25141 gtcttagaga atttcttgca aggttttgca ggtcccgttt taacagtcag tcacgaccgc
    25201 tatttcttgg ataaggtagc gaccaagatt ctcgcttttg aggatggcaa gattcgtcct
    25261 ttctttggtc attacaccga ctatcttgat gaaaaagctt ttgaaacaga tatggccaat
    25321 caagtgcaaa aggccgaaaa ggaaaaagtg gtcaaggttc gagaagacaa gaaacgcatg
    25381 acctaccaag aaaagcagga gtgggcaagt attgaaggtg atattgaaac cttggaaaaa
    25441 cgtatcgctg ctattgaaga ggaaatgcag gctaacggct ctgactttgg taagctggct
    25501 actctccaaa aagaattgga tgagaaaaat gaagcactcc ttgaaaaata cgaacgctat
    25561 gagtatctca gtgaatttga tagttaagac tattcgaaaa tcaaagtgca aactatgtcg
    25621 gcttcacatt gccgactcaa gtacagcctg tgaccggctt cctaatttgc actttgattt
    25681 ttattgagta taaaagaata gaaaaatccc gtctcatgaa aggatttctc tattcttttt
    25741 tggtttcttg atgaaagata ttttgccagg tttcgtggcg acgccagata ctggtatgga
    25801 tgataccatc taactcatag cagatgagtt tggttttttg actgatggaa gtaatttgaa
    25861 tgtccttgat agcagaattt aactcttttt tggtcttata agcctcttta tccatctgct
    25921 ctccatcttg gcgaatatag acaaaatcat cagtcaagag ttcttctagt tggtttcctt
    25981 ggtcaatcaa ttgttcacgc ataagcaatt ttttatagac attgtctaaa atttcatcct
    26041 cagggatagg tgctggttcg atatggacat cggtatcaaa gacgccaaaa cgctcctcca
    26101 gcatagactc gacctgatcc gcgatttcat ggctttcaaa aacagacaag tcaggattca
    26161 tctctagtgt aatatccagg tagatgttgc taccgtaggt gcgacctctt tgcgatttga
    26221 ccttgctgat tttgggaatt tccatgatag ccttttggta gtcctcgagc aggcggtcgt
    26281 caaagccatc tgaaagacta aaggaagact cgatgaagat atcataggca gtcttcaaga
    26341 taaagaaagt gatgatgata gcaaccagtt tatccacaat cggataatta aaactactag
    26401 ctaggatggc aatggcggtt ccaagtgagg taacagcgtc agaaagattg tccttagcag
    26461 ctgccttcag cgccttggag ttggatttct tactgaggcg agtattgtag agatagacca
    26521 caaacataat cgctgcagaa atgattccta gagttgcacc aagaggatca atgaccgttt
    26581 cttcccgact gagaatcttt tgaatggtat ctcttagaac atcgaaaccg acatagaaca
    26641 tgatgataga agtgatcaag cttgccaaat cttcaatctt ccaatgacca aaacggtggt
    26701 cacggtctgc aggctggcgc gccatccgaa tcccgattaa gagggccaca tttccaatga
    26761 tgtccgatac gttattaaaa ccatcggcca ccaaactgga tgaatgaagg agatgaccag
    26821 ctgctaattt ggctgcagac aagatcaaat aggtcgaaat actgataatg gctccacgct
    26881 cagctaactt gagatttgag atagattgct tcattcaact tcctttctag tcacatagca
    26941 ttctaccatt atactggttt tcaaggaaaa attcaaataa ggcagttttt actctttgaa
    27001 aactccccaa aatttggtat aatggtacta ctcaagggag tagctggcag aaacctgtga
    27061 tagtgtcgtc attccgaatt ttatgctgaa aagtatgctt tccggcccta tcttaaacag
    27121 cgagacttgt tatgattaac aggtctcttt ttgtttgtcg tgaaaagata tgacagaaag
    27181 agagtctgtt ttgcacacaa tgtcaaggag gagacacatg tcaaaagaac aaaaacgcca
    27241 agcgttttat actcagagcc ctgaagaggt cttgcaggct gtggatgcga ccgagcaagg
    27301 tttgtcatca agtgaggcgg aaaagcgcct tgccgaattt gggcacaatg aactcgaaga
    27361 aggcgagaaa cgatcaatct tggtcaaatt catcgagcaa tttaaggatt tgatgattat
    27421 catcctagtt gcggcagcaa tcttgtcagt cgtgacttct ggtggggaag atatcgcaga
    27481 tgccattatc atcctagctg tggttatcat caacgctgcc tttggtgttt accaagaagg
    27541 aaaagctgaa gaagctattg aagccctcaa atccatgtct agtccagttg cccgcgttct
    27601 tcgtgatgga cacatggcag agattgactc taaagaattg gtaccaggcg atatcgttgc
    27661 ccttgaagca ggtgacgtgg taccagcgga cctacgtttg atagaagcca actctcttaa
    27721 aattgaagaa gcagccttga caggtgaatc tgtaccagtc gaaaaagact tgtcagtcga
    27781 gcttgcgaca gatgctggta ttggtgaccg tgtcaacatg gccttccaaa actcaaacgt
    27841 aacctatggt cgtgggatgg gtgttgttgt caatacaggt atgtacactg aagttggtca
    27901 tattgctggt atgcttcaag atgcggatga gactgataca ccactcaaac aaaatttgaa
    27961 caacctttct aaggtcttga cctatgctat cttggtcatt gcccttgtta cttttgtagt
    28021 gggtgtcttc attcaaggga aaaatccact tggtgagttg ttgacttctg ttgcccttgc
    28081 cgttgcagcc attccagaag gacttcctgc tatcgttacc atcgttcttt ctcttggtac
    28141 tcaagttttg gccaaacgac attccatcgt tcgtaagttg ccagcagttg aaacacttgg
    28201 ttcaactgaa atcatcgctt ctgataagac tggtacgctg actatgaaca agatgacagt
    28261 cgaaaaagtc ttctacgatg cggttctaca tgactcagct gatgatattg aactaggtct
    28321 tgaaatgcca ctacttcgtt cagttgtctt ggccaatgat acgaaaatcg atgtggaagg
    28381 taacttgatt ggtgacccaa ccgaaacagc ctttatccaa tatgccttgg acaagggcta
    28441 tgatgtcaaa ggtttcttag agaaatatcc tcgtgtagct gaattgccat ttgactctga
    28501 ccgtaagctc atgtcaacag ttcacccatt gccagatggt cgtttccttg tagcagtcaa
    28561 gggtgcgcca gaccaactct taaaacgttg tcttcttcgt gataaggctg gggatattgc
    28621 tccgattgat gagaagatta caaatctcat tcatacaaac aactctgaaa tggctcatca
    28681 agccttgcgt gtccttgcag gtgcttataa gattattgac agcattccag aaaatctcac
    28741 ttctgaagag cttgaaaatg atttaatttt tactggtttg attgggatga ttgaccctga
    28801 acgtcctgaa gccgctgagg ctgttcgtgt ggctaaggaa gcgggaatcc gtccaattat
    28861 gattacaggt gaccaccaag acacagcgga agccattgcc aaacgtttgg gaatcattga
    28921 cgcaaacgat acagaaggtc acgttttaac tggtgctgaa ctcaatgaac tgtcagatga
    28981 agaatttgaa aaagttgttg gtcaatactc tgtttatgcc cgtgtgtctc cagaacacaa
    29041 ggttcgtatc gtcaaggctt ggcaaaaaca aggtaaagtc gttgccatga caggtgacgg
    29101 tgtcaatgac gcgccagctc tgaaaacagc cgatattggt atcggtatgg gaatcactgg
    29161 tacagaggtt tctaaggggg cttctgatat gattcttgca gatgataact ttgcgactat
    29221 tatcgtcgca gtggaagaag gacgtaaggt cttctcaaac attcaaaaga ctattcagta
    29281 cctactttct gctaatactg ctgaagtatt aaccatcttc ctatcaacct tgtttggttg
    29341 ggatgtcttg cagccggttc atcttttgtg gatcaacttg gtaacggata ccttcccagc
    29401 tatcgctctt ggtgttgaac ctgcggagcc tggtgtcatg aatcataaac cacgtggacg
    29461 caaggcaagc ttcttctcag gtggtgtttt gagttctatc atttatcaag gtgtactcca
    29521 agcagctctt gttatgagtg tttatggcct tgcgattgct tacccagttc atgtgggtga
    29581 caatcatgct attcatgcag atgccctaac gatggccttt gcaacccttg gtttgattca
    29641 gctcttccat gcctacaatg tcaagtctgt ctaccaatcc atcttgacag ttggcccatt
    29701 caagtctaag acctttaact ggtccatctt ggtatccttt atccttctca tggcaacaat
    29761 cgttgtagaa ccgcttgaag gaatcttcca cgtaaccaaa ctagacttgt cacaatgggg
    29821 aattgttatg gctggaagct tctcaatgat tatcatcgtc gaaatcgtta agtttattca
    29881 acgcaaactc ggttttgaca agaatgcgat ttaagagagg tttcggatgt cagcatatca
    29941 attaccgacc gtatggcagg atgaagctag tgatcaagga gcttttacgg ggctaaacag
    30001 accaacagca ggtgcccgtt tcgaacaaaa cttgccaaaa ggagaacaag cttttcagct
    30061 ttattcactg ggaacaccaa atggtgtgaa ggttactatc ttattggaag aattactaga
    30121 agctggtttt aaggaagcgg cttacgactt gtataagatt gctatcatgg atggggatca
    30181 attcggctca gactttgtga agctcaatcc aaattccaag attccagcct tattggacca
    30241 gtcaggtact gaaaacgtaa gagtctttga gtctgctcat attcttcttt accttgctga
    30301 gaaatttgga gcctttttac caagtaatcc tgtggaaaag gtagaagttt tgaattggct
    30361 attctggcaa gcaggtgcag caccttttct aggtggggga tttggacatt tcttcaatta
    30421 tgctcctgaa aaattggaat atcctattaa ccgttttacg atggaagtga aacgccagtt
    30481 ggatttattg gataaggaat tggctcagaa accttatatt gcaggcaatg actatacgat
    30541 tgcagatatt gctatctggt cttggtatgg acagttagtt caaggaaatc tttaccaagg
    30601 ttctgcaaaa ttcttggatg cctcaagtta tcaaaatcta gtaaaatggg cagaaaaaat
    30661 tgccaatcgt ccagctgtta agcgtggatt ggaagtaact tatacagaaa ttaaatagca
    30721 aaaaagtcat cttcggatgg ctttttgcta cagttgaggg aaagggtttg cagttatcgg
    30781 cttttgtgct ataattgcta taatatagta aaaagcaaga ggagtgtgag gaagtggaaa
    30841 agaaaattgt gaaggatatc ttatttttat ctcaagtgtc tcagccggca agtcaggagg
    30901 acctttatct tgccagagat ttgcaggata cactcttagc aaatcgtgat acctgtgttg
    30961 gtctagctgc caatatgatt ggggtgcaga agcgcgtgat tatctttaat cttggcttgg
    31021 ttcctgtggt catgtttaac ccagtgcttc tgtcctttga aggatcctat gaggcagaag
    31081 aaggctgttt gtccttggta ggtgtgagat caactaagcg ttatgaaacc ataaggcttg
    31141 cctatcgtga cagcaagtgg caggaacaga ccattacctt gacaggcttc ccagctcaga
    31201 tttgccagca tgagctggat cacttggaag gacgaatcat ttaggaggaa agcaaatgaa
    31261 acgaatagtc tttgaactta tttttatcgc aacgacctgg tatatctttt taccgcccct
    31321 taacctgacc agctgggaat ttctcttctt cctctgtggg catttgttag ttgtggcaat
    31381 cttatttggc tttggcaagg ggataaacct tgtcaaaacg gttcatgtgc gccacggtaa
    31441 ggcggaagct gccttaaatc ttgagggttt caaaatcaat cggttaggga aaattctgtt
    31501 agcttcgatt ggaggaattc ttctcttggc agctttggtt tccttggtaa cttccagcat
    31561 gtttcaggct aaaaattatg ccaatgtagt cacggttacg gaaaaagact ttactgaatt
    31621 tcctaagagt gacaccagta aggttcctat cctagataga agtactgctg aaaaaattgg
    31681 agaccgctac ttgggttccc taaccgataa ggtgtcgcaa tacgtagcgg cagataccta
    31741 tacccaattg acaattgatg ggaaacctta tcgggtcaca ccactagaat atgcagaccc
    31801 tatcaaatgg tttaacaatc aagccaaggg aatcggtgag tatattaagg tggacatggt
    31861 aactggaaat gcggatttgg tggacttgaa gacaccaatc aagtattcag actcggagta
    31921 ttttaaccgt gatgtcaaac gtcacctgcg cttgaagtac ccgaccaaaa tctttaaaac
    31981 tccatctttt gaggtggacg atgagggcaa tcctttctat gtagcaacgg tttaccaaaa
    32041 gcaatttgga cttgctgttc ctcgtcctgc ttcagtcatt atcttggatg ctacaaatgg
    32101 agaaaccaag gaatacagct tatcagatgt tccagaatgg gtggacagga tctatccagc
    32161 agaggaaacc attgagcaaa tcaactacaa cggcaagtac aaggacggtt tcttgaatgc
    32221 catgatttcc aagaaaaacg tgacccagac taccaatggc tataattact tgtctatcgg
    32281 taatgacatc tatctctaca caggtgtgac gtcggctaat gcggatgaga gtaatcttgg
    32341 tttcatcctt gaaaatatgc gaacaggaga aatcactaag tatagcttgg cttctgcgac
    32401 agaagaatca gcccgtgaat cagcagaagg tgctgttcag gagaaatcct acaaagcaac
    32461 cttcccaatc ctcatcaacc tcaatgacaa gcctctctac atcatgggct tgaaggacaa
    32521 tgctggcttg gtcaaagagt acgccctggt agacgcagtc gagtaccaaa atgttatcgt
    32581 tgctactaca gtggaagaga tgctcagcaa gtatgccaat aaaaacgacc ttgaaattga
    32641 caatgcaacg acagaaagca tcaatggagt agtagcagac ctcaaatcag ctgttatcaa
    32701 gggagacact gtctacttct ttaaagttga tggcaagata tacaaggtca aggcttcagt
    32761 atccgatgac cttccttacc ttgaaaatgg taaaaccttc gaaggtcaag taggaaaaga
    32821 caattatctc aagaccttta agctacggta aaaataggtt tttttcagaa agtatatgtt
    32881 ataataaggt aaattaagcc taatggagga caaagaaatg ggtttttatt tgatggttgc
    32941 ttcaatgcta ctcggactgc tcgctttgaa gataggtttt tctcagttca aggagaaaaa
    33001 agataaattc ttatctatct taacaagttt agctggcata gcccttgttc tcgtggctgt
    33061 ctggttagga tggcccaaat aaatagaaaa acctcggtca aaccgaggtt tttcaaatga
    33121 atttcttggt tgtggctaaa aaatatgcta cactatcaat atgaaaattt taatcccaac
    33181 agcaaaagaa atgaacacag acttgccaag tatcgaggca actcctttaa aaccagaaag
    33241 tcaggccgtg cttgatgcct tggctctcta ttctgccagt caattggaga gtttctacaa
    33301 ggtatcagct gagaaagcga cggaagaatt tcaaaatatc caagctttga aaaggcaaac
    33361 tgctcaacac tatccagcct tgaaactttt tgatgggctt atgtaccgca acattaagag
    33421 agataagctg accgaggcgg aacaagatta tcttgaaaat catgtcttca ttacctcggc
    33481 tttgtacggt gttgttccag ccttgtcacc catggctcct caccgtttgg attttttgat
    33541 gaaattaaaa gtcgctggta agactttgaa gagccattgg aaggcagtct atgatgaagc
    33601 tctgaagaag gaagaagtga ttttctctct cttgtcatca gagtttgaga ctgtattttc
    33661 taaggaaatc agagcaaaga tggtgacctt caaattcatg gaggatagag gcggtcaact
    33721 gaagattcac tcaactatct ccaagaaagc gcgcggggcc tttctaacag ctttaataga
    33781 aaatcaagta caaactgtgg gggaagcacg tcgcttgaac tttgctggat ttgtttaccg
    33841 agaagatttg tcacaaccac agggattggt ttttgtaaag gaagtataga aatcagataa
    33901 gagaaaagtc agcacgatta tgctgacttt tttgataact tataaagtat tggacaaatg
    33961 aatctcattt gataaggtgt taagatgatt aaccaaattc atgattgaca gatgatttcg
    34021 gcaaataaac ttcttggtaa tccctgaaat atagaaatta gacacgatga tatcgtaatc
    34081 tgtctgattt aaaatctcag gacttgtttt taattcatcc caaatatcga aggtaaagcg
    34141 gttattacag taataggaaa gcatatcaac aaaggtaagg gatatagcat ggtcaaagtt
    34201 actgataatc aaaaccttga taggtggtcg attttctaac aactgagtag agaggttttc
    34261 agcatgggtt aagatggtgt acatcaagtg ttccaactgt tcaggatggt catgctgtcc
    34321 aattgcctga cggtattgag ccagttcttg acgcgcactt cccataaagt ctgggaagta
    34381 aacttcaaat tttttaatcg tcaatgcttt ttgttcaaat aagattgggg tagaaaagat
    34441 ttcctgtctt tcaaagaaag cagtgttgtg taaatgccaa atcagctcat cgtggttagt
    34501 aaaagtaatc ttatattgtt ttgataggcg ttctaatatt tgactaagta attggtaaga
    34561 gtatcttgct tgactgttat cttctaaaga gttgaagaat tcttgaggat ctaggaaaat
    34621 atcattctga agaaaagaaa taaaaatttg agcgattgta tctggagtca tttcaagccc
    34681 aaaatgttta gagaaataag caagtgtctc ctggaaattt ggaatgctct ttaaaagagg
    34741 ataatattct ttgtaaaagt ggttggggag gtcaatgaag tgaccatttt taaccctgtg
    34801 gatactgata gctatcatca gtttgtacat tctataaatt gaaaatcgca ttggataatt
    34861 cgtgatttta tagaagaagt cagcaaactc tgttaaatct tcctcaggaa ggtcaggaaa
    34921 aggccaatct aggaaataat agcgctcaga aaagtattgg gcaaaaaagt aacgaatatc
    34981 gatctcattt ccacgtatct cggatggagt aaaagataat tcaatttgaa actgagatga
    35041 aagaaccttt gtaatatttc tgcccagtcg atagaggttg gcactactaa agtaaagatt
    35101 ttctattgtt cgataaatag gtagcccctc attgaagaac atatactcta gtaattgaaa
    35161 agattgagaa ttagcaagaa aatattgata aatatcttct acactagtgt taacttctaa
    35221 atctatcatg attccgttaa cagaagattg gatgttaata gatggaaagg caatacgtaa
    35281 ttcatttaaa tcacttttta agattcgttc tgtacatcct aatttttcgg ctagttcatg
    35341 taatttcatc caattacgat tctgaattag ggtttccatt aagcgtaatt gtctttgaac
    35401 ttttgtggat aaaaggtttc tcatacgggt atttcctcca taattcatta tatctttttt
    35461 tgtctaagta gtataaaaaa attagagcaa gaaaattgtc gttagtaatc ggaaaaatta
    35521 gaataatata agtggctttt aagtgcaaaa acagaatata ttaattgttc agaaaatatt
    35581 gattaatgcc tcaaatagtt gtacattgta aactataaaa tactatctag gagtttaaaa
    35641 tgaaacattc acataaaaaa tcatttgact ggtatagcat gcaacaacgt tattctattc
    35701 gtaagtatta ctttggtgca gttagtgtct tgctcggtac cgctttggta ttaggtgcag
    35761 cagctagtgt ccaaacagta caagcggaag aaaacaaaca agaaactacc aatagtattt
    35821 ctgttggtag gggagaagca gctactaaac cagcagaggt ttctgcgtct aataaagaga
    35881 aaacctatgc agctccaact gtagctaatc cagtagaaac gactccagtt aaaactggag
    35941 aagttactaa accagcagaa aaagttgaag aagcaaaaga caaaaaagag gaagtaacgc
    36001 atcaagatgc tattgacaag tcaaaattgt taacggctct ttcgcgtgct aaaaagttag
    36061 aaagcaagtt atatacagaa gcaagtgctg ctaacttgca aacaagtatc caagctggtc
    36121 aaagcttgct tgggaaagca gatgcatctg aagctgaatt atcagcagca gagtcatcta
    36181 ttattggtct agaacttcgt tctaactcta acaaaggaac tgtatcagaa acgcctgtag
    36241 cgaagaaagc tgacacaaca gaagccaaag aagaagctaa gccagctgca agaacgactg
    36301 accgttcagc tgttgatagt agcgttttag caactaatgc acctgccaaa gtagaaacaa
    36361 cttcagctcc aaaatctact aatgaaatct tgaaaccagg tttgaacctt tctgatgctc
    36421 gccaaaatcc agctatccgt aaggaagatg ttgatagagg gtatagtggt tttagaacag
    36481 caggaagtgg ctttagagcg gctggaagtg gttttagagc agctggacca gaaaataaac
    36541 caatcttaaa tccaaataat actattgcat tttctgacat cagtcagggc tcacattcat
    36601 ttcgtggtat aggacattca cgtggtggtc gtgaaatcca ttatgatgta acgacagtac
    36661 gtagaggtga taatattgaa tttacaatta attattctgc gccaggaaat cttagagaat
    36721 ttgttaataa taattttatc ttggataaag gggatggatt tggaaatcct tcaaatgcaa
    36781 cgatcacaag ttcaaatcca agagtaaggg agcaatcaaa atctattagt cagggtgcca
    36841 actacgtatc tcactcagga tacagtatga cctctgctat ttcaacaaat acggaacaaa
    36901 ctatcagatt tagtttgcct atcattaatc taaatggtga tttgtctgtt cgtttgaaac
    36961 ctgttacttt taacgtggac caaggtggtg gtggtgccgc tactagcaat gacccataca
    37021 gtaactctaa ctattattat agagcaaacc cactatactt ggatgcaaac ccttatggtg
    37081 gtactaataa taagactgtt tcagaagaca ttgacttcca aactgtctat cttccaacta
    37141 gtaagttacc agaaggtcaa actagattag ttcgagaagg tgaaaaagga caacgtcaaa
    37201 ttacctataa agttcatcga tttggtaacg aaacactttt aggattgccg attagtaata
    37261 gtgttactaa agaagctaag ccacgtatta tgcaaattgg tgtggctaaa gatctaatcg
    37321 atacagtaaa accacgtgtt gatcaaaata aagtcggtga tacaaataac ctcactttct
    37381 atcttgataa cgatggaaac ggtgtttata ctgaaggtgt agacgaactt gttcaaaaaa
    37441 ttgctattaa agatggagct aaaggtgaaa aaggagacca aggtgaacgc ggtctaactg
    37501 gagctaaagg tgaaaaagga gaccgaggcg aacgcggtct aactggagct caaggagcta
    37561 aaggtgaaaa aggagaccga ggtgaacgcg gtctaactgg agctcaagga gctaaaggtg
    37621 aaaaaggaga ccgaggtgaa cgcggtctaa ccggagctca aggagctaaa ggtgaaaaag
    37681 gagaccgagg cgaacgcggt ctaactggag ctaaaggtga aaaaggagac cgaggcgaac
    37741 gcggtctaac tggagctcaa ggagctaaag gtgaaaaagg agcccaaggt gaacgcggtt
    37801 taactggagc taaaggtgaa aaaggggacc aaggtgaacg cggtctcact ggagcccaag
    37861 gtgaaaaagg agaccgaggc gaacgtggtt taactggagc taaaggtgaa aaaggagccc
    37921 aaggtcaggc aggtcgtgac ggtgtaactc caaccgtaac cgttaaagat aataaaaatg
    37981 acggcactca tactatcact attaacgacg gtagaggtaa tgttacaagt actgttgtaa
    38041 gagatggttt cgatggcgca agtccattag ttgcgactca acgaaatgac gcagataaga
    38101 caacaactgt tatcttctat tacgataaaa atggaaacaa tgaattagat gcttctgata
    38161 agaaattaaa agaagttgta attgcagatg gtgctaaagg tgaaaaaggg gacaaaggtg
    38221 aacaaggtct tcaagggcgt gatggtgaac aaggaccaaa aggagaagat gggaaaaccc
    38281 caacagttaa agtaactgat ggtcaagatg gaacgcatac aattacaatt aacgatggta
    38341 aaggtggtat aactactaca gtagtaagag atggatttga tggtgcaagc cctcttgttt
    38401 ctactcatag aaatgaagca gataaaacaa caactgttat tttctattat gatctaaatg
    38461 ataataatca atttgatgaa ggagatacaa aacttaaaga agttgttatc gcagatggaa
    38521 aacaaggacc aaaaggtgac aaaggtgata acggaaaaga tggattcaca ccagaagtaa
    38581 cagttacaga taacaataac ggaacacaca ccatcacaat cacacaacca gacaacagac
    38641 catcattaac aacaatcgtt aaaaacggtg aagatggaaa aacaccaaaa gtcaaagcag
    38701 aacgagatga tgcgaagaaa caaacaacat taacattcta cattgacaaa gatggagatg
    38761 gaagttacac agcaggaaaa gacgagttag ttcaaacaac agtagttaaa gacggacaag
    38821 acggagctgc aggagcatct ggacgtgatg gaaaagaagt attaaacgga aaagtagacc
    38881 caacaacaga aggtaaagat ggagacacat tcgtaaatac acaaacagga gatgtattcg
    38941 ttaagaaagg taacacttgg gaaccagcag gaaacatcaa aggaccgaaa ggtgacaaag
    39001 gtgcagatgg tgccaagggt gaaaaaggag cccaaggaga acgcggcctg actggagcgc
    39061 aaggtgtcaa gggtgaaaag ggagatcagg gtgaacgcgg tctaactgga tctaaaggtg
    39121 aaaaaggaga ccaaggtgaa cgcggtttaa ctggagcgca aggtgccaaa ggtgacaaag
    39181 gtgaacaagg tcttcaaggt cgtgatggag ctcaaggacc aaaaggcgca gatggacaaa
    39241 gaggaccagc gggaccacaa ggaccaaaag gagaacaagg taatccagga actccaggta
    39301 aagatggaaa atctctaatt gctgttaaaa atggaactga aacaaaagtc tatgtagagg
    39361 atccggctag accaggacaa ccacttaatc caaaccaacc attagcaact atcactgatg
    39421 gaagaaacgg tactaatgga caatcaccaa caattacagc gacacgaagt gtgcaaaatg
    39481 gcaaaaatgg tgtattagta acaattacac cagtagaagg tcgtccacaa acaacatttg
    39541 tagaggatgg acaaaagggt gctgatggga aaactccaac agtaacaata actgaggggc
    39601 aaaacggcac acatacatta acggttcata atccaggaag tccagatgtg acaactacga
    39661 tccgtgatgg agctacagga caagcaggtc gtgatggtaa agatgtatta aacggaaaag
    39721 taaatccaca accaaaccaa ggtaaaaatg gagataaata tattaatacc gaaaccggtg
    39781 atgtctatgt taaaaacaat ggaaactggg ataaagaagg caacatcaaa ggccctaaag
    39841 gtgacaaagg tgcacaaggt gctaaaggtg aaaaaggagc ccaaggtgaa cgcggcctaa
    39901 ctggagctca aggagctaaa ggtgcggatg gcgcagtagg tcgtgatgga cgtgacggta
    39961 aagacgtgtt gaacggcaaa gctaacccag aagcacatca aggtaaagac ggcgataaat
    40021 acgttaatac agaaacaggc gacgtcttcg ttaagaataa cggcaactgg gataaagagg
    40081 gcaacatcaa aggccctaag ggtgacaaag gtgaacgcgg agaagatggt aagactccag
    40141 aagtaactgt aactccaggt aaagatggcc atagtactga cattacattc actgttccag
    40201 gtaaagatcc agttacagta aatgttaagg acggagaaaa tggtctgaac ggtaaaactc
    40261 caaaagttga tttacttcgt gtccaaggaa aaaacggaaa tccatctcat acaattgtga
    40321 cattctatac agatgaaaac aatgacggca aatatacacc aggaactgat gaacttctag
    40381 gttcagaaat gattaaagat ggtgctaaag gcgcggacgg acgagatggt aaatcattgc
    40441 ttactgtcaa ggatggtaaa gaaactaaag tttaccaaga agatccagct aacccaggac
    40501 aaccattaaa tccagaaaaa ccacttgcgg taattagaga tggagtagat ggaaaatcac
    40561 ctacagttac agctgttcgt aaagatgaag cagggcataa aggtgtagaa atcactgttg
    40621 ataaccatga tggttcacaa ccaactacag tctttgttca agatggtgct aagggagaaa
    40681 ctggtgcaac cggtcaggat ggacaaactc ctacaatcac tactcaacgt ggacaagatg
    40741 gccaaagcac tgttgtaact atcacaacat caggtaaaga tccagtaacc ttcactgtaa
    40801 aagatggtaa gaatggtaaa gatggccgtg caccgaaaat caaagtagaa gatattactt
    40861 caccttcaag aattagacgc gatacagatg ctgctgcaac tccaacgcgt aacggtatcc
    40921 gtgttacagt ttatgatgat gttaatgaca atggggtata cgacgaaggt gtcgataaag
    40981 tattaaatag taaagatatt tataacggta tagatggacg tgatggttca gctccaacta
    41041 ttactacaaa agataatgga gatggaactc acactatcac agttcaaaac ccagatggtt
    41101 ctgaatcaac aacagttgtt aaagatggta aagacggtaa aactgcgaat atcactacaa
    41161 cagaaaaccc agatggaagc cacacaatta cggtgacaaa tccagatggt tcaactaaag
    41221 aaactgttgt taaaaacggt aaagacggta agactcctaa agttgaagta acggataaca
    41281 acgatggaac tcatactgtt aaagttacag atggagacgg caatgttacc aacgctatca
    41341 tcaaagatgg taaagacggt aaagctgcaa cagcaacaac tactgaaaat ccagatggaa
    41401 gccacacagt aacaatcact aacccagacg gaactaagaa tgagtttgtt gttaagaatg
    41461 gacgtgacgg tgttgacgga cgtactccaa ccgcatctgt tcgtgataat ggagacggaa
    41521 gtcatacaat cgttattaca aatccagaag gtgtgacaac tgaaaccaca gttcgtgatg
    41581 gtaaatcacc aaaagtgact ataactgatg aacaaaatgg aactcataag atctctgttc
    41641 taaatggtga cggaacaact acaacaacta cagttcgtga tggtagagaa ccaaaacttg
    41701 aagttattga taacaacgat ggttcacaca ctattaaagt gacaggtgct gatggtaaag
    41761 gaacgacaac tacaatcttt gatggtaaat caccaaaagc gaacatcgtt gataacggag
    41821 atggaactca tacattaaca atcgtagatt ctgatggtcg tgaatacaaa tctattatca
    41881 aagatggtaa agacggtaaa gatagcgttt caccaactgt aactgttaaa aataataact
    41941 atggaactca cgttgttaca atcactaatc cagatggaag taagacagaa atggtgatta
    42001 aagacggtaa agatggtaaa tcaccaaaag tttctgttga agataatggt gatggtagtc
    42061 atacaatcac aatcatcaat tctgatggaa ctgtgacaaa aacagttatt aaagatggta
    42121 gagatggacg tgatggtcga gacggcaaag acggtaaaga cggtaaagat ggaaaatgtg
    42181 gatgccaaga caaaccagta acaccatcaa atgacaaacc agttcctcca acaccaaatg
    42241 tgccgacacc agaagtaccg gttaaacctg tgccagcgca accaacacca aatgtaccga
    42301 caccagaagt gccagtacaa ccaactccag ctgttccaac accagaagta ccggttaaac
    42361 cagtaccagc ggttccagaa caaccagtag taccaacacc ggctcaacca gcaactccag
    42421 taaatgctaa cccagtagca ccaactacag gtaaagaaaa ccgtggggac aaattacctg
    42481 aaactggaag ccaatctgat tatatctctg ttcttttagg tagcggtatt ctattgagcc
    42541 tatatgtagg acgaagaaaa gaagcttaat cagccaaaag cagtcactaa tttctcgaaa
    42601 gaagatgata agtgatagat ctcgaatgaa agaatattct tagaatgatt ttcctatgat
    42661 ggaaaagact gctaaaaacc ttgagtggta acgctcaagg tttttttgga tggctgatgt
    42721 ttgtctgaaa atcttgattg agaaataaaa agccacccta ttaggtggct ataattgatt
    42781 tagactttct aaaattttct attttttact gaatcgcttc gaccaagtct tgagcttgtt
    42841 tttcaagtga gtttaggact gtttcttcaa gaaccaattt tccgtctgcc caggcagagt
    42901 cattaacacg tgcagcagtg aaatcaccaa cgccttgtgt acggataaat ggcaagaggt
    42961 ctttgtagat agcgaaaagt tgatcgtgcc ctgcattggc tacagatgat actgtgacaa
    43021 acttgtcttg aagggcagaa acgccacgtg tatcagacaa gtcaagggca cgagatagcc
    43081 agtcaagcaa gtttttcact gtaccaggga tagagaagtt gtagactgga gagaaaatcc
    43141 agatagcatc cgcaacgaga actgcttcac gagcagcagc tacagctgga tgtgttggaa
    43201 cttccaaatc ttggctgaag agaggaaggg ctgaataatc aaggtagcta acttccgctt
    43261 taccagcaag tgctttctca gcttcgagcg ccatttggtg gttgaaagaa ccttgacgta
    43321 gtgatccgac gataaataat acttttttag acatgatgtg tctccttttt cttaaaattt
    43381 cgagaactct ctcgttgtta taaaatatat tacaacaaaa caaaagaact tgcaagaaat
    43441 ttgctcagag tttatttgaa aattgtaaaa tttcggaaac tctagtcatt tgtgactgaa
    43501 aaagtaagaa ctgtttcact ttcaaaatcg gttttgtttg aagggagaaa tagaattggt
    43561 tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag agattttcga
    43621 agagtattct tatattcact tgtaataaag cttaatagat ggtaaaatag acgagtgaaa
    43681 aaaagacaaa acaaagcaaa aaataatcta ctgtggcagt atggtctagg gatgacgatt
    43741 ttgtttgtgg ttatcagtgc ttcctttctg tatatggttt ctcttagcat gaaaccctat
    43801 caaacagcta aaagtgaagg agaaaaatta gctcagcagt atgcaggatt agagcaggtc
    43861 gatcaggttg atttatacaa tggcttggaa tcttattaca gcgttcttgg tcgtaataaa
    43921 cagcaagaag cgcttgctgt cctgattggt aaagatgatc ataagattta cgtttatcag
    43981 ctaaatcagg gtgtttcaca agaaaaagca gaaacggttt ctaaggaaaa gggagctggc
    44041 gagattgaca agataacctt tggccgttat caagataagc caatctggga agttaagtca
    44101 ggatctgatt tttatctagt agattttgaa acaggagcat tggtcaacaa ggagggccta
    44161 tgaaactatc caaccgtgtt ttagaaatgg aagaaagtgt gactttggct gctggagccc
    44221 gtgccaaagc actgaaggct gagggtagag atattttgtc tctaaccttg ggtgagccag
    44281 atttcactac tcccaaaaat atccaagatg ccgccattgc atcgattcga gatgggcgtg
    44341 cttcttttta tacagtaacc tcaggtctgc cagagcttaa ggcggtggtc aatagctact
    44401 ttgagcgctt ttacggctat tctgtagcgt caaatcaagt gacagtcgct gcgggagcca
    44461 aatattctct ctatagcttc tttatggctg tggtcaatcc aggtgatgaa gtcatcatcc
    44521 caaccccata ctgggttagc tatggagatc aggtcaagat ggcagaaggc gttcccgtct
    44581 ttgtttctgc taaggaagac aatcacttta aggtgaccgt agagcagtta gaagcagctc
    44641 gcactgacaa gaccaaggtt ttggtgctga attcgccatc taatcccaca ggtatgattt
    44701 acacccgtga ggaactcttg gcaattggaa actgggctgt agaaaatgat attctcatcc
    44761 tagcagatga tatctatggc cgcttggttt ataatggtca tgagttcaca ccgatttcta
    44821 gcctatcgga agcgattcgc aagcaaacag tggtcatcaa tggtgtgtct aaaacctatg
    44881 ccatgactgg ttggcggatt ggttatgccg ttggagaagc agacattatc gctgccatgt
    44941 ccaagattgc aggtcaaaca acttcgaatc cgtcagcagt agcccaatat gcagcagttg
    45001 aggctctatc aggtgagcaa gatactgtag aaagcatgcg tcaggccttt gaggaacgtc
    45061 ttaataccat ctatcccctc cttgcagagg tgccaggatt tgaagtggtc aaaccacaag
    45121 gggccttcta tctcttccca aatgtcaaaa aggccatgga gatgaaaggc tacacggatg
    45181 tgacagactt tacaactgtt atcttagaag aagccgaagt ggccttggta acaggagctg
    45241 gatttggagc accagaaaat gtccgcctca gctatgcgac agacctagac acgcttaaag
    45301 aagcagtcga acgcttgaaa gcatttatgg gtagtgagaa tgattgatca ttttgagatt
    45361 aaggtaaagg atttacaaat tttagaagga ttttatagga gttttctcgt tcctttggac
    45421 tataaattgg cttttaagac tagttctcta attagttttc tatccccgaa cagccctcat
    45481 cctggtggtg atttttggct gactcaggga acacaagatc ctgttcattt tgctttttta
    45541 gcagaaaata aggaagaagt tcaggcttgt tatgaggctg gcatagaggc aggtgggcga
    45601 gacaatgggg ttcctggtta tcgaagtgag catccgattt actatgctgc ttttatgatt
    45661 gacctggatg ggaacaatat agaggtggtt tgccataaag aataaaaaag aaaaggaaaa
    45721 ataatgacaa aacgtgtaac gattattgac gtaaaagact atgttggtca ggaagtgacg
    45781 attggcgctt gggttgccaa caaatcagga aaaggaaaaa tcgctttctt acaattgcgt
    45841 gatggaacag ccttctttca aggtgtggct tttaaaccaa actttgtcga aaaatttggt
    45901 gaagaagtgg gacttgagaa gtttgatgtt atcaaacgct tgagccaaga aacgtctgtt
    45961 tatgtgacag gtattgtcaa agaggacgaa cgttctaaat ttggctatga gttggacatc
    46021 acagacatcg aagtgatcgg tgaatctcaa gactacccaa tcacaccaaa agaacacgga
    46081 acagactttt tgatggataa ccgtcacttg tggctacgct ctcgtaagca agtagctgtg
    46141 ttgcaaatcc gtaacgctat tatctatgca acttatgagt tctttgacaa gaacggtttt
    46201 atgaagtttg acagcccaat tctttcagga aatgcggcag aagattctac agaactcttt
    46261 gaaactgact acttcggaac gccagcctac ttgagccaat caggtcagct ttacctagaa
    46321 gcaggggcta tggctcttgg tcgtgtcttt gactttggtc cagttttccg tgctgaaaaa
    46381 tcaaaaacac gccgtcactt gactgagttc tggatgatgg atgctgagta ctcatacttg
    46441 acacatgatg agtcgcttga cttgcaagaa gcttatgtga aagctcttct acaaggtgtt
    46501 cttgaccgcg cgcctcaagc cttggaaacc ttggaacgtg atacagaact cttgaaacgc
    46561 tacattgcag agccattcaa acgtatcact tacgatcaag ccattgacct cttgcaagag
    46621 catgaaaatg atgaagatgc tgactacgag catcttgagc atggtgatga ctttgggtca
    46681 ccacacgaaa cttggatttc aaaccacttt ggtgtgccaa catttgtcat gaactatcca
    46741 gcagccatca aggccttcta catgaaacca gttcctggaa atccagagcg cgtgctttgt
    46801 gcagacttgc ttgctccaga aggctatgga gaaattatcg gtgggtctat gcgtgaggaa
    46861 gattacgatg cccttgtcgc taagatggat gaacttggca tggatcgtac agaatatgaa
    46921 ttctaccttg accttcgtaa atacggtaca gttccacacg gaggatttgg tatcggtatc
    46981 gaacgtatgg taaccttcgc agcaggaaca aaacatatcc gtgaagctat tccattccca
    47041 cgtatgttac accgtatcaa accataagaa caggaaaacg cccatgtggc gttttttcga
    47101 attctagtgt ttttacttgc gtaaaaaaat tttttctagt ataatagttt attgtgagcg
    47161 aacctcactt accccttgca aagtcttggg gtcattagac caaaaggagg aacatatcaa
    47221 tggctaaata cgaaattctt tatatcattc gtccaaacat tgaagaagaa gctaaaaacg
    47281 ctttggtagc acgttttgac tctattttga ctgacaacgg tgcaactgtt gttgaatcaa
    47341 aaacttggga aaaacgtcgt cttgcatacg aaatccaaga tttccgtgaa ggactttacc
    47401 acatcgttaa cgttgaagca aatgacgatg cagctcttaa agagtttgac cgtctttcaa
    47461 aaatcaacgc tgacattctt cgtcacatga tcgtcaaaat tgacgcgtaa gaaggtaaac
    47521 tatgattaac aatgttgtac ttgtagggcg tatgacacgt gacgctgagt tgcgttatac
    47581 cccatcaaat gtagcagttg cgacttttac tcttgcagta aaccgtacat ttaagagtca
    47641 aaatggtgaa cgtgaggctg attttatcaa tgtcgttatg tggcgccaac aggctgaaaa
    47701 tcttgctaac tgggctaaaa aaggctcact tatcggggtg acaggtcgta tccagactcg
    47761 tagttacgat aaccagcaag gacaacgtgt ctacgtgaca gaggtcgtgg ctgagaattt
    47821 ccaaatgttg gaaagccgta gtgtgcgtga gggtcacaca ggtggagctt actctgcacc
    47881 aactgcaaac tattcagcac ctacaaattc agtaccagac ttttcacgta atgaaaatcc
    47941 atttggagca acaaacccat tggatatttc agatgatgat ttaccattct aatggacaac
    48001 taaacttata aaggagaaaa aacatggctc aacaacgtcg tggcggattc aaacgccgta
    48061 aaaaagttga ttacatcgca gcaaacaaaa ttgaatatgt tgattacaaa gatactgagc
    48121 ttcttagccg tttcgtttca gaacgtggga aaatccttcc tcgtcgtgta acaggaactt
    48181 cagctaaaaa ccaacgtaaa gtaacaacag ctatcaaacg cgctcgcgta atggctttga
    48241 tgcctttcgt aaacgaagat taaagaaaag acccggttgg gtctcttttt catgagcttg
    48301 aaaacaagaa agcgaattaa gaccccaacg ggtctttttt cacaagcttt gaaatgagag
    48361 agcgagtcag gctcagtggc tcccttttta cgagtttgaa aacaagagaa cacattaaaa
    48421 gcacgatgga gctttttcat aaatatgtct tttttctcat ccaagaaatt aaaataaaaa
    48481 tgataaactt tttgaaaata ggggatttgc ctactttatg atataatgga aagagttaga
    48541 ttgaaagagg tagtgagatg gacgcgaaat taagatacaa ggcaaagaag atcaagattg
    48601 tcttttttga tattgatgat acattgcgga attcaaagac agggtttatt ccaactacaa
    48661 ttcccactgt ttttaaacag ttgcgtgaaa aaggaatttt aacaggaatc gcctctggac
    48721 gtggcatttt tggtgttgtt ccagagattc gtgatctcaa gcctgacttt tttgtaactt
    48781 tgaatggggc ttatatcgaa gataaaaaag gtcaggtcat ttatcagcat cagattgaaa
    48841 agtcagatgt tgaggagtat atctcttggg ctaagcaaga aggaattgag tatggcttgg
    48901 ttgggagtca tgatgccaag ttgtcgactc gcaccgatat gatgagtgaa gctatcaatc
    48961 caatttatcc cgacttagat gtagatcccg atttccatga aaaagaagat atctatcaga
    49021 tgtggacttt tgaagataag ggagatgact tgcacttgcc tgacagtctc tcagacaaac
    49081 ttcgcatggt tcgttggcat caacattcgt ctgatattgt gccgatttca ggctccaaag
    49141 cgacgggggt ggaaaaggtt gtggaacacc ttggcttgaa accagagaag gtcatggttt
    49201 ttggagatgg tctcaatgac ttggaactct ttgattatgc tggaatcagc gttgccatgg
    49261 gaatttctca tgataaaatc aaagaaaaag cagattatat tacaaaaaca ttagaagaag
    49321 atggcatttt tgctgcctta gaagtatttg gtatggtaga aaaagaattg cattttccac
    49381 aagtagacat tgaaacagta gaaggtcctc ttgcgactat taagaccaat cacggagact
    49441 tacgtatcaa gcttttccct gaacatgctc ctaaaacagt ggctaacttt gtatctcttt
    49501 caaaagatgg ctactatgat ggtgtcattt tccaccgtat tatcaaggac tttatgatcc
    49561 aaggtggaga cccaactgga actggtatgg gtggcgagtc aatctacggc gaatcttttg
    49621 aggatgaatt ctcagaagag ctttacaata tccgtggtgc tctttccatg gcaaatgctg
    49681 gtccaaatac caacggcagc cagttcttta tcgtgcaaaa ccaacaccta ccttattcga
    49741 agaaagaaat tactcgtggt ggttggccag aaccgattgc agaaatctat gccaatcaag
    49801 gtgggacacc tcacctagac cgccgtcaca cggtttttgg tcagttagct gatgaagcat
    49861 cttacgctgt cttggatgcc attgctgctg ttgagacagg agctatggac aagccagttg
    49921 aagatgttgt aattgaaact attgaaatcg aggactaaga tgaaaatcgg tgataagcta
    49981 aaggggcgta ttacagggat tcagccctac ggtgcctttg ttgagttaga gacgggtgat
    50041 acggggctga ttcacatttc agaaattcgg acaggattta ttgaaaatat tcatgaaacc
    50101 ttgaaagtcg atgaagaggt tcaagttcag gtagtggatt tagatgaatt tacggggaaa
    50161 gccagtcttt ctatccgcac tttggaggaa gaaaagtacc agtttcctag acggagacgc
    50221 ttttcaagcg accgctttaa ctacggtttt gctccctttc gacgaatgtt accaatctgg
    50281 actggagaag ccctgcacca tttaaaaaag aagaagtagt ttagagtttt atattttttg
    50341 taatgttaat ataaatttgc tataatagga ccatggaaga agaacaatta ttaaaatcag
    50401 gagagcgcat taaccagctc ttttcgacag atatcaaaat cattcaaaat agagaggttt
    50461 ttagctattc ggtggatagt gttctcttat cacgatttcc acgttttcct aagaaggggc
    50521 tgattgtgga tttctgtgct gggaatggag cagtggggct ttttgctagc actcgtactc
    50581 aagcacagat attgtctgtt gagattcagg agcgtttggc ggatatggct gaacgctctg
    50641 tccgtttgaa tggattagag gagcaaatgc aggtcatctg cgatgatttg aaaaatatgc
    50701 ctgctcacat tcaggggagt aaggtggata tgattttgtg taatccaccc tatttcaagg
    50761 tggatcctta ttctaatctg aacgagagtg aacattacct cttggctcga cacgaaatca
    50821 cgactaattt ggaagaaatc tgtcgtagtg cccagagtat tctcaagtct aatgggcgtt
    50881 tggccatggt tcatcgtcct gatcgacttc tggatatttt ggatacgtta aaacgacata
    50941 atctagcccc taagcgcctg cagtttgttt atccaaaaag agaaaaggaa gccaatatgc
    51001 ttttgattga ggcgatcaag gatggctcaa caagtggctt taaggtctta cctcctttga
    51061 ttgtccacaa tgatgatggc tcttatacgc ccgaaatcga agagatttat tatggatcat
    51121 aaggcttata tgtacgttct ggagtgtcgt gatggatcct actatatagg ctatacgact
    51181 gatgtgagaa gacgcctcgc tatccacaat agtgggaagg gagccaaata tacacgagca
    51241 cgcttgccag tcaaacttat ctatgctcaa ggttttgcca gtaaggaaga agccatgtcg
    51301 gctgaagctc ttctcaagcg taagaagagg ccacagaagg aagaattttt atctgaaaat
    51361 caagatagaa atttactcag ttattttgaa gaatcgtggg gagtcctttg actcctctta
    51421 cctttgtcaa aaagcgaaaa aaatgctaca ataaagaaaa taaacaaaat gaggtattat
    51481 catgtccaag attctagtat ttggtcacca aaatccagac tcagatgcca tcggatcatc
    51541 tgtagctttt gcctaccttg caaaagaagc ttacggtttg gatacggaag ctgttgccct
    51601 tggaactcca aatgaagaaa cagcctttgt cttgaactat tttggtgtag aagcaccacg
    51661 tgttatcact tctgccaaag cagagggagc agagcaagtt atcttgactg accacaatga
    51721 attccaacaa tctgtatcag atatcgctga agtagaagtt tacggtgttg tagaccacca
    51781 ccgtgtggct aactttgaaa ctgcaagccc actttacatg cgtttggagc cagttggatc
    51841 agcgtcttca atcgtttacc gtatgttcaa agaacatggt gtagctgtgc ctaaagagat
    51901 tgcaggtttg atgctttcag gtttgatttc agataccctt cttttgaaat caccaacaac
    51961 acacccaaca gataaaatca ttgctcctga attggctgaa ttggctggtg tgaacttgga
    52021 agaatatggt ttggcaatgt tgaaagctgg taccaacttg gctagcaaat ctgctgaaga
    52081 attgattgac atcgatgcta agacttttga actcaacgga aataatgtcc gtgttgccca
    52141 agtgaacaca gttgacatcg ctgaagtttt ggaacgccaa gcagaaattg aagctgcaat
    52201 gcaagctgcc aacgaatcaa acggctactc tgactttgtc ttgatgatta cagatatcgt
    52261 caactcaaac tcagaaatct tggctcttgg tgccaatatg gacaaggtcg aagcggcttt
    52321 caacttcaaa cttgaaaaca atcatgcctt ccttgctggt gccgtttcac gtaagaaaca
    52381 agtggtacct caattaactg aaagctttaa tgcgtaagat tttgggtgtc agctcaaaat
    52441 cggaaagtct agtttgcctt atatcgaaag gagtttcggc tccttttttt ctaggagtga
    52501 agtatgttag aaaatggcga tttgattttt gtgagagatg ggtcagacat gggacaggcc
    52561 atccagactt ccacaggtaa ctatagccat gttgccattt atttggatgg gatgatttat
    52621 catgctagtg gacaggctgg tgttgtctgt caagaaccgg cagacttctt tgagtccaat
    52681 catttgtacg acctctatgt ttatccagaa atggatatcc agtcggtgaa ggaaagagct
    52741 tgcaaacatc ttggagcacc ctacaatgct tctttctatc cagatgcacc tggtttctac
    52801 tgctcccagt atatagcaga aatcctacct atttttgaaa ctattcctat gaaatttgga
    52861 gatggggagc aggagattag tgatttttgg agggagtatt acatagaact aggtctgcct
    52921 gttcctctga accaagctgg taccaatcct agtcagttgg cagcatcgcc tctgttacaa
    52981 tgtaaagaaa ggaatcttca tgattcagat ttttaatcca tctcgtttga cgagacagcc
    53041 atttttggag aattgatccg ctatctggac cagtatgagg atgtgattct acgggaaatt
    53101 aaggctcaat ttccagatgt tgcagttgat aaactcatgg aagagtatat aaaggcaggc
    53161 ttgattctac gtgaaaataa gcgctattac ctcaattttc ctacgcttga atcacttgat
    53221 agtcttgaac tggatcaaga gatttttgtc agagaagcta gtccggtcta tcaagccttg
    53281 ttggagcaga gttttgagac ggaattgcgc aatcaaatca atgcagctat tttagttgaa
    53341 aagacggact ttgcgcgcat taaaatgacc ctgtccaatt atttttacaa ggtcaaacag
    53401 cagtatcctt tgacagaaaa acagcaggag ctctatgaca ttttaggaga tgttaatcct
    53461 gagtatgcac tcaagtatat gacggctttt ttgttgaaat ttctcaaaaa agaccagctt
    53521 atgcagaaat gccgtgatat ctttgtggac agtttggttg tcttaggcta tattgtgcaa
    53581 aatgaagatg gaaagtatga gttggctatc gattttgata aggagagatt aactttctac
    53641 ttagcgtgat ttcttgtttc tgagtacatt gtttgacttt ccttagtatt cggtataaac
    53701 tatatgtaac cggtaacaca tatcggaata aactaaagga gacaatcata tgtcacttga
    53761 aaacaaattg gaacaagcaa caggcgctgt caaagaaggt tttggtaaag ttactggaga
    53821 cagcaagaca gaacttgaag gagctgttga aaaaacagtt gctaaggcaa aagacgttgt
    53881 agaagacgca aaaggtgctg tagaaggtgc cgttgaaggt ttgaaaaacg tttttactaa
    53941 agaataggaa aaaatcaagg gtttcatttt cccttgattt tttctattct tataaataat
    54001 tttctgcgac ggctgtatct cctgggtagg attctttctt gccctggatg atttggtaac
    54061 aatcggctcc cttacccgca ataataactg catctaattc gtgatttgtg atagccattg
    54121 ccgccttgat ggcttcttgg cgatccgcaa tcttctcaac aggatgattg atgtagctac
    54181 taatttcatc tgcaatggcc attgggtctt catagttagg gtcatcagca gtcagaaaga
    54241 cttgaatctc agggtgttga ttgaggagga ggccaaagtc cttacgacga ctttctccct
    54301 tgtttcctgt tgatcccaga accagagcaa tctttccggt ttgatgagtt tcaaccacat
    54361 tgatgagttt tttcagacta tccccattgt gggcatagtc gatgaagacc ttggctccat
    54421 ttttctgagt gaggacttcc atacgaccag gaacgcgggt tgcagcgatg ccttttttga
    54481 tgtcctcaag acttgctccg agacggagac aagcaagtcc agcagcaact gcattttctt
    54541 ggttgaagtt gccaatgagt tggatatcat aatctccagc gagtttaccc gtagctgaaa
    54601 agctaaaggc tttggaattc tcgatttggt tatcaaattg gctaccatag aaatcatggt
    54661 cttgatcttc aacctgttct ttcaagactg agaagtggtc catgtcactg ttaatgatga
    54721 ctgctcggct attttccatc aagagacgct tgtggtagaa atagtcttca aagctagggt
    54781 gttcaatcgg gccgatatgg tctgggctga tattgaggaa aactcccaca tcaaaggtta
    54841 gaccatagac acgtctgacc agataggatt gactggagac ttccatgatg aggtgggtac
    54901 ggtcattttg cacagcctga ttcatcatgt caaagaggtc aatactctca ggggttgtca
    54961 acgctgactt aaagaaagtc tcgccatcaa gagttgtgtt catggtcgac aacatagcag
    55021 gtcgatgccc ttgagataag atgttatagg cgaaataggc tgctgttgtc ttacccttag
    55081 taccagtaaa ggcaaggagt ttgagttttt cctgtggatt accatagaac tccatggcaa
    55141 tcaaactcat ggctttcttt atatcgttca caatgatgac agggataccg acttcgtagt
    55201 ccttttcagc tacataccaa gctaagcctt gtgttatagc agaaagaagg tattcttttt
    55261 taaaggcagc gccttttgca aaaaaaagag tgtcttctgt tacttttcgg ctatcgtagc
    55321 tgatgctatc aaaaacaact ttgctgtagt tgtagtggta atgaccttgg tcaataattt
    55381 cgcgaaaaag gccatctttc tttaaaatat ctaatacagt ttcaattttt atcatacttt
    55441 ctattgtaaa ccgaaagtcg caaatttaca agtaacaagg aaaagtttat aatggaagat
    55501 aaggagtttt tcctagttat caaaattgaa tgaggaatct atgtcgcacg aaaacaatca
    55561 ccagcaggcc cagatgttac gggggactgc ttggctaacg gctagtaact ttatcagtcg
    55621 cctactcggg gctgtttaca ttatcccttg gtacatctgg atgggggctt atgcagctaa
    55681 ggcaaatggt ctctttacca tgggttacaa tatctatgct tggttcttgt tggtttcaac
    55741 agcggggatt ccagttgcgg tggccaagca agttgccaag tataatacca tgcgagaaga
    55801 agagcatagc tttgccctga ttcggagctt cttaggcttt atgacaggac taggcctggt
    55861 ttttgcttta gtcttgtatg tctttgctcc ttggctagca gacttgtctg gcgtgggcaa
    55921 agacttgatc ccaatcatgc aaagcttggc ttggggagtc ttgattttcc cgtctatgag
    55981 tgttatccga ggatttttcc aagggatgaa taacctcaaa ccttatgcca tgagccaaat
    56041 tgctgagcag gtcattcgtg ttatctggat gctcctagca acctttatca ttatgaagct
    56101 cggttcagga gattatctag cagccgttac ccaatcaacc tttgctgcct ttgtcggtat
    56161 ggtagccagt tttgcagtct tgatttattt ccttgcccaa gaaggttcac tcaaaagagt
    56221 ctttgaaaca ggagataaga ttaacagtaa gcgtctcttg gttgatacca ttaaggaagc
    56281 cattcctttt atcctgacag ggtctgccat ccagctcttc cagattttgg atcagctgac
    56341 ctttatcaat agtatgagct ggtttaccaa ctacagcaat gaggacttgg ttgtcatgtt
    56401 ttcttatttc tcagccaatc ctaataaaat cacgatgatt ttgatttctg taggggtttc
    56461 gattgggagt gttggtttgc cacttttgac ggaaaactat gtcaaggggg acttgaaagc
    56521 agcttctcgt ctggttcagg acagtctcac cctactcttt atgttcttgc taccagcaac
    56581 ggttggagtg gttatggtag gagaacctct atatacagtc ttctatggta agccagatag
    56641 tttggctctg ggcttatttg tctttgcagt tttgcagtct attattttag gcttgtacat
    56701 ggtcttgtct ccaatgcttc aggccatgtt ccgcaaccgc aaggccgttc tctattttat
    56761 ctatggttct attgccaagc tagtcttgca actacctacc atcgccctct tccacagtta
    56821 tggtcctttg atttcaacaa ccattgctct catcattcct aacgtcttga tgtatcggga
    56881 tatttgtaaa gtaactggtg tcaagcgcaa ggtgattttg aagcgaacca ttttaatcag
    56941 tttgctgacc ctagtcatgt ttctgttaat aggaaccatc cagtggctgt taggattttt
    57001 cttccaacca agtggacgtt tgtggagctt cttttatgta gctcttgtcg gtgccatggg
    57061 gggtggactt tatatggtta tgagtctgcg tacctattta ttagataagg taataggaaa
    57121 agcccaagca gatcgcctgc gagcaaaatt taagctttcg taaggtagca tttataaata
    57181 aaaggaataa gttgaacaat cttttaggag aacttgaaac ttattccttt tttagcgtca
    57241 aaatagctaa aattatcttt caaaaataga aaatgaggtt tttttgattt tttggaaaaa
    57301 acaattgact aaacaaacca agagttgtat aataaaacaa atatttaaag caggaggttc
    57361 tggaaatgaa aaagtctaag agcaaatatc taaccttggc aggtcttgtc ctgggtacag
    57421 gagttttatt gagcgcgtgt ggaaattcta gcactgcatc aaaaacctat aactatgttt
    57481 attcaagtga tccatctagt ttgaactatc tagcagaaaa ccgcgcagca acatccgata
    57541 ttgttgcaaa tttggtagac gggttattag aaaatgacca atatgggaat attattccat
    57601 cattagcaga ggattggact gtttctcagg acggtttgac ctatacctac aaacttcgta
    57661 aggatgccaa gtggtttact tctgagggag aagaatatgc gcctgtaact gcccaggatt
    57721 ttgtgacagg tttgcaatat gcagctgata aaaaatcaga agctttgtat ctagtgcagg
    57781 actctgttgc tggtttggat gactatatca ctggtaaaac aagcgacttt tcaactgtcg
    57841 gtgtcaaggc acttgatgac caaacggttc aatatacttt ggttaaacca gaactttact
    57901 ggaattcaaa aacacttgca acgatacttt ttcctgttaa tgcagatttc ctgaaatcaa
    57961 aaggggatga ttttgggaag gcggatccat ctagtatttt gtacaatgga cctttcttga
    58021 tgaaagcact tgtctcaaaa tctgctattg aatataagaa aaaccctaat tactgggatg
    58081 ctaagaatgt ctttgtagac gatgtgaaat tgacctacta tgatggtagc gaccaagaat
    58141 cactggaacg taattttaca gctggtgctt atactacggc tcgtcttttt cctaacagct
    58201 ccagctatga agggattaaa gaaaaataca aaaacaatat catctatagt atgcaaaatt
    58261 caacttcata tttctttaat tttaacctag ataggaagtc ttacaattat acttctaaaa
    58321 caagtgacat tgaaaagaaa tcgactcagg aagcagttct caataaaaac ttccgtcagg
    58381 ctatcaattt tgcttttgac agaacatctt atggggctca gtctgaaggg aaagaaggtg
    58441 caacaaagat tttgcgtaac ctagtggttc ctccaaactt tgtcagtatc aagggaaaag
    58501 actttggtga agttgtagcc tctaagatgg tcaactatgg taaggaatgg caaggtatca
    58561 actttgcgga tggtcaagac ccttactaca atcctgagaa agccaaggct aagtttgcgg
    58621 aagctaagaa agaactcgaa gcaaagggtg ttcaattccc aatccacttg gataagactg
    58681 tggaagtaac agataaagta ggcatacaag gagttagttc tatcaaacaa tcaattgaat
    58741 ctgttttagg ttctgataat gtagtgattg acattcagca attaacatca gatgagtttg
    58801 acagttcagg ctactttgct caaacagctg ctcagaaaga ctatgattta tatcatggcg
    58861 gttggggacc tgattatcaa gacccgtcaa cctatctcga tatttttaat actaatagtg
    58921 gaggagttct acaaaatctt ggactagagc ctggagaagc caatgacaag gctaaggaag
    58981 ttggactgga tgtctatact caaatgttgg aagaagctaa taaagagcaa gatccagcca
    59041 aacgttatga gaaatatgct gatattcaag cttggttgat tgatagttct ttagttcttc
    59101 caagtgtttc gcgtggggga acaccatcat tgagaagaac cgtaccattt gctgctgcct
    59161 atggtttaac cggtacaaaa ggggttgaat catataaata cctcaaagta caagataaga
    59221 ttgtcacaac agacgaatat gcaaaagcca gagaaaaatg gttgaaagaa aaagaagaat
    59281 ccaataaaaa agcccaagaa gaattggcaa aacatgtcaa ataaaatcta attgtagata
    59341 agtttgtgta agaagtcttt gtgcttaaaa acgcataata tcaggtatat aaaatcctag
    59401 atgttatgcg ttttgttata ggaagattta ttagatttta tcctcaaatt gagttattcc
    59461 cgactttatc cgttgaaata ttataaggag tatttatgaa attaaaatta ttaagagtag
    59521 atactaaggt gattatgggg agtttcttac ttgttctgtc tagtctactt gctttgttgc
    59581 ttccccttat cttaaagggt ttaatagatg ggagttctat tgaaaatata ggttccaaag
    59641 tatttcaatc gtttttgatt tttattggtc aagccttgtt ttcttctatt ggttactatc
    59701 tgtttagtca atcgggtgaa aaaaagatag caaaaatcag gaaaaaagtg atagaggggt
    59761 tgatttatgc agagaaatac ttctttgata agagccaaag tggggagttg acttctgcca
    59821 ttgtcaatga cacgagtgtc attcgtgagt ttttaattac gactttccca aatattattc
    59881 tgagtttagt tatggtactt ggttccattg tagtcttatt tagtcttgat tggaatcttt
    59941 ctctactttt attcatcact cttccttgta tgatgtttat tatcttgccc ctttccaata
    60001 tcagtgaaaa gtatagtcgt cgtttacagg aggaaatcgg atttttaaca gggcaattga
    60061 ctgaaaagat tcaagaacat gaattaatta agactaacca agcagagaaa agtgtacagg
    60121 atgttttgga taattgtatt gaaagggtac aaaacaattc actgaaatca gatagggtta
    60181 cttcttttga gaccccgttt gctctcttat ttatctttgc gactatagtt gtgatgctaa
    60241 cctatggggg ttatcgggtc agcgcaggat atatatctgt gggaaccttg gtttcgtttt
    60301 tgatttacct ctttcaatta cttaatccta ttagtaatat agctaatttt gtaactattt
    60361 attctagtag caagggatct tcagttgcac tggagaactt gcttgcagtt cctaaagaaa
    60421 aatttgaggg aggaaaatcg gtatcaggac aagggttgaa ttttaaccat gtctattttg
    60481 gttatgatga aaatcgacct gtcttaaagg atattacttg ttcaattttc aaggggcaaa
    60541 aaattgcttt tgttgggcca tctggatcag gaaaatcaac gattgtgcgt ttattagagc
    60601 ggttttataa accgctttca ggagatattc taatggagca atcaagtata tatgatttta
    60661 acttaaaaga atggagaagt aaaatcgctt gggtttcaca aaataatgca gtcttatctg
    60721 gcagtattcg tgacaatctt tgtctcggtt tgaatcgctt agtaactgat gatgaattga
    60781 tgaaagtgct agacttagta tcactaggtg atgagattcg ctccatgaaa gagggactag
    60841 atactgaagt tggtgaacgc ggacgactct tgtcaggggg gcgaagccaa agacttcaaa
    60901 tagctagagc ctacttaaaa gatgctgaaa ttcttatatt tgatgaagcc actgctaatc
    60961 ttgatgcgga ttctgagtat gcgattatca gtagcctcta ttctgtacta aaggagaaga
    61021 cggttgtgat tatagcgcat cgcttgtcaa cggtaaaaga tgtggattgt attttcttct
    61081 tagaggaggg gaaaatcact ggctcaggaa ctcataagga actactggaa aatcatgagc
    61141 gttatgctcg ttttgtgcag gagcaaatga tagagtgaag gtgtcttttg agattcacca
    61201 ttttatagtc tattaaaggg agcaggaaaa actccctttt tatatagttt gaaactataa
    61261 ctagctcttg aaaagaagaa aatgagttga tgaaaataag tggtacaata gttactatag
    61321 atttggagga attgtatgag caaggaatta cacattaaca caattttagc acaagcaggg
    61381 atcaagtctg atgaggcaac aggtgctttg gtgacaccac ttcatttttc aacgacctat
    61441 cagcatccag agtttggtcg atctactggg tttgactata cgcgcactaa aaatccaact
    61501 cgtagtaagg ctgaggaagt cttggcggct attgagtcag cagactatgc cctagcgact
    61561 agctcaggga tgtcagctat tgtactggcc tttagcgtct ttccagtagg aagtaaggtc
    61621 ttggcagtgc gtgatcttta cggtggttct tttcgctggt ttaaccaagt ggagcaggaa
    61681 ggtcatttcc attttaccta tgccaataca gaagaagagt tgattgccga gttagaaaag
    61741 gatgtggatg ttctctatat cgaaacccca accaatccct tgatgttgga atttgatatc
    61801 gaaaaactag caaaattggc tcatgctaag ggtgccaaag tggtggtgga caataccttc
    61861 tatagcccta tctaccaacg tccgattgaa gatggagcag atatcgttct ccattcagca
    61921 accaagtatc tagcaggcca caatgatgtc ttggctggag tggttgtgac caatagttta
    61981 gaactatacg agaagctttt ttacaatctt aatacaacag gggcagtctt gtctccattt
    62041 gacagctacc agttgcttcg tggtctcaag accttgtctc ttcgtatgga gcgctcaaca
    62101 gctaacgccc aagaagtggt tgcctttttg aaggattctc cagcagttaa ggaagttctc
    62161 tacactggtc gtggaggcat gatttccttt aaagtagccg atgaaacacg cattcctcat
    62221 attttgaaca gtctcaaggt cttctctttt gcggaaagtt tgggtggagt ggaaagtctt
    62281 attacttatc caacgactca aactcatgct gatattccag cagaagtacg ccattcttat
    62341 ggtttgacag atgacctctt gcgtttgtct attgggattg aggatgctag agatttgatt
    62401 gcagatttgc gccaagcctt agaaggataa gacaaagatg ggaaaatatg attttacaag
    62461 cctgcccaac cgtttagggc accataccta taaatggaaa gaaacagaaa cggatagtga
    62521 agttctacca gcttggatag cggatatgga ctttgtagtc ttgcctgaaa tccaccaagc
    62581 cgtgcaaact tacgcagacc aactggttta tggttatacc tatgccagtg aagacttaat
    62641 taaggaagtt caaaagtggg aagctacaca atacggttac aactttgaca aagaggctct
    62701 tgtctttatc gagggtgtgg taccagccat ctcaacagct attcaaacct ttacaaaaga
    62761 aggcgaggcg gttttaatta acacgcctgt ctacccaccc tttgctcgca gtgtcaagtt
    62821 gaataatcgt agattgatta ctaattcctt agtggaaaag gatagtctgt ttgagattga
    62881 ctttgaccaa cttgaaaagg atttggtgga agaggaggtt aaactctatg ttctttgcaa
    62941 ccctcacaat cctggtggac gtgtttggga aaaagaagtg ttggagaaga ttggccaact
    63001 ctgccaaaaa cacggtgttt tgttagtttc ggatgagatt caccaagatt tgaccctctt
    63061 tggtcacaaa caccagtctt tcaataccat caatcctgcc ttcaaaaatt ttgctatcgt
    63121 cttgagcagt gccactaaaa catttaatat tgctggaaca aaaaattcct atgcagtcat
    63181 tgaaaatcct aagttgagac tggctttcca gaaacgcctg ttggccaata atcagcatga
    63241 aatttcaggc ttgggttatt tggcgacaga agctgcctat cgatacggta aagattggct
    63301 agaggaactc aagcaagtct ttgaagacca catcaattat gtggtggatc tatttggaaa
    63361 agagactaaa atcaaggtca tgaaaccgca aggtacctac ttgatttggc ttgacttttc
    63421 agcttatgac ctgactgatg aaacattgca agagttgttg agaaatgaag ccaaggttat
    63481 cctcaaccgt ggtttggatt ttggagagga aggaagtctc catgcccgca tcaatatagc
    63541 tatgcccaaa tctctgttgc aagaagtctg tcagcggatt gtggctactt ttgccaaatg
    63601 ttaaaaatcc agccttctag gagaaaagtc ttcctagaag gctattttca taggcgaaaa
    63661 tatggtataa taaaaagata aggtaaaggt gaaaatatgg ctaaattgat tccggggaaa
    63721 attcgtatcg aaggtgttgc cctttatgaa actggtaagg ttgatatcat caaagaaaag
    63781 aacaatcggc tctacgctcg cgttgcagaa gaagaactgc gctatagttt agaggatgat
    63841 ttgatttttt gtgcctgtga tttttttcaa aagaggggct actgtgtgca tttggcagcg
    63901 ctagagcatt ttctgaaaaa tgatgagcgt ggtcaggaaa tcttgtggag tctggaagaa
    63961 ggtcatgaag aaaaagaggc cgttgaaacc aaggtgacct tgggtggcaa gtttttggat
    64021 cgaattttat ctccaaaatc agaatgcgcc tatgagttat cagctgtggg tcaggtggaa
    64081 gcaggaacca atcatatctt gtggactctg agaatcggcc aaatcaatag ccaaaaatac
    64141 tatgtcattc gggatattcc acttttttta aggattgtcg agcagagaaa gtcgtatatg
    64201 attggcaaga tttatgagga gtctctttct tgggaagcct ttgatgatgc cagccaagaa
    64261 cttttgactt tcttgcacgg actaacggag gaagggctgg ttccagacct ctttttccaa
    64321 aaccaaggtc ggcacctctt tttccctctg accttttttg agcagggtgt gaatttactg
    64381 atggccttgc cccattttca atttgaccat caagtggata gctaccaaac cctgcttttt
    64441 caggatatgc atgctgatgc caatctattt gcctttacag taacagaata ttcggactat
    64501 tttgaaatgg aaatcagtga gagtcctagg gtcaatgtct tttatcaggg agcagtactt
    64561 ttccataaag gtcaggttta ttttttaaca gaccagcaga tgcgtcttct caaggaaatt
    64621 aaagcgttac ctgtgggtca acaaggaaag aaatacctgc aatttgatag cagtgatcga
    64681 gacaaattgg cctcctgtct gactcttttt atccagatgg gcaccgtttc agctccagaa
    64741 cgtctacaaa tcaaaacttt tgcgccctct ttttactttg acagggaaga agataatcgg
    64801 attcgtttag aaattcagtt tgattatgga aatagacagg tatctagccg acaagagcta
    64861 gaagaattac ccttttcaag tgacgcagac ttagaagaaa gaattttcca agtctgtttg
    64921 gctgcgggct ttgaagccga ttttcaatct tggcgtcagg ccttaaaagc ggagtctgtc
    64981 tatcattttt ttcatgatat cattccagtc tttgaaaaac tcgggcatgt tgacctatca
    65041 gataaactag aagagcttta tagcctagcg agtcctcagg tgcagattgc ctccaaggga
    65101 ggtctcttgg aaatccagtt tgattttcaa gatattgtcc aggaggaaat cgaccaagcc
    65161 atacaggcct tggttgccaa tcaggatttt tatattgatt cgtctaatca agtttacttt
    65221 ttcgatgaag aaaccaagaa aattcgccaa aatctacagg aactggggca atttgaatta
    65281 aaagatggga ccttgcaggc tcggaaatcc ttagcctaca gtttagctca tctctttgaa
    65341 gggcgagacc gtgtttcttt ttcacaagaa ttccagaatc tggcccagga tttgacacat
    65401 ccagaggact ttcctttgca agcaactcag gtcaaggctg acttgcgaga ttatcaggaa
    65461 aagggaattg gctggttgca gatgctccat cattatggtt ttggtgggat tttggctgat
    65521 gatatgggac ttgggaaaac ccttcagacc attgcttttt tgactagtca agtgacaaaa
    65581 gaaagtcgag tcttgattct cgctccgtcg ggtttgattt acaactgggc ggatgagttt
    65641 cagaaatttg ctccacagtt ggatgtggct gttgttcatg gtttgaaagc aagtcgtgag
    65701 gagattcttg ccgataacca tcaaatctat gtgactagct atgccacctt ccgtcaggac
    65761 agtgagcttt atcaggggat ggcctttgac ttccttttct tagatgaagc tcaggtcatg
    65821 aaaaatgccc agaccaagat tgcccagacc ttgagacaat ttgtggtgcc gtcggtcttt
    65881 gccttgtcag gaactccgat tgaaaaccat ctgggtgagt tgtggtctat tttccaaatc
    65941 gtcatgcccg gacttttgcc aagcaagaaa gaatttatga aattgccagc agagcgagtg
    66001 gctcagttta tcaagccttt cgtgatgcga cgcaagaaag aagaagttct gactgaattg
    66061 ccagacttga ttgaggtggt ttataaaaat gaactggaag accagcaaaa ggctatttac
    66121 cttgcccagt tacaacagat gcgagaccat ctggctcaag tgtcagaaca ggaatttcag
    66181 cgaagtcgtg tggaaatttt atctggtttg atgcgcttgc gtcaaatatg tgacactcct
    66241 gccctgttta tggaagatta tcagggagcc agcggcaaac tagatagtct ccgagaccta
    66301 ctggtacagg tggcagacgg cggacaccgt gtcttgattt tctctcagtt caagggaatg
    66361 ttggaaaaaa ttgaacaaga actgccagac ttgggcctga cttcctttaa aattacgggt
    66421 tcaaccccag ccaaggaaag acaagacatg accaaggcct ttaaccaagg agaaagagat
    66481 gcctttctga tttccctcaa ggctggtggg gtcggtctga acctgacagg tgcagataca
    66541 gtgatcttgg ttgacctttg gtggaatcct gcggtggaag cgcaagccat tggccgtgcc
    66601 catcggatgg gtcaggaaga aacggttgag gtctatcgct tggtgaccaa gggaaccatt
    66661 gaagaaaaaa ttcaggaact ccaagaacaa aagaaacatc tggtgtcaca agtattggac
    66721 ggcacagagt cacgtggtag tctgacccta gcagaaatta gagaaatttt gggaatttct
    66781 gaagccagca cttgaaaaat caagacaata cgctataatg aagtgttgaa aggaaataga
    66841 aaatgaaaca agaacgattt ccattggtgt cagatgacga ggtcatgttg actgaaatgc
    66901 cagtcatgaa tctctatgat gagtctgatc tgatcagtaa tatcaagggt gagtatcgag
    66961 ataaaaatta tttagaatgg gctcctattg ctgaagaaaa accagtaaaa ccgattgaaa
    67021 agcaagtagg gaaacctaaa aaagctcctt taggggttaa aaaagaagga aagagctatg
    67081 cggaggtggc gcgtgaagaa gcgcgtgcag acttgaaaaa gaaacgctct gctaactacc
    67141 taactcagga tttcagtctt gcgagacgtc attctcagcc cagtctagtt agacagggca
    67201 atcaaccgac agctcctttc caaaaggaaa atcctggtga atttgtcaaa tatagccaaa
    67261 aattgaccca gtctcattat atcttggcgg aagaagttca ttctatccct accaagaatg
    67321 aagaagtgtc agcacctgct ccaaagaaaa acaattatga ttttctaaag aagagccaaa
    67381 tctacaataa aaaaagtaaa caaacagaac aagaacgtcg ggttgcccaa gagttgaatc
    67441 tgaccagaat gacagaatag gggagaaaac atgtcaaaga catatcattt tatcggaatt
    67501 aagggatcag ggatgagtgc cttggccttg atgttgtacc agatggggca caaggttcag
    67561 ggatcagatg ttgaaaagta ctactttacc caacgcggtc ttgagcaggc aggaattacc
    67621 attcttcctt ttgatgaaaa gaatctagac ggtgatatgg aaattatcgc tggaaatgcc
    67681 tttcgtccag ataacaacgt cgaaattgcc tatgcggacc aaaatggtat cagctacaaa
    67741 cgttaccatg agtttctagg tagctttatg cgtgactttg ttagcatggg agtagcagga
    67801 gcacatggaa aaacttcaac gacaggtatg ttgtctcatg tcttgtctca cattacagat
    67861 accagcttct tgattggaga tgggacaggt cgtggttcgg ccaatgccaa atattttgtc
    67921 tttgaatctg acgaatatga gcgtcacttc atgccttacc acccagaata ctctattatc
    67981 accaacattg actttgacca tccagattat ttcacaagtc tcgaggatgt tttcaatgcc
    68041 tttaacgact atgccaaaca aatcaccaag ggtctttttg tctatggtga agatgctgaa
    68101 ttgcgtaaga ttacgtctga tacaccaatt tattattatg gttttgaagc tgaaggcaat
    68161 gactttgtag ctagtgatct tcttcgttca acaactggtt caaccttcac cgttcatttc
    68221 cgtggacaaa acttggggca attccacatt ccaacctttg gtcgtcacaa tatcatgaat
    68281 gcgacagccg ttattggtct tctttacaca gcaggatttg atttgaactt ggtgcgtgag
    68341 cacttgaaaa cattttccgg tgttaaacgt cgtttcactg agaaaattgt caatgataca
    68401 gtgattatcg atgactttgc ccatcatcca acagaaatta ttgcgacctt ggatgcggct
    68461 cgtcagaaat acccaagcaa ggaaattgta gcagtctttc aaccgcatac ctttacaaga
    68521 accattgcct tgttggacga ctttgcccat gctttaaacc aagcagatgc tgtttatcta
    68581 gcgcaaattt atggctcggc tcgtgaagta gatcatggtg acgttaaggt agaagaccta
    68641 gccaacaaaa tcaacaaaaa acaccaagtg attactgttg aaaatgtttc tccactccta
    68701 gaccatgaca atgctgttta cgtctttatg ggagcaggag acatccaaac ctatgaatac
    68761 tcatttgagc gtctcttgtc taacttgaca agcaatgttc aataggatgt tcccatggaa
    68821 attccaatta agatcattca ggcaagcaag tctgatttgc ctgagatagg ggcacttcag
    68881 acctcgtctt ttccagctga aaagcagcaa ctttcccata ttttagaaga aagtatccgt
    68941 aagtgtgcgg atacctttct tctagctagg gatgaaaatc aacttttagg ctatatttta
    69001 tcaagtcccc agtcagacaa tccgcaatat ctaaaagtac attctttagt catcgagtct
    69061 gaccatcaga gacagggctt gggaacactt cttcttgcag ccttgaaaga ggtggcagtt
    69121 gagctggatt acaaagggat tcgtttggag agtcctgatg agctgctttc ctattttgaa
    69181 atgaacggtt ttgttgatga agaagcaact ttgctctatg caactagcca gggctatagt
    69241 atgatttggt ttaatccctt ttatctggag gaacaatgaa aatcagacaa gcaagattag
    69301 aagatttgga tcggattgtt gaacttgaat ttgaaaattt ttcggtcgaa gaagccattc
    69361 ctccttctgt ctttgaagca catttgagag aaattcagac ctcttttctg gttgctgaaa
    69421 aagaaggaag aatcatgggt tatatcgaag gaccagttgg cctgcaccgc catctgcaag
    69481 accagtcttt tacagaagaa ataaaagact atagtcatga gcctggtggt tatatctctg
    69541 tgacctgtct gtctattgcc aaggaggcac agggactcgg actgggtcag aaattgctga
    69601 cagccttgaa agaagttgct cttgaagatg agagagatgg cattaatcta acctgtcatg
    69661 actatctcat cgcctattat gaaaaacatg gatttgtcaa cgaaggccag tcccagtcaa
    69721 cctttgcagg ggaaacatgg tatgatatgg tctgggaaat gaaaaaataa gttaggaaaa
    69781 gtatcataga ttgctttttt tggactttta agatataata ttatggatta tcaacaagga
    69841 ggaaaaactt ttgagtgaaa agtcaagaga agaagagaaa ttaagcttta aagagcagat
    69901 tctgagagat ttagaaaaag taaaaggcta tgatgaagtt ctgaaagaag atgaggcagt
    69961 agttcgcact cctgcaaatg aaccttcagc tgaagaactc atggctgatt ccttgtcaac
    70021 ggtagaggag attatgagaa aagctcctac cgtgcctact cacccaagtc aaggtgtacc
    70081 agcttctcca gcagatgaga ttcaaagaga aactcctggt gttccaagtc atccaagtca
    70141 agatgtacct tcttctccag cggaagaaag tggatcaaga ccaggtccag gtcctgttag
    70201 acctaagaaa cttgaaagag aatacaatga aaccccaaca agggtagctg tttcctatac
    70261 gacggcagag aaaaaagcag aacaagcagg tccagaaaca cctacgcctg ctacagaaac
    70321 agtggatatc atcagagata catcacgtcg tagccgtaga gaaggagcaa aacccgttaa
    70381 gcctaagaaa gagaagaagt cacatgtgaa agcttttgtg atttcattcc ttgtattcct
    70441 tgccttgctc tcagcaggtg gttactttgg ttaccagtac gtgctagatt ccttattacc
    70501 tatcgatgct aattctaaga aatatgtgac ggttggaatt ccagaaggtt caaacgttca
    70561 agaaatcggt acgacgcttg aaaaagctgg tttggtaaag catggtctga tttttagttt
    70621 ttatgccaag tataaaaatt ataccgactt gaaagcaggt tactacaatt tgcaaaagag
    70681 tatgagtaca gaagacttac tcaaagagtt gcaaaaaggt ggaacagatg aaccgcaaga
    70741 acctgtactt gcgactttga caattccaga aggttatacc ttggatcaga ttgctcaaac
    70801 tgtgggtcaa ttgcaaggtg acttcaaaga gtctttgaca gcggaggctt tcttggctaa
    70861 agttcaagat gagacgttta tcagtcaagc agtagcgaaa tatcctactt tactggaaag
    70921 tttgcctgta aaagacagcg gtgcgcgtta tcgtttggaa ggataccttt tcccagctac
    70981 atactctatc aaggaaagca caactattga gagcttgatt gatgagatgt tagctgctat
    71041 ggataagaac ctatctcctt actatagtac tatcaaatct aaaaacttga ctgtcaatga
    71101 gttgttgacc attgcttcct tggtcgaaaa agaaggtgcc aagacagaag atcgtaagct
    71161 cattgcaggt gtattttaca atcgtttgaa tcgtgatatg ccacttcaaa gtaatattgc
    71221 aatcttgtat gcccaaggaa aactggggca aaatatcagt ctagctgagg atgttgcgat
    71281 tgataccaac attgattcac cttataatgt ttataaaaat gtaggtctca tgcctggtcc
    71341 agtcgatagt ccaagtctgg atgcgattga gtcaagcatc aatcaaacta agagcgataa
    71401 cctctacttt gtagcagatg tcacagaagg caaggtctac tatgctaaca atcaagaaga
    71461 ccacgaccgc aatgtcgctg aacatgtcaa cagcaaatta aactaaacaa actaaaatta
    71521 tgtgatactt cacataattt tctttagaaa atattatcag aagaaagttg agaaaaatgg
    71581 cagaaaaaac atatcctatg acccttgagg aaaaggagaa acttgaaaaa gaattagaag
    71641 aattgaaatt ggttcgtcga ccagaagtgg tagaacgcat taagattgcc cgttcatacg
    71701 gtgatctttc agaaaacagt gagtacgaag cagctaagga tgaacaagcc tttgtcgaag
    71761 gacaaatctc tagcttagaa acaaaaatcc gctatgctga aatcgtcaat agcgacgcag
    71821 ttgcccagga cgaagtagcg attggtaaaa cagtcaccat ccaagaaatt ggtgaggacg
    71881 aagaagaagt ttatattatc gtaggttcag ctggtgcgga tgcctttgca ggtaaggttt
    71941 caaatgaaag cccaattggg caggccttga ttggcaagaa aacaggtgat acagcaacca
    72001 ttgaaacgcc tgttggtagc tatgatgtaa aaatcttgaa ggttgaaaaa acagcctaaa
    72061 aacagaaaaa ggagtgggga ggcgatgtgc ttcactcact cctttttcca ttttgctact
    72121 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgcc gtatgtatgg ttactgactt
    72181 cgtcagtttc atctacaacc tcaaaacagt gttttgagca acctgcggct
//
LOCUS       DAVKZB010000002        66364 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKZB010000002 DAVKZB010000000
VERSION     DAVKZB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.002"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            10..2040
                     /locus_tag="VXA80_000068"
     CDS             10..2040
                     /locus_tag="VXA80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0352639.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            2044..2328
                     /locus_tag="VXA80_000069"
     CDS             2044..2328
                     /locus_tag="VXA80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0352640.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            2341..3687
                     /locus_tag="VXA80_000070"
     CDS             2341..3687
                     /locus_tag="VXA80_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0352641.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            3691..4548
                     /locus_tag="VXA80_000071"
     CDS             3691..4548
                     /locus_tag="VXA80_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0352642.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            4545..5477
                     /locus_tag="VXA80_000072"
     CDS             4545..5477
                     /locus_tag="VXA80_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV0352643.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            5710..7413
                     /gene="ilvD"
                     /locus_tag="VXA80_000073"
     CDS             5710..7413
                     /gene="ilvD"
                     /locus_tag="VXA80_000073"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV0352644.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(7529..7609)
                     /locus_tag="VXA80_000074"
                     /pseudo
     CDS             complement(7529..7609)
                     /locus_tag="VXA80_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7667..8008)
                     /locus_tag="VXA80_000075"
     CDS             complement(7667..8008)
                     /locus_tag="VXA80_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0352645.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            8132..8983
                     /gene="rgg"
                     /locus_tag="VXA80_000076"
     CDS             8132..8983
                     /gene="rgg"
                     /locus_tag="VXA80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV0352646.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSTEY
                     YRKTLDS"
     gene            9174..10421
                     /locus_tag="VXA80_000077"
     CDS             9174..10421
                     /locus_tag="VXA80_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV0352647.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASVFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLDLSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANVFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLFIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            10499..11788
                     /locus_tag="VXA80_000078"
     CDS             10499..11788
                     /locus_tag="VXA80_000078"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV0352648.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLEIISKNFSEIFEKLGFYTQ"
     gene            11785..11862
                     /locus_tag="VXA80_000079"
                     /pseudo
     CDS             11785..11862
                     /locus_tag="VXA80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            12129..12443
                     /locus_tag="VXA80_000080"
     CDS             12129..12443
                     /locus_tag="VXA80_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352649.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            12453..12659
                     /locus_tag="VXA80_000081"
     CDS             12453..12659
                     /locus_tag="VXA80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0352650.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            12659..12856
                     /locus_tag="VXA80_000082"
     CDS             12659..12856
                     /locus_tag="VXA80_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352651.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            12912..13592
                     /locus_tag="VXA80_000083"
     CDS             12912..13592
                     /locus_tag="VXA80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV0352652.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            13604..14245
                     /locus_tag="VXA80_000084"
     CDS             13604..14245
                     /locus_tag="VXA80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0352653.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            14263..14493
                     /locus_tag="VXA80_000085"
     CDS             14263..14493
                     /locus_tag="VXA80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352654.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            14529..14609
                     /locus_tag="VXA80_000086"
                     /pseudo
     CDS             14529..14609
                     /locus_tag="VXA80_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14733..14816
                     /locus_tag="VXA80_000087"
                     /pseudo
     CDS             14733..14816
                     /locus_tag="VXA80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            14935..16698
                     /gene="aspS"
                     /locus_tag="VXA80_000088"
     CDS             14935..16698
                     /gene="aspS"
                     /locus_tag="VXA80_000088"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV0352655.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVVAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            16676..17617
                     /locus_tag="VXA80_000089"
     CDS             16676..17617
                     /locus_tag="VXA80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0352656.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(17621..17812)
                     /locus_tag="VXA80_000090"
     CDS             complement(17621..17812)
                     /locus_tag="VXA80_000090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352657.1"
                     /translation="MKIKVQTRKLAAGCSKHYFEVVDRTDEVSSKHGFEVVDETDEVS
                     NHIYGKVKLTWFEEIFEEY"
     gene            complement(17954..18940)
                     /locus_tag="VXA80_000091"
     CDS             complement(17954..18940)
                     /locus_tag="VXA80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0352658.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(18950..19750)
                     /locus_tag="VXA80_000092"
     CDS             complement(18950..19750)
                     /locus_tag="VXA80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV0352659.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(20003..20845)
                     /locus_tag="VXA80_000093"
     CDS             complement(20003..20845)
                     /locus_tag="VXA80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0352660.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(20847..22139)
                     /locus_tag="VXA80_000094"
     CDS             complement(20847..22139)
                     /locus_tag="VXA80_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0352661.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(22250..23521)
                     /locus_tag="VXA80_000095"
     CDS             complement(22250..23521)
                     /locus_tag="VXA80_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0352662.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            24071..25588
                     /gene="malQ"
                     /locus_tag="VXA80_000096"
     CDS             24071..25588
                     /gene="malQ"
                     /locus_tag="VXA80_000096"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV0352663.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            25614..27872
                     /gene="glgP"
                     /locus_tag="VXA80_000097"
     CDS             25614..27872
                     /gene="glgP"
                     /locus_tag="VXA80_000097"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV0352664.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLRVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(28917..29489)
                     /locus_tag="VXA80_000098"
     CDS             complement(28917..29489)
                     /locus_tag="VXA80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352665.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(30112..30960)
                     /locus_tag="VXA80_000099"
     CDS             complement(30112..30960)
                     /locus_tag="VXA80_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0352666.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            31112..31390
                     /locus_tag="VXA80_000100"
     CDS             31112..31390
                     /locus_tag="VXA80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352667.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            31392..33455
                     /locus_tag="VXA80_000101"
     CDS             31392..33455
                     /locus_tag="VXA80_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0352668.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(33533..34789)
                     /locus_tag="VXA80_000102"
     CDS             complement(33533..34789)
                     /locus_tag="VXA80_000102"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV0352669.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGGRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            34931..37396
                     /locus_tag="VXA80_000103"
     CDS             34931..37396
                     /locus_tag="VXA80_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0352670.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            37461..38372
                     /locus_tag="VXA80_000104"
     CDS             37461..38372
                     /locus_tag="VXA80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0352671.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIVLIILPMIFLSIEEAKESR"
     gene            38602..39300
                     /gene="dapD"
                     /locus_tag="VXA80_000105"
     CDS             38602..39300
                     /gene="dapD"
                     /locus_tag="VXA80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV0352672.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            39368..40498
                     /locus_tag="VXA80_000106"
     CDS             39368..40498
                     /locus_tag="VXA80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV0352673.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            40510..41049
                     /locus_tag="VXA80_000107"
     CDS             40510..41049
                     /locus_tag="VXA80_000107"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV0352674.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKRVLTPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            41033..41710
                     /locus_tag="VXA80_000108"
     CDS             41033..41710
                     /locus_tag="VXA80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV0352675.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(41777..42676)
                     /gene="galU"
                     /locus_tag="VXA80_000109"
     CDS             complement(41777..42676)
                     /gene="galU"
                     /locus_tag="VXA80_000109"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV0352676.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(42698..43714)
                     /locus_tag="VXA80_000110"
     CDS             complement(42698..43714)
                     /locus_tag="VXA80_000110"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0352677.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            43926..44366
                     /locus_tag="VXA80_000111"
     CDS             43926..44366
                     /locus_tag="VXA80_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0352678.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(44489..44905)
                     /locus_tag="VXA80_000112"
     CDS             complement(44489..44905)
                     /locus_tag="VXA80_000112"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0352679.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(44973..45623)
                     /gene="phoU"
                     /locus_tag="VXA80_000113"
     CDS             complement(44973..45623)
                     /gene="phoU"
                     /locus_tag="VXA80_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0352680.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(45638..46390)
                     /gene="pstB"
                     /locus_tag="VXA80_000114"
     CDS             complement(45638..46390)
                     /gene="pstB"
                     /locus_tag="VXA80_000114"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0352681.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(46392..47207)
                     /gene="pstA"
                     /locus_tag="VXA80_000115"
     CDS             complement(46392..47207)
                     /gene="pstA"
                     /locus_tag="VXA80_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0352682.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(47200..48063)
                     /gene="pstC"
                     /locus_tag="VXA80_000116"
     CDS             complement(47200..48063)
                     /gene="pstC"
                     /locus_tag="VXA80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0352683.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(48181..49056)
                     /locus_tag="VXA80_000117"
     CDS             complement(48181..49056)
                     /locus_tag="VXA80_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV0352684.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(49223..50554)
                     /locus_tag="VXA80_000118"
     CDS             complement(49223..50554)
                     /locus_tag="VXA80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV0352685.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(50555..51262)
                     /locus_tag="VXA80_000119"
     CDS             complement(50555..51262)
                     /locus_tag="VXA80_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0352686.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(51346..51822)
                     /locus_tag="VXA80_000120"
     CDS             complement(51346..51822)
                     /locus_tag="VXA80_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV0352687.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEVVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            51829..52375
                     /locus_tag="VXA80_000121"
                     /pseudo
     CDS             51829..52375
                     /locus_tag="VXA80_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(52690..54381)
                     /locus_tag="VXA80_000122"
     CDS             complement(52690..54381)
                     /locus_tag="VXA80_000122"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV0352688.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            54517..54963
                     /gene="argR"
                     /locus_tag="VXA80_000123"
     CDS             54517..54963
                     /gene="argR"
                     /locus_tag="VXA80_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0352689.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            55014..57548
                     /gene="mutS"
                     /locus_tag="VXA80_000124"
     CDS             55014..57548
                     /gene="mutS"
                     /locus_tag="VXA80_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV0352690.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDLYNMTPMQVMNVLVELKQKL"
     gene            57695..59389
                     /gene="patA"
                     /locus_tag="VXA80_000125"
     CDS             57695..59389
                     /gene="patA"
                     /locus_tag="VXA80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV0352691.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(59719..59982)
                     /locus_tag="VXA80_000126"
                     /pseudo
     CDS             complement(59719..59982)
                     /locus_tag="VXA80_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            60180..61946
                     /gene="patB"
                     /locus_tag="VXA80_000127"
     CDS             60180..61946
                     /gene="patB"
                     /locus_tag="VXA80_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV0352692.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEILI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            62085..62774
                     /locus_tag="VXA80_000128"
     CDS             62085..62774
                     /locus_tag="VXA80_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0352693.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            62778..62957
                     /locus_tag="VXA80_000129"
     CDS             62778..62957
                     /locus_tag="VXA80_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352694.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            62944..64293
                     /locus_tag="VXA80_000130"
     CDS             62944..64293
                     /locus_tag="VXA80_000130"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV0352695.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            64418..65878
                     /locus_tag="VXA80_000131"
     CDS             64418..65878
                     /locus_tag="VXA80_000131"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV0352696.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAVGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            65892..66326
                     /locus_tag="VXA80_000132"
     CDS             65892..66326
                     /locus_tag="VXA80_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0352697.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT        19488 a        12320 c        14438 g        20118 t
ORIGIN      
        1 ggaagagaaa tgtttgatta tagagcacta gttattttga tgactttgat ggatcattgt
       61 gagctatcat tatatgaatt atctgttaag gtgagcttac ctataaagga agtcaaagaa
      121 ggaatagatt atttagtgcc ttatctagct aataaaggga tagtgctgga taaaaaacaa
      181 ggtcgctata gtttatcaaa tcgtacgaag cagtctttga cagatattat taagtcggat
      241 gaattggttt taccaaagtc gactcgctta gctttaatct atctttacac tttttgccga
      301 ttagatttta tatcgaataa tcattaccaa gactttttga aagtaagtaa gaatacaacc
      361 ttatctgata ttcaatcatt aagaaagatt atgttagata atgatttgga gctagggtac
      421 agtagagcaa aaggttatac tttacacggt tcagagtgga ataagcaccg cttagctttt
      481 caaatggtta gtgagttgct ggaatcctcc atagggattt ggggtttgga ttatgtttta
      541 tccagttggg ggtattcatt aacttatgat ttgattgatc aggtagttaa agattattat
      601 gagaagctac agctagtacc tattgttaat caattaaaag tttacctttt cggtttagta
      661 ttcattctgt gtaggtatca aagggatgtt gaaagagtat gtctttcaga gacattagta
      721 tctcctgtta ttcaagatat aaccactatt ttattggata cagtggttga tttgggaatt
      781 atagatacgg tgttttctga ggatgattat cgctatatca ccgttttatt atcaagttgc
      841 tttgaaggtg aagtagatgt tgctccggtt tactttaatc aattaacaga ggctattata
      901 agtagaatgg aagatatttc tttgttacat tttaaacaaa gggaaggatt gagagaaaat
      961 cttcgccgcc accttatacc ggcttactat agattaaagt tcggcctacc tagttcaaat
     1021 gaatatgtat tgcatgttaa agaacattat cctgatttat ttgaattagt caaagattct
     1081 ttgatgccct tgatggacgc tattgacaat cccatacctg atagtgaaac agcatatttt
     1141 gttatccatt tcggaggtta tttaaagaaa gcagatactt tgcctcaaaa atggtataaa
     1201 gcagcaatca tttgtcctaa tggtgttagt tcttcattga tgttaaaaga gaatctatta
     1261 gcattatttc ctcaaattga gtttatagga acctcaaaga ttgatgattt acaggtgaaa
     1321 gctagtagtg actatgatat ggttttttct accataaagg tggagacaga aaagccaaat
     1381 tatctagttt cggttatgat gactgaagag caagcaatac aactagtaga actagtgtta
     1441 aaagattttc cgaatttaga gtatggagat tttgagattg aacaaatcct gaatattgtc
     1501 aaaagatatg gaattattac acaagaattg gaattaagat tggcgttaaa gaattatctt
     1561 tatcaaaaaa atgatagaaa ggaaattgta ccagtgctag aacaacttat taccaaagaa
     1621 acctatcagg ttagttcgca aaaattagga tggaaggagg cgattcgttt agcagctaaa
     1681 ccgcttttgg atcaagataa gataactgag aactaccctg aggcaatgat tcaaaaagta
     1741 gaagagtttg gaccttttat taatttaggg aagggagtag caattcctca cgctcggcca
     1801 gatgaaggtg tgaatgaaat aggaatgtca atgttagttt tggaagaacc gatttattta
     1861 ttggataatc cagaacaaga ggtaagattg ttgatttgta ttgcagccat tgataatgag
     1921 agtcatttaa aggctttgtc acatttaaca acaattttaa gagataaaaa tcatgttcaa
     1981 actttaatat catcaaaaaa ctataatgat attaaaatga taattaaaca ggaggattag
     2041 ataatgttaa aaattggtac agcttgtggt tcaggattag gttcaagttt tatggtacag
     2101 atgaatattg aatctgtatt gagtgatttg aatgtttcgg atgtagaagt tgaacattat
     2161 gatttaggtg gagcagatcc aaatgcagct gatatttgga ttgttggtcg tgatctagct
     2221 gattcagcta gtcatcttgg agatgttcgt atcttaaata gtattattga tatggatgaa
     2281 ctacgagaat taattactaa actttgtgaa gaaaaaggac ttatatagga ggctagcgtc
     2341 atgatgaagt tcatattgga tattgttagt acaccagcta ttttagtagc tttaattgca
     2401 attttaggat tagttcttca gaagaagaaa ttacctgata ttattaaagg tggaattaag
     2461 acctttgttg gtttcttagt tgtatctggt ggtgcaggaa ttgtacaaaa ttctttaaat
     2521 ccatttggta ccatgtttga gcatgctttt catttatctg gcgttgtgcc gaataatgaa
     2581 gcaattgtag ctgtagcttt aacaacatat ggctcagcta ctgcaatgat tatgtttgca
     2641 ggaatggtgt tcaatatctt aatcgctcgt tttactcgat ttaaatatat ttttttaaca
     2701 gggcaccaca ctctatatat ggcatgtatg attgcggtca ttttatcagt tgctggcttt
     2761 actagcttgc ctctcatctt actaggagga ttagcactcg gtattattat gagtatttcc
     2821 ccagcatttg tgcaaaaata tatggttcaa ttaactggaa atgacaaggt ggctttaggt
     2881 catttcagtt ctttgggata ttggttgagt ggttttactg gtagccttat cggtgacaaa
     2941 tcaaaatcaa cagaggacat taaatttcca aagagtttag cttttttacg tgatagtact
     3001 gttagtatca ctttatctat ggcagttatt tacattattg tagctatctt tgcagggtca
     3061 gaatatatag aaaaagaaat cagtagtggt acaagtggtc tagtttatgc tttacaatta
     3121 gcaggtcaat ttgcagcagg ggtatttgtt attttagcag gtgttcgcct tattttgggc
     3181 gaaattgttc cagcctttaa aggtatttca gagcgtcttg tacctaattc aaaacctgct
     3241 ttggattgtc cgattgttta tacttatgca cctaatgcag ttctaattgg atttatctct
     3301 agttttattg gtggtttagt aagtatggca attatgattg cttcaggaac ggttgttatc
     3361 ttaccaggcg ttgtgcctca tttcttctgt ggagcgactg caggtgttat tgggaatgca
     3421 tctggtggtg ttcgtggagc cactattgga gcatttttac aaggtatttt aatcagtttt
     3481 ctcccagtct ttttaatgcc agttttggga ggactcggtt tccaaggatc aactttctca
     3541 gatgcagatt ttggtctatc aggaattatt ttaggaatgt taaatcaatt tggctcacaa
     3601 gcaggcattg tgattggtct tgttcttatt ttagcagtta tgtttggagt atcctttatt
     3661 aaaaagccat ctgcaaagga ggaataaggg atgattttaa gtaaaaatag agaagatgag
     3721 ttaagaaaat ttgcgactaa catccgatta aatactctta gaacattgaa tcatcttgga
     3781 ttcggacatt acggaggaag tctgtctatc gtagaagttt tagcagtgct ttatggtgaa
     3841 ataatgccaa tgactccaga aatatttgca gcacgagata gagattattt catattatca
     3901 aaaggtcatg gaggaccagc tctatacagt acactctatt tgaatggttt ctttgacaaa
     3961 gaattcttat attctttaaa tacaaatgga accaaattac cgtctcatcc tgatagaaat
     4021 ctaacgccag gcatagatat gacaacgggc tctttaggac aaggaattag tgttgcaact
     4081 ggacttgcat atggtcagag aataagaaag agtccctttt atacttatgc tattgttgga
     4141 gatggtgagt taaatgaggg acaatgttgg gaggctatac agtttgcttc tcatcaacag
     4201 ttatccaact taattgtatt tgttgatgat aacaaaaaac aattagatgg ttttacaaag
     4261 gatatttgta atccaggtga tttcgtagaa aaattttcag catttggatt tgaatccatt
     4321 agggtcaagg gttcagatat tagagaaatt tatgaaggga ttgtccaatt aaaacagtca
     4381 aataattcat cacctaagtg cattgtatta gatactatta aaggtcaagg ggttcaagag
     4441 ctggaagaaa tgaaatccaa ccatcatctt cgccctactg tagaggagag acaaatgtta
     4501 acttcagttg tagaaagatt aagtcaggaa ttggaggaaa cagaatgatg agaagcacga
     4561 aagaattacg gcatgtatat agagacttcc ttttagaggc taatcaaagt tattctgata
     4621 tagtagtctt agaagctgat ttgtcaagtt cgatggctac tcataatctt gaaaaggact
     4681 ttggagaccg ttatgtgaat gttgggatta tggaagcaga aatggtcggc cttgcagcag
     4741 gcttgtctat tcaggggttt agaccttatc ttcatacatt tggtcctttt gcttcacgaa
     4801 gagtatttga tcaattattt atttctcttg gatacgcaca attggatgcc actgtgattg
     4861 gatcagatgc aggagtaact gcagaaatga atggtgggac acatatgcca tttgaagaaa
     4921 ttggattgtt acgtttaatt cctaaatcga tcatttttga agcaactgat gatatccaat
     4981 ttcgtgaaat attgaaccag acattagact taaaaggact aaaatatatt cgaacaatta
     5041 gaaaagctcc agttcctgtg tatcaaggtg gagaagattt ttctaaaggc tacattgagt
     5101 taaggcatgg tgaagatctt gtaatcgttg cttctggtat aatggttgct ccaagtattc
     5161 gagttgcaga tgaactgtct aaattaggtt attcagtaag tgtgatagat ttatttagaa
     5221 tcaaaccgat accagaacag ataaaaacaa tgttaagtgg aaaaactata tttactgtag
     5281 aaaaccacaa tcagataggt ggaattggca gttctttatg tgaattattc tctgatgatg
     5341 gaatgacaaa aattcatcgg atgggtgttc aagaaagatt cggtcaagta gggaaaatgg
     5401 attatcttct taatgagtat ggtttgagtg aatcgaatat aaaagagaag gttctagaaa
     5461 tttataatgc aagatagatg ttaaatacac ttatctgaaa gaatttctgt aaaaattaca
     5521 gtacgttatt taaagaaagc ggattaaaaa gcatattctg gtcatcagaa agtgcactaa
     5581 tccgctttgt tgtttttaat tttaaatatt tcacatgcgt atagaatttt ctgaaaattc
     5641 aagaatttta ttctgttcat tgcctttaaa ttgtgctata atagtaaaaa ctgaaatggg
     5701 agggataaga tgactgaatt agataaacgt caccgcagta gcatctatga cagcatggtt
     5761 aaatcaccta accgtgctat gcttcgtgcg actggtatga cagataagga ctttgaaaca
     5821 tcgattgtgg gagtgatttc gacttgggcg gaaaatacac catgtaacat tcacttgcat
     5881 gatttcggga aactggctaa agaaggtgtc aaatctgcag gcgcttggcc tgtacagttt
     5941 ggaaccatta ccgtagcgga cgggattgct atgggaacgc ctggtatgcg tttctctcta
     6001 acatctcgtg acatcatcgc ggactccatc gaggcggcta tgagtggtca caacgtggat
     6061 gccttcgtcg ctatcggtgg ctgtgacaag aacatgcctg gatctatgat tgctattgct
     6121 aatatggata tcccagctat tttcgcctat ggtggaacta ttgcaccggg aaatcttgat
     6181 ggtaaagata tcgacttggt ttctgtcttt gaaggtatcg gaaaatggaa ccacggtgat
     6241 atgacagctg aggacgtgaa acgtcttgaa tgtaatgcct gccctggccc tggtggttgt
     6301 ggtggtatgt atactgctaa taccatggca actgctatcg aagttctagg gatgagtttg
     6361 ccagggtcat cctctcaccc agctgaatca gctgataaga aagaagatat cgaagcagca
     6421 ggacgtgctg ttgttaagat gttggagctt ggtctcaaac catcagatat cttgactcgt
     6481 gaagcctttg aagatgctat cactgtaacg atggctctcg gtggttctac aaacgccact
     6541 cttcacttgc tcgccattgc ccatgccgca aatgttgact tgtcacttga ggacttcaat
     6601 acgattcaag aacgtgtgcc tcacttggcc gacttgaaac catctggtca gtatgtcttc
     6661 caagacctct acgaagtcgg tggtgtccct gcggttatga agtacttgtt ggcaaatggt
     6721 ttccttcacg gagatcgcat cacatgtact ggtaagactg tagctgaaaa cttggctgac
     6781 tttgcagact tgactccagg ccaaaaagtt atcatgccac ttgaaaatcc aaaacgtgcg
     6841 gatggtccgc ttatcatctt gaacgggaac cttgctcctg acggtgcagt tgccaaggta
     6901 tcaggtgtta aagtgcgtcg tcacgttggg ccagctaagg tctttgactc agaagaagat
     6961 gcgattcagg ccgttctgac agatgaaatc gttgatggcg atgtagtcgt tgttcgtttt
     7021 gttggaccta aaggtggtcc tggtatgcct gagatgctgt cactttcttc aatgattgtt
     7081 ggtaaaggtc agggagataa ggtggccctc ttgacggacg gacgtttctc tggtggtact
     7141 tatggtctgg ttgttggaca tatcgctcct gaagctcagg atggtggacc aattgcctat
     7201 ctccgtaccg gcgatatcgt tacggttgac caagatacca aagaaatttc catggccgta
     7261 tccgaagaag aacttgaaaa acgcaaggca gaaacaacct tgccaccact ttacagccgt
     7321 ggtgtcctcg gtaaatatgc ccatatcgta tcatctgctt cacgcggagc cgtgacagac
     7381 ttctggaata tggacaagtc aggtaaaaaa taaactcata ctcgaaaatc tcttcaaatc
     7441 acgtcaacgt cgccttgccg taggtatggt tactgacttc gtcagttcta tctacaacct
     7501 caaaacagtg ttttgagctg acttcgtcag ttctatccat aacttcaaaa cagtgttttg
     7561 agcaacctgc ggctagtttt ctagtttgcc ctttgatttt cattgagtat caaaaagcaa
     7621 gccattttcg gcttgctttt ttcatcttta aaagtgattg gcctgcttaa cgaggtggta
     7681 gtccaagggc aatacggcca tagcgactga ttcgtgtgat tttccaagca ggcgaccagg
     7741 taacttcaac cttgatatct ttgataccct cgatttgttt cagacctgcc acgatttcaa
     7801 taggcaggct ttcggcgcaa tcacaggcag tatcggtgaa ggtcatgaca atcttgcaga
     7861 gccccgtttc atccagattg atttcataaa tcagacctag attgtaaaca tccaattcca
     7921 catctgtatc aaaaactttc tctagttttt cgataatttg gtcttgcaag gccaaagcac
     7981 ggtcattgat tttgatatcg tctctcatag cagtccctcc acgtgataaa tatcctctat
     8041 tttctcataa ttctgacaat ttggcaagtt tttgatgaat tttctgaaaa atatgataaa
     8101 atgaagaaaa tacggaatca aggggaagtc tatggagcag attggaaaag tctttagaca
     8161 attacgagaa tcaagaaata tctcactgag acaagcaacc ggaggacaat tttcgccgtc
     8221 tatgttgtcc cgttttgaaa caggtcagag tgagctttcg gtggaaaagt ttctatttgc
     8281 tctggaaaat atatctgcca gtgtggagga gatcctcttt ctggcgagag gtttccagta
     8341 tgatacagat tctgagttga gaaaagaaat tacagatgtc ttggaaccaa agaatatagc
     8401 acctcttgag gacttatatc gcagggagta tcaaaagcat gcccattctc acaacaaaca
     8461 gaaacatatt ctaaatgcca ttattatcaa gtcttatatg aagagcatag atgaaagggt
     8521 agagttaaca gcagaggaag gaaaagtcct tcatgattat ttgttttcta ccgagatctg
     8581 gggaatctat gaactcaatt tattttcagt cagttctcca tttttatctg tttctctttt
     8641 tactagatat gtacgagaaa tggtacgtaa atctgatttt ctaatggaaa tgtctggcaa
     8701 tcgaaacctt tttcatacta tactattgaa tggcttttta gctagtattg agtgtgaaga
     8761 atttaccaat gcctattatt ttaaacgtgt tatcgaagag catttctaca aggaaaatga
     8821 gacctatttc cgaattgttt atctgtgggc agaaggtctc cttgatagca agcaaggtag
     8881 agtcaaggaa ggtcagaaaa agatggagga tgctgtccgt atttttgaga tgcttggctg
     8941 taataaatct accgaatact atagaaaaac tctcgattct tgaatatctg caaaccaaga
     9001 aaaaatttga agcgatagaa ctgttgccct ctctcaggtc attacaaaag ttctatcgtt
     9061 tttttgctca ttttgtttgt tgcatatatt caaaagaatc gtttcgcaaa ttcataagtg
     9121 ctatactaaa gctattcttg atgtagaact tagaacaagg agggagatta tctatgaaac
     9181 tattgtttag aaatccagct tatagaattt tgactttatc acgcttcttc aatgcttttg
     9241 gtgcttcggt tttcaacctg gtctttatcg tctatgcatc gaccttgtca caagcatctt
     9301 ttgctgttgc tatggcgaat attgtcatga ttcttccgac cttctttaca gtttttgcag
     9361 ggattcgggc agattacacg agggacaagg tcaaatggat ggtctatagc ggtttgtttc
     9421 aggcggttct attttttcta gcagccctag ttgttcagca agctagtctc tttgcctttt
     9481 ctagcctgtg tttgatcaat gtcattagtg atatcatcag tgattttgca ggtggcctgc
     9541 gtatgcctct tattaaggaa aaagtagctg aagatgattt gatggaggct tattcttttt
     9601 cacagttcat cacctatatt tcagctattg gtggtcaagc tttcggagtc tggctcttag
     9661 atctatcggt caacaatttt tctctcgttg cgggaatcaa tgcctgcttt ttcctagtat
     9721 cagccactat cctcttttta ggaaaaagca aattgagcct gtcaatgtca tctgctgatg
     9781 gtgaaaactt aaaaaatgag aagctttcta tcaaagacca gttcctaaca atttaccgaa
     9841 atttacgtct cgtttttctt aaaagtggac agaaaaactt tggttttatg ctctttgctg
     9901 tcttgcttat taattccttg ggtggcgctt tgggtggaat ttataatatc ttctttttga
     9961 gccattctct cttgaatttt tcttatacag aggcactatt tatcaatcaa ttctgtgttt
    10021 tagtagcaat catcatcagt agccttacgg gcaatgatta ttttgggaag cagtccttgc
    10081 ctagattgat gatgtgggcg accgtaggac ttagtctagt tggtttggct aacgtattca
    10141 atcaagtcgt gcttggtttg ctatttctct tctttactct gtatgtgtct ggtaaagttc
    10201 aaccaaagat tagtgccatg ctcatgaaaa atctagctcc agaggttcta gctcgtacta
    10261 gtaatttttt agatctattg tttaccttat tcatacctgt gggaacagct tgtttttcac
    10321 tagtagccgt atggaatata cagttgactt ggatgctatt tgttggtctt tccttgctag
    10381 ctattttttt gacaattctt aatctcaaaa atgatatcta aatcctaatt tttttcttac
    10441 tgttttaatc cctctattta tggtaaaata agactattaa gtttaaagag gattccctat
    10501 gaaattacaa aaaccaaaag gaacgcagga tattttacct gctgagtctg ctaagtggca
    10561 gtacgttgag ggctttgccc gtgagatttt caagcgctat aactatgcag aagtgcgcac
    10621 gcctattttt gagcattacg aggttatcag ccgctctgtc ggagatacaa cggatatcgt
    10681 aaccaaggaa atgtacgatt tttatgacaa gggtgaccgt catattaccc tccgtccaga
    10741 aggaactgcg cccgttgtcc gttcctatgt ggaaaataaa ctcttcgccc cagaagtgca
    10801 aaagccaagc aagttctact atatgggacc tatgttccgt tatgagcgtc cacaggcagg
    10861 gcgcttgcgc caattccacc agattggtgt tgagtgtttt ggctctagca atccagctac
    10921 cgatgtggaa acaatcgtta tggcagccca ttttttgaag gaaatcggta ttcaaggtgt
    10981 caaattgcac ctcaacactc ttggaaatcc tgagagccgt gcagcctacc gccaagcctt
    11041 gattgactat ttgacaccgc tcaaggagac cttgtctaag gatagccaac gtcgcttgga
    11101 ggaaaatcct cttcgtgtct tggactctaa ggaaaaagaa gacaaggtgg cagtagagaa
    11161 tgcgccgtct atcttggact ttcttgatga agaaagccaa gctcattttg atgctgtgcg
    11221 tcagatgttg gaaaatcttg gagtagatta catcatcgat accaatatgg tgcgtggtct
    11281 ggactactac aaccacacca ttttcgagtt tatcacagag attgagggca atgacttgac
    11341 agtctgtgcg ggtggtcgct acgatggttt ggttgcttac tttggaggcc ctgaaactgc
    11401 tggatttggt tttgggcttg gtgtagagcg tctgcttctc atccttgaaa aacaaggcgt
    11461 ggccctccct atcgaaaatg ccctagatgt ctatatcgca gtcttgggtg atggagcaaa
    11521 tgtcaaagct ctagaactag tccaagctct tcgccaacaa ggtttcaaag cagagcgtga
    11581 ttacctcaac cgtaagctca aagctcagtt caagtcagcc gatgtctttg tggctaagac
    11641 cctcatcacc ctaggagaga gtgaagtcga aagcggacaa gtgacggtca agaacaatca
    11701 aactcgagaa gaagtgcaag tgtcacttga gataatcagc aaaaatttct cagaaatctt
    11761 tgaaaaacta ggattttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
    11821 ggttgctcaa aacagtgttt tgaagttatg gatagaactg atgaagtcag ctcaaaacac
    11881 tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacctacgg caaggtgaag
    11941 ctgacgtggt ttgaagagat tttagaagag tattaatgat agataaagtg gtcaggaact
    12001 tactcctgac ctttttcttt tgcaaaatga caaaaatcat aaactttttg acaactatac
    12061 ttgtcaaaaa gaaaaagata tgttacaatg aattcaagtt aagaaataga aagcagaaag
    12121 gagttataat gtgggtatta ggatttatac tatttatgat tttcttttat tctaataatt
    12181 ctaaaaaaat caagaaacta gagaataaaa tcaaaaaact tgagcgaaaa gagaaaggaa
    12241 acgcagaaat gtcgagatta ttacaagaaa tgattggaaa ggaaccaatt ataacgggag
    12301 tgtatattgg gccagataac tgggaagttg tggatgttga tgaggaatgg gtcaaactac
    12361 gtagtgttga taatacggga aaagaaaaat tcaagttgca acgtattgaa gatatccaaa
    12421 ccgttgaatt tgacggagag taggtgtgta acgtgcaact aaaaaatcgt ctaaaagagc
    12481 ttcgagctcg cgatggtctc aatcaaaccg acctagccaa actggcaggg gtttccagac
    12541 agaccattag cctactagaa cgggatgagt acaccccatc cattatcatt gccctgaaaa
    12601 tatctcaaat tttcaacgaa acagtcgaat cggtatttcg tttggaggag gacgagtgat
    12661 gaacaagtat aaagtgattt attatgtagt ggtcatagct ttattagtca gcgtatttct
    12721 actgattggg atggacctaa gctggtttaa tccctatcaa agcgaccaat ttgtttgggt
    12781 ctactttgct ctcatcccag taactgaatg gattgaaaag aaatccaaaa atctagcaag
    12841 tgaaaaagga gaatgaatat gaaaaagaaa cgtagattgt tatttttgat gtctattgtc
    12901 ttgggggggt cttgggcatg tttgtaggga tgtttaaggc acgtgtcgaa tcccacgaaa
    12961 ttattttaga tgtaaaagcc ttgatgccat ggatatcagc tatttgttta ctgataggtt
    13021 tcattagtat gtttttgact ttcaatttct taaagaaaag cagaaaattt cattccttgt
    13081 atcaagagga aatggatgac gatctgaatg aaacctatta tgtgcaaatg tatcggaatc
    13141 ttgagtttgg aaccattgct tttaatatta caggtgtagc gattccattg gctattttta
    13201 tttcattaag tgaggtgatt atattgcata caaaccctca aacatttttc ctttctttct
    13261 tactctttgt ggtattctta gtcgctcaaa aatctctttt taaaaccatt gcgattgttc
    13321 gtcagtttga tttggaattt ttcgctacac caaaggatgt cttgaactat ataaattctt
    13381 atgatgaagg ggagcgtcag gctaatttgg aacagagttt tcgaatttta ttccaattac
    13441 accaatatgt cttaccagcc ttatatattt ttcttattat cctttctttc ttgacaggag
    13501 agattcagtt actagctttc ttgcttgtag gagccatcca tgtttatatc aatgtgatgc
    13561 agttacctat ggtaaaacgt tatttcaaat aaaggagtta tctatgaaat tacttaaaaa
    13621 ccttggctgg tttcttctag cccttctatc ctttttcttt atctatggct ttattcaggg
    13681 gctcgcgact gcgtcgcttg ctttaggcgc ttcaccctat gctgttactc tactctatgt
    13741 ggtcttggct ggagtatatg tgtacggcat ttacaaatgg tatcagaaag ctcctgttta
    13801 tattgagaag agcggcttta accgatttat ttggcttcct gccttggttt ggttcttatc
    13861 tctggtcgtt caattcttct tgccagatga tccttcagta aatcagcaaa tagcgacaga
    13921 cttgaccttg tctcaaccac ttttctcatt ctttgctgtg gttatttttg ctcctttgac
    13981 ggaagagatt gtttttagag ggatgttagc acgctatctc tttcctaagc aggacaatag
    14041 taaacgaacc ctgatttttc ttctggtatc tagtcttcta tttgccttga ttcattttcc
    14101 aggtgatgtg caacaatttt ttgtctattt tagccttggt tttagtttgg gtttggctta
    14161 cattagcaga aaaggtctgg tctacagtat ttctcttcac gctttgaata atttagtcgg
    14221 ctttttgatg attctcatgc tataatagag tcaggaggtc acatgaaacg agtaatttta
    14281 ttagcagtga ttcaggcagt cgttctattt ttcatcattg gagcgctagc ttatgccttc
    14341 aaaggcgatt tcttctataa ctatctagca gttgtctttg ctcctattgc aggtgtactg
    14401 cgttttggga cggcttacat aacggaaatt gtcttgcctc gaaaggcagc cgaaatcgct
    14461 gaaaagcgta aagcaggcaa aaattcaaaa taaaagagtc cggtgaagtt catcggattt
    14521 ttgtattata ctcaatgaaa atcaaagagc aaactaggaa gctaaccgca ggttgctcaa
    14581 aacactgttt tgaggttgtg gatgaaactg acgaagtcag ctcaaaacat ggttttgagg
    14641 ttgtagatga aactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
    14701 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagtaacc
    14761 atacctacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagcgct
    14821 aatttttagg gctttcacct gacttttaaa gactggcaaa cttttctgat gattttcatg
    14881 aagagcctgc gcttttatgg taaaatagta acagaataaa agaggagaga aataatgaaa
    14941 cgtagtatgt atgctggtcg tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa
    15001 ggatgggttg ggcgtcgtcg tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt
    15061 gaaggaatca tgcagttggt tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca
    15121 gctgaaagcc ttcgtagcga atttgttatt gaggtgactg gtcaggtcgt tgcgcgtgag
    15181 caagccaatg ataagttgcc aactggtgcg gttgagttaa acgtgacagc tcttattgtg
    15241 cttaacacag ctaagacaac accatttgag attaaggatg gcattgaggc aaatgacgat
    15301 acacgtttgc gttaccgtta ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa
    15361 cttcgtgcca aggtgaccca ctctatccgc aactacttgg atgagttgga gtttatcgac
    15421 gtggagacac cattcctttc taagtcaacg cctgaagggg cgcgtgatta tttagtgccg
    15481 tctcgtgtta ataaggggca tttttacgct cttcctcaaa gtccacaaat cacgaaacag
    15541 ctcttgatga atgctggttt tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag
    15601 gacttgcgtg gagaccgcca gcctgaattt actcaggtcg acttggaaac gtccttcctt
    15661 actgagcaag aaatccaaga tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca
    15721 aaaggcatcg aagtaacgct accgttccct cgtatgaaat acgatgatgc tatggctctt
    15781 tacggttctg acaagccaga tactcgtttt gacatgttgc ttcaggactt gacagaagtg
    15841 gtcaaaggtg tagactttaa agtcttttca gaagcacttg ctgtaaaagc gattgtggtc
    15901 aaaggagctg cggacaacta ttcacgtaaa gacatcgaca agatgacgga agtagccaaa
    15961 cagtatggtg ccaaaggtct tgcttgggtc aaggtggttg atggagaatt aaacggacca
    16021 gttgccaagt tcttgactgg tatccaagaa gaattgacaa cagcgcttgc tcttgaagat
    16081 aaggacttgg ttctctttgt ggcggatacg cttgaagtgg ctaatgcaac actgggtgcc
    16141 cttcgtggac gtattgctaa agagcttggc ttgattgata atgataagtt caacttcctt
    16201 tgggtggttg actggccgat gtttgaatgg tctgaagaag aaggtcgcta catgagcgcc
    16261 caccatccat tcacacttcc acaggaagag actgctcacg aattagaagg tgatttggct
    16321 aaggttcgtg ccattgctta cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc
    16381 cttcgtatca accaaaaaga ccttcaagaa cgcatgttca aggctcttgg tttctcagct
    16441 gaagaagcca atgaccagtt tggtttcctt cttgaagcca tggactatgg tttcccacca
    16501 cacggtggtt tggctatcgg gcttgatcgt tttgtcatgt tgcttgctgg agaagaaaat
    16561 atccgtgaag tcattgcctt tcctaagaac aacaaggcaa ctgacccaat gacacaagct
    16621 ccatcaacag tcgctctcaa acaactagag gaactcagct tacaagtaga agaagatgaa
    16681 acaagcaaaa cgaattaagc ggtggcgcta ttatctgcgc cgctttgctt atcagataaa
    16741 aattttacgt gtcttacaaa gtatctctcg agaaaagtat gatgagaaga tttcggcctc
    16801 tctggtctat ggttttttat cagcagtagc agttaatttc tttttccaac cagggcatgt
    16861 gtattcgagt ggtgcaacag gtctggcaca gattatctct gccttgagta atcactggtt
    16921 tggttttcat attccgattt cgctaagctt ctacgccatt aacttccctt tgatggtctt
    16981 agcttggtat cagattggcc ataagttcac cgtctttacc tttatcacgg tatctatgag
    17041 ttccttcttt atccagtttg tccctgtggc aaccttgacg gaggatccca ttatcaattc
    17101 cctttttggg ggtgttgtta tgggtttggg gattggtttt gctcttcgaa acaatatctc
    17161 cagtggtggg acggatatcg tcagcctgac tattcgcaag aaaacgggta agaatgtcgg
    17221 tagtatttct ttcttggtaa atggaactat catgctgata gcaggtttaa cctttggttg
    17281 gaaatacgct ctttattcta tgattaccat ctttgtctct agccgtgtga cagacgcagt
    17341 ctttactaag caaaagcgta tgcaggccat gattgtgaca aatcatccag agaaggtaat
    17401 tgaaaaaatc cataaaaaat tgcaccgcgg agcaaccatg atccacgatg cagaaggaac
    17461 ctataatcac gagagaaagg cagttttaat cactgtcatt acacgtgcag agtttaatga
    17521 atttaaacag attatgacac aagtggatcc aagctccttt gtctctgtct cggaaaatgt
    17581 tcatattcta ggaagatttg ttgagataga taattagtga ttaatactct tcgaaaatct
    17641 cttcaaacca cgtcagcttc accttgccgt atatatggtt actgacttcg tcagtttcat
    17701 ctacaacctc aaaaccatgt tttgagctga cttcgtcagt tctatccaca acctcaaaat
    17761 agtgttttga gcaacctgcg gctagcttcc tagtttgcac tttgattttc attgagtata
    17821 aaaagatctt gggaaaagac tcaatttcag tagaaaagga agtaaatctt cccacaataa
    17881 aacgcatagt atcaagtttt ttcaatacct gatagtatgc gtttttgctt ttaacgactt
    17941 tttgcccagt ctcttatttt tcgataattt tgtgggcgat caattgacgg taacaaattt
    18001 gtttattgtt tttattatca ttaataatct ggagaatagt ttcaagggaa acacgaccaa
    18061 gctctaaact attgatatcg acataggctg ccaagttgag cttgggatta accgagtcaa
    18121 agctgagaac agggacatcc agctggtgtt tggcaatata gttacaaaca ccttcagcta
    18181 ggaggctatc ggttgtgatg atagcatcaa tttgtggatc gtgcttgaat aatcgcttgc
    18241 taaatttata gcccttttct tccagaaact cgtcggcaaa gtagatgcga ttgttgtcag
    18301 tggtaagttt gtaatgttta agcgcctgtt catagcctgt taaacggtct ttggtcacga
    18361 agagcttttt acttcctccg ataaaggcaa tgcgtttgca gccttttttg atgaaatatt
    18421 cagtcgcatc aaaaccagct tgaacattgt cgttgtcgac aagtgggatg aaaggagata
    18481 gagatttacc taggataagg aaggggaact gttcttctgc gacgagtttt acgagagggt
    18541 cttcttcttg ggcatagaga aagattagcc catctacacg cttgccgtag accatttgtg
    18601 aaatagcgtt gagacgctcc ttctcatctt tccctgttgc tatctgaatg gcatagtggt
    18661 tttcagatgc gacttgagag atgccacgta gaaccgatgg aaagaaggga ttctggtaga
    18721 aggcgtctga gtcatcagga agaactaatc cgataacctg agtatagctg cttaccaagc
    18781 tacgagcgtt gaggtttggg tggtagttga gttccttcat agctttgcga acgcgttttt
    18841 ttgtttcgtc gctaatggtt gatttatttt gaataacacg ggttacggtt gaaggcgaaa
    18901 caccagcagc cttggccacg tctttaatcg taacgggcat aaaaatctcc tatttatggt
    18961 aatttggatc acggaaatgt gttttataaa agatagtgac cagatacaga acaaaaagaa
    19021 tattttgtac agtaatcagg gttgtcatat tttggccaaa taatcccaaa ataagtgtaa
    19081 tcagagtcgg caatcctaaa cagttcaaga taaagtgata gcactcttta aaggttctaa
    19141 atgaaaagag gcgtgatttc ttagtgatat aaaggagaag actagctcct agagagacga
    19201 taaagaaatt caaaccaaag aggaagctcg caccgagaac taggaagagg ctgatataga
    19261 cacgattttg ttggtaccag tctttagaaa ttgcttgggt caagctgtct ttgcttttga
    19321 aactctcagt ctgaatggct cggtaagaga tgcgggtcag ctccttgctt tccttgctga
    19381 tgactagctc atttgtatcg aaatgcagtt gcaagtcctt aggtaattcc ttgatttgac
    19441 ttggaccaat cacaacagaa ggggtttgac tagctgttcc agtataagtt aatgtgccat
    19501 cgacaattgt agcatgttca gagagatcct ggacaacctc atttgtcaga ggttcataga
    19561 cattatcgat aaaagtttct agcggatagg tctcctggga gctgttttgg atagcaatgg
    19621 gtaccataga caagctgata aggaagatac tggtaaagag aagttgaaac cagttgagct
    19681 cgaaacgttt ggacaggggc ttacgaaacc cccaaatact tgaaaaatag gaaaatggat
    19741 atggaagcat aggtatcttt ctaaaaggtg ttttctatat gagtattatt atatagagaa
    19801 atgcgcttta tttcaagtct aggagaaaaa ttgcttgcta caaggatatt atttacagtg
    19861 acaatcgcta atagtcaatg aaaatcaaag tgcaaactag ccacaggttg ctcaaagcac
    19921 aactttgagg ttggagataa agctgacgtg gtttgaagag attttcgaag agtataaaat
    19981 gaaaaagggt ggcggggata aattatccct tgtcgccacc acttgtaagt cctgaaacaa
    20041 agttcttttg taggaagaag aagagaatac agattggaag ggcgatgagg atagcacctg
    20101 ctgagaagta ggcaatcttc aagtttttcg cattgttaac gaaggtttgg agacctacgg
    20161 caacagtaaa gtattctttc tcacgaagca agaaactaga gaggatgtag tccccgaaag
    20221 gtcccatgaa ggcccagaga gcttgtacgg caaccattgg gcgaacaagt ggaagaacaa
    20281 tttgccagaa gcggcggaag tgtcctgcac cgtctagttt tgcagattcg tctaaagaca
    20341 ttggcactgt atcgaagtag cctttcatga gccaagcatt catcgggata ccaccaccaa
    20401 cgtagaggaa gatgaggaac cagttgtggt taagggcgtt caacataagc gccataacga
    20461 agaaggctgt caaagcggcc atagttggca ccatttggat gatcaagaag aagaccaaac
    20521 tttgtttacg agccaagaag ttgtaacggc tgtaagcata accagcaagt acgatgatac
    20581 ttgtttgaac agccatggta attaaggcga taatcaaagt gttgaggtac caagtaccgt
    20641 acaaggtttc agtgaagagg cctttaaagt tatcaaaatt gaggtcgatg ttagtatcta
    20701 gtttaaaggc tgagacgtta cctgctttaa aggctgacat aatggtaatc aacagtggat
    20761 agataattac aattgataga ccaatcaggt aaaggtaagt aaggctttga gtcagtctac
    20821 gtttgagttt aattgagtta ttcatcttag acgtcctcca tatcaaatgc gtgtagtttc
    20881 ttgaatgcga tcatagagat tgaaatgaca atgatagaga tgatcaaggt aacagctgcc
    20941 gccattgagt attgaggaga tgtacctgtt gtcaaacggt agatccatga gatcaagata
    21001 tcggttgaac cagctccacc tccgacacta ccaggtcctc caccattgaa gaggtacatg
    21061 atagagaagt tgttaaagtt gaaggtgtat tggctaatca aagtaggtgc cgcaacagcc
    21121 aaaatcattg ggaaagtgat gttgcggaat ttttgccaag cgttggcacc gtcaatataa
    21181 gctgcttcgt aaaggtcgtt aggaatagat tgcaagatac ccaaggtcag aacgtagatg
    21241 tatgggaatc cgagccaacc ttgcatcata atcaaggcaa tcttagtcca agttgggtct
    21301 gttttccaag gaataagagc tccatcaagg aaaggaagga atttagccaa gattggcaat
    21361 acttgagtgt tgatagcacc gacactatcg ttaaacatgt ttgagaatgt caagatagtg
    21421 atgaaggctg ggacagccca aggaagaagg aaaataacac caaagatacg ttttcctttg
    21481 ataaatggtt ggttagcaat gatagctgtg aagataccaa ttacgatttg taaagtagaa
    21541 gctgccaaag cccaaatgat agtccaagaa agaacagaac caaaggcaga acggaaggta
    21601 ctcaagctcc aaatgtttgt aaagttggtc aaaccaaccc agtccaacaa cttgtttggt
    21661 ggcaagtgtt ggaagtcgta gttggtaaag gcgatcatca aggttacgat aactgggaag
    21721 ataatcgcga aggtcatggc aacataagat ggaatgatca agaggtaagg gaagccattt
    21781 tcataaatcc ctttgatcat gtctttgagt gtgcgtggaa ctggaattcc attgttaatg
    21841 cgttttgcaa tcgtatgtgc atctttgata tttgagaaat aaaagagtac ataaacgatt
    21901 acaaagatta gatggaaggc accacgaatc agcataaaga gggaattatc acgacctggt
    21961 ttgtcaccaa gagtgatgag gttgctcaat tcaggggttg caagtgctag gaagtaaagg
    22021 acaaatacga tggttacacc aaggaagata aaacctttgg ctttttgttt attgtaaatc
    22081 tgtcctaacc caggaatgat agacagcagg gctgctttac taggttgttg cttttccata
    22141 ttaactcctt tcataggata ctactttttt ataagaaagc taaattattt gtgtctctat
    22201 tgataaattc aaagggggat ttgatttcca ccccccttga acaaattttt tattcaccaa
    22261 atttttgttt gattgtttct ttgatcaatg ttacagcatc gttagcagct gttttagcat
    22321 ctttttgacc acttacagca tcaaagagca tatttttcgc tggatcccaa actgcagaca
    22381 tttgagagat gtttggcagt ggttgagtgt tcttgaactg tttgataaca gctgttgtca
    22441 actcatcgtt tttaccttca gcgtatgaac gagcctcagt attagctggg atttcgttag
    22501 tcttatcata taatactttt tgttgttcag ttgcaacaag gaagtctaca aatttttgag
    22561 aagcttcaag gttcttaacg gcttgaggaa tgacccaagc tttaccacca ccgaatgcag
    22621 catattcttt tccatttgga agagttggga tagttgcaac tccgtagttt actttagcat
    22681 ctttaaaggc ttgagctttc caaggtccgt cgatgatagc agctgtttta ccttcttgga
    22741 attgagtttg gattaagttt ccagcacctt ctgtatcttg cataccttta ggccattttt
    22801 cgtaccaaga tttagcgtag ttgatacctg cgatagaacc gtcgtttgca agaccgatgt
    22861 ctttagcgtc tttaccgttt tggccaaaga cgtaagcacc gttaccggca agaagtccat
    22921 atgtatagta gaagtttgtc cagtcagcta ggaaggcagt agttttacca tcttcaccag
    22981 cgaatgcgta tttgctatct ttagcaaggt tttccaagtc agcaaatgtt tttggagcat
    23041 ctttcaccaa gtctttgttg tagtacataa caagtgactc gataacggca ggagcaccgt
    23101 aaactttacc attagcagct gttacaagag atttagttgt gtcgtctgtt ttagcaccat
    23161 cgctcaattt cacttctgaa agttgtccgt cagaaccaag gctacctaca cggtcgtatg
    23221 gagccatcat aacatcaggg acattaccag attggttgtc aagagaaagt ttatcaagac
    23281 ctcctagagc atcaccagtt ttaagagtga cttttactcc agcttctttt tcataagctt
    23341 tagcaacctc ttcaatatag ctcttatatc cctcgtctac atatacagtg agttctttga
    23401 cttcagatga accagaatca gcaggcttat cagcagtttt gcttccgcaa gctaccaaaa
    23461 gcaagctagc aagtgtagca gttccaagca ccgcagtgct cttcataaat ttagatgaca
    23521 tagtgtattc ctcccaaaga atagcaagtt ttattgataa ggaaacgcaa acgttttcct
    23581 ttatgagctt agtatagcac aaatagaaaa cggttgcaag tattttttgt aaaaatttaa
    23641 aaaaattttt aagcttgatt tgatagcata attttacatt ttaatagaag aaaagtatga
    23701 aatagataag aatacagtat ttttaataaa aatatatgga atctgtctaa agagggaaat
    23761 caggaggggc tacctccctg tttctaatcc ctatctattt tctatagacg tttgtgcttt
    23821 gttgcatata aattcaagct ccctaaggag gttttgtact ttgtagactg tctcgtgaga
    23881 gtcaaacttt aggattagat agttttaagt tatctttgct aaaatgagtt agtctcctta
    23941 aaaataatca aaaaagtttt ttaaaaacgc ttgcaattat gcttgaaaag gagtatactt
    24001 ataagtaacg caaacgtttg cgtctgcaaa atacgcaacg ttccattatt ttaacacacg
    24061 aggtgctatt atgaaaaaac gtcaaagtgg tgtgttgatg cacatctctt ctcttccagg
    24121 agcttacgga atcggatcat ttggtcaaag tgcttacgac ttcgttgatt tcttggtccg
    24181 tacaaaacaa cgttactggc aaatccttcc attaggagca actagttacg gggattctcc
    24241 ttaccaatct ttctcagcct tcgcaggaaa cactcatttt atcgatttag atatcttggt
    24301 ggagcaaggt ttgttggaag caagtgacct tgaaggagtt gactttggta gcgatgcgtc
    24361 tgaagttgac tatgctaaaa tctactatgc acgtcgtcct cttttagaaa aagcggtgaa
    24421 gcgtttcttt gaagtcggag atgttaaaga ttttgagaaa tttgctcaag acaaccaatc
    24481 atggcttgag ctctttgctg agtatatggc tatcaaagag cattttgaca atcttgcttg
    24541 gactgaatgg ccagatgcag atgctcgtgc tcgtaaagct tcagcacttg aaagctatcg
    24601 tgagcaattg gcagacaagt tggtttacca ccgtgtgact caatacttct tcttccaaca
    24661 atggttgaaa ttgaaagctt acgctaacga caaccacatc gaaatcgttg gggacatgcc
    24721 aatctacgta gcggaagatt caagtgatat gtgggcaaat ccacatctct tcaaaacaga
    24781 tgtcaatggt aaggctactt gtatcgcagg atgtccacca gatgagtttt ctgtaactgg
    24841 tcagctttgg ggtaacccaa tctatgactg ggaagcaatg gacaaagacg gctacaaatg
    24901 gtggattgaa cgcttgcgtg aaagcttcaa aatctacgat atcgttcgta tcgaccactt
    24961 ccgtggcttc gaatcttatt gggaaatccc tgctggttcc gatacagcag cacctggtga
    25021 gtgggtgaaa ggtccaggct acaagctttt tgcagccgtt aaggaagaac ttggtgagct
    25081 aaacatcatc gcagaagacc ttggcttcat gacagatgaa gtgatcgaat tgcgtgaacg
    25141 tactggcttc ccaggaatga agattcttca atttgccttc aacccagaag acgaaagcat
    25201 tgatagccca cacttggcac ctgctaactc agttatgtac acaggaacac acgataacaa
    25261 tacggttctt ggttggtacc gtaatgagat tgatgatgcg actcgtgagt acatggctcg
    25321 ttacacgaac cgtaaagaat acgaaacagt ggtacacgct atgcttcgta cagtattttc
    25381 atcagttagc tttatggcaa ttgcaactat gcaagattta ctagaattgg atgaggcagc
    25441 tcgtatgaac ttcccatcta cccttggtgg aaactggtct tggcgtatga ctgaagatca
    25501 attgacacca gctgtcgagg aaggtttgct tgacttgaca acaatttatc gccgaattaa
    25561 tgaaaatttg gtagatttaa agaaataaga caataatcag gagacaacta aacatgttat
    25621 cactacaaga atttgtacaa aatcgttaca ataaaaccat tgcagaatgt agcaatgaag
    25681 agctttacct tgctcttctt aactacagca agcttgcaag cagccaaaaa ccagtcaaca
    25741 ctggtaagaa aaaagtttac tacatctcag ctgagttctt gattggtaaa ctcttgtcaa
    25801 acaacttgat taaccttggt ctttacgacg atgttaaaaa agaacttgca gctgcaggta
    25861 aagacttgat cgaagttgaa gaagttgaat tggaaccatc tcttggtaat ggtggtttgg
    25921 gacgtttggc tgcctgcttt atcgactcaa ttgctactct tggtttgaat ggtgacggtg
    25981 ttggtcttaa ctaccacttt ggtcttttcc aacaagttct taaaaacaac caacaagaaa
    26041 caattccaaa tgcatggttg acagagcaaa actggttggt tcgctcaagc cgtagctacc
    26101 aagtaccatt tgcagacttt actttgacat caactcttta cgatattgat gttactggtt
    26161 atgaaacagc gactaaaaac cgcttgcgtt tgtttgactt ggattcagtt gattcttcta
    26221 ttattaaaga tggtatcaac tttgacaaga cagatatcgc tcgcaactta actctcttcc
    26281 tttacccaga tgatagtgac cgtcaaggtg aattgctccg tatcttccaa caatacttca
    26341 tggtttcaaa cggtgcgcaa ttgatcatcg acgaagcaat cgaaaaagga agcaacttgc
    26401 atgaccttgc tgactacgca gttgtccaaa tcaacgatac tcacccatca atggtgattc
    26461 ctgaattgat tcgtcttttg actgcacgtg gtatcgagct tgacgaagca atctcaattg
    26521 ttcgtagcat gactgcctac actaaccaca caatccttgc tgaggcgctt gaaaaatggc
    26581 ctcttgaatt cttgcaagaa gtggttcctc acttggtacc aatcatcgaa gaattggacc
    26641 gtcgtgtgaa ggcagagtac aaagatccag ctgttcaaat catcgatgag agcggacgtg
    26701 ttcacatggc tcacatggat atccactacg gatacagtgt taacggggtt gcagcactcc
    26761 atactgaaat cttgaaaaat tctgagttga aagccttcta cgacctttac ccagaaaagt
    26821 tcaacaacaa aacaaacggt atcactttcc gtcgttggct tatgcatgct aacccaagat
    26881 tgtctcacta cttggatgag attcttggag atggttggca ccatgaagca gatgagcttg
    26941 aaaaactttt gtcttatgaa gacaaagcag ctgtcaaaga aaaattggaa agcatcaagg
    27001 ctcacaacaa acgtaaattg gctcgtcact tgaaagaaca ccaaggtgtg gaaatcaatc
    27061 caaattctat ctttgatatc caaatcaaac gtcttcacga gtacaaacgc caacaaatga
    27121 acgctttgta cgtgatccac aaataccttg acatcaaagc tggtaacatc cctgctcgtc
    27181 caatcacaat cttctttggt ggtaaagcag ctccagccta cacaatcgct caagatatca
    27241 ttcacttgat cctttgcatg tcagaagtta ttgctaacga tccagcagta gctccacact
    27301 tgcgagtagt tatggttgaa aactacaacg ttactgcagc aagcttcctt atcccagcat
    27361 gtgatatctc agaacaaatc tcacttgctt ctaaagaagc ttcaggtact ggtaacatga
    27421 aattcatgtt gaacggagct ttgacacttg gtactatgga cggtgctaac gtggaaatcg
    27481 ctgagttggt tggagaagaa aacatctaca tcttcggtga agattcagaa actgttatcg
    27541 acctttacgc aaaagcagct tacaaatcaa gcgaattcta cgctcgtgaa gctatcaaac
    27601 cattggttga cttcatcgtt agtgatgcag ttcttgcagc tggaaacaaa gagcgcttgg
    27661 aacgtcttta caatgaattg atcaacaaag actggttcat gactcttctt gacttggaag
    27721 actacatcaa agtcaaagag caaatgcttg ctgactacga agaccgtgac gcatggttgg
    27781 ataaagtcat cgttaacatt tctaaagcag gattcttctc atctgaccgt acaatcgctc
    27841 agtataacga agacatctgg cacttgaact aatactcttc gaaaatctct tcaaaccacg
    27901 tcagctttat ctgcaacctc aaagcggtgc tttgagcaac ctgcggctag cttcctagtt
    27961 tactctttgg ttttcattga gtataagata caaatttata ctaatacatt ttgtaaaaaa
    28021 gcgagtttcg attgaaattc gcttttttaa atgctgtaga tttgggtcaa tcttgtctaa
    28081 aaatagggaa atcctagata cagtgaagac tttaaatgct ggtttttact gtcctcagcc
    28141 ttatattttt tcgtagttga ttacctcaca cctcttgtat tcgcttacat aaagtattat
    28201 aatataattg taggaaagaa ggtgttttta tgatatacac acttaaattg gtgttgttta
    28261 ttacctttct tgtaataagc ttgttacctg ataagatttt tggaaaaaat aaaaaaattt
    28321 ggaaaatagt ttttgcaata ttgacggcag tggcagcatt gtcatttatg tactaagtta
    28381 ttttaagaat gtagggaaat aaaccctaca ttctttttag ttttttctgt tttctaaatt
    28441 ctatttatcc aagcgcttca acatttcttg cttcttcgct tcaagttctg cacgcttttc
    28501 ttcgatttcg gcatgttttt tctcgagttc agaacaactt gcaccattgc taaattcttt
    28561 tcgccatcag gagatagggt gagtcgacat gtctattact cacccaaagc agtcctacaa
    28621 agcaggaatt ttctgttact tttttggaaa tagtaacgtt tatacagctt tgacacttcg
    28681 tatcaaagcg ccaaacacac tccgagggat ttacagaaag cagaaaagga atgatctggt
    28741 ataagatcat tccttttctc tctttttctt taagtaatta tatacaatgt acgacgaagt
    28801 cgtcattgca atgctgatcc accacctaaa gggaacttta aacaacattg ataagataaa
    28861 gaatataaac aacgaaaata cgttataccc aattaatttt attgtatatc tcatgattaa
    28921 aagttaatcc ttccgttgtt aggaatggca tcatttttat cccataattg tgctaaataa
    28981 gtccccggtg ataataaatt catagcggat tctaaagcaa catcatttac aaaccaacta
    29041 cctagatatc tagaaattgc tgaacgaata gcactttttg ctgcatgttt tccttttact
    29101 ttaattagat ttgcaaggcc tgcagtagtt cctcctaatg ctaaagctat tgcagtatct
    29161 aatagagcac ccatttgatt aactgtaata ccttgccaag ctgctctaaa tggagagtat
    29221 gtaggtggga ttgtataatc gccttgtaat tgtcggttaa ttacttcttt gatccattgt
    29281 tgtgagacgt ctggatgaaa agattggatt tcgtttgcaa gtgtattgat ttgttcttct
    29341 gttagagaag tgacaggttg aagttccata tttgtttcaa tttgtgatac ttgttcagaa
    29401 gcgtatacag ctgaaacact tggaatcgct gatacaatta acacaattga cgtcaaaaaa
    29461 accgaaataa atttcattaa tttgttcatg agcttttctc ctttttattt gtatctgctt
    29521 acattttatt atatactgtt attatagtca agaaaaatat gctattatgt taaaaaaata
    29581 tttttaaaaa tataaatgga cggatttatt ttggatttta tttgttattt tgacctgcct
    29641 ctatattggt aaccatgatt tgtttactct caatcatcaa gaattctctt ttcgtggtag
    29701 cgtttggggt ctggtactgg ccttatatca cttattgttc attgataagt ttgtaatttc
    29761 gaatcgaaaa taaagattag aactatgatt gactggctac ttttaatatt gattttggag
    29821 gaagattttg tctctattat ttattatttt aaatttattt attttgtata agatctattc
    29881 tttgaggcgg gaaaaatcga aatacttgat ttatacggcc tatatcatat ttggggtaaa
    29941 tgtagtatat ggtattcaat ggttattaag agaactgatt tcaactattt ccccttaatg
    30001 tgtatggaaa ctataacaaa acgcataaga ttaaggtttg tgtacctgtt cttatacgtt
    30061 tttgtaatat tgaagatttt cggtaagttt tatacctttg ggaagtctag tttagaatgc
    30121 tttcccaacc aaaatctctg cttcgatggt aatctctgtc agttggctcc agtcaatctt
    30181 gctctggtca acatgaaaga ggagaggagt catactgagt agggcttgga gttgttctgc
    30241 tgtgatagtc ttagtcagag aggcagtttg actagatagg atggtaaagt gctcctggaa
    30301 atgctccttg atatcttggt tagaatactc cttgtttgtc agctggtcct gtactctttg
    30361 acggatttct ttgaggtgat tttcagttgg gataaccttt atcaagatac cgtctttgga
    30421 taaaacgcga cgaaattctc catagttggc aggtgagaag atatcaagca gaatatccat
    30481 gttagcgtct tttataggaa gtcgtgccaa gtcgccaacg aaccaattga ctgcccagtt
    30541 gggttcactt ttagcagcga tttggactga atctttggag atgtcaaagg catagaaagt
    30601 tttttcagag tgactttctt gtagtttgcg agaatagaat ccttcaccac aaccgatatc
    30661 caaaattgtt gtggtagttt ttgagcttgc aagcaagtca gatacagcat ctaagatagc
    30721 ttggtaaaag ccggcttcta ggatttgttg acggttttga aaattttcct tgtcgtagtt
    30781 agcagattgc ttgatttgag gtgctagatt gacatagcca aatttcgcca agtcaaaaga
    30841 atgacggttg cagcacttga aattagtctc taacagagtc agattttctt gacagatagg
    30901 acaggcaaag gcagtcgcag aagcaaaacg ctgaagtttg ggcttgagat ttgtattcat
    30961 agtttaagtg tatcaaaaga ggcaaatgaa agcaacttta ggaataagta gatgggagat
    31021 tcagtagaaa taaaactaat tctccaattt atagaatgaa attgctttta tatctaaatt
    31081 gctatataat aatgataagg aggaaataag tatgttaaaa gaagtattaa ccgttgcaaa
    31141 agttgcgaaa aaatcttcat tatttttggg tggtgtcgca tttggtaccc ttggtttgaa
    31201 aatcttagca agtaaggaag ctaaaaaagg ttattctaaa gctttggcta aggcttacaa
    31261 gttgaaagac gagctagatg catctgtttc tgttgtgaag caacatggag acgatgtctt
    31321 gcaagatgcc aaatatttgt acgagcaaga gaaaaaagaa gagcaattag atagccttat
    31381 aggtgaataa tatgtctttt aaagtgctac atagaggata ccaacatatc cgactatcat
    31441 cttctttttc acttaccttg gatattcaag actatcttcg ttccttggcg agagatgaaa
    31501 aggggattga gtctatccag ttttacatgg atcaacagca ctttactcta cgcataaaag
    31561 aaggcttttc tgtattagat aatgcagaag cctttttaaa aagaattgat aaagggaaag
    31621 tttctgagtt gatgactctt cccattcgta gagaagagag tgcttattct attgtttcag
    31681 gtgcagcggt taagcgtgta ctttttcgta gttttgtgcc gtatcctatt cgctatatat
    31741 ggacttgtta tcaggctttg ggttatatta gagaagccta tcaaacacta gcgcgtaagg
    31801 aactaacgat ggaagtcttg gactgttcgg ctattttatt gtccttgttt atgaaccaat
    31861 ccaagacagc tagcaatatc atgtttatgc ttgatttggg gaatcattta gatcagtggt
    31921 ccttgaaaaa aactgcaaca gatttagaac agagtcttct tgcaaaagag agcgatgtat
    31981 tcctagtaca gggcgatacg gttgttagta taaagagttc cgatgttcaa ataggagatg
    32041 tcttgatctt atctcaagga aatgaaattc tgtttgatgg gcaagtagtt tcaggtttag
    32101 gtatggtcaa cgaaagttcc ttgacaggag agagttttcc agttgaaaaa agagagtctg
    32161 atttggtttg tgcaaataca gtattagaaa ctggagagtt acgcattcgt gtaacagata
    32221 atcagatgaa cagccgtatt ttacagctga ttgagttgat gaagaaatct gaagaaaaca
    32281 agaaaacgaa acaacgctat ttcatcaaga tggcggataa agtcgtcaaa tataatttct
    32341 tgggggctgg gctgacttac ctattgacag gttctttttc taaggctatt tctttcctat
    32401 tggtcgattt ctcctgcgct ttgaaaatct ctactcctgt agcttatttg acagttatca
    32461 aggaagggtt gaaccgtgaa atggtgatta aggatggaga tgttctggag aaatatctgg
    32521 aagttgatac tttcttgttt gataagacag gaacaatcac aactagttat cctatagttg
    32581 aaaaggtgtt accttttgga gactatagtg aggaagatat tctcagaatc agtgcctgtc
    32641 ttgaggaaca catttatcat cctattgcta atgccatcgt caagcaagct gagatagagg
    32701 gaattgaaca tgaggaaatg catgggaaac tccaatatat cgcaagcaag gggatcaaat
    32761 ctcatataga tggccaacca gttcttattg ggaattatgt cttgatgcag gatgagcaga
    32821 ttcatatcag ttcagaacaa aatgctttaa ttgaagagta caagagtcac tacaatctct
    32881 tattcttggc ttatcaaaat gaattgattg gaatgttctg cattcatact cctttgagaa
    32941 aagaagcaaa aacagccttg gataaactta aggcacaggg gaaaaaattg attctggcaa
    33001 caggagacac cttgattagg acagaggaat tagtcaaaga tttgcccttt gatcaggtct
    33061 atacagactt gaaacctgat gggaaatttg agttagtaga gaaactgcag aaagcaggtc
    33121 acactatttt gatggttgga gatggattga atgactcagc ggctctaact ctatcagata
    33181 tcggtgtggt gatgaatgag agtgcagata tttctaagca gatgagtgat atcttattgt
    33241 tagataatcg tttggatttc ttccaagagt tggattcgtt atcatcatct ttgcaaacac
    33301 tcatcaagaa aaatattcaa gataccgttg tcgtaaatag tagtttgatt ggctttggct
    33361 tatttaattg gctcagtcct tcaaatctct ctatcttaca taatctaaca accttgcgca
    33421 ttgtcctgcg tagcctgtct attaaaaata gatagatgag agttattaac agcaaaaagg
    33481 agttaccttt ctcgaggtta actcctttgt ttatagataa atgttgaata gtttagtaag
    33541 tcaatacaaa gtattttttc ttcccacgac ggataacagt cagttcattt tctaacttat
    33601 cagcgtcact caagacatag tcaaggtctt ggatgcggcc gccgtttacg tagatggctc
    33661 cgttttggac gtcttcacgg gcttggcgtt ttgagttaac tataccagat gagacgagca
    33721 gttccacgat attgttgtgt tcgtctgcct gtacttggta gtttggcaca ccacgaagtc
    33781 cttgtttgag ctctttgaca gaaaggtttt tgatgtttcc tgcaaagagt tgctcagtga
    33841 tgttaagtgc ttctttgtag gcttcttctc cgtgaacaag tgtaacaact tcacgagcca
    33901 agactttttg agccaagcgt tcgtgtggcg ctgcttcaaa ttgtttgcga atatcttcaa
    33961 tctcatcaag tgacaagaaa gtaaagattt tcaagaagcg aacagcgtca gcgtccatca
    34021 cgttcatcca gaattggtac atttcgtatg gagaagtctt ttcgggattg agccagacgg
    34081 catttccttc tgatttacca aatttcttac cagttgcatc tgtgattagt ggaacagtga
    34141 taacgtgacc agtcttgtcc gccttacgac gaagcaattc ggtaccagct gtcatatttc
    34201 cccactggtc agaaccaccg atttgaagag tgacattatg gtcttggtta aggacgaaga
    34261 agtcgtaccc ttgcatgatt tggtaagcga actcagtgta agaaattcct gtttcgatcc
    34321 gttttttaac agattcctta ctcatcatgt agttgaccgt gaagtatttt ccaatatcac
    34381 ggaggaagtc aatgaagctg atgctgccaa accagtcgta gttgttgacc atgacagcct
    34441 tgttttcgcc attttcaaag tcaagaaaac gagaaagttg tccttggata gacttgaccc
    34501 agccatctac tgtgtctttt gtttggagac tacgttcagc atctttgaag gacggatctc
    34561 cgatgagacc tgtagcaccg ccaacgagcg cataaggttt gtgacctgct agttgcaagc
    34621 gacgacttgt caagattgcg acaaggtggc ctaggtgaag gctgtcagca gttggatcgt
    34681 agccagtata ataagaaact tgaccttctt ctagggcttt acgcaaagct tcttcatcag
    34741 tcgtttgaaa tatcaaacca cgctctttta gctcatcaaa aatgtgcatg tgtcttttct
    34801 cctttttaaa attattgttt ctacctattg tatcacaaac ttgggcaata gcctagcaga
    34861 atagggaaga aagtggctat tttattgaaa atttcccttt tgtggtataa tagataaagt
    34921 gaggaaatcc atgcaaaatc aattaaatga attaaaacga aaaatgctgg aatttttcca
    34981 gcaaaaacaa aaaaataaaa aatcagctag acctggcaag aaaggttcaa gtaccaaaaa
    35041 atctaaaacc ttagataagt cagccatttt cccagctatt ttactgagta taaaagcctt
    35101 atttaactta ctctttgtac tcggttttct aggaggaatg ttgggagttg ggattgcttt
    35161 ggggtacgga gtggccttat ttgacaaggt tcgggtgcct cagacagaag aattggtgaa
    35221 tcaggtcaag gacatctctt ctatttcaga gattacctat tcggacggga cggtgattgc
    35281 ttccatagag agtgatttgt tgcgcacttc tatctcatct gagcaaattt cggaaaatct
    35341 gaagaaggct atcattgcga cagaagatga acactttaaa gaacataagg gtgtagtacc
    35401 caaggcggtg attcgtgcga ccttggggaa atttgtaggt ttgggttcct ctagtggggg
    35461 ttcaaccttg acccagcaac taattaaaca gcaggtggtt ggggatgcgc cgaccttggc
    35521 tcgtaaggcg gcagagattg tggatgctct tgccttggaa cgcgccatga ataaagatga
    35581 gattttaacg acctatctca atgtggctcc ctttggccga aataataagg gacagaatat
    35641 tgcaggggct cggcaagcag ctgagggaat tttcggtgta gatgccagtc agttgactgt
    35701 tcctcaagca gcatttttag caggacttcc acagagtccc attacttact ctccttatga
    35761 aaatactggg gagttgaaga gtgatgaaga cctagaaatt ggcttaagac gggctaaggc
    35821 agttctttac agtatgtatc gtacaggtgc attaagcaaa gacgagtatt ctcagtacaa
    35881 ggattatgac cttaaacagg actttttacc atcgggcacg gttacaggaa tttcacgaga
    35941 ctatttatat tttacaactt tggcagaagc tcaagaacgt atgtatgact atctagctca
    36001 gagagacaat gtctccgcta aggagttgaa aaatgaggca actcagaagt tttatcgaga
    36061 tttggcagcc aaggaaattg aaaatggtgg ttataagatt actactacca tagatcagaa
    36121 aattcattct gccatgcaaa gtgcggttgc tgattatggc tatcttttag acgatggaac
    36181 aggtcgtgta gaagtaggga atgtcttgat ggacaaccaa acaggtgcta ttctaggctt
    36241 tgtaggtggt cgtaattatc aagaaaatca aaataatcat gcctttgata ccaaacgttc
    36301 gccagcttct actaccaagc ccttgctggc ctacggtatt gctattgacc agggcttgat
    36361 gggaagtgaa acgattctat ctaactatcc aacaaacttt gctaatggca atccgattat
    36421 gtatgctaat agcaagggaa caggaatgat gaccttggga gaagctctga actattcatg
    36481 gaatatccct gcttactgga cctatcgtat gctccgtgaa aagggtgttg atgtcaaggg
    36541 ttatatggaa aagatgggtt acgagattcc tgagtacggt attgagagct tgccaatggg
    36601 tggtggtatt gaagtcacag ttgcccagca taccaatggc tatcagacct tagctaataa
    36661 tggagtttat catcagaagc atgtgatttc aaagattgaa gcagcagatg gtagagtggt
    36721 gtatgagtat caggataaac cggttcaagt ctattcaaaa gctactgcga cgattatgca
    36781 gggattgcta cgagaagttc tatcttctcg tgtgacaaca accttcaagt ctaacctgac
    36841 ttctttaaat cctactctgg ctaatgcaga ttggattggg aagactggta caaccaacca
    36901 agacgaaaat atgtggctca tgctttcgac acctagatta accctaggtg gctggattgg
    36961 gcatgatgat aatcattcat tgtcacgtag agcaggttat tctaataact ctaattacat
    37021 ggctcatctg gtaaatgcga ttcagcaagc ttccccaagc atttggggga acgagcgctt
    37081 tgctttagat cctagtgtag tgaaatcgga agtcttgaaa tcaacaggtc aaaaaccagg
    37141 gaaggtttct gttgaaggaa aagaagtaga ggtcacaggt tcgactgtta ccagctattg
    37201 ggctaataag tcaggagcgc cagcgacaag ttatcgcttt gctattggcg gaagtgatgc
    37261 ggattatcag aatgcttggt ctagtattgt ggggagtcta ccaactccat ccagctccag
    37321 cagttcaagt agtagttcta gcgatagcag taactcaagt actacacgac cttcttcttc
    37381 aagggcgaga cgataattct ctaaatgaag tggccaatca atggattgcc acttttttcc
    37441 tttcgtgtta caataagggt atgaatcagt atcagaaaaa gattgttaac ggaaaaattt
    37501 attcgctcct atccggctta atatggggaa tctgtggaat tttaggagag tacttcttta
    37561 ctcattatca ggtgtcttcg ggctggatta cctctatgcg tttgacactg gcagggagtc
    37621 ttgtactcat ttggtctgca atacaattaa aatcgcaagt gctagatatt tggcgagaca
    37681 agaaaaatta cctgcccttt ttagcctatg ctattttggg gattttttca gttcagtatt
    37741 ttttctatct ctgtgtagaa tactcaaatg ctacgacagc aactatttta cagtttatta
    37801 gccctgtctt tatcctcttt tacaatcgct tggtttatca aaaacgagcg tcaaaaagcg
    37861 ctgttttcta tgttttggtt gccatgctgg gtgtttgctt gatggcgaca aagggagatc
    37921 tctctcagtt atccatgacg ccgctagctc ttataacagg tttgctgagt gccatgggtg
    37981 ttatgtttaa tgttatcttg ccccaacctt ttgctaagcg ttatggtttt gttcctacgg
    38041 ttgggtgggg gatgattttg gcaggtttgt ttagcaatgt cctctcgccg gtttatcagc
    38101 tttcctttac tcttgatatt tggagtatct tgatttgcct cattatcgct ttctttggaa
    38161 cggcttttgc ttttttcatt tccatgaagg ctgtgtcctt ggtttctcct ttggtggttt
    38221 ccgttatcag tgccagtgaa cctctctctt ctgctctctt gagtgttttg ttcttaggat
    38281 tagtagtgga ttggtccctc cttctagcta tagtcttgat tattttaccc atgatatttt
    38341 tgtctataga agaagcgaaa gaaagtagat aaaaagtctt ttcttaattc taaaagtgtg
    38401 ctatactaga atagtcaata aaacacgggg agatagttgt gaagagtgtt tttaggttgt
    38461 atcggtaggg gcttgctttt accgacagca cgttttatct cacaacccta aaaaatcata
    38521 cctaaaaaca gacaaaaagg tttcaaattt gaggcctttt ttggtagaat aggtatcatt
    38581 ataacgaacc aggaggcacc tatgactgct acaaaaatga acgctcaaga aattatccaa
    38641 tttatcgcca atgctgaaaa gaaaaccagt gtcaaagtaa cctttgaggg gcaactcgca
    38701 actgctgtgc ctagctctgt tgtcaaacta ggaaatgtcc tattcggaga ctggaaggac
    38761 gtggctccgc ttcttgaagg tttggtagaa aatcaagatt atgttgtcga gcaagatgct
    38821 cgtaattctg cagttccttt gctagataag cgtgctatca acgctcgtat cgagccaggt
    38881 gctattatcc gtgaccaagt ggaaattggt gacaatgctg ttatcatgat gggagctgtt
    38941 atcaatatcg gtgctgaaat cggtgctgga accatgattg acatgggtgc catccttggt
    39001 ggccgtgcca tcgttggaaa aaatagccac gttggtgcag gtgcagtttt ggcaggtgtg
    39061 attgagccag ctagtgctga accagtccgt gtcggagaca atgttcttat cggtgctaat
    39121 gcagtggtta tcgaaggagt ccaaatcggt agtggttcag ttgtcgcagc aggagctatt
    39181 gttacccaag atgtcccaga aaacgtggta gtagcaggtg ttccagctcg tattatcaaa
    39241 gaaattgatg cccaaactca acaaaaaaca gcgctagagg atgcgcttcg taccttgtaa
    39301 ttgtaaaagt aaaaaagagg cggaaccctt tttccagcct ctttctgcta tatcggagga
    39361 cagatagatg ttagatttga ttcagactag acgagattta caccagatac cagagattgg
    39421 cttggaggag ttcaagactc aggcttattt gctggatgtg attgagaaat tgactacggg
    39481 caaggatttt gttcaaattc gtacttggcg gacagggatt ttggtctacc tgcagggaag
    39541 tcagccggag cgaaccattg gttggcggac agatattgat ggcctgccta tcgtcgaaca
    39601 aacaggtcta ccttttgctt ctcaacacca aggtcgcatg catgcctgtg gtcatgattt
    39661 tcatatgacc attgccttag gctgtcttga gcgcgccctt gaggagcaac ccaagaataa
    39721 tctgctcttt ctatttcagc ctgctgaaga aaatgaagct ggtggtatgc tcatgtatga
    39781 ggacggtgct tttggagatt ggttgccaaa ccaattctat ggtctccatg ttcgtccaga
    39841 tttgaaagtc ggccagattg cgactaatac tcatacactc tttgcaggaa cttgcgaggt
    39901 gaagatccgt ttcaaaggca aaggtggcca cgcagctttt cctcatgaag ccaatgacgc
    39961 cttggtggcg gctagttact ttgtgaccca agtgcagtca gttgttagcc gcaatgtcaa
    40021 cccaatcgag ggagcagtgg tgaccttcgg cgtttttcaa gctggaacca ccaacaatgt
    40081 catcacagac acagcttttt tacatggaac tattcgtgcc ttgacacagg acatgagtct
    40141 cttggtgcaa aagagggtaa aaacagttgc agaaggggtt gcagctgcct ttgatatgga
    40201 agtcgaagtg gaactcaaac aaggtggata cctacctgtt gagaacaatc cagccttggc
    40261 gcgtgaactg atggacttct ttgacgagaa agacggaatc gagttgattg atatcgaacc
    40321 tgctatgaca ggcgaggact ttggttatct cctttcaaag gttgatggcg tgatgttttg
    40381 gctaggtatc gatagtccct acgctcttca tcaccctcaa atgagtccta aggaagaagt
    40441 cttagctatt ggggtggctg cggtgtctag tttcctgaaa aagaaggcag cagaatagag
    40501 ggcttgtcta tgaaatcgga attacgcaag caagtcttgc atgaaatgaa ggctttatct
    40561 caagaacaaa aacaggctat agaccaagct ttaaccgagc gaattttaca acaccccttt
    40621 tatcaagaag ccaaggtcat cgcaacctac ctctcttttt ctcatgagtt tcaaacgcgg
    40681 gaactgattg agcaggcgct gaaggacggc aagagggttc tgacacctaa gacctatccc
    40741 aaggggcgca tggactttgt ggtctatgat ccgcaacagt tggtaaaaac tgcctttgcc
    40801 ttactggagc cacagggaga tttggaagtg gtggatgtat ctcagattga tttgattcat
    40861 gttcctggcc tggcttttac gacggatgga tatcggattg gatatggtgg aggttattat
    40921 gaccgctatc tggaacattt ttctggtcat actttgagta cggttcatcc ttgtcaaatt
    40981 caggacttta tacctgaaaa ccatgatatt cctgttcagg aggtattaat tgatgaagga
    41041 aatctttgat agacgttacc ctgtgacgag tttcttcctc ttagtgacgg ccttggtatt
    41101 tttactaatg ttggtcactg caggcggaaa ctttgacagg gcagatacat tatttcgatt
    41161 tggagccatg tatgggccag ctattcgcct ctttcccgag caggtttggc gtctcttgtc
    41221 tgccattttt gttcatattg ggtgggaaca tttcattgtt aatatgcttt cactttatta
    41281 tcttggaagg caggtagagg agattttcgg ctctaagcag ttcttttttc tctatctctt
    41341 atcaggaatg atgggcaatc tctttgtttt tgtatttagt cctaaatcct tagcagcagg
    41401 cgcctctacc tctctttatg ggctatttgc cgcgattatc attcttcgct atgcaactcg
    41461 caatccttat atccaacagc tagggcaatc ctatctgaca ctttttgtgg ttaacattat
    41521 tggaagtgtt ctgattccag gaatcagcct agcaggccat atcggtggtg cagttggtgg
    41581 cgcatttcta gcagttatct ttccagttag aggagaaaaa cggatgtata acaccagcca
    41641 gagattagga gcggtagtct tgttcgtagg actcgccatt ttgcttttct acaagggaat
    41701 gggaatgtga cgaagttgaa agaaaatttg tgtaatgaaa aaagataagg cattttttga
    41761 accttatctt tttattttat tccttctcag tcaattcttt tccaagttgg atgaggtaat
    41821 tcttcaaatc atctttgact tgtggatgtt tgagggcgta gtcaatagat gttttcataa
    41881 agccaaactt atccccaacg tcgtaacgag ctcctgtaaa ttcacgggca aaaacacgct
    41941 gtgttttatt aagagtatcg attgcatcag taagttggat ttcatttcca gcaccaggag
    42001 cttggttttc gaggatttgg aaaatttcag gcgtgaggag gtagcgtccg ataatagcaa
    42061 ggtcgctagg agcgtcctct ggagctggtt tttcaacaaa ggtttcaaca ctgtaaagac
    42121 catcttttcc ttcgccttgc ggagcaataa ccccgtaagc agatacttcg tcatgaggga
    42181 ctggcatgac agcgatagta gacgcgtggg tacgcttgta gtcatccatg agttgtttgg
    42241 taagtggaac agccttttcg tctgtgatat ccatcaagtc atcaccaagc ataacgacaa
    42301 aaggttcatt tccgacgaaa gccttggctt gcaaaacagc atctccgaga ccgcgtggat
    42361 gagtttggcg gataaaatgc agacgcatgc cagttgtttc atcaactagc ttcaaaagat
    42421 ctgttttccc tttttctttg aggttatatt ccaattcgaa gtttgaatcg aagtggtcct
    42481 caatagaacg ttttgattta ccagtgacaa ctagaatatc ttcaatacct gatttgagag
    42541 cctcttccac gataaactgg atagttggtt tgtctacgat tggcaacatt tctttggcaa
    42601 gggccttggt tgctggtaaa aatcgagttc ctagtccagc agcagggata actgcctttc
    42661 taacttttga tgtcataaaa atcctttcta tagagggtta agaccactca ttttctgctt
    42721 taaattcatt gttcatgatg tcataaatgg catctttgat attggttccg tgataaataa
    42781 cttggtaaat agcctgtgta atgggcatat agactccaag ttcttgggct agttcatagg
    42841 ctgctcgagt cgttgaaatt ccttcgatta ccatgcccat attagcttct atatcagcta
    42901 gggattctcc tcgtccgaga gcatctccag ctctccagtt acgagagtgg atggaagttc
    42961 ccgttacgat caaatctccc acaccagata agccgctata ggtcaatgga ctggccccga
    43021 gtgctacccc taggcgggtg atttctgcta aacctcgagc gatgatggct gccttagcat
    43081 tatcaccaaa tccaagacca tgtaaagctc cagcaccgac agcaataata tttttaagag
    43141 caccagcagt ttcaaccccg ataacatccg tattggtata aagtcggaag tagtgattac
    43201 taaatagctc ctgaacgtat tgagctgttt gtaaatcttt agaagcagca gttattaaag
    43261 ttaggtcacg cacaatggtc tcttctgcat gactaggccc tgaaacaacg acgatatcac
    43321 tacggagatg ttcaggaatt tcttcttcaa gaatggttga taatcgttta tggctatcag
    43381 gttctaatcc ctttgatgcg tgcatgatga taaccttatg gtccaaggtt tgtgcaactt
    43441 gctgggcaac aagtcgtgtc acttttgttg ggacaacaaa caaaatcgca tccacatttt
    43501 tcaatgtttc tgctaagtcg gtgtaggcaa tgatattttc gtctagaacg acatctttaa
    43561 agtagtgctt attagtatgg tgtgtattaa tttcattgat ttgctcggga agatttcccc
    43621 aaatacgtac ctcgtgtcca ttgtcattta agacttgtga aagggcggtt ccccaagaac
    43681 caggccccaa gacggcgacg gtttgttttt ccatgatttc ctccttgata gatttctttc
    43741 tcttattcta tcatattcta ggggattttg cacttgcatt actggggtat ggtggaaaaa
    43801 tacacaaaga ccgagatgaa gggataaact cttagtcttt agttaaagtg agaagaaatc
    43861 tgatagatag tttgattcag tatatgctta taatagaggt aaacaactca ggaagttctt
    43921 gtaggttgcg agagtttggc gaaaaaatta aaagattacg tttggctaaa aaaatcagtc
    43981 gttcagaatt ttgtggtgat gagtctgaat taagtatccg tcaattaatt agaattgaaa
    44041 atggagaatc cagaccaaca ctaacaaagt taaaatatat tgctgaacgt ttggaggttg
    44101 aagattacaa gttgatgcca agttatatag agttggataa ggaataccta gaattgaagt
    44161 atttcttgat gaggactcct acatacgaag atgaaactat cgcccaaaag aaagagagtg
    44221 tttttgataa gatttttgaa gagtattatg ataggctacc tgaggaagaa agatttatca
    44281 tcccaaatta ttcatatcta gcactagcga actacacagt tcaaaaatta ccagaaaagc
    44341 tagttgaaat actgtccttc tggtgatttt gcttaatttc tgatgttcga gacatgattg
    44401 aggagatttg tcttgatttt ttcctaaaaa agggcagact cctcccttgg tttgatgctc
    44461 gatttttaaa ataaggtgac ttattctgtt aggcagtttt cccctgccct ctagctctat
    44521 atggaacagg gagtatcaga tttatctggc agattgtatc aaataaggct tgaaactgtt
    44581 ttgaataggc gtatagactt gagaatttga gaatatgtcg tctagcagta gagacatatt
    44641 ttccggcaat atgaaggaag agacgtcgaa aacgcttgat agtcaaggcc tttacttcat
    44701 caccagctag ctgctttaaa aagaggtaga gattataagc cagacagccc atcatcatac
    44761 gtacttcatt cttaatcatg gtcgaactat ctgtcttatc cccaaagaat cctgctttcc
    44821 tttctttgat aaagttttca gcattccctc gttcacgata gaattgaaac agctcctcac
    44881 tatctacact gcttaaattg gtcaaatgaa cttttttaca accataaaaa agagcactta
    44941 atcgtactct tctcttttaa ggattagttg aattaattca aatccactag ttctcctgtt
    45001 tctaggtaga ctaggcgttc acaaatgtta gcaatgtaat cagcgaagcg ttccagatgc
    45061 cctatgatat aaaggtattg agttccattg ggaattgagg tttcttggtc tttcataagt
    45121 ccaatgattt cctttgataa ggcataataa tattggtcaa tctgttcatc tttttgagca
    45181 atactaatag cttttgaggc ttggtgcaaa ggaaaggcaa ccaataaatc ggctagcatg
    45241 ctgagggata atttacccat ttggtgtaac tgttcttcgt caggggctag ttgattttct
    45301 tttagttgca aaacagcttt ggcaatgcct gccatatggt ctcccatacg ttcaaggtct
    45361 gaacaagaag acatgatgct aatcacaaat cgaaggtcag acacttgtgg ctgctgcaag
    45421 gccaacaaac gggcacaggt caattcgata gcgctttgac cttggttgat agcatgatcc
    45481 ttattgataa ttagctctgc catctccttg tctttggagg ctaaggccag taaggttttt
    45541 gaagctgttt caaggacaag ttgccctagt cctaaaaagg attgttctaa ttcatgcaat
    45601 tctaagtcaa attgatttct cataggtttt tccttcctta tccgaaccgt cctgaaatat
    45661 agtcttctgt gcgctgatct tttggattgg taaacacgtc aacggtgtct ccaaattcgc
    45721 aaatttctcc tgttaagaaa aaagcagttt tatctgaaat acgtgaagct tgttgcatgt
    45781 tatgggtaac aatgataatc gtataatcct tttttagttg ctgaatgagg tcttcaattt
    45841 ttaaagtgga gatagggtct aaggctgaag tcggctcatc cattaacaga atatcaggtt
    45901 ctactgctaa agctcgcgca atgcaaaggc gttgctgctg accgccagat aaggacatgg
    45961 cactcttttt aagatcatct ttgacttctt cccaaatggc tgcccctttt aaagatttct
    46021 ccactaaggc atctaattgt tttttgtctc gaataccatg tgtccttggg ccataagcca
    46081 cgttatcata gatagacatg gcaaagggat taggctgttg aaaaaccatc cctacacgct
    46141 tacgtagctg attaaggttg aatttgctac tataaatatc ttgctcatct aagaggactt
    46201 ggccttcaat atggcaagaa ggaaccaaat cgttcatccg attaagggtt tttagaaaag
    46261 ttgatttgcc acaaccagat gggcctatca aggcagtaat ctgtctttct ggtaattgaa
    46321 tcgaaatatt ttttaaggct tgaaaatccc cgtaaaataa gtctaggtgt ctaactgaaa
    46381 atgttcccat actaggaagc tcctttcaca agtttccgag ataataagct tgatagagta
    46441 tttatcatta aaacagtaat aatcaaaatc acgccggtag cataggcttc attgacatgt
    46501 agcccctcac ttgacagcat atacatatgt agggctagag aacggcctga agacatgaga
    46561 ctacttggcg tattggtaga ggtacctaat gtatacatga gggcagctgt ttcaccaacg
    46621 atacggccaa tagctagtat cactccagct aaaatacctg gcatggcaac tggtagaaca
    46681 attctaaaaa cagtccgtaa cttacctgcc ccaagtccat aacttgcttg acgcatgcta
    46741 tcactaacag ataaaagggc ttcttctgtt gagcgaataa tgactggcaa caccatgata
    46801 actgaggtta agattcctga taacagagag tattgaaaac ctaagaagac tacaaagaag
    46861 agcatgccaa acagaccaaa aacaatggaa ggaatcccag acaaggtatc tgaggccaat
    46921 cgcatgattt taacacaaag ggaatctttt tttgtatatt ccacaagata aaaaccagca
    46981 aaaatcccta tgggcaaggc taaaagaaga gcaccaaaaa ccagaataac ggtggaaata
    47041 atcgctggca taagggaaat gttctcagaa gtataagtcc aagaaaagag ggatagactt
    47101 agatgaggta agcctttgat gaggataaaa ccaatgatta aaaagagaga gccaaaggtt
    47161 aaagctgaaa aacaataaac gagaagtttt agcaggtatt tactcataag atgattttcc
    47221 tttcaagtag gcaaagtagg cattaatcaa gagaataagg aaaaagagaa ctgctgaggt
    47281 tgcaataagg gcttccctat gctgacctga tgcgtaagcc atttccagaa caatattggt
    47341 tgttaaggtt ctggttcctg aaaagagtcc acttggaata atcggctggt tgcctgccac
    47401 caaaataact gccatggttt cacctactgc gcgaccgatt cctaaaataa ctgctgataa
    47461 aataccagat ctcgcagctg gcaagatgac actaaaaata ctccgttcat gactagctcc
    47521 tagagccaag ctaccagaat aatacgtttt gggaactgtt cggatagcag attctgacaa
    47581 actgataatg gttggcaaaa tcattattcc taatagtaac gaagcggtta ggacactcat
    47641 gccatttcct aaaaagcttc taatccaagg caccaataat tgtaggccga aaaaaccata
    47701 aacaatagat ggaatggccg ccatcaagtt gatagctgat tttaagaagc catagacggg
    47761 ctttggacaa taataaacca taaacaccga tgtcaagatg cctgttggca ccccaatcac
    47821 aatcgctcct aaggtaatta ataaggaacc aacgatcatt ggtaaaatac catagcttgc
    47881 cggaatgttc gttggcgacc aatcactgcc taataaaaaa cgggcaaagc cgtagttagc
    47941 catgaaaggt aagccattac taaaaataaa gaaacagatt agcaaaatag ctacaacagc
    48001 tactgttgca ctcatgaaaa aaattgccct aaaaactgct tctttgaagg cttgttttgt
    48061 cacatcttgt cctttctagt gaagaaagta agggagatac gacacctccc tacttgcctt
    48121 ctttatctta ttgtacgatg aaacgtctgc atctctttag agatttatgg agcaaacatt
    48181 ttatttaatc ttgtcccagg tggttaattt gccactaaaa acgtctgcaa gttcagccat
    48241 actgacttgg cttgccttat tgtcattatt gaccacaaca gcaataccgt ctaaagcaat
    48301 agcatcatgg gtgagactct taccttcttc aggagttaat tccctagaaa ccataccaat
    48361 atcagcggtt ttctccttaa cagcggtaat acctgctgaa gacccattag aggtaatatc
    48421 aatcgtaact tctggatttt cttttttata agcttctgct aatttttcca ttaaagaaga
    48481 tactgaagtg gaacctacaa cagacaactt gcctgataag tgttggcttg tatattctgt
    48541 ggtttcagtt ttagcttcaa taaatttatt atctgtgacc acttgttgac cttgtttgga
    48601 gtggataaag ctgataaaat cttgacctag cttggaaaga ttagaagacc aaacaatgtt
    48661 gaagggacgt tgaagagggt attcaccatc taaaactgtg tctcgactag ccttgacacc
    48721 atcaatctct aaagccttga cagatttcgt taaagatccc aaggagatgt agccgatagc
    48781 attagcattc ccttgaactg ctgagagaac accttctgta ctattttgaa tcacagctgt
    48841 tttggcagtg ttgtcaattt ttttatcacc gtcttttttg agaatccctg tgatttctgt
    48901 gaaggcaccc cgtgttccag agccattttc tcgtgaaatc acctcaatcg ttcctgaagc
    48961 tgactgtttg gaagcagctg actgattgcc acaggcaaca agcccaaatc ctgataagcc
    49021 aatggctgca agagtaagca tttttttgaa tttcataata atcaccttta tctctatgta
    49081 tttttcttgt gtaggcttac tacatttata gtctaacaag tctttgtaaa ggtttatccc
    49141 tgattcatgt aaagattgtg taaagaatca aaaaaagcca cttttgaaaa atggctgctc
    49201 ctaaaaatag ctttaaaaat tattagtcct gtgcgaaaga ttggttagga agaaaaatcg
    49261 tgaagcaact gcctctgcca agctgactcg tcaccgtgac ttggccacct aataattgac
    49321 tgagttcttt gacaatggca aggccaagac cagtgccacc agtttgtctg cttcgacctt
    49381 tattaactcg gtaaaaacgt tcaaaaatac gatcctgctc taattgacta ataccaatcc
    49441 ctgtatctga tacagaaatc ttaatgcctt cgttcacctt ttgggtcttg acctcaattt
    49501 ttcccccttg ttcagtgtaa cggatggcat tggataaaag attgagtaag atttgggaaa
    49561 gtaattgact atctgatacg agggtgacat catctggcac ctgcaccttt agctgtaaat
    49621 ccttcttctt gagctgaggt tgcaagcttt gagtcaaatc ttgtacaaat tctgccaaag
    49681 aaagggtcgt ccattgtata ggcatttgtt gagccttaga taaggtaaga agatgctcaa
    49741 caatatgctc aagacgcaaa ctttctttgt aaataatgtc tagaaagtca tccttgagcg
    49801 cttcttcttc agctgacatc cccttaatgg tttcagcaaa gcccttaatc gaagtaactg
    49861 gtgtcctcaa ttcatgggag gcatttgaga caaaggctaa atttaacttt tcataagttc
    49921 taatcgttgt taaatcatat agcaagacga gcacagcttc cacagattgg gtggggctaa
    49981 aaacgggaac tgctgtcact tctaaaatca agtcaccctc atgaaaccca cttacttctt
    50041 gttttaacct tgttttttga tcaaaggctt ggtgaactaa attccgaata tccatccgtt
    50101 tgaggtcatc aagtgaactt atgtcgccgt ccacatcggg aaaataatga ggcagagagc
    50161 gactggataa taacatctga ccttgagcgg aaactaaaaa cgtccccatg gttaggtgcg
    50221 acagaagaac ctccattgtt tcggctaggt ccttgtattg ctgatcctgt tgggagactt
    50281 tggtttttag gccagacaca tactgagcca aagactttaa gtcttcttgc cctttttcta
    50341 aaaagtattc actactggtc aagagaggtt ggtgcaaggt ctcaaaagca acttcccatt
    50401 tccaaaggca aaagagccag tagccaccta gtcccaaaga aagggctaga agaaagagac
    50461 cgatgccttt actgatccaa gttaatgcca tccctgcaat cagaatgagg ctaacactta
    50521 gattgactag ccaaaattga aggtagcgtt tcatctataa ctccttgaac ttataaccat
    50581 aaccccgaat ggttcgaata aattgagggg ctttaggatt gtcttcaatt ttttccctca
    50641 acttaccaat atgaacgtcc accaaacgtg tttcctgccc aaagtcatac ccccagatac
    50701 gttccaaaag acgctctcta gtcagtgtca tgttgggatg tttcataaga tagagcaaga
    50761 gttcaaattc ttttggggtc aaactcagta acttattcgc cttgtagact tcatgacgct
    50821 cagggtatac tttcaaggtc ccaaatagcc aagaatcgtc agcgatatta tctgaatcat
    50881 ctccttcttg ttctccttta gttcgcctga ggacagcctt gacacgcgcc agcaattctc
    50941 tagggctaaa aggcttggtc aggtagtcat cagcccctaa ttccaaggcc aaaaccttat
    51001 caaattcatc acttttcgca gaaaccatca taattggagt tttgacgcct ttggctctca
    51061 gccgcttaca aacttccatg ccatctaatt gtggtaacat gatatcaagc aagataaaat
    51121 caaagggttc tgtttctgcc aaagctaagg ccttccgtcc atttgttacc aattgagtag
    51181 aaaagccttc cttacttaaa tggtagtcaa gtaatttcag aatgtgttct tcatcatcca
    51241 ctaataagac ttgttttgtc atctattatc tcctattggt aacattataa cacaattatc
    51301 agaaatccta acattgctaa atcagattaa atttgcttat caagactagt atctggtcaa
    51361 acgctcaatc atctccttgt gctctggata ggtcgccagt agatctaccc tttcaaataa
    51421 ttcaaaatcc tcaaattcaa aaccaggagc aacaagacaa gaaaccagag catcatcctt
    51481 atcaactgtt gatccccaaa tagtgccctt aggaacacag tagtgaagtt gttgcccttt
    51541 ggatatgtcc aggcctaaag tgactacttc gtagtgacca tctgctgtaa tcatgtgaac
    51601 agtaagtggg gatcctgcat gaaaatacca gatttcatct gctgtcaatc ggtgaaaatg
    51661 tgaaggattc gtttcttcta ataagaaata aatactggta taaagcgccc ttcccttacc
    51721 agcaaggttt atagtgtctg aagctttttt tgtttgtcta aaatagccac cttcaacatg
    51781 gggagctaac tctagagttc ttatcaagtc ttctttatcc atcggggcca atgggttgaa
    51841 gtaactcttg ttcaaagtgg ttttacgatt tcaagaactc ctctcagttc tgaggacacg
    51901 gtaatgattg atgcgacgga agtaaaaatc aatcgctcta aaaaaagaat tagcgaatga
    51961 ttttggtaaa aagaaatttc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
    52021 aattgtttct ttggatatcg ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag
    52081 tcgcagaaat atcggacaag ctggaaaaat cttggctgac agtggttatc aagggctcat
    52141 gaagatatat cctcaagcac aaactccacg taaagccagc aaactcaagc cgctaacagc
    52201 tgaagataaa gcctataacc atgcgctatc caaggagaga agcaaggttg agaacatctt
    52261 tgccaaagta aaaacgttta aaatattttc aacaacctat cgaaatcatc gtaaacgctt
    52321 cggattacga atgaatttga gtgctggtat tatcaatcat gaactaggat tctagttttg
    52381 caggaagtct attatttggt taggtgaatt agtgaagcgt ttaggcaagt gtctctggtt
    52441 acgacgtcat ggcctctaaa tcgattatat ttaggggtca tgactagtga agcagttagc
    52501 tagttcgcat ataagcggct agcgtctaac aattaggaac tttagttcca ataactttaa
    52561 gattacgacg ttttaggaca taaatcgatc atatttatgt cctaaaacta gtgaagcgcc
    52621 tagccaaagt ccgaatagga tttggcgtta gttacttaga ttgctttgca atcaagtaac
    52681 tttggcgatt tacatcttct ctggcgcttc tactccaagc aagcgaaggg cttctttgag
    52741 aacgactgcg gttgcgtagc tgagggctag acggctgtcg cgttctgggc tttcatccaa
    52801 gatacgtgta tgtgcatagt atttgttaaa ggattgagcc aggctaattg caaatttagc
    52861 aatgatagaa ggttcaaagt tatctgccgc acggttgata atacgtggga agtcttgaat
    52921 gagtttaatg atttcccagc tttcagtatc attcaagcta tagttgccag ctgtttctgg
    52981 tttgaaatcg gctttgcgta agatagattg gatacgagcg taggcatatt gaacgtaagg
    53041 tccagtttca ccctcgaagg ataccatagc ttctaggtcg aagtcgtatc catttgtacg
    53101 gtcagttttg aggtcataga atttaatggc tccaacccca acagcatgtg ctacttggtc
    53161 tttgttttct agttcaggat ttttagcctc gatttggacc ttggcacggc taacagcctc
    53221 tgcaacagta ggctctagca agatgacatt ccctttacga gtagagagct tcttcccttc
    53281 ttttgtaacc agaccaaaag gaacgtgggt aatgtcgtca ctccaatcgt agcccatctc
    53341 ttgcaagaca gctttgagtt gtttaaagtg ggcagattgc tcttgaccaa cgacgtagat
    53401 agatttagca aattggtatt catttttacg gtagagggct gcagccaagt cacgtgtgat
    53461 atagagagtt gcaccatcag acttcttgat gagggctgga tgttcaattc catatttctc
    53521 aagattcaca acttgggcac cttctgattc aagaagtagt cctttttcag aaagaatgtc
    53581 tacaactgca tccatcttat cattgtagaa ggcttctccg ttatagctgt caaattcaac
    53641 cttcaattca ttgtaaaggc ggttaaattc cactaaactt tcatcgcgga accattgcca
    53701 aagagcgaga gcttcctcat ctccattttc aagtttacgg aaccattcgc gcgcttcttc
    53761 atccaagcta gggtcatttt cagcttcagc gttgatgcgg acatagagtt taaggagttc
    53821 atcgattgga tgagctttta cagcttcttc gtcgccccat tttttgtagg caacaatcaa
    53881 catcccaaat tgtttacccc agtctcccaa atggttgacc ttgaccgttt gataaccgat
    53941 tttttggaaa atatgtgaca agctatctcc gataacagtt gaacgcaggt ggccaataga
    54001 aaatggttta gcgatattcg gactagacat gtcgataaca acattttctt gtttgccaat
    54061 attttggtca gcatagtgtt ctttttcagt ggtaacagct tgcaatactt gagcagaaat
    54121 ggcagattta tcaaggaaaa agttaacgta aggtcctgtt gcgacaactt tttcaaaggc
    54181 ttggctgttc attttttcag ccagttcagc cgcaatcatt tgtggtgctt tacgttcgac
    54241 tttggcaaga gaaaaagcag ggaaagcgat gtcacccatt tctgagtttt taggggtttc
    54301 cagtaacttt aaaatagcct cttggtccag gctatcaatg atgctagcca attcgctagc
    54361 aatcaattct tttgtattca ttaagagctc ctttttggac ttttctacta ttttaacaca
    54421 attttaaaga aagaagaaaa aatttttgaa atctcctgtt tttttggtat aatatggtta
    54481 taaatatagt tataaatatg cacgcaagag gattttatga gaaaaagaga tcgtcatcag
    54541 ttaataaaaa aaatgattac tgaggagaaa ttaagtacac aaaaagaaat tcaagatcgg
    54601 ttggaggcgc acaatgtttg tgtgacgcag acaaccttgt ctcgtgattt gcgcgaaatc
    54661 ggcttgacca aggtcaagaa aaatgatatg gtgtattatg tactagtaaa tgagacagaa
    54721 aagattgatt tggtggaatt tttgtctcat catttagaag gtgttgcaag agcagagttt
    54781 accttggtgc ttcataccaa attgggagaa gcctctgttt tggcaaatat tgtagatgta
    54841 aacaaggatg aatggatttt aggaacagtt gctggtgcca ataccttatt ggttatttgt
    54901 cgagatcagc acgttgccaa actcatggaa gatcgtttgc tagatttgat gaaagataag
    54961 taaggtcttg ggagttgctc tcaagactta tttttgaaaa ggagagacag aaaatggcga
    55021 tagaaaagct atcacccggc atgcaacagt atgtggatat taaaaagcaa tatccagatg
    55081 cttttttgct ctttcggatg ggtgattttt atgaattatt ttatgaggat gcggtcaatg
    55141 ctgcgcagat tctggaaatt tccttaacga gtcgcaacaa gaatgccgac aatccgatcc
    55201 ctatggcggg tgttccctat cattctgccc aacagtatat cgatgtcttg attgagcagg
    55261 gttataaggt ggctatcgca gagcagatgg aagatcctaa acaagcagtt ggggttgtta
    55321 aacgagaggt tgttcaggtc attacgccag ggacagtggt cgatagcagt aagccggaca
    55381 gtcagaataa ttttttggtt tccatagact gcgaaggcaa tcaatttggc ctagcttata
    55441 tggatttggt gacgggtgac ttttatgtga caggtctttt ggatttcacg ctggtttgtg
    55501 gggaaatccg taacctcaag gctcgagaag tggtgttggg ttatgacttg tctgaggaag
    55561 aagaacaaat cctcagccgc cagatgaatc tggtactctc ttatgaaaaa gaaagctttg
    55621 aagaccttca tttattggat ttgcgactgg caacggtgga gcaaacggca tctagtaagc
    55681 tgctccagta tgttcatcgg actcagatga gggaattgaa ccacctcaaa cctgttatcc
    55741 gctacgaaat taaggatttc ttgcagatgg attatgcgac caaggctagt ctggatttgg
    55801 ttgagaatgc tcgctcaggt aagaaacaag gcagtctttt ctggcttttg gatgaaacca
    55861 aaacggctat ggggatgcgt ctcttgcgtt cttggattca tcgccccttg attgataagg
    55921 aacgaatcgt ccaacgtcaa gaagtagtgc aggtctttct cgaccatttc tttgagcgta
    55981 gtgacttgac agacagtctc aagggtgttt atgacattga gcgcttggct agtcgtgttt
    56041 cttttggcaa aaccaatcca aaggatctct tgcagttggc gactaccttg tctagtgtgc
    56101 cacggattcg tgcgatttta gaagggatgg agcaacctac tctagcctat ctcatcgcac
    56161 aactggatgc aatccctgag ttggagagtt tgattagcgc agcgattgct cctgaagctc
    56221 ctcatgtgat tacagatggg ggaattatcc ggactggatt tgatgagact ttagacaagt
    56281 atcgttgcgt tctcagagaa gggactagct ggattgctga gattgaggct aaggagcgag
    56341 aaaactctgg tatcagcacg ctcaagattg actacaataa aaaggatggc tactattttc
    56401 atgtgaccaa ttcgcaacta ggaaatgtgc cagcccactt tttccgcaag gcgacgctga
    56461 aaaactcaga acgctttgga accgaagaat tagcccgtat cgagggagat atgcttgagg
    56521 cgcgtgagaa gtcagccaac ctcgaatacg aaatatttat gcgcattcgt gaagaggtcg
    56581 gcaagtacat ccagcgttta caagctctag cccaaggaat tgcgacggtt gatgtcttac
    56641 agagtctggc ggttgtggct gaaacccagc atttgattcg acctgagttt ggtgacgatt
    56701 cacaaattga tatccggaaa gggcgccatg ctgtcgttga aaaggttatg ggggctcaga
    56761 cctatattcc aaatacgatt cagatggcag aagataccag tattcaactg gttacagggc
    56821 caaacatgag tgggaagtct acctatatgc gtcagttagc catgacggcg gttatggccc
    56881 agctgggttc ctatgttcct gctgaaagcg cccatttacc gatttttgat gcgattttta
    56941 cccgtatcgg agcagcagat gacttggttt cgggtcagtc aacctttatg gtggagatga
    57001 tggaggccaa taatgccatt tcgcatgcga ccaagaactc tctcattctc tttgatgaat
    57061 tgggacgtgg aactgcaact tatgacggga tggctcttgc tcagtccatc atcgaatata
    57121 tccatgagca catcggagct aagaccctct ttgcgaccca ctaccatgag ttgactagtc
    57181 tggagtctag tttacaacac ttggtcaatg tccacgtggc aactttggag caggatgggc
    57241 aggtcacctt ccttcacaag attgaaccgg gaccagctga taaatcctac ggtatccatg
    57301 ttgccaagat tgctggcttg ccagcagacc ttttagcaag ggcggataag attttgactc
    57361 agctagagaa tcaaggaaca gagagtcctc ctcccatgag acaaactagt gctgtcactg
    57421 aacagatttc actctttgat agggcagaag agcatcctat cctagcagaa ttagctaaac
    57481 tggatttgta taatatgaca cctatgcagg ttatgaatgt cttagtagag ttaaaacaga
    57541 aactataaaa ccaagactca ctagttaatc tagctgtatc aaggagactt ctttgacaat
    57601 tctccacttt tttgctagaa taacatcaca caaacagaat gaaaaggagc tgacgcattg
    57661 tcgctccctt ttgtctattt tttaaggaga aagtatgctg attcagaaaa taaaaaccta
    57721 caagtggcag gccttggctt cgctcctgat gacaggcttg atggttgcta gttcacttct
    57781 gcaaccgcgt tatctgcagg aagtattaga cgccctcctt actgggaaat atgaagctat
    57841 ttatagtatc ggggcttggt tgattggtgt ggccgtagtc ggtctagttg ctggtggact
    57901 caatgttgtc ctcgcagcct atattgccca aggagtttca tccgaccttc gggaggatgc
    57961 cttccgtaaa attcaaacct tttcttatgc tgatattgaa caatttaatg cgggaaatct
    58021 agtcgttcga atgacaaatg atatcaacca gattcagaac gttgtcatga tgaccttcca
    58081 aattcttttc agacttcccc tcttgttcat cggttcgttt atcctagcgg ttcaaacctt
    58141 accttctctg tggtgggtga ttgttctcat ggtagtcttg atttttggtt tgactgctgt
    58201 catgatggga atgatggggc ctcgttttgc caagtttcaa acccttcttg agcgcatcaa
    58261 tgccattgcc aaggaaaatt tacgtggcgt tcgtgtggtc aagtcctttg tccaagaaaa
    58321 agagcaattt gctaagttta cagaggtctc agacgagctt tttggtcaaa acctttacat
    58381 tggttatgcc ttttcagtag tggaaccctt tatgatgttg gttggttacg gggcggtctt
    58441 cctctctatt tggctggtcg cgggaatggt tcagtcggat ccgtctgttg ttggttccat
    58501 cgcttctttt gttaattacc taagccagat tatctttacc attgttatgg ttggattttt
    58561 gggaaattct gtcagccgtg ccatgatttc catgcgtcgt attcgagaaa ttcttgacgc
    58621 agagccagct atgaccttca aggatatccc agatgaagag ttggttggaa gccttagctt
    58681 tgaaaatgtg acctttacct atccaatgga caaggaaccg atgctgaaag atgtgagctt
    58741 tactattgaa cctggtcaaa tggttggtgt agttggagcg acgggtgcag gaaagtcaac
    58801 cttggctcaa ttgattccac gtctctttga tccacagaac ggggccatta aaatcggtgg
    58861 caaggatatt cgagaagtga gtgaaggaac cctgcgtaaa acagtttcca tcgttctcca
    58921 acgtgccatt ctttttagtg gaacgattgc agataacttg agacagggga agggaaatgc
    58981 tactctattt gaaatggagc gcgcagccaa tattgcccag gctagtgaat tcattcatcg
    59041 tatggagaaa acctttgaaa gtccagttga agaacgggga accaatttct ctggtggaca
    59101 aaaacaaagg atgtcgattg cgcgtgggat tgtcagcaat ccacgtattc tgatttttga
    59161 cgattcgacc tcagccttgg atgccaaatc agagcgcttg gtgcaagaag ctttgaataa
    59221 ggacttgaag gggacgacaa ccattattat tgctcaaaaa attagctcgg ttgtccatgc
    59281 agacaagatc ttggttctaa atcaaggacg attgattggt caaggcacgc atgcagactt
    59341 ggttgccaac aatgccgttt accgtgaaat ctatgaaaca cagaaatgaa agacaaacta
    59401 taagaaaagt caatagtttt atctaaatta tttcttattt caatttgatg atttggcgat
    59461 gattttagag cacggcaaaa agcccttgaa aaagtccatt ttttcaaagg taatcctgtg
    59521 ttaatttcag aaattacatc actttttgtt cgtcaaatgg cagctctttt ttaggatata
    59581 aaacagggtt cggataagtt tttttgcaag gtggatgatg gctacattgt aatgttttcc
    59641 ttgttctaat ttagtcttaa gataggcctt aaaagcaggc gaaaagcgag ggcatgcttt
    59701 ggcagcttgt atgagtacct accgcagatg aggggaaccc cgtttgacca ttcttccagc
    59761 taaatcaatc tgacctgact gataaataga agaatccagt ccagcgaaag cttgtaattg
    59821 agcaggatta tcaaaggcat gaatatttcg aatctcggct aaaatgaccg cccctaaacg
    59881 attcccaatc ccagtaaccg tcgttatgat cgagttgaac tcagccatca agtcattgac
    59941 acatgtttcc gccttgtcaa tgagcctctt gtaatgtttg atgttttcat tacacgagat
    60001 aaaacgttta tgcgttatca aactcatgac caattgaaac aaaaagctgt ggttagatcc
    60061 tttcggaaat tgtcaagcga ttggaggaaa tgaactaatc cacagcggct tattccaagt
    60121 ataccacttg ggctttggca gtagctaact gcgctaaata taatataagg aggagtaaaa
    60181 tgaagacagt tcaatttttt tggcattatt ttaaggtcta caagttctca tttgtagttg
    60241 tcatcctgat gattgttctg gcgacttttg cccaagccct ctttccagtc ttttctggac
    60301 aagcggtgac gcagctagcc aatttagttc aagcttatca aaatggcaat ccagaacttg
    60361 tatggcaaag cctatcagga atcatggtca atcttggcct gctggttttg gttctattta
    60421 tctctagtgt aatatacatg tgtctcatga cgcgcgtgat tgcagaatcg accaacgaga
    60481 tgcgcaaagg cctctttggt aagcttgctc agttgacggt ttctttcttt gaccgtcgac
    60541 aagatggcga tatcctgtct cattttacca gtgatttgga taatatccta caagccttta
    60601 acgaaagctt gattcaggtc atgagcaata ttgttttata cattggtctg attcttgtca
    60661 tgttttcgag aaatgtgacg ctggctctca tcaccattgc cagcacccca ttggctttcc
    60721 ttatgctgat tttcatcgtg aaaatggcac gcaaatacac caacctccag cagaaagagg
    60781 tagggaagct caacgcctat atggatgaga gcatctcagg ccaaaaagcc gtgattgtgc
    60841 aaggaattca agaggatatg atggcaggat ttcttgaaca aaatgagcgc gtgcgcaagg
    60901 caacctttaa aggaagaatg ttctcaggaa ttcttttccc tgtcatgaat gggatgagcc
    60961 tgattaatac agccatcgtc atctttgctg gttcggctgt acttttgaat gataagtcta
    61021 ttgaaacaag tacagcccta ggtttgattg ttatgtttgc acaattttca cagcagtact
    61081 accagcctat tatccaagtt gcagcgagtt ggggaagcct tcagttggcc tttactggag
    61141 ctgaacgaat tcaggaaatg tttgatgcag aggaggaaat ccgacctgaa aaggctccaa
    61201 ccttcactaa gttgcaagaa agtgttgaaa tcagtcatat cgatttttca tacttgcctg
    61261 ataaacctat tttgaaagat gtcagcattt ctgcccctaa aggccagatg acagcagttg
    61321 ttgggccgac aggttcagga aaaacgacta ttatgaacct catcaatcgc ttttatgatg
    61381 ttgatgctgg tggtatttat tttgatggta aagacattcg tggctatgac ttagatagtc
    61441 ttagaagcaa ggtgggaatt gtattgcaag attcggtctt gtttagcgga acgattagag
    61501 acaatatccg atttggtgtg ccagatgcta gtcaggaaat ggttgaggta gcagcaaaag
    61561 caacccacat tcacgactat atcgaaagtt tgcctgataa gtacgatact cttattgatg
    61621 atgaccagag catcttttca acagggcaga agcaattgat ttcaatcgct cgaaccctga
    61681 tgacagatcc agaaattctc attctcgatg aagcaacttc aaacgtagat acggtgacag
    61741 aaagcaagat tcagcatgcc atggaggtgg ttgtagcagg tagaactagt ttcgtcattg
    61801 cccaccgctt gaaaaccatt ctcaatgcag atcagattat tgtccttaaa gatggagaag
    61861 tcattgaacg tggtaaccac catgaacttt tgaagctagg tggcttttat tcagaactct
    61921 atcacaatca atttgttttc gaatgagaaa gaagttgtcc tatgtgggca gctttttctt
    61981 gtccataaaa aatgtttatc acagccttaa aaaaaacata ttagacgaaa gtcattttga
    62041 gtgatatgat aggattatcg ttagcattcg aaaggagagg catcatggct agaacggttg
    62101 taggagttgc tgcaaatcta tgtcccgtag acgcagaagg caaaatcatt cattcatctg
    62161 tatcttgtag attcgcagag atcattcgtc aagtcggtgg tctcccttta gtcattcctg
    62221 ttggtgatga gtcagttgta cgtgattatg tggaaatgat tgacaaactc attttgacag
    62281 gaggccaaaa tgttcatcct cagttttatg gagagaaaaa gaccgtcgag agcgatgatt
    62341 acaatctggt ccgtgacgaa tttgaattgg cactcttgaa ggaagcgctt cgtcagaata
    62401 aaccaattat ggcaatctgt cgcggtgtcc aacttgtcaa tgttgccttt ggtggaaccc
    62461 tcaatcaaga aatcgaaggt cactggcaag gactaccttt cgggacatct cactctattg
    62521 agacagtaga aggaagcgtg gtggctaagc tatttgggaa agaaagtcag gtcaactcag
    62581 ttcatcgtca aagcattaaa gatttggcac ctaatttccg tgtaactgct attgattcaa
    62641 gagaccaaac tattgaagcg attgagtcta tcgacgagca ccgcattatc ggtttacagt
    62701 ggcatccaga gtttctggtt aatgaagaag atggcaattt agaattattt gagtatttat
    62761 tgaatgaact gtaacgattg gaacatctgg tcgttctttc ttttattttt tcaaaatttt
    62821 tagaatgtgg cattttacgc aaacgtttga attctgataa aaattggaga aattcgacaa
    62881 aaaaacttga aaaaaacgaa ggtaagcgtt atgatagaaa agaagaaata ttggaggaag
    62941 aacatgtcac atattaaatt tgattattca aaagttttag acaaatttgt tgcaccacat
    63001 gaagtggaat acatgcaatc acaagtaaca gcagcagatg aattgatccg taaaggaact
    63061 ggtgctggta gcgacttctt gggatggttg gaccttcctg aaaaatacga ccgcgaagaa
    63121 tttgaccgca tcttgaaagc tgctgagcaa atcaagtcag acagcgatgt tttggttgtg
    63181 atcggtattg gtggatctta ccttggtgca aaagcagcaa tcgacttctt gaatcaccat
    63241 tttgctaact tgcaaacaaa agaagaacgc aaagctccac aaatccttta cgcaggaaac
    63301 tcaatctcat ctacttacct tgctgacttg gtagagtatg ttgcagacaa agacttctca
    63361 gtaaacgtaa tttctaaatc aggtacaaca actgaaccag cgattgcttt ccgtgtcttt
    63421 aaagaactct tggttaagaa atacggtcaa gaagaagcta acaaacgtat ctatgcaaca
    63481 actgaccgcc aaaagggtgc tgttaaggtt gaagcagacg ctaacggttg gggaacattt
    63541 gttgttccag atgatatcgg tggacgcttc tcagtattga cagccgttgg tttgcttcca
    63601 atcgcagcat caggagctga cataaaagct cttatggaag gtgcgaatgc agctcgcaaa
    63661 gactacactt cagacaaaat ctctgaaaac gaagcttacc aatacgcagc tgttcgtaac
    63721 atcctttatc gtaaaggcta tgcaactgag atcttggtaa actatgagcc atcacttcaa
    63781 tacttctcag aatggtggaa acaattggct ggtgaatcag aaggaaaaga ccaaaaaggt
    63841 atctacccaa cttcagccaa cttctcaact gacttgcact cacttggtca atttatccaa
    63901 gaaggaactc gtatcatgtt tgaaacagtt gtccgtgttg acaaacctcg taaaaacgtg
    63961 cttattccta ctttggaaga agaccttgac ggacttggtt accttcaagg aaaagacgtt
    64021 gactttgtaa acaaaaaagc aactgacggt gttcttcttg cccacacaga tggtgatgta
    64081 ccaaacatgt atgtgactct tccagagcaa gacgctttca ctcttggtta cactatctac
    64141 ttcttcgaat tggcaattgc cctttcaggt tacttgaatg ctatcaaccc atttgaccaa
    64201 ccaggtgttg aagcttataa acgtaacatg tttgcccttc ttggaaaacc aggatttgaa
    64261 gaattgagca aagaacttaa cgcacgtcta taatagaaga aaagagtggt ttgcccactc
    64321 tttttactct ctttatccat agaaattgga ctcagccaag acttgtgata taattataga
    64381 aagcaaaaag gcagacgcct agataatagg agaaactatg tcaaaagata tccgcgtacg
    64441 ttacgcacca agtccaacag gactactaca catcggaaat gctcgtacag cattgtttaa
    64501 ttacttgtat gcgcgccatc atggtggaac atttctcatc cgtatcgaag atactgaccg
    64561 taaacgccat gtcgaggatg gtgaacgttc acaacttgaa aaccttcgct ggttaggcat
    64621 ggattgggat gaaagtccag aatcacatga gaattatcgc cagtctgagc gtttggactt
    64681 gtatcaaaaa tatattgacc aactattagc tgaaggaaaa gcctataaat cttacgttac
    64741 agaagaagag ttggcagctg aacgcgaacg ccaagaagta gctggcgaaa caccacgcta
    64801 catcaatgaa taccttggta tgagtgaaga agaaaaagca gcttacatcg cagaacgtga
    64861 agcagtaggg atcatcccaa ctgttcgttt ggctgtcaat gagtcaggta tctacaagtg
    64921 gcatgatatg gtcaaaggcg atatcgaatt tgaaggtggc aatatcggtg gtgactgggt
    64981 tatccaaaag aaagacggtt acccaactta caactttgcc gttgttatcg atgaccacga
    65041 tatgcaaatc tctcatgtta tccgtggaga tgaccatatt gctaatacac caaaacagct
    65101 tatggtctat gaagctcttg gttgggaagc tccagagttc ggtcacatga ccttgattat
    65161 caactctgag actggtaaga agttatctaa acgtgatact aatacacttc aatttatcga
    65221 agattaccga aagaaaggtt accttccaga agcagtcttt aactttattg ctcttcttgg
    65281 ttggaaccca ggtggtgaag atgagatttt ctctcgtgaa gaattcatta aactttttga
    65341 tgaaaaccgc ctcagcaagt caccagcagc ctttgatcag aaaaaactag actggatgag
    65401 caatgattat atcaagaatg cagaccttga aaccatcttt gaaatggcaa aaccattctt
    65461 agaggaagca ggccgtttga ctgacaaggc tgaaaaatta gttgagctct ataaaccaca
    65521 aatgaaatca gtagatgaga ttatcccatt gacagatctt ttcttctcag atttcccaga
    65581 attgacagaa gcagagcgcg aagtcatgac gggtgaaaca gttccaacag ttcttgaagc
    65641 attcaaagca aaacttgaag cgatgacaga tgatgaattt gtgacagaaa atatcttccc
    65701 acaaattaaa gcagtccaaa aagaaacagg tattaaaggg aaaaatcttt tcatgcctat
    65761 tcgtatcgca gtttcaggcg aaatgcatgg gccagaatta ccagatacaa ttttcttgct
    65821 tggacgtgaa aaatcaattc agcatatcga aaacatgcta aaagaaatct ctaaataaag
    65881 aggatacaat aatggacatc tgggaaaaga tgtacgaaga agcacagaaa ctatacaatc
    65941 cacatgaagt atcagacttt gtttatgcca atcatgttgt agctgcagta gaagcaggag
    66001 atggacaaat atttacagga ttctgtatgg agggaacctg tggtgttttc catctctgcg
    66061 cagaacgggc ggccctcttc aatatgtacc aattttcagg acaaactaag gttaagaaag
    66121 tattagcctt tcgagacaaa ccaccttatg gtggaagttc agccatgcct tgcggtgctt
    66181 gtagagaatt ccttttagag cttaatgctg aaaataaaga tgcagaattc atgatggact
    66241 ataatataag aaaaacagtt aaagtcgcag aactaatccc ttattggtgg ggaaaagaac
    66301 gtgcttctaa gtttaatgag cgatagaaga gtcagtataa ttctggcttt ttttactata
    66361 agtt
//
LOCUS       DAVKZB010000003        50398 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKZB010000003 DAVKZB010000000
VERSION     DAVKZB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.003"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            658..789
                     /locus_tag="VXA80_000133"
     CDS             658..789
                     /locus_tag="VXA80_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEV0352698.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKELEE"
     gene            786..980
                     /locus_tag="VXA80_000134"
     CDS             786..980
                     /locus_tag="VXA80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352699.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            967..1851
                     /locus_tag="VXA80_000135"
     CDS             967..1851
                     /locus_tag="VXA80_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352700.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            1853..2590
                     /locus_tag="VXA80_000136"
                     /pseudo
     CDS             1853..2590
                     /locus_tag="VXA80_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2590..3687
                     /locus_tag="VXA80_000137"
     CDS             2590..3687
                     /locus_tag="VXA80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0352701.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            3688..4287
                     /locus_tag="VXA80_000138"
     CDS             3688..4287
                     /locus_tag="VXA80_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0352702.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            4712..5722
                     /gene="ccpA"
                     /locus_tag="VXA80_000139"
     CDS             4712..5722
                     /gene="ccpA"
                     /locus_tag="VXA80_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV0352703.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            5786..6214
                     /locus_tag="VXA80_000140"
     CDS             5786..6214
                     /locus_tag="VXA80_000140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352704.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDETNEVSSKHVFEVVDRTDEVSSKHVFEVVDETDE
                     VSSKHVFEVVDETDEVSSKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(6243..7205)
                     /locus_tag="VXA80_000141"
     CDS             complement(6243..7205)
                     /locus_tag="VXA80_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV0352705.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            7271..8659
                     /locus_tag="VXA80_000142"
     CDS             7271..8659
                     /locus_tag="VXA80_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0352706.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(8694..9146)
                     /locus_tag="VXA80_000143"
     CDS             complement(8694..9146)
                     /locus_tag="VXA80_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV0352707.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            9308..9602
                     /locus_tag="VXA80_000144"
                     /pseudo
     CDS             9308..9602
                     /locus_tag="VXA80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9758..10972
                     /locus_tag="VXA80_000145"
     CDS             9758..10972
                     /locus_tag="VXA80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0352708.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            11117..11251
                     /gene="rpmH"
                     /locus_tag="VXA80_000146"
     CDS             11117..11251
                     /gene="rpmH"
                     /locus_tag="VXA80_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV0352709.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            11535..12434
                     /locus_tag="VXA80_000147"
     CDS             11535..12434
                     /locus_tag="VXA80_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV0352710.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKEKV
                     KVAILQANGLALDGATINVAGYGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATP
                     EYKLETTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESA
                     KNAIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETI
                     GVQAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQNVTYQSPAGKQLPNTGSASSAAL
                     ASLGLVVATSGFALLGRKTRRRK"
     gene            12739..13512
                     /locus_tag="VXA80_000148"
     CDS             12739..13512
                     /locus_tag="VXA80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV0352711.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            13512..14072
                     /gene="rnmV"
                     /locus_tag="VXA80_000149"
     CDS             13512..14072
                     /gene="rnmV"
                     /locus_tag="VXA80_000149"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV0352712.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKSKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            14144..15043
                     /locus_tag="VXA80_000150"
     CDS             14144..15043
                     /locus_tag="VXA80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0352713.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            15312..17342
                     /locus_tag="VXA80_000151"
     CDS             15312..17342
                     /locus_tag="VXA80_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0352714.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTEIGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            17344..17970
                     /locus_tag="VXA80_000152"
     CDS             17344..17970
                     /locus_tag="VXA80_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352715.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKTTLLNILGK
                     LEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQKIS
                     KVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPTAA
                     LDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            17963..18256
                     /locus_tag="VXA80_000153"
     CDS             17963..18256
                     /locus_tag="VXA80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352716.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            18507..19379
                     /gene="rsmA"
                     /locus_tag="VXA80_000154"
     CDS             18507..19379
                     /gene="rsmA"
                     /locus_tag="VXA80_000154"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV0352717.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            19381..20259
                     /gene="rsgA"
                     /locus_tag="VXA80_000155"
     CDS             19381..20259
                     /gene="rsgA"
                     /locus_tag="VXA80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV0352718.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQDFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            20270..20926
                     /locus_tag="VXA80_000156"
     CDS             20270..20926
                     /locus_tag="VXA80_000156"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0352719.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRSHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            20889..21551
                     /locus_tag="VXA80_000157"
     CDS             20889..21551
                     /locus_tag="VXA80_000157"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV0352720.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            21553..22809
                     /locus_tag="VXA80_000158"
     CDS             21553..22809
                     /locus_tag="VXA80_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV0352721.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSSDHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            22799..23740
                     /locus_tag="VXA80_000159"
     CDS             22799..23740
                     /locus_tag="VXA80_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV0352722.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLTFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            24007..24834
                     /gene="purR"
                     /locus_tag="VXA80_000160"
     CDS             24007..24834
                     /gene="purR"
                     /locus_tag="VXA80_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV0352723.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEIGNIFDEDK"
     gene            24901..26151
                     /locus_tag="VXA80_000161"
     CDS             24901..26151
                     /locus_tag="VXA80_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV0352724.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVCKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            26277..27071
                     /gene="pflA"
                     /locus_tag="VXA80_000162"
     CDS             26277..27071
                     /gene="pflA"
                     /locus_tag="VXA80_000162"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV0352725.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(27146..28072)
                     /gene="yidC"
                     /locus_tag="VXA80_000163"
     CDS             complement(27146..28072)
                     /gene="yidC"
                     /locus_tag="VXA80_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0352726.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(28150..28428)
                     /locus_tag="VXA80_000164"
     CDS             complement(28150..28428)
                     /locus_tag="VXA80_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV0352727.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            28462..29202
                     /locus_tag="VXA80_000165"
     CDS             28462..29202
                     /locus_tag="VXA80_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV0352728.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            29258..29941
                     /locus_tag="VXA80_000166"
     CDS             29258..29941
                     /locus_tag="VXA80_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV0352729.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            30053..31045
                     /locus_tag="VXA80_000167"
     CDS             30053..31045
                     /locus_tag="VXA80_000167"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV0352730.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            31110..31649
                     /gene="rsmD"
                     /locus_tag="VXA80_000168"
     CDS             31110..31649
                     /gene="rsmD"
                     /locus_tag="VXA80_000168"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV0352731.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQITKEIGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            31639..32127
                     /gene="coaD"
                     /locus_tag="VXA80_000169"
     CDS             31639..32127
                     /gene="coaD"
                     /locus_tag="VXA80_000169"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV0352732.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            32111..33148
                     /locus_tag="VXA80_000170"
     CDS             32111..33148
                     /locus_tag="VXA80_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV0352733.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            33206..33436
                     /locus_tag="VXA80_000171"
     CDS             33206..33436
                     /locus_tag="VXA80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV0352734.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            33460..34743
                     /gene="murA"
                     /locus_tag="VXA80_000172"
     CDS             33460..34743
                     /gene="murA"
                     /locus_tag="VXA80_000172"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0352735.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            34736..34924
                     /locus_tag="VXA80_000173"
     CDS             34736..34924
                     /locus_tag="VXA80_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0352736.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            34963..35787
                     /gene="endA"
                     /locus_tag="VXA80_000174"
     CDS             34963..35787
                     /gene="endA"
                     /locus_tag="VXA80_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV0352737.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLKWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKVTRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            36016..37347
                     /locus_tag="VXA80_000175"
     CDS             36016..37347
                     /locus_tag="VXA80_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV0352738.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(37545..37615)
                     /locus_tag="VXA80_000176"
     tRNA            complement(37545..37615)
                     /locus_tag="VXA80_000176"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37581..37583),aa:Cys,seq:gca)
     gene            38031..41642
                     /gene="rpoB"
                     /locus_tag="VXA80_000177"
     CDS             38031..41642
                     /gene="rpoB"
                     /locus_tag="VXA80_000177"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0352739.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            41675..45352
                     /gene="rpoC"
                     /locus_tag="VXA80_000178"
     CDS             41675..45352
                     /gene="rpoC"
                     /locus_tag="VXA80_000178"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV0352740.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            45463..45876
                     /gene="ndk"
                     /locus_tag="VXA80_000179"
     CDS             45463..45876
                     /gene="ndk"
                     /locus_tag="VXA80_000179"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV0352741.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(46805..47437)
                     /locus_tag="VXA80_000180"
     CDS             complement(46805..47437)
                     /locus_tag="VXA80_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352742.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(47439..49454)
                     /locus_tag="VXA80_000181"
     CDS             complement(47439..49454)
                     /locus_tag="VXA80_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0352743.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFGALTLISQISQLRSSGIRLISGEKRWSIFLRPVGDDLKGIAVGFSLAGVLTI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNFITSTEWHDY
                     SPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            complement(49557..49853)
                     /locus_tag="VXA80_000182"
     CDS             complement(49557..49853)
                     /locus_tag="VXA80_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0352744.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPYNW
                     GAFSNYYHGSQYHWAYVGSNERDNQKTVYAGAHSAAYAFVNTNLGERITFDAGW"
BASE COUNT        14836 a         9561 c        11442 g        14559 t
ORIGIN      
        1 acagagatat gtgagtgtgt atttttaaaa ctagatatta tatacattaa gagggaatca
       61 gtttggttct ctcttttttg attttcagta attcattttg gcagcgtata ctttttgtct
      121 ccagtctttt caataaatac cgtagtttag gtatacacat tgaaatttaa cagattttat
      181 aactcttata aatctttaag aaagaaatag aagggttttg ttcaacgtat aatttagaga
      241 attgaaataa gatatgaaca gctctatcag gaaagttaaa ttaatttata gaaatatttt
      301 agtatttaag gcctactgtt atagattcaa tacactatat atgtgtttgt ctgataaaaa
      361 tttctactct ttttgatttt aaataagtat tagtttacat tatggtgtga attgggtttg
      421 atatctcttt tgaggaagtt gccttagatt tttctaattg tgttttattg tatagtgtat
      481 cttgcttgtt ttgaacagaa ttttttatga catttgtcat attttctagt gacagaagct
      541 tctgtctcct ctgatttcaa aagactataa ttgtagtatg aaatggagga agaagagatg
      601 agaaataaaa tgattatcgc agtgagttta gtagtagcag gagttatgac ctaactcatg
      661 ttttcgggat tggatgagag tttctatcat tttccttggg agctctttgc tggctttgga
      721 atgatgtctt ggcttgttag agaaggtttg aaattagtcg gagatgtgaa aaaggagctt
      781 gaggaatgaa gagagggatc atctatttct ttatcggcct gtcactcttg gtatggttag
      841 tggaaatgtt tacaggttgg tttgaccaaa ccttgcttcg ccaattcatt cgtggtgctt
      901 tggggtttgg atttatgatt ttcgtcgttt tccttatgag aatggagtgg ttgaaaggag
      961 aatatcatga atatgattaa ggtagaaagc ctaaataaaa acatcaaggg caaggctatt
     1021 ttgaagggta tttcctttga ggtagctgaa ggtgaatgcg tcgccttgat tgggcccaat
     1081 ggtgctggga agaccacact cttggactgt ctgcttggag ataaactggt cacaagcggt
     1141 caagtatcca tccaaggctt gtcagtgacg agttctcagt tagactatat tagaggttat
     1201 ctgcctcaag aaaatgtcat cgttcagaaa ttaaaggtca aagagttgat tgctttcttt
     1261 caacgtatct atccaaattc cttgagcgat caggaaatcg atcaactatt gcagtttgac
     1321 cagcaacaaa aagagcaatt cgcagaaaaa ttgtcaggcg ggcaaaagcg tctcttctct
     1381 tttgtcttga ccttgattgg gcgaccaaag cttgtctttt tagatgaacc aactgctgcc
     1441 atggatactt caactcgtca acgcttttgg gaaatcgttc gggacctaaa agcgcaagga
     1501 gtcacgattc tctattcgtc tcattatatt gaagaggtag agcatacggc tgaccggatt
     1561 ttggttttaa ataagggaga gttgattcgt gatacgacgc ctctagctat gcgtagtgag
     1621 ggaattgaaa agcattttat ccttcctctg gcatacaagg aagtcattga gcagtctaac
     1681 ttggttgaaa actggtcaca aaaacaggat gctctacaag tagtcacacg cgaagcagat
     1741 gctttttggg aactgttagt tcaagcagga tgtggcatac aagaaattga agttaataat
     1801 cgtagtttgt tggatacaat ctttgaagaa acgcagaagg gagataacta agatgaaacg
     1861 atggctagca cttagtaaga tagaattttt attgaccaaa tgacaattga tctattatct
     1921 attgtcagta gggatgccga cagccttcta tctattcttt tctggcatgt accaggacac
     1981 accaggtgga cccgcgaatt ttatgcgtga ttacctcatc tccatgactg gcttttccat
     2041 gatgtcgacc gctatctttt cattcccagc tgtcttacat accgacaaga tgaacaactg
     2101 gcagaaaata ttacgtcata gcccagtaaa tatggtagaa tattatctat caaagataac
     2161 aagtatgctg gttgattatt tggtctcaat tctggttgtt tttacagttg gacattttgt
     2221 aagaggtgta gacatgcctc tagtaaaatg ggttggagct gcgattttgc tgattttggg
     2281 aagtattgct tttgtagcac ttggcttaac cttgacgctg ttaccaacta gtcagctgat
     2341 gtctgtcgtg ggcaatcttc tttatctagg attggctgtt ttaggcggac tctggatgcc
     2401 cgtctcttta tttccagact ggatgcaagc aatcgggaag cgtttaccaa gttatcagtt
     2461 gatggaatta atcaagacct tcttaaatga gggtggaatc aatctatcag ccacagttta
     2521 tctacttgtt ttttcagcag ttttatttgg tttgactatt taccttcaag gtcataagga
     2581 gaatgcttaa tgcttgaaag actgaaaaga atacattata tgttttggat cagtttaatt
     2641 tttatgattt tccccatcct gcctgtagtg actgggtggc tttctgcctg gcatttattg
     2701 attgatattc tatttgtagt ggcatatttg ggtgttttaa caactaagag ccagcgccta
     2761 tcttggctat attggggcct catgctgact tatgtagttg ggaatactgc ctttgttgct
     2821 gttaattata tctggttttt ctttttccta tccaatctct taagttatca tttcagcgta
     2881 cgtagtttaa agtctttaca tgtctggact tttcttcttg ctcaagtcct tgttgtgggg
     2941 caactgttga tttttcagag aatcgaagtt gagtttctat tctatctact tgtaattctt
     3001 acttttgtcg atttaatgac ttttggattg gttcggattc gtattgtcga ggatttgaaa
     3061 gaagctcagg tcaagcaaaa tgcccagata aatctattgc ttgctgaaaa tgaacgtagt
     3121 cgtatcggtc aggatttgca tgatagtctg ggacatacct ttgctatgct gagtgtcaag
     3181 acagatttag ccttgcagtt atttcagatg gaggcttatc cacaggtgga aaaggaatta
     3241 aaagaaattc accagatcag caaggattcc atgaatgaag tgcgaaccat tgtggaaaat
     3301 cttaagtcta gaactttgac atccgaacta gagactgtga aaaagatgtt agaaattgct
     3361 ggaattgagg ttcaagttga gaatcacttg gacaagtcta gcttaactca ggaattggag
     3421 tcaacggctt ccatgatttt gcttgaatta gtgaccaata tcatcaaaca tgctaaagcg
     3481 tctaaagtct acttgaaatt agaacggaca gagaaagaac tcattctaac agtgagagat
     3541 gatggctgtg gctttgcctc tataagcggg gatgagctcc atacagtccg aaatcgtgtt
     3601 tttccatttt caggagaagt aagtgtaatc agtcagaaac atccaactga agtgcaggtt
     3661 cgactacttt ataaggagag aaagtagatg aaagtattag tcgcagaaga tcaaagtatg
     3721 ttgcgagatg ccatgtgcca gttgctcatg cttcaaccgg atgtagagtc tgtctttcaa
     3781 gccaagaatg ggcaagaagc aatccaacta ttagaaaagg agtctgtaga tatcgccatc
     3841 cttgacgtag aaatgcctgt taagacaggt cttgaagtct tggagtggat acgagcagaa
     3901 aagcttgaaa caaaggtggt tgtggtgacg accttcaagc gtcctgggta ttttgaacgt
     3961 gcggtcaagg ctggagtaga tgcttatgta ttaaaagaaa gaaacattgc agacctcatg
     4021 caaaccttgc acaccgtcct cgaaggacgc aaggagtatt cgcctgaatt gatggaagtg
     4081 gtgatgatgc atcccaatcc attaacggag caagaaatcg cagttttaaa gggaattgct
     4141 cagggcttct ctaaccaaga aattgcagac aaactttatc tatccaacgg aacagtccga
     4201 aactatgtca ccaatattct ttcgaaacta gatgcaggta atcgaacaga ggcagctaat
     4261 atcgcgaaag aatctggttg gttatgatga tataatattt tcatgaagac tatgtgctaa
     4321 aattgacggt agtgaatgga acaataccta tagtagattg aaactagaat agtacacctc
     4381 tacttctaaa acattgttag aaatcgattt gactgtcctg atcgatttat cctgttctta
     4441 tttcatttta ctataacttt aggagggctt agtttcctcc tttttgttta gtctaaggtg
     4501 gtagcaagga gctaaaccta ggttttctat gaaatttttt caatcaatct tcaaaaaaca
     4561 cttgaaagtg tttacaatta gtgatagaat ggaataagta gaaatatgaa aacgaaattt
     4621 tcacatcttc acttttgtaa gtagatatag agcttcaatc agtacctcta cttgtaaaag
     4681 aatgtgtgag atagaaagga caggaaaagg aatgaatgca gatgatacag taaccattta
     4741 tgatgtcgct cgtgaagcag gtgtttccat ggcgacggtc agccgtgtgg tcaatggcaa
     4801 taaaaatgta aaagagaata cccgtaaaaa agtgctagag gtaattgatc gtttggatta
     4861 tcgtccaaat gcagttgcgc gtggtcttgc aagtaaaaag acaaccactg tcggtgtcgt
     4921 gattccaaat attaccaatg gttatttttc gagtttggct aaggggattg atgatattgc
     4981 agaaatgtac aagtacaata ttgtcctagc taatagcgat gaagataacg agaaagaagt
     5041 ttctgttgtc aataccctct tttcaaagca ggtagatggc attatctata tggggtatca
     5101 cttgacagat aaaattcgct cagaattttc gcgttcacgt actccgattg ttctcgcagg
     5161 aactgtcgat gttgagcacc agttgccaag tgtcaatatt gactataagc aagcaacaat
     5221 tgatgcagtg agttaccttg ctaaagaaaa tgagcgtatt gctttcgtta gcggtccgct
     5281 agtggatgac atcaatggta aggttcgttt agttggctac aaggaaacct tgaaaaaagc
     5341 aggaatcact tatagtgagg gtttggtatt tgaatctaaa tatagctatg atgatggtta
     5401 cgccttagca gagcgtttga tttcatcaaa tgcaactgca gcagttgtga caggtgatga
     5461 gttggcagca ggagtcttga acggtttggc tgataagggt gtttctgtgc cagaagattt
     5521 tgaaattatt actagtgatg attcacaaat ctcacgcttt acccgtccaa acttgacaac
     5581 gattgcccaa cctctttatg accttggtgc cattagtatg cgtatgttga ccaagattat
     5641 gcataaggaa gagttggaag aacgtgaagt tctcttacct catggtttga cagaacgtag
     5701 ctcaacacga aaacgtaaat agaaaaaatc agggaatcga gaagatttcc tgattttgtg
     5761 ctcttaaaca agagatgttg acttaatgaa taccatagag cggactaggc ggctggttaa
     5821 aggctgcgca acacactgtt ttgaggttgt agatagaact gacgaagtca gctcaaagca
     5881 ctgctttgag gttgcagata gaactgacga agtcagctca aaacatgttt ttgaggttgt
     5941 agatgaaact aacgaagtca gctcaaaaca tgtttttgag gttgtagatg aaactaacga
     6001 agtcagctca aaacatgttt ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     6061 tgtttttgag gttgtagatg aaactgacga agtcagctca aaacatgttt ttgaggttgt
     6121 agatgaaact gacgaagtca gctcaaaaca tgtttttgag gttgtagatg aaactgacga
     6181 agtcagtaac catacatacg gtaaggcgac gtgacgtggt ttgcagagat tttcgaagag
     6241 tattagcctt ccatatagtc tttcaaagcc tgtcctgtta gtccggcatt gagggcgatg
     6301 aggagtttca agcgagcttt ttgggcgttg agttctttaa caaagaaaac gcctgctttt
     6361 tgcaactgta cgcccccacc ctggtaggca taaacaggct cggcaatacc gttaaagcat
     6421 cgtgatacca gagcgactgg aattcctttt tgcagaaggc tttctaattt ttgagccgtt
     6481 tctttgggaa tattaccagc tccgaaggct tggataatca aaccgtccaa gtgttctaaa
     6541 tccagcatat caatcagctc atctgtcata ccagcataag ccgagatgat agggactaaa
     6601 ccttgtatgt gatcaaggtc aaagcgaaca cgaggttcag ctgttttgaa gtagaggatt
     6661 tcctgtttca tgatgagacc aagggggcca tgtgttggag tctggaaggt gctgacatta
     6721 gtcgtatgtg ttttggtgac atacttggca gcgtggattt catcgttcat aacgaccaaa
     6781 actcctttgt cagcagccct gtcatcgctg gccactcgta aagcacttag gtaattataa
     6841 acaccatcac taccgagttc attggagcta cgcatggctc ctgttagaac gataggcata
     6901 tggggaactt ccatggtatc aaggaaatag gctgtttcct ctaaagtatc ggttccgtgt
     6961 gtgatcacca ctccatcgta gttatctgct tcctctttaa ttttctggta gaggaccagc
     7021 atatgtttgg gtttgatatg gggacttgga aggttaaaaa agtccaaggc gtggacttgg
     7081 attccttcaa gtgggttgga cacatggttc atgggattat ctgaactcgt cacaacagcg
     7141 ccagaagcat cggcctgcat ggaaatagtt ccacccgtat gtaaaacaag gattttctta
     7201 ggcatgattt actcactctt tctgaaatgt ggtataatca ttgtatcaca aaaggggaga
     7261 ttgggatagg atggaagtca aagctgtttt ttttgatatc gatggaacct tggtcaacaa
     7321 tcgcaagagt gttttgaaat ccactaagga tgcgattaag attgtcaaag aacaaggggt
     7381 actagttggc gtagcgacag ggcgaggacc tttttttgtt aaggaattga tggacgattt
     7441 ggatctggat tttgcggtaa cctataatgg ccagtatatc tttagtaaag acagagtctt
     7501 gttcacgagc cctatttcca agttacattt gcgccatcta attagctatg ctaaaaaaga
     7561 gggcacggag attgccttag ggaccaagga tgccatgttg ggttctaaaa tcatgtcctt
     7621 tggtctgggt tctttttccc aacgaattag tcgcttcgtt ccctctgttt taactcggac
     7681 ggtgagtcag tcttttaatc gcatggtcag caaggttgtt ccccaaaagg aagaggacct
     7741 gcttcacctg atgaatcaac ctatttacca agttttgatg ctgatgacac cagaagaatc
     7801 tgagaaggcg gcagctgatt ttgaagactt gaaattgaca cgtagcaatc cttttgcatc
     7861 ggatgtcatc aatcagggaa attctaaatt agaaggcatt cgccgagttg ggaaagaata
     7921 tggctttgac ctcaaccaag tcatggcttt tggtgactca gataatgacc ttgaaatgct
     7981 ggctggcgtc ggtatgtcgg tcgctatggg aaatggtagt agcagtgtca aggaagctgc
     8041 caagcacatt accactagca atcaacaaga tgggatccac aaggccttgg aacattttgg
     8101 tgttttgtct tcagaaaaag tttttgtcag ccgtgactat catttcaata aggtcaagac
     8161 cttccaccac atgatggatg aacgaaccca agaagaacct cgagcttggg atttagaggg
     8221 tgcaacccat agggctggtt ttaaaataga agaattggtg gagtttgttc gatcagccag
     8281 tccttctgaa gaagattttg gtcaagctgt atcgcaactt catcaggccc ttgataaggc
     8341 agcaggtaaa gtagccaaga agacacctgc tcagcaagat ttgatagggc aagtggatgc
     8401 cttgattgac acgctttact tcacctacgg cagttttgtc ttgatggggg tggacccaga
     8461 acgtattttt gatattgtcc atcaggccaa tatggggaaa atttttccag atggcaaggc
     8521 tcattttgat ccagtgaccc ataaaatctt aaaaccagat aattgggaag aaaaatatgc
     8581 tccagaacct gctattaaaa aggaactgca gcgccagctc aaggcttacg aacgccataa
     8641 agagagaaat aaatcgtaaa caaaaaggag ctaggggctc cttttctttg tgattataag
     8701 gttttttctt gttctctcac aaccagcaaa tcgaccttag catggcggag tatgtattca
     8761 gatgaagagc cgaccaagag gcgttcaaag gcgttgagac cagttgcgcc aacgaggatg
     8821 aggtccactt cctcggcatc tggaatagta cgtgctagca gggtctttgg atttcccatt
     8881 tcaatgacga tatgaacatc tgctacccca gcatctttag cacgtttttc gtactctttc
     8941 atcagacttt cagcgtcgac ttggagttct tcgtaaactt cagcatcaaa ggtggatacg
     9001 ctttggagag cgcgtgtgtc aatgacatgt gcgatggtga gtttagcgtc gtttcgtaga
     9061 gcagaatgaa ctcccttgac aaaagccaag tccgcttcct tagaaccatc gattgcgacc
     9121 atgatatttt ggtaacgttg tgccataatg aaacctcctg aaattttctt actataattg
     9181 taaacctttt cactatagaa gtaaagcaaa aaaagtattt ttcaaaagaa tattagggct
     9241 taaactctat ataaatatat gataaaataa aagaaaggat agcaggaaag aagtgaagga
     9301 agggagaatg atgaaaaact cttttcaaaa atcaaatttc ctttattatg gaattatcct
     9361 agtttcagtc ttggtagaag tgatcatgat ctgtcaatta gagagtctgg taccgcttct
     9421 ttatccaagt tttattggat ttttagtttt tcatgtactc taccatctaa tcttatttct
     9481 agttgcaaaa cgaagtgggc gttgggatta cttgatgata tgggggcttt tcctcatgtt
     9541 taatcttctt tatgactcct ttctaggctt gcttttttct aggtttatct tttggtatgt
     9601 gatgtgtctt tgcaaaatac ctgctatctc tacgttctta acctcgcctt tagcaaggtt
     9661 tttcttctat acgaaactag taacacattt cagtaaactt gtcgcattct ctttctagtg
     9721 gtataatgtt actatcctga tgaattgagg aaacaagatg aaagaatata acaagtctag
     9781 taagttagag catgttgctt atgatatccg tggtcccgta ttggaagaag ccatgcggat
     9841 gcgagcgaat ggagaaaaga ttttacgtct gaatacagga aatccagcag aatttggctt
     9901 tacagcgcca gacgaggtca ttcatgactt gattatgaat gcgcgtgata gtgaaggcta
     9961 ttctgactcc aaagggattt tctcagcccg taaggccatc atgcagtatt gccagctgaa
    10021 gaaattccca aacgtagata ttgatgatat ttaccttgga aatggtgtca gtgagttgat
    10081 tgtcatgtcc atgcaggggc ttttggacaa tggggatgag gtcttagtgc caatgccaga
    10141 ctatcctcta tggacggcgg ctgtcagcct agctggagga aatgccgttc actatatctg
    10201 tgatgaagct gtggaatggt acccagatat tgacgatatc aagtcaaaaa tcacttccaa
    10261 taccaaggca attgtcctta tcaatccaaa taacccaact ggagcccttt atcctaagga
    10321 actcttgctg gagattattg agattgcccg tcaaaacgat ttgattatct ttgcggatga
    10381 aatctatgac cgcatggtaa tggacggaca tgtgcatacg cctgtggcga gcttggcacc
    10441 agatgtcttc tgtgtcagca tgaatggtct gtcaaaatcc caccgcatag caggtttccg
    10501 tgtgggttgg atggtcttgt ctggccctaa gactcatgtt aagggctata tcgaagggct
    10561 caatatgctg tccaatatgc gcctttgctc taacgttttg gcccaacaag tcgtacaaac
    10621 ttccttgggg ggtcaccaat cagtcgatga attgcttctt cctggtggac gaatctacga
    10681 gcaaagaaat ttcatctata atgccattca agatattcca ggtttgtctg ccgttaaacc
    10741 caaggcgggg ctctatatct tcccaaaaat cgaccgcaat atgtaccgta tcgatgatga
    10801 tgagcagttt gtccttgatt tcttgaagca ggaaaaggtt ctcttggttc atggtcgagg
    10861 ctttaactgg caggaaccag accacttccg tatcgtttac cttcctcgtg ttgatgagtt
    10921 agcccaaatc caagaaaaga tgactcgttt cttgaaacag tatcgtagat agggcttgca
    10981 ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga atcagataga
    11041 atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat acaaaatcta
    11101 tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc gtgcgcgcaa
    11161 acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg cagctcgtcg
    11221 tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa aaatcagtag
    11281 gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca aatggattcg
    11341 gaagcgatgt tacagtagat tgaaactaga atagtacacc tctgtttcta aaacattgtt
    11401 agaaatcgat ttgactgtcc tgatcgattt gtactgttat tattttattt tactataaag
    11461 ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca gctattacaa
    11521 ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact aaactgactg
    11581 ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat ataactcatt
    11641 taacagatga agaaaaagaa aaagttaagg ttgctatttt acaagcaaat ggtttagcat
    11701 tagacggagc gacaatcaat gtagctggat atggtacagc aacaatcaca ttcccagatg
    11761 gttcagtagt gacgattcta ggaaaagata cagttcaaca atctgcgaaa ggtgaatctg
    11821 taactcaaga agctacacca gagtataagc tagaaactac accaggtgga gataagggag
    11881 gcaatactgg aagctcagat gctaatgcga atgaaggcgg tggtagccag gcgggtggat
    11941 cagctcacac aggttcacaa aactcagctc aatcacaagc ttctaagcaa ttagctactg
    12001 aaaaagaatc agctaaaaat gccattgaaa aagcagccaa gaacaagcag gatgaaatca
    12061 aaggcgcacc gctttctgat aaagaaaaag cagaactttt agcaagagtg gaagcagaaa
    12121 aacaagcagc tctcaaagag attgaaaatg cgaaaactat ggaagatgtg aaggaagcag
    12181 aaacgattgg agtgcaagcc attgccatgg ttacagttcc taagagacta gtggctccta
    12241 atgctgctcc taagacaaca agtgcaccgc aagcaactgc aggaacaatg caaaatgtta
    12301 cctaccagtc acctgctggc aaacaattac ctaacacagg ttcagcatca agtgcagcac
    12361 ttgctagtct tggtctagtg gtggcaacaa gtggttttgc tttgctagga agaaagacta
    12421 gacgtagaaa atagaacagc tagaaaattc tattctctac ttaaagttag attataaggg
    12481 ggattttgag aagtcatcaa tcctagtgat gggtgagaaa agtgagaacc caagataatc
    12541 acatacttta gctgaatagg aatattctat caatgtagcc aatctcttct gtctctaact
    12601 gtggaatagg agatgggcaa tatcggatag aaaagatagc agaatagctc tctattgaag
    12661 agaggagggg aaaccgaaaa attaggtgcc cctcctcttt tttggtataa tagaagatag
    12721 aaaacgaggt tagaagagat gatttttgat acacatacac acttgaatgt agaagaattt
    12781 gcaggtcgtg aggcagaaga aattgccttg gctgatgaga tgggtgtgac acagatgaat
    12841 attgttggtt ttgataaacc gacgattgag catgccttgg agttggtaga tgagtatgag
    12901 cagctctatg cgactattgg ttggcatcct acagaagctg gtacttatac agaggaagtt
    12961 gaggcttact tgttggataa gttaaaacat tccaaggttg tggctttagg tgaaattggc
    13021 ttagactacc attggatgac agcgcccaaa gaggtgcagg agcaggtttt tcgccgtcag
    13081 attcagctat ctaaggactt ggatttgcct tttgttgtcc atacccgtga tgcgctggaa
    13141 gatacttatg agattatcaa gagtgagggc gttggtcctc gtggtggtat catgcattca
    13201 ttttcaggga cgcttgagtg ggcagagaag tttgtggatc ttggtatgac catttccttc
    13261 tcaggagtgg tgactttcaa gaaggcaact gacctccaag aagcagctaa agagttacct
    13321 ttggacaaga ttttggtgga aacagatgcg ccttacttag cacctgtacc caagcgtggt
    13381 cgtgaaaata aaacagccta tactcgctat gtggtcgact ttatcgctga cttgcgtggt
    13441 atgacgacag aagagctggc ggtagcaacg actgcaaatg cagaacgaat ttttggattg
    13501 gacagcaagt aatgaaagag aaaatttctc aagttatcgt ggttgaaggt cgcgatgata
    13561 cggtcaatct caagcgttat ttcgatgtgg agacctatga gacacgaggt tctgccatca
    13621 atgatcagga tatagagcga attcagcgtc tacaccaacg tcatggagtc attgtcttta
    13681 cagacccaga ttttaatggg gagcggattc ggcgcatgat tatgacggcc attccaacag
    13741 ttcagcatgc ctttctcaag cgagatgaag ctgttcccaa gtccaagtcc aaggggcgtt
    13801 ctctgggaat tgagcatgcc agctatgaag acctgaaaac ggctctagct caggtgacag
    13861 aacaatttga acatgagagt cagtttgaca ttagtcgtag cgatttgatt cgccttggtt
    13921 ttctagcagg ggcagacagc cgtaagcgta gagaatatct cggagagact ctccgaatcg
    13981 gctattccaa cggcaagcaa ctcctcaaac gcctagagtt gtttggggtt actttggcag
    14041 aagtggaaga agctatgaaa tcttatgagt aggaaagatg tagccgttac aattttttaa
    14101 gtttcacagt atttttcgaa gcaggtagaa gaggaggcgt ctgatattaa ttggtcaaaa
    14161 aattaaagag attcggatag aaaaaggaat tagtcgtcca gatttttgtg gagatgagca
    14221 agaactgaca gttcgtcaac tgtcgcgaat tgaaagtgga gcttcgcaac cgagcttgcc
    14281 caagttagcc tatattgctc gccggctagg agttccagtt tatagcctta tgccggattt
    14341 ttcagctctt cctcctgctt atttagaatt gaaataccag attttacgtg aaccaatcta
    14401 tggtaaagaa gaggagtacg ataagaagga agcgtgtttg gaagagattt ataaaacata
    14461 ctttgataat cttcctaaag aagaacaatt agcatgtgaa gtattgcagg cgtgtttgga
    14521 tacttctaga actagaaggc ctgaatatgc agagttaata cttgaggaac atatgtctca
    14581 gatcatagaa aaagaagttt attcaacaaa tgatatgttg ttgattcgtt tgttttttta
    14641 tcaaatgctc attagaaaag atcttgccaa atttataaat caaatcgaaa agctaatgct
    14701 ctttcttttg gaacagaaaa aggtaactaa attagaaaac ttctttataa ttagagatac
    14761 tcttatttca ggaatgtgtt gtcttgaaaa ggtaggagta actgattgtt ttaatgatta
    14821 tctatcgtgt ttacaagaaa ttatggataa aactcaagat tatcaaaaga aacctcttgt
    14881 atttatgttt ttgtggaagc aagcattaag agaagaaaga gattttagtt tagctgaatc
    14941 attttatcag tcttctaaaa catttgcgca gctaattgga gatgaatttc tagtaaagaa
    15001 attgacagag gaatggcaag aggatgtcaa aaaatattta taaacatagt gaatcagtga
    15061 caaaaatgtc cttgtcctcg tatcaaaaca gttctaaagt tcgtctttag aactgttttt
    15121 ttagatataa gctaaaaata atatgaaata gttagatttt aaggacattg atgtccttga
    15181 tagttcttga aaaaacggat ataatgtatt tgaagatgtt gctagcagaa agaacttaat
    15241 aagcttctta acatcgcagt gactgtggtc accttctcac taagtgacca agtaactttt
    15301 tggaggaaat gatgaaacgt ttatttattt tgatttcaat ggtattggta tcgctttata
    15361 tggtgataac ttccgttgac catcgagaag agattttatt tggtaactat ccttctgttg
    15421 atgtgacagg aatgatgata aatcaaccag tagctagtcg cgaagaggtg acagaggctt
    15481 tgagtcactt ggcggtagag cacaatagtc tcattgctcg tcgaatcgtt gagccaaatg
    15541 aagctggaga aacacgcttt acctatgcca cttatggtga gggaaagctt ccagaaggtc
    15601 tgaccatttc ctccaaggag agtgcagaaa cgagtgattt attagggtct tacttgattg
    15661 tatcaggaag tttggatgga gtgagcttac agaccacctt gaaagagctt ggttatcaag
    15721 gctttgtttc gaatggagaa gatccatttt cgatagtctt actattgacg gccaccccta
    15781 tggtgctact gagtttagct atttttctgc tgacctttat gagtctgacc ctgatttatc
    15841 ggatcaaatc ccttcgtcag gcagggattc gcttaatagc tggtgagagc ttgtttggag
    15901 ttgctctcag accagtgtta gaagatgtga gacagcttat ctgctcagtg ctggtatcca
    15961 gtcttttggg attggggatt ctctggtatc aaggtgcctt gtttatggca acggtgcaac
    16021 tggtcatcat tgctcttcta ctttatggat tgaccctggc agggatttct accttactaa
    16081 gtgtcgtcta tctacttggt ttacaggaaa atagtctggt ggatctattg aaagggaaac
    16141 tccctctcaa acgtatgatg acattgatga tggtggggca actcttagct gtattggtgg
    16201 tcggatcgag tgcgacagct ctcctacccc actaccgtga aatgcaggaa atggagagag
    16261 ctagcaataa atggagccag tcctcagacc gttaccgtct atcctttggt tggtctagtg
    16321 catttgccga tgaagaagga acgcgtaagg ataatcgtga gtggcagaca tttactgaag
    16381 aacggttagc caatacagac tctttttata ttatgagcaa tgttgacaat ttctcagatg
    16441 gagcagaagt ggacctagat ggcaatcgtc tcagtgacta cacaccgtca gggaatgtta
    16501 tctatgtctc accacgctat ctgatagaag aaaagattac cgtttcttca gagtttatgg
    16561 acaagatgca aaacttgtct gagggagagt ttgggctgat cttgcctgag agtttgcgag
    16621 agcagtctgt ctactaccaa ggattgttta cagattacct gcaaaacttt tcatctgaaa
    16681 gtgtagaagt gacgagtcag aaacactacc tcccacaggt aaggctagct tttacagaaa
    16741 taggacagga acgtttcctc tataatgatg ggtacaagac aacacgccag tacctaaaag
    16801 atccgattat tgtagttcta acgccgcaag cgactggaac aagacctgtt gcagggatgt
    16861 tgtggggaac tacggctaat agtgccttga aactagatcg atatggagac agcatcacag
    16921 ctctaaaaga gcaaggtctg tatcacaagg tttcttactt ggtaaaaagc cagctatttt
    16981 ttgccaaggt actaaatgac aaacgggtgg agttttactc tctccttatt gggacgattt
    17041 tgaccctatc tacggctatc ttgttatttg attccatgaa tcttctctat tttgagcagt
    17101 tcagacggga gcttatgatt aaacgtcttg ctggtatgac aatctatgag cttcatggca
    17161 agtatttact ggcgcaagga ggagttctct tgcttggcct agtcctatct agtattttga
    17221 caagagatgg tttgattagc gctctagttg tagctttgtt tacgcttaac gccctcttga
    17281 ttttagtaag gcaggacaaa aaagaagaag ctggtagcat ggcagtattg aaaggaaaat
    17341 aagatgattg atattcaagg attggaaaag aaatttaatg accgcgcgat tttctctggt
    17401 ttgaatctca agctggagaa gggcaaggtt tatgccttaa tcggaaagac gacgctgctg
    17461 aatatcttgg gaaagctaga aaagatagat ggaggaaggg ttctctatca ggggaaagat
    17521 ttaaaaacca ttcccactcg tgagtatttt cgagatcaga tgggctatct ctttcaaaat
    17581 tttggcctct tagaaaacca atcaatcaaa gaaaatttgg atttgggttt tgttggtcag
    17641 aaaatctcaa aagtagaacg tttggaaagg caagtggggg ctttagaaaa agttaatcta
    17701 gggtatttgg atttagaaca aaaaatttat actttatctg ggggagaggc ccaacgagtt
    17761 gcccttgcta agactatttt gaaaaatcca cccttgattt tggcagatga accaacagca
    17821 gctcttgatc ctgaaaattc agaggaggtt atgaatctct tggtggattt gaaagatgaa
    17881 aatcgaatta tcatcattgc gacccataat cccctagtct ggaataaggc tgatgaaatc
    17941 attgatatga ggaaacttgc tcatgtgtga aaaaatccgt attcgcaggg tatctgatta
    18001 tcctagtgcc agaggtggtt tagaagatat cctcatcatg gaaaatatga ccaatcatct
    18061 ccttttggtt caaatccgag tgcatggcta tttgcttgat tttgctagta ttgaagggca
    18121 aaggcaaaag cattatcgtt tgaaaaattt acctcagacg gttgaactga cagtggatga
    18181 tgtggaggag gatgtggatt tgaccctacc cgaaaatcga agttatcaag aagctgattt
    18241 ttttgatact caatgaaaat caaagagcaa actaggaagc tagccgcagg ctgtacttga
    18301 gtacggcaag gtgaagctga cgtggtttga agagattttc gaagagtatg aacgtatgtt
    18361 tcgagagaag cgctaagtcc ctctttaaag atttccaaga ctatctttct tcatgaggaa
    18421 agatagtttt ttggtatgat tttcattccc aaaatacaag gggaatgtgt tacaatagta
    18481 gtaacagata atagaaaaga gaatagatga gaattgcaga ttatagcgtg accaaggcag
    18541 tgctggagcg tcacggtttt acctttaaaa agtcctttgg gcaaaatttt ttgacggata
    18601 ccaatatcct tcaaaaaatt gtggatacgg ctgaaattga tgatcaggtc aatgtcatcg
    18661 aaatcgggcc aggtattggt gccttgacag aatttttggc tgagcgtgca gcccaagtca
    18721 tggcttttga gattgaccac cgtttggtgc caattttggc agataccctg cgtgattttg
    18781 ataatgtgac tgtagttaac gaagatattc tcaaggttga tttggcgcaa catatccaga
    18841 attttaaaaa tcctaacctg ccaatcaagg tagtggctaa tttgccttac tacatcacga
    18901 cgcctattct catgcacttg attgagagtg ggattccttt tagtgagttt gtggtcatga
    18961 tgcagaaaga agtagcggac cgcatttcag cccagcctaa caccaaggct tacggtagct
    19021 tgtctatcgc cgtgcagtat tacatgacag ccaaggttgc ctttatcgtg cctcgtacgg
    19081 tctttgtgcc agcgccaaat gtggattcag ccatcttgaa aatggtgcgc cgtccagagc
    19141 cagctgtagc agtggaagat gagaacttct tctttaaggt ttccaaggct agttttaccc
    19201 atcgccgtaa gaccttgtgg aataacttga caggttactt tggtaagact gaagaggtca
    19261 aggacaagct gaccaaggct ttggaccagg caggcttgtc accaagtgtg cgtggggaag
    19321 ctctcagctt ggcagaattt gccggtctag cagacgcact taaagggcaa ggactctaag
    19381 atgcagggac aaatcattaa agccttggca ggtttctact atgtggagag tgatggccag
    19441 gtttatcaaa cacgcgcgcg tgggaatttc cgtaaaaaag gccatacccc ttatgttggg
    19501 gactgggtgg atttctctgc cgaggaaaat tcagaaggct atatcctaaa aattcacgaa
    19561 cggaaaaaca gtctggttcg tccgcctatt gtcaatatcg atcaagctgt agtaatcatg
    19621 tccgtcaagg aacctgattt taacagcaat ttgctggatc gtttcttggt tcttttggag
    19681 cacaagggca tccatcccat tgtctatatt tccaaaatgg atttgttgga agatagggga
    19741 gaactggatt tttaccagca gacctatggt gacatcggct atgactttgt gaccagtaaa
    19801 gaggaactcc tgtctttgtt aacaggcaag gttacggtct ttatggggca gacaggtgtt
    19861 gggaagtcaa ctcttctcaa taaaatcgca ccagacctca atcttgaaac gggagaaatt
    19921 tcagacagtc taggtcgcgg tcgccatacc actcgagctg ttagttttta caatctcaac
    19981 gggggtaaaa tcgcagatac accaggattt tcatccttgg actatgaagt atcaagggct
    20041 gaagacctca atcaggattt cccagagatt gctactgtta gccgagattg taagttccgt
    20101 acttgtaccc atacccatga gccgtcttgt gccgtcaaac cagctgttga agagggtgtt
    20161 attgcaacct tccgttttga caattacctg caattcctta gtgaaattga aaatcgtaga
    20221 gaaacctata aaaaagtcag caaaaaaatt ccaaaataag gagaaaccta tgtctcaata
    20281 caagattgct ccgtcaattc tggcagcaga ttatgccaac tttgaacgtg aaatcaaacg
    20341 tctagaagca actggggcag aatatgccca tatcgatatc atggacagtc attttgtacc
    20401 gcaaatcagt tttggtgcag gtgtggtcga gagccttcgt tctcatagta agatggtttt
    20461 cgattgccac ttgatggtgt caaaccctga gcatcatctg gaagattttg cgcgtgcagg
    20521 tgcagacatc atcagtatcc atgtagaagc aacgcctcat attcatggcg ccctccaaaa
    20581 aattcgttca ctcggagtta agccttcagt cgttatcaat cctggcacat cagttgaagc
    20641 catcaagcac gtccttcatc tagttgacca agttttagtc atgacggtta atccaggttt
    20701 tggtgggcaa gcctttctgc cagaaaccat ggataaggtt cgtgagttgg ttgctcttcg
    20761 tgaggaaaaa ggtttgaact ttgaaatcga agtggatggt gggattgatg accaaactat
    20821 tgcccaagct aaagaagctg gtgcgaccgt ttttgtagca ggttcctatg tctttaaggg
    20881 agatgtcaat gagcgagtac aaactctcag aaaacaactg gactagggtt gcagtttttg
    20941 caggcggaaa ccgcggtcat tatcggacag attttgatgc ttttgttggg gtggatcgag
    21001 gctcgctctg ggtcttggaa gaagacttac ctcttgctct agcagtcgga gattttgatt
    21061 ctgtgacgga agaagagcga caggtgattc aaaaacgtgc ccagtatttt gtccaagccc
    21121 gaccagaaaa agatgatacc gatttggaat tggctctctt aaccatcttt gagaaaaatc
    21181 ctcaggctca ggttactatt ttcggtgcct tgggtggccg tattgaccat atgttggcca
    21241 atgtctttct gcctagcaat cctaagttgg caccctatat gcatcaaata gaaattgagg
    21301 atgggcaaaa cttgattact tattgtccag aagggaccag tcagctagaa cctcgttcag
    21361 actacgacta tctagccttt atgccagttc gggatagcca gctgactatt cttggagcca
    21421 agtatgagtt gacagaggaa aattttttct ttaaaaaagt gtacgcttct aacgaatata
    21481 tagataggga agtgtcggta acttgcccag atggttatgt ggtcgtactg catagcaagg
    21541 ataggaggta ggatggaaag tttacttatt ctattattaa ttgccaacct agctggtctc
    21601 tttctgattt ggcaaaggca ggataggcag gagaaacact taagtaagag cttggaggat
    21661 caggcagatc atttgtcaga ccagttggat taccgctttg accaagccag acaagccagc
    21721 cagttagacc aaaaagattt ggaagtggct gtcagcgacc gtttgcaaga agtgcggatt
    21781 gaattgcacc aaggcctgac ccaagtccgt caagaaatga cagataatct cctccaaact
    21841 agagataaga cagaccaacg tctccaagcc ttgcaggaat caaatgagca acgtttggaa
    21901 caaatgcgcc agacggtcga ggaaaaacta gaaaagacct tgcagacacg cttacaggct
    21961 tcctttgaga cagtttctaa acaactggag tctgtcaatc gtggtcttgg agaaatgcag
    22021 acagttgccc gtgatgtcgg agctcttaac aaggttctct ctggaaccaa gacgcgaggg
    22081 attctgggag aattgcaact ggggcaaatt attgaagaca tcatgacacc tgcccagtac
    22141 gaacgagaat acgcaacggt tgaaaactct agtgaacgag tggagtatgc catcaagtta
    22201 cccggacaag gcgaccaaga atacgtctat cttccaattg actctaagtt tccactggca
    22261 gattattacc gcttggaaga agcctatgag acaggtgaca aggatgagat tgaacgctgt
    22321 cgtaagtcac tcctagcaag cgtcaagcgc tttgccaagg atatcaagag caagtacata
    22381 gcaccgcctc ggacaaccaa ctttggaatc ttgtttgttc cgacagaagg tctttattca
    22441 gagattgtgc gcaatccggt cttctttgat gatttgagac gggaagaaca gattattgtt
    22501 gcaggaccaa gtaccctatc agcccttctt aactccctat cagttggttt caagaccctt
    22561 aatatccaaa agagttccga ccatatcagc aagactcttg ccagtgtcaa gaccgagttt
    22621 ggcaagtttg gtggtattct ggtcaaggca caaaaacatc tccaacatgc ctctggcaat
    22681 attgatgaat tattaaaccg tcgtaccata gctatcgagc ggacgctccg tcacattgag
    22741 ttgtcagaag gtgagcctgc gcttgatcta ctctattttc aagaaaatga ggaagaatat
    22801 gaagattagt cacatgaaaa aagatgagtt atttgaaggc ttttacctaa tcaaatcagc
    22861 tgacctgagg caaactcgag ctgggaaaaa ctacctaacc tttaccttcc aagatgatag
    22921 tggcgagatt gatgggaagc tctgggatgc ccaacctcat aacattgagg cctttaccgc
    22981 aggtaaggtt gtccacatga aaggacgccg agaagtttat aacaataccc ctcaagtcaa
    23041 tcaaattact ctccgcctgc ctcaagctgg tgaacccaat gacccagctg atttcaaggt
    23101 caagtcacca gttgatgtca aggaaattcg tgactacatg tcgcaaatga ttttcaaaat
    23161 tgaaaatcct gtctggcaac ggattgtccg aaatctctac accaagtatg ataaggaatt
    23221 ctactcctat ccagccgcca agaccaacca ccatgccttt gaaacgggct tggcttatca
    23281 tacggcgacc atggtacgtt tggcagacgc tattagcgaa gtttatcctc agctcaataa
    23341 gagcttactc tatgcgggga ttatgctgca tgacttagct aaggttatcg agttgacggg
    23401 gccagaccag acagagtaca cagtgcgagg taatcttctt ggacatatcg ctctcattga
    23461 tagcgaaatt accaagacag ttatggaact cggcatcgat gataccaagg aagaagtcgt
    23521 tttgcttcgt catgtcatcc tcagtcacca cggcttgctt gaatatggaa gtccagtccg
    23581 tccacgtatt atggaggcag agattatcca tatgattgac aatctggatg caagcatgat
    23641 gatgatgtca acagctctgg ctttggtgga taaaggagag atgtccaata aaatcttcgc
    23701 tatggataat cgttccttct ataaaccaga tttagattga taatttaaga aaaatgagca
    23761 ttttttagga taagaatgtt cgttttttta tgtgaatatg gtataataag taaaagacaa
    23821 aaatgaatac tcttcgaaaa tctcttcaaa ctagggtagt atcgccttgt cgtatgtata
    23881 tatgcaggta tattacaggg tttgtcagtt ctatcgacaa tctcaaagca gtgttttgaa
    23941 ccaccagcga ccagctttct agtttgcttt ttgatttttt gaataaaaat ggaataggaa
    24001 atagaaatga aattaagaag aagtgatcgg atggttgtca tttccaacta tttgattaat
    24061 aatccttata aactaactag tctcaatact tttgctgaaa agtatgagtc tgctaaatca
    24121 tccatctcag aagatatcgt cattatcaaa cgcgcctttg aggaaattga aatcggtcat
    24181 atccagacag tgactggggc tggcggaggt gtcatcttca caccgtctat ttcgagtcag
    24241 gatgctaagg aaatggttga agacttgcgt accaagttgt cagaaagtga ccgtatcttg
    24301 ccaggtggtt atatctatct gtctgatttg cttagcacac cagccatctt gaaaaatatt
    24361 ggtcgtatta ttgccaaaag ctttatggac caaaaaattg acgcggttat gaccgtagca
    24421 actaagggtg tgccacttgc aaatgcagtt gccaatgtcc tcaatgtctc ttttgtcatt
    24481 gtgcgccgtg acctgaaaat taccgaaggt tcaactgtta gcgtcaacta tgtttcaggt
    24541 tcaagtggtg accgtatcga gaaaatgttc ctttcaaaac gtagtcttaa ggcaggtagc
    24601 cgtgtcttga ttgtggatga cttcttgaaa ggtggcggaa cggtcaatgg tatgattagt
    24661 ctcttgcgcg agttcgactc agaactggca ggtgtagcgg tctttgcgga caatgcccaa
    24721 gaagaacgtg aaaagcagtt tgactacaag tcactcttga aggtaactaa tattgatgtc
    24781 aagaaccaag ccatcgatgt tgagattggc aatatctttg acgaagataa ataagagata
    24841 gaactaaagg ttggaacgat tgtcccagcc tttctttgca aacagaatag aaggaagctt
    24901 atgaaaacac catttatcaa tagagaagag ttagaagcga ttgttgccga gttcccgact
    24961 ccctttcact tgtatgatga gaaggggatt cgtgagaagg caagagctgt caaccaagct
    25021 ttttcgtgga acaagggctt taaggaatat tttgcagtta aggctactcc aactccagct
    25081 attttgaaaa ttctccaaga agaaggttgt ggtgtggact gctctagtta tgtagagctt
    25141 ttgatgagcc ataaactgga ctttctgggt tctgagatta tgttctcttc caacaacacg
    25201 ccagacaagg aatacgccta tgcacgtgaa ttgggtgcga ccattaactt ggatgccttt
    25261 gaagatattg aacatctgga gagagtagca ggcattccag aaatcatctc ttgtcgttat
    25321 aatcctggag gcgtttttga actggggaca gacattatgg acaatcctgg ggaggctaag
    25381 tttggcatga ccaaggacca gctctttgaa gcctttgcta tcttgaagga aaaaggagcc
    25441 aagacttttg ggcttcactc cttcctagcg tccaataccg tgacccatct ctattatcca
    25501 gagttggctc gtcagctctt tgaactggct gttgaaatca aggaaaagtt gggcatttcg
    25561 ttagacttta tcaatctttc tggcggtatt ggtgttaatt atcgtccaga ccaggagccg
    25621 aacgatatcg ccttgattgg tgagggagtt tgtaaggtgt atgaagaagt tcttacgtca
    25681 gcaggtcttg gtcaggtcaa gattttcacc gaattgggtc gttttatgct ggcacctcac
    25741 ggtgctctag tcacaagagt cactcataag aaggaaacct accgtaccta tctaggtgta
    25801 gatgcctcag cagtcaacct catgcgtcca gctatgtacg gagcttacca tcatattagc
    25861 aacgtgaccc atccagatgg accagctgaa gcggtagatg tggttggttc actctgtgaa
    25921 aacaatgata aatttgcagt caatcgcgaa ctgcctcata cagaaatcgg tgatttgctg
    25981 gtaattcatg atacaggtgc ccacggattt tcaatgggct accagtataa tgccaaatta
    26041 cgttctgcgg aaatcctcta taccgaagaa ggtaaagccc gtcaaatccg ccgtgcagag
    26101 cgccctgagg actattttgc aaccttatat ggcttcgatt ttgaagaata atctgataat
    26161 agattgaaaa tgaaattgaa aaacagattg ctttctaaaa aataggcaaa aatcttgttt
    26221 ttcctttaag tcgtgatata ataaaactat aaaacgtttt caaggaaggt aacgatatgt
    26281 ctgaagaaac aattgattat ggacaagtga caggaatggt gcattcgaca gaaagctttg
    26341 ggtcagtaga tgggcccggt attcgcttta ttgtcttttt gcagggctgt cacatgcgtt
    26401 gccagtattg ccacaaccca gacacttggg ctatggagtc caataagtca cgtgaacgga
    26461 cggtagatga tgtcttgaca gaggccttgc gctaccgtgg tttctgggga aataagggtg
    26521 ggattacagt cagtggagga gaagctctct tgcagattga tttcctgatt gccctcttca
    26581 ccaaggctaa ggaacaagga atccactgta ccttggatac ctgtgctctt cctttccgta
    26641 ataaaccacg ttaccttgag aagtttgaca aactcatggc tgtcactgac ttggttcttt
    26701 tggatatcaa ggaaatcaac gaagaacagc acaagattgt cactagccaa actaataaaa
    26761 atatcttggc ttgtgcccag tatctatcag atattggaaa acctgtctgg attcgccacg
    26821 tgctagttcc aggattgaca gacagagatg atgacttgat tgaacttggt aagttcgtca
    26881 agaccctcaa aaatgttgat aagtttgaaa ttctacctta tcacaccatg ggtgagttca
    26941 agtggcgtga acttggaatt ccatattccc tcgaaggagt caaaccacca acagcagatc
    27001 gcgtcaagaa cgctaaaaaa ctcatggata ctgaaagtta tcaagattat atgaaacgtg
    27061 tacatggata gaaaagaagc ctgatggaaa catcgggctt ttgatttgca aaaagactta
    27121 gcaaatcagc taagcctttt tcttcttatc tcgaacgttg ttttccagcg ttgcgatttt
    27181 tgtgtttttt cttgcttgtg atagcagttg gttgttcagg ggtaacgtct tttcgtccac
    27241 ttggtttaga gaaagcactt gcttttggtg ggttcttggc tagttcttca cggacttttt
    27301 tgcgaagttt tggacgaacg atatagttga cgataaactg ttggagaatc atcatgaaac
    27361 caccgacaac ccagtaaagt gtgacactag ctggtgagaa gagggagaag acgacgatca
    27421 tgagtgggct catgtaaatc attttcttga tttgttctct ttgcatttca tcttctactc
    27481 cgtgaagtga aaggagcgat tgaagatagt aaaggacacc agcacaggca accaaaatca
    27541 tacttggaga acctagagga atgcctaggt agcttgcttg agcaacccct tcagtatgtt
    27601 gggcagcaaa gtagatagca gagaagaaag gcatttgaag gaggataggg aaacatccta
    27661 caccaccaaa catgctgatg ccgtgctctt tttgagcggc aaagagggct tgttgggctt
    27721 cgagtttttc ttcttgagta gttgcttctt tgagacgtgt ttggtgtggc tcaaggacat
    27781 gcttgagagc gttcatcttt tcagagtgaa gcgttgcctt ccatgattgg tagataccaa
    27841 gtggtaagat aatcaagcgt acgataatgg ttacgataat gatagcgaca ccaaagccta
    27901 gacctttatc agtagcgaag tacttgatag cttcagccat aggcgctccg atcgtattcc
    27961 aaataaatcc tgttggctga cctgtggttt tatcgacatt gacacagcca gtcaagacaa
    28021 gcaacatagc cactcccata gccgagaatg caaaacgttt aatagatttc actgttttta
    28081 ttccttcttt aaaaattata cctttctatt ctactgtttt tttacaaaat atacaatagt
    28141 tctggagacc taatttgcga ttttaaagtc agagtaggga ggaaagttgc ttagtttgac
    28201 atctaagtag cttacttttg aaaaaggtgt cggtcctttt cggatttctt ggataaattt
    28261 tgccatgata gcagatgagt ctgcttgggc taagatttcc actgtgccat cgtcgttatt
    28321 ccatactcga cctgtgatac caccaatttc aagtgccaag ctgtaaacac cccaacgaaa
    28381 gccgactccc tgtaccctac cttgggcaat cattctaacc ttttgcatac caaacccctt
    28441 tgattgtgtt ataatatttc tatgactatt ataacctcaa aagccaattc tgtggtaaaa
    28501 aatgccaaga aattacgcca aaaaaaatac cgcaagtctg cctatttgat tgaaggctgg
    28561 cacttgtttg aagaagctgt ccaagctgga gtgacgattg agaaaatctt tgccctagaa
    28621 aattatcgag atcagttagc tactttttcg caaactgtct gggtgtcaga ggatattttg
    28681 ctagatttgg cggattctca gactccacag ggaattgttg ccgtggttca aaaagaagaa
    28741 gtaggacaag ctgatttgag tcagggcaag ttcttgtttt tggaagatgt gcaagatcct
    28801 ggtaatgtag gaactatcat tcgaactgcg gatgcagcag gttttacagg agtgattgtt
    28861 tcagataagt cggcagacat ctacagtctc aaaaccctac gttccatgca aggtagtcat
    28921 ttccatctgc ccatttaccg gatgactagt caagcgcttc ttgacgaaac taaaaaggta
    28981 gctatcccag tgctagcaac aaccctatct aaagattctg ttgattacag agaactgcct
    29041 tctatagaaa attttgtact agttatggga aatgagggtc aaggaattag tccccttatg
    29101 gctgaaagtg cagaccagtt ggtccatatt agcatgaagg ggcaggccga gagtttgaat
    29161 gttgcagttg cagccggtat tttaatcttc catttaagct aattttaact ttttttgtta
    29221 taatcaagga aagatgttca cagaaaagga gaaaaggatg aatcacacta ttatacatga
    29281 ccgtgcaggt ctcaatcaat tttacgctaa ggtttatgcc tttgttggtc tgggaatcgg
    29341 actatctgct ttggtatcag gccttatgtt gacggtcttt cagtctcagt tggtttactt
    29401 gttgatgcag gggcgtctct ggttgaccat tgctactttt gcggagttgg ctttggtttt
    29461 cgttgccagt agcatggctt caagaaatag tccagctgct cttccagtat ttttacttta
    29521 ctccgtttta aacggcttta cccttagttt tgtggtggct ttctatactc cgggtacagt
    29581 tttatctgcc tttgtatcga gcgcccttct cttctttgtc atggcggcag ttggtatctt
    29641 taccaagaaa gacttgagtg ggattggtcg agcaatgatg gcagctctca ttggtttact
    29701 gattgctatg gtagtcaata ttttcttggc tagtggcttc tttgattata tgattagcgt
    29761 agccatggtt ttggtttttt cagggttgat tgcttgggat aaccaaaaga ttcgcctcgc
    29821 ctatgaacaa tcacaaggtc gtgtagcgac aggctgggtg gtgtcaatgg ctctcagcat
    29881 ctatcttgat tttatcaatc ttttcctaag tattctacgt atctttggtc gcaatgacta
    29941 aaaggtctat gaaatcagtg ttttgagtga cactgatttt ttttaaattt tctcttttct
    30001 tttcttggaa ataggtgtat aatacgttta ttaaattttt gaggagttgt ctatgaagaa
    30061 aagttttatc catcaacaag aagaaatttc ctttgtcaaa aacactttta cccagtattt
    30121 gaaagataag ctagaagttg tcgaagttca aggtcctatc ttgagtaagg tcggtgacgg
    30181 aatgcaggac aacctgtctg gtgtggaaaa tccagtatcg gtcaaggttc tccaaatccc
    30241 tgatgctact tatgaagtgg tgcactcact tgctaaatgg aaacgccaca ccttggctcg
    30301 ttttggcttt ggtgaaggag agggtctctt tgtccacatg aaagcccttc gtccagatga
    30361 ggattccttg gatgcaaccc actctgttta tgttgaccag tgggactggg agaaggttat
    30421 cccaaatggt aagcgtaaca tcgtttatct aaaagaaaca gttgagaaga tttataaggc
    30481 tattcgcctg actgagctag ctgttgaagc tcgctatgac atcgagtcta tcttgccaaa
    30541 acaaatcacc tttatccata cagaagaatt ggtagaacgc tatccagact tgacatcgaa
    30601 agaacgtgaa aatgcgattt gtaaagaatt tggagccgtc tttttgattg gtatcggtgg
    30661 cgagttgcca gatggtaaac cgcacgatgg acgtgcacca gactatgatg actggacaag
    30721 cgagtctgag aatggctaca agggtctaaa tggtgatatt cttgtctgga atgagtcttt
    30781 aggtggagcc tttgagttgt cttctatggg aattcgtgta gatgaagaaa ctcttagacg
    30841 tcaggtggaa attacaggtg atgaagaccg cttggaattg gaatggcaca agtctttgtt
    30901 gaatggtcta ttcccattga caatcggtgg aggaattgga caatctcgta tggccatgtt
    30961 cctacttcgc aagagacaca tcggagaagt gcaaacaagt gtttggcctc aagaagtccg
    31021 cgatacttac gaaaatattt tgtagagaat cgaaccgcaa ggttcggttt tctttctctt
    31081 tttgtctata atttggtata ataaacagta tgaaaatcgt atcaggaatc tatgggggac
    31141 gtcccctcaa gacactagaa ggcaagacga caagacctac ttcggataag gttaggggag
    31201 ccatttttaa catgattggt ccctactttg aagggggacg agtcttggac ctttatgcag
    31261 gtagtggtgg tttatctatc gaagcagtat cgcgtggcat gtccagtgct gttttggtgg
    31321 agcgagaccg taaggctcag accatcgtgg ctgaaaatat ccagataacc aaggaaattg
    31381 gaaaatttca actcctcaag atggatgcag aaagggcatt ggaacaggta tctggggaat
    31441 ttgacctcgt tttcttagac cctccctata ccaaggaaca aatcgtagca gatattgaaa
    31501 aaatggctga gagagagctt ttttctgaag atgttatggt tgtgtgcgag acggataagg
    31561 ccgttgaact tccagaagaa attgcctgtc tgggtatctg gaaggaaaag atttatggaa
    31621 ttagtaaggt gacagtctat gtcagataag attggcttat tcacaggctc atttgatccg
    31681 atgacaaatg ggcatctgga tatcattgaa cgggcgagca gactctttga taagctctat
    31741 gtcggtattt tttttaatcc ccacaaacaa ggatttcttc ctatcgaaaa tcgtaaacgg
    31801 gggctagaaa aggctttggg acatctggaa aatgttgaag tcgtggcttc tcatgatgaa
    31861 ttggtggtcg atgttgcaaa aagattgggt gctacttgtc tagtgcgtgg tttgaggaat
    31921 gcggcggatt tgcaatatga agctagtttt gattactaca atcatcagct gtcttctgat
    31981 atagagacta tttatttaca tagtcgacct gaacatctct atatcagttc atcaggcgtt
    32041 agagagcttt tgaagtttgg tcaggatatt gcctgctatg ttcccgagag tattttggag
    32101 gaaataagaa atgaaaaaaa agattagatg gcccttatac gtcattgcgg ccttgattgt
    32161 gactttcttg gcatttgtag tgcccttgcc ttattatata gaggttccag gtggttcgga
    32221 agatattcgc caagtcctta aaataaatga cacagaagat aaggaagctg gtgcctatca
    32281 attcgttacg gttggtgttc aacatgccac tttagctcat atgatttatg cttggttgac
    32341 accttttaca gatattcgta gtgctcagga gactacaggt ggttcttccg atgttgaatt
    32401 tatgcgaatc aatcaattct acatgcaaac atcgcaaaat atggccaagt atcaaggact
    32461 aaaaacagct ggtaaggata tcgaactcaa gtactttgga gtttatgttt tgaatgtgac
    32521 ggataattca acctttaaag ggattctcaa tatctctgat acagtcacag cagtcaatga
    32581 tcagaccttt gatagttcca aagacttgat tgattacgtc agttctcaaa aattagggga
    32641 ttccgtcaag gtcacctatg aagaggatgg gcaaaccaag tctgcagaag gaaagattat
    32701 caccttggaa aatggcaaaa atggaattgg aatcggcttg attgaccgta cagaggtaat
    32761 cagcaatgtc ccaattagct tttcaacagc tggtattggc ggtccaagtg ctggtctcat
    32821 gtttagtcta gctatctata ctcaaatagc tcacccagat cttcgtaatg gtcgtattgt
    32881 tgccggtaca ggtaccattg accgcgatgg taatgtggga gacattggag gtattgataa
    32941 gaaggttgta gcttcggcta gggcaggtgc tgctattttc tttgctcctg ataaccctgt
    33001 tagcgaagaa gaacaaaagg cgcatccgga cgcgaaaaac aactaccaaa cagccctaga
    33061 agcggctaaa acaatcaaga cggatatgaa aatcgtgccc gttaaaaccc tacaagatgc
    33121 gattgattac ttgaaaaaca atccctaatt tttgagtgaa gtttccaaac tagcgcacaa
    33181 acagagaaga tggtataata gtcaaatggt tcaattatta ttcactctaa gtagtcatat
    33241 gctctttatt tatgtgagtt tttacctttt aaaggatctt gttagatggg agaaggtttt
    33301 aaaagtgaca gatgagaata caagaaaagt tcgtttatta gtagcctttt ttagcattgt
    33361 cataggctac atcctgagtt ctttctttat tagcctgtat catttgtggc aagaagcgct
    33421 tagaggatta ttatgaaatc aagagtaaag gaaacgagta tggataaaat tgtggttcaa
    33481 ggtggcgata atcgtctggt aggaagcgtg acgatcgagg gagcaaaaaa tgcagtctta
    33541 cccttgttgg cagcgactat tctagcaagt gaaggaaaga ccgtcttgca gaatgttccg
    33601 attttgtcgg atgtctttat tatgaatcag gtagttggtg gtttgaatgc caaggttgac
    33661 tttgatgagg aagctcatct tgtcaaggtg gatgctactg gcgacatcac tgaggaagcc
    33721 ccttacaagt atgtcagcaa gatgcgcgcc tccatcgttg tattagggcc aatccttgcc
    33781 cgtgtgggtc atgccaaggt atccatgcca ggtggttgta cgattggtag ccgtcctatt
    33841 gatcttcatt tgaaaggtct ggaagctatg ggggttaaga ttagtcagac agctggttac
    33901 atcgaagcca aggcagaacg cttgcatggt gctcatatct atatggactt tccaagtgtt
    33961 ggtgcaacgc agaacttgat gatggcagcg actctggctg atggggtgac agtgattgag
    34021 aatgctgcgc gtgagcctga gattgttgac ttagccattc tccttaatga aatgggagcc
    34081 aaggtcaaag gtgctggtac agagactata accattactg gtgttgagaa acttcatggt
    34141 acgactcaca atgtagtcca agaccgtatc gaagcaggaa cctttatggt agctgctgcc
    34201 atgactggtg gtgatgtctt gattcgagac gctgtctggg agcacaaccg tcccttgatt
    34261 gccaagttac ttgaaatggg tgttgaagta attgaagaag acgaaggaat tcgtgttcgt
    34321 tctcaactag aaaatctaaa agctgttcat gtgaaaacct tgccccaccc aggatttcca
    34381 acagatatgc aggctcaatt tacagccttg atgacagttg caaaaggcga atcaaccatg
    34441 gtggagacag ttttcgaaaa tcgtttccaa cacctagaag agatgcgccg catgggcttg
    34501 cattctgaga ttatccgtga tacagctcgt attgttggtg gacagccttt gcagggagca
    34561 gaagttcttt caactgacct tcgtgccagt gcagccttga ttttgacagg tttggtagca
    34621 cagggagaaa ctgtggtcgg taaattggtt cacttggata gaggttacta cggtttccat
    34681 gagaagttgg cgcagctagg tgctaagatt cagcggattg aggcaagtga tgaagatgaa
    34741 taagaaatca agctacgtag tcaagcgttt acttttagtc atcatagtac tgattttagg
    34801 tactctggct ctaggaatcg gtttaatggt aggttatgga atcttgggca agggtcaaga
    34861 tccatgggct atcctgtctc cagcaaaatg gcaggaattg attcataaat ttacaggaaa
    34921 ttaggctgga gaaccagcct ttttctaaag ataaggagaa atatgaacaa aaaaacaaga
    34981 cagacactaa tcggactgct agtgttattg cttttgtcta cagggagcta ttatatcaag
    35041 cagatgccgt cggcacctaa tagtcccaaa accaatctta gtcagaaaaa acaagcgtct
    35101 gaagctccta gtcaagcatt ggcagagagt gtcttaacag acgcagtcaa gagtcaaata
    35161 aaggggagtc tgaagtggaa tggctcaggt gcttttatcg tcaatggtaa taaaacaaat
    35221 ctagatgcca aggtttcaag taagccctac gctgacaata aaacaaagac agtgggcaag
    35281 gaaactgttc caaccgtagc taatgccctc ttgtctaagg tcactcgtca gtacaagaat
    35341 cgtaaagaaa ctgggaatgg ttcaacttct tggactcctc caggttggca tcaggtcaag
    35401 aatctaaagg gctcttatac ccatgcagtc gatagaggtc atttgttagg ctatgcctta
    35461 atcggtggtt tggatggttt tgatgcctca acaagcaatc ctaaaaacat tgctgttcag
    35521 acagcctggg caaatcaggc acaagccgag tattcgactg gtcaaaacta ctatgaaagc
    35581 aaggtgcgta aagccttgga ccaaaacaag cgtgtccgtt accgtgtaac cctttactac
    35641 gcttcaaacg aggatttagt tccctcagct tcacagattg aagccaagtc ttcggatgga
    35701 gaattggaat tcaatgttct agttcccaat gttcaaaagg gacttcaact ggattaccga
    35761 actggagaag taactgtaac tcagtaaaag ataagcctaa actcctatgt cacttatgga
    35821 tgtaggagtt ctttttacta gtttaagcag ggctagaaca ggtactaaga aaaaatagca
    35881 acttctaaaa ctaacttcca gttttgggag agagatggaa gttactttga gaagttacgg
    35941 taagaaaaaa taatatgtat tggattaaat tttgtgatat aataaaggat aagatactat
    36001 tttaggagaa gaactatgga agacccaagt agtcagaatt tgttgctaca atttgtttta
    36061 ttgtttatct tgacggtatt aaatgccttt ttctcagcca cagaaatggc catggtttct
    36121 cttaatcgtg cccgagttga acaaaaggcg gaagaaggag acagacgcta catccgcctg
    36181 ctgaaggtac tagaaaatcc taaccacttt ttatcaacca ttcaggtagg aatcaccctg
    36241 attacaatct tatcaggggc gagtttaact aatactctag gacgtgtgat tgcctcttgg
    36301 cttgggaatg gcgaaacagc acaagccgtg gcaacttttc tatccttggc atttttgacc
    36361 tatatttcca tcgtttttgg ggaattatat cctaagagaa tcgctcttaa tctaaaggat
    36421 gccttggcaa ttcgtacagc gccgattatc attgggatcg gaaaactagt cagtcctttt
    36481 gtttggcttt tagctgcttc gaccaatttc ttgagccatt tgactcctat gtcattcgat
    36541 gatgcggatg aaaagatgac ccgtgatgaa attgcctata tgctgacaaa tagtgaagaa
    36601 acattggatg ctgatgagat tgagatgcta caaggtgtct tttcgctcga tgaactgatg
    36661 gcacgagagg ttatggtgcc tcgaacggat gcctttatgg tggatattca ggatgatagt
    36721 caagccatta tccaaagtat tttaaaacaa aattattctc gtatcccggt ttatgatggg
    36781 gataaggaca atgtaattgg aatcattcac accaagagtc tccttaaggc aggctttgtg
    36841 gacggttttg acaatattgt ttggaagaga attttacaag atccactttt tgtacctgaa
    36901 actatttttg tggatgactt gctaaaagaa ctgcgaaata cccaaagaca aatggcaatc
    36961 ttgctggatg aatacggtgg tatggctggt ttggtgactt tggaagacct cttagaggaa
    37021 attgttggtg aaattgatga tgaaacggac aaagcagcaa tagatgttca tcagattggc
    37081 gaggatacct acattgttca aggaactatg accctcaatg atttcaataa ttactttaat
    37141 gttgaactgg aaagtgatga cgttgatact atcgctggtt attatttgac aggagtggga
    37201 accattccaa cgactgagaa actcagctat gaattggtca gccaaaacaa acagtttatc
    37261 ttaaccaatg ataaagtgaa gaatggacgt gttaccaagg taaaagtcca aatcacagaa
    37321 gtcgaaatag aagaagaaac agaataaact agaggctttt gtcaccaggc gtgacgaagc
    37381 ttttttcaat tgatttcaaa agggaattat gccatggaaa gaactctgcc acaaactcgc
    37441 tccaaaagtt tttaaagtta tacgtattta ttcgagggaa aataagaaaa gcccttcaaa
    37501 ttgggctttc tgttcatttg aaacctgata aatcaagtta tagaaggcgg tagacggatt
    37561 tgaaccgacg atcaagcttt tgcagagccg tgccttacca cttggctata ccgccttaac
    37621 gtttattatt ataccttgaa aatgattttc agtcaatagt tttttcagtt ttttgtacca
    37681 tagcgtgatt cgaatgtgat gccaagtttt ctatgggaaa aaatttacca aaaacctact
    37741 gaaacccctt gaaaaatcta gctaaatagg gtataatatg ggtaatcatt tgtcgtaggt
    37801 tttgtctgaa atattgtcca gacaaggctc acagcagtta aatcttctga aaaagtcaga
    37861 tttagctgct ctttttgtgc tttttttcaa gattttgagc atttgtaaca gaggcttaaa
    37921 gattctgaaa attcgtcaag aggacacggt gataaggggt ttacaaccat atgacgatta
    37981 gaaaagcctg attgataagg cttggaactt atttacaaag gagaatcatc ttggcaggac
    38041 atgacgttca atacgggaaa catcgtaccc gtcgtagttt ttcaagaatc aaagaagttc
    38101 ttgacttacc aaatttgatt gaaattcaaa ctgactcatt caaagctttc ctagaccacg
    38161 gtcttaagga agtgtttgaa gatgtattgc caatttcaaa cttcacagac acaatggagt
    38221 tggaatttgt tggatatgaa atcaaggaac caaaatacac gctagaagaa gctcgtatcc
    38281 acgatgctag ctactcagca ccaatttttg taaccttccg tttgatcaat aaagaaacag
    38341 gcgaaatcaa gacccaagaa gttttctttg gtgatttccc aatcatgaca gaaatgggta
    38401 ctttcatcat caatggtggt gaacgtatta tcgtttctca gttggtccgc tcaccaggtg
    38461 tttactttaa cgacaaagta gacaaaaatg gtaaggtggg ctatggttca actgttatcc
    38521 ctaaccgtgg agcttggttg gaacttgaaa gcgactcaaa agatatcacc tacactcgta
    38581 tcgaccgtac tcgtaagatt ccatttacaa ccttggttcg tgctcttggt ttctcaggtg
    38641 atgatgaaat ctttgatatc tttggtgaca gcgaattggt tcgcaacact gttgaaaaag
    38701 atatccacaa gaatccaatg gactctcgta cagacgaagc cttgaaagaa atttacgaac
    38761 gccttcgtcc aggtgagcct aagacagctg aaagctcacg tagcttgctt gtggctcgct
    38821 tctttgaccc acgtcgctat gacttggcag cagttggtcg ttacaaaatc aataaaaaac
    38881 tcaatgttaa aacacgtttg ctcaaccaaa ccattgcaga gccattggta gaccctgaaa
    38941 ctggagaaat cttggtagaa gctggtacga ttatgactcg tagcgtgatt gaaagcattg
    39001 aaagccattt ggatggcgac ttgaacaaga ttgtctacat cccaaacgat gcagccgttg
    39061 tgactgagcc tgttgttctt caaaaattca aggttgttgc tccaactgat ccagatcgcg
    39121 tcgtaacgat cattggtaat gctaacccag atgacaaggt tcgtacggtg actcctgcag
    39181 atatccttgc tgagatgagc tacttcctca acttggctga aggacttggc cgtgtagatg
    39241 atatcgacca ccttggaaat cgtcgtatcc gtgcggttgg tgaattgctt gccaaccaag
    39301 tacgtttggg actttctcgt atggaacgta atgtccgtga acgtatgtct gttcaggaca
    39361 atgaagtctt gacaccacaa caaattatca atatccgtcc tgtaacagct gcagttaaag
    39421 aattctttgg ttcatcacag ttgtcacagt tcatggacca acacaacccg ctttctgagt
    39481 tgtctcacaa acgccgtttg tcagccttag gacctggtgg tttgactcgt gaccgtgctg
    39541 gatatgaagt acgtgacgtg cactacactc actatggtcg tatgtgtcca atcgagacac
    39601 ctgaaggacc taacatcggt ttgatcaata acttgtcatc ttacggacac ttgaacaaat
    39661 atggttttgt tcaaacacca taccgtaagg ttgaccgtga aacaggtgtt gtcacgaacg
    39721 aaattgtttg gttgacagct gatgaagaag atgaatatac tgtagctcag gctaactctc
    39781 gtctgaatga agatggaacc tttgctgaga agattgtcat gggacgtcac caaggggtca
    39841 accaagagta tccagctaat attgttgact acatggacgt ttcaccaaaa caggtagttg
    39901 ccgttgcgac agcatgtatt cctttcttgg aaaacgatga ctccaaccgt gccctcatgg
    39961 gagccaatat gcaacgtcag gctgtgccat tgattaatcc tcaggcacct tacgttggta
    40021 ctggtatgga ataccaagca gcccacgatt ctggtgcggc tgtgattgct cagtatgatg
    40081 gtaaagttac ttacgcagat gctgacaagg tagaagttcg tcgtgaagat ggatcattgg
    40141 atgtttacca catccaaaaa ttccgtcgtt caaactcagg tactgcttac aaccaacgca
    40201 ctctcgtaaa agttggtgac gtcgttgaaa aaggcgattt catcgctgac ggaccttcta
    40261 tggaaaatgg agaaatggcg cttggacaaa acccaatcgt tgcctacatg acttgggaag
    40321 gttacaactt cgaggatgcc gttatcatga gcgaacgctt ggtgaaggac gatgtctaca
    40381 catctgttca ccttgaagaa tacgaatcag aaacgcgcga tacaaagctt gggcctgaag
    40441 aaatcactcg cgaaattcca aacgttggtg aagatgccct caaagacctt gacgaaatgg
    40501 ggattatccg tattggtgct gaggttaaag aaggtgatat tcttgtaggt aaagtaacac
    40561 ctaagggtga gaaagatctt tcagctgaag aacgtctctt gcacgctatc tttggagaca
    40621 agtctcgtga agtgcgtgat acttctcttc gtgtaccaca cggtgccgat ggtgtcgttc
    40681 gtgatgttaa gatctttaca cgtgtaaatg gagatgagtt gcaatcaggt gttaacatgt
    40741 tggttcgtgt ttacatcgct caaaaacgta agattaaggt cggagataaa atggccggac
    40801 gtcacggaaa caaaggggtt gtctctcgta tcgttcctgt agaagacatg ccttaccttc
    40861 cagacggaac tccagtcgac atcatgttga acccacttgg ggtgccatca cgtatgaata
    40921 tcggtcaggt tatggagctt caccttggta tggcagctcg tactcttggt attcacattg
    40981 cgacaccagt ctttgatgga gcaagttctg aagatctttg gtcaactgtt aaagaagcag
    41041 gtatggatag cgatgccaag acaatccttt acgatggacg tacaggtgaa ccatttgata
    41101 accgtgtttc tgttggagtc atgtacatga tcaaactcca ccacatggtt gacgataaat
    41161 tgcatgcgcg ttcagtcgga ccttactcaa ctgttaccca acaaccactc ggaggtaaag
    41221 ctcagtttgg tggacaacgt ttcggtgaga tggaggtttg ggctcttgaa gcctacggtg
    41281 cgtcaaatgt ccttcaagaa atcttgactt acaagtcgga cgatatcaac ggacgtttga
    41341 aagcctatga agctattaca aaaggcaaac caattccaaa accaggtgtt ccagaatcct
    41401 tccgagttct tgtcaaagaa ttgcaatctc ttggtcttga catgcgtgtc ctagacgaag
    41461 atgaccaaga agtggaactt cgcgacttgg atgaaggaat ggacgaagat gtcatccacg
    41521 tagatgacct tgaaaaagcc cgcgaaaaag cagcccaaga ggctaaagca gcctttgaag
    41581 ctgaagaagc tgagaaagca acaaaagcgg aagcaacaga agaagctgct gaacaagaat
    41641 aagcagttca cttagaatag aaagggaaga aatagtggtt gatgtaaatc gttttaaaag
    41701 tatgcaaatc accctagctt ctccaagtaa agtccgttca tggtcttatg gagaagtcaa
    41761 aaaacctgaa acaatcaatt atcgtacctt gaaaccagaa cgtgaaggac tctttgatga
    41821 agtgatcttt ggtcctacaa aagactggga atgtgcttgt ggtaagtaca aacgcattcg
    41881 ttacagagga attgtttgtg accgctgtgg ggttgaagta acgcgtacga aagttcgtcg
    41941 tgagcgtatg ggacatatcg aattgaaagc tcctgtatct cacatctggt acttcaaggg
    42001 gattccaagc cgtatgggct tgacccttga tatgagccct cgtgccctcg aggaagttat
    42061 ctactttgcg gcttatgtgg tgattgatcc taaggataca ccacttgagc acaagtctat
    42121 catgacagag cgcgaatacc gagagcgctt gcgcgaatat ggttatggtt catttgttgc
    42181 caagatgggt gccgaagcca tccaagacct tttgaagcaa gtagatcttg aaaaagaaat
    42241 tgctgaactc aaagaagaat tgaaaacagc tactggacaa aaacgtgtca aagccatccg
    42301 tcgtttggat gttttggatg ccttttacaa gtctggaaac aaacctgaat ggatgattct
    42361 taacatcctt ccggttatcc caccagatct tcgtccaatg ttgcagttgg atggtggccg
    42421 ttttgcctca tctgacttga atgaccttta ccgccgtgtt atcaatcgta acaaccgttt
    42481 ggctcgtttg cttgagttaa atgcaccagg tatcatcgtt caaaatgaga aacgtatgct
    42541 tcaagaagca gttgacgctt tgattgacaa tggtcgtcgt ggtcgtccaa tcacaggacc
    42601 aggtagccgt ccattgaaat cattgagcca catgcttaaa ggtaaacaag gacgcttccg
    42661 tcaaaacttg ctcggtaaac gtgttgactt ctcaggacgt tcagttatcg ccgttggtcc
    42721 aactcttaag atgtaccaat gtggtgtgcc acgtgaaatg gcgattgaac tctttaaacc
    42781 atttgtcatg cgtgaaatcg ttgcccgtga tatcgtgcaa aacgtcaaag cagctaaacg
    42841 cttggtggaa cgcggagatg agcgtatctg ggatatcctt gaagaagtga ttaaagaaca
    42901 cccagtgctt ttgaaccgcg caccgaccct tcaccgtttg ggtatccaag ccttcgagcc
    42961 agtcttgatt gatggtaagg ctcttcgctt gcacccactt gtctgtgaag cctacaatgc
    43021 tgactttgac ggggaccaaa tggccattca cgtaccactt tcagaagaag cacaagcaga
    43081 agctcgtatc ctcatgctag ctgctgagca catcttgaac ccgaaagatg ggaaaccggt
    43141 agttactcca tctcaggaca tggttttggg taactactac ttgaccatgg aagaagctgg
    43201 tcgcgaaggt gaaggaatgg tcttcaaaga ccgtgacgaa gcggttatgg cttaccgcaa
    43261 tggttatgtt cacctccact cacgtgttgg tatcgcaaca gacagcctca acaagccttg
    43321 gacagaagag caaagacata aggtcttgct tacaacagtt ggtaaaattc tcttcaacga
    43381 tatcatgcca gaggggctac catacttgca agaaccaaac aatgccaact tgacagaagg
    43441 tgttccagct aaatacttct tgccacttgg tggagatatc aaggaagcta tcagcaatct
    43501 tgagctcaac cctccattca agaagaaaaa cctcggaaat atcatcgctg aaatcttcaa
    43561 acgtttccgt acgacagaaa cttctgccct acttgaccgc atgaagaacc tcggttacca
    43621 ccactcaact cttgcaggat tgacagtggg tattgccgat atcccagtcg ttgatgacaa
    43681 ggctgaaatc attgaagaat cacacaaacg tgtcgaacaa atcaccaaac aattccgtcg
    43741 tggtatgatc acagacgacg agcgttacaa tgctgttaca gctgaatggc gtgctgcccg
    43801 tgaaaaactt gagaaacgct tgattgctaa tcaagatcct aagaacccaa tcgttatgat
    43861 gatggactct ggagcccgtg gtaacatctc aaacttctca cagcttgccg gtatgcgtgg
    43921 tctgatggct gctccgaatg gacgtatcat ggaattgcca atcctttcaa acttccgcga
    43981 aggtttgtcg gtattggaaa tgttcttctc aactcacggt gctcgtaaag gtatgaccga
    44041 tacggccctt aagacagccg actcaggtta cttgactcgt cgtttggtcg acgttgccca
    44101 agacgttatc atccgtgagg acgactgtgg aactgaccgt ggtctcttga tccgttctat
    44161 cgcagaagga aaagagatga tcgagtctct cgaagagcgt ctcaacggtc gttacactaa
    44221 gaaaactgtt aaacatccag aaactggtgc agtgattatt ggtccaaatg aattgattac
    44281 agaagacaag gcgcgtgaaa ttgtcaatgc tggtgtggaa gaagtgacta tccgttctgt
    44341 atttacatgt aacactcgtc acggtgtctg ccgtcactgt tacggtatca acttggcgac
    44401 tggtgatgcg gttgaagttg gtgaagcagt tggtacaatc gctgcccaat ctatcgggga
    44461 acctggtaca cagcttacaa tgcgtacctt ccacacaggt ggggttgcct caaataccga
    44521 tatcactcag ggtcttcctc gtgtccaaga aatctttgaa gcccgcaatc ctaaagggga
    44581 agcggttatc acagaggtta aaggacaagt tactgctatc gaagaggatg catcaactcg
    44641 taccaagaaa gtctttgtta agggtgaaac tggcgaaggt gaatatgtcg ttccatttac
    44701 agctcgtatg cgtgtcgaag ttgggggcca agtagcgcgt ggtgctgctc tgacagaagg
    44761 ttctatccaa ccaaaacgtc tccttgcagt tcgtgatgtc ttgtcagttg aaacgtacct
    44821 tctcggtgaa gtacaaaaag tttaccgtag ccaaggggta gaaatcggtg acaaacacat
    44881 cgaggtaatg gttcgtcaaa tgatccgtaa agtccgtgtc atggatccag gtgatacaga
    44941 tcttctcatg ggtaccctca tggatatcaa tgactttaca gatgccaaca aagatgtcct
    45001 tatcgcaggt ggagttccag cgacaggtcg cccagtcctt atgggaatta ccaaagcctc
    45061 acttgaaaca aacagtttct tgtcagcggc ttccttccag gaaacaactc gtgtccttac
    45121 tgacgcagct atccgtggta agaaagacca tctccttgga cttaaagaaa atgttatcat
    45181 cggtaagatc atcccagctg gtactggtat ggcccgttac cgtaaccttg aaccacatgc
    45241 tgtcaacgaa gaagaatacc ttaatcctcc agtagaggaa gaaggaaatg aagaaacaac
    45301 agaagtagtt gtggatactg ccgttgaaac tgtggaagaa acagtagaat aagagagaat
    45361 gagagaaccc tcgggttctc tcattctctc ttctgaaaac tttctccatt tttccacgaa
    45421 atgtggtaaa ataaaagaaa gaagctgaca aaggagacgg gtatggaaca aacattcttt
    45481 atcatcaaac cagatggtgt aaaaagaggg ctagtgggtg aagtgttaaa gcgcatcgaa
    45541 caacgtggat ttacaatcga aaaattggag tttcgttcac aggtttcaga agagttgatt
    45601 gaccagcact atcaggactt ggttggtcag agtttttacc caccgattcg tgaattcatg
    45661 acttcaggtc cagttcttgt gggtgtcatt tctggtccca aagtaatcga aacttggcgg
    45721 accatgatgg gtgcaactcg tccagaagaa gctttaccag gcactattcg aggtgatttt
    45781 gcaaaagctg ctggagaaaa tgagattatc caaaatgttg tacatggttc agattcagaa
    45841 gagtcagcta agcgagaaat tgctctttgg ttttaagagt ggattggctc aatcaattgg
    45901 ttaaaagctc atttgaatag aaagtatagt caattagttt aagacatgac gcatgatatc
    45961 aaacttttta gtttttgata tggtgcgttt tttgattaag tgactattag tttgtctcgc
    46021 tccgttaggt gcgagacaaa aaaaacaccc gctatgcggg tgcgcgtcga aggttatacc
    46081 aaaaaaactc caaacgcgat acaataaagg tgttcaatcc aattgtaaag cgaaaggaga
    46141 aaaatatggc acaaaaggtt cttttatcct gttcttgttt caattgacta tatttctata
    46201 ctttttcttc ctttctctgt gaaaatcgca agtttttccc accttttctc aatactgaaa
    46261 caacatcttt ccagaacttc aataatttct ttttgtgtta aatcattatt taactaaatc
    46321 tttggaggca atacgttttc aaagctactt ttttaccata ggttttgagc cttcaacaca
    46381 gattactaag ccaaaaatgc tctaaatttt tgaaattcta acaaattcca ctcaacttta
    46441 tcatcatctt tgactttcac aatatcttcc tcaaaataac atggaataaa gatagttttc
    46501 ttttaaaacg gttgtaggag gaaaatctct cctacactat tattgattct gagagaaaat
    46561 tgcaaaatat catctaccaa actttcaatt attaagattt tttgcagttt atctgaatta
    46621 gaatcgcaag gcttctcaat aatataattg tcgaattttt tcattctaac ttcctctcca
    46681 ttatttcttc ctttatcaag aaaaagttac ctcaatatta ataaagtaca attctaaatt
    46741 aactccaatt cttgattcat gataatattt cagtaactca tcgtgcaatt aaaaacaagg
    46801 atttttattt cagatgatcc atcgtgaaca cttcatcagc catctcccaa attgccggat
    46861 tatgtgttgc gataatgatt agcctattat catctcgaag agatagcaaa atctccataa
    46921 tcaactgaga ggttgctggg tctattgaag ctgttggctc atctgccaga ataaagggtg
    46981 gattctttaa gataattttt gccaaggcaa cccgttgcga ttctccgccc gataactcaa
    47041 agatgcgctt atctaggtca agataagcca ggccgacctg ttctaaagcc tgcttctgcc
    47101 tcaaccgctg ttccgaccga ctcaactttt gaccaatgag acctagctta aggttttctt
    47161 caatactttg gttttcaatt aagccaaagt tctggaagag gtagcccaat tcgtgacgga
    47221 aaaaatcact cgatttataa ttggccaagt ctttacctcg gtaaaaaatc gtcccatcat
    47281 aaggttctaa tttcccaatc atgttcatca aggttgtttt tccgctacca cttgaaccaa
    47341 ttaaggcata gacttttcca gcctcaaatg tcattgaaag attcgaaaat aactctcgtt
    47401 ctccaaaaga tttactcacc tgtttaagtt caatcatatt aacctccctt caaaacaaga
    47461 atggacatct tgttttcttt ctgcatttgg acatgtaact gtaaaagaga tagaccagta
    47521 aagagtaaca agactaagaa agcaactcct atctctacct gaagaaatac actcgcaaca
    47581 aatcccagta aaaacacacc cagttgagca aagagataag tgcgatggat ttctaagaac
    47641 ctgagacctg caatgcgttt gataaaaatg gcacgtctaa attcttcaaa gtagagccta
    47701 ttcatagtgt taaacaacaa gattgaagtt gcaatcccaa gcacagctcc tgctagcatt
    47761 acccaacgtt ccctctggat attatccaat aatgtgatgt agttgtggta acctgtttgc
    47821 atttctgaga cccaattttc aatgccttgt ctctggataa gctcctgggc atcagacaat
    47881 tgattaaaga gaacgtagtt ctgtactgcg tctatccaaa acaaaatgga ctgtggacca
    47941 gttgattggg gtgttataac aatgatgatt ggatctttca aaaactgctg gtaagaaata
    48001 ggggtcgtat tatacacaaa acgatcctga cctgattcta aataacctac cgtagcagtc
    48061 atttgctgtc gttcatcttg actagacatg cgactggtta agtcgtcttc aaaaacagat
    48121 ttataatgtt cttcctctga acggaggtgt tcaggcagca ataagacaaa ctcccctaca
    48181 tcaaggtgat tcatcttttg ctcaatagtt gtatctacag gaatgttttg acgctccaag
    48241 tattgcggtg tgacgataag gacattgcca ttcgggctgt aatcgtgcca ttctgtagag
    48301 gttataaagt ttttggagga agccatgcca ttttgcaaag tacggtcaat taactggtgt
    48361 ctagataaga aagccttttg ttctgaaaca gccagatcca tcaattgata ccaagttctg
    48421 agtttccttt gagcttgttc atcaaaacca ggactcgttc cttcacggct gattgatagg
    48481 gtaatcagtt gcctttcctg gctccaagcc tcttgtccaa tccgatgctg ttgccaggct
    48541 tgggaatact ttaagctact ccctattccc agcgttacaa taataatcgc taatagttga
    48601 ccgattaaaa tcaaactaat gattcctctg acaggcactt gcccttttat aatctgcatc
    48661 aggtgtatct tttttatccc aactgcgaag agttgagcga aaaacaggga gatagacaac
    48721 aagatgagat tataactgag caagcctgct cctatggtca ttagggattg cgttggaagc
    48781 gacagaattt tttgcataag aatggtgagc acgccagcta aactgaaacc aacagctatc
    48841 cctttcaaat catcaccaac tggtctaaga aaaatggacc accgtttctc tcctgaaatg
    48901 aggcgaatgc ccgacgaccg taattggcta atttgactaa ttaaagtcag tgctccaaag
    48961 gttaagatga aaatcaatag actgattaac tgaaatccgt tactaaagat agccattaac
    49021 gtagatagtt tagatggaat tgtcaggtgc atattagtca agccaagttg acttagctcc
    49081 tcccttagca agtgaatatc taggtgtcca tcgaatacaa aatagtttgt ttcaacacta
    49141 ctactttgag catcttctag ttttttttcc tgtagcccgt caggcaactt tccgtctcca
    49201 aaggtcgtat aagaaaaaac tgtagtacct tctgagttag gatcttggtg ttgaatcgca
    49261 ataaagctat ctgtttcttc tgctagtttt tccaagcgtg tagcaacatg ctgaaaagtt
    49321 gtttcagggg aagaatcatg tacaacaacg ttggggtaat aatgggagac atatgtatca
    49381 gtccaaatgg taaataccca aacaaagaac aaactagcaa gcaggttgga tatgagtata
    49441 aataattttt tcatagtgca ttccttattc taaaacagag ggcagaaata cccctgccct
    49501 ctataaacta acaagcttac taaactattg ataaaagaat cttcttcaat tattctttac
    49561 catcccgcat caaatgtaat tcgctccccc aaatttgtat taacaaacgc ataggccgca
    49621 ctatgtgctc ctgcataaac tgttttttga ttgtcacgtt cattacttcc aacataggcc
    49681 cagtgatact gtgaaccatg ataataattc gaaaatgctc cccaattata tggatcatga
    49741 tggcctccat aggtccactc gccaccacgg tgagaaactg ccaaaacagg aagagccaaa
    49801 ctaaatgcca aaacggcaag ggatgatgca actacttttc taaattttaa catattatta
    49861 ctcctctaaa tgaaatatta tgaaaatgag cgaaatgcaa ctacatattt taattagaaa
    49921 tctcagtctg aattatagta tcctattttt tcctcctttc taactgcacg ttcctttcta
    49981 caggcagaga cctaatttta taaaccaatc ttaaatactt tagattttgt aactcataat
    50041 tttcaacagt tggttagcta tgaaagcatt ttatcaaaca tctccattct tatatctatc
    50101 tggtattgtt acaagctctc atcagaatac ctttctctaa atagttgaat tttaaagtaa
    50161 tataaaatca atcagccttc ccaacatcaa gaccattgga cttcttttgc gattgcactc
    50221 gaatctccaa ttgtttattc ttctgaataa actgttggta aggaattgta ttactaaaca
    50281 taaaaacacc tagcaatgca aatgtaacta attttttaat tccacgtttt ttccatgcaa
    50341 tgaaacccct ttctttttat tctaacagat atattttatt tagtcaagtg tttttatt
//
LOCUS       DAVKZB010000004        47683 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKZB010000004 DAVKZB010000000
VERSION     DAVKZB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.004"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(282..749)
                     /locus_tag="VXA80_000183"
     CDS             complement(282..749)
                     /locus_tag="VXA80_000183"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV0352745.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(750..1955)
                     /locus_tag="VXA80_000184"
     CDS             complement(750..1955)
                     /locus_tag="VXA80_000184"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV0352746.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(1975..2610)
                     /gene="ribE"
                     /locus_tag="VXA80_000185"
     CDS             complement(1975..2610)
                     /gene="ribE"
                     /locus_tag="VXA80_000185"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV0352747.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVTKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(2595..3695)
                     /gene="ribD"
                     /locus_tag="VXA80_000186"
     CDS             complement(2595..3695)
                     /gene="ribD"
                     /locus_tag="VXA80_000186"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV0352748.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(3829..3943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4100..4693
                     /gene="ruvA"
                     /locus_tag="VXA80_000187"
     CDS             4100..4693
                     /gene="ruvA"
                     /locus_tag="VXA80_000187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV0352749.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            4703..5266
                     /locus_tag="VXA80_000188"
     CDS             4703..5266
                     /locus_tag="VXA80_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV0352750.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWAQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            5263..5940
                     /locus_tag="VXA80_000189"
     CDS             5263..5940
                     /locus_tag="VXA80_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0352751.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(6077..7108)
                     /locus_tag="VXA80_000190"
     CDS             complement(6077..7108)
                     /locus_tag="VXA80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV0352752.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGRDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(7363..8046)
                     /locus_tag="VXA80_000191"
     CDS             complement(7363..8046)
                     /locus_tag="VXA80_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV0352753.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(8058..9002)
                     /locus_tag="VXA80_000192"
     CDS             complement(8058..9002)
                     /locus_tag="VXA80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0352754.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            9134..11965
                     /gene="uvrA"
                     /locus_tag="VXA80_000193"
     CDS             9134..11965
                     /gene="uvrA"
                     /locus_tag="VXA80_000193"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV0352755.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            11958..13019
                     /locus_tag="VXA80_000194"
     CDS             11958..13019
                     /locus_tag="VXA80_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0352756.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMDLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            13151..13549
                     /gene="spx"
                     /locus_tag="VXA80_000195"
     CDS             13151..13549
                     /gene="spx"
                     /locus_tag="VXA80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV0352757.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            13615..14184
                     /locus_tag="VXA80_000196"
     CDS             13615..14184
                     /locus_tag="VXA80_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0352758.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            14271..14537
                     /locus_tag="VXA80_000197"
     CDS             14271..14537
                     /locus_tag="VXA80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV0352759.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            14541..14960
                     /gene="ruvX"
                     /locus_tag="VXA80_000198"
     CDS             14541..14960
                     /gene="ruvX"
                     /locus_tag="VXA80_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV0352760.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            14976..15281
                     /locus_tag="VXA80_000199"
     CDS             14976..15281
                     /locus_tag="VXA80_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV0352761.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            15589..16839
                     /locus_tag="VXA80_000200"
     CDS             15589..16839
                     /locus_tag="VXA80_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0352762.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKTGKKAVIAKLPPEARLICQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            16836..17393
                     /locus_tag="VXA80_000201"
     CDS             16836..17393
                     /locus_tag="VXA80_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV0352763.1"
                     /translation="MTPLFELVILGKLLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            17539..19071
                     /gene="cls"
                     /locus_tag="VXA80_000202"
     CDS             17539..19071
                     /gene="cls"
                     /locus_tag="VXA80_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV0352764.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTLSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            19329..20867
                     /locus_tag="VXA80_000203"
     CDS             19329..20867
                     /locus_tag="VXA80_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0352765.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQTKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDTIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            20987..23194
                     /gene="nrdD"
                     /locus_tag="VXA80_000204"
     CDS             20987..23194
                     /gene="nrdD"
                     /locus_tag="VXA80_000204"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV0352766.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLDDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            23206..23346
                     /locus_tag="VXA80_000205"
     CDS             23206..23346
                     /locus_tag="VXA80_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352767.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGVGKKERLLSFREQFLNKNK
                     KK"
     gene            23439..23897
                     /locus_tag="VXA80_000206"
     CDS             23439..23897
                     /locus_tag="VXA80_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0352768.1"
                     /translation="MMTEFEKSQSAHDGGFWDTENFVYEEWLETNIQKEMGINLPENR
                     VPSIQFVLFGESGCALRFLNLRLRLNERLLNYAGHIGYSIRPSERGKGYAKETLRQGL
                     QVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVERYWIEVANE"
     gene            23890..24480
                     /gene="nrdG"
                     /locus_tag="VXA80_000207"
     CDS             23890..24480
                     /gene="nrdG"
                     /locus_tag="VXA80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV0352769.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            24488..25105
                     /locus_tag="VXA80_000208"
     CDS             24488..25105
                     /locus_tag="VXA80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV0352770.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            25371..25679
                     /gene="rpsJ"
                     /locus_tag="VXA80_000209"
     CDS             25371..25679
                     /gene="rpsJ"
                     /locus_tag="VXA80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV0352771.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            25896..26522
                     /gene="rplC"
                     /locus_tag="VXA80_000210"
     CDS             25896..26522
                     /gene="rplC"
                     /locus_tag="VXA80_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV0352772.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            26547..27170
                     /gene="rplD"
                     /locus_tag="VXA80_000211"
     CDS             26547..27170
                     /gene="rplD"
                     /locus_tag="VXA80_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV0352773.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVIVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            27170..27466
                     /locus_tag="VXA80_000212"
     CDS             27170..27466
                     /locus_tag="VXA80_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV0352774.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            27484..28317
                     /gene="rplB"
                     /locus_tag="VXA80_000213"
     CDS             27484..28317
                     /gene="rplB"
                     /locus_tag="VXA80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV0352775.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            28421..28702
                     /gene="rpsS"
                     /locus_tag="VXA80_000214"
     CDS             28421..28702
                     /gene="rpsS"
                     /locus_tag="VXA80_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV0352776.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            28714..29058
                     /gene="rplV"
                     /locus_tag="VXA80_000215"
     CDS             28714..29058
                     /gene="rplV"
                     /locus_tag="VXA80_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV0352777.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            29071..29724
                     /gene="rpsC"
                     /locus_tag="VXA80_000216"
     CDS             29071..29724
                     /gene="rpsC"
                     /locus_tag="VXA80_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV0352778.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDVLRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            29728..30141
                     /gene="rplP"
                     /locus_tag="VXA80_000217"
     CDS             29728..30141
                     /gene="rplP"
                     /locus_tag="VXA80_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV0352779.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            30151..30357
                     /locus_tag="VXA80_000218"
     CDS             30151..30357
                     /locus_tag="VXA80_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV0352780.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            30382..30642
                     /gene="rpsQ"
                     /locus_tag="VXA80_000219"
     CDS             30382..30642
                     /gene="rpsQ"
                     /locus_tag="VXA80_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV0352781.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            30668..31036
                     /gene="rplN"
                     /locus_tag="VXA80_000220"
     CDS             30668..31036
                     /gene="rplN"
                     /locus_tag="VXA80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV0352782.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            31114..31419
                     /gene="rplX"
                     /locus_tag="VXA80_000221"
     CDS             31114..31419
                     /gene="rplX"
                     /locus_tag="VXA80_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV0352783.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            31443..31985
                     /gene="rplE"
                     /locus_tag="VXA80_000222"
     CDS             31443..31985
                     /gene="rplE"
                     /locus_tag="VXA80_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV0352784.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            32003..32272
                     /gene="rpsN"
                     /locus_tag="VXA80_000223"
     CDS             32003..32272
                     /gene="rpsN"
                     /locus_tag="VXA80_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV0352785.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            32557..32955
                     /gene="rpsH"
                     /locus_tag="VXA80_000224"
     CDS             32557..32955
                     /gene="rpsH"
                     /locus_tag="VXA80_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV0352786.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGSNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            33147..33683
                     /gene="rplF"
                     /locus_tag="VXA80_000225"
     CDS             33147..33683
                     /gene="rplF"
                     /locus_tag="VXA80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV0352787.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            33767..34123
                     /gene="rplR"
                     /locus_tag="VXA80_000226"
     CDS             33767..34123
                     /gene="rplR"
                     /locus_tag="VXA80_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV0352788.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            34141..34635
                     /gene="rpsE"
                     /locus_tag="VXA80_000227"
     CDS             34141..34635
                     /gene="rpsE"
                     /locus_tag="VXA80_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV0352789.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            34607..34831
                     /gene="rpmD"
                     /locus_tag="VXA80_000228"
     CDS             34607..34831
                     /gene="rpmD"
                     /locus_tag="VXA80_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV0352790.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            34976..35416
                     /gene="rplO"
                     /locus_tag="VXA80_000229"
     CDS             34976..35416
                     /gene="rplO"
                     /locus_tag="VXA80_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV0352791.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            35429..36739
                     /gene="secY"
                     /locus_tag="VXA80_000230"
     CDS             35429..36739
                     /gene="secY"
                     /locus_tag="VXA80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV0352792.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            36890..37528
                     /locus_tag="VXA80_000231"
     CDS             36890..37528
                     /locus_tag="VXA80_000231"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV0352793.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            37645..37863
                     /gene="infA"
                     /locus_tag="VXA80_000232"
     CDS             37645..37863
                     /gene="infA"
                     /locus_tag="VXA80_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV0352794.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            37888..38004
                     /gene="rpmJ"
                     /locus_tag="VXA80_000233"
     CDS             37888..38004
                     /gene="rpmJ"
                     /locus_tag="VXA80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV0352795.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            38022..38387
                     /gene="rpsM"
                     /locus_tag="VXA80_000234"
     CDS             38022..38387
                     /gene="rpsM"
                     /locus_tag="VXA80_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV0352796.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            38405..38788
                     /gene="rpsK"
                     /locus_tag="VXA80_000235"
     CDS             38405..38788
                     /gene="rpsK"
                     /locus_tag="VXA80_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV0352797.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            38831..39766
                     /locus_tag="VXA80_000236"
     CDS             38831..39766
                     /locus_tag="VXA80_000236"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV0352798.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            39778..40164
                     /gene="rplQ"
                     /locus_tag="VXA80_000237"
     CDS             39778..40164
                     /gene="rplQ"
                     /locus_tag="VXA80_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV0352799.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            40429..40695
                     /locus_tag="VXA80_000238"
     CDS             40429..40695
                     /locus_tag="VXA80_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV0352800.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYFRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            40705..42042
                     /locus_tag="VXA80_000239"
     CDS             40705..42042
                     /locus_tag="VXA80_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV0352801.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            42286..42978
                     /locus_tag="VXA80_000240"
     CDS             42286..42978
                     /locus_tag="VXA80_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0352802.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(43081..44733)
                     /locus_tag="VXA80_000241"
                     /pseudo
     CDS             complement(43081..44733)
                     /locus_tag="VXA80_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(44783..45814)
                     /locus_tag="VXA80_000242"
                     /pseudo
     CDS             complement(44783..45814)
                     /locus_tag="VXA80_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(45827..46276)
                     /locus_tag="VXA80_000243"
     CDS             complement(45827..46276)
                     /locus_tag="VXA80_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0352803.1"
                     /translation="MCTILVSIHYKIGLSLSGVYKAVVDGEMAVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKAKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            complement(46352..46948)
                     /locus_tag="VXA80_000244"
                     /pseudo
     CDS             complement(46352..46948)
                     /locus_tag="VXA80_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(47165..47362)
                     /locus_tag="VXA80_000245"
     CDS             complement(47165..47362)
                     /locus_tag="VXA80_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0352804.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(47437..>47683)
                     /locus_tag="VXA80_000246"
     CDS             complement(47437..>47683)
                     /locus_tag="VXA80_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV0352805.1"
                     /translation="VLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENK
                     YRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
BASE COUNT        14475 a         8908 c        10348 g        13952 t
ORIGIN      
        1 gcataaaatc aggtgttcaa aaaccttgat tttatgcgtt ttatcataga aatatttatt
       61 tcattttttc ctaattcttt tcgaaactct ttttaaacga cgtcagtttt atcagtaatc
      121 tcaaaacagt gttttgagct aattttgcca gttttgtctg taacatcgaa gttgtgtttt
      181 accactctgc gactggtttc ctagtttgct ctatgatttt cacagagtat taaattgcga
      241 ttttgccaag tttctttatt cgtctaaaag tagagtctgt tctatgcgtc taatgtacga
      301 attaggttga ccatttcaat agctccttgt gcacactcag aacccttatt tcctgcttta
      361 gtaccagctc gttctatggc ttgttcaatt gtatctgtcg ttagcacacc aaacataaca
      421 ggaatttcgc tatttaaact gatttgggcg attcccttag atacctcgct acatacataa
      481 tcataatgac ttgtattccc tctaatgaca gctcccaagc agataattgc atcatatttt
      541 ttactttttg ccatttttga tgcaatcagt gatatttcaa aagctcctgg aacccaggct
      601 acctctatat ctttctcgtt tacattctct cttttgagat tatctagtgc tccagataat
      661 aattttgaag ttataaattc attaaatctc gctacaacaa tacctatttt aatattgttt
      721 gctactaaat taccttcata agtgttcatt tatttttcct ccatatttaa aatgtgaccc
      781 attcgatttt tctttgtttc taaataaaaa ctatcgtaag gattggcttc tatttcgatt
      841 gatattctac tggaaatggt aattccatat ttttctaact gttcaacctt gtcaggatta
      901 tttgtcagta aatgaagtga ctgaagtccc agatctttaa gcatttgtgc tccaatatga
      961 tattctctta aatcaccttc aaagcctaat gcaagattgg catcaagcgt atccatgcct
     1021 tgatcttgta aatgataggc ttttaattta ttgataagtc caattcctcg tccctcctgt
     1081 cgcaagtaaa gtaagacacc cgaaccattc tcaacaatca ttttcatagc tttatcgaat
     1141 tgctgtccac aatcgcaacg taaggagcct aaaacatccc ctgttaaaca ttcggagtgg
     1201 acccgacata atacattggc ttcatcctct atgtttccca taataagagc aagatgatgt
     1261 tccccattta gtttatctat atagctaatt gctttgaaat taccgtatct agtaggcata
     1321 ttgacagttg aaactcgttc taccagctga tcatatactt ttctatattc ttgtaattct
     1381 ttgatggtaa ttagtggaat gttgtgtttc ttcgagaact gaattaaatc atctgttctc
     1441 atcattttgc catcatgatt cattatttca caacataggc cacactcttt tagtccagct
     1501 aattttaata aatcaacagt tgcttctgtg tgtccacttc tttctagaac accacctttt
     1561 tttgcaatta aaggaaacat gtgtcctggc ctgcgaaaat cagagggtgt tatatcttca
     1621 gctacacaca tacgtgcggt cagtcctctt tcctcggcag aaatacctgt ggtcgtttct
     1681 ttataatcaa ttgaaactgt aaaagcagtc ttatgattat ctgtattgtt ttcaaccata
     1741 ggtgaaagca ttaattgatt agctaaactt tcgctcatag gcatacaaat taatcctttg
     1801 gcataagtag ccataaaatt aacattttct gttgtagctg cttgtgcaga acaaattaag
     1861 tctccttcat tttctctatc cttgtcgtct ataacaagaa caagtcgtcc cttctgcaat
     1921 gcttctaatg cttcttgtat ttttcgatat tccattgact gattatcctt tctgctaaaa
     1981 tccattttga tataatagtt ccttagatat ttctgatttt ggagagttat ccatcagttt
     2041 ttgcacatat ttacctaaga tatcattttc aagatttact gtactcccga cttgtttact
     2101 cttaagaatg gtttgttcca aggtatgagg gataacagat actgaaaagt ttactttgga
     2161 gactttagtg acagtcagac taatgccgtc aattgtaata gatccttttt caactattaa
     2221 atctaaaatt tctttttgtg tgttgatttg ataccataca gcattatcat ctttttttat
     2281 tgacgagatt tttcctgtac catcaatgtg tcctgtaacg acgtgacccc caagtcgacc
     2341 gttgacagat aaggctcttt ctagattcac ctcacttcca tgttttaata gagtaagagc
     2401 tgttcgactc catgtttcat tcattacatc aactgtaaag gattgatgat tgaaatgagt
     2461 aactgtaaga cagataccat ttactgctat actatcgcct aaatggatat ccgttaatat
     2521 ttttgaggct ttaattgata gtttacaatt acgagagtct ttctgtattc tttcaacttt
     2581 tccgatttct tcaattattc ctgtgaacat ggataaatca cttcactttc tatgagatag
     2641 tcatttccta tttgagaaaa tgcataaggt ttcaatctaa tagcgtcatt tggcaaagaa
     2701 ataccttcac ctccgacagg aaacttggca ctacctccaa aaatttttgg tgcaatatat
     2761 attttcagct catcaacaat ttgttgttcc aaagcactcc aattcattag actgccccct
     2821 tctagaacta ggctatcaat ctgcatgttt cctagatgtt gcattaaact cgataagtct
     2881 atatgattgc cttttttctt tatggaaagt atttcacagc catgattttg atatagcttc
     2941 attttatttt tgtcttcaga ggaagtggca atgtaagttt taatatcatt tgctgttttt
     3001 acgattttag aggtaagagg agttcgtaaa tgtgtatcgc atatgatacg gataggattt
     3061 ttcccttcct ccaatctaca tgtcagcaaa ggatcgtctt gaataacagt attgactccc
     3121 accataattg cactaacatg gtgtcgtaac tgatgcacat gctttcttgc ttcttcttca
     3181 gtaatccatt tggattgatt tgttttagtg gctatttttc catccattga cattgcatat
     3241 ttcataaaaa catagggtac atgctgggta atatactttc taaaactttt tattaagtta
     3301 agacactcat tttctaaaat tccaacagta acttgaagat tattttcctc aagtatcttt
     3361 actccttttc cagatacaat aggattacag tctaggcttc caatgactac tcttgtaata
     3421 ccactatcga ttatagcatc tatacaggga ggtgttttcc cgaagtgaca acagggttca
     3481 agtgttacat aaagcgtcgc tccgacaggg gattctctac agtttttaag agcatttctc
     3541 tcagcatgtg ggccaccaaa aaactcatga taaccttgtc cgataatgtg attatctttt
     3601 acaataactg cgccgaccat aggattggga ttgacgtaac cagccccttt ttgtgccagt
     3661 tttattgcta atttcatata ttttgaatcg ctcatctcgc tacctccaaa aaaatatacc
     3721 ttgaataggg gactactcaa ggcatacaaa agaaaactta tgcgattaac aaaaatgctc
     3781 tgaaatgaca agtaatcatt tcagagcacg caaaaagcac aaatatactt ttatcttctt
     3841 tcatccagac tatactgtcg gctttggaat ttcaccaaat catgcctttc ggctcgtggg
     3901 ctataccacc ggtagggaat ttcaccctgc cctgaagata gttattcaat tacagatgat
     3961 tatagtactt aattttgaat atgtcaacag ataaataccg attgtttttg atatactgta
     4021 tttgtgataa tcgattctcg ctcctcggat aaagaaaata tgatatacta gataaacgaa
     4081 ataagagaga aggaatacta tgtacgcata tttaaaagga atcattacca aaattactgc
     4141 caaatacatt gttcttgaaa ccaatggtat tggttatatc ctgcatgtgg ccaatcctta
     4201 tgcctattca ggtcaggtta atcaggaggc tcagatttat gtgcatcagg ttgtgcgtga
     4261 ggacgcccat ttgctttatg gatttcgctc agaggatgag aaaaagctct ttcttagtct
     4321 gatttcggtc tctgggattg gtcctgtatc agctcttgct attatcgctg ctgatgacaa
     4381 tgctggcttg gttcaagcca ttgaaaccaa gaacatcacc tacttgacca agttccctaa
     4441 aattggcaag aaaacagccc agcagatggt gctggacttg gaaggcaagg tagtagttgc
     4501 aggagatggc cttcctgcca aggtcgcagt gcaagcaagt gctgaaaacc aagaattgga
     4561 agaagctatg gaagccatgt tggctctggg ctacaaggca acagagctca agaaaatcaa
     4621 gaaattcttt gaaggaacga cagatacagc tgagaactat atcaagtcgg cccttaaaat
     4681 gttggtcaaa taggagcaga gaatgacaaa acgttgttcg tgggtcaaga tgaccaaccc
     4741 actctacatc gcctatcatg atgaggagtg ggcccagccc ctccatgatg accaagcgtt
     4801 gtttgagttg ttgtgtatgg aaacctatca ggcaggcctg tcttgggaaa cggtactcaa
     4861 caaacgccaa gctttccgag aagcctttca tagctatcaa attcactcag tcgcagagat
     4921 gactgacact gaattggaag ccatgctgga gaatccagct atcattcgaa atagagccaa
     4981 gatttttgct acacgcgcta acgcccaagc ctttctacag ttacaggcag agtacggctc
     5041 ttttgatgcc tatctttggt cttttgttga ggggaaaact gtcgttaacg atgttcctga
     5101 ttatcgccaa gccccagcta aaacaccctt atctgagaaa ttagccaaag atctcaaaaa
     5161 acgaggcttc aagttcacag gcccagtcgc cgtattgtct tttctacagg ctgcagggct
     5221 agttgatgac cacgagaatg attgtgagtg gaaaggtctt aaatgatgtc taacaaaaat
     5281 aagggaattc tgatttttgc gattctctat acagtcctct ttgtgtttga tggcgttaaa
     5341 ttgctggctt ctttaatgcc atctgccatt gcaaattatc ttgtttatgt agttttagct
     5401 ttatatggct ccttcttgtt caaggataga ttgatccaac aatggaagga gattagaaag
     5461 actaaaagaa aattcttctt tggagtctta acaggatggc tctttctcat tctgatgact
     5521 gttgtctttg aatttgtatc agagatgttg aagcagtttg tgggactaga tggacaaggt
     5581 ctaaatcagt ctaatattca aagtaccttt caagaacaac cactactgat agctgttttt
     5641 gcttgtgtca ttggacctct ggtagaagaa ttatttttcc gtcaggtctt attgcattac
     5701 ttgcaggaac ggttgccagg tttactaagc attattctgg taggacttgt ttttgctctg
     5761 actcatatgc acagtttggc tctatcagag tggattggtg cagttggtta cttaggtgga
     5821 ggccttgcct tttctattat ttatgtgaaa gaaaaagaga atatctacta tcccctactt
     5881 gttcacatgt taagcaacag cctctcctta atcattttag ctatcagtat agtaaaatga
     5941 aatgagaaca ggacaaatcg atttctaaca atgttttaga agtagaggtg tactattcta
     6001 gtttcaatat actgtaatat gtgatgaaaa tgccagtaat gataccgaga aaaaagctga
     6061 gaaacttttc ccagctttat ttgttatagt caaagagaat gacttgttcc tgtgcatcta
     6121 catgagcatg gaccccaaag ggtacaattg ctcttggagt tgcgtggccg acattcagat
     6181 tatagacaat cgggatattg ctgtcaatga tatccaatag tgcctcttta tagtcgtcat
     6241 ggaaagtttc atccataggt tttccgacca agagtccatt gatgaccgcg aatatgccag
     6301 tgtcctttaa agttagcaac atctttttga agtcttctgg cttaggcttt tcttcgcttg
     6361 tttcgagcaa gaggattttc ccttcccagt ctgacaagtc agggaaaagt ttgtattttt
     6421 ggcagagttc cgtgctatct gcgtatcgag agttgtcaaa gatatcgtag agggattcga
     6481 ggcaaccacc gaggattttc ccctcgaact gggcacttcc ttgcaacaag tcaaaacctg
     6541 tatttgtatg actgacacga ggtgttccca gggccgtggg actaaaatca gttcgttcct
     6601 cataccaaac gtcactaggg cggatttctg aaattcttcc cgtctcaatc aattctttaa
     6661 agtagtgaag gctataggct agcatttctt tgtctaattc acaaatgtct gctaaaaagg
     6721 attgaccata aaaagtcttg attcctaatt tatgcaacat gaggtggttc atggttgtat
     6781 ccgagaagcc aagaaaaatt ttttgtttga taaccttttg gagttggtca ttttcaaaaa
     6841 gataaggtag caagcgatag gtatcgtctc taccgatggc acataggatc atgtcgatgc
     6901 tatcatcaga aaaggcatga atcaaatcct ctgcacgagc ttcaggatgg tccttgataa
     6961 agtctaatcc ttttaacgaa tggggcaaaa agatgggatt gagtcccaga tccttgagac
     7021 gttggatacc caagtccact tcgtgtttga caaaatcctc tccgataatg ccactagata
     7081 aactaacaat accaatagta gaaaccatat ctcatcctcc tagaaataga ttgagcctat
     7141 tttatcacaa aatataagag aaagctatgt gggatttcag aagaatactt ttaatactct
     7201 tcgaaaatct cttcaaacca cgtcagtttt atttgcaacc tcaaagctgt gctttgagca
     7261 acctgcagct agcttcctag tttgctcttt gattttcatt gagtataact caaaaaagta
     7321 agtgaaaaag caaccgcacc tgcagttgct tttatcgttt tcttattctt gatagcgtgt
     7381 tggtcctgca cttgcactac ggatattcaa gcgtttcttg agatagaagc gaagggctag
     7441 gagggctact ccaataatag ctagtggcaa tggatccagt actgggttaa ggctagctgg
     7501 taggaagctt gttgcaaaga agacaagcaa ccaaaggaac atagaagcta ggataactag
     7561 tacagatttc cagaaaggtg gacgttgact gcgatccata tctggtccat agtattggta
     7621 aacaaagtag tacatcaagt agaaggcaaa tccaccaacc agtccaacta atagaagagt
     7681 aatcaatcca tagccgaaag cttgatctgc cgcaaagaag gttgtgaggg cgctgacaag
     7741 ggcaaagagg ctagtgatga aaagagctga gtccataatc atcagttttg ggtcatcatt
     7801 ttcttttgga tgctcttttt cgtactgctc tttgacagtg aagctatgag cccaatgagt
     7861 tggtgcgccg tatagggaac gggcagttgt acctttagat tgctcctcaa ggatttgggg
     7921 aatgacttcc tcaaaaatag tctggatttc agcgtctgtt ttcccatctt tgatgaattg
     7981 ttgggtagca atgtggacaa actcttggtt tttcttagtt aatttttgta gatcaatttg
     8041 agacatagga actcctctta gaaccatttt ttatggatga gatagagagt gagcgagaca
     8101 ctcatagcaa aggcgataaa gacgattaac cagaaggcat ttggctctcc gtttagggga
     8161 atttcattat ccttaaagtt catcccgtag gcagaaaaga ccatggttgg gatggacatg
     8221 acgatggtca caagggccaa ggttttcatg atgttgttct ggttgttaga aatgatagag
     8281 gcaaaggtct ctgtcataga atgcaagacg tttccataaa tatctgccat ctcgatggcc
     8341 tgttgggttt caatcagggt gtcttcaagc aggtcttcgt cctcaaggta tttcttgata
     8401 ttgctggttg aactggtcaa tttcttaatc acgcgctcat ttgttttgag ggaggccttg
     8461 aaatagacga tagttttttc caattccatg agctcaatca attcttcatt acgagttgat
     8521 tgatgcagtt gactttcgat ttgttcactc ttgcggtcga ttgaacgaag ggctgttagg
     8581 taaagctctg cattgcgata aagaatttga aagataaaac gtgaacgcat gaaggtatag
     8641 aaattacgca atcgacggtt gataaagaca tcaaggacag gtagtggttc caaacacgta
     8701 gtgataatgg tttcctcagt gatgataata ccaagcggga tggttacgta gtaggtgcgg
     8761 ttatttcttt cctccgtgac cggcacgtct acgataatca gggtatactc gtcttcaatg
     8821 gtaatacgag acatttcttc cgcatcgagc ggtgctcgaa ggtcggcaat atcaatatcg
     8881 aaggtgttgg cgatttcgag tgattcattt tgagtcggat tgacgagatt gatccaagta
     8941 cccggttcaa gcgtatcgat ctctttaaat tcagttgttg tagagagaaa aacttgtttc
     9001 atatccctta tcctttctca tttttcagat tttttcacac tgtactatta tactacaaaa
     9061 tcggcctttt gggtaataga atctcacttt ttttggtata atggtaagca ataatggact
     9121 agaaagaaca aagatgcaag ataaaattgt cattcatggg gcgcgtgccc ataatttaaa
     9181 aaatattgat gtggagattc cgcgagacaa gttggttgtc gtgactggct tgtcaggttc
     9241 agggaagtcc agtctggctt ttgatactct ctatgcggag ggacaacgtc gctatgtaga
     9301 gagtttgtct gcctacgctc gtcagttctt gggaaatatg gaaaaaccag atgtagatgc
     9361 cattgatggt ctcagcccag ctatttccat cgaccagaaa acgactagta aaaatcctcg
     9421 ctcgacggtg ggaacgacga ctgaaatcaa tgactatctg cgtctcctct acgcacgtgt
     9481 ggggacgcct tactgtatca acggacatgg agctatcaat gcttcttctg tggagcaaat
     9541 cgttgataag gttttggagt tacctgaacg ccagcgcttg cagatcttgg ctcctgtcat
     9601 ccgcaagaaa aaaggccaac ataagagtgt tatcgagaag gttcagaaag acgggtatgt
     9661 tcgtgttcgt gtggatgggg aagtctatga tgtgaccgaa gtgccagagt tgtctaagag
     9721 caagcaacac aatatcgatg tcgtggttga ccgtattgtt atcaaggagg gcattcgtag
     9781 ccgtctcttt gattccattg aagctgctct tcgtatcgca gaaggttatg tgattatcga
     9841 caccatggac gactctgagt tgttgttctc tgagcattat gcctgtccag tttgtggatt
     9901 tactgtccca gagttagaac ctcgtctctt ctctttcaat gctccctttg gctcttgtag
     9961 tgagtgtgac ggcttgggga ttaagctgga ggtggatact gatttggtag tgccagatgc
    10021 cagcaaaacc ttacatgagg gagcgctggc accttggaat cctatctcat ccaactacta
    10081 tccaaacatg ttagagcagg ccatgaaagt ctttggagtg gctatggata agccttttga
    10141 ggacctgtca gaagaagata agaacttgat tctctacggc tcagatggta aggaattcca
    10201 tttccattat gaaaatgaat ttggtggtgt gcgcgatatc gacattccgt ttgagggagt
    10261 tatcaataat atcaaacgtc gttaccatga aaccaatagc gattacactc gcactcagat
    10321 gcgtctctac atgaatgagc tgacctgcgg aacctgtcaa ggctatcgtc tcaatgacca
    10381 ggccttgtct gtccgtgtgg gcggccagca agggccacat attggagaaa tctcagacct
    10441 gtctatcgct gaccacttgg acttggtgag ccagttaact ttgtctgaaa atgaagccat
    10501 cattgctcgt cccattctca aggaaatcaa ggatcgtttg accttcctta ataatgtggg
    10561 tcttaactat ctaaccctgt cacgttcagc aggaaccctt tcaggtgggg aaagtcagcg
    10621 cattcgtttg gcaacccaga ttggttccaa cctatcaggt gtcctctata tcctagacga
    10681 gccgtcaatc ggtcttcacc agagggacaa tgaccgcctg attgccagtc tgaaaaagat
    10741 gcgtgacttg ggcaatactc ttatcgtggt ggaacacgac gaagatacca tgcgtgaagc
    10801 tgattatctg attgacgttg gtcctggtgc cggtgttttt ggtggtgaga ttgttgcagc
    10861 aggtacacct aagcaagtgg ctcgcaacag caagtctatc acaggtcagt acttgtcagg
    10921 caaacgtgtc atcccagtac cagaagagcg ccgtgtcgga aatggtcgtt ttattgaagt
    10981 gataggagcg cgtgagaaca acttgcaaaa tgtcacggct cgctttccac taggaaaatt
    11041 tatcgcagtg acaggtgtgt cgggttcagg gaaatcgact ctaatcaaca gcattctcaa
    11101 aaaagccatt gcccagaagc tcaaccgtaa ttcagacaaa cctggtaagt ttaagactat
    11161 tacagggatt gagcatgtcg accgtttgat tgatattgac cagagcccta tcggacggac
    11221 gccgaggtct aacccagcta cctatacagg agtttttgac gatatacgtg acctttttgc
    11281 tcagacaaat gaagccaaga ttcgaggcta caagaagggg cgcttcagtt tcaacgtcaa
    11341 gggaggtcgc tgtgaagctt gctcaggtga cgggattatc aagattgaga tgcacttctt
    11401 gccagatgtt tatgtggctt gtgaagtttg ccacggaacc cgctataaca gtgaaaccct
    11461 agaagttcac tacaaggaaa agaatatttc gcaggtcttg gatatgacgg tcaacgatgc
    11521 ggtagaattt ttccaacaca ttccgaaaat tcaacgcaaa ctccagacca tcaaggatgt
    11581 agggctaggc tatgtgacct tgggacagcc agctaccacc ctttctgggg gagaagccca
    11641 acgtatgaag ttagctagcg aactccacaa acgctcgaca ggaaaatctt tctacattct
    11701 ggatgagccg acgacagggc ttcatacaga ggacattgct cgcctgctca aggttttagc
    11761 tcgctttgta gacgatggca atacagtcct cgtcatcgag cacaatctgg atgttatcaa
    11821 gacggcagac catatcattg acttgggacc tgagggtggt gtcggtggtg gaaccatcat
    11881 cgtaacagga actccagaag aagtagctgc caacgaagcc agctatacag gacattattt
    11941 gaaaggaaag ttacatcatg aataaacgcg tacaagcatt tctagctaaa atgcaagaaa
    12001 aagaactaga tggtatcatc atcaacaatc ttaaaaacgt ctattatttg actggttttt
    12061 ggggctcaaa cggaacagtc tttatcagcc gtgaccgtca ggtcttagtg acagactctc
    12121 gctatatcat cgcagctaag caagaaacca gtggttttga gattgtggct gatcgtgatg
    12181 aattggctgt cattgcagga attgttaagg acatggactt gactcgtatc ggttttgaag
    12241 atgagatttc agtgtcttat taccaccgta tgcaggcagc ttttgcaggt ttggacttgc
    12301 ttccacaaac tcagtttgtg gaaggtcttc gtatgattaa ggatgaggca gagattgcag
    12361 cgattcgcaa ggcttgttct atctcagacc aagctttccg cgatgcgctt gactttatca
    12421 aaccaggaaa aactgaaatt gagattgcca acttccttga tttccgcatg cgtgagttgg
    12481 gagcatctgg cttatctttt gatacgatcc tagctagcgg tatcaattct tctaaacccc
    12541 atgcccatcc aatgcacaaa ccagtggagt tgggagaagc cattaccatg gacttcggct
    12601 gtctctatga ccactatgtc agtgatatga cccggactat ctatctaggg catgttagcg
    12661 atgagcaggc agagatttac aatacggttc taaaagctaa ccaagccttg attgaccaag
    12721 ctaaggcagg cttaggtttc cgtgactttg acaaaatccc tcgtgatatt atcattgagg
    12781 caggttatgg cgactacttt acccacggca ttggccacgg tattggtctg gatatccatg
    12841 aggaacccta ctttagccag acttctacag aaactattaa gacaggtatg gccttgaccg
    12901 atgaaccagg tatctatatc gaaggcaaat atggcgttcg tatcgaggat gatatcctga
    12961 ttacagagac aggttgtgaa ttattgaccc tagctccaaa agagttgata gtcatttagt
    13021 tgtttgtcaa gaaaaaagta cgaaaatgac gaaaaaagtc aaaaaaaatt aaaaataggt
    13081 cgcaagtcgg atgtttttta tggtataata gactaaactg atagtaacat gtagcgaaag
    13141 gggtaggtac atgattaaaa tttatacagt ctcaagttgt actagctgta aaaaagcaaa
    13201 aacctggctc aatgcccacc agttaagtta taaagaacaa aaccttggta aagaaggaat
    13261 tacgagagaa gaattactgg atattctaac caaaacagat aacggaatag ccagcatcgt
    13321 ttcatctaaa aatcgctatg ccaaagccct tggagtggat attgaagatt tgagtgtcaa
    13381 tgaagttctc aatctgatta tggaaacacc gagaatttta aagagcccaa tccttgtaga
    13441 tgaaaaacgc ctgcaagttg gctacaagga agacgatatt cgtgccttcc taccacgctc
    13501 tgtccgtaat gtagaaaatg cagaagcacg tttgcgtgca gctctataaa catcaaggct
    13561 gggagcactt cccagtctta ttctatttta atttcaaaaa gaaagaagaa agaaatgaaa
    13621 aaaatagttc ttgttagtct agctttcctt tttgtcctgg ttggttgcgg acagaaaaaa
    13681 gaaactggac cagctacaaa aacagaaaaa gatacgcttc agtcggcatt gccagttatt
    13741 gaaaatgctg agaagaatac agttgtaact aagactttgg tcttgcccaa gtcagatgat
    13801 ggtagccagc agacacaaac aattacttat aaagacaaga cttttttgag tctaactatc
    13861 caacaaaaac gtccagtctc tgatgagttg aagacttata ttgaccaaca tggagtggag
    13921 gaaactcaaa aagctcttct tgaagcggag gagaaggata agtctatcat tgaagctcgt
    13981 aaattggcag gtttcaaact tgaaacaaaa ctattgagcg caacggaact tcaaacaacg
    14041 actagttttg attttcaagt tctggatgtc aagaaggctt cccagttgga acatctgaag
    14101 aatattggtt tggaaaatct tttgaaaaat gaaccaagca aatatatttc agatagattg
    14161 gcaaatggcg cgacagaaca atagaaaatc caaataaata gactgcctga attgtagaag
    14221 gtaaggaatt atgctataat ggtactattc taaggaaaga gggtgttaga atgggattta
    14281 ctgaagaaac agtacgtttt aaattggacg attccaataa aaaagaaatt agcgaaactt
    14341 tgacagatgt ttatgcttcg ttgaacgata agggttacaa cccaattaac caaatcgtag
    14401 gttacgtatt gagtggagac cctgcctacg ttcctcgtta taataatgca cgaaatcaaa
    14461 tccgtaagta tgagcgtgat gaaatcgttg aggaattggt tcgctactac ctcaaaggac
    14521 aaggagtcga tctataacct atgagaatta tgggattgga cgtcggttca aaaacggtag
    14581 gggtggcgat tagcgatccg cttggtttta cagctcaagg gcttgaaatc atccagataa
    14641 atgaagaaca aggccaattt ggttttgacc gcgttaagga attggttgat acttacaagg
    14701 tggaacgatt tgtagtgggc ttgcctaaaa acatgaacaa tacaagtgga ccgcgcgtag
    14761 aagctagtca agcctacgga gcaaagctag aagagttttt tggtttacca gtagactatc
    14821 aggatgaacg cttgacaaca gtggctgctg agcgcatgtt gattgaacaa gcagatatca
    14881 gtcgcaataa gcgcaagaaa gtcattgata agttagcagc tcagctgatt ttacaaaatt
    14941 atttagatag aaaattttaa tataaaggag aggctatgtc acacgatcat aaccacgacc
    15001 acgaagaacg tgaactaatc acactagtag atgagcaagg aaatgaaacc ttgtttgaaa
    15061 tccttttgac aattgatgga aaagaagaat ttggtaaaaa ctatgttctt ctagtaccag
    15121 ttaacgcaga agaagacgaa gacggacaag ttgaaatcca agcttactca ttcattgaaa
    15181 atgaagatgg aacagaaggc gaattgcaac caatcccaga agactcagaa gacgaatgga
    15241 acatgattga agaagtcttc aacagcttta tggaggagta aaaacgtccg ggggacgttt
    15301 ttagccctgc gctagaaatt agaaacgcag gtagtctggg agactgtatc agcccaactc
    15361 ccagaaatgg ggaagagttg gaatgtccag tggatgtttt tagcccagtt cctttaaata
    15421 agatagaact ggtaggttcg gggggacgtt tttagcccat attcacattc atagtagcta
    15481 gttattagct aaccaggtaa gaggtcggga cgaaaatcct ggccttgttt ttattagtaa
    15541 ccatacttta atgcgataat tgattggact tttgtcaagg agatgtgtat gtttgaagta
    15601 gaagaatggc tccatagtcg gattggtttg aattttcgat caggtttggg ccgaatacag
    15661 caagcggtgg atttgttagg aaatcctgag cagtcttacc ctattatcca cgtaacaggg
    15721 actaatggga aaggatctac cattgctttt atgagggaat tatttatggg gcatggcaaa
    15781 aaagttgcga cctttacctc ccctcatatc gtctctatca atgaccgaat ctgcattaat
    15841 gggcaaccta tagcagatgc agactttatc cgtttggctg atcaggtcaa ggagatggag
    15901 aaaacgcttc tgcaaactcc tgaccagttg tccttttttg aattgctgac cttggttgct
    15961 tttctttatt ttagggagca agaggtggat ttggttttat tagaagtggg aattggtggc
    16021 ttacttgaca cgaccaatgt ggtaactgga gagcttgctg tcatcacctc cattgggctt
    16081 gatcatcaag aaaccttggg tgatagtcta gaagcaattg cagagcagaa agctggtatt
    16141 ttcaagactg gtaagaaggc agtgattgca aaattgcctc cagaagctag gcttatctgt
    16201 cagaaaaaag ccgaatcttt agctgttaac ctttatcagg caggtcaaga ttttttaatg
    16261 ctgaatggtg atttttcaag ctctttacta aatctttcac agctgaacat aggcttagaa
    16321 ggagtctatc agcaggagaa tgcagccttg gcgttgcaaa cttttcttct ttttatggga
    16381 gaaggaaagg aagctgttga tgaacaggct gtaagaaagg ccttggaaca gacccattgg
    16441 gctggtcgct tggagcgtat tcgcccacag atttacttgg atggtgctca taacctccct
    16501 gccttgactc gcttggttga gtttatcaaa gaaaaagagc aggaaggtta tcgtcctcaa
    16561 atcctctttg gatccttgaa acggaaggat tatcaaggga tgttgggtta tctgactgaa
    16621 aatttacctc aagtggaact caagatgact ggctttgact atcaagggtc tttggacgaa
    16681 agggatgtga caggttacga tatagtttct tcttaccgag aatttatcag cgattttgaa
    16741 gaaagagcag atgctcagga tttgctgttc gttacagggt ctctctattt tatctcagaa
    16801 gtacggggct acctgctgga ccgtgagcag ataaattgac ccccttgttt gaacttgtta
    16861 tactagggaa attgctggtt agagaaaatt tctctaaatt ggtagcagaa aggaaattca
    16921 tcatgaaatt aaaaagattc acactttctc ttgcttctct agcaagtttt agtctcttag
    16981 tagcttgttc acaaagagct caacaggttc aacagcctgt tgctcagcag caggtccaac
    17041 agcctgctca acaacctgct caacagaata ccaatactgc aaatgcagga ggtaaccaaa
    17101 atcaagcggc tccagtacaa aaccaacctg ttgctcaacc gaccgatatt gatgggactt
    17161 atactggtca ggatgacgga gaccgtatca ctttagtggt aactggaacg actggtacat
    17221 ggactgagct cgaatctgac ggggatcaga aagtcaaaca ggttacattt gattcagcaa
    17281 atcaacgcat gattattggc gatgatgtca aaatttacac tgtaaacggt aatcaaatcg
    17341 tcgtagatga tatggataga gacccatcgg accaaatcgt tttaactaaa taagactatg
    17401 caagtagaaa gggctggatt tttcagcctt tttactttta cctagaaaat aatcataaat
    17461 acttgccttc taccgaatac ctgcgttata ctagtatcaa ttacttgttt ttagcattca
    17521 aactatcaag gaggggatat gaaatatagg aaatttcaat tattgatgtc caagtatggc
    17581 tttagtcttt cgattatgct acttgaactt tgtcttgttt ttggtctctt tctttattta
    17641 ggacgcatgg ctcccatttt atggattact gtcctcattc tactgagtat catcacaatc
    17701 atttcgatag tcaaccgtaa tacgactcct gagaataagg taacctggtt gttagtagcc
    17761 tttgtgccag tatttggtcc cttgctctat ctgatgtttg gtgaaaggcg attgtccaaa
    17821 aaagaaatca aacaactgaa gaagctaggc tctatgcatt tccaagaagc aaatagccag
    17881 ctactaaaag agaaattaaa agaaagtgac aaggcagctt atggagtcat caagtcctta
    17941 ttgagtatgg ataccaatgc tgacatctat gatcaaactg cctctacatt ttttcctaac
    18001 ggagaagcta tgtggaaaaa gatggtagaa gatcttaaaa aggctgagaa atttattttc
    18061 ttggaatatt acattataga agaaggtttg atgtggaatc gcatactaga tatactagag
    18121 caaaaggtag ctcagggtgt agaggttaag atgctctatg atgatatcgg ctgtatggct
    18181 actttaacag gagattatgc acatcgactt cgtcagctgg gcatcgaggc ccataaattc
    18241 aataaagtta ttcctcgttt gacagtggct tataataaca gagatcatag aaaaatattg
    18301 attgttgatg gtcagatagc ctatactggt ggggtcaatc tggcagatga gtacattaac
    18361 cacgtcgaga gatttggtta ttggaaggat agtggaattc gcttagacgg actagcagta
    18421 aaagctctga cacgcttatt tttgaccact tggtacatta atcgaggaga aattagtgat
    18481 tttgatcaat atcatttaga aaatcattct atcccgagtg acggtttaac cattccatac
    18541 ggaagtggac ccaagccaat ttttcgagcg caggtaggga aaaaagttta tcagagttta
    18601 atcaatcaag caacagaatc ggtctatatt acgacacctt atttgattat agattatgat
    18661 ttaacagaga caatcaaaaa tgcagctatg agaggggtcg atgttcgaat tatcacccct
    18721 tacataccag ataagaagtt cattcagtta gtcacgagag gagcttatcc cgaccttctt
    18781 tctgctggtg ttcggattta tgagtatagt ccaggtttta ttcatagtaa gcagatgttg
    18841 gtagacgaag attttgcggt ggtggggaca atcaatctcg actaccggag cttggtacac
    18901 cattatgaaa atgcagtctt actctataaa actctttcta taagggaaat cgcccgagat
    18961 tttcgaaata tatttgcaga ttctcaggaa gtctatcctc attctatcaa aacgagctgg
    19021 tatcaaaagc ttgtaaaaga aatcgcccag ctattcgccc ctatcttata agaaatctag
    19081 gacttaggat acaaaccaga cctatttttc ttgtctttta cgaatagaaa gaaagaggag
    19141 gggaattcat gctaactaag gaagaagtga atgatatgat tgagttcaaa cagacacatt
    19201 tacaccgctt tcgggaaatt gaaacctttg tgaaactatg caaaaaatca ctcaaacagc
    19261 catcgcaagc tgacaatcct aggacttttt gatatactaa gacagataaa ttgaacagga
    19321 gaaacgatat gagtgtgtat ggtagagtag aagaagttca taaggagaat cgtgaacctc
    19381 tagaatacca aatcgaacaa gaatcgcatc atcgtgaatc aagtcgtctt cctttggtga
    19441 aaattttact atggagtacg cttgtaacgg ggataactct aggagtaccg ctattactcg
    19501 acttaatgag tgcacaagaa gtgcaggatt tttatgcagg ttgggcgctt catcagacag
    19561 ggaagattta cagcgactat tatggaagtc aaggtttgct ttattatttg ctgacttacg
    19621 tgagtcaggg cggatttttc tttgccatct ttgagtggtt agccttggta gcaggaggat
    19681 ttttcctttt tagatcagcg gacaccttga cagagcaagg agaccaagct ggacatctgg
    19741 tgactatttt ttacatgcta gttacaggtc ttgcttttgg tggaggctat gcgactcttt
    19801 tagcgcttcc tttcttattc gcagccttta gtttagttgc ggcttaccta agcaatccaa
    19861 gccatgataa gggatttgta cggattgggc tagctttggc aggcggattt ttctttgctc
    19921 ccttatcatc gctcctgttt attgctgtag tgagtttagg cttgttggtc tttaaccttg
    19981 ggcatagacg ctttgcgcat gggttttatc agtttcttgc agtggcttta ggtttttcac
    20041 ttgtctttta tccaactgcc tactatagtg ctgcaacagg aagttttggg gatgcgatta
    20101 gtggtattcg ttatcctatt gacagtattc gctttgattt tacttctaaa attttagaga
    20161 atatgttttt ttatggcttg ttgtcccttg gtttgggatt tgtggtttgt atctttttag
    20221 gtctctttca aaccaaaccc tttaaactat acgtcatttc ggttcctgca agtcttgtgg
    20281 taattttagg tttgatttta cttttctttt cacaagagcc tctgcacgct tcttatttga
    20341 tggtcgtctt ccctgttttc ctacttttat tggtaaccaa tattaagagt caacagaggg
    20401 ggcgtagtgc tagaagaagc cgaagagaaa cgccagttag cctatggagt cgtttcttca
    20461 aaggaaatct atatctgcta gtttttgggt ttgtctatct tttgtctgtt ccttttttga
    20521 tgaagtttgt cctttatcca gtaccttatc aagaacgtaa tcgtcttgct gatttggtaa
    20581 aagaggagac aaatacggaa gatactatct atgcatggga tgatactgcg actctttatc
    20641 gtaagagtga gcgcttgtcg ccatcggcga ttttgtcccc gttgcactat acagcaactg
    20701 aggaaaatcg taataagtta cttaatgact tgaaagaaaa acaacctaag gtgattgtgg
    20761 taaatgacaa ggtggtagtc tggtctgaag tggaaacact cttaaaagaa aattaccaac
    20821 aagtaaagac tgattactca gagtttaaag tctataaaat taaataacca aatcaatatc
    20881 ttgtgtattt ttaaaaattt taggattttt aacacaagat attgattttt ctttttagag
    20941 tggtataata ctttttagaa agaacatttt agaaaagagc atgcatatga ttgcactaga
    21001 agaaaaaatt acaattttgc caactctctt cgtcgagaaa cgagatggga gacgtgttgt
    21061 atttgatgtg gacaagattg acaaggctct ccacaaggcg gctgacaagg ttatggatgt
    21121 gacacccctg gttgaaaaac gcctcgatga tctgactgag cgaattatta cagaaattca
    21181 tagtcgcttt ccacagggaa ttaagattta cgaaattcaa aatatcgtag aacatgaact
    21241 ccttgaagcc aaagaatatg cgctggctga ggagtatatt acttatcgga cacagaggga
    21301 ttttgagcgc tcaaaagcga cggatatcaa ctttagtatt cataaacttc tcaacaaaga
    21361 ccagacagtt gtcaatgaaa atgctaataa agacagtgat gtctttaaca ctcagcgtga
    21421 tttgacagca gggattgttg ggaaatcaat cggactgcaa atgcttccta agcacgtagc
    21481 caatgcccac caaaaggggg atatccacta tcacgatttg gactacagtc cctatacccc
    21541 tatgaccaac tgctgtttga ttgattttaa gggtatgttg gaaaatggtt ttaagattgg
    21601 aaatgcagag gtagagagtc ccaagtctat ccaaactgcg acagcacaga tttctcaaat
    21661 cattgccaac gttgcttcta gccagtacgg tggctgttca gctgaccgta tcgatgaaat
    21721 tttggcgcct tatgcagaga agaactatca aaaacatctc aaagatgcag aagagtgggt
    21781 attgcctgaa aaacgggaag attacgcttg gaagaaaacg caaaaggaca tctacgatgc
    21841 catgcaatct cttgagtatg aaatcaatac tctcttcact tcaaatggac aaacaccttt
    21901 tacttcgcta ggttttggtc tgggaaccag tcgttttgaa cgagaaattc aaaaagctat
    21961 tttaaacatt cgcatcaagg gtcttggttc agaacaccgt acggctatct ttcctaaact
    22021 tattttcact ctgaaaagag gccttaattt agaggaagga actcccaact acgacatcaa
    22081 gcagttggct cttgagtgtg caaccaaacg gatgtatcca gatgttttat cttacgataa
    22141 gattgttgac ttgacaggat ccttcaaggt gcctatgggt tgccgttctt tcctccaagg
    22201 atggaaggat gaaaatggtg tagaagtcaa ttcaggtcgc atgaatctgg gtgttgtgac
    22261 ggttaatctg cctcgtattg ctcttgagtc tgaaggtgat atgaataagt tctgggaaat
    22321 cttcaacgag cgaatgaata tcgcagaaga tgctcttgtt taccgtgtcg aacgcactaa
    22381 agaggcgaca ccagcgaatg ctcctattct ttatcagtac ggtgcttttg gccatcgtct
    22441 aggtaaagaa gaaagtgttg accagctctt taagaatcgt cgggcaaccg tttcgctggg
    22501 ctatatcggc ttgtatgaag tagcgacagt tttctttggt aacagctggg aaagtaatcc
    22561 agatgctaag gaattcacgc tagacatcat tcacgatatg aaacgccgtg tagaagagtg
    22621 gtcagaccaa tatggctacc atttctctat ctactcaaca ccatccgaaa gtctgacaga
    22681 ccgtttctgc cgactagata tagacaagtt tggctctatt cctgatatca cagacaagga
    22741 atactacacc aactctttcc actacgatgt tcgtaaaaat ccaacaccgt ttgaaaaatt
    22801 ggactttgag aaagtctatc cagaagcagg tgcgtcaggt ggtttcatcc attattgtga
    22861 gtatccagtc cttcagcaaa atccaaaggc cttggaagct gtctgggatt atgcttatga
    22921 ccgtgtaggc tatctaggca ccaatactcc gattgaccgt tgctacaagt gtgactttga
    22981 aggggatttt gaaccaactg agagagggtt tgcttgtcca aactgtggca atagcgaccc
    23041 taaaacagta gatgtggtga agcgaacttg tggctaccta ggtaatcctc aagcaagacc
    23101 gatggtcaac gggcgtcaca aggaaatcgc tgcgcgtgtc aaacatatga atggttcaac
    23161 gattaaaata gctgggcatc aagtaacaaa ttagaaagaa atgaaatggg aaaatatcaa
    23221 ctagacgata aggggcgcgc acaagtgacc cgttatcacg agaaacactc taaaggtgga
    23281 gttggtaaga aagaacgctt gcttagcttc agagaacaat ttttaaacaa gaacaagaaa
    23341 aaataaaagt gagagccagc tctcgctttt ctcatagtgg gaggtaagga tggaattacg
    23401 cagaccaaga ttagcagata aagaaagctg ttttagatat gatgacagag tttgaaaaga
    23461 gccaatcagc ccatgatgga ggattttggg atacagagaa ctttgtgtat gaagagtggt
    23521 tggaaacaaa tattcaaaaa gagatgggaa taaacttgcc tgaaaatcgc gttccatcga
    23581 tacagttcgt attgtttggt gaatcaggtt gtgctctaag atttttgaat ctgcgattga
    23641 gactgaatga gaggttactg aattatgctg gccacattgg ctactccatt cgtccatctg
    23701 aaagaggcaa gggttatgca aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa
    23761 agaacatcaa gaaagctctg gtgacctgta gcgtgaataa tcctgctagc agagcagtca
    23821 ttctagcaaa tggtggaata tttgaggatg ctcgcaatgg agtcgagcgt tattggatag
    23881 aggtagcgaa tgaataatcc aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt
    23941 cgtatcattg actacaaggc ctttaacttt gtggacggcg aaggcgtgcg caactctctc
    24001 tatgtatcag gctgtatgtt tcactgcgag ggatgttata atgttgcgac ttggtctttt
    24061 aatgctggca ttccctatac agcagaatta gaagagcaga ttatggcaga ccttgcccaa
    24121 ccctatgttc aaggcttgac tttgctggga ggggagcctt ttctcaatac tggcattctc
    24181 ttgcctctag ttaaacgcat ccgaaaggaa ttgccagaca aggacatttg gtcctggacc
    24241 ggctacactt gggaagaaat gatgttggaa actccagata aactggaatt cttgtcactg
    24301 attgacattc ttgtcgatgg aagatatgat cgaactaaga gaaatcttat gctccagttt
    24361 cgaggttcat ctaaccaacg aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta
    24421 gtgatttggg acaagctcaa tgacggaaaa gaaagctatg aacaggtgaa gagagaatga
    24481 agaaaaaatg aagaaaaaag acttagtaga ccaactagtc tcagagatcg agacggggaa
    24541 agtcaggaca ctgggaatat acggtcatgg agcttcaggt aaatcaacct ttgcacagga
    24601 attgtaccaa gctttagatt ctactacagt aaatttgcta gaaacagatc cctatatcac
    24661 ctcaggacgc catctggtag tacccaagga cgcgccgaat caaaaggtga cagccagtct
    24721 gccagtggcg catgaactgg agagtttgca gagagatatc cttgccttgc aggcgggtat
    24781 ggatgtcttg acaattgaag aaccttggaa ggctagtgag gtcttgtctg gagccaaacc
    24841 aattttgatt gtcgaaggga tgtctgttgg ctttctaccc aaggaactct ttgaaaaaac
    24901 catctgtttc tacacggatg aggagaccga attaaagcga cgccttgcta gagatacgac
    24961 tgtgagaaat cgcgatgcat cctttatatt ggctagccat cagatgagac gggagcagta
    25021 tctgcgatac tataaagaaa ctgagtctaa ggcggatatc ttagtggacc aatcagaaga
    25081 taaatttgat gtcaagagga cttaacttat gtatagaaga aatatagaag aaaatcccta
    25141 attttagaaa gaaaaagtag gaaaacagtt tcatcccaaa aaaacgaaaa aacctaacaa
    25201 atcccttgca atcgcagggg atttgtgtta ttctattatg gtgctgtaaa ttacagcctt
    25261 agttttgatg caagaggttg cgacacgctc ggttgcattg ccacgcaaca ccgtgtcggt
    25321 tttcttgtgg agctagccta ttatcttaaa tagacgaaaa ggagaaaaag atggcaaaca
    25381 aaaaaatccg tatccgtttg aaagcttacg aacaccgtac gcttgacaca gcggctgcaa
    25441 aaatcgtaga atcagctact cgtacaggtg cacaagttgc gggtccaatc ccacttccaa
    25501 ctgaacgtag cctctacaca atcattcgtg cgactcacaa atacaaagac tctcgcgaac
    25561 aatttgaaat gcgtacacac aaacgtttga tcgatatcgt taacccaact caaaaaacag
    25621 ttgatgcctt gatgaaattg gatcttccaa gtggtgtaaa cgtagaaatc aaactttaat
    25681 ctaaaatata aaagagcaga ggctggtgtt tcaatctaat tgaacacggg ctaaactcgg
    25741 tgtgaaaaag ataaacttcc tagtgtctgc tagacactgc gtcagtttcc tattttcacg
    25801 ttgagtttga cgtcctttgt atcttagact tgagcataaa aaacgctcgt taaaaacttt
    25861 ttgaataaaa aatatagaaa aggaactatt ttctcatgac aaaaggaatc ttagggaaaa
    25921 aagtgggaat gactcaaatc ttcactgaag ctggcgaatt gatccctgta acagttattg
    25981 aagcaactcc aaacgttgtt cttcaagtta aaactgttga aacagacgga tacaacgcta
    26041 tccaagttgg tttcgatgac aaacgcgaag tattgagcaa caaacctgct aaaggacatg
    26101 tagcgaaagc taacacggct cctaagcgct tcattcgtga attcaaaaac gttgaaggct
    26161 tggaagttgg cgctgaaatc acagttgaaa cattcgcagc tggagatgtt gttgacgtaa
    26221 cgggtacttc taaaggtaaa ggtttccaag gtgttatcaa acgccacgga caatcacgtg
    26281 gaccaatggc tcacggttct cgttaccacc gtcgtccagg ttctatgggg cctgttgcac
    26341 ctaaccgcgt attcaaaggt aaaaaccttg caggacgtat gggtggcgac cgcgtaacaa
    26401 ttcaaaacct tgaagttgta caagttgttc cagaaaagaa cgttatcctt atcaaaggta
    26461 acgtaccagg tgctaagaaa tctcttatca ctatcaaatc agcagttaaa gctggtaaat
    26521 aataaagaaa ggggaaatca gtcacaatgg caaacgtaac attatttgac caaactggta
    26581 aagaagctgg ccaagttgtt cttaacgatg cagtatttgg tatcgaacca aatgaatcag
    26641 ttgtgtttga tgtaatcatc agccaacgcg caagccttcg tcaaggaaca cacgctgtta
    26701 aaaaccgctc tgcagtatca ggtggtggac gcaaaccatg gcgtcaaaaa ggaactggac
    26761 gtgctcgtca aggttctatc cgctcaccac aatggcgtgg tggtggtgtt gtcttcggac
    26821 caactccacg ttcatacggc tacaaacttc cacaaaaagt tcgtcgccta gctcttaaat
    26881 cagtttactc tgaaaaagtt gctgaaaaca aattcgtagc tgtagacgct ctttcattta
    26941 cagctccaaa aactgctgaa tttgcaaaag ttcttgcagc attgagcatc gattctaaag
    27001 ttattgttat ccttgaagaa ggaaatgaat tcgcagctct ttcagctcgt aaccttccaa
    27061 acgtgaaagt tgcaactgct acaactgcaa gtgttcttga catcgcaaat agcgacaaac
    27121 ttcttgtcac acaagcagct atctctaaaa tcgaggaggt tcttgcataa tgaatttgta
    27181 tgatgttatc aaaaaacctg tcatcactga aagctcaatg gctcaacttg aagcagggaa
    27241 atatgtattt gaagttgaca ctcgtgcaca caaacttttg atcaagcaag ctgttgaagc
    27301 tgctttcgaa ggtgttaaag ttgccaatgt taacacaatc aacgtaaaac caaaagctaa
    27361 acgtgttgga cgttacactg gttttactaa caaaactaaa aaagctatca tcacacttac
    27421 agctgattct aaagcaatcg agttgtttgc tgctgaagct gaataatcta aggaggaaat
    27481 atcgtgggaa ttcgtgttta taaaccaaca acaaacggtc gccgtaatat gacttctttg
    27541 gatttcgctg aaatcacaac aagcactcct gaaaaatcat tgcttgttgc attgaagagc
    27601 aaggctggtc gtaacaacaa cggtcgtatc acagttcgtc accaaggtgg tggacacaaa
    27661 cgtttctacc gtttggttga cttcaaacgt aataaagaca acgttgaagc agttgttaaa
    27721 acaatcgagt acgatccaaa ccgttctgca aacatcgctc ttgtacacta cactgacggt
    27781 gtgaaagcat acatcatcgc tccaaaaggt cttgaagtag gtcaacgtat cgtttcaggt
    27841 ccagaagcag atatcaaagt cggaaacgct cttccacttg ctaacatccc agttggtact
    27901 ttgattcaca acatcgagtt gaaaccaggt cgtggtggtg aattggtacg tgctgctggt
    27961 gcatctgctc aagtattggg ttctgaaggt aaatatgttc ttgttcgtct tcaatcaggt
    28021 gaagttcgta tgattcttgg aacttgccgt gctacagttg gtgttgtcgg aaacgaacaa
    28081 catggacttg taaaccttgg taaagcagga cgtagccgtt ggaaaggtat ccgcccaaca
    28141 gttcgtggtt ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtaaggca
    28201 ccagttggtc gtaaagcacc atctactcca tggggcaaac ctgctcttgg tcttaaaact
    28261 cgtaacaaga aagcgaaatc tgacaaactt atcgttcgtc gtcgcaacga gaaataatat
    28321 taaactagtc gcttaagcaa ctagtaaatc cgccagctcg gtagcgctcc ataggagtgc
    28381 aagccgctgt ggtacaacat ttaaaggaga aaatataaaa atgggacgca gtcttaaaaa
    28441 aggacctttc gtcgatgagc atttgatgaa aaaagttgaa gctcaagcta acgacgaaaa
    28501 gaaaaaagtt attaaaactt ggtcacgtcg ttcaacgatc ttcccaagtt tcattggtta
    28561 cactattgca gtttatgacg gacgtaaaca cgtacctgtt tacatccaag aagacatggt
    28621 aggccacaaa cttggtgaat ttgcaccaac tcgtacttac aaaggtcacg ctgcagacga
    28681 caagaaaaca cgtagaaaat aaggagaaca taaatggcag aaattacttc agctaaagca
    28741 atggctcgta cagtacgtgt ttcacctcgt aaatcacgtc ttgttcttga taacatccgt
    28801 ggtaaaagcg tagccgatgc aatcgcaatc ttgacattca ctccaaacaa agctgctgaa
    28861 atcatcttga aagttttgaa ctcagctgta gctaacgctg aaaacaactt tggtttggat
    28921 aaagctaact tggtagtatc tgaagcattc gcaaacgaag gaccaactat gaaacgtttc
    28981 cgtccacgtg cgaaaggttc agcttcacca atcaacaaac gtacagctca catcactgta
    29041 gctgttgcag aaaaataagg aggtaaaatc gtgggtcaaa aagtacatcc aattggtatg
    29101 cgtgtcggca tcatccgtga ttgggatgcc aaatggtatg ctgaaaaaga atacgcggat
    29161 taccttcatg aagatcttgc aatccgtaaa ttcgttcaaa aagaacttgc tgacgcagca
    29221 gtttcaacta ttgaaatcga acgcgcagta aacaaagtta acgtttcact tcacactgct
    29281 aaaccaggta tggttatcgg taaaggtggt gctaacgttg atgtactccg tgcaaaactt
    29341 aacaaattga ctggaaaaca agtacacatc aacatcatcg aaatcaaaca acctgatttg
    29401 gatgctcacc ttgtaggtga aggaattgct cgtcaattgg agcaacgtgt tgctttccgt
    29461 cgtgcacaaa aacaagcaat ccaacgtgca atgcgtgctg gagctaaagg aatcaaaact
    29521 caagtatcag gtcgtttgaa cggtgcagat atcgcccgtg ctgaaggata ctctgaagga
    29581 actgttccgc ttcacacact tcgtgcagat atcgattacg cttgggaaga agcagatact
    29641 acatacggta aacttggtgt taaagtatgg atctaccgtg gtgaagttct tccagctcgt
    29701 aaaaacacta aaggaggtaa ataaccaatg ttagtaccta aacgtgttaa acaccgtcgt
    29761 gagttccgtg gaaaaatgcg cggtgaagca aaaggtggaa aagaagtagc attcggtgaa
    29821 tacggtcttc aagctacaac tagccactgg atcactaacc gccaaatcga agctgctcgt
    29881 atcgccatga ctcgttacat gaaacgtggt ggtaaagttt ggattaaaat cttcccacac
    29941 aaatcataca ctgctaaagc tatcggtgtg cgtatgggat ctggtaaagg ggcacctgaa
    30001 ggttgggtag caccagttaa acgtggtaaa gtgatgttcg aaatcgctgg tgtatctgaa
    30061 gagattgcac gtgaagcgct tcgacttgct agccacaaat tgccagttaa atgtaaattc
    30121 gtaaaacgtg aagcagaata aggagaaggc atgaaactta atgaagtaaa agaatttgtt
    30181 aaagaacttc gtggtctttc tcaagaagaa ctcgcgaagc gcgaaaacga attgaaaaaa
    30241 gaattgtttg aacttcgttt ccaagctgct actggtcaat tggaacaaac agctcgcttg
    30301 aaagaagtta aaaaacaaat cgctcgtatc aaaacagttc aatctgaagc gaaataatag
    30361 actagggaag gagaaatttc aatggaacgc aataatcgta aagttcttgt tggacgtgtt
    30421 gtatctgaca aaatggacaa gacaatcaca gttgtagttg aaacaaaacg taaccaccca
    30481 gtctatggta aacgtattaa ctactctaaa aaatacaaag ctcatgatga aaacaatgtt
    30541 gccaaagaag gcgatatcgt acgtatcatg gaaactcgcc cgctttcagc tacaaaacgt
    30601 ttccgtcttg tagaagttgt tgaagaagcg gtcatcatct aatcaaacct gaaaggagaa
    30661 aactgaaatg attcaaacag aaactcgttt gaaagtcgca gacaacagcg gtgctcgcga
    30721 aatcttgact atcaaagttc ttggtggttc aggacgtaaa tttgcaaaca tcggtgatgt
    30781 tatcgtggca tctgtaaaac aagctactcc tggtggtgcg gttaaaaaag gtgacgttgt
    30841 taaagcagtt atcgttcgta ctaaatcagg tgctcgtcgt gctgatggtt catacatcaa
    30901 atttgacgaa aacgcagcag ttatcatccg tgaagacaaa actcctcgcg gaacacgtat
    30961 ctttggccca gttgcacgtg aattgcgtga aggtggcttc atgaagatcg tgtcacttgc
    31021 tccagaagta ctttaatttt taggaacaaa ctagtcccct agcttcaagc tagggtgccc
    31081 ttatgggcgt aagaaaaatc aaggagaaac ctaatgtttg taaaaaaagg cgacaaagtt
    31141 cgcgtaatcg ctggtaaaga caagggaaca gaagctgttg tccttactgc ccttccaaaa
    31201 gtaaacaaag ttatcgttga aggtgttaac attgttaaga aacaccaacg tccaactaac
    31261 gagcttcctc aaggtggtat catcgagaaa gaagcagcta tccacgtatc aaacgttcaa
    31321 gttttggaca aaaatggtgt agctggtcgt gttggataca aatttgtaga cggtaaaaaa
    31381 gttcgctaca acaaaaaatc aggcgaagtg cttgattaat cacgaaggaa aggagaagta
    31441 taatggcaaa tcgtttaaaa gaaaaatatc ttaatgaagt agttcctgct ttgacagaac
    31501 aattcaacta ctcatcagtg atggctgtgc ctaaagtaga taagattgtt ttgaacatgg
    31561 gtgttggtga agctgtatca aacgctaaaa gccttgaaaa agctgctgaa gaattggcac
    31621 ttatctcagg tcaaaaacca cttatcacta aagctaaaaa atcaatcgcc ggcttccgtc
    31681 ttcgtgaagg tgttgcgatc ggtgcaaaag ttacccttcg tggtgaacgt atgtacgaat
    31741 tcttggataa attggtatca gtttcacttc cacgtgtacg tgacttccac ggtgtcccaa
    31801 caaaatcatt tgatggacgc gggaactaca cacttggtgt gaaagaacaa ttaatcttcc
    31861 cagaaatcaa cttcgatgac gttgacaaaa ctcgtggtct tgacatcgtt atcgtaacaa
    31921 ctgctaacac tgacgaagag tcacgtgcat tgcttacagg ccttggaatg ccttttgcaa
    31981 aataatatag gaggtaaatc taatggctaa aaaatcaatg gtagctagag aggctaaacg
    32041 ccaaaaaatt gttgaccgtt atgctgaaaa acgtgctgca ttaaaggcgg caggggacta
    32101 cgaaggttta tctaaattac ctcgcaacgc ctcaccgact cgtttacata atcgttgtag
    32161 agttacgggg cgcccacatt cagtttaccg caaatttggt ctgagtcgta tcgcttttcg
    32221 cgaacttgcg cataaaggtc aaattcctgg tgtaacaaaa gcatcttggt aatttaagat
    32281 atcaagggtg tcaaaactcc aagtaaaaat aggaaacttg acgaagaaac taaagtttct
    32341 aggaaagttt atctttttca cacagagttt agcccgggtt caattgggct tgccaatttg
    32401 aacccgagct acagctttgg caaaaaagac caatttgctt tggagcattg cttctgcatt
    32461 aaattgtcta tttttgctcg tgctgttacg ctctttgtat catgtattaa ctagcaagtg
    32521 caacttgcaa actactagta agaggagaaa aacaaaatgg ttatgactga cccaatcgca
    32581 gacttcctaa ctcgtattcg taatgctaac caagctaaac acgaagtact tgaagtacct
    32641 gcatcaaaca tcaaaaaagg gattgctgaa atccttaaac gcgaaggttt tgtaaaaaac
    32701 gttgaaatca ttgaagatga caaacaaggc gtcatccgtg tatttcttaa atacggatca
    32761 aatggtgaga aagttatcac taacttgaaa cgtgtttcta aaccaggact tcgtgtctac
    32821 aaaaaacgtg aagaccttcc aaaagttctt aacggacttg gaattgccat cctttcaact
    32881 tctgaaggtt tgcttactga taaagaagca cgccaaaaga atgttggtgg tgaggttatc
    32941 gcttacgttt ggtaaaatca agatacaaag ctcgtaaaga acaaagcaaa attaggaagt
    33001 tggagaagtt tgtttacaaa caagccaact tatctatttt gcacagttct tagagcgtgt
    33061 tcagttcagc tcttgaacta aataagtatc tgaaccccgt gaaaactggc cgttctggcc
    33121 tgacaattta acaggagaaa ataaacatgt cacgtattgg taataaagtt atcgtgttgc
    33181 ctgctggtgt tgaactcgct aacaatgaca acgttgtaac tgtaaaagga cctaaaggag
    33241 aacttactcg tgagttctca aaagatattg aaatccgtgt ggaaggtact gaaataactc
    33301 ttcaccgtcc aaacgattca aaagaaatga aaactatcca cggaactact cgtgcccttt
    33361 tgaacaacat ggttgttggt gtatcagaag gattcaagaa agaacttgaa atgcgtgggg
    33421 ttggttaccg tgcacagctt caaggatcta aacttgtttt ggctgttggt aaatctcatc
    33481 cagacgaagt tgaagctcca gaaggaatta cttttgaact tccaaaccca acaacaatcg
    33541 ttgttagcgg aatttcaaaa gaagtagttg gtcaaacagc tgcttacgta cgtagccttc
    33601 gttcaccaga accatataaa ggtaaaggta tccgttacgt tggtgaattc gttcgccgta
    33661 aagaaggtaa aacaggtaaa taatgttgag tggttgatca tcaaccacca acctattttc
    33721 caactttgtg catagcacac gatttaaaac taaagaggtg aaaactgtga tttcaaaacc
    33781 agataaaaac aaactccgcc aaaaacgcca ccgtcgcgtt cgcggaaaac tctctggaac
    33841 tgctgatcgc ccacgtttga acgtattccg ttctaataca ggcatctacg ctcaagtgat
    33901 tgatgacgta gcgggtgtaa cgctcgcaag tgcttcaact cttgataaag aagtttcaaa
    33961 aggaactaaa actgaacaag ccgttgctgt cggtaaactc gttgcagaac gtgcaaacgc
    34021 taaaggtatt tcagaagtgg tgttcgaccg cggtggatat ctatatcacg gacgtgtgaa
    34081 agctttggct gatgcagctc gtgaaaacgg attgaaattc taataggagg acactagaaa
    34141 atggcattta aagacaatgc agttgaatta gaagaacgcg tagttgctgt caaccgtgtt
    34201 acaaaagttg ttaaaggtgg acgtcgtctt cgtttcgcag ctcttgttgt tgttggtgac
    34261 cacaatggtc gcgtaggatt tggtactggt aaagctcaag aagttccaga agcaatccgt
    34321 aaagcagtag atgatgctaa gaaaaacttg atcgaagttc ctatggttgg aacaacaatc
    34381 ccacacgaag ttctttcaga attcggtgga gctaaagtat tgttgaaacc tgctgtagaa
    34441 ggttctggag ttgccgctgg tggtgcagtt cgtgccgttg tggaattggc aggtgtggca
    34501 gatattacat ctaaatcact tggttctaac actccaatca acattgttcg tgcaactgtt
    34561 gaaggtttga aacaattgaa acgcgctgaa gaaattgctg cccttcgtgg tatttcagtt
    34621 tctgatttgg cataagaaag gggataaaat ggctcaaatt aaaattactt tgactaagtc
    34681 tccaatcgga cgcattccat cacaacgtaa aactgttgta gcacttggac ttggcaaatt
    34741 gaacagctct gttattaaag aagataacgc tgctatccgt ggtatgatca cagcagtatc
    34801 tcacttagta acagttgaag aagtaaacta atgaagtttt aggggatgtg cactgtacca
    34861 tcccctaaaa ctagatatag tcatctatga tgacatcgta taggcgagtt gatgggggag
    34921 acaacctttt ctcccttatc ggcgctagca ttttacaaaa gaggagaaaa taaaaatgaa
    34981 acttcatgaa ttgaaacctg cagaaggttc tcgtaaagta cgtaaccgcg ttggtcgtgg
    35041 tacttcatca ggtaacggta aaacatctgg tcgtggtcaa aaaggtcaaa aagctcgtag
    35101 cggtggcgga gttcgccttg gttttgaagg tggacaaact ccattgttcc gtcgtcttcc
    35161 aaaacgtgga ttcactaaca tcaacgctaa agaatacgca attgtgaacc ttgaccaatt
    35221 gaacgtcttt gaagatggtg ctgaagtaac tccagttgtt cttatcgaag caggaattgt
    35281 taaagctgaa aagtcaggta ttaaaattct tggtaacggt gagttgacta agaaattgac
    35341 tgtgaaagca gctaaattct ctaaatcagc tgaagaagct atcactgcta aaggtggttc
    35401 agtagaagtc atctaagaga ggtgacctat gttttttaaa ttattaagag aagctcttaa
    35461 agtcaagcag gttcgatcaa aaattttatt tacaattttt atcgttttgg tctttcgtat
    35521 cggaactagc attacagttc ctggtgtgaa tgccaatagc ttgaatgctt taagtggatt
    35581 atccttctta aacatgttga gcttggtgtc ggggaatgcc ctaaaaaact tttcgatttt
    35641 tgccctagga gttagtccct atatcactgc ttctattgtt gtccaactct tgcaaatgga
    35701 tattttaccc aagtttgtag agtggggtaa acaaggggaa gtaggtcgaa gaaaattgaa
    35761 tcaagctact cgttatattg ctctagttct cgcttttgtg caatctatcg ggattacagc
    35821 tggttttaat accttggctg gagctcaatt gattaaaact gctttaactc cacaagtttt
    35881 tctgacgatt ggtatcatct taacagctgg tagtatgatt gtcacttggt tgggtgagca
    35941 aattacagat aagggatacg gaaacggtgt ttccatgatt atctttgccg ggattgtttc
    36001 ttcaattcca gagatgattc agggcatcta tgtggactac tttgtgaacg tcccaagtag
    36061 ccgtatcact tcatctatca ttttcgtaat cattttgatt attactgtat tgttgattat
    36121 ttactttaca acttatgttc aacaagcaga atacaaaatt ccaatccaat atactaaggt
    36181 tgcacaaggt gctccatcta gctcttacct tccgttaaag gtaaaccctg ctggagttat
    36241 ccctgttatc tttgccagtt cgattactgc agcgcctgcg gctattcttc agtttttgag
    36301 tgccacaggt catgattggg cttgggtaag ggtagcacaa gagatgttgg caactacttc
    36361 tccaactggt attgccatgt atgctttgtt gattattctc tttacattct tctatacgtt
    36421 tgtacagatt aatcctgaaa aagcagcaga gagcctacaa aagagtggtg cctatatcca
    36481 tggagttcgt cctggtaaag gtacagaaga atatatgtct aaacttcttc gtcgtcttgc
    36541 aactgttggt tccctcttcc ttggtgtgat ttccatttta ccgattgcag ctaaagatgt
    36601 atttggtctt tctgatgttg ttgcctttgg tggaacaagt ctcttgatca ttatctctac
    36661 aggtatcgaa ggaatcaagc aattggaagg ttacctattg aaacgtaagt atgttggttt
    36721 catggacaga acagaataaa agtatttact gaatcagtaa atgctgaggg agtggaggtt
    36781 taaactctga catttgtaag agttggatct cccctcttct attttgtttt taaatagggg
    36841 tgaaaagact ttttgcttct atttaaaaat aaaataagga gatcaaatca tgaatctttt
    36901 gattatgggc ttacctggtg caggtaaggg aactcaagca gcaaaaatcg tagaacaatt
    36961 ccatgttgca catatctcaa caggtgatat gttccgcgct gcaatggcaa atcaaactga
    37021 aatgggtgtt cttgctaagt catatattga caagggtgaa ttggttcctg acgaagttac
    37081 aaatggaatc gtaaaagaac gcctttcaca agatgatatt aaagaaacag gattcttatt
    37141 ggatggttac ccacgtacaa ttgaacaagc tcatgccttg gacaaaacat tggctgaact
    37201 tggcattgaa ctagaaggtg ttatcaatat tgaagtgaac cctgacagcc tcttggaacg
    37261 tttgagtggc cgtatcatcc accgcgtaac tggagaaact ttccacaagg tctttaaccc
    37321 accagttgac tataaagaag aagattacta ccaacgtgaa gatgataagc ctgagacagt
    37381 aaaacgtcgt ttggatgtta atattgctca aggagaacca atcattgctc actaccgtgc
    37441 caaaggtttg gttcatgaca tcgaaggtaa tcaagatatc aatgatgtct tctcagatat
    37501 tgaaaaagta ttgacaaatt tgaaataaag cgtttttcat acttgcaaaa attcgctaca
    37561 aatgttatac tgagatagtc tgacttataa ttgttgtctc tgtgtctaga ggcatcgaat
    37621 cgaaatttat ggaggtgctt ttgcgtggca aaagacgatg tgattgaagt tgaaggcaaa
    37681 gtagttgata caatgccgaa tgcaatgttt acggttgaac ttgaaaatgg acatcagatt
    37741 ttagcaacag tttctggtaa aattcgtaaa aactatattc gtattttagc gggagatcgt
    37801 gttactgtcg aaatgagtcc atatgacttg acacgtggac gtatcactta ccgctttaaa
    37861 taatcgaaaa acttggaggg ataagaaatg aaagtaagac catcggtcaa accaatttgc
    37921 gaatactgta aagttattcg tcgtaatggt cgtgttatgg taatttgccc agcaaatcca
    37981 aaacacaaac aacgtcaagg ataagataga aaggagaaaa catggctcgt attgctggag
    38041 ttgatattcc aaatgacaaa cgcgtagtaa tctcattgac ttatgtttat ggtatcggac
    38101 ttgcaacatc taagaaaatt ttggctgctg ctggaatctc agaagatgtt cgtgtacgtg
    38161 atcttacatc agatcaagaa gatgctatcc gtcgtgaagt ggatgcaatc aaagttgaag
    38221 gtgaccttcg tcgtgaagta aacttgaaca tcaaacgttt gatggaaatc ggttcatacc
    38281 gtggtatccg tcaccgtcgt ggacttcctg tccgtggaca aaacactaaa aacaacgctc
    38341 gcactcgtaa aggtaaagct gttgcgattg ctggtaagaa aaaataatat aggaggtaaa
    38401 agtcttggct aaaccaacac gtaaacgtcg tgtgaaaaag aatatcgaat ctggtattgc
    38461 tcatattcac gctacattta ataacactat tgttatgatt actgatgtgc atggtaatgc
    38521 aattgcttgg tcatcagctg gtgctcttgg tttcaaaggt tctcgtaaat ctacaccatt
    38581 cgctgctcaa atggcttctg aagctgctgc taaatctgca caagaacacg gtcttaaatc
    38641 agttgaagtt actgtaaaag gtccaggttc tggtcgtgag tcagctattc gtgcgcttgc
    38701 tgccgctggt cttgaagtaa cagcaattcg tgatgtgact ccagtgccac acaatggtgc
    38761 tcgtcctcca aaacgtcgcc gtgtataatc atcgcattac actgcttttc gtttaagagg
    38821 gagtaactaa atgatcgagt ttgaaaaacc aaatataaca aaaattgatg aaaataaaga
    38881 ttatggcaag tttgtaatcg aaccacttga acgtggctac ggtacaactc ttggtaactc
    38941 tcttcgtcgt gtacttctag cttctctacc aggagcagct gtgacatcta tcaacattga
    39001 tggtgtgtta catgagtttg acacagttcc aggtgttcgt gaagacgtga tgcaaatcat
    39061 tctgaacatt aaaggaattg cagtgaaatc gtacgttgaa gacgaaaaaa tcatcgaact
    39121 ggatgttgaa ggtcctgctg aagtaacagc tggtgacatt ttgacagata gcgatattga
    39181 aattgtaaat ccagatcatt atctctttac aatcggtgaa ggttcttctc taaaagcgac
    39241 tatgactgtt aacagtggtc gtggatatgt acctgctgat gaaaataaaa aggataatgc
    39301 accagttgga acacttgctg tagattctat ttatacacca gttacaaaag tcaactatca
    39361 agtggaacct gctcgtgtag gtagcaatga tggtttcgac aaattaaccc ttgaaatctt
    39421 gacaaatgga acaattattc cagaagatgc tttagggctt tcagcacgta ttttgacaga
    39481 acatcttgat ttgtttacaa atcttactga gattgctaag tcaactgaag tgatgaaaga
    39541 agctgatact gaatctgacg accgtatttt agatcgtacg attgaggaac tggacttgtc
    39601 tgtgcgttca tacaactgtt taaaacgtgc cggtatcaat actgtgcatg atttgacaga
    39661 aaaatctgaa gcagagatga tgaaagtacg aaatcttgga cgcaagagtt tggaagaagt
    39721 gaaactcaaa ctcattgatt tgggtcttgg attaaaagat aaataaagga ggaatacatg
    39781 gcttaccgta aactaggacg cactagctca caacgtaaag caatgcttcg cgatttgaca
    39841 actgaccttt tgatcaacga atcaatcgtg acaactgaag ctcgtgctaa agaaatccgt
    39901 aaaactgttg aaaaaatgat tactctaggt aaacgtggtg atttgcatgc acgtcgtcaa
    39961 gcagctgctt tcgtacgtaa tgaaatcgca tctgaaaact atgatgaagc aactgataag
    40021 tacacttcta ctacagcact tcaaaaattg ttctcagaaa tcgcacctcg ttatgctgaa
    40081 cgtaacggtg gatacactcg tatccttaaa actgaaccac gtcgtggtga tgcagcgcca
    40141 atggcgatca tcgaattagt ataaaatcat caattttgtt gagtgttatg atgatggagt
    40201 cttgtgctct tagtctagct ctggtctacc gctaggattt cggtcctagc gggaacactc
    40261 atcataagtt gggatagtag acgcttgttt acgaaattgt ttttttctta agaacaactt
    40321 cgtaagcagg cgtttttgag tattttcgtt agaattatgc tatactattt gaaaagaatc
    40381 ctgtttaatg ttaaggtttc ttattttaag aagaattgga gtttacttat gaaagccatt
    40441 ataactgttg ttggtaaaga taaatctgga attgttgcag gtgtttctgg taaaattgca
    40501 gaattaggat tgaatattga cgatatctct caaactgtct tagatgaata ttttacgatg
    40561 atggctgttg tatctagtga tgaaaagcaa gattttacct attttcgtaa tgaatttgaa
    40621 gcttttgggc aaactttgaa tgtaaaaatc aatattcaga gtgcagcgat tttcgaagct
    40681 atgtataata tctaggaggt tatcatggat attagacaag ttactgaaac catcgccatg
    40741 attgaggagc aaaatttcga tatcagaacc attaccatgg ggatttctct tttggactgt
    40801 atcgatccag atatcaatcg tgctgcggag aaaatctatc aaaaaattac gacaaaggcg
    40861 gctaatttag tagctgttgg tgatgaaatt gcggctgagt tgggaattcc tatcgttaat
    40921 aagcgtgtat cggtgacacc tatttctctg attggggcag cgacagatgc gacggactac
    40981 gtggttctgg caaaagcgct tgataaggct gcgaaagaga ttggtgtgga ctttattggt
    41041 ggtttttctg ccttagtaca aaaaggttat caaaagggag atgagattct catcaattcc
    41101 attcctcgcg ctttggctga gacggataag gtctgctcgt cagtcaatat cggctcaacc
    41161 aagtctggta ttaatatgac ggctgtggca gatatgggac gaattatcaa ggaaacagca
    41221 aatctttcag atatgggagt ggccaagttg gttgtattcg ctaatgctgt tgaggacaat
    41281 ccatttatgg cgggtgcctt tcatggtgtt ggggaagcag atgttatcat caatgtcgga
    41341 gtttctggtc ctggtgttgt gaaacgtgct ttggaaaaag ttcgtggaca gagctttgat
    41401 gtagtagccg aaacagttaa gaaaactgcc tttaaaatca ctcgtatcgg tcaattggtt
    41461 ggtcaaatgg ccagtgagag actgggtgtg gagtttggta ttgtggactt gagtttggca
    41521 ccaacccctg cggttggaga ctctgtggca cgtgtccttg aggaaatggg gctagaaaca
    41581 gttggcacgc atggaacgac ggctgccttg gccctcttga acgaccaagt taaaaagggt
    41641 ggagtgatgg cctgcaacca agtcggtggt ttatctggcg cctttatccc tgtttctgag
    41701 gatgaaggaa tgattgctgc agtgcaaaat ggctctctta atttagaaaa actagaagct
    41761 atgacggcta tctgttctgt tggattggat atgattgcca tcccagaaga tacgcctgct
    41821 gaaaccattg cggctatgat tgcggatgaa gcagcaatcg gtgttatcaa catgaaaaca
    41881 acagctgttc gtatcattcc caaaggaaaa gaaggcgata tgattgagtt tggtggtcta
    41941 ttaggaactg cacccgttat gagggttaat ggggcttcgt ctgtcgactt catctctcgc
    42001 ggtggacaaa tcccagcacc aattcatagt tttaaaaatt aagaaaatag gagaaatttt
    42061 aagttctatt taagattaga tgtgtatact ataatcatta aataaagacc tcctaatatt
    42121 atttgaaaca gataacactg aattagtttg aatttgattt tcatctaata tctttattta
    42181 atgaactcct aaactttttc ataataatct ccttcaaaag tcgcctgtat gggtggcttt
    42241 tattttatca ttcatgatat aatagaagca aacggaggac ggaaaatggt aaaagtacga
    42301 ttgtatttgg tacgtcatgg caagaccatg tttaacacga ttggtcgcgc gcaaggttgg
    42361 agcgatactc ccttaactgc tgaaggtgaa cgagggattc aagagttagg aatcggtttg
    42421 cgagaatctg atctacagtt tgagcgcgct tattcgagtg attctggtcg taccattcag
    42481 accatgggaa ttatacttga agaacttggc ttgcagggga aaatccctta tcgcatggac
    42541 aagcgtatca gagaatggtg tttcggtagt tttgatggag cctatgatgg tgatcttttc
    42601 atgggcatta ttcctcgtat ctttaatgtg gaccacgttc accaattgtc ttatgctgaa
    42661 ctggctgagg gcttggtaga ggtcgataca gctggttggg ctgaaggttg ggaaaaactc
    42721 agtggccgaa tcaaggaagg ctttgaaata attgcaaaag aaatggaaga tcaaggtgga
    42781 ggtaacgccc ttgttgtcag ccatggaatg actattggaa ccattgttta tctgattaat
    42841 ggcatgcatc cgcatggtct agataatggt agcgtgacga ttcttgaata tgaggacggt
    42901 cagtttaggg ttgaagttgt cggtgaccgt agttaccgag agctaggacg tgagaagatg
    42961 gaagaaggct ctatttaatc agtctagact tgcttgccat gagctaggga tttgataaga
    43021 atatcaagat aagaaaaaac agccgagggt actcctttcg gctgtttttg atgtggaaaa
    43081 ctaaagtgta atgctattgc ttttagagat tttcataaac aagagcaagg aacctactgt
    43141 tagaacagtc aggatagttg acaaggctgc ggctacaccg taatttcctc tgagaacctc
    43201 tgtataaata gctacagtca ttgttcttgt tttgacattg tagaggagga tagaggatag
    43261 aagtagagag ttctgaaatc agtgtgaccc aagataggat ggctccagaa ataatacccg
    43321 atagcatcat tggagttgta atcttagcaa aggtattgag acgactactt cctaagcttt
    43381 cagcagcttc ttcaatactt ggtgctattt gttgtaagct agcaacagat gagcgaatag
    43441 tatacggtaa tcttctgaca gatagagaca taatcaagat gaaagcagtc cctgtaatca
    43501 taagaaatcc acttccaaat agaccagtat tgaaggaaga aatgaaggca atccctagaa
    43561 cggttcctgg tacaatataa ggtaccatac tgaggctgtc aattaagttt gtaaacaaat
    43621 tccgttttct aacggctagg taggagataa atgtcgcaaa tagaacaact agaactaagg
    43681 caatcaaagg gatacgaatg gtattgaaaa tagcagatcc catacgattg aaagctagct
    43741 tgtaactgtt tggagaataa tctttaaaaa ataccatacc tgatgttttt aggaaagagg
    43801 tataaattaa gtagatttga ggtaaaacag agataaagat aattccgtag actgttgcat
    43861 aaatggcagc catttttcct tttgtagttt ttttaggctc aattggatgg agcagattca
    43921 tgctgaaact gtagcggttt gcaatgtgtt tttggataag gaaaattgcc aaggcaatga
    43981 taatcgccat aattgcaaaa gcagaatttc ctccaacctc gctaataaat tgggtataaa
    44041 tcaggacagg gaaagtccga tatccttcgc caatcaacat aggcgttcca aagtctgaga
    44101 atgctctcat aaatacaagc aaggagctgc tagtaaggtt ggaactagaa gaggtaaaac
    44161 aaccgttacg ataggtttaa atccgaagga ccccatgctt tcagctgctt caagtagaga
    44221 attgtcaata ctgttcattg ttccagcaac atatagaaat accagtggga atagttgcag
    44281 tgtaaagaca agtacaattc ctttgaatcc ataaatatcg atagctggaa gatgaagggc
    44341 atttgtcaaa aatttagtga tgaccccatt tcgtcccagc aagagaaccc aggagtaggc
    44401 tcctacgaaa ggagctgaca tggaagcaat gataatcaat atttgtagaa atttcttccc
    44461 cttgaagtca tacatagaga agagataagc taatagggtt cctacaacta aggaagtgac
    44521 agtagcggta atggaaaccc tgaaactgtt gactagtgtc tcagagtagt aggttttact
    44581 aaagaaagtg acaaaattag ctagtgagaa ttgtccttca tgtataagtg cttacttgag
    44641 cacggtaacg ataggataaa cgagaaagac aagataggta agaaagatga agaaagagga
    44701 ggctgcccaa atatttagtt ttttacattc catggttgac tccttttatc aggttttggg
    44761 aaccatctgc agaaaagatg tttcattttt gcgtattgat tcgtagacga agacgattgc
    44821 ctttttgtag atcttcttca aaagttgatt cttcactaac ttgaattttt gaggcaaaac
    44881 ctgtctcaat gaaatactcc gtatttagtc caagatagac gctatcgcta atagttcctt
    44941 caatatctcc agattcatct ttgataaact cttcgggacg aatgcttaca tgaatagctt
    45001 gctcctcaac ctgatcaaga gctggcattc gaagggcata gccatctgaa aagacgatat
    45061 aagcgccgtc gctccgtttt tcaagattgg cagggataat atttgtgcgt ccgataaagg
    45121 ttgccacaaa ctcattagct ggtttatgat agagttcttt tggtcggccg atttgttgga
    45181 tcaccccatc tttcataaca gcaatttggt ctgaaatagc catggcttct tcttggtcgt
    45241 gggttacata aacagttgta attcccactt cgtgttggat ttctcggatg gcttgacgca
    45301 tatccaagcg aagtttggcc tccagattac taagtggctc gtccatgagg agaacacttg
    45361 gattaaccgc taaggcgcat gccaaggtga cacgttgttg ttgtccacca ctgagtttat
    45421 cgggctttca atccgcatat tgagcaattt gcatgagttc aagatacttg ttggtctgtt
    45481 gaatcaattc ttcttttgga accttctttt gcataagacc aaaagcaacg ttgtctcgga
    45541 cagtcaaatg tgggaaaata gcgtagtttt ggaaaaccag cccgatattg cgtttgctgg
    45601 gttccatatt attgattttt gtatcatcga agtaaaattc tccatctttg atactgttga
    45661 aacctgcaat catacgaaga agggtcgttt tcccacatcc tgaagctcca agaagggtaa
    45721 agagacttcc ttttggaatt gtaatgttca aattctcaat aacagggaca tcgtggtaga
    45781 tttttttggc gttaataatt tcgatctcac tcatagtgaa cctcttttac tgtttagatt
    45841 ggatatctgt aaagacttcg ttgtatttct taacgatatc tgatttattc ttgatgacat
    45901 aatcataatc ttcagtgagt gttttgattt tgtcaattgg tttcatgttt tcgcttgttt
    45961 tagcattttt acgaacagga cggttagtag tggttgtacc aagtgtatct tgtacttctt
    46021 gagagataat aaaatcgata aatttcttgg cattttccat atttttagct tttttaacga
    46081 tagcagcact agcaggtagg aagacggttc cttcttttgg atagactacc ttaatgttag
    46141 ctccgtcatt taagagttta actgctggat cctcataaga gagaccaaca gccatttctc
    46201 catcaacgac tgctttatag acaccagata aacttaaacc gattttatag tgtattgaaa
    46261 ctagaatagt acacatctac ttctaaaata ttgttagaaa tcgatttgaa tgtcctgatt
    46321 aatttgtcct gttcttattt cattttatta taccatcaat aagtgtgaaa agatctttta
    46381 cataagacca agccttatca tctttgtaac caccttgagc ttgtagcata tttgttaatt
    46441 gagcaaaggc gctagaagag tttgctgggt cagcagttgc gatttttcct tttagttcag
    46501 gtttgagaag gtcgctatat ccttcgatgt tcatgccttt agttaaatca gggtggacga
    46561 ttaaaacatt accatctagt gtataaggag tagagaagcc agttgtgttt tgatattctt
    46621 tgataacatt atcattttct tttgaaatat agttttcaaa gagttctccg tgggtagcat
    46681 attgtgtata agaaccacca aagataacat cagctacagg aacttctttt tctgactcta
    46741 gttttttgaa aagttctcca gtaccagctt gaatcagttc tattttgata ccatattttt
    46801 cttcaaaggc aggaatagtt gctccaatta agccttctga gtttggtcaa taaacgacta
    46861 gcgaaccacc gtctccttta tcagatgaac tgtcatcggc agattcatta gaagaacaag
    46921 cagcataata catccatttc tttttcatga tggatacctc cgttgtgtta tttaagttta
    46981 ttttaaaaca atgtaagcgt ttttaaaacc tacaattcta ttctatagtg tattgaatct
    47041 ataacagtac actttgactg ctaaaatatt tctataaatt aatttgactt tcctgataga
    47101 gatgttcaca tcttatttca attcactata ttagagtaaa attctctaca aaaagaagaa
    47161 tagcctattt tactattctt ccgagtgatt tcaatttctt tggggaaata tggagatact
    47221 ttttaaatac tttggcaaaa tatttatagt ctgaaaatcc aacttggtca gagatatcgc
    47281 ttaaagagga gttgggagat tcagccatct tatctatagc ttgtttcagg cgatattgta
    47341 agatatattc atttagagtc atggggaagt cttctttgat aaccttatat agatgcttgt
    47401 tatctgcaaa tagctagagc atttcaagga aataaactag aaataacaat taatatggtg
    47461 gttcagaaat accttttgat aatcaataag atcttcagga tgaagtgcgt tgactccatc
    47521 catttcatat ttccgattta ataaacgata tttgttttca ccaaattgat gaaaagggag
    47581 tagttgaact tggtcgatat ttaatgagtt aaatagagta gcgaattttt ctgcatcctc
    47641 taaactattg ttaaaattag gaataactgg gattcttaaa acg
//
LOCUS       DAVKZB010000005        41327 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKZB010000005 DAVKZB010000000
VERSION     DAVKZB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.005"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(43..378)
                     /locus_tag="VXA80_000247"
     CDS             complement(43..378)
                     /locus_tag="VXA80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352806.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(469..1779)
                     /gene="der"
                     /locus_tag="VXA80_000248"
     CDS             complement(469..1779)
                     /gene="der"
                     /locus_tag="VXA80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV0352807.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(1793..2506)
                     /locus_tag="VXA80_000249"
     CDS             complement(1793..2506)
                     /locus_tag="VXA80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV0352808.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(2503..3399)
                     /gene="dnaI"
                     /locus_tag="VXA80_000250"
     CDS             complement(2503..3399)
                     /gene="dnaI"
                     /locus_tag="VXA80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV0352809.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3400..4569)
                     /locus_tag="VXA80_000251"
     CDS             complement(3400..4569)
                     /locus_tag="VXA80_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV0352810.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(4570..5043)
                     /gene="nrdR"
                     /locus_tag="VXA80_000252"
     CDS             complement(4570..5043)
                     /gene="nrdR"
                     /locus_tag="VXA80_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV0352811.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5183..5548
                     /locus_tag="VXA80_000253"
     CDS             5183..5548
                     /locus_tag="VXA80_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0352812.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            5553..6248
                     /locus_tag="VXA80_000254"
     CDS             5553..6248
                     /locus_tag="VXA80_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352813.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6265..7032
                     /locus_tag="VXA80_000255"
     CDS             6265..7032
                     /locus_tag="VXA80_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352814.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7385..8584)
                     /locus_tag="VXA80_000256"
     CDS             complement(7385..8584)
                     /locus_tag="VXA80_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352815.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMSKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(8581..9474)
                     /locus_tag="VXA80_000257"
     CDS             complement(8581..9474)
                     /locus_tag="VXA80_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352816.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            9590..9670
                     /locus_tag="VXA80_000258"
                     /pseudo
     CDS             9590..9670
                     /locus_tag="VXA80_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10180..10368
                     /locus_tag="VXA80_000259"
                     /pseudo
     CDS             10180..10368
                     /locus_tag="VXA80_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            10783..11400
                     /locus_tag="VXA80_000260"
     CDS             10783..11400
                     /locus_tag="VXA80_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV0352817.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(11720..12607)
                     /locus_tag="VXA80_000261"
     CDS             complement(11720..12607)
                     /locus_tag="VXA80_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0352818.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(12756..14639)
                     /locus_tag="VXA80_000262"
     CDS             complement(12756..14639)
                     /locus_tag="VXA80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0352819.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVREVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            15014..16468
                     /locus_tag="VXA80_000263"
     CDS             15014..16468
                     /locus_tag="VXA80_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0352820.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            16449..17414
                     /locus_tag="VXA80_000264"
     CDS             16449..17414
                     /locus_tag="VXA80_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0352821.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(17450..18724)
                     /locus_tag="VXA80_000265"
     CDS             complement(17450..18724)
                     /locus_tag="VXA80_000265"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV0352822.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(18724..19896)
                     /locus_tag="VXA80_000266"
     CDS             complement(18724..19896)
                     /locus_tag="VXA80_000266"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV0352823.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     ASLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20027..20275)
                     /locus_tag="VXA80_000267"
     CDS             complement(20027..20275)
                     /locus_tag="VXA80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352824.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(20343..20967)
                     /locus_tag="VXA80_000268"
                     /pseudo
     CDS             complement(20343..20967)
                     /locus_tag="VXA80_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(21029..21478)
                     /locus_tag="VXA80_000269"
     CDS             complement(21029..21478)
                     /locus_tag="VXA80_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0352825.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(21480..22238)
                     /locus_tag="VXA80_000270"
     CDS             complement(21480..22238)
                     /locus_tag="VXA80_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0352826.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(22353..24332)
                     /gene="pknB"
                     /locus_tag="VXA80_000271"
     CDS             complement(22353..24332)
                     /gene="pknB"
                     /locus_tag="VXA80_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV0352827.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIILTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(24329..25069)
                     /locus_tag="VXA80_000272"
     CDS             complement(24329..25069)
                     /locus_tag="VXA80_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV0352828.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(25084..26397)
                     /gene="rsmB"
                     /locus_tag="VXA80_000273"
     CDS             complement(25084..26397)
                     /gene="rsmB"
                     /locus_tag="VXA80_000273"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV0352829.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVALKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(26390..27325)
                     /locus_tag="VXA80_000274"
     CDS             complement(26390..27325)
                     /locus_tag="VXA80_000274"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV0352830.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(27338..29734)
                     /locus_tag="VXA80_000275"
     CDS             complement(27338..29734)
                     /locus_tag="VXA80_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV0352831.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(29800..30114)
                     /locus_tag="VXA80_000276"
     CDS             complement(29800..30114)
                     /locus_tag="VXA80_000276"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV0352832.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(30139..30765)
                     /gene="gmk"
                     /locus_tag="VXA80_000277"
     CDS             complement(30139..30765)
                     /gene="gmk"
                     /locus_tag="VXA80_000277"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV0352833.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(30895..32508)
                     /locus_tag="VXA80_000278"
     CDS             complement(30895..32508)
                     /locus_tag="VXA80_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV0352834.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(32699..32953)
                     /locus_tag="VXA80_000279"
     CDS             complement(32699..32953)
                     /locus_tag="VXA80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV0352835.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(32957..33211)
                     /locus_tag="VXA80_000280"
     CDS             complement(32957..33211)
                     /locus_tag="VXA80_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV0352836.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(33449..34546)
                     /locus_tag="VXA80_000281"
     CDS             complement(33449..34546)
                     /locus_tag="VXA80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV0352837.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLEQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(34556..35302)
                     /locus_tag="VXA80_000282"
     CDS             complement(34556..35302)
                     /locus_tag="VXA80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0352838.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFVEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(35405..35758)
                     /gene="rsfS"
                     /locus_tag="VXA80_000283"
     CDS             complement(35405..35758)
                     /gene="rsfS"
                     /locus_tag="VXA80_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV0352839.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(35771..36271)
                     /locus_tag="VXA80_000284"
     CDS             complement(35771..36271)
                     /locus_tag="VXA80_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0352840.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(36272..36865)
                     /gene="yqeK"
                     /locus_tag="VXA80_000285"
     CDS             complement(36272..36865)
                     /gene="yqeK"
                     /locus_tag="VXA80_000285"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV0352841.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(36865..37494)
                     /locus_tag="VXA80_000286"
     CDS             complement(36865..37494)
                     /locus_tag="VXA80_000286"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV0352842.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(37546..37857)
                     /gene="yhbY"
                     /locus_tag="VXA80_000287"
     CDS             complement(37546..37857)
                     /gene="yhbY"
                     /locus_tag="VXA80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV0352843.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(38071..39177)
                     /gene="yqeH"
                     /locus_tag="VXA80_000288"
     CDS             complement(38071..39177)
                     /gene="yqeH"
                     /locus_tag="VXA80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV0352844.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKYAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(39180..39770)
                     /locus_tag="VXA80_000289"
     CDS             complement(39180..39770)
                     /locus_tag="VXA80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV0352845.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(39722..40630)
                     /locus_tag="VXA80_000290"
     CDS             complement(39722..40630)
                     /locus_tag="VXA80_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0352846.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(40687..41247)
                     /locus_tag="VXA80_000291"
     CDS             complement(40687..41247)
                     /locus_tag="VXA80_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV0352847.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
BASE COUNT        11570 a         9445 c         8007 g        12305 t
ORIGIN      
        1 ggtaccataa ggccacctcc tacaaataga aaccgatata aatcaatgcc ttccaccctt
       61 agacttccct agttcctgtc tcaagcgaaa catttctttg aaacaggaat aagttaacca
      121 attcatacca atagctagca gaataaaaag aaaccaaatg ccccataact tgatatctgt
      181 cacatttctc aagacggtat tgaaaaacag aactgaaaca actgtccaag caaggctaaa
      241 aagagaatag aaggggatgt aaaaccagta aaaataataa aaaattggaa aaaacttact
      301 atttctgttg gccttctcaa tccagttatc aaaataaaag tacggtgcta aaagtaagaa
      361 tttaaacaaa tgttccatca ccgacatcac cctctttctt ttgatagcgt tttctattat
      421 tttattatat caaaaaaatc cggaactgtc attccagatt ctactttttt atttgcgttt
      481 tcttgcgatg agatgaatcg gtgttccctc aaaaacaaag gccttgcgga tttgattttc
      541 caagaaacgc aggtaagaaa agtgcatgag ttcttcttca ttgacaaaga tgacaaaggt
      601 tggtggtttg gttgccactt gggtcgcata gaaaatcttg agacgttttc ctttgtctgt
      661 cggtgttggg ttgatggcaa tggcatccat gatgacatcg ttcaagacag ctgatggaat
      721 acgtgtattt tgactttcgc tgatttgctt aatcatctca ggaagtttgt ggagacgttg
      781 cttggttaaa gctgatacaa agataatcgg tgcgtaaggc aggtattgga actgctcacg
      841 gatatcttct tcccagtttt tcatagtgtg gttatctttt tcaagcgtat cccacttgtt
      901 gaccacgata atcatccctt taccagcttc atgggcaaat cctgcgatac gcttgtcgta
      961 ctcacgaatg ccttcttccg cattgatgac catcaagacc acatctgaac ggtcaatagc
     1021 acgcatggca cgcataacag agtatttctc agtattttca taaaccttac cagacttacg
     1081 cataccagcc gtatcaatca tggtaaactc ttgaccatct gtatctgtaa agtgggtatc
     1141 aatggcatca cgagttgttc cagcaacagg actagcaata acacggtctt ctcccaagat
     1201 agcattgatc aagcttgatt ttccaacgtt aggacgacca atcaagctaa acttaatgac
     1261 atctggattt tcttcctcat attcatttgg aagattttct acgatcgcat ctagcacatc
     1321 ccctgtaccg attccatgga cagatgagat aggcaatggt tcacccaaac cgagagcata
     1381 gaaatcatat atatcatttc tcatctcagg gttgtccacc ttgttgactg cgaggataac
     1441 tggtttgtgg gtcttataaa gcttacgagc tacgtattcg tctgcatcag taattccttc
     1501 cttaccagac acgacaaaaa cgataacatc tgcttcttcc atggcaattt ctgcctggtg
     1561 cttgatttgt tccatgaaag gagcatcgac atcatcaatt cctcctgtat caatcatgct
     1621 aaaagaacga ttgagccact cacccgttgc ataaatacgg tcacgtgtca ctccttcgac
     1681 atcttctaca atggagattc gctcaccagc gatccgatta aatagggttg atttcccaac
     1741 attgggacgt cctacaatgg caatagttgg tagggccata atttctcact ttctacaata
     1801 atttcttctg ttcaagattt tttctagttg agcttggttc agcttgacca aactgttctg
     1861 ctaggcgctg actccagctt gtggtcgcac gcgccccagc atagtcagcc tgaacacggt
     1921 cataagcttg gattgcctca gttgactgtt cttggtattc ttcctcaaag acaacattct
     1981 ctagtggcag tctcggtttc atatcatgat gttgatttgg cacacccagt gccatcccaa
     2041 agacagaata ggtgtagtca ggtaggttaa agagctctgc cacttcttca gacttgtatc
     2101 gaaccaaacc gataatcaca ccaccatagc ccaagctttc agctgccaac aaggcgtttt
     2161 gtccagcaag agctgcatcg accgaactaa tcaagagacc ttccacacct tggggttgga
     2221 aggtgtcggt atgaagtctg gctccctttt ctgctcggtt caaatctccg acaaagagaa
     2281 ggaaaacagc agactggcga atggcttctt gaggtaccaa ttcatacaag gcatctttct
     2341 tctcttgact tcgtaccaca atcacagagt aggattggaa attcttccaa gatgatgcca
     2401 tctgggctgc tgtcaaaatc tcatttaagt ctacttgggg aatttcttgc tctttaaacc
     2461 tgcgcactga agtatgagcc ttcatcaatt taatggtttc tgtcatcgac ggtttactcc
     2521 ttctaaacga gtctcctcag ccaaataacg gatgcgttcc atgacccgtc tggcttccca
     2581 ggtttcgtca tttccatgtt tcactttcgc aaaatgcttc tccaaatctt caaagttgaa
     2641 gttggatgtg aaaaaggtcg gtaaattttc ctgcatccga tattggagaa tgacctgcag
     2701 gatttcatca cgcacccaag cggtggattg ctcggcgcca atatcatcta aaatcaggac
     2761 ctcagacagc ttaatctcat ccaccaaggt cttaacattg ccatcactga tagcattttt
     2821 gacatcaatg acaaagctag gatagtggag gagagttgat gaaacaccac gtttttctga
     2881 taaatcatga gctaaggccg ccaccatgaa acttttaccc acaccaaagt ctccatataa
     2941 gtaaagacct tttcgaatag ctggatattg ctccacgaag gctagtagct tttcaaaaac
     3001 tggtaagcgc cccaaatcat ccaagtcaac ttgagccaaa ctagctttct tgagactggc
     3061 tggtagattg attaacttga gacggttctt aatagccgct tctttttcag ccgcgattag
     3121 ctcaggagtt tcttcatatg aaacatctgc atagccatga ttcttaacca aaatcggctt
     3181 gtagcctttg gcaatataat ccgtatcccc acggagaaac ttgtcacgct cggtgatgta
     3241 ctgattaaac ttggagatac tgcgatttaa ttcctctgga gtcaaggatt cttgctggat
     3301 aaaagccgca acatcagggt ccttcatgat tttctggacc aaatcttgat aataaaaacg
     3361 gctgggttga cgtttgagta cgtctccgac actttccatc taatctcctc ctttttctaa
     3421 tcgagctaat agttcttgct tcttacgttc tagttccaga cgagtttcct cgctggtttc
     3481 attcttatat tcaggattac tccatttagg aatattggtt tttgctgggg cagtctgatt
     3541 ctgtttttgt gtttttgttt tctgccctcg ctcacggatg cgcaagactg ccttttctgc
     3601 cgaatgaatc ttttgatagg catagtcatt ggctaccttc atggcatatt tctcattgat
     3661 atttgccgaa tcgaccttat taaaggtcaa taaaagaatg atattgatga cttcgtccag
     3721 caagcccaag ccagccatct gttgcaagag ttctctttct gtttgggtaa tggttccctt
     3781 gcgtgtttgc ttgatttctg ccaagaactg cagggcagtt ttacttttgg cttctttgat
     3841 aatggttgtt tccttaagac taaagtcaga ggaaactggt ttttgagcaa ttttttcacg
     3901 catgcgtttg gttgaaataa cctgagaaac agctgttgac ttggccaatt gataggtttc
     3961 aaaccaagtc catttcttct cctcggcaat agcaaagagg tttaagacat cggactgctc
     4021 atccgcaaaa cgaagtccat ctcgagccat cagctggcga aaatgttcca agtcaaaatc
     4081 attggccact ttcttcttga gaccaaggtc ttcttgactg cctagttctg ccaattctgg
     4141 aaagacttga ttgagtgaga caggtatttc ttcaccatca gcactttcaa ctttcaaatc
     4201 ctccacagca gagtcgccaa tctttttctc taagagtctg cgataaacag gatgctccaa
     4261 gaagtcttga ctagatagag gagcatggag ggctagctga taaacatccc ccttttgata
     4321 gagggtcaag agattaaaag cagataagat tttcaatgat tttatcagtc tatccatccc
     4381 aaagttgaga tgattgagga tacttgaaaa aagatattcc tttctaccat tatcccaaaa
     4441 actgattgta taaagataaa ggctcagtgc ctcctgaccg ataatcggga ggtagcactg
     4501 taccagagat gaggtatctt gcgacacccg attattcttt agataagaaa aacggtcaat
     4561 tggcttcatt tatctttcct ttttcttttt agaggactgg gtgatttgtt ggagcaagct
     4621 ctctaactca ctgacatcct taaaactacg atagacacta gcaaaacgta cataggtaat
     4681 ctcgtccaat tcagccaact cctccatgac aagtgaacca atgtcctcac tttgaatttc
     4741 attttcattt cgaccacgga gtttctgttc gatacgattg actaccatgt tgatttcatc
     4801 acttgacaca ggacgtttct gggctgagcg gataatccca ttaaagattt tatctctgga
     4861 gaattgttcc cgtgtgccat cttttttaac aaccactaag gttctttctt ctactcgttc
     4921 gtaggttgta aaacggtgtt ggcactcgtc gcactctcgt cttctacgaa tggtgttccc
     4981 ttcttctgct tggcgactat cgataacact tgacttggta gccccacatt ttggacaacg
     5041 catcctttcc ctccttatcg ttttcttttc attataccat tttttaagcg attcccaaaa
     5101 caattcttct ttttgcttga caagtttttt gttttgttgt attatttaat taagacaaaa
     5161 ggataagaga aaggagacta agatgtcctg gacatttgac aacaaaaaac ccatctattt
     5221 acagattatg gagaaaatca agcttcagat tgtttcccat acactggaac ccaatcaaca
     5281 acttccaacc gtgagggagc tagctagcga ggctggtgtc aatcccaata ccatccaaag
     5341 agccttatca gaccttgaac gagaaggatt tgtctacagc aagcgaacaa ctggacgatt
     5401 tgtgactaag gataaggagc taatcgctca gtcacgcaaa caattatcag aagaagaatt
     5461 ggaacacttc gtttcctcca tgacccattt tggctatgaa aaagaagaac taccaggcgt
     5521 agtcagtgat tatattaaag gagtttaagc ctatgtcatt actagcattt gaaaatgtat
     5581 ccaaatcata tggagcaaca ccagcccttg aaaatgtttc tcttgacatt ccagctggaa
     5641 aaattgtcgg ccttcttggg ccaaacggct caggaaaaac aaccctgatt aaactaatta
     5701 atggcctctt acaaccagat caaggacgtg tcctcatcaa cgacatggac ccaagcccag
     5761 caaccaaggc cgttgtagct tatttgcctg atacgaccta tctcaatgag caaatgaagg
     5821 tcaaagaagc cctaacctac ttcaagacct tctataaaga tttcaatctt gaacgcgctc
     5881 atcatctact tgcagacctg ggcattgatg aaaatagtcg tctcaagaaa ctatcaaaag
     5941 gaaacaaaga aaaggttcaa ctgattttgg ttatgagccg tgatgctcgt ctctatgttt
     6001 tggatgaacc cattggtggg gtggatccag cagcccgtgc ttatatcctc aataccatta
     6061 tcaacaacta ctcaccaact tctaccgttt tgatttctac ccacttgatt tctgatatcg
     6121 agccaatctt ggatgaaatt gtcttcctaa aagacggaaa agtcgtccgt caaggaaatg
     6181 tagatgatat tcgctacgag tcaggtgaat ccattgacca actcttccgt caggaattta
     6241 aggcctaagc aaaggagatt atttatgttt tggaatttag ttcactacga atttaaaaat
     6301 gttaacaagt ggtatttagc cctctacgca gccgtgctag tcctttctgc cctcatcgga
     6361 atacagacac aaggctttaa aaatctacct taccaagaaa gtcaggctac tatgctactt
     6421 tttctagcta cagtctttgg tggcttgatg cttacacttg cgatttcaac cattttcttg
     6481 attattaaac gcttcaaagg tagtgtctac gaccgacaag gctatctgac tttgaccttg
     6541 ccagtttctg aacaccatat catcacagcc aaactaatcg gtgcctttat ctggtcattg
     6601 attagcaccg ctgtattggc tctaagtgct gttattattc tggctttaac agctccagaa
     6661 tggattcctc tttcttatgt gattacattt gtagaaacac atctccctca gatctttctt
     6721 acaggtatat ccttcctact aaataccatt tcaggaatcc tctgcatcta cctggctatt
     6781 tccattggac agcttttcaa tgaataccgt acagcactcg ctgttgcagc ctacattggt
     6841 atccaaatcg tcattggatt tattgaactt ttcttcaatc ttagttctaa tttctatgtc
     6901 aattcactgg taggactcaa tgaccatttc tatatgggag caggtatagc cattgttaaa
     6961 gaactcatat tcatagctat cttttatctc ggaacctact acatcttgag aaataaggtt
     7021 aatttgcttt aaataatttt tacctagata tgtaacatac tcatagaaca aaagagacca
     7081 ggcaaaaagt ctttaaaatt agaaaacgca tagtatcaag tgttaaaaat ccttgatatt
     7141 atgcgttttt tgtgagctga cttatttcct ttcgctatat cgcaaaatga aataagaacg
     7201 gaacgatggg attttggaat tcaaatcaat ttataagaat gttttagaag taatattatc
     7261 ctattccaga ttcagttcac tatacaattg agttttcaag caacctgttt acataatgtg
     7321 tacataatta ggttcgtgat tccacccttt tcacctttaa aaacctcgct ttcgcaaggc
     7381 tcttctattt ataagataag gcacgtttaa aggttttcca aatccctaaa tcatccgttt
     7441 gaagaacgag actagcatac atgcgtccga taaatcctgt tgctaccacc gcaaaaatca
     7501 ctgtaatagc aagtgaaatc catgcttccg ctccctccgc atagccatta atcgttcgaa
     7561 acggcataaa gaaggtcgaa ataaagggaa tataagaacc aatcttcaag aggagattgt
     7621 caccagctgc acctagagct gtcactccaa aaaaaccacc cataatcaaa atcatcaaag
     7681 gcgacaaggc tttccctgag tcctcaggac gagaaaccat agatcctagg aaggctgcca
     7741 agactacgta catgaaaaga ctgatcaaaa taaagagcag ggtattcagt gagatagcat
     7801 ctcccaagtg atccaaaata ccagactgag ccaagaatgg caaatcttta aagagcaaaa
     7861 cggcagccag accacctaca acatagatcc caatatgcgt taaaatcact agaaacagag
     7921 ccatcatccg cgcatagaaa tagtgacttg cccttatgct agaaaagacg acttccataa
     7981 ttttggtgcc tttttcactg gcaacttcct gagctgttac acccgcatag gtaatcagaa
     8041 tcatataaag aaagaatcct aaggcacctg ctgcaattgt ttgaataaac tttttatttt
     8101 ccttggcttc atcaatcttt tctgtgaatt gaattgtctg cgctaagcgt ttttcctgct
     8161 cttgagacaa ggaagcagtt gaacgattaa gctgattttg cagttcattg agtgtacctg
     8221 taacctcaaa tttaattcca ttttcaagcg atgtttcgcc atgataaact gcctttagaa
     8281 cactatcttc ttgatcaatg gtcaaataac cttttaattt ttcttcttta attgcttctt
     8341 tggcacttgc ttcatcttta tagtcgaagt taacaccatt tacattcttc agtccttctg
     8401 ctacagatgg cactgttgtc actactgcca ctttattatt tttagacata gaagaacctt
     8461 ggagatgccc aattcctaca gagattccta aaaagaggaa cggcgaaatc accataaaga
     8521 agaaactcca tgactcgaca tgtcgaagat aggtttcctt gattacaacc cacatatttc
     8581 tcatacttcc actcctgatt ctagtttaaa gatttcatcg atagttggcg cttgttggtc
     8641 aaatgttgcg atatattgac cttgagtcaa gattgggaag agttcccttc cagcgctctc
     8701 atcctccaaa atcaatttcc aactgccttg tttggtcaag ctcacctgtt tgacatgagg
     8761 aagattttcc aattcttcct tgcttcgttc acttgaaaca aagagacgcg ttttcccgta
     8821 ttgattgcgg acatcctgaa ctggtccgtg caagaccaca cggccatctc ggatcatcag
     8881 aatatcgtca caaagttcct caacattggt catgacatgg tcagaaaaga taatggttgc
     8941 tccgcgctct ttttcctgaa aaatgacttg tttgagcaat tctgtattaa ctgggtccaa
     9001 tccactaaaa ggctcatcca agataatcag gtctggttca tgaatcagag taataatgag
     9061 ctgaatcttc tgctgatttc cttttgacag actcttgatt ttatctgtca gctttccttt
     9121 cacttccaac ctcttcatcc attgagggag tttttctttg acttctttgg catccatgcc
     9181 ttttagagtc gccaagtagc gaacttgttc aagaactgtc aatttaggca tgagactgcg
     9241 ttcttcaggc agataaccaa tccgagcata ggtctcctga cgaatatcct gaccatccag
     9301 accgatttct ccctgatatt ctaggaattt caaaatacta tggaaaatcg ttgtttttcc
     9361 agcaccattt tttccgacta gtcccaaaat acgacctggt cgcgcttgaa agtcaatacc
     9421 aaacaaaact tgcttggagc caaaactttt ctctagactt cttacttcta gcatctttca
     9481 cctccgaaat ttcttgcact cattatactc ctttttgata gcctttacaa tgtttttgtc
     9541 catttttaga agactattgc tgtgtaaaat atggcctgga gcacttttat actcaatgaa
     9601 aagcaaagag caaactagga agctagccgt aggctgctca aagtacagct ttgaggttgc
     9661 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
     9721 agtcagtaac tatatctacg gcaaggcgaa gctgacgtgg tttgaagaga ttttcgaaga
     9781 gtattagtga taaatccatt atacagtagc aaacttaatt tataccttcc gctcctcaac
     9841 tgtctatttt taatcctgaa ttgttatttg agtaactcct ttttcctcat aaagttttct
     9901 tcctctaaaa cttctggaaa aaggctaata gtttcagaca acatttttat aagaaacaag
     9961 ttcatctgtc atttcaagaa ggagtaatcc tttatctact aatggacgga acagaattca
    10021 accgcttgtc cgatatgttt tctaaggatt atatagtaaa atgaaataag aacaggacaa
    10081 attgatcagg acagtcaaat tgatttctaa caatgtttta gaagtagatg tatactattc
    10141 tagtttcaat ctgctatatc tattatgcac acccctatag gatctaatga aaatcacaac
    10201 aggctcattc atagatagtt acctcaggaa actaagaaaa cgactacaaa ggaagtcgca
    10261 ttcatcgaaa agtggattaa caactatcct aaaaaatgct tgaactacaa gtcccccaga
    10321 gaagacttct ggatgactaa cttgaacttg aaatttagca ataattaatt caccatctaa
    10381 ctatatttag taattatttc agaactgatt aatattaaaa ttaactaaca attcaaagga
    10441 ttcatactag ccataaatta cgtccatcag agagagactc ttactacttt tagattttag
    10501 tctttctagc ttcagaatac atctaaactt tagggaaaat gactattcga aagcgcgaat
    10561 gcctcaaaat tatctcagat aagctattcg aaacttagaa tgcttttaaa tttatggaat
    10621 tgcgattatt cgaaacctag aatgcatata acctttagtt gacagaccta ttctaagtct
    10681 cgaagggcta tttactttct attccttatc aaaaaagact cattccccct ttctcctcca
    10741 aaatatggta tagtagaaat atactatcta tgaggagttt acatgtcaca ggataaacaa
    10801 atgaaagctg tttctcccct tttgcagcga gttatcaata tctcatcgat tgtcggtggg
    10861 gttgggagtt tgattttctg tatttgggct tatcaggctg ggattttaca atccaaggaa
    10921 accctctctg cctttatcca gcaggcaggc atctggggtc cacctctctt tatcttttta
    10981 cagattttac agactgtcgt ccctatcatt ccaggggcct tgacctcggt ggctggggtc
    11041 tttatctacg ggcacatcat cgggactatc tacaactata tcggcatcgt gattggctgt
    11101 gccattatct tttatctagt gcgcctatac ggagctgcct ttgtccagtc tgtcgtcagc
    11161 aagcgcacct acgacaagta catcggctgg ctagataagg gcaatcgttt tgaccgcttc
    11221 tttattttta tgatgatttg gcccattagc ccagctgact ttctctgtat gctggctgcc
    11281 ctgaccaaga tgagtttcaa gcgctacatg accatcatca ttctgaccaa accctttacc
    11341 ctcgtggttt atacctacgg tctgacctat attattgact ttttctggca aatgctttga
    11401 cacgtaaaaa atccgtttgg tttcccaagt ggatttttaa agcgtagatt aactatagct
    11461 tgatactaaa tatactttgg tatggaaatc atgcatattt ttcgatagtg aagcgaggac
    11521 ttacctagcc tttccaccgt gatagaaaca cctgaaatct aatggtttca ggtgttcgga
    11581 aactttgagc ctagtgtctc aaagtttatg tatggaattt cgaagaaagt cgctaccgtc
    11641 cgtaatcact taaggaaagg ctcaaaaata ttgttttcaa ccacaaaatc cgtttggttt
    11701 cccaagcgga ttttgtgctt tattttgaaa cttcttttgc aagaacaaag ttcccaagtg
    11761 tggcagaacc atttcctgcg actgctggcg tcacgatata gtcacgcaca tctggtactg
    11821 gtaggtaacc attaagaaga gatgtaaatt tctcacggac acggtccagc atatgttgtt
    11881 gagccatgac ccctccacca aagacaatca cgtctgggcg gaaagtcact gtcgcattaa
    11941 ccgcagcttg agcgatatag taggcttgaa catcccaaac agggttgttg agttcaatag
    12001 tttccccacg tacacctgta cgagcttcca aacttggacc agctgcatag ccttccagac
    12061 atcccttatg gaaaggacaa acacccttaa actctttttc aatatccatt gggtgtctag
    12121 caacataata atgacccatt tcagggtgac ccacaccacc gataaactca ccacgttgga
    12181 tgacgcctgc accgatacct gtaccgattg tgtagtaaac caagttttcg atacgaccac
    12241 cagcattgtt acgggcaacc acttcaccgt aagcagagct gtttacgtct gttgtgaagt
    12301 acattggcac gtttagggcg cgacgaaggg caccaagcaa gtccacattt gcccagtttg
    12361 gttttggagt cgtcgtgata aagccataag tttttgagtt tttgtcaata tcaatcggcc
    12421 caaatgaacc aactgcaaga ccagcaaggt tatcgaattt tgagaagaac tcaatggttt
    12481 tatcgattgt ttcgattgga gttgttgttg gaaattgtgt tttttctaca acgttaaagt
    12541 tttcatcacc gacagcacag acaaactttg taccgccagc ttccaagctt ccatataatt
    12601 ttgtcatgat aaacctcttg tttttatttt ctttattata gcatacttcg aaagtctaaa
    12661 tgtctctatt ttttagattt tcctctgtaa atcttactat ctaataaaaa cgaacaaata
    12721 tgtcatttgt tcgttttcac attagagagg attgattaga ttttcacttc gatcacagca
    12781 tcccccttag caactgaacc tgttgcgact ggagctactg aagcgtagtc acctgtattt
    12841 gtaacgataa ccattgttgt atcatcaagt ccagctgcag cgattttgtt tgagtcaaat
    12901 gttccaagaa catcgccagc tttcacctta ttaccttgag caacttttgt ttcaaaaccg
    12961 tcaccgttca tagatacagt atcaatacca acatgaatca aaacttcagc accatttctt
    13021 gttttcaaac caaaagcgtg ccctgttgga aaggcaattg aaacttcagc atcagctggt
    13081 gcatagacca cgccttggct tggtttcaca gcgatacctt gtcccatagc tccacttgag
    13141 aagactgggt cattgacatc agcaagagcg acaacatcac cgacgatagg agttacaagt
    13201 gtttcatttt gaagagctgc tggcgcaact tcttcttttt cttcagccac ttcagcttgt
    13261 tttgcagctg cagttgcgtc tacttcatct tcgtaaccaa acatgtaagt aagagcaaaa
    13321 ccaagggcaa atgatacagc taccataaga aggtattgtg gaagttgtcc gttaccaaca
    13381 taaagcattg taccagggat gatggtgata ccattaccag taccagcaag tccaaggata
    13441 gaagccaatc caccaccgat tgcaccagca atcaatgaaa ggaagaatgg tttacggaag
    13501 cgcaagttca ccccgaagat agcaggctct gtaataccta ggaaggcaga aagagcagcc
    13561 gggaaagcaa gtgttttcag ttttggattt tttgttttaa caccaaccgc aacagtagca
    13621 gcaccttgag ctgtcatagc agctgtgatg atagcgttga atgggttagc atggtcagca
    13681 gcaagtaatt gcacttcaag caagttgaag atgtggtgca cacctgacac gacgatcaat
    13741 tggtgaaccc caccaatcaa gaaaccacca agaccaaatg gcatgctaag aatcgctttt
    13801 gtagcaataa ggatgtagtt ttcaacaacg tggaaaactg gtccaatgac aaagagtcca
    13861 aggatagaca tgaccaaaag tgtcacgaat ggtgttacca agaggtcaat gacatctgga
    13921 acaacctcgc ggacagcttt ttcaaattta gctccgacaa ccccgatgat gaaggctgga
    13981 agaacggaac cttgcaaacc aacaacaggg atgaaaccaa agaagttcat cgctgttact
    14041 tcaccacctt gagcaactgc ccaagcgttt ggaagtgagc cagagacaag catcatacca
    14101 agaacgatac caacggcagg atttccacca aatacacgga aggttgacca cacaaccaaa
    14161 cctggcaaga tgatgaaggc tgtatctgtc aagatttgtg tgtaagttgc aaagtcacct
    14221 ggaagtggca tttcaagagc gttgaaaaga ccacgcacac ccatgaagag acctgtcgct
    14281 acgataactg ggatgattgg aacgaaaaca tcaccaaaag tacggatagc acgttggaac
    14341 cagttccctt gtttagcaac ttctgctttc atgtcatcct tagatgatgt tggtaatcca
    14401 agtacaacaa cttcatcgta cattttgtta actgtacctg taccaaagat aatttggtat
    14461 tgccctgagt taaagaaagc accttgaact ttttccaagt tctcaatcac ttctttattg
    14521 attttctctt catctttgac catgacacgt agacgtgtcg cacagtgggc aacactattg
    14581 acattttcac gtccgcccaa ggcatcgatg actttttttg caatttcctg attgttcatt
    14641 tgcaaaaatc tccttatata acattttgtt cttgtttgaa agcgatttta ttcgccctta
    14701 cgactattat tttatcatgt tttaaaaata tgtcaagcgt tttgcagaaa aatattctat
    14761 ccacttagtg ggcaagcttt agattgattg tttttgactg tttcatcagg aattccttga
    14821 aaaataagtt tgaaaccttg gttaaaaagt cttttatttc attatctttc acattttcgt
    14881 aaaaatttga ttgatttctg tatttatttt atgataatcg tttgatagtt tttcctcttt
    14941 tttaacataa agggcttgca ttttttatcg aaaacagtta ctattaaaag tgataagatt
    15001 tttggaggaa tgaatggaat ggacaactga gcgtcgttac agactttatc aagattggac
    15061 gcaagaagaa attcaacata taaaggaaaa tatggcacaa tctccatggc atactcatta
    15121 ccatgttgag ccaaaaacag gacttctcaa cgacccaaat ggcttttctt actttgatgg
    15181 caagtggatc ctcttttacc agaattttcc ttttggtgca gcccacggtt taaaatcttg
    15241 ggcacagcta gaaagtgatg atttgattca ctttaaagaa actggaatca aagttttacc
    15301 agatactcca ttagatagtc acggtgccta ctctggttct gccatgcaat ttggcgataa
    15361 cttattccta ttttatacag gaaatgttcg cgatgaaaac tggatccgtc acccatacca
    15421 gatcggtgct ttgatggaca aggagggtaa gattacaaag attgacaaga tcttgattga
    15481 ccagccagca gactctactg accacttccg cgatccacaa atttttaact ttaaaggtca
    15541 atactatgct gtcgtcggtg gacaggactt ggagaaaaaa ggcttcgtcc gtctctacaa
    15601 ggctgtcaat aacgactaca caaactggca agcagttggc gaccttgact ttgctaacga
    15661 ccgtactgcc tacatgatgg aatgtcctaa tctggtcttt gtagaggaac agcctgtcct
    15721 tctctactgt ccacaaggat tggataagaa agttctagac tacgataata tctttccaaa
    15781 tatgtataag attggggctt cctttgaccc taaaaatgcc aaaatggtag atgtgtctca
    15841 acttcaaaac atggattacg gtttcgaagc ctatgcaact caagccttca acgctcctga
    15901 tgggcgtgct ctagcagtta gctggcttgg tttgccagat gtttcttacc catctgaccg
    15961 ttttgaccac caaggaacct tctctttggt caaggaactc actatcaaag acgacaagct
    16021 ctaccagtat ccagtcgctg ctattaagga ccttcgtact tctgaagaag ccttctcaaa
    16081 ccgttcccaa accaagaaca cttacgaact tgaactcaac ttggaagcta atagccagag
    16141 cgagattgtc ttacttgctg ataaagaagg taagggactt tcaatcaact ttgaccttgt
    16201 aaacggtcaa gtgacagtgg atcgtagcca ggctggagag cagtatgccc aagaatttgg
    16261 gataactcgt tcttgcccta tcgagaatca ggctactact gctacaatct tcatcgataa
    16321 ctctgtcttt gaaattttca tcaataaagg agaaaaagta ttttctggtc gtgtcttccc
    16381 acatgcggac caaaatggta tcctgatcaa atctggaaac ccaactggaa cttactatga
    16441 attagattat ggtcgcaaaa ctaactgatg tcgccaaact tgcaggcgtc agtcctacta
    16501 ccgtttctcg ggttatcaat aaaaaaggat atctatctga gaaaaccatc caaaaagtca
    16561 atgaagccat gagagaatta ggctataaac ccaacaacct agctcgtagt ctgcaaggaa
    16621 aatcagctaa gttaatcggc ttgattttcc ccaatatttc caatgttttc tatgcagaat
    16681 tgattgataa attggaacac caactcttca aaaatggtta caagaccatc atctgcaaca
    16741 gtgaacatga ttctgagaag gaacgcgaat acatcgaaat gttggaagcc aatcaggtgg
    16801 acggcatcat ttctggtagt cacaacctag gaatcgaaga ctacaatcgt gtgacagcgc
    16861 cgattatttc ctttgaccga aacctatcgc cagacatccc tgtcgtctcc tctgacaact
    16921 atgctggtgg ggttcttgct gcccaaacct tggtcaagac aggtgcccag tctatcatca
    16981 tgattacagg gaatgacaat tctaattcgc caaccggact gcgccacgct ggttttgcat
    17041 ccgtactccc aaaagctcct attatcaatg tttccagtga cttttctccc gtcagaaaag
    17101 aaatggaaat caagaatatc ttgacccggg aaaaaccaga tgccattttt gcttcggatg
    17161 atttgacagc tattctggtc attaaaatcg ctcaagaatt gggcatttct gtcccaaaag
    17221 agctcaaggt catcggctat gatgggacct actttatcga aaattactac cctcaattgg
    17281 ctactatcaa gcaacctttg gaagagattg cttgtctcac tattgatctt ctcttgcaaa
    17341 agattgaagg caaggaagtc gccacaactg gttacttctt accagttaca ctattaccag
    17401 gaaaaagtat ttaaacacaa gaaaactcag accgattcgt ctgagttttt tatgatctta
    17461 aattttcgag atagcgctgg gctgtctcta ggttaaaggt tttatctgcg ataagctgct
    17521 cgactagggg agcaacttca gattcactag ccccagctag gagagctagg gatttggcct
    17581 gtagtttcat gtggccttgc tggatgcccg tacttaccaa ggctttgagg gctgcaaaat
    17641 tttgagcaag accgatggac acgataatct gtgctaattc tctggcagaa ggatttccta
    17701 gtagatcatg actgagagct acacgtgggt tgaggccgat agagccaccc ttagtcgcta
    17761 caggcatggg cagggtcatc tcaccgacca attcttctct ttcaaggtcc agcgtccagc
    17821 aactaagacc ttgataacgt ccatctcgac tggcaaaggc atgggcccca gcttcgatgg
    17881 cacgccagtc attaccagtg gcaatcaaaa tcgcatcaat accattaaaa attcctttat
    17941 tatgagtagc agctcggtaa ggatcagcct gcgcaaactg actagccaac gcaattttct
    18001 ccgcaatctc tcgtccttga tccttttggc ggctcaagta gcgaaaggcg atgcgacagc
    18061 ttgcagtcac cagagaatca gtcgcgtagt tggacaggat tcccatgaga ctctgtccct
    18121 gactgagttc ttctaagact ggtttcaagg cttccagcat ggtgttgagc atattggcac
    18181 ccatggcttc ctgggtatca acatgaatat aaacaacgag aaagtctggt tcacctttta
    18241 tctgctcgac atgcaggtca ctcgccccac ccccacgttt aacgatagaa ggataggctt
    18301 gattggcaag ctccaagagc tccgctttct tgctggcaat cttctcttgc gctagtttag
    18361 gattagcaat ttgataaagg gctacctgcc caatcatctg gcgctgatgg acttgtgcag
    18421 taaaaccacc tgcacgcttg atgattttgc tggcatagct ggccgccgca accacagagg
    18481 gttcttctgt cacataggga acggtgtatt cctgaccgtt gacaagtacc tccggaacca
    18541 gcgaataagg cagagaaaaa gttcccacca cattctcact cagctggtct gccacagtca
    18601 cactcatctg ttcatccttc tccagactag cttgtctctc aggactaagg agcgcctgag
    18661 cttttagcag ctcgaggcgc tcttggtatg attttttaga aaatccattc caacttatct
    18721 tcattatttt tcaaccttgc tataacggcg ttggtggtca agaatttcaa ccaaggcaaa
    18781 atcttgattt tcatagccag caaactgggc agagtttgtt tcatccaagt ttacttcctc
    18841 aaaaaagacc ttttcatagt ctgcaacgga tagggcagtt cgttggttga gcttgttcaa
    18901 gcggtcttta tccaaataag cttcatatcc ttcaaccaat tcaccactga agaactcagc
    18961 cacagctcca cttccgtaac tataaagggc gattttatcc ccagctttca agctatctgt
    19021 attttccaag agagacaaaa gtccaaggaa aagtgaacct gtgtagatat tccccacctt
    19081 ttgactgtag agaatagact ggtcaaaatg cttttgtaag aggtcttttt tctcttgagg
    19141 caggctctta tccatgattt ttttcaagcc ttttagcgct aatttaggat aaggcaagtg
    19201 gaaacaaaca gccgcaaaat catccagagt aagctggtag cgtttttgat attcaagcca
    19261 agtcgttttc aaactatcca agtattgttg ggtagaataa acaccattta cataaggagt
    19321 tgtcgagtaa tttggtcgcc agaaatccat gatgtcacgg gtctgagcta cattgtcatt
    19381 attaaaggcc atcatgcgtg gattttgtgt aatcaacata gctacacttc cagcaccttg
    19441 agttggttct cctggagttt caataccgta tttggcaata tcactggcaa tgaccaagac
    19501 cttggactcc ggagaatttt ccacatgcaa tttggcataa tgaagggcag cagtcgctcc
    19561 ataacaggct tctttaatct cgaaactacg agcaaagggc tggatgccca gcaagccatg
    19621 cacaaagacg gccgcagcct tactctggtc aattcctgac tcggtagcca caatgaccat
    19681 gtcaacttct tgtctttctt gctcagttaa aatagagtca ctagcactgg ccgccaaggt
    19741 cacaatgtcc tcagttaggg acgcaatact caattccttg agtaagagtc ctttacttaa
    19801 tttttcaggg tcaattcccc tcgcttctgc taagtcttgt aatttcaaga catattgact
    19861 ggtcgcaaaa ccaatcttat caataccgat tgtcatattt acctctgttt tatcattcat
    19921 gtaaaaaatc gttctatact attttatcac aaatggcagt aaaagagaga aaaaagactt
    19981 gattcaccaa atcaagcctc ttattggtca tcattttaaa gaatgattag ttgctagaga
    20041 gttcaccgat ataagtagct ttataagctc cattcacagt tatcagctcc tggaggatca
    20101 aatttcctga gtaagtcctt cccatctcat ctacaaattt ttgataaaac tgactggtcg
    20161 gaatttctct gacatcctta tcaaatgtct tatcaagtgt tttactaacc ttctcagcaa
    20221 tcaattgatg ctcttgccat ccactttgaa actctgagcc cgaactagaa accatgactg
    20281 ggataaacaa caaggtcagt agacttacag acaataagga aaatagtaga cttcctgcaa
    20341 aactagaatc ctagttcatg attgataata ccagcaatca aattcattcg taatccgaag
    20401 cgtttacgat gatttcgata ggttgttgaa aatattttaa acgtttttac tttggcaaag
    20461 atgttctcaa ccttgcttct ctccttagat agcgcatggt tataggcttt atcttcagct
    20521 attagcggct tgagtttgct ggatttacgt ggagtttgtg cttgaggata tatcttcatg
    20581 ggcccttgat aaccactgtc agccaagatt ttaccagctt gtccgatatt tctgcgactc
    20641 attttgaaca acttcatatc atgacaatag ttcacagtga tatccaaaga aacaattctc
    20701 ccttgacttg tgacaaccgc ttgagccttc atagcgtggc atttcttttt accagaatca
    20761 ttcgctaatt ctttttttag ggcgattgat ttgtacttcc gtcgcatcaa tcattaccgt
    20821 gtcctcagaa ctgagaggag ttcttgaaat cgtaacacca ctttgaacaa gagttacttc
    20881 aacccattgg ctccgacgga gtaagttgct ttcgtgaata ccaagatcag ccgcaatttc
    20941 ttcataagtg cggtattctc gcacatatag aaagcgttgt taatttattt atctcatttt
    21001 tcagaaaatt cttttatttc tgtaaagtct acgatactcg atgtgtcttt atataatgat
    21061 acagtctgag aatcactgtt ccgctagcca ttccaataga gattaccaga gccaacatga
    21121 caaccaaggt cgcacttgcc agtgctttat tatagtcccc tgtcacaaaa aaggcagttg
    21181 ttcggtagga gagataacct ggaaccagcg gtgccaaaat ggccaagata aagaccacag
    21241 caggtgtctt ataaagaata cttaaaatct ggctgacaca agaaccaata atggctgcaa
    21301 tgaaggtagc tacaatgaca ttggtcggtt ccttgagcaa gagatagatt agccagacag
    21361 tcatgcccaa aatccctcca ggtaagagca tagaccgttg cacattgagt acgattaaaa
    21421 aagtgataat ggcaagaaaa cttgctactg cttgtaataa aaaggttgtt agtgtcatat
    21481 tagttcatca ataccaaggc gacagaagtt cctgccccta aagcgagggt aatgagcagg
    21541 gattcaaaca tcttactcat accagagttt atgtggttgg tcataatatc acggaccgca
    21601 ttggtcaagg caatacctgg tacaaacggc atgaccgcac cagctataat caaatctgcc
    21661 gttgaaggaa aacctgtgta gcgagcccaa aactgggcaa ttatcccaaa gacaaaggct
    21721 ccagcaaagg ctgtcacaaa gggaattcgg ataaattttt ccacatagag ggaaaaggca
    21781 aaaccaaata aggtcgccac tcctgcccca agtgcgtcgt agatatttcc gctaaacata
    21841 actgaaaaga aaggagcact aaaggtcgca gccagagtta cctgcaactt agtataggga
    21901 aggggttgag cttgcaaggc cgtcaattgc ttaaaggctg tttctaagtc aatctgcccc
    21961 ccaactagct gacgagaaat ctggttcaca tcgcagactt tttcgatgtt ataagaagag
    22021 gaggtcacgc gcttcatgcg cgaaatattg gtattttcaa tagagaaaaa gatagcggca
    22081 ggcatggcaa ggacattgca atccacaatc ccctgcgaat gcgcgattcg aatcatggta
    22141 tcttctacac gatggatttc tgagccactt ttaaggagaa tagtccccgc tagcataatc
    22201 acatcaatga cggcatttaa ttcttttgat tcttccatgc tttcctcctt ttatcaactc
    22261 cctctattct atcacaaatc tggactcaaa aaaaatcttt gccatgaaat catgacaaag
    22321 attgattact cattttgatt atccatctac ttttaaggag tagctgaagt tgttttaggt
    22381 ttgtagattg aaatcttgac tctagtctta ttgaggtcta ccttttcacc tgctctagga
    22441 ctttgttcaa caaccatgcc ttctgcacta cctgcaggcg ctgtcgtcac ttctacaact
    22501 tctatattag cttccttaat cccaacaatt tgaatcaaat tgttcttagt aaactccaag
    22561 ctggaaccaa tgtaactcgg catggcaaca cttgtaactt ttttagctac tgtcaagaca
    22621 atttgagtag gtttactcac atcataagtc gttccggcac ctggactttg tttcataatc
    22681 gttcctggtt cgctttcgct ggactcttct tcctctatct taatcaaatt ctcaggaacc
    22741 ttcttctgct tgagttctga gattacttct gtagagttcc gtccaatata gttccctaat
    22801 tgaatcgtcg tagctttttt agctactgtc aaaataattt gagttgcctt gctcaagtca
    22861 taggtcgtac cttctggtag actttgcttc aggaccgttc cagcctcact ctcattcgac
    22921 tcttcttcct caattttaat caaattatct ggaacttttt tctcttttaa ttccgcgata
    22981 acatcagaag atttccggcc gacataatta ctaatttgga aggattgttt gcctgatgag
    23041 acaaccagat tgattttcgt tccttctttt cgaccagttc cagcgccagg atctgtacgg
    23101 ataatccgcc cttcttccac cttttcacta gcctctgtct tctcctcacc aatctcaaaa
    23161 ttggcttttt tgagcgttgc cttggcctct gcaactgtct gacctgccac atctggaatg
    23221 gcaatggttg caggagttct ggatagtatc caaataagag aagctgccac caatacaagg
    23281 ctggccaaca aaatcaggta acgcatctta aatctatgtt ttttcggtgc ttgtggttgg
    23341 taagtttcct ctgtcacagc ctggcttggg tttttgattg atttgtgttc tgtctgcgct
    23401 tgaaccttag gaatagatgt caaggtactc tgagaaacct tcggcaaggt cttggtatct
    23461 gccttgctcg tttcatcaaa gattaactta ctttcatttc tacgattgta ggacaagcta
    23521 ctagacaagt ccacatacat ctctgaaacc gagcggtagc gattggtcaa ctttttagca
    23581 gttgccttga taataacatt ttctaaagcc tgaggtacag atggattttc tgcaataacg
    23641 gacggcaggg gtttctggaa atgctggagg gcaatggtca ccgcgctatc cccgtcataa
    23701 gggatatggc ctgtcaacat ctcatagaaa ataatcccca tggcatagat atcactctgc
    23761 acagtcgcct tagaaccacg cgcctgctct ggtgacaagt aatgaactga gcccaacatc
    23821 gagttagtct gggtcagact tgtctctgca aaggctacag caatcccaaa gtctgtgacc
    23881 ttggccgtcc catctggtgt caaaaggata ttttgaggtt tcaagtccct gtgaacaatt
    23941 cctcgagtat gggccaagcg catagccaag agaatttgtc ccatgatacg gactgcttct
    24001 tcattagaaa gaggataatg ttccttgata tagcgtttga ggtctagtcc agcaacatac
    24061 tccattgcaa gatactgttg accgtcttcc tcaccaatat ctgttatccg aacgatatga
    24121 ggatggtcta gatctgccat agctctcgct tcacgctgaa aacgagctac agctatcggg
    24181 tccgtctggt agttggtcct cagaaccttc actgccactt cttccccatc taagattaag
    24241 tctttggcta ggtagacatc cgccatacct cctcgaccaa tctgtttgac aatccgatag
    24301 cgtccggcaa aaatcttgcc gatttggatc attctgcatc ctcctcgttc atagaaacaa
    24361 gggcaaccgt aatgttgtct aaacctcctg cattgttagc aaaacgaaca agtgtctccg
    24421 ttttatctgc taaaggaata tcactggtta caatatcacg aatctcactg cctgaaatca
    24481 tgttggtcaa gccgtcacta ttgagcaaga gatagtcacc tgactcaagg ataactgtcc
    24541 caaaatcagg ctgaatttca tctttttgcc caatagactg ggtgataata tttttttgcg
    24601 gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt gagcaattca ttaaccaaag
    24661 aatgatcact cgtcaactga tggtattctt ctccacgaat caagccgata cgcgaatcac
    24721 caatatgagc atagatagcc tgattatcaa taatagcaag gacttccaaa gtagttccca
    24781 tgcctctgta agcttcatcc tgaccaagct ggtgaatctt ttgattttca atttctaggt
    24841 aatgggcgaa ccattcacgc acttcattga ctgtatcgat ctgggtatca acccaagcta
    24901 cacccaggtc tgtgaccgcc atttcactag cgatattccc tgcgcgatga cctcccatcc
    24961 catcagctaa aataatcatg gtacgtccag ctctattgac atagtggttg acatagtctt
    25021 ggttatttgt tcgtttctga ccaacatctg ttaataatga aatttccatg tgtcagttcc
    25081 ttcctaatcc gatatcttgc gaaattgact gatgaagaat ccatcacttc catacaattc
    25141 aggtgtaatg aggatacagc cgtctttcat gatatcctta cattcatgtt ctagttttac
    25201 ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg acttgaaaat tctcctctga
    25261 gacgatagtg caggtgctat aagttattat accacctttg cctagtattt gacaaacact
    25321 acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa tctgccgttt ctttattgta
    25381 tttgatatct ggttttcggc gcaaaagacc gattcctgaa caaggagcat ccaccaaaat
    25441 cttatcaaag gaatcctggt caaaaaactc atgcaccttt ctggcatcca atttttgagt
    25501 ttgaacccga tctgcaactc ccagacgttg ggcattttct tgaattaaat ccaacttgtg
    25561 gtcgtacaag tccagagcag taacctgacc tgtcgtaaga taagaggcta tatgggctgt
    25621 tttcccacct ggagccgcac aggcatcaag cactcgctca tcaccttgta aatcaagcgt
    25681 cggagcaacc agctgactgg actcatcttg gatggtaatg gctccattcg caaacaaatt
    25741 atgccctgca aaatgcccct gctccttaac cagaccagtg gttgctaaaa gggaattatt
    25801 cgcctccaac aaggcttgga tttcctcttt tcgacttagg tctgttacac gaatactggc
    25861 tttgtttcgc actaacaggc tttcaaagat ggcttttgct ctctcctctc cgtattcttc
    25921 cttgagtttg gcaactagcc aaactgggag agaataggca atggagtcac gcttgttttt
    25981 tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag atacggcgaa ggacagcgtt
    26041 gaccaatttt tcactgcctt ttttacggag tttggccaat tccactgctt cattgaccac
    26101 agcatgatct ggaatcttgt ccaaatagcg gagttggtag gcactcatga gaagaaggac
    26161 atagagccag ctgtctaact ggtctctgtc ttcgataaag tgggataggt accattccag
    26221 agtcagttta agggctaccg ttccatagac tagctcggtc actaagccct tgtctgctgc
    26281 agagagctga ctccccttga ggtgtttatt taaggcaata tttgaatatg cttgattcac
    26341 aaaaacatcc tctagcactg ctagagctaa acttctagcc gtttctactt tagtcaccaa
    26401 atcgttctcc tacagtcaat gtacgtccaa ctccgttgag gaaggaagca atgtccatct
    26461 taggcttacc agctggctgc acttgtttga gggatagagc cccttcagcc gttgcgacaa
    26521 tcaattcttt cttgcagata gagagaatct cacctggatt tccctgacct tctactggta
    26581 gggcttcata aatcttaaag cggtcgccct taaggaaagt atgggcaaca ggccaggggt
    26641 tcattccacg aatttggtta aagagttgac gattggtttt gttccagtcc agtttttctt
    26701 cctctggctt tatatttgga gagaaggtaa cctgactcgt atcctgcggt tcaggtttga
    26761 tatcaccagc aatataggca ggcagagtgt ccaaaagcaa atcacgacca actagcgcca
    26821 atttttcaaa caaggtgcca acattgtcct catctgtgat cggaatgctg cgacgagaaa
    26881 tcatatctcc tgcatccatt tccttaacca tttccatgat ggtcacacca gcttcctcat
    26941 ccccttgaat caaggcataa tggataggcg caccaccacg gtgtctagga aggagggagg
    27001 catgaacgtt gacagcaaag tccatgctat caaggagttt gcttgggaga aactgcccaa
    27061 aagcagcagt cacaattcca tctgctccta gcttcataag ttcttccatc tctggacttc
    27121 cagataattt ttcaggttgg tagatagata gtcctgcttc cttggcagcc tgcttgactg
    27181 gggtttcttg gataactttt ttacgaccaa cagcacggtc tggctgggtc acaacggcta
    27241 gaatttcgta acggtcatct gtcaaaagtc cttttaagac tgttgctgaa aagtcggggg
    27301 tccccataaa gattagtttt gtcatatctt ctccttctta taaaaattgc tgcggctcat
    27361 ggtcaatgct gagacggagc tcactatttt cccgttcttg agtcaaggct aaaacctggt
    27421 tgagggtcgg ccccagctca tcttctaaac ggtatttaat taaaatctgg taatgataga
    27481 ggttgtgggt acgggcaatc ggttttggcg ttggccccag aatgggactg gtctctgaca
    27541 agcctgaccg caaaatgttc atgacttcat aggcacgttt gaaaacctct tcttctttct
    27601 tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg atagccgagt tgtcgtctga
    27661 ttcccatttc ataggcataa aagccttcgt aatcttgatc cttggcaaat cgaatagcat
    27721 agtgctgcga attgtaggac tgtatcaaga cttgacctgc tttttcagcc cgacctgctc
    27781 gacctgccac ctgagtcaag agctggaagg ttctctcaga agaacggaaa tcaggcagat
    27841 tcaaggccgt atccgcattt agaactccga ctagggtaac attgggaaaa tccaaaccct
    27901 ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc tcgcccaaac tggtcaagca
    27961 aggcttggtg actgcctttc tttcgagtcg tatccacatc catcctcaaa atgcgagctt
    28021 ggggaaagag ttctgctagc tcatcataag ccttctgagt tcccgtccca tagtaacgaa
    28081 tactgcggct cttacagtta ggacagacct gaggaatatc cttcgagaaa ccacaataat
    28141 ggcagttcat agtcttggta tccatatgca aggtcagaga aatatcgcag ttgggacaag
    28201 tatccaccgt cccacactcc cgacacatga caaagctaga ataaccacgg cgattgagca
    28261 tgagaaccac ctgctctttt ttaaccagac ggtcttggat agcctctagc aaaggaggcg
    28321 taaagtttga cgtctcattt tgtccgatat agtctcgaaa gtcaatcact tgaacctcag
    28381 ggattgtagc caaaggattg gcacgttggg ttagacgtaa gtgttgatag acgcctttgc
    28441 cagcacgtgc ccggctctct aagctcggcg ttgcagatcc aagtaccaga gttgcttgat
    28501 tatactgagc ccgtaaaata gctacctctc tggcatggta acggggattg ctgtcctgct
    28561 tataagccgc ttcatgctct tcatcaataa tcatgacacc cagatttttc agaggagcaa
    28621 agatagcaga tctggcacca acaacaactt gggcatcgcc acgctccacc ttgcgccatt
    28681 catcatactt ttcaccattg gataatcctg agtgaagaat ggctaccttg tccccaaaac
    28741 gtgctataaa acgctcggtc atctgaggag tcaaggaaat ctcaggtacc agcaaaatag
    28801 ctgtcttgcc cttatccagg gcaccttgga taatctgcaa gtaaacctcg gtcttcccac
    28861 ttcctgtaat cccttgaagt agaaagggag gttgagaact gccaatagaa ctcacaaccg
    28921 catcacgcgc ctgtctttgt tctggattta actccaaagg tctacttgct tcaattcctt
    28981 caaaataagc agccgagcgt tgaacttcct tttggactat ggtaacagca ccttgatcca
    29041 caaagaagtt gacttgctct cgcgagtagg actctaacaa gctagccaag gaagcgctct
    29101 ctggatgaga cagcagataa tctctcagtt ccaacttttt cttggcacgt gtagaaatct
    29161 caacaccttc taattgggca tggtcaacct cataccaaga ctgggtcttg accttctttt
    29221 gatcgactgc ctgatattcc agaccaagca ggccttttct agtcaaacgc atcatttcag
    29281 cttgcttggc aaggtctagt gaagaaaagg ctagcgaatc ttctgaacca aacaggcgca
    29341 ctcgttcttc ctgactcaag ccttccagag gatagagaat cttgtcatag ctagaattca
    29401 gaaaccctgg aagcatggcc ttgaggatag agattttgta ggagaagaca gatttgcgta
    29461 actcctcagc cagccagagt tgttctggcg tgagaacagg agaaaaatcc agcacctctg
    29521 caatatcttt taaatcttgc tccatctctt ctccatctga ttgggacttc aaaccaagaa
    29581 caatcccttg aatcaggcga ttacccttac caaaaggcac atgaacccgc atcccaactt
    29641 ccagcattcc ctcaaattcc tccggaatcc tgtaactata gggctggtcc gtctgcatca
    29701 agggcacatc tacgataatc ttagctaggg ccatcttctc acctcctcct tgtcagcaca
    29761 ttcttgcaat agaaaaaata agattgagtc cccccaacct taaatttttt caccatcttc
    29821 tttttcttta gcaatttgct ctttgatttt cttttcttct tcttctttgc ggcgtttttc
    29881 ttcttcgata cggcgacgca ctgcttcacg ttttccttct ggatctgggt gaattgtaac
    29941 gtttcctgat tcgatttctt ctaaagcgcg aagagttgat ttttcagact tgaaaccttg
    30001 agttgctggg gcacctgctt ccaattcgtg ggcacgtttt gcttccaaga ttacgagtga
    30061 atattttgaa ggaaccttgt cgagcaaggt atcaatagag ggttttaaca tcatttgctt
    30121 gtacctattt tctaaatttt atcgggtagt tggagatttt ggtaacatct cctgatagtg
    30181 accaatgaca cgatccacac agaagtgttc tgcttcaatc acacatttga cacgttcagc
    30241 agctaggggc acctgatcgt tgacaatcgc ataatcatac tcacgcatga gggcaatttc
    30301 ttccttggcc ttttcgattc gttgggcaat cacttctgca ctatctgttc cacgacctac
    30361 caagcgatct tgcaattcat ccaaatctgg tggtgtcagg aagataaaga cagcatctgg
    30421 aacctttttc ttgacctgaa gagcaccctg aacttcaatt tcaaggaaaa catcgattcc
    30481 cttgtccaag gtttcattga cataggtcag aggagttcca tagtagttac cgacatattc
    30541 tgcgtattcc aacatctgtc cttgacgaat cagctcttca aattcttcac gagtacggaa
    30601 gaaatagtca acaccgtcca cttctccagg acgttgtgcg cgtgtcgtca tcgatacaga
    30661 atattgaaat tggttttcag aactctcaaa aatctctctt ctaaccgttc cttttccaac
    30721 ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct gccattgtgt ctccttttag
    30781 tcaatctgtg aaataacatt tctctagaat aatggcaaaa agccagatta tcctttacag
    30841 tctttctatc tagtgtaaca aaaaagcagt aatttttcaa ctgctctttc ttatttattt
    30901 agcataatct actgcacgaa gctcgcgaat cacggttacc ttgatatttc ctggataatc
    30961 gagattgttt tcaattttct tacgaacttt gtgagccaag attgtgactt tgtcgtcctt
    31021 gatttttcct ggattgacca tgatacgaat ttcacgtcct gcttgaaggg caaagctagt
    31081 ttgcactcct tcaaagccgt tagcaatttc ttccaaatca tggagacgct tgatgtagct
    31141 ttcaagagac tcactacgag cacctggacg ggctgcgctc aaggcatctg ctgcagcgac
    31201 aataactgct atcacgctct cagcttcaac atctccgtgg tgactagcaa tcgtattcac
    31261 cacaactggg ggttccttgt acttacgggc caattccata ccgatttcaa cgtggctacc
    31321 ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg aatccagcac gacgggcaag
    31381 agccgcattt tcaccaagtt cgctcgccat gataccagcc aacttagcaa cctcaatcga
    31441 atggcgcaaa acattttgtc catatgaagt acggaactgc aaacgtccca taatcttcat
    31501 caagtctgga tgaaggtttg gcgcaccaat ttcataggca gcagcctcac cgtattcacg
    31561 aatcttattg tcaatctctt gacggttttt ctcaaccaac tcttcgatac gagctggatg
    31621 tatacgacca tctttgagca acatttccat agtcatacgg gcaatctcac gacgaatcgg
    31681 atcaaatcct gacaaggtca ccacttctgg tgtatcgtcg ataatcacat cgacccctgt
    31741 caaactttca aaggtacgaa tgttacgacc ttcacgacca ataatgcgtc ccttcatagt
    31801 atcgtctggc agatgaactg ttgagtttgt tgactccgct acatattcac cagcgatacg
    31861 ttgcatagct tgaaccaaga tgtccttggc cattttgtca gaacgttcct tgacctcttg
    31921 ctcagcttcg cgaatgcgac tggcaatctc cctggtcaag ttttcctctg tctgagccaa
    31981 gataatatct cgtgcttctg cctgagacag agcaccaata cgctctagtt ctgcttcttt
    32041 ttgtctttcg acttcctcta attgctcttc acgcgcatca aggtttttcg ctctatcaga
    32101 aatactttgt tctttttgtt caagtgtttg ttctttactc gtcaaattgt cgtccttacg
    32161 gtcaaggcta gtagctctct ctgtcaaacg actttcgatt tgtttgagtt cttgacgttc
    32221 tgatttgaat tcagcgtcca cttcttcacg gtattttctg gcttcttctt tggcctccaa
    32281 tagtgcttct tttttaagag acttgctttc acgtttggct tcattaacaa gtaaatccgc
    32341 ttcacgctca gcttgtccac gtaaattagt tgcttcttgt tcagcattta aaagcatcaa
    32401 ctctgcagct tcctgagatg atttcatctt agctgagatg ctgacatatc caatgactaa
    32461 accaatgatg acggcaaaaa cagcaatcgc aagcgacatg atttccatgt ttttacctca
    32521 ttttattgtt attccgaatg acatacattc ttttacattc taccataaaa aagtgatttt
    32581 cacaaaccta aaatagaata tgttttgagg aatttggaac acatttacca aaataaactt
    32641 gttgtttaga aatagtagtt tagtagagac ttgagaaaaa gcctaccttt caatagactt
    32701 agtaatgatc tttaaaggac aagaaagcca cgctatctcc atccatcata taaatcaagc
    32761 gattttctgc atcaatacgc cgtgaccagg ctccttggta atcatatttg agtggttctg
    32821 gtttacctat tcctgtaaag ggatcacgtt gaatatcctt gattagttta ttgattcttt
    32881 ttaacgtttt cttatcctga ttttgccagt agcaataatc tgcccaggca tcttctgtaa
    32941 acttgagcag catttcttac tcctcaataa catggacctg agtacttcca gcacgaactt
    33001 gagccattcc tcgcaaaacc ttatcagaaa gttccttatt ttgagcaatt ctcagggttt
    33061 cttggatact atcccactca ctctttgaaa ggactacaat gtcctcatct ggatttttat
    33121 tgaccaccgt caaaggctca aattcatcat ttaccttctt catgtagtcc tttaaatgat
    33181 ttcggaatgt tgagtaaagg actgcttcca taaccatacc tcgttttagc tcttttccac
    33241 tattatacac gaaaagaaag aaattgtcag gaacttgtac aagattttct tttctatcta
    33301 tttatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt
    33361 acggcaaggc gacgttgacg cgatttgaat ttgattttcg aagagtatta ttcgtaaaaa
    33421 atctcaaaaa gcctaccttt cggtagactt agtttgtttc tattctaatc ggcactcttc
    33481 caaaattttg ctctgctata cttggcttcc ctagttggta aatctggtct gctttttggg
    33541 tcatagcatc ccaaggttct ttgccaattc ggctgactag attgacctgt cctttcagag
    33601 acttgagatg ttgcctgcct ttttcagtaa atccaaggac atgaatggct tctggcaaga
    33661 cattttctct agcctgcacc aaaatatagg tcaataggcg tctgacacgc gccttggtgt
    33721 aacgtttggt agtcactaac tcgaccaatt cttccacaga ctgggctgtt ttaatagctt
    33781 ctttaatgcg cacagccatt tcttgattga cctgatagat ggtggttagg tcgggatttg
    33841 acaagatttg atagcggagc aagggaaaat agtcttccca gatcacctta ctggcctgct
    33901 caaatagggc aacagaaggc ataaaacgtt ctaagaaatc ttggtccttc tgatgttgac
    33961 ggagggctgt cgccgaggca aagtccacat ctttattcac agaatggtaa cctgccccct
    34021 gacgctgaat cggatgcagc ttgatatttc gtcctgcaac cgccttggca taggccagag
    34081 caagaacatg attgggggta ttacctgaaa aatcaagacc agcaaattcc ttccacatag
    34141 cttgggtttt ctggggatag gaaagggaat caggcagatt ttccacaaat ttctccatct
    34201 cagcaccttt ctctgtgtat aagtcagcaa ttttctgata atctcgaact tcctctgtcc
    34261 caaaaactag actatcaatg cccaagcgat ccaagatatc catagctcct tgaccaaaga
    34321 aatcagctgc ctgaacactg actaaaaagg gcaattccac taccaagtct gcgccatttt
    34381 ccagcgccat ctgggtcctt gtccacttat ccacaatagc aggctctcca cgctgcatga
    34441 aattccctga catggcaaca attttcagtc cctctgcctg ttccagcagg tatttatggc
    34501 cattatgaaa aggattgaac tccgcgataa tacctgttat ggtcatgcta atctcctact
    34561 tctgcgccac aaaaaaccaa cgggtgctag tttctgttgg ctccttatcc tcaaagtctg
    34621 catagagttt gaaggactta aatccagcct gttccagcaa gatatcatag gtcaagatct
    34681 cataggtcct ctcctcatgt acttcatcgt ggcgactaaa ggaaccgtca gcctccttga
    34741 taaagaaagt cagctcatgc acgatggagt gaggagcttc gtcctcatat gtatcccaaa
    34801 gcatggcaaa atcttccgca ttttcatggt aggaatagcc agggaatact tcatctgtct
    34861 ggtaggtcga gtgcacgtca aagatgaaaa ctccctcttc attcagcgca ttgtacacgt
    34921 ccttaaagac gtcccctact tccacctcat cctgcatata gcagatagag tccgaataac
    34981 acgtgacaaa atcgtatttc cctgccttgg acaaattcag catattacct tctacaaaag
    35041 caatcttttg cttggctgaa gttgctctct tctccgcaat cttcaacata tccgcactca
    35101 agtcaagtcc agtcacatca aaaccagcct gagagaagcg cactgattga attcctgtcc
    35161 cacaagccaa ttccaagagt ttctttctct ccttggtctt aggcaaatga cgcagagaaa
    35221 aattcgtcca tttgtcgtat aaactatcgt ccatgaccgc atcatagacc gccgcaaagg
    35281 tttcataagt cgccataatc atgatactaa gagctccatg gtaaacacca ctagcccttt
    35341 acctttctat caatttttct aaaataagca aagaaaccca gttgactaca actgagttca
    35401 tcttctaagc aagagcttct gaaatatcta ctgaattcgc ctcatgccat agcttctcta
    35461 gattataatg ggcacgcatt tcttctgaaa agatatgcac gacaacagca ccgaggtcca
    35521 gtaagaccca gcctccagct gcatcgcctt cgacatggct acctttaaag cctgcttgag
    35581 ctactttttc acggatatta gctgcgatag cgtccagctg acggctattc attgagctag
    35641 tgatgacaaa gtagtccgtc acactagtca aatcttgtac atcaagtgcg aggatatcct
    35701 ccgcacgttt ctcatcagcc gctttcacga ctagttctag taattctttt tcgttcattt
    35761 aatcctcttt ttagaaaata tgtttgtaat ctaggacatc tgcaaagcgc tcctcccaaa
    35821 tgtcctcata aaattcatta atcgtttctg ctggaatgtc atccccatca aaggttgtga
    35881 cagcaccttc tggaacaata agctgatagc catattcaaa ggcaatcttg acagaggtat
    35941 ccacacaata ttctgtctgc ataccacata aaactaattt ttcaatcccc tgcttatcca
    36001 agtattcttt taagccagtt tctttgaaaa tactgttata tttcttctga aagacctttt
    36061 cagcaggttt tcgatttaaa agcgcagata actgccaatc ttctgatgtt tgagcttcag
    36121 agttctcaat atgttgaaca tagataattt caatattctt gcttctagcc tggttttgta
    36181 aataagaaat tttttccaat agactttttg tctggaaccc agtttctact aaaacattct
    36241 gaacatcaat gattataaaa gccatcttca tttagttctc tttcaaatag tgcacaaagg
    36301 cgttataggt ttcaagggtt tggggataga tggggaatcc ctgatgagct agatactcca
    36361 cggtacgagc tgtttcgtag gccaccgcct tattgagcga tagacttgca acctcacgcg
    36421 ccacatctac tccaggaaag gatcgattgt gctcgatata gtctgctaca tagacaacct
    36481 tatctaggtc tgtcatctga ccagcaccga ctgtatggac ttcaatggct cgcaggattt
    36541 ctgaatcatg caaatccaaa tcttcctgaa ttttgtaaat tccgaccata ccatgccaga
    36601 cattattgcc ccagtttttg aggtcagggt ctagctggta acggtcaatc aagtctagaa
    36661 attcctgatc tgacaacttt ttagcatagt catgaagaag acctgctaga cttgctttct
    36721 caacatctac accaaagcgc tgagctaatt ccatagcggc acgctccaca cctagacaat
    36781 gggttagacg tttctcaggt agaagctctg ccattttttc caacaaggcc tcacgggagc
    36841 agttgatata gtcttgatag gacatcagta gagcccctcc ttctcgatgt agtctagcac
    36901 tggctgaggt aagagaaagt tgggtttccg accttgggca atgaagtccc gcaccatgct
    36961 ggacgagata tccatgagcg gtacgtccac ccagataact ggataggaag tccctacctt
    37021 gtagcgtgga cgctgaaccc ccacaaactg aaccatgtca accagttcat caattcggta
    37081 ccacttaggc agatagtcaa ccatgtcggc accgatgata aagtaataat ccgtatctgg
    37141 attcttctct gtcaaaatct tcatggtgtc gtaggtgtag gaaataccct tgcgctccaa
    37201 ctcaatggtt tcaatgacta ggccgtcaat tccctcaatt gccaactcaa gcatcttgag
    37261 acgatggtgt tcagggatgg tttccttttt atcaacgtga ggaggttggt attcaggcat
    37321 gagcagaact tgatccagtc ccaactgttg ccgtacttga tccgcaacaa tgagatgggc
    37381 attgtgaaca gggttaaaat tcccccctaa aatcccaact tgtttgcgtt ttttctcctt
    37441 gatttctggc tccaactcta ccttggtaaa gggagtcaat agttcgattg tcataggcta
    37501 gtccccttta tttctctata aaaacagtta tttggagttt cgatcttaga tttctttgac
    37561 tttcttagaa atcttgcgat tttctttctt gctagattgt ttaaacaaaa tcaagatgcg
    37621 tcctattttt tggactgtat ctacaccgat ttcttcttcc aaaatttcag ctacttcgtg
    37681 gatgttttca tctgtgtttt gtaagagagt aaccttgatt aattcacgcg catcaagagc
    37741 ttgacggacg ctggttttga tttggtcgtt gagtccattt ttcccgattt ggatgatagg
    37801 tttgagggtg tgtgcctggc tgttgaggaa ggcacgttgt tttgatgtta atgacataat
    37861 tttcttcctt gttttttgat agttatttta ggtggtgagg gacggtcgga actaattttc
    37921 caaagatttc atctttgaaa aatggatttc ctgacctcac aattgagcct tggtctcaat
    37981 tgtgcccctt gccaatctac agattggcaa tcacaccacg gcaataacta tcgctatatg
    38041 agctcacttt gttcgctcag cgattttgtt ttaaataatt gcttttcgtg taacgacggc
    38101 gacgccttct ggtgcccaga cggcgacttt tgctgtgcct gttacacgaa tccagcctag
    38161 gcctgagatg actaggtctg tcttatcttt aatggtaaag acatgctgga ctagccttgg
    38221 gaaatcttct ttttccttgc tatttggtgg tgtcagaaga gttcccaggt gcttatcgta
    38281 gaaagcacta gctccttcaa gcttgctacg atggagtttg agttcattat caaagaaagc
    38341 agtaaatcct tgcttttctc ctgctataaa gtcaaagcgt cccaaaccac ctaaaaatag
    38401 ggtttgctca ggattaagct gataggtctt aggcttgatt tcctttttag gactgacata
    38461 cttgaggttt ttggccgtca agtagtgagc catctggtgg cggtggataa ttcccggcgt
    38521 atcgtaaata taagatccgt cgtcaagcgg aatctctatt ttgtccaagg ttgtccctgg
    38581 gaagcgtgaa gtagtgatga cattctgatc acccgtgatt tcttggataa tagcattgat
    38641 tagagttgat tttccaacgt tggtcacacc gaccacatag acatcgcggc ccttacggta
    38701 gtgttcaatc ttgtcaatga cttccttaat ggcatattta ttttgtgctg aagttaggac
    38761 cacatcgact ggacgaagac cttcttcatg ggcacgtttc atgagccact ggctaatctt
    38821 accagactta actgacttag gaaggatatc ttttttattt cctaccaaga ggacatcatt
    38881 gcccgagacg aaacgtggta aacctgggat gacagatcca ttaaaatcaa agatatcaat
    38941 aacattgacc actaaagcat cactgtctcc cacctcgtgc aagagcttga ggaaatcatc
    39001 gttcgtcaac tggacatctg tgatttcatt gtagtggcgg agacggaaac agcgttggca
    39061 atagacttcg ccagtctcca aacctttttc aagtgccgac tggggggtaa aaccaagacc
    39121 agctttatct gtcgtctgaa tggttgctcc acaaccaata cagagaattt cttccatagt
    39181 taaattcctt ttttatatgt aatcggtccg tacttttcag tgatttttct cataacacga
    39241 cgctcacgag ttcggttaat ctgcgttttg attgagtcat gttggaccaa gggtttgact
    39301 aaaattgacc gaatccctgc acggtgggct gctcgtatat ctgtcatgag ctggtcacca
    39361 accatgacca cttccttttt gtcatagtgg aattccttca tagcacggtc aataccaaat
    39421 gtgaagggct tcaaggccca gtaaacgtaa tcaatcccaa atttctcaac tgctcgttga
    39481 acgcgttttt tggtgttatt tgacactacg ataatgccaa tacccgcgtc ccgaaggtca
    39541 tgtagccatt gcttcatctc tggcgttcca tcagggttgt tccaagcaat gagggtatta
    39601 tccaaatcga ccaaaacagc cttgattccc tgcgcctgca ggcttgggac tgtcagatca
    39661 tagactgctt ccacagcaaa atctggtata taattttcaa tcgccattct ggctcctttt
    39721 cttaactttt tttcgcaatt ttccttagta acaaggataa aacaatccac aaacctgcac
    39781 tagccaaact gatatagagc caagcatggg gctcatctgt taaaggtaag ggaacattca
    39841 ttccgaaaaa gcctgtcacg actgccaaaa cagctagcaa gactgaaatg atagtcaagg
    39901 ttgtcaaatt gtcattcaga ttattgttta gaatattgtt gtaagaggct gaaagctgct
    39961 gtaaaatctg agagattagg tctgtcatgg ataccagctg atgagcctca atcatggcat
    40021 catcaaactg ttctctctca atctcatcaa aactacgata caaggcatga ccttgaatat
    40081 gctctaacaa aatccgattt tgtttggcag ctgccgttag ataaaccata ccagtctcca
    40141 aatcagacag ggcaaagagg tttttcttgg tagttcgctg gcgcaagagg tcattgacct
    40201 catccctact cttgtccatc tgctcaatga caggatagta ggcattgctg ataatttcca
    40261 gactggcaaa gagaaactta taaatcgaaa gcgtgtcatg gctctccaga taacgagtca
    40321 tctgttcaat gacataggcg ttcttggtat tactaatggt aatcaggcga cgatgctcga
    40381 caataaaggt catgggaaaa gtctcatagt aggccttgtc cttttttacg tctaggacat
    40441 tatagataaa ggtaaccgtc ccactttcac ggtggtaatc catgtgggcg cgctcgtttc
    40501 tatccagtgc gtattcaatg gtttccttgt ccaaaccgta aatttcagaa aggtcttcta
    40561 gtttattcaa cttccctagg tctaggtcta tccaggtaca accattgccc aactgctttt
    40621 ctaaaaccat cttttctcct tattaaactc cctctattgt accacaaatt acttaaaata
    40681 acaggtctag tctgtgcgtg agaaattgct aacaactgtg atatttcggt tagggacaaa
    40741 gtgaagggct tgctcctcgc tacgaagctg tgtcgtacga attcccacac tgacaacctt
    40801 acccgataca gtaatcggtc catttgtcag aacgacctca tctcccacat ccagttgacg
    40861 ttcaaagagg atgaaaaagc cattgatgac atcagacaga aagccttggg ctcccatacc
    40921 aatcgctacc ccagcaatac cagctccagc cagcaaacta gaaactggca aacctaaaat
    40981 agacaaaatg cagtagagta aaaagaaata tagcgtataa ttaaacacat tttctagtaa
    41041 acgtgagatg gttttttggc gtccgacatc atgacgagac atttttagag aaggtttgac
    41101 aattctctgc accatggtat gaagcatttt tttagcaata taaaatacaa tcaaaagcag
    41161 taaaagagaa atgaccttgg ttagaatatt ctcgataatg gttgtcacat ctagcttttc
    41221 aatataagcc tgaataaatt tttgcatata aaactccttt atttcattat accatatttt
    41281 catcagtctc attctccata aatattcaaa aaatagacag aaaattc
//
LOCUS       DAVKZB010000006        39710 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKZB010000006 DAVKZB010000000
VERSION     DAVKZB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.006"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            9..89
                     /locus_tag="VXA80_000292"
     tRNA            9..89
                     /locus_tag="VXA80_000292"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VXA80_000293"
     tRNA            94..164
                     /locus_tag="VXA80_000293"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VXA80_000294"
     tRNA            179..251
                     /locus_tag="VXA80_000294"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            262..333
                     /locus_tag="VXA80_000295"
     tRNA            262..333
                     /locus_tag="VXA80_000295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Gln,seq:ttg)
     gene            343..426
                     /locus_tag="VXA80_000296"
     tRNA            343..426
                     /locus_tag="VXA80_000296"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Leu,seq:caa)
     gene            533..1810
                     /locus_tag="VXA80_000297"
     CDS             533..1810
                     /locus_tag="VXA80_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV0352848.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1886..3370
                     /locus_tag="VXA80_000298"
     CDS             1886..3370
                     /locus_tag="VXA80_000298"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV0352849.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVTGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQATTGDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            3431..4711
                     /locus_tag="VXA80_000299"
     CDS             3431..4711
                     /locus_tag="VXA80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0352850.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTETVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            4715..5335
                     /locus_tag="VXA80_000300"
     CDS             4715..5335
                     /locus_tag="VXA80_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0352851.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5501..6613
                     /locus_tag="VXA80_000301"
     CDS             5501..6613
                     /locus_tag="VXA80_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0352852.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEATTTSSEAKEVASSN
                     GATAAVSTYQSEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6936..7361
                     /locus_tag="VXA80_000302"
     CDS             6936..7361
                     /locus_tag="VXA80_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0352853.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7372..7740
                     /locus_tag="VXA80_000303"
     CDS             7372..7740
                     /locus_tag="VXA80_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV0352854.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            7847..8457
                     /gene="pcp"
                     /locus_tag="VXA80_000304"
                     /pseudo
     CDS             7847..8457
                     /gene="pcp"
                     /locus_tag="VXA80_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8518..9375)
                     /locus_tag="VXA80_000305"
     CDS             complement(8518..9375)
                     /locus_tag="VXA80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV0352855.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYQA
                     VLARKRPKA"
     gene            9505..10647
                     /gene="tgt"
                     /locus_tag="VXA80_000306"
     CDS             9505..10647
                     /gene="tgt"
                     /locus_tag="VXA80_000306"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV0352856.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10745..12562
                     /locus_tag="VXA80_000307"
     CDS             10745..12562
                     /locus_tag="VXA80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV0352857.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKNDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12715..13866
                     /gene="nagA"
                     /locus_tag="VXA80_000308"
     CDS             12715..13866
                     /gene="nagA"
                     /locus_tag="VXA80_000308"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV0352858.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVEPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14028..15086
                     /locus_tag="VXA80_000309"
     CDS             14028..15086
                     /locus_tag="VXA80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV0352859.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15237..15602
                     /locus_tag="VXA80_000310"
     CDS             15237..15602
                     /locus_tag="VXA80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV0352860.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15678..16619
                     /locus_tag="VXA80_000311"
     CDS             15678..16619
                     /locus_tag="VXA80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV0352861.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16567..17583
                     /locus_tag="VXA80_000312"
     CDS             16567..17583
                     /locus_tag="VXA80_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV0352862.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17585..17911
                     /gene="comGC"
                     /locus_tag="VXA80_000313"
     CDS             17585..17911
                     /gene="comGC"
                     /locus_tag="VXA80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV0352863.1"
                     /translation="MKKMMTFLKKAKVKAFTLIEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            17904..18308
                     /locus_tag="VXA80_000314"
     CDS             17904..18308
                     /locus_tag="VXA80_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV0352864.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18313..18573
                     /locus_tag="VXA80_000315"
     CDS             18313..18573
                     /locus_tag="VXA80_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352865.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18536..18997
                     /locus_tag="VXA80_000316"
     CDS             18536..18997
                     /locus_tag="VXA80_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0352866.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKEIAMGKSKADDFR
                     KTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18975..19423
                     /locus_tag="VXA80_000317"
                     /pseudo
     CDS             18975..19423
                     /locus_tag="VXA80_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            19526..20113
                     /locus_tag="VXA80_000318"
     CDS             19526..20113
                     /locus_tag="VXA80_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0352867.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVNSQILYSAEDLFQGNHSEFFLTVSQKSLA"
     gene            20174..21127
                     /locus_tag="VXA80_000319"
     CDS             20174..21127
                     /locus_tag="VXA80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0352868.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21178..22368
                     /locus_tag="VXA80_000320"
     CDS             21178..22368
                     /locus_tag="VXA80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV0352869.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22516..22887
                     /gene="rnpA"
                     /locus_tag="VXA80_000321"
     CDS             22516..22887
                     /gene="rnpA"
                     /locus_tag="VXA80_000321"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV0352870.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22862..23686
                     /locus_tag="VXA80_000322"
     CDS             22862..23686
                     /locus_tag="VXA80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0352871.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23705..24691
                     /locus_tag="VXA80_000323"
     CDS             23705..24691
                     /locus_tag="VXA80_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV0352872.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24742..25365
                     /locus_tag="VXA80_000324"
     CDS             24742..25365
                     /locus_tag="VXA80_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV0352873.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25496..26953
                     /locus_tag="VXA80_000325"
     CDS             25496..26953
                     /locus_tag="VXA80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0352874.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26976..27257
                     /locus_tag="VXA80_000326"
     CDS             26976..27257
                     /locus_tag="VXA80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0352875.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27334..27819
                     /locus_tag="VXA80_000327"
     CDS             27334..27819
                     /locus_tag="VXA80_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0352876.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27836..28501
                     /locus_tag="VXA80_000328"
     CDS             27836..28501
                     /locus_tag="VXA80_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV0352877.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLVDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28505..29368
                     /locus_tag="VXA80_000329"
     CDS             28505..29368
                     /locus_tag="VXA80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV0352878.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            29370..30074
                     /locus_tag="VXA80_000330"
     CDS             29370..30074
                     /locus_tag="VXA80_000330"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV0352879.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVTYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30253..31926
                     /locus_tag="VXA80_000331"
     CDS             30253..31926
                     /locus_tag="VXA80_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0352880.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32038..33129
                     /gene="ulaG"
                     /locus_tag="VXA80_000332"
     CDS             32038..33129
                     /gene="ulaG"
                     /locus_tag="VXA80_000332"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV0352881.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33243..35219
                     /gene="tkt"
                     /locus_tag="VXA80_000333"
     CDS             33243..35219
                     /gene="tkt"
                     /locus_tag="VXA80_000333"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0352882.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMNEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35336..35635
                     /gene="yajC"
                     /locus_tag="VXA80_000334"
     CDS             35336..35635
                     /gene="yajC"
                     /locus_tag="VXA80_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0352883.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELTAIKTVLPLKETASLEGAIEK"
     gene            35682..36110
                     /locus_tag="VXA80_000335"
     CDS             35682..36110
                     /locus_tag="VXA80_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV0352884.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36112..36522
                     /locus_tag="VXA80_000336"
     CDS             36112..36522
                     /locus_tag="VXA80_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352885.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGSFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36823..39474
                     /gene="adhE"
                     /locus_tag="VXA80_000337"
                     /gene_synonym="adhC"
     CDS             36823..39474
                     /gene="adhE"
                     /locus_tag="VXA80_000337"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV0352886.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12066 a         7100 c         8935 g        11609 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttggggtata gccaagcggt aaggcaaggg actttgactc
      301 cctcatgcgt tggttcgaat ccagctaccc cagttactat ttgccggcgt ggcggaattg
      361 gcagacgcgc tggactcaaa atccagtgtc cgcaaggacg tgccggttcg accccggccg
      421 ccggtatagt atagtgttag gaacgttgtt attcttcgtt ccttttttat attatttttg
      481 gtataattat agttattcaa attttattta gattaagaaa gtgtagggga gtatgtcttg
      541 ttctatcgat ttattaaaac atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt
      601 tgtcggaatt gagttggagt atcctgttgc aagtttagaa ggggatgcta cagatgttga
      661 agttataaag gatctatttc attatttagt ttctactttg gatctcaccg tagcaaaggt
      721 agatgatttt ggcaatctga tccagttagt agatccgata agtcaggatg ctattttatt
      781 tgaagtttcc tatacaacga ttgagtttgc atttggtaag gctgaaacga ttcaagaggt
      841 cgaaaatcgt ttcaataatt atatgaatgt aattcagaga aagttagctg aatcaaatca
      901 tgctattgtt ggctgtggta tccatcccaa ctgggataaa aatgagaatt gtccagtggc
      961 ttatccacgc tatcagatgt tgatggatta tttgaatttg agtagaaata ttattaaatc
     1021 agatttacat catttccctg aatatggtac ttttatctgt gggagccagg ttcagctgga
     1081 tatttcaaaa accaactact tgcgggtgat taatgctttt actcaaattg aagcggctaa
     1141 ggcttattta tttgcaaact ctgagttttc gggtgcggat tgggatacga aaatttcaag
     1201 ggatattttc tgggaagaat ctatgcatgg tatctatcca gagaatgttg gggtcaatgc
     1261 tagactcctt aatgatgaag ctgatttttt tgactatcta aatcattctg cgatttttac
     1321 tgcggaacgt gatgggcaga cctattattt ttatcctatt caggctgggg actatttggc
     1381 tacgcctgaa atccaagcat ttgctctgaa tggggatgag gttattattt acccccaaga
     1441 gaaggatttt gaaactcatc gtagttacca gtaccaagac ttaacgactc gaggaacagt
     1501 tgagtttcgt agtgtgtgta cacagccact tgataggact tttgcttctg cagcttttca
     1561 cttgggatta ttggttaatt tagacaagtt agaagcttac ttagaaacag cacctttctt
     1621 taaagtattt ggttatgatt acaagttttt aaggagacaa ttttctaaga aaaatcttac
     1681 agatgaggaa gaaactatga ttattgaatt ttccaaagac ttactcctac tagctgagga
     1741 gggactagtg gtgagaaata aggaagaaat gacctattta cagcctttga gagaagaatt
     1801 gagcctataa tttctcttat aaagggagaa ttttctgaaa aatcatgata taatggacga
     1861 gactatagat aaaggataga gagtaatgac attagtttat caatcaacgc gtgatgccaa
     1921 caatacagta actgccagcc aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt
     1981 tacaccggtt acttatccaa aggtagattt gaactttaac aaattgaaag atgcttctta
     2041 ccaggaagtt gctaagctag ttttgtcagc atttttagat gactttacag ctgaggagtt
     2101 ggactactgt atcaacaatg cctacgatag caaatttgat actccagcta ttgcaccatt
     2161 agtgaaatta gatgggcaat acaatttgga acttttccat ggttcaacga ttgcctttaa
     2221 ggatatggcc ttgtctattt tgccatactt tatgacgact gctgctaaga aacatggttt
     2281 ggagaacaag attgttatct tgacagcgac atctggtgac acggggaaag ctgctatggc
     2341 ggggtttgcg gatgtgcctg gtactgagat tatcgtcttt tatccaaagg atggtgtcag
     2401 caagattcaa gagttacaaa tgaccactca aactggcgac aatactcatg ttattgctat
     2461 tgatggtaac tttgacgatg cgcaaacaaa tgtgaagcac atgtttaacg acgtggctct
     2521 tcgtgaaaaa ttgactacca acaagttgca attttcatca gctaactcta tgaacattgg
     2581 tcgtctggtg ccacaaattg tttattatgt ttatgcttac gctcaattgg tcaagactgg
     2641 tgagatcgtg actggtgata agattaactt cacagtacca acaggaaact ttggaaatat
     2701 cttggctgcc ttttatgcca aacaaatcgg tctcccagtt ggtaaattga tctgtgcttc
     2761 aaatgacaac aatgttttga cagacttctt caagacacgt gtctatgaca agaaacgtga
     2821 gtttaaggta acaaccagcc catctatgga tatcttggta tcttcaaact tggagcgttt
     2881 gattttccat cttttgggga atgatgcggt taagacggct gaactcatga atgctttgaa
     2941 tagccaaggt caatatgagt tgactaattt tgatgcagag attttggacc tctttgcagc
     3001 tgaatatgcg actgaggaag aaactgcggc agaaatcaag cgtgtttatg agtcagattc
     3061 ttatatcgag gatcctcata cagcggttgc ctcagcagtg tatagaaaat accaggcgac
     3121 tactggagat gcgactaaga cagtgattgc ttcaacagct agtccctaca agttcccagt
     3181 agttgcagta gaagcgctaa cagggaaagt tggcttgact gattttgaag ctttggctca
     3241 attacatgaa atctcaggcg ttgcagtgcc accagcagtt gatgggcttg agacagctcc
     3301 tgttcgtcac aagacaatag tggcagctgc tgacatgcaa gtagcggttg aggcttattt
     3361 aggactttaa gatagaggga gtaaactcgg ttgggaaacc aactgagttt cttttcatca
     3421 ggaggagaga ttgtttaaga aaaataaaga cattcttaat attgcattgc cagctatggg
     3481 tgaaaacttt ttgcagatgc taatgggaat ggtggacagt tatttggttg ctcatttagg
     3541 attgatagct atttcagggg tttcagtagc tggtaatatt atcaccattt atcaggcgat
     3601 tttcatcgct ctgggagctg ctatttccag tgttatttca aaaagcatag ggcagaaaga
     3661 ccagtcgaag ttggcctatc atgtgactga ggcgttgaag attaccttac tattaagttt
     3721 ccttttagga tttttgtcca tcttcgctgg gaaagagatg ataggacttt tggggacgga
     3781 gagggatgta gctgagagtg gtggactgta tctatctctg gtaggcggat cgattgttct
     3841 cttaggttta atgactagtc taggagcctt gattcgtgca acgcataatc cacgtctgcc
     3901 tctctatgtt agttttttat ccaatgcctt gaatattctt ttttcaagtc tagctatttt
     3961 tgttctggat atggggatag ctggtgttgc ttgggggaca attgtgtctc gtttggttgg
     4021 tcttgtgatt ttgtggtcac aattaaaact gccttatggg aagccaactt ttggtttaga
     4081 taaggaactg ttgaccttgg ctttaccagc agctggagag cgacttatga tgagggctgg
     4141 agatgtagtg atcattgcct tggtcgtttc ttttgggacg gagacagttg ctgggaatgc
     4201 aatcggagaa gtcttgaccc agtttaacta tatgcctgcc tttggcgtcg ctacggcaac
     4261 ggtcatgctg ttggcccgag cagttggaga ggatgattgg aaaagagttg ctagtttgag
     4321 taaacaaacc ttttggcttt ctctgttcct catgttgccc ctgtccttta gtatatatgt
     4381 cttgggtgta ccattaactc atctctatac gactgattct ctagcggtgg aggctagtgt
     4441 tctagtgaca ctgttttcac tacttgggac ccctatgacg acaggaacag tcatctatac
     4501 ggcagtctgg cagggattag gaaatgcacg cctccctttt tatgcgacaa gtataggcat
     4561 gtggtgtatc cgcattggga caggatatct gatggggatt gtgcttggtt ggggcttgcc
     4621 tggtatttgg gcagggtctc tcttggataa tggttttcgc tggttatttc tacgctatcg
     4681 ttaccagcgc tatatgaact tgaaaggata ggaaatgcaa aaaacagctt ttatttggga
     4741 tttagacggg actttattgg actcttacga agcgatttta tcagggattg aggagacttt
     4801 tgctcagttt tctattcctt atgataagga gaaggtgaga gagtttatct tcaagtattc
     4861 ggtgcaagat ttgcttgtgc gggtggcaga agatagaaat ctggatgttg aggtgctaaa
     4921 tcaggtgcgt gcccagagtc tggctgagaa gaatgctcag gtagttttga tgccaggtgc
     4981 gcgtgaggtg ctagcttggg cagacgaatc aggaattcag cagtttatat atactcataa
     5041 ggggaacaac gcttttacca ttctcaagga cttgggggtg gaatcctatt ttacagagat
     5101 tttaaccagt cagagtggct ttgtgcggaa gccaagtcca gaagcggcta cctatctgct
     5161 agataagtat cagttgaatt ctgataatac ttattatata ggggatcgga ctctggatgt
     5221 ggaatttgcc cagaatagtg ggattcaaag catcaacttt ttagagtcta cttatgaagg
     5281 gaatcacagg attcaagcgt tagcagatat ttcccgtatt tttgagacta agtgataaaa
     5341 agattgtgtc agttttgtga cagagaccta acaaactatt tcaagtaacc tagtttgtta
     5401 caaggaatag acagttctgt taaataggcc cgagagggct ttttttctac attttttgtg
     5461 ttatgataga caggtactca tttgaaagga atttgaaaga atgaagaaaa gaatgttatt
     5521 agcgtcaaca gtagccttgt catttgcccc agtattggca actcaagcag aagaagttct
     5581 ttggactgca cgtagtgttg agcaaatcca aaacgatttg actaaaacgg acaacaaaac
     5641 aagttatacc gtacagtatg gtgatacttt gagcaccatt gcagaagcct tgggtgtaga
     5701 tgtcacagtg cttgcgaatc tgaacaaaat cactaatatg gacttgattt tcccagaaac
     5761 tgttttgaca acgactgtca atgaagcaga agaagtaaca gaagttgaaa tccaaacacc
     5821 tcaagcagac tctagtgaag aagtgacaac tgcgacagca gatttgacca ctaatcaagt
     5881 gaccgttgat gatcaaactg ttcaggttgc agacctttct caaccaattg cagaagttac
     5941 aaagacagtg attgcttctg aagaagtggc accatctacg ggcacttctg tcccagagga
     6001 gcaaacgacc gaaacaactc gcccagttga agaagcaact cctcaggaaa cgactccagc
     6061 tgagaagcag gaaacacaag caagccctca agctgcatca gcagtggaag caactacaac
     6121 aagttcagaa gcaaaagaag tagcatcatc aaatggagct acagcagcag tttctactta
     6181 tcaatcagaa gaaacgaaag taatttcaac aacttacgag gctccagctg cgcccgatta
     6241 tgctggactt gcagtagcaa aatctgaaaa tgcaggtctt caaccacaaa cagctgcctt
     6301 taaagaagaa attgctaact tgtttggcat tacatccttt agtggttatc gtccaggaga
     6361 cagtggagat cacggaaaag gtttggctat cgactttatg gtaccagaac gttcagaatt
     6421 aggggataag attgcggaat atgctattca aaatatggcc agccgtggca ttagttacat
     6481 catctggaaa caacgtttct atgctccatt cgatagcaaa tatgggccag ctaacacttg
     6541 gaacccaatg ccagaccgtg gtagtgtgac agaaaatcac tatgatcacg ttcacgtttc
     6601 aatgaatgga taaacccgac ttgataacat cattttgacg aatgagatct agctttcgtg
     6661 atggaaagcg attctcgttc gttttttctt tgtcatactc ttcgaaaatc tcttcaaatc
     6721 acgtcagttt tatctgaaac ctcaaagctg tgctttgagc aacctgctac tagcttccta
     6781 gtttgctttt tgattttcat tgagtatcaa tttgaatgga aaatggaaag ttatcatctt
     6841 gtaatgagtt aagcaacatt cttgcaatct attttacttt atatcacaat taattagtca
     6901 aatattgata aatcaataaa aagagagggg aagaaatgct agagattcaa gatttactgt
     6961 atcaactccg cttgtctgag caagcgagta cgcaattgtt tgaaaaaagg cttgggatta
     7021 gtttgacacg gtatcagatt ttactgtttt tgctggagca ttctccttgt aaccaaatgg
     7081 cggttcagga gcgtttgaaa attgatcagg ctgctttgac acggcatttc aagattttgg
     7141 aaacggaagg tttggtggag cgtcatcgta atcctgaaaa tcagcgggaa gtgttggtag
     7201 aggctgcgaa gtatgccaag gagcagttag tggtgaatcc ccctctgcaa catatcaagg
     7261 ttaaggaaga gatggaaagt atcttaacag agtttgagag aacagaactc agccgtttat
     7321 taaataaatt ggttttgggt attgaaaata tagaaattta agtagaaata gatgtcaatt
     7381 attacaatta ttttaacaac gatcgttgct ttggagcatt tttacatttt ttatttggaa
     7441 agtattgcca cgcaatcaga tgcgactagt cgtgtattta atatggaaaa ggaagaattg
     7501 gctcatccgt cagtaagttc attgttcaaa aatcaaggaa tttataaggc tctgctagga
     7561 gtctttctct tgtatggcat ttatttctca cagaatttag aaattgtgac tatttttgtc
     7621 ttatttgtga ttggtgctgc gacttacggc tctttaacag cggataaaaa aattattttg
     7681 aaacaaggtg gatcagctat tttggccttg attagtattt tactctttaa atacacttga
     7741 aggtcgattc taatctcgct aatccttttt aatccagaat aagggaaata tgttatactt
     7801 gtttttaaga aaaaagtctc attgaattgg ttttgaggag ttagaaatga aagtattagt
     7861 gacaggtttt gagccctttg gaggggaaaa gggcaatcca gctttggagg ccattaaagg
     7921 tttaccagct gaaatccatg gtgctgaggt ccgttggcta gaggtgccga cagtttttca
     7981 caaatctgct caagtattgg aagaagagat gaatcgttat caacctgact ttgtcctttg
     8041 tattgggcaa gctggtggaa gaactagttt gacacctgaa tgagtggcca ttaatcaaga
     8101 cgatgcacgc atttctgata acgaagataa tcaaccgatt gaccgtccca ttcgcccaga
     8161 tggtgcttcg gcctacttta gtagtttgcc gattaaagcg atggttcaag ctataaaaaa
     8221 gaaggattac cggcctctgt ttccaatacg gcagggactt ttgtctgcag ccatttgatg
     8281 tatcaggctc tctatttggt agaaaagaaa tttccatatg ttaaggcagg ttttatgcat
     8341 attccttata tgatggaaca ggtggtgaac agaccgacta ctccaactat gagtttagtg
     8401 gatattcggc gagggataga agcagcaatc ggcgctatga tagaacatgg agattaggaa
     8461 ctcaagttgg taggcggaga aactcattga tagaaaaaag cttgagggga aaaaccttca
     8521 agcttttgga cgttttcgag ccaatactgc ttggtaaaac ataattttag tgcattggat
     8581 ataaggtagg agtgaaaaac tagcaatgcc aaaggtaatc caattgagga agtaccaagg
     8641 aagaagctgt aaatctagga caaagtgctg gaacttgtag cccttcataa aggaacggct
     8701 agtttttagg attcgtcttg gtgggacctg tcctaggtct agactataac agagaagaaa
     8761 ttccacctgt gaataggcat aatactgtgg aatatagagg atattcccta caatgatcaa
     8821 gatgagactt gcaagaaagt agagtccaaa gaccatgagg aaacgctcgg tttcaactga
     8881 tgagagatct agatttggaa actcaggatg tagggtgacg aattttttgg ctaaaaagct
     8941 actataaaag aggaggtaaa tcccaagtaa attagggata ctccataaaa agagatagaa
     9001 acgtttgaga agtagggtca aaaaggtttg agaaaagcgc tcctcatcaa agagagctag
     9061 gctgtttttt acagatggct ccgttttaga atctttcatg agtgtcagtg ttgcatagac
     9121 ggaactggtc aaaagaatag tcccgataaa ggagactagt agaggaaaga ggtatgcttg
     9181 aagtatttgg ccaagtatgc tgaaaaatgg ctgttctaaa acagtcccgt ggatccgaga
     9241 taagggatta agaaaactag ataagatgac cagcatactg ggaaggatat agaggagaaa
     9301 gagacggggg gtgtcagcct gaaaatgttt tgactcctga cgaattgttt ttaaatcaat
     9361 ttttggatag ttcattctct tattatacca tagttcttat acatagttcg tgacagttcc
     9421 tacttttttt gataaaatca tacagtgtgt ccttgggcac actgtatgaa ctgggactgt
     9481 ctttcccagc ttcggaggta aaaaatgtca gattcaccaa tcaaatatcg tttgattaag
     9541 aaagaaaaac acacaggagc tcgtctagga gaaatcatca ctccccacgg tacctttccg
     9601 acacctatgt ttatgccagt tgggacacaa gccactgtca aaactcagtc acctgaagaa
     9661 ttgaaggaga tgggttcggg aattatccta tcaaacacct atcatctctg gcttcgccct
     9721 ggagatgaac tcattgcacg cgctggtggt ctccacaagt tcatgaattg ggaccagcct
     9781 atcttgacag atagtggtgg ttttcaggtt tattctttag cagatagccg taatatcaca
     9841 gaagaaggag taacctttaa aaatcatcta aatggttcta agatgttcct atccccagaa
     9901 aaagccatct ctattcagaa taatctgggt tcagacatca tgatgtcctt tgatgaatgt
     9961 cctcagtttt atcaacctta tgactacgtt aagaaatcga tcgagcgtac cagccgttgg
    10021 gctgagcgtg gtttgaaggc tcaccgtcgt ccacatgacc aaggtttgtt tggaattgtg
    10081 caaggtgcag gatttgaaga ccttcgccgc caatcagctc atgatcttgt cagcatggat
    10141 ttctcaggct actctatcgg tggtttggca gtgggagaaa cgcatgaaga gatgaatgcg
    10201 gtcttggact ttacaactca actgctacct gaaaataaac ctcgttatct gatgggtgtg
    10261 ggagcgccag atagcttgat cgatggggtc attcgtgggg tggatatgtt tgactgtgtc
    10321 ttaccgactc gaattgctcg taacgggact tgtatgacca gtcaaggacg tttggttgtg
    10381 aaaaatgccc agtttgctga ggactttacg ccactggatc ctgagtgtga ctgctacaca
    10441 tgtaataact atacacgcgc ttaccttcgt cacctgctca aggctgatga aacctttggt
    10501 atccgcttga ctagctacca caatctttac ttcttgctta acctgatgaa gcaagtgcga
    10561 caagccatca tggatgacaa tctcttggaa ttccgtgagt attttgtgga aaaatatggc
    10621 tataataagt caggacgtaa tttctaaaat ggaattgata taaaaaaatc ctaagttttc
    10681 tcttaggatt tttcttcttt ttttgataga ataaagtgta caatgaaagg aagaataaac
    10741 tcgtatgcgc attaaatggt tttccttgat taggattaca ggtttacttt tggtactctt
    10801 gtatcacttc tttcagacga tctttcctgg aggatttttc ggggtagatg tctttttcac
    10861 attttcaggt ttcctgatta cagctctact cattgaagaa ttttctaaaa accatgagat
    10921 tgatttgata ggatttttta gaagacgctt ttatcggatt gtgccacctg tggttttgat
    10981 ggtcttggtg accatgcctt ttactttctt ggttcgccaa gactatgttg ctggaattgg
    11041 tggccagatt gcgggcgtct taggctttat gaccaacttc tatgaactcc taacaggtgg
    11101 gagttatgaa tctcagttca ttcctcattt gtttgttcat aattggagtt tggcagttga
    11161 ggttcattac tatattcttt ggggattggc agtttggttc ttatccaaac aagctaaatc
    11221 aaatggtcag ttgaagggga tggtctttct cttatctgct gttgccttct tgatcagttt
    11281 cttctccatg tttattggta gttttctagt gacctcttat tcctctgttt atttctccag
    11341 tttaactcat gtctatccat tctttttggg aagtatgtta gcaactattg taggcgttcg
    11401 tcagacgact tccctcgtca agcagttgga taaaatctgg gatttacgaa agactttggt
    11461 agtttttgga ggaggctttg gtttcctagt tcttttgact ttctttgtca aattcactta
    11521 tctttttgcc tatcttatcg gcttcttact tgccagtctt gcagctcttg ccatgattct
    11581 ggcggcgcgt gtcttacatg aaaagacaca tcatatacag gagccgaaga ttatcagctt
    11641 tttagcggat actagctatg cggtttatct tttccattgg cctttctata tcattttctc
    11701 acagttgaca tcaaatcttc ttgctgtatt actgactctg atttgttctt atggctttgc
    11761 cagtctgtca ttctatgtat tggaaccttg gattgcaggc aagaacacac ctattgtcca
    11821 aacccttcgt cccctgcctt atattcacgc aattcttgca gcaggtacag gaatcttgac
    11881 catcattgtc tgcacggtga ccttgttggc accacaagtg ggagcgtttg agacagactt
    11941 gactgtcaat ggcttgaagc aagctgcaac aaatattggc cagaccaagg tgatggcaga
    12001 acgggcagat gcaaacagtt tgggaattgc tgatggcact atgttaattg gtgactcagt
    12061 ggctttaagg gcaaatacag cactacagac agctcttcct ggagcacaga ttaacgcgca
    12121 ggtcagcgta acaaccaaga ccgcaaatga aatcatgcta aataatagcc agaataaatt
    12181 tttacctaag acggtggtca ttgcgactgg ggtaaataat cctgagaatt acaagaatga
    12241 ctgggacagt atcgtgaaaa atcttcctaa gggacaccat atgattttgg tgactcctta
    12301 tgagggagat aagacaaaag agacctatgc catcgttgag aaggctgctg cctatatgag
    12361 agaattggca gagaagacac cttacattac gatagcagat tggaatcaag ttgcgaaaga
    12421 gcatccagaa atttgggctg gaacagacca ggttcatttc gggagtgaga gtagcactat
    12481 cgaagcagga gcaaaattgt atgcagatac gattgccaca gctttgcaga cagctcaaga
    12541 caagccggtt aaatcaaaat aacttgtatt aaaaaagaga agagttggag aaatccagct
    12601 cttttaaaat atctccaaaa aataggtcta taccatttac aaataaaaaa gaaaggttta
    12661 taatgtaatt gacataataa attgtagaat caatctttta aggaggttaa cattatgcct
    12721 aactatatta aagcggatca gtttttctac ccacacggag ttcgtcgagg tggttacttg
    12781 gaacttgtgg acggcaagtt tgggaaacat gtagagcaga ttcctgaagg ggctgaggtg
    12841 attgactata caggttatag cattgcccca ggtcttgtgg atactcatat tcatggatat
    12901 gcaggtgtag atgtgatgga caacaacatt gaaggtacat tgcatactat gagtgaagga
    12961 cttcttagta ccggtgttac cagtttctta cccacaactt taacagccac ttatgagcaa
    13021 ttgcttgcag tcactgaaaa tcttggaaac cattataaag aagcaacagg tgctaagatt
    13081 cgtgggattt attatgaagg tccatatttc acagaaactt ttaagggggc acaaaatcca
    13141 acttatatga gagacccggg tgttgaggag tttcattctt ggcaaaaagc ggcaaatggc
    13201 ttgcttaata aaattgccct tgcaccagaa cgtgatgggg tggaagactt tgttcgtaca
    13261 gttacgggcg aaggtgtgac ggttgctctt ggacattcaa acgcgacttt tgatgaagcc
    13321 aaaaaagcag tcgatgctgg agcgagtgtt tgggtgcatg cctacaatgg aatgcgtggg
    13381 ttgactcacc gtgaattggg tatggttgga gccatgtacc aattgccaca tacctatgca
    13441 gagttgatct gtgatggtca ccacgtagaa ccaaaggctt gcgaaattct tatcaaacaa
    13501 aaaggaacag aaaacatcgc tcttatcaca gactgtatga cagctggggg attggaagac
    13561 ggagattata tgttgggaga attcccagtt gtcgttgcaa atggaactgc acgcctcaaa
    13621 tcgacaggta acttggcagg ttctatcctc aaactcaaag atggtttgaa gaatgtggtc
    13681 gaatggggaa ttgcgaatcc gcatgaagca gtcatgatgg ccagcttcaa cccagctaaa
    13741 tccgttcaca tcgatgacgt ctgtggccaa atccgtgaag gctacgacgc tgacttcatc
    13801 gtattagata aagatttgga attggtagca acctacctag atggcgtaaa acgttatcaa
    13861 gcataagaga gaaagcagaa gttagagact aacttctgct tttttataca gggtacttta
    13921 ctggtaaata aaaagttaaa aaaatcacaa aaaagcttga agaaacaaag aaaaaggctt
    13981 acaattgaac tataaatatt acaaataaaa aaacggagga gtgctttatg aaagcctata
    14041 cttatgttaa accaggactt gcttcttttg ttgatgtaga caaaccagtt attcgcaagc
    14101 caacagacgc tattgtgcgt attgtaaaaa ccactatttg tggaacagac ctccatatta
    14161 tcaaagggga tgttcctact tgccaaagtg gtaccattct tggccacgaa gggattggga
    14221 ttgttgaaga agttggggaa ggagtttcca acttcaaaaa aggtgacaag gtcttgattt
    14281 cttgcgtctg tgcctgtggt aaatgctact actgtaaaaa aggaatttat gcccactgtg
    14341 aagacgaagg gggctggatt ttcggtcact tgattgatgg tatgcaggct gaatatctac
    14401 gtgtccctca tgcagataat actctttacc atactccaga agacttgtca gatgaagctt
    14461 tggttatgct gtcagacatt ctgcctactg gatatgaaat tggtgtctta aaagggaaag
    14521 tagaacctgg ttgcagcgta gccattattg gttcaggtcc agttggattg gctgctcttt
    14581 taacagccca attctattca ccagctaaat tgattatggt agacctagac gataaccgct
    14641 tggaaactgc cctatcattc ggtgcgactc ataaggttaa ttcttcagac cctgaaaaag
    14701 ccattaaaga aatttatgat ttgacagatg gtcgtggtgt ggatgtcgct atcgaagctg
    14761 ttggtattcc tgcaacattt gatttctgtc aaaagattat cggtgtagac ggaacggttg
    14821 ccaactgtgg tgtgcatggt aaaccagttg aattcgattt agataaactt tggattcgta
    14881 acatcaatgt aacaactggt ttggtatcta caaatacgac tccacaattg ttgaaagcac
    14941 ttgaaagtca taagattgaa ccagaaaaat tggtaactca ctatttcaaa ctcagtgaaa
    15001 ttgaaaaagc ctatgaagtc ttcagtaagg cagcagacca ccatgccatt aaggtcatta
    15061 tcgaaaacga tatctcagaa gcctaagtag taaaaatatt tttgtacata agtaaataga
    15121 aattcagtca tccatcagat ggctgaattt tttatcaaaa aattaagaaa tgagcatatt
    15181 tctttccttg tctggcggaa ttggttataa tatacggtac aaaggaatga atgaatatgt
    15241 atcgtgttat agaaatgtac ggagattttg aaccgtggtg gttcttagaa ggttgggaag
    15301 aagatattgt agcaagtaga aaatttgacc agtattatga tgctctcaaa tactacaaaa
    15361 cttgctggtt tagattggaa caagaatccc ctctttataa aagtagaagc gacttgatga
    15421 ccattttttg ggacccggaa gaccaacgct ggtgtgatga atgtgatgag tatttacaac
    15481 aataccattc tttggccctt ttgcaggatg agcaggttat cccagacgaa aaactacgct
    15541 caggctatga aaaacaaacc agtcaggaaa ggaatcgttc ttgccgtatg aaattaaaat
    15601 agagaaaagt aacttttttg gagttgcttt ttttattttt ctaactcttt gcgaatagta
    15661 taggtgagga ggtaagtatg gttcaagaaa ttgcacaaga aatcattcgt tcagctcgga
    15721 aaaaagggac gcaggatatt tattttgtcc ctaagttaga cgcctatgag cttcatatga
    15781 gggtaggaga cgagcgctgt aaaattggta gctatgattt tgaaaagttt gcagccgtta
    15841 tcagtcactt taagtttgtg gcgggtatga atgtgggaga aaaaagacgt agtcaactgg
    15901 gttcctgtga ttatgcctat gaccagaaga tggcgtctct acgtttatct actgtaggcg
    15961 attatcgggg gcatgagagt ttggttatcc gtttgttgca cgatgaggag caggacttgc
    16021 atttttggtt tcaggatatt gaagaattag gcaagcagta caggcaacgg ggactctatc
    16081 tttttgctgg cccggttggg agtggtaaga cgaccttgat gcatgaattg tccaagtcac
    16141 tctttaaagg acagcaagtt atgtccatcg aagatcctgt cgaaatcaag caggacgaca
    16201 tgcttcagtt gcagttgaac gaagcaatcg gcctaaccta tgaaaatcta atcaaacttt
    16261 ccttgcgtca tcgaccagat ctcttgatta tcggagaaat tcgtgacagc gagacggcgc
    16321 gtgcagtggt cagagctagt ttgacaggtg cgacagtctt ttcaaccatt cacgccaaga
    16381 gtatccgagg tgtttatgag cgtctgctgg agttgggtgt gagtgaagaa gaattggcag
    16441 ttgttctgca aggagtctgc taccagagat taatcggggg aggaggaatc gttgactttg
    16501 caaacagaga ttatcaagaa caccaagcag ccaagtggaa tgagcaaatt gaccagcttc
    16561 ttaaagatgg acatatcaca agtcttcagg ctgagacgga aaaaattagc tacagctaag
    16621 caaaaaaata tcatcaccct atttaacaat ctcttttcta gcggttttca tctggtggag
    16681 accatctcct ttttagatag gagttccttg ttggacaagc agtgtgtgac ccagatgcgt
    16741 acaggtttgt ctcaggggaa atcattctca gaaatgatgg aaagtttggg atgttcaagt
    16801 accattgtca ctcagttatc tctagctgaa gttcatggca atcttcacct gagtttggga
    16861 aagatagaag aatatctaga caatctggcc aaggtcaaga aaaagttaat tgaagtagcg
    16921 acctatcctt tgattttgct gggttttctt ctcttaatta tgctggggct acggaattac
    16981 ctgctcccac aactggatag tagcaatatt gccacccgaa ttatcggtaa tctgccacaa
    17041 atttttctag gcatggtagg gcttgtttcc gtgcttgccc ttttagcact aactttttat
    17101 aaaagaagtt ctaagatgag tgtcttttct atcttagcac gccttccctt tattggaatc
    17161 tttgtgcaga cctacttgac agcctattat gcacgtgaat gggggaatat gatttcacag
    17221 ggaatggagc tgacgcagat ttttcaaatg atgcaggaac aaggttccca gctctttaaa
    17281 gaaatcggtc aagatctggc tcaaaccctg aaaaatggcc gtgaattttc tcagacgata
    17341 ggaacctatc ctttctttag gaaggaattg agtctcatca tagagtatgg ggaagttaag
    17401 tccaagctgg gtagtgagtt ggaaatctat gctgaaaaaa cttgggaagc cttttttacc
    17461 cgagtcaacc gcaccatgaa tttggtgcag ccactggttt ttatctttgt ggcactgatt
    17521 atcgttttac tttatgcggc aatgctcatg cccatgtatc aaaatatgga ggtaaatttt
    17581 taaaatgaaa aaaatgatga cattcttgaa aaaagctaag gttaaagctt ttacattgat
    17641 tgagatgttg gtggtcttgc tgattatcag cgtgcttttc ttgctctttg tacctaatct
    17701 gaccaagcaa aaagaagcag tcaatgacaa aggaaaagca gctgttgtta aggtggtgga
    17761 aagccaggca gaactttata gcttagaaaa gaatgaagat gctagcctaa gaaagttaca
    17821 agcagatggg cgcatcacgg aagaacaggt taaagcttat aatgaatact atactaaaaa
    17881 tggaggggca aatcgtaaag tcaatgatta aggcctttac catgctggaa agtctcttgg
    17941 ttttgggact tgtgagtatc cttgccttgg gcttatccgg ctctgtccag tccacttttg
    18001 cagcagtaga ggagcagatt ttctttatgg agtttgaaga actctatcgg gaaacccaaa
    18061 aacgcagtgt agccagtcag caaaagacta gtctaaattt agatgggcag acgcttagca
    18121 atggcagtca aaagttgaca gttcctaaag gaattcaggc accatcaggc caaagtatta
    18181 catttgaccg agctgggggc aattcgtccc tggctaaggt tgaatttcag accagtaaag
    18241 gagcgattcg ctatcaatta tatctaggaa atggaaaaat taaacgcatt aaggaaacaa
    18301 aaaattaggg cagtgatttt actggaagca gtagtcgctc tagctatctt tgccagcatt
    18361 gcgaccctcc ttttgggaca aattcaaaaa aataggcaag aggaagcaaa aatcttgcaa
    18421 aaggaagaag tcttgagggt agctaagatg gccttgcaga caggtcaaaa tcaggtaagc
    18481 atcaacggag ttgagattca ggtattttct agtgaaaaag gattggaggt ctaccatggt
    18541 tcagaacagt tgttggcaat caaagagcca taaggtcaag gcttttacct tgttagaatc
    18601 cctgcttgcc ctgattgtca tcagtggggg attactcctt tttcaagcta tgagtcagct
    18661 cctcatttca gaagttcgct accagcaaca aagcgagcaa aaggagtggc tcttgtttgt
    18721 ggaccaactt gaggcagaat tagaccgttc gcagttcgaa aaagtagaag gcaatcgcct
    18781 atacatgaag caagatggca aggagattgc tatgggtaag tctaaggcag atgatttccg
    18841 taaaacgaat gctcgtggtc gaggttatca gcctatggtt tatggcctca aatcagctca
    18901 gattacagag gacaatcaac tggttcattt tcgtttccag tttcaaaaag gcttagaaag
    18961 ggagttcatc tatcgtgtgg aaaaagaaga aagttaaggc aggtgttctc ctctatgcag
    19021 tcaccatggc agctatcttt agtcttttgt tgcaatttta cttgaaccga caagtcgccc
    19081 actatcaaga ctatgctttg aataaagaaa aattggttgc ttttgctatg gctaaacgaa
    19141 ccaaggataa agccgagcaa gaaagtgggg aacaggtctt taatctaggt caggtaagct
    19201 atcaaaacaa gaaaacaagc ttggtgacga ctgttcgtac gtctaagagc caatatgagt
    19261 ttctgtttcc ttcagtcaaa atcaaagaag agaaaagaga taaaaaggaa gaggtagcga
    19321 ccgattcaag cgaaaaagtg gagaagaaaa aaatcagaag agaagcctga aaagaaagag
    19381 aattcctagt caattcaact ataatgcgtt gaatccagaa tagtccactg tagtttctag
    19441 aaaattgctg gaaatggatg ttaagctcca attcatttgt ttatatctta tttcagttta
    19501 ctatactttg tgctaaacta aagatatgaa acatgatttt aaccacaaag cagaaacttt
    19561 cgactcccct aaaaatatct tcctcgcaaa cttggtatgt caagcagtcg agaaacagat
    19621 tgatatttta tcagacaaag taattttaga tttcggtggt ggcacgggtc tattagcctt
    19681 gcccctagcc aagcaggcta agtcagttac tcttgtagac atttctgaga aaatgttgga
    19741 gcaagctcgt ttgaaagcgg agcagcaagc aatcaagaat atccagtttt tggagcaaga
    19801 tttaccgaaa aatcccttgg agaaagagtt tgattgcctt gctgttagtc gggttcttca
    19861 tcatatgcct gatttggatg cggctctctc actgtttcat caacatttga aggaagatgg
    19921 gaaactcatc attgctgatt ttaccaagac agaagctaat catcatggat ttgatttagc
    19981 tgaactggaa aacaagctaa ttgagcatgg tttttcatct gtgaatagtc agattctcta
    20041 tagcgctgaa gacctgtttc aagggaatca ctcagaattc tttttaacag tatcccaaaa
    20101 atcactcgcc tagtcaggga gtgatttttc tataaggatg gaaaaaagaa gggaaatttg
    20161 ataagatagg aatatggatt ttgaaaaaat tgaacaagct tatatctatt tactagagaa
    20221 tgtccaagtc atccaaagtg atttggcgac caacttttat gacgccttgg tggagcaaaa
    20281 tagcatctat ctggatggtg aaactgagct aaaccaggtc aaagacaaca atcaggccct
    20341 taagcgttta gcactacgca aagaagaatg gctcaagacc taccagtttc tcttgatgaa
    20401 ggctgggcaa acagaaccct tgcaggccaa tcaccagttt acaccggatg ctattgcttt
    20461 gcttttggtg tttattgtgg aagagttgtt taaagaggag gaaattacta tcctcgaaat
    20521 gggttctggg atgggaattc taggcgctat tttcttgacc tcgcttgata aaaaggtgga
    20581 ttacttggga atggaagtgg atgatttgct gattgatctg gcagctagca tggcagatgt
    20641 aattggtttg caggctggct ttgtccaagg agatgccgtt cgcccacaaa tgctcaaaga
    20701 aagcgatgtg gtcatcagtg acttgcctgt cggctattat cctgatgatg ccgttgcgtc
    20761 gcgccatcaa gttgcttcta gccaagaaca tacttacgcc catcacttgc tcatggaaca
    20821 agggcttaag tacctcaagt cagacggata cgctattttt ctagctccga gtgatttgtt
    20881 gaccagtcct caaagtgatt tgttaaaaga atggctgaaa gaagaggcga gtctggttgc
    20941 tatgattagt ctgcctgaaa atctctttgc taatgccaaa caatctaaga ctatttttat
    21001 cctacagaag aaaaatgaaa tagcagtaga gccttttgtt tatccacttg ctagcttgca
    21061 agatgcaagt gttttaatga aatttaaaga aaattttcaa aaatggactc aaggtactga
    21121 aatataaaat agattttgtt ataatagttg aaaacgctta aaaaggggta tcatgttatg
    21181 acaaaaacaa ttgcaatcaa tgcaggaagt tcaagtttga aatggcaatt atacttaatg
    21241 ccagaggaaa aagtgttagc caaaggtttg attgaacgta tcggtttgaa agattcaatt
    21301 tcaactgtaa aatttgacgg ccgttctgaa caacaaattt tggatatcga aaatcatata
    21361 caagccgtta aaattttatt ggatgacttg attcgtttcg atattatcaa ggcttatgac
    21421 gagattacag gtgttggaca tcgtgttgtt gctggtggag aatatttcaa agaatcaaca
    21481 gttgttgagg gagatgtttt agaaaaagtt gaagagttga gtttgttggc tcctctacac
    21541 aacccggcca atgcagcagg tgttcgtgcc ttcaaggaat tgttgccaga cattaccagt
    21601 gtagttgttt ttgatacttc cttccacaca agtatgccag agaaagctta tcgctaccct
    21661 ctaccaacaa aatattacac agaaaacaag gttcgtaaat acggtgctca tggtacaagt
    21721 caccagtttg tagcaggaga agctgcaaaa ctcttgggac gtccattaga agacttgaag
    21781 ttaattacct gtcatattgg taacggaggc tcaattacag ctgtgaaagc cggcaaatct
    21841 gtagacactt ctatggggtt cactcctctt ggtggtatta tgatgggaac gcgtacaggg
    21901 gatattgatc cagctatcat tccttattta atgcaatata cagaggattt taacacacca
    21961 gaagatatca gtcgtgttct taaccgtgaa tcaggtcttt tgggagtttc tgctaattct
    22021 agcgatatgc gcgatataga agcagctgta gcagaaggga atcacgaggc tagcttggct
    22081 tatgaaatgt atgttgaccg tatccaaaaa catatcggtc agtaccttgc agtgctaaat
    22141 ggagcagatg ccattgtttt cacagcaggt gtcggtgaaa atgcagagag tttccgtcgt
    22201 gatgtaatct cagggatttc gtggtttggt tgtgatgttg atgatgaaaa gaatgtcttt
    22261 ggcgttacag gagacatctc aacagaggca gctaaaatcc gtgtcttggt tattccaaca
    22321 gatgaagaat tagtcattgc ccgtgacgtt gaacgcttga aaaaataagt gaaactaaaa
    22381 aatattcaat acaaggagtt gggaaagtta tttttccagc ttctttttct gatgaaattg
    22441 tccaaaacct tgctatgatt ggcttttttg aaaaatatgg tataatagta gtaatttaat
    22501 agatggagtt tagttttgaa gaaaaacttt cgtgtaaaaa gagagaaaga ttttaaggcg
    22561 attttcaagg aggggacaag ttttgctaat cgcaaatttg tggtctacca attagaaaac
    22621 cagaaaaacc attttcgagt aggtctatca gttagcaaaa aactggggaa tgccgtcact
    22681 agaaatcaaa ttaagcgacg gattcggcat attatccaga atgcaaaagg gagtctggta
    22741 gaagatgtcg actttgttgt cattgctcga aaaggagtcg aaaccttggg atacgcagag
    22801 atggagaaaa atctactcca tgtattaaaa ttatcaaaga tttaccggga aggaaatggg
    22861 agtgaaaaag aaactaaagt tgactagttt gctaggactg tctctgttaa tcatgacagc
    22921 ctgtgcgact aatggggtaa ctagcgatat tacagccgaa tcggctgatt tttggagtaa
    22981 attggtttac ttctttgcgg aaatcattcg ctttttatcg tttgatatta gtatcggagt
    23041 ggggattatt ctctttacgg tcttgattcg tacagtcctc ttgccagtct ttcaggtgca
    23101 aatggtggct tctaggaaaa tgcaggaagc tcagccacgc attaaggcgc ttcgagaaca
    23161 atatccaggt cgagatatgg aaagcagaac caaactagag caggaaatgc gtaaagtatt
    23221 taaagaaatg ggtgtcagac agtcagactc tctttggccg attttgattc agatgccggt
    23281 tattttggcc ctgttccaag ccctatcaag agttgacttt ttaaagacag gtcatttctt
    23341 atggattaac cttggtagtg tggatacaac ccttgttctt ccgattttag cagcagtatt
    23401 caccttttta agtacttggt tgtccaacaa agctttgtct gagcgaaatg gcgctacgac
    23461 tgcgatgatg tatgggattc cagtcttgat ttttatcttt gcagtttatg cgccaggtgg
    23521 agtcgcccta tactggacag tgtctaatgc ttatcaagtc ttgcaaacct atttcttgaa
    23581 taatccattc aagattatcg cagagcgcga ggccgtagta caggcacaaa aagatttgga
    23641 aaatagaaaa agaaaagcca agaaaaaggc tcagaaaacg aaataaataa ggaggaatct
    23701 ggtaatggta gtatttacag gttcaactgt tgaagaagca atccagaaag gattgaaaga
    23761 attagatatt ccaagaatga aggctcatat caaagtcatt tctagggaga aaaaaggctt
    23821 tcttggtcta tttggtaaaa aaccagccca agtggatatt gaagcgatta gtgaaacgac
    23881 tgttgtcaaa gcaaatcaac aggtagtaaa aggcgttccg aaaaaaatca atgatttgaa
    23941 cgagcctgtg aagacggtta gtgaagaaac cgttgacctt ggtcatgtgg ttaatgctat
    24001 taaaaaaata gaggaagaag gtcaaggtat ttctgatgaa gtcaaggctg aaatcttaaa
    24061 acatgaaaga catgccagca ctatcttaga agaaactggt cacattgaga ttttaaatga
    24121 acttcaaatc gaggaagcga tgagggaaga agcaggcgct gatgaccttg aaactgagca
    24181 agatcaaact gaaaatcaag acttgaaaga gatgggcttg aaggtcgagc aaagttatga
    24241 tattgcccag gtggctacgg atgtgactgc ctatgttcaa gcgattgtgg atgacatgga
    24301 tgttgaggct acactttcaa atgattataa ccgtcgtagc atcaatctac aaattgacac
    24361 caacgaacca ggtcgtatta tcggctacca tggtaaagtc ttgaaggcct tgcaactgtt
    24421 ggctcaaaat tatctttaca accgctattc caaaaccttc tacgttacaa tcaatgtcaa
    24481 tgattatgtc gaacaccgtg cagaagtctt gcagacctat gcgcaaaaat tggcgaatcg
    24541 tgttttggaa gaaggtcgca gtcataaaac agatccaatg tcaaatagcg aacgcaagat
    24601 tatccatcgt attatttcac gtatggatgg cgtgactagt tactctgaag gtgatgagcc
    24661 aaatcgctat gttgttgtag atacagaata agtaaaatca gggttgacct gattttttgc
    24721 tagttagagg aggttaaact gatgttgaat aagataagag actatctaga cttttctggt
    24781 ttgcagtacc gtagtcctga taaagcggga gcagagcgag agaagatgct ggcgttccgc
    24841 cacaaaggac aagaggcccg aaaggctttt acagaactgg ccaaagcctt tcaagcaagc
    24901 catccagaat ggcaactcca acagactagt cagtggatga atcaggccca gcgtttgaga
    24961 ccacattttt gggtttatct acagagagac ggacaagtga cagaacctat gatggcctta
    25021 cgtttgtatg ggacatctac tgactttgga atttctttgg aagtcagttt catcgaacgt
    25081 aagaaggatg agcaaacact gggcaagcag accaaagttt tagacattcc aaccgttaaa
    25141 gggatttatt atctaaccta ctctaatggt caaagtcaac ggtgggaggc gaatgaagaa
    25201 aagcgtcgta ctttacgcga gaaggtgaga agtcaagaag ttcgaaaagt tttagtgaag
    25261 gtagatgttc ctatgacaga aaattcgtct gaagaagaaa tcgtagaagg cttattgaag
    25321 tcttattcta aaattcttcc ctattatcta gctacgagaa aataatataa tttgtaaaac
    25381 atcataaatc atacagtcca agagtgaaca gtccgctgtg taattcttgg tctttttgtt
    25441 tgcgctttcg cattatataa taaacttaca aaaacaattc aaaaggagaa caattatgga
    25501 agtcgtttca agtgttctaa attggttttc tagcaatatt ttgcagaatc ccgcattttt
    25561 cgtaggttta ttggtgttga taggatatgc acttttgaaa aaacctgccc atgacgtttt
    25621 ttcagggttt gttaaagcaa cagtagggta tatgttgctt aacgtgggtg ctggtggttt
    25681 ggttacaacc tttcgtccaa tcttagcagc tcttaactac aaattccaaa ttggtgcagc
    25741 ggttatcgac ccttactttg gacttgctgc agcaaacaac aaaattgcag cagagtttcc
    25801 agattttgtt ggaactgcaa ctacagctct attgattggt ttcggaataa atatcttgct
    25861 cgtagctctt cgaaagatta cgaaggtaag aaccctcttt attactggtc acatcatggt
    25921 acaacaagct gcaacagtat ctcttatggt tctattctta gtaccacaat tgcgcaatgc
    25981 ttacggtaca gcagcgattg gtatcatctg tggactttac tgggcagtta gttcaaatat
    26041 gactgttgag gcaactcaac gcttgactgg tggtggcgga tttgcgattg gtcaccaaca
    26101 gcaatttgca atctggtttg tagataaagt agcaggacgc tttggtaaga aagaagaaag
    26161 tttagacaat cttaaattac ctaagttcct ctcaatcttc cacgatacag ttgttgcatc
    26221 tgctaccttg atgctcgtat tcttcggagc cattctttta atcttgggtc cagacattat
    26281 gtctaataaa gaagtcatca cttcaggaac tctattcaat cctgctaaac aagatttctt
    26341 tatgtacatt atccaaacag cctttacctt ctcagtttac ttgttcgttt tgatgcaagg
    26401 tgtccgaatg ttcgtatctg agttgacaaa cgccttccaa ggtatttcaa acaaattgtt
    26461 gccaggttca ttcccagcgg ttgacgttgc agcttcttat ggatttggtt ctccaaatgc
    26521 tgtcttgtca ggatttacct ttggtttgat tggtcaattg attacaattg ttttgctcat
    26581 cgtctttaaa aatccgattc ttattattac aggatttgta ccagtgttct ttgacaatgc
    26641 agccattgcg gtctacgctg ataaacgcgg cggatggaaa gcggctgtta tcctttcctt
    26701 tatatcaggt gtccttcaag ttgctctagg agctctttgt gtggcccttc tcgatttggc
    26761 atcttatggt ggctaccatg gaaatatcga ctttgaattc ccatggcttg gatttggata
    26821 tatcttcaaa taccttggta ttgttggtta tgtacttgtg tgtctcttct tgcttgttat
    26881 tcctcaactt caatttgcca aagcaaaaga taaagagaaa tattacaacg gtgaagttca
    26941 agaagaagct tagtatctag aaaaggagaa ataaaatggt taaagtatta gcagcgtgcg
    27001 gaaatggaat gggttcatca atggttatca agatgaaggt tgaaaatgct ctccgtaagc
    27061 ttaatcaaac agattttaca gtcaattcat gcagtgtcgg tgaagctaaa ggtttagcag
    27121 taggatatga catcgtaatc gcttctcttc atttgattca agaattggaa gggcgaacta
    27181 atgggaagtt aattgggctt gataacttga tggatgataa agaaatcacc gaaaaactca
    27241 gtcaagcact acagtaaaag gttggagggg gctggacaga aactgagagt tatcgtttct
    27301 gtccttctcc ctctttaaat aaaggaggca gatatgaatt taaaacaagc tttaattgac
    27361 aatgactcga tccgactagg tttagaggct aacaattgga aagaagcagt caaggtagca
    27421 gtagatccct taattgaaag tggggcaatt ttgccagagt attacgatgc tatcattgaa
    27481 tcgactgaag agtatgggcc ttactatatc ttgatgccag gtatggctat gccccacgct
    27541 agacctgaag ctggtgtgca aagtgatgcc ttttcattga ttaccttaca aaatcctgtt
    27601 gtattttcag atgggaaaga ggtatctgtt ttgttggcac tagcagcaac aagttcaaaa
    27661 attcacacaa gtgtagccat tccacaaatt attgccctgt ttgaattaga agattctatt
    27721 gcacgtttac aggcttgcca gactaaagaa gatgtcttgg ctatgattga agaatctaag
    27781 gatagccctt atctcgaagg attggatttg gaaagttaga aagaggaata aagaaatgac
    27841 aaaaagaata cctaatttac aagttgcatt agaccattca gacttgcaag gagcgattaa
    27901 agcagctgtt tctgttggtc aggaagtaga tattatcgaa gctggaactg tttgcttgct
    27961 tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt ttcccagata agattattgt
    28021 ggcagacaca aaatgtgctg atgctggtgg aacagttgct aaaaataatg cggttcgtgg
    28081 agcagactgg atgacttgta tctgttgtgc aaccatccct actatggaag cagctctaaa
    28141 ggctatcaag actgaacgag gagaacgagg cgaaatccag atcgagcttt atggcgattg
    28201 gacttttgaa caagctcagc tttggctaga tgcaggtatc tcacaagcta tttatcacca
    28261 atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa aaagacctta ataaggttaa
    28321 aaaactcgtt gacatgggct tccgtgtatc tgtaacaggt ggtctagatg tagatactct
    28381 caaactcttt gaaggtgttg atatctttac ctttatcgca ggtcgtggaa ttacagaggc
    28441 tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa atcaaacgaa tttgggggta
    28501 aatcatggta cgtccaattg gaatttatga aaaggcaacc ccaacacact ttacttggct
    28561 agaacgttta aattttgcca aggagttagg ctttgatttt gtcgagatgt ctattgacga
    28621 acgtgacgag cgtttagcaa gacttgactg gagtaaggaa gaacgcttgg aagttgtcaa
    28681 agcaatctat gaaactggtg ttcgtattcc ttctatctgt ttttcaggcc atcgtcgcta
    28741 cccattgggt tcaaaagatc cagttctaga ggaaaaatct ctagaactca tgaaaaaatg
    28801 tatcgaatta gctcaagact tgggagttcg tacgattcaa ttagctggtt acgatgttta
    28861 ctatgaggaa aagtcacccc agacacgcca acgttttatc aaaaatttga gaaaagcctg
    28921 tgactgggct gaagaagctc aggtggtact tgctattgaa attatggatg atcctttcat
    28981 cagtagcatc gaaaaatatt tggctataga aaaagagatt gactctccct tcctctttgt
    29041 atatccagat attggtaatg tgtctgcatg gcataatgat atctatagtg agttttatct
    29101 tggtcatcat gccatcgcag ctctccatct caaggatact tatgcagtga cagaaagttc
    29161 aaagggccag ttccgagatg tacctttcgg gcaaggttgt gtcaaatggg aagaagcttt
    29221 cgatatttta aaggaaacca attataatgg acctttccta atcgaaatgt ggtctgaaaa
    29281 ttgtgaaaca gtagaagaaa cacgcgccgc cgttcaagag gcgcaagctt ttctctatcc
    29341 actcattaag aaagcaggtt tgatgtaaga tgaatcaagt aatcaatgct atgcgtaaac
    29401 gagtctgtga tgccaatcaa tcattgccaa aacatggact tgtcaaattt acctggggga
    29461 atgtatctga agttaatcgc gaactcggtg tcattgttat caaaccatca ggcgtggatt
    29521 atgacgaatt gacacctgaa aacatggtag tgactgatct agatggtaag atcctagaag
    29581 gggatttaag accatcttcc gacctcccaa ctcatgtgca attatataag gcttggtcag
    29641 aaattggtag tgtggttcac acccattcga cagaagctgt tggttgggct caggcaggtc
    29701 gtgatattcc tttctacgga acaacccatg cagattattt ctacggttca atcccttgcg
    29761 cccgtagttt gaccaaggac gaagtagaag tgacctatga aaaagatact ggcctggtta
    29821 tcgtagaaga gtttgaacat cgcggactta acccggttga agtaccagga attgttgtac
    29881 gcaatcacgg tccattcacc tggggcaaaa atccagagaa tgctgtttat cactctgtcg
    29941 tactagagga agtatcaaag atgaatcgct ttacagaaca aatcaatcca agagttggac
    30001 ctgctcccca gtacatacta gaaaaacact accaacgtaa acatggacca aatgcttatt
    30061 atggtcaaaa gtaagaacga tgaaggagga gaaaaagata aatttagctc ctctttttac
    30121 atttgatttt tattgagagt aaagttggag ttgaagtaat tttaaaagat tttttagaaa
    30181 tagcgcttga tatatatatg gtaaaataaa aagaattgct gtgatatcaa tagatttggg
    30241 ggatttttta atatggtact ggataaggca agttgtgatt tgcttcaata tttgatggat
    30301 caagaaacgt ccaaaacgat tatggcgatt tcgaaagatt tgaaagagtc aagaaggaaa
    30361 atttattatc acattgacaa aatcaatgct gctctgggtg acgaggcgct tcacatcatt
    30421 agtattccac gaattggtat tcacttaacg gaagagcaga gagatgcttg ttgtaaacta
    30481 ttatcggaag tagattcgta cgattatatc atgagtgcgc atgaacgtat gatgataatg
    30541 ttactatgga taggtatttc taaagaacgt attacgattg aaaaattgat agagttaaca
    30601 gaggtatcta ggaatactgt tctcaatgat ttgaatagta ttcgttatca actaactttg
    30661 gaacaatatc aggtgatctt gcaagtgagc aagtcacagg gataccacct tcatgcccac
    30721 cctcttaata aaattcagta tcttcaatcg cttctatatc atatttttat ggaagaaaat
    30781 gccacttttg tatctatttt agaagataag atgaaagaga ggttagatga tgagtgtttg
    30841 ctttctgttg aaatgaacca attttttaag gaacaggttc ctttagttga acaagattta
    30901 gggaagaaaa taaaccatca tgaaataact tttatgttgc aggttctacc ttatttgctg
    30961 ttaagctgtc ataatgttga acagtatcaa gaaagacatc aggatataga gcaagaattt
    31021 tctttgataa gaaaaagaat agagtatcag gtgtctaaga aattaggaga acggttgttt
    31081 caaaagtttg aaatttcttt gtcaggactt gaagtttctc ttgtagctgt tctcctcctc
    31141 tcctatcgta aagatttgga tattcatgca gaaagtgatg attttcggca attaaaactt
    31201 gctttagaag aatttatctg gtattttgaa tcacaaatcc gaatggagat tgagaacaag
    31261 gatgatttgt tacgaaattt gatgatccac tgtaaagcct tgttatttag aaagacttac
    31321 ggtatttttt ctaaaaatcc tctaacaaaa caaattcgat ccaagtatgg agaattattt
    31381 ttagtcacta gaaaatctgc ggaaatttta gaaggagcat ggtttattcg gctaacagac
    31441 gatgatattg cctatttgac gattcatatt ggaggatttt taaagtatac accatcgtct
    31501 caaaaaaata tgaaaaaagt ttatctcgtt tgtgatgaag gtgttgcggt ttcgagactt
    31561 ttgctgaaac aatgcaaact ttattttcca aatgagcaaa ttgacactgt atttacaaca
    31621 gaacaattta agagtgtgga agatattgca caagttgatg tagtgattac tactaatgat
    31681 gatttggata gcagatttcc gattttaagg gttaatccta tccttgaagc agaagatatt
    31741 ttgaaaatgc tagactatct taaacacaat atatttcgta atgagagcaa aagtttcagt
    31801 gaaaatcttt ctagtcttat ttcgtcttat attgtagaca gcaagttggc tagtaagttc
    31861 caagaagagg ttcaaacact tataaatcaa gaaatagtag ttcaagcttt tttggaagat
    31921 atttgaagga cagtccaatg atgaacacaa acctgtgttt ttcttggtct tttttagtgt
    31981 tttgaagggt ggtatactaa tctcaaagat aacaattata tccaaaggag gcaacatatg
    32041 ccaaacgtca aagaaattac aagagagtca tggattttag ccactttccc agagtgggga
    32101 acatggttga acgaagaaat cgaagaagaa gtcgtacctg aaggcaactt tgccatgtgg
    32161 tggctaggca actgtggtac ttggattaag acaccagctg gtgctaacgt tgtcatggac
    32221 ctttggtcaa accgtggaaa atcaaccaaa aaagtgaaag atatggttcg tgggcaccaa
    32281 atggcaaata tggcaggtgt tcgtaagctg caaccaaact tgcgtgttca gccaatggtt
    32341 atcgatccat ttgctatcaa cgaactagac tattacttag tttcacactt ccacagtgat
    32401 catatcgacc catacacagc tgcagcaatt ctcaacaatc ctaagttaga gcatgttaag
    32461 tttatcggtc cttaccactg tggacgaatc tgggaaggat ggggtgttcc aaaagaacgt
    32521 atcatcgttg ttaaaccagg tgacactatc gaattaaaag atatgaagat tcatgcagta
    32581 gaatcatttg accgtacttg cttggtaact ctcccagtga acggtgctga tgagacaggc
    32641 ggtgaacttg ctggcttggc tgttacagat gaagaaatgg ctcaaaaggc tgttaactat
    32701 atctttgaaa caccaggtgg aaccatctat catggtgcag attctcactt ctcaaactat
    32761 tttgcaaaac atggtaaaga ctttaaaatt gatgttgctt tgaataacta tggtgaaaat
    32821 ccggtaggta tccaagacaa aatgacatct atcgaccttc ttcgtatggc agaaaatctg
    32881 cgtaccaaag tcattatccc agttcactat gatatctggt ctaacttcat ggcttctact
    32941 aatgagattc tagaactttg gaaaatgcga aaagatcgct tgcaatacga tttccatcca
    33001 tttatctggg aagttggcgg taagtacact tatcctcaag atcaacactt agtagaatac
    33061 catcatccac gtggttttga tgattgtttt gaacaagact ctaacattca atttaaagct
    33121 ttgctataat ataaaaatat tctttggagg ttatccgtat gttgcatgat acggataatt
    33181 ttcatataat agtaaaatag aatgtgtgat tcaataatca cctcaaatag aaaggaaatt
    33241 ctatgtcaaa tctatctgtt aatgcaattc gttttctagg tattgacgcc attaataaag
    33301 ccaactcagg tcatccaggt gtggttatgg gagcggctcc gatggcttac agcctcttta
    33361 caaaacaact tcatatcaat ccagctcaac caaattggat taaccgcgac cgctttattc
    33421 tttcagcagg tcatggttca atgctccttt atgctcttct tcacctttct ggttttgaag
    33481 atgtcagcat gaatgagatt aagagcttcc gtcaatgggg ttcaaaaaca ccaggtcacc
    33541 cagaatttgg tcatacggca gggattgatg ctacgacagg tcctctaggg caagggattt
    33601 caactgctac tggttttgcc caagcagaac gtttcttggc agccaaatat aaccgtgaag
    33661 gctacaatat ctttgaccac tatacttacg ttatctgtgg agacggagac ttgatggaag
    33721 gtgtctcaag cgaggcagct tcatacgcag gtttgcaaaa acttgataag ttggttgttc
    33781 tttatgattc aaatgatatc aacttggatg gtgagacaaa ggattccttt acagaaagtg
    33841 ttcgtgaccg ttacaatgcc tacggttggc atactgcctt ggttgaaaat ggaacagact
    33901 tggaagccat ccatgctgct atcgaaacag caaaagcttc aggcaagcca tctttaattg
    33961 aagtgaagac ggttattgga tatggttctc caaacaaaca aggaactaat gctgtacacg
    34021 gcgcccctct tggagcagat gaaactgcat caactcgcca agccctcggt tgggactacg
    34081 aaccatttga aattccagaa caagtatatg ctgatttcaa agaacatgtt gcagaccgtg
    34141 gcgcatcagc ttatcaagct tggactaaat tagttgcaga ttataaagaa gctcatccag
    34201 aactggctgc agaagtagaa gccatcatcg acggacgtga tccagtcgaa gtgactccag
    34261 cagacttccc agctttagaa aatggttttt ctcaagcaac tcgtaattca agtcaagatg
    34321 ccttgaatgt tgtggcagct aaattaccaa ccttcctagg tggatcagct gaccttgctc
    34381 actcaaacat gacttatatc aagacggatg gacttcaaga cgataccaat cgcttgaatc
    34441 gtaacattca gtttggtgtt cgtgaatttg caatgggaac aatcttgaac gggatggccc
    34501 ttcatggtgg acttcgtgta tacggtggaa ctttcttcgt cttctctgac tatgtgaagg
    34561 cagctgtccg cttgtcagcc ttacaaggac ttcctgtgac ttatgtcttt acccatgatt
    34621 caatcgcagt tggggaagat ggtccgactc acgaaccagt tgagcattta gcaggtcttc
    34681 gtgctatgcc aaatctaaat gttttccgtc cagcagatgc gcgtgaaacg caagcagctt
    34741 ggtaccttgc agtgacaagt gagaaaacac caactgccct tgtcttgaca cgtcaaaact
    34801 tgactgttga agatggaaca gacttcgaca aggttgctaa aggtgcttat gttgtatatg
    34861 aaaatgcagc cgactttgat accatcttga ttgcgacagg ttcagaggtt aatcttgctg
    34921 tctcagctgc taaagaattg gctagtcaag gcgaaaaaat ccgcgtagtc agcatgccat
    34981 ctacagatgt ctttgataaa caagatgcag cttacaagga agagattctt ccaaatgcag
    35041 tccgccgtcg tgttgcagtc gaaatgggtg caagtcaaaa ctggtacaaa tatgttggtc
    35101 tcgatggtgc cgttctaggt attgatacct tcggagcctc tgccccagca ccaaaagtat
    35161 tggcagaata tggctttact gtagaaaatc ttgtaaaagt tgttcgaaac ttgaaataat
    35221 cctaaaaatc agggcgtaag ctctggtttt tcttaccaga aaagtaaggt acaatcttgt
    35281 aaaagtagct gaaatttgat atagtagtcc tatgtaaaag acaaaggaga agacaatgaa
    35341 tccaaatatt acttttttaa tcatgcttgt aggtatgatg gccttgatgt tctttatgca
    35401 acgttctcaa aagaaacaag ctcaaaagcg tatggaaagc ttaaataaac tacaaaaagg
    35461 ctatgaagtg attacaatcg gtggacttta tggaacagtc gatgaagtag atacggagaa
    35521 gggaacaata gttcttgacg tagatggagt ttacttgact tttgaactaa ctgctatcaa
    35581 gacagtatta ccgctgaaag aaacagcttc actagaaggc gcaattgaaa aataagacgg
    35641 gatcacgaac tcccgttttt ctataaaaga aaggaaatgg gatgaaaaaa ttagtctttg
    35701 tctgtctggg aaatatttgc cgtagcccta tggccgagtt tgttatgaaa tcaatgacag
    35761 ataactacga aatccaaagt cgagcaactt cctcttggga acatggcaat ccgattcata
    35821 aggggactca gggaattttt caagagtatg agattcctta tgacaagaac aagacatcgc
    35881 ttcagattag taaggaagat tttgaagcct ttgattatat tatcggaatg gacgcttcaa
    35941 atatttctga cttacgtcag atgtgtccag tagactgtca agataagatt tactcatttt
    36001 catctgaaag tgttccagac ccttggtata caggagattt tgaagaaacc tatcgacgtg
    36061 ttcaagaggg ctgtcaagct tggttagaac gtttagaaaa ggagagttaa ggtggaaaat
    36121 cttacgaatt tttacgaaaa gtatcgtgtc tatctgactc gtccacgttt agagcttttg
    36181 gcagtagtta ccattgtttt ctgtgctgta ctcgtctttt ttctaaatat tccaggaaaa
    36241 ggtgtcttaa aactcgataa tggaacgatt gtttatgatg gcagtcttgt ccgtggtaaa
    36301 atgaatggtc aaggtaccat taccttccaa aatggagacc aatatacagg tggcttcaac
    36361 aatggatcct tcaacggaaa aggtaccttt caatctaaag aaggctggac ctacgaaggt
    36421 gattttgtaa atggtcaggc tgaaggaaaa gggaaactaa caacagaaca agaagtcgtt
    36481 tatgaaggaa cttttaaaca aggcgttttt caacaaaaat aaagcctcct tatccaaagg
    36541 aggtattatt agaaccacaa ggtaagcgct tacctgtaaa tccctttctt tccaaatccc
    36601 tcttccaagc aagtttgtga aataaaaaat atttgaaata aatttcacaa acttcaaaga
    36661 taaaacctga tgagaaaaga aaatgagaaa agtttcacaa gagtttaaaa attctttgtg
    36721 aaatgtttat gaaactgttt acaaagttga aataatgcgt tataataaac ttgtgaaaaa
    36781 attaacaaag gatatcgttc cttgaaagct atggaggaaa atatggctga taaaaaaact
    36841 gtgacaccag aggaaaagaa actcgttgct gaaaaacacg tagatgagtt ggttcaaaaa
    36901 gctctagttg cccttgaaga aatgcgtaaa ttggatcaag agcaagttga ctacatcgtt
    36961 gccaaagcat cagtagcagc tttggatgcc cacggagaat tggctttaca tgcctttgaa
    37021 gaaacaggac gtggtgtatt tgaagataaa gcaactaaga acttgtttgc ctgtgaacac
    37081 gtagtaaaca acatgcgtca cactaagaca gttggcgtta tcgaagaaga cgatgtaaca
    37141 ggattgactc ttattgctga accagttggt gttgtttgtg gtattactcc aacaacaaac
    37201 ccaacatcaa cagcaatctt caaatcattg atttcattga agacacgtaa cccaatcgtc
    37261 tttgccttcc atccatcagc acaagaatca tctgctcatg cagctcgtat cgtccgcgat
    37321 gcagctatcg cagctggtgc tcctgaaaac tgtgtgcaat ggattactca accatctatg
    37381 gaagcaacaa gtgcccttat gaaccacgaa ggtgttgcga caatccttgc aacaggtggt
    37441 aatgccatgg ttaaggcggc ttattcatgt ggtaaaccag ctcttggggt aggtgccgga
    37501 aacgttccag cttatgttga aaaatcagca aacattcgtc aagcagcaca cgatatcgtc
    37561 atgtctaaat catttgataa cggtatggtc tgtgcatctg aacaagcagt tatcattgat
    37621 aaagaaattt acgatgaatt tgtagcagag ttcaaatctt accacactta ctttgtaaac
    37681 aaaaaagaaa aagctcttct tgaagagttc tgcttcggcg tcaaagcaaa cagcaaaaac
    37741 tgtgctggtg caaaattgaa cgctgacatc gttggtaaac cagcaacttg gattgcagaa
    37801 caagcaggat ttacagttcc agaaggaaca aacattcttg ctgcagaatg taaagaagtt
    37861 ggcgaaaatg agccattgac tcgtgaaaaa ttgtcaccag ttattgcagt tttgaaatct
    37921 gaaagccgtg aagatggtat tactaaggct cgtcaaatgg ttgaatttaa cggtcttgga
    37981 cactcagcag ctatccacac agctgacgaa gaattgacta aagaatttgg taaagctgtt
    38041 aaagctattc gtgttatctg taactcacct tctacttttg gtggtatcgg ggacgtttac
    38101 aatgccttct tgccatcatt gacacttgga tgtggttctt acggacgcaa ctcagttggg
    38161 gataacgtta gtgccattaa cctcttgaat atcaaaaaag tcggaagacg gagaaataac
    38221 atgcaatgga tgaaacttcc ttcaaaaaca tactttgaac gtgattcaat tcaatacctt
    38281 caaaaatgtc gtgacgttga acgtgtcatg atcgttactg accatgccat ggtagagctt
    38341 ggtttccttg atcgtatcat cgaacaactg gaccttcgtc gcaataaggt tgtttaccaa
    38401 atctttgcgg atgtagaacc ggatccagat atcacaactg taaaccgtgg tactgagatt
    38461 atgcgtgcct tcaaaccaga taccatcatc gcactcggtg gtgggtctcc aatggatgct
    38521 gccaaagtaa tgtggctctt ctacgagcaa ccagaagtgg acttccgtga ccttgtccaa
    38581 aaattcatgg atatccgtaa acgtgccttc aagttcccat tgcttggtaa gaagactaaa
    38641 ttcatcgcga ttccaactac atctggtaca ggatctgaag taacaccatt tgccgttatc
    38701 tctgataaag caaacaaccg taaataccca atcgctgact actcattgac accaactgtg
    38761 gcaatcgtag atcctgcttt ggtattgaca gttccaggat ttgttgctgc tgatactggt
    38821 atggacgtat tgactcacgc gacagaagca tacgtatcac aaatggctag tgactacact
    38881 gatggtttag cacttcaagc cattaaattg gtctttgaaa atctcgaaag ctcagttaag
    38941 aatgcagact tccactcacg tgagaaaatg cataacgctt caacaatcgc tggtatggcc
    39001 tttgccaatg ccttcctagg tatttctcac tcaatggccc ataagattgg tgcgcaattc
    39061 cacacaatcc acggtcgtac aaatgctatc ttgcttccgt acgttatccg ttacaatggt
    39121 acacgtccag ctaagacagc aacatggcct aagtacaact attaccgtgc agatgaaaaa
    39181 taccaagata tcgcacgcat gcttggactt ccagcttcta ctccagaaga aggggttgaa
    39241 tcttacgcaa aagctgtcta cgaactcggt gaacgtattg ggatccaaat gaattttaga
    39301 gaccaaggaa ttgacgaaaa agaatggaaa gaacattctc gtgaattagc cttcctggct
    39361 tatgaagacc aatgttcacc agctaaccca cgtcttccaa tggtagacca tatgcaagaa
    39421 atcatcgaag atgcatacta tggctacaaa gaaagaccag gacgccgtaa ataattgttt
    39481 atcagtctag aagcaagaca aaaactcaat ttgagggaaa gatccagtaa tttttctatg
    39541 ataaaaggca tcctatcaag gtttttgaat acctgatagg atgccttttt atgatgttgg
    39601 ggcctttttg ccctttttga aaaactagaa tagaaacaaa atatatagca gattgaaact
    39661 agaatagtac acatctgctt ctaaaacatt gttagaattc gatttgattt
//
LOCUS       DAVKZB010000007        36865 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKZB010000007 DAVKZB010000000
VERSION     DAVKZB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.007"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(141..1076)
                     /locus_tag="VXA80_000338"
     CDS             complement(141..1076)
                     /locus_tag="VXA80_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV0352887.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1073..1933)
                     /locus_tag="VXA80_000339"
     CDS             complement(1073..1933)
                     /locus_tag="VXA80_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0352888.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIS
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQSPSHTRKEYS
                     QYQLVRKATL"
     gene            2041..4203
                     /locus_tag="VXA80_000340"
     CDS             2041..4203
                     /locus_tag="VXA80_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0352889.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            4238..5497
                     /locus_tag="VXA80_000341"
     CDS             4238..5497
                     /locus_tag="VXA80_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0352890.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDTIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5511..6377
                     /locus_tag="VXA80_000342"
     CDS             5511..6377
                     /locus_tag="VXA80_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0352891.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6391..7224
                     /locus_tag="VXA80_000343"
     CDS             6391..7224
                     /locus_tag="VXA80_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0352892.1"
                     /translation="MKQDERKALIGKYTLLILGTVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7535..8977
                     /gene="gtfA"
                     /locus_tag="VXA80_000344"
     CDS             7535..8977
                     /gene="gtfA"
                     /locus_tag="VXA80_000344"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV0352893.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9115..10308
                     /locus_tag="VXA80_000345"
     CDS             9115..10308
                     /locus_tag="VXA80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV0352894.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMNSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10993..12972
                     /locus_tag="VXA80_000346"
     CDS             10993..12972
                     /locus_tag="VXA80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0352895.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13039..14535
                     /locus_tag="VXA80_000347"
     CDS             13039..14535
                     /locus_tag="VXA80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352896.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14535..15461
                     /locus_tag="VXA80_000348"
     CDS             14535..15461
                     /locus_tag="VXA80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352897.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15470..16537
                     /locus_tag="VXA80_000349"
     CDS             15470..16537
                     /locus_tag="VXA80_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352898.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16548..17474
                     /locus_tag="VXA80_000350"
     CDS             16548..17474
                     /locus_tag="VXA80_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352899.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18042..18902
                     /locus_tag="VXA80_000351"
     CDS             18042..18902
                     /locus_tag="VXA80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0352900.1"
                     /translation="MSQFITHQSLDATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTAPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQVNLIKKVLPKAKTIGILYTQSEPNSVVQKDEDKRLLKEKGFT
                     VVEKTILDSNNVKAAAENLMAEVDMVFVPTDNIISSTMETVKQVSIKHKVPVFGGSTE
                     MIAVGDLYNYGTNYEELGRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            18914..19803
                     /locus_tag="VXA80_000352"
                     /pseudo
     CDS             18914..19803
                     /locus_tag="VXA80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19816..20619
                     /locus_tag="VXA80_000353"
     CDS             19816..20619
                     /locus_tag="VXA80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0352901.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASSLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20652..21362)
                     /gene="treR"
                     /locus_tag="VXA80_000354"
     CDS             complement(20652..21362)
                     /gene="treR"
                     /locus_tag="VXA80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV0352902.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21547..23514
                     /gene="treP"
                     /locus_tag="VXA80_000355"
     CDS             21547..23514
                     /gene="treP"
                     /locus_tag="VXA80_000355"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV0352903.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23692..25317
                     /gene="treC"
                     /locus_tag="VXA80_000356"
     CDS             23692..25317
                     /gene="treC"
                     /locus_tag="VXA80_000356"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV0352904.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25573..25821
                     /locus_tag="VXA80_000357"
     CDS             25573..25821
                     /locus_tag="VXA80_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV0352905.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26028..26822
                     /gene="racE"
                     /locus_tag="VXA80_000358"
     CDS             26028..26822
                     /gene="racE"
                     /locus_tag="VXA80_000358"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV0352906.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26819..27790
                     /locus_tag="VXA80_000359"
     CDS             26819..27790
                     /locus_tag="VXA80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV0352907.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27766..28287
                     /locus_tag="VXA80_000360"
     CDS             27766..28287
                     /locus_tag="VXA80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0352908.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28284..28745
                     /locus_tag="VXA80_000361"
     CDS             28284..28745
                     /locus_tag="VXA80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0352909.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28736..29476
                     /gene="xerD"
                     /locus_tag="VXA80_000362"
     CDS             28736..29476
                     /gene="xerD"
                     /locus_tag="VXA80_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV0352910.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29476..30210
                     /locus_tag="VXA80_000363"
     CDS             29476..30210
                     /locus_tag="VXA80_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV0352911.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQSST"
     gene            30223..30792
                     /locus_tag="VXA80_000364"
     CDS             30223..30792
                     /locus_tag="VXA80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV0352912.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30779..31501
                     /locus_tag="VXA80_000365"
     CDS             30779..31501
                     /locus_tag="VXA80_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0352913.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31501..31743
                     /gene="yidD"
                     /locus_tag="VXA80_000366"
     CDS             31501..31743
                     /gene="yidD"
                     /locus_tag="VXA80_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV0352914.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(31968..32933)
                     /locus_tag="VXA80_000367"
     CDS             complement(31968..32933)
                     /locus_tag="VXA80_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0352915.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33029..33781)
                     /locus_tag="VXA80_000368"
     CDS             complement(33029..33781)
                     /locus_tag="VXA80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0352916.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33778..34734)
                     /locus_tag="VXA80_000369"
     CDS             complement(33778..34734)
                     /locus_tag="VXA80_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV0352917.1"
                     /translation="MQTKSKHTKLFWILIILTIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(34724..35683)
                     /locus_tag="VXA80_000370"
     CDS             complement(34724..35683)
                     /locus_tag="VXA80_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352918.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36106..36808)
                     /gene="galE"
                     /locus_tag="VXA80_000371"
                     /pseudo
     CDS             complement(36106..36808)
                     /gene="galE"
                     /locus_tag="VXA80_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
BASE COUNT        10662 a         6884 c         8190 g        11129 t
ORIGIN      
        1 aaaaataggc tccataatat ctatagggga tttacccact acaaatatta tagagacaat
       61 aaaacttcag atccgtaaaa cttaattgaa ttactaatct gaagttattt ttatatcgtt
      121 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
      181 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      241 gcaagtccct tgtagtcttt ttgctctagt gtgaaagtga cttcttttcc tagaatgaat
      301 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      361 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      421 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      481 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      541 ttatgattgt ttagatagat atcattgacc cattttatat cgacatctat taaagttagg
      601 ttcttaatgg ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      661 taggggagat ttggtttaag atggagtgtc atataaattc caccttgtgg tgagtagaag
      721 gaacgttgaa aacggcctcg gcctgctgtt tgataggaag ctagatagag agtatttgct
      781 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      841 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      901 attagtttat atcctctatt tttgatacta ttaatttcaa tgccttcttg ttctagtcgc
      961 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
     1021 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
     1081 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagactgat
     1141 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1201 caattacctt gatggactct tgggtatttt cgagaagttg tctagctcgg tgcattcgaa
     1261 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1321 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1381 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1441 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1501 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1561 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1621 aaatttgaga aagagcaaaa taatcagggg atttccctcc agtgattcct aaccagtagt
     1681 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1741 agaaatctcc ttcttttaaa tcaacaattt tacccttgta atgaaatttt ccttgtcctt
     1801 tactaatgta atgtaggacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1861 tgcattcctc atatccataa aagcttaggg caaggtcaat tgttccagtc tggtattctg
     1921 aaaaaactag catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1981 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     2041 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     2101 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2161 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2221 aatcgaacct ttagtttaga tactcagcga cagatttttg gacaacatgg cttaggagat
     2281 tttaggaaac caaccataca ggttcagcat agtgtaactg aagtaacaga ctttcgattt
     2341 gtagaagcaa agattttaaa aggtcagaat ggtccacagg gcttaccttc tccacatagc
     2401 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2461 actttgtatt atactacttt taataatgat gcgactattg ctagctacag taaattagat
     2521 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2581 gctgcagatt acgaaatagt aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2641 cgtcaacagg tagaacaagg aatcttttcg attagctcaa accgaggtgc ttctggtcat
     2701 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2761 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2821 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2881 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2941 attagtcatg aaagtcagaa ttttgtactg aagcacatta ttctaagtga attttctaaa
     3001 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     3061 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3121 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3181 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3241 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3301 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3361 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3421 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3481 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctgggactt
     3541 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3601 ggtggtggtg gacgaaatga tcttggtatg atgcgctatt tcccacaagt ctgggctagt
     3661 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3721 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3781 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3841 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3901 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3961 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     4021 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     4081 ctatatgaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4141 ctaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaagacta
     4201 tgataagggt gtataaattt ataaaggagg ctttctaatg gaatggtata aaaaaatcgg
     4261 acttcttgca actacaggtt tagctttgtt tgggctcggc gcttgctcca actatggtaa
     4321 atctgcggat gacacagtga ccatcgagta tttcaaccag aaaaaagaaa tgaccaaaac
     4381 cttggaagaa atcactcgtg attttgagaa ggaaaaccct aagatcaagg tcaaagtcgt
     4441 caatgtacca aatgctggtg aagtattgaa gacacgcgtt ctcgcaggag atgtgcctga
     4501 tgtggtcaat atttacccac agtccatcga actgcaagaa tgggcaaaag caggtgtctt
     4561 tgaagatttg agcaacaaag actacctgaa acgcgtgaaa aatggctacg ctgaaaaata
     4621 tgctgtaaac gaaaaagttt acaacgttcc ttttacagct aatgcttatg gaatttacta
     4681 caacaaagat aaattcgaag aactgggctt gaaggttcct gaaacctggg atgaatttga
     4741 acagttagtc aaagatatcg ttgctaaagg acaaacacca tttggaattg caggtgcaga
     4801 tgcttggaca ctcaatggtt acaatcaatt agcctttgcg acagcaacag gtggaggaaa
     4861 agaagcaaat caataccttc gttattctca accaaatgcc attaaattgt cggatacgat
     4921 tatgaaagat gatatcaagg tcatggacat ccttcgcatc aatggctcta agcaaaagaa
     4981 ctgggaaggt gctggctata ccgatgttat cggagccttc gcacgtgggg atgtcctcat
     5041 gacaccaaat gggtcttggg cgatcacagc gattaatgaa caaaaaccga actttaagat
     5101 tgggaccttc atgattccag gaaaagaaaa aggacaaagc ttaaccgttg gtgcgggaga
     5161 cttggcatgg tctatctcag ccaccaccaa acatccaaaa gaagccaatg cctttgtgga
     5221 atatatgacc cgtccagaag tcatgcaaaa atactacgat gtggacggat ctccaacagc
     5281 gatcgaaggg gtcaaacaag caggagaaga ttcaccgctt gctggtatga ccgaatatgc
     5341 ctttacggat cgtcacttgg tctggttgca acaatactgg accagtgaag cagacttcca
     5401 taccttgacc atgaactatg tcttgaccgg tgataaacaa ggcatggtca atgatttgaa
     5461 tgccttcttt aacccgatga aagcggatgt ggattaggag tagagagact atgaaaaaag
     5521 tattacaaaa atattgggca tgggcttttg tggtcatccc cctcttgtta caagcaattt
     5581 tcttctatgt gccgatgttt caaggagcct tttacagttt taccaactgg acaggattga
     5641 cttacaacta caaatttgtc ggcttaaaca actttaagct cctcttcatg gatccaaaat
     5701 tcatgaatgc gattggcttt accgcaatca ttacgattgc catggtggtt ggtgagattg
     5761 cactcgggat cttcattgcg cgtgtcttga actctaaaat caaaggccaa accttcttcc
     5821 gtgcttggtt cttcttccca gctgttttat ctggtttgac agtggctttg atcttcaagc
     5881 aagtcttcaa ctacggtctt ccagcgattg ggaatgccct tcatattgaa tttctccaaa
     5941 ccagtctttt agggactaag tggggagcaa tctttgcggc tgtctttgtc cttctttggc
     6001 aaggggtggc tatgcccatc atcatcttcc tagctggttt gcaatctatt ccaactgaga
     6061 ttacagaggc agcaaggatt gatggtgcga ctagcaagca agttttctgg aacattgaat
     6121 tgccttactt gctaccaagt gtctctatag tctttatcct agccctaaaa ggtgggctga
     6181 ctgcctttga ccaagtcttt gccatgaccg gtggtggtcc aaacaatgcc acaacctcac
     6241 ttgggctctt ggtttataac tatgccttta aaaacaacca attcggttat gccaatgcca
     6301 ttgccgtaat cttgttcttc ttaattgtag tgatttcgat catccaattg agagtatcta
     6361 agaaatttga aatttaagag gagaagcatg atgaaacaag atgaaagaaa agccctgatt
     6421 ggcaaataca ctctattgat tctaggaacg gttctgattt tagtgccgct ccttgctacc
     6481 ctctttagtt ccttcaaacc cactaaggat attgtagata atttctttgg atttccaacc
     6541 aacttcacat gggacaactt tagccgtctc ttagctgatg ggattggagg ctattattgg
     6601 aactctgtcg tcatcactgt cttgtcttta cttgcagtaa tgatctttat ccctatggca
     6661 gcctactcca tcgctcgcaa tatgagtaaa agaaaagcct ttaccatcat gtataccctc
     6721 ttaatcctcg gaatcttcgt acctttccaa gtcatcatga ttccgattac ggttatgatg
     6781 agtaaactcg gtttggctaa tacctttggt ttgatcttgc tctacttgac ctatgcgatt
     6841 ccacagaccc tctttctcta tgttggctat atcaaaatct cgattccaga aagtctggat
     6901 gaagcagcag agatcgatgg ggctaatcaa tttacaacct atttccgcat catcttccca
     6961 atgatgaaac cgatgcatgc gacaaccatg atcatcaatg ccctttggtt ctggaatgac
     7021 ttcatgttgc cactccttgt cttgaaccgg gattccaaaa tgtggactct gcctttgttc
     7081 caatacaact acgcaggcca atatttcaac gactacggac caagctttgc ctcttacgtg
     7141 gtcggcatta tcagtatcac cattgtctat ctcttcttcc aacgccatat catttcagga
     7201 atgagcaacg gggcagtgaa gtaatactca atgaaaatca aagagcaaac taggaagcta
     7261 gccgcaggct gtacttgagt acggcaaggt gaagctgacg tggtttgaag agattttcga
     7321 agagtataag aagagctcat ggaatctata gtctttgaca cagaggaaga gttcagcttg
     7381 ttgataattc tatgtagaaa acaagaaagt agtactttgt ttatcacagt ctatattagt
     7441 acgagtttga tgataaatag agatttaaaa tgaatttgat ttacgaagcc agatcatatg
     7501 agacttggct tcaattagaa aaaggagagt aatgatgccg attcaaaata aaaccatgtt
     7561 gattacctat tctgatagcc ttggaaataa tcttaaagac ttatatgata atttggaaga
     7621 gcattttgga gatgctattg gaggagttca ccttttacca tttttcccat caacaggtga
     7681 tcgtggattt gcgccagttg actacgacga agtggactca gcttttggtg attgggagga
     7741 tgttaagcgt ttaggtgaga aatattatct tatgtttgat tttatgatta atcatatttc
     7801 tcgtcaatcc aagtattata aggactatca agaaaaacat gaagccagtg aatttaaaga
     7861 tctcttttta aactgggata agttttggcc agaaaaccgt ccgacacagt ctgatgtaga
     7921 tttaatttac aagcgtaagg atcgtgcacc aaagcaagag attgtgtttg aagatggttc
     7981 agtggaacat ttgtggaata cctttggtga ggagcagatt gatcttgatg tgaccaaaga
     8041 agtaactatg gaatttatcc gtaagaccat tcagcacttg gcaagtaatg ggtgtgattt
     8101 gattcgtcta gatgcctttg cttatgcagt gaagaaattg gatactaatg atttctttgt
     8161 ggaaccagat atttgggatt tattggacaa agttcgagat atcgctgctg agtatgggac
     8221 agagctttta cctgagattc atgaacacta ttcgattcag tttaaaatag cagaccatga
     8281 ttactatgtt tatgattttg ctcttccaat ggtgacactt tatactcttt acagttccag
     8341 aacagagcgt ttggctaagt ggttaaagat gagcccgatg aagcaattta cgacgctaga
     8401 tacccatgat gggattggag tagtagatgt caaggatatc ctgaccgatg aggagattga
     8461 ctatgcttca aatgaactct ataaggttgg agctaatgtc aaacgtaagt actctagtgc
     8521 cgagtataac aacttagata tctaccaaat caattcaacc tactattcag cgcttggaga
     8581 tgatgatgtc aagtattttc tcgctcgtct aattcaagct tttgccccag gtattcctca
     8641 ggtttactat gtgggtctat tagcaggcaa gaatgacttg aaattattag aagaaactaa
     8701 agaaggtcga aatattaatc gtcattacta tagcaacgag gaaatagcaa aagaagtgca
     8761 acgacctgtt gtgaaggctc ttctcaatct attttctttc cgtaaccgtt cagaagcctt
     8821 tgatctagaa gggactactg agatagagac accaacagct cacagcattg taatcaaacg
     8881 tcaaaataaa gataagtccg taacagcagt agcagaaatt gatttgcaaa atcagactta
     8941 tcgggtaatt gagaatggag ttgaagtaac attttgaagc cttgatatgg tggataaaat
     9001 agggttttta ttttggaaaa cgtttccttt gttttcaaat tgctaaaaaa gtggtacaat
     9061 ataggatagc ttactattat ctgaatcagc tgatttggag agaaaggatt cattttgaaa
     9121 tcaataggct ttattgaaaa gctgaagggg ttgtctagta aagagctgat tttattggga
     9181 attatcctga gtatcttttt acccttttat ctttttgtag ttgtactctg tttatatatt
     9241 atcagtttga tttttacagg agacatgaaa agtattcttc agaaaatggg ggagcatccg
     9301 atgctgcttc tttttcttag ctatagtact gttatatcca ttcttgcaca aaattggatg
     9361 ggtcttgtgg cttcagtagg aatgtttcta tttactattt tctttttgca ctatcagtcg
     9421 attttatccc ataaattctt tcgattgatt ttgcagtttg tcttgtttgg tagtgtcttg
     9481 tcagctgctt ttgccagttt agaacatttc caaattgtga agaaatttaa ctatgctttt
     9541 ctttcaccca atatgcaggt gtggcatcag aaccgggcag aagtgacctt ctttaatcct
     9601 aattattatg gaattatttg ttgtttctgt attatgattg ctttctatct gtttacaacg
     9661 accaagttga attggttgaa agtattctgt gtgattgcag gctttgttaa tctctttggt
     9721 ttgaacttta ctcaaaatcg aactgccttt cctgctatta tcgctggagc aattatctat
     9781 ctctttacga ctattaaaaa ctggaaggcc ttttggctta gtattggggt cttcgcgatt
     9841 ggtttgagtt tcctcttttc tagtgatttg ggagttcgaa tgggtacttt agactcttct
     9901 atggaagaac gcatttctat ctgggatgct gggatggcct tgtttaagca aaatcctttt
     9961 tggggtgaag ggccattgac ctatatgaac tcttatcctc ggatacatgc tccttatcat
    10021 gaacatgccc acagtcttta tattgatacg attctgagtt acggaattgt ggggactatt
    10081 ttattagttt tgtcttctgt tgctcctgtt cgcttgatga tggatatgag tcaggagtcg
    10141 gggaaacgtc cgattatcgg cctttatcta tctttcctta cagtggttgc tgtgcacgga
    10201 atttttgact tggctctctt ctggattcag tcaggcttta ttttcttgct agttatgtgc
    10261 agtattccat tggagcatcg aatgttggta tcggacatga cggattaagt tttataagtt
    10321 tgaaatcttc taccttgggt aggagatttt ttatacgatg ggcatgtcgt gcatctgagg
    10381 tgtaagtcct ctgtagggct ccactaccgg tgaatacaat agcgctcccc aagcctgact
    10441 atcgtgaggt agcggattaa aatggtctgg ggatagatcg ttttaagtct gacgcaggaa
    10501 ataagaattg tcagagaaag caatagtgaa atcgtaggct atgaatagaa acgtgatagc
    10561 gaggagcata tagtggataa ggtagcgaga aattttaaag tataaaagat agtcttaatc
    10621 tatatatgta aatcacaaaa gtaattgaat tcgggataag gttggtgtga aaagacatag
    10681 taaactgaat cttgaatact agaatgatac ttctacaata ttgttataat ttaatttgaa
    10741 ttccaaaatc acattgttcc gtccgtattt cattttactg tatgtcttcc taaactccaa
    10801 agtttctgcg tcaaatttcc tattttcact gtagtctttg acacttcgaa cgacaatttg
    10861 aatcatatat tcaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat tctgaaaatt
    10921 tgtttgattt ttaatggata atgtgatata atggttgtca atctataatt cacataaaca
    10981 gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt tttattagca
    11041 gcaggtgtac ttgcagcatg cagttcttca aaatcatctg attcatcagc ccctaaagct
    11101 tatggctatg tttatacagc agacccagaa accttggact acctgatttc aagtaaaaat
    11161 agtacaacag tagtgacttc aaatgggatt gatggtttat tcactaacga taattacggt
    11221 aatcttgctc ctgcagttgc agaggattgg gaagtctcta aggatggttt gacctacact
    11281 tataagattc gtaaaggggt taaatggttt acctctgatg gagaagaata tgcagaggtg
    11341 acggctaaag atttcgtgaa cggtttaaaa cacgcagcag ataaaaaatc agaagctatg
    11401 tatttagctg aaaattcggt taaaggcttg gcagattatc tatcaggaac ttcaacagat
    11461 ttttcaacag ttggtgtcaa ggcggttgat gattatacgt tacaatacac tttgaaccag
    11521 cctgaaccgt tctggaactc taagttgacc tattctattt tctggcctct gaatgaagaa
    11581 ttcgaaacat caaaaggaag cgattttgct aaaccaacag atccgacatc cttgctttat
    11641 aatggtccat tcttgttgaa agggttgact gcaaaatctt ctgtagagtt tgtaaaaaat
    11701 gagcaatatt gggataaaga aaatgtccac ctagatacta tcaatctagc ttactatgat
    11761 ggatcagatc aggagtcgct agagcgtaac ttcactagtg gagcttatag ttatgcccgt
    11821 ctttacccta ccagctccaa ctattctaag gttgcagaag aatacaagga caatatctat
    11881 tacacacaat caggctctgg gattgctggt ctgggtgtga atattgatcg ccaaagttac
    11941 aactatactt ctaaaactac agattcagag aaagtagcta ctaagaaggc attgcttaac
    12001 aaagatttcc gtcaagcctt gaattttgct cttgatcgct cagcttactc agctcaaatc
    12061 aatggtaaag atggagcagc tttagcagtt cgtaatttat ttgtaaaacc agactttgtt
    12121 tcagctggtg agaagacctt tggtgattta gtcgctgctc aacttcctgc ttatggtgat
    12181 gagtggaaag gtgtgaattt agctgatggg caggatggtt tattcaatgc tgacaaggcc
    12241 aaggcagagt ttgcgaaagc taagaaagct ttagaagcag acggcgttca gtttcctatt
    12301 catctggacg ttccagtaga ccaagcatca aaaaactaca tatctcgtat tcagtccttt
    12361 aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat tcaacaaatg
    12421 acaagtgatg aattccttaa tattacttac tatgctgcca atgcttcatc tgaggattgg
    12481 gatgtatcag gaggagtttc atggggtcca gactatcaag acccatctac ttacctggat
    12541 attttaaaaa caactagcag tgaaactaca aaaacatatt taggatttga taatccaaat
    12601 agcccttcag tagttcaagt tggtttgaaa gaatacgata aattagttga tgaagctgcc
    12661 agagagacaa gcgacttgaa tgtccgttat gaaaaatatg cagcggctca agcatggttg
    12721 acagatagtt cactctttat tcctgctatg gcttcttctg gtgcagcacc agtgctttca
    12781 cgaattgttc catttactgg agcttctgcg caaacaggct ctaaggggtc agatgtttac
    12841 ttcaaatatt tgaaattaca agataaagtg gtgactaagg aagagtatga aaaagctcgt
    12901 gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga attggcaagt
    12961 catgtgaagt aaataatttc taatagaact ttctctccat gaggagaagg ttcttttggg
    13021 atttttaaag gaaataatat gaaaaaatat atttttatgc gtgttttgcg gtcattggtt
    13081 tcgattttct tagtaacgac tttgacctac acgattatct ataccttggt tcctcgaaaa
    13141 ttgattttca agcaggatcc taactataat aaaattgcga caacggctga taaacgtgat
    13201 aactatgaaa atactgtgtt tgagcgtatg ggctacattg agtattacga tactaaagag
    13261 ttgcaagaaa aggcaagtag catggattct tctgtaacag tagaagcaaa tgcgaccaat
    13321 aaagctattt atgaaaagta catcaatcaa ttaggtcatg gttggacttt gggagaattt
    13381 actgaaagtg gtcaattcta tgctactcgt gaaattccaa tttttgaacg tgtttttcac
    13441 ttctatgcta acttgattga cattgaccat acaaataaaa tccaagaccc tgaaaatcca
    13501 gacttgaaac gctaccttcg ttttgaaaat gatccagcta tcggatggtc attggtcggt
    13561 tcaggaacta aacataaata tctcttgtac tttaacagtc agttcccatt tgttcatcaa
    13621 aactttgtga acttgaattt aggtgactct tacccaacct atgctaatac accagttctt
    13681 caggttatta ctcaaggtca aggacaaacc aaaactgccc aagttcagtt cccaacaggt
    13741 aagaaaacgt cttctgtaaa tatttactca agaacctaca agtcacctag tcaggctgac
    13801 tctcgtgaag tagctagcta tgggaaagat gatccttata cagcgactga aagtaattac
    13861 caatatccat ctatgattgt cagctctgct attactggtt tgattggttt ggtgcttgcc
    13921 tatgctcttg ccgtgccact tggttcagcc atggctcgtt tcaagaacac ttggattgat
    13981 agcctctcaa caggggcttt gaccttcttg cttgctcttc caacgattgc cttggtttac
    14041 atcgttcgat tgattggatc atctatttcc cttccagatt cattccctat cttgggagct
    14101 ggagattggc gttcttacgt tttaccagca gtcatccttg gtttgttggg tgctcctggt
    14161 acagccattt ggattcgtcg ttacatgatt gacttgcaat ctcaagactt tgttcgtttc
    14221 gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt taaaaatgcc
    14281 atggttccgc tggtttcagg aattcctggt gccattattg gggttatcgg tggtgcaacc
    14341 cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa tgttgattga ctctgtaaaa
    14401 gcatctaata actctatggt cgttggtctt gtcttcatct ttacatgtat ttctatcttc
    14461 tcacgtcttt tgggagatat ttggatgact attattgacc cacgtattaa attgactgag
    14521 aaaggaggca aataatgtct acaatcgata aagaaaaatt tcagtttgta aaacgtgacg
    14581 attttgcctc tgaaactatt gatgcgccag catattctta ctggaaatca gtgtttaaac
    14641 aatttatgaa gaaaaaatca actgtagtca tgttgggaat cttggtagcc atcattttga
    14701 taagtttcat ctacccaatg ttttctaagt ttgatttcaa tgatgtcagc aaggtaaacg
    14761 actttagtgt tcgttatatc aagccaaatg cggagcattg gttcggtact gacagtaacg
    14821 gtaaatcgct ctttgacggt gtctggttcg gagctcgtaa ctccatcctc atttctgtga
    14881 ttgcgacagt gattaacttg gttatcggtg tttttgtcgg tggtatttgg ggtatttcaa
    14941 aatcagttga ccgtgtcatg atggaagttt acaacgtcat ctcaaacatc ccacctcttt
    15001 tgattgttat tgtcttgact tactcaatcg gagctggatt ctggaatctg atttttgcca
    15061 tgagcgtaac aacatggatt ggtattgcct tcatgatccg tgtgcaaatc ttgcgctatc
    15121 gtgacttgga atacaacttg gcgtcacgta ctttgggaac accaaccttg aagattgttg
    15181 ccaaaaatat catgcctcaa ttggtatctg ttattgtgac aaccatgact caaatgcttc
    15241 caagctttat ctcatacgaa gccttcttgt ctttcttcgg tcttggatta ccgattacag
    15301 tgccaagttt gggtcgtttg atttcggatt attcacaaaa cgtaacaacc aatgcttact
    15361 tgttctggat tccattgaca acccttgtct tggtatcctt gtcccttttc gtagttggtc
    15421 aaaacttagc ggatgctagt gatccacgta cacatagata ggagtagaaa tgacaaaaga
    15481 aaaaaatgta attttgactg ctcgcgatat tgtcgtggaa tttgacgttc gtgacaaagt
    15541 attgacagcc attcgcggcg tttcccttga actagtcgaa ggagaagtat tagccttggt
    15601 aggtgagtca ggatcaggta aatctgtttt gacaaagacc ttcacaggta tgctcgaaga
    15661 aaatggtcgc attgcccaag gtagtattga ctaccgtggt caggacttga cagctttatc
    15721 ttctcacaag gattgggaac aaattcgtgg tgctaagatt gcgactatct tccaggaccc
    15781 aatgactagt ttggacccca ttaaaacaat tggtagtcag attacagaag ttattgtaaa
    15841 acaccaagga aaaacagcta aagaagcgaa agaattggcc attgactaca tgaataaggt
    15901 tggcattcca gacgcagata gacgttttaa tgaataccca ttccaatatt ctggaggaat
    15961 gcgtcaacgt atcgttattg cgattgccct tgcctgccga cctgatgtct tgatctgtga
    16021 tgagccaaca actgccttgg atgtaactat tcaagctcag attattgatt tgctaaaatc
    16081 tttacaaaac gagtatcatt tcacaacaat ctttattacc cacgaccttg gtgtggtggc
    16141 aagtattgcg gataaggtag cggttatgta tgcaggagaa atcgttgagt atggaacggt
    16201 tgaggaagtc ttctatgacc ctcgccatcc atatacatgg agtctcttgt ctagcttgcc
    16261 tcagcttgct gatgataaag gggatcttta ctcaatccca ggaacacctc cgtcacttta
    16321 tactgacctg aaaggggatg cttttgcctt gcgttctgac tacgcaatgc agattgactt
    16381 cgaacaaaaa gctcctcaat tctcagtatc agagacacat tgggctaaaa cttggcttct
    16441 tcatgaggat gctccaaaag tagaaaaacc agctgtgatt gcaaatctcc atgataagat
    16501 ccgtgaaaaa atgggatttg cccatctggc tgactaggag gaaggaaatg tctgaaaaat
    16561 tagtagaaat caaagattta gaaatttcct tcggtgaagg aagtaagaag tttgtcgcgg
    16621 ttaaaaatgc taacttcttt atcaacaagg gagaaacttt ctcgcttgta ggtgagtccg
    16681 gtagtgggaa aacaactatt ggtcgtgcta tcatcggtct aaatgataca agtaatggag
    16741 atatcatttt tgatggtcaa aagattaatg gtaagaaatc gcgtgaacaa gctgcggaat
    16801 tgattcgtcg aatccagatg attttccaag accctgccgc aagtttgaat gaacgtgcga
    16861 ctgttgatta tattatttct gaaggtcttt acaatcaccg tttatttaag gatgaagaag
    16921 aacgtaaaga gaaagttcaa aatattatcc gtgaagtagg tcttcttgct gagcacttga
    16981 ctcgttaccc tcatgaattc tcaggcggtc aacgtcaacg tatcggtatt gcccgtgcct
    17041 tggtcatgca accagacttt gttattgcag atgagccaat ttcagccttg gacgtttctg
    17101 tacgtgccca agtcttgaac ttgctcaaaa aattccaaaa agagctcggc ttgacctatc
    17161 tcttcatcgc ccatgacttg tcggttgttc gctttatttc agatcgtatc gcagttattt
    17221 acaagggtgt tattgtagag gttgcagaaa cagaagaatt gtttaacaat ccaattcacc
    17281 catatactca agccttgctt tcagcggtac caatcccaga tccaatcttg gaacgtaaga
    17341 aggtcttgaa ggtttacgac ccaagtcaac acgattatga gactgataag ccatctatgg
    17401 tagaaatccg tccaggtcac tatgtttggg cgaaccaagc cgaattggca cgttatcaaa
    17461 aaggactaaa ctagtaatgg ttttataatt tccatgtcaa tctttatgga aattataaaa
    17521 ctttttttgt tggactgatt tgaaaaactt tgaaagcttc ctgaaagaac tttcgaaaaa
    17581 ttttaaaaaa ttctgaaata ttattgacag gtgatgaaaa aggtggtagg ataggaagga
    17641 tcaaaaaaga aagcagaaaa agaaatgaat aagagacaag ccgtaactat gaatcaaaac
    17701 ttttacttta gctactttag atagtgtttg tagacatgtc accagggatg caaacagttc
    17761 aagcaatttg tttgtatcta tgtggttaag atttttgatt gtggtccata tagatgaata
    17821 tctaaatgga ctagctaagt tgtaacttgt taggttattt caagtatccg tttagatagt
    17881 atctaggcgg ttttttgttt tatcttttct gagaaggagt ttcattatga aagttgttcg
    17941 aaaatcacta gccccctctt ggtagtgggg ctcctcttga cttctctgat cagtttgcat
    18001 cagttgaagg cagataagaa aaagatgtct tttgtatcgg tatttcgcag tttattaccc
    18061 accagtcctt agacgctact agagaaggat ttgtggatga gctggccaaa caaggctatg
    18121 ttgaagggga aaatattgag attgatttgc aaaatgcaca gggagaacaa agaaatctaa
    18181 aaacgatttc ccagcaacta gcagaatcca gcgatgtcgt tctagccatc gcaaggcctt
    18241 ctgctcagag cttggccaat acgacgcaaa cgacaccagt tatcttttca gctgtaacag
    18301 ccccagtcag tgccaagttg gttgagtcaa gagaacatcc tgggggcaat gtaactggga
    18361 caagcgatca gtcatcagat gccatttcaa cccaagtcaa tttgataaag aaagtgttgc
    18421 caaaggctaa aacgattgga atcctctata ctcagagcga gcccaattcg gttgttcaaa
    18481 aggatgaaga taagcgactt ctgaaagaaa aaggctttac cgttgttgaa aaaacaatct
    18541 tggacagtaa taacgtcaag gcggcagcag agaatttgat ggcagaggtg gatatggttt
    18601 ttgtcccaac tgataatatc atttcgtcaa ccatggaaac agttaagcag gtttctatta
    18661 aacacaaggt tccagtattc ggtggttcaa cagaaatgat tgctgttggt gacttgtata
    18721 actacggaac caattatgaa gaattgggaa gacagacagc tcgcatgctg attcgtgttt
    18781 taaaaggtga gaaaccagaa aatatagcag ttgagttgcc tgaaaaactg gaattgcata
    18841 ctaatcaaga aatggcagat gcattgggaa ttgatattag taagttagaa ggcaaggaat
    18901 aaggaggatt aagatgaatt ttgtgttatc tagtttatca gaaggtttgt tgtggtcgat
    18961 tgtggcgatt ggggtctact tgactttccg tattttggat attgcggata tgactgctga
    19021 gggagccttt cctctggggg cagcagtcgt tgtatcacag atacaggcag gggcaaatcc
    19081 ttggcttgcg attttacttg ctttgctggc aggtatggta gcaggtcttg tatcaggaat
    19141 gctccacacc aagatgaaaa tcccagctct cttgacaggg attgtgacct tgacagggct
    19201 ctattcaatc aatattaaaa tcatgggcag tgtactcaat ctttccttgg gagattctgc
    19261 aaccgtcttt aaacagttgg cgagcttggg tctgacaaat gaaggagctg ttttctcact
    19321 tagtttagtc tgtctcttac ttgtttggtc ttgacccttt tgatgaaaac agagattggt
    19381 ttggtcttgc gttcgactgg ggataatatc ccgataagtg aggccaatgg agtcaatgta
    19441 gacaccatga agattgttgg ttacatgatt tcaaacggtt tgattgctct atgtggttcc
    19501 ttgtttgccc aaaatgatgg attttcagat gtgacttctg ggacaggaac cattgttgtt
    19561 ggtttgagct cggtgattat tgcggaagtc ttgatacacg acttgaccat tggaggccgc
    19621 ttattatcca tcggaatcgg tgctattgtt taccgtttga ttattttaaa tatctatgaa
    19681 attccaaatc tagatcaaaa cttggtacgt ctctttaatg caatcttgct cactttggtt
    19741 ttatttgcac cagaattgca aaagagatta aagattcgtg gtctgaaatt gagaaatgaa
    19801 taggaggaga aagttatggc aagtttgtta acacttgaaa atattcacaa gacatttgaa
    19861 gcagggacgg tcaatgagaa ccatgtcctc aaaggattag atttagaggt tgaagaggga
    19921 gattttatct ctgtgattgg tggaaatgga gcaggaaaat ccactttgat gaatatcttg
    19981 gctggcaatc tatcggtgga cgaaggggac cttctactgg cagggaaatc cattaaaaat
    20041 ctgagtgtta gaaagagagc caaggatatt gcccgtgttt ttcaagatcc taagatggga
    20101 acagcttcta gtttgacgat tgaggagaat atggctattg ccttgagacg tgggcaaaag
    20161 agagggcttg gttggggcgt gaaggagaag gatagaatcc agttccaaga ggccttgaag
    20221 gagttgaata ttggtcttga aaaccgctta aaagtagata ctcaatatct ctcaggagga
    20281 caaagacagg ctttgaccct agtcatggca gctctggtga aacccaaact tttgcttttg
    20341 gatgaacata ctgcggcact tgacccgaaa accagtcaaa tggtgatgga cttgacgcaa
    20401 aagattgtgg agcaccatca gtggacgact ttgatgatta cccatgatat gaatcatgcg
    20461 attgagtacg gcaatcgtct cattatgctc tatcaaggaa agatagtggt agatgtcaag
    20521 ggagaagaga aaaagcatct gacagttgaa gaccttatgc atctcttcca gaaaaatagt
    20581 ggccaaagtc tggtcagtga tgaattagtt ttgggataaa gaaaaaggct gagatctggt
    20641 gtctcagtct tttatttctt gcgttttgca aaatctacaa atctaaattt ctctaactta
    20701 tggcgacttt cggtaaactg aaattggcgt ccgtcttgga gatagacttt tgatttaatc
    20761 gaaacgacat aagggtcttt gccaatgtcc atgagaatct tgtctcggtc gcttgagtgg
    20821 tcaatggtga tttccttctg agcataatca ataaggagtt tgaggccatt ttctatataa
    20881 gaatagatag actgctcagc aatttggcga gtgagatttg gaattagttc catatccaga
    20941 tagtccgtat ccagaacgga taccagatca tccaccacac gctggcggac caccttccaa
    21001 accatccgaa actctgggaa accggttatc agtgaggatt ttttatcaat aataatcttg
    21061 tccagactga ccacgttggt tttagagcgc agtccaagtt ctttaactag ttcttggtag
    21121 ctggttaggt tggatacagg gaaattgacg gtttcttctt tgacgacttg agaaccttgc
    21181 cctcttatct ttttgatcaa tccttcctct tggaggagag acagggcctt tcggacggta
    21241 tcacgactca cttgatattg ctccataagg tcgtgctcgc taggaaggaa atctccgaca
    21301 gcgtaagtct cgttttgaat ggtttcttgg atttgcttaa ataattgttg gtatttcttc
    21361 attttgcctg cttaaatagg tttttagtct agtttaatga acacctgtat atattttacc
    21421 atacaagtgc tagaaaattt acaaaaaaac acccttttta taccaaacaa gttgtagaca
    21481 agtttggtat cgtttacaat atacttatca aatcaaactt gctttgacaa gtataaggag
    21541 aatcaaatgg gaaaatttga acaagaagcc aaagatctgc ttcaggcaat tggaggcaaa
    21601 gaaaatgtga ctgccgtaac tcactgtgcg acacggatgc gctttgtttt aagagatgat
    21661 aagaaggcta atgttaaagc tatcgagtca attccagctg ttaaaggaac ttttacaaat
    21721 gcaggtcaat ttcaggtaat cattggaaat gacgtgccca tcttttataa tgattttaca
    21781 gccgtttcag gtattgaggg tgtttccaaa gaagcagcca agtctgcagc taagagtaat
    21841 caaaacgtgg tccaaggtgt tatgaccact ctggcggaaa tctttactcc gattattcca
    21901 gccttgattg ttggtggatt gatcctcggt ttccgtaatg tcttggaagg tgtccattgg
    21961 tcgatgttgg atggcaagac catcacagaa tcctctcagt tttgggcagg ggttaatcac
    22021 ttcctctggt tgcctggtga agctatcttc cagttcttac cagtagggat tacttggtct
    22081 gtttctcgta agatgggaac cagccaaatt ttgggaattg ttctcggaat ctgtttggta
    22141 tcgcctcagt tgctcaatgc ctatgcggtt gcttcaacgc cagcagctga tatcgcagca
    22201 aactgggttt ggaattttgg ttattttact gttaatcgta ttggttacca agcccaagtt
    22261 atcccagcct tgcttgcagg tttgagtctg tcttatcttg aaattttctg gcgcaaacat
    22321 atcccagaag tcatttccat gatttttgta ccttttttgt cattgattcc agccttgatt
    22381 ttggctcata ctgttttggg accaatcggt tggacaattg gacaaggact ttcatcagtt
    22441 gtcttggcag gtttaactgg tccagttaaa tggctcttcg gtgcaatttt tggtgccctc
    22501 tacgctccat ttgtcatcac aggtctgcac cacatgacca atgccattga tacacaattg
    22561 attgcggatg ctggtggcac tgccctctgg cctatgattg ctctttctaa tattgctcaa
    22621 ggctcagccg tgtttgccta ttatttcatg catcgccatg atgagcgtga ggctcaggtt
    22681 tcacttcctg caaccatttc agcctatctc ggtgttacag aaccagctct ttttggggtt
    22741 aacgtaaaat atatttatcc atttgttgct gggatgactg gttcagccct tgcaggcatg
    22801 ttatccgtta cttttaatgt aactgcggct tctattggta tcggtggttt gccaggtatt
    22861 ctctctattc aacctcaata catgctgcca tttgcaggaa ctatgctagt tgcgattgtt
    22921 gttccaatgc tcttgacttt cttcttccgc aaggctggtc tctttacaaa aacagagggc
    22981 gatacgaact tgcaggcaga attcgttgct caagaagaag cagaatttgt gaaccatgaa
    23041 ccagtagaac ttacttcggt agaaattatc agcccactaa ctggccaagt gaaagaattg
    23101 agtcaagcga cggatcctat ttttgcatca ggtgtcatgg ggcaaggtct ggtcattgaa
    23161 ccaagccaag gtgagttgac ctctccagtt aatgggacag tgacggttct tttccctacc
    23221 aagcatgcca tcggcattgt ctctgacgag ggagttgaat tgctcatcca catcggtatg
    23281 gatacagtag gtcttgatgg caaaggtttt gaaagtcttg tagtccaagg agatcacgtt
    23341 acagttggtc agcaactgat tcgttttgat atggatgtca ttaaggctgc aggtctggtg
    23401 acagaaactc ctgttatcat caccaaccaa gatgcttata cagcgactat tcccggaact
    23461 tatccgacaa cgatccaagc tggagcatct ctcatggtcg ctacacgaat ctaatactct
    23521 tcgaaaatct cttcaaacca cgtcagctct atctgcaacc tcaaaatagt gttttgagca
    23581 acctgtggct agcttcctag tttgttcttt gattttcatt gagtataatt gaaatagcac
    23641 aaatagggac ggaagactgc ttcctccctt ttatcagaaa aggagaaaca gatgacactt
    23701 gataaaggaa aagtagtcta tcaaatctat ccaaaatctt acaaagacac cactgaaaac
    23761 ggtattgggg atttccgtgg gattatcgaa aaaattccct atttagccaa actaggtgtg
    23821 gatatggtct ggctcaatcc attctatcca agccctcaac gggataatgg ttacgatatt
    23881 tcagattata tggcagtgga tcctcttttt ggtgatatgg ctgattttga ggaaatggtg
    23941 tgtgtcggta aagagcacaa gattgacttt atgctggaca tggtacttaa tcattgttcg
    24001 acagagcatg aatggtttca gaaagcccta gctggtgata agtattatca agactttttc
    24061 ttcatccaag accaaccaac agattggcag tctaagtttg gtggttctgc atgggcgcct
    24121 ttcggggata ctgggaaata ctatcttcac ctatttgatg agacccaggc tgaccttaac
    24181 tggcgcaatt ccaatgtccg taaggagctt ttcaaggttg ttaatttctg gcgcgacaag
    24241 ggtgtcaaag gttttcgatt tgatgtgatc aatttgatcg gtaaggacga ggtttcagtg
    24301 gactgtcctg aaaatgaagg gaagccagct tatacagaca aacccatcgt tcataactat
    24361 ttgcgtatga tgaaccaagc cacttttgga tctgacgata gctttatgac agttggggaa
    24421 atgtcttcta ccaccatgga aaactgtgtc ctctattcgt cgcctgatcg tcaggaatta
    24481 tccatgacct ttaattttca tcacctcaag gtggactata aggatggaca aaagtggacc
    24541 ttggctccct ttgattttga agagttgaag agtctttacc atagttgggg taaggaaatg
    24601 agtgataaag acggttggag tgccctcttt tggaacaatc acgatcaacc acgtgccttg
    24661 aaccgttttg tcgatattca aaacttccgc aaggaaggag ccaccatgct agcagccagc
    24721 attcacctgt cacgtgggac accttatatc tacatgggtg aggaaatcgg gatgattgac
    24781 ccagactatg attctatggc tgattatgtg gatgtcgaat cgcttaatgc ctatcagatg
    24841 ctcttagaag aaggaaagag tcagcaagaa gccttccaga ttattcaagc taagtcgcgt
    24901 gataattcac gcattcccat gcagtgggat gcttcggaaa atgcaggatt ttcaacaggc
    24961 actccttggt tgaaagctgg taaatcctat aaatacatca atgtagaaaa tgaaatccag
    25021 ggtccgattt ttaccttcta tcaagacttg attcgactgc gtaaggaaat gccaatcatt
    25081 tcagaaggaa gttacaaacc agcctttgaa gatagcaaac aagtctacgc ttttgaacgc
    25141 cagtttgagg atcaaaagtt actagtgctc aataactttt atgctaaaga agtggaaatc
    25201 gacttgccag cagtctacca aaatggacag attttgatat caaactatga agatgcagaa
    25261 gtgtctgaga aaattctact caaaccctat caaacactgg ctatttatgt aaattaatac
    25321 tcttcgaaaa tctcttcaaa ccgtgtcagt tttatctgca acctcaaagc ggtgctttga
    25381 gcaacctgtg gctagcttcc tagtttgctc tttgattttc attgagtata aacttagcaa
    25441 ggagacctct ttttcagaaa agcagggttc tttttgctgt ctattccatc cctgaaagtg
    25501 tagaacaaac tggtcagggt ggctagtttg tggtataatg aaagggactc aaaaagaaac
    25561 aggagaaaaa tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga
    25621 ggagctcttg gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac
    25681 ccacgtttga atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc
    25741 gaagctaagg tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct
    25801 aacaataaaa agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac
    25861 tatgttggtt atggtttagg gatgagactt tttccttgca ttctattgat atttgattat
    25921 gattaagaga gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg
    25981 aatcataatg aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac
    26041 caattggttt tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc
    26101 agcttcccca tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc
    26161 gtcctgctga gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg
    26221 atgtcaaaat gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca
    26281 aggctcaact agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca
    26341 agtccagtca aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca
    26401 tataccgtca gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc
    26461 ccaagtttgc tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg
    26521 tctatgaaac cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc
    26581 attatccact ccttcgccct attatccaaa atgtgatggg gccaaaggtt cagctcatcg
    26641 atagtggggc agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc
    26701 gtggtcgcga tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt
    26761 ttgcacaaat tggtgaagaa tggctggaaa aagagattca tgtggagcat gtagaattat
    26821 gacaaataaa atttatgaat ataaggatga ccaggactgg tatgttgggt cttatagtat
    26881 ttttggtggc gttaacagtt tgagcgacta taagacagat tttcctctgt ttgaattctc
    26941 caaaatattt ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg
    27001 ttctacctac cgtttgttct cctttgtggt agacatgctt aatcaagaaa tgggacgaaa
    27061 cttggaagtt attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta
    27121 tgtagaattg cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag
    27181 agaaaccttg ttgattgcga ctcgtaacga aggtaaaacc aaggaattcc gagctatctt
    27241 tgataagtta ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc
    27301 agaaacaggt atgacctttg aagaaaatgc ccgccttaag gcagaaacca tttctcaatt
    27361 aacgggcaag atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt
    27421 accaggcgtc tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc
    27481 caaactcttg cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca
    27541 cacaacccta gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc
    27601 aggttatatt aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct
    27661 tgtaggagag acaggtgagt catcagctga attaaccctg gaagaaaaaa atagtcaatc
    27721 tcaccgtgcc ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc
    27781 atcattgtaa tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc
    27841 tatgtgggca aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct
    27901 ccactttggg agggcatccg cgttgttaaa gggaacatgg acttctacgc cggctaccca
    27961 gaacgtctgg tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt
    28021 gacatcaatt tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc
    28081 tgcctctatg gtcacttgca tgtgccaagt gcttggatgg aaggcaagat cctctttcta
    28141 aatccaggtt ctatcagcca accacgaggt accatcagag aatgtctcta tgctcgtgtg
    28201 gagattgatg atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca
    28261 ggtttgtcca aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc
    28321 aggaggaaac ttttttgacc cctgctaaaa atctagctgt gttgattgat acccacaatg
    28381 cggatcatgc gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag
    28441 atgaaaaaca gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc
    28501 atgacttgag ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg
    28561 tagcggttgt ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt
    28621 ccttcttacc ggttgtggat gcagagggta ttttccaagg gattattacg cgcaagtcca
    28681 tcctcaaggc cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc
    28741 aatgagagac aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa
    28801 gcagtcctat aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga
    28861 gaccagtctc aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg
    28921 aaagatttcg gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag
    28981 cttttaccgc ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct
    29041 atacctagac tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt
    29101 aatcctagaa atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa
    29161 tctggatttt caggtgttgc gaatcagcaa ggcttcccaa cggaggattg tcaccattcc
    29221 cacggccttg ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg
    29281 agggaaaccc tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa
    29341 aggttttcca tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga
    29401 aaacaaggtc gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt
    29461 agaaaaatat agataatgga tattaaatta aaagattttg aaggacccct ggacttactc
    29521 ttgcatctgg tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc
    29581 gaacagtatc tagcctatgt ctcaaccctg caggccatgc gtctggaagt gacgggtgag
    29641 tacatggtca tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta
    29701 gcagaagtga cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa
    29761 tatcgcaagt tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag
    29821 tattattcca aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag
    29881 acgaccattg acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt
    29941 gcacaaaatc acacgactat cttgcgggat gagtataaga ttgaggacat gatgattatc
    30001 gtgaaagaat ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc
    30061 cagaatgtcc aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag
    30121 gagctgatcc tcgtgcaaga ggagagtttc ggagatatct atctcatgga aaagaaggaa
    30181 gaaagtcaag tgcctcaaag ctcgacttga tagagaggaa agatgagtac tttagcaaaa
    30241 atagaagcgc tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa
    30301 ctcctctctc tgccaccgac aggcatccag caaagtttag gaaaattagc ccagaagtat
    30361 gaaaaggacc cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg
    30421 accaagcctc aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc
    30481 ttgtctcggg ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg
    30541 atagaaattg atgccatccg tggggttaac tcgagtggag ccttggcaaa gttgcaggct
    30601 tttgacctga taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg
    30661 actacggatt atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt
    30721 gatgaacttg agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat
    30781 gagaatcaat aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct
    30841 gattaagcaa ggcttggtga cggttaacgg ccaagtggtg cgtgaactag caaccactat
    30901 caagtcaggc gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggtcta
    30961 ctatctgctt aacaaaccac gcggtgtgat ttccagtgtg acagatgata agggtcgcaa
    31021 gacggttgtc gacctcttgc ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga
    31081 ctgggataca tcaggtgtct tgattttgac caatgatggg gactttacag acgagatgat
    31141 tcaccctcgt aatgagattg acaaggttta tgtcgcgcgt gttaaaggtg tggccaataa
    31201 ggacaatctc cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc
    31261 tgtttatgaa attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat
    31321 ccatgaaggg cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga
    31381 taagttgtct cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc
    31441 ccgtcgtctt aataaaaaag aaatcagcca actacacacc atggctgtaa ctaaaaaata
    31501 atgaaacgaa ttttaatagc gcctgtgcgc ttttaccaac gttttatctc accagtcttt
    31561 ccaccctctt gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa
    31621 catggtttta agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg
    31681 aaaacaggta aggaccccgt tccagaccac ttttccctta aacgaaatca agaaggggaa
    31741 tgaggtgggg taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtaaac
    31801 ttgataggat gcgttttaga atgtcaaaat tttatactct tcgaaaatct cttcaaaccg
    31861 cgtcagcttt catctgcaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta
    31921 gtttgctctt tgattttcat tgagtattaa attgagtttg aagtggctta tttcaaagct
    31981 ttttgtatgt cttcaatcat gagttttgtt gattcaagtc cgcctccgct tagataccag
    32041 aggtctggtg ttagttggat aatcttacca tttttagcag caggtgtttc agcgataagg
    32101 gcattttcta ggacaccgtc gttgctagag ttgtccccac cgatggcaag ggtacggttg
    32161 atgacaaaga ggatgtcagg gttgatttct ttgacacttt caaagctgac ttcttgtccg
    32221 tggcgtgagt cttcaaattt tgtatcagtt ggtttgaatt tcaaggtttg gtacaagaaa
    32281 gagaaacgag atttggcacc aaaggctgcc atttttcctt cattaaggag gatcgcaagg
    32341 gcttttttgt cagagctttc atttttagta gcgacttctt ggatgctctt gtctagcttg
    32401 gccaattctt ccttggcttt ctgtgtacca gtttcgccga aggcacttgc taaggattcg
    32461 atattagcct tggtagaagt ccagtagtcg tccttgcttg cttggaagag aacggttggg
    32521 gcgatttctt tgaatttgtc tacgaatttt tgtgtacgtg gtgaagcgat aatcaaatca
    32581 ggctcaaggg cggcgatagc ttctaaatca ggttctttca tagaaccaac atttttgaca
    32641 gttcccacta ggtcttttag ataagtcgga acagtttttg taggcattcc gacgatattt
    32701 ttttcaaatc ctaaagcgcg aatagtatcc gcagcgccga ggtcaaaggt cacaatcttt
    32761 tcaggaactt tggaaagttt gacctcggcc agtgaacttt taatggttac ctctgttgga
    32821 gcagagctac tggtctctgt ctgactagtg cttgagtttg tactacatgc accaagtagg
    32881 agcaagaagc tggccactag ggcagtgaaa taaagtttaa gggatgtttt cataatttct
    32941 cctctttaaa atgtgataac gatttaggga gtctcttgat cttattgact aagagactga
    33001 aggttctcta acttgagctt ttatgttact agctatagat acagatcttt ttgtcattga
    33061 tatcagctag cgtgatggga atctcataaa gttgactgag caggtccgcc tgcatgattt
    33121 gatcggttct tcccttgcta aagacctggc cgtccttgaa ggcgacaatt tcatctgcat
    33181 actgactggc tatgttgata tcgtggagga cgatgataat ggtcttgccg agttcctcca
    33241 ccagtcgtcg aagaatctgc atcatgctga cgctttgctt gatatcgaga ttgttgagtg
    33301 gttcgtccag caagataaag tccgtatcct gggccagtac catagcgata aagacgcgct
    33361 ggagttgccc ccctgacagg ctattgatgt agcggtcttt taagttggtc agttccaaat
    33421 agttcagagt ttctcggatt ttttcccagt cttctgatct aagtcgacct cggctgtagg
    33481 gaaaacgtcc aaaactgacc agttcttcaa cagtcaattt ggcttggtaa ttgattttct
    33541 gttttaggat ggttagttct tgggccagtt cttgcgaatt ccagctctcg atttcacgtc
    33601 ctttgatact gagaactccc tgatctttct tggttagtct gctcatgatg gagaggagag
    33661 tcgattttcc agcaccattt ggaccaataa aggctgtcag tttttgagga ctgacttcaa
    33721 gcgaaatgcc ttgcaaaata tcctgttttt gaatggattt gtcaatgttt tccagtttca
    33781 ctgacgagcc ctcctatata gtaagataaa gaataagaag ccacccacac tctcaatgat
    33841 catactgata cgaatttcca gtgcaaagac tcgttcaatc aaggcttgcc ccaaggttaa
    33901 gctaataaat ccaaccagaa tggccactat aaagagtaac ttgtgctgat agtctttgac
    33961 aatcaggtag gtgaggttgg ccagcataaa gccgaagaag gccataggtc ctaccaaggc
    34021 agtggccgtt gaggtcaaaa gcacgattcc ccagaggagc tctttctgtt ctttttcaac
    34081 atcgagtccc aatatctgag ccgtttctct ttgcaggtgc aagacatcta gaacgactgc
    34141 ttttcgaaag aaaaagattg tcaaagcgag gatgatcaga gaaccgatgg ctaggatgga
    34201 agtgttgaga tgttgaaagg aggcaaaaag actattttgc agtttatcgt attcgtttgg
    34261 atccattagg acttgaagga aggtgctgat atttcgaaag agacttctga gcgctagaca
    34321 gatcagcagg atgaagacca ggtcttgctt catcagtgtc ttcaagtaac cttgtaaggc
    34381 gagaaagaag agggactgga caagaagtaa gactaggaat tctaagatag gggatttgcc
    34441 aagttgaaga aacttgcttt caaaaaccag tagtagggtt tgtagtagga cgtagaagga
    34501 ttcaattccc aaaatactag gcgtcaggaa gcgattttcc gtcagggttt gaaaactaat
    34561 ggtcgaaatc ccagtcgcga tggctaccaa gagataaacg atgatctttt gggaacgcaa
    34621 cttccaagca aaggctgaca agtgagtgat gggccaaaag tagagaagac aagctccgat
    34681 ggtaagaata atgagaatcc agaagagctt ggtatgtttg cttttagtct gcatcttttc
    34741 gtccccctct ccagagaagt aggataaaga cgagactacc gatgattcct agcaagagac
    34801 tgacagacaa ctcatagggc ctaatcagaa ctcgggatag gatatcgcaa gccagaacta
    34861 gattggcacc aaccagtgcg accatgagtt tggtttgact tagattatct ccatagcgct
    34921 tgcgaacaag attgggaacg ataactccga gaaatggtaa gccacccacg gtaatcatgg
    34981 tgacgcttgt cgttagcgcc accagaaaga gggccagttt ttcaagtagg gagtaggaaa
    35041 tccccaaact ctcgctggtt tctttcccta gattcatgat ggtgaaggtt tgggataatt
    35101 tccaaacggt tatcaggatg atgaggccta agaagagcca ctcatactga tgggtctgaa
    35161 tcatggagaa ggagccctgg gtccaggcag tcatactctg aaccagattg aaacgatagg
    35221 cgataacttc tgtgactgag ccgataatcc cgctatagat gatcccaatc agaggcaaca
    35281 tccacctttc ctttacagta aaaatggtca taaaggctag gaagaagagg gtgaatacga
    35341 tggatgaaac aaaagcgaag agcatctttt gggtcagact agccgatgga aagacaaaaa
    35401 ggctcagcac cattcccagt ttggcggctt cagtcgttcc aactgtactc ggtgcagcaa
    35461 actgattttg ggtaatagtc tgcatgagaa ggcctgccat actcatacta gaggcagtca
    35521 ggagaatact gatagttctt gggagacggg actcttgaaa gaggagccag gtctgctggt
    35581 cgaaatcaaa tagctttccc catgaaaaat cactggtccc aatgctaata gagagaaaga
    35641 ctaggagtag aagtaagcca attaaataat gagaaagttt cataccccgt cctttcatgt
    35701 agatttggta tcgaaagata tctgcggata taaatgtaac attatttttc taatctgtca
    35761 ataaaatttc tgacaattta ataaatacaa caaggagaga gcaacaagac tttctccttt
    35821 gttatcctat tctaaaatgt ttttacctta atctgataaa ataatatctt cgagggagta
    35881 gctagccgtc caatcaagat attgtttagc ttttgaagca tctgctagga cactggctgg
    35941 gtcactagca cgtcgagcaa caatctcgtg tgggattttt taatttagta attcttcagc
    36001 agttttaaag atttctttga tagtatagcc ttttttagtt cctaagttaa agatttgaga
    36061 agaactgtct tcttgaaata ggtagttcat tcctttaaca tgagcctatg caaggtccaa
    36121 gacataaatg taatctcgaa tacatgaacc gtcacgtgta tcgtagtcat ctccaaatat
    36181 tttttaagct atcatgttgt cccaatgcgg tcttgttgat atttggaatg atgtgagttg
    36241 gatttttcac acgcagaccg tttgaagcat ccatttcagc cccagcaaca ttaaagtaac
    36301 ggaaaataac atatttccag tcgtagcgat tggccatcca gtaaatcatt cgttcgccca
    36361 tcagttttgt ctctgcataa gggttgacag ggtcgagcag ggtatcttca gtcaccggct
    36421 tgtcaataca gttatttcca tagagagaag cagtcgaaga gaacatgatt ttttgaatgc
    36481 caacttcaga taagactttg agaacttggt tcataccagc aacgttggca gtgaagtatt
    36541 tacttggatt ttcaatactt tcgctcacaa caatttcacc tgcacaatgg aggacagcat
    36601 caatctgatt ttcttctaag taagccttta aggcgctagc atcataaaca tccagttctt
    36661 taaagctagc acgactatcc acagccgctc gatttcctgt ggagagatta tcgaggacat
    36721 gcacctgata gccagcattt aaaagagctt taacggtatg ggagccaatg tagctagtcc
    36781 cacctgtaac aagaattgtt ttggtcatga ttttttcctt ttctagtttt gtactatagt
    36841 aaattgaaat aagatatgaa caact
//
LOCUS       DAVKZB010000008        35476 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKZB010000008 DAVKZB010000000
VERSION     DAVKZB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.008"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(129..1763)
                     /locus_tag="VXA80_000372"
     CDS             complement(129..1763)
                     /locus_tag="VXA80_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352919.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1744..2493)
                     /locus_tag="VXA80_000373"
     CDS             complement(1744..2493)
                     /locus_tag="VXA80_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352920.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2672..4126
                     /locus_tag="VXA80_000374"
     CDS             2672..4126
                     /locus_tag="VXA80_000374"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV0352921.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKTQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVERTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYNW
                     ADWFMETTDVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4216..6834)
                     /gene="alaS"
                     /locus_tag="VXA80_000375"
     CDS             complement(4216..6834)
                     /gene="alaS"
                     /locus_tag="VXA80_000375"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV0352922.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     KYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEADMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6856..7341)
                     /locus_tag="VXA80_000376"
     CDS             complement(6856..7341)
                     /locus_tag="VXA80_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV0352923.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPHFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7616..7855)
                     /locus_tag="VXA80_000377"
     CDS             complement(7616..7855)
                     /locus_tag="VXA80_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352924.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8116..9186)
                     /locus_tag="VXA80_000378"
     CDS             complement(8116..9186)
                     /locus_tag="VXA80_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0352925.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIIYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9183..9956)
                     /locus_tag="VXA80_000379"
     CDS             complement(9183..9956)
                     /locus_tag="VXA80_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352926.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGNDMNSFIGFSLSH
                     FKTMFGDGRLMLIVTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9953..10759)
                     /locus_tag="VXA80_000380"
     CDS             complement(9953..10759)
                     /locus_tag="VXA80_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0352927.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10740..11897)
                     /locus_tag="VXA80_000381"
     CDS             complement(10740..11897)
                     /locus_tag="VXA80_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0352928.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11956..12906)
                     /gene="murB"
                     /locus_tag="VXA80_000382"
     CDS             complement(11956..12906)
                     /gene="murB"
                     /locus_tag="VXA80_000382"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV0352929.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13018..13770)
                     /locus_tag="VXA80_000383"
     CDS             complement(13018..13770)
                     /locus_tag="VXA80_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV0352930.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAPYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13780..14511)
                     /gene="budA"
                     /locus_tag="VXA80_000384"
     CDS             complement(13780..14511)
                     /gene="budA"
                     /locus_tag="VXA80_000384"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV0352931.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGGFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14653..15924
                     /locus_tag="VXA80_000385"
     CDS             14653..15924
                     /locus_tag="VXA80_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV0352932.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15944..16759)
                     /locus_tag="VXA80_000386"
     CDS             complement(15944..16759)
                     /locus_tag="VXA80_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0352933.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16916..17569)
                     /gene="phoU"
                     /locus_tag="VXA80_000387"
     CDS             complement(16916..17569)
                     /gene="phoU"
                     /locus_tag="VXA80_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0352934.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17581..18339)
                     /gene="pstB"
                     /locus_tag="VXA80_000388"
     CDS             complement(17581..18339)
                     /gene="pstB"
                     /locus_tag="VXA80_000388"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0352935.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18352..19155)
                     /gene="pstB"
                     /locus_tag="VXA80_000389"
     CDS             complement(18352..19155)
                     /gene="pstB"
                     /locus_tag="VXA80_000389"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0352936.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19166..20050)
                     /gene="pstA"
                     /locus_tag="VXA80_000390"
     CDS             complement(19166..20050)
                     /gene="pstA"
                     /locus_tag="VXA80_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0352937.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20040..20957)
                     /gene="pstC"
                     /locus_tag="VXA80_000391"
     CDS             complement(20040..20957)
                     /gene="pstC"
                     /locus_tag="VXA80_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0352938.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20957..21835)
                     /gene="pstS"
                     /locus_tag="VXA80_000392"
     CDS             complement(20957..21835)
                     /gene="pstS"
                     /locus_tag="VXA80_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV0352939.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22409..23713)
                     /locus_tag="VXA80_000393"
     CDS             complement(22409..23713)
                     /locus_tag="VXA80_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV0352940.1"
                     /translation="MQFPEGFVEKYEEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESANRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLFPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23714..24487)
                     /locus_tag="VXA80_000394"
     CDS             complement(23714..24487)
                     /locus_tag="VXA80_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV0352941.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCRNDKLLAPFQAKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYVTYLQPWDYAAASILGESLGY
                     RVVTLSGEAPDFQTRQPVMMLPLEMQEEIQSYIYERKRT"
     gene            complement(24477..24755)
                     /locus_tag="VXA80_000395"
     CDS             complement(24477..24755)
                     /locus_tag="VXA80_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV0352942.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24755..25156)
                     /locus_tag="VXA80_000396"
     CDS             complement(24755..25156)
                     /locus_tag="VXA80_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV0352943.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25366..25983)
                     /locus_tag="VXA80_000397"
     CDS             complement(25366..25983)
                     /locus_tag="VXA80_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV0352944.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(25973..26746)
                     /locus_tag="VXA80_000398"
     CDS             complement(25973..26746)
                     /locus_tag="VXA80_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV0352945.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     DIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26757..27494)
                     /locus_tag="VXA80_000399"
     CDS             complement(26757..27494)
                     /locus_tag="VXA80_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV0352946.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIALGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27499..28629)
                     /locus_tag="VXA80_000400"
     CDS             complement(27499..28629)
                     /locus_tag="VXA80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV0352947.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28713..29102)
                     /locus_tag="VXA80_000401"
     CDS             complement(28713..29102)
                     /locus_tag="VXA80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0352948.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQIESGEITVDSILAQVKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29118..29501)
                     /locus_tag="VXA80_000402"
     CDS             complement(29118..29501)
                     /locus_tag="VXA80_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV0352949.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29502..30290)
                     /locus_tag="VXA80_000403"
     CDS             complement(29502..30290)
                     /locus_tag="VXA80_000403"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV0352950.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30283..31218)
                     /locus_tag="VXA80_000404"
     CDS             complement(30283..31218)
                     /locus_tag="VXA80_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV0352951.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31348..31524)
                     /locus_tag="VXA80_000405"
     CDS             complement(31348..31524)
                     /locus_tag="VXA80_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV0352952.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31664..32371)
                     /locus_tag="VXA80_000406"
     CDS             complement(31664..32371)
                     /locus_tag="VXA80_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV0352953.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32523..33551
                     /gene="queA"
                     /locus_tag="VXA80_000407"
     CDS             32523..33551
                     /gene="queA"
                     /locus_tag="VXA80_000407"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV0352954.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLESGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34212..34601)
                     /locus_tag="VXA80_000408"
     CDS             complement(34212..34601)
                     /locus_tag="VXA80_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0352955.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34655..35347)
                     /locus_tag="VXA80_000409"
     CDS             complement(34655..35347)
                     /locus_tag="VXA80_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0352956.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSML"
BASE COUNT        10550 a         8091 c         6745 g        10090 t
ORIGIN      
        1 ttatatttct tattcaaaac attcctccct atatttttga ttaccaatct taatcattta
       61 caactacatt ctaacaaact ataaaagcgt ttgtcgaatt gaatttatca agcaagcgac
      121 caaccagctc atcttttttc tatttctgcc aatatgcgtg acaggtaata atgatagcca
      181 aaaatagcaa gagcaagcag gaggataaga gctcctactc ccaagctgat ggcaaggata
      241 gtggagagag actgaaccaa gaatatgctc ccaattacaa gggccatcag gattgcacta
      301 taaataaaca ataaaactat ggcgactatg ccatttgaac gattcaccag gtccgtaatg
      361 ctactccaat tggttgacag atttttaacg tccttaaagt aatggtggca agaaaggatg
      421 acactggcaa ggatccatgc cacaataagg taaatcatcg aaatgatggg caagcctaga
      481 tatagagaaa gaccaagtaa agtcagaatt ggtaaaaagg actggacggc atatataatc
      541 caaaatttca ctttcacata acgagcaaag tcaaagggta aactcttaag aaaatcaaca
      601 ttttccctct ccaaggacaa ggcaattgaa tgcaggctgg tgatattgtt attgacaact
      661 gctataaaga gagctataaa aaacaagggt aaccagtatg gaggatgaat gtctggaact
      721 atctgagaat ctcggatttt ggaaatcaga ccgatcatca tgagataagg aaggaaagca
      781 cttgtaaaaa gcactgtaac cacgccagtc ccctgtccca agagggtgag gtggtagcgt
      841 aaaaccatgc ggaaaaatcc ctttttagtg gttgaaattc tctccttgct gcgacgttct
      901 tttttgacct tatcctcact attaagcagg atcacgtcat aaaaacgagg aaggaccttc
      961 tttttggtca gataaagcag gaagagagtt agtcctatcc aagcgagcag acccactaag
     1021 gcttctgtcg aaaaaggctc cactgctatt ttgtaaaaga tatgaagagg ataaaggaga
     1081 aatggaatgt ctctaacttt gtcaacaata cttccaaaag tcgactgaag aaagaagata
     1141 aatattaaag gtatgagaac tcctatccca atcatcacat tcgaaaaaat agactgatac
     1201 tttctgaaga ccctagtttg agccaagaaa tgcactgcca ctaccatcac tagagccaca
     1261 gagacaaata ataaggtcaa ggacagtagc atcaaaggca aacccagcca tagagaagga
     1321 gctagcctaa tgtagaggac cagaaaataa gctaggattg gtacaattcc agttagagct
     1381 ggcaaaagga cagacagtcc tttagcaatt ataatctctg attctttaaa ggcatagggc
     1441 ctatacgata tcaaatcctt actctcataa aagacattgt aaaaggccgt taaagaagtt
     1501 gaaaaggcaa tcactagtaa aatagcaatc atcgagctaa aataaatagg tatttcctca
     1561 aaaggaaaat gaatggctat attactaaaa cagatgagca tcaagagact ggaaaaaatg
     1621 taagaactta ggactctagc ggaaacattt acttttttcc caggattctt agcctgcttc
     1681 tttcgtagat tagccagatt agcttcttga gatgaataga ggatattgat atcaactaat
     1741 tttttaatga ccttgagacg catctgaaac ctcctctttt ctaccagcaa ggctaaggta
     1801 gatactttcc aaagactggt ctgggtggtc tttcctcaag ttctctacac taccacaata
     1861 gatcaaatgc ccctttttca aaatagcaat ccgatcacag acttgctcgg ctacctctag
     1921 gacatgagtt gaaaacaaga ccgtcttgcc tttttgtgca tgttccttca tcatttgctt
     1981 caaatcaaag gcggcctggg gatccaaacc agtcaagggt tcgtccaaaa cccaaatatc
     2041 aggagcagac aagagtgccc caatgacaaa gactttctga cgcattccgt gagaaagagt
     2101 ttcaataacc tgatagcggt tttcagcaaa atcaaaaacg ttcaatagcc tagctagact
     2161 agtctccaag tcagagctag tcagatcata ggatgaagcg atcaattccc aaaattcatt
     2221 ggccgttaag cgtaaaaata agtcaggcga gtctgctacg tagccaatct ttcgtttgat
     2281 cgccaagcga ttttccgata actcctgacc gtctaccaaa atacgaccac tgctgggtga
     2341 aatgatactg actagggatt ttatggtggt cgattttcca gccccattat gaccaatcaa
     2401 gcccataatc tctccatttt caatctgcaa attgagatta ctcaaggcct ctttatcacc
     2461 atacaactta ctaacatttt gaaattcaat catgggacaa ctcctatctt tatctgtatt
     2521 atacatcgct atactagcac tttgatacaa aaaaatcaat actttatttt aagtagttaa
     2581 aatagtttcc ttctatctta cgcaaacgtt tgcgctaata gatgctttat gatagaataa
     2641 agaacaagat tgacaagtaa gaggaaacat catgcaaaat caaacactca tgcaatactt
     2701 tgaatggtat ctgccccacg acggtcaaca ctggacgcgt ctggctgaaa atgctccaca
     2761 cctagctcat ctggggatca gtcacgtctg gatgccacca gccttcaagg caaccaacga
     2821 aaaagatgtc ggctatgggg tctatgactt atttgactta ggagagttca accaaaaagg
     2881 gactgtccgc accaagtatg gtttcaaaga agactatctt caagccattc aagcccttaa
     2941 aacacaggga attcaaccta tggccgatgt agttctcaac cacaaggctg ctgctgatca
     3001 cagggaagcc tttcaggtta ttgaagtgga tcctgtagag cgtacagttg aacttggaga
     3061 acccttcacc atcaatggct ggactagttt taccttcgat ggtcgccaag atacctacaa
     3121 tgacttccac tggcactggt accacttcac aggtacagac tatgatgcca aacgccgtaa
     3181 gtctgggatt tatctgatcc aaggggacaa caagggctgg gctaacgagg aattggtcga
     3241 taacgaaaac ggaaactacg actacctcat gtatgccgac ctagacttta aacatcctga
     3301 agtcatccaa aacatctata actgggctga ttggttcatg gaaacgactg atgtagctgg
     3361 tttccgtttg gatgccgtta agcatattga ctctttcttt atgcgcaact tcatccgcga
     3421 tatgaaggaa aaatatggtg acgatttcta tgtttttggt gaattttgga acccagacaa
     3481 ggaagccaat ctggactatc tcgaaaaaac ggaagaacac tttgaccttg tcgatgttcg
     3541 tctccaccag aatctctttg aagccagtca agctggcgca aactatgacc ttcgtggcat
     3601 tttcacagat agcctggttg aactcaagcc tgacaaggct gtgacttttg tcgacaacca
     3661 cgatacccaa cgaggacaag cccttgagtc taccgttgaa gaatggttca agccagcagc
     3721 ctatgccctc attttgttac gccaagacgg ccttccatgt gtcttttacg gagactacta
     3781 tgggatttca gggcagtatg ctcaacaaga tttcaaagaa atccttgacc gcctcctagc
     3841 catccgaaaa gatttggcct atggagaaca aaatgactac tttgaccatg ctaactgtat
     3901 cggttgggta cgttcaggtg ctgaaaatca atccccaatc gcagtcctta tctcaaatga
     3961 ccaagaaaac agcaagtcaa tgtttgtcgg tcaagaatgg actaatcaaa cctttgtaga
     4021 tttacttggt aaccaccaag gtcaagttac aattgatgag gaaggttatg gacaattccc
     4081 tgtctcagct agatccgtaa gtgtctgggc agtcaatacc atctaatagc tcataataac
     4141 caagctaggt ccaagcggat ttggcttttt tgtattcaca aaaagaccta cccaaatgga
     4201 tagatcttta cttgattaca atttacctgc tactgcatcc aacaattctt ggatcttagc
     4261 ttggttgctt cctcctgcca tggccatgtc tggtttacca ccaccacgtc catcgatgat
     4321 tggtgctaat tctttgacaa ggtttcctgc atgaaggtct tttgtcttgc ttgctacaag
     4381 gacattgact ttgtcaccga tagcggcaac taggacaaga agatcagagt agtctttttg
     4441 tttccagtta tctgcaaaag tacgaagggc accggcatcg gatacagaca cttgactagc
     4501 aatgtaacga tgaccgttga cttccttaac atctttgaag atatcgcctg cggctgcagc
     4561 tgcggctttt tctttcaact cagcattttc tttttgaagt tgacgaagtt gttcttgaag
     4621 tccttctacc ttgtgaggta cttccttgac ttgaggtgct ttcaaggttg ctgcgacagc
     4681 tttaagagca tcctcttgtt cacgataggc ttcaaaggct tccttaccag tcactgccaa
     4741 gatacggcga gttcctgaac cgattccttc ttctttgaca attttgaaga gaccaatctc
     4801 agaagtgttg ccaacatgag taccaccaca aagttcaata gagtagtcac cgatagtcac
     4861 gacacgaact tccttgccgt atttctcacc aaagagggcc atagctccca tttctttagc
     4921 agtgtcaata tccgtttcaa ctgtcttcac ttcaagtgct tcccaaattt tctcgttaac
     4981 ttgctgttca atcgcacgaa gttcctcagc agttactgct tggaagtggg taaagtcaaa
     5041 gcgaaggaat tcaacttcgt taagagatcc tgcctgtgtt gcgtggtttc caaggatatt
     5101 gtgaagggca gcgtgaagca aatgagtcgc agtgtggttt ttcatgacac ggtgacggcg
     5161 attgctatca attgccaagg tatattcttg gttcaaggca agcggtgcaa ggacttcaac
     5221 tgtatgaagg gcttgaccat ttggggcttt ctgaacattg gtcacagtag ccacaacctt
     5281 acctgactca tccaagattt gtccgtagtc agctacctgt ccacccattt cagcataaaa
     5341 tgacgtttcc gcaaagataa gagaggcagt tccttctgaa acagctccta cttctgcatt
     5401 gtcagcaacg atagctacca atttagaaga caattggcta gcattgtagt tgaagacact
     5461 ttctacagtg atgttttgaa gagtttcatt ttgcataccc attgagccac ccttgacagc
     5521 tgacgcacgc gcgcgttctt gctgttcttt catggctgct tcaaaacctt cacggtctac
     5581 agtcatatca gcctcttcag caatttcttc agtcaattca actgggaatc cgtatgtatc
     5641 atagagttta aagacatctt gaccagcaat gacagattga cctttttctt tcaagtctgc
     5701 cacaatgcct tgggcaaagt gttgacctga gtgaagggta cgggcaaatg attcttcttc
     5761 gctcttaacg attttctcaa taaagtcacg tttctcaagc acttctgggt agtagctttc
     5821 catgattttt ccaacagttg gaacaagttt gtaaaggaaa ggctcgttga tacccaattt
     5881 ttgaccatgc atagaagcac gacggagaag acgacgaaga acataaccac gaccttcatt
     5941 tcctggaagg gcaccatcac cgatagcaaa tgaaagagaa cgaatgtggt ctgcgataac
     6001 cttgaagctc atgttgtcgc catcttggtc ataaacctta ccagacaatt tctcgacttc
     6061 acggataatc ggcataaaga ggtccgtttc aaagttggtc ttagcccctt ggataacagc
     6121 caccaaacgc tccaaaccag cgcccgtatc aatgttctta tgtggcaatt ccttgtattt
     6181 gctacgagga acagcagggt ctgcgttaaa ttgtgacaaa acgatgttcc agatttcaat
     6241 ataacggtcg ttttcaatat cttctgcaag caggcgaaga ccgatatttt ctgggtcaaa
     6301 ggcttcccca cggtcaaaga agatttctgt atctggtcca gaaggtcccg caccgatttc
     6361 ccagaagttg tcctcaattg gaatcaagtg acttggatcc actcccactt caatccagcg
     6421 gttgtaagaa tctttatcgt ctggatagta ggtcatgtaa agtttttcag cagggaaatc
     6481 aaaccattca gggcttgtca aaagctcata agcccaagtg atagcttcgt cacggaagta
     6541 atccccgata gagaagttcc ccagcatttc aaacatggta tggtgacgcg cagtcttccc
     6601 tacgttttcg atgtcgttgg tacggatagc cttttgggca ttggtaatac gtggattttc
     6661 agggataatg gtcccgtcaa agtatttctt aagggttgct accccagagt tgatccacaa
     6721 aagagttggg tcatttacag gaaccaaact tactgatggt tctactgagt gacctttggt
     6781 cgcccagaaa tcaagccaca tttggcgtac ttgtgcacta gatagttgtt tcatattgtc
     6841 tccttattca cttgtttaat gtgattggct ttccagcatt tccacatagt caatcgcgac
     6901 acagagggaa atgactaggt ctgcataagc gtcttcaaga accgttacgg tataggtaga
     6961 agtcagatgg aagagttcct tcttaatttc cgcaatcaac tgatcgcgat catccagcaa
     7021 tttgaaattc aaatcccaga tattgccctc gatacgaaga cctagattat caaactcata
     7081 cttatctcgc cagaaggtca acttcttacg aatgacaaaa ctcgagccat cccgaagctg
     7141 aatctcaaaa tgaggaagta aggtcaagat ttctttacta atctcactga cttgttcacc
     7201 agccgcatca tagatggtaa aggttttagg aatcttaaaa aatgatccct ccacctgata
     7261 ggcaatttct cccctgtcat ccttgatagc gaagcgttcg cctccaagac gaaacttttg
     7321 tttgacaaga aatgttttca tcaacacctc caaaaatcaa aagacaagct catatcacga
     7381 agggcgaaaa accgcggtac caccttcatt caatgaactt gtcattctct tgttcttatg
     7441 caattgtatg attgagtagc atgacttcct agcttagatg gctcgcagca ccgccatttc
     7501 tctggactaa gacaagtgaa aatcaattct caactttctt attataacgt ttttttaagc
     7561 ttgcgtcaac tggaaatgat ctccgttgaa ttagaccaat tccctacatc tctgattact
     7621 ttttcaggat atattttttc ttactgccat ttttcttttt atcccaaatt ttcatattac
     7681 taaacacagc tactagaata tttccaaata taaaggtgcc tatcacccaa tatatggact
     7741 cagttgttag gtattgtcga tccaagccat cctttaaatg gaatagtata gcagtttggt
     7801 taacaatcat aaaggttggc cagaaacttt ttttgaaaaa agtagacatt ttcattattt
     7861 gttgccgctt tctgtaaggt taatactcaa taaaaatcaa aaagcaaact aggaagctag
     7921 cctcaagctg tacttgagta cggcaaggca acgctgacgt ggtttgaaga gtataggctt
     7981 agtatactac taggcaagca aataagcaaa taaacaaata aacaactaga atagaaaaag
     8041 atagggctct aaaaactgac ttctattcct taaaaacgaa ccagcttgac tgattcgtct
     8101 tcttacgttt atctcctact tccgatacat tttaaactgt aggaagaggt cgctatattt
     8161 ccctgtccat ttatggtcaa atttctcata aatttctagg tgtttcatgg tttcaacatc
     8221 gggatagaag gccttatctt cctttgtttc ctctgggagc aattccttcg ctggtaggtt
     8281 tggtgttgaa tagccgacat actccgcatt ttggagagca ttttcaggtt tcaacataaa
     8341 gttgataaag gcataggctg agttttggtt tttaactgtt ttgggaatga ccatattgtc
     8401 aaaccaaaga ttgctggcct ctgtcggtac cacataacgt agattttcat ttttttctaa
     8461 catttggctg gcttcaccag agaaggtcac gccgattgca acattattct gaatcatata
     8521 gcccttcatc tcgtccgcaa cgatagcctt gatatttgga gtcagtttgt agagcttatc
     8581 cactgtctct tccaactgct gcagatcctt ggagttgagg ctgtagccga gggaattgag
     8641 tcctagtccc agcacctcac gcgccccatc aaagagcatg atagaattct tatactccgg
     8701 cttccaaagg tcatcccaat gctcaggcgc ttcatctacc atggtttcgt tgtagataat
     8761 tcctaaggtt ccccagaagt aagggatgga gaatttatta cctgggtcaa aggactggtt
     8821 gagaaactct ggtccgatat tttcgattcc ttcaattttt gaataatcaa gcggaaccaa
     8881 gaggtcttcg tccttcatct tgttaatcat gtattcactt ggaatggcaa tatcgtaggt
     8941 cgttccaccc tgctttatct tagtgtacat ggcttcgttg gagtcaaaag tctcgtactg
     9001 aacttgaatt cctgtttctt ctgtaaactg agtcaagagt tcaggatcga tatagtctcc
     9061 ccagttatag ataaccaatt tttgactatc tcgactattg attttactat ctaaatgagt
     9121 cgcaattccc cacaagacaa ggataatcgc tgcaattcct gctaaaaatg aatagatttt
     9181 tttcatgctt gctcctcctt ctcacgagag ataaagtaat aacctacaac taggataata
     9241 ctaaagagaa agactagagc agacagggca ttgatttcta aggaaatccc cttgcgagca
     9301 cgagagtaaa tctcgactga tagggttgaa aagccatttc ctgttacaaa gaaggtcacg
     9361 gcaaagtcat ctaacgaata ggtgaaggcc atgaaataac cagtaatgat agacggagtc
     9421 aggtaaggaa gcatgatttc cttgaacatc tgaaattgac tagctcccaa gtcataggcc
     9481 gcatgaatca tgtcgccatt catttccttg agtcgaggca agaccatcaa gaccacgata
     9541 ggaatggaga aggccacgtg actagataga acggtcaaaa agccaagtga aaacttgagt
     9601 tgggtaaaga gaatcaagaa gctagcacca atcataacgt caggcgcaac catgaggata
     9661 ttattgagtg atagaaaggc ttcttggtat ttcttacgag actggtagat gtaaatggca
     9721 ccaaaagtcc cgataatggt cgctatcaag gctgatagga aggccaagaa aaaggtctga
     9781 gtcacaatta acataaggcg accatcacca aacatggttt taaaatggct caagctaaag
     9841 cctataaagc tgttcatgtc gtttcctgca ttaaaggcgt agccaatcaa gtaaaagata
     9901 ggcaggtaga ggaccagaaa gaccagtccc agataaaggt tggcaaattt tttcatcgtt
     9961 ctctcctttc cttagtcacc cacatggtga tgaacatggt caggatgaga atcacaccga
    10021 tggttgaacc cataccatag ttgtcattgg ttagaaaatt ctgctcaata gccgtcccca
    10081 aggtgataac gcggttccca ccaatcaaac gggtcagcat gaagagactc aaacttggga
    10141 taaagaccga ctgaacccca cttcgcacac cgttcataga tagagggaag atgacatgac
    10201 ggaaggtctc ccacttagtt gcaccaaggt cataactagc attgatgaga ttattatcca
    10261 tatcgtctaa gacattgaaa atcggcaaaa tcataaaggg gagctcgatg tagcttgcga
    10321 caaagataaa ggaaaaatcg gtaaaaagca actgttgtga accaattccg ataaattcca
    10381 agaattggtt aatagagcca ttttgaccaa aaatcccgat aaaagcatag gctttaagga
    10441 gcaaattgat ccaggtagga aggataatca gcatgagcca gagttgacgg tgcttgagac
    10501 gggtcaaaaa gagggctgtt ggatagctga taagcaaggt cacaaaggtc acaatccctg
    10561 cataaagcac tgagttgaaa ctcattttga ggtaggtcaa gttttgtgac gcaaagtaag
    10621 atttataatt ttctaaacta aactggcctt caatgttgaa aaaggattgt ccgaaaatca
    10681 agaccaaggg tgccaataca aagagggcaa tccaaagcat gtagggcact acaaagagtt
    10741 tagagcttgt tttcttcatc tctttcctcc tcgatcgcat tgatcagacc tgcttcttgc
    10801 tcttcgattt ccacgtattc ttcgatacga gcatcgaact cttcttcggt ttcattgaga
    10861 cgcatgatgt ggatgtcttc tgattcaaag tccagaccga tttcctcacc cacgatggcc
    10921 ttacgggttg agtggatcat ccattcattt ccaagttcgt cataggcgat aatttcataa
    10981 tgaactccac ggaagagctg ggtgtcgacc ttaacttgga gtttgccttc ttcaggaagg
    11041 gtaatgcgca agtcctctgg acgaataacg acctcaacag gttcatttgg cttcatccca
    11101 ccatcaaccg cttcaaagcg tttgccgtta aattcgacca agtagtcctc aatcatggta
    11161 cctggcaaga tgtttgactc cccgataaag gtggcaacaa agtggttgat tggctcatcg
    11221 tagatgtcca caggggttcc agactggaca atctcgccat cattcataac gaaaatccag
    11281 tcactcatgg caagagcttc ttcctgatcg tgagtgacaa agacaaaggt aatgcccaat
    11341 cgttgttgta attcacgcaa ttcgtactgc atgtctgttc tcaatttcaa gtccagcgct
    11401 gataaaggct cgtccaacaa gaccacacgg ggttggttga tgatagcacg ggcgatggcc
    11461 acacgctgac gttgtcctcc agaaagtttg cggatggaac gtttttcata accttccaac
    11521 tgaaccatct tgagaacttc cgctacacgc tgctcaattt ctttcttatc aattttacgc
    11581 aagcgaagtg gaaaggcaac attttcaaac acattcatat gtgggaacaa ggcataggat
    11641 tggaagacgg tatgtacgtc gcgcttgttg gttggaatat cattgatacg aacaccgtct
    11701 agcatgatat ctcctgtcgt cgcatccagt aaacctgcaa taatgtttag gatagttgat
    11761 ttccccgaac cagatgcacc tagaagggtg tagaatttcc cttcttccaa ctcaaagttg
    11821 atgtctttga gaaccttggt gttgctgtct tcaaaaactt tagagacgtt tttgaattcg
    11881 ataattggtt ttttcaattg gcataaattc cttctttttc atagattaac cgatcggggc
    11941 tctgtcaggt ccccactacc tcttgcaggg agtaaaacca cctgcataca tcttcgctac
    12001 cgataggctt tcacccaaga tccggacttc tctttcaagc gtaatacctg agtgttcctt
    12061 gactttttcg ataaccgatt ggatcaagtc ctcgtagtct ttggccgttc catctgcgac
    12121 attgatcata aatcctgcat gcttttctga cacttctacg ccaccgatac gatagccttt
    12181 caagccagct tctgaaatta actgacctgc aaaatgcccg actggacgct taaagaccga
    12241 gccacaagat gggtattcca aaggttgctt gagttcacgt aggtgcgtca agcggtccat
    12301 ttcctgcttg ataacctgat gggttcctgg agctagggca aatttaactg acaagacaac
    12361 tgcaccagac tcctgaatag ctgaatgacg gtaaccaaaa gccaagtctt tagcagacag
    12421 ggtttcgatt tctccatcct tggtcaagac cttacaagac tgcaagatgt gagcaatctc
    12481 gccaccatag gcacccgcat tcataaagac agcaccgcca acgcttcctg gaataccaca
    12541 agcaaactca aagccagtta aactatgacg gagggcaatg cgagttgttt caatcaagtt
    12601 agccccagct tctgcttcaa tggtatagcc atcaacagaa acgttattga gcttgtcaca
    12661 caagatgaca aatccacgaa tcccaccatc acgaacgatg atattgcttg cattgccaag
    12721 aaccatccaa gggatatttt cttggttggc aaatttcaca acgcgagcca actcaaaacg
    12781 atttcgtgga aagaccaaat aatcagcctc tccacctact tttgtataac tatagctatg
    12841 caagggttcc ttaaaacgga tatcaattcc ttctaagatt tcaagcattt tttctcttac
    12901 agacatgtca ctcttccttt tacaaaattc attccattat accattttta gagacatttg
    12961 acgaccataa aaataccttg tttggatttt gcataagaaa aagaggttcc ccccttttta
    13021 tgatttttta caaaagattt ccttggttcc ataggcgacc agaacgagct ccagtgctag
    13081 aatcacttca accaagactg gatttgtcaa ccagcctact tggaaaagag atggtgccag
    13141 atcaaagaag gcatgcaagc catagggtgc taggagataa atccatttct tctggcgaac
    13201 agcttggtaa acccaaactg tcaaaagtaa ttggaaacca agcgccaaga ttcgctcaaa
    13261 accaagcaaa taaatctgcc agaccgaaag tgactgaatg gtttttaaca tattttcaga
    13321 cagtaattgc ataacctgtg gattctgagt ttgaactgcc gaaagaacga tgtaaagatt
    13381 gagtaaacta gtaaggccta gaaaaatcaa ctccaagcca ccatgcccta ggccataggc
    13441 caaggcatct gccttttcca aacttctctt tttctccaac catttgaaga aaacaagacg
    13501 agccgtttcc tcaaaaaatg ctgccatggc taggccatag ataatataga taagcggatg
    13561 atcttgcaag agggcaatac taccgtcttt ttgagggtgc aaaatcaaga tatgcaccag
    13621 tttttccaaa atctgcgaag agacaaagaa agcgactgca cccaagccca agacagctag
    13681 attaatctgg tatttctttt tggcaaacca aatgcttcct atcagaaaag ctagcaacag
    13741 caacatggta ataatgatat gaatggtcat tttcttctcc tattctgcct tttcaatatc
    13801 ttttttcatc tcgtcaacat tgaacttagc aaacaagtat tgacggtctt ggactgggaa
    13861 acgttggtcc aactggtcaa ctgctcccac ctcgataatg ccttccttga tgacaaaatc
    13921 catgacatgt ccaccgaagg tcaaatcatc tgatatgaag tgcagatggt agcctgccac
    13981 actgacccca tggaaaatct caggcgtcca gaaaccaaca atagtccccg caacattgtc
    14041 acgactatat tccggttgat gggttgcgac atcagcaaac ttggtatcgg gtgttgactt
    14101 aggaatcata cgcacatgca tatgtgaaaa tcccccccga atcttgatag agcggaaaag
    14161 attttcccca tcataataag actcaattcg ttctttcaat tccttgtctg tcatctcaaa
    14221 gcgctggcgg aaaatgacct ctgcctgatg cggtaccact gcagcgtaag gaataagggc
    14281 atctggtgac acttccacaa tttctggctg gtctcctgac cctttggcct gataagcctt
    14341 gccatccaag acaatcaatt ccccatcaat ggaatccaag gttcccaaac caaggtcacc
    14401 atgttctagc aattctccta ctgtcatagt accaccataa aggccagcca tcaaggctcc
    14461 aagagtattg tattgaaata atttcactgg ttcctgcacg ttcttatcca ttcactttct
    14521 tgtctgttat tctataaatc ttactcctaa gtataccaca tttgccccta gatgtgaacg
    14581 agagaaacgc tctagacatt gccaagaagg aaaaaaaagg gtacaatgta acaaaatcaa
    14641 gggaggtctg gaatgaagaa acaaagcaag tacaaagagg tcgtttccta tctgaaaaat
    14701 ggtatcgagt ctggacgatt tccgacggga agtcgcctgc cttctatccg tcaactgagc
    14761 cttgacttcc actgtagcaa ggacaccatt caacgagccc tgctggaatt acggcacgaa
    14821 caatacctct atgccaagcc ccagagtggc tactatgtcc tagaacaagg gcaacaccag
    14881 gacctagaaa tcgaggttac cgacgaacat gccagtgcct atgacgattt ccgactctgt
    14941 gtcaatgaaa ccttgattgg ccgagaaaac tacctcttca actactatga caatcaagaa
    15001 ggattagaag acctaagaca atccattcac aaactcctct ttgagcaagc tctctactgc
    15061 aaggctaacc aactagtact gacttctgga acccaacaag ccttgtttat cctctctcaa
    15121 atatcctttc ctagccaagc caaggaaatc ttggtggaac agccaaccta ccatcggatg
    15181 aatcgcctct tgattgcaca ggggctggac tatcaaacga ttgaacgagg cattgatggg
    15241 attgacttga aggagctgga gcgacacttc aagacaggaa aaattaagtt tttctacacc
    15301 attccccgat ttcactatcc tctgggacat tcctattctg agcaagacaa acgatccatt
    15361 cttaacttag ctgccaagta tgatgtctat atcgtagaag acgattatct gggtgatttg
    15421 gactccaaga agggccaaac cttccactat cttgatacag aggagcatgt catttatatc
    15481 aagtccttct cgaccagcct ttttcctgcc cttcgtatta cagcactcat tcttccaaat
    15541 gctatcaaag aagcatttgt ggcctacaaa aatatcctag actacgacag caacctcatt
    15601 atgcaaaagg ccctgtcact ctatattgac agtcaattgt ttgaaaaaaa tcgtttggct
    15661 cgcttgacca atcatgaatc ttaccaaaaa caaatcgagg aaaggataac taaaacacct
    15721 tgtccccttc ctcattatcc cctacacgat ggtttattgc tagacctgag acagtatcct
    15781 aaaatcgcca gtctcaaaca cagtcaaccg ggcttggact tctttgaaga ggcctattta
    15841 agtacctgtc cttatcaatt tgccaaggtg tccctagaca atctggaaaa tgttttaaac
    15901 tatttaaaag cagaattgga atgacatcca actctgcttt ttcttattct tcaacttcag
    15961 ttagacttgc tgctttttca agatagcttt ggtaaattcc ttcatcctta agtttttgga
    16021 tgaccttgtc taccacttct ttcaaatcat cactattttt tctaagagca acggcattgg
    16081 cgtcgccgtc cttcatcttc aagctgacag ttgcgacagc taagccagcg tttttagcag
    16141 cataactaag tgcaacaggc tcatccatat gaacagcatc tacttttcca gcctgcaatt
    16201 cattgactgc ttcacccata ttagttaggg aagttaattg agcttttggc aattgttcct
    16261 tgaccattga ttctggaaca gtcccttttt gggctgcaat attagcactt tctaggctag
    16321 ttaaatcctt gtatttttcc acatcagcct tacgaaccaa gaaactaatc ttgttttcat
    16381 agtatgggat tgaaaaatca aagacttctt ttctctcgtc agtagcacta attcctgcaa
    16441 ctgctaggtc agccttacca gtttgaagac tggtcaaaac attgtcaaaa ctcatgcttg
    16501 agatttccaa cttaacccca agttcatcag cgatagcctg agccatgtcg atgtctgcac
    16561 cgactacctg gttctttcca tcaaccaatg attgaaattc aaagggtgca tagtcaggac
    16621 tggttgccac aactaatttc cccttttgct taatggcctc aacagctgac tgagaaccat
    16681 tagaacctga ctggcaagct actaagaaga aacttgcaag aaaattacat aaaacaaata
    16741 tccatttttt tgatttcata aataaacaac ctctctgtta attttgtata attataacga
    16801 tatccaagtt acttgtcaag tgttttttaa atttttatct caaaaatatt ttttcgttca
    16861 aaaaaaggag ccatcagttg atttcaagct cctttttata cagaattaaa ctattttata
    16921 gttcgacaat cttacctgtt tcaaagtaga caacccattc acagatattt ttagcatagt
    16981 caccgatacg ctccaagtag gaaataactt ggaaataatc acgacccgta acaatggctt
    17041 ctggattttt cttaatctct tcagtcgcaa ggtcacggat agtttcaaaa tagtggttaa
    17101 tttgctcatc catggaggcc acccggtatg cgtcgtcaac agaaccatta agataaagat
    17161 caagtgctgc ttcaacaacg cttttaactt cacgtcccat tttttttatt tcttcctcta
    17221 cagctggaat acgttcttcc cccttcatac ggatggttgc ctgggcaatg gctacagcgt
    17281 gatcccccat acgctccaca tctgatacag ccttaaggac agtcaagact gtacgcaaat
    17341 cttgagagac tggttgttgg agtgcgatca tttcaaatga tttcttttcc agtttcactt
    17401 cgtattcatt tacttctgca tcatcttcga tgacctcttt tgccaggtca cggtcatgcg
    17461 tgacaaaagc acgtaccgta cgattgattt gtgagagcac ttcttgtccc atagcgtaga
    17521 actggttatg taatttctct aaatcttctt caaattgaga tcgtaacatc tttcatctcc
    17581 ttatccaaat tttcctgtaa tatagtcttc cgtttccttg tgttggggat caaggaacat
    17641 ctgcttggta tcattaaatt caatcaaatc tccatctagg aaaaatcctg tcttatcaga
    17701 gatacgtgaa gcttgctgca tggaacgggt taccagaagc atggtgtact tgtcttttag
    17761 accatacaag gtttcctcaa ttttaccagc tgaaatcgga tccaaagccg aagttggctc
    17821 atccaagagg atgattttag gactagttgc caagacacgg gccacgcaga cacgctgctg
    17881 ttgaccacct gacaatccaa tagctgaatc atatagacga tccttgacct catcccaaat
    17941 agaggcacct tgcaaggctt tttctacggc ttcatccaga acctgcttat ccttaattcc
    18001 attgatacga agcccgtaga caacattctc atagatagtc atagggaaag gattaggttg
    18061 ttggaaaacc attccgattt ccttacgtaa ttcaaccgta tctgtacgcg gactgtagat
    18121 gttgtgacca ttgtacacca cggatccagt tgtggtcacc tctggattga gatctcccat
    18181 gcggttgaga gacttgagga gggttgactt ccctgatcca gatggaccaa tcaaggctgt
    18241 aatttcctta ggttggaaag atagggaaac actattcaaa gccttctttt tattataata
    18301 aacggacagg tctgatacct gtaaaatcgc atctgtcata cggtttcctt tctaaccaaa
    18361 gtgaccagat acatagtcat tggtggactg tagcttggca ttttggaaaa tagttgcagt
    18421 cttgtcatac tcaatcaaat cacccaagta aaagaagcct gtatagtcac ttgcacgagc
    18481 agcctgctgc atattatgcg ttacaatgat gatggtaaag tttttcttga gctcaaacat
    18541 ggtctcttct agttgcatgg tcgcaatcgg atccaaggct gaggctggct catccattaa
    18601 gaggatatct ggcttaacag agatggcacg agcgatacag agacgttgtt gctgaccacc
    18661 tgataaggtc aaggctgact tgtggagatc gtctttaacc tgatcccaga gggcagcctg
    18721 acgaagggag gtttctacga tttcatctag gacttgctta tccttaactc cagcacgttc
    18781 atgcgcaaag gtaatattac ggtaaattga cttagcaaat ggattggggc gttgaaaaac
    18841 cattccaatg tgtttacgca tttcataaac gttgatttct ggacggttga catcaattcc
    18901 acgatagaga atctgcccag ttactttagc aatatcaatg gtatcattca tgcgattgag
    18961 actgcgtaag taggtagatt tccccgatcc cgacggacca atcaaagctg taattttatt
    19021 tctttcaaat tgcatatcaa tccccttaat ggattcattt ttaccatagt aaacatggac
    19081 atccttagta gaaagggcta ctttttcttc aggaaaggta aggatatgct tctcatccca
    19141 gttatatgtt gacatggctt ctcctttagg cagcggttaa tttcttgtgt agatagcttc
    19201 cgaacttacg agctccaaag ttaaaaatca ggataaagat caggagcaca gcggcagaac
    19261 ctgctgatac aatggttcca tctggaatag tgccttcact attgactttc cagatatgga
    19321 cagccaaggt ttctgcttga cggaagatag agatggggct agtcacactg aggatattcc
    19381 agttagacca gtcaagagct ggcgccgatt gccctgctgt atagatcaga gctgcagctt
    19441 cgccaaagat acgaccagat gccaagacga cacccgttac aatacctgga agcgcttctg
    19501 gaataacaac atgaaccact gtctcccagc gagaaatccc aagagccaga ccagcctcac
    19561 gttgggtatg gtgaacgtgt ttcaaactat cctctacatt acgcgtcatc tgaggcaagt
    19621 taaagactgt caaggccaag gcacctgaaa tgattgaaaa tccatactca aactggacta
    19681 caaagatcaa gtaaccaaag agacccacca ccactgatgg taaagaggac aagatttcaa
    19741 tacaagtccg cacaaagttg gtaacaggac cttttttagc atattcagcc aagtaaatcc
    19801 cagctcccat agaaagaggt acagaaataa tcaaggtaat gaccaatagg aaaaaggaat
    19861 tgtaaagctg aatgccaatc ccaccacctg cttgaaaagc agaagacctt ccagtcaaga
    19921 aagaccaaga gatatggggc aagccccgaa ccaagatata gagaatcaag gaagccaaga
    19981 ttgtcacaat gatgctagca atcgtataga ggacagctgt tgcaagttta tctaatttct
    20041 tagcgcgcat aatttttctt tcctctttct ttcgtaatca atttaatcac actgttaaaa
    20101 actaaactca tcaagagcag taccaaggcc agtgaccaga gaacattatt atttacagtt
    20161 cccatgacag tgttcccaat tcccatagtt aatatagaag ttaaagttgc agctggtgtg
    20221 gtcaaggaag ttgggataac agctgagttt ccgacaacca tctggatagc taaagcctca
    20281 ccaaaggcac gcgccatccc aaagaccact gcagtgaaaa taccagaacg ggccgccttc
    20341 aagatcacac gccagatagt ctgccagcga gtggctccca tagcgaaact agcctcacga
    20401 taataacgag gaaccgcacg caagctatcc gttgtcataa aggttacggt cggcaaaatc
    20461 atgacaaaga ggacggaaat ccctgacaaa atcccaaaac cagtcccacc aaagacactg
    20521 cgaacaaagg gaacgacgac ttgcaagcca ataaatccat acactactga aggaatccca
    20581 accaggagtt caatagctgg ttgcaaaatc ttcgcccctt ttggtgatac ttcggtcata
    20641 aaaactgctg caccaatagc aaagggtgtt gcgataaggg ctgagagaat ggtaacgata
    20701 aaggaaccca aaatcatagg aagggcacca aattctttac tagaaggatt ccaagttcct
    20761 cccaaaagaa agtcaaagat attcacacca ttgacaaaga aggtcgacaa gcctttttgc
    20821 gctacgaaaa ccaaaatcat ggccacaagg atgactatca aagaaagaca ggcaaaggtc
    20881 aaaccttttc ctaatttctc cagacgagaa ttctttgatg gaagcaacat tttcttagct
    20941 aattcttctt gattcattat tgtctccctt ccaacactgt cacagttccg gcagcatctt
    21001 tttcaacctt catttcctta atcggaatat acttcaatcc tttgacaatc ccttcttggg
    21061 tctcatccga gagaacaaaa ttgagaaatt ctgcagccaa ctcattgggc tgccccaatg
    21121 tatacatatg ctcataagac cacaagggcc aattattgct acttatattt tctggactta
    21181 agtcatagcc attcaacttc atgcttttga ccgaatcatc tatataggta agagataaat
    21241 aagagatagc tcctggactt tttgatacga ttgattttac cgctccattt gaatcctgct
    21301 cctgactttg catggcagac tgaccttcca taatgacagt atcaaaggta gcacgagagc
    21361 cagagccggc tgcccgattg ataacagaga tgggtaagtc cttaccacca acttctttcc
    21421 aattggttac ctcacctatg aagatttgac gaagttgctc tgtcgttagg ttatcaacat
    21481 caacctcctt attgacaatc agagccaagc cagctaccgc gaccttgtgg tcaacaagag
    21541 cagaagcatc aattccgtct ttttcctcag caaatacatc tgagtttcct atatcaactg
    21601 ccccagactg aacctgggac aagcctgtac cagaacctcc cccttggaca ttgaccgttt
    21661 ttccaacatg gatcgtgcca aattcatctg ccgctacttc aaccaagggt tgcaaggcag
    21721 ttgagccaac agccgttatg gattctccac gatcaatcca gctagcacag cctactaaac
    21781 aagccgtcag ccaaaaagcg ataagagaca gagcaagctt ttttcttttt ttcactgttt
    21841 ttctcctcga aaataattat gaatactgtg aattttttaa gtagttcttt atgagttgac
    21901 gcatgaattc ttaccaaatt tctgcgcaat tgattattta tataatatag gctatattac
    21961 tctttcctaa cctccttttt tcatatgtgg ataaaatctc ttgtctatcc cttccaccat
    22021 tgtcacccat tatagtcatt tcgtgtctct ttttcccctt tttaatgcaa gggaaattac
    22081 tctcctcaga tgataatcca aaagctagaa aggtatctca aacctctcta ctctcccaga
    22141 ctagtttaca actaaaagga aaagattcta ttttatgaga aatctagttt acaagcggta
    22201 agaacgctaa taactaaact tcttgtactc tttgaaaatc tcttcaaacc agtgttttga
    22261 gctatctatg gctagcttcc tagtttgctc tttgattttc atcgagtagt aaaactacat
    22321 gtgatggcaa tcaagatatc aagaatcatc ctactaaaaa aatccatact ttcactataa
    22381 catagaataa gatatttgac tagcattttc atttgaatct gaggcctttt ggaaaataat
    22441 ttttcaaaac atttccagta acctttgcaa agcccaagcc attgccttta accaaaactt
    22501 ggtaccaacc atttggcaga ctttctgcca gctgaacgat ttctccagcc gcatacttga
    22561 caaacgcttc ttggccaatt tcaaccgact gttcgacctg actcggtttc aaggctaaac
    22621 caagagcgaa actgggctca aagcgtttct tcttaaaagt gcccagatgc agtccattgc
    22681 gagcaatctt gagcttccct aaatctggca aaagttctgg caagagataa agctggtctc
    22741 caaaagtctg caagataccc ggtagattga ccttcaaatg gttttgggca aattcctgcc
    22801 acaaggcaac ttgttcacgg ctgaggttac tcttacttgc cttaaattta ggagctggat
    22861 tgttaccctt aaactgtaga tgggcaacaa actgaccctc tcccttaaac tgatgagggt
    22921 acatccgagc cgtttctggc aggtcaatac cagctaccat tccattgata tgctctactg
    22981 gaaacaagtc aaaatcatac tcttccagca accaattgac aatctcttcg ttttcctcgg
    23041 gtgcccaggt acaggtcgaa taaaccagac gaccaccttc agctaacatg gtcactgcat
    23101 cctccagaat ttctctttgc aagctagcac attgactcgg ataatctaag ctccaatagt
    23161 ccatagcatc aggttgctta cgaaacattc cttcaccaga gcaaggggca tcaagaacga
    23221 ttaagtcaaa atagccttta aagaccttga ccaagcggtt ggcagattca ttggtcacca
    23281 cgacatttgt cgctccaaaa cgctccatgt tttcaaccaa aatcttagcc cgtttgcttg
    23341 aaatttcatt ggaaacaagt agcccctccc ctgctagata ggctgccagt tgagttgatt
    23401 tgccccccgg tgcagcagcc aagtccaaga ccttcatacc aggactgggt tgggctactt
    23461 gagccaccat ttgagcagca ggttcttgcg aataaactaa acctgtagca tgctcaggcg
    23521 atttccctga aaccttccca tagtggcccc aaggggtttg agtaatggca tcagaaaagg
    23581 aaagttgctc ttcttttaag ggattgaccc gaaaggccga aaccgcttcc tcctcaaaag
    23641 aggcaagaaa atctcttgcc tcatctccta gtatctcttc atatttttca acaaatcctt
    23701 ctggaaattg catttaagtt cttttccttt cgtaaatata ggactgaatt tcctcctgca
    23761 tctcaagagg tagcatcatg accggttgtc tggtttgaaa atcaggagcc tcaccagata
    23821 gagtcacaac ccgatagccc agactttccc ctaaaatact agccgcagca taatcccatg
    23881 gttgcagata ggtgacatag gtcaataaat gccctgacaa aatcttggca aaactaatag
    23941 ccgcactccc atagacacgt acaccgagga ccgctcgact caaatcagcc agcccccatt
    24001 cgttggtttc cagcatgcca ctatttcccg caatgagaaa atctccaagt ggttttgctt
    24061 gaaagggagc taggagctta tcgtttcggc aaaccggaaa ttccccacca ccatggtaac
    24121 aatccccttt gaccacatca taaatcagac caaactgtcc ctgaccattt tcaaaataag
    24181 ccatcataac agcaaaatct tcctgctggg ctacaaaatt attggtacca tcaatgggat
    24241 caatgaccca aaccttgccc tcttgaaccg aggctcgcag acaaccttct tcagcacaaa
    24301 tcttatcctc aggataacgg gacaaaatct caccaaccaa gagttcctga acttctttgt
    24361 ccagtctggt caccaaatct gttggagagg acttggtttc aacacgcaag tcttcctgca
    24421 tatggtcaag aatgtactga cctgctttct taacaagctc tttagcaaat tcaaatttac
    24481 tttccaagag aaatctttcc ttcccctttt tctttggcag cctgaactgc tcggtaaagt
    24541 gaatagccac taaccgtttc aaattctcgt cccaaacgtt tctcttcgct cttgcttggc
    24601 acgaccgctt tgaatccctt ataggagtcc agtaactttt tagcctctac tttgccttca
    24661 taggcagctt caacatcatt aaaaaaagaa agcactgaag caagttcttc agtgctccac
    24721 gacaaatcta gtgggtaact atactgtttg ttcattaact aataccagct ctcattcttg
    24781 cttcttttag ttcttgctta cgataactac gagggagaaa agcacgaatc tcatcttcat
    24841 taaaaccgat ttgcatacgc ttggcatcaa taataattgg acgacgcaaa agactaggat
    24901 actgctcaat caaatgaagc aattccgata ccgaaatact ctctacatca atattcaatt
    24961 tttgaaaaat ttttgaacga gttgaaatga tgtcatcagt accattttcg gtcaaggaaa
    25021 ggatgtgttg caattctttt cttgttaaag gactggtcat aatattgtgt tccacaaagg
    25081 gaaccttatg tttttctaac caggccttag ccttacgaca tgatgtacag ctcggtgata
    25141 gaaatagtgt aatcatgctt ttctcttctt atctatactt tgctacttct attatacaaa
    25201 aaaataaagc gcttgactag ggatttttag aaaaaaagcc tattttttca agaaaaatag
    25261 gctgtttgcg aacgattgac acaattggat ttggttaatt cactcttaac gatggtttta
    25321 aacgatatat atttttatat atgcaaatta aaaacatctt tcatttcact tcctacgact
    25381 tttcagacac agatagccaa agaagctttc atagagggca aaaaagagga ggaaggcatg
    25441 aagaaagaag gtctctggca aaatcataat aacaggatcc ttggctggat caaaaagcca
    25501 ggtatcatct cccacaaaga gaatttgatg gaaaagagta aagaattggt caaaaccaat
    25561 caaaactccc ccaagtccaa tcatcacagg taagactact agagtcagga gactttttcg
    25621 ataaagagac aaaaagtcct ttttcacaat cctattgaca aagacataga aacttggcag
    25681 tgtcactaga gctactagct gaaccaaatg aaagagattc ttgaccactg cgaaatggtg
    25741 cagaccagct gctgacgaac gaaaatcagg catctgtaag acctgactaa aaggattggt
    25801 cagataattc atcaagatat gaaaattgta ttgaatggtt tctggtttta gatagactcg
    25861 attcgttaag tttagccact gaatctccat aggatagaaa atccaagcca gataaatggt
    25921 cagaaggatt gagagggaga ggagaaagag catagagccc caaaagatca atttagtttt
    25981 catcaaaatc ccactccgca aggctagaaa ccacatgtgt cggtgcgatt ggcaggccag
    26041 ctacttcttc tgccttagta aaacctgtcg tcaccaagag cgttggaatg tcattgtcaa
    26101 tcccagcccg aatatcagtc aaataattgt ccccaaccat gattaactct tcacgttcca
    26161 aacctaagtg ctcaaccgcc ttgtccataa tgatggcatt tggttttccg atataaaccg
    26221 gcttcactcg tgtcgctact tcaagcagcg taatcagtga gccagcacct ggcaaaagac
    26281 cgcgttccgt cgggatgttg aggtcaggat tggttccgat aaaatgggca cccttttgaa
    26341 tagcaagagt tgctgtggca aatttttcat agtcgacttg ccaatccaga ccaactacca
    26401 cgtaggcagg tttttccttg tcttccacat aaccagccgc cttgatggct tccttgagtc
    26461 ctgcttctcc gacgacatag acggtctttt caagccccaa atcattcata tagtcgatgg
    26521 ttgccaaagt cgctgtgtag acagtcgata ggggcgtatc gatattaaaa ttctgagcca
    26581 acatctcctt aacactctct ggagtgcggg ttgtattgtt ggttacaaag agatagggaa
    26641 tgtcccgctt ttgcaattca tgaacaaaag tctctccagc agggattcgg tctttcccct
    26701 tataaatggt tccgtctaaa tcaattaaat agcctttata tttcatctat ttctccctaa
    26761 gcctttttta tttcttgcca agtaatgatt gcttgggcat tgataacccc atcacttgta
    26821 atttcatgct tgctttccag tccagtccgt tcaacagccg atgtaatcac cccacctggt
    26881 cgaacttcct tgacatactt gaggttgatt ttcttgggaa tatagtgggt caaaaaatcc
    26941 gctcccatga cctcaaaaat ccagtccaag tatttactgt tattgacatg accattcata
    27001 tccaagtcgt aaaaacgaac atggtaatcc ttgctgatcg gttcttccaa ggactcatac
    27061 ttcggtccac ggataagttt tttatcaaaa tcagactggt aaggagccac aatctcaggt
    27121 tcaacaacat ggacttttcg actgtcgcgg tccatgagaa caaaggtcgc catcatgtgg
    27181 atgagctcct gctccgcttc attataaata gtaaagcgac ggtagcaaaa aagtcgattg
    27241 tagctcaagg cttccgtttc gatggtaatt tcttccgcaa aacgaggcaa acgaaccacc
    27301 tcaatatcat attctacgat aatccagacc agattatatt cttccaaaat ggccttatca
    27361 ctaactccca gtgcaatcga ctgcatccct gaaacttgca gtgacagcaa aatcacatct
    27421 ggaagtttga tatgaccgtt catatcagcc atatcaaaag gaattttcat tttcatttga
    27481 taagttaagc ccatgatcct actccaaaat aaatcgttct gctacagtat ctcccaaaaa
    27541 gagacctctc tttgtcatgc gaacgtggtc accctcaatc tgcatgaggc cttgttgaac
    27601 caaatctctg acaatttctc cataaagtcc agcaaaagac tgtccaaatt tttcctcaaa
    27661 tcgcgccatg gaaaccccgg atttcttgcg gagtcccaag aacatttctt cttccatttg
    27721 ctccttttga ctcaggtgat cttctgtaat acaagcattg ccttcctcaa ccgcactgag
    27781 ataatgacga atgggaccat gatttttata gcgtactcca ttgacataac cagatgcccc
    27841 tgcaccaata ccatagtatt cagcattgtc ccagtacatg agattgtgac gactttcaaa
    27901 gccgggtttg gagaaattag aaatctcata atgctcaaaa cccgctcgct ccagctctgc
    27961 aatgatgtac tcaaacatct ccgcttctag ttcctcctta ggcagaggca atttcccacg
    28021 tcgcatccgg ttcataaaga ccgtatggtt ttctaaaatc aaactataca aactcatgtg
    28081 gggaatatcc agtccaatgg ctttagccac attttccttt acttgctcca tggtctgacc
    28141 aggcagagca taaatcaaat caatggagat attgtcaaaa ccagccagtt tcaggcgatc
    28201 gatattttca taaatatcct tctccaaatg actgcgccca atttttttca acatcttatc
    28261 atcaaaggtc tgaacaccta gcgaaacacg attgacagcc gaatttttca aaacagctat
    28321 cttatccgca tccaaatcgc ctggattggc ttcaatggtc aactcttcca agacagacaa
    28381 gtccaagttt ttagtcaagc cattcagtaa cacctccagt tgcggagccg acagggctgt
    28441 cggtgttcca ccaccgatat aaagggttga caacttttca atatcataag aacgaaactc
    28501 ttccagcaga tgctctaaat agctgtcgac tggctgattt ttgatgaaga cctttgaaaa
    28561 atcacaataa taacaaatct gggtacaaaa tgggatgtgc acataggctg acgttggttt
    28621 tttctgcata gtaattatta taccacaaag actagattcc agataaaaat caccatcccc
    28681 agatacatag tccgtccgga gatggtgatg gtttattctt ctgttatatc aatcacaatc
    28741 tcttctgagt catcaagagc ttcggctttt tcttgccatt gctccttgag attatttaat
    28801 tgattttttg atgcttctgt cgcttgaaaa gcataggatt taacttgagc aagtatactg
    28861 tccacagtga tttcacctga ctcaatctgt tcttttgttt tcagaacaaa atctgtagcc
    28921 tgctccttaa cttctgtcag tttttcacag acttgctcct tggcatactc cggatcttct
    28981 ctcaaatcat ctagaaaatc ttgagcctga ctgcaaactt gtttgccctt atcacttgtt
    29041 aaaaacaagg caagagctgc acctgaaacg gttcccaaaa ggattgagga taatttaccc
    29101 ataaggattc tcctttttta ttttttgaaa aatttacttg caagacgaag agctgacaga
    29161 cttgcaccag tcttgagtgt ttttgaacca gctgatgaag ctttcttgct caagacacgc
    29221 gcatggtcat tgaggtctga aacagataga gataaatctg caacagcact gaagagtgga
    29281 tcaatcgtag ccaccttgac attgatatca tctgccaaga cattgacctt agccaacaac
    29341 tcattggtgt gatgcaaggt cacatccaca tctgaagtca aggttttaat cgtcttttct
    29401 gtttcatcga tgacacgacc aagcttttgt acagtaatga tcagatagac caaaaagaca
    29461 atcaaagcta gggcaacaag aatatatgca acttctaaca tttagttttc ctcctctgta
    29521 atatagtaag gggccttctt tcgattttga taaataacga tcattatacc gagaccgata
    29581 aggacaactg acagccattg ggacactcga agtccgaaga acatgagact atctgttcgc
    29641 ataccttcga tgaccatacg accgaaacca taccaaatca agtaaaaggc cgtgatatga
    29701 cctcgtctga gactcttcca tttccgtcta aaaatcagaa tcaaggcaaa gccaagcaga
    29761 ttccatagag actcataaag gaaagtcggt tgacggtagc tcccctcaat atacatctgg
    29821 tcacggataa agccaggtag ataatccaga ttatccactg ttgcaccata agcttcttgg
    29881 ttaaagaaat taccccaacg ccccaaactt tgagcaatca taacgctagg cgccgcaata
    29941 tctagaaaat cccaagtatt gatgagttta cggtcagcaa agatatagag cacaagagcc
    30001 ccagttatca aaccaccgta aatggccaaa ccaccattcc aaatggcaaa aatctctcct
    30061 aaattctgac tatagtaatc aaatcggaaa ataacatagt agagacgagc tcctaaaata
    30121 gccaagggaa aagctactaa gataaaatct aaaatatcgt ctggtatgat cttctttcta
    30181 ggtgcttctt tcatggtcaa ataaaccgca agaatcaagc ctgtcacaat acataaggca
    30241 taccaacgaa tggctagggg tcctagttga atagcaattg gatcaagcat tttgcacctc
    30301 atttcgagcg atgagacttg tcagtcgttc gtcgaacaaa cgggtcgcat caaagcccat
    30361 ttccttggca cgataattca tggcagctgc ctcaatcaca acagagatat tacgacctgt
    30421 tttaactgga atacgaatac gaggaatggc tacgccagaa acttcaagtt cctctgcatt
    30481 gtttccaaga cgatcaaagg tcttatgtgt atcgtaattt tccaaataga cagcaagctg
    30541 aacctgtgaa gaatccttga cagcactcgc accgtagaga ctcataacat cgataatacc
    30601 aaccccacga atttcaatca agtgtttcaa aatttcagct ggttcacccc agagagtaat
    30661 ctcatccttg gcaaagatat cgacacggtc atcggctacc aaacggtgac cacgtttgac
    30721 aagctcaaga cctgtctcgc tcttaccaat tccactatct ccctgaatca agacgcccat
    30781 cccataaata tccatcaaga caccgtgcac actggtacgt tctgccaaac gagaatccag
    30841 atagctagat aactctccag ataaacgact ggtagctgca cggctggtta aaatagcaat
    30901 cttacattct ctagcagcct ttaacatctc ctctggaacc accaaaccac gggcaacaat
    30961 gactgctggt gtctcaggta gaaacatttt tttcaaaact tcataacggc tgttggaagg
    31021 catgctgatc agataagacc actccttcat ccccaaaagt tgaatccgct ctggtgtata
    31081 gtagtcaaaa tagcctgtca tttcaagacc aggtcgcgta atatccgctg tattgatttc
    31141 cttttcaagc aattctggtt caccatagac aatgtctagt ctaagctttt caatcacttc
    31201 ttttactaaa accgacatca tttcctcctt caaatgagtt cttctctcta ttatatcaaa
    31261 ttgtaggagg aagtttcaga tttttgcagg ctttggagta tttatttaaa agaaaaagga
    31321 ctagatttct ctagcctttc atttcaatta gaatttttta cgtttacgag ctacttctga
    31381 tttacgttta cgttttacag aaggtttttc atagaattca cgtttgcgtg tttcttgaag
    31441 agtaccagct ttagtaaccg cacgtttgaa acggcgaagt gcgtcgtcaa gagattcatt
    31501 cttacgtact actgttttag acattttttt cactcccttc aagttcaata tctatttcat
    31561 tctacacgta aaaggaataa ttgtcaagca aaaatgcggt ttaattttga ttttttcttc
    31621 catttataca agagttgaaa gcgttcaata gaacattgct tttttatttt tcgagtaagc
    31681 taagcgcttc agcatctgcg atgatggtta catcagggtg attttgaagg ctacttgcag
    31741 gtaggttctc agtcactggg ccagatactg ttccggcaat ggcttctgct ttcgactcac
    31801 cgtaagcaaa aagaataata gacttggcat ccaaaatgtt tttaatcccc attgaaatag
    31861 cttgggttgg gacgtcttca atcttggcaa agaagcgtgc attggcttcg atagtagact
    31921 ggtcaagttc tactagatgc gtttgactgt caaatggagt accaggctca ttaaagccga
    31981 tatgcccatt gcgaccgatt cccaagattt ggaggtcaac tggatggtca gccaaaattt
    32041 ggttgtagcg ttcaacttca gcttcagcat tgtccttaac accacgaggc aagaaacttt
    32101 ctttaaatgg tttttggttg aacaagtttt cttgcatgaa gtaacgataa gactgtggat
    32161 tgtccccatc aaggcccaca tactcatcaa ggttgacact ggttagattt gaaaaatcaa
    32221 ggtcactctc aacaatttcc ttgtaaaatt caagtgggct acttcctgtc gcaagtccta
    32281 gagtttgagc tccattagcc aatttttcct tcaaaatctc aaaagcaact tttccacctt
    32341 gaacttggtt ttcaacttta ataactttca tcttttcatc ctccatttct gtctatattt
    32401 attatatggt atagaccaat ttttgtcaag tgaaaacgat ttaatttcta aaaaaaagag
    32461 cgtccaaact tgaaacgtgt tataatggat aggattagaa cttagaaaga atgaacaaat
    32521 atatgaatac agctgatttt gatttccact tgccagagga attgattgcc caaacgcccc
    32581 ttgaaaaacg tgatgcctcc aaactcctca tcgtcaaccg tgagacagga gaaatgcaag
    32641 ataaacattt ccactctatt attgatatgc tggaatctgg tgatgccctt gtcatgaacg
    32701 acacccgagt tctccctgcc cgcctctatg gtcaaaaagt ggagacagga ggtcatgtgg
    32761 aacttctcct ccttaagaac actagtggag acgagtggga agttctggct aaacctgcca
    32821 aacgcctcaa ggtcggtact cgtatcagct ttggtgatgg ccgcctcagc gctgtcgtta
    32881 cagaagaatt gacccacggg ggacgcattg tccgctttga ataccaagga attttcctag
    32941 aagtcttgga aagtctggga gaaatgcctc tgccacctta tatccacgaa aaattagatg
    33001 accgtgaacg ttatcaaacc gtctacgcca aggaaagcgg ctctgctgca gcaccgactg
    33061 ctggtcttca cttcaccaaa gaactgctgg cagaaatcca agctaagggt gttcatctag
    33121 tctatctgac tctccatgtc ggactcggaa cctttagacc tgtttctgtg gataatctgg
    33181 acgaacacga aatgcactca gagttctatc aactttctga ggaagctgct gccacccttc
    33241 gctctgtcaa aaaaaatggt ggtcgtgtca tcgctgtcgg aaccacttct atccgcacct
    33301 tggaaactat tggttccaag tttgatgggc aaatccaagc agattctggt tggaccaata
    33361 tctttatcaa acctgggtat gagtggaagg tcgtggatgc cttctcaacc aacttccacc
    33421 tgccaaaatc aactctggtc atgttggttt ctgcctttgc aggccgtgaa ttagtcttag
    33481 atgcctacca ccattccatc caagaacact accgcttctt cagttttggt gacgccatgt
    33541 ttatttattg agaaagaatt tctctaaatc ttctaatacc aataaatcgc taagatatta
    33601 tttcaaagaa catctacaat tgaaactcta gctagctgtg gaagaggcct agtacattga
    33661 aattaaaatg cttcccccta gcttcgaaaa tattgccata gattgcgttg actctccaaa
    33721 ttgattcatc tatattttat ttcagcttcc tatattttct tcgctgtttg taaatcaaaa
    33781 tgcaagacac atgagtagca ccatatttgt tactcttatc tgtcctctca agagactatt
    33841 atgagttatt tcagaatcat tcactacttt gaccctgact ctccttagtc tcaaaatcaa
    33901 agacttatac tcttcaaaaa tctcttcaaa ccgcgtcaac gtcaccttgg attatatatg
    33961 tgactgactt cgtcagttct atctacaacc tcaaagcagt actttgagca acctgcgact
    34021 agttttctag tttgctcttt gattttcatt gagtattaaa caaaaagtga acaaatctga
    34081 attctaatgt acagaagact aggcttgttc acttttttat agtcgctata agatgacctt
    34141 atctatagct ttttatatat aattatatat tcagacatac tattatcaat tttgtcgcag
    34201 ggaggaatct gttaacgcac ccattcacca ttatcattga ctctatagcc atctatactt
    34261 gtattgaccg ctaactcacc cgatgcattt acataatacc atttaccacc aacttggaac
    34321 cattgattga ctttcataga accgttgctg ttgaggtagt accatgaacc attaacttgt
    34381 acccaacctg ttgccatgga accatcagta ttataaaaat accacatacc attttcttgt
    34441 ttccagtctg ttgttggagc aactgcttta gctggttcta ctgctacatc tgttccttgg
    34501 ttagatgtaa cagatacagg atacgaagga atagatgatt gctcaggaac aacaactttt
    34561 tcaggttctc tcgtccctct ccttatacgt ctttttacca tctctttagt aatttgacga
    34621 gaagtagttt cttcaattgt tccatcacgt tcatctacag catagattgt agtaagagta
    34681 atttaccaat ttctcctact tcttctactt cttgactttt atcaagagtt gggccatcga
    34741 gatattctgt ttcgattgga atttcttgga caagaacttg gggcttggtt ctttttttaa
    34801 caactcttgt ttgagagtct tttttttgac ttaaagtact ctcagttact tgtccactct
    34861 ttccatctac attataagtt atcgttgtaa ctgttttccc attctttcct agagtaatct
    34921 cttgctcctg tcctgcagaa aggtcattgt ctgcttcata tttagtagca aatggaacaa
    34981 gaacttcttc aaccttgctt ttagctggaa ctttgataac tgtatccgtg gcttcttttc
    35041 tatcaacagt aacctgttcg gtaacataac cagtctctgg attaacatcg taggtccttg
    35101 tcgtagttac atagccatcc tctccatcaa ttgtaacagg attttcacta cggtcttttg
    35161 tttcatcttt ttcataacga attcgcgtac ttgaaatttt cttggttact accttaggtt
    35221 tagtcgctac ttttacaata atatccccat tgtcagcgtc atcatactct attccctctt
    35281 ctttatctct agtatcatct ctgacatatt gaatcccatc agcagcatga acaaaacttg
    35341 tattcagatt cctcctaaaa ataaagttag cccgattacc gcagaaccaa aaatctttcc
    35401 gagtttacgt attgcatagc gcttattagt attagatttt gccattacat cctacttcta
    35461 gtatagcatc ttttct
//
LOCUS       DAVKZB010000009        33547 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKZB010000009 DAVKZB010000000
VERSION     DAVKZB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.009"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..316
                     /locus_tag="VXA80_000410"
     CDS             <1..316
                     /locus_tag="VXA80_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352957.1"
                     /translation="YSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQNSD
                     DKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKLEI
                     SR"
     gene            372..1067
                     /locus_tag="VXA80_000411"
     CDS             372..1067
                     /locus_tag="VXA80_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0352958.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1079..1495
                     /locus_tag="VXA80_000412"
     CDS             1079..1495
                     /locus_tag="VXA80_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0352959.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
     gene            complement(2122..2214)
                     /locus_tag="VXA80_000413"
     CDS             complement(2122..2214)
                     /locus_tag="VXA80_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV0352960.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            2375..3346
                     /gene="ruvB"
                     /locus_tag="VXA80_000414"
     CDS             2375..3346
                     /gene="ruvB"
                     /locus_tag="VXA80_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV0352961.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            3327..3899
                     /locus_tag="VXA80_000415"
     CDS             3327..3899
                     /locus_tag="VXA80_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV0352962.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLKKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            4130..4888
                     /locus_tag="VXA80_000416"
     CDS             4130..4888
                     /locus_tag="VXA80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV0352963.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            4897..5700
                     /locus_tag="VXA80_000417"
     CDS             4897..5700
                     /locus_tag="VXA80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV0352964.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIVAMLAMHE
                     LLKMRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            5722..6981
                     /gene="rseP"
                     /locus_tag="VXA80_000418"
     CDS             5722..6981
                     /gene="rseP"
                     /locus_tag="VXA80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV0352965.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKCINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAARNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            6994..8847
                     /locus_tag="VXA80_000419"
     CDS             6994..8847
                     /locus_tag="VXA80_000419"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV0352966.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            8946..10325
                     /gene="bglA"
                     /locus_tag="VXA80_000420"
     CDS             8946..10325
                     /gene="bglA"
                     /locus_tag="VXA80_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0352967.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            10517..12325
                     /gene="glmS"
                     /locus_tag="VXA80_000421"
     CDS             10517..12325
                     /gene="glmS"
                     /locus_tag="VXA80_000421"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV0352968.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMVIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(12402..12539)
                     /locus_tag="VXA80_000422"
     CDS             complement(12402..12539)
                     /locus_tag="VXA80_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352969.1"
                     /translation="MKIKEQTRKLATGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            12596..13645
                     /locus_tag="VXA80_000423"
     CDS             12596..13645
                     /locus_tag="VXA80_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV0352970.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(13745..17515)
                     /gene="spuA"
                     /locus_tag="VXA80_000424"
     CDS             complement(13745..17515)
                     /gene="spuA"
                     /locus_tag="VXA80_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV0352971.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNYRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGDRGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDTASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            17932..18345
                     /gene="rpsL"
                     /locus_tag="VXA80_000425"
     CDS             17932..18345
                     /gene="rpsL"
                     /locus_tag="VXA80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV0352972.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            18365..18835
                     /gene="rpsG"
                     /locus_tag="VXA80_000426"
     CDS             18365..18835
                     /gene="rpsG"
                     /locus_tag="VXA80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV0352973.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            19260..21341
                     /gene="fusA"
                     /locus_tag="VXA80_000427"
     CDS             19260..21341
                     /gene="fusA"
                     /locus_tag="VXA80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV0352974.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            21445..25836
                     /locus_tag="VXA80_000428"
     CDS             21445..25836
                     /locus_tag="VXA80_000428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV0352975.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEAIYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFINKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            25938..26201
                     /locus_tag="VXA80_000429"
     CDS             25938..26201
                     /locus_tag="VXA80_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV0352976.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            26194..26472
                     /locus_tag="VXA80_000430"
     CDS             26194..26472
                     /locus_tag="VXA80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV0352977.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWFLVYKVDKSELILNLLRTGSHSDLF"
     gene            26493..26645
                     /locus_tag="VXA80_000431"
     CDS             26493..26645
                     /locus_tag="VXA80_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0352978.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            26667..27908
                     /locus_tag="VXA80_000432"
     CDS             26667..27908
                     /locus_tag="VXA80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV0352979.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLYAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            27918..28148
                     /locus_tag="VXA80_000433"
     CDS             27918..28148
                     /locus_tag="VXA80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0352980.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            28371..29093
                     /locus_tag="VXA80_000434"
     CDS             28371..29093
                     /locus_tag="VXA80_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0352981.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEIFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            29310..30644
                     /gene="pepC"
                     /locus_tag="VXA80_000435"
     CDS             29310..30644
                     /gene="pepC"
                     /locus_tag="VXA80_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV0352982.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(30700..31611)
                     /locus_tag="VXA80_000436"
     CDS             complement(30700..31611)
                     /locus_tag="VXA80_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0352983.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(31635..32438)
                     /locus_tag="VXA80_000437"
     CDS             complement(31635..32438)
                     /locus_tag="VXA80_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV0352984.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(32466..33455)
                     /gene="manX"
                     /locus_tag="VXA80_000438"
     CDS             complement(32466..33455)
                     /gene="manX"
                     /locus_tag="VXA80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV0352985.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         9592 a         6418 c         7831 g         9706 t
ORIGIN      
        1 ttattcgcag gtaaaagttg tagataaact tactgattct gaatcgaaaa aatacggtga
       61 atatattgat ttgtacgaat cgtgtcgtca gaacaattta agtgttctac ttaactttct
      121 tcaaaattcg gatgataaac gaggagttat tgctagtaac tcagtgagaa ttatgtttga
      181 atctttggat aaagcagacc aaattgcggc cctgaatatt tgtagaaact atgtagcacg
      241 agaaaatgtt ttaaataaag aagatgcaga aaaattgtta aatgaaattg ataaaaaact
      301 tgaaattagc agataggaca attcaaatag agttgagtaa aaagtttgaa aagtgaaaca
      361 aggagaaagc tatgccagta aatgaatatg gtcagatgat tggtgagtca atggaaggtt
      421 atacacccgg tgcactgcct tctattgatt tcttagaagg gcgttatgct aggatagagg
      481 ctctctcggt agaaaaacat gcggaggatt tgctagctgt ttatggcccg gatactcctc
      541 gggagatgtg gacctatctt tttcaggagc cagtagcaga tatgggggag ctggttagcc
      601 ttttaaatca gatgttggct cgtaaggacc gtttttacta tgcaatcata gacaaggcga
      661 ctggtaaggc tttgggaact ttttctctca tgcgcattga ccagaataac cgagtaatag
      721 aagtcggagc agtcactttt tctccagagc taaggggtac acggatagga actgaggctc
      781 agtatctcct agctcgctat atttttgagg agctaaacta tcgtcgctac gagtggaaat
      841 gcgatgcctt aaatctgcca tccagacgag ttgcggagcg tttgggattt gtctatgaag
      901 gaaccttccg ccaggcggtg gtttataagg ggcgtacgag agatacggat tggttgtcta
      961 tgattgatat ggactggcct caagtcaaag atcgtttgga aacatggctg cgtcctgaaa
     1021 attttgataa aaatggacag cagtacaaga gcttgagaga actttaagag gtgttgagat
     1081 gattactatt aaaaagcaag aaattgtcaa gctagaggat gttttgcatc tctatcaggc
     1141 tgtcggttgg acaaattata cccatcaacc agagatgctg gagcatgcct tatcgcattc
     1201 attagtaatt tatctggcac ttgatggtga tgctgtggtg ggcttgattc gtttggttgg
     1261 agatggattc tcatcagttt ttgtacagga tttgattgtt ttaccaatct atcagcgtca
     1321 agggattggt agtgccctaa tgaaagaggc cttaaaagat tacaaagatg cttatcaagt
     1381 ccagctggtg acagaagaga cagaaaaaaa cgtgggattt tatcgttcta tgggctttga
     1441 aatcttatcc acctataatt gtatagggat gacttgggcg aatcgagaaa aataacaaaa
     1501 cttgtttttt cttaagcaaa atttaaggat ggtctagtat tatataatca ttaaataaag
     1561 acctcctaac tttatttaat aaaatcctaa acttttcttt ttcataataa tctcccttaa
     1621 ctccacccaa tcaggtggag ttttttagct ctatttcagg cttttgggga ctattctaaa
     1681 aataattttt cgatattttt cggtattttt cggattttgg tcggggaatt ggcggggact
     1741 tttttagcga atataactaa gaaataggtc tgtggtcgct tcagctagtt caaactcaat
     1801 ttgattgtaa gtgactgtta attgaagaac tggaactact gtaataatga ttttgcttgt
     1861 ctagtagtgg cgtattaaat gagaagtgac atgtaagatt gtctcttcgt ttacgaaatc
     1921 aaaatttcta tgaaattgat agaatcccta acgttctctt aatcttaacc gtcttgtcgt
     1981 acagcggttg aagaaaatgt aaacctttga caaaattcgt gaactatggg ataataaaaa
     2041 ggaattgagt actagttcta tatcataggc tagaaaagac cccaagctca cgaccaaata
     2101 agcttgaggt cttttttgac actatttgtc cttgtttagc catttatcga ctatacgaag
     2161 aacgacaccg accacaattg gtccgatgat agttttgagt actaattcca tcatgggcta
     2221 tctcacctcc tttcgtaggc ggtgtagcag tgtcgtagaa tattatacca cataagtgct
     2281 aaacttagga gtcacccaat caggtggctt ttttgtttgt ggcttggttt tttgatataa
     2341 tagagccatg agtagaattt tagataatga gataatgggg gatgaggagt tagtagaacg
     2401 cacgctccgt cctcagtatt tacgtgaata tatcggacag gataaggtca aggaccagct
     2461 acaaatcttt attgaagctg ccaaaatgcg ggatgaagcg ttggatcatg tgctcttatt
     2521 tgggcctcca ggtctcggga aaacgaccat ggcctttgtt attgccaacg aactgggagt
     2581 caatcttaag cagacgtcgg gtccagtcat tgaaaaagcc ggagatctgg tagctatttt
     2641 gaatgagtta gagcctgggg atgtactttt tattgatgag atccatcgtt tgccaatgtc
     2701 agtggaagag gtgctttata gtgctatgga ggacttctac atcgatatta tgattggggc
     2761 tggtgagggt agtcgtagtg ttcatttgga gttaccacct tttaccttga ttggtgcgac
     2821 gactcgggct ggtatgctct ccaatccgct acgggcacgt tttgggatta caggccatat
     2881 ggagtattat gcccatgctg acttgacaga aattgtcgag cggacggcag atatttttga
     2941 gatggaaatc actcatgagg cagcatctga gttggcccta cgtagtcgtg ggacccctcg
     3001 tattgccaat cgtctcctca agcgcgtgcg cgattttgcc cagataatgg ggaatggggt
     3061 aattgatgat attattaccg ataaggcttt gactatgctg gatgttgacc atgaaggttt
     3121 ggactatgtg gatcaaaaaa tccttcgtac catgattgag atgtacagtg gaggacctgt
     3181 tggtctagga actctttctg tgaatatcgc cgaagagcgt gagacagttg aagacatgta
     3241 tgagccttac ttgattcaaa aaggttttat catgcggaca cggtctggac gggtggcgac
     3301 tgctaaggca tatgagcact taggttatga atacagtgaa aaataagcaa gaaatcttag
     3361 aggcttttag agaaaatcca gatatgatgg ccattctgac gatcatccga gaccttggtc
     3421 tgaaagactc gtggttggca gcaggttctg tcagaaattt catttggaat cttttgtcag
     3481 acaaatcccc ttttgatcat gaaacagata tagatgtgat tttctttgat ccagattttt
     3541 cttatgagga aaccttatta ctgaagaaaa agctgagaga ggattttcct cagtaccagt
     3601 gggaattgaa aaatcaggtc tatatgcatc agcacagtcc tcacactgct tcctatacca
     3661 gttctcgtga tgctatgagt aagtatccag aacggtgtac ggcagttgga ctgcgcttga
     3721 atgaagaatc agattttgaa ctctatgtac cttatggttt ggaggatatt ttgaattttc
     3781 aagttcgtcc aactcctcat ttcttagaaa atgaagaccg aatggaactc tatcaaacac
     3841 gtttatccaa gaaaaattgg caggagaaat ggaaaaattt gatttttaaa aatacttaag
     3901 gaaactttaa gctagggagt gtacactaag ttcataagtt aagaagacct taacttaaac
     3961 tcctaaaact ttttcataat aatctcccta taaaaataaa gtcgcccaat caggcggctt
     4021 atttttttga aaaatgggct tggtgcctga gaataaatag cttagtgata gaagaaaatg
     4081 gggaaatatg gtataatgaa acgatagatt tttgaatagg aataagatca tgtttggatt
     4141 ttttaagaaa gataaggctg tggaagtaga ggttccgaca caggttcctg ctcatatcgg
     4201 catcatcatg gatggcaatg gccgttgggc taaaaaacgt atgcaaccgc gagtttttgg
     4261 acataaggcg ggcatggaag cattgcaaac cgtgaccaag gcagccaaca aactgggcgt
     4321 caaggttatt acggtctatg ctttttctac ggaaaactgg acccgtccag atcaggaagt
     4381 caagtttatc atgaacttgc cagtagagtt ttatgataat tatgtcccgg aactacatgc
     4441 gaataatgtt aagattcaaa tgattgggga gacagaccgc ctgcctaagc aaacctttga
     4501 agctttaacc aaggctgagg aattgactaa gaacaacaca ggattgattc ttaattttgc
     4561 tcttaactat ggtggacgtg ctgagattac acaggcgctt aagttgattt cccaggatgt
     4621 tttagatgcc aaaatcaatc caggtgacat cacagaggaa ttgattggta actatctctt
     4681 tacccagcat ttgcctaagg acttacgaga cccagacttg attatccgta ctagtggaga
     4741 attgcgtttg agcaattttc ttccatggca gggagcctat agtgagcttt attttacgga
     4801 caccttatgg cctgattttg acgaagcggc cttgcaggaa gctattcttg cctataatcg
     4861 tcgtcatcgc cgatttggag gagtttagga ggaaatatga cccaggattt acagaaaaga
     4921 accttgtttg cagggattgc cctggctatt ttcctaccaa ttttaatgat tgggggcctc
     4981 ttgcttcaga tagcaatcgg aatcgtagcc atgctagcca tgcatgaact tttgaagatg
     5041 agaggtctag cgaccatgac gatggagggc ctcttgaccc tctttgcaac ctttgcattg
     5101 accattccct tggagaatta cctgactttt ttgccagttg atgggaatgt ggttgcctat
     5161 agtgttttga tttcaatcat gttaggaacg accgttttta gcaagtctta tacgattgag
     5221 gatgcggttt tccctcttgc tatgagcttc tacgtgggct ttgggtttaa tgctttacta
     5281 gatgctcgtg ttgcaggttt ggacaaggct ctcttagcct tgtgtatcgt ctgggcgaca
     5341 gacagtggtg cctatcttgt tgggatgaac tatgggaaac gaaagttagc accaagggta
     5401 tcgcctaata aaacccttga gggtgccttg ggtggtattt taggagcaat tttagtaacc
     5461 attatcttta tgatagttga cagtacagtt gctcttccat atggaattta caagatgtca
     5521 gtctttgcta ttttctttag cattgctgga caatttggtg atttactaga aagttcgatc
     5581 aaacgtcatt ttggtgttaa ggattctggg aaatttatcc ctggacatgg tggtgttttg
     5641 gatcgtttcg atagtatgtt gcttgtattt ccaatcatgc acttatttgg actcttttaa
     5701 tcaaaagacg gaggaaacgc tatgctcgga attttaacct ttattctggt ttttgggatt
     5761 attgtagtgg tgcacgagtt cgggcacttc tactttgcca agaaatcagg gattttagta
     5821 cgtgaatttg ccatcggtat gggacctaaa atctttgctc acattggcaa ggatggaacg
     5881 gcctatacca ttcgaatctt gcctctgggt ggctatgtcc gcatggccgg ttggggtgat
     5941 gatacaactg aaatcaagac aggaacgcct gttagtttga cacttgctga tgatggtaag
     6001 gttaaatgca tcaatctctc aggtaaaaaa ttggatcaaa cagccctccc tatgcaggtg
     6061 acccagtttg attttgaaga caagctcttt atcaaaggat tggttctgga agaagaaaaa
     6121 acatttgcag tggatcacga tgcaacggtt gtggaagcag atggtactga ggttcggatt
     6181 gcacctttag atgttcaata tcaaaatgcg actatctggg gcaaactgat taccaatttt
     6241 gcaggtccta tgaacaattt tatcttaggt gtcgttgttt tttgggtttt aatctttatg
     6301 cagggtggtg tcagagatgt tgataccaat cagttccata tcatgcccca aggtgccttg
     6361 gccaaggtag gagtaccaga aacggcacaa attaccaaga ttggctcaca tgaggttagc
     6421 aactgggaaa gcttgatcca agctgtggaa acagaaacca aagataagac ggcaccgact
     6481 ttggatgtga ctatttctga aaaggggagt gacaaacaag tcactgttac acccgaagat
     6541 agtcaaggtc gttaccttct aggtgttcaa ccgggggtta agtcagattt tctatccatg
     6601 tttgtaggtg gttttacaac tgctgctgac tcagctctcc gaattctctc agctctgaaa
     6661 aatctgattt tccaaccgga tttgaacaag ttgggtggac ccgttgctat ttttaaggca
     6721 agtagtgatg ctgctagaaa tggaattgag aatatcttgt acttcttggc aatgatttcc
     6781 atcaatattg ggatttttaa tcttattccg attccagcct tggatggtgg taagattgtg
     6841 ctcaatatcc tagaagccat ccgccgcaaa ccattgaaac aagaaattga aacctatgtc
     6901 accttggccg gagtggtcat catggttgtc ttgatgattg ctgtgacttg gaatgacatt
     6961 atgcgactct tttttagata atcgaggaat attatgaaac aaagtaaaat gcctatccca
     7021 acgcttcgcg aaatgccaag cgatgctcaa gttatcagcc atgctcttat gttgcgtgct
     7081 ggttatgttc gccaagtttc agcaggtgtt tattcttatc taccacttgc caaccgtgtg
     7141 attgaaaaag ctaaaaacat catgcgccaa gaattcgaaa agattggtgc tgttgagatg
     7201 ttggctccag cccttcttag tgcagaattg tggcgtgaat caggtcgtta cgaaacctat
     7261 ggtgaagacc tttacaaact aaaaaaccgt gaaaaatcag actttatctt aggtccaact
     7321 cacgaagaaa cctttacagc tattgtccgt gattctgtta aatcttacaa gcaattgcca
     7381 ctcaaccttt atcaaattca gcccaagtat cgtgatgaaa aacgcccacg taatggactt
     7441 cttcgtacac gtgagtttat catgaaggat gcttatagtt tccacgctaa ctatgatagt
     7501 ttggatagtg tttatgatga gtacaaagca gcctatgagc gtattttcac tcgtagtggt
     7561 ttagacttca aggctattat tggtgacggt ggagccatgg gtggtaagga tagccaagaa
     7621 tttatggcca ttacatctgc tcgtacagac cttgaccgct gggttgtctt ggacaagtca
     7681 gttgcctcat ttgacgaaat tcctgcagaa gtgcaagaag aaatcaaggc agaattgctc
     7741 aaatggatag tctctggtga agataccatt gcttactcaa gtgagtctag ctatgcagct
     7801 aacttagaaa tggcaacaaa cgagtacaaa ccaagcaacc gtgttgtcgc tgaagaagaa
     7861 gttactcgtg ttgcaacgcc agatgttaaa tcaattgatg aagttgcagc cttcctcaat
     7921 gttccagaag aacaaacgat taaaaccctc ttctacattg cagatggtga gcttgttgca
     7981 gcccttctag ttggaaatga ccaactcaac gaagtcaagt tgaaaaatca cttgggagca
     8041 gatttctttg acgttgctag cgaagaagaa gtggcgaatg ttgttcaagc aggatttggt
     8101 tcacttggac cagttggttt gccagagaat attaaaatta ttgcagatcg taaggtacaa
     8161 gatgttcgca atgcagttgt cggtgctaac gaagatggct accacttgac tggtgtgaac
     8221 ccaggccgtg attttactgc agaatatgtg gatatccgtg aagttcgtga gggtgaaatt
     8281 tccccagatg gacaaggtgt ccttaacttt gcgcgtggta ttgagatcgg tcatattttc
     8341 aaactcggaa ctcgctattc agcaagcatg ggagcagatg tcttggatga aaatggtcgt
     8401 gctgtgccaa tcatcatggg atgttacggt atcggtgtca gccgtcttct ttcagcagtg
     8461 atggagcaac acgctcgcct ctttgttaac aaaacgccaa aaggtgaata ccgttacgct
     8521 tggggaatca atttccctaa agaattggca ccatttgatg tgcatttgat tactgtcaat
     8581 gtcaaggatg aagaagcgca agccttgaca gaaaaacttg aagcaagctt gatgggagct
     8641 ggttatgaag tcttgacaga tgaccgtaac gaacgtgtcg gtgttaaatt tagcgatagt
     8701 gacttgattg gtcttccaat ccgtatcact gttggtaaaa aagcggccga tggcattgta
     8761 gaagttaaga tcaaggcaac tggtgacacc attgaggttc atgcagataa cgtgcttgaa
     8821 acgcttgaaa tcctaagcaa gaaataaaaa ctataatcag aagaaaaaca aggaaaaaat
     8881 gtaactagtt tttaccttgt tttttctgta taatgggaga aatgagtaga taaagaggta
     8941 aatgtatgct aagatttcca aaggattttg tctggggatc ctctacttct ggaccgcaaa
     9001 cagaaggacg tgtagctggt gacggtaagg gagacaatct ctgggattac tggttccaag
     9061 tggagccaaa tcgttactat aatgggattg gttcagataa gacatcgact ttttatgaaa
     9121 attgggagcg ggatattgag cttttgttag agactggtca cacagccttt cggacttcta
     9181 ttcagtggtc acggattttt ccacaaggct gtggaaaagt caaccctcaa ggtgtggatt
     9241 tttatcgtaa ggtctttgag gctattaagg ctaaaggaat tcgtctgtta gtcaatctct
     9301 atcattttga tttacctttt gcccttcaag aggatggtga tggttgggaa aataaggcga
     9361 cagtctcagc ctatgaagac tatgctcgtt tttgttttga gacttatgga gatttagtgg
     9421 atcaatggat tacctttaac gagcccatcg ttcctgtaga atttggttat ttttatgatg
     9481 cccattatcc acataaggtg gatgcagagg cagctgttaa ggtagcctat catacacaat
     9541 tggccagcag ccgggctgtt aaggcttgcc atgaactttt gcctgattcc aagattggga
     9601 ttgtcctcaa cttgacaccg gcttatccac gtagccagca tcctgctgat gtcaaggcag
     9661 ctcgtattgc ggcccttttt caggcccaat ctttcttaga tccatctgtc ttgggaactt
     9721 atccacagga gttagtagaa atcttgcatg aacacggtct tttacctgat gctacagagg
     9781 aagagttgga actcattcgt gataatacgg tggacttcct tggtgtgaac tactatcaac
     9841 ctttgcgtgt tatggctcct cgatttgcta agcatccaga gagtccactc ttaccagaac
     9901 atttttacga gccttatgtg atgcctggac gtaaaatcaa ttctcaccgt ggttgggaga
     9961 tttatgagca agggatttat gacatcgccc aaaatatcaa ggaaaattat ggcaatattg
    10021 agtggatgtt gactgagaat ggtatgggtg ttgaagggga agaaaaattc cgtcaagatg
    10081 gaatgattca agatgattac cgtattgact ttgtaaaagg tcatcttcgt gaacttcacc
    10141 gtgccattga agatggtgcc aactgtaagg gctacttgat ttggaccttt attgactgct
    10201 ggtcatggct caacagctat aaaaatcgct atggtttggt cgaattagac ttggaaacgc
    10261 aagaacgtcg tctgaagaaa tcaggccact ggttcaaaga attaagcgat aataatggat
    10321 tttaaaaagg actctgactg attatcccaa tcggtcagag ttttttgctt acaggagctt
    10381 aatttgaact ataccaattt ttactcttga caagtgaaag aaacaagtat atactgtttt
    10441 tgctgattct ataaaagatg aattagacta taccaatttt aaggagaaag cacagcttgc
    10501 ctgtgtcgta tatactatgt gtggaattgt tggtgttgtt ggaaacacaa atgcaactga
    10561 tattttgatt caagggcttg aaaagcttga ataccgtggc tatgattctg cgggaatttt
    10621 tgtcctagat ggtgctgata accatttggt gaaggctgtt ggtcgtatcg cagaattgtc
    10681 tgccaagaca gctggtgttg agggaacaac tggtatcgga catactcgtt gggcaactca
    10741 tgggaaacca acggaagaca atgctcaccc acaccgatct gagacagaac gttttgtctt
    10801 ggttcacaat ggggtaattg aaaactacct tgaaattaaa gaaaaatacc ttgctgggca
    10861 ccacttcaaa gggcaaacag atacggaaat cgccgtacat ttgattggaa aatttgcgga
    10921 agaagacggt ctctcagttc ttgaagcctt taaaagagct cttcatatta tccgtggttc
    10981 atatgccttt gccttgattg actctgaaaa tccagatgtc atctatgtag cgaaaaacaa
    11041 atctccactt ttgattggtc ttggggaagg ctacaatatg gtctgctcag acgccatggc
    11101 tatgattcgt gaaactaacc aatacatgga aatccatgac caagagttgg taatcgtcaa
    11161 ggctgatagt gtggaagttc aagactatga tggaaatcgt cgtgagcgtg ctagctacac
    11221 tgctgaactt gacttgtcag atatcggtaa gggaacttac ccttactaca tgctcaagga
    11281 aattgacgag caaccaactg ttatgcggaa actcatccaa gcctacacag atgaggctgg
    11341 tcaagttgtc gtagacccag atatcattaa ggctgttcaa gacgcagacc gcatctacat
    11401 ccttgcagct ggaacatctt accatgcagg atttgcttct aagaaaatgt tggaagaatt
    11461 gacagataca ccagttgaac ttggaatctc atctgagtgg ggctacggta tgccacttct
    11521 cagcaagaaa ccactcttca tttttatcag ccaatctggt gaaacagcgg atagtcgtca
    11581 agttttggtc aaggctaatg aaatggtaat tccaagctta acagtgacaa acgttccagg
    11641 ttcaaccctc tcacgtgaag ccaactatac catgctcctt cacgcaggtc ctgaaattgc
    11701 cgtggcatca actaaagcct atacagcgca aatcgcagcc cttgccttcc ttgcaaaagc
    11761 agtcggagaa gcaaatggta atgctaaagc gcaagccttt gacctggttc atgaattgtc
    11821 aatcgtagct cagtctatcg aatcaactct ttcagagaaa gaaaccattg aagccaaggt
    11881 tcgtgaactt cttgaaacaa ctcgtaacgc cttttacatc ggacgtggtc aagattacta
    11941 cgtagccatg gaagcaagtc tcaaactcaa agagatttct tatatccagt gtgaaggttt
    12001 tgcggcagga gaactcaagc acggaaccat tgccttgatt gaagaaggaa cgcctgtttt
    12061 ggctctcttg tcagatccag ttcttgccaa ccatactcgt ggaaatatcc aagaggtcgc
    12121 agcccgtggt gccaaagtcc tcactatcgc agaagagaat gtagccaaag ataccgacga
    12181 tatcgtcctt acgaccgtac atccatacct ctcaccaatt tcaatggtcg taccaacgca
    12241 attagtcgct tactttgcaa ccctccaccg tggcctcgat gtggataaac cacgtaacct
    12301 tgctaaatca gtaacggtag aataagctaa aaaagtctag ttatctaggc tttttctaat
    12361 gagcaaattg gttgcttgta cttaagattc gtgttatact cttagaaaat ctcttcaaac
    12421 cacgtcaacg tcgccttgcc gtaggtatgg ttactgattt cgtcagttct atccacaacc
    12481 tcaaaacact gttttgagca acctgtggct agcttcctag tttgctcttt gattttcatt
    12541 gagtattaga ataatttttc aaattgaagg acagagatag acaaggagaa tcacaatggt
    12601 agaattggga atttcaacat ttggggaaat aacggagctt gaagggactg gacaaactta
    12661 cagtcatgct gaacgcattc gccagttggt ggcagagatt gagctggctg acaaggttgg
    12721 tttggacgta tatgggattg gcgagcacca tcgggcggat tttgcagtat cagccccaga
    12781 gattgtcctg accgctgggg cagtcaatac caagaaaatc cgtttgacca gtgcagtcag
    12841 cattctgtca agcatggatc cgattcgttt gttccaacag tatgccacta tcgatgcttt
    12901 gtcaaatgga cgagcggaga ttatggctgg aaggggctct ttcacggaat ctttccctct
    12961 gtttggttat gacttgaaag actacgactc tctttttgat gagaaattag acttgcttca
    13021 gttagtcaat gaaaagacca agttagactg gcaaggtcga ttgactcaaa cgattgctgg
    13081 taaagaagtt tatcctcgtc cagttcagga caaattgccc ttgtggatag ctacaggtgg
    13141 tcatgtcgaa tcaacagtga agattgctca ggctggtcta ccgattgtat atgctattat
    13201 tggtggtaat ccacgttatt ttaaaaagtt gattcaggct tatcgtgaga ttggaagcga
    13261 agcgggccat gctgacaaag acttgaaagt gggagcccat tcttgggggt ggattgcgga
    13321 agatggtgag caggcggtga aagattattt ccatccgact aagcaagtgg tggatgctat
    13381 ttccaaagac cgtccgcact ggcaggaatt gcgttatgag caatatttgg agcaggtagg
    13441 gccaaatggc gccatgtttg ttggcaatcc agatcaggtg gccgaaaaat tgattcgcat
    13501 gatagaggat ttaggattgg accgcttcat gcttcatcta ccgcttggtt ctatgcctca
    13561 tgaccaggtt ctgagagcta ttgaactctt cggaacacaa gttgcaccca aagtacgagc
    13621 ttattttgct atgaaagagg cttaataaaa aatcctaatc aagtttatta ggacaacaaa
    13681 tattgtacaa aaattaatcc cccgagctat aatgcttggg ggatttttct ataggagggc
    13741 tagtttagtt ctctttttta ttttttagta agaaaccgag accaaggagc gcaaggaggc
    13801 tgattcctgc aaataggagt ttgttttcgt ttttgattcc tgtatttgga agttcagctt
    13861 gtttatctgg ggttgcaggt atattttcct tgctagagtt ttctaccgat tcgtttcgaa
    13921 ccgcttcttt tacagatgat gcttgtgctt cttgtgctgg ttgttcagct tgtgaatcag
    13981 ctgtagcttg gctaggttta ttttccgcat cagctacttt ggcatctggt ttagtagaat
    14041 caggtctagc ttctggagct gggtcttgat gttcaggctt ggaaggggtt gagtctggtt
    14101 tctcttctgc agttgactca tggctagttc cattttgaga gactcgaaga acagtagctg
    14161 taagggcatt caatttcaag cctttttcag tccattcaag tcctttcggg ttggcaattc
    14221 cgactgatcc tgcttggttt tcatctgcca aaacttccgc atttcttaga tgtgcaaagg
    14281 cagttcccaa attaaattcg cgagcttttt catccgcatt gacaaagact gcgtagatat
    14341 cgccgtttgg agcagtgatt tggtagccaa tcactacatc ctctttttcc acaccatttt
    14401 ggcctgggac agtgatgagg tggacacggt ctttgatatc ttgaagactc ttaagtcgga
    14461 aggcatctgt agattgacga agggcaatca aacctttcat atagtcacgg ctcttgacat
    14521 tttcaggata agctttacca tctgtagcct tagtccagtc aaacttgttg actgcgtcgc
    14581 tagaatcgta agaatcatgg atgaagtaag gatagtcaaa tgggttgccg tccttatcac
    14641 gcaacaagtg agatttgttt ggaaccttat cctctgctac tggagtcttg taggctgggt
    14701 cacggaattg tttagtacgt ccatattcct gaccggagtg gataaatgga gttccttgag
    14761 ctgtcaagac catgagattt ccaagtcgta aacgacggtg gatttcagca tagttctcag
    14821 ccttgcttgg gtcttttttg atagactggg caatgatgtc aaagagggtc aagttatcat
    14881 gggctgcgat gtattggatg acatctccag ggctgtcagc ttcaaagtta gttggctgag
    14941 caatgagatt tttaaagatg gtgttgacat cacgcttgcc acctgtgata aaggcaggtt
    15001 gaccttcgtt tggataacca gatttgaggt tgttacggat gtcatctgaa aagacagcga
    15061 cagtatcggt atgtttcatc caatcttggt cagcagcttt agtaggcatg ttttcatcac
    15121 cggcataggt tctccaacct tcaccaagca tgatgaggtt tggattgagg gcgcgtgcag
    15181 ctttgtaagc ttcttcgata gaagcggtgt catggtctcc catcatatcg aaacggaatc
    15241 catccacttt gtaggtatca actagatatt tgatagagtc aactaggagg cgtttggtca
    15301 tatggtgggt tgttcccaag cgtccaccac caaagctagt tcgaggtgta ccatcggcat
    15361 ccataaagtg gtagtagttt ggctctaggt cttcaaagat atcgactttg gctgtgtggt
    15421 tataaacgac atctaggata gctcccatac cacgtttgtg gatttcgttg atgaggtttt
    15481 taaattctgc gattcgtttt tctggattct taggatcgct tgagtacata ccagtcaagg
    15541 agaagtagtt ttgagggtca tatccccagt tgtagttgct gttgcttgaa gcatagtcag
    15601 acaagcgttc atggtttttc aattcattga caaagtagta agacaagact ggaaggagct
    15661 ggatatgggt tacacccaag tctttgagat agtctagttt ttcaatgaag gcttcaaaag
    15721 tcccaaatgg tttggtcaag tcttttgcaa tggcaggatc tgaagtgaag tcacgcacat
    15781 gagcttcgta gataacggcg tcttcacgag tcttgaaatt gtgaatctta ccataagtca
    15841 agtcttgagg tccgagttta gctggatcta caaaggcggc tttagccact ttatgggcat
    15901 cgtcaatctt ggcatcgtcg ctattccaag cagcaagaga tttagcgtaa ggatcgagtg
    15961 caagaacagt tttaccttga cgctcgattt ggtattgata atagtagcca gtgaaatctg
    16021 tgattccgag tttgtttgtg ctgtctagag tttgtttcca agttcctctg tccccttttt
    16081 cgagagcgac agttccaact actttgtcag ggtcattctt gtcgtagaca acaacagaaa
    16141 ccttatcagc acttggtgac caaagggtca aatcaacttg ttttccttct tcttttaggt
    16201 cagctcccag tttgccatca tagctgtatg tctcatcttt caggcgccag cttgttttgg
    16261 tagtgaattg gtcggaattg tagctaacag tataaggatg ttttgtgtca gagaaatctc
    16321 cgctgtaggt cactttctta ccagcttcat cgattgcaac atcggtaata gttaccttgt
    16381 ttcctaggtg attagtgatg ttggagtgtt tgaggatatc ttctttttta gcaccgacaa
    16441 gtgttgaaaa gctactttca atgctagaag tgcctacgtg ttgggctcct gtcatacgga
    16501 tatcatggac atagtatgga tttgtgtaaa tcgattcatc atcgtctttt aggaaaattt
    16561 ggctatgatt tttcaaatct gtgaacttat aattttcttt acggattttc acgtcgtctc
    16621 cttgtttgct ctcatctagt aataaaaatc caaattctct tgcggcttca ttaagaggaa
    16681 tgtcgatata gcggccatat ttgcctgtag ccgtaaagtc tgttccgtca ggccattgag
    16741 cgctacttgg attttttaca tctccccagt accagagaga tttcttgtca tagttgccat
    16801 ctgtgcggta gtagttgacg cgaacagttc ctgcaggttg tggctcgtaa gagaaaacct
    16861 tgtaatcttg gtctaaccaa gcctcgttca tttttggaac tagtttttct acagatttat
    16921 cgccggttag attttttcca gctgtattgt tgatgaggaa gctaattttc ttggcttgtt
    16981 ctccctttaa tttgacatct aggtaatagc cgtagtcatc tttcttggca tccttgaagg
    17041 acaaagctcc gtttggccag ttttcagatg gtttttcaac atcgtcccaa gtccatagtc
    17101 cttgagcatc cttgttttct tcaggaagtt ttttgacatg gatacggaag tagttatctt
    17161 cgatgggctc ttctgcctta gtttcagttg ttactggact agtagaagta gttggctctg
    17221 ttgcaggttt ttcttctgtt ttctctaggg aagcgtttgc atttctaggt gaagaaatat
    17281 cactttcgtt cttaccagag ccagcagctg tatcagtaag aggttgggca agggtggttg
    17341 tatttctgta gttgggttcg ttagtggttg gtgtactttc gtcggatgag atagttggtg
    17401 tagccatggc aagcaagaca aggcttgcgc cgataaggac agaaccagtt ccatttttga
    17461 gggaacggat gctgtagacc atttttttct cagtgtgaga tggtgttttt ctcataatga
    17521 ttctccaatc aataaatttc tatatcagta tagcatgtag taaaacaata tgcaagcgtt
    17581 ttcttttatt tgtgaaaaaa attcttactt ttgagaagtt ttctgtacta ttttatatgt
    17641 cagtataaat gacggagata aaaatgtcac ataagccaag ccgtattatt aggaagatga
    17701 tgtgttccca gctattttta ttcagaaatt gatctctaac attaccaata aaaaagaaaa
    17761 atatcttgac aagcaaggga aaattttgtt acaataatag acggtacttt ttacttttgg
    17821 tctctcaaga gtgtacaggg acgtgctgac aaatgttgca aaagtacaca cagatgatat
    17881 ctgtcaccaa gtgtatcatc accaaaaata aaaaaacaca ggagaatgta gatgcctaca
    17941 attaaccaat tggttcgcaa accgcgtaaa tcaaaagtag aaaaatctaa atcaccagct
    18001 ttgaacgttg gttacaatag tcataaaaaa gttcaaacaa acgtttcttc accacaaaaa
    18061 cgtggtgttg caactcgtgt tggaacaatg acacctaaaa aacctaactc agcccttcgt
    18121 aaattcgctc gtgtacgttt gagcaacctt atcgaagtta ctgcctacat cccaggtatc
    18181 ggacacaact tgcaagagca cagcgtggtg cttcttcgcg gtggacgtgt aaaagacctt
    18241 ccaggggtac gttaccatat cgtccgtggt gcacttgata ctgcaggtgt taacgatcgt
    18301 aaacaaggcc gttctaaata cggtactaaa cgtccaaaag cataaggaaa ggggataaag
    18361 agaaatgagt cgtaaaaata gagctccaaa acgtgacgta ttgccagatc cgctatacaa
    18421 ttcacaacta gttactcgtc ttatcaaccg cgttatgctt gatggtaaac gtggtactgc
    18481 tgcttcaatc gtttacggtg cttttgagca aatcaaagaa gctactggca acgatgcact
    18541 tgaagtattt gaaacagcta tggaaaacat catgcctgta cttgaagtac gtgcacgtcg
    18601 tgttggtggt tctaactacc aagtcccagt tgaagttcgt ccagaacgtc gtacaacact
    18661 tggacttcgt tggttggtaa caatcgctcg tcttcgtggt gaacacacaa tgcaagaccg
    18721 tcttgcaaaa gaaatcttgg atgctgctaa caacactggt gcagctgtta agaaacgtga
    18781 agatactcac cgtatggctg aagctaaccg tgcattcgca cacttccgtt ggtaagatag
    18841 gatgcgaaag cgttaagaaa gtcccagaga aaatagggaa tcgaagcagg ttgcggttgc
    18901 aaccaatgag attcatcttt ttctccagac ttttagcttg agctcaacta aatcatgatg
    18961 ctaggcacgg taaggatgca aggtgaaaat aggaaactga cgcagtattc aacgaataca
    19021 aggagtttta tctttttcac gcagcatccc gttccagctc acatcggcta actaacttta
    19081 gcccgggttc aaattagcta aatcgattag tattagctat aactcagctt accatctcgt
    19141 aagttgaaac caacaatagc atgaaaacat tgagaacggg taggtcctgc ctatccgttt
    19201 ttattaaaat cgtgttataa tagaatagaa atcaaaaata aataggagaa acaaacctca
    19261 tggcacgcga attttcactt gaaaaaactc gtaatatcgg tatcatggct cacgtcgatg
    19321 ccggtaaaac aacaactact gagcgtattc tttactacac tggtaaaatc cacaaaatcg
    19381 gtgaaactca cgaaggtgcg tcacaaatgg actggatgga gcaagagcaa gaacgtggta
    19441 tcacgatcac atctgctgcg acaacagctc aatggaacaa ccaccgcgta aacatcatcg
    19501 acacaccagg acacgtggac ttcacaatcg aagtacaacg ttctcttcgt gtattggatg
    19561 gtgcggttac cgttcttgac tcacaatcag gtgttgaacc tcaaactgaa acagtttggc
    19621 gtcaagcaac tgagtacgga gttccacgta tcgtatttgc caacaaaatg gacaaaatcg
    19681 gtgctgactt cctttactct gtaagcacac ttcacgatcg tcttcaagca aatgcacacc
    19741 caatccaatt gccaatcggt tctgaagatg acttccgtgg tatcattgac ttgatcaaga
    19801 tgaaagctga aatctatact aacgaccttg gtacggatat ccttgaagaa gacatcccag
    19861 ctgaatacct tgaccaagct caagaatacc gtgaaaaatt gattgaagca gttgctgaaa
    19921 ctgacgaaga attgatgatg aaatacctcg aaggtgaaga aatcactaac gaagaattga
    19981 aagctggtat ccgtaaagcg actatcaacg ttgaattctt cccagtattg tgtggttcag
    20041 ccttcaaaaa caaaggtgtt caattgatgc ttgatgcggt tatcgactac cttccaagtc
    20101 cacttgacat cccagcaatc aaaggtatta acccagatac agacgctgaa gaaactcgtc
    20161 cagcatctga cgaagagcca tttgcagctc ttgccttcaa gatcatgact gacccattcg
    20221 taggtcgttt gacattcttc cgtgtttact caggtgttct tcaatcaggt tcatacgtat
    20281 tgaatacttc taaaggtaaa cgtgaacgta tcggacgtat ccttcaaatg cacgctaaca
    20341 gccgtcaaga aatcgacact gtttactcag gtgatatcgc tgctgccgtt ggtttgaaag
    20401 atactacaac tggtgactca ttgacagatg aaaaatctaa aatcatcctt gagtcaatca
    20461 acgttccaga accagttatc caattgatgg ttgagccaaa atctaaagct gaccaagaca
    20521 agatgggtat cgcccttcaa aaattggctg aagaagatcc aacattccgc gttgaaacaa
    20581 acgttgaaac tggtgaaaca gttatctcag gtatgggtga acttcacctt gacgtccttg
    20641 ttgatcgtat gcgtcgtgag ttcaaagttg aagcgaacgt aggtgcgcct caagtatctt
    20701 accgtgaaac attccgcgct tctactcaag cacgtggatt cttcaaacgt cagtctggtg
    20761 gtaaaggtca attcggtgat gtgtggattg aatttactcc aaacgaagaa ggtaaaggat
    20821 tcgaattcga aaacgcaatc gtcggtggtg tggttcctcg tgaatttatc ccagcggttg
    20881 aaaaaggttt ggtagaatct atggctaacg gtgttcttgc aggttaccca atggttgacg
    20941 ttaaagctaa gctttatgat ggttcatatc acgatgtcga ctcatctgaa actgccttca
    21001 agattgcggc ttcactttcc cttaaagaag ctgctaaatc agcacaacca gctatccttg
    21061 aaccaatgat gcttgtaaca atcactgttc cagaagaaaa ccttggtgat gttatgggtc
    21121 acgtaactgc tcgtcgtgga cgtgtagatg gtatggaagc acacggtaac agccaaatcg
    21181 ttcgtgctta cgttccactt gctgaaatgt tcggttacgc aacagttctt cgttctgcat
    21241 ctcaaggacg tggtacattc atgatggtat ttgaccacta cgaagatgta cctaagtcag
    21301 tacaagaaga aattattaag aaaaataaag gtgaagacta atccgtcctc actctagaag
    21361 gaagtcactt agtggcttcc ttttgtcttt agaaaatacc tctaaatatg gtaaaatagt
    21421 agaagaataa tgtgaggaaa atgaatgtca aatagttttg aaattttgat gaatcaattg
    21481 gggatgcctg ctgaaatgag acaggctcct gctttagcac aggccaatat tgagcgagtt
    21541 gtggttcata aaattagtaa ggtatgggag tttcatttcg tattttctaa tattttaccg
    21601 attgaaatct ttttagaatt aaagaaaggt ttgagcgaag aattttctaa gacaggcaat
    21661 aaagctgttt ttgaaattaa ggctcggtct caagaatttt caaatcagct cttgcagtcc
    21721 tactataggg aggctttctc tgaaggtcca tgtgctagtc aaggttttaa gtccctttat
    21781 caaaatttgc aagttcgtgc tgagggtaat cagctattta ttgaaggatc tgaagcgatt
    21841 gataaggaac attttaagaa gaatcatctt cctaatttag ccaaacaact tgaaaagttt
    21901 ggttttccaa cttttaactg tcaagtcgag aagaatgatg tcctgaccca agagcaggaa
    21961 gaggcctttc atgctgaaaa tgagcagatt gttcaagctg ccaatgagga agcgcttcgt
    22021 gctatggaac aactggagca gatggcacct cctccagcgg aagagaaacc agtctttgat
    22081 tttcaagcga aaaaagctgc agctaaaccc aagctggata aggcggagat tactcctatg
    22141 atcgaagtga cgacagagga aaatcgtctg gtatttgaag gggttgtttt tgatgtggag
    22201 caaaaagtga ctagaacagg tcgtgtttta atcaacttta aaatgacgga ctatacttca
    22261 agtttttcta tgcaaaagtg ggttaaaaac gaggaagagg cccagaagtt tgacctcatc
    22321 aagaagaatt cttggctccg agttcgaggg aatgtggaga tgaataactt cacacgcgat
    22381 ttgactatga acgtacagga tctgcaggaa gttgttcact atgagcggaa ggatttgatg
    22441 ccagaaggtg agcgtcgggt tgagtttcat gctcatacta acatgtcgac tatggatgct
    22501 ttgccagagg tcgaagagat tgttgcaaca gctgctaagt ggggacacaa ggcggttgct
    22561 atcacagacc atgggaatgt ccagtccttt ccacatggct ataaggcggc taagaaagcg
    22621 ggaatccagc tgatctatgg gatggaagcc aatatcgtgg aggaccgtgt ccctatcgtc
    22681 tataacgaag tggagatgga cttatcagaa gcaatctacg tggtctttga cgtggaaacg
    22741 acgggacttt cagctatcta taatgacttg attcaggttg cggcctctaa gatgtacaag
    22801 gggaatgtta ttgctgaatt tgatgaattt atcaatcctg ggcatccctt gtcagctttt
    22861 actacagagt taactggaat tacagatgat catgtcaaaa atgccaaacc actagaacaa
    22921 gttttgcaag aattccaaga attttgcaag gatacggtcc tagttgccca caatgctacc
    22981 tttgacgttg gctttatgaa tgctaattat gagcgtcatg atcttccaaa gattagtcag
    23041 ccagttattg atacgctgga gtttgctaga aacctctatc ctgagtataa acgccatggt
    23101 ttggggcctt tgaccaagcg ttttggtgtg gccttggaac atcaccacat ggccaactac
    23161 gatgcggaag cgactggtcg tctgcttttc atctttatca aagaggtagc agaaaaacat
    23221 ggtgtgaccg atttagctag actcaacatt gatctaatca gtccagattc ttacaaaaaa
    23281 gctcggatca agcatgcgac catctatgtc aagaatcagg taggtctaaa aaatatcttt
    23341 aagctggttt ccttgtctaa taccaagtat tttgaaggag tgccacggat tccgagaacg
    23401 gttctagatg cccatcgaga gggcttgatt ttaggttcag cctgttcaga gggtgaagtt
    23461 tttgacgtgg tcgtttctca aggtgtggat gcggcggttg aggtggccaa gtattatgac
    23521 tttatcgagg tcatgccacc ggctatctat gcgcccttga ttgctaaaga gcaggtcaag
    23581 gatatggagg aactccagac cattatcaag agtttgatag aggttggaga ccgccttggc
    23641 aagcctgttc tggctacggg aaatgttcac tatatcgaac cggaagaaga gatttatcgt
    23701 gaaattatcg tccgtagttt gggacagggt gcgatgatta accgaactat cggtcatggt
    23761 gaacatgccc aaccagcgcc acttccaaag gctcattttc gaacgactaa tgagatgttg
    23821 gatgaatttg cctttttggg agaggaactg gctcgtaaac tggttattga aaacactaat
    23881 gccttggcag aaatatttga acccgttgaa gtcgttaagg gtgacttgta tacgcctttc
    23941 atcaacaagg ctgaagaaac agttgctgag ttgacctata agaaagcttt tgagatttat
    24001 ggaaatccgc tgccagatat tgttgatttg cggattgaaa aagaattaac atccatactg
    24061 gggaatggat ttgctgtgat ttatctggca tcgcagatgc tggtgcaacg ttctaatgaa
    24121 cggggttatt tggttggttc tcgtgggtct gtcggatcta gtttcgttgc gaccatgatt
    24181 gggattacgg aggtcaatcc tctctctcct cactatgtct gtggtcagtg tcagtacagt
    24241 gagtttatca cagatggttc gtacggttca ggatttgata tgccccataa ggactgtcca
    24301 aactgtggtc acaaactcag taaaaacgga caggatattc cgtttgagac cttccttggt
    24361 tttgatgggg ataaggttcc tgatattgac ttgaacttct cgggagaaga tcagcctagc
    24421 gcccacttgg atgtgcgtga tatctttggt gaagaatatg ccttccgtgc gggaacggtt
    24481 ggtacggtag ctgccaagac tgcctatgga tttgtcaagg gttacgagcg agattatggc
    24541 aagttttatc gtgatgcaga agtagaacgc ctcgctcaag gagcggcggg tgtcaagcgg
    24601 acaacaggcc aacacccggg gggaatcgtt gttattccga actacatgga tgtctacgat
    24661 tttacgcctg tccagtatcc agcagatgat gtcacggctg aatggcagac cactcacttt
    24721 aacttccacg atatcgatga gaacgtcctc aaactcgatg tactgggaca tgatgatccg
    24781 accatgattc gaaaacttca ggatttgtct ggtattgacc ctaataaaat tcctatggat
    24841 gacgaaggcg tgatggcact cttttctggg actgatgtgc taggggtaac acctgaacaa
    24901 attggaacgc ctacgggtat gttggggatt ccagagtttg gaacaaattt cgtacgtgga
    24961 atggtagacg aaacccatcc gacaaccttt gcggaattgc ttcagctgtc tggtctgtcc
    25021 cacggtactg acgtttggtt ggggaatgct caggatctga ttaagcaagg aatagcggac
    25081 ctatcgactg ttatcggttg tcgggacgac atcatggttt acctcatgca tgcgggtctg
    25141 gaacctaaga tggcctttac cattatggaa cgggtacgta agggtttgtg gctaaagatt
    25201 tcagaagagg agagaaatgg ctatatcgaa gccatgaagg ctaataaggt gccagagtgg
    25261 tatatcgaat cctgtgggaa aattaagtac atgttcccta aggcccatgc ggcagcctac
    25321 gttatgatgg ccttgcgtgt agcttacttc aaggttcacc atcctattta ttactactgt
    25381 gcttacttct ccattcgtgc taaggctttt gatatcaaga ccatgggtgc gggcttggag
    25441 gccatcaagc gcagaatgga agaaatctct gaaaaacgga agaacaatga agcctctaat
    25501 gtggaaatcg atctctatac aactcttgag attgtcaatg agatgtggga acgaggtttc
    25561 aagtttggca aattagatct ctaccgtagt caggcgacag agttcctcat cgacggggat
    25621 acccttatcc caccatttgt agcaatggat ggtctgggag agaacgttgc caagcaactg
    25681 gtgcgggcgc gtgaagaggg agaattcctc tctaaaacag aactacgcaa gcgtggtgga
    25741 ctctcatcaa ccttggttga aaagatggat gagatgggta ttcttggaaa tatgccagag
    25801 gataaccagt tgagtttgtt tgatgagttg ttttaaaaaa ttgcttaata atctattaaa
    25861 agaggctaac gtatatccaa tagatttaca ttagctttct tttttgttaa aatagtctat
    25921 ggaaagaggg tgagagtatg tcaaagatga gtataagcat ccgtctggat agtgaggtta
    25981 aggagcaggc ccaacaggtg tttagtaatc tgggaatgga tatgacaaca gctattaata
    26041 ttttccttcg tcaggcaatt caatatcagg gattaccttt tgatgttaga ctagacgaaa
    26101 atcggaagtt gctccaagtg ttaacggatt tagaccaaaa tcgtaatatg agccagtctt
    26161 ttgaatcagt ctcagatttg atggaggact tacgtgctta agattcgtta tcataaacag
    26221 tttaaaaaag attttaagtt ggctatgaag cgtggtttga aggcagaatt attagaagaa
    26281 gttttgaatt ttctggttca agaaaaagaa catcctgcca gatatcgtga tcattcattg
    26341 acggcatcca agcattttca aggagttcgt gaatgccata cccagccaga ttggtttttg
    26401 gtttataaag tagacaagtc ggaattgatt ttaaatttgc tgaggacagg cagtcacagt
    26461 gatttatttt aatctatttt aagggggttc tcatgaaact aagaatattt gcggaagata
    26521 agccggctaa gaaggtattt gaatatcaat tagaacttgc tgatcgtaca attcttctat
    26581 cgacagcact cttgtcaggt gctattgctt tagcaggaat cttttctgct ttgagagaaa
    26641 aataaaaata gaaaagagaa aacaggatgg ttttaccaaa ttttaaagaa aatctagaaa
    26701 aatatgcgaa attgttggtt gcaaatggaa tcaacgtgca acctggtcac actttggctc
    26761 tctctatcga tgtggagcaa cgtgagttgg ctcacttaat cgtcaaagaa gcttatgctc
    26821 tgggtgcgca tgaggttatc gttcagtgga cagatgatgt cattaaccgt gagaaattcc
    26881 tctatgcgcc aatggagcgt ttggacaatg tgccagaata caagattgct gagatgaact
    26941 atcttttgga gaacaaggct agccgtcttg gggttcgttc atctgatcca ggtgccttga
    27001 acggagtgga tgctgacaag ctttcagctt ctgctaaagc tatgggactt gccatgaagc
    27061 ctatgcgtat cgcaactcaa tctaacaaag ttagctggac tgtagcagct gcagcaggac
    27121 ttgagtgggc taagaaagtc ttcccaaatg ctgcgagcga cgaagaagca gttgatttcc
    27181 tttgggacca aattttcaaa acttgccgtg tctacgaagc agatcctgtt aaggcttggg
    27241 aggaacatgc agccattctc aagagcaagg ccgatatgct taataaggag caattttcag
    27301 cccttcacta cacagcgcca ggaacagatt taacacttgg tttgccaaag aaccacgttt
    27361 gggaatcagc tggtgctgtc aatgcacagg gcgaagaatt cttgccaaat atgccgacag
    27421 aagaggtctt cacagcgcct gacttccgtc gtgcagatgg ttatgtcact tctacaaaac
    27481 cgcttagcta caacggaaat atcattgaag gcattaaggt gacctttaag gatggacaaa
    27541 tcgtagatat cactgctgag aagggtgatc aggttatgaa agaccttgtc tttgaaaatg
    27601 cgggtgcgcg tgccttgggt gaatgtgcct tggtaccaga tccaagtcca atttctcagt
    27661 caggcattac cttctttaac acccttttcg atgaaaatgc gtcaaaccac ttggctatcg
    27721 gtgcagccta tgcgactagc gtcgttggtg gagcagagat gagtgaagaa gagcttgaag
    27781 ctgcagggct taaccgttca gatgttcacg tagactttat gattggttct aaccaaatgg
    27841 atatcgatgg tattcgtgag gatggaacgc gagtacctct tttccgtaat gggaattggg
    27901 caaattaagg agataatatg ttaggaagta tgttcgttgg tctcctagtg ggatttttag
    27961 caggtgctat gaccaatcgt ggagagcgaa tgggatgttt tggaaaaatg tttctcggtt
    28021 ggatcggagc ctttctaggt cacttgctct ttggaacttg ggggccagtt ttatcaggaa
    28081 cagctattat cccagctgtt ttaggtgcca tgattgtctt agctattttt tggagacgag
    28141 gaagttaatt tcttaaatct gatactcaat gaaaatcaaa gagcaaacta ggaaactagc
    28201 cgcaggttgc tcaaagtact gctttgaggt tgcagataga actgacgaag tcagtaacat
    28261 atatacggca aggcgacgtt gacgcggttt gaagagattt ttgaagagta tgaaacgaaa
    28321 aggaggttgg tcattgtacc agcctccttt tgatatgata taatagttct atgagattag
    28381 ataaattttt agttgcctgt gcggtgggaa gtcggactga ggtcaaaaac ttgctcaagg
    28441 ctgggcgcgt gactgtaaat ggtaaaaaag aaaaatcagc taaattgcag attgatgaaa
    28501 aaatagatga gattcgcttt gatgggcaag tgttggagta tgaagagttt gtctactaca
    28561 tgatgaacaa gcccaaagga gttatatcag cgactgagga tcccaagcac agaaccgttc
    28621 tggacttgtt ggatgacttg gcgcggagta aggaaatttt cccagtagga cgcttggata
    28681 ttgacacgca tggtcttttg ctcttgacca atgatggtca gctggctcat gttcttcttt
    28741 cgcccaagcg tcatgtggac aagacttatc tggcacaagt caagggaatc atgacccaag
    28801 aagatgtgga gacatttgct gagggtattc ctctcaaaga ctttacctgt caacccgcta
    28861 tactggagct tgtatccata gatacagaaa agaatcaaag ccaaatccgt gtgaccattg
    28921 cagaagggaa gtttcatcag atcaagcgta tggtgggcta ctgtggcaag gaagtggtgg
    28981 acttgcaacg attgactatg ggaactttag tattagatga gaacctagaa cgaggggaat
    29041 ggcgtcgctt gaccaaggaa gaattagaaa ttctccgcgc taatattatt taatttagtt
    29101 taatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg tacttgagta
    29161 cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtataaat tcgaaaaagg
    29221 tgagggacaa tgtcctcgcc ttttatgttt tttagttgct tcctttgtga aaagagttat
    29281 aatagactgt agaataaaag gaggaatcta tgaacgcgat tcaagaatca tttactgata
    29341 aactatttgc caactatgaa gcaaatgtaa aataccaagc gattgaaaat gctgccagcc
    29401 acaacggaat ttttgcagct ctggaacgtc gccaaagcca tgtagacaac acacctgttt
    29461 tctcattgga tttaaccaag gacaaggtca ctaaccagaa agcgtctggt cgttgctgga
    29521 tgtttgcggc tctcaacacc ttccgccaca aactcatctc gcaatacaaa ttggagaact
    29581 ttgagttgtc acaagcccac actttcttct gggataagta tgagaaatca aactggttct
    29641 tggagcaagt cattgcgact tcagaccaag aattgactag ccgcaaggtt agcttcttac
    29701 tccaaacacc tcaacaagat ggcggtcaat gggatatggt cgtttccctc tttgaaaaat
    29761 acggtgtcgt gcctaagtca gtttatcctg agtctgtttc atctagcagc agtcgtgagc
    29821 taaatgcgat ccttaataaa ttgcttcgtc aagatgctca aatcttgcgt gacttgcttg
    29881 tttctggtgc agatcaagcg actgttcaag ctaagaaaga agacctcttg caagaaatct
    29941 ttaactttct tgctatgtca ttgggacttc caccacgcaa gtttgacttt gcttatcgcg
    30001 ataaagataa caactacaaa agtgaaaaag gaatcacacc acaagagttt tacaagaaat
    30061 atgtcaatct tcctttagaa gactacgttt ctgttatcaa tgctccaact gctgataaac
    30121 cttacggaaa atcttacaca gttgagatgt tggggaatgt tgttggtagc cgtgcagttc
    30181 gctacatcaa cgttccaatg gagcgcttga aagaattggc gattgcccaa atgcaagcag
    30241 gtgagactgt ttggtttggt tctgatgtcg gccagctcag caaccgtaag gctggaatcc
    30301 ttgcgacaga tgtttatgac tttgaatcaa gcatggacat taaacttact caagacaagg
    30361 ctggacgttt ggactatagc gaaagcttga tgacccacgc catggtcttg acaggtgttg
    30421 acttggacga aaatggtaaa tcaaccaagt ggaaggttga aaactcatgg ggagacaagg
    30481 tcggtacaga tggttacttt gttgcctcag acgcttggat ggacgaatac acataccaaa
    30541 tcgttgttcg taaggaattg ctgacagcag aagaacaagc tgcctatgga gcagaaccaa
    30601 tcgtacttgc accatgggat ccaatgggag ccttggctga ataaaagcat agaaaaaagg
    30661 aatcagattt tagaacctgg ttccttttta gttgcttgat tacatgatgt gaagaacatg
    30721 tgccacaata cccactgcga agagggcaag gataatagtg attggagata cttttttctt
    30781 aagtaagtac atgcaaagta aagtaaggag tagtcctgat aatccaggaa tcaacatatc
    30841 caagttttgt tggaaagtag taactttttc aggagtttga gacaatcctt gtcctacttg
    30901 tgcgaatgct tcttggatac ctttagaccc ttctggcaat ttatcccaat ggatataagc
    30961 cttttcatct agttgaactt tagaaacatc gaaagcaaat ttaatattta cccaacgttg
    31021 aacaaggaca gcaagaatga acatcccaag gatagaagct cctttagtga tatcttgaag
    31081 gataccacca gacatatctt tagtgatttc tgatccagcc ttgtatccaa tctcttgaac
    31141 ataccacaag aatgacatac gaatcaagtt ccatgcaaca aagaagagga gtggacccaa
    31201 gatattgcca gtaagggcaa gtgaagcacc gagagatcca aggattgggc gtactgtaaa
    31261 ccagaatact gggtcaccga taccagcaag aggtcccatc ataccgattt tgaccccttg
    31321 gatagcagcg tcatcgattt ccacaccgtt agcacgttct tcttcaagcg caagagtaac
    31381 ccccatgact ggagcagcta cgtatggatg agtgttgaag aactcaaggt gacgctcaag
    31441 agcagcgatt tgatcttctt ttttagtata gagttttttg atagctggaa tgagtgtata
    31501 agcccagccc aagttttgca tccgttcaaa gttccaagac ccttgtaaga aggttgaacg
    31561 ccaccaaact tttttacgat ctgatttagt taattgaagt ttttcagtca tgatgttttc
    31621 agtcctttct tatcttagta gtcttctagg atatcgccga ttgggtcgtt agaagttgcg
    31681 gctcctccgc caccatttcc accagtttta gaaaggtgaa ggtagataag agcgatagca
    31741 acaccgatag caccgaatcc gattagagta atatctgaca cagcagcgag aacgaaacca
    31801 agagcgaaga atggccatac ttcacgagtt gccatcatgt tgataaccat ggcgtaacca
    31861 acggcaacga ccataccacc accgatagcc ataccatctt tgagccagtc tggcatggca
    31921 ctaaggatac tttgtacagt ttcagttggt accataagga gaagagctgc aggaagcgcg
    31981 atacgaagtc cttggaaaag tagcgcaatg aaatgagcac gctccacagc gccgaagtca
    32041 ccttttttag cggcagcatc agcagtgtga accaatccaa ctgaaattgt acgaacaatc
    32101 atagttaaga atagaccagc tacggcaaga gggatagcaa ccgcttgggc aacaccgata
    32161 ccagtcttgg taaagtcacc accaagaacc atgataatgg cagcagcgac agaagcaagt
    32221 gcagcatcag gagcgatagc agcaccgata tttgaccaac caagggcaat catttgaagc
    32281 gatccaccga ggataatccc tgcttccaag tgacctgtta caagcccaat aagggtacag
    32341 gctacaagtg gttggtgaaa ttggaactgg tcgaggatgc cttcaagacc tgcaaagaag
    32401 gctacaacga ctactaaaac catagaaata atagacatgt ttaaaatcct ttcataaata
    32461 atggcttatt tgacattggc tttgttaatc aagtcaaaca aatctttttt agaatcattt
    32521 ggtactttac ggacatcaaa ttcaacaccc aagtcacgca ttttttcaaa tgtagcaaca
    32581 tcttctttgt ccatagacaa aacggtattg accaatgttt tacctgttga gtgagccata
    32641 gaaccaacgt taagagtctt gattggcacg ccgccttcga tggcacgaag ggcatcttga
    32701 ggtgtttcaa acaagataag ggcatgtgtt tctccaaaac gtgggtcttt tgaaatctca
    32761 atcagttttt gaattggaac cacgttagcc ttgacattac ctggagctgc ttgtttaatc
    32821 aattctttac gaaggtcgtc tttagccaca ttatctgaag caacgatgat acgatttgct
    32881 tttgaatctg gagtccaagc agttgcaacc tgaccgtgaa gtagacgtgt gtcaagacgg
    32941 gcaagattga ttttcaattt accgtctccg ataacagttc cttctgggat agcagtttgg
    33001 gcaactggag cagctgcagc gcttgcaact tcttcaactg gatttagctc ttctggaaga
    33061 gctttgatgc catctttggc ttctttaatg atattagcag cgactttttc tacacctgca
    33121 gcagcgtcca tgaggcgctc tgtgtaggct tgaatcaaca tcggtaagtt aagtcctgtg
    33181 atgatggcaa acttacgctc aggattttct cccatcacgc gactagcttg gttaaatgga
    33241 gaaccactcc aaaggtcagc caaaactaga acctcatctt ctgcgtcaaa tgcagcaaca
    33301 gcgttattaa acttagcgta tagatcatca ggaccttcat ttggcataaa ggtcacaact
    33361 tgaacctttt cttgttcacc aaagatcata gatcctgact gatgaatacc cgcagcaaat
    33421 tcgccgtggc tcgcaataat gattccgata ctcattattg tcattcctcc ttttttgttt
    33481 tagttttcat ttaagaaaac tttaagactg tttaagtata aaccgttttc ataaaaaaag
    33541 caactat
//
LOCUS       DAVKZB010000010        33010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKZB010000010 DAVKZB010000000
VERSION     DAVKZB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.010"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            376..2529
                     /locus_tag="VXA80_000439"
     CDS             376..2529
                     /locus_tag="VXA80_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0352986.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTFAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGDFYAHLVNS"
     gene            2542..3891
                     /locus_tag="VXA80_000440"
     CDS             2542..3891
                     /locus_tag="VXA80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV0352987.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4061..4768
                     /locus_tag="VXA80_000441"
     CDS             4061..4768
                     /locus_tag="VXA80_000441"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV0352988.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4970..8695
                     /locus_tag="VXA80_000442"
     CDS             4970..8695
                     /locus_tag="VXA80_000442"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV0352989.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLTLATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLGGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKK
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8788..10230
                     /locus_tag="VXA80_000443"
     CDS             8788..10230
                     /locus_tag="VXA80_000443"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV0352990.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FYDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10267..11289
                     /locus_tag="VXA80_000444"
     CDS             10267..11289
                     /locus_tag="VXA80_000444"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV0352991.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGIGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11286..11831
                     /gene="purN"
                     /locus_tag="VXA80_000445"
     CDS             11286..11831
                     /gene="purN"
                     /locus_tag="VXA80_000445"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV0352992.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEVALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11915..12424
                     /locus_tag="VXA80_000446"
     CDS             11915..12424
                     /locus_tag="VXA80_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0352993.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVRVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12449..13996
                     /gene="purH"
                     /locus_tag="VXA80_000447"
     CDS             12449..13996
                     /gene="purH"
                     /locus_tag="VXA80_000447"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV0352994.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14119..15381
                     /gene="purD"
                     /locus_tag="VXA80_000448"
     CDS             14119..15381
                     /gene="purD"
                     /locus_tag="VXA80_000448"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV0352995.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVKKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15784..16272
                     /gene="purE"
                     /locus_tag="VXA80_000449"
     CDS             15784..16272
                     /gene="purE"
                     /locus_tag="VXA80_000449"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV0352996.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTILPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16259..17350
                     /gene="purK"
                     /locus_tag="VXA80_000450"
     CDS             16259..17350
                     /gene="purK"
                     /locus_tag="VXA80_000450"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV0352997.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17360..17587
                     /locus_tag="VXA80_000451"
                     /pseudo
     CDS             17360..17587
                     /locus_tag="VXA80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
     gene            17650..18948
                     /gene="purB"
                     /locus_tag="VXA80_000452"
     CDS             17650..18948
                     /gene="purB"
                     /locus_tag="VXA80_000452"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV0352998.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19003..22941)
                     /gene="strH"
                     /locus_tag="VXA80_000453"
     CDS             complement(19003..22941)
                     /gene="strH"
                     /locus_tag="VXA80_000453"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0352999.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMDFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQEEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSRSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23254..23970)
                     /locus_tag="VXA80_000454"
     CDS             complement(23254..23970)
                     /locus_tag="VXA80_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0353000.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24318..26105
                     /locus_tag="VXA80_000455"
     CDS             24318..26105
                     /locus_tag="VXA80_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV0353001.1"
                     /translation="MTRFEIRDDFFLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26102..26578
                     /locus_tag="VXA80_000456"
     CDS             26102..26578
                     /locus_tag="VXA80_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0353002.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26606..27511
                     /locus_tag="VXA80_000457"
     CDS             26606..27511
                     /locus_tag="VXA80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0353003.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27498..28313
                     /locus_tag="VXA80_000458"
     CDS             27498..28313
                     /locus_tag="VXA80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0353004.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28320..28724
                     /locus_tag="VXA80_000459"
     CDS             28320..28724
                     /locus_tag="VXA80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353005.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29023..30189
                     /locus_tag="VXA80_000460"
     CDS             29023..30189
                     /locus_tag="VXA80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV0353006.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30305..31342
                     /locus_tag="VXA80_000461"
     CDS             30305..31342
                     /locus_tag="VXA80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV0353007.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31550..32404
                     /locus_tag="VXA80_000462"
     CDS             31550..32404
                     /locus_tag="VXA80_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0353008.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALATQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            32554..32811
                     /locus_tag="VXA80_000463"
     CDS             32554..32811
                     /locus_tag="VXA80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV0353009.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         9099 a         6459 c         7748 g         9704 t
ORIGIN      
        1 actaaaattt ttttctttac ttaataatga tatgaaatca tttcggtttt gttaacttgt
       61 aattcttata ttttctaggt agaacgtttg ttctgcctat ttttttatcc aaaaagtgca
      121 gttgggaggg agataggctc atttgggaag gaagtccagt ttttgtttag tgattggggt
      181 aagatagttg ttatcagatg agtttatact cttcgaaaat caaattcaaa ccacgtcaac
      241 gtcgccttgc cgtatatatg tgactgactt cgtcagtcct atctacaacc tcaaaacagt
      301 gttttgagca gcctgcggct agtttcctag tttgctcttt gattttcatt gagtattagg
      361 gaaaaggaga tgaatatgaa atttgggaaa cgtcactatc gtccgcaagt ggatcagatg
      421 gactgcggtg tagcttcatt agccatggtt tttggctact atggtagtta ttattttttg
      481 gctcacttgc gagaattggc taagacgacc atggatggga cgacggcttt gggcttggtc
      541 aaggtggcag aggagattgg ttttgagacg cgagccatta aggcggatat gacgcttttt
      601 gacttgccgg atttgacttt tccttttgtt gcccatgtgc ttaaggaagg gaaattgctc
      661 cactactatg tggtgactgg gcaggataag gatagcattc atattgccga tccagatccc
      721 ggggtgaagt tgactaaact gccacgtgag cgttttgagg aagaatggac aggagtgact
      781 ctttttatgg cacctagtcc agactataag cctcataagg aacaaaaaaa tggtctgctc
      841 tcttttatcc ctatattagt gaagcagcgt ggcttgattg ccaatatcgt tttggcaaca
      901 ctcttggtaa ccgtgattaa cattgtgggt tcttattatc tgcagtctat cattgatacc
      961 tatgtgccag atcagatgcg ttcgacacta gggattattt ctattgggct agtcatcgtc
     1021 tacatcctcc agcaaatctt gtcttacgct caggagtatc tcttgcttgt tttggggcaa
     1081 cgcttgtcga ttgacgtgat tttgtcctat atcaagcatg tttttcacct ccctatgtcc
     1141 ttctttgcga cacgcaggac aggggagatc gtgtctcgtt ttacagatgc taacagtatc
     1201 atcgatgcgc tggcttcgac catcctttcg attttcctag atgtgtcaac ggttgtcatt
     1261 atttcccttg ttctattttc acaaaatacc aatctctttt tcatgacttt attggcgctt
     1321 cctatctaca cagtgattat ctttgccttt atgaagccgt ttgaaaagat gaatcgggat
     1381 accatggaag ccaatgcggt tctgtcttct tctatcattg aggacatcaa cggtattgag
     1441 actatcaagt ccttgaccag tgaaagtcag cgttaccaaa aaattgacaa ggaatttgtg
     1501 gattatctga agaaatcctt tacctatagt cgagcagaga gtcagcaaaa ggctctgaaa
     1561 aaggttgccc atctcttgct taatgtcggc attctctgga tgggggctgt tctggtcatg
     1621 gatggcaaga tgagtttggg gcagttgatt acctataata ccttgctggt ttactttacc
     1681 aatcctttgg aaaatatcat caatctgcaa accaagcttc agacagcgca ggttgccaat
     1741 aaccgtctaa atgaagtgta tctagtagct tctgagtttg aggagaagaa aacagttgag
     1801 gatttgagct tgatgaaggg agatatgacc ttcaagcagg ttcattacaa gtatggctat
     1861 ggtcgagacg tcttgtcgga tatcaattta accgttcccc aagggtctaa ggtggctttt
     1921 gtggggattt cagggtcagg taagacgact tttgccaaga tgatggttaa tttttacgac
     1981 ccaagtcaag gggagattag tctgggtggt gtcaatctca atcagattga taaaaaagcc
     2041 ctgcgccagt acatcaacta tctgcctcaa cagccctatg tctttaacgg aacgattttg
     2101 gagaatcttc ttttgggagc caaggagggg acgacacagg aagatatctt acgggcggtc
     2161 gaattggcag agattcgaga ggatatcgag cgcatgccac tgaattatca gacagaattg
     2221 acttcggatg gggcagggat ttcaggtggt caacgtcaga gaatcgcttt ggcgcgtgct
     2281 ctcttgacag atgcgccggt cttgattttg gatgaggcga ctagcagttt ggatattttg
     2341 acagagaagc ggattgtcga taatctcatt gctttggaca agaccttgat tttcattgct
     2401 caccgcttga ctattgctga gcggacagag aaggtggttg tcttggatca gggcaagatt
     2461 gtcgaagaag gaaagcatgc tgatttgctt gcacagggag acttttacgc ccatttggtc
     2521 aatagctaga aagaggagag gatgaaacca gaatttttag aaagtgcgga gttttataat
     2581 cgtcgttacc ataatttttc cagtagtgtg attgtaccca tggcccttct gctcgtgttt
     2641 ttacttggct ttgcaactgt tgcagagaag gagatgagtt tgtccactag agctactgtc
     2701 gaacccagtc gtatccttgc aaatatccag tcaactagca acaatcgtat tcttgtcaat
     2761 catttggaag aaaataagct ggttaagaag ggggatcttt tggttcaata ccaagaaggg
     2821 gcagagggtg tccaagcgga gtcctatgcc agtcagttgg acatgctaaa ggatcaaaaa
     2881 aagcaattgg agtatctgca aaagagcctg caagaagggg agaaccactt tccagaggag
     2941 gataagtttg gctaccaagc cacctttcgc gactacatca gtcaagcagg cagtcttagg
     3001 gctagtacat cgcaacaaaa tgagaccatc gcgtcccaga atgcagcagc tagccaaacc
     3061 caagccgaaa tcggcaacct catcagtcaa acagaggcta aaattcgcga ttaccagaca
     3121 gctaagtcag ctattgaaac aggtacttcc ttggccggtc agaatctagc ctactctctt
     3181 taccagtcct acaagtctca gggcgaggaa aatccccaaa ctaaggttca ggcagttgca
     3241 caggttgaag cacagatttc tcagttagaa tctagtcttg ctacttaccg tgtccagtat
     3301 gcaggttcag gtacccagca agcctatgcg tcagggttaa gcagtcaatt ggaatccctt
     3361 aaatcccaac acttggcaaa ggttggtcag gaattgaccc ttctagccca gaaaatcttg
     3421 gaggcagagt caggtaagaa ggtacaggga aatcttttag acaaggggaa aattacggca
     3481 agtgaggatg gggtgcttca tcttaatcct gagaccagtg attctagcat ggttgcagaa
     3541 ggtgccctac tagcccaact ttatccatct ttggaaagag aagggaaagc caaactcaca
     3601 gcctatctaa gttcaaaaga tgtagcaaga atcaaggtcg gtgattctgt tcgctatact
     3661 acgactcatg atgccgggaa tcaacttttt ctagattcta ctattacaag tattgatgcg
     3721 acagctacta agactgagaa agggaatttc tttaaaatcg aggcggagac taatctaact
     3781 tcggagcagg ctgaaaaact taggtacggg gtggaaggcc gtctacagat gattacgggc
     3841 aagaaaagtt atctacgtta ttatttggat caatttttga acaaagagta atgttcgtgt
     3901 ttttagagtt aaataatttt taaactgtga gaaagattct tcttgcagtt ttttctttac
     3961 aatttttgaa aaacacctgc tatttattcg gttaaattct tgtgtttttt ggttttttgt
     4021 ggtaaaatgt gctcaagtaa tacgaaaggc gaactttaaa atgtcaaaac aattgatcta
     4081 ttcgggaaaa gctaaagata tctatacaac tgaggatgaa aatcttatta tttcaactta
     4141 caaggaccag gcgactgctt tcaatggtgt caagaaggag cagattgcag gtaagggagt
     4201 cttgaataat cagatttcat cttttatttt tgagaaatta aatgcggctg gtgtggcgac
     4261 tcactttgtg gagaaacttt cagacacgga acaactcaat aaaaaggtta agattattcc
     4321 tttggaagtc gtgctccgca actatactgc tggttccttt tcaaaacgtt ttggtgtgga
     4381 tgagggaatc gccttggaga ctccgattgt cgaattttac tacaaaaatg atgatttgga
     4441 tgatccattt atcaatgatg agcatgtgaa attcctacag attgcgggtg accagcagat
     4501 tgcctacttg aaggaagaaa cgcgtcgtat caatgaacta ttgaaagtct ggtttgctga
     4561 gattgggctt aaattgattg actttaagct agagttcggt tttgacaagg atggcaagat
     4621 tatcttggca gacgaatttt caccagataa ctgccgcttg tgggacgctg atggcaacca
     4681 catggataag gatgttttcc gtagaggatt gggagaacta accgacgttt atgagattgt
     4741 ttgggaaaag ttgcaggaat tgaaataatc tgtttgcaac ggaaaacctt cgtctctcaa
     4801 ctaaaaggac tcaggctgaa aaggtccccc agaccttttc actctgtaga gaactaggtg
     4861 aactaacaga tgtttacgaa attgtctggg aaaagttgca gggtttaaaa taacaacctc
     4921 aaggctgttt gggaatattg caagagctga aataaaggaa taagaattga tggataaacg
     4981 tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg ttagagagct
     5041 ccagcacaac ttgggactgt caagcttgaa aagtattcgt attgtgcaag tatatgatgt
     5101 atttgacttg gctgaggact tgtttgcacc tgcagagaag cacattttct ctgagcaggg
     5161 aaccgaccat gttttagatg aagtatctgt gcaggcggat cttgctaact atgctttctt
     5221 tgccattgaa agtctgccag ggcagtttga ccagcgtgca gcttcgtcac aggaagcctt
     5281 gcttttgttg ggaagttcga gtgacgtgac agtcaacaca gcccaacttt acttggtgaa
     5341 taaagatatt gatgcgactg agttggaagc tgtcaaaaac tacctgctca atccagttga
     5401 ttctcgtttc aaggatatca cgacagggat tgccaagcag gagttttcag agtcagacaa
     5461 gaccattcca aaattgactt tctttgaaag ctatacagca gaagactttg ctcgctacaa
     5521 ggccgagcaa gggatggcca tggaagtgga tgatttgctc tttatccaag actactttaa
     5581 gtcaatcggg cgcgtgccaa ctgagactga actcaaggtt ttggacactt actggtctga
     5641 ccactgccgt catacgactt ttgagacaga gttgaaacac atcgactttt cagcttctaa
     5701 atttcaaaag caattgcagt caacctatga caagtatatt gccatgcgcg aggagttagg
     5761 tcggtctgaa aaaccacaaa ccttgatgga tatggcgact attttcggtc gttatgagcg
     5821 tgctaatgga cgattggatg atatggaagt ctctgacgaa atcaatgcct gctcagttga
     5881 aattgaagtg gatgttgatg gtgtcaagga accttggctc ctcatgttta aaaatgaaac
     5941 ccacaaccat ccaacagaaa ttgagccatt tggtggagcg gctacctgta tcggtggagc
     6001 tattcgtgat ccgttgtcag gccgttccta tgtttaccaa gccatgcgta tttcaggtgc
     6061 tggtgatatt acagcaccga tttcggaaac tcgtgctggg aaattgccac aacaagtcat
     6121 ttctaaaaca gcagctcatg gttattcttc atatggtaac cagattgggc ttgccacaac
     6181 ctacgttcgt gaatacttcc acccaggctt tgtagctaaa cgtatggaac ttggtgccgt
     6241 tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag gtgatgtgat
     6301 catccttctc ggaggcaaaa caggtcgtga tggtgtcggt ggtgcgacgg gctcttctaa
     6361 ggttcaaaca gttgagtctg tagagactgc tggtgctgag gttcaaaaag gaaatgccat
     6421 cgaagaacgc aagattcagc gcctcttccg taatggcaat gtcactcgtc tgatcaagaa
     6481 gtccaatgac tttggggcag gcggcgtctg tgtggctatc ggtgaattgg cagacggtct
     6541 tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc ttgaatggta cagaaattgc
     6601 catctctgaa tcacaagaac ggatggcggt cgtggttcgt cctgaagatg tggatgcctt
     6661 cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg gtggcgacag taactgaaaa
     6721 accaaatctt gtcatgcact ggaatggtga gacaatcgtt gacttggagc gtcgtttcct
     6781 tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg atgtcaaact
     6841 cccagaagag cgtcaaacat ctgctgaaac actggaatca gataccctta cggttctatc
     6901 tgacctcaac catgcaagtc aaaaaggatt acagactgtc tttgactgct ctgttggacg
     6961 ctcaacggtt aatcacccac ttggtggtcg ttaccaactc acaccaactg aggcatctgt
     7021 gcagaaattg ccagttcaac acggtgtgac tcatactgcg tcggtcattg ctcaaggttt
     7081 caacccatat gtagctgaat ggtctccata ccacggtgct gcttatgcgg ttatcgaagc
     7141 aactgctcgt ttggtggctg ctggtgccaa ctggtccaag gctcgtttct cttaccaaga
     7201 gtatttcgag cgtatggata aacaagcaga gcgtttcggt cagccagtag ctgctcttct
     7261 aggttctatt gaagcacaaa ttcagcttgg cttgccatct atcggtggta aggactccat
     7321 gtctggtacc tttgaagaat tgaccgttcc gccaaccttg gttgcctttg gggtgacgac
     7381 ggcagatagc cgtaaggtgc tctctccaga atttaaagct gttggggaaa atatctacta
     7441 catcccaggt caagccctct ctgcagagat tgattttgac ttgattaaga aaaattttac
     7501 tcagtttgaa gccatccaag ctgaccataa agtgacatct gcatcagctg tcaaatacgg
     7561 tggtgtagtt gaaagtttga ctcttgctac ctttggaaac tatattggtg cagaggtgac
     7621 cttgcctgaa cttaaaacag ctttgacagc tcaattaggc ggctttgtct tcacatctcc
     7681 tgaagaaatt gctggagtag agaaggttgg acaaacgaaa gcagacttta cactgactgt
     7741 caacggtgtg aagctaggtg gacacaagct tgacagtgca tttcaaggga cattggaaga
     7801 agtttaccca acagaattta cccaagcgaa agaactagaa gaagtaccag ctgtggcatc
     7861 agatgttgtg attaaagcca aaaaaaaggt tgaaaaacct gtggtttaca tcccagtctt
     7921 tccaggaacc aactcagaat atgattcagc taaggccttc gaaaaagaag gtgcagaggt
     7981 caatttggtg ccattcgtga ccttgaatga agaagctatt gtcaagtcag ttgaaactat
     8041 ggttgacaat atcgacaaga ctaatattct cttctttgct ggtggattct cggctgcgga
     8101 tgaaccagat ggttcagcta agtttatcgt caatatcctg cttaatgaaa aagtgcgtgt
     8161 ggctattgat agctttatcg cccgtggtgg tttgattatc ggtatttgta atggattcca
     8221 agccttagtc aaatcgggtc tcctacccta cggaaacttt gaagctgcta acagtactag
     8281 cccaaccctc ttctacaatg atgccaacca acacgtggcc aagatggtgg aaactcgcat
     8341 tgccaatacc aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc acgctattcc
     8401 tgtttcgcac ggtgaaggga agtttgtcgt gacggctgag gaatttgcag agctccgtga
     8461 caatggacaa attttcagcc aatacgttga ctttaacggt aaaccaagta tggattctaa
     8521 gtacaatccg aatggttctg tccatgccat cgaaggaatt accagcaaga atggtcaaat
     8581 catcggtaag atgggccact cagaacgtta tgaggatggt cttttccaaa atatcccagg
     8641 caataaagac caacacctgt tcgcatcagc ggttaaacat ttcactggaa aataagactt
     8701 acagattttc taatagatag tatcagtaat gtaaaagtca tgtaaatcta gctcttgatg
     8761 attacaaatg aaaattaggt ataaaaaatg acatacgaag taaaatctct taatgaagaa
     8821 tgtggtgttt tcggtatttg gggacatcca gatgctgcta agttgaccta ttttggactc
     8881 cacagtcttc aacaccgtgg tcaggagggg gcaggaatcc tctccaatga tcaaggacaa
     8941 ctgaagcgcc atcgtgacat ggggctttta tcagaagttt tcagaaatcc agctaatttg
     9001 gataaattga caggagctgg tgcgattggg catgtgcgtt atgcgactgc tggcgaagct
     9061 tctgtagata acatccagcc cttcctcttc cgtttttacg atatgcagtt tggcttggct
     9121 cataatggaa atctgaccaa tgcagcctct ctcaagaaag aactggaaca aagaggagca
     9181 attttcagcg cgacttcgga ctctgaaatc ttggctcacc tcattcgtcg cagtcataat
     9241 cctagcctga tgggcaaaat caaggaagcg ctcagccttg tcaaaggtgg ttttgcctat
     9301 atcttgctgt ttgaggacaa gttgattgcg gctcttgacc caaatggatt ccgaccgctt
     9361 tcgattggta aaatggctaa tggagcagta gtcgtttcct ctgaaacctg tgcttttgag
     9421 gtcattggtg ccgagtggat tcgtgatttg aagccaggtg agattgtgat cattgatgac
     9481 gagggcattc agtatgacag ctatacagat gatacccagt tggcgatttg ttctatggag
     9541 tatatctact ttgcccgccc tgattctaat atccacggtg tcaatgtcca tacggcacgt
     9601 aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat tgtagttggt
     9661 gtgcccaatt cttccctaag cgcggctatg ggatttgcgg aagaatcagg cttaccaaat
     9721 gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc gactcaagaa
     9781 ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt caaaggcaaa
     9841 cgtgtggtca tggtggatga ttccattgta cgtggaacaa cttctcgtcg tatcgttcag
     9901 ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc tgcactagcg
     9961 tatccatgtt tctacgggat tgatatccag acccgtcagg agctgattgc agccaatcat
    10021 acggtcgaag aaactcgcca aatcattggt gcggacagtc tgacttatct ttcaattgat
    10081 agcttgattg agtcgattgg tatcgaaaca gatgcgccga acggtggtct ctgtgtcgct
    10141 tactttgacg gtgactaccc aacgcctctc tatgactacg aagaagacta tcgtagaagt
    10201 ttggaagaaa agaccagttt ttacaagtag gcgacagatt ctccattaaa gaaaaggaaa
    10261 aaacaaatga caaataaaaa tgcatatgcc cagtcgggtg tggatgttga agcgggttat
    10321 gaagttgttg aacggattaa aaagcacgtg gcccgtacgg agcgtgcagg tgtcatggga
    10381 gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa agaacccgtc
    10441 ttgatttcag ggactgacgg tatcggaacc aagctcatgt tggctatcaa gtacgacaag
    10501 cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt
    10561 gcggaacctc tctattttct cgactacgta gcgacaggga agaatgaacc agctaagcta
    10621 gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt
    10681 ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt ggctggtttt
    10741 gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt agagggagat
    10801 gttcttctcg gacttgcttc aagtgggatt cactcaaatg gttactcttt ggttcgtcgt
    10861 gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag
    10921 gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccact catcaaagaa
    10981 gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt
    11041 atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt gcttccaatt
    11101 ttcaaaaccc ttgaaaaata cggtcagatt aaacacgaag aaatgtttga aatcttcaat
    11161 atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg
    11221 ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt
    11281 atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt caggtgattg
    11341 ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat gtgcttgagc
    11401 gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt gagagcaaga
    11461 cagactacga agtagccctt gtcgaactct tggaagaaca ccagattgac ttggtttgcc
    11521 tagcaggcta catgaaaatc gttggaccaa ccttattgtc ggcttatgaa ggtcggattg
    11581 tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt gaggatgctt
    11641 ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg ggtgtggata
    11701 caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc attgacagat
    11761 ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg aaggctctat
    11821 ttacagattg actttttgat gattcatatg atatctttga ttttaaattg gagtcagtgt
    11881 ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg
    11941 atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta agtttgaaac
    12001 aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga ttccattttc
    12061 agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atgttattta atctgatttc
    12121 ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta gtttaagaat
    12181 agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata ttttagcaat
    12241 aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtccgtg taggcttact
    12301 gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga tcaatatctt
    12361 aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt taattggtgt
    12421 ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca gcgtctcaga
    12481 caaagcgggc attgttgaat ttgcccaaga actcaaaaaa cttggttggg agattatctc
    12541 aacaggtgga actaaggttg cccttgataa tgctggggtg gataccattg ctatcgatga
    12601 tgtgactggt ttcccagaaa tgatggacgg tcgtgtgaag accctccacc caaatatcca
    12661 cggagggctt ctcgctcgtc gtgacttgga tagccacttg gaagcggcta aggacaacaa
    12721 gattgagctc attgaccttg tggtggtcaa cctttaccca tttaaggaaa ctatccttaa
    12781 accagatgtg acttatgctg atgcagttga aaatatcgat attggtgggc catctatgct
    12841 tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg gtagatcctg ctgactacgc
    12901 tgtggttttg gacgaattgg cagcaaacgg cgaaacctct tatgaaactc gccaacgttt
    12961 agcagccaaa gtatttcgtc acacagcggc ttatgacgcc ttgattgcag aatacttcac
    13021 agctcaagtg ggtgaaagca agcctgaaaa actcactttg acttatgacc tcaagcaacc
    13081 aatgcgttac ggtgagaatc ctcaacaaga cgcggacttt taccagaaag ctttgcctac
    13141 agactactcc attgcttcag ccaaacagct caacgggaaa gaattgtcat ttaataatat
    13201 ccgtgatgca gatgctgcta tccgtatcat ccgtgacttc aaagatagtc caaccgttgt
    13261 ggctctcaaa cacatgaatc catgtggaat tggtcaagct gatgacatcg agactgcttg
    13321 ggactacgct tatgagtctg acccagtgtc tatctttggt gggattgtcg tcctcaaccg
    13381 tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa tcatcattgc
    13441 accaagctat acggatgaag cgctagccat tttgatcaat aaaaagaaaa acttgcgtat
    13501 ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat acacaggtgt
    13561 agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag ctgactggca
    13621 agtggtgact aaacgtcagc caactgagac agaagcgact gctcttgagt tcgcttggaa
    13681 ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca tgacacttgg
    13741 tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg accaagccaa
    13801 agatcgtctg gacggggcgg tccttgcttc agatgccttc ttcccatttg cggataacgt
    13861 ggaagaaatc gccaaagcag gaattaaggc catcatccag cccggtggct ctgtccgtga
    13921 ccaagaatct atcgaagcgg cagacaaata tggcttgacc atggtcttta caggtgtgag
    13981 acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt ttttggctta
    14041 aaatgctaac tgaaacaaga ttaaaacgaa ctttttttga tataatgttg gtaaataatt
    14101 cgcaaaagag gttgaggaat gaaactgctt gttgtcggtt ctggtggtcg tgagcatgcg
    14161 attgctaaaa agttacttga atcaaaagac gtgaaaaaag tctttgtagc tcctgggaat
    14221 gatgggatga ctctggatgg tttggaattg gtaaatatct ctatttccga acattctaaa
    14281 ttgattgact tcgcaaagac caatgatgtt gcttggacct ttatcggtcc agatgatgcc
    14341 cttgctgctg gtatcgtgga tgattttaac caagctgggc ttaaggcctt tggtccgact
    14401 agggctgcag cggagctgga gtggtccaag gatttcgcca aggaaatcat ggtcaaatat
    14461 ggtgtttcga cagcagccta tggcacattt tcagatttcg aggaagcaaa ggcctatatc
    14521 gaaaagcatg gtgcgcctat cgtagtcaag gcggatggct tggcgcttgg gaagggtgtc
    14581 gtcgttgctg agacggttga gcaagcggtc gaagccgctc atgagatgct tttggacaat
    14641 aaatttggtg actcaggtgc gcgcgtggtt attgaggaat tccttgaagg agaggaattt
    14701 tcactctttg cctttgtcaa tggtgataag ttctacatca tgccaacggc tcaggaccac
    14761 aaacgtgcct acgatggcga caaagggcct aacacgggtg gtatgggtgc ctatgcgcca
    14821 gttccacact taccacagag tgtagttgat acagcggttg acaccattgt caagccagtt
    14881 ctagaaggga tgattaaaga aggtcgccct tatctgggag ttctttacgc agggcttatc
    14941 ctgacagctg atggaccgaa agtcattgag ttcaacgctc ggttcggaga tccagaaact
    15001 cagattatct tgcctcgctt gacctctgac tttgctcaaa atatcacaga tatcctggat
    15061 agcaaggagc caaatatcat gtggacggac aagggtgtga ctctgggtgt ggttgtcgca
    15121 tccaagggct acccgctaga ctatgaaagg ggcgttgagt tgccagccaa gacagaaggc
    15181 gatgtcatca cctactatgc aggggctaag tttgcggaaa atagcagagc actgctctca
    15241 aacggcggac gagtttatat gctggttacc acagcagata ccgtcaaaga agcccaagcc
    15301 agcatctacc aagaactatc ccaacaaaaa atagaaggac tcttctaccg aacagatatc
    15361 ggaagcaagg caattaagta aagatataag aataacgcgc cgtagtcgcc aaacacgata
    15421 atggtcgtcg tggtgaaaag accagaacag tgaatgttct ggtcaggggg aaacttggag
    15481 accgtaggct caaagtttag gaatgaaacc gaaggtttgc ttccgcctcc atcacctaag
    15541 accattatca aaaagaaaaa taaaaattca caaaatacgt taatgatcgt atggtttgcg
    15601 agcgttaacg agctaatata gaacaatcac cgccgttgtg aaagaacgat tggatgataa
    15661 tccaatcgtt cagggaaatt ggaagacctt gggtttccaa tttaggcatg agacaccttt
    15721 ggtggctgct gccgtccctc acaagctaag gtgattgttg aaaaagagga aaaaggagaa
    15781 gaaatgaaac cagtaatttc catcatcatg ggctcaaaat ccgactgggc aaccatgcaa
    15841 aaaacagcag aagtcctaga ccgcttcggt gtagcctacg aaaagaaagt tgtttccgcc
    15901 caccgtacac cagacctcat gttcaaacat gcagaagaag cccgtagccg tggcatcaag
    15961 atcatcatcg caggtgctgg tggcgcagcc catttgccag gtatggtcgc tgctaaaaca
    16021 atccttccag tcattggtgt acctgtcaag tctcgtgctc ttagtggagt ggattcactc
    16081 tattctatcg ttcagatgcc gggtggggtg cctgttgcga ccatggctat aggtgaagct
    16141 ggagcgacta acgcagctct ctttgccctc cgtctcctct ctgtagaaga taagtccatt
    16201 gcggatgcac ttgccaactt tgctgaagaa caaggaaaaa tcgcagagga gtcgtcaaat
    16261 gagctcatct aaaacaatcg gaattatcgg tggcggtcaa ctgggtcaga tgatggccat
    16321 ttctgctatc tacatgggcc acaaggttat cgcgctggat cctgcggcgg attgcccggc
    16381 ctctcgcgtg gcggaaatca ttgtggcacc ttataacgat gtggacgccc tccgtcagtt
    16441 ggcagaccgt tgcgatgtcc tcacttacga gtttgaaaat gtcgacgctg acggtttgga
    16501 tgccgttatc aaggatggac aactccctca aggaacagat ctgctccgca tttcgcaaaa
    16561 tcgtattttt gaaaaggact ttttgtcaaa caaggctcaa gtcactgtgg caccctacaa
    16621 ggtcgtgact tctagcctag acttggcaga tatcgacttg tcgaaaaact atgtcctcaa
    16681 gactgcgact ggtggctacg atggtcatgg acaaaaggtt attcgttcag aagcagactt
    16741 ggaagcagcc tatgcgctag cagactcagc agactgcgtc ttggaagaat ttgtcaactt
    16801 tgaccttgag atttctgtca tcgtgtcagg aaatggcaag gaggtgacgt ttttcccagt
    16861 tcaggaaaat atccaccgca acaatatcct gtctaagacc atcgtaccag cccgcatttc
    16921 tgaaagtcta gtagacaagg ctaaagctat ggcagtgcga atcgcagaac aactcaactt
    16981 gtctggaact ctctgtgtgg aaatgtttgc gactgatgat gacgttattg tcaatgaaat
    17041 cgccccacga ccacataact ctgggcacta ttctattgaa gcctgtgatt tctctcagtt
    17101 tgacacccat attctcggtg ttctcggagc accattacca gtcatcaaac tccatgcgcc
    17161 agccgttatg cttaatgtcc tcggtcagca tgtcgaggct gctgagcaat atgtgacaga
    17221 aaattcaagc gcccacctcc acatgtatgg taaaatagaa gcgaagcaca accgcaagat
    17281 gggacatgtg accttgttta gtgatgtacc ggatagtgtg gatgagcttg gggaagggat
    17341 tgatttttag gacaagtcta tgatacaaat tatcgttaat acatttattg aaaaggataa
    17401 gactggagca gttgttgaag tgttgtatgc cagtgctgac caagataagg tacaagctaa
    17461 atatgaagaa ctagctgcat aataccccga aaattattta gctatctatg atgtaccgct
    17521 ggatacggat ttgaatacac tagatcatta cccgtctgtg tttattgaaa aagaggagtt
    17581 tgagtagaaa tcttggttta cctagatagc ttattcccaa cagcttaaga agaaaggaaa
    17641 aattaacaca tgatcaaccg ttactctcgc cctgagatgg cgaatatttg gagtgaagaa
    17701 aataaatacc gtgcttggct tgaggtggaa atcctctctg acgaggcatg ggctgagttg
    17761 ggggaaatcc ctaaggaaga tgtggctttg attcgcaaga aggcggactt tgacatcgac
    17821 cgtattttgg aaattgagca ggagacgcgc cacgatgtgg tggctttcac gcgtgcggtt
    17881 tctgagactc ttggtgaaga gcgcaagtgg gttcactatg ggttaacttc taccgacgtg
    17941 gtggatactg cttatggtta cctctacaag caggccaacg acatcatccg tcgtgacctt
    18001 gaaaacttca ctaatatcat cgctgacaag gccaaggagc acaagttcac catcatgatg
    18061 gggcgtactc atggtgtgca cgctgagccg acaacctttg gtcttaaatt ggcaacttgg
    18121 tacagcgaaa tgaaacgcaa tatcgagcgt ttcgagcatg cagctgctgg tgtagaagct
    18181 ggtaagattt ctggtgcggt tgggaacttt gccaatatcc caccatttgt agaggagtat
    18241 gtctgcgata aacttggcat ccgtgcccaa gaaatctcta cacaagtcct tcctcgtgac
    18301 cttcacgctg agtactttgc ggttcttgcc agcattgcga cttcaatcga acgtatggcg
    18361 actgagattc gtggtctaca aaaatctgag caacgcgaag tagaagagtt ctttgctaaa
    18421 gggcaaaaag ggtcttcagc aatgcctcac aaacgcaacc caatcggttc tgaaaatatg
    18481 actggtctgg cgcgtgtcat tcgtggtcac atgattacgg cttatgaaaa cgtcgctctc
    18541 tggcacgaac gcgatatttc ccactcatca gctgagcgta tcatcacacc agatacgacc
    18601 attttgattg actacatgct caaccgtttt ggaaatatcg ttaagaactt gacagtcttc
    18661 ccagaaaata tgatccgaaa catgaactcg acttttggtc ttatctttag ccaacgggct
    18721 atgttgacat tgattgaaaa aggcatgacc cgtgagcaag cctatgactt ggtgcaacca
    18781 aaaacagcct actcttggga caaccaagta gactttaaac cacttcttga ggcagattca
    18841 gaagtaacat cacgtctcac acaagaagaa atcgatgaaa tcttcaaccc agtttattac
    18901 accaaacgag tggatgatat ctttgaacgt cttggactag gtgattaatt aaaaaataaa
    18961 cagcgagctt caatctcgct gtttattttt tatcgaaaag acttagtctt cttttctttt
    19021 agtgagtcca taggctgcta gtgtggacat gagtcctgcg actactagtc ctgcagaatc
    19081 gtgagttcct gtttcaggaa gttttttctc tgttaccaca ggagctggat cttgaggaag
    19141 agctttgctt tcctcagcag gagcagttga tggagctggt tggcttagga tttctagttt
    19201 tggtttttct tcagcaatag cggcttgtcc gttttcatcg cctacatgtg ttaccatagt
    19261 tccgacttcg actatttgag taacggcttc ctgtgctacg acactattta caagtgtttt
    19321 cacttcctta ccatcggcag aagtgctcac agagtagaag ttgctacgat gtccattgac
    19381 gcccttagta atgacttgtg tttttccttt gagtaagagt ggattttcac gagtcactgt
    19441 ggtaaatgga atttcttctt tttggatatc cagtctaggt tttacctcag tagttggtgc
    19501 aagaccactt tcatcaccct tgtgagttac aggagcgcca acttcaacca cttggtttat
    19561 aacttctttg gttacctggc tatcaaggac tgtttctgtt gtttttccat tttcagtgag
    19621 tacagagatg taatgagttc gttcaccttt gactcctgct gtgataatat tttcctgacc
    19681 agctgggagg ttaggatttt ctttcttgat aacttcaaat ggaatttctt cagttcttgt
    19741 gatgagttct ggtctggttt caacattggc agccacttca ttttcatcta ggcttcttga
    19801 atgagttgca gctggtttga ggccttgaag agcggctttt aggttggcta caagtgtgtc
    19861 aagctcagct tgtttattac ggttgaggtt gtaatttaga gctgttttag ctgcgtcaag
    19921 ggcctcaaga ctttctttac tatatccttc taagtttgta ggaattttag ctaattcttc
    19981 gcggagagca ttataattag cacggaagta gtctttgttg tggtctgcaa aggcagtcat
    20041 gagttcaaag atttcctctt ccttgtattc agcgcttggt ctatctgccc agattgaaag
    20101 catacttccg actgttggaa gatctacttc aggatatttg gtagaagcta gttgattgaa
    20161 tggaacttta ccagaatttt caactgcttt tgaaagaggg taagcttcct cttgtttgtg
    20221 attgcctata acataatacc agtcaccgtt ggtattcaag aatttatagc ctttgcttgc
    20281 taggtattga ggtgatgcga ggttatatcc ccaccagcct ttagaccagt aagaaatcaa
    20341 gacatctttg tcaaactgaa catcgtcctt gtcttcatag tagaagccat cgttgaaggc
    20401 cattggttga agccctcttt ctttggccat agctgcgagg gtgttggcat attcggcaaa
    20461 tttgccatag agttgatacc acttgaggta gtaccagcct tgggcactag tcgcatcgtt
    20521 ggcgtattcg tcagtaccaa agttgaaaat ctttgtttta cctgcaaaga agtccatgta
    20581 tttaccgatg agggctttta caaagtccat cgcttcttcg tttttcaagt ccatagttgt
    20641 ttttgaaact ttatcaaagt gggcttgagg atttttaata cctaattttt ccatggcaac
    20701 cagcatagca tccatgtgac ctggactgtt aatagctggg atgagaccga tgtccttaga
    20761 tttagcgtat tcaattagct ctgtcacttc tgcctgtgtt agtgcagtac cgtttggatc
    20821 gtcgtagtaa gctttagttc cttcgataat agctttttta acgtcatcac tagcataggt
    20881 ttttccgttg gcagtaatgg tcatatcatt gagtagaaag cgaagtccgt catttcctag
    20941 aaggagatgg acatcagaat atccgagctc actggccttg tctacgatgc gtttgagctg
    21001 gttcagagta aagtatttgc gtccagcatc gattgagatt accttgtttt tggcaagttt
    21061 ttcaacctca cgtttagctt cttcttcttt ttgagcttca ggcgtgaggg tcaagttgtt
    21121 gacagtttct tgaagtttag caatggcttg atcaatcgta tcttgttggg cacggctaag
    21181 gttgctatcg agagagcgaa tagctttttc agcttctttt acggccgcga cgctttctgc
    21241 agtataacgg ttcaggtctt ttggtacctc gttaagtgct tgctctgcag attcataatc
    21301 agctgcgaag tattcagcgt tggcatttgc aaaatgacgc atgagtttga agaggcgtga
    21361 tggtgaataa cgtgcagatg gagtgtcagc ccaagcagct accataccac cgatgattgg
    21421 gatatcagct ccttctgttt ttggtacaga agtgattggt gtgtttttaa taccattgag
    21481 cccctgatcg agattgtacc agccttggcc atcagcgttt cgtccaagaa cgtagtacca
    21541 agcatcattg gtattaagga tttggtgacc tttttcagct agtagtttag aagaagcgac
    21601 atcgtagcct ccccaaccac cagtccacat agaaacgatg atgtctttgt caaaactacc
    21661 aaagcttgtg tcgctattgt agtagatacc gtcgttaaag gccattggtt tgagaccgtg
    21721 cgattttaca atacgagcga ggtcattggc gtaggcaata aatttttcat agccttttac
    21781 agggtagcct tcgtttggat agtatttatc agcttgaagc acactccaac ctttagcatc
    21841 tgtcgcatca ttggcatatt catcaagtcc gatgttgaag atttcagtct ttttcgcgaa
    21901 ataagcagca tacttgtcga taagggcttt tgtaaaagcg acagcttgtt cgttgtcaag
    21961 atcgacagta cgggctgatt tcttcccaaa atagctaaag ttagggtttt ggattcccaa
    22021 ttctttcatg gcattgagaa tcgcatccat gtgtccagga ctatttactg tcggaatgag
    22081 accgatacct ttatctttgg catagttaat cagatctgtc atttgacttt ctgttaagtg
    22141 attgccgttt ggatcgttgt aataatcatt tgtacctttt tcaatggcgc gtttgacatc
    22201 gtcactggca taggtcttgc cgttagctgt gatgctcata tcgtccaaca tgaaacggag
    22261 tccatcattt ccgactaata ggtgtaaatc agtgtagcca taatgtttcg ctttatcgat
    22321 gatttccttg agctgttctg gtgagaaata tttacgtcca gcatcaatag aaacaatttt
    22381 ctttttcgct agtttttcat ttacagttgc agcacgttcc tttcctgcct ctgtttccgg
    22441 tttgtcagcc tctgctttcg cttcatcttt tttagctggt ttatccttgt cagtcttgtc
    22501 tgtatttgac tctttagaat caacctcttt cgcttcttcc tttttagggc tagcttcttc
    22561 tgccttttta ttagcagttt ctttttcagc agaagttgga gttaccactt ctgctttatc
    22621 actaggagtt gaactaactt cctcttgtgg tttttcttct gtttttggaa gactagctac
    22681 cttatcagta gctggagttt ctgtttctac agtttttgga gcttctggtt gaagctctgc
    22741 tttaggtgtt tcctcagtcc gattttcgga tgattgaggg gaatcagaaa ccgtatggat
    22801 ggtcggttgg ttttctgtag tagtaggagt aactccatcg gctgcaacag tctgtgcttg
    22861 gaaggcaaat ccaattagaa cagaagctgc tcctacagcg tatttacgaa tagaaaaacg
    22921 ctgttgtttt tcatgtttca ttgcaaaacc tcctgattgc attgttatat tgatagcgat
    22981 tatataaatc aacgccttta ttttatttct tatattaacg agagtcaaga ggagatgaca
    23041 aaaaactata ataagtataa aaaaatataa aatttaaact taagatttca gattggtcga
    23101 aaatacatat atatatctag tataattttt ggttctattt ctataaaata ttccacaaat
    23161 tatagaatct tcccaaaata ggtaagcgct acctttttgg tgtggtataa taagcataga
    23221 aaaagcccga gcgattaggc tcaggctttc ttcttagtga tcacggtcac atgagatgaa
    23281 tttaatcttg tagtaatcag atcgtttgta agtttcactg tattctaaaa cttggccagt
    23341 tgattcgagt ttggtgattt tagtttgtag gacagtaggg aattgttcat cgactccgag
    23401 gactgaagcc gcatgttctg gagttggaaa gactatttcg ttgatttctt caaagtgttc
    23461 atcattcatg tgaatgtggt agtctaactt gaaacgatta tagatagaac tatagtattc
    23521 aaggtttgga taatttgcgt tgatatattg ttctgggatg taggatgtat ggtagatata
    23581 aacgacaccg tttgattcgc ggatacgttc aatcttgtag tagaattgat cgccgcgtag
    23641 acccaatttt tccaagtaaa caagcttgtt tccgcgttca attgaaagaa cagttacctt
    23701 atcatcttta gcattgaaga gttcaatatc tgaaaactct acaagcttgt gtttgcgtgc
    23761 acgtgaaacg aaggttcctt ttccttgttg gcggacaata tagccatctt tggcaaggtc
    23821 gtttaaggcg cgaacaactg tgatagagct gacatcgtac attgaaatga gttctgcttc
    23881 agtgtaaaat ttatctccac tgctaaactg cccagagatg attttatttt ttaattcgtc
    23941 ttttatgtat tgatatttgg gaattgccat atttttacct ctattttcct tctccataga
    24001 aaattttacc ataaaagcga aaaaaatagt agtcttttga ataaaaaatt agaataatag
    24061 tttgaaaaat taagcgatat agggattgct catattttat aaatgtattc tatacaaaat
    24121 tattttaggt cctatattcc gaatttaaag agcgtttgaa atgggtctaa aaatgtttga
    24181 aactaattga atgtgtgtat aatagtaaaa atgcagtaaa tatcccgaca atttctattg
    24241 acaattcaaa cagattggtt tataattaat ataacaacaa atgaaagcgc aaactttcgc
    24301 ggtcggaagg tagttttatg acacgatttg agatacgaga tgatttcttt ctcgatggaa
    24361 aatcatttaa gattttatct ggtgccattc attattttag ggttcctcca gaggattggt
    24421 atcattcgct ctataacttg aaggctcttg gttttaatac ggtagagact tatgttgctt
    24481 ggaatttaca cgagcctcgt gaaggtgagt ttcattttga aggtgatctg gatttagaga
    24541 aatttctcca aatagcgcag gatttgggtc tctacgcaat tgtgcgtccg tctccattta
    24601 tctgtgcgga atgggaattc ggtggcttac cagcttggct cttgaccaag aacatgcgaa
    24661 ttcgctcatc cgacccagca tatatcgagg cagttggtcg ctactatgat cagttattgc
    24721 caagactggt gcctcgtttg ttggacaatg gtggcaatat tctcatgatg caggttgaaa
    24781 atgagtatgg ttcttacgga gaagataagg cttacctgag agcgattcga cagctaatgg
    24841 aagagtgtgg cgtaacctgt cccctcttta catcagatgg tccatggcga gctactctga
    24901 aagctggaac cttaattgaa gaggacctct ttgtaacagg aaactttggt tctaaggcac
    24961 cttacaactt ttcgcagatg caggaattct ttgatgaaca tggtaagaaa tggccactca
    25021 tgtgtatgga gttctgggat ggttggttca atcgctggaa agaaccgatt atcacacggg
    25081 atcctaagga attggcagat gcagttcgag aggttttgga acaaggctct atcaatcttt
    25141 acatgttcca cggtggtaca aactttggtt tcatgaatgg ttgctcggct cgaggaactt
    25201 tggacctgcc acaagttacg tcttatgatt acgatgccct tctggatgaa gaaggaaatc
    25261 caactgctaa atatcttgca gtcaagaaga tgatggcaac acatttttca gagtatccgc
    25321 agttggaacc actctacaaa gagagtatgg agttggatgc tattccacta gttgaaaaag
    25381 tttctttgtt tgaaacctta gatagcttgt caagtcctgt agaaagtctc tatcctcaaa
    25441 agatggagga gctgggacaa agttatggct acctacttta tcgaacagaa acaaactggg
    25501 atgcagaaga agaaagactt cgtatcattg atggtcgaga tagggcccag ctgtatgtcg
    25561 atggtcagtg ggttaaaact caatatcaga cagagattgg ggaagatatt ttttatcaag
    25621 gtaaaaagaa agggctatct aggttagata tcttgataga aaatatgggg cgtgtcaact
    25681 atgggcataa gttcttagcg gatacgcaac gtaagggaat tcggacaggg gtctgtaagg
    25741 atctgcattt cttactaaac tggaaacact atccactccc actagacaat cctgagaaaa
    25801 ttgatttttc aaaaggatgg actcaaggac aaccagcctt ttacgcttat gactttacag
    25861 tcgaagagcc aaaagatact tacctagact tgtctgagtt tggtaagggg gttgcctttg
    25921 tcaatgggca gaatctagga cgtttttgga acgttggccc aactctctca ctttatatcc
    25981 ctcatagcta tctcaaggaa ggtgccaacc gcatcattat ctttgaaaca gaaggtcaat
    26041 ataaagaaga gattcattta actcgtaaac ctacactaaa acatataaag ggggaaaact
    26101 tatgacaatt gtaggatgcc gtattgatgg acgtttgatc cacggacaag tagccaatct
    26161 ttgggctgga aaactaaatg tttcacgcat tatggttgta gacgacgaag ttgtcaacaa
    26221 cgatattgaa aagagtggtt tgaaacttgc gacaccacca ggtgtgaaat tgagtatttt
    26281 gccagttgag aaagctgcag ccaatattct tgctggcaaa tacgatagcc aacgtctctt
    26341 tatcgtggct cgtaaaccag accgcttcct tggtttggta gaagcaggtg taccacttga
    26401 aacccttaat gttgggaata tgtctcaaac accagaaact cgttctatta cacgttctat
    26461 caacgtagta gacaaggatg tggaagactt ccacaaactg gcagaaaaag gtgttaaact
    26521 tactgctcag atggttccaa atgatccaat ttcagacttt ttgagcttat taaaatagga
    26581 aaaaaatttt taggaggtca ttgttatgat acaatggtgg caaattttac ttctcacttt
    26641 gtactcagct tatcaaatct gtgatgagtt gacgatcgtt tcatctgcag gttcccctgt
    26701 atttgctggt ttcattactg gtttaatcat gggagatgtg actactggtt tacttatcgg
    26761 tggtaacttg caactgttcg ttcttggggt tggtaccttc ggtggtgctt ctcgtatcga
    26821 cgcaacttct ggtgcggttc ttgcgacagc cttctctgtt tcacaaggaa ttgatgcacc
    26881 gcttgccatt actacaatcg ctgtaccagt agcagctctc ttgacttact tcgacgttct
    26941 tggtcgtatg actactacct tcttcgctca ccgtgtggat gctgcaatcg aacgctttga
    27001 ctataaaggt attgaacgca actacttgct tggtgcgatt ccgtgggctc tatctcgtgc
    27061 ccttccagtc ttctttgccc ttgcttttgg tggtgccttt gtacaatcag tagtagactt
    27121 cgttgaagcc tacaaatggg ttgcagatgg cttgacactt gcaggacgta tgcttccagg
    27181 tcttggattt gcaatcttgc ttcgttacct tccagttaaa cgtaaccttc actaccttgc
    27241 tatgggattt ggtttgacag ctatgttgac tgttctttac tcatatgtaa caggtcttgg
    27301 tggcgctgtt gctggtatcg taggtactct tcctgctgaa gttgctgaaa aaattggttt
    27361 cgtgaacaac ttcaaaggtt tgtctatgat tggtatttct atcgtaggta ttttccttgc
    27421 agtgcttcac ttcaaaaata gccaaaaagt agctgtagca gcaccttcta caccatcaga
    27481 aagtggggaa atcgaagatg acgaattcta attacaaact tacaaaagaa gattttaatc
    27541 aaatcaacaa acgtagcttg tttactttcc aattaggttg gaactacgaa cgtatgcaag
    27601 cttctggtta cctttacatg atcttgcctc agttgcgtaa aatgtatggt gatggaactc
    27661 ctgaattgaa agaaatgatg aaagttcata ctcaattctt caatacttca ccattcttcc
    27721 ataccattat cgctggtttt gaccttgcca tggaagaaaa agatggtgta ggttcaaaag
    27781 acgccgttaa cggtatcaag acaggtttga tgggaccatt cgctcctctt ggggatacaa
    27841 tctttggttc acttgtacct gctatcatgg ggtcagtcgc agcaactatg gctatcgctg
    27901 gccaaccttg ggggatcttc ctttggattg cagttgcagt agcgtatgac atcttccgtt
    27961 ggaaacagtt ggaatttgct tacaaagaag gggttaacct tatcaacaac atgcaaagta
    28021 ccttgacagc tttgattgac gctgcatctg tacttggtgt cttcatgatg ggtgctcttg
    28081 tagcaacagt gattaacttt gaaatttctt acaagttgcc aatcggtgaa aagatgattg
    28141 atttccaaga catcttgaac caaatcttcc cacgtttgct tccagcaatc tttactgcct
    28201 ttatcttctg gttgcttggt aagaaaggta tgaactctac taaagctatc ggtattatta
    28261 tcgtacttgc tttggctctt tctgcccttg gtcactttgc acttggaatg taattcctta
    28321 tgactaaatc attaattttg gtgagccatg gtcgcttctg tgaggagctt agaggtagca
    28381 cagaaatgat tatgggccca caagacaaca tttacacagt agctcttctt ccagaagatg
    28441 gcccagaaga atttactgct aaatttgaag ctgttattga aggattggat gatttcctag
    28501 tttttgcgga tcttctcggt gggacacctt gtaatgtggt gagtcgcttg atcatggaag
    28561 gtcgtgatat tgacctttac gcagggatga atcttccaat ggtgattgaa tttatcaatg
    28621 cgagccttac aggcgcagat gcggactaca agagccgtgc tgcagaaagc attgtgaaag
    28681 ttaatgacct gttagcgggc ttcgatgatg acgaagatga ataatactct tcgaaaatct
    28741 cttcaaacta cgtcaacgtc gccttactgt aggtatatgt tactgacttc gtcagtctta
    28801 tccggcaacc tcaaaacggt gttttgagct gacttcgtca gtcttatccg gcaacctcaa
    28861 agcagtgctt tgagcagcct gcggctagtt tcctacagat tttagttgga actcgattca
    28921 attcatgtga caacgtgaaa atcgttagag cattttatat agaatataca tgggaatgga
    28981 gcttactccc attcctatat ttaatagaaa aagaggaact caatgctaca ttatacaaaa
    29041 gaagacttgc tcgaattggg tgcagaaatc actacgcgtg aaatctacca acagcctgat
    29101 gtatggagag aagcttttga attttatcaa gcaaaacgtg aagaaattgc agccttccta
    29161 caagaaatcg ctgataaaca tgactatatt aaggttatct tgacaggtgc tgggacttct
    29221 gcttatgtgg gagacacctt gctaccttac tttaaggaag tctatgacga acgcaaatgg
    29281 aatttcaatg ctattgcgac aacagatatc gttgccaatc cagcaaccta tttgaaaaaa
    29341 gatgtggcaa ctgtccttgt gtcttttgct cgtagtggga actcgcctga aagtgtggcg
    29401 actgttgatt tggccaaatc cttggtggat gagctttatc aagtgacgat tacttgtgca
    29461 gcagatggta aattggctct tcaagctcac ggtgatgacc gtaatctctt gctcttgcaa
    29521 ccagctgtct ctaatgatgc tggatttgcc atgacttcta gctttacgtc tatgatgttg
    29581 acagctctct tggtctttga tcctacagaa tttgctgtta agtctgaacg ttttgaagtt
    29641 gtatctagtc ttgcccgtaa agttttagac aaggcagaag atgtcaaaga gctcgttgat
    29701 ttagacttta accgtgtcat ctatctaggc gctggtcctt tctttggact tgctcatgaa
    29761 gctcagctca agattttgga attaactgct ggtcaagttg cgaccatgta tgaaagccca
    29821 gttggcttcc gtcacggtcc aaaatctctt atcaacgaca atacagttgt tttggtcttt
    29881 ggtacaacga cagactacac tcgtaagtac gacttggact tggttcgtga agttgctggt
    29941 gaccagattg ctcgtcgtgt tgtgcttttg agtgatcaag cttttggtct tgaaaatgtc
    30001 aaagaagtgg cccttggttg tggcggtgtc ttgaatgata tttaccgtgt cttcccttac
    30061 atcgtttatg cccaactctt tgccctattg acttcactca aggtagaaaa taaaccagat
    30121 acaccgtctc ctacaggtac agtaaaccgt gtagtacaag gtgtcataat tcacgaatat
    30181 caaaagtaag acagtgttta tgaattcttg acaagaggat ttgtaaatta tcagataaac
    30241 catagattgt cagtacgctt tctatggttt gtttgcttga gagaaatagt aaaaggagaa
    30301 cagaatgaaa gcatacacag agcgtgtatt tggaaatgtt gagggcgagg atgtcttggc
    30361 ctatcgattt gagacagacg gtggctacca acttgaggtt atgacttatg gtgcgaccat
    30421 cttgcgctat gtcgcacctg acaaggctgg aaattttgcc aatgttatct tgggatttga
    30481 tgactttgat agttatgtag gcaatagtcc caagcatgga gcaagtgtag gtcctgtagc
    30541 gggtcgtatt gcaggtgcga cctttgagct caatggtaag acctatgacc ttgaggttaa
    30601 taatgctagc aactgtaatc acagtggttc aactggttgg gattccagct tgtttgaagt
    30661 tgaagaagta agcgatcatg gcttgactct ctacacagag cgtacagatg ggacaggagg
    30721 gttccctgga aatctcaaga tttggatcag ttatcacttg gaagaaactg gtgcctatga
    30781 aatcagctac aaggtaacga ccgatcagga tacgctggtc aatccaacca accacagcta
    30841 tttcaacttg tctggtgatt tcacgcagac gattgaccgt catgtcttcc aactaaacac
    30901 agagggcatt tactcaatcg ctcctgacgg tgttcctgcc aaaactccag aagccaaccg
    30961 tgatgtggtc aaacacatct acaatggtac cttgttgaag gatatctttg cagaagaaga
    31021 tgagcaaatc cagctggcat caggtttgga tcacccattt gcccttcctg taggccatga
    31081 caatgctgga ttcctttatg accaaaattc aggtcgcttc ctgcttttca agacagaagc
    31141 tccttgcttt gtggtctaca cagcaaactt tgtggatgaa agtgtcatca taggaggtca
    31201 gccaatgcta cagcacaatg ggattgctct tgaagcacaa gctttaccag atgccattca
    31261 cagtgacctt aaaggccaag tcattcttaa agctggtcaa accttcacca gtaagacacg
    31321 ttatgaactt gttgtgaaat aaaaaaagct ggtcaaaaag tctttaaaat taaaataatg
    31381 catattgtca ggtgatggaa agtttgataa tatgcgtttt attataaaat gatttacttt
    31441 atcttatcct gaaatggtgt tatttttgag ttatctggct tgactttctt gatgaaaatt
    31501 atataatagt tgtaacaata ataattacaa cgagaaaaga ggaccattta tgacctatga
    31561 atacaagagt cacatttatt tggcagagac agctttaaat gtaaaggatt tggccagtca
    31621 aacagccttt tatcagcaag ttattggttt agaaatctta tctcaaactg agacagagtc
    31681 cattctaggc cttggtggaa aagtcttggt acagctgatt caagcacaag agagcggaga
    31741 agtgagggaa cattatggtc tttaccattt ggccattctc ttgcccacac gcaaggcttt
    31801 ggctgatgtc ttgaaacacc tgacagattt acagattcct cttgtcggcg gtgcagacca
    31861 tggttacagt gaagctattt acttggaaga tttggaggga aatggcattg aactctatcg
    31921 agataagcca gtttccacat gggatattcg agaagatgga cgcattatcg gagtgacaga
    31981 ggctcttgcg acgcaggaca tttatgagtt gggggaaaga gtagagccct ttatcctggc
    32041 agaaggtacg agaatggggc atattcatct ttccgtcaag gatagtcgaa agtccagcca
    32101 gttttatcaa aaggtgttag ggctagagga taaattcagt gtgcctagcg ctagttggat
    32161 tgcggctgga gattaccatc atcatttagc agttaacgaa tggggaggaa aaggtctggc
    32221 ttcgcgtaag caaggcttac caggtttagc ctactatgtc atcgaagtcg cacataaaaa
    32281 agaactatta acgattgccc aacgagcaca agaagttgac gcaccaatca aatggatgac
    32341 atcgagccaa ttggaaatca cagactcaga tggcatcgtg acccgtattc gtttagcaag
    32401 gtaaaattag gaatttttaa taagttataa gtattgctag tttgcttttt gaacggtctt
    32461 tggtaacaga actgcgtgag ctggatgggc gagaattttg acaaagaagc tgattagaga
    32521 tataatgaag aaaaatcgag caaggaaaaa gaaatgacaa attcaaagta tattacttgt
    32581 ttgaaacgtt cagagggcca gttgtgtggg attcaaaaga tgattgaagg agatcgtgac
    32641 tgtgctgata ttgtgacgca actgacagca gtgagatcta gtgtagagcg cgtgattgag
    32701 atgataatta ccgaaaatct tactgaatgt atcaatcagc cactagatga ttctgaagct
    32761 caaaaggaac gcctagaaaa ggctatccga tacttgatta aacggaaata aatgaatgtt
    32821 ttgagacgat atgtgcaaag gtctattaac tattagaata gtttaatgtg agtcaaaaga
    32881 agttgtaaaa aatctttaaa attaaaaaaa cgcatagtat caggtgttga atgtactgac
    32941 cccaaaaagt tagatttttt ctgtctaact tttggggtgc agttcataag aaccttggta
    33001 atatgcgttt
//
LOCUS       DAVKZB010000011        31689 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKZB010000011 DAVKZB010000000
VERSION     DAVKZB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.011"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            326..963
                     /locus_tag="VXA80_000464"
                     /pseudo
     CDS             326..963
                     /locus_tag="VXA80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1013..1480)
                     /locus_tag="VXA80_000465"
     CDS             complement(1013..1480)
                     /locus_tag="VXA80_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0353010.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLKSSRG"
     gene            complement(1500..2675)
                     /locus_tag="VXA80_000466"
     CDS             complement(1500..2675)
                     /locus_tag="VXA80_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0353011.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDAVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEKNREKS
                     LKNALKTLLPERLAEFFAQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(2734..3579)
                     /locus_tag="VXA80_000467"
     CDS             complement(2734..3579)
                     /locus_tag="VXA80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0353012.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            3704..4261
                     /locus_tag="VXA80_000468"
     CDS             3704..4261
                     /locus_tag="VXA80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0353013.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            4280..4747
                     /locus_tag="VXA80_000469"
     CDS             4280..4747
                     /locus_tag="VXA80_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV0353014.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLIIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            4836..5465
                     /gene="upp"
                     /locus_tag="VXA80_000470"
     CDS             4836..5465
                     /gene="upp"
                     /locus_tag="VXA80_000470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV0353015.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            5639..6229
                     /gene="clpP"
                     /locus_tag="VXA80_000471"
     CDS             5639..6229
                     /gene="clpP"
                     /locus_tag="VXA80_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV0353016.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            6308..6556
                     /locus_tag="VXA80_000472"
     CDS             6308..6556
                     /locus_tag="VXA80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV0353017.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            6657..7817
                     /locus_tag="VXA80_000473"
     CDS             6657..7817
                     /locus_tag="VXA80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0353018.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLVSPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGRDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            8085..8954
                     /locus_tag="VXA80_000474"
     CDS             8085..8954
                     /locus_tag="VXA80_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0353019.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGAISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFEMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            8958..9914
                     /locus_tag="VXA80_000475"
     CDS             8958..9914
                     /locus_tag="VXA80_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0353020.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            9914..10678
                     /locus_tag="VXA80_000476"
     CDS             9914..10678
                     /locus_tag="VXA80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353021.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            10678..11388
                     /locus_tag="VXA80_000477"
     CDS             10678..11388
                     /locus_tag="VXA80_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353022.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            11747..12403
                     /locus_tag="VXA80_000478"
     CDS             11747..12403
                     /locus_tag="VXA80_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0353023.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            12511..13606
                     /gene="prfB"
                     /locus_tag="VXA80_000479"
     CDS             join(12511..12582,12584..13606)
                     /gene="prfB"
                     /locus_tag="VXA80_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV0353024.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            13624..14316
                     /gene="ftsE"
                     /locus_tag="VXA80_000480"
     CDS             13624..14316
                     /gene="ftsE"
                     /locus_tag="VXA80_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV0353025.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            14309..15235
                     /locus_tag="VXA80_000481"
     CDS             14309..15235
                     /locus_tag="VXA80_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353026.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            15521..17701
                     /locus_tag="VXA80_000482"
     CDS             15521..17701
                     /locus_tag="VXA80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0353027.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            17753..18568
                     /locus_tag="VXA80_000483"
     CDS             17753..18568
                     /locus_tag="VXA80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0353028.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            18708..20051
                     /locus_tag="VXA80_000484"
     CDS             18708..20051
                     /locus_tag="VXA80_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0353029.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            20266..21456
                     /locus_tag="VXA80_000485"
     CDS             20266..21456
                     /locus_tag="VXA80_000485"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV0353030.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLNLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            22009..22944
                     /locus_tag="VXA80_000486"
     CDS             22009..22944
                     /locus_tag="VXA80_000486"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV0353031.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            22978..24015
                     /locus_tag="VXA80_000487"
     CDS             22978..24015
                     /locus_tag="VXA80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0353032.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKVDSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            24188..24793
                     /locus_tag="VXA80_000488"
     CDS             24188..24793
                     /locus_tag="VXA80_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV0353033.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            24950..25480
                     /locus_tag="VXA80_000489"
     CDS             24950..25480
                     /locus_tag="VXA80_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV0353034.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            25501..26586
                     /gene="rlmN"
                     /locus_tag="VXA80_000490"
     CDS             25501..26586
                     /gene="rlmN"
                     /locus_tag="VXA80_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV0353035.1"
                     /translation="MKPSIHSLPHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            26586..27068
                     /locus_tag="VXA80_000491"
     CDS             26586..27068
                     /locus_tag="VXA80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0353036.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVRVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            27070..28611
                     /locus_tag="VXA80_000492"
     CDS             27070..28611
                     /locus_tag="VXA80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0353037.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(28667..29470)
                     /locus_tag="VXA80_000493"
     CDS             complement(28667..29470)
                     /locus_tag="VXA80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV0353038.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(29553..29774)
                     /locus_tag="VXA80_000494"
     CDS             complement(29553..29774)
                     /locus_tag="VXA80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353039.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(30253..30450)
                     /locus_tag="VXA80_000495"
     CDS             complement(30253..30450)
                     /locus_tag="VXA80_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353040.1"
                     /translation="MLSVNTILEKFYKEHQAKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            30620..30892
                     /gene="rpsP"
                     /locus_tag="VXA80_000496"
     CDS             30620..30892
                     /gene="rpsP"
                     /locus_tag="VXA80_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV0353041.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            30912..31151
                     /locus_tag="VXA80_000497"
     CDS             30912..31151
                     /locus_tag="VXA80_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV0353042.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT         9631 a         5852 c         6894 g         9312 t
ORIGIN      
        1 gaaagatgta cgacttatcc cgtgaggtct catgagcgct gaaagcgtag taacaacgaa
       61 tcatgagaag tcagccgaac ccatagtagt gaggaaactt ctgtaatgga agtggagcga
      121 aggggagaat actcaaacag tctggggaga gactgtttga gatctgtcgc tagaaagaga
      181 aaacgacaga tcgaagtaat cctacttcac ttgtgtctgg aaaacgaacg gtctgataga
      241 actggactct gtcacgtatt gactagatga aggttcacca atataagatg tccctcaggc
      301 accaaaacaa gaaaggaata cgcacatgtc aaaattgcta gataaaatat tatcacgtga
      361 caacatgctt gaagcctaca atcaagtaaa atccaataaa ggctcagctg ggattgatgg
      421 aatgactatc gaagagatgg atggctatct cagacaaaac tggcgcctga cttgagacca
      481 agtaaactga agtatctagg atttggattc tggaagtcag ccgaggggtg gaaaagtcgc
      541 ccgcaccaag acagtgtaca gaatttcaag agaaaactca agaaactgac tagccgaaag
      601 tggagtattg acttggataa tcgtattgag aaactcaatt ggcttatccg aggctggata
      661 aactactttt caatgacgaa catgaaatca gtcatggaga gcattgatga acgactgaga
      721 acacgcataa gagtgattat ctggaagcaa tggaagaaga aatctagacg attatgggga
      781 ttgcttaact taggggttcc taaatggata gcagataagg tatctggctg gggcgaccat
      841 tatcaattag tagctcagaa gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa
      901 aaacgtggac tggtttcgtg cttggattat taccttgaac gacatgcgtt aaaagttagt
      961 tgaaccgccg tatgccgaac ggcacgtacg gtggtgtgag aggggctaga gattatcccc
     1021 tactcgattt caaatcataa agccagtcgt caccgttttt gtagtaaaag aattcactga
     1081 gatcttcttc tagaaacaca cgaagcatat cagacatatc atcggttgca agttttagat
     1141 gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc gtctgaagac tggagttcac
     1201 cagtaaagtg ttctgtcttg taaaaaagga cgacataacg ataatccttg tcgtcatacc
     1261 agtttttgat accacagagt tggggtttgg aaatgatcag accagtttct tctttcactt
     1321 cacgaatgac agcatcgaca aaggattcgc cacgttcaac atgaccacca ggaaaagtaa
     1381 tgccaggcca gtcgggacta actcggtctt ggaccaggac cttatctccg tttttaatca
     1441 tacacatgtt aacaaattcg actgcctctc ttctgttcat tcttcacaac ctttaatctt
     1501 taatcataat gcagacttcc cgccacccag ccggtacaga gggcagaagt gatgttaaag
     1561 ccacccgtgt gggcattgat atccataact tcgcctgcaa agtggaggcc aggtaccagc
     1621 ttactttcaa gggttttagg attgatttcc ttgagactga ctccaccctt ggtaacaaag
     1681 gactttgcaa gggacatttt tccagttaca ggaattttaa gttctttaat ggactggaca
     1741 agttgttctc gttccttttc agtcagctgt ttgacttttt caggatatcc ttgtgcaaaa
     1801 aattcggcca agcgttctgg taacaaggtt tttaaagcgt ttttcaagga tttttcccga
     1861 tttttttcta gaaatgtaac caagtccttc tcagaaagtt gaggcaaaac atcgagtgag
     1921 agaacttccc cacctttgac aaagctagac atgcgtaggg cagcaggacc tgacaaacca
     1981 aagtgggtaa agagtaaatc atgagtgatg acatgcttac cataacttag ggtcacagcg
     2041 tccagagaaa tcccttgtaa ggctttatgt ggaaaatctg ttaataaagg actttcagca
     2101 gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa tctcgtgacc aaaaccagtc
     2161 gaaccagtcg aaggataaga cttaccacct gttgtgacaa tgagtttctc acaagtgaag
     2221 gtttgatccg ctgacttaag gacaaactgg tcatctactt ttttaacaga aacgatttct
     2281 atttgagtag caacttgacc acctagttcg gtgattttct tttccaaagc ttcgataata
     2341 gtccgagact tgtcactggc tggaaagacg cgtccgtggt cttcgacctt aagtttaaca
     2401 ccattttctg taaaaaagtt gatgatgtca tgattatcga actgggagaa aacactgtaa
     2461 agaaagcgtc cgtttccagg aattccagct agcaggttgt ctaagctacc attgttggtc
     2521 acattgcaac gtcccccacc agtcccagct aatttttttc caagtttccg atttttttcg
     2581 atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg tagccatcat accagcaggt
     2641 cccccaccga tgacaatagt atcaaaatgt ttcatagctc tattgtacca caaaaaaaca
     2701 agagatgatg gtcacctctt gtcaagaatg caattaatca atttcatagc ccatcagcaa
     2761 accgccctct tctgcataga aactgcagag accagaggtt ggtagaattt taatatccgc
     2821 ttgtgggaag gtttcacgga ttcgctctga gagctgttga caacattttt cgttattgcg
     2881 ttgggccatg acaatacggc caccagcata tccagctttt actaactcat cataggcagc
     2941 ttgaactgat ttctttgatc cccttgcttt ttgtagcaat tcgagagtcc cagtttcact
     3001 agcttttccg accatacgaa tgttgagaag gccaacgacc gtaccgataa gcttgctcaa
     3061 acggccgttc ttcaccaagt tatcgacttt ggctaggaca aagagcaact tagttttttc
     3121 ttgataggcg gtgatagctt caaccacttc ttcaaaagac aagccctggt caatcaagtc
     3181 attcaatttt tctacgagta ggtcaacttc accaccagca gataaactat caatcacatg
     3241 aatcttagtg tcaggatggt cttccagata aatattcttt gctagttgag cactattgtg
     3301 actgccagaa agggtacctg tgatggttac taggaaaatg tttttggcac cttcaaatgc
     3361 tcgcaaatag tcatctgggc ttggacaagc cgattttgaa gcttctgcag ttgcatacat
     3421 ggtttccatc atttggtcaa tatcgagact ggcgtcatca acaaagacct gatcagctac
     3481 ttgaatggtt aaggggacac ttacaaaggt tgtgttaata gctggtgttg gcagttgacg
     3541 ataatcacaa ccagagtcag caataatttt ccaagtcata gaaattctcc atctttgtca
     3601 gttatacatt gacaaaggtt ctgtcttttt ttacaattat atcatgaaaa cccttgaaac
     3661 aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat gttatgtctg aacgtagaat
     3721 ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa gaatccaatt tattaaccag
     3781 agaagccatt gaaacagccc tcttgcaact cttggaaaaa aaggagctga ccaagattag
     3841 tatttctgaa ttggtcaaac gtgcaggtgt ttcgcgtgcg gccttttatc gcaattatga
     3901 ctccaaagag gaaattttag agagcgtctt taaacgaact gtccacaata ttatggaaca
     3961 gctgcatcat tacgatttaa agacagacct ttatttggtt tgggttcacc ttttccggga
     4021 ggccagaaag gaagctagag tgattcaatt ggccttggat taccatctgg aaaaaatctt
     4081 tgtccaagcc atgcaggaat ttctagaaaa ataccatggg aaatcaaaag gtgtcagctc
     4141 ttatcttcat tccttctgga gctcagccat cgtctctgtc cttctaaaat ggatcaagga
     4201 tggcatgaag gtaccagctg aaaagattgc agatttaggt ttaccatttt ttaaaaaata
     4261 gagaaaaagg agaaaaaaga tgactgaaaa aagactggcc tgggatgagt attttgcagc
     4321 ccaagcccta ctaattgcga atcgttccac ttgtaaacgt gctaaagtgg gcgcgattct
     4381 ggtaaaagat aataaggtta tttccactgg ttacaatggt tcggtgtcag ggactgagca
     4441 ttgtattgat cacgaatgtt tgatcattga aggccactgt gttcgcaccc ttcacgctga
     4501 ggtcaatgct atccttcaag gtgctgagcg tggtgttccc aaaggcttta cagcctatgt
     4561 aacccatttt ccttgtctca actgtacaaa acaattgctt caggtcggct gcaagcgcgt
     4621 ggtttatatc aaccagtacc gaatggacga ctacgcccaa tacctttatc aagaaaaagg
     4681 gacagaattg acccatttac cacttgagac agtacagaca gctcttaaag aggctgatct
     4741 aatgtaaaaa ttatcaaaaa taaatggctt agaaagattt ttaaaccgtt ttttggtata
     4801 ataagaagaa taaattgaaa gaaggaattc caaaaatggg aaaaattgaa gttattaatc
     4861 acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac agatacttct acaaaagctt
     4921 ttcgtgagct agtagatgag attgcaatgt tgatggggta tgaagtactt cgtgatcttc
     4981 cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac agttcaaaaa caattggcag
     5041 gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat cgggatggtt gatggtctct
     5101 tgagcttggt tccagctgct aaagttggcc acatcggtat gtaccgtgat gaagaaacac
     5161 ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat tgaccaacgt caaattttta
     5221 tagtagaccc aatgttggca acaggtggct cagcaatctt ggctgttgat tctcttaaaa
     5281 aacgtggcgc atcaaatatc aaatttgtct gccttgtatc tgctccagag ggtgtaaaag
     5341 cccttcaaga agctcatcca gatgtagaaa tctttacagc agccttggat gaacgtttga
     5401 acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg agaccgcttg ttcggtacaa
     5461 aataagatcg aaaagaagaa tgacttggaa aagtatttcc agtcacgaaa ggaggttggg
     5521 tttttgtttc tgtctaatga aagcagagca aaaatttgac cttttttgac caagatatta
     5581 taatagtctt atcttcagtc atttgaccaa caaaattaaa actcaaaagg agaaatgaat
     5641 gattcctgta gttattgaac aaacaagccg tggagaacgt tcttacgata tttactcacg
     5701 tcttctcaaa gaccgcatca ttatgctgac aggtccggtt gaagacaata tggctaactc
     5761 tgttattgcc caattgcttt tcttggatgc ccaagatagt acaaaagata tttaccttta
     5821 tgtcaataca ccaggtggtt ctgtttcagc tggtttggca atcgtagata ccatgaactt
     5881 tatcaaggca gatgtccaaa ccatcgttat gggaatggct gcatctatgg gaacagtcat
     5941 cgcatcaagt ggagcaaaag gcaaacgttt catgcttcca aatgctgaat acatgattca
     6001 ccaaccaatg ggcggtacag gtggtggtac ccaacaaact gatatggcta tcgctgcaga
     6061 acacttgctc aaaactcgta ataccttgga aaaaatcttg gctgaaaatt caggtcagtc
     6121 aatggaaaaa gtccatgcag atgcagaacg tgataactgg atgagcgccc aggaaacact
     6181 tgaatatggc tttattgatg aaattatggc caacaattca ttgaactaat gatgatagaa
     6241 ggcaaactcg actgggtttg ctttttttgg tataataggg agagatttct tagaaagagg
     6301 atttatcatg tttgaaaaag tcaatcgctc tggcttgatt atctatcttt actataatcg
     6361 tgatgccaaa aaattgcagg attatggaga tattacctat cattccaaga aacatcgtta
     6421 cttacaactc tatgttccaa ctcaagaagt ggagcaattg gtcggacgct tgagcaagga
     6481 aaaatttatt aaaaaagtta gggtttgtca tatccaagag ttggaaacac cctttgtggg
     6541 caatctttat cgataggaaa acgttatcat agaaaaagtt caaggaaaac attgacaatt
     6601 ttctgataat tcggtatatt cttaacagac tatttaagaa ataaggagac aaaaagatga
     6661 agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt gcagcctgtg
     6721 gagaagtgaa gtctggagca gtcaacactg ctggtaactc agtagaggaa aagacaatta
     6781 aaatcgggtt taactttgaa gaatcaggtt ctttagctgc atacggaaca gctgaacaaa
     6841 aaggtgccca attggctgtt gatgaaatca atgccgcagg tggtatcgat ggaaaacaaa
     6901 tcgaagtaat cgataaagat aataagtctg aaacagctga ggctgcttca gttacaacta
     6961 accttgtaac ccaatctaaa gtatcagcag tcgtaggacc tgcgacatct ggtgcgactg
     7021 cagctgcggt agcgaatgct acaaaagcag gtgttccatt ggtctcacca agtgcgactc
     7081 aagatggatt gactaaaggt caagattacc tctttattgg aactttccaa gatagcttcc
     7141 aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa gttgttcttt
     7201 acactgacaa tgccagtgac tatgctaaag ggattgccaa atctttccgc gagtcataca
     7261 agggtgaaat cgttgcagat gaaactttcg tagcaggtga cacagacttc caagcagccc
     7321 ttacaaaaat gaaagggaga gactttgatg ctatcgttgt tcctggttac tataatgagg
     7381 ctggtaagat tgtaaaccaa gcgcgtggca tgggaattga caaaccaatc gttggtggtg
     7441 atggattcaa cggtgaggag tttgtacaac aagcaactgc tgaaaaagca tcaaacatct
     7501 actttatctc aggcttctca actactgtag aagtttcagc taaagctaaa gccttccttg
     7561 acgcttaccg tgctaagtac aatgaagagc cttcaacatt tgcagccttg gcttatgatt
     7621 cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa atcaagaata
     7681 accttgctaa aacaaaagat tttgaaggtg taactggtca aacaagcttc gatgcagacc
     7741 acaacacagt caaaactgct tacatgatga ccatgaacaa tggtaaagtt gaagcagcag
     7801 aagttgtaaa accataatag aaaaatgttg aaatagggaa tgagcctttg actcactccc
     7861 tgtttcgata tttaatactc ttcgaaaatc tcttcaaact gcgtcaacgt cgccttggat
     7921 tatatatgtg actgacttcg tcagtcttat ctacaacctc aaagcagtgc tttgagcaac
     7981 ctgcggctag tttcctagtt tgctctttga ttttcattga gtataagaac ctatcaaaaa
     8041 gtgagggaaa accctcggaa ttataaatag aaagagtgaa tcttatgctc caacaactcg
     8101 taaatggttt gattctaggt agtgtttacg cgctgttagc cctaggatat accatggttt
     8161 acggaattat caagctcatc aacttcgccc atggtgatat ttatatgatg ggagccttta
     8221 tcggttattt cttgatcaat tctttccaaa tgaatttctt tgtagcgctt attgtagcta
     8281 tgctagcgac agctattctt ggtgtcgtga ttgagtttct tgcttaccga cctttgcgcc
     8341 actctactcg tattgctgtt ttgattacgg ctattggggt ttctttccta ttggagtatg
     8401 gaatggtcta tctggttggt gccaataccc gtgccttccc tcaagcgatt caaacagttc
     8461 gatatgattt gggagcaatt agcttaacaa atgtgcagtt aatgattttg gccatttcct
     8521 tgattttgat gattttgtta caagtcattg tccaaaagac taagatgggg aaagccatgc
     8581 gtgcagtatc agtagatagc gacgcggcac aattgatggg gatcaatgta aaccgtacga
     8641 ttagctttac cttcgctttg ggttctgctc ttgcgggtgc ggctggtgtt ctgattgctc
     8701 tttattataa ctctcttgag cctttgatgg gggttactcc aggtcttaaa tctttcgttg
     8761 ccgcagtact tggtggtatc ggaattattc ccggtgcggc tcttggtggc tttgtgattg
     8821 gtctattgga aacctttgcg acagcctttg agatgtcaga tttccgtgat gccattgttt
     8881 atggaatctt gttgttgatc ttgattgtcc gcccagctgg tatccttggt aagaatgtga
     8941 aagagaaggt gtaaacgatg aaggaaaatt taaaagttaa tattctatgg ttactccttt
     9001 tgttagctgg ctatagcttg attagtgtac tggtttcagt cggagtactt aatctattct
     9061 atgtacagat tttacaacaa attggaatta atattatttt ggctgttggt ctcaacttaa
     9121 tcgttggttt ttcaggacaa ttttcacttg gtcatgctgg tttcatggcg attggtgcct
     9181 atgcagcagc tattattggt tctaaatcac caacctacgg tgccttcttt ggagctatgc
     9241 ttgtaggggc tttgctttca ggagcagttg ccttacttgt cggcattcca accttgcgct
     9301 tgaaggggga ctatcttgcg gtagcaactc tgggtgtttc tgaaattatc cgtatcttta
     9361 tcatcaatgg tggaagtctt acaaatggtg cggcaggtat cttagggatt cctaacttta
     9421 caacttggca aatggtttac ttctttgtcg tgattacaac cattgcaacc ttgaacttct
     9481 tgcgtagccc aattggccgt tcaaccctct ctgttcgtga agatgaaatc gctgctgagt
     9541 cagttggggt taatacgact aaaattaaaa tcatcgcttt tgtctttggt gccattactg
     9601 caagtattgc tgggtcactt caggcaggat ttatcgggtc tgttgtaccg aaagattaca
     9661 ccttcatcaa ctcaatcaac gttttgatta ttgttgtatt tggtggactc ggttccatta
     9721 caggtgcgat tgtttcggct attgttctgg gaattttgaa tatgcttctc caagatgttg
     9781 ctagtgtgcg tatgattatt tacgctttgg ccttggtatt ggtaatgatt ttcagaccag
     9841 gtggactcct tggaacgtgg gaactgagcc tatcacgttt ctttaaaaaa tctaagaagg
     9901 aggaacaaaa ctaatggcat tacttgaagt aaaacagtta accaaacatt ttggtggtct
     9961 aacagctgtt ggagatgtga ctcttgaatt gaacgaaggg gaactggttg gattaatcgg
    10021 tccaaacgga gctgggaaaa ccaccctttt caaccttttg accggtgttt atgaaccaag
    10081 cgagggaaca gtaaccctag atggtcacct tttgaatggg aaatcacctt ataagattgc
    10141 ctctttggga cttggacgta ctttccaaaa tatccgtctc tttaaagatt taacagtttt
    10201 agataatgtt ttgattgctt ttggaaacca tcacaaacag catgttttta ctagtttctt
    10261 acgcttacca gctttttaca agagtgaaaa agaattaaag gataaagctt tggaattgtt
    10321 gaaaatcttt gatttagatg gtgatgcaga gactcttgct aaaaatcttt cctacggaca
    10381 acaacgtcgt ttggaaattg ttcgtgccct tgctacggaa cctaaaattc tcttcttaga
    10441 tgaaccagca gcaggtatga acccacagga aacagccgaa ttgactgagt taattcgtcg
    10501 tatcaaagat gaatttaaga ttacaatcat gttgattgaa cacgatatga atctggtcat
    10561 ggaagtaaca gaacgtatct acgtacttga atatggccgt ttaattgctc aaggaactcc
    10621 agacgaaatt aagaccaata aacgcgttat cgaagcttat ctaggaggtg aagcctaatg
    10681 tctatgttaa aagttgaaaa tctttctgtg cattacggta tgatccaagc agtccgtgat
    10741 gtaagctttg aagttaatga aggagaagtt gtttccctta tcggtgccaa cggtgcaggt
    10801 aagacaacta ttcttcgcac cttgtcaggt ttggttcgac caagttcagg aaagattgaa
    10861 tttttaggtc aagaaatcca aaaaatgcca gctcagaaaa tcgtggcagg tggtctttca
    10921 caagttccag aaggacgcca cgtctttcct ggcttgactg ttatggaaaa tcttgaaatg
    10981 ggagctttct taaagaaaaa tcgtgaagaa aatcaagcta acttgaagaa ggttttctca
    11041 cgctttcctc gtcttgaaga acggaagaac caagatgcag ctactctttc aggaggggaa
    11101 caacaaatgc ttgccatggg acgcgctctt atgtcaacac caaaacttct tcttttagat
    11161 gaaccatcaa tgggacttgc cccaatcttc atccaagaga tttttgatat cattcaagat
    11221 attcagaagc aaggaacaac cgtcctcttg attgaacaaa atgccaataa agcacttgca
    11281 atctctgacc gaggatatgt attggaaaca ggaaaaatcg tcctatcagg aacaggaaaa
    11341 gaactcgctt catcagaaga agtcagaaaa gcatatctag gtggctaaaa caatccagtg
    11401 gattgtttta gtcggcagat gaagattacg aagtaatcat caatatagtc cgggggccct
    11461 ttttagtcgg tggatgaaga ttgcgaagca atcatcaaat ccagaggatt gttttagccg
    11521 gtagattgag attgcaaaca aatctgcatc tacattgaaa gctgaatttc taataattga
    11581 aaaaatcgaa tgaaacgtat cttaccttca ttcagggagc tcgattttag agctcttttt
    11641 gctagcttat tcatactttt ttgaatttcg aaaaagaaat gtaagcgttt gatagattta
    11701 caaaaagatt gtataatggg gataagaata gaaaaggaga agtctcatgg cagttaaaga
    11761 ttttatgacc cgcaaggtag tttatattag tccagataca acagtatctc atgcagcaga
    11821 tttgatgaga gagcaagaat tgcaccgctt gcctgttatc gaaaatgatc aattggttgg
    11881 tttggtgact gagggaacca ttgcacaagc aagtccatct aaagcaacaa gtctttctat
    11941 ctatgagatg aattatcttc tgaataagac aaaagtaaaa gatgtcatga ttcgcgatgt
    12001 tgtcactgtc tcaggctatg ctagtctaga agatgcaact tatctgatgt tgaaaaataa
    12061 gattggtatt ctccctgtcg tagataacca tcaagtatac ggagttatta ctgaccgtga
    12121 cgttttccaa gcctttcttg aaattgcagg ttatggcgaa gaagggattc gtgtacgctt
    12181 tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt tctttgattg tagaagaaaa
    12241 tttgaatatc tcccatacag tcaatattcc gcgtaaggat ggtaaggtca ttatcgaagt
    12301 gcaaatcgat ggatcaattg atttaccagc cttgaaagaa aaatttgaag caaatggtat
    12361 tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg taagaaggga agcccaaagg
    12421 cttctttttt catgaaaagg ggattagaga aaaagatgga aagaaatgat aaaatatgct
    12481 ataatgaaat aatgtaaaaa aggagtattt atggacattt cagtaattcg tcagaaaatt
    12541 gacgcaaatc gtgaaaaatt agcttctttc agggggtctc tttgacctcg aagggctaga
    12601 ggaagagatt gccatcttgg aaaacaagat gacagaacct gatttttgga acgataatat
    12661 tgcggcccaa aaaacgtcgc aagaattaaa tgaattaaaa aacacttaca ataccttcca
    12721 taagatggaa gagttgcagg atgaagtcga aattttattg gattttttgg ctgaagacga
    12781 gtcagtgcat gatgaactgg tagcgcagtt agccgaactt gataagataa tgaccagcta
    12841 cgagatgact ctactcttgt cagaacctta tgaccacaac aatgccatct tggaaatcca
    12901 tccaggttct ggtggtactg aggcgcagga ctggggtgat atgttgcttc gtatgtatac
    12961 tcgttatggt aatgctaaag gctttaaagt ggaagtgttg gattaccaag caggtgatga
    13021 ggctggtatt aagtcggtaa ctttatcatt tgaagggcct aatgcctatg gtctcctcaa
    13081 gtcagaaatg ggtgttcacc gcttagtgcg aatctcacca tttgactctg ccaaacgtcg
    13141 ccatacctct ttcacatctg tagaagtgat gccagaattg gatgatacta ttgaagtgga
    13201 aatccgtgaa gatgatatca agatggatac cttccgttca ggtggtgccg gtggacaaaa
    13261 cgtcaataag gtttcaacag gtgtacgttt aacccacatt ccaactggaa ttgttgtcca
    13321 atcaacagtg gatcgtaccc agtatggaaa tagagatcgt gccatgaaga tgttgcaggc
    13381 taagctctat caaatggagc aagataagaa ggctgcggag gtagattctc tcaaaggtga
    13441 gaaaaaggag atcacttggg gaagccaaat ccgttcttat gtcttcacgc cttatactat
    13501 ggtaaaagat caccgaacta gctttgaggt tgctcaggta gataaggtta tggatgggga
    13561 cctagatggt tttatcgatg cttatctcaa gtggcgaatt agctaagata gaaaggaact
    13621 cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat acgacaacgg aacaactgct
    13681 ctacgcggtg tttcggttag cgttcaaccg ggggaatttg cttacatcgt aggaccttca
    13741 ggagcaggga agtcaacttt tattcgttct ctgtatcgtg aagtaaaaat cgataaagga
    13801 agcctatcag ttgctggttt taatctggtt aagatcaaaa agaaagatgt cccgcttcta
    13861 cgtcgtagtg ttggggttgt cttccaggat tataaactgt taccaaagaa aactgtctat
    13921 gaaaatattg cttacgctat ggaagtaatc ggggaaaatc gccgtaatat caaaagacga
    13981 gtgatggaag ttttggactt ggttggattg aagcataagg ttcgttcttt tccaaatgaa
    14041 ctctcaggtg gggagcaaca gcgaattgcg attgcgcgtg caattgtaaa taatcccaaa
    14101 gtattgatag ctgatgagcc aacaggaaat ctggatccgg ataattcatg ggaaattatg
    14161 aatctcttgg aacggattaa cctacaagga acaactattt tgatggcgac tcataatagc
    14221 cagattgtaa ataccttgcg ccaccgtgtc attgccattg aaaatggccg tgtcgttcgt
    14281 gacgaatcaa aaggagagta tggatacgat gattagtaga ttttttcgcc atttatttga
    14341 agccttaaaa agtttgaaac gaaatggttg gatgacagta gctgctgtca gttcagtcat
    14401 gattactttg accttggtgg caatatttgc atctgttatt ttcaatacag cgaaactagc
    14461 tacagatatt gaaaataatg tccgtgtagt agtttatatc cgaaaggatg tggaagataa
    14521 tagtcagaca attgaaaaag aaggtcaaac tgttacaaat aatgactacc acaaggtata
    14581 tgattctttg aagaacatgt ctacggttaa aagtgttacc ttttcaagta aagaagaaca
    14641 atatgaaaaa ttaaccgaga taatgggaga taactggaaa atctttgaag gagatgccaa
    14701 tcctctctat gatgcctata ttgtagaggc aaacactcca aatgatgtaa aaactatagc
    14761 cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa gatggcggtg ccaatacaga
    14821 aagactcttc aagttagctt catttatccg tgtttgggga ctagggattg ctgctttgtt
    14881 aatttttatc gcagtttttt tgatttcaaa taccattcgt attaccatta tttcccgcag
    14941 tcgcgaaatt caaatcatgc gcttggtcgg agctaaaaac agttatatcc gtggaccgtt
    15001 cttgttagaa ggagccttta tcggtttatt gggagctatc gcaccatctg ttttggtctt
    15061 tattgtttat caaattgttt atcaatctgt caacaaatcg ttggtagggc aaaatctatc
    15121 catgattagt ccagatttat ttagtccgtt gatgattgcc ctactatttg tgattggggt
    15181 tttcattggt tcattgggat caggaatatc catgcgccga ttcttgaaga tttaggtaaa
    15241 atagctgctt ttatgaggag attgtaaaat ctcctttttt gctacaagag tttttgaaaa
    15301 gagatgcgca gaagaaaaga gcttccaaag aagtccccca gagaagactt cttgatggct
    15361 aatttgaact tgaaatttga ctcactagaa attttttaga aaaaagtgta atttttttat
    15421 aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat tatagaaggt gcaaacgttt
    15481 tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag atacattcaa
    15541 aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg tagttatcgc
    15601 ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga tgattaaccc
    15661 aacctttaca ccacttgtca tcacaggtgg aattcttgag caaatcggtt ggggggttat
    15721 cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta aagaacgtgc
    15781 tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca ctggtacaat
    15841 ttttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta ctttctttgg
    15901 tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag ccttgaacat
    15961 gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca acaaatacta
    16021 caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt tcgtaccatt
    16081 tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct ggccagtagt
    16141 tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa ctgctccaat
    16201 tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg gtcttcacca
    16261 catgttgact atcccaatga actacacagc tcttggtggt acttatgaca ttttaactgg
    16321 tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat gggtaacaga
    16381 ccttgtaaac cttaaaggta ctgatgctag tcaatatcaa cacttgttag atacagtaca
    16441 tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga tgggtgtgat
    16501 tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag gtatgatgat
    16561 tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat acatgttcat
    16621 gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg ccttcgctat
    16681 ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct tgactcgtac
    16741 acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt gggtaactgt
    16801 tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat tcaactacgc
    16861 aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa ccagcagcga
    16921 agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg gtggacgtgt
    16981 aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta aagatgcaga
    17041 taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg tgatgaaagg
    17101 acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg atatccaaga
    17161 tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga ctgaagcaca
    17221 acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag cagacggtca
    17281 agttgttggt ttggaacaag taaaggatcc agtatttgct caaaaaatga tgggtgatgg
    17341 atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta ctgtgtcaag
    17401 catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg aagtattggt
    17461 tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc atgttgctga
    17521 aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg ctatccgtgc
    17581 agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa ttaaatcagt
    17641 taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag tagaattgta
    17701 atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa gaatgaaatt
    17761 tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat tccagatttt
    17821 gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa tcaatcagga
    17881 gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag cagctgagcc
    17941 tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc aagggaaaaa
    18001 ttactactgg acctgggctt ataaccatat cggctatgac cgctaccacg aaggtgtggc
    18061 tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg tggatgatcc
    18121 aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg gcaaggagct
    18181 agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag aatgggcacg
    18241 atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag attttaacaa
    18301 cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct tacaagacgc
    18361 atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa ttgatggctg
    18421 gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag agttagcggt
    18481 ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg atcactatgg
    18541 cttgaatgct atgttaaact ggaaataata actgaaaaga ggttggaact ataaaattcc
    18601 agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac tactgtaatg
    18661 gaataaaata tggtataatt gataagatag atagaatcga ggatgttatg tcatttacga
    18721 aatttcaatt taaaaactat attagagaag ccttggagga gttaaaattt acaactccaa
    18781 cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta gtaggagaat
    18841 caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag caattagatg
    18901 aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg gctactcaaa
    18961 tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt gtggttaatt
    19021 atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggaaagcaat cagcctcata
    19081 ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat ttagctattc
    19141 ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg ggattcttgg
    19201 aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg gtcttctcag
    19261 cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat cctgttatgg
    19321 agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg atttcgacca
    19381 agggacatga taagaatgct caaatttacc agttgactca gttgatgcag ccgtatttgg
    19441 caatgatttt tgttaacact aaaacgcgtg ctgatgaatt gcattcatat ctgactgctc
    19501 aaggcttgaa ggttgcaaaa atccatggcg atattgcccc tcgtgaacgc aagcgaatca
    19561 tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg gcagcacgtg
    19621 ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa gacttatctt
    19681 tttttgttca ccgtgttggt cgtactggac gaaatggcct accaggtaca gctattaccc
    19741 tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga atcaagttta
    19801 gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat cgtcgtgcca
    19861 accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt aaaaagaaaa
    19921 agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat gaaaagcgcc
    19981 gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa gctaaacgcc
    20041 aaacatttta atagaaattg ttggagtact gagctccaac ttttttattt atgagaacga
    20101 actatctaaa tcgaaacact acattaaaga ctgcaaattg cgattaaaaa tggtataatg
    20161 ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt ttcctatcag
    20221 tactttgtaa ctctataaca atatttttta aggggggaca tttttatgtc agaacgtaaa
    20281 ttattcacgt ctgaatctgt atctgagggg catccggata agattgcaga ccaaatttca
    20341 gatgcgattt tggatgctat tttagcaaag gatccagagg cgcacgttgc tgctgaaaca
    20401 gctgtatata ctggttctgt ccacgttttt ggtgaaattt ctacaaatgc ctatgtggat
    20461 attaaccgtg tggttcgtga taccattgca gagattggtt ataccaatac agaatatgga
    20521 ttttctgctg agacggtggg agtacaccca tctttggtgg aacaatctcc tgacatcgct
    20581 caaggtgtta acgaagcctt ggaggttcgt ggaaatgctg atcaagatcc actggacttg
    20641 attggagcag gtgaccaagg gctcatgttt ggatttgcag tagatgaaac agaagagctc
    20701 atgccattgc caattgcact cagtcataaa ttggttcgtc gtctggcaga acttcgtaag
    20761 tctggagaaa ttagctatct ccgtccagat gcaaaatcac aagttacagt tgagtacgat
    20821 gaaaatgacc gtccggtacg tgtagataca gtcgttattt ctactcagca tgatccagag
    20881 gccactaatg aacaaatcca tcaagatgtg attgacaagg tcatcaaaga agttattcca
    20941 tcttcttatc ttgatgataa gacaaaattc tttatcaatc cgacaggtcg ttttgtaatc
    21001 ggtggtcctc aaggggactc aggtttgact ggtcgtaaga ttattgtaga tacttatggt
    21061 ggctactctc gtcatggtgg tggtgccttc tctggtaaag atgcgactaa ggtggatcgt
    21121 tcagcttctt atgcggctcg ctatattgcc aagaatatcg ttgcagcagg ccttgctaag
    21181 aaggcagaag tgcagttggc ctatgctatc ggtgttgcgc aacctgtttc tgttcgtatc
    21241 gatactttcg gtacaggaac agtagctgaa agtcaacttg aaaaagcggc tcgtcaaatc
    21301 tttgaccttc gccctgcagg gattatccaa atgctgaacc ttaagcgtcc aatttaccgt
    21361 caaacatcgg cttacggtca catgggacgt acagatattg atcttccatg ggaacgtttg
    21421 gataaggtag atgctttgaa agaagcagta aaataagatt ttaagagggg aacgtcctct
    21481 cttttttata gtttttaact atactgggat actattctga aaatccattt tgcgaaagta
    21541 gagatttaca tgtatagtag attgaaacta gaatagtaca cctcaacttc taaaacattg
    21601 ttagaaatcg atttgactgt cctgatcgat ttctcctgtt cttgtttcat tttactatat
    21661 ttctctaaaa atgataaagg ttgagatttc tcctcgtaat agataatcat ggggatattt
    21721 caatccaaag ttttattcgt tatcacttga ctattgcaag gttttctaga gcaacagagt
    21781 catggaatgg actcatggtt gagatttctc cttgttgctt ggacttcatt caaaagtctg
    21841 ttacccaagc cttgttcaaa cttctaatac actagctgtt tccatagcat gacttctgta
    21901 ctagactttc ttttccgaat aaatagatag aaccacagaa tctagtaaac ctagaattaa
    21961 aattatggta taatattagc aataaaagaa atctggagga ttagaatcat ggtatcaacg
    22021 aaaacacaaa ttgctggttt tgagtttgac aattgcttga tgaatgcagc aggtgtggct
    22081 tgtatgacga tagaggagtt agaagaggtc aaaaactcag cggcaggaac ctttgttact
    22141 aagacagcga ccttggactt ccgtcagggg aatcctgagc cacgctacca agatgttcca
    22201 cttggttcca tcaactctat gggcttgcca aataatggct tagactatta tttggattat
    22261 cttttagatt tgcaggaaaa agagtcgaac cgaactttct tcttatctct ggtcggcatg
    22321 tctccagagg aaacccatac tattttgaaa aaagtccaag agagtgattt tcgtggtctg
    22381 actgagctaa atctttcctg tccaaatgtt ccaggtaaac ctcagattgc ctatgatttt
    22441 gagacaacag accggatttt ggcagaagtg tttgcttact tcaccaaacc tcttggaatt
    22501 aaattgccac cttattttga tattgttcac tttgatcaag cggcagctat tttcaacaaa
    22561 tatccgctca agtttgtcaa ctgcgttaac tctatcggaa acggcctcta tatagaagac
    22621 gaatctgtcg ttattcggcc taagaatggt tttggtggaa ttggtggaga atacatcaaa
    22681 ccgactgctc tagccaatgt tcacgccttt tatcaacgtt taaatcctca aatccaaatt
    22741 atcggaacag gtggcgttct gactggtcga gatgcctttg aacacatcct ctgtggagca
    22801 agtatggtgc aggtgggaac gacccttcac aaagaaggcg tcagtgcttt tgaccgcatt
    22861 accaatgaac tgaaagcaat catggtggaa aaaggctacg agagcttaga agatttccgt
    22921 gggaaattgc gctatattga ctaaattaaa tcgaaaaatc tgaagaaagg agagacgatg
    22981 ctagccattg aagaaagtca gaagttgact ttatcaaatt taccgagcct gagcctattt
    23041 acagggacag atcagggtca gtttgaagtg atgaagagtc aagtgttgaa acagattggg
    23101 tatgattctg ctgacctcaa ctttgcctac tttgatatga aagaagtagt ttacaaggat
    23161 gtggaactgg agttggtcag ccttcctttc tttgcggatg agaaaatcgt gatattagat
    23221 tattttatgg atatcacgac tgctaagaaa cgctttttga cagatgatga gcttaagtca
    23281 tttgaggaat accttgacaa tccttctcca acaaccaagt tgataatctt tgcagaagga
    23341 aagctggata gcaaaagacg gttagtcaaa ttacttaagc gtgatgccaa ggtcttcgat
    23401 gcagtagaag taaaagaaca agaattgcgc cagtacttcc aaaagtggag tcagaaacaa
    23461 ggtctgcagt ttaccaatca ttcttttgaa aatctcctca tcaagtcggg gtttcaattt
    23521 agcgaaatcc agaaaaatct tctcttttta cagtcctata aggtggattc tgttatcgag
    23581 gaagaggata ttgttaacgc aattcccaag accttgcagg acaatatttt tgatttaact
    23641 cagtttattc tgactaaaaa gatggatcag gcgcgcgatt tggtgagaga cttgaccttg
    23701 caaggggaag atgaaatcaa actgattgcg gtcatgttgg gacaatttcg gacttttact
    23761 caggtgaaga ttttggcgga gtctggccaa acagaatcgc agattgcaag tagtttaggt
    23821 agttatctgg gacgtaaccc aaatccttat caaatcaagt ttgcattaag agattcgaga
    23881 ggactttctt tgagctttct gaagcaagct atttcctatt tgattgagac agactatcag
    23941 attaagacag gtctttatga aaaaggtttc ctttttgaaa aggcactctt acagattgct
    24001 agtcaggtca attgacattt gttgaaacta caacccgcgt caggattatc ttgtcgtggg
    24061 tttcttgctg atttgcttat tttattagaa agtatgtcag tgctttcctt atctataagc
    24121 aaaaaacttg aaaaaagtga atgtttgcgt tatcattttc atatagaaag aaaaagaggt
    24181 attacagatg gctattatct taccagaact tccatatgca tacgacgctt tggaaccata
    24241 catcgatgcg gaaacaatgc atttgcacca tgacaaacac catcaaactt atgtcaacaa
    24301 tgccaatgca gctttagaaa aacaccctga aatcggtgaa gaccttgaag ccttgcttgc
    24361 tgatgtagaa tctatcccag ctgatatccg tcaagcactt atcaacaatg gtggaggaca
    24421 cttgaaccac gctcttttct gggaattgat gactcccgag aaaacagctc cttcagcaga
    24481 actggcagca gcaatcgatg caacatttgg ttcatttgaa gaattccaag cagccttcac
    24541 tgcagcagca acaactcgtt ttggttcagg ttgggcatgg ttggttgtca acaaagaagg
    24601 gaaacttgaa gtgacttcaa cagcaaacca agacacacca atctcagaag gtaaaaaacc
    24661 aatcttgggc ttggacgttt gggaacatgc ttactacgtg aaataccgca acgtgcgtcc
    24721 tgactacatc aaagctttct tttcagtaat caactggaat aaagtagatg aattgtacgc
    24781 agctgctaaa taatgatagt tggagggaag aattgttctt ctctttttta ggttataaga
    24841 ttctggtctg acaaaatcgt cagacttttt tcatttttat gagaaacgtg ctaactgcta
    24901 gaaattatgg tagaataaag tattaagtaa tcgtggaaaa aggatatcta tgcgaaaaga
    24961 aattgcacct gaattataca actataacaa gtttcctggt ccggagttcc atttacacgg
    25021 ggacaaggtc gaaacggaag ggatagcttt ttccttggtt gaaaatatca aggatgcctt
    25081 tgatgtgacg acttttaatc agcgtttttc agaagtctta accaagtttg attatatcgt
    25141 gggggactgg agcaacgaac agcttcgcct acgaggtttt tacaaggatg accgaacaga
    25201 agaaaaactt gaaaaaatca gtcgtttaca agactacctt ttagagtatt gtagttatgg
    25261 ttgtgcctat tttgtcttag aaaatgaagc ccctaagcga gcatcatttg acaagaaaat
    25321 gcgtaagacg gaagaagaaa caccttctag aaaaggaaag aaaccgactc aaactaaacg
    25381 aaagtcgaat gcagataaga aaaatagacg tcgtcagaaa gaccagcatt ctcagaaaga
    25441 ggacaaggga caacgtcatt ttgtcattcg tcagaagtga gtagagaata aggagaagaa
    25501 atgaaaccct caattcatag tttgccacat caaaccatgc aggaatgggt attggagcag
    25561 ggagaaaaga aattccgtgc agatcaaatc tgggaatggc tctaccgtaa acgtgtgcag
    25621 tcatttgaag aaatgaccaa cctttccaag gatttgattg ctaagctcaa tgaccagttt
    25681 gtggtcaatc ccttgaaaca acgtattgtg caagagtctg ctgatggtac agtcaaatat
    25741 ctctttgaat tgcccgacgg tatgttaatt gagactgtac tcatgcgtca acactatggt
    25801 ttatctgtct gtgtgaccac tcaggtcggc tgtaatatcg gttgtacctt ctgtgcctct
    25861 ggtttgatta agaagcagcg tgacctcaat aacggtgaaa tcgtagcgca aatcatgctg
    25921 gttcagaaat actttgatga gcgtggtcag gatgaacgca tcagccatat cgttgtcatg
    25981 ggaatcggtg agccctttga taattacaac aatgtcttga atttcgtttg tactatcaat
    26041 gatgataaag gaatggctat cggtgctcgt cacatcacga tttcaacctc aggtttggct
    26101 cataaaattc gtgactttgc tgatgaaggg gttcaggtca atcttgccgt gtctcttcac
    26161 gcacccaaca atgaacttcg ttcaagtatc atgaaaatta accgtgcctt tccgattgaa
    26221 aaactctttg ctgctattga atactatatt gagacaacaa accgtcgtgt aacctttgaa
    26281 tatatcatgc ttaatgaagt caacgacggt gtagaacaag ccttggaatt ggctgaattg
    26341 ctcaagaaca tcaagaaatt gtcttatgta aacttgattc cttataaccc agttagtgag
    26401 catgaccaat atagccgtag tcccaaagag cgcgtgttgg ctttctatga tacgcttaag
    26461 aaaaaagggg tcaactgtgt ggttcgtcaa gagcatggta cagatattga cgcagcttgt
    26521 ggacaattgc gctctaacac aatgaaacgt gaccgccaga aggcagtcgc agcggtaaat
    26581 ccttaatgat gaagaagact tataatcata ttttggtctg gggagtcatt ttctatagca
    26641 tttgcattgt ctatttttgc tttactcctc aagaacaatc taccgtgaga gtgggaactc
    26701 caggtattca gcatcttgga cgcctggttt ttcttttgac tcctttcaat tctctctgga
    26761 aactgggcga agtgagtgac attggacaat tatgttggat ttttttacaa aatgtcctca
    26821 atgtcttctt attttttcct ctgattttcc aactccttta tctatttcca aatttgcgga
    26881 aaacaaaaaa ggtccttctt tttagttttc ttgtgagtct tggaatcgag tgtacgcaat
    26941 taatcttgga ctttttcttt gatttcaatc gcgtctttga gattgatgat ttgtggacca
    27001 atactttggg tggctatctg gcttggctcc tttataaacg attacataaa aacaagataa
    27061 ggaattaaaa tgagtatttt agaagttaaa aatctgagtc acggttttgg tgaccgtgca
    27121 atttttgaag atgtgtcctt ccgtctcctc aagggagaac atatcggcct ggtcggtgcc
    27181 aatggtgaag gaaaatcaac ctttatgagt atcgtgactg gtaaaatgct gccagatgaa
    27241 ggaaaggttg agtggtccaa atatgtgacg gctggttact tggatcagca ctctgtcctt
    27301 gctgaaagac agtcggtgcg tgatgttctc cgtacggctt ttgatgagct tttcaaagct
    27361 gaagctcgta tcaatgacct ctatatgaaa atggctgaag acggcgcgga tgttgatgct
    27421 ctcatggaag aagtaggaga acttcaagac cgtctggaga gtcgtgattt ctataccttg
    27481 gatgctaaga ttgacgaagt agcgcgtgct cttggtgtta tggactttgg catggatacg
    27541 gatgtaactt ctttgtcagg tgggcaaaga accaaggtgc ttttggcaaa acttctcctt
    27601 gaaaagcctg atattttgct gttggacgag ccgaccaact acttggatgc tgagcatatt
    27661 gattggctca agcgctatct ccaaaactat gagaatgcct ttgttctcat ttcgcacgat
    27721 attccattcc tcaatgacgt tatcaatatt gtctatcatg tggaaaatca acagctgacg
    27781 cgttactctg gtgactacta ccagttccaa gaagtttatg ctatgaagaa atctcagcta
    27841 gaggcagcct acgaacgcca gcagaaagag attgcagacc tcaaggactt tgtggctcgt
    27901 aataaagccc gtgttgcaac tcgtaatatg gctatgtctc gtcaaaagaa attggataag
    27961 atggatatta tcgaactcca aagtgagaaa ccaaaaccat cctttgattt caaaccagct
    28021 cgtacaccag ggcgctttat cttccaagcc aagaacttgc aaattggtta cgaccgtcct
    28081 cttactaagc ctttaaatct taccttcgaa cgcaatcaaa aggttgcgat tattggtgct
    28141 aatggtattg gaaaaacaac tctcttgaag agtctcttag gcattatctc gccaatcgct
    28201 ggggaagtgg agcgtggaga ttatttagaa cttggttatt ttgagcagga agtagaaggc
    28261 ggtaatcgcc aaactcctct tgaagctgtc tggaatgcct ttcctgccct taatcaagca
    28321 gaagtccgtg cagcccttgc ccgttgtggt ttgacaacca aacatattga aagccagatt
    28381 caagtattat cagggggaga gcaagccaag gttcgtttct gtctcttgat gaatcgtgaa
    28441 aacaacgttt tagtgctgga cgagccgacc aaccatttgg atgtggatgc aaaggatgag
    28501 ctcaaacgcg ctctcaaaga atacagggga tctatcctta tggtctgcca cgagccagac
    28561 ttttatgaag gttggataga ccaaatatgg gattttaata atttaactta agaatgagag
    28621 taaaagaaat acagtaccga atgtggatac tgtatttttt tggtttttaa gatttaaaat
    28681 cgtagtcttt caccacttcg atgctgtcga ttgtgatagc agtagttggc ttgtcttttt
    28741 catctttttc ggctttagca atcttatcca caacatccat accgtcaatc acttgaccaa
    28801 agactgggtg tttgccatct agactagggt ttccaccttc tttgtaggct tcgataattt
    28861 tctgtggata cttgcttgta gggagtttag aagaggtatc tgtagagttt tggttgatga
    28921 agaactggct gccattggtg tttggttgac cagtattagc catagcaaga gcaccacgga
    28981 tgttatacaa ataaggagta atctcgttct tgaaaccagt tcctttgtct ttagtcttat
    29041 ccttgtcatg ccatatggac tgaccacctg taccgtcccc ttttggatct ccagtttgga
    29101 ccataaagcc atcgatgaca cggtggaagg taataccgtt atagtagcct tctttggcgt
    29161 gagtgaggaa attttcaacc gctagaggag cgagtttagg gaagagttta atgcgaatat
    29221 caccttggct tgtgtggaga atcacttcgg cttcatcttc agcaacttcc ttagatagtt
    29281 gtgggaaatt ggcgttttcg tttgttaaag catcgtttaa ctccttggca gattgggcag
    29341 ctactttgga actttcttca gcagcaagac tggaatcaac ataatcatca ccacgcagac
    29401 tgcgttggac gctgctacac ccagctaggg ctacagtaga cagtaaaaga agggttgcta
    29461 gttttttcat tctgatcctc tcaaaaattc atttatacca ttgtacccta aaaggaagtg
    29521 gatttcaaat attagtagtt tacagaactg gcttagataa tagttgggat ttcatcatca
    29581 tcttgaaaat cttcaacttc ttcaacttct tccccagtaa atggtttctg aaaagaactt
    29641 agtataaaac gggcaattgg ttcaacaatt aggaaattta gaggaagagc tgcaataaag
    29701 tttacgaacc aggcgcttag ataggtattg ggtgtgatag caatgccatt gtaaaaaagt
    29761 ccatagaagg acatgaaagt gaccagctaa ttcttcttct gaaaaatgct catgaagggc
    29821 ttgatccagg tcgcaagatt tcattttaga aagtccagta actcccttct tcttcaggat
    29881 attctttttc atggtattat ttagatggtc aagtgagtca aaagaagttt cgacaagagc
    29941 ataccctttt tctaccagag tttttagatg tttaggggca tcaataccat aagtgtactc
    30001 aaagtatttt ggaaaccaga tcttaagaaa gctcgtaaag ctagccagtt ctctaaacgc
    30061 taagaactag ctacaacaca gcatttacaa cacttcatgg ttcacaccat ggggtgtttt
    30121 ttaatgattt taagcaaaat tcacacttaa ttgaaagtta tttaaattag atgaaaattt
    30181 attttttgaa aaagtgggaa aacattgatt ctaagcgaat tttgaatcat attcgaatgg
    30241 gagtttttaa aatcatgttt cagtggtaaa agtaccaaac tggattctcc atagtaaaat
    30301 gatatctcct gctagtgaat catctgctaa taagtccata tttcgcttgg gacttagaag
    30361 ccaagtatcc aactctcgtt caggcgatat gaagggtttg gcttggtgtt ctttataaaa
    30421 tttttctaaa atagtattca cggatagcat ctttcaaaca tttatttcct attctaacaa
    30481 atttctaaat gctttaagaa atttatagtc tgtcaagttt ttttcttgac acctgccaga
    30541 aatctgctat aatagaacat gtgctaaata gctcagctat ttcaccgaaa taaaaaatta
    30601 aagaaatgag actttaaaaa tggcagttaa aatccgtttg actcgtatgg gttctaagaa
    30661 aaaacctttc taccgtatca acgtagcaga ttcacgttca ccacgtgacg gacgtttcat
    30721 cgaaacagtt ggaacttaca acccacttgt tgctgaaaac caagtaactt tgaaagaaga
    30781 ccgcgttctt gcatggttgg ctaatggagc tcaaccttca gacacagtac gtaacatcct
    30841 ttcaaaagaa ggcgtattga aaaaattcca cgattctaaa ttctcaaaat aagtttaaag
    30901 taggttgaca gatggatacg attgaaaatc tcattattgc gattgtgaaa cccttaattt
    30961 cacaaccaga tgccttaact atcaagattg aggatacacc agaatttttg gaatatcatt
    31021 tgaatcttga tcaaagcgat gtgggtcgtg taatcggtcg taagggtcgc actatttctg
    31081 cgataagaac gattgtctac tctgtcccaa ctgaatacaa aaaagtaaga atcgttattg
    31141 acgaaaaata agaagggcgg gacggatgtc tcgctttttt gtgtggaata gaagatatag
    31201 taaaatgaaa taataacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt
    31261 tttagaagta aaggtgtact attctagttt caatatacta taaaagattg aacgtttagg
    31321 caattccaag actacttaat cgaacattaa cagcagtctc ggactgatga aggtcttttt
    31381 atgaagttag tggaggaagt cggagaagta gccgaggttt tgaataggcg ctctggtcga
    31441 aaagaaggga taggattcga acgaggaact tgccagagaa ctggctgata tcatttacta
    31501 caccgtcgca atcgtagcta ttaatcatat tgaccttacc aagactgtct ttgagaaaga
    31561 caaaaccgca gccgttaatt accaacataa acataatttg gaaggattat tgaagggaaa
    31621 gtagagatat tgtccccttc ctccaacttg agaaaatcct tggaacgtga taaaataaag
    31681 gataaggat
//
LOCUS       DAVKZB010000012        31416 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKZB010000012 DAVKZB010000000
VERSION     DAVKZB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.012"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            640..1916
                     /gene="ltrA"
                     /locus_tag="VXA80_000498"
                     /pseudo
     CDS             640..1916
                     /gene="ltrA"
                     /locus_tag="VXA80_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2372..3082
                     /locus_tag="VXA80_000499"
     CDS             2372..3082
                     /locus_tag="VXA80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0353043.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSSLSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLLYQQI
                     YWKFFQIRFGFLPLYLFL"
     gene            3028..3549
                     /locus_tag="VXA80_000500"
     CDS             3028..3549
                     /locus_tag="VXA80_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0353044.1"
                     /translation="MEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTVYSIGIIISG
                     LIAFRLSEKFLAAKWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVP
                     ESVFLQETVEENHLVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMI
                     EAILIYIRRDYFK"
     gene            3863..3994
                     /locus_tag="VXA80_000501"
     CDS             3863..3994
                     /locus_tag="VXA80_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV0353045.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4015..5565
                     /gene="dltA"
                     /locus_tag="VXA80_000502"
     CDS             4015..5565
                     /gene="dltA"
                     /locus_tag="VXA80_000502"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV0353046.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5562..6806
                     /gene="dltB"
                     /locus_tag="VXA80_000503"
     CDS             5562..6806
                     /gene="dltB"
                     /locus_tag="VXA80_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV0353047.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6820..7059
                     /gene="dltC"
                     /locus_tag="VXA80_000504"
     CDS             6820..7059
                     /gene="dltC"
                     /locus_tag="VXA80_000504"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV0353048.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7052..8320
                     /gene="dltD"
                     /locus_tag="VXA80_000505"
     CDS             7052..8320
                     /gene="dltD"
                     /locus_tag="VXA80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV0353049.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8475..8915
                     /gene="adcR"
                     /locus_tag="VXA80_000506"
     CDS             8475..8915
                     /gene="adcR"
                     /locus_tag="VXA80_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV0353050.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8915..9619
                     /locus_tag="VXA80_000507"
     CDS             8915..9619
                     /locus_tag="VXA80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0353051.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9612..10418
                     /locus_tag="VXA80_000508"
     CDS             9612..10418
                     /locus_tag="VXA80_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0353052.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10428..11933
                     /gene="adcA"
                     /locus_tag="VXA80_000509"
     CDS             10428..11933
                     /gene="adcA"
                     /locus_tag="VXA80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV0353053.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13036..13293)
                     /locus_tag="VXA80_000510"
                     /pseudo
     CDS             complement(13036..13293)
                     /locus_tag="VXA80_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            13469..14872
                     /locus_tag="VXA80_000511"
     CDS             13469..14872
                     /locus_tag="VXA80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV0353054.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNIVVQLLSQGKINEDTGLK
                     DIMTNIADTQYYYPQLS"
     gene            14901..16193
                     /locus_tag="VXA80_000512"
     CDS             14901..16193
                     /locus_tag="VXA80_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353055.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPEQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKSVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            16280..17209
                     /locus_tag="VXA80_000513"
     CDS             16280..17209
                     /locus_tag="VXA80_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0353056.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIFCINILFRERKRLKGGK"
     gene            17212..18051
                     /locus_tag="VXA80_000514"
     CDS             17212..18051
                     /locus_tag="VXA80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0353057.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            18048..19367
                     /locus_tag="VXA80_000515"
     CDS             18048..19367
                     /locus_tag="VXA80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0353058.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNENTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            19373..21244
                     /locus_tag="VXA80_000516"
     CDS             19373..21244
                     /locus_tag="VXA80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV0353059.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDMRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDSQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            21266..23482
                     /locus_tag="VXA80_000517"
     CDS             21266..23482
                     /locus_tag="VXA80_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0353060.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLLQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            23493..26509
                     /locus_tag="VXA80_000518"
                     /pseudo
     CDS             23493..26509
                     /locus_tag="VXA80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
     gene            26524..27252
                     /locus_tag="VXA80_000519"
     CDS             26524..27252
                     /locus_tag="VXA80_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV0353061.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            27265..29031
                     /locus_tag="VXA80_000520"
     CDS             27265..29031
                     /locus_tag="VXA80_000520"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV0353062.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            29177..30328
                     /locus_tag="VXA80_000521"
     CDS             29177..30328
                     /locus_tag="VXA80_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV0353063.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMISKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            30473..31357
                     /locus_tag="VXA80_000522"
     CDS             30473..31357
                     /locus_tag="VXA80_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0353064.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         9977 a         5110 c         6631 g         9698 t
ORIGIN      
        1 gcacctgcta ccggtgaacc caatagcgat tcccaagcct gactatcgtg aggtagcagg
       61 gagaggaagg gatagcgaaa tcgtggctct acgaacagga acgtgatagt aaggcgtata
      121 tagcggataa ggaggcttca aactctaaag tccaaaaagg tagtcgtaac ctatatgtgt
      181 aaatcatgag agtaattgaa ttcggactaa ggtttgtgtg aaaaagataa atctttctag
      241 agtctaaaga ctctgcgtca gatttcctat tttcactgta accttttaac gtcctcatat
      301 cttgtataaa cgaggaaaga tgtacgactt atcccgtgag gtttcatgag cgctgaaagc
      361 gtagtaacaa cgaatcatga gaagtcagcc gagcccatag tagtgaggaa acttctgtag
      421 tggaagtgga gcgaaggggt gaatactcaa acagtctggg gagagactgt ttgaggtctg
      481 tcgctagaaa gagaaaacga cagatcgaag taatcctact tcacttgtgt ctgtaaaatg
      541 agtggtctga tagaactgga ctttgacacg taatgactag atgaaggttc accaatataa
      601 gatgtccctc aggcaccaaa acaagaaagg aatacgcaca tgtcaaaact gctagataag
      661 atattatcac gcgaaaatat gctggaagcc tacaatcaag taaaatccaa taaaggctca
      721 gctgggattg atggaatgac tatcgaagag atggataatt atctcagaca aaactggcgc
      781 ttgactaagg aactgataaa acagagaaaa tataagcctc aaccagttct taaagttgag
      841 atacctaaac cagacggagg catccgtcaa ctaggaattt caacagttat ggatagaatg
      901 attcaacagg ccattgtcca agtcatgagc cccatttgtg aaccccattt ctcagatacg
      961 agttatggtt tcagaccaaa taggtcatgt gaaaaagcca tcatgaagct cttagaatac
     1021 ttaaatgacg gctatgagtg gatagtggat atagacctag agaaattttt cgatacagtt
     1081 cctcaagata gattgatgtc cttagtacat aacattatcg aagacggaga tacggaatcc
     1141 ttgattcgta agtatcttca ttcaggtgtt atcattaatg gtcaacgtta taaaacacta
     1201 gttggtacac cacagggagg aaatttatct cctctcttat ccaatatcat gcttaatgaa
     1261 ttggacaagg aattagaaaa gaggggactt cgatttgtgc gctacgcaga tgattgtgtg
     1321 atacggtcgg aagcgaggca gccgctaagc gtgtgatgta ttcagtcagt cgttttattg
     1381 agaaacggct aggtttgaaa gtaaacatga ccaagactaa gattaccaga ccaagagagt
     1441 tgaaatatct aggttttggg ttctggaaat catcagatgg ttggaaaagt cgtccacatc
     1501 aagatagtgt tcggagattt aagcttaaat tgaagaaact aacacagagg aaatggagta
     1561 tagacctaac aagacgtatt gagcaactga atttgtctat tcgaggatgg ataaactatt
     1621 tctcattggg aaatatgaaa agtatagtcg ccagcataga tgagcgcttg cgtactcgcc
     1681 tacgagtgat tatctggaag caatggaaga agaaatcgag acgattatgg ggattgctta
     1741 agttaggagt tcctaaatgg atagcagata aggtatctgg ctggggcgac cattatcaat
     1801 tagtagctca gaagtcggta cttaaacgtg ctatatcaaa accagtcctg gaaaaacgtg
     1861 gactggtttc gtgtttggat tattaccttg aacgacatgc gttaaaagtt agttgaaccg
     1921 ccgtatgccg aacggcacgt acggtggtgt gagaggggct agagattatc ccctactcga
     1981 ttaactcccc tgaaatttat tttaattatg caaatttcac gtatttttga tgctgagacg
     2041 acgatcctgg gaacttttca gatatttttt tgactatcta aatctatcat tagaaaagct
     2101 tagagcgcca aaggatttga gcgtttttct gatttttaag actttttcca gtctcttttt
     2161 cgattgaaga tgtaattatt ctactaacta actaacttct tagtactagc caacaacgat
     2221 aatcataatt cctcctaaaa ttaggaataa taaaggcaat agtttttgtt ttttcatgta
     2281 aaaaacctca cttttgtttt ctgctatttt atgctaaaat attaaaaatc aaatttaatt
     2341 ccaaagtttg taactaaagg gggagcgcta catgtctaat tcatttgtca agttgttagt
     2401 ctctcaatta tttgcaaatt tagcagatat tttctttaga gtaacaatca ttgctaacat
     2461 atacattatt tcaaaatcag taattgccac atcactagtt cctatcttaa taggaatatc
     2521 ctcttttgtt gcgagtcttt tagttccgtt ggttactaaa aggttagcgc taaatagggt
     2581 tttatcttta tctcaatttg gaaagactat attattggcg atactggtag gaatgtttac
     2641 cgtaatgcaa tccgtagcgc ctttggtgac ctatctattt gttgttgcaa tttccatact
     2701 agatggtttt gcagcacccg tttcctatgc tattgtgcca cgctatgcga ccgatttggg
     2761 taaggctaat tcatccttat caatgactgg tgaagctgtt caattgatag gttggggatt
     2821 aggtggactc ttgtttgcaa caattggtct gttacctacc acgtttatca ttttagtctt
     2881 gtatatcatt tctagctttc tgatgttatt tcttcctaac gctgaagtgg aggtgttaga
     2941 gtcagaaact aatcttgaaa ttttgctcaa aggttggaag ttagttgcta gaaatcctag
     3001 attaagactt ttgtatcagc aaatttattg gaaatttttt caaatacgat ttgggtttct
     3061 tccattatac ttgtttttgt aacggagtta ttaaataaaa cggaaagtta ctggggatat
     3121 tctaatacag tatactctat tggtattata attagtggct taattgcttt taggctatct
     3181 gaaaagttcc ttgctgctaa atgggaaagt attctttttc ctcttgtagc tatggcaata
     3241 gtgacactga ctattctata ttttccaaac gcacagatgt ttttattatt ttcagcttta
     3301 gttggtatgt tatcgcaact aaaagaagtt cctgaaagtg tatttcttca ggaaacagta
     3361 gaggaaaatc atttagttaa tgtctattcc gttcttgaag tgatttctac attagcattt
     3421 tcagtatttg ttttgctaat gagctatatt actgagagtt ttggtattag catcagtttt
     3481 tggctatcag ccatttgtct gatgatagaa gctattttaa tttatatcag acgagattat
     3541 tttaaatgag agagtggtta aaaagtcttt aaaaatcaga aaagcgcata gtatcaggtg
     3601 ttgaaaaaac ttgatattgt gtgttttatt atgggaagat ttacttcatt gtctcctgaa
     3661 atggagtttt gaagcaggat cactctattt gaaaatatac atagaaaaac cgcataatcc
     3721 ttaaaataag ctgaaatcta ttggaaacat acggtaaaca attgaaaaga tactatataa
     3781 agtggtacaa tggtagaaaa ataagctagt aagaatcact cttattttag cgaaaaatta
     3841 cagaaatgaa tggtttttct tgatgaaaca gagaaaagaa ttgtacctct ttcttggtcg
     3901 gacagccttg tattttctta tctttctagg gctgctttac ttctttagct atcttggtca
     3961 gggtcaagga agctttatct ataatgaatt ttaacttgaa ggagaatccc tattgtgtca
     4021 aataaaccaa tagcagatat gattgaaacc attgagcatt ttgctcagac acagcctagc
     4081 tatcctgtct ataatgtttt ggggcaggaa cacacttatg gcgatttaaa ggctgattcg
     4141 gatagtttgg ctgcagtcat tgaccaacta ggcttgcctg aaaagtctcc tgtggttgtt
     4201 tttggtggcc aagaatatga aatgttggca acctttgtag cgctgactaa gtcaggtcat
     4261 gcctacattc caattgatag ccattcggcc ttggagcgag tttcagctat tttagaagta
     4321 gcagagccaa gcttgattat tgccatttca gcctttccct tggagcaggt ttctacacca
     4381 atgataaatc tagctcaggt tcaagaagcc tttgcccaag ggaataacta tgaaatcacg
     4441 catccagtca agggagatga taattactac attatcttta cttctggtac gactggtaag
     4501 cctaagggag tgcagatttc acatgataat ctcctcagct ttacaaactg gatgattacg
     4561 gataaggaat ttgcgacacc gagtcgtccg caaatgctgg cacagccacc ttattctttt
     4621 gacttgtctg tcatgtattg ggcaccgacc ttggcacttg gtggtacgct tttcactctt
     4681 ccttcagtca tcactcagga ctttaagcaa ctctttgcgg ctatcttttc attgccaatc
     4741 gctatctgga catcaacacc atcctttgca gatatggcca tgttgtctga atacttcaac
     4801 agtgagaaaa tgcctggaat cacgcatttc tactttgatg gtgaagaatt gacggtcaaa
     4861 acagctcaaa aactgcgcga gcgtttccca aatgcccgta tcatcaatgc ttacggccca
     4921 acagaagcga cagtagctct gtcagcagtt gccgtgacag acgagatgtt agcgactctc
     4981 aaacgcctac caatcggcta taccaaggct gattctccaa cctttatcat tgacgaggaa
     5041 ggaaataaac tgccaaatgg cgaacaggga gaaatcattg tttctgggcc agctgtttca
     5101 aaaggttata tgaacaatcc tgaaaaaaca gcagaagcct tctttgagtt tgaaggtctg
     5161 ccagcctatc acacaggcga tgtgggaacc atgacagatg agggcttgct tctctacggc
     5221 ggacgcatgg acttccagat taagttcaac ggttaccgca ttgagttaga agatgtctct
     5281 caaaacctca acaagtctcg ctttatcgaa tctgctgtcg cagtaccgcg ctataacaag
     5341 gaccacaagg tacaaaatct attggcttat gtcatcttaa aagacagtgt tcgtgagcag
     5401 tttgagcgag atatcgatat taccaaggcc atcaaggaag acctgacaga catcatgatg
     5461 tcctacatga tgccatctaa attcctttac cgagacagtt tgccactgac tccaaatgga
     5521 aagattgaca tcaaaggatt gattaacgag gtgaataaga gatgatggag ttttttcaac
     5581 agcttcctca tttagagcca tatggcaatc ctcagtattt tgtttatgtg attgctgcaa
     5641 ccttgcccat ctttataggc ctctttttca agaaacgctt tgcctggtat gaagtgttgg
     5701 taagtctctt ctttattgtc accatgttgg tgggtggaaa gaccaatcaa ctagctgcct
     5761 tgggtattta cctttgctgg gaaatattgc tcctgctttt ctacaagcat tatcgaaaaa
     5821 gcaaggatgg caagtgggtc ttctacttag ttagttttct gtccctactt ccgattatct
     5881 ttgtcaaggt gcaaccagct atcaatggaa cgcagtcttt gcttgggttc ttgggaattt
     5941 cttacctgac ctttcgttcg gttgggatta tcatcgagct gagagatgga gtgattaagg
     6001 attttaccct ctgggaattc ctccgtttcc ttctcttcat gccaactttc tcgagtggtc
     6061 caatcgatcg ctttaagcga tttaatgaaa attatcaggc tattcctgag cgagatgagt
     6121 tgatggatat gctggatgaa tctgtccgct atatcatgtg gggctttttg tataagttta
     6181 tcctagctca tgttttagga gagaccttac tacctcctct gaagaattta gccttgcagt
     6241 caggtggctt ctttaatctc tatgccttgg cagttatgta tacttttggt ctggagctct
     6301 tctttgactt tgcaggttat tctatgtttg ctttggccat ctcaaacttg atgggaatcc
     6361 gtagccctat caactttaac aagccctttt tatcaaggga tttaaaggag ttttggaatc
     6421 gctggcatat gagtctgtcc ttctggttcc gtgactttgt ctttatgcga atggtgatgg
     6481 tgttaaccag aaagaaagtc tttaaaaatc gtaatgtaac ctcaagcatg gcctacattg
     6541 taaatatgct gattatggga ttttggcatg gtgtgacctg gtactatatc gcctatggac
     6601 tctttcatgg actaggcttg gtcatcaatg atgcctgggt tcgcaagaaa aaaacgctca
     6661 ataaggaacg gaaaaaagca gggaaggctg ccctacctga gaatcgctgg attcagttgc
     6721 ttggcatggt tgtcactttc catgttgtca tgttgtcatt cttaatcttt tctggattct
     6781 tgaataatct atggtttaaa aaataaaagg aaataaaata tggatatcaa atcagaagtt
     6841 attgaaatta ttgatgagtt gtttatggaa gatgtttctg acatgatgga tgaagatctt
     6901 tttgatgcag gtgtcttgga tagtatggga acggttgagt tgattgtgga gattgagaac
     6961 cgttttgaca ttcgtgtccc tgtaacagag tttggtcgtg acgactggaa tacagctaat
     7021 aaaatcatag ctggtattgt ggagctacaa aatgcttaaa cgcttatgga tgatcttcgg
     7081 accggtcttg atcgctggtt tgttggtttt tctgctcatt ttcttttatc ctactgagat
     7141 gcatcataat ctaggagctg aaaagcgttc agcagtggct actactatcg atagttttaa
     7201 ggagcgaagt caaaaagtca gagcactatc tgatccaaat gtgcgttttg ttcccttctt
     7261 tggctctagt gaatggcttc gttttgacgg tgctcatcct gcggtattag ctgagaaata
     7321 caatcgttcc taccgtcctt atcttttagg acagggggga gctgcatcgc ttaaccaata
     7381 ttttggaatg caacagatgt taccacagct ggagaataaa caagttgtgt atgttatctc
     7441 acctcagtgg ttcagtaaaa atggctatga tccagcagcc ttccagcagt attttaatgg
     7501 agaccagttg actagttttc tgaaacatca atctggggat caggctagtc aatatgcagc
     7561 gactcgctta ctgcaacagt tcccaaacgt agctatgaag gacctggttc agaagttggc
     7621 aagtaaagga gaattgtcga cagcagacaa tgaaatgatt gaattattgg ctcgttttaa
     7681 tgaacgccaa gcctcctttt ttggtcagtt ttcggttaga ggctatgtta actacgataa
     7741 gcatgtagct aagtatttaa aaatcttgcc agaccagttt tcttatcaag caatagaaga
     7801 tgttgtcaaa gcagatgctg aaaaaaatac ttccaataat gagatgggaa tggaaaatta
     7861 tttctataat gagcagatca agaaggattt gaagaaatta aaggattctc agaaaagctt
     7921 tacctatctc aagtcgccag agtataatga cttgcagttg gttttaacac agttttctaa
     7981 atctaaggta aacccgattt ttatcattcc acctgttaat aaaaaatgga tggactatgc
     8041 tggtctacga gaggatatgt accaacaaac ggtgcagaag attcgctacc agttagaaag
     8101 tcaaggtttt accaatatag cagatttttc taaggacggc ggggagcctt tctttatgaa
     8161 agacaccatt caccttggtt ggttgggttg gttggctttt gacaaggcag ttgatccttt
     8221 cctatccaat cccacaccag ctccgactta ccatctgaat gagcgctttt tcagcaaaga
     8281 ttgggcgact tatgatggag atgtcaaaga atttcaatag ataatataat atagaagagt
     8341 tcaagaatga aactagtttt cacgtttttt cttgactctt tttttgcttg tgatataatt
     8401 aactggtaaa caaaatgtca aagaatagta tttttctctt aaaaaagaga agtctaaaag
     8461 gaaaggagtc agatatgaga cagctagcaa aggatatcaa tgcttttttg aatgaggtga
     8521 ttttgcaggc ggaaaatcag catgaaatcc taataggtca ttgcactagc gaggtggccc
     8581 tgaccaatac tcaggagcat atccttatgc tcttgtcaga ggaatcttta acaaattcag
     8641 aattggcccg tcgtctcaat gtcagtcagg cggcagttac caaggccatt aagtctttgg
     8701 tcaaggaagg gatgttggaa acatctaaag attctaaaga tgcgcgtgtg attttttatc
     8761 agttgactga cttggctcgt ccaatcgctg aggagcacca ccatcaccat gagcatacac
     8821 ttttaaccta tgaacaagtg gcgactcagt ttactccaaa tgaacaaaaa gtgattcagc
     8881 ggtttttgac tgctttagta ggagaaatca aataatgaga tatattacgg tagaggattt
     8941 gtccttctat tatgataagg agcctgttct tgaacatatc aattatagtg ttgatagtgg
     9001 ggaatttgtt accttgactg gggaaaatgg agcggctaag acgacgctca tcaaggccag
     9061 tcttggaatt ctgcaaccac gcattggaaa ggtggctatt tcaaagacaa atacgcaagg
     9121 taagaaattg agaatagcct atcttcctca acaaattgcc agttttaatg ctggttttcc
     9181 aagtacggtc tatgaatttg tcaagtcggg tcgctatccg agaaaaggct ggttccgtcg
     9241 tttgaatact catgatgagg agcatatcaa ggctagtctg gactcagttg gcatgtggga
     9301 acatcgagac aaacgcttgg ggtctctatc tgggggacaa aagcagcgag cggtaattgc
     9361 gcgtatgttt gcttctgacc ctgatgtgtt tatcctagac gagccgacaa cggggatgga
     9421 tgcaggaagt aaaaatgaat tttacgaact catgcatcac agcgcccatc atcatggcaa
     9481 ggctgttttg atgattaccc atgaccctga agaagttaag gattatgcgg atcgcaatat
     9541 tcatctagtc cgtaaccaag actcgccatg gcgttgtttc aatgttcatg agaatggcca
     9601 ggaggtgggc catgcttagt ttattatctt acgactttat acaacgcgcc tttttggcgg
     9661 ttattgctat gagtcttttc tcaccggtat tgggaacctt cctcatcttg cgtcgtcaga
     9721 gtttgatgag tgataccctt agccacgtct cactttcagg tgtagccttt ggtctggttt
     9781 tggggatttc tccaactgtt tctactattg ccattgtctt gattgcggcg gtctttctgg
     9841 agtatctccg tacggtttac aagagcttta tggaaatcgg gacagctatc ctcatgtcaa
     9901 caggtctggc tgtttctctg attgtcatga gcaagggtaa aagctcgagt tcaatgagtt
     9961 tggaccaata tctctttggt tcgatcgtga ctatcagtga agaacaggtc atttccctct
    10021 ttgtcattgc ggcggttgtt ttgattttga cctttctctt tcttcgtcct atgtatatct
    10081 taacttttga cgaagatacg gcctttgtgg atggcttgcc agttcgtacc atgtccattc
    10141 tttttaacat ggtgacaggg gtggctattg cccttatgat tcctgcagca ggagctcttc
    10201 tggtatcgac cattatggtc ttgccagcta gtattgccct gcgtctgggg aaaaacttta
    10261 aatcggttat gctgcttgcc agtgcgattg gctttttggg aatggtagca ggactttaca
    10321 tttcctacta tgcagaaaca cctgcaagtg caagtattac cattattttt gtaactgtct
    10381 ttatactaat cagtttagta agacgtttta tcaaatagga gacgaacgtg aaaaaaatta
    10441 gcttattact agccagtcta tgtgccttgt ttttagtggc ttgttccaat caaaaacagg
    10501 cagatggtaa actcaatatc gtgacaacct tttaccctgt ctatgaattt accaagcaag
    10561 tcgcaggaga tacggctaat gtagaactcc taatcggtgc tgggacagaa cctcatgaat
    10621 acgaaccatc tgccaaggca gttgccaaaa tccaagatgc agataccttc gtttatgaaa
    10681 atgaaaacat ggaaacatgg gtacctaaat tgctagatac cttggataag aaaaaagtga
    10741 aaaccatcaa ggcgacaggc gatatgttgc tcttgccagg tggcgaggaa gaagagggag
    10801 accatgacca tggagaagaa ggtcatcacc atgagtttga cccccatgtt tggttatcac
    10861 cagttcgtgc cattaaacta gtagagcaca tccgcgacag cttgtcagca gattatcctg
    10921 ataaaaaaga gacctttgag aagaatgcag ctgcctatat cgaaaaattg caagccttgg
    10981 ataaggctta cgcagaaggc ttgtctcaag caaaacaaaa gagctttgtg actcaacacg
    11041 cagcctttaa ctatcttgcc ttggactatg gactcaaaca agtcgcaatc tcaggccttt
    11101 ctccagatgc agagccatca gctgctcgct tggcagaatt gacagagtat gtcaagaaaa
    11161 ataaaatcgc ctatatctat tttgaagaaa atgcctcaca agcccttgct aacacacttt
    11221 caaaagaagc aggtgtcaaa actgatgtcc tcaatccttt agaaagtctg acagaagagg
    11281 acaccaaggc tggagaaaac tacatttccg tgatggagaa aaacctcaag gctttgaaac
    11341 aaacaacaga ccaagaaggc ccagcaatcg aacctgaaaa ggcagaggat accaagacag
    11401 tccaaaatgg ttacttcgag gatgcagctg tcaaggaccg caccttgagt gactatgcag
    11461 gtaactggca atcagtttat cctttccttg aagacggcac gtttgaccaa gtctttgact
    11521 acaaggctaa gttgactggt aagatgaccc aggctgagta caaggcttac tatacaaaag
    11581 gctatcagac agatgtgact aagattaaca ttactgataa tactatggaa tttgttcaag
    11641 gtggacaaag caagaaatac acttacaagt atgtcggtaa gaaaattttg acttacaaga
    11701 aaggcaatcg tggcgtgcgt ttcctctttg aagccacaga tgctgacgct ggacaattca
    11761 agtatgttca gtttagtgac cacaatatcg ccccagttaa ggcagaacat ttccatatct
    11821 tttttggagg tacaagccaa gaaactctat ttgaagaaat ggataattgg ccaacctact
    11881 acccagataa cctatctggc caagaaatcg ctcaagaaat gttggcgcat taatgagaaa
    11941 tcgactagtt cataagagct agtcggtttt tgggtactag atggctaact tcattataga
    12001 cttttcagaa ctaaggaact ttaaaaaaat aaataaaact cttgaaatat ctggacttat
    12061 atgctagaat gtagttagtg tataaggttc attagaacac caaagcccct aactagtgcc
    12121 atagttaagg gcttttttga cgtatcttag cgatttaacg ggtgtttata ggctagtcag
    12181 tcggtctact tcttaccatc tagccatttg cagacgaaat acaagataat tccagctata
    12241 acatctgcta taatagtaag agcaagctct ggtataaggc tcattagttt cacctcctct
    12301 cacgaaccgt aaggaaggta atcggtaacc gatgacaaaa gtattatacc acactaaatt
    12361 tagatcatca acctcactta atgcttgaac tattgtagat tatatgctaa aatgtagtta
    12421 attgataagg ttcattagaa caccaaagcc cctaactatg gccgtagtta agggcttttt
    12481 tgacgtatct tagcgattta acgggtgttt ataggctagt cactcggtct actccttact
    12541 atctagccat ctgcagacga aatacaagat aattccagct ataacatccg ctataatagt
    12601 aagagcgagc tctatgataa ggctcattag tttcacctcc tctcacgaac ccataggaac
    12661 gtaatcggta accgatgaca aaaatagtat accacaatac atttagatca tcaaggtcac
    12721 ttaattcttg aaatataggg tctaggcgac aaagtcttta aaatcagaaa aacgcataat
    12781 atcaggtgtg caaaaacttg atactatgcg ttttattgtg ggaaggttta ctccattttc
    12841 tcctgaaatt gagtttttgt ccagcctctg tttttagaat tgctaagaaa ataatgtcat
    12901 gtggtgaata tttgtaaatc agtcagcaga cagaacgaat actcttcgaa aatctcttca
    12961 catcatgtca gcttcgtctt gccgtgtcca tgggggactt gacttcatca gttttcaaat
    13021 ctgtactttg agcaagctga gactagcgat tagtcttttt tgcctagtac tttctccttc
    13081 ccgtatttga tgagaaagtt caattgtgtt catagaggac agggtcacgt atccgtgctt
    13141 tcttttgata agaccttgat tttctaagaa aattaaatca cgacgtaagg tacttgtgct
    13201 ggagaaagtg atttctgcca gctcttttac ggcaattctt tttttctttt tgatgatttc
    13261 aatcaattca agtacacgtt catcttttat cataagctcc tcctaattta tcatttcaac
    13321 tatattatag cacaaattgg aggaatgtga attattttta tgaatattgg gttaacattt
    13381 gaacattatt caagtaagcg ttcacatatt gaaaaaataa aacgtgggga ttataataaa
    13441 gttaatcaag gacgaagaga gaagaaaaat ggaagcggtt ttagcaatag atttaggtgc
    13501 gacttctgga agagcaatcg ttggttacct ttctgaaaat aaactagtaa tggaagaaat
    13561 aaatcgcttt tctaatctac ctattagagt aaaagggcat ttatcttggg atgttgactt
    13621 tctactagct aaaattcttg aaagtatccg cttggctaat actagttaca agattttatc
    13681 tatcggtatt gacacatggg gagttgattt tggactgatt gataatgaag gtaagctgtt
    13741 atcacaacct gttcattatc gtgatgaaag aacaaaggga gtgttaaagg aaatatctga
    13801 aatgactgaa ttagaaaaac tgtattcaga gacaggaaat cagattatgg agataaatac
    13861 cttgtttcaa ctctttaagg cacgtcaaga atctcctgac tctttctata agaccaataa
    13921 gattctttta atgccagatt tgtttaatta tctcttgaca ggtaagtttg ctacagaaaa
    13981 aagcattgct tcaacaactc aattatttga tcctaggagt caaaactgga atcagaatat
    14041 cttaaaacta tttgaattgg attcatcttt acttcctgag attgtttcag agggaaatgt
    14101 tcttggaagg ataaaaaagg agtatggttt aggcgatatt cctgttgtga atgtttgtag
    14161 tcatgataca gcaagtgcga ttgtctcagt acctaagaca gaaggtagtt tatttatttc
    14221 atcaggtact tggtctttgg ttggagtgga acttacttca ccgattctta ctaccgaatc
    14281 cttcagttat ggatttacaa atgaagtcgg taaagatgga gagattacat ttctgaagaa
    14341 ttgtacaggg ttgtggatca tagaggaact aagacgttca tttgaacgaa gagggaaagc
    14401 ctattctttt gatgatatta ggacaatggt ggagaaagaa aaagaaaatc ttcctctgat
    14461 tgatactgaa tcaacagaat ttgcaacaga atctgatatg cacaagactt tgacagaata
    14521 tctagcttat catcatgaaa ctagagagtg gacagatgga caactattta agattgttta
    14581 tgaaagccta gctgaaacgt ataggaaagc gatagagtta ttagaagaac taactcataa
    14641 ggtttataag aggatatatg tgattggagg aggtgctaga gccagttatt ttaaccaaat
    14701 gattgctgat agaactggta aagaagttct tacaggttca actgaggcta cagctgtggg
    14761 gaatattgtt gtgcagctat tgagtcaagg taaaataaac gaggatacag ggttaaagga
    14821 tattatgaca aatattgctg atacacaata ttattatcct cagctatcat aaacaaaaat
    14881 aaaaaaacgg aggttttgtc atgaacaaaa aaagtctttt gaaatgcgct gttatcgggt
    14941 tagtagctac ctttggttta gcagcttgtg gaacatctaa ggatgcaagc ggtggaagtt
    15001 cttctggtaa agaagtatta gaattttatc atgggtatca tcatagtgaa gatgaatggc
    15061 ctgtagcgaa gactatgcga gatttatatg ataaatttgc agaagaacat aaagatagtg
    15121 gtgtagaatt taaaccaact cctgtaaatg gtgatttgaa ggatataatg aataacaagg
    15181 ttgctagtgg tgaatttcct gatgtgattg acctagcagg taatgcagta tcattagctg
    15241 cgattgaaca aaaacttgtt ttagatttga aaccatatat cgattcaaat aagcttgaaa
    15301 aaaatgttgg tttgaactat aagcaaaacc aaaaagatgg aaaaatttat acagttcacg
    15361 aacagttatt tacaatgggt ttgtggtaca acaaggacat atttgcaaaa gcgggtgcta
    15421 agacacctga gcagtggaat acttgggatg atttcactca ggcaatggca agcattagaa
    15481 aacaagatgg agtgtatgca tttggtgctg gagaaccatc tattcgcttg tttaatacag
    15541 tattaggaac cacagaaaat ggacgtaaat tattagataa accattaaca aaagaaggaa
    15601 ttgaatctaa agaatttgca gatgctttga aaatggttat gaaagaaatt caagctaatg
    15661 gttctaagaa tgctggtggc gatgccaatg cttattccaa agatttccaa gaaggtaaat
    15721 ctgcagtatt ctttaatggt gtgtgggctt ctggtgagat gtctaagaac ccaagtcttg
    15781 cacctggaat ctatcctgca ggtgtagcaa ttagttcttc tggaggtggt atcactatct
    15841 caagtaagat gtctgaagct aaacaaaaac tagctcttga gtttttgaaa tatatgacta
    15901 gcgatgatgt acaaaaagta atttttgaaa aagtaggagc aaatccttct aatgaaaatg
    15961 taaatgtaaa agaactttca gaaaagagtt cagaagctac tactaaaatt ttaggacaag
    16021 caatcactca agttaagaat gcaaaatcag ttgttccaac tgtaagtgat gtttggggtg
    16081 gcgatgtaca cacagctatt atcaatgcat tgacagaaag tgctgctgaa aatgtagatg
    16141 ttgatcaaaa agtaaaatct actcaagatg tgttgaaatc attgattggt taattgtttt
    16201 agggagtgaa aacgaagctc agttggcact gccaactgag cttgatttta cttatatagt
    16261 tgaaaaaagg agtttgttta tggcacaaaa aattatgagc ctacaaaata ggaagaatca
    16321 aaaaagacga tttatctttt tatttctttt gccaacgttg atttgtttct ttctatttta
    16381 tttttattct gtagtaacaa tctttttaac ttcatttgct aaatgggatt atacaaattt
    16441 aaacacccct gagtttcttg gatttgataa attatttgaa aattataggt atgtttttaa
    16501 agaataccca ttttttacag aagctctaat taattctgtt cgttgggctg taattggtgt
    16561 tattattcaa gttcctttag ctgtatctgt tgcaattaca ttatctaaga aattaaaagg
    16621 ctggaagata tctaggaatc tttatattgt accaagtatc atttctagtg ctgctatggg
    16681 cttgattttt cttcaaatct ataatccaaa ctatggtgtt gttaaccaaa ttattcatct
    16741 atttaatcca tcgtttaaag attcagtact gttgactcca ggattaaata tagtagctat
    16801 gactggcgct tatatcttct ttgcaggagc atcaaccatt atgattcttg ggcaaatttt
    16861 tgctattcca gaagaagttc aagaagctgc tattttagac aatattactg gttggaggaa
    16921 agagtggtat attacgattc cgatgattaa ggggacaatt aaaactgttt caattatggc
    16981 agcaacttca gggtttttgc tctataacga agtattcttt ttgacaaatg gtgctgcagg
    17041 aacaaaaagt atcagttttg ttattcgaga attagcagtg gctagctcac gaactcagta
    17101 tgctcgtgca aatacaattg gagttataca aattttaggt ggaatgttga ttatcttttg
    17161 tattaatatt ttattcagag aaagaaaaag actgaaaggt gggaaatgat tatgaataca
    17221 catataaatg gtattagtaa aaaaggcaaa gttcttatat atggttatat gctccttacc
    17281 attttaattt ctattttccc tattgcgtgg atttttttat catcattaaa agcagatcct
    17341 atgaaaaatc caggtattag tttaccgact gactttactc ttgaaggcta tataaatgtt
    17401 tttacaaaac ttcatgtttt cacttacttt tggaatagct ttaaagtagt gtctatatca
    17461 gttattatta gcattgttat gatctccatg tcttcatatg ttattgctag gatggaattt
    17521 agagggaaga agttggtgac ttctatgctg tattctactc tatttattcc agcaacagct
    17581 atgacttttc cagtttatag attagtaaat gagttgggga tttataatac cccagttgct
    17641 ctaattctgg tctactcttg tagtggaatt gctatgagtt tctttattat aaagaactat
    17701 tttgaaatta ttccaaaaga attggaggag gcagcagaga ttgatggtgc gacatatgct
    17761 caaacttttt ggaaagtgat gttaccaatt gctagaccag gaattttaac agcagcagta
    17821 cttgccttta ttaataactg gaatgaatat tattgggcat ctatgttggt gattgataaa
    17881 aatgaattaa cagtaccagc attactaggt caatttacta caagttttaa tactaattat
    17941 aatggtctat tttcagctat tgttgtaatc gtattaccac caatcattct attcgcattt
    18001 acaagtaaat acttcattga agctttagga ggaggagcag taaaaggatg aaacatacac
    18061 ttgaaactat caatagtaga actcagtggt ttagagaagc aagatttggg atgttcattc
    18121 attgggggtt atattctatt ccaggaaaag gggaatggat tcgtagtcat cagaaattat
    18181 ctattgaaga ttatgaacca tattttcgag cttttgatcc taaggaatat aaccctagag
    18241 aatgggctaa acaagcaaaa gctgcaggga tgaagtatat ggttttaacg gctaaacatc
    18301 atgatgggtt ttgtttgttt gattcaaaat ttacagatta taaagcaaca aatacacctg
    18361 caggtagaga tttagttaaa gaatttgtag atgctgttcg agctgaaggt ttaaaagtag
    18421 gcttatattt tagtttgatt gactggcacc atcctgattt ccctaaatat gctgatctaa
    18481 atcatccaat gagaggaaat gaagcctatc gagatgagaa aattaacttt gacagttatt
    18541 tggagtattt acacaatcaa gttaaagaaa ttgtaactgg ttatggtcag attgacatat
    18601 tatggtttga ttattcttat gaagacatgg tgggtgaaaa atggggagct tcaaaattaa
    18661 tagacatggt tcgtcattat cagccaaacg ttatcgtaga taatagatta gaaacatcag
    18721 gtgaaggatt cggaagtatc gttacagatg aaattacctc ttacgctgga gattttgtta
    18781 gtccagagca aattgttcct cacgaaggta ttcgaaactt taaaggtgaa cctgttcctt
    18841 gggaactttg tctaactatg aataacaatt gggcttataa tcctacagat tatctatata
    18901 agtctagtca aactttgata agaaagttag tggaatgtgt tagtaaaaac ggaaatatga
    18961 ttcttaatgt aggcccagat gctttgggaa gaattaatga ttcaagtaag aaaatattag
    19021 ataattttca tcgatggatg tctcgaaatg gtgaggcaat ttatggatgt agtggagatg
    19081 aaaatttacc aaaaccagat tggggatact atactcgtaa tgaaaatacg gtttatgctc
    19141 atgtatttga acaacctata ggtcctttag ctcttcttgg tattagtaaa gaaaacgtaa
    19201 aaagaatgag ctttcttcat gatggaagtg aggtgaaaat ttcagaaagc tggacgacca
    19261 atgcctacaa aggaatttgt tttgcacagt ttggagaagt tccacatttt acctatccat
    19321 taccagactt gattgatagc gtaatcaaaa ttgagttgag agagtagttt atatgaaaat
    19381 tgaaaataaa aatgtaagac gtaatttctt ttggggagat ggtcgatttt atactactga
    19441 tatcgtaaac aaacgggcag gagtaatgat aaaaaatgtt tcgaaagaag aatttaccat
    19501 tacattggag aatggtataa gactttcttc aaatcatttt tcagcaatcg ttagggaaga
    19561 gggagatatg cgaattcagg tttcttttgt ttgtccttcc attcgtttac gtttaatctt
    19621 tgaatcgagg gatgatgttc tttcgaagca attagtttta gaatcatcta ctgaggttat
    19681 taaatctgta gaggtagaga gttttgagtt tgaaacagaa gacaatattt tctatccgaa
    19741 aagacaagat tgtattaagg aaatggcgaa tttttccggt tattatgtag aattgggaca
    19801 acctgtttat gcgaattctt tattcttagg aatggaattt ccaatgtctg aaaacaaggt
    19861 agatggtaga cactatgtat caagatatta cttgggaact gttgtaaatc aagaaaagag
    19921 tttgtggtct tgtattattg ggggagcatg ttcttataaa aaagaagaga ttcaagaggc
    19981 attttttgaa tatgttgaag gaatagctca acctagttat ttccgtaaac agtataattc
    20041 ctggtatgac catatgaccg atattacaga ggaaggtatt ttaaaaagtt tttctgagat
    20101 tcgagatgga tttgaaaatc atggagttca tttagatgct tatgttgttg atgatggttg
    20161 gacaaactat caatcagttt gggaattcaa tcataaattc ccaaatggtt tgagaaatat
    20221 taaacatctt gtaaatggat ttggttcaag cctaggattg tggattggtc cccgaggtgg
    20281 ttataatggg acagaaatca ttatgagtga ttggttagaa gcacatccag agttaaatat
    20341 tggatctaaa aatttgattt caaatgatgt aaacgtggct gattttaact atctcaatca
    20401 aatgaagaaa aagatgttgg aatatcaaaa agaattcgat atcagctatt ggaaaattga
    20461 tggttggtta cttcaacctg acaaacctga taagagtgga ccgcacggta tgtataccat
    20521 gacagcggtt tatgagttct taattcaact gttgatagat ctaagaaagg agagaggagg
    20581 aaaagattgt tggttaaact tgacttctta tgtaaatcct agtccatggt ttttacagtg
    20641 ggtcaatagt ttatggattc aaatatctca agatgtaggc tttacagaga atgcaggtaa
    20701 tgatatcaat cgtatgatta cttaccgaga tagtcagtat caagaatttt tggaaaaacg
    20761 tgagatacag ttacctatgt ggtcgcttta taatcatgaa ccaatctatg ctgtcagtgc
    20821 aaatacctgg tacatggatc atcaaatgtt tgcatcaata ccagattttg aagcttatct
    20881 attatttatt tcaacaagag ggaatgcttt ttgggagttt cactattctt ttgatatgtt
    20941 tgatgaagaa cgctggaaag ccaatgctcg tgccgtcaaa tggatagagg agaattatca
    21001 aacactaaaa tatagtaaaa aaataggagg aagtcctgaa aaatttgaaa tttatggtta
    21061 taagtgtcac aatcagaaga cttctactga aattctctct ctgagaaatc ctgctcagat
    21121 taaacaaaaa ataaaaatag agaatctttc gatagagaat tttaccagag taattggaga
    21181 ttttactatt caagaagatg agatagaatt ggcaccgtac tctatcgtga tactgaagaa
    21241 gtaaagaaaa aattgtgagg tagagatgac gatttatatt aataaggacg agaccgtttt
    21301 tcatttggca atgaaagata gtagttatat ttttagaatt ttagaaaatg gggaacttca
    21361 acatctacat tttgggaaaa ggattcatgt caaggaaaat tataaccaat tgatggccta
    21421 tgaaaaaaga ggatttgaag tatctttttc tgaagaattt gaggatattc aacagtctat
    21481 gatacaaaat gaatattctt catatgggaa aggagatttt cggcatccag cctttcaagt
    21541 tcaaggaatg aatggtagta ggataacgac actaaaatat caaggttttg aacttgaaaa
    21601 agggaaaaat cgtcttaact ctctaccttc aacatttgat gatattggtc agtgtgcgga
    21661 aacattaacg attattttaa cagattccat attagattta actgttagac taaattacac
    21721 aatttttccg gaatacaatg tcttagttag aaatacggaa tttttaaata atagcaataa
    21781 taagttgact cttttgaaag caatgagctt acagctagat ctacctgata gtcaatatga
    21841 ctttattcaa ttttctggag catggctgag ggaacgtcag ttatatagaa cttcgcttag
    21901 accaggtatt caagcaatag atagcttgag atactcatca agtcctcagc aaaatccttt
    21961 ctttatgcta tcaaggaggg aaactacaga gcatagtggt gaggtttatg gttttaactt
    22021 tatctattct ggaaattttc aaaatatgat tgaagttgac cattttgaca ccgctagagt
    22081 aacggtagga ataaatccag tagaatttcg ttttttatta aatcctgccg aaagttttgt
    22141 gacaccagaa gcaattgtga tctattctga tcaagggatg aatcagatga gccaacaact
    22201 atcagatttt tatcgacatc atttagttaa tcctaatttt tctcaagcta gtcgtcctat
    22261 aatactcaat agttgggaaa cattttattt tgacttgagt acagaaaaaa ttttagattt
    22321 agcaaaggct gctaaagatt tagggataga attatttgta ctggatgatg gttggtttgg
    22381 tcataggaaa gatgacaaaa gttctctggg tgattgggta acagatagaa gtcgccttcc
    22441 tgaaggtatt ggatttcttg cagatgaaat tcacaaaata ggtttacaat ttggtttgtg
    22501 gtttgagcct gaaatgattt ctattgatag tgatttgtac aagaatcatg ccgattggac
    22561 tatccatttg ttagacagag agaagtcagt aggaagaaat caatatgtgt tggatttgac
    22621 gagacaggaa gttgttgatt atctttttga ttctatttct aaaatcataa tcaaaacaaa
    22681 tctggattat atcaaatggg atatgaatcg tcatataaca gatatttata gtattgaact
    22741 tgattctgaa cagcagatgg aatttggtca tcgatatatc ttaggtcttt atcagttatt
    22801 agatcgttta ataactaagt tcccttcagt tctatttgaa tcttgttctt caggtggtgg
    22861 acgttttgat ttaggactta tgtattatgc accgcaagcg tggacgagtg atgatacgga
    22921 cccgatagaa agattgaaaa ttcagcatgg aacttcttat ggatattctc catcaatgat
    22981 gacagcccat gtttctattt ctccaaatga acaaagtgga agacaaacga gtttggacac
    23041 taggacaaat gtagcttatt ttagttcttt cggttatgaa ttagatgtta cgagattgtc
    23101 ggtagaagaa aaagaacaag tgagagaaca aattcagttt tataaaaaat atcgttcatt
    23161 gcttcaatat ggggatttct ataggataaa cagtcctttt agttgtgatt ctgctagttg
    23221 gcaagttgtt tcaaaagata aatgccaatc gattttattg tatgctcaat tgaatagtaa
    23281 gttgaatcca ggttatacaa gagtttattt tagtggttta gataaagata aatgctattc
    23341 cgtctctgga tttgatgagt tcttttatgg agatgaatta atgaatgctg gaataaaagt
    23401 aagtttaagt aatttagcac tttgtgttcc agaatatctt acaaaattat ttgttattga
    23461 agaagttgta tgtaaatatt gaggaaataa aaatggaaag taaaaaaata gcaaaacaga
    23521 ttttaatagc tacggctgtt ttaacaagtt ttttaggaag taatttggtt tatgcagatg
    23581 tagttcaaag taattctaat aacagagcta gtactgaaac tgctagagta acagggaata
    23641 acttagaaaa acttattatt aaagataaag aaattgataa agagatgaca tatctttcag
    23701 atatggattg gtcttctgca actcatggtg atatagataa aacaaaaaca gtacaaaaag
    23761 atgctccatt tacaacagga aataaaggag agcatactaa gatttcattg ctcacttctg
    23821 atgataaagt taaatatttt gataaaggaa taggaacagt cgcagattct ccatcagtaa
    23881 tcagctatga tatcagcggt caagggtttg aaaaatttga aacttatata ggaattgatc
    23941 aaagtgctaa tagttctaga tcagaccatg cagtagtgga tagaatagaa atagaaattg
    24001 atggaaaggt tgtctattct agtagtgtaa ctaatccaga aggattcaga tataataccc
    24061 aggcacaatt tattagtgtg acaattccac aaaacgctaa aaaaatatcc ttaaaatcat
    24121 tcgcaggaga acatacatgg ggtgatgagg ttgtatttgc tgatgctaaa cttattaaaa
    24181 ctgttagtac tcaaactata actccagatc ttcttaataa aggaattaat ggaggagtat
    24241 atctttctga tttagaatgg gttgatgcta ctcatggtga tgacgataaa tcaaaaacag
    24301 ttcagaaaga taaaccgttt acacctggta ataacgggtc taataataaa ataaaattac
    24361 taattgacgg taaggaagtc gaatttaata agggattggg aactgtagca agtaacccgt
    24421 ctagtataaa atatgatgtt tcgggtgcta atgttacaag atttatttcc tatgtcggaa
    24481 ttgataggag tgcaaatcat ttaaattcag attacgcaga tattcaaaaa tttgaagttg
    24541 ttgcggatgg taaagtcatt tactcatcag attcaaaata tccaaaagga attaagtatg
    24601 atacatcggc atttctggtt gatgttgaaa ttcccaaaga tacacagaca atagaactta
    24661 aatcatattc agggaaacat acatgggctg acgaattggt tttaggtgga gccttgttta
    24721 tggcaaatgg taaatttaag aatcctaatg attggtcaga agttgataaa cgtcgtgaaa
    24781 ttaataatga acatccgtta cttatgatgc ctttatatgc taacggagaa gagtttaacc
    24841 aagggaaata tactttttgg ggaggagata cattaacagg taagtgggag aatataccag
    24901 atgacttaaa accatatact gttatacagt tacatccaga tgatcttcct aaaagagatg
    24961 gagctgctag agatttttat gagcatatgt tagaagaagc agcgaaatat gtaaatccta
    25021 agactggaaa aatgaaccca ttccagttat tctaacggtt tacactgctg gtaatatgcc
    25081 ttattatact tcggcacatt ggttaagtac cagctggatt gataaaatgt atcaaaaata
    25141 tccaaatttg catggtattt tcagtactga gaattattgg atttgggcaa atgatattga
    25201 aaataaggcg gcagattact taaaagtatc agctaaaaat ggaggatatt ttatatgggc
    25261 tgaacaaaat aatggttctg caatagaaaa agcatttgga aaaaacggta agatagcatt
    25321 tcagaaatct gttgataagt actggaagaa ccttattttc atgttcaaaa atacacctgc
    25381 tgctgaggga aatgattcaa ctacagaaag ttatatgaag ggactttggc tttcaaatca
    25441 tacttatcaa tggggtggtt tgatggatac ttggaagtgg tatgagacag gtaaatggaa
    25501 actgtttgca agtggtaata tcggaaaaag tcaaggagat agacaatggt tgactgaacc
    25561 agagtctatg ctaggagaag aagctttagg ggtttacctt aatggaggtg tagtttataa
    25621 ttttgaacac ccagcttata cctatggtgt aaataataaa gaaagcttac tctttagcga
    25681 agtaataaaa gaatttttta gatatgtaat agcgcatcca gcaccatcca aagaaaaggt
    25741 acttgaggat acaaaagtat tcatacatgg agactattca aataaaggaa atggtaagtt
    25801 ctttgtaaac gtaaatactg atagagaaca aactccatta tacatgactg gtcgttataa
    25861 tgttattcca gctattccag gtgttttaaa aacagataaa ttgaaagagt ctgtttctgg
    25921 tagccgtatt cagattaagg aaatcacttc tccagaattt agctctactc aggctagaaa
    25981 agaatactta aataagctgt atcctatgaa ttatgaagga gatatctttg ctcagaaatt
    26041 agataataga tggtttgtct ataattataa ggtaaatgag aatgttaaac agactgggaa
    26101 attaaaattt aattctttag aaatggatgt tgaatttgaa cctcatacct atggtatttt
    26161 tgagagaata agtaatggat tgaaagtgaa tttaaataat tttagaacaa ataaagattc
    26221 actatggtct aatgctcaag atgcaaatca ggcaaagaaa ctaccacaat tgaccaagaa
    26281 aggtgccatt aagtggattg aagaacatta tatcaaagat actcagtttg gagagaaaag
    26341 ggttacaaag atagtattaa gaggtataga taaacttcct actattcatt cattatcagg
    26401 aacaaataat tcctatgatc agccatctct caattttgat cagaaaaatc atatggttac
    26461 tattaccatt aacagtaacg gaaatcttga attcgaactt catttttaga aaggagtgag
    26521 tttatgtctc aagatgaaaa attaattcgt gaacagattt gtgatgtttg tcataagatg
    26581 tggcaacttg gttgggttgc tgctaacgat gggaatgtat ctgttcgatt agatgaggat
    26641 accattcttg caacacctac tggtatcagc aaaagtttta ttacaccaga aaagctggtg
    26701 aagttaaatc ttaaaggaga gattttagaa gcagaaggtg attactgtcc ttctagtgaa
    26761 attaaaatgc acattcggtg ctacgaagaa cgtgaggatg ttcgttcagt tgttcacgcg
    26821 catccaccga ttgcaacagg atttgctctt gcacacattc ctttagatac ttattcacta
    26881 attgagagcg cgattgtggt aggggcaatt cctataacgc catttggagt tccgtctaca
    26941 atggaagtgc cagaagcaat tacaccttat ctgcccgatc atgatgtcat gctattagaa
    27001 aatcatggag ctctgactgt cggaagcgat gtaattacag catactaccg tatggaaact
    27061 ttagaattag tcgcaaagac aaccttccac ggaagaatgt tactttctac aaagggcatt
    27121 gaggagcaag aaattgctcg tccgacttta gaacgtctat tctcaatgcg agaaaattat
    27181 aaggttacag gtcgtcaccc aggctaccgt aaatataatg gcgatggtag tatgaaagaa
    27241 acagaaaaat aagaggaaaa tagtatgatt caacatccac gtattggtat tcgtccgact
    27301 attgatggtc gtcgtcaagg tgtacgcgaa tcacttgaag tacaaacaat gaacatggct
    27361 aaaagtgtgg cagatttgat ttcaagcaca ttgaaatatc cagatgggga acctgtggaa
    27421 tgtgtgattt ctccatctac cattggtcgt gttccagagg ctgcagcttc ccatgagttg
    27481 tttaaaaaat caaatgtttg cgcaacaatt acagttacac catgctggtg ttatggtagt
    27541 gaaactatgg atatgtctcc agatattcct catgctattt ggggatttaa tgggacagaa
    27601 cgcccaggag ctgtctatct tgcagctgta ctagcttcac atgctcaaaa agggattcca
    27661 gcctttggga tttatggtag agatgttcag gaagctaatg atacagatat tccagaagat
    27721 gtcaaagaaa aacttttacg ttatgcgcgt gcagctcttg caactggctt gatgagagac
    27781 actgcttacc tatcaatggg tagtgtttcg atggggattg gtggttctat tgtaaatccg
    27841 gatttcttcc aagaatactt aggaatgcga aatgaatcgg tagatatgac ggagttcaca
    27901 cgccgtatgg accgtggtat ttacgaccct gaagagtttg aacgtgctat ggtttgggta
    27961 aaagaacata tcaaagaagg agttgaccga aatcgtgagg atttgattct ttctaaagaa
    28021 gagaaagaaa aacaatggga atttgttatt aagatgttca tgattggacg tgacttaatg
    28081 gttggtaacc caagacttgc tgaacttggt tttgaggaag aagcagttgg tcaccatgct
    28141 ttagtagctg gtttccaagg tcaacgtcag tggacagacc attttccaaa tggagatttt
    28201 atggaaactt tcctcaatac tcagtttgac tggaatggta ttcgaaaacc atttgtattt
    28261 gcgacagaga atgattcact aaatggtgtg tctatgctct ttaattatct attaacaaat
    28321 actccacaaa tctttgctga tgtgcgtact tattggagtc cagaggctgt tgaacgtgta
    28381 acaggacata ctttagaggg tcgtgctgca gctggcttct tacatctaat caactctgga
    28441 tcttgtacat tggatggtac aggtcaagct actcgagatg gcaaacctgt tatgaaacca
    28501 ttctgggagt tggaagaaag tgaagtgcag gctatgcttg aaaatacaga cttcccacca
    28561 gcaaaccgcg aatacttccg tggaggagga ttctcaactc gtttcttgac gaagggggat
    28621 atgccagtaa caatggtacg tctcaatctt ctaaaagggg ttggtccagt gctacaaatt
    28681 gcagaaggtt acacacttga acttcctgaa gatgttcacc atactttaga taatcgtaca
    28741 gatccaggat ggccaactac ttggtttgct ccacgtttga caggaaaagg tgctttcaag
    28801 tctgtctatg acgtcatgaa taattgggga gctaatcacg gagccataac atatggccac
    28861 attggagcag acttgattac cttggcttct atgttgagaa ttcctgtcaa tatgcataat
    28921 gtacctgagg aagatatctt tagacctaaa aattggtcct tatttggaac agaagatcta
    28981 gaatcagcag actatcgtgc atgtcagttg ttgggaccac tacataaata aaacttgttt
    29041 atataggagg tgaacttacg tccctcctat ccttttaaaa agatttgtta aacaattcac
    29101 aaataattga aaacgaatac aaaaagtaat ataatgatgt taaatagata gcgcggaggc
    29161 gcaggaggaa aattatatgg ctatatttta tgttccggca gtcaacctta ttggaaaagg
    29221 tgttgtaaat gaagtgggtc cttatatcaa ggaacttggc tataaaaagg cacttttggt
    29281 tacagataag tacatcgaag gcagtgatat tttacctaag gttttaaaac cactggatac
    29341 agaaggaatc gaatatgtca tctttagcga tgtagagcca aaccctactt gtaagaatgt
    29401 cacagatggg gtagctgctt tgcaagaaca tggctgtgac tttatcatca gtcttggcgg
    29461 gggttctcca caggatgcag ctagttgtat ttctatcatg gctataaatg gtggaaaacc
    29521 acaggattat gaagggcttc ataagtctgc taaaaaaggc ttgccagttg tggctatcaa
    29581 tacaacggca ggaacttctg cagaaattac cattaactat gtgattactg atgaagaacg
    29641 caaggttaag atggtaatgg ttgacaagaa tagccttgcc ttgatttctg ttaatgaccc
    29701 tgaattgatg atttcaaaac ctcaagcctt gactgctgct actggtatgg atgctctgac
    29761 tcacgctgtt gaagctttgg taacacctgg tgcttatgat gtaaccaaga aactgtctat
    29821 tggtgctatt gagcttatca aggaatatct tcctcgtgct gtagcaaacg gacatgatat
    29881 tgaagcgcgt gaaggtatgg tcaatgccat cttccttggt ggtatgagct ttaataatgc
    29941 tggtcttggc tatgttcact caatggctca ccaactcggt gcagtatata atttgccaca
    30001 tggtgtttgc tgtgccatgt tgctaccagt tatagaacgt gaaaatgcta aacgtgtacc
    30061 agaagctttc cgcaatgttg ccaaagcctt gggacttcat gtagaaggta aatcagatca
    30121 agaatgtgcc gattatgcga ttgctgagat tgagaagctt tctgagacag taggtattcc
    30181 taagaaactg actgaacttg gtattgaaga aaaagatttc gactttgaat acctttctaa
    30241 gaatgccttg attgatgcct gcgcaccagg aaatccattt atgccaacct tagaagaaac
    30301 gattgccttt tataaagagt tattttagca agagattgga cgacaatagg acaattcacc
    30361 tctaattatt gacatttcag gaaaaatcgc agtaaaaacg gcacagcctt ctttaaatgt
    30421 gctataatac tagaaaaata cttgtggagg ttccattatg gcaatatttt tcatgatttt
    30481 tctgattgtt tgtgtgctcc tattggtgat agtcacactg agtacagttt atgtggttcg
    30541 tcagcagtcg gtggcgatta ttgaacgctt tgggaaatac caaaaggttg ctaatagcgg
    30601 tattcatatt cgcttgcctt ttgggattga ctcgattgca gcacggattc agttgcgctt
    30661 gttgcaaagt gatattgtgg ttgagactaa gaccaaggac aatgtgttcg ttatgatgaa
    30721 tgtagcgact cagtaccgtg tcaacgagca gagcgtgaca gatgcttact ataaactcat
    30781 acgtccagaa tctcagatta aatcttatat cgaagatgct cttcgctctt ctgttccaaa
    30841 attaaccttg gatgaattgt ttgagaaaaa agatgagatt gcccttgagg ttcaacacca
    30901 agtagcagaa gaaatgacca cttacggcta cattatcgtg aaaaccttga ttaccaaggt
    30961 cgaaccggat gcagaagtta agcaatccat gaatgaaatc aatgcggcgc aacgtaagcg
    31021 ggtcgcagca caagaattgg cggaagctga caagattaaa attgtcactg cagctgaagc
    31081 cgaagcagaa aaagaccgcc ttcatggtgt ggggattgcc caacaacgta aggcgattgt
    31141 ggatggattg gcagagtcta tcaccgaact caaggaagcc aatgttggca tgacagaaga
    31201 acaaatcatg tctatcctct tgaccaacca gtatttggat accttgaata cctttgcctc
    31261 taaaggaaat caaactatct ttttaccaaa tacgccaaat ggtgtggatg atatccgtac
    31321 acaaatcttg tcagcccttc gcgctgagaa gaaataatag actaatactc ttcgaaaatc
    31381 tcttcaaacc acgtcagcgt cgccttgccg tataga
//
LOCUS       DAVKZB010000013        29245 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKZB010000013 DAVKZB010000000
VERSION     DAVKZB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.013"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..1005
                     /gene="nanB"
                     /locus_tag="VXA80_000523"
     CDS             <1..1005
                     /gene="nanB"
                     /locus_tag="VXA80_000523"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV0353065.1"
                     /translation="EINGHYYLKLKKNGDNDFRYTVRENGVVYDETTNKPTNYTINDK
                     YEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSLFKVTPTNYIAMTTSQ
                     NRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFATYTSGELTYLISDDSG
                     QTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYMTSRDSGETWSEVSYI
                     DGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQLVVGLVNKEDDSIDWK
                     YHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLSNVVQYIDLEINDLTK
                     "
     gene            1017..2120
                     /locus_tag="VXA80_000524"
     CDS             1017..2120
                     /locus_tag="VXA80_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0353066.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDVCKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            2294..2992
                     /locus_tag="VXA80_000525"
     CDS             2294..2992
                     /locus_tag="VXA80_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0353067.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            3011..4528
                     /locus_tag="VXA80_000526"
     CDS             3011..4528
                     /locus_tag="VXA80_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0353068.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPML
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            4653..5981
                     /locus_tag="VXA80_000527"
     CDS             4653..5981
                     /locus_tag="VXA80_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353069.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            6233..7099
                     /locus_tag="VXA80_000528"
     CDS             6233..7099
                     /locus_tag="VXA80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0353070.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            7115..7954
                     /locus_tag="VXA80_000529"
     CDS             7115..7954
                     /locus_tag="VXA80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0353071.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            8227..8679
                     /locus_tag="VXA80_000530"
     CDS             8227..8679
                     /locus_tag="VXA80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0353072.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEALAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            8776..9120
                     /locus_tag="VXA80_000531"
     CDS             8776..9120
                     /locus_tag="VXA80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353073.1"
                     /translation="MVSSKHAARASFLDGQEITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGIGIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            9404..10048
                     /locus_tag="VXA80_000532"
     CDS             9404..10048
                     /locus_tag="VXA80_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0353074.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            10217..11134
                     /locus_tag="VXA80_000533"
     CDS             10217..11134
                     /locus_tag="VXA80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV0353075.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            11152..12036
                     /locus_tag="VXA80_000534"
     CDS             11152..12036
                     /locus_tag="VXA80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0353076.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(12052..12903)
                     /locus_tag="VXA80_000535"
     CDS             complement(12052..12903)
                     /locus_tag="VXA80_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV0353077.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLEQIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            13103..15160
                     /gene="pbp2b"
                     /locus_tag="VXA80_000536"
     CDS             13103..15160
                     /gene="pbp2b"
                     /locus_tag="VXA80_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV0353078.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            15171..15767
                     /gene="recR"
                     /locus_tag="VXA80_000537"
     CDS             15171..15767
                     /gene="recR"
                     /locus_tag="VXA80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV0353079.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            15943..16986
                     /locus_tag="VXA80_000538"
     CDS             15943..16986
                     /locus_tag="VXA80_000538"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV0353080.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            17070..18443
                     /locus_tag="VXA80_000539"
     CDS             17070..18443
                     /locus_tag="VXA80_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV0353081.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGVHDQILLKGSNSMNLAKLVE
                     SLENEAK"
     gene            18430..19041
                     /locus_tag="VXA80_000540"
     CDS             18430..19041
                     /locus_tag="VXA80_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0353082.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDVEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            19132..19836
                     /locus_tag="VXA80_000541"
     CDS             19132..19836
                     /locus_tag="VXA80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV0353083.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQFKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            20055..21428
                     /gene="ftsA"
                     /locus_tag="VXA80_000542"
     CDS             20055..21428
                     /gene="ftsA"
                     /locus_tag="VXA80_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV0353084.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYATKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            21445..22704
                     /gene="ftsZ"
                     /locus_tag="VXA80_000543"
     CDS             21445..22704
                     /gene="ftsZ"
                     /locus_tag="VXA80_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV0353085.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            22709..23380
                     /locus_tag="VXA80_000544"
     CDS             22709..23380
                     /locus_tag="VXA80_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV0353086.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            23390..23929
                     /locus_tag="VXA80_000545"
     CDS             23390..23929
                     /locus_tag="VXA80_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV0353087.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            23929..24192
                     /locus_tag="VXA80_000546"
     CDS             23929..24192
                     /locus_tag="VXA80_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV0353088.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            24189..24974
                     /locus_tag="VXA80_000547"
     CDS             24189..24974
                     /locus_tag="VXA80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV0353089.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLNK"
     gene            24983..25771
                     /gene="divIVA"
                     /locus_tag="VXA80_000548"
     CDS             24983..25771
                     /gene="divIVA"
                     /locus_tag="VXA80_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV0353090.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            26119..28911
                     /locus_tag="VXA80_000549"
     CDS             26119..28911
                     /locus_tag="VXA80_000549"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV0353091.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLFAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPDLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIVEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         8892 a         5266 c         6434 g         8653 t
ORIGIN      
        1 gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga tttccgttat
       61 acagttagag aaaatggtgt cgtttatgat gaaacaacta ataaacctac aaattatact
      121 ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga acaatattcg
      181 gttgattttg atagtggctc tttaagagaa aaacataatg gaaaacaggt tcctatgaat
      241 gttttctaca aagattcgtt atttaaagtg actcctacta attatatagc aatgacaact
      301 agtcagaata gaggagagag ttgggaacaa tttaagttgt tgcctccgtt cttaggagaa
      361 aaacataatg gaacttactt gtgtcctgga caaggtttag cattaaaatc aagtaacaga
      421 ttgatttttg caacatatac tagtggagaa ctaacctatc tcatttctga tgatagtggt
      481 caaacatgga agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc agaagcacaa
      541 atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac aggtaagata
      601 gcttatatga ctagtagaga ttctggagaa acatggtcgg aagtttcgta tattgatgga
      661 atccaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca attaattgat
      721 ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa gggaggtcaa
      781 ttagttgtcg gtttagtcaa taaagaagat gatagtattg attggaaata ccactatgat
      841 attgatttgc cttcgtatgg ttatgcctat tctgcgatta cagaattgcc aaatcatcac
      901 ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa atgaattgca tttaagcaat
      961 gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag aaaaacatgg
     1021 ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc tacatgcaaa
     1081 aaaatgatgg agcagagatt actcttctct atgatccaga taatgcagag gcgattgcag
     1141 aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc gatgaagtag
     1201 actgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt aaggctgcac
     1261 agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa gattgtcgcg
     1321 agatggtaga tgtgtgtaaa gaaaacaatg taacctttat ggcaggacat attatgaatt
     1381 tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc ggagacgttc
     1441 tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta tcatggaaaa
     1501 aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg gattgcgttc
     1561 aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat gtggcccatg
     1621 aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa ttttctaata
     1681 agcgttttgc tttgttagaa tggggttcag cttatcgttg gggtgaacat tatgtcttaa
     1741 tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga actcttaagc
     1801 tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat gatgatcgga
     1861 ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa ccaggtaaac
     1921 gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg catgagatta
     1981 tggaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa gctgccctag
     2041 aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc aaagtaaaat
     2101 tgtcagaaat tgtaaaataa attttggtat tctcctattt ataggtcgac ttgctcctct
     2161 gaaagtactt ttagaggagc tgtttgactt tgctagtttt tgaaactgaa atctattata
     2221 ctacaaacta ttgaaagcgt tttaatttta aggtataata atctcataga aataaagaaa
     2281 aggaggaaag aggatgccac agattagcaa agaagccttg attgagcaaa tcaaagatgg
     2341 aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt tatacagaag cgggaggggt
     2401 gattcccttg ctggtcaaag cggctgagca aggtggagca gtcggtatcc gagcaaacag
     2461 tgttcgcgat atcaaggaaa ttaaggaggt cactaaactt ccaatcattg ggattatcaa
     2521 acgtgattat ccacctcagg aacccttcat cacggctact atgaaagaag ttgatgaatt
     2581 ggcagaactg gacatcgagg tgattgctct ggattgtacc aagcgtgaac gctacgatgg
     2641 tttggaaatt caagagttca ttcgtcaggt taaggagaaa tatcctaatc agcttttgat
     2701 ggctgatact agtatcttcg aagaagggct agcagctgta gaagcaggaa ttgactttgt
     2761 cggaacaacc ttatcaggct acacatccta cagtccaaaa gtagacggtc cagattttga
     2821 attgattaag aaactctgtg atgccggtgt agatgtcatt gcagaaggaa aaattcatac
     2881 accagaacaa gccaaacaaa tccttgaata tggagtgcga ggcatcgttg ttggtggcgc
     2941 cattactaga ccaaaagaga ttacagaacg cttcgttgct agtcttaaat aagatgtgag
     3001 ggggagtttt atgtttaaag ttttacaaaa agttggaaaa gcttttatgt tacctatagc
     3061 tatacttcct gcagcaggtc tacttttggg gattggtggt gcactttcaa acccaaccac
     3121 gatagcaact tatccaatgc tagacaatag tatttttcaa tcaatattcc aagtaatgag
     3181 ctctgcagga gaggttgtat tcagtaattt gtcactactt ctctgtgtgg gattatgtat
     3241 tggcttagcg aaacgagata aaggaaccgc tgcgttagca ggagtaactg gttacttagt
     3301 tatgactgca acgatcaaag ctttagtaaa actttttatg gcagaaggat ctgcaattga
     3361 tactggagtt attggagcat tagttgtcgg aatagttgcc gtatatttgc acaaccgata
     3421 taacaatatt caattacctt ccgctttagg attctttgga ggttcacgct tcgttcctat
     3481 tgttacatcg ttctcttcta tcttgattgg ctttgtcttc tttgttattt ggccaccttt
     3541 ccaacaactt cttgtttcta caggtggata tatttctcag gcgggtccaa ttggaacttt
     3601 tctatatgga tttttaatga gactttctgg agcagtaggc ttacatcata taatttaccc
     3661 tatgttttgg tatactgaac ttggtggtgt tgaaactgtt gcaggacaaa cagtggttgg
     3721 agctcaaaaa atattttttg ctcaattagc cgatctggcc cattctggat tatttacaga
     3781 aggaacaagg ttttttgcag gtcgtttctc aacaatgatg ttcggtttac cggctgcctg
     3841 tttagcgatg taccatagtg ttcctaaaaa tcgtcgtaaa aaatacgcgg gtttgttttt
     3901 tggagttgct ttaacatctt ttattaccgg tattacagaa ccaattgaat ttatgtttct
     3961 attcgtcagt ccggttctat atgttgttca cgcattcctt gatggtgtta gcttctttat
     4021 tgcagacgtc ttaaatattt caataggaaa cacattttca ggaggtgtaa tcgatttcac
     4081 tttatttgga attttgcagg ggaacgctaa gacgaattgg gttcttcaga ttccatttgg
     4141 acttatttgg agtgttttgt attatattat ttttagatgg ttcattactc aattcaacgt
     4201 tctaacgcca ggacgaggag aagaagtaga ttctaaagaa atttctgaat ccgcagattc
     4261 aacttcaaat actgcagatt atttaaaaca ggatagccta caaattatca gagccttggg
     4321 tggatcaaat aatatagaag atgtagatgc ttgtgtgaca cgtttacgtg tagctgtaaa
     4381 agaagttaat caagttgata aagcactttt aaaacaaatt ggtgcagttg atgtcttaga
     4441 agtgaagggt ggcattcaag caatctatgg agcaaaagca atcttatata aaaatagtat
     4501 taatgaaatt ttaggtgtag atgattaagt acttactgac ttaataaaaa acagaggaga
     4561 gtgatggatg agtaggatga aatgaaatcg catacaagaa ataaagaact cattatccaa
     4621 gttggatacg cttattacat aggagaatac aaatgaaatt tagaaaatta gcttgtacag
     4681 tacttgcggg tgctgcggtt cttggtcttg ctgcttgtgg caattctggc ggaagtaaag
     4741 atgctgccaa atcaggtggt gacggtgcca aaacagaaat cacttggtgg gcattcccag
     4801 tatttaccca agaaaaaact ggtgacggtg ttggaactta tgaaaaatca atcatcgaag
     4861 cgtttgaaaa agcaaaccca gatataaaag tgaaattgga aaccatcgac ttcaagtcag
     4921 gtcctgaaaa aatcacaaca gccatcgaag caggaacagc tccagacgta ctctttgatg
     4981 caccaggacg tatcatccaa tacggtaaaa acggtaaatt ggctgagttg aacgacctct
     5041 tcacagatga atttgttaaa gatgtcaaca atgaaaacat cgtacaagca agtaaagctg
     5101 gagacaaggc ttatatgtat ccgattagtt ctgccccatt ctacatggca atgaacaaga
     5161 aaatgttaga agatgctgga gtagcaaacc ttgtaaaaga aggttggaca actgatgatt
     5221 ttgaaaaagt attgaaagca cttaaagaca agggttacac accaggttca ttgttcagtt
     5281 ctggtcaagg gggagaccaa ggaacacgtg cctttatctc taacctttat agcggttctg
     5341 taacagatga aaaagttagc aaatatacaa ctgatgatcc taaattcgtc aaaggccttg
     5401 aaaaagcaac tagctggatt aaagacaatt tgatcaataa tggttcacaa tttgacggtg
     5461 gggcagatat ccaaaacttt gccaacggtc aaacatctta cacaatcctt tgggcaccag
     5521 ctcaaaatgg tatccaagct aaacttttag aagcaagtaa ggtagaagtg gtagaagtac
     5581 cattcccatc agacgaaggt aagccagctc ttgaatacct tgtaaacgga tttgcagtat
     5641 tcaacaataa agacgacaag aaagtcgctg catctaagaa attcatccag tttatcgcag
     5701 atgacaaaga gtggggacct aaagacgtag ttcgtacagg tgctttccca gtccgtactt
     5761 catttggaaa actttatgaa gacaaacgca tggaaacaat cagcggctgg actcaatact
     5821 actcaccata ctacaacact attgatggtt ttgctgaaat gagaacactt tggttcccaa
     5881 tgttgcaatc tgtatcaaat ggtgacgaaa aaccagcgga tgctttgaaa gccttcactg
     5941 aaaaagcaaa cgaaacaatc aaaaaagcta tgaaacaata gtccttagtt attctataaa
     6001 aagtagtttt ttaaagaacc taagagtgta tacccccttt tccctctaca cagatagtgt
     6061 aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa atgaagttct
     6121 tacataagcg aatcataaaa aatttcattt tgattttaaa acagttcaag aaagtcaaaa
     6181 aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc gtatgcggga
     6241 aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca tctttgtgtt
     6301 ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga ctaaatttga
     6361 gtttgtaggc ttggataact atatccgtat gtttaaagat cctgtcttta caaaatctct
     6421 gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat tctcactctt
     6481 tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc gtttcgtctt
     6541 cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat ggatttatga
     6601 cccactatca gggattctaa actttgtcct taagtcaagc cacatcatca gccaaaacat
     6661 ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc tcttgaccac
     6721 ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg acaattcact
     6781 ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga agattaaatg
     6841 gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta actcattcca
     6901 gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa caagtacctt
     6961 gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg ccaacacaat
     7021 tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta aagtacttgg
     7081 aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa aaaaaccatt
     7141 aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc tgttcatctt
     7201 tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa ttgttattcc
     7261 tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca tggtgcagaa
     7321 ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca tgttcttagt
     7381 ctgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct atggtcaacg
     7441 cattctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg tccttgtacc
     7501 attggtacgt attgtcaact tcatgggaat ccacgatact ctctgggcag ttatcttgcc
     7561 tttgattgga tggccattcg gtgtcttcct catgaaacaa tttagtgaaa atattcctac
     7621 agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct tctggagtgt
     7681 agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct tcatcaatac
     7741 ttggaatgac tacttcatgc aattggtaat gttgacttca cgtaacaatt tgaccatctc
     7801 acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga ttatggcagg
     7861 agctgccctt gctgctgttc caatcgtcac agtcttcctt gtcttccaaa aatccttcac
     7921 acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatcg aatgcaaact
     7981 acgtcagctt caccttgcca tacttaagta ttgcctgcgg ttagcttcct agtttgttct
     8041 tcaattttca ttgagtatag gaaaatcaat ctatcaagat acagaagtat attttataga
     8101 tttagagaat atagaagtta taagtgtcta caaaatggag ggtatgcagt tactttatga
     8161 agttttgtca gacacttatc aacttaagaa tgattttagt taattatcag aaacgaagga
     8221 aagagtatga tttttgacga tttgaaaaac atcacctttt acaaagggat tcatcccaat
     8281 ttagacaaag ctatcgacta cctctaccaa catcgtaaag attcattcga attaggaaag
     8341 tatgagattg atggagataa ggtctttcta gttgttcagg aaaatgtcct caatcaagtt
     8401 gagaataatc aatttgaaca ccataagaac tatgcagatt tgcatttgct gatagaaggg
     8461 catgaatatt cgagctacgg ttcacgtatc aaagacgagg cattagcatt cgacgaagcg
     8521 actgacattg gctttgttca ttgtcatgaa cactacccac tcttgttggg ttatcataat
     8581 tttgcgattt tcttcccagg tgagccacat cagccaactg gctatgcagg tatggaagaa
     8641 aaggttcgaa aatatctctt taaaattttg attgattaaa aataggatga attgtttttt
     8701 tgtaaagctt tgacaatact ctaccatgaa attgatcttt atgaggtaga gaaatgagaa
     8761 taaaatattt aaaaattggt atcttctaag catgctgcaa gagctagttt cttagatgga
     8821 caggaaatta cagttgatga gatgtcctgg ataattaatg gcattgtgaa tgcattgatt
     8881 ggtagataca taaaattagg tacttatgca gctaagtatg gtattggtat agcacgctcg
     8941 atcttaagta gggcagctgc aactgcagca gcaagagtag gattactgac caagatttct
     9001 ggatggattt tacgagtagc tgtgaatgta gctgatgtat atggtaattt cgccaacaat
     9061 attgctgcag cttgggatgc atatgataaa attcctaaca atggtcgtat aaacttttaa
     9121 aatacgagaa tgaaagcact ttgtattttt ttattgaata tgttagcttg gacagtgctt
     9181 gcaatgataa ttcgtggagg gctagatgga tttgataggc atacttggag tactatttta
     9241 attgcttcgc tgttcggggt atatgattat aagcccatag ataaaaatag aaaaaagtcc
     9301 aaaagaaaaa agaaaaaata gatttgttca tggtagggac ttatgaaagc tttactgaca
     9361 aaaaagaaaa cagtttacaa agaaaaatga tggaggagca aacatggcac aaaaaggagt
     9421 aagccttatc aaggcagcat ttgatacaga taactttctc atgcgtttta gtgagaaggt
     9481 cttggacatc gtgacagcca atcttctttt tgtcgtctct tgtttaccca tcgtgacgat
     9541 tggagtggct aaaatcagcc tctacgagac catgttcgaa gttaagaaga gcagacgggt
     9601 gcctgttttt aaaatctatc taagatcttt caagcaaaat ctgaaactag gtcttcagct
     9661 gggtttaatg gagttaggaa ttgtgtttct taccctttca gatctctatc ttttctgggg
     9721 tcaaacagct ctgcccttcc aattgctgaa agccatttgt ttaggtattc tgatttttct
     9781 tactatcgtg atgctggcta gttaccctat cgcggcacgt tatgacctat cttggaaaga
     9841 aattcttcaa aaaggattga tgttggctag ttttaacttt ccttggttct tcctcatgtt
     9901 agccattctt gtcctcattg tgatggttct ttatctgtcc gccttcagtc tactcttagg
     9961 tggctcagtc ttcctacttt ttgggtttgg actattggtc tttatccaga ctggattgat
    10021 ggagaaaatt ttcgcaaaat accaatagga gctttatttc tgaaactact ttcaaaggct
    10081 ccaaacgcta ttctataagc gagaaactaa aatctaagtg tctataagat attgaaagcc
    10141 ttttcggtaa agtgtatact aaagaagtaa agaaaaatct gctttagcag aaaataaaaa
    10201 taataggaga aaatctatgt cagatttgaa aaaatacgaa ggtgtcattc cagccttcta
    10261 cgcatgttat gatgagcaag gagaagtaag tccagagcgt acgcgtgcct tggttcaata
    10321 cttcattgat aaaggtgttc aaggtcttta tgtcaatggt tcttctggtg aatgtatcta
    10381 ccaaagcgtt gaagatcgca agttgatttt ggaagaagtc atggcggtag ctaaaggtaa
    10441 attgaccatt attgcccatg ttgcctgcaa caatactaaa gacagtatgg aacttgctcg
    10501 ccatgctgaa agcttgggag tagatgctat tgcaacgatt ccaccaattt acttccgctt
    10561 gccagaatac tcagttgcca aatactggaa cgatattagt tctgcagctc caaacacaga
    10621 ctacgtgatt tacaacattc ctcaattggc aggggttgct ttgactccaa gcctttacac
    10681 agaaatgttg aaaaatcctc gtgttatcgg tgttaagaac tcttctatgc cagttcaaga
    10741 tatccaaacc tttgtcagcc ttggcgggga agaccatatt gtctttaacg gtccagatga
    10801 acagttccta ggtggccgcc tcatgggtgc tagggctggt atcggtggta cttatggtgc
    10861 tatgccagaa ctcttcttga aactcaatca gttgattgcg gataaggacc tagaaacagc
    10921 gcgtgaattg cagtatgcta tcaacgcaat cattggtaaa ctcacttctg ctcatggaaa
    10981 tatgtacggt gtcatcaaag aagttttgaa aatcaatgaa ggcttgaata ttggatctgt
    11041 tcgttcacca ttgacaccag tgactgaaga agatcgtcca gttgtagaag cggctgcagc
    11101 cttgattcgt gaaaccaagg agcgcttcct ctaatctaaa aggaggtatt tatgacatac
    11161 tacgttgcaa ttgatatcgg tggaactaac atcaagtatg gtttggttga tcaagagggg
    11221 caacttcttg aatcgcatga aatgccaact gaggcgcata agggtggacc tcatatctta
    11281 caaaagacca aagatatcgt agctagttat ttagaaaaag gcccagtagc aggtgttgcc
    11341 atatcttctg ctgggatggt ggatccggat aagggtgaga ttttctatgc tgggccgcaa
    11401 atccctaact acgcaggcac ccagttcaaa aaggaaatcg aagaaagctt tactattcct
    11461 tgtgagattg aaaatgatgt caactgtgca ggtcttgctg aggcagtatc tggttcaggc
    11521 aagggagcaa gtgtgacact ttgcttgacc attggaacag gtatcggtgg ttgcttgatt
    11581 atggatagga aagtcttcca tggttttagt aattcagcct gtgaagtcgg gtatatgcat
    11641 atgcaggatg gagcttttca agacttggct tctacaacag ctttagtgaa atatgtagct
    11701 gaagcccatg gagaagatgt tgatcagtgg aatggccgac gtatctttaa ggaagctact
    11761 gaaggaaaca aaatctgcat ggaaggtatt gaccgtatgg ttgactatct aggaaaaggt
    11821 ctggcaaata tttgctacgt tgccaatcca gaagtggtta ttcttggtgg tggtatcatg
    11881 gggcaagagg ctatcctcaa acctaagatc cgcacagcct tgaaagaggc tttggtacca
    11941 agtttagcag aaaaaacacg attagaattt gcccatcacc aaaatacagc agggatgttg
    12001 ggtgcatatt atcattttaa gacaaaacaa tcctagtttg gctcagccaa actaggattt
    12061 tcttacacgt ttttgtctac gatagccgtt gagtttttta ttttcccagt agctattaaa
    12121 gattttttcc ttgctttcgc gattgatttc caaaaagtag gcataaatca aatcgataaa
    12181 gaagagcata ggaagttgag cggatattcg ttggatatag gagggttggc tgtgggtggc
    12241 tacaagaaca gtctctgtat aggtctggct atctttattg ggaacacttg taaagagtac
    12301 agtctttgcc cccatctcct tggcatctaa tagactatct aaaatagaag gagttgagcc
    12361 tgaaagtgag aagcccagta ctagacaatt ttcatccatg atgctggttg tccaggcaaa
    12421 gccgtcttgg tctgtcaaag cttcgcagac cacacctagt cgcataaaac gtaatttcat
    12481 ttcacgggcg acgaggccag aactccctgt tccaaagaag tagatacgct cagcatcttc
    12541 gattagctgg gcaatttgct ctagttggat ttcgtcaatc aagtcttgtg tttgttccct
    12601 catattgcta taacttctga ggactcgttt ggtcagtgga ctatgcttgg agacttggtt
    12661 ggcttgattt tctgcctgat gttggtattg gaaaataaat tctcggtagc cagtaaagcc
    12721 acacttttta gcaaagcggg tcaaagcagc ttgagaaata tgtaattttt gggtgacttg
    12781 ttgagaagat aaatcatctg taatcgtttc agcttgcaaa aaatagcgag cgatttcttg
    12841 ctctaggtct gtcatttctt caaaatgtga atcaatgata gttgcgatat ctggtttgtc
    12901 catagggaaa gctcctttac atgagtcata ctggaagact agatcaggga atagtcacac
    12961 ttcattataa cacataatat aaggatagat aaataaaaac gcatctctgt tttaaaaacg
    13021 aaaaaatcga aaaagcttct ctcttttcca taattttcta ctcaaattgt ggtacaatta
    13081 agagtaagat tttaagttag aaatgagact gatttgtatg agaaaattta acagccattc
    13141 gattccgatt cggcttaatt tattgttttc aatcgtcatt ttactcttta tgaccattat
    13201 tggtcgtttg ttgtatatgc aggttttgaa caaggatttt tacgaaaaaa agctagcctc
    13261 agctagtcag accaagatta caagcagttc agcccgtggg gaaatttatg atgctagtgg
    13321 aaaacctttg gtagaaaata cgttaaagca ggttgtttcc tttacgcgta gcaataaaat
    13381 gacggctaca gacttaaaag aaacagctaa aaagttactg acttatgtga gcatcagttc
    13441 tccaaatttg acagaacgcc agctggcgga ttactatttg gctgatcctg aaatctataa
    13501 aaaaatagtg gaagctctcc caagcgagaa acgcttggat tcagatggca atcgtctatc
    13561 cgaatcagaa ctgtataaca atgcggtcga tagtgtacaa acgagtcaac taaactatac
    13621 agaggatgaa aagaaagaaa tctatctttt tagtcagtta aatgctgttg gaaactttgc
    13681 gacaggaacc attgcgacag atcctctaaa tgattctcag gtggctgtta ttgcctctat
    13741 ttcaaaggag atgcctggca ttagtatttc tacttcttgg gatagaaagg ttttggaaac
    13801 ttccctttct tctatagttg ggagtgtatc cagtgaaaaa gctggtctcc cagcggaaga
    13861 agcagaagcc tatcttaaaa aaggctattc tctaaatgat cgtgtaggaa cctcctattt
    13921 ggaaaagcaa tatgaagaga ccttacaagg aaaacgctcg gtaaaagaaa tccatctgga
    13981 taaatatggc aatatggaaa gcgtggatac aattgaggaa ggtagtaagg gaaacaatat
    14041 caaactgacc attgatttgg ccttccaaga tagcgtggat gctttactga aaagttattt
    14101 caattccgag ctagaaaatg gtggagccaa gtattctgaa ggtgtctatg cagtcgccct
    14161 taacccaaaa acaggtgcgg ttttgtctat gtcagggatt aaacatgact tgaaaacggg
    14221 agagttgacg cctgattcct tgggaacggt aaccaatgtc tttgttccag gttcggttgt
    14281 caaggcggcg accatcagct caggttggga aaatggagtc ttgtcaggaa accagacctt
    14341 gacagaccag tccattgtct tccaaggttc agctcccatc aattcttggt atactcaggc
    14401 ttacggttca ttccctatca cagcggtcca agctctggag tattcatcta atacctatat
    14461 ggtccaaaca gccttaggtc ttatggggca aacctatcaa cccaatatgt ttgtcggcac
    14521 cagcaatcta gagtctgcta tggagaaact gcgttcaacc tttggcgaat atggcttggg
    14581 tactgcgaca ggaattgacc taccagatga atctactgga tttgttccca aagagtatag
    14641 ctttgctaat tacattacta atgcctttgg gcagtttgat aactatacgc cgatgcagtt
    14701 ggctcagtat gtagcaacta ttgcaaataa tggtgttcgt gtggctcctc gtattgttga
    14761 aggcatttat ggtaataatg ataagggagg actgggtgac ttgattcagc aactgcaacc
    14821 gacagagata aataaggtca atatatccga ctccgatatg agcatcttgc accaaggttt
    14881 ttatcaggtt gcccatggta ctagtggatt gacaactgga cgtgcctttt caaatggtgc
    14941 cttggtatcc attagcggaa aaacaggtac agccgaaagc tatgtggcag atggtcagca
    15001 agcaaccaat accaatgcgg tggcctatgc cccatctgat aatccccaaa tcgctgtcgc
    15061 agtggtcttt cctcataata ccaatctaac aaatggtgta ggaccttcca ttgcgcgtga
    15121 cattatcaat ctgtatcaaa aataccatcc aatgaactag aaaggaaatt atgctttatc
    15181 caacacctat tgccaagttg attgacagtt attctaagtt accaggtatc gggattaaga
    15241 cggctacgcg tctggccttt tatacgattg ggatgtctgc tgatgatgtc aatgaatttg
    15301 caaaaaatct cctttctgct aagagagaat tgacatattg ttctatttgt ggacgtttga
    15361 cagacgacga tccttgttct atctgtactg atccgactcg tgaccagaca acaattttag
    15421 ttcttgagga tagtagagat gtggcagcca tggaaaatat ccaagaatac catggactct
    15481 atcatgtcct gcatggcctc atttctccta tgaatggtat cagtccggac gatatcaatc
    15541 tcaagagcct tatgactcgt cttatggata gtgaggtttc agaagtgatt gtggcgacta
    15601 atgctacagc ggatggtgaa gcgacttcca tgtatctttc acgtttgctc aagccggctg
    15661 gtatcaaggt tacgcgtcta gcacgaggtc tcgctgtggg agcggacatt gagtatgcgg
    15721 acgaagtgac actcttacga gcgattgaaa atcggacaga gttgtaagtg taggcaaatt
    15781 tacgaactcc cttcatttat aaaaaatcaa agaggctgaa aatcgttcct atcggcctct
    15841 ttttgtatag tgtgatgagt aggatcgggt tcaagtttta aaaaaccaag caaatatgat
    15901 atactaaaga gcgagtattc tagtagaatt aggacaaata atatgaaaca aacgattatt
    15961 cttttatatg gtggacggag tgcggaacgc gaagtctctg tcctttcagc tgagagtgtc
    16021 atgcgtgcgg tcaattacga ccgtttcaca gtcaagactt tctttatcag tcagtcaggt
    16081 gactttatca aaacacagga atttagtcat gctccggggc aagaagaccg tctcatgacc
    16141 aatgaaacca ttgattggga taagaaagtt gcaccaagtg ctatctacga agaaggtgca
    16201 gtggtctttc cagtccttca cgggccaatg ggagaagatg gctctgttca aggattcttg
    16261 gaagttttga aaatgcctta cgttggttgc aacattttgt catcaagtct tgccatggat
    16321 aaaatcacga ctaagcgtgt tctggaatct gctggtattg cccaagttcc ttatgtggct
    16381 atcgttgaag gcgatgatgt gactgctaaa atcgctgaag tggaagaaaa attggcttat
    16441 ccagtcttca ctaagccgtc aaacatgggg tctagtgtcg gtatttctaa gtctgaaaac
    16501 caagaagaac tccgtcaagc cttaaaactt gccttccgat atgacagccg tgtcttggtt
    16561 gagcaaggag tgaatgcccg tgaaattgag gttggcctct tgggtaacta cgatgtcaag
    16621 agcacgctac ctggagaagt tgtcaaggac gttgcctttt atgactacga tgccaagtat
    16681 attgataaca agattactat ggatattcct gccaaaatca gtgatgatgt ggtggctgtc
    16741 atgcgtcaaa atgcagaaac agccttccgt gccattggtg gccttggtct atctcgttgc
    16801 gatttcttct atacagataa gggagagatt tttctcaacg agctcaatac catgccaggt
    16861 tttacccagt ggtctatgta cccactactt tgggacaata tggggatcag ctacccagaa
    16921 ctaatcgagc gtttggttga ccttgccaag gaaagctttg acaagcgcga agcgcatttg
    16981 atataaaatg aaagagaggg tagaagccag aaccatcact gcacggtgac tagagttctc
    17041 ggacttcagc cctttttaaa ggagtagaaa tgaaattaac aatccatgaa attgcccaag
    17101 ttgttggagc caaaaatgat atcagtatct ttgaggacac ccagttagaa aaagctgagt
    17161 ttgatagtcg tttgattgga actggagatt tatttgtgcc acttaaaggt gcgcgtgatg
    17221 gccatgactt tattgaaaca gcctttgaaa atggtgcagc agtaaccttg tctgagaaag
    17281 aggtctcaaa tcatccttac attttagtag atgatgtttt gacagccttt caatccctag
    17341 catcctacta tcttgaaaaa acgactgttg atgtctttgc tgttacaggt tcaaatggca
    17401 agacaacgac taaggatatg ttggcgcatt tactgtcaac aagatacaag acctacaaaa
    17461 cacaaggcaa ttacaataat gagattggcc ttccttacac agttctccac atgcctgaag
    17521 gaacagaaaa gttggtcttg gagatggggc aggaccactt gggagatatt catctcttgt
    17581 ctgaattggc tcgtccaaaa acagccatcg tgaccttggt tggagaagct catttggcct
    17641 ttttcaaaga tcgttctgag attgctaaag gaaaaatgca aattgcagac ggaatggctt
    17701 caggttcctt gcttttaacg ccggctgacc ctatcgtaga ggactatttg ccaactgata
    17761 aaaaggtggt ccgttttggg caaggggcag agttggaaat cacagacttg gttgagcgta
    17821 aggacagtct gacctttaag gctaatttct tggaacaagt ccttgatttg ccagtaactg
    17881 gcaagtacaa tgcgacaaat gctatgattg catcctatgt tgccttgcaa gaaggagttt
    17941 cagaggagca aattcatcag gccttccaag gtcttgaatt gacgcgtaat cgtactgagt
    18001 ggaagaaagc agccaatgga gcagatatcc tgtcagacgt atataatgcc aatccaactg
    18061 ctatgaagct gattttagag actttctctg ccattccagc caatgaaggt ggcaagaaaa
    18121 ttgcagtgtt ggcggatatg aaggagcttg gtgaccagtc tattcaactc cataaccaga
    18181 tgattttgag cctttctcca gatgtgcttg ataccgttat tttctatgga gaaaatattg
    18241 ctgaattagc ccaattggct agtcaaatgt tcccaatagg ccacgtttac tacttcaaga
    18301 aaacagaaga ccaagaccaa tttgaagacc tagtcaagca ggtcaaagaa agccttggag
    18361 tccacgacca aatcctgctc aaaggttcta actctatgaa tctagccaaa ttggtagaaa
    18421 gtttagaaaa tgaagccaag tgattttgtt aagtatctgc aaagaatgat tgcccttaca
    18481 gatactggct taacctttac aaaggatcct tttgaccgtg agcgctacga ggacttgcga
    18541 agtctgttat ctgaaatgtt gaatcaggga tcagacctgg atgtagaaga agtagcagaa
    18601 gtcttgaaac caacttcagc ttatgcgact cccttaatgg acgtccgtgc ttggattgtt
    18661 gaggatgaga agatttgtct ggttagggga caaggagagg atagttgggc tttgccagga
    18721 ggctttggtg aagtcggcta ttctccaact gaaaatattc tcaaggaaat tgaagaagaa
    18781 accggtttta aagccaaagt tgaaagactg ctagctgttt ttgataccaa tcgtttccaa
    18841 ctacagagca aacaatatgc aaagtttgtt tttgaatgta agcttctcga tggacaattc
    18901 caagaaaatc aagaaatcgc tgaccttcaa ttttttgcaa ttgaccaact gccgaattta
    18961 tctgaaaaac gcattaccaa ggagcaaatc gagattcttt ggcaggttta tcaaggtcag
    19021 agggagcaat atcttgacta agaagatgat tatcgtattt ctaaatccat ttttgacaac
    19081 tagcatggta taataatatg caggaaaatt ttgaattatg aggaagacta gatgaattta
    19141 tgggatattt tctttacgac tcaggcaacc gagccgccca aatttgacct tttttggtat
    19201 gttagcctat ttacgctctt agccttaacc ttttatacag cccatcgcta tcgtgaaaag
    19261 aaggtttacc aacgattttt ccaaatcttg cagactgttc agttaatcct tctttatggt
    19321 tggtactggg ttaatcatat gccactgtca gaaagcctac ccttttacca ttgccgtatg
    19381 gctatgtttg tggtactctt gcttcctggt cagttcaaat ataaacaata ctttgcatta
    19441 ttgggaacat ttgggacatt agcagccttt gtttatccag tgccagatgc ttaccctttt
    19501 ccacatatta ccattttatc ctttatcttt ggtcatttag cactcttggg gaactctcta
    19561 gtttatctat tgagacagta taatgcgcga ttgctggatg tgaagggaat ttttctcatg
    19621 acctttgccc taaatgcctt gatttttgtg gtcaatttgg tgacaggtgg cgattacgga
    19681 tttttgacaa aaccgccatt ggttggggat cacggtctag tagctaatta tttacttgtt
    19741 tcaattgtgc tggtagctac tatcagtttg actaagaaaa tcttagaatt ctttttagct
    19801 caagaagcag aaaaaatgat tgcaaaggaa gcttaacaca gagctttctt ttttgctctt
    19861 agagagtttt tacaagcagc ttataaaata agaatttctg aatagacaaa ctcaaaaaat
    19921 ggctgggaaa tttaggaaaa aagcaagcac gattaaattt tttgtgttat aatattttgt
    19981 gaatagctat gcctatgttt agctatggaa taatacgaag tgcgaaactt ggaagataga
    20041 gaggaagcga tgtaatggct agagaaggct tttttacagg tctagatatt ggaacaagct
    20101 ctgtcaaggt gcttgtggcc gagcagagaa atggtgaatt aaatgtaatt ggcgtgagta
    20161 atgccaaaag taaaggtgta aaggatggaa ttattgttga tattgatgca gcagcaactg
    20221 ctatcaagtc agccatttcc caagcggaag aaaaggcagg catttcgatt aaatcagtaa
    20281 atgtcggctt gcctggtaat cttttgcagg tagaaccaac tcaggggatg attccagtaa
    20341 catctgatac taaggaaatt acggatcaag atgttgaaaa tgttgtcaaa tcagctttga
    20401 caaagagtat gacacctgac cgtgaagtca ttacctttat tcctgaagaa tttattgtgg
    20461 atggtttcca agggattcgt gacccacgtg gcatgatggg ggttcgcctt gaaatgcgtg
    20521 gtttgcttta tacaggacct cgtactatct tgcacaattt gcgtaagacg gttgagcgtg
    20581 caggtgttca ggttgaaaat gttatcattt caccactagc aatggttcaa tctgttttga
    20641 acgaagggga acgtgaattt ggtgctacag tgattgatat gggggcaggt caaacgactg
    20701 tcgctacaat ccgtaatcaa gaactccagt tcacacatat tctccaagaa ggtggagatt
    20761 atgcaactaa agatatctcc aaggttttga aaacctctcg caaattagcg gaaggcttga
    20821 aactgaatta cggggaagcc tatccgcctc ttgcaagcaa agaaaccttc caagtagagg
    20881 ttattggaga agtagaagca gtcgaagtga cggaagccta cttgtcagaa attatttctg
    20941 cacgaatcaa gcacatcctt gaacaaatca agcaagaatt agatagaagg cgtctattgg
    21001 acctccctgg tggtattgtc ttaatcggtg gaaatgccat tttaccaggt atggttgagc
    21061 ttgctcagga agtctttggc gtccgtgtca agctttatgt tccaaatcaa gttggtatcc
    21121 gtaatccagc ctttgcgcat gtgattagtt tatcagaatt tgcgggtcaa ttaacagaag
    21181 ttaatctttt ggctcaggga gcgataaaag gtgagaatga cttaagtcat cagccaatta
    21241 gttttggtgg gatgctgcaa aaaacagctc agtttgtaca atcaacgcct gttcaaccag
    21301 ctcctgctcc agaagtagag ccggtggcgc ctacagaacc aatggcggat ttccaacaag
    21361 cttcacaaaa taaaccgaaa ttagcagatc gtttccgtgg cttgatcgga agcatgtttg
    21421 acgaataaag aggaaaaata aattatgaca ttttcatttg atacagctgc tgctcaaggg
    21481 gcagtgatta aagtaattgg tgtcggtgga ggtggtggca atgccatcaa ccgtatggtc
    21541 gacgaaggtg ttacaggcgt agaatttatc gcagcaaaca cagatgtaca agcattgagt
    21601 agtacaaaag ctgagactgt tattcagttg ggacctaaat tgactcgtgg tttgggtgca
    21661 ggaggtcaac ctgaggttgg tcgtaaagcc gctgaagaaa gcgaagaaac actgacggaa
    21721 gctattagtg gtgccgatat ggtcttcatc actgctggta tgggaggagg ctctggaact
    21781 ggagctgctc ctgttattgc tcgtatcgcc aaagatttag gtgcgcttac agttggtgtt
    21841 gtaacacgtc cctttggttt tgaaggaagt aagcgtggac aatttgctgt agaaggaatc
    21901 aatcaacttc gtgagcatgt agacactcta ttgattatct caaacaacaa tttgcttgaa
    21961 attgttgata agaaaacacc gcttttggag gctcttagcg aagcggataa cgttcttcgt
    22021 caaggtgttc aagggattac cgatttgatt accaatccag gattgattaa ccttgacttt
    22081 gccgatgtga aaacggtaat ggcaaacaaa gggaatgctc ttatgggtat tggtatcggt
    22141 agtggagaag aacgtgtggt agaagcggca cgtaaggcaa tctattcacc acttcttgaa
    22201 acaactattg acggtgctga ggatgttatc gtcaacgtta ctggtggtct tgacttaacc
    22261 ttgattgagg cagaagaggc ttcacaaatt gtaaaccagg cagcaggtca aggagtgaac
    22321 atctggctcg gtacttcaat tgatgaaagt atgcgtgatg aaattcgtgt aacagttgtt
    22381 gcaacgggtg ttcgtcaaga ccgcgtagaa aaggttgtgg ctccacaagc tagatctgct
    22441 actaactacc gtgagacagt gaaaccagct cattcacatg gctttgatcg tcattttgat
    22501 atggcagaaa cagttgaatt gccaaaacaa aatccacgtc gtttggaacc aactcaggca
    22561 tctgcttttg gtgattggga tcttcgccgt gaatcgattg ttcgtacaac agattcagtc
    22621 gtttctccag tcgagcgctt tgaagcccca atttcacaag atgaagatga attggataca
    22681 cctccatttt tcaaaaatcg ttaagtaaat gaatgtaaaa gaaaatacag aacttgtttt
    22741 tcgagaagtt gcagaggcta gtctgagtgc tcatcgagag agtggttcgg tctctgtcat
    22801 tgcagttacc aagtatgtag atgtaccgac agcggaagcc ttgcttccgc taggtgttca
    22861 tcatatcggt gaaaatcgtg tagataagtt tctggaaaaa tatgaagctt taaaagatcg
    22921 agatgtgact tggcatttga ttggtacctt gcaaagacgt aaggtgaaag atgtcattca
    22981 atacgttgat tatttccatg cattggactc agtaaagcta gcaggggaaa ttcaaaaaag
    23041 aagtgaccga gtcatcaagt gtttccttca agtaaatatt tctaaagaag aaagcaaaca
    23101 cggtttttcg agagaggaac tgctggaaat cttgccagag ttagccagac tagataagat
    23161 tgaatatgtt ggtttaatga cgatggcacc ttttgaggct agcagtgagc agttgaaaga
    23221 gattttcaag gcggcccaag atttacaaag agaaattcaa gagaaacaaa ttccaaatat
    23281 gcctatgacc gagttaagta tgggaatgag tcgtgattat aaagaagcga ttcaattcgg
    23341 ttccactttt gttcgtatag gtacatcatt ttttaagtag gagagaacca tgtctttaaa
    23401 agatagattc gatagattta tagattattt tacggaggat gaggattcaa gtctccctta
    23461 tgaaaaaaga gatgagcctg tgtttactcc agtaaattct tcacaggaac cggctctccc
    23521 aatgaatcaa ccttcacagt cggctggtac aaaagagaac aatatcacca gacttcatgc
    23581 aagacaacag gaattggcaa atcagagtca gcgtgcaacg gataaggtca ttatagatgt
    23641 tcgttatcct agaaaatatg aggatgcaac agaaattgtt gatttattgg caggaaacga
    23701 aagtatcttg attgattttc agtatatgac agaggtgcag gctcgtcgtt gtttggacta
    23761 tttggatggg gcttgtcatg ttttagctgg aaatttgaaa aaggtagctt ctaccatgta
    23821 tttgttgaca ccagtgaacg ttattgtaaa tgttgaagat atccgtttac cagatgaaga
    23881 tcaacagggt gagttcggtt ttgatatgaa gcgaaataga gtacgataat gattttttta
    23941 attcgtatga tttataatgc agtggatatt tactccctaa ttttggtagc cttcgctgtc
    24001 atgtcttggt ttccaggtgc ctacgaatcc agtttaggtc gttggattgt agcgttggta
    24061 aaaccagtgc ttgctccctt gcaacgcctg cctttacaga tagcgggtct tgatttatct
    24121 gtttgggttg cgattgtttt ggttcgattt ttaggagaaa acctagtgcg ttttctggcg
    24181 atgataggat gaataaaggg atttatcagc atttctccat agaagatcgt ccatttcttg
    24241 acaagggaat ggaatggata aagaaggtag aagatagcta tgctcctttt ttaactcctt
    24301 ttatcaatcc tcatcaggag aagctattaa agattttggc caaaacctat ggtcttgctt
    24361 gtagcagtag tggggaattc gtctcgagtg agtatgttcg agttttatta tacccagatt
    24421 atttccaacc agagttttca gattttgaaa tatctctcca ggaaattgtg tattccaata
    24481 aatttgaaca tttaacgcat gctaagattt tagggacagt catcaatcaa ttagggattg
    24541 aacggaaact ttttggagat atcctagtag atgaagaacg ggcgcagatt atgattaatc
    24601 agcagtttct tcttctcttt caagatggac taaagaaaat tggtcgtata cctgtttcgc
    24661 tggaggaacg tcctttcacc gagaaaatag ataagctaga acagtatcga gaactggatt
    24721 tatctgtgtc tagttttcga ttagatgttc ttttatcaaa tgttttgaaa ctatctagga
    24781 atcaagcaaa ccagttgatt gaaaagaaac ttgtccaagt aaattatcat gtggtagaca
    24841 aatcagatta cactgttcaa gttggcgact tgattagtgt gagaaaattt ggtcgcttga
    24901 gattacttca agataaggga caaacgaaaa aagagaagaa aaaaataacc gtccagttat
    24961 tattaaataa gtgaggaata gaatgccaat tacatcatta gaaataaagg acaagacttt
    25021 tggaactcga ttcagaggtt ttgatccaga agaagtcgat gaatttttag atattgtggt
    25081 tcgtgattac gaagatcttg tgcgtgcgaa tcatgataaa aatttgcgta ttaagagttt
    25141 agaagagcgt ttgtcttact ttgatgaaat aaaagattca ttgagccagt ctgtattgat
    25201 tgctcaggat acagctgaga gagtgaaaca ggcggcgcat gaacgttcaa acaatatcat
    25261 tcatcaagca gagcaagatg cgcaacgctt gttggaagaa gctaaatata aggcaaacga
    25321 gattcttcgt caagcaactg ataatgctaa gaaagtcgct gttgaaacag aagaattgaa
    25381 gaacaagagc cgtgtcttcc accaacgtct caaatctaca attgagagtc agttggctat
    25441 tgttgaatct tcagattggg aagatattct ccgtccaaca gctacttatc ttcaaaccag
    25501 tgatgaagcc tttaaagaag tggttagcga agtacttgga gaaccgattc cagctccaat
    25561 tgaagaagaa ccaattgata tgacacgtca gttctctcaa gcagaaatgg aagaattaca
    25621 agctcgtatt gaggtagccg ataaagaatt gtctgaattt gaagctcaga ttaaacagga
    25681 agtggaaact ccaactcctg tagtgagtcc tcaagttgaa gaagagcctc tgctcatcca
    25741 gttggcccaa tgtatgaaga accagaagta gctccagtgc atccgacagg tccaacacca
    25801 gctacagaaa ctgttgattc agcaccggga tttgaagcac cgcaagaatc tgttacaatt
    25861 ttataagaaa tattctgaga acaatatctt atccttatat tcccagcgag caggagatgg
    25921 tgtgagtcct gcattcccta tcgataagat tatcctctca aaaactcaag tctgaagcta
    25981 gtaagatttg acgttcccca cgttacggga taagagggag aaagactaaa tctttttccg
    26041 aataaaggtg gtaccacgat tttcgtcctt tttggaagtc gtggttttta atttgttatt
    26101 atttataaag gagataccat gaaactcaaa gacaccctta atcttgggaa aactgaattc
    26161 ccaatgcgtg caggccttcc taccaaagag ccagtttggc aaaaggaatg ggaagatgca
    26221 aaactttatc aacgtcgtca agaattgaac caaggaaaac ctcatttcac cttgcatgat
    26281 ggccctccat acgctaacgg aaatatccac gttggacatg ctatgaacaa gatttcaaaa
    26341 gatatcattg ttcgttctaa gtctatgtca ggattttacg caccatttat tcctggttgg
    26401 gatactcatg gtctgccaat cgagcaagtc ttgtcaaaac aaggtgtcaa acgtaaagaa
    26461 atggacttgg ttgagtactt gaaactttgc cgtgagtacg ctctttctca agtagataaa
    26521 caacgtgaag attttaaacg tttgggtgtt tctggtgact gggaaaatcc atatgtgacc
    26581 ttgactcctg actatgaagc agcccaaatc cgtgtctttg gtgagatggc taataagggt
    26641 tatatctacc gtggtgccaa gccagtttac tggtcatggt catctgagtc agcccttgct
    26701 gaagcagaga ttgaatacca tgacttggtt tcaacttccc tttactatgc caacaaggta
    26761 aaagatggca aaggagttct agatacagat acttatatcg ttgtctggac aacgactcca
    26821 tttaccatca cagcttctcg tggtttgacg gttggtgcag atattgatta cgttttggtt
    26881 caacctgctg gtgaagctcg taagtttgtc gttgctgctg aattattgac tagcttgtct
    26941 gagaaatttg gctgggctga tgttcaagtt ttggaaactt accgtggcca agaactcaac
    27001 cacatcgtaa cagaacaccc atgggataca gctgtagaag agttggtaat tcttggtgac
    27061 cacgttacga ctgactctgg tacaggtatt gtccatacag cccctggttt tggtgaggac
    27121 gattacaatg ttggtattgc taataatctt gaagtcgcag tgactgttga tgaacgtggt
    27181 atcatgatga agaatgctgg tcctgaattt gaaggtcaat tctatgaaaa ggtagttcca
    27241 actgttattg aaaaacttgg taacctcctt tttgcccaag aagaaatctc tcactcatat
    27301 ccatttgact ggcgtactaa gaaaccaatc atctggcgtg cagttccaca atggtttgcc
    27361 tcagtttcta aattccgtca agaaatcttg gacgaaattg aaaaagtgaa attccactca
    27421 gaatggggta aagtccgtct ttacaatatg atccgtgacc gtggtgactg ggttatctct
    27481 cgtcaacgtg cttggggtgt tccacttcct atcttctacg ctgaagatgg tacagctatc
    27541 atggtagctg aaactattga acacgtagct caactttttg aagaacatgg ttcaagcatt
    27601 tggtgggaac gtgatgccaa agacctcttg ccagaaggat ttactcatcc aggttcacca
    27661 aacggcgagt tcaaaaaaga aactgatatc atggacgttt ggtttgactc aggttcatca
    27721 tggaatggag tggtggtaaa ccgtcctgac ttgacttacc cagccgacct ttacctagaa
    27781 ggttctgacc aataccgtgg ttggtttaac tcatcactta tcacatctgt tgccaaccat
    27841 ggcgtagcac cttacaaaca aatcttgtca caaggttttg cccttgatgg taaaggtgag
    27901 aagatgtcta aatctcttgg aaatactatt gctccaagcg atgttgaaaa acaattcggt
    27961 gctgaaatct tgcgtctctg ggtaacaagt gttgactcaa gtaatgacgt gcgtatctct
    28021 atggatatct tgagccaagt ttctgaaact taccgtaaga ttcgtaacac tcttcgtttc
    28081 ttgattgcca atacatctga ctttaaccca gctcaagata cagtcgctta cgatgagctt
    28141 cgttcagttg ataagtacat gacgattcgc tttaaccagc ttgtcaagac cattcgtgat
    28201 gcctatgcag actttgaatt cttgacgatc tacaaggcct tggtgaactt tatcaacgtt
    28261 gacttgtcag ccttctacct tgattttgcc aaagatgttg tttacattga aggtgccaaa
    28321 tcactggaac gccgtcaaat gcagactgtc ttctatgaca ttcttgtcaa aatcaccaaa
    28381 ctcttgacac caatccttcc tcacactgcg gaagaaatct ggtcatatct tgagtttgaa
    28441 acagaagact tcgtccaatt gtcagaatta ccagaagttc aaacttttgc taaccaagaa
    28501 gaaatcttgg atacatgggc agccttcatg gactttcgtg gacaagcaca aaaagccttg
    28561 gaagaagctc gtaatgcaaa agttatcggt aaatcacttg aagcacactt gacagtttat
    28621 ccaaatgaag ttgtgaaaac tctactcgaa gcagtaaaca gcaatgtagc tcaacttttg
    28681 atcgtgtctg agttgaccat cgtagaagga ccagctccgg aagctgccct tagcttcgaa
    28741 gatgtagcct tcacagttga acgtgctact ggtgaagtat gtgaccgttg ccgtcgtatc
    28801 gacccaacaa cagcagaacg cagctaccag gcagttatct gtgaccactg tgcaagcatc
    28861 gtagaagaaa actttgcgga agcagtcgca gaaggatttg aagagaaata agattgaaaa
    28921 gtctaggcaa aattcaattt gagaagaaaa gacaactaat tttatagtct attaaacgca
    28981 ttgtatcacg tttttgaata cctgatatga tgcgtttttt atttatttta aaaatttgcg
    29041 aggtatgact ttttatactc aacaagaatc aaagagaaac ttagcaatct aacagtagta
    29101 agataaaata ggaatttgat attagggata agattggtaa atagtgtaat atttttacaa
    29161 caataaattt atatagttat ttctggtttc tgaaaagtat tatattttat ttcatattat
    29221 acaaattttt attttataat atcag
//
LOCUS       DAVKZB010000014        27987 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKZB010000014 DAVKZB010000000
VERSION     DAVKZB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.014"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(1049..2275)
                     /locus_tag="VXA80_000550"
     CDS             complement(1049..2275)
                     /locus_tag="VXA80_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV0353092.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2318..3010)
                     /locus_tag="VXA80_000551"
     CDS             complement(2318..3010)
                     /locus_tag="VXA80_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0353093.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(3183..4934)
                     /locus_tag="VXA80_000552"
     CDS             complement(3183..4934)
                     /locus_tag="VXA80_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353094.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVMQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4924..6669)
                     /locus_tag="VXA80_000553"
     CDS             complement(4924..6669)
                     /locus_tag="VXA80_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353095.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6797..7327)
                     /locus_tag="VXA80_000554"
     CDS             complement(6797..7327)
                     /locus_tag="VXA80_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV0353096.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(7324..7779)
                     /locus_tag="VXA80_000555"
                     /pseudo
     CDS             complement(7324..7779)
                     /locus_tag="VXA80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7913..8740
                     /gene="xth"
                     /locus_tag="VXA80_000556"
     CDS             7913..8740
                     /gene="xth"
                     /locus_tag="VXA80_000556"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV0353097.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8752..8850
                     /locus_tag="VXA80_000557"
     CDS             8752..8850
                     /locus_tag="VXA80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0353098.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            8895..9134
                     /locus_tag="VXA80_000558"
     CDS             8895..9134
                     /locus_tag="VXA80_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0353099.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9517..9612
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10012..10593
                     /locus_tag="VXA80_000559"
     CDS             10012..10593
                     /locus_tag="VXA80_000559"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV0353100.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITNVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10593..11855
                     /locus_tag="VXA80_000560"
     CDS             10593..11855
                     /locus_tag="VXA80_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV0353101.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11906..12367)
                     /locus_tag="VXA80_000561"
     CDS             complement(11906..12367)
                     /locus_tag="VXA80_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV0353102.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(12364..13128)
                     /locus_tag="VXA80_000562"
     CDS             complement(12364..13128)
                     /locus_tag="VXA80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV0353103.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(13309..13713)
                     /locus_tag="VXA80_000563"
     CDS             complement(13309..13713)
                     /locus_tag="VXA80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV0353104.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDIANQEGKNVCKATFTMFVTGQRSEDRQVRI"
     gene            complement(13783..15264)
                     /locus_tag="VXA80_000564"
     CDS             complement(13783..15264)
                     /locus_tag="VXA80_000564"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0353105.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(15283..16461)
                     /locus_tag="VXA80_000565"
     CDS             complement(15283..16461)
                     /locus_tag="VXA80_000565"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV0353106.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKREDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            16625..17632
                     /gene="galR"
                     /locus_tag="VXA80_000566"
     CDS             16625..17632
                     /gene="galR"
                     /locus_tag="VXA80_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV0353107.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(17679..18716)
                     /locus_tag="VXA80_000567"
     CDS             complement(17679..18716)
                     /locus_tag="VXA80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0353108.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAAFECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(18717..19070)
                     /locus_tag="VXA80_000568"
     CDS             complement(18717..19070)
                     /locus_tag="VXA80_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0353109.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(19314..20204)
                     /locus_tag="VXA80_000569"
     CDS             complement(19314..20204)
                     /locus_tag="VXA80_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV0353110.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILNLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            20337..20873
                     /locus_tag="VXA80_000570"
     CDS             20337..20873
                     /locus_tag="VXA80_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0353111.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(21039..21845)
                     /locus_tag="VXA80_000571"
     CDS             complement(21039..21845)
                     /locus_tag="VXA80_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV0353112.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(22190..23710)
                     /locus_tag="VXA80_000572"
     CDS             complement(22190..23710)
                     /locus_tag="VXA80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV0353113.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVTV
                     LLGLSYSPALLVQKEKENVIIAGKIGPEPEILANMYKLLIEENTSLTATVKPNFGKTT
                     FLYEALKSGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVSTLEPALRYQAIQSGEIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(23703..24431)
                     /locus_tag="VXA80_000573"
     CDS             complement(23703..24431)
                     /locus_tag="VXA80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353114.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(24446..25210)
                     /locus_tag="VXA80_000574"
     CDS             complement(24446..25210)
                     /locus_tag="VXA80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV0353115.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAVFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(25214..25657)
                     /locus_tag="VXA80_000575"
     CDS             complement(25214..25657)
                     /locus_tag="VXA80_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0353116.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(26339..26635)
                     /locus_tag="VXA80_000576"
     CDS             complement(26339..26635)
                     /locus_tag="VXA80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0353117.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVATLLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(26657..27721)
                     /gene="pepA"
                     /locus_tag="VXA80_000577"
     CDS             complement(26657..27721)
                     /gene="pepA"
                     /locus_tag="VXA80_000577"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV0353118.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         8236 a         6400 c         5202 g         8149 t
ORIGIN      
        1 ggaataacac aagaaaatat taaaatttat actcttcgga aatctcttca aactaggtca
       61 gttccgcctt gccctaagtc taggttactg acttggtcag ttctataata aattgaaact
      121 aaaatagtac acctctactt ctaaaacatt gttagaaatc gatttgactg tcccgatcga
      181 tttgtcctat tcttatttca ttttattata tctacaacct caaagcaggg ttttgagcta
      241 tctatggcca acttcctagt ttgctcttta gccttccatt caaaagtgat gctatctttc
      301 ttagcttgaa tataaaatat cccatttact atatttaaat ttattgatat gttgtaaatt
      361 ataaatgaaa gaacaaatac cttgtcatac aagcattcgc cagggatatt taccttaata
      421 tcgctcatat ggcggattga aactaaaaca ctacaatata tttcttaaat agaccttcta
      481 taaaagctag atttaacctc gataaaaata gatgcagtgc ttctaaaata ctggaatagg
      541 gcatataggt aatcgtagac aagtgtttta tagattgtct cgcaggagat ctaatgatgt
      601 atagcaagca tcactaaaca tatggggtat ttgcattatt ataatatcaa tatgttataa
      661 attctatcat gcaaaagaat gactagaaca tgaaagacag gcacaaaact ttgaacaata
      721 ttctgaaaac aaaaaaagga cgcgagtttc cacaatctca ctcatggaaa ctcacgtctt
      781 tcaaaaaata gggtgaattg aaaactagaa caagacacta gattttctaa atgatgtcta
      841 gtaaggattc taaatgctac accaaattgc tcacatctta tttcaaacca cgggactccc
      901 acaagaaagg aaaatcctat aaaaattctc tctctgagga gaaagaggcc tcaactacag
      961 gtgtaaacta ttatcaattg aaaacaacag tttttacact caattagact ttttagggac
     1021 aggatcactt tcgcttgact gtagtttgtc atttttttga taaagttagc actttttcag
     1081 aaactgtttt gaaagtctca atgatatagt ctacttcttc atcgcttaat ttagtatgaa
     1141 gagggagggt aatttcattc tcaaagaagg cataggcctt aggatagttc gtcatatcaa
     1201 atccaagatt cttataggct gtcaagagag gaagcggttt gtagtgaaca ttacttgcaa
     1261 ttcctgcttt agccaattct tggatgatga ggttgcgttc ttctaggctt gctccttcta
     1321 cacgggtgat gtagaggtgg cgtgaagatt cgacagtttc agtcttgtgt gccaaaggat
     1381 ggatgcgaga acctgcaaaa ccactatcat agcggtccac aatgtcctta cggcgttgca
     1441 acaaacttgg atagcggtcc aattgtacca aaccaagtga agccatgata tcggtcatgt
     1501 tgcacttata ggctggtgta acgatatcgt attcccatga ccccagttgc atcttggcaa
     1561 gagcatcctt agtttgcccg tgaagggaaa ggatttggaa ttccttgtac atctcttcgt
     1621 catcaatcac tggattggct ttccaagtcg cacttccacc ttctgccgtt gtaaagttct
     1681 taacggcatg gaatgagaag gaagtaaaat cagcgataga accagaaggt tgtcctttat
     1741 aagtagatcc caaagcgtgg gcactatcag agacaatgac aatacggtta aaggcctttt
     1801 gccacttgct tgaagcggta aagaagtcac gttttttctc cacgacttgg aacaaacggt
     1861 cataatcgca aacaatccct gcgagctcta ctgggataat caccttagtt ttctcagtga
     1921 tagcttgctc aagcaggtca tagtccatct caaacgtatc tgcttggata tccaccatga
     1981 caggggttgc tcccacgtgc gtaatgacac tacatgaagc cgtataggtc atggctggaa
     2041 ctatgacttc atcaccaggt cccacttcca aaacgcgtaa aatcaactcc agagcggctg
     2101 tcgcagagtt gagacaaaca gtcttaggtg tctgtgtgta aagagacaag cggcgctcca
     2161 gttcttttgt tttaggacct gttgtgatcc aaccagaacg cagggtatcc gctacttcag
     2221 caatttctgc ttctgtgata tcaggcggtg aaaatggaat attgtaattt ggcattgatt
     2281 tgctcctttt atctgtactc attttcttat gactacttta ttttagtacc tcaaacacgg
     2341 tttgaaacat gattttgatg tccccaaaga aactaaactc tcggagatag gcgaggttat
     2401 agcgcatctt ttcaggaaga acatgctcca cataggcctg atcaactgac agacctttct
     2461 ccgtcatttg actgatgatg gtgtcctcat ccttgtagtt gatgctggct ggagaggtaa
     2521 ttcctgcttg caagagcaag gttgccatca tttcagggct atactgctct gtataacgtg
     2581 gcacttcagg tcgtgtaccg acaaaggaca tctcaccttt aaggacattg accaactgag
     2641 gcagttcgtc caaacggaca cgtcggatga aatttccaac cttggtaata cggctatcgt
     2701 tagcagaagt caccagactt ccttttttat ccgcatccgt caccatggta cggaacttcc
     2761 aaatcttgaa ccgacggttg tactgggtca cacgctcttg cttgtaaatc actggcccct
     2821 tgctatccaa cttgatccaa atgctcaaga tgagaaagat gggagaagtt agaaccagta
     2881 agaccaaggc caaaacccag tccaagcaac gcttgaaaat cagcgaaccc ttccttttag
     2941 agacaagctg gtagtaagac tcaacctcgc ttgatttcat ttccacaggc aagtcttccc
     3001 atttcagcat gctgttctcc tttgttttta ttatactatt tgtcaacatt tttcattata
     3061 ccacaaaatg gaaaaggcgg tgaaagaaat ctgtaatttg aaggatttcc ttctagtact
     3121 caatgaaaat caaagtacag ctttgagatt gcagataaag ctgacatggt ttaaagagat
     3181 tttcaaagag tataggccat ggcccctgcc tgcaaactat acatcttgtg ataggttcct
     3241 cccagagtca agagttcctc atgggttcca ctctcgataa tgcgtccctt atccaagaca
     3301 tagatgcagt tggcgtcttg aatagtagaa aggcggtgag cgatagcaat agttgttcgg
     3361 ccctgtctca tcttcgccag agaagcttga accaaacttt ctgtttcaga gtcaatattg
     3421 gctgtcgctt catccaaaat caggatttta ggctggctgg cgactgttct agcaaaggca
     3481 agaagctggc gctgcccagt agagaagctc gaaccacgct cggaaacagg ggagtcgtac
     3541 ccctgaggaa gttcttgaat aaaggaatct gcatccacaa aggctgccgc agcctgaacc
     3601 tgctcatcac tgatttcttg gtacatggcg atattggact taatagttcc atgatagagg
     3661 aagggttcct gtaaaaccaa accgatgttt tttctcagct cttcttgact gaaatccctg
     3721 atatccacat catccaagag aactctccct gactggaatt cataaaagcg catgaggaca
     3781 ttgataatag acgatttccc tgaacctgta tgacctacaa aggcaatggt ttcaccctta
     3841 ttaacagaga aagaaatgtc atccagaatc ggatgtttac cgtcatacga gaaacacaca
     3901 tgttcaaaac ggatattgcc ttctttgact ttggcttgcc cattttcttg aaggggctca
     3961 taggtcctct catcgattaa ggcaaagaca cggcctgcag aaaccataga cgtttgcagt
     4021 gttgaaaagt tttgcatcac ctcaatcaaa gggtcaaaga ggcggttgat gtactggata
     4081 aaagcataca tggtcccggc cgttatcccg atagaaagac cacgatagcc aaaataggtc
     4141 atcaagacag cataacctag aagtttcaac aaactcatgg caggtctcaa aaagagggca
     4201 tccaaggcta cagaacggtt ggcataggcc aagtgctctt ggttgatttc atcaaattct
     4261 gcctgcaggc gcttctcttg attaaaagcc tgaataatcc tgattccctc gatattctct
     4321 gccagcttac tattgatatc tgacaagaga cttctggttt tctcgatgat tttcactgac
     4381 ttttttcgat agagattgac caaaaggaaa atcaagggga gaaagagcaa gactaatgct
     4441 gtcaaacgaa aatccagcac caacatggta taaagggttg tcagaaagat aaaaactgct
     4501 gagataaagc tggataaaat cccagaaaac atatcactaa tcgtctcggt atcgttggtc
     4561 aaacgagaaa cgatagaacc tgctggcgtc ttgtcaaagt aagacatgcc cagtttctcc
     4621 atattggcaa aggcatcccg acgaatatcc ctaacaatac tgtaagacac gcgcgcaaag
     4681 agaagattgc cgacatactg aactacagtt tgtaggatgt agagaccata gtagaccagc
     4741 aaaacggtca cggctagttg gttaagattg ctgagatact ggtcgataaa gtgggaagcc
     4801 acaaggggaa tgacactttt aatgaccgtc gtcgctagga gaaaactgag tgccaaaaag
     4861 gtaaggagtc cataaggctt gagataggac atcaagcgct tcaatacagt ccattgttct
     4921 tgtttattct gcatcttctt ctcccttcat ttccagctgc tgagactggt aggtttgggc
     4981 ataccagcca tccaaagcta gcaagtcttc gtgcctgcct cgttcgataa tttgaccatt
     5041 ttgcaggact aaaatcaaat ctgcatggac aactgcactc aggcgatggg cagtgataat
     5101 ggttgtcttg tcctttcgcg tctccttgag gttgtcgata atcgcatact ctgtcttggc
     5161 atctacggcg gataaggaat catccaaaat caagatatca gggtctaaaa tcatagcccg
     5221 actcatagcc aaccgttgct tttgaccacc agaaagactg actccttttt caccaatcag
     5281 cgtatcaaat ccttgaggca tgtctacaat atcttggtaa actcgggcta gcttagtagc
     5341 ttcctcgacc gctgaaaggg gcaagttagg attgccaaag cggatattgt ctaggattga
     5401 agtcgcaaaa agaaactggt cctgaggaac atagcccatg agactgcgaa ggtctgtcag
     5461 acgatagtcc cgaatatcgt gaccgtttag ataaatggcg cccttatcca catcgtattc
     5521 acgcaagagg agcttgatta aggacgtttt cccagagcct gtctgcccaa ccaagcccag
     5581 tgtttgccct tttgccaaac taaagtgaat atccgtcagt gtttcctcat tttcaaaagc
     5641 aaagctgtca atggcatact ccaaacgccc attttcaata ccatccagag gaaactccgg
     5701 gtcttgtaca ggagattcct gagacaaaag attttcaatc cgctggtaag aaaccttccc
     5761 tcgctgagta gtattaaaga ggaagccgat ggccataaga ggccagacca gcatatccaa
     5821 atagctgata aaggtgacta gattcccaac tgtaatctgc ccttcctgaa ccaccaagga
     5881 gccaaccaaa agcgttaaaa cataggacga accaacaaac aagagaacca tagggtcaaa
     5941 gagactatca tatttcatgg tttgcaggtt cttttggaag gttaattcat tgactgcctg
     6001 aaaagacttc aactcgtctg cctgataacc gaaagacttg gtcactttga tacctgatac
     6061 ggactcctgt accttgttat tgagttcaga aaaagcagct tgggattcgc caaaggcctt
     6121 atgagtcttt ctccctaggc gactagtcgc ataggccatg aaaggtaggg ggagaatggc
     6181 aacaagagtc atctgccatg agatgctaaa gagcatggtc aacaaagtca ccagagccgt
     6241 gatagaggca tccaccgcag acatgacacc gccacctgct aaacgagtca aggcattgat
     6301 atcattggtt gcgtgtgcca tcagatcacc cgtccgatag gtttgataaa aggctgacga
     6361 catttttgtg aaatgcttaa acaagcgaga ccgcatgatc tgtcccaagc aataagaggt
     6421 cccaaggata tacatacgcc acacatagcg caaatagtac ataccaaagg ctgcaagtag
     6481 caagtaaaat aggctaagaa ggaggtcctg ctgggttaat tgccccgatg tgatggcatc
     6541 aataacccgc cccataacca taggaggaat gagattgagg acggaaacca agaccagggc
     6601 cacaattccg actagataac ggcgtttttc taacttgaaa aaccaccaaa gtttttgaat
     6661 aatggacata aaatcccttt ctgattgcaa atggaaacct gaggccaata ctcaatgaaa
     6721 atcaaagagc aaactaggaa actagccgca ggctgctcaa agcactgctt tgaggttgta
     6781 gatagaactg acgaagtcag taacctacat acggcaaggc gacgttgacg cggtttgaag
     6841 agattttcga agagtacaag accccaggtt tttctttttc ataagttacg actgtaacag
     6901 cacccttgtc atattcagca ataaagatat tggctacatt gtcatgccct tgtttactga
     6961 ggttatcaag caaccactct tcgctacgac caatcgattc caagacatct acttgaatca
     7021 caccgtcagt cacaactgga tacttaggat tttcatctcc catttgcaca acgataagtt
     7081 gcccattttg ctcttgcaca gctcgtttga cttgcttcat ctggaaaatc ccttggctac
     7141 gaagtttgag ggctacatcc gatgcagaca aaccaactga acgacaggct tctgggtcaa
     7201 tattcccatt tttgataagg agagttggct taccatcaat caaacgtttc acaaagtgaa
     7261 cattgttgtt gagccatttg agagtcaaga ccagtttgat taaaatttcg atataattaa
     7321 gtgtcatttg ttcacatcca ccagttcaat tgcgtctgtc ttatacaatt cgactttctc
     7381 taacaggtac ttgtctggct cacttccgtt tagggcacga taatagcgat ctcccacctt
     7441 gataagtgcg ccatctgtgt ttgtggaagt attaacatag acttctgact tgtctactcc
     7501 caaatctttg gaaacaacct cgatgaaatg aagtgaagtt tgaaatttat tgttagaaac
     7561 ttgattgttt tgataagtcg tgatgctgac caaaagcaga gctaaataag gccagagctg
     7621 aaatcatgac caactcacga aattcggttc cctttttatc atggtatgcc ttgaaagcaa
     7681 aaaaactgtc acagctaata gaacaatccc aaagccaacc atgataccat tttgctgacc
     7741 gatttggctg agtacatagt cataagagta gaatttcata tattttcact ccctttcata
     7801 aaatcattct taattataaa cgaaagagag taatttttca aaccatacgt tggggaaata
     7861 gatctttttt tggtataata aaatctataa tctgaatgaa aaaggtaact ttatgaaact
     7921 tatctcatgg aatattgatt ccctaaacgc tgccctaact agtgactcag ctcgtgccaa
     7981 attgtcccaa gaagtcctac aaaccttggt cgctgaaaat gctgatatca ttgctatcca
     8041 agaaaccaag ctttctgcca aaggacctac aaagaaacac gtggaaattt tagaagaact
     8101 cttcccaggc tacgaaaaca catggcgctc ttcccaagag cctgcccgta aaggctatgc
     8161 tggaaccatg ttcctttata agaaagaact tacgcctacg atcagttttc cagaaatcgg
     8221 tgccccttct accatggact tggaaggccg tatcatcact ctagaatttg atgcattttt
     8281 cgtaacccaa gtttacactc caaacgctgg tgacggtctc aaacgcttgg aagaacgcca
     8341 agtctgggat gttaaatatg ctgagtattt ggctgaacta gacaaagaaa aaccagtcct
     8401 tgcgaccggt gactacaacg tagcccacaa tgaaatcgac cttgcaaatc ctgctagcaa
     8461 ccgccgttca cctggattta ctgacgagga acgtgctgga tttaccaacc ttttggcaac
     8521 tggatttaca gacaccttcc gccatgttca tggcgatgtt cctgaacgct acacttggtg
     8581 ggcacaacgc agcaaaactt ctaaaatcaa caatacaggc tggagaatcg actactggct
     8641 cacaagtaac cgtatcgctg acaaggtcac taaatctgat atgattgact caggtgctcg
     8701 ccaagaccat acaccgattg tcttggaaat tgacttgtaa ggattttcta tatggactac
     8761 aatgcggtca ttcccgagtt tcttgtatcg aatatcgaac agtcccggtc cttctactgt
     8821 ggtttactgg gttttaggat tgaatactaa cgtccagaag aaaactttct ctttctcttg
     8881 aagagtgtca actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctacc
     8941 cctttggtcg tggagtcaat ctctcctttg gcataaagga tgttccaaaa ctctatcaaa
     9001 aagtgatgga ggcaaactat cctatttatc gtctcttgac caaaagaaag tttcgtgtca
     9061 gtgatcccta tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa
     9121 gatttagcga gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat
     9181 tgtcaggtat ctaaaaactt gatagtatgc cttttatgat ggaaaaatct tctaatactc
     9241 ttcgaaaatc tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactgactc
     9301 tgtcaattct atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt
     9361 gctctttgat tttcatttag tataaatgtt agtttacaat ggagtttttg ttcaccctct
     9421 cttctgtttg ttcgtttttt aaaccaaaac actaacaaaa tagttgattt ctaaagttat
     9481 ttagtgtaaa ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt
     9541 ttatgtcgtg aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag
     9601 tcagcagtga gatatggccg tttttgccat gtctatttag gggacttact tagagattaa
     9661 gtaggtcttt tttgaatatt tttaatattt cccttaatta gtgcggacgg gaggtgaaat
     9721 tatcctgtga ataatttcag aaatccaggg aatttcatcc ctaatttttg agcccctagc
     9781 tctttcattt ctaggcttag gctaaaatag ttccccgaac tattttactc tcaaaaatcc
     9841 cgtcatagac aataacaaga ttattgtcta tgataccact acaacgggaa atatttgata
     9901 agactcgctt cgctcgtaat agttttatca aatatatagt attagcaaaa gggatcacta
     9961 tagaaacatt taaacaacaa gtaaattaac ttttagactt taggaggtct tatgaaatta
    10021 ttagaagagc gcatcctcaa ggatgggcat atcttgggtg ataacatcct caaggtagat
    10081 tcctttttaa cccaccaagt tgactttagc ttgatgcgag agattggtaa ggtttttgcg
    10141 gaaaaatttg ctgctactgg cattaccaat gtcgtaacca ttgaagcgtc gggtattgcc
    10201 ccagccgttt ttacagctga agccttaaac gttcccatga ttttcgccaa aaaagctaag
    10261 aacatcacca tgaacgaagg catcttaact gctcaagtct actcctttac caagcaggtg
    10321 accagcactg tttctatcgc tggaaaattc ctctcaccag aggacaaggt tttgattatc
    10381 gacgatttcc ttgctaatgg ccaagctgct aaaggcttga ttcaaatcat cgaacaggcc
    10441 ggtgccacag tccaagctat cggtatcgtg attgagaaat ccttccaaga tggtcgtgat
    10501 ttgcttgaaa aagcaggcta ccctgtccta tcacttgctc gcttggatcg ttttgaaaat
    10561 ggtcaggtcg tatttaagga ggcagatctc taatgcaaac tcaagaaaaa cactcgcaag
    10621 cagccgttct gggcttgcag cacttactag ccatgtactc aggatctatc ctggttccca
    10681 tcatgattgc gacagccctt ggctattcag ctgagcagtt gacctacctt atttctacag
    10741 atatcttcat gtgtggggtg gcaaccttcc tccaactcca actcaacaaa tactttggga
    10801 ttggactccc agtcgttctt ggagttgcat tccagtcggt cgctcccttg attatgattg
    10861 ggcaaagcca tggtagtggc gctatgtttg gtgcccttat cgcatctggg atttacgtgg
    10921 ttcttgtttc aggcatcttc tcaaaagtag ccaatctttt cccatctatc gtaacaggat
    10981 ctgttattac cacgattggt ttaaccttga tccctgtcgc tattggaaat atgggaaata
    11041 acgttccaga gccaactggt caaagtctct tgctttcagc tattactgtt ctgattatcc
    11101 tcttgatcaa catctttacc aaaggattta tcaagtctat ctctattttg attggtctgg
    11161 ttgttggaac tgccattgct gctactatgg gcttggtgga cttctctcct gttgcggtag
    11221 ctccacttgt ccatgtccca attccactct actttgggat gccaaccttt gaaatctcat
    11281 ctattgtcat gatgtgtatc atcgcaacgg tgtctatggt tgagtcaact ggtgtttatc
    11341 tagccttgtc tgatatcaca aaagatccaa tcgacagcac gcgccttcgc aacggttacc
    11401 gcgcagaagg tttggccgta cttctcggag gaatctttaa caccttccct tacaccggat
    11461 tttcacaaaa cgttggtttg gttaaattgt caggcatcaa aaaacgcctg ccaatctact
    11521 acgcagctgg tttcctggtt ctccttggac tgcttcctaa gtttggtgcc cttgcccaaa
    11581 tcattccaag ctccgtcctc ggcggtgcca tgctggtgat gtttggtttt gtatctattc
    11641 aagggatgca aatcctcgcc cgagttgact ttgctaacaa tgaacacaac ttccttatcg
    11701 cagctgtttc aatcgctgca ggtgtcggtc tcaacaacag taatctcttt gtcagcatgc
    11761 cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt agccagccta ctcgctattg
    11821 tcctcaatgc cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt
    11881 tgtgtgacaa aataaaaaca tctacctatt catatatgct agtgtcatag tcctgaaaat
    11941 catctgcatc aagaacaatt tcacaactct tatacttttc tcttacaagt cgttcggctt
    12001 catctggatt ttcagcctct atacttacta aacgtgataa agtttctgta atttctactg
    12061 tatacttttt cagttttaga ttgctttcca ataattcacc tattaacaat aatctatgat
    12121 aggagctatg tagccactct ggcaatttat ctcctacttc ccacccatat ttttccttca
    12181 tcaaagctag ttgtttttcg tcaccaaatt tcatattttc agataaccat ctcacacaat
    12241 cgggatctga taaggtacag acgattaaac ttaggttatc gtaacggctt tcagtttcat
    12301 aatcaggcca gatgactttt aagaaatcat acacctcttc tgatgcaaca agttgtttcg
    12361 ttttcataat ttccgatact ttcctctacc tttaaattct attatttctt tgtctcttaa
    12421 tatttgaagc tgttgcctaa tcttttcttt gatatgattg ttcttaacaa agatattttt
    12481 taggtcactt tcaaaacgat acatatcttc aagggtaaat tctgaaccct ctatcttatc
    12541 tatacaattt agaatttcta ttgtccaacc tcttgatgac agagagctct tccttaaaaa
    12601 taaaccttgc ttaaattctt ttgtaacttt ttctggatct ctaacttgtc catcttgcac
    12661 aagaaatatc cttcctttag aaggtacttg tgataaatca atgttacaac caatccaacc
    12721 tgctcgtcta gcagttggtg caagtggttt tctttgaata atcgatttcg gtgtaacaaa
    12781 ttgcttcgga aggacaagaa agttatttac ctcaaaattt tttgtgtaag ttaaaaaaaa
    12841 gaaattagga ttattatctg cctgcacacg cttcatcatc gttgcataag caccatcatt
    12901 gattgttgat gaaaaatttc cttttttgct ctttagttca aactcctcac tacaatgatt
    12961 acagtaaaaa tctgctacag gccgattatt ttcaaaatga tttaagggat tgttcccaca
    13021 attagggcaa taagactgac gataaaccca atcctccgtt aagatacgtg ccttttggct
    13081 attactttta taggtttcta ctaattctaa attaaagtgt aattccattt aaaaattcct
    13141 cactaatatg agaactcact cgatataagt aattaaaatc tactataact agtataccac
    13201 atatatgtac ctattacatt tgtatttcat cactctcttt tatatttcct tctaacatat
    13261 ctcataaatt aaaaatgagt gagataaatt tatcccactc acctttcttt atatccttac
    13321 ctgtctgtct tctgaccgct ggcctgtgac aaacatggta aaggttgctt tacagacatt
    13381 tttgccctct tgattggcga tatccacatc catcacacag gttgtacggc cttgatggac
    13441 acattctcct ttaatggtca atacatcgtc gagttttcct gccttaaggt agttgataga
    13501 ggattggagt gtcaccccat caagtcccag cgagataacc accaaaccac tgatttggtc
    13561 acatagcgtg aatagatagc caccatgggc attgccatag taattgagcg acgagttcac
    13621 tactttggtc gtcaccacaa cgtgaccatc tctcattttt tcaatttcgt aattttcaaa
    13681 ggcagatata gcgtcaaaat gaaaatcttt catcgtttcc tcctgatatt tcaaacgaca
    13741 ctatgatact actttttata ggactgtgca aggagaaagc tcctagtcta gtgaaattcc
    13801 gacatgttcc acaaagcgca taaaggctgt ctgcccttgt tctgtctgct tgtagactcc
    13861 tgcatcctca agtacacgca caaagatagc acccacagag tccttgacga ttgcaagggc
    13921 tttttcttta tccgttaggt ctggatgttg ggatttgagt tggtctgccc actcctgatg
    13981 ataatcggca actgtaacag cttctcctac aagatagcta gcgacttgct ccacttcttc
    14041 tttcagacgt ggtggcaaga ttgccaagcc catgacctca atcaagccga tattttcctt
    14101 cttgatatgt tggacatcct tgtggggatg atagatgcca tcaggatgct ctgctgaagt
    14161 ctgattgtct cgcaagacca agtccaactc aaactgtcca tcacgcttac gcgctattgg
    14221 tgtgatggtg tgatgcggtg tcccgtctgt ctctgccaaa atctgcacac tagaatctga
    14281 atactgacgc cattcctgga aaatcttatc agctaagtta atcaaatcct ctttggaatc
    14341 cgaagtcaag cgtaggacag acattggcca cttgataatt ccagccttga cctgctcaaa
    14401 accagcaaat cggaaggtct tttgcaaggg agccaattcc ataggaaata cgtgacggcc
    14461 tccctgataa tgatcatgag ttagaataga gccccccaca atcggcaggt cggcattaga
    14521 tccagcaaaa tatcccggaa actgctctac gatagccaac agacgttcaa aactctgacg
    14581 actaatggcc atgggacgat gctggccatc taaaaagata caatgctcat taaagtaagc
    14641 atagggcgaa tactggaaac cccattcctg accaaccatt tcaaaacgga taatccggtg
    14701 gttggtgcga gctgggtggt tgacccgacc atggtaaccc tcattctcta aacaaagttg
    14761 acactgagga taattactag cttgcaccaa cttggctgcc gcaatctctt tcggatcctt
    14821 ttcaggctta gagagattga tggtaatttc aagttctcca taatcagatg gaacacgata
    14881 agcgatattt ttagcaatgg ccttgagttt gatgtagtca tttttctgac tgagttgata
    14941 aaaatcctct atcgcttgct caggagagta ggcataggct tcccagaagt cacgattgac
    15001 ctgactcgga caaggagtca ccaagtccat caattcagta ccaaggattt cacgcgcagt
    15061 ctgactatcc tcaatcgtct ctaatcgaac ggcttcctca actagctggt ccttgaggtc
    15121 aatcaattta tccagattgg tctcaacttc caaaacacct tctcccactc gtgccaaaac
    15181 acgattggtc aggtagattc gatccatttc ctcaaatgaa ctttcagaaa tgacatgtgt
    15241 tacaaattta tctactaagg tcactataga gcctcctttt gactagtcaa ggacgcgagt
    15301 gccacctgca acttcagcga tatagaagct tggagcgtat ccaactactt cctcgtagtg
    15361 tttgcctaca gcttccttaa aggcctcaac agtatctttt tgaaccaagg caatggcaca
    15421 gccaccaaaa ccagcccctg tcatacgagc accgagaact ccttcttgtg cccaagctgt
    15481 gtgaacaagg gtatctaatt ccaaaccagt tacttcataa tcatgctcca gagaaacgtg
    15541 tgacgcattc atcaaacgac caaatgtttc caaatcacct gcttgaaggg ctgcttgagc
    15601 tttaagggta cgttggtttt caagcacagc atggcgagca cgtttcaaac gattttcatc
    15661 tttaatcaaa tagctatatt ggtcaacggc ccactcgtcc aattcaccca gggtctgaat
    15721 atccaaggca acttgcaatt cttccactgc tttttcacac tcagcacgac gttcattgta
    15781 tttagagtcc gccaattcac ggcgtttgtt ggtgttcatg ataacaacga cattgtcctt
    15841 caaatcaagt ggcaccaaat catactctaa ggtgttggta tcaaggtaaa tagcacgttg
    15901 atcagccccc ataccaatag caaactggtc catgatacca gagttgactc cgataaagtt
    15961 gttttctgtt tgttttccga ttttaaccaa atcgagacgc tctaatttta aatcaaagag
    16021 atgctctgcc acgactcctg tcaagagctc caaggatgct gaagaagaca aaccagcacc
    16081 atttggtata ttcccataaa cataaaaatc aaaacctttg tcaatcacgt gcccagcttc
    16141 ttgcaagaaa tgaaggacac cttttggata attggtccag ttgtgctctt tttcaaactt
    16201 gaggtcagcg agaggcactt cgataatgcc cttgtcctca aagttagctg agtagaaacg
    16261 taaaacttgg tcttcacgct tgcgagctgc accataagtt cccaaggaaa tagcagctgg
    16321 aaaaacgtgc ccaccgttgt agtctgtgtg ttcaccaatc aaattgatac gacctggtga
    16381 aaagaaggtt tggtctgctt cttgaccaaa aacagcaaga aagtctttgc gaagagtttc
    16441 agcagtaaga tgttgtgtca tatgatttct cctttgactg tccgttaagc caccttaacg
    16501 tgtaatcgtt ttcttctatt gtatccaagg gatttcccaa ggtcaatcct ttttactaaa
    16561 attttactaa aatatcagta aaaagcagaa aaatatgata tactaaccta atcaaggagg
    16621 atttatggct accttaaaag acattgcaca gctagcctct gtctctatcg cgaccgtatc
    16681 ccgtgttctc aaccgcgacc agagcctatc tgttacagaa gaaaccagac accgtatttt
    16741 aaccgttgct gaagagctgg gctacaccaa gcacctcaag acaggcgagt cccataaacc
    16801 caagcaaaag attgccatta ttcaatgggt cagcgaacaa ggggagctag acgacctcta
    16861 ctactaccag attcgcctag gaatagaaaa aagagcccaa gagttggact acgatatctt
    16921 gcgctatttt aatgaccacc cttttaccct aagcgaggaa gtgattggga ttctctgcat
    16981 cggaaagttt agtcgagctc agatttctgc ctttgaagaa taccaaaagc ctcttgtatt
    17041 tctagacagc gatacacttt ccctgggaca tacctgtatt atcacggatt tttacactgc
    17101 tatgaaacag gttgtcgatt atttcctcag ccaaggaatg gaccgtatcg ggattctaac
    17161 aggccttgaa gaaacaacag accaagaaga aatcattgag gataagcggc tggaaaattt
    17221 cagaaactac agtcaagcga ggggaatcta tcatgatgaa ctggtctttc aaggaagatt
    17281 tactgcccag tctggctatg acttaatgaa ggaggccatt cagagcttgg gagaccaact
    17341 tccgccagca tttttcgcag ccagcgatag tttagctatc ggtgccctcc gtgccctcca
    17401 agaagctgga atcaacctgc cagatcgcgt cagcctcatt tcctttaacg acactagtct
    17461 gaccaaacag gtctatcctc ccctctctag tattacagtt tatactgaag aaatgggccg
    17521 agcaggtatg gatattctta acaaggaagt cctccacggt cggaaaatcc ctagcctgac
    17581 catgctggga accagactga cattaagaga aagtacccta aatcaagaat aggataacat
    17641 aaaaaacgaa taggagtttt agaactccta ttcgtttttt attcgattac aatcatagac
    17701 ttaatggtct tacgttcatc catatctttg taggcttggt cgatatcttc cagtttataa
    17761 cttgaagtaa agacgcgacc tggattgata tcaccatcaa ggacggcttt tagtaaaaat
    17821 tgcttatcgt atgttgtagc agaagctgcc ccacctgcta cagagatatt ttgcataaat
    17881 gtcgaaccaa gagcacgatt attatagtgt gggactccta caaagcccat acgccctcca
    17941 ttatgaagaa cacctagcgc ctgttctata gcagcctccg taccaacaca ttcaaatgct
    18001 gcatctgctc ctccaccgag gatttcacgc accttggtaa ttccttcttg accacgttct
    18061 gcaacaacag ctgtcgcacc tgactccata gccatctttt gacggtcttc atgacggctc
    18121 ataaggataa tttgtgatgc tccacgcatc ttagccgcga tgacagcaca ttgaccaaca
    18181 gccccatcac cgataacaac aaccttgtcc cctttttgaa catttgcaac acgcgccgca
    18241 tgatagcctg tcggcatgac atctgcaaga gtcaaaaggg acttgagcat cccttctgta
    18301 tagtcagaag gttgaccagg gattttaacc agcgcccagt ttgcatagtg gaagcgaata
    18361 tattctgcct gaaaatcacc ccccaaatta ttgccaatat gattgtcgca agaaccgtca
    18421 aatccagcaa gacaggcatc acactcacca catccatgtg taaaagggac aatcacaaaa
    18481 tcacctggtt tcaccgtcgt aatggcttcc ccagcttctt caacaatccc aatcgcttcg
    18541 tgtccactat ttttgtgtcc agctttcgtt tctggattac ggtacctcca taaatctgaa
    18601 ccacaaacgc acgcacgaac cacacgaata atcacatcat ccgcttctat tatttgcgga
    18661 cgttcaatgc tagcaagtcc aacctgacct gcctttgtat atactgctga tttcatttaa
    18721 aattttcctt ccttataaag tttaattttg agatttaaac gatttaaagc tgtctgtagc
    18781 tgagacaagc gctcctctaa tttttgcttt tcctcttcta aaataccaag cctctcctct
    18841 ctcgtttcat ctcccttttg gtagagcgac atatagtcaa ctaaactatc tacagagaca
    18901 cccgccgaac gaaaacactt aataaattcc agcgcttcga tatcctgatc ttgaaaatca
    18961 cgaatcccag tagcagtacg agtaatcggt ggcacaagac caacccgttc ataataccga
    19021 atcgtatccg ctgaaattcc caacaaatca ctggcagatt taatattcac acgctaacct
    19081 cctctcattt cattcactga gttcacgcta ttctaactca cttatcttat tatataactt
    19141 tgagttgact ccaagtcaag cattttgtat ctaatcgaaa aatttttatc attaacaatc
    19201 atatatctgg agtttataca atgctcttct cttaccaatt tttgaaatat ttaaataata
    19261 aagaaattac tctattccaa ataagatagt acccaagtat ttttcacttt tttctaatgt
    19321 tgatgctcat aactccgttc caagtcacac acctttcgct tatgagtttg atgagtttct
    19381 aggtcagcat caatttcaat ggtaatattt tgaaaaccac aatcttttag gaaaattcga
    19441 atagactctt tacaagtttc catatgttcc atttctttta gacaaacatg gacaatggca
    19501 tttttttcca aagcatccat agtccagaga ttaagctgat taaggctggc cacattatcc
    19561 aatcgttcca ggccactctt tacttgcttg atatcaagac cttctggcac agcatccaag
    19621 aaaatcttga gtgtagacca aaaacgtgaa agggcttttg aaagaataaa gaaagaaatg
    19681 acaagggaca aaagaggatc taggatatac cagtccgtaa atcgaagaac aatcgccatc
    19741 aggataacag ctacccaccc tagcgtatct tccagaaaat gcagattcag aatagactca
    19801 ttctttgtct ttcccttacc aaccaccaga ctcgctaaca gattgatagt aatcgcaata
    19861 attcctaacc agagaatccc ctcatcattg actggttgcg gatgcaaaat cttcgtgaca
    19921 ttttccaaaa tgactagaac agagcccgtt acgagaatca cagctgttac caaggctcct
    19981 agcaggctaa accgcttata gcccaaggtg tactgattgt cttcttcacg attggagatt
    20041 gtttctagaa aagctgatat tccaattgca atcgcatctc ccaagtcatg cacagagtca
    20101 gcaagaacag cgctagaacc aaatactcca cctgcaataa actcaacaat ggcataagtc
    20161 aaatttaaga aaaaagccac ccaaacagca tattttgcct tcatatttct cattcctttg
    20221 ttataataga tttatgaaca ccttgttcat tatcattatc cactaaaact caaagaaagg
    20281 atagaagcaa aacgtcagct ttattcagtt ctgaacaatt tgcctcaagt gtccatatga
    20341 ctaacattga ccgccgtatc agcaaaacca aaaaagccat ctatcaagct tttatacaac
    20401 ttttgaatgc taagggctac gaggccacta ctgttcagga tatcattgat ctcgcagatg
    20461 tgggacgatc caccttttac tgtcactatg agagtaagga gcttcttctg gaccagctct
    20521 gccgctacct cttccatcat ctttttgaaa gagagcaagc catttcaacc gaggactacc
    20581 tcgcccacct ctttctgcat tttcataaaa accaagacca tatcaccagt ctgctatttt
    20641 ccaaaaatga ctacttcctc cgtcaactcc ataaagagct agaacaccat gtctattccg
    20701 tgctagctga taatttaaaa gaagcccacc ctaatctgcc tacttcttac ctccaacact
    20761 tggtcatgtc caactttatc gagacattga cctggtggct caaaaaaggt caagatttca
    20821 cagaccagga agttgtccaa ttttatctag accttctcat tcctaaaaat tgaatataga
    20881 gtaaagctca gttgtcttat ttctagatta ctgagttttt tatcttttca acaacaaaag
    20941 aggacccgcc gatcctcttt ttcatactat aaatccttga ttatcaacta tatctgtttt
    21001 aatcgaaatc tcaaaacagc actttcaaac atcttttcct agttaagtaa atcagtattc
    21061 tgcttagctg ccttgctcca ttgataccaa ccaactagac tgttaatgag ataaattaga
    21121 tatttccctt gaatttgcag gctttctccc caccagagat agattgaaaa gacattggta
    21181 gccgcccaga atatccactg ttcacggtaa acagctgtca tgaggatttg ccctacccca
    21241 ttggttgcat ctgtgattga atcacgatag ggacgattgg caccaataga ctgataaatg
    21301 aagccaaagg ccaaccacca aagcacacta atggaaagat actttgtcca gcccttgccg
    21361 tccagtttac gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg ataaatccaa
    21421 actagaagtc caattggctg catgactgtg aagtaaagtg tcgtcagcac ctcaccataa
    21481 aagcctttct gtagggccaa aataaggtaa ataacagagt taatcaagcc aaaaagataa
    21541 ttacttgctc gaccttccga tacaaagatt acacagataa tccctgtcaa gctacaaatc
    21601 atcccaatcc agtcaacaat acgatgttcg taaaccaact ccagccagag aggaaaactt
    21661 cctaaaacca gcaaataaat ccactgggca aaactacgat gggcaaagag gtcatcccag
    21721 atagccttca tagttcctga aaatcctaaa tcagccatag ccgcaaccat acgacggtaa
    21781 ccacctgaca tttcacctag ggttgttttg atattttcaa ttttcttttg caaataagta
    21841 tgcatcattt ctccttttgt ttttaaagag ccgtgtctgg atagactttc ggacgcaacg
    21901 ctctattaga taatgaactg cctatacaca agatttctaa ccttagtcga catgagctga
    21961 aacctcttat ttgttaagta gttcacaaaa tattatacac ctattttatg aatagtcaac
    22021 tgtctttaca gtaaaatttt agaaaatcat gaaaattttc tctttctttc cattttaagt
    22081 gacattcagt cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc attcttttat
    22141 ctagtcgttt aaggaagttg agttcagtat gtttaaagtc tctgtcccat catttcttca
    22201 acaaaccttg ttcttggaga aactccttgg ctacttgctt tgctgacttg ccttcaacac
    22261 cgacttggta gttgagctgg ctcatctggc tttctgtaat cttaccagcc aatgtattaa
    22321 gaactctttc caactctggg tgtttcttga gaagagcttc tttcatgagt ggagcccctt
    22381 gataaggtgg gaagagttgc ttgtcatctt ccaagacctg taaatcataa cgctccaatt
    22441 ccgcatcagt cgaataggca tctgtaattt gaatttcacc tgactgaatc gcttgatagc
    22501 gaagagcagg ttccaaggtt gaaacattga gattgagacc atacattgat tgcaagccct
    22561 tatttccatc ttcacggtcg ttaaactcga gtgtaaagcc tgccttcaac tgaccttcca
    22621 cttttttcaa atccgaaatg gtcttcaagc catattcttg cgcaatcttt ttcggaacag
    22681 ctacagcata agtattttga taagacatgg gtttaagata ggctagatga tcctgtttgg
    22741 caatgccatc acgcgccacc tgataaacct gctctggctc atgactcact ttaggtgatg
    22801 gttgaagcaa actttcagtc accgtaccag taaattcagg atagatatca atgtctccgc
    22861 ttttcaacgc ttcataaagg aaagttgttt tcccaaagtt aggcttaacg gttgctgtta
    22921 gacttgtatt ttcttctatt aaaagtttat acatattagc caaaatttct ggttctggac
    22981 caattttccc tgcaataatc acgttttctt tctctttttg aaccaagagg gctggactat
    23041 aagatagacc cagcaatacc gtcaccaagg caaaacctga gaaaatcgtc cgtaattttg
    23101 ctttttccat tacttttagt aggaagttaa aggcaatagc aagcactgca gaagaaagtg
    23161 ccccaatcaa aatcaaactg gcattattac ggtcaattcc caacaggata aaggaaccca
    23221 gtccccctgc tccaatcaag gccgccaagg ttgccgtacc gataatcaaa acagctgccg
    23281 tccgaatccc agacatgata acaggcatgg caagtggaat ttcaaatttc ttgagacgtt
    23341 cccatctggt catcccaaag gcaatcccag cctcttgcag gttcggatca attcccttca
    23401 gcccagtgat agtattttgc aaaataggga aaatcgcata aatcactaga gctgtcaaag
    23461 ccggcaaggt cccaattccc atcaaaggga taaagagccc caacaaggcc agagacggga
    23521 tggtctggaa aatacctgca atctgcaaga cccagtcggc cagcttctca tgatagcgaa
    23581 gaaaaacagc caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa agtgacaact
    23641 gcaaatgttg agatagagct gtcaaccaat cactaaaacg atcctgaaaa gttgcaatta
    23701 aattagtcat gaacactacc tccaaacaag tctgctacaa agtctgttgc aggcgctttt
    23761 aaaattgtct cgggattcgc tacctggcga atttctccat cctgcaagac agcaatacgg
    23821 tccgccaact tcaaggcttc atccgtatca tgggttacaa aaatcgttgt catcccaaac
    23881 tctttatgca attcttttgt cagaacctgc aactgttttc tcgaaatagc atccaaggcc
    23941 gaaaagggtt catccatgag gaaaatcttg ggctgaccaa tcatagctcg gacaataccg
    24001 acccgttgct gttctccacc agataattca ctaggtaagc gatgcccata ctcggctact
    24061 ggtaaaccaa ccttagccaa aagctcttct gttttcttcg taatttcttc cttgctccac
    24121 cccttcattt caggaatgag agcaatattt tccgcaactg ttagatttgg aaaaagagca
    24181 atagcctgta aaacataacc agtagaaaga cgaagttcac gctcatcata gtctttgatg
    24241 cgcttcccat ccatataaat atttccatca gttggttcca aaagacggtt aatcatcttg
    24301 agcatggtcg tcttacctga cccagaaggc cctactaaaa ccataaattc cccatcctca
    24361 atctgtaagt tgacatctct caagacatcc ttttctgtgt agcgcagtgc tacatttttg
    24421 tattcaatca ttctttgtcc tcaatttaaa acttccctcg attggtcaag tcttctacct
    24481 taggcatgac ttccttatta tcccaatgct ccacaatttt cccgttctct aaacggaaga
    24541 tatcgtactg ggcataagca acgccatcaa tctgagtctg accatagcta accacatagt
    24601 ttccttgtcc taagagttgg aaaacaaagt caaaagtgac actatattca gccacatagt
    24661 ttttataagc agcacttcct tgtccaatct catgattatg ctgaatcaaa tcgtctgcca
    24721 cataatcact ccactgctct agctccccat tttggaaaat ttctgtcaag aaacggcgaa
    24781 ccagcttttt attttctgct ttcttgtcca aatccttgat ttcaaaatct ccaaaaattt
    24841 gatctagttg gtcattttca ggtgttcgat agtagtcaat gacatcccaa tgctcaacaa
    24901 tacgaccatt ctcatccgca cggaaagtat ccgtcgtcac ccattgagct tctccaccat
    24961 tcagatattg atgaacatga acaaagacca gattgccatc ctcaatggtg cggacaatct
    25021 taatctgacg ctctggatga cgctcaaaga aatctgcaaa gaagactgca aatccttctt
    25081 tcccgtcagg aacacctgtc gaatgttgga tataggtatc ccctacagac tgggcttgag
    25141 cctcagcaac tcgtccgtct tgaatggcat ggatgtatag gttgtgagca tttttcactt
    25201 gttgtgacat attctaaacc tcatttccct tctctttcaa attcgccaaa attctttctt
    25261 gaaaaccttc aaattggtga atttcttcct ctgaaaatcc tttgtaaaag atagtatcca
    25321 atttctgact gacacgatgc cccacttctt tctgggactt gcctaactcc gttaaaacta
    25381 aatacttctt acgcttgtct tttccacacg gactaacaat tacaagcttt tgttcctcta
    25441 gcttttttat catagtcgtc agcgtattat tcgcaagccc agtcgcaagc gcgatatctg
    25501 tcgcagttgc gcagccagtt tcactattcc ataaaaccgc taaaatcttg ccctgttcac
    25561 ccctataaag agcctcagga tcttgactca gtaacttttg aaaaatccgc ccattcaaca
    25621 aacgaatatg atgggctagc aaatgactat ctttcataac acctccaatt tatttcgatg
    25681 tcgaaatgaa taaaataatt gtaacactca tcgttctaac tgtcaactat ttcgatttgg
    25741 aaataatttt tgataattat tcacaccacc atactccggc tcaactaact tttaacgaga
    25801 gtttctaaat tccttcgtcc tccagtctac aaaagccttc catccgtact atcctatttt
    25861 ttatgagggg acacattttt cctatcagat catttatttt aaagatagaa gtaaatcata
    25921 attgcttcca tctgttcttt tatagtatat tgaagttaga ctagggcact gtatcttcta
    25981 aaacattgat agaaagcgat ttgaatttcc caatcaattt gttcgtattt ataacatttt
    26041 gaaactggaa taggacgtta tgactgctaa aatatttcta taaattcatt tgattttcct
    26101 aatcaatttg ttcatatctt atttcattcc gctataattt caccttaccc tatctttttc
    26161 gtagcaccct tcaaacagcc tatcccctac cgtttgacga ttcctcactt cactccactt
    26221 ccattacaga agtttcttca ctactatggt ctcggctgac ttctcatgat tccttgttac
    26281 tactatttga acgctcacga gataggtctt ataaaaaatg ctttgatcca caatggaatc
    26341 aaagcatttt aaaaagttcc tcatacataa gcgcagaagt cgcagttcct ctgtacttgg
    26401 cttcttctct tttgataaag cgagccaagt tgagcaactc aggtgctgga tgtttgggat
    26461 ttaggagcaa ttcacgattg accaggcctg agagacgaac tgcctgcaat tgctcatttg
    26521 tagtaggcag ttttttagta gtctctagga gagtagcaac taaatcttca ctcaaatcat
    26581 gtcgagcatg attgtaaaga tcttttataa ggctttctag gtttggttct accatcccta
    26641 ccacctccct tatggtttaa taatgtttaa tcaaatcaac cgttgaacga tccaatttct
    26701 tcaccaaggc ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca tagagggttt
    26761 ggtgagaatg gatataacga gcgcagacac cgatagttgt tgatgggaca ccaccatttt
    26821 tcagatgagc tgcacctgca tctgttccgc ctttaccaca gtagtattgg tacttgatac
    26881 cagcttcttc agccgttgtc aaaaggaaat ccttcatccc tgggagaagc aagtgacctg
    26941 gatcatagaa acgaatcaag gttccatctc caatcttgcc ttgaccaccg tagacatcac
    27001 ctgctggtga gcaatcaact gcgaggaaga cttctgggtc aaacttggtt gtagaggtat
    27061 gagcgccacg cagaccaact tcttcttgga cgttagaacc cagatagagt tcattgccga
    27121 gtttttgacc tgataaagct tcagctagct cgcttaccat gaggacaccg tagcggttat
    27181 cccaagcttt tgagatgata tttttttcat tggctgtcaa aattgcagaa ctatctggta
    27241 cgatggtatc accaggacgg atgccaaaac tttctgcctc agccttgtcc gcaaaaccac
    27301 catcaaaaac gatatcggca atggctggca tggttggtcc cccctttcca cgagtcaaat
    27361 gcggaggaac agaacctgaa atcacaggaa tttcatgacc atcacgagtc aagagtttga
    27421 aacgttggct gctaaccacc atggggttcc agccaccgat ttctacgaca cggaaggtac
    27481 catctggctt gatttcgctg accataaaac caacttcgtc catatgagaa gcgaccaaga
    27541 cgcgcggtgc atccacagct tctgaatgtt tgataccaaa aataccaccc aagccatctg
    27601 tcaccacttc atccacatgc ggtgtcaact tttcacgaag ataagcacgg acaggcgctt
    27661 catgacctga gactgcagca agttctgtta cttctttaat ttttgaaaat aatgttgtca
    27721 tttcagttcc ttctttcttt catccatttt accacttttt ataggagaag gatagtggga
    27781 aggtggattt ctaagttagt atcttagtcc tgctctatct tagaaaagga tagtattctc
    27841 ttgcatgtag tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt tatttccaaa
    27901 caaaaaaaca atacaccatc aaagttgttt ggatttttca tgaaatttac agaaaatagt
    27961 tgacttccct ttcttctttc tttaaat
//
LOCUS       DAVKZB010000015        27787 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKZB010000015 DAVKZB010000000
VERSION     DAVKZB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.015"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            5..2644
                     /gene="ppc"
                     /locus_tag="VXA80_000578"
     CDS             5..2644
                     /gene="ppc"
                     /locus_tag="VXA80_000578"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV0353119.1"
                     /translation="MILTELLEDITKNMLAPETFEKIIQLKELSTQEDYQGLNRLVTS
                     LSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNIDQDYLGKLSTTIKLVAEKEN
                     AVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRKYRDVKLGLINKDKWYNDLR
                     RYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVPHLTTEYKRLAQAHGLNLKQ
                     AKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNYYDKKIYQLYREFSLSTSIV
                     NVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQATKTYLIEDEEVGTRYETAND
                     FYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYLASIDMRQDSSVYEACVAEL
                     LKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAEKSELLAKELAIFKTARVLK
                     DKLGDDVIHQTIISHATSLSDMLELAILLKEVGLVDTERARVQIVPLFETIEDLDHSE
                     ETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYLSSCWTLYKAQQQLTAIGDE
                     FGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIRLTEQGEVIGNKYGNKDAAY
                     YNLEMLVSAAINRMITQKKSDTNTPNRYEAVMDQVVDRSYDVYRDLVFGNEHFYDYFF
                     ESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWSQSRVMFPGWYGVGSSFKEF
                     INKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNIAFEYAKLCEDEQVKAIYET
                     ILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYFNILNYIQLELIKRQRRGEL
                     SSDQERLIHITINGIATGLRNSG"
     misc_binding    2727..2944
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3057..4061
                     /locus_tag="VXA80_000579"
     CDS             3057..4061
                     /locus_tag="VXA80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0353120.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            4130..4996
                     /locus_tag="VXA80_000580"
     CDS             4130..4996
                     /locus_tag="VXA80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353121.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGYLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTGYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            4993..5751
                     /locus_tag="VXA80_000581"
     CDS             4993..5751
                     /locus_tag="VXA80_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353122.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            5841..7601
                     /gene="dnaG"
                     /locus_tag="VXA80_000582"
     CDS             5841..7601
                     /gene="dnaG"
                     /locus_tag="VXA80_000582"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV0353123.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEEFERLISQKRRME"
     gene            7604..8713
                     /gene="rpoD"
                     /locus_tag="VXA80_000583"
     CDS             7604..8713
                     /gene="rpoD"
                     /locus_tag="VXA80_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV0353124.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            8727..9056
                     /locus_tag="VXA80_000584"
     CDS             8727..9056
                     /locus_tag="VXA80_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0353125.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            9171..10214
                     /gene="cpoA"
                     /locus_tag="VXA80_000585"
     CDS             9171..10214
                     /gene="cpoA"
                     /locus_tag="VXA80_000585"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV0353126.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            10230..11555
                     /locus_tag="VXA80_000586"
     CDS             10230..11555
                     /locus_tag="VXA80_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0353127.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            11586..11762
                     /locus_tag="VXA80_000587"
     CDS             11586..11762
                     /locus_tag="VXA80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0353128.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            11821..11955
                     /locus_tag="VXA80_000588"
     CDS             11821..11955
                     /locus_tag="VXA80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV0353129.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            12019..13329
                     /gene="obgE"
                     /locus_tag="VXA80_000589"
     CDS             12019..13329
                     /gene="obgE"
                     /locus_tag="VXA80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV0353130.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            13339..13497
                     /locus_tag="VXA80_000590"
     CDS             13339..13497
                     /locus_tag="VXA80_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353131.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            14640..15899
                     /locus_tag="VXA80_000591"
     CDS             14640..15899
                     /locus_tag="VXA80_000591"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0353132.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAKGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            15915..16466
                     /locus_tag="VXA80_000592"
     CDS             15915..16466
                     /locus_tag="VXA80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353133.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            16459..17736
                     /locus_tag="VXA80_000593"
     CDS             16459..17736
                     /locus_tag="VXA80_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0353134.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            17752..18612
                     /locus_tag="VXA80_000594"
     CDS             17752..18612
                     /locus_tag="VXA80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV0353135.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            18992..19795
                     /locus_tag="VXA80_000595"
                     /pseudo
     CDS             18992..19795
                     /locus_tag="VXA80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            19986..22277
                     /gene="pcrA"
                     /locus_tag="VXA80_000596"
     CDS             19986..22277
                     /gene="pcrA"
                     /locus_tag="VXA80_000596"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV0353136.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            22320..23000
                     /gene="radC"
                     /locus_tag="VXA80_000597"
     CDS             22320..23000
                     /gene="radC"
                     /locus_tag="VXA80_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV0353137.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(22997..23686)
                     /locus_tag="VXA80_000598"
     CDS             complement(22997..23686)
                     /locus_tag="VXA80_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0353138.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(23704..24345)
                     /locus_tag="VXA80_000599"
     CDS             complement(23704..24345)
                     /locus_tag="VXA80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV0353139.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(24428..24643)
                     /locus_tag="VXA80_000600"
     CDS             complement(24428..24643)
                     /locus_tag="VXA80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0353140.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(24651..24998)
                     /locus_tag="VXA80_000601"
     CDS             complement(24651..24998)
                     /locus_tag="VXA80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV0353141.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(25003..26118)
                     /locus_tag="VXA80_000602"
     CDS             complement(25003..26118)
                     /locus_tag="VXA80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0353142.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(26128..27087)
                     /locus_tag="VXA80_000603"
     CDS             complement(26128..27087)
                     /locus_tag="VXA80_000603"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0353143.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(27200..27769)
                     /locus_tag="VXA80_000604"
     CDS             complement(27200..27769)
                     /locus_tag="VXA80_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV0353144.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
BASE COUNT         8405 a         4984 c         6171 g         8227 t
ORIGIN      
        1 agtcttgatt ctaacagaat tactggaaga tattactaaa aatatgcttg ccccagagac
       61 ctttgaaaaa ataatacagt tgaaagaatt atcaacgcag gaagattatc aaggtctaaa
      121 ccgtctagtg actagcttat caaatgatga aatggtctat atttcacgct atttctctat
      181 cttacctctt ttgattaata tttcagagga tgtggattta gcttatgaaa tcaatcatca
      241 aaataatatt gatcaggact atttaggtaa attatctaca acgattaaat tggtagcaga
      301 aaaggaaaat gccgttgaga tcctagaaca cttgaatgtt gtccctgttt tgacagccca
      361 tccaacacaa gtgcaacgca aaagtatgtt ggatttaaca aatcatattc atagtctttt
      421 gcgtaaatac cgtgatgtta agttggggtt gatcaataaa gataaatggt acaatgattt
      481 gcgtcgttac atcgaaatta tcatgcagac agacatgatt cgtgagaaaa aattaaaagt
      541 gactaacgaa atcacgaatg ctatggaata ttataacagc tcctttttga aagctgtacc
      601 tcatttgacg acggagtata agcgcttagc gcaagcgcat ggtctgaatt taaaacaggc
      661 taaaccaatc accatgggta tgtggatagg tggtgaccgt gatggaaatc catttgttac
      721 agcagagacc ttgaagcagt ctgcactcac tcaatgtgaa gtcatcatga actactatga
      781 taaaaagatt taccaacttt atcgtgaatt ttctctttca actagcattg tcaacgtcag
      841 caagcaagtc agagaaatgg ctcgtcaatc caaggataac tcgatttacc gcgaaaaaga
      901 gctttaccgc cgtgccttgt ttgatattca atcaaaaatt caggcaacta aaacctatct
      961 gattgaggat gaagaagttg ggactcgtta tgaaaccgcc aatgatttct acaaggattt
     1021 gattgccatt cgagattctc tactagagaa taagggcgag tccttgattt caggtgattt
     1081 tgtggaatta ttgcaggcag tagagatatt tggtttttac ctagcatcaa ttgatatgcg
     1141 acaagactct agcgtctatg aagcctgtgt ggcagaactc ttgaaatcag caggaattca
     1201 ttctcgttat agcgagttga gcgaagaaga aaagtgtgac cttcttttga aagaattaga
     1261 agaagatccc cgaattcttt ctgcgactca cgcagaaaaa tcagaattat tagcaaaaga
     1321 actagctatt tttaagacgg ctcgtgtttt gaaagataag ttgggagatg atgtcatcca
     1381 tcagaccatc atttcacatg caaccagcct ttctgatatg ctagaattag ctattctgtt
     1441 aaaagaagta ggactggtgg atacggaaag ggcgcgtgtt cagattgttc ccctttttga
     1501 aacaattgaa gacttggatc attcagagga aacaatgaga aaatatcttt ctcttagtct
     1561 tgccaaaaaa tggattgact cacgaaataa ctaccaagaa atcatgcttg gctactctga
     1621 cagtaataaa gatggcggtt acttgtcatc atgttggacc ctctacaagg ctcaacaaca
     1681 attgactgct attggagatg aatttggcgt taaggttacc ttcttccatg gtcgtggtgg
     1741 tactgtcggt cgtggtggtg ggccaaccta tgaagctatt acatctcaac cgctcaagtc
     1801 tatcaaggat cgtatccgct tgacggagca gggtgaagta attgggaata aatacggtaa
     1861 caaagacgcc gcttactata accttgaaat gctagtatcg gcagctatta accgtatgat
     1921 tactcagaag aagagcgata ccaatacccc aaatcgttat gaagccgtta tggatcaagt
     1981 agtggaccgt agttacgatg tctaccgtga tttggtcttt ggtaatgagc atttctatga
     2041 ttatttcttc gagtcaagtc caatcaaggc tatttcaagt tttaatattg gttctcgtcc
     2101 agccgctcgt aagactatta ctgaaatcgg tggtttgcgt gccatccctt gggtattctc
     2161 atggtcacag agtcgtgtta tgttccctgg atggtacggg gttggttcaa gcttcaagga
     2221 atttatcaat aaaaatccag agaatattgc tatcttacga gatatgtacc aaaattggcc
     2281 tttcttccaa tcgcttcttt caaatgttga tatggttttg tcaaaatcaa atatgaatat
     2341 tgcttttgaa tatgctaaac tttgtgaaga cgagcaagtt aaggccatct atgagactat
     2401 tttaaatgaa tggcaagtta ctaagaacgt tatcttggct attgaaggac atgacgaact
     2461 cttagctgac aatccatatc taaaagctag tctggattac cgtatgcctt actttaatat
     2521 tctcaactat attcagttag agttgattaa acgccaacgt cgtggagaat tgtccagtga
     2581 tcaagaacga ttgattcata tcaccatcaa cggaattgcg acaggattgc gtaattcagg
     2641 ttgataattt tcaagagtga atgctaaaag tgaatatcaa aaaaattcta atagactatt
     2701 gacaagtagt ttaaaaatga tataatttaa ccattcagaa aagtaatcat acaaactttt
     2761 tagagagtct gtggtagctg aaaacagata agtggcaatg atgaaaattg ggctgaatgc
     2821 tatttagaat ttgaaattat aaaaattcgg taagcacacc ttacagtgca tctcgttatt
     2881 gcgagactga gcgataggga aattccctat aattgaggtg gtaccgcgca tcgacgtcct
     2941 cacacaagtt ttttgtgtga ggattttttt gatggaggtt agtatggaaa gaaaacaatg
     3001 gcgtcgcttg tttagataag tgaaatatgt taaaggaaat aaaaaggaga aacagaatga
     3061 aaaataaacg tttaattgga attattgctg cattagcagt cttagtagca ggaagcttga
     3121 tttattcttc aatgaataaa tcagaagttc agaataataa ggatgagaag aaaataacca
     3181 agattggtgt gcttcaattt gtgagccatc catcccttga tttgatttat aaagggatcc
     3241 aagatggact tgcagaagaa ggatataaag atgatcaagt taaaattgat tttatgaact
     3301 cagaaggtga ccaaagtaag gttgcgacaa tgagtaaaca attggttgca aatgggaatg
     3361 accttgtggt tggtatcgca acaccagcag cccaagggtt ggctagtgca acaaaagacc
     3421 taccggttat catggccgct attacagacc caattggtgc taacttggtt aaagatttga
     3481 aaaaaccagg tggcaacgtt acaggggtat ctgaccacaa tccagctcaa caacaagttg
     3541 aactcatcaa ggctctgaca ccgaatgtga aaacaatcgg agctctttac tcaagtagcg
     3601 aagacaattc aaaaacacag gtcgaagaat ttaaggctta tgctgaaaaa gcaggtctga
     3661 cagtggaaac atttgcagtt ccttcaacaa atgaaattgc ctcaactgtc actgttatga
     3721 ctagcaaggt agatgctatt tgggttccaa ttgataacac cattgcatca ggatttccaa
     3781 cggttgtctc tagcaatcaa agttctaaga aaccaattta tcccagtgcg acagctatgg
     3841 tagaagtagg tggtttggca tcagttgtaa ttgaccaaca tgaccttggt gtggcaacag
     3901 gtaaaatgat tgtgcaagtc ttgaaaggtg caaaaccagc cgatacccca gtcaatgtct
     3961 tttcaactgg taagtcagtc atcaataaaa aaatagcaca agaactaggt attactattc
     4021 ctgagtctgt tctcaaagaa gcaggacaag tcatcgaata aataaggtag ggaggaggag
     4081 actcttccca ttttttaaaa agatgaatat agaaaggatt aaataaaata tgattgtttc
     4141 cattatttct caaggatttg tctgggctat tctaggtctg ggaatcttta tgacatttag
     4201 gattttaaac tttccagata tgacgacaga aggttccttc cctcttgggg gagctgttgc
     4261 tgtcactttg ataaccaaag gcgtgaatcc atttttagcg acacttgttg ctgtaggagc
     4321 aggttatttg gctggaatgg cagcaggcct tctttataca aaagggaaga tcccaacctt
     4381 gctctcaggg attttggtga tgacttcttg tcactcaatc atgctcttga ttatgggacg
     4441 tgcaaattta ggcctgcttg gaaccaagca aattcaggat gttttgcctt ttgattcgga
     4501 tttgaatcaa ctcttgacag gtctcatctt tgtgagcatt gttattgctc tcatgctctt
     4561 tttcttggac actaaactcg gacaagccta tattgctaca ggggataatc ctgatatggc
     4621 tagaagtttc gggattcata ctggacgcat ggagctcatg ggcttggtct tatcaaatgg
     4681 tgtgattgcc cttgcaggtg ccctcattgc tcagcaagaa ggctatgccg atgtgtctcg
     4741 agggatcggg gttatcgttg tggggcttgc aagtttgatt attggagaag ttattttcaa
     4801 gagtttgagc ttggcagagc gtttggttac tatcgttgta ggttctatcg cttatcaatt
     4861 tttagtgtgg gcagttatcg cacttggctt taatacaggt taccttcgtt tatacagtgc
     4921 tttgatttta gcagtctgcc tcatgattcc aacatttaag caaacaatct tgaaaggagc
     4981 caagttaagc aaatgacagc aattgtagaa ttaaaaaatg caaccaaaat cgttaaaaat
     5041 ggctttgatg aagaaaagat tattttaaat gatgtttcct tagaaatttt tgaacgggac
     5101 tttatcacga ttttgggcgg aaatggtgct ggaaaatcaa ctctctttaa cactatagca
     5161 gggaccttat cactaactag tggaactatc cgtattttag gtgaagatct cactaagttt
     5221 tcacccgaga agcgtgccaa gtacctgtct cgtgtcttcc aagatccaaa gatggggaca
     5281 gctccccgta tgacggtcgc tgaaaatctt ttaatcgcca agtttcgtgg tgaaaagcgt
     5341 ggattgttac caagacgctt gactagctat aaggatgaat ttcaggcaac cattgaaaaa
     5401 gtaggaaatg gtcttgagaa acacttgaat acaccgattg agttcttatc aggtggacaa
     5461 agacaggctt tgagtctctt gatggcaacc ttgaagcgac ctgaattact cctgttagat
     5521 gagcatactg ctgccctgga tccaaagact agtgttgctt tgatggaatt gacagatgaa
     5581 tttgttaaga aagatcagct aacagccctt atgattactc atcatatgga agatgctctc
     5641 aaatacggca atcgcttgat tgtcatgaaa gaaggacgga ttatccaaga tttaaaccaa
     5701 gaagaaaaag caaaaatgaa aatctctgat tattatcaac tctttgaata aagtagggaa
     5761 aatgagtgaa atggtttact ttttttctga aataaagtat actatataaa gtaaactatg
     5821 ataacatgga ggtattgtgt atggttgaca aacaagtcat tgaagaaatc aaaaacaatg
     5881 ccaacattgt ggaagtcata ggagatgtga tttctttaca aaaggcagga cggaactatc
     5941 tagggctctg tccttttcat ggtgaaaaaa caccttcttt caacgttgta gaggataagc
     6001 agttttacca ctgttttggt tgtggtcgtt caggtgatgt ctttaaattc atcgaggagt
     6061 accaaggggt tacctttatg gaggctgtcc aaatcttagg tcagcgtgtc gggattgagg
     6121 ttgaaaaacc gctttatagt gaacagaagc cagcctcgcc tcaccaagct ctttatgata
     6181 tgcacgaaga tgcggctaaa ttttaccatg ctattctcat gacaacgact atgggcgaag
     6241 aggccagaaa ttacctttat cagcggggtt tgacagatga agtgcttaaa catttttgga
     6301 ttggtttagc acctccagaa cgaaactatc tctatcaacg tttgtctgat cagtatcgtg
     6361 aagaggattt actggattca ggcctgtttt atctctcgga tgccaatcaa tttgtagaca
     6421 cctttcacaa tcgcattatg tttcccctga caaatgacca aggaaaggtc attgccttct
     6481 caggtcgtat ctggcaaaaa acggattcac aaacttctaa gtataaaaac agccgatcga
     6541 ctgcaatttt taacaaaagt tacgaattat atcatatgga tagggcaaaa aaatcttctg
     6601 gaaaaactag tgagatttac ctgatggaag gattcatgga tgttattgca gcctatcggg
     6661 ctggaatcga aaatgctgtg gcgtcgatgg gaacggcctt gagtcgagag catgttgagc
     6721 atctgaaaag gttaaccaag aaattggttc ttgtttacga tggagataag gctgggcaag
     6781 ccgcgatatt gaaagcattg gatgaaattg gtgatatgcc tgtgcaaatc gttagcatgc
     6841 ctgataactt ggatcctgat gagtatctac aaaaaaatgg tccagaagac ttggcctatc
     6901 tattaacaaa aactcgtatt agtccgattg agttctacat tcatcagtac aaacctgaaa
     6961 acagtgaaaa tctgcaggct cagattgagt ttattgaaaa aatagctccc ttgattgttc
     7021 aagaaaagtc catcgctgct caaaacagct atattcatat tttagctgac agtctggcgt
     7081 cctttgatta tacccagatt gagcagattg ttaatgagag tcgtcaggtg caaaggcaga
     7141 atcgcatgga aagaatttcc agaccgacgc caatcaccat gcctgtcacc aagcagttat
     7201 cggctattat gagggcagaa gcccatctac tctatcggat gatggaatcc cctcttgttt
     7261 tgaacgatta ccgtttgcga gaagactttg catttgctac acctgaattt caggtcttat
     7321 atgacttgct tggccagtat ggaaatcttc ctccagaagt tttagcagag cagacagagg
     7381 aagttgaaag agcttggtac caagttttag ctcaggattt gcctgctgag atatcgccgc
     7441 aggaacttag tgaagtagag atgactcgaa acaaggctct cttgaatcag gacaatatga
     7501 gaatcaaaaa gaaggtgcag gaagctagcc atgtaggaga tacagataca gccttagaag
     7561 aatttgaacg tttaatttcc caaaagagaa gaatggagta ataatggcaa caaaacaaaa
     7621 agaagtaaca acatttgacg tacaggtagc agaatttatc cgtaatcata agcaaaaagg
     7681 gacagcaaca gatgatgaaa tcaatgcaag tctggttatt ccttttacct tggacgctga
     7741 tgggattgaa gatctcttgc aacggattca ggatgcaggc atttctatca cagataacga
     7801 aggaaatcca agtgcgcgtg ttcttagcaa tgaagaagaa ccagaactca gcgatgagga
     7861 cttgattggg tcaacttctg ctaaggtcaa tgaccctgtc cgtatgtact tgaaagaaat
     7921 aggggtcgtt cctctcttga ccaatgaaga ggagaaagag ttggcactgg ctgttgaagc
     7981 tggcgatatc gaagccaaac aacgtcttgc ggaagccaat cttcgtttgg ttgtttccat
     8041 tgccaaacgc tatgtcggtc gtggcatgca gttccttgac ttgattcaag aaggaaatat
     8101 gggcttgatg aaggcggttg acaagtttga ctattctaaa gggttcaagt tttcaactta
     8161 tgcaacttgg tggattcgtc aggctatcac tcgtgctatt gcagaccaag ctcgtaccat
     8221 ccgtatccca gttcacatgg ttgaaactat caataaattg gttcgtgaac agcggaatct
     8281 ccttcaagaa ttggggcaag atccgacacc agaacagatt gctgaacgaa tggatatgac
     8341 acctgataag gttcgtgaaa tcttgaagat tgcccaagaa ccagtatctc ttgaaactcc
     8401 tatcggtgaa gaggacgata gccaccttgg agactttatc gaagatgaag tgattgaaaa
     8461 tccagtggat tatacgactc gtatcgtctt gcgtgagcaa ttggatgaaa tcttagatac
     8521 tcttacagac cgtgaagaaa atgttctgcg tctacgtttt ggactagatg atggaaaaat
     8581 gcgcacactt gaagatgtgg ggaaagtctt taacgtaact cgtgagcgta tccgtcagat
     8641 tgaagcaaag gctttgagaa aactacgcca accaagtcgt agcaaaccgc ttcgtgattt
     8701 tattgaagac taagagtgag gaaaatatgg cttatacaga agagcaaatt gaaaacatca
     8761 aaacacggat tttaacagcc ttggaagaag tcatcgaccc tgagttggga atcgatattg
     8821 tcaatcttgg tttgatctat gagattcgtt ttgacggtga cacagggcaa acagagattg
     8881 acatgacttt gacaactatg ggttgtccct tagcagacct tttgacagac cagatttatg
     8941 atgctatgat agaggttcca gaagtaacgg atactgaggt taaattagtc tggtatccag
     9001 cctggacagt tgaaaaaatg agtcgctatg cccgcattgc cctaggcatt aagtaaacat
     9061 ataaaagcat gtgagagact gttggaaaag cgaggaaatt tcccctcttt tcctctagtc
     9121 tctcctttct tttgctgatt ttattcaaag aaaatgatat aatagtagtt atggagaaaa
     9181 agaaattacg catcaatatg ttgagttcaa gtgagaaagt agcaggacag ggagtttcag
     9241 gtgcttaccg tgaattagtt cgtcttcttc accgtgctgc caaggaccaa ttgattgtta
     9301 cagaaaatct tccaatcgag gcagatgtga ctcactttca tacgattgat tttccctatt
     9361 atttatcaac cttccaaaag aaacgctcag ggagaaagat tggctatgtg catttcttgc
     9421 cagctacact tgagggaagt ttgaaaattc catttttctt aaagggaatt gtgaaacgct
     9481 atgtattttc tttttacaac cggatggagc acttggttgt ggtcaatcct atgtttattg
     9541 aggatttggt agcagctggt attccacgtg aaaaagtgac ctatattcct aactttgtca
     9601 acaaggaaaa atggcatcct ctaccacaag aagaggtagt caggctgcgc acagatcttg
     9661 gtcttagtga caatcagttt atcgtagtag gtgctgggca agttcagaaa cgtaaaggga
     9721 ttgatgactt tatccgtctg gctgaggaat tgcctcagat tacctttatc tgggctggtg
     9781 gcttctcttt tggtggtatg acagatggtt atgaacacta taagaaaatt atggaaaatc
     9841 cccctaaaaa tttgattttt ccaggcattg tatcgccaga gcggatgcgc gaattgtatg
     9901 ctctagcgga tcttttcttg ttgcctagtt acaatgagct ctttcctatg actattttag
     9961 aagctgcgag ttgtgaggct cctattatgt tgcgtgattt agatctctat aaggtgattt
    10021 tggagggaaa ttatcgggcg acagcgggta gagaagagat gaaagaggct attttggaat
    10081 atcaagcaaa tcctgctgcc ttaaaagatc tcaaagaaaa ggctaagaat atttccagag
    10141 agtattctga agagcatctg ttacaaatct ggttggactt ttatgagaaa caagccgctt
    10201 tagggagaaa gtaaaaagtg aggtaatcta tgcgaattgg tttatttaca gatacctatt
    10261 ttcctcaggt ttctggtgtt gcgaccagta ttcgaacctt gaaaacagaa cttgaaaagc
    10321 agggacatgc tgtttttatc tttacgacga cagataagga tgtcaatcgc tacgaagatt
    10381 ggcaaattat ccgcattcca agtgttcctt tctttgcttt taaggatcgt cgctttgcct
    10441 accgaggttt tagcaaggca cttgaaattg ctaaacagta tcagctagat attatccata
    10501 ctcagacaga attttctctt ggcctgttgg ggatttggat tgcgcgtgaa ttgaaaattc
    10561 cagtcatcca tacctatcac acccagtatg aagactatgt ccattatatt gctaagggga
    10621 tgttgatccg gccgagtatg gtcaagtatc tggttagagg tttcctgcat gatgtggatg
    10681 gggttatttg ccctagtgag attgtccgtg acttgctatc tgattataag gtcaaggttg
    10741 aaaaacgggt cattcctact gggattgaat tagccaagtt tgagcgtccg gaaatcaagc
    10801 aggaaaattt gaaagaactg cgtagtaaac tagggattca agatggtgaa aagacgttgc
    10861 ttagtctttc gagaatctcc tatgaaaaaa atattcaagc agttttagca gcctttgctg
    10921 atgttctgaa agaggaagac aaggttaaac tggtagtagc tggggatggc ccttatctga
    10981 atgacctcaa agagcaagcc cagaacctag agattcaaga ctcagtcatc tttacaggga
    11041 tgattgctcc tagtgagacg gctctttact ataaagcggc ggatttcttc atttcggcat
    11101 cgacaagcga aacgcaaggt ttgacctact tggaaagctt agccagtgga acacctgtca
    11161 ttgctcacgg aaatccttat ttgaacaacc tcatcagtga taaaatgttt ggaaccttgt
    11221 actatggaga acatgatttg gctggtgcta ttttggaagc cctgattgca acaccagaca
    11281 tgaacgagca taccttatca gagaaattgt atgagatttc agctgagaac tttgggaaac
    11341 gagtgcatga gttttatctg gatgccatta tttcaaataa cttccagaaa gatttggcta
    11401 aagatgatac ggtcagtcag cgtatcttta agacagtttt gtatcttcag caacaggtgg
    11461 ttgctgtacc tgtaaaagga tctagacgca tgttgaaggc ttcaaaaaca cagttgatca
    11521 gtatgagaga ctattggaaa gaccatgaag aatagaaaga ggaacagcta tgaaaaaaac
    11581 aattaatgag aagcggtcgt gataaaaaga ttgcgggtgt ttgtgctggg gtggcccatt
    11641 atctggatat ggatccgact atcgttcaag tcatttgggg tgttcttact tgctgttacg
    11701 gagctggaat tgtagcttac attattttat ggattatcgc accagtagca actgactatt
    11761 gaaaactagc tcaattcatg ctaagataat agaaaataga atgtaacgaa ggagagaaaa
    11821 atggcatttg gagataatgg aaatcgtaaa aaaactatgt ttgagaaaat aaccttgttt
    11881 atcgtgatta tcatgctagt agcaagttta ttgggaattt ttgcaactgc aattggtgcc
    11941 ctcagtaatc tataaaatag attcaagaaa atttagtgac tgggatttcc cagccctttt
    12001 ttaaagtgag aagaaataat gagtatgttt ttagatacag ctaagattaa ggtcaaggct
    12061 ggtaatggtg gcgatggtat ggttgccttt cgtcgtgaaa aatatgtccc taatggaggc
    12121 ccttggggtg gtgatggtgg tcgtggaggc aatgtggtct tcgttgtaga cgaaggacta
    12181 cgtaccttga tggatttccg ctacaatcgt catttcaagg ctgattctgg tgaaaaaggg
    12241 atgaccaaag ggatgcatgg tcgtggtgct gaggacctta gagttcgagt accacaaggt
    12301 acgactgttc gtgatgcgga gactggcaag gttttaacag atttgattga acatgggcaa
    12361 gaatttatcg ttgcccacgg tggtcgtggt ggacgtggaa atattcgttt cgcgacacca
    12421 aaaaatcctg caccggaaat ctctgaaaat ggagaaccag gtcaggaacg tgagttacaa
    12481 ttggaactaa aaatcttggc agatgtcggt ttagtaggat tcccatctgt agggaagtca
    12541 acacttttaa gtgttattac ctcagctaag cctaaaattg gtgcctacca ctttaccact
    12601 attgtaccaa atttaggtat ggttcgcacc caatcaggtg aatcctttgc agtagccgac
    12661 ttgccaggtt tgattgaagg ggctagtcaa ggtgttggtt tgggaactca gttcctccgt
    12721 cacatcgagc gtacacgtgt tatccttcac atcattgata tgtcagctag cgagggccgt
    12781 gatccatatg aggactacct agctatcaat aaagagctgg agtcttacaa tcttcgcctc
    12841 atggagcgtc cacagattat tgtagctaat aagatggaca tgcctgagag tcaggaaaat
    12901 cttgaagact ttaagaaaaa attggctgaa aattatgatg aatttgaaga gttaccagct
    12961 atcttcccaa tttctggatt gaccaagcaa ggtctggcaa cacttttaga tgctacagct
    13021 gaattgttag acaagacacc agaatttttg ctctacgacg agtccgatat ggaagaagaa
    13081 gcttactatg gatttgacga agaagaaaaa gcctttgaaa ttagtcgtga tgacgatgcg
    13141 acatgggtac tttctggtga aaaactcatg aaactcttta atatgaccaa ctttgatcgt
    13201 gatgaatctg tcatgaaatt tgcccgtcag cttcgtggta tgggggttga tgaagccctt
    13261 cgtgcgcgtg gagctaaaga tggggatttg gtccgcattg gtaaatttga gtttgaattt
    13321 gtagactagg agactggtat gggagataaa ccgatatctt tccgagatgc ggatggtaat
    13381 tttgtttccg ccgcagacgt ttggaatgaa aagaaattgg aagaactatt taatcgtctc
    13441 aatccaaatc gtgccttgag attggcacga actaaaaagg aaaatccatc tcagtaaaga
    13501 agctaaaaaa tcccgtgcct catcagacac gggattttgt ggtacgacag gcatgtatag
    13561 caaactgaat ctggaatagc acagcatatc ttctaaaata tagtaaaatg aaatgagaac
    13621 aggacaaatc gatcaggaca gtaaaatcga tttctaacaa tgttttagaa gcagagatgt
    13681 actattctag tttcaatcaa ctatattgtt ataaattgat ttgaatttca aaattaaatt
    13741 gtttgattct tatttcaatt tgttatagta tatctgatgt caaagttctc ggcgagtcaa
    13801 atagcgattc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct ggggatagac
    13861 cgttttaagt ctgacgctgg aaataagaat tgtcagaaga agggatagcg aaatcgtggc
    13921 tctacgaaca ggaacgtgat aataaggcgt atatagcgga taagagggca tcaaactcta
    13981 aagtccaaaa aggtagtcgt aacctatatg cgtaaatcac gagagtaatt gaattcgtac
    14041 taagattttc tattttcact gtaacctttt aacgccctta tatcttgtat acacgaggaa
    14101 agatgtacga cttatcccgt gaggtctatc actataaaga gaaaacgaca gatagaagtg
    14161 atcctgagtc acggttatct gtctgatagg acggtatgta taaaacgctt ctgtgaactg
    14221 agagaagggg gagaagttct tgctaaaatt tagttgaaca gccgtattcc gatacttaga
    14281 taagagatct agtcttagct cctactcagt tttaggggat aaaaaagggg caatagcgat
    14341 tcgagaaaga ttatactctt cgaaaatctc ttcaaatcac gtcaatatcg ccttgtcgta
    14401 tgtgtaggat actgactacg tcagttccat ctacaacctc aaaacagtgt tttgagcaac
    14461 ctgcggctag tttcctagtt tgatctttga ttttcattga gtattagtaa ttcagttact
    14521 aactcgtcaa ctctgattta tccaataaaa ttgaaaagga tggaaaaaag gataaattta
    14581 tgatatactt tattttgaag accttattag aaatcttgaa agagtattga aaacttagaa
    14641 tgagaaaaat tgttatcaat ggtggattac cactgcaagg tgaaatcact attagtggtg
    14701 ctaaaaatag tgtcgttgcc ttaattccag ctattatctt ggctgatgat gtggtgactt
    14761 tggattgcgt tccagatatt tcggatgtag ccagtcttgt cgaaatcatg gaattgatgg
    14821 gagctactgt taagcgttat gacgatgtat tggagattga cccaagaggt gttcaaaata
    14881 ttccaatgcc ttatggtaaa attaacagtc ttcgtgcatc ttactatttt tatgggagcc
    14941 tcttaggccg ttttggtgaa gcgacagttg gtctaccggg aggatgtgat cttggtcctc
    15001 gtccgattga cttacacctt aaggcgtttg aagctatggg tgccactgct agctacgagg
    15061 gagataacat gaagttatct gctaaagata caggacttca tggtgcaagt atttacatgg
    15121 atacggttag tgtgggagca acgattaata cgatgattgc tgcggttaaa gcaaatggtc
    15181 gtactattat tgaaaatgca gcccgtgaac ctgagattat tgatgtagct actctcttga
    15241 ataatatggg tgcccatatc cgtggggcag gaactaatat catcattatt gatggtgttg
    15301 aaagattaca tgggacacgt catcaggtga ttccagaccg cattgaagct ggaacatata
    15361 tatctttagc tgctgcagtt ggtaaaggaa ttcgtataaa taatgttctt tacgaacacc
    15421 tggaagggtt tattgctaag ttggaagaaa tgggagtgag aatgactgta tctgaagaca
    15481 gcatttttgt cgaggaacag tctaatttga aagcaatcaa tattaagaca gctccttacc
    15541 caggctttgc aactgatttg caacaaccgc ttacccctct tttactaaga gcgaatggtc
    15601 gtggtacaat tgtcgatacg atttacgaaa aacgtgtaaa tcatgttttt gaactagcaa
    15661 agatggatgc ggatatttcg acaacaaatg gtcatatttt gtacacgggt ggacgtgatt
    15721 tacgtggggc cagtgttaaa gcgaccgact taagagctgg ggctgcacta gtcattgctg
    15781 ggcttatggc taaaggtaaa actgaaatta ccaatatcga gtttatctta cgtggttatt
    15841 ctgatattat cgaaaaatta cgtaatttag gagcggatat tagacttgtt gaggattaaa
    15901 ccgtagaggt gtttatgaat atttggacca aattagcaat gttttctttt tttgaaacgg
    15961 atcgcttgta tttgcgtcct ttctttttta gtgatagtca ggacttccgc gagatagctt
    16021 caaatccaga aaatcttcaa tttattttcc caacgcaggc aagtctggaa gaaagtcaat
    16081 atgcactggc caattacttt atgaagtccc ctttgggagt gtgggcaatt tgtgaccaga
    16141 aaaatcaaca aatgattggt tctattaaat ttgagaagtt agatgaaatc aaaaaagaag
    16201 ctgagcttgg ctattttttg agaaaagatg cttggtcgca aggatttatg acagaggttg
    16261 ttagaaaaat ttgtcagctt tcttttgagg aatttggctt aaaacaatta tttatcatta
    16321 cccaccttga aaataaagct agccaaagag ttgctcttaa gtctggattt agtttgttcc
    16381 gtcagtttaa gggaagtgat cgttacacaa gaaaaatgcg ggattatctt gaatttcggt
    16441 atgtaaaagg agagttcaat gagtaagcat caggaaattc taagctattt ggaggaatta
    16501 ccagtaggta aaagggtcag tgttcgtagc atttcgaatc atctaggagt tagtgatgga
    16561 acagcctatc gggctattaa agaagctgaa aaccgtggaa ttgtggagac ccgtcctaga
    16621 agtggaacaa ttcgtgttaa atcccagaaa gttgctatag agagattaac gtttgctgaa
    16681 attgcagaag tgacttcttc tgaggttctg gctgggcaag aaggtttaga gagagaattt
    16741 agtaagtttt caattggtgc catgactgaa caaaatatct tgtcttacct tcatgatggg
    16801 gggctcttga ttgtcggaga ccgaacccgt attcagttgc tagccttgga aaatgaaaat
    16861 gcagttctgg ttacaggggg atttcaggtt catgatgatg tgcttaaact ggccaatcaa
    16921 aaagggattc ctgttctaag aagtaagcat gataccttta ccgtcgcgac catgatcaat
    16981 aaagccttgt caaatgtcca aatcaagact gatattctga cagttgagaa actttatcgc
    17041 cctagtcatg agtatggttt tctgagagag acagatacag ttaaagatta tttggacttg
    17101 gttcgtaaga atcgtagcag ccgtttccct gttatcaatc aacatcaggt cgttgttggt
    17161 gttgtaacca tgagagacgc tggtgataaa tcaccaagca cgacaattga taaggttatg
    17221 tctcgtagtc tatttttggt tggattatcg acaaatattg ccaatgtgag tcaacggatg
    17281 atcgcagaag actttgaaat ggtaccagtt gttcgaagca atcaaacttt gcttggcgtt
    17341 gtgacgcgac gagatgtcat ggagaagatg agccgttccc aagtttcggc tctaccaact
    17401 ttttctgagc agattggaca aaagctctct tatcaccatg atgaagtagt cattacagtg
    17461 gaacccttta tgctagaaaa aaatggagtt ttggctaatg gtgtattggc agaaattctg
    17521 acccacatga cccaagattt agttgttaat agtggtcgca atctcattat cgagcagatg
    17581 ctgatctact ttttgcaggc tgttcagata gatgatatat tgcgcattca ggcacggatt
    17641 attcatcata cgagacggtc agctataatt gattacgata tttatcatgg tcaccagatt
    17701 gtttcaaaag caaatgtgac tgttaaaatt aattagaaac taggagaaaa gatgataaca
    17761 ttaaaatcag ctcgtgaaat cgaagctatg gacaaggctg gtgattttct agcaagtatt
    17821 catataggct tacgtgattt gattaagcca ggcgtagata tgtgggaagt tgaagaatat
    17881 gtccgccgtc gttgtaaaga agaaaatttc cttccacttc agattggggt tgacggtgcc
    17941 atgatggact atccttatgc tacctgttgc tctcttaacg atgaagtggc tcacgctttc
    18001 cctcgtcatt atatcttgaa agatggtgat ttgctcaaag ttgatatggt tttgggaggt
    18061 cccattgcta aatctgacct gaatgtctca aaattaaact tcaacaatgt tgaacaaatg
    18121 aaaaaataca ctcagagcta ttctggtggt ttagcagact catgttgggc ttatgctgtt
    18181 ggtacaccgt ccgaagaagt caaaaacttg atggatgtaa ccaaagaagc tatgtacaag
    18241 ggtattgagc aagctgttgt tggaaatcgt atcggtgata tcggtgcggc tattcaagaa
    18301 tacgctgaaa gtcgtggtta cggtgtagtg cgtgatttgg ttggtcatgg tgttggccca
    18361 actatgcacg aagaaccaat ggttcctaat tatggtattg caggtcgtgg actccgtctt
    18421 cgtgaaggaa tggtcttaac cattgaacca atgatcaata caggcgattg ggaaattgat
    18481 acagatatga aaactggttg ggcgcataag accattgacg gtggattgtc atgtcagtat
    18541 gaacaccaat ttgtcattac gaaagatgga cctgttatct tgactagcca aggtgaagaa
    18601 ggaacttatt aataaaaagt gaaaagacta ctggaagttt attttgataa aaaatccagt
    18661 agatcttttc ataataaaac gcattgtatc aagtgttagg ggctgatatc atgcgttttt
    18721 ctgcttttaa gattttttcc aactctgttt gtaagcgcat cataacaaag ggtctaggat
    18781 tcagggctct cctcctatat actattagta aagtaaaact aagggaggat attttagtgt
    18841 cgcagtctat tgttcctgta gagattccac aatattgtcg ttttgattct aaaaagagaa
    18901 atggaattct gtttaatgtt cgtattgcca atcttaaatt tactttttta tattatactt
    18961 cctgcgaaac aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac
    19021 tgatgcacga tttaagcgtc ttgttggtgt tcagcgtacc acttttgaag agatgttagc
    19081 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
    19141 cctagaagac cttcttatgc ccactcttca atagtgcgag aatatcgaac ttatgaagaa
    19201 attgcggctg attttggtat tcacgaaagc aactttatcc gtcggagcca atgggttgaa
    19261 ataactcttg ttcaaagtgg ttttacggtt tcaagaactc ctctcagttc tgaggacacg
    19321 gtaatgattg atgcgacgga agtaaaaatc aatcgcccta aaaaaacaat tagcgaatga
    19381 ttctggtaaa aagaaatttc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
    19441 aattgtttct ttggatatcg ctgtgaacta tagtcatgat atgaagttgt tcaaaatgag
    19501 tcgtagaaat atcgaacaag ctggtaaaat cttggctgac agtggttatc aagggctcat
    19561 gaagatatat cctcaagcac aaactccacg taaatccagc aaactcaagc cgctaacagc
    19621 tgaagataaa gcctataacc atgcgctatc taaggaaaga agcaaggttg agaacatctt
    19681 tgccaaagta aaaacgttta aaatattttc aacaacctat cgaaatcatc gtaaacgctt
    19741 cggattacga atgaatttga gtgctggtat tatcaatcat gaactaggat tctagttttg
    19801 caggaagtct attgaggtat tgagctagtt tatgaaaaaa ttgggtgaaa agtcgagtgt
    19861 tttagaaacc cacagtgtag tattctagtt tcaatccact atattttgct actccccgta
    19921 aagtttctat tttccctgat ttctgatata atagaaatat tgacttcaag agtaaggaag
    19981 agaagatgaa cgcattatta aatggaatga atgaccgtca ggctgaggcg gtgcaaacga
    20041 cagaaggtcc cttgctaatc atggcagggg ctggttctgg aaagactcgt gttttgaccc
    20101 accgtatcgc ttatttgatt gatgaaaagc tggtcaatcc ttggaatatc ttggccatta
    20161 cctttaccaa caaggctgcg cgtgagatga aagagcgtgc ttatagcctc aatccagcga
    20221 ctcaggactg tctgattgcg accttccact ccatgtgtgt gcgtattttg cgtcgcgatg
    20281 cggaccatat tggctacaat cgtaatttta caattgtgga tcctggtgaa cagcgaacgc
    20341 tcatgaaacg tattctcaaa cagttgaact tggaccctaa aaaatggaat gaacgaacta
    20401 ttttggggac catttccaat gctaagaatg atttgattga tgatgttgct tatgctgccc
    20461 aagctggcga tatgtatacg caaattgtgg cccagtgtta tacagcctat caaaaagaac
    20521 ttcgtcagtc tgaatccgtt gactttgatg atttgattat gctgaccttg cgtctctttg
    20581 atcaaaatcc tgatgttttg acctactacc agcaaaaatt ccaatacatc cacgttgatg
    20641 agtaccaaga taccaaccac gctcagtacc aattggtcaa actcttggct tcccgtttta
    20701 aaaatatctg tgtggttggg gatgcggacc agtctatcta cggttggcgt ggtgctgata
    20761 tgcagaatat cttggacttt gaaaaggatt accccaaagc caaggttgtt ttgttggagg
    20821 aaaattaccg ctcaaccaaa accattctcc aagcggccaa cgaggttatt aaaaataata
    20881 aaaatcgccg tcctaaaaat ctctggactc aaaacgctga tggggagcaa atcgtttact
    20941 atcgtgccga tgatgagctg gatgaggctg tatttgtagc cagaaccatc gatgaactta
    21001 gtcgcagtca aaacttcctt cataaggatt ttgcagttct ctatcggact aatgcccagt
    21061 cccgtacaat tgaggaagcc ctgctcaagt ctaacattcc ttataccatg gttggcggaa
    21121 ccaaattcta cagccgtaag gaaattcgcg atattattgc ttatctcaac cttattgcta
    21181 atttgagtga caatattagt tttgagcgta ttatcaacga gcctaaacgt ggaattggtc
    21241 taggtacagt tgagaaaatc cgtgattttg caaatttgca aaatatgtct atgctggatg
    21301 cttctgctaa tattatgttg tctggtatca agggtaaggc agcccaatct atctgggatt
    21361 ttgccaatat gatgcttgat ttgcgggagc agctagacca cttaagcatt acagagttgg
    21421 ttgagtccgt cctagaaaaa acaggttatg tcgatattct taacgcccaa gcgactctag
    21481 aaagcaaggc acgggttgaa aatatcgaag agtttctttc tgttacgaag aactttgatg
    21541 acaccacgga tgtgacagaa gaggaaactg gtctggacaa actgagtcgt ttcttaaatg
    21601 acttggcttt gattgccgac acagattcag gtagtcagga gacatcagaa gtgaccttga
    21661 tgaccctgca tgctgccaaa ggtctcgaat ttccagttgt ctttttgatt gggatggaag
    21721 aaaatgtctt tccacttagt cgtgcgactg aagattcaga tgaattagaa gaagagcgcc
    21781 gtctagccta tgtaggtatc acgcgtgcag agaaaattct ctatctgacc aatgccaact
    21841 cacgcttgct ttttggtcgt accaattata accgtccgac tcgttttatt aacgaaatca
    21901 gttcagactt gcttgagtat caaggtctgg ctcgtcctgc aaatacaagc tttaaggcat
    21961 catatagcag tggtagtatt tcctttggtc aaggtatgag tttggctcag gctcttcaag
    22021 accgtaaacg cggtgctgcc ccaaaatcaa tccagtcaag cggtcttcca tttggtcaat
    22081 ttacagctgg cgcaaaacca gcatctagcg aggcaaattg gtccattggt gatattgctc
    22141 tccacaagaa atggggagag ggaaccgttc tggaagtttc aggtagcggt gctaggcagg
    22201 aattgaaaat caatttccca gaagtaggtt tgaaaaaact tttagccagt gtggctccaa
    22261 ttgagaaaaa aatctaattt tccatccttc tcacgaataa taaagtgagg aggattttta
    22321 tgtacagtat ttcattccaa gaagattcac tattaccaag agaaaggctg gccaaggaag
    22381 gagttgaagc gcttagtaac caagagttgc tagctatttt actcaggaca ggaacacgtc
    22441 aagctagcgt ttttgaaatt gcccaaaaag tcttgaacaa tctttcaagc ctaacggatt
    22501 tgaaaaaaat gaccctgcag gaattgcaga gtttgtctgg tattgggcgt gttaaggcca
    22561 tagaattaca agctatgatt gaactggggc atcgtattca caaacacgag actcttgaaa
    22621 tggaaagtat tctcagcagt caaaagttgg ccaagaagat gcagcaggaa ttaggggata
    22681 aaaaacaaga gcacctggtg gcactctatc tcaatactca aaatcaaatc atccatcagc
    22741 agaccatttt tatcgggtct gtaactcgta gtatcgctga accgcgagag attcttcact
    22801 atgcaatcaa gcatatggcg acttctctta tcttggtcca caatcatcct tcaggagcgg
    22861 tagcgcctag ccaaaatgat gatcatgtca ctaaacttgt taaagaagcc tgcgaattga
    22921 tggggattgt tctcttggac catttgattg tctctcattc taattacttt agttatcgtg
    22981 aaaagacaga tttaatctaa agttcattaa cgacatagtc aaagagtttt ttatctttgg
    23041 gacgattttc aaaaagaagt tctggatgcc attggacacc gagaaaggcg acatcatccg
    23101 tactcatgac agcctcaatg ataccatctt taggatcatg agccacaact tttaaatttg
    23161 gtgctaagtc cttgatgctc tggtggtgga aggagttgat atgagagatt tctccataga
    23221 tttcttggag aacggtatct ggttctgtta ccaagcgttg agttgtgtac tcaacagaag
    23281 aatcctgcca atggtcttcg atatcttggt acaaagttcc acccatggca acgttaaaga
    23341 gttgggtacc acggcagaca gagaaaatgg gctttttctg tttaatagct tccttgatga
    23401 gggccagttc gaagatatct ctttgaaggt gatagtcatc actatcaatg gttttgggtt
    23461 cgccataaaa ttttggatcg acattttgcc cacctgtcaa gatgagcttg tcaatcaaac
    23521 tgatatagtg gcaggccatt tcttgatcac caatcggtag gatgatggga atccctccag
    23581 catctttaac gccttcaaca aagccttttg ctgcgtagct catcatgatg tcatcatctg
    23641 gatgagtttt ttcgtttcct gtaatcccaa taactggttt tttcataaaa tgattttcgc
    23701 tttctaatcc tcttttcgca tgaagtagag gagggtttgg agttcacttg tcaaatcgac
    23761 atactgaacg accacgtctt ttggtaaatg cagatggact ggtgaaaaac tgagaattcc
    23821 tttcacacca gcatcaacca agagattagc aacctcttgt gacttgacgc tgggaacagt
    23881 taggatagca gtcttcacat cagcatcctt gattttatcc ttgatctgag aaatcccgta
    23941 aatgggaatc ccgtcaggag tttgggtacc gacttcagga tggtcgtcta ggtcaaaggc
    24001 catgataatc ttcatcttgt tacgttcgtg gaagcggtag tggagaaggg catggcccat
    24061 atttccaata ccaaccagca tgacattggt aatagagttg tcattgagca aatcggcaaa
    24121 aaatgtcatt agttttttga catcatagcc aaaaccacga cgaccaagtt caccaaaata
    24181 ggaaaaatca cgacgtacgg tcgctgaatc aataccgata gcctctgcaa tttgcttaga
    24241 gttggcacgt tcaatctttt ctgcatgaaa tctcttaaaa attcgatagt agagagagag
    24301 tctttttgct gtagcttttg gaatagcaaa ctgtttatct ttcacaaaat cacaaccttt
    24361 ctattcttct attttataga aacattgtga aaaaatcaac aaaaataaga aaaaactaag
    24421 aaaaatctta gttttgatgt aaaaaatctg catgagatag aaaacggtag aggtctccga
    24481 ccagcccctg ataaactttt ttgcccctaa aagtcagaga agtcacataa agtgtatctg
    24541 gtaaggttac acatcctgac aaagtcaaca tgagagcctc atgatcctca tacttgagag
    24601 tacgctctac atgatagcag tccttatagg tcagttcaaa cattttggct ctatctttcc
    24661 gattttgtaa agacaccacg ttctaccaag ctatccatga ggaagtagaa tttttcctga
    24721 tgaatatggt ggtcttctga tttgaaaata tcaactagac gaaggccaaa cttgtcagtg
    24781 atattgattt tagcccctgt aagttccttg ttaatgatga ttttgagttg gaagccttca
    24841 ccgctgtttg gcactttttc caaaaggcga gtcagttcat agttaccaac cttagtttca
    24901 aaaaaggtgt tatctttgag ggtgaatttt ttaacagaag ggctaagagt gtaatcgtaa
    24961 cgacaatttt ttaactgaat gattttttca aatgccatat ggctaacctc cgataatttc
    25021 ttttaaggtt tttgcgaggg tttgtaggtc ttcaacggta ttttgtggcg acaaactgat
    25081 gcgaagggat tccttcaagc gttctgaatt tgcgccatac atggctttaa gaacatggct
    25141 ggattggaca acgcctgcag tacaggctga gccagtagag attgaaattc cagctaaatc
    25201 tagccgaagg agtaagaggt catttttctg accaggaaat ccaatattga gaacataagg
    25261 gagatgatgt tttcctctat tcaagtaata ctgaatgccc tccagctctg ccagaaaggc
    25321 agtttctaga ttttgtacat gttgaaaatg ttcttcttgt ttttctaggt cttcttttag
    25381 ggctgcaacc atgcctacga tggcaggcag attttcagtt cctgcacgtt ttttctgttc
    25441 ctgatctccg ccatgtagat aggaatcaaa gtccatgcta gatgcgtaga gaaaaccgat
    25501 tcccttagga ccatggaatt tgtgggcaga agcagtgaga aaatcaatgc ccaattcttc
    25561 tgaatgaatt gggattttac caatagcctg aactgcatca acatgatagg cagcagggtg
    25621 ttgcttgagt atttggccaa tttcagcgat gggaagtagg tttcctgtct cattattggc
    25681 aaacatagta gaaaccaaaa tcgtatcgtc acgtaaagcc ttttgaattt gctgggctgt
    25741 gatttcttga ttttctggct ggataatggt tgcttcaaac ccaaagtgtt gaaccaagta
    25801 atcaattgtt tcaaggacag catggtgctc gatggcagtt gtgatgatat gttttccttg
    25861 ttcttggtga cgaagacagt agccaatgat ggtagtatta ttgccttcag tcccaccaga
    25921 agtgaaaaag atatgttgag gttttgtcct tagtaactgg gctagttcct gacgggcttc
    25981 tcgcaagagt ttgccagctt gacgaccatg accatgaata ctagaaggat ttccgtgggt
    26041 ttcttgcata accttggtca tagctgaaat agcaactgct gacataggag tcgttgcagc
    26101 attgtccaaa taaatcaaag aatcacctta tttcttttta ttgtaggcaa agagtgggct
    26161 gactggtttt ctttcgtgaa tacggacgat agcatcacca attaactcac tagcagtgat
    26221 gtagcataca tttttaggag ttttttcttt tgttgctact gaatcagtca caagaatttc
    26281 tttaatatta gtattgtcaa gaagctcagc agctccctcg acgaagagac cgtggctaga
    26341 aacagcataa atttctgtag ctccttcacg ttcaacgatt ttagaagctt cagagaaggt
    26401 acgtcctgta tttaaaatat catcaatcaa gatagctttc ttaccttcaa catcaccaat
    26461 aatataacct tcgttacgag ttgcatcgtc ttgagggtag tcgataatgg cgataggagc
    26521 atcaagatat tcagccaggc tacgcgcacg tttgacacct gaatttttag ggctaacgac
    26581 aacaacatct gaaccaagca atcctttatc gcagtaatgt tttgcgaata ggggaacagt
    26641 gaaaagatta tccactggaa tatcaaagaa accttgaacc tgaacggcat gcaaatcaag
    26701 agtcaggata cgatcaactc cagccttaac cagcatattg gcaactagtt ttgctgtaag
    26761 tggctcacga ggacaagcaa tgcggtcttg acgtgcatag ccaaaatatg gaaggacaac
    26821 gttgatactg tgggcacttg cacgcacaca agcatcgacc atgattaaca attccattag
    26881 gtggttgttg acagggaaac ttgttgattg gatgatgtaa acatcataac cacggacact
    26941 ttcttcgata tttacttgga tttctccgtc tgaaaattga cgtgatgata gttttccaag
    27001 tgggacacca acagcttggg caattttttg tgcaatctct tggttagagt tgagtgcgaa
    27061 aagtttcatg ttttttctat ctgacattat agaccgtcct ctgtaaactt tataaatcct
    27121 agttatattt accttacata tatgaactgg gatttgtgta tttttatctt ttctatttta
    27181 ccaaaaaatg gagattattt cagctatttt tcatactttt gacaaatcga accaattttg
    27241 aaggagcttt ttggtaggaa atctgatttt tctctaaaaa ttgtcggaaa tcctgtttgc
    27301 cttgctcatg attttccact tcaagctcca attcgtaatc tgtcatatca aagtatcggc
    27361 tctgatccag tgccatgaga ccaatagctg ttttcatttc atagcgaagc gttgttagac
    27421 aaccaagaac ctgccagttc ttactttgga taccatgttt cgccaattca tccagtacta
    27481 gcccttgagg aagttcttcc ttactcagat agttctcagc atcttttagt tgcaattttt
    27541 ggttgtattc catgtttcca acactctgcg ggactttgag tgtcaactca gcccagtctt
    27601 caaaggttcg aatgcgcata gcgactttct tttctcgcag ttcaaaatca ggcgtgtcga
    27661 tgtagtaatt tgtttgaaga acaggagtga cacctgtgaa ctggtctttt agacgattgt
    27721 attcatcttt tttcaatagt gttttcaatt caatttctaa atgtttcatt tttcttacct
    27781 tttttta
//
LOCUS       DAVKZB010000016        27534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKZB010000016 DAVKZB010000000
VERSION     DAVKZB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.016"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(77..712)
                     /locus_tag="VXA80_000605"
     CDS             complement(77..712)
                     /locus_tag="VXA80_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV0353145.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(714..1541)
                     /locus_tag="VXA80_000606"
     CDS             complement(714..1541)
                     /locus_tag="VXA80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0353146.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1563..1898)
                     /locus_tag="VXA80_000607"
     CDS             complement(1563..1898)
                     /locus_tag="VXA80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV0353147.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYVLEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(1899..2615)
                     /locus_tag="VXA80_000608"
     CDS             complement(1899..2615)
                     /locus_tag="VXA80_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV0353148.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLVHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEEDITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            3138..3749
                     /locus_tag="VXA80_000609"
     CDS             3138..3749
                     /locus_tag="VXA80_000609"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0353149.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3794..4522
                     /gene="rlmB"
                     /locus_tag="VXA80_000610"
     CDS             3794..4522
                     /gene="rlmB"
                     /locus_tag="VXA80_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV0353150.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(4561..5472)
                     /gene="trxB"
                     /locus_tag="VXA80_000611"
     CDS             complement(4561..5472)
                     /gene="trxB"
                     /locus_tag="VXA80_000611"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV0353151.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYEART
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5538..5762)
                     /locus_tag="VXA80_000612"
     CDS             complement(5538..5762)
                     /locus_tag="VXA80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV0353152.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5840..6583)
                     /locus_tag="VXA80_000613"
     CDS             complement(5840..6583)
                     /locus_tag="VXA80_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0353153.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6583..7383)
                     /locus_tag="VXA80_000614"
     CDS             complement(6583..7383)
                     /locus_tag="VXA80_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0353154.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7541..7897)
                     /locus_tag="VXA80_000615"
     CDS             complement(7541..7897)
                     /locus_tag="VXA80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0353155.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7891..8421)
                     /locus_tag="VXA80_000616"
     CDS             complement(7891..8421)
                     /locus_tag="VXA80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV0353156.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGADKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(8421..8915)
                     /locus_tag="VXA80_000617"
     CDS             complement(8421..8915)
                     /locus_tag="VXA80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353157.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            9050..9496
                     /locus_tag="VXA80_000618"
     CDS             9050..9496
                     /locus_tag="VXA80_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV0353158.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            9493..10140
                     /locus_tag="VXA80_000619"
     CDS             9493..10140
                     /locus_tag="VXA80_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV0353159.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10161..10742)
                     /gene="pdxT"
                     /locus_tag="VXA80_000620"
     CDS             complement(10161..10742)
                     /gene="pdxT"
                     /locus_tag="VXA80_000620"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV0353160.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10743..11618)
                     /gene="pdxS"
                     /locus_tag="VXA80_000621"
     CDS             complement(10743..11618)
                     /gene="pdxS"
                     /locus_tag="VXA80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV0353161.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11770..13149)
                     /locus_tag="VXA80_000622"
     CDS             complement(11770..13149)
                     /locus_tag="VXA80_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV0353162.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13443..14366
                     /locus_tag="VXA80_000623"
     CDS             13443..14366
                     /locus_tag="VXA80_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV0353163.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14426..15031
                     /locus_tag="VXA80_000624"
     CDS             14426..15031
                     /locus_tag="VXA80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0353164.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            15050..16294
                     /locus_tag="VXA80_000625"
     CDS             15050..16294
                     /locus_tag="VXA80_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0353165.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16411..16668)
                     /locus_tag="VXA80_000626"
     CDS             complement(16411..16668)
                     /locus_tag="VXA80_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV0353166.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16710..18746)
                     /locus_tag="VXA80_000627"
     CDS             complement(16710..18746)
                     /locus_tag="VXA80_000627"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV0353167.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(18958..19875)
                     /gene="glyQ"
                     /locus_tag="VXA80_000628"
     CDS             complement(18958..19875)
                     /gene="glyQ"
                     /locus_tag="VXA80_000628"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV0353168.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(20244..21005)
                     /locus_tag="VXA80_000629"
     CDS             complement(20244..21005)
                     /locus_tag="VXA80_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0353169.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QPVIWSDFNAATQIIRESAVPTYTNNRRVVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(21278..22120)
                     /locus_tag="VXA80_000630"
     CDS             complement(21278..22120)
                     /locus_tag="VXA80_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV0353170.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(22235..23626)
                     /locus_tag="VXA80_000631"
     CDS             complement(22235..23626)
                     /locus_tag="VXA80_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV0353171.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSSVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(23861..24238)
                     /locus_tag="VXA80_000632"
     CDS             complement(23861..24238)
                     /locus_tag="VXA80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0353172.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSVQDFYTSIDV
                     LAELDNGIQVIIEIQIHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            24471..25448
                     /locus_tag="VXA80_000633"
     CDS             24471..25448
                     /locus_tag="VXA80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0353173.1"
                     /translation="MLKLGIIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            25445..26527
                     /locus_tag="VXA80_000634"
     CDS             25445..26527
                     /locus_tag="VXA80_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0353174.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYISATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDINVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(26591..27534)
                     /locus_tag="VXA80_000635"
                     /pseudo
     CDS             complement(26591..27534)
                     /locus_tag="VXA80_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         8066 a         6228 c         4926 g         8314 t
ORIGIN      
        1 tccgtcactt tttattttaa aggattttag aattttttac aagatattat gctcaaattt
       61 aacattattt agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac
      121 cagcaatact gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt
      181 ctgtcaaaca atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt
      241 gataggcttg attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt
      301 actcctctct ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat
      361 cgcgagcaac ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat
      421 cctttccgat atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata
      481 gatgagcatc aaggttctct agtaacagtc ctgtctgata gccacgaatt cctcgattga
      541 caatcgtctt tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc
      601 caataaaaag aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt
      661 gaattttttc ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct
      721 cctttttcta accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct
      781 agagcagtcg ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca
      841 gccatttcaa gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat
      901 ttcaagtccc agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg
      961 aggatatcaa tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca
     1021 ttgatttctt ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc
     1081 acaccaccaa agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt
     1141 tcaatcattt ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca
     1201 aaatcaccct tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga
     1261 gtcaaagcct tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt
     1321 tcaaaaatac gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt
     1381 agttgtctca tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga
     1441 tccaactgat ctaaaatctt ttccaatcgt gggagatcaa gctgacctct agcatccagc
     1501 aaggtcccat ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca
     1561 agctaatacc acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt
     1621 tccttagtcg cttcatctaa ctggtcacga tgactgccat cctctataaa atcttccaaa
     1681 acataagttt ggtaatgata caagggatat ccttctggtg aaaaggttcc ctttggtgtt
     1741 cgattgaccc aagtaggatg agctttagct gttccgatag ttgtttttcc atccttcttc
     1801 ttgatggtga catccatgag aacaccacgt tcagtccact tagcattctc ttcatctccc
     1861 atagattcaa ttcgttgatt ggaaatgaag ttccccattt aatagacaat gagcttctta
     1921 tctccatctt tttccaccgt ttcagatggt tcaacaacgt gaggatgccc tccaaagata
     1981 atatccgctc cccaatcgat catcttgtga taaagagctt tttgttcttc agttggttcc
     2041 aatcgatact caacacccat ctgaggcatg ataatggtga tatcttcttc cttctctgcc
     2101 cgttcaactt cagcctttat cttatcttcg tttaaatctg aaagataacg attatagtct
     2161 tcctgagaaa tatactgctc aattccattg aaaccatagg aataggctaa caatgcaacc
     2221 ttgatagcat tcacttcctt aatgaccagc ggagcctgat cacgtggttc gtgcgtataa
     2281 actccgattg gagtgattcc agctttctca ataatatcgg ccattgaaat aactccctca
     2341 atttgcgaat ccaaaatatg attatgaact aaatccagca catgataacc tgcctcctta
     2401 atagcatcca taacttcaac aggagcatta aagagaagat aacccgctaa ataatgatcc
     2461 ttattaatgg ttccttcaaa atcaccaata gctaaatctg cttgcttgag ccaaggagtc
     2521 acatactcaa aattttcatg aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa
     2581 agtccatcgt ggtagagcaa atccccgtgg gccataattc tggcagtttt ttcctcttcc
     2641 tactcctgag acttttgctt agtcccctct tgagaaatag tatctcgttt ttgactagtc
     2701 aagggattcc ttggaagctt tcaaacaaca agaccaagcc cattgataaa ccaactgcta
     2761 gcaagagtat cgccacaaat cccttattgc tccacttgcg ataactccta aaaagtttac
     2821 caagcccttc ataaaacgaa aagctaaacc accctgattt cgattttgtc ttctttgtat
     2881 cttcattctc cctactttct tatgcaagcc ttttcttttt attatatcac agataagtat
     2941 ttctttcaca attgaattga acttcccatc tattttctat aaatcctaaa tgccataatg
     3001 ctttcaattc ttgtcatttt gtgatatcat gtagaggtaa tgaaaggaaa tgaactaatc
     3061 cacagtggct tattccaagt ataccacttg ggctttggca gtagttaact gcactaaaca
     3121 taatataagg agagaaaatg tctgcaatag aacgtattac aaaagctgct cacttaattg
     3181 atatgaacga tattatccgt gaagggaatc ctactctacg cgcgattgct gaggaagtca
     3241 ctttccccct atctgaccag gaaatcatcc taggcgaaaa gatgatgcaa ttccttaaac
     3301 attcccaaga tcctgtcatg gctgaaaaaa tgggactccg cggtggtgtt ggactggctg
     3361 ctccccagtt agatatctca aaacgcatta tcgctgtttt ggtacctaat attgttgaag
     3421 aaggcgaaac tccacaggaa gcctacgatt tggaagccat tatgtacaat ccaaaaatcg
     3481 tctctcactc tgttcaagat gctgctcttg gcgaaggaga aggttgcctg tctgttgacc
     3541 gtaacgtgcc tggctatgtt gttcgccatg cccgcgttac tgttgactac tttgacaaag
     3601 atggagaaaa acaccgtatc aaactcaaag gctacaactc cattgttgtt cagcatgaaa
     3661 ttgaccacat taacggtatc atgttttacg atcgcatcaa tgaaaaagac ccatttgcag
     3721 ttaaagatgg tttactgatt cttgaataaa gaaaatcccg ttgcaagacg gggttttgtg
     3781 ttataataga ggcatgaaaa caaatgatat tgtctatggt gtccacgccg ttaccgaagc
     3841 cctccttgca aatacaggaa acaaactcta cctccaagaa gatctccgag gtaagaatgt
     3901 tgagaaagtc aaggaactag ctacagaaaa gaaggtgtcc atttcttgga catcaaaaaa
     3961 atctctctct gagatgactg aaggtgctgt tcatcaaggt tttgttctac gagtgtctga
     4021 atttgcctat agcgagctag attacatcct tgcaaaaaca cgccaagaag aaaatccact
     4081 tctattgatt ctagatggtc taaccgatcc ccataatctg ggttctatct tgcgaacagc
     4141 cgatgcgacc aatgtttcag gtgtcatcat tcccaagcac cgtgctgtcg gagtaactcc
     4201 tgtcgttgcc aaaacagcca caggtgctat tgaacacgtt ccaattgccc gagtgacaaa
     4261 cctcagtcaa accttagata aactcaagga tgaaggcttc tggacctttg gaacggatat
     4321 gaacggtact ccttgccaca agtggaatac aaaagggaaa atcgccctca tcatcggaaa
     4381 tgaaggaaaa ggcatctcta gcaacatcaa aaaacaggtc gatgaaatga ttaccattcc
     4441 gatgaatgga catgttcaaa gcctcaatgc cagtgttgct gcagccattc tcatgtacga
     4501 agtttttcta aatagactat aaaaaagttt ccagtcatct gattggaaac ttttttatga
     4561 ttaactatgt tctgtgataa acttgtaggc ttcttgacca gcgatagctc catctccaac
     4621 tgctgttgtt acttggcgaa ggtctttcaa gcgaacatct ccaactgcaa agataccgtc
     4681 gactgcagtc ttcatgtggc tatctgtcac aatccagcct gcctgatctt ggatattcaa
     4741 ttctttaaca aaatcgctaa gagggtccaa accaacatag ataaagacac caccgaaggc
     4801 ttgctctgtc acttgacctg ttttcacatt ttcaaaaacg acagattcta ctctgttttc
     4861 acccttgatt tccttcacta cagaatccca gataaagctg attttttcat tcgcaaaggc
     4921 gcgatcttgt aaaacctttt gggcacgaag ttggtcacga cggtgaacaa tggtaacagt
     4981 cttagcaaaa cgagtcaaga agagggcttc ttcaacagct gaatctccac caccaactac
     5041 caataaatct tggtcacgga agaaagcacc atcacacaca gcacagtaag aaacaccacg
     5101 actgttcagt tcttcttctc caggcactcc caaaggacgg tgtttagaac cagttgctac
     5161 aataactgta cgtgcttcat atgtttggtc atcagtcatc actttcttaa aatcaccatg
     5221 gtcttcgaca ttttcaacat aaccataaat gtgctcaaca ccaagatttt caagtggttc
     5281 aaacatcttt tcagccaatt caggtccact aatattagcg tatcctgggt aattttcgat
     5341 atcagatgta ttattcatct gaccacctgg cagaccacct tcaatcaaag ctacttttag
     5401 attgcttcga gcagcataca aggccgcagt catccctgca ggtccagcac cgataataat
     5461 agtatcgtac atatagattc cttctttctt gttgtaacta tctttattct aactctttct
     5521 tgtcaatcac gcaaggatta tgccttgaaa acaagaatta aactcataac cgcaaaggat
     5581 aatactggga caaccaagac tccaatcata atcatatcat agagatgggc ttggcgagcc
     5641 ttggctgtct tatcaactcc cgacatggct ctcagaccaa tccaaatccc taaaaaaatc
     5701 agaacaagga tggtggtcaa gatcaaactc tcgaaatata aagaaaatag ttgcagtagc
     5761 atgatttctc tcatttctat cttttttaaa gagtaaactc agctagtcca actaactgag
     5821 ttttccttta tctattatat caaatataag tccgtttgta actagcgaag aattcttttg
     5881 tccgctcttc tttaggggtg tggataatct catccggagt tccagactcg atgattttcc
     5941 ccttatctaa gaagagaatc ttatccgcaa cttgggctac aaaggacatg tcatgactga
     6001 ccaaaatcat ggtctgacct gacttagcag catctgcaat agacttttct acttcaccga
     6061 ccaattctgg gtcaagggct gaagttggtt cgtctaagag caaaacatct ggtttcatag
     6121 caagcgcacg cgctagggca acccgttgct tctgtccacc tgataaatgg cgaggataat
     6181 ggttttcacg gtccgaaagc ccaaccttag ccaactcttc cttggcaatc ttagtcgctt
     6241 cttggtcaga taatttcttg acaacaacca agccttcttt cacattatca agtgctgttc
     6301 ggcgttcaaa caaattaaac tgttggaaaa ccatagacaa cttacggcgt agggcaagga
     6361 tttcttcttg agtgatttta gaaaaatcaa ctgaaaaacc atcaatctga atagagccac
     6421 tgtcaggtgt ttcaagataa ttgagactgc gaagaaaggt tgattttcca gctcctgaag
     6481 aaccaatcaa ggctacaact tccccttttt gaatatccaa gtccagatga tccaagacag
     6541 tctgtcctga aaaggatttg cttaaattcg aaatcttaat cattaacgaa ggtctccttt
     6601 cacatctgtt tgcactgtat caggtgcaga aatagccatt tttctctcga tgaaacgacc
     6661 gaggctttca attccgatat tgactaccca ataaacaagg gcaacggaga tgaagcgttc
     6721 aaaatagcga taatcagctc cacctagaat ctgagcttgg gcaaagactt ccacaacacc
     6781 cgcactaaaa gctagagatg ttcccttggt caaaccgatg agggaattaa tcaaggttgg
     6841 agtagctacc accgctgcat taggaataat cactcgtcga taaacttgcg ctcgggtcat
     6901 acccagactg cgtgccgcct caatctcacc aggattaact gagagaatgg ctgcacgaat
     6961 ggtttcacta gcataagctg cctcattaaa ggcaaaggcg acaatcgcaa aagctgcagc
     7021 tggaatcgca ttgatattga gaccagttcc ccattgctga ttgagggctt tcaaagccaa
     7081 agggattccg tagtaggtca acatgagttg caccaaaatc ggtgtccctt ttaagaaact
     7141 aacaaagaag gcctgcaagg gatataaaat cttgacacga ttgatcttca caatggcaaa
     7201 aagaagcgcc aaaaccaagc caaaaagggc accaccaatt gtcaacataa ttgttgttgg
     7261 aagttgttgg acaattctag ggattccatc aaagaccgaa cgtaggctaa acagcttgcc
     7321 atccggaatc aattgcatca agttttggta ccaatctgat gctaaaatcg ttgtaacatt
     7381 cataaaaaca tcctcttctg ataatgattc attctaccat gcttctgccg tcaatgaaat
     7441 tatttgctac ggtcagatac agactttgtc tacctactag tttatcatac tttgaaacag
     7501 ggaagttata tattttatct atcaaagaga tagataaaaa ctatttcagt cccagttctt
     7561 cataagattt tcgataaccg atttgcttaa cggttccatt ttctactaaa atgggccgtt
     7621 ttaacaacat accgtcactt gctagcaact cagccgcttc ttggtttgac aaacttccta
     7681 ccttatcttt tagccctaat tcacggtatt tgataccact ggtgttgaaa aattgcttca
     7741 attcaaatcc tgaggtttct agccaattca aaatgacttc ttggctaggt gtttcttcca
     7801 cgatatggac ggctttatag tccacaccta attgatttaa ttcttgtttt gcttttttac
     7861 aagttgaaca tttggggtat tcgataaatt ctaacatgtg cttctccttc tattgtttct
     7921 atctttaaaa tctactcctt catgctccaa gagccaagct ttcttttcca ctcctgcagc
     7981 ataacctgtc agacgcttat ctgctcccaa cacacgatga caaggtacta ggatagacca
     8041 aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca ggtcttgagc
     8101 aatttgtcca taggttactg tctgaccata aggaatgccc tgtaaatagt cccaaactct
     8161 cttttcaaaa ttcgttccga ttggcgccaa gagcaagttg gataaatcct gaggcttgcc
     8221 tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac taacaacttc
     8281 ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa cccaaattcc
     8341 atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg agtagagtat
     8401 ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg ttcgccatct
     8461 actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg cataaccttg
     8521 ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat caagccctct
     8581 aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat acggtgaaag
     8641 actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc ataggcctcc
     8701 tctggactct tggtcacagc agcataggct agcagttctc cctcttccaa ggctacgtag
     8761 gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc attttgccac
     8821 tggtcactac cagcatctgc taaacatttt ttggcatcct ccatcacctg catgatggca
     8881 tctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt ctgactagtt
     8941 tctcccatca tagcataatt taagtatttc tacaagcagt taaaacttga ctttgtgttt
     9001 ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa tgaaaactac
     9061 cttttcctac ccaaaatggg cagaaattcc aaacattgac ctctatctgg accaggtttt
     9121 gctctatgtc aatcaggtct gcgcccctat ctctcctaat aaagacaagg gcctaacagc
     9181 atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca aaaaaaaata
     9241 ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg tattttctat
     9301 tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag accaactcta
     9361 cgatgctttt gtggactaca tgaaccaagg aattgaccca gctaacccta ttatccaaac
     9421 cagctgccaa accattaaac tctatcatca aactctagac ttaatcgatc atactcaaga
     9481 ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt tggagaggag
     9541 attgctaata gcgtgaccca tgctgtaggt gcagtcatca tgcttatctt gctgcctatt
     9601 tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt ttccattttc
     9661 gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat ggcctatggt
     9721 tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt tgccattgcc
     9781 ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta tctgattatt
     9841 gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc taaaaaggtc
     9901 aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct ggctatcatt
     9961 cctgccatta tcagtcaaac gacacccgtt ttctggagtc tcatggtaac tggcggactc
    10021 tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca catgatttgg
    10081 catctcttta tcctagctgc gtccgcactt caatacatcg ctattgttta ttacatgtaa
    10141 aaaagttgag aaattcaatc tcaacttttt tctttacaca tattgataaa gtactggtgc
    10201 aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt tttttcttgg
    10261 gctgcaacaa tttgattgtt cactgttgct agaatttcta caccctcacc aacactactg
    10321 ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca ttctgcttcc
    10381 gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat atccatagtt
    10441 cctagatgac tctctttctg agaagtgatt tccttagcca gcaaaattaa gcccgcacag
    10501 gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag catgttctgg
    10561 tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa acccgacaag
    10621 tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc gacacctaat
    10681 tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat tccgattttc
    10741 atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat accaaccatg
    10801 gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa gttagtcaca
    10861 gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat acctgaaccg
    10921 acaaagaccc cctctgcccc taattgcatc attaacgcag catctgctgg cgttgcaaca
    10981 cctccagcag cgaaatttac aactggcaat ttcccatgtt catgaacata ttggaccaat
    11041 tctacaggga cttgcaaatc cttggcagca acataaagct cgtcctcacg taagttttga
    11101 atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac gatatcccct
    11161 gtccctggtt ctcctttggt acgaatcatg gaagcacctt cagcgatacg acgcaaggct
    11221 tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt gtccacatgg
    11281 aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat ttcaatagcc
    11341 tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac tggaatacta
    11401 accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac tcctccagct
    11461 gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc agcagcttct
    11521 gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt gagcatctgt
    11581 gccaagtttt tatttagttc ataacgattt tcagtcattt gttttatcct catcatttgt
    11641 attggtagct accttttcat tttaagtcag aacagaatga agcacaagat aaaaaaacta
    11701 taattgtatg gggacaaatt agctaaaaac tatctgacct taacttaagg ccagatagct
    11761 cattcatttt tatttttcag ccgtaagggc agccattgtg atgtagttgt atggtttgtt
    11821 gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca catgctcttg
    11881 gatagcaagt gagaacatgt ggattcccat gctaattgca atatcatgtg aaaccatttg
    11941 ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta cttcatggtt
    12001 gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag ttgcgttgta
    12061 accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa gaccgtagat
    12121 tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac aagcattgta
    12181 ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc ttgtatcttt
    12241 acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg ggatagatgt
    12301 ttcttgtttc ttgtctacaa ggaaggcacc gttgcgaaag agttcgatct taccatctgc
    12361 aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt caaagctttc
    12421 tttgtcagta atcaagcgtt caactttacc gtcaccttcg attgctttaa cagtttgacc
    12481 tagagccaag cggatgttgt gatcttccaa gttcttcgcc atcatttgtg tgaagtcttt
    12541 gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca agaacaactt cttttccaag
    12601 acgttcaaag gcttcagcaa gttcaacacc gatgtaacca ccaccaacaa cggcgatacg
    12661 gtcgaggtgt tggctcttgt cagaaagttt attgataact tcttcagcat tttggtacaa
    12721 tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt taacaatttc
    12781 aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca atttttcgta
    12841 tgattctttg tgctcttttc cttcaacttc cgctgtaact actttgttat catagtcgat
    12901 tgaaagaaca ggtgagttca tgtaaacttt agcaccttta gcttccaatt tttctttatc
    12961 agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag ccattccaca
    13021 tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct catttccaaa
    13081 attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga ctacaacgat
    13141 tttactcata aaaatttcct gccttcattc taaatttacc ttactagtat accatttacc
    13201 gttagcgttt acaagggggg tactattatt tcagcatttt ttatcaaaaa taattcccca
    13261 tttatcctgt tttcaagcct catgaatttt atgagcatat ttcttgactt tttccaaatt
    13321 ttgtttgtga aaattctaac tttatggtat tataataaca tgaaaatgaa atgtaagggc
    13381 ttcaatttag atgattattt gatattacaa cttattttct ataagagaaa ggagttggta
    13441 gcttgactct tcgttcacat tctgaacggc taatggggac tactatcact atttcattag
    13501 tggatgagca agccgatatc tttctccaaa aatcctttga cttgctcaaa gaacttgaat
    13561 accgcttcaa cgccaatagt caagaatctg agttgatgga aatcaattat caagcaggaa
    13621 tagcccccgt cacggttcat ccagacctgt ttgagttgat ttcacttggg ttagagcata
    13681 gcctagcgct ctctagccac ctcaatatca gcattggtcc cttaattcaa acctggcgta
    13741 tcggtttttc agatgccaag gtcgcccagc ctcaagaaat tgaatcggtg ctgcctctaa
    13801 tcaatcctca tggtatcgag ttagattctt ctacttctac tgtgttttta aaacagaaag
    13861 gaatgaagat cgatctaggt tgtttagcca aaggatacag tgcggataag gttgcccaat
    13921 ttcttaggaa agagggggtg acttctgcct tgatcaatct gggagggaat atcctgacca
    13981 ttggaaaaaa tcaggcaaga ggggataacc catggcaaat cgggattcaa gacccagcca
    14041 atcctagggg aaatcattta atgaccatcc ctgttgtcaa taaatctgtc gtgacttcag
    14101 gcatttatga acgtcacctg accgtcgatg gacaagatta ccatcacatt tttgacagtc
    14161 aaacaggata tcctgttgaa acggaactag cgagtctaac aatcatctct gataaatcag
    14221 tcgatggcga aatctggacg actcgactct ttggagaaag acctgcttct atcctctggc
    14281 aagtcgaaag tttggagggc atcgaagtca tcctcatcga taaagaaggc cacctaagct
    14341 gttcttcagg aattcctact ctatagagac aactgtttca atggaatttt aaaattgtat
    14401 tatgtaaaaa gagaaaggaa atctcatgtt aaaacttatt gctattgttg gaacaaattc
    14461 aaaacgttct acaaaccgtc aattgcttca atacatgcaa aaacacttta ctgacaaagc
    14521 tgaaattgaa cttgttgaaa tcaaggccat tcctgtcttc aacaaaccag ctgacaagca
    14581 agtacctgct gaaatattgg aaattgctgc taaaatcgaa gaggcagatg gcgttattat
    14641 cggtactcct gagtatgatc actctattcc agctgttttg atgagcgctc ttgcttggtt
    14701 gtcttatggt atttacccac ttttgaacaa accaatcatg attacaggtg cttcttacgg
    14761 tacgcttggt tcatctcgtg cccaattgca acttcgtcaa atcttgaatg ctcctgaaat
    14821 caaggcaaat gttcttccag atgaattctt gctctcacac tctcttcaag catttaaccc
    14881 aagtggcgac ttggttgacc ttgatgttat caagaaattg gatgccatct ttgatgactt
    14941 ccgtatcttt gtaaaaatca cagaaaaatt acgtaatgca caagaattac ttcgcaaaga
    15001 tgctgaagac tttgactggg aaaatttgta agataggaga ccaaaaagaa tgaaatttgt
    15061 tggacttgtt ggatcaaact acgatcaatc atataaccgt aagctcttgg aatttatccg
    15121 tcgctacttc aaactcaaat ttgaattaga agttcttgaa attgacgaag ttccaatgtt
    15181 taaccaagat gaaaaatggg atgaaagttt ccaattacgt tatttatata acaaaattac
    15241 tcgtgcagat ggtgttatca ttgctactcc tgagcataac cacactatct cagcttcact
    15301 caaatctgta cttgaatggc tttcatacga agttcatcca tttgaaaaca agcctgttat
    15361 gattgtgggt gcatcatact atgaccaagg aacatcacgt gctcaagttc accttcgtaa
    15421 aatccttgat gctccaggtg ttaatgccta cacgcttcca ggtaacgaat tccttcttgg
    15481 taaagctaag gaagcttttg ataacaacgg aaatatcacc aacgaaggca ctgttaaatt
    15541 ccttgaaact tgcttagata actttgttaa atacgtagga gtcgtttcga aattgaaaaa
    15601 accaaaacca atcgaatcag aagacttgga ttgtggaaaa ccaatcgcta caaccatcac
    15661 agaagttgac cctgacgatc cagaatgggt agaaaaagtt gcagcaatca ctggtgctgt
    15721 ttctggtgat acctatgtca aattggacca cggtatccta acagttaacc aaattgatat
    15781 gttcttgaaa gctatgccat ttgaattgac atacgctgac gacaacaacc aattcctcta
    15841 ctacaacaac gctcaccaag atccagacac tatgttcgct aaacgtgtac cacctcaatc
    15901 aggtagccgt atgtcgactg ttcatggttc tcttccacca gcacgtatga agaacgtaga
    15961 gtgggttatc ggaacacttc gcaacggaaa ccaagaatac gtccgtacga tcgttccagg
    16021 ttctcctgca ggtgttatca acactcacaa ctaccaagca atgtactatc ctgatggatc
    16081 atacgctggt atcaatgaaa tcgtctttaa cttccaacca tggcttgact ggtacctaaa
    16141 agagactggt caacgtttgg tgggtggtag cggaccattt gctcctgctg ccggaggtca
    16201 tggagacgcc gatgctactt ctggcgcttc tgattcaggg gatgctggag gccacggtgg
    16261 tgacgcagac gctacagccg gtgcaagtta ctaatgatag tttttaggaa ccattatggt
    16321 tcctttttta gcgaaaaaag actagcaaac acaaactgct agtctttttg ttatcgtttc
    16381 atctatcttt gttaaaagaa agagtacata ttatgaattt gggtcatcaa gactacggcc
    16441 atgtaatcct ttttcacgtt gtacttggcg tagtttttct ggtgtaacat cgtttccttc
    16501 ttcgtccaca attttgattc cttcaatgtg gtgacgaaca gcgcggcgat aaccttcgat
    16561 gtactcctca cgtagtttgg cttgttccac tttttcttct gatgttaagc cttctgtttt
    16621 tttcttttta gcaagctcat tgatacgagc aatttttttc ggatccattt cttctccttt
    16681 gtaataagat aaagtccgtt tatcaaattt tatttagtgt taatttggtt aaaacaagca
    16741 aacttagctg ctttcttggt tagttgtgac aagattgcca aacgattttg acggacagcc
    16801 tgatcttcag ccattaccat agtattttca aagaaagcat caatgactgg gctaagcgca
    16861 aagagttgtt tcaattgctg acttgcaggt cctgataaaa cgagtgtttc tactgcttct
    16921 gccaaagctt tttcttggtc attctcaaat agtgctgaat caactgtagc aaccccttct
    16981 gccttctcag ccaggttaaa ggcacgagaa agtgattcaa cagatggttt aaaatcttct
    17041 tccttgctta cttctacgag agcacttgct gcttccaaca tatctgccac aacaaagttt
    17101 gaacctgcaa gaactgcttc cttgatatct tttggagtag agcccatcat cttatcaaca
    17161 cgagccttga taaagtccat aacctctgct ttattttcat aagtcaaact gtcaaatttc
    17221 aatgcataaa ggctatcaat cagctcatcc atagcaatgt gccaaccaaa ggcatccaag
    17281 atacgaacca caccttgagt tgcacgacga agggcataag ggtcattaga acctgatgga
    17341 atcaatccta ctgagaagaa actcaaaatc gtatccaatt tgtctgcaat ggctagaact
    17401 gcgccgacct tgctctctgg aagttctcct tcagctgatg taggcatgta gtgttcacga
    17461 atagcagctg ccaccgctgg agtttcacca gcaagaaggg tgtatttttc acccataatt
    17521 ccttggagtt cgtcaaattc accaaccata cctgtcaaca agtcaaactt gtaaatggct
    17581 gctgcacgag caaggtcaac tgtttcatcc actgacaaac cagctttttc tgccaaaagt
    17641 acagtgattt gacccgtacg aatcatgtgt tcacgaaggg aaccaatctt ctcatggaag
    17701 gtgacattgt ttaatttttc aacaagatct gaaatcacca atttttggtc ttcacgccag
    17761 aagaattctc cgtcttccaa gcgggctacc aagacttttt catttccttt gatgacattt
    17821 ttcaaacgct ctgcgtttcc gttacgaaca gaaatgaagt ttggcaagag ttttccatct
    17881 tgatcacgaa caacaaagta acgctggtgt tccttcatag aagtcaccaa gacttcttct
    17941 ggaacttcaa ggtatttagc atcaaaactt cccatgaagg cagttgggta ttcaaccaaa
    18001 ttcaagactt cattcagcaa atccgcatca atttcgatac gtacaccatg ttttgcctca
    18061 atttccttga tttggtcaac aatcatttgc tcacgttcac atggatctgc gattacaaac
    18121 tgcttacgaa ggtcttcttc atagctcaat gctgactgaa ttttggtttc tttccccaag
    18181 aaacgatggc cacggctcac acgacttccc ttgatatcaa ggaaatccaa gtcaaactct
    18241 tgctcatcca agagaacagt taaagtgtga acagggcgga tgtattcaaa gctatttcca
    18301 gcccagtgca tgctgacagg gaaagtcaat gacttcaaga catctacaac accaggaaca
    18361 atggcttcaa ctgcttgacc aacttcttcc ttagtgacat agacatattc ttcacccttg
    18421 atttcacgga attcgatatc ttcaacagtc aaacctttcc cacggacaaa tccttgagct
    18481 gctttggtga agtttccatc actatccaag gcaattttct ttgctggtcc cttgaaatct
    18541 tctgtcaaat cagactgttt gtctgataaa ccagtcacac gaacagccaa acgacgtggt
    18601 gttgagaagg tttgaatggc ttcaaaagac aggcggtttt ccttgaggaa ggctgccatt
    18661 ttttcgccta gttgtttttc acttggtgtg acaacatagg ctggtaattc ttcaagaccg
    18721 agttctacta ataagttttt tgtcatgttt tttctccgaa aagtatcttt ttattttcca
    18781 gtttgcctta tgtgattggc acgcaagcaa ccaacttcgt tgtttcattg ctaaaattgg
    18841 agccaaaagt ctccaatttt gcctagtatc cttagtttca ctaaggatac cttcatcact
    18901 gcggcaactg gctcagggct cactctgttc gcccattttc gtaatttgtc actctcttta
    18961 ttctgcgtct tctgctagga gtttagctct tgtttcttca tccaaaagtg ggtagcctag
    19021 gcgtttgcgt tctgcgacaa aggttttggc tacgacacgg gccaagttac ggatacgagc
    19081 gatatagcct gcacgctctg ttacagatac ggcaccacgc gcgtcaagca gattaaaggt
    19141 atgtgaacat ttgagaacat agtcataggc agggtgtacc aagccttctt ctaatgcacg
    19201 accagcttct ttttcaaact tatcaaagtt ttcaagcaac atttcttggt ccgaaatttc
    19261 aaatgaatat tttgagtgct catactcagg ctggataaag atttctccgt attttacacc
    19321 atcagcccac tcgatatcat agacagagtc tacttcttga atgtaagaag ccaagcgctc
    19381 caaaccatag gtaacttccg cagtcacagg gctagttgcc aatccaccga cttgttggaa
    19441 ataagtgaac tgagtgattt ccattccgtc aagccaaact tcccaaccaa gaccagctga
    19501 accagttgat gggttttccc agttgtcctc aacaaaacga atatcgtgct ccaaaggatt
    19561 gattcccaat ttttccaaag actcaaggta aagttcttgg atatttgatg gagaaggctt
    19621 catgaccacc tggaattggt ggtgttggta gagacggtta gggttttccc cataacgacc
    19681 gtcagcagga cgacgtgatg gctctacata agctgcattc catggctcag gtccgatagc
    19741 acgaaggaaa gtgtaaggac tcattgttcc cgcacctttt tcattatcat aagcctgcat
    19801 aagcatacaa ccttggtcat tccaaaattg ttgcaaagta aggatgattt cctggaaagt
    19861 caatttctta gacattgttt actccttttt atataaatta cttgcgacat gagtctgcct
    19921 cgtcgatcat acgaggcaag acgagttagt actgcgtcta gctcaggcac tctagtgctg
    19981 agcgtggggc agtcctactg taaggaggtt tctgccactg atgcagtaaa atgttaattt
    20041 ctcagacatt gaatactcct taataaaaaa taatttcttg cgacacgagt ctgcctcgtc
    20101 gattatacga ggcaagacga gttagtactg cgtctagctc aggcactcta gtgctgagcg
    20161 tggggcagtc cgactgtaag gaggtttctg ccactgatgc agtgaaatgt taatttctca
    20221 gacattgaat actcctttag tttttatatt tctttttcaa ttaatttctg ctgaacaaac
    20281 caatctaaca agggaaaagt tttttctgta tttgcccata aatgataaga atcaaagacc
    20341 gcttgtacac tacctaaatt ttctaccaac ttatctactg tcaaaaagct gcgccagtat
    20401 tcataaaaaa tacttgcata gttcttatca taagtcgaag aaccaaaatc attcaatgaa
    20461 tgccaaccat acttcttctg aaaaagttct acgagagatt gattacaaat tttttccgct
    20521 tgaaattcct cttctgtcaa gaaatacttg cgactaatat attcaaccat cccctcttcg
    20581 aaccagatat aagagtcata accatcaaaa tcatctaaaa aatgctccga ccaatgagct
    20641 aactcatgtc ctacaatctg taagaggaaa ttttcagata aagaatgata gtggctttct
    20701 attgcttgag tttgctcaga acactcgtag tccatcaact gatacaaata caactctttc
    20761 caaacagcta aatcaggcgt cataaccact cgtctattat ttgtataggt tggaacagca
    20821 ctttccctaa taatctgtgt agcagcatta aaatctgacc aaataacggg ttgcggtaaa
    20881 tcacagactg caagttcatt ctttaaaaaa gattgataat ctttcaactt ttcagtattt
    20941 cttactacaa aatcacgaaa tgtagctagt tgactatcgt cttttacaag ataaaggttg
    21001 tccacggctc tcctttcttt cacacaatag actgaataag caaaagaacc acatctgaca
    21061 atcctaggtt gatttaacct aggggcgtca gaaacgcggt tccaccctaa tttattactt
    21121 cattgttttt gaaaatacta cagaaagcgc cagctcatta ttttgcccta cttggctccc
    21181 actgtcccaa gctcgcttga agaacgccat aagactttct ttcctgctga ctattatatc
    21241 aaaaattaga ataatgtaca attcttttta tgattttcta gaaatccaca tcatcaactc
    21301 gtggagcccc cgactgaaca gcagtcgttt ttaaggtttc tctctcctca tgactcagtt
    21361 caattccaaa acaatcaaga ttagcttgaa tacgagaggt tgtgacagat tttggaagtg
    21421 gtaaaaatcc ttctgccaag ctccaggcca aggctatctg agcaaccgat tttccgtgat
    21481 ttgctgctat ttcttggact tgcttgctat caaacagttc tccttgtcca aaaggccccc
    21541 aagcttccaa taaaattccc ttttcacgac agtaagctac gacttcctct tgatacacac
    21601 ctggcgccaa gcgaacttga ttgaccgcag gaacgatagt tgcagtttca agcaaggcat
    21661 ccaaatgatg gggaagaaaa ttgctaacgc cgatagcacg gattttccct tcttgataga
    21721 ggtcttccat cgctctccaa acttccgcat tgcgagtttt ccatgcgtca ttttctctga
    21781 gcggttttgg gttcggccaa tgaatcaaat acaaatccaa ataatccaag cccagttttt
    21841 ctatagattt ttccaaagct tgacgagttt gctcataggt ttgctgacta ttccaaagct
    21901 tggtagttac gaacatttct tcacgtggaa ctccgctatc tttgattgct tgaccaacac
    21961 tttcttcatt ctgataaatc gccgccgtat caatatgacg ataaccagcc ttcaaggctt
    22021 ctaacactgc acgataggct tcttctccat ccttagcctt aaaagttcca aatcccaata
    22081 ctggaatttc tactccatta tttaattgat aggtattcat atgcttctcc ttctatattt
    22141 attcatcaat tatagcataa aacatttttt aagtagtact atcttctgta aaaaataagt
    22201 ctgtcaaata tctaacagac ctattttttc ttgcttattc atcacgacta tagtacagct
    22261 catgagcttt taggcgagta ttgagcatct ctggtatggt cacaaaggta taaccttgat
    22321 ttttcaaata ctcaatgacc cttggcaagg cattgactgt cggactgtga atatcatgca
    22381 tcaaaacgat agagccgtta gctacttgat gctgaatttc tgtcaaaata gatgcttcat
    22441 ttttactctt ccagtccaga ctatccacat cccacatgat aaagctcaaa tccaagctat
    22501 tgcgaatatc atctgtaata gcaccataag gtggacgcat gagtttagaa ctagaaccca
    22561 gcactttagt tagcacatcc tcagtatcag taatctgctt tttagcttca tcaagagaga
    22621 gttgcgagag aatcggatgg ctccaactat ggtttccaac aacatgacct tcagatttta
    22681 tcctcttcac caagtcctca ttcccagaaa catttttccc aagcacaaag aaagtcgctt
    22741 taatatcata tttagctagg gtctctaata cctgcggggt cgttgctgga tttggaccat
    22801 catcgaaggt tagagcgaca actttttgat atttcttatc aaagtaagat tggtacaagg
    22861 ccgcatcttt ttcgagtaag tacgaagatt ggataacatc aaagaaagca gatactggca
    22921 aggctatctc ttctaaattt tcaaccacag aacttggata aaggataatc tgactatcct
    22981 tgtaatcaaa attccatgca gacaagtctt ggtcagagaa gctttttaca atctgctcac
    23041 tctggtcttg ctctattttt ttatcctcta tgaaggaggt caactctttt atcagctgtt
    23101 ccttagcctt actagcatct gaaaacagtt ggtcaagtgt aaaaggttgc ccatcttctg
    23161 ttaagtgaac ctttcctaga ctggtctttt cagtctcttc aatcttaaaa gcaactaaat
    23221 catagatttg tttggtcaca ttacgattaa cgactccctt taaacctgac tcttcttgct
    23281 ctgtatagta gaaaacaaga ttgtccttac tttctagctt gtccttaaca tcttgattta
    23341 tcagctccct aacagaggaa atcactttct ccccttggag aggataatag gcaatcactt
    23401 cggcttgccc ctgacgaaaa tgctccttct gatttccctc actcaattga tcatctttct
    23461 cttttttgag cgattcaatc ttttgctcaa aacttttttg ctggtagatc ttaaaagcaa
    23521 taaatccgcc taataggcaa atagaaattg ctaaaatacc aatcaaagcc aataatacat
    23581 gtctcgtttt cccgtgtctg ccacgtccca gtctactttt atccatattt atatcataac
    23641 aaatttgagc aataatttca atgatacaat ttggcaatcc acaattcctc tagcaagcca
    23701 aagcatctta catcaactcc aacatccgta tctgtagaga ttatgtagtt ttctatctat
    23761 ggaatttcta gtagattgaa gctagaatag tagacctata cttctaaaac attgttagaa
    23821 atcgatttta ctgtcctgat cgatttgtcc tattctcatt tcattttact ataaaactat
    23881 gaaaagctag gtcatctgag aagtaattac tatcgacaat tgcgatagcg tatactggtg
    23941 cgatttgttt gtagctctgg tgggtatcac cttcacgttg gcgaattttt tctaggtttt
    24001 gattaacctg actgcacaga taagcccata ggcgattgat gaaaaaattc tgatgatgaa
    24061 tctgaatttc gatgataact tggattccat tatctaactc cgccaagacg tctatactag
    24121 tatagaaatc ctgcactgag tagggcatgg aaggcaagac gtgaatgtta cttccctcca
    24181 aaatcgtcac attttttgct ggcaagtcca gcatatcgcg gataaattga caaatcatta
    24241 gtttggctga tacccggatg tctgagaatc ctccttttcc tccttctatt atagcacatt
    24301 tttaaatcta ggtttgctag attctatgct tctatctatt tattcggaaa agaaagcttt
    24361 tcttcagaag aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt
    24421 ctatctatgt tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat
    24481 taggtatcat cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg
    24541 gagaatacca actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt
    24601 ctcgctatca aaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg
    24661 atttagtcta tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt
    24721 ctgctggtaa acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg
    24781 atttgattca aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc
    24841 acgagaaagc ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag
    24901 atttcaacta tgccaaatac tcttccaaga tgcctgactt gttgactgga caaacaccaa
    24961 atgtcttttc agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct
    25021 atgctgctgt tcgtcttttt ggaaaagcca atgacgcgac ttaccatgct caacagcttg
    25081 acaatagcat tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca
    25141 tcaaggctgg aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa
    25201 ccttgactct caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa
    25261 atcaagtcca gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat
    25321 ttgcacacat gatccagcaa ccagatctga atctctacca aacttggctg tatgatgcag
    25381 gctctgttca tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag
    25441 aaaaatgaaa accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca
    25501 agaattcacc cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct
    25561 aggagtgagc caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag
    25621 actacaaaag aaaaaagccc aacaactctt gattctagca ccaaatacgg aacttgctgg
    25681 acagattttt gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt
    25741 cctttcaggt tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct
    25801 gattggaact cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa
    25861 tgtggaaacc atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt
    25921 tgtagaaaaa atcactcact acgcacctcg tgaccaccaa ctggtctaca taagtgccac
    25981 gaccaagttt gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgatca
    26041 aaaattggac aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat
    26101 gctacgaaaa ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc
    26161 agaccttggg aatgccgagg aaaaactaca gtatcgtgat atattggctg tttccctcgc
    26221 tagtgatatt aatgttaaat ttagaaaagt catcttagaa aagtttaaag ataaccaact
    26281 aaccctgctt cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg
    26341 tgtcgtaaac tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac
    26401 tggtcgtatg ggcaaggaag gttatgttat cactctcgtc actcatccag aagaaatcaa
    26461 aaaacttaaa aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat
    26521 caaataaagc cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc
    26581 aattttatac tcatctgctt tcaaaaagca ttctagtcca tctccgatta acgatggact
    26641 ttatcacatc cttctccagt ccttgtatga catcttggag ttgattcatg acatcttcca
    26701 aagttcgaaa ggctttattc ttaaatccac gtttacgaat ctctttcaac acttgttcaa
    26761 tgggttcatc tctggtgtgt atggaggaat aaatgcaaag ccaatattag tcggaatctt
    26821 taaggtactt gatttatgcc atatagcatt gtccataacg agtaaaagat aatcatctgg
    26881 ataagcttgt gaaagctctt ctaaaaaggc gttcatccac tcagtattac atccaccagc
    26941 tattaagaaa aatgattcgc ctgtatgggc atcaacagct ccataacaat agcgaaattc
    27001 tcgtatatag tgactgtgga catgtgaacc tactcctatt ggagaccaac aagatcccag
    27061 tttactgatt ctatcgaaac cagcatcatc ttggtacatc aagcgaacct tatgaaaagg
    27121 tctactatat ttaaaacgct ttcttgtctt cttgaattga gattttattt ttagacgcga
    27181 caatggtttg agcatctgct ttcctaggat gttctggacg tggcgtaata tttcgccaac
    27241 catggcgctt caacagttga tagaaggcat cacgtgtgta ggaacgacct aactcctttt
    27301 tataagcctg aaataaggca tcaattgtaa caaattctcc tgcctctgca gccttcaaat
    27361 gacgggcaag aaaggctttc tcttcctcaa ctgtcatata tgcatggtta cgaccaccac
    27421 gtgtttcttg aaggagagag tcgagtccga actcctcata tttttttaca ttttgctaaa
    27481 tcgttgtttg attacagtct aaaagctcta taatctcttt ataagatttg ccca
//
LOCUS       DAVKZB010000017        27446 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKZB010000017 DAVKZB010000000
VERSION     DAVKZB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.017"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..495
                     /locus_tag="VXA80_000636"
     CDS             <1..495
                     /locus_tag="VXA80_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV0353175.1"
                     /translation="NNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGI
                     GLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVK
                     NLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPA
                     KLES"
     gene            complement(484..1288)
                     /locus_tag="VXA80_000637"
     CDS             complement(join(484..918,920..1288))
                     /locus_tag="VXA80_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV0353176.1"
                     /translation="MNDEASKQLTDARFKRFVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKFHAMKTQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTSRKSSKLKPLTVEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYQNHRKRFGLRMNLIAGIINHELGF"
     gene            1283..1537
                     /locus_tag="VXA80_000638"
     CDS             1283..1537
                     /locus_tag="VXA80_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0353177.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQLKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1782..3116
                     /gene="trmFO"
                     /locus_tag="VXA80_000639"
     CDS             1782..3116
                     /gene="trmFO"
                     /locus_tag="VXA80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV0353178.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3200..3937
                     /locus_tag="VXA80_000640"
     CDS             3200..3937
                     /locus_tag="VXA80_000640"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV0353179.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3946..4503
                     /gene="frr"
                     /locus_tag="VXA80_000641"
     CDS             3946..4503
                     /gene="frr"
                     /locus_tag="VXA80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV0353180.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4563..5417
                     /gene="cvfB"
                     /locus_tag="VXA80_000642"
     CDS             4563..5417
                     /gene="cvfB"
                     /locus_tag="VXA80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV0353181.1"
                     /translation="MNTNLASFILGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5426..5641
                     /locus_tag="VXA80_000643"
     CDS             5426..5641
                     /locus_tag="VXA80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV0353182.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5727..6695
                     /locus_tag="VXA80_000644"
     CDS             5727..6695
                     /locus_tag="VXA80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV0353183.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6673..6852)
                     /locus_tag="VXA80_000645"
     CDS             complement(6673..6852)
                     /locus_tag="VXA80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353184.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6888..7388
                     /locus_tag="VXA80_000646"
     CDS             6888..7388
                     /locus_tag="VXA80_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353185.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            7391..7651
                     /locus_tag="VXA80_000647"
                     /pseudo
     CDS             7391..7651
                     /locus_tag="VXA80_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(8018..8344)
                     /locus_tag="VXA80_000648"
     CDS             complement(8018..8344)
                     /locus_tag="VXA80_000648"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353186.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(8290..8703)
                     /locus_tag="VXA80_000649"
     CDS             complement(8290..8703)
                     /locus_tag="VXA80_000649"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV0353187.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGGGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8704..9129)
                     /locus_tag="VXA80_000650"
     CDS             complement(8704..9129)
                     /locus_tag="VXA80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV0353188.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            9306..9875
                     /locus_tag="VXA80_000651"
     CDS             9306..9875
                     /locus_tag="VXA80_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353189.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9943..10593
                     /locus_tag="VXA80_000652"
     CDS             9943..10593
                     /locus_tag="VXA80_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV0353190.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            10577..12817
                     /locus_tag="VXA80_000653"
     CDS             10577..12817
                     /locus_tag="VXA80_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV0353191.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFEQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKTEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGTIRFKGLDSW
                     KIESVR"
     gene            13216..13803
                     /locus_tag="VXA80_000654"
     CDS             13216..13803
                     /locus_tag="VXA80_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353192.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            13807..14988
                     /locus_tag="VXA80_000655"
     CDS             13807..14988
                     /locus_tag="VXA80_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353193.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVTIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIEKWGSSRKKEVFK
                     V"
     misc_feature    15161..15292
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            15308..15838
                     /gene="infC"
                     /locus_tag="VXA80_000656"
     CDS             15308..15838
                     /gene="infC"
                     /locus_tag="VXA80_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV0353194.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15871..16071
                     /gene="rpmI"
                     /locus_tag="VXA80_000657"
     CDS             15871..16071
                     /gene="rpmI"
                     /locus_tag="VXA80_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV0353195.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            16123..16482
                     /gene="rplT"
                     /locus_tag="VXA80_000658"
     CDS             16123..16482
                     /gene="rplT"
                     /locus_tag="VXA80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV0353196.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16540..16920
                     /locus_tag="VXA80_000659"
     CDS             16540..16920
                     /locus_tag="VXA80_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0353197.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            17170..17970
                     /locus_tag="VXA80_000660"
     CDS             17170..17970
                     /locus_tag="VXA80_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV0353198.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHGRGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            17981..18919
                     /locus_tag="VXA80_000661"
     CDS             17981..18919
                     /locus_tag="VXA80_000661"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV0353199.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPVIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18961..20994
                     /locus_tag="VXA80_000662"
     CDS             18961..20994
                     /locus_tag="VXA80_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV0353200.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(21055..22710)
                     /gene="pavA"
                     /locus_tag="VXA80_000663"
     CDS             complement(21055..22710)
                     /gene="pavA"
                     /locus_tag="VXA80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV0353201.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22815..23312
                     /gene="ybeY"
                     /locus_tag="VXA80_000664"
     CDS             22815..23312
                     /gene="ybeY"
                     /locus_tag="VXA80_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV0353202.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENLELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            23293..23688
                     /locus_tag="VXA80_000665"
     CDS             23293..23688
                     /locus_tag="VXA80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV0353203.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23705..24604
                     /gene="era"
                     /locus_tag="VXA80_000666"
     CDS             23705..24604
                     /gene="era"
                     /locus_tag="VXA80_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV0353204.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24653..25477
                     /gene="mutM"
                     /locus_tag="VXA80_000667"
     CDS             24653..25477
                     /gene="mutM"
                     /locus_tag="VXA80_000667"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV0353205.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTVAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25477..26082
                     /locus_tag="VXA80_000668"
     CDS             25477..26082
                     /locus_tag="VXA80_000668"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV0353206.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            26069..27268
                     /locus_tag="VXA80_000669"
     CDS             26069..27268
                     /locus_tag="VXA80_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV0353207.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            27268..27417
                     /gene="rpmG"
                     /locus_tag="VXA80_000670"
     CDS             27268..27417
                     /gene="rpmG"
                     /locus_tag="VXA80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0353208.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         8101 a         5107 c         6200 g         8038 t
ORIGIN      
        1 aataataact ggatagacaa ggaaggcaga gtatttatct attttactgt cgaagaaatt
       61 atgaaaagaa gaaatatctc aaagccaact gccataaaaa cattagatga gcttgatgta
      121 aaaaaaggaa taggactgat cgaaagagta aggcttggac ttggtaagcc gaacatcatt
      181 tatgttaaag actttatgag tatatttcag gtaaaagaaa atgacttaca gaagtcaaaa
      241 aacttaactt cagaagtaaa agattttaac ctcagaagta aagaaaatga acttcaagag
      301 gttaagaacc ttgactctaa ctatatagag aataataaga gtaagtatag taagagagaa
      361 tatagttttg gtgaaaacgg acttggaaca tttcaaaatg tgtttttagc tgctgaagat
      421 atatcggatt tacaaatcat aatgaactca cagcttgaga attacattag acttcctgca
      481 aaactagaat cctagttcat gattgataat accagcaatc aaattcattc gtaatccgaa
      541 gcgtttacga tgattttgat aggttgttga aaacatttta aacgttttta ctttggcaaa
      601 gatgttctca accttgcttc tctccttaga tagcgcatgg ttacaggctt tatcttcaac
      661 tgttagcggc ttgagtttgc tggatttacg tgaagtttgt gcttgaggat atatcttcat
      721 gagcccttga taaccactgt cagccaagat tttaccagct tgtccgatat ttctgcgact
      781 cattttgaac aacttcatat catgacaata gttcacagtg atatccaaag aaacaattct
      841 cccttgactt gtgacaatcg cttgagtctt catagcgtga aatttctttt taccagaatc
      901 attcgctaat tcttttttta gggcgattga tttttacttc cgtcgcatca atcattaccg
      961 tgtcctcaga gctgagagga gttcttgaaa tcgtaacacc actttgaaca agagttactt
     1021 caacccattg gctccgacgg agtaagttgc tttcgtgaat accaaaatca gccgcaattt
     1081 cttcataagt tcgatattct cgcacatatt gaagagtagc cataagaagg tcttctaggc
     1141 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
     1201 ctaacatctc ttcaaaagtc gtgcgctgaa caccaacaaa acgcttaaat cgtgcatcag
     1261 ttagttgttt acttgcttca tcattcatag aactactata ccatattttg tttcgcagga
     1321 agtctattgg aaagtaagaa atattgaagc tgaggctatt agaagaaatt gtgagcgtgg
     1381 tgctattttt tcaggtaaaa taaaatatca cgaagattca cagcttaaag gagatcacta
     1441 tgttgaatgt tatgctgttt tagataatac ggttatagca agagatagaa taacagtccc
     1501 tatcgatccg ttatgtggaa aagattttat agagtagcat ataattgatt cttaactgga
     1561 atactcacta tctctttaca tcaagaaaat gactaaacag ggaagtttgc cttcttccct
     1621 ttttttgtta tactagtaga agaaaaaata gaaagatttg tgggagtgaa aaagtcctgt
     1681 ggactttttc agcctgagcc aagaaactcg aaagctcgta agtctgattg gctttttcaa
     1741 tgtgaatctt aacttcacac tcccaaagag gtattagtgt cgtgtctcaa tcttatatca
     1801 atgttatcgg tgctggtttg gcaggttctg aagcagctta ccaaatcgca gagcgtggta
     1861 ttccagttaa actttatgaa atgcgtggtg tcaagtcgac tcctcagcac aaaacagaca
     1921 attttgctga gttggtttgt tccaattctt tgcgtgggga tgctttgaca aatgcagttg
     1981 gtcttctcaa ggaagaaatg cgtcgcttgg gttctgttat cttggaatct gctgaggcta
     2041 cacgtgttcc tgccggtgga gcccttgcgg tggatcgtga tggtttctct caaatggtga
     2101 ccgaaaaagt ggtcaaccac cccttgattg aagtggtgcg tgatgaaatt acagaattgc
     2161 caacagatgt tattacagtt gtggcgactg gtcccttgac tagcgatgcc ctggctgaaa
     2221 agattcatgc ccttaataat ggcgatggct tctatttcta cgatgcagca gcgcctatta
     2281 tcgatgtcaa cactatcgat atgagtaagg tctatctcaa atctcgttat gataagggag
     2341 aagcggccta tctcaatgct ccaatgacca agcaagagtt tatggatttc catgaagcct
     2401 tggtcaatgc ggaagaagca ccgctcaatt cttttgaaaa agaaaagtac tttgaaggat
     2461 gtatgccaat cgaagttatg gccaaacgtg gcattaaaac tatgctttat ggccctatga
     2521 agccagtcgg tcttgagtat ccggacgact acacaggacc tcgtgatgga gaatttaaaa
     2581 caccttatgc ggttgtgcaa cttcgtcagg ataatgcagc tggtagccta tacaatattg
     2641 ttggtttcca gacccacctc aaatggggag aacaaaagcg tgtcttccaa atgattccgg
     2701 gtcttgaaaa tgcggagttt gtccgttatg gtgtcatgca tcgcaattct tacatggatt
     2761 caccaaatct tcttgagcag acttaccgtt ccaagaaaca accaaatctc ttctttgctg
     2821 gtcagatgac gggggtggaa ggctatgttg agtcggcggc ttcgggctta gttgcgggaa
     2881 ttaacgcagc tcgtctcttc aaggaagaaa gcgaggttat tttccccgag acgacagcga
     2941 ttggaagctt agctcattac attacccatg ccgacagcaa acatttccaa ccaatgaatg
     3001 tcaattttgg gatcatcaag gagttggaag gcgagcgtat ccgtgataag aaggctcgtt
     3061 atgaaaaaat tgcagagcgt gcccttgccg acttagagga atttttgact gtctaatttt
     3121 tttgaaagaa ttgttcatga tactataaaa atcgtagaaa ttgtgataaa ataggtagga
     3181 tgaaagaagg agagtgaaaa tggcgaatcc caagtataaa cgtattttaa tcaagttatc
     3241 aggtgaagcc cttgccggtg aacgtggcgt agggattgat atccaaacag ttcaaacaat
     3301 cgcaaaagag attcaagaag ttcatagctt aggtatcgaa attgcccttg ttattggtgg
     3361 aggaaatctc tggcgtggag aacctgcagc agaagcaggt atggaccgtg ttcaggcaga
     3421 ttacactgga atgcttggga ctgttatgaa tgctcttgtg atggcagatt cattgcaaca
     3481 agttggggtt gatacgcgtg tacaaacagc tattgctatg caacaagtgg cagagcctta
     3541 tgtccgtgga cgtgcccttc gtcaccttga aaaaggccgt atcgttatct ttggtgctgg
     3601 aattggttca ccatacttct cgacagatac aacagcggcc cttcgcgcag ctgaaatcga
     3661 agcagatgcc atcctcatgg ctaaaaatgg cgtcgatggt gtgtacaatg ccgatcctaa
     3721 gaaggacaag acagccgtta agtttgaaga attgacccac cgtgatgtta tcaacaaagg
     3781 tcttcgtatc atggactcaa cagcctcaac cctctcaatg gacaacgaca ttgacttggt
     3841 tgtcttcaac atgaaccaat caggcaacat caaacgtgtc gtatttggtg aaaatatcgg
     3901 aacaacagtt tcaaataata tcgaagaaaa ggaataagaa agaatatggc taacgtaatt
     3961 attgaaaaag ctaaagagag aatgacccag tctcaccaat cacttgctcg tgaatttggt
     4021 ggtatccgtg ctggtcgtgc caatgcaagc ttgcttgacc gtatacatgt agaatactat
     4081 ggagttgaaa ctcctcttaa ccaaatcgct tcaattacga ttccagaagc gcgtgttttg
     4141 ttggtaacac catttgacaa gtcttcattg aaagacatcg aacgtgcctt gaacgcttct
     4201 gatcttggta tcacaccagc taatgacggt tctgtgattc gcttggttat cccagctctt
     4261 acagaagaaa ctcgtcgtga ccttgctaaa gaagtgaaga aggtcggcga aaatgctaaa
     4321 gtggctgttc gtaatatccg tcgcgatgct atggacgaag ctaagaaaca agaaaaagca
     4381 aaagaaatca ctgaagacga attgaagact cttgaaaaag acattcaaaa agtaacagac
     4441 gatgctgtta aacacatcga cgatatgact gccaacaaag agaaagaact tttggaagtc
     4501 taaaaataaa cagaaaaact cagttggcat tgctggctga gttttattcg aaagaaggaa
     4561 atatgaatac aaatcttgca agttttatcc ttggactgat catcgatgaa aatgaccgtt
     4621 tttactttgt gcaaaaggat ggtcaaacct atgctcttgc taaggaagaa ggtcaacata
     4681 cagtagggga tacggtcaaa ggttttgcat acacggatat gaagcaaaaa ctccgcctga
     4741 caaccttaga agtgactgcc actcaggacc aatttggttg gggacgtgtc acagaggttc
     4801 gtaaggattt gggtgtcttt gtggatacag gccttcctga caaggaaatc gttgtgtcac
     4861 tcgatattct tcctgagctc aaggaacttt ggcctaagaa gggcgaccaa ctctacatcc
     4921 gtcttgaagt ggataagaaa gaccgtatct ggggcctctt ggcttatcaa gaagacttcc
     4981 aacgtcttgc tcgtcctgct tacaacaaca tgcagaacca aaactggcca gccattgttt
     5041 accgtctcaa gctgtcagga acttttgttt acctaccaga aaataatatg cttggtttta
     5101 ttcatcctag cgagcgttac gcagagccac gtttggggca agtattagat gcgcgcgtta
     5161 ttggtttccg tgaagtggac cgcactctga acctctccct caaaccacgc tcctttgaaa
     5221 tgttggaaaa cgatgctcag atgattttga cttatttgga aagcaatggc ggtttcatga
     5281 ccttaaatga caagtcatct ccagacgaca tcaaggcaac ctttggcatt tctaaaggtc
     5341 agttcaagaa agctttaggt ggtcttatga aggctggtaa aatcaagcag gaccagtttg
     5401 ggacagagtt gatttaggga ggcttatgag aaaatcattt tacacttggc tcatgaccga
     5461 gcgcaatcct aaaagtaaca gtcccaaagc aattttggca gacctcgctt ttgaagaggc
     5521 agcttttcca aaacatacag atgattttga tgaggtcagt cgctttttgg aggagcatgc
     5581 cagtttctct tttaacctag gagattttga cagcatttgg caggaatatc tagaacacta
     5641 gcatttattc attgggtttg ggctagtaat ttctccatcc ctctgctata ataaaaagaa
     5701 ataaaaggat tagagaggtt ctttatttga aggaacattc aatagacatt caactgagtc
     5761 atccagatga cctgtttcat ctttttggtt ccaatgagcg ccatcttcgt ttgatggaag
     5821 aagagctgga tgtggtgatt catgctcgta cggagattgt ccaggttttg ggagaagagt
     5881 ctgcttgcga ggaagcccgt caggttattc aagctctcat ggccttggtg aaccgtggga
     5941 tgaccgttgg tacgccagat gtggtgactg caattagcat ggtcaaaaac gatgaaattg
     6001 ataagtttgt cgccctttac gaagaagaaa ttatcaagga taatactggg aaaccgatcc
     6061 gcgttaaaac gttaggtcaa aaactttatg tggacagtgt caaacagcat gatgtgacct
     6121 ttggaattgg gcctgcaggg acggggaaga cctttcttgc agttactttg gcagtgactg
     6181 cccttaaacg tgggcaagtc aagcggatta tcttgacacg tccagcggtg gaagcaggag
     6241 agagtctagg ttttcttccg ggtgatctta aggagaaggt agatccttac cttagacctg
     6301 tttatgatgc cttgtatcag attcttggga aagaccaaac gactcgtctc atggagcgtg
     6361 aaattatcga aattgcgccc cttgcctata tgcgtggccg gaccttggat gatgcctttg
     6421 tcattctcga tgaggcgcaa aacacgacca tcatgcagat gaagatgttc ttgacgcgtt
     6481 taggctttca ttctaagatg attgtcaatg gagatatcag tcagattgac ctgccacgta
     6541 atgtcaagtc cggtttgatt gatgctcaag agaaactcaa gaacatccat cagattgact
     6601 ttgttcattt ttcagccaag gatgtggttc gccatccagt tgtcgctcag attatccgag
     6661 cctatgaata ttctactgaa gttgcacacg actgattttg aggaagttcg cctgcaaaag
     6721 aatatacttt tgagagagga aaatccagtt gtataggcta aaggttttat ccaaaggtct
     6781 gagacaacga ttaggcacga tggaaagaac ttttatgtgg ctgatgacga tcagtgcatc
     6841 ttcctgtgtc ataatcacag ggcacaagaa agtaggaatt tgaaaagatg attgaccaac
     6901 tatctaagta ttacagttgt aggatactaa ctgaaaagga tattccaagt attttatctt
     6961 tatatgaaag taatcctctg tattttcagc attgtccacc agaaccaaat tttgcaactg
     7021 taaaagagga catgctttgt ctacctgaag gtaaagctaa ggctgataag ttttttgttg
     7081 gattttggaa tggatctgac cttgtggctg ttatggattt tgtctatgca tatcctgatg
     7141 aggagactgt ttttattggt ttgtttatgg ttgatcaagc ctatcagagg aaagggattg
     7201 gtagtcatat tgtgacagaa gcactagctt attttgctaa gaactttcga aaggcacgtt
     7261 tggcttatgt taagggaaat ccgcaatctc agcatttttg ggaaaagcag ggctttaaat
     7321 caattggatg cgaggttaag caagaactct atacggttgt tatcgctgaa cagagcctag
     7381 aagattagaa atggcatcaa gtaagaacta tttggaattt gttttggaac aattatcagg
     7441 attagatgat gtgacttacc gttccatgat gggggagtat attctttact tccgcggcaa
     7501 gattattggc ggcatttatg acgatcgctt tttagttaaa cccgtgcaag cagtcttaga
     7561 taagattgac caatcttctt ttgagtttcc atacaaaggt gccaaagaaa tgatttgagt
     7621 ggaagaactt gataataaga tgtttctata agacctgatt ttagctatgt ataaccaact
     7681 gccaacgccc aaacctaaaa agaaaaagca agggtgaacg aagtaaaaaa gaagtctgct
     7741 aaggccctgt ctttgcacgg gtaaaatttt atatataaaa agaagctggg actaaagagc
     7801 tcagcttcct ttggtttata taattgtcat tacaagacga agtggttggg cgaaactctg
     7861 ttgactttat tcaatttaga gtttcttatg cacaattgag tctggaacga aagtctccag
     7921 ttgcaaagta tacagtacaa taaaccaacg atgtaatagc tgatgacaca aagcacagtg
     7981 ggtaggactt gcgaagtcac ccttttcttt tcaaaattta tactaaatca ttgatatcag
     8041 tgtagtcacg attaagtcct tgagcaactg gtaggctagt caagtaacct tgataagtgg
     8101 tcacaccttg acgcaagcct tcatcttcag agattgcttg tgcgaatcct ttgccagcca
     8161 aagcttcgat ataaggaaga gtgacattgg ttagggcgat ggttgaagtg cgggcaaccg
     8221 caccagggat attggcaacg gcatagtgga gaacaccgtg tttttcatag acgggttcat
     8281 cgtgcgttgt cacacggtca gctgtttcga taacgccacc ttggtcaaca gcaacgtcaa
     8341 cgatacagag cctggacgca tttgtttgac catctcatct gtcaccaatt ccggtgcttt
     8401 tgcaccaggg atgagaatgg ctccaatcac cacatcagca tctctcacac ttgcttcaat
     8461 gttgaatgaa ttagacataa gagtttgaat ttgacttcca aagacttctt ctagaactga
     8521 gagacgcttg gcactaatat ctaaaatagt cacttgagca ccaagaccaa gggcgatgcg
     8581 ggcagcatgt gtaccgacga caccaccacc gatgatagtt acttttcctt ttggaacacc
     8641 tggtacacca ccaagtagaa caccagagcc accagcttgc ttagtaagga agtgagctcc
     8701 gatttaaaca gccatacgac ctgcaacctc actcatagga acgaggagcg gtagttgtcc
     8761 ttgattgtca cgaacagttt cataggcaat tcctgttgtt tttgctgcta acatagcatc
     8821 tgctaattct ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat cgtcgcgcaa
     8881 gtaaccgtat tcagaactta aaggttcttt tactttcaca accaactctg ctgcccaagc
     8941 ttcaccagca gtagcgacaa tctcagctcc ttgcttttga tagtcagcat cagtaaagcc
     9001 agaaccgaga ccagcatttg tttcgataag gacacgatga ccacgactaa ctaagctatg
     9061 aacacctgca ggtgtgaggg cgacacggtt ttcgttattt ttaatttctt ttgggattcc
     9121 gattaacatt gagataacct acctttcaat tgacggtctt gttttggttg tcacattcca
     9181 gttcataaat caaaaatgtg acggtttcat tgtatatgaa accgcttcaa aaatcaagaa
     9241 aaacttgtca tcccaatttt tttatgctag actagtgaaa atcaagctct aatggaggga
     9301 aaagtatgga atcaatattt gtgaaatttg cccagtatcc gtctatagaa acggagcgtt
     9361 tattgcttag acctgtaact ttggatgatg cggaagcaat gtttgactat gcctcggaca
     9421 agggtaatac acgttacact tttccaacca atcaaagctt ggaagaaacc aagaataaca
     9481 ttgctcagtt ctacttggct aatcccttgg gacgttgggg aatagaacta aaaagcaatg
     9541 gtcagtttat tggaaccatt gacttacaca agattgatcc tgttcttaag aaggcagcta
     9601 ttggctacat tatcaataaa aagtattgga atcaaggatt aacgacagaa gccaatcgtg
     9661 ctgtgattga gctagctttt gagaagatag ggatgaataa gttgactgcc cttcacgata
     9721 aggctaatcc cgcgtcagga aaggtcatgg agaaatcagg catgcgtttt tcccatgcag
     9781 aaccatatgc ttgtatggac cagcatgaaa aaggccgaat cgtgacaaga gttcattatg
     9841 tcttgaccaa ggaagactat tttgcaaata aataagcagt tgaaaagaaa tttttcgact
     9901 gttttttctt cctcttacga ataatctaag agaggagaaa atatggaagc aattatcgag
     9961 aaaatcaaag agtataaaat catcgtcatc tgtactggtc tgggcttgct tgtaggagga
    10021 tttttcctgc taaaaccagc tccacaaaca cctgtcaaag agacgaattt gcaggctgaa
    10081 gttgcagctg tttccaagga ctcatcgacc gaaaaggaag tgaagaagga agaaaaggaa
    10141 gaaccccttg aacaagatct aatcacagta gatgtcaaag gtgctgtcaa atcgccaggg
    10201 atttatgact tgcctgtagg tagtcgagtc aatgatgctg ttcagaaggc tggtggcttg
    10261 acagagcaag cagacagcaa gtcgctcaat ctagctcaga aagttagtga tgaggctctg
    10321 gtttacgttc ctactaaggg agaagaagca gttagtcaac agactggttc ggggacagct
    10381 tcttcaacaa gcaaggaaaa gaaggtcaat ctcaacaagg ccagtctgga agaactcaag
    10441 caggtcaagg gactgggagg aaaacgagct caggacatta ttgaccatcg tgaggcaaat
    10501 ggcaagttca agtcagtaga cgagctcaag aaggtctctg gcattggtgc caagaccata
    10561 gaaaaactta aagactatgt tacagtggat taagaatttc tctattcccc taatttacct
    10621 gagttttctg ttactatggc tttactacgc cattttctca gtatcctatt ttgctttgtt
    10681 gggttttgtt tttctgctag tctgcctctt tatccaattt ccttggaaat cagcaggtaa
    10741 agttctagtg atttgtggag tctttggctt ctggtttctg tttcaaactt ggcaacagag
    10801 tcaagcgagt caaaatctgg cggattctgt tgaaagggta cggattttgc ctgatactat
    10861 taaggttaat ggtgatagtc tatcctttcg tggcaagtct aacggtcgtg ctttccaagt
    10921 ctattataaa ctccagtccg aggaggagaa agaagccttt caagctttaa ccgacctgca
    10981 tgagatagga ctagaaggga agctttcgga gccagaaggg cagagaaatt ttggtggctt
    11041 taattaccaa gcctatctga agactcaggg aatttaccag actctcaata tcaaaacaat
    11101 ccagtcactt caaaagattg gcagttggga tataggagaa aacttgtcca gtttacgtcg
    11161 aaaggctgtg gtttggatta agacgcactt tccagaccct atgcgcaatt acatgacagg
    11221 actcttgctg ggacatctgg acaccgactt tgaggagatg aatgagcttt attccagtct
    11281 aggaattatc cacctctttg ccctatctgg tatgcaggta ggttttttca tgaatggatt
    11341 taagaaactt ctcttgcgat tgggcttgac ccaagaaaag ttgaaatggc tgacttatcc
    11401 cttttccctt atctatgcgg gactaactgg attttcagca tcggttattc gcagtctctt
    11461 gcaaaagtta ctggctcaac atggggttaa gggcttggat aattttgcct tgacggtgct
    11521 tgtcctcttt attgtcatgc caaacttttt cttgacagca ggaggagtct tgtcctgcgc
    11581 ttatgctttt atcctgacca tgaccagcaa agaaggggag gggctcaagg ctgttactag
    11641 tgaaagtcta gtcatctcct tgggcatatt gcccattcta tccttctatt ttgcggaatt
    11701 tcaaccttgg tctatccttt tgacctttgt cttttccttt ctatttgact tgaccttctt
    11761 accgctcttg tctattttat ttgtcctttc ctttctctat ccagtcattc agctgaactt
    11821 tatctttgaa tggttggagg gcattattcg cttggtgtca caggtgacaa gtagacctct
    11881 ggtctttgaa caacccaatg catggttttt aatcctattg ttaatttcct tggctttggt
    11941 ctatgatttg agaaaaaaca ttaaaaagct aacggtattg tgcttattga ttacagggct
    12001 ctttctcctg accaagcatc cactggaaaa tgaaatcacc atgctggatg tggggcaagg
    12061 agaaagtatt ttcctacggg atgtaactgg gaaaaccatt ctcatagatg taggtggtaa
    12121 ggcagaatct tataagaaaa ccgaaaaatg gcaagaaaag atgacgacca gcaatgcaca
    12181 gagaagcttg ataccctatc ttaaaagtcg aggagtagct aagattgacc agctgatttt
    12241 gacaaatacg gacaaggaac atgtcggaga tttgttagag gtgaccaagg ctttccatgt
    12301 aggggagatt ctagtatcaa aaggcagttt gaagcagaag caatttgtgg tagaactaca
    12361 ggcgactcaa acaaaggtgc gtagtatgat agtaggggag aacttgccca tttttggaag
    12421 tcagttagaa gttctatctc caaggaaaat gggagatgga ggacacgatg ataccctagt
    12481 tctgtatggg aaattcttgg ataagcaatt tctcttcacg ggaaatttgg aggagaaagg
    12541 agagaaggac ttgctgaagc actatccaga cttgaaagta aatgttttga aagctagcca
    12601 acatggcaat aaaaaatcat caagtccagc ctttctagaa aaactcaaac cagagcttac
    12661 tcttatctca gttggaaaga gcaatcgaat gaaactcccc catcaggaaa cattgacacg
    12721 actggaaggt atcaatagca aagtttatcg aactgaccag caaggaacta tacgttttaa
    12781 ggggttggat agttggaaaa tcgaaagtgt tcgataggaa agataaatgt tgtagattag
    12841 tgaaataaac taaaaatttg ttgcataata atgataaaaa tggtataatg aaaacgtatt
    12901 caatattgag gatataaaat cattaaaaat cagcaaaagt tattttatta gttagtttat
    12961 aatctattgg tcttcttcag tccagtgtat ctgctaagag tgttcttaaa agtatcggta
    13021 tgattggaat atcttctata agtctagtat gaatttccat ggatatagac atactaacgt
    13081 tccgtcatgg tctcgttatt acagctattc tgagtatcca gttggttctg gctggaacta
    13141 cggtcgttat gagatcataa actattacag cggaggatat taatagttaa acgtttagaa
    13201 gaaggagagt ttgatatgtt aaggcttact catgttaccc taaaaacgcg acaagtcatc
    13261 ttgcaagatg cggatttcac ctttaaaaag ggtagggttt atggtcttct tgctatcaat
    13321 ggctctggaa agacgaccct gttccgtgcc attagcaatt taattcccat aagtagtgga
    13381 aatatcgcag cccctccttc tttattttat tatgagagcg ttgaatggct ggatggaaac
    13441 ttaagtggga tggactacct tcgtcttatc aaaaacatct ggaagtcagg tctgaacttg
    13501 agggatgaaa tcgcctattg ggaaatgtct gactatatca gtcttcccat tcgcaagtat
    13561 tccttaggca tgaagcaacg cttggtgatt gccatgtatt tcctcagtca ggccaaatgc
    13621 tggctcatgg atgagattac aaatggctta gatgagtatt atcgacagaa gttttttgat
    13681 aggctagcac aaatcgatag acaagaacag ctggttcttt taagttccca ctataaggaa
    13741 gagttggttg atgtctgcga tagagtagta accattcatc aggggcagat agaagaggtt
    13801 tagtttatga aagatgttag tctattttta ttgaaaaaag ttttcaaaag ccgcttaaac
    13861 tggattgtct tagctttatt tgtatctgta ctcggtgtta ccttttattt aaatagtcag
    13921 actgcaaact cacacagctt ggagagcagg ttggaaagtc gcattgcagc caacgagagg
    13981 gctatcaatg aaaatgaaga gaaactctcc caaatgtctg ataccagctc ggaggaatac
    14041 cagtttgcta aaaataattt agacgtgcaa aaaaatcttt tgacgcgaaa gacagaaatt
    14101 ctaactttac taaaagaagg gcgctggaaa gaagcctact atctgcagtg gcaagatgaa
    14161 gagaagaatt atgaatttgt atcaaatgac ccgactgcta gctctggctt aaaaatgggg
    14221 gttgaccgcg aacggaagat ttaccaagcc ctgtatccct tgaacataaa agcacatact
    14281 ttggagtttc cgacccacgg gattgatcag attgtctgga ttttagaggt tatcatccca
    14341 agcttgtttg tggttactat tatttttatg ctaacacaac tatttgcaga aagatatcaa
    14401 aatcatctgg acacagctca cttatatcct gtttcaaaag tgacatttgc aatatcctct
    14461 cttggagttg gagtgggata tgtaactgtg ctgtttatcg gaatctgtgg cttttctttt
    14521 ctagtgggaa gtctgataag tggttttgga cagttagatt atccctaccc aatttatagc
    14581 ttagtgaatc aagaagtaac tattgggaaa atacaagatg tattatttcc tggcttgctc
    14641 ttagctttct tagcctttat cgtcattgtg gaagttgtgt acttgattgc ttactttttc
    14701 aagcaaaaaa tgcctgtcct ctttctttca ctcattggga ttgttggctt attgtttggc
    14761 atccaaacaa ttcagcctct tcaaaggatt gcacatctga ttccctttac ttacttgcgt
    14821 tcagtggaga ttttatctgg aagattatct aagcagattg ataatgtcga tctaaattgg
    14881 agcatgggga tggtcttact tccttgcctg attatccttt tgttagtggg gattctattt
    14941 atcgaaaaat ggggaagttc acgtaaaaag gaagttttta aagtttagtt ttcctatagg
    15001 gaaggtgagg aaaaaactaa cagagtgagg gaaccaacta ggttctctct ttttgattcg
    15061 aaaaccaaac caaaatacaa acacaaacac aaacacaaac ttttcaaaaa ataacttttt
    15121 atcttgacaa gagctagaaa acttggtatc atataaaagt tgagaaaagc agaagtgaga
    15181 gcttctcgcc ttgtgacatt aagttgcctg gccctacgga tgaaaagttt cgaagaaacg
    15241 ctatcataac gtgcgggctt gtatatttac gagtccgcta ttgtttttct ctaataaaac
    15301 aaaagaggtg aaaaccatag caaagcaaga cttattcatc aatgatgaga ttcgtgtacg
    15361 tgaagttcgc ttgattggtc ttgagggaga acagctaggc atcaagccac tcagtgaagc
    15421 gcaagctttg gctgataacg ctaatgttga cctagtattg attcaacccc aagccaaacc
    15481 gcctgttgca aaaattatgg actacggtaa gttcaaattt gagtaccaga agaagcaaaa
    15541 agaacaacgt aaaaaacaaa gcgttgttac tgtgaaagaa gttcgtctaa gtccaactat
    15601 tgacaagggt gactttgata caaaacttcg taatgcacgt aagttccttg aaaaaggaaa
    15661 taaagttaag gtatctattc gctttaaggg tcgtatgatt acccataaag agattggtgc
    15721 aaaagtttta gccgagtttg ctgaagcaac tcaagatatt gccatcatcg aacaacgtgc
    15781 taaaatggat ggacgtcaaa tgttcatgca attggcgcca gcgactgaca aaaaataatt
    15841 gtcagaaagt taaataaagg agaaaaaatc atgccaaaac aaaaaacaca ccgcgcatca
    15901 gctaaacgtt tcaaacgtac aggttctggt ggacttaaac gtttccgtgc ttacacttct
    15961 caccgtttcc acggaaaaac caagaaacaa cgtcgtcatc ttcgtaaagc atctatggtg
    16021 cattcaggag attacaaacg tatcaaagca atgcttactc gcttgaaata atagcttgac
    16081 tgtaagtaaa caattctagg aaatatttgg aggaaataaa atatggcacg tgttaaaggt
    16141 ggcgttgtat cacgcaaacg tcgtaaacgt attcttaaat tagcaaaagg ttactatgga
    16201 gctaaacaca tcttgttccg tactgcaaaa gaacaagtaa tgaactctta ctactatgca
    16261 taccgtgacc gtcgtcagaa aaaacgtgac ttccgcaaat tgtggattac tcgtatcaat
    16321 gcggcagctc gtatgaacgg actttcatac tcacaattga tgcatggttt gaaattggct
    16381 gagatcgaag ttaaccgtaa aatgcttgct gacttggctg ttaacgatgc agtagctttc
    16441 acagctcttg cagatgcagc taaagcaaaa cttggtaaat aacaactgat gagactggaa
    16501 caggattgtc cagtcttttt aaaaataaag gagaaaagta tggcttcaaa aatgctacac
    16561 acttgcttac gagtagaaaa tcttgaaaaa tcaattgcat tctatcaaga tgcttttggt
    16621 tttaaagaat tgcgtcgcag agattttcca gaccatgcct tcacgattgt ctatctaggt
    16681 cttgagggtg atgattatga gttggagttg acttataact acgatcacgg tccgtatgtg
    16741 gttggtgatg ggtttgccca tatcgccctc agtacacctg atcttgaggc acttcatcaa
    16801 gaacacagtg caaaaggcta tgaagtgact gagccaaatg gtctaccagg aactacacca
    16861 aactattact ttgtcaaaga ccctgatgga tataaggtcg aagtcattcg tgaaaaataa
    16921 tttcgtgcaa gttaagtaga atattcgttt tctacttgac tttttttagc tgaaagagta
    16981 tactaataaa aattcaacct ttaaggggag tccagagaga ctcacaaggt gtcagataaa
    17041 agaatagtgc aattttctag aggagacttt ttgagtgtgc tctcttgtgt tgtacgattt
    17101 taactgaggc cttgcactag caaggtcttt tctttatctg gtccccttaa aatttaagga
    17161 ggaaaagtta tgaatcccac atgtaagaag cgtttgggtg tcattcggtt ggaaaccatg
    17221 aaggtggttg cacaagagga aatcgcgcca gcaatctttg aattagttct agaaggagaa
    17281 atggttgaag ccatgcgagc aggccaattt cttcatctgc gtgtaccgga cgatgcccat
    17341 ctcttacgtc gtcctatttc aatttcgtct attgacaagg caaacaagca gtgtcacctc
    17401 atttatcgga ttgagggagc tgggactgca attttttcaa ccttaagtca gggagacact
    17461 cttgatgtga tggggcctca gggaaatggt tttgacttgt ctgaccttga tgagcagaat
    17521 caggttctcc ttgttggtgg tgggattggt gttccaccct tgcttgaggt ggccaaggaa
    17581 ttgcatggac gtggagtgaa agtagtgaca gtcctcggtt ttgctaataa ggatgctgtt
    17641 attttgaaaa cggaattggc tcagtatggt caggtctttg taacgacaga tgatggttct
    17701 tatggcatca agggaaatgt ttccgttgtt atcaatgatt tagatagtca gtttgatgct
    17761 gtttactcgt gtggggctcc aggaatgatg aagtatatca atcaaacctt tgatgatcac
    17821 ccaagagcct atttatctct ggaatctcgt atggcttgtg ggatgggagc ttgctatgcc
    17881 tgtgttctaa aagtaccaga aagcgagacg gtcagccaac gcgtctgtga agatggtcct
    17941 gttttccgca caggaacagt tgtattataa ggagaaaatt atgactacaa atcgattaca
    18001 agtgtctcta cctggtttgg atttgaaaaa tccgattatt ccagcatcag gctgttttgg
    18061 ctttggacaa gagtatgcca agtactatga tttagacctt ttaggttcta ttatgatcaa
    18121 ggcgacaacc cttgaaccac gttttgggaa tccaactcca agagtggcag agacgcctgc
    18181 tggtatgctc aatgcaattg gcttgcaaaa tcctggttta gaggttgttt tggctgaaaa
    18241 gctaccttgg ctggaaagag aatatccaaa tcttcctatt attgccaatg tagctggttt
    18301 ttcaaaacaa gagtatgcag ctgtttctca tgggatttcc aaggcaacta atgtaaaagc
    18361 tatcgagctc aatatttctt gtcccaatgt tgaccactgt aatcatggac ttttgattgg
    18421 tcaagatcca gatttggctt atgatgtggt gaaagcagct gtggaagcct cagaagtgcc
    18481 agtttatgtc aaattaaccc cgagtgtgac cgatatcgtt actgtcgcaa aagctgcaga
    18541 agatgcggga gcaagtggct tgaccatgat caatactctg gttggaatgc gctttgacct
    18601 caaaactaga aaaccaatct tggccaatgg aacaggtgga atgtcaggtc cagcagtctt
    18661 tccagtagcc ctcaaactca tccgccaagt agcccaaaca acagacctgc ctatcattgg
    18721 aatgggggga gtggattcgg ctgaagctgc cctagaaatg tatctggctg gggcatctgc
    18781 tatcggagtt ggaacagcta actttaccaa tccttatgcc tgtcctgtca tcatcgaaaa
    18841 tttaccaaaa gtcatggata aatacggtat tagcagtctg gaagaactcc gtcaggaagt
    18901 aaaagagtct ctgaggtaaa ctgcaatcaa tctgttcttg attttttatt agtttgtaat
    18961 atgaatttag gagaattttg gtacaataaa ataaataaga acagaggaag aaggttaatg
    19021 aagaaagtaa gatttatttt tttagctctg ctatttttct tagctagtcc agagggtgca
    19081 atggctagtg atggtacttg gcaaggaaaa cagtatctga aagaagatgg cagtcaagca
    19141 gcgaatgagt gggtttttga tactcattat caatcttggt tctatataaa agcagatgct
    19201 aactatgctg aaaatgaatg gctaaagcaa ggtgacgact atttttacct caaatctggt
    19261 ggctatatgg ccaaatcaga atgggtagaa gacaagggag ccttttatta tcttgaccaa
    19321 gatggaaaga tgaaacgaaa tgcttgggta ggaacttcct atgttggtgc aacaggtgcc
    19381 aaagtaatag aagactgggt ctatgattct caatacgatg cttggtttta tatcaaagca
    19441 gatggacagc acgcagagaa agaatggctc caaattaaag ggaaggacta ttatttcaaa
    19501 tccggtggtt atctactgac aagtcagtgg attaatcaag cttatgtgaa tgctagtggt
    19561 gccaaagtac agcaaggttg gctttttgac aaacaatacc aatcttggtt ttacatcaaa
    19621 gaaaatggaa actatgctga taaagaatgg attttcgaga atggtcacta ttattatcta
    19681 aaatccggtg gctacatggc agccaatgaa tggatttggg ataaggaatc ttggttttac
    19741 ctcaagtttg atggcaaaat ggctgaaaaa gaatgggtct acgattctca tagtcaagcc
    19801 tggtactact tcaaatccgg tggttacatg acagccaatg aatggatttg ggataaggaa
    19861 tcttggtttt atctcaaatc tgatgggaaa atagctgaaa aagaatgggt ctacgattct
    19921 catagtcaag cttggtacta cttcaaatcc ggtggttaca tgacagccaa tgaatggatt
    19981 tgggataagg aatcttggtt ttacctcaag tctgatggca aaatggctga aaaagaatgg
    20041 gtctacgatt ctcatagtca agcctggtac tacttcaaat ctggtggcta catggcgaaa
    20101 aatgagacag tagatggtta tcagcttgga agcgatggta aatggcttgg aggaaaagct
    20161 acaaataaaa atgctgctta ctatcaagta gtgcctgtca cagccaatgt ttatgattca
    20221 gatggtgaaa agctttccta tatatcgcaa ggtagtgtcg tatggctaga taaggataga
    20281 aaaagtgatg acaagcgctt ggctattact atttctggtt tgtcaggcta tatgaaaaca
    20341 gaagatttac aagcgctaga tgctagtaag gactttatcc cttattatga gagtgatggc
    20401 caccgttttt atcactatgt ggctcagaat gctagtatcc cagtagcttc tcatctttct
    20461 gatatggaag taggcaagaa atattattcg gcagatggcc tgcattttga tggttttaag
    20521 cttgagaatc ccttcctttt caaagattta acagaggcta caaactacag tgctgaagaa
    20581 ttggataagg tatttagttt gctaaacatt aataatagcc ttttggagaa caagggcgct
    20641 acttttaagg aagccgaaga acattaccat atcaatgccc tttatctcct tgcccatagt
    20701 gccctagaaa gtaactgggg aagaagtaaa attgccaaag ataagaataa tttctttggc
    20761 attacagcct atgatacgac cccttacctt tctgccaaga catttgatga tgtggataag
    20821 ggaattttag gtgcaaccaa gtggattaag gaaaattata tcgatagggg aagaactttc
    20881 cttggaaaca aggcttctgg tatgaatgtg gaatatgctt cagaccctta ttggggcgaa
    20941 aaaattgcta gtgtgatgat gaaaatcaat gagaagctag gtggcaaaga ttagtactat
    21001 aagtgaatat gatttgagtg aatagtaagt taaaaatcct gatttcaagt aaaatcagga
    21061 ttttttcatg gatgcaattt ttttggagtc tggtgtgacg cggagggtct tttgtcctgt
    21121 gtaagtgaca aagccgggtt ttccaccagt tggtttattg agttttttga cttcaatcat
    21181 atctacctgc accagattcg acaggcgccc ttgagagaag taggcagcta actctgctgc
    21241 gtctgtcttg actgcatcag atgggtcaag atttcctgag atgacaacat ggcttccagg
    21301 aatgtcctta gcatggaacc aaagttcctc cttgcgggcc attttaaagg tcaattcctc
    21361 attttgaaga ttgtttcgtc cgacatagat gatggttttg ccatcgcttg ctagatattg
    21421 ttctagtttt ttgcgtttct ggattttctc ccgttgtctt ctgcggataa aacctgtttg
    21481 aatcaattct tcacggattt cagcgatttc ttccagtcca gcttggttga ggacggtttc
    21541 tacactttcc agatagagaa tagtggcttt ggtttcttca atcaaatcag tcaagtattt
    21601 gacagcttct ttgagtttct gataccgttt aaaatagcgt tgggcattct ggttgggagt
    21661 cagagcctta tcaagcgcaa tcatgatagg ttggttggta tagtagttgt ctaggataac
    21721 ctggtcttgg tcgttaggca cttggtgtag gaaggttgtc agcaattctc ctttttgacg
    21781 aaattcttca gcgttgtctg tcgccagtaa ctctttttcc tgttttttga gtttgtgtcg
    21841 gtttttctga agttcatttt caacacgacg aatcagttca ctggcctgct gtttgacgcg
    21901 gtcgcgctca gccttatcct tatagtaggt gtccaacaaa tcagaaagat ttgcaaaagg
    21961 ctctcccacc tgatttgcaa aaggaactgg actgaaggaa gtctcagtca agcatggctt
    22021 ggtttcttga ttgaaaaaat ttcggaaagc ggaaagtttt tcactaacca gtatcctttc
    22081 caattcattt gccgtatcgc gtcccagacc ttgaaagagg ctttgaagat tttttgctgt
    22141 tagttcttgg gtttgcagga tttcaaagag cttttcatcc ttgatagtaa aaggattgag
    22201 agattttgta cttggcggag cgatataggt cgatcctgga agtaaggtgc ggtagctatt
    22261 ttgtgaaaag ccgacgtgtt tgataacttc gaggatttta tgactgcttt tatcgaccag
    22321 tagaatatta ctgtgtttcc ccataatttc gataatcaag gtagcctgga tatggtctcc
    22381 aatctcgttt ttattggaaa ctgtaatttc cacaatacgg tcattttcca cttgctcaat
    22441 cgactcaatc agggccccct gcaaatactt tctcaaaacc atgataaagg tagaaggttg
    22501 ggctggattt tcaaaagtcg tttgggttag ctgaatgcgt ccaaaaactg gatgggcaga
    22561 aaggagcagg cgatggcttt ggcgattgct gcggatttgc aagaccaact cttgttcaaa
    22621 aggctgattg attttctgga tgcgaccatt cactaattcg cttcgcaatt cctcaactat
    22681 gtggtgtaaa aaaaatccgt caaatgacat cgttctctcc ttgtgattgt attccatagt
    22741 attatatcaa aaaggtagaa taaaatcatg gaaatgtggt ataataaagc caagtaaaga
    22801 gaaacgagaa gcacatgtat attgaaatgg tagatgaaac tggtcaagtt tcaaaagaaa
    22861 tgttgcaaca aacccaagaa attttggaat ttgcagccca aaaattagga aaagaagaca
    22921 aggagatggc agtcactttt gtgaccaatg agcgtagtca tgaacttaat ctggagtacc
    22981 gtgacaccga ccgtccgaca gatgtcatca gccttgagta taaaccagaa ttggaaattg
    23041 cctttgacga agaggatttg cttgaaaatc tagaattggc agagatgatg tctgagtttg
    23101 atgcctatat tggggaattg ttcatctcta tcgataaggc tcatgagcag gctgaagaat
    23161 atggtcacag ctttgagcgt gagatgggct tcttggcagt acacggcttt ttacatatta
    23221 acggctatga tcactacact ccggaagaag aagcggagat gttcggttta caagaagaaa
    23281 ttttgacagc ctatggactc acaagacaat aaacgaaaat ggaaaaatcg tgacttgata
    23341 tccagtttag aatttgcttt gacaggtatt tttactgcta tcaaggaaga acgcaatatg
    23401 cgaaaacacg cagtgacggc tctagtggtc atccttgcag gttttgtttt tcaggtgtca
    23461 cgaatcgaat ggctctttct cctattgagt attttcttgg tagtagcctt tgagattatc
    23521 aactctgcta ttgaaaatgt ggtggatttg gccagtcact atcacttttc catgctggct
    23581 aaaaatgcca aggatatggc ggccggcgcg gtattagtgg tttctctttt cgcagcctta
    23641 acaggcgcat tgatttttct cccacgaatc tgggatttat tattttaaac agtaagagga
    23701 aattatgact tttaaatcag gctttgtagc cattttagga cgtcccaatg ttgggaagtc
    23761 aaccttttta aatcacgtta tggggcaaaa gattgccatc atgagtgaca aggcgcagac
    23821 aacgcgcaat aaaatcatgg gaatttacac gactgataag gagcaaattg tctttatcga
    23881 cacaccaggg attcacaagc ctaaaacagc tctcggagat ttcatggttg agtctgccta
    23941 cagtaccctt cgcgaagtgg acaccgttct tttcatggtg cctgctgatg aagcgcgtgg
    24001 taagggggac gatatgatta tcgagcgtct caaggctgcc aaggttcctg tgattttggt
    24061 ggtgaataaa atcgataagg tccatccaga ccagctcttg tctcagattg atgacttccg
    24121 taatcaaatg gactttaagg aaattgttcc aatctcagcc cttcagggaa ataacgtgtc
    24181 tcgtctagtg gatattttga gtgaaaatct ggatgaaggt ttccaatatt tcccgtctga
    24241 tcaaatcaca gaccatccag aacgtttctt ggtttcagaa atgattcgcg agaaagtctt
    24301 gcacctaact cgtgaagaga ttccgcattc tgtagcagta gttgttgact ctatgaaacg
    24361 agacgaagag acagacaagg ttcacatccg tgcaaccatc atggtcgagc gcgatagcca
    24421 aaaagggatt atcatcggta aaggtggcgc tatgcttaag aaaatcggta gcatggcccg
    24481 tcgtgatatc gaactcatgc taggagacaa ggtcttccta gaaacctggg tcaaggtcaa
    24541 gaaaaactgg cgcgataaaa agctagattt ggctgacttt ggctataatg aaagagaata
    24601 ctaagtagag gtaggctcat gcctgcttct tgtttttaca gaaggaggac ttatgcctga
    24661 attacctgag gttgaaaccg tttgtcgtgg cttagaaaaa ttgattatag gaaagaagat
    24721 ttcgagtata gaaattcgct accccaagat gattaagacg gatttggaag agtttcaaag
    24781 ggaattgcct agtcagatta tcgagtcaat gggacgtcgt ggaaaatatt tgctttttta
    24841 tctgacagac aaggtcttga tttcccattt gcggatggag ggcaagtatt tttactatcc
    24901 agaccaagga cctgaacgca agcatgccca tgttttcttt cattttgaag atggtggcac
    24961 gcttgtttat gaggatgttc gcaagtttgg aaccatggaa ctcttggtgc ctgacctttt
    25021 agacgcctac tttatttcta aaaaattagg tcctgaacca agcgaacaag actttgattt
    25081 acaggtcttt caagctgccc ttgccaagtc caaaaagcct atcaaatccc atctcctaga
    25141 ccagaccttg gtagctggac ttggcaatat ctatgtggat gaggttctct ggcgagctca
    25201 ggttcatcca gctagacctt cccagacttt gacagcagaa gaagcgactg ccattcatga
    25261 ccagaccgtt gctgttttgg gccaggctgt tgaaaaaggc ggttccacca ttcggactta
    25321 taccaatgcc tttggggaag atggaagcat gcaggacttt catcaggtct atgataagac
    25381 tggtcaagaa tgtgtacgct gtggtaccat cattgagaaa attcaactag gcggacgtgg
    25441 aacccacttt tgtccaaact gtcaaaggag ggactgatgg gaaaaatcat cggaatcact
    25501 gggggaattg cctcaggtaa gtcaactgtg acaaattttc taagacagca aggctttcaa
    25561 gcagtggatg ccgacgcagt cgtccaccaa ctacagaaac ctggtggtcg tctgtttgag
    25621 gctctagtac agcactttgg gcaagaaatc attcttgaaa acggagaact caatcgccct
    25681 ctcctagcta gtctcatctt ttcaaatcct gaagagcaaa aatggtctaa tcaaattcaa
    25741 ggggagatta tccgtgagga actggctact ttgagagaac agttggctca gacagaagag
    25801 attttcttca tggatattcc cctacttttt gagcaggact acagcgattg gtttgctgag
    25861 acttggttgg tctatgtgga ccgagatgcc caagtagaac gcttaatgaa aagggaccag
    25921 ttgtccaaag atgaagctga gtctcgtctg gcagcccagt ggcctttaga aaaaaagaaa
    25981 gatttggcca gccaggttct tgataataat ggcaatcaga accagcttct taatcaagtg
    26041 catatccttc ttgagggagg taggcaagat gacagagatt aactggaagg ataatctgcg
    26101 tattgcctgg tttggtaatt ttctgacagg agccagtatt tctttggttg taccttttat
    26161 gcccatcttc gtggaaaatc taggtgtagg gagtcagcaa gtcgcttttt atgcaggctt
    26221 agcaatttct gtctctgcta tttccgcggc gctcttttct cctatttggg gtattcttgc
    26281 tgacaaatac ggccgaaaac ccatgatgat tcgggcaggt cttgctatga ctatcactat
    26341 gggaggcttg gcctttgtcc caaatatcta ttggttaatc tttcttcgtt tactaaacgg
    26401 tgtatttgca ggttttgttc ctaatgcaac ggcactgata gccagtcagg ttccaaagga
    26461 gaaatcaggc tctgccttag gtactttgtc tacaggcgta gttgcaggta ctctaactgg
    26521 tccctttatt ggtggcttta tcgcagaatt atttggcatt cgtacagttt tcttactggt
    26581 tggtagtttt ctatttttag ctgctatttt gactatttgc tttatcaagg aagattttca
    26641 accagtagcc aaggaaaagg ctattccaac aaaggaatta tttacctcgg ttaaatatcc
    26701 ctatcttttg ctcaatctct ttttaaccag ttttgtcatc caattttcag ctcaatcgat
    26761 cggccctatt ttggctcttt atgtacgcga cttagggcag acagagaatc ttctttttgt
    26821 ctctggtttg attgtgtcca gtatgggctt ttccagtatg atgagtgcag gagtcatggg
    26881 caagctaggt gacaaggtgg gcaatcatcg tctcttggtt gtcgcccagt tttattcagt
    26941 catcatctat ctcctctgtg ccaatgcctc tagccccctt caactaggac tctatcgttt
    27001 cctctttggc ttgggaaccg gtgccttgat tcccggggtt aatgccctac tcagcaaaat
    27061 gactcccaaa gccggcattt cgagggtctt tgccttcaat caggtattct tttatctggg
    27121 aggtgttgtt ggtcccatgg caggttctgc agtagcaggt caatttggct accatgctgt
    27181 cttttatgcg acaagccttt gtgttgcctt tagttgtctc tttaacctga ttcaatttcg
    27241 aacattatta aaagtaaagg aaatctagtg cgagtaaaaa ttaatctcaa atgctcctct
    27301 tgtgacagta tcaattacct aaccagtaaa aattcaaaaa cccatccaga caagattgag
    27361 gttttaaagt attgtcccaa ggaaagaaaa gtaaccctac atcttgaatc taagtagatt
    27421 ttatggtaaa ataataggga ttttaa
//
LOCUS       DAVKZB010000018        26749 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKZB010000018 DAVKZB010000000
VERSION     DAVKZB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.018"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(84..563)
                     /locus_tag="VXA80_000671"
     CDS             complement(84..563)
                     /locus_tag="VXA80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV0353209.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            complement(685..2643)
                     /gene="ftsH"
                     /locus_tag="VXA80_000672"
     CDS             complement(685..2643)
                     /gene="ftsH"
                     /locus_tag="VXA80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV0353210.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2659..3201)
                     /gene="hpt"
                     /locus_tag="VXA80_000673"
     CDS             complement(2659..3201)
                     /gene="hpt"
                     /locus_tag="VXA80_000673"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV0353211.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(3205..4482)
                     /gene="tilS"
                     /locus_tag="VXA80_000674"
     CDS             complement(3205..4482)
                     /gene="tilS"
                     /locus_tag="VXA80_000674"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV0353212.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(4479..5747)
                     /locus_tag="VXA80_000675"
     CDS             complement(4479..5747)
                     /locus_tag="VXA80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV0353213.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSFPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5867..6235)
                     /locus_tag="VXA80_000676"
     CDS             complement(5867..6235)
                     /locus_tag="VXA80_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV0353214.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(6228..6494)
                     /locus_tag="VXA80_000677"
     CDS             complement(6228..6494)
                     /locus_tag="VXA80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV0353215.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6552..10061)
                     /gene="mfd"
                     /locus_tag="VXA80_000678"
     CDS             complement(6552..10061)
                     /gene="mfd"
                     /locus_tag="VXA80_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV0353216.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELVLQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(10062..10631)
                     /locus_tag="VXA80_000679"
     CDS             complement(10062..10631)
                     /locus_tag="VXA80_000679"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV0353217.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10702..11817)
                     /gene="ychF"
                     /locus_tag="VXA80_000680"
     CDS             complement(10702..11817)
                     /gene="ychF"
                     /locus_tag="VXA80_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV0353218.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11937..13103)
                     /locus_tag="VXA80_000681"
     CDS             complement(11937..13103)
                     /locus_tag="VXA80_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0353219.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALEWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(13199..13651)
                     /locus_tag="VXA80_000682"
     CDS             complement(13199..13651)
                     /locus_tag="VXA80_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV0353220.1"
                     /translation="MDKINYFSAEEAILLHDIEVIEKIGGLHGVKDKGRVFSVFEHLQ
                     NDDYYPDFETKLTVLVFNTVQFHMFNDGNKRSSIVFGIKFLTINHLDYLIPFFIETME
                     NIVLWVAMGLIDFNFLKEIISSILYYEELTEKVKEKLVQIALAEENNK"
     gene            complement(13651..14655)
                     /locus_tag="VXA80_000683"
     CDS             complement(13651..14655)
                     /locus_tag="VXA80_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0353221.1"
                     /translation="MTDLTTSQIERQNILNNSLALQKAEEILKVPGFYFEDTFYFTNS
                     QLATFFEVDIRTIERLVEAHKTELTENGYHTLRGEKLAKFKENAFATDTNVGSKVTQL
                     SISSFRTLLNFAMLLTNSDIAKQVRNTLLDIVINVLTEKTGGHVKFINQRDKNYLSTA
                     LEEDNARKIFTNAIDKYVSGSNFKYGQLTNEIYKAIFKERASEYKKILKLNKNDNARA
                     TMYSEVLIVIASFEKGVAYEIEQKSKSSNQKLTNTDVIEIIQRLANHPMQEPHLMHAR
                     EKMASRDLSFRNAKHEKLAEYIRPISEEDFERFIGEQSKSLSEQIDEHRQVFERLRDK
                     "
     gene            complement(14804..15760)
                     /locus_tag="VXA80_000684"
     CDS             complement(14804..15760)
                     /locus_tag="VXA80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0353222.1"
                     /translation="MAKNSPQDLENREYFSKRLNLLMEQKGVRQIDLHNALDIPKSTI
                     TGYVKGKSLPTAGNLQKIADFLNVKKSFLDLRFIDTVSTSTLKELLDNNQREKVFEMF
                     FNKIKEILSFKKDYMNMEYIRGKIAFDQGVLGRVLQSSIINPGIVEIFNGLDLSDISK
                     NNEALLLSKQREAREYAYNNVLTMITNEYITRLINVIEREQALNTSYVHSEISNLINS
                     LNNKIDSYGAVVFEVNEIIEKIGIQDRKILGYCLQRKISSVQNEINDLVHNNTNNSFC
                     VTKNKIFTEKSDIIDTIEFDYYKGIQNRLNDLAEEIREHFSD"
     gene            16086..16733
                     /locus_tag="VXA80_000685"
     CDS             16086..16733
                     /locus_tag="VXA80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV0353223.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIKTWEAKPKHAHSTMNNLLLKAVTDMNAKQLVKSRGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKNTPKKRVRATKKA"
     gene            17165..17617
                     /locus_tag="VXA80_000686"
     CDS             17165..17617
                     /locus_tag="VXA80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353224.1"
                     /translation="MTETTYENLTRRINRISAEMQEIGEKNGLSRLSLLASQTKFIKE
                     ELSRLLWIELPELNDSHKIEAVSKSTTGMFFHPGIFEMDAMRQAFFKRQAMHFFENEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            17614..17814
                     /locus_tag="VXA80_000687"
     CDS             17614..17814
                     /locus_tag="VXA80_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353225.1"
                     /translation="MTLDLDNMTQAEFDKQMAEIKERNPNIFQLISDFLDRKVTPEEV
                     DEFLKMERAEQVEYIKNYQARV"
     gene            17816..18031
                     /locus_tag="VXA80_000688"
     CDS             17816..18031
                     /locus_tag="VXA80_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353226.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPDYGRYIQLAGVNVWGGME"
     gene            18028..18321
                     /locus_tag="VXA80_000689"
     CDS             18028..18321
                     /locus_tag="VXA80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353227.1"
                     /translation="MSYTMNLYPDIEKIPDETYFTKEIFLATCLNNTIRIAVARGVLT
                     SQLLDKETKEVVCKTLDTIIENQKAILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            18302..18643
                     /locus_tag="VXA80_000690"
     CDS             18302..18643
                     /locus_tag="VXA80_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0353228.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            18636..18911
                     /locus_tag="VXA80_000691"
     CDS             18636..18911
                     /locus_tag="VXA80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353229.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            19051..19911
                     /locus_tag="VXA80_000692"
     CDS             19051..19911
                     /locus_tag="VXA80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV0353230.1"
                     /translation="MTIYEARGFQSNLVYPFDKMEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVAVTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            19964..21433
                     /locus_tag="VXA80_000693"
     CDS             19964..21433
                     /locus_tag="VXA80_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV0353231.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLVPVKNGIIDLRTKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFGEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVPSEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            21706..21873
                     /locus_tag="VXA80_000694"
     CDS             21706..21873
                     /locus_tag="VXA80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353232.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEINDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            21956..22486
                     /locus_tag="VXA80_000695"
     CDS             21956..22486
                     /locus_tag="VXA80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353233.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTAMVAGHALLGYMTGTYEVPS
                     ISITHKDKGTLVAEYVEDHKLNKTFKRICDSFKDYYNQPVDDEAFEERYKRERVLQLK
                     ESEDFENLLEKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGDESISLLQKLD
                     VEVEYKGLAGYTEHLK"
     gene            22557..23060
                     /locus_tag="VXA80_000696"
     CDS             22557..23060
                     /locus_tag="VXA80_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353234.1"
                     /translation="MKRLAIETITKPMKLRGISKGIAELDGQRLEIDLDNLMIDFGGE
                     SFELDRIAGTKGGNRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKSTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDSFPERPKYMKRDRYHKHYKKFLKYIEKGDRF
                     WLNGLRL"
     gene            23747..24157
                     /locus_tag="VXA80_000697"
     CDS             23747..24157
                     /locus_tag="VXA80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV0353235.1"
                     /translation="MTPEQVKEKLEGVKWINKEIEGLYLELAALESGIIKKQELSNTR
                     VQTSRVNTTENNPISVLKLKEDTLQRIERLNEERMEISRLIDKLANPLERSVLRLFYL
                     NVLDAWQVAEEIDKSKTAVYIIRQKAIEHLANVG"
     gene            24313..24705
                     /locus_tag="VXA80_000698"
     CDS             24313..24705
                     /locus_tag="VXA80_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0353236.1"
                     /translation="MELDKFKTMMNVRERMTYFLRFQRMAGSENQVTIDEEAWKLVLP
                     YRWDLSGEHEKAIREGLEIFAQDINSIENKRARKYFIIHYCYMRKKTMSECVEMAATS
                     STSYHRYKQIAVLNFARIHQNGELEVYK"
     gene            24759..24917
                     /locus_tag="VXA80_000699"
     CDS             24759..24917
                     /locus_tag="VXA80_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353237.1"
                     /translation="MIEDFLIDLENIQLEDMEIDFNMDIDIDLNIDFEIDVDLDDLDL
                     TLLQELEA"
     gene            complement(25346..25540)
                     /locus_tag="VXA80_000700"
     CDS             complement(25346..25540)
                     /locus_tag="VXA80_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV0353238.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(25605..26741)
                     /gene="dnaN"
                     /locus_tag="VXA80_000701"
     CDS             complement(25605..26741)
                     /gene="dnaN"
                     /locus_tag="VXA80_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV0353239.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTNEDFMQLITPVRTN"
BASE COUNT         8460 a         5027 c         4768 g         8494 t
ORIGIN      
        1 tttcaactct tactattata cagtcttttc aaactttgtc aactactttt tctaattttt
       61 ttttattttt tttgcatgac ttactaatgg gtacggatag taaactcctt aaacacaatg
      121 cgtaagtctc ttaatactct ttgacgccct cgaaatcgtt cattgcttaa gacgcgttct
      181 agttcttctt gtttctcttt actttgtttg tttctataat ctcttagtgt ttcatgaaag
      241 agataataat catctagcca gagacccccc tcacttatac gatgactgat ctcacccact
      301 tcttcatagg gttctttatc gtatctacgc ttctgacttt cctgcttacg gatatagtct
      361 aaaattcgat ttcgaaactt ggttttgaaa tatttcctta aacgtggaat atcgtctgcc
      421 agtccttctt ctctactaat caattcatgt aagcagagca tgccttcttg gtcccaatcc
      481 gacaattccc ataaatgaag gtaatattca tttctacact tatacacagt cccttggact
      541 tcttcataca attctttaat cataattttc ccctttctag atacagtcta acagattaga
      601 aaacacattt gttccatttt ctccattctg ttccctttag ctcctaaact gcacaaaaaa
      661 gagaggctcc agcctctctc agggttattt ttcgtcattc atttttgact ttacttcatc
      721 ataggatagt gcatgagatt cctcttctac tgcttcaggc atctttcctg tttcgtaaag
      781 agctttaatt tgtgtactat ccaatgtttc gtatttcaat aatgcttctg caattaactt
      841 gtgagtttca cgatttgact gaataatttc agcagcttta tttcgtgcct catttaataa
      901 tgaacgaacc tcctcatcaa tttcataagc tgtttgttct gaaattgatt tttgaggact
      961 ctgtgcacca agcatagcat ggtttccttc atattgtact gggccaagtt tttcactcat
     1021 accgtactct gtaaccattg cacgtgccat ttgtgtcgct tgttcaaagt cgtttgaagc
     1081 tcctgtagtt tggacattaa agataatttc ttcagctaca cgtccaccca ttaagccagc
     1141 caattgctct ttcatatctt ctttagatag aagcatttga tcctctttag gaagtgcaat
     1201 catgtatccg cctgcacggc cgcgtggtac aattgtaacc ttatggacaa cgcgagcatt
     1261 cgacaagact agaccaacaa tggtatgtcc tgcctcatgg taagcaacca attctcgttc
     1321 tttttgtgaa actgtcttat ctttcttaga aggtccagca ataactctat cttctgcttc
     1381 atcaatatct gaagcatcaa ttatcgattt attgcgacga gcagcaacta aagctgcttc
     1441 attcaagaca ttctctaaat cagcaccaac aaagcctgga gtttgttgag ccactaattt
     1501 caaatcaaca tcttctgcta aaggcttgtt cttagcgtga actttcaaga ttgcttcacg
     1561 acctttaaca tcaggacggc caaccaatac ttttctatca aaacgtcctg gacgcaaaag
     1621 ggcaggatca agtacatctg aacggtttgt cgcagcgatg acgataatcc cttcatttcc
     1681 ctcaaaacca tccatctcaa tcaaaagttg gttcaaggtt tgttcacgtt cgtcattacc
     1741 tccgccgaga ccgactccac gttgacgtcc aacagcatca atttcatcga taaagatgat
     1801 agctggtgct gcttttttgg catcctcaaa aagagagcga acacgactag ctccaactcc
     1861 gacaaacatt tctacaaagt cagaacctga gatactaaag aatggaacac ctgcttctcc
     1921 agcgactgcc ttagcaagca aagttttacc tgtccccgga ggtccctcca aaagaacacc
     1981 tgctggaata cgggctccaa gttttgtgaa tcgttttgga tcttttaaga actcaacaac
     2041 ttcaactagt tcttgttttt cttcttcagc tccagcaaca tctgaaaatc ttactttaat
     2101 atcttcttta tttgctgctt tagccttact acgtccaaaa ctcattggat tacggccatt
     2161 gcctcctccc atatttccca tcatagagaa taggaagaag aatagaattc caaatggcac
     2221 aatggatacg agtagattaa tccatatacc acttgaactt tcatgcttaa cagttacttc
     2281 tgctttatgg tcagtagcaa gtttttgcaa ttctgatacg gtagtatctg caggaagaat
     2341 agtgctggta aatttctcta ccttagtaac agatggcgtg aaaaactgaa tacctgttcc
     2401 ttctttactt gttttaggat ttttatagac accagaaact tcgataacac taccatttgg
     2461 ttggtaagtt aattctttta cattaccatc ggtaatttct tgtaccaact cagtatagtt
     2521 gatttgctga cttcctcctg agttattccc agaatagaaa tactggaatc ctgtcacaag
     2581 gaaaaagata aataataacc atagaaaagg atttttaatt aaaccattat tttgtttttt
     2641 cattaaagat tattctttct aatttgaata cacttcctct ttcaatactc caatataagg
     2701 aagattacga taattttctt tgtagtctaa accataacct actacaaact catttgggat
     2761 agtaaagcag gtatagtctg cctcaatttc tacaacacgt ccttctggtt tatccaacaa
     2821 ggttgcaatt ttaacagaag ctgcttctct tgctttaaac atatctcgca aattcttcaa
     2881 agtttgacct gtatcaatga tatcttctac aaatagaaca tgtcttcctt tgatatcttg
     2941 agtcacatct tgtttaatat tgataacacc actacttgct gttccaccat ggtagctaga
     3001 aaccatcatg aagtccattt caatatgtgt atcaatatgt ttgaccaatt cagccataaa
     3061 aggaatagat ccttttaaaa tcccaactaa gattggattt tttcctgcat agtctttagt
     3121 taattgagca cctagttttt tagctgcttc tgtaatttca tcgtgtgaaa cgaggacttt
     3181 tttaatatcg ttttctaaca ttttttacct atctattttt tctatataaa gtacagtgtt
     3241 cattatatca tttttcgttt ttttactcaa attattggtc gcaattccca aaattgagac
     3301 aatttcacca aattgctcaa taataagagc agagtttctc ttttccatag ggattttcaa
     3361 atcaataaat aaacgtctga gtttttttct atgcccattt tgaatcaaaa catctcctgt
     3421 ttttcgatga cgaatgtgta tggatgtttc acgtgaaaca ggtatttgtt gaattaattc
     3481 accttctaat ggaagtccaa aagaaaataa atatccttga taagctacct gattttgata
     3541 gtgtaacaca agttcatctt ccttttcatc agcctgcgga ctgattttac aaatctgaaa
     3601 ctgttggtac tcttttatca attcatagcc atttttaatc ggatgacgat actggctttt
     3661 agattttaaa atctgctgaa cttcagcaaa ctgagctttt gtaagattca aatctggaaa
     3721 acgattcaga taagtttgaa gtaaaactct ttgtgtagac tcagagtaag aaaataactg
     3781 ctgtaaattt tccacatcaa tattgttaga taattcagct attgccaaat cataatctaa
     3841 aatttcattg ccgatgccta agattgcatc cctaaatcga ggattttctt tttccaattc
     3901 tggtaagtaa gagtttcgaa tacgatttcg aaaataatga ttctcctgat ttgatgtatc
     3961 ttcaaagtga aaaattgatg gaaagtcttt tttctgaaaa tgcaagaagg gacgaatgat
     4021 ttctatctct ccgactactt gcttctcctt aattcctgat agatagcgca agcgagttcc
     4081 tcgaatcaag cgcatcaaaa tcgtttccac ctgatcatca gcatggtggg cagtgactaa
     4141 agctgtcgca cctgtctttt tcatgacctc ttgaaaaaaa tcataacgaa aatttcgtgc
     4201 acgcgcttct gaaaattctc ctgaaaaatt gctgatataa ataggaagtt ctgcttcagc
     4261 agccaacttc cttaattcct tttcttccca atctgattca attctctgct tatgattcac
     4321 atgagccaga atcaattcaa tctctaactc tttttgataa gtagacaata ccttaaatag
     4381 aaacatagaa tctaatccac cagaaagagc tagaaccgcc ttagcatgct ttttgaaata
     4441 tcccttcttg agaaaatgat ttaaaaaatc ttgttccctc attttagaac ctcataaaca
     4501 tccttggcta tcttagaaat cgtatcataa tcagaattct tagtgaaaat agaaagaata
     4561 aatggagaat ctgcatagac aacacccgta tcatgcttaa attcatccgc atctccaatt
     4621 ttatgagcta ctttaacaga aacacctttg gcaattcgct gactatcaaa atctgtttta
     4681 gtcaaagact ctagcacaaa tccattttga ttataaatag cttccataaa cttcccggcc
     4741 atcttagaag aaatcaattt ttcttttgga tcccaatcat ctcccataat ggcagacatt
     4801 ttggatttga atgtggcatc agattggttt gaaatgtaat atcccaatag attatgagct
     4861 acattatcag attcttttga tactttcgta attaaatcct ttaaagaata ttctttatta
     4921 tcttcttttt taggaagact accacttccc tctggtttat aagaacctgg aaaatcattg
     4981 actgcagata cgtattttac agtcgtatct aactgataaa gaccctcatt tattttttct
     5041 tgcgtataat agagatagga gagtttcaaa acgctggctg catacatctt ttcatcttga
     5101 ttgataccag cttcttttcc agtagtcagt tgcttaacat aaatagagaa agaatctttc
     5161 tgatattttt cagataacat ttcttgaact ttactcatcc gattatcttc ttcagaagtt
     5221 gattctttag ctacccatcc agcctgatca atatgtagaa attctcttcc ttctacaaac
     5281 atggtcttat cgattgatac ttgcgaataa gctgataagg atgatttcac ttcttttaaa
     5341 tcataaggac tattgtacag tttaaagtca gattctaacc atactttttt tattgttgga
     5401 gttacctctg attgatcata taaaaatcgt ttgtccgcag ctataaattg atgatttgat
     5461 agcttaaata ctggaattcc ttgtttattt aagcgccact cggttatttg aaaacttgtt
     5521 ttgggagtca atttcccaga ttccactact aaatcttcat tcgcataaac aggaacctct
     5581 ccataaacca tgggaaaact taatttttct ctaaaataaa taccaaagtc agattgtgaa
     5641 aggtaataaa tttctttcga agtatagacg acttcttttt ctgtgctaac aacttttgaa
     5701 atggtcaaaa aacttggtag caacaaaata attaagaatt tacgcattct tcctttcctt
     5761 ttcttcttgt aaacgcaata attgattttt atcagccaat tcttccagct tttcatctga
     5821 caaactcaaa aattgctcta ttacgtctaa taaattttcc attttatcac ctttgaagca
     5881 agtcaggaat cgtataaact ttttccctcg acttagaata atagtacttc gctcgtgtat
     5941 atttggcagc ataatcttca tctttcaact tggtagcaaa tgctgtttcc ttatcctttt
     6001 catcactcaa agtttgatac tgagtttgca agtctgctaa ttgctgacgt ctttggagta
     6061 attgctgata actctgcgct aaattaaaag ttggcaagat aaataatagc ataatcaaaa
     6121 tcaatacccc ccccataaaa cgattccgtt tttgtcgttc tttcatcagg tagcgacgac
     6181 gttggtattc attttgaata aaagaattat tcaattgtac aatattttta gacattttct
     6241 tctacccgtg tttcactgat aatttcatac atgcctgctg catcttcttt ttttgtacta
     6301 tctttcatct ctagtacttt tacaagcagc aacttattgc caaagcgaat ttcaacttgg
     6361 tcattaactt tcaagtccgt tgaacttttg gccaagattc cattaacctt gattctacct
     6421 ttatctgcta cttcctttgc gactgtacga cgcttgataa ttcgcgatac ttttaaatat
     6481 ttatctaatc tcatccttat tacctcaatt tattattgta ccatttttat ctattttata
     6541 gaagaaaaat atcaaatgga attttcttcc ttaaactctt ttatctctaa taaactttct
     6601 ccaaaaatca gcagaccttc taaaatttca taatctttct tattttggac atcaaataca
     6661 agctccatta atcccttatt ctcagcgatg cctgctttta agttcgttac ggataaagct
     6721 ttaaaataat cttgagctaa aaacagtcgt tgagtgactt tttcaaattg aattgtaatt
     6781 ttattatctt ttctttccac acgttgaaca aagaccttgt ccaagtatga tttaaccaaa
     6841 ccaatctcta aaagataggc tactacatct gggtattctc caaaacggtc tatcaactcc
     6901 tcttgtaact cttcataatt gacacggttg tcaatttgac gaattttctt gtaaatttca
     6961 atcttatgtc gttgatcaga aatataagta tcaggaagat aggcatcaat ttgcaaaacc
     7021 aactcagcat tccctttggt tcttgtgtta gcgttagcat taccgtttcg tttagcaata
     7081 gcttcctcta ataactgcga atacaattca aaaccaacag aatcaatgaa accagactgg
     7141 gattttccta aaagatttcc tgctccacga atcgaaagat ctcgcattgc aatcttaaag
     7201 ccagagccca attctgtaaa tcctttaatc gcttctaatc tcttttcaga gacttcactg
     7261 attgattttt ctggacgata catgagataa gcataagcaa tacgattact acgaccgact
     7321 cttcctctta actgatataa ggttgacaag cccatatggt ccgcattttc aataaataaa
     7381 gtattagcat ttggaatgtc cacccctgtc tcaataatag tagtcgtcac caagatatcg
     7441 tattgtccct caataaagtc taatagagta ttttccaact ggacttcact cattcgacca
     7501 tgaacatatc caatcgaagc ctccggaatc aactcctgta attctgaaac cttctgaaca
     7561 attgtatcaa ctttgttgta aagatagtaa acttgacctc cacgctccat ttcacgcaag
     7621 acagcatcac gaatgacact atcattcttt tccaaaacat aggtctgaac aggatagcga
     7681 ttagtcggcg gagtttcaat aacagacaaa tctctgattc ccagcataga catatggagg
     7741 gtacgaggga ttggcgtagc ggtcaaggtt aggacatcca cttgtttctt cagttctttc
     7801 aaagtttcct tatgcttgac accaaatcgc tgttcctcat caataatcat caagcccaaa
     7861 tcagcaaaca caacatcttt tgacaaaaca cgatgtgttc caatcaaaat atcgacttga
     7921 ccgtttttca atttttcaag tgttgcagtc tgctcttttt tacttctaaa gcgactcaac
     7981 acatcaacat taactgcaaa attttggaat cgttccttaa aattcgtata gtgctgttgc
     8041 gctaaaaccg tcgtcggaac tagaatgaca acctgtttgt gatcattgac tgctttaaag
     8101 gctgcacgca tagcaacttc agtctttcca aaaccaacat ccccaactaa aagtcgatcc
     8161 attggctgag aagcctgcat atccctcttg atttcctcaa tactacgaag ttgatcatcc
     8221 gtttcaacat aagggaaagc atcatcaaag gcatcttgat catcatcatc agctgagaaa
     8281 gcaaaaccct tcaactgact acgttcagag tagagtttga ttaaatcatc agctatatcc
     8341 tctacctggt tcttaacctt ttgcttggcc tttttaaaat gaccgtcatt taatttattg
     8401 agttttggag ctttaccatc acttgaaata tatttggaca gtagatgaat ctgttccacg
     8461 gggatagaaa tttgatcacc attttggtat tggacactga cataatcgcg atgaattccc
     8521 ttgatttcaa tggtttcaat tcctagatat tgaccaatcc catggatatg atggacaaca
     8581 tagtcccctt tttcaagttc attgtaatct tttaatctct ctgcatttga aacatgttgt
     8641 cttcgaaaac gacgctttaa tttcttttga aaaatctcat gttcagttat caataaaatc
     8701 ttttcatcta caaaatgaaa accatgtctg agattaccct ctattaagtt tacagattct
     8761 ttacagatac ttgtcttatc tctagaatcc aatttaatct gatattcctc taacatatcc
     8821 tccaatgttt tacttcccat tgaattgcta gactgcagaa taatggtgta atccattttt
     8881 ttatatcgtt caatttcttc ttttagaaaa gaaaactgat tgaaaaattc ctgcatagga
     8941 tattgattga attgataaat tttgtcaaat ttgagatttc ctaaaccctt ttgaagatta
     9001 gagaaaaagg tcactggact ttgtttttta tagatttgtt caatatcaga aaaatactgc
     9061 atatcagaaa atgctttact attctgtaat tcttctgtaa agtactgcgc taagtctctt
     9121 tcaaagactt catactgatt catcaatttt tgataatcat caaagaatat tggagtatct
     9181 ttttcaatat aatcaaagac agtccatgtc ttatcatagc acaaagataa aaacttccga
     9241 gagtctgcat gactttgttt ttggtgaaaa cttgaaagaa tttcttctag gtatgatttc
     9301 aaaataggtg ataaagtttt tgaaatttgt ttttctaaag ctgactgtcc tcgttgataa
     9361 tccttttctc tcaaaagcat atcactagct ggaaagatag tgagttctgt cttattttct
     9421 ttcgataatt gtgtttctac ttcaaatgac ctgataccat caatttcatc accaaaaaac
     9481 tcaattcgac aaggttctaa ctgggatatt tcaaaaatat ctaaaatatc tcctcgaaga
     9541 ctaaattcgc cctgagtttg tacttgagta acttttcgat agccattttc ctttaactga
     9601 tggataaacg cgtgttgatc atattcttca ccaactgaga tttttacaat actatctttg
     9661 aatgcattgg gagacggtaa aatcaatcga cttgctgcga tattacaaac taaaatccct
     9721 ttcttagatg aatcagtcaa aaaacgcaag gcttcaaccc gtgaaataat tttttcctgt
     9781 gaagacatca aaaactccac cataggagca tcatctacca aaaatggata gacgagttcc
     9841 tcacccaaga tagaaataag atcactaaca agtccttctg cttctccata agttgacgtc
     9901 aataacacaa tcctatcttc tttttctaaa ctgcttgcaa ttgcaagagc cttagtagat
     9961 gttgataaac caagtattag ttgtcttttc ttatctgtta aattttgatg ccatttttta
    10021 atctgatcat tttctgagaa taaatctaat aaggtcacca tttatccgtt atacctctgc
    10081 attgttttct caaaattttt ctcttgtaaa tagtatttta cagaatcgtc aactttgtca
    10141 acagactgta aaataccgat ataatcatcc ctgtcaaact tactcaaaac atgatgaaca
    10201 actgacatac catttttagg tcttccaatt ccaatcttaa cacggttaaa gacctgagtt
    10261 cctatatgtt gaataataga cttgatacca ttatgaccac ctgctgagcc ttttgctctt
    10321 aaacgaattt tcccaacttc catgtcaaga tcatcgtaaa tgataagtaa atcgtcaata
    10381 tccaaaccat agtaagttaa taaagcatga actgcttttc cactttcatt cataaaggtc
    10441 gttggtttaa ccagataaat tttttctcca tttaggaaaa aggatgctag gtcagcttga
    10501 aatatcttat cgtgtgtaaa agtgacattc tgtttcttcg ctagttgatc aatcaacata
    10561 aaaccaacat tgtgttttgt ttcaaaatat ttatcccctg gatttcccaa gcctacaagt
    10621 aatttggtca tttatttttc ctttcaaaag ccaaaagggt tggaattttt ttccaaccta
    10681 attgacacca tttattaatt tttagacatt aaagcggaat tccatgatat cgccatcttg
    10741 aacgatatat tcttttcctt cttcacgcaa gcgtccagct tcttttacgg ccttttcaga
    10801 tccgtatttc actagatctt catatgacat ggttactgca cgaataaagc ctttttcaaa
    10861 gtctgagtgg ataataccag ctgcttgagg agccttcata ccacgtttga aagtccaagc
    10921 gcgaacttct ttttcaccag ctgtgaagta agttcccaat ccaagcaagt ggtaagctgc
    10981 acgcgtcaac ttatctacac ctgattctgt caaaccaatg gcttcaagaa actctttttt
    11041 atcttcatca tccaattcag aaatttcttc ctcagcacgc gcagaaataa cgactacttc
    11101 agcattttct gtcgctgcaa attcacgaat ttgtttgaca tagtcgatag agtcaggttc
    11161 tgaaaccaca tcctcgtcca cattagctac ataaagaact ggtttagtcg tcaaaaggaa
    11221 aagacctttg acaacctttt gttcctcatc tgtaaattca atggtacgag ctgatttccc
    11281 gtcttctagg actggtttaa tcttttgaag aacattgaat tctgctactg attctttatc
    11341 tttttgcgta cgtgccatct tttctacacg cgcatatcgt ttgttcactg attctaagtc
    11401 agcaagaatt aattccagat taattgtatc aatatctgca agtggatcta caaaggcgtc
    11461 ttcacgtcct tgctcgcgca tcacattttc atcatcaaaa gcacgaacta cgtgaacaat
    11521 cgcatctact tcacgaatat tggccaagaa tttattccct agcccctctc cttttgaagc
    11581 tccttttaca atccctgcaa tatctgtaaa ttcaaatgtt gtgggaactg tctttttagg
    11641 agttatcatt tcagttagtt tttgtaggcg ttcatctgga acttccacca ttccaacatt
    11701 tggatcaatc gtcgcaaatg ggtagtttgc tgcctctgct cctgcttttg taattgcatt
    11761 aaatagtgtt gatttaccaa cgtttggcaa accaacgata cctgctgtca aagccatatt
    11821 tctcattctc cgttttcatt tcaatcccta atattataac acaaaaaggg taaacttgct
    11881 aaaattcctt gatatagcta tttcccataa ctgaaataat aattttatgg acttttttag
    11941 gaacttttta atttttcaat agctttttca taaaatgaag tagcattttt ctgattgtct
    12001 ttggataggt ggctataaat atccatggtc atagaaagtt ttgcatgacc gagccgtgtt
    12061 tgtatctctt tgtaaggtag tccagcattg agcaagatac tagcatgagt gtggcggaag
    12121 gcgtggaacg taaaacgagg gcatccagca cgttccaaat gttttactaa cctgaaacta
    12181 agcattctag cgtctatatg cttatcaaag ctattagaga atactttctc ataggtcaac
    12241 cccatttctt tccctatttg ggattgtctt gctttataga gccgtagcat gagcaccgtc
    12301 ttattatcca agtcaattac cctaatactt gatttagtct tagggccagt gatttctttg
    12361 ataatattta acgttttatt tacgtctagt gtgccgtttt ttaggtcaat atcagaccac
    12421 tctaaggcta gacactcacg aatgcgcaaa ccagtcgcta aaagtgtctt atatagcacc
    12481 gtatcgtaga aatttttgta agtgtttggc agctgctcca ggtaagtcaa gaattttttt
    12541 aagttatcat catccagata tttcagtttt tgcccttctt tttccttacg acgtggcacg
    12601 ataacatcac gggcagggtt aaaggggata gcttgcaaag atacggcata tttaagtatg
    12661 cgtttgttta gagagtgaag gagcggatat tcttggaatc ctttgcctag ttgattatag
    12721 tcttttgccc attgattgac ttgcttttga atgattgggg gcgtcagttt atccagccta
    12781 taatttccga aagctggaag tagatattta tctattttga atttggtacc cattaaggtc
    12841 gattttttaa ccgtatggca gtacgtttct agccaattct ccaccagttc ggcgtaagtc
    12901 tttatctcaa ccgccttata tactgtagac cctccttttt caaagtctat ttgagcctgt
    12961 agcgctttgg ctttgagttc tctcttggtt cgccctgata tagtcgtctt gactttctta
    13021 cctgttacgg tatcgatacc aaggtagacg ctagagcgat agactgtaga accgtctttt
    13081 ttggtgtatt ctgttatttt catgatgata aacctttcta aaatacatca gcaggcaagc
    13141 tattattaaa aaggttttag attatggttt atatcatgtt gagcttacgg gaaagatttt
    13201 atttgttatt ttcttcagct aaagctatct gtacaagttt ttcttttact ttttcagtga
    13261 gttcctcata gtagagaatt gaagaaataa tttcttttaa aaaattgaaa tcgattaacc
    13321 ccattgctac ccataagaca atattttcca ttgtttcgat aaagaaggga atgaggtaat
    13381 caaggtgatt gattgttaaa aatttaatac cgaagacaat gctacttctc ttatttccat
    13441 cattgaacat atgaaattga actgtattaa atactagcac tgtaagtttt gtttcaaagt
    13501 ctggatagta atcgtcattc tgtaagtgtt caaatacact aaataccctt cccttatctt
    13561 taacaccgtg taaaccacct attttctcaa ttacttcaat gtcgtgtagt aaaattgctt
    13621 cttcagcaga aaaataattg attttatcca ttatttatct ctcaatcttt caaaaacttg
    13681 gcggtgttca tcaatttgtt ccgaaagtga tttactttgt tcgccaataa atctttcaaa
    13741 atcttcttca gaaattggac ggatatattc tgctaatttc tcatgtttgg cattcctgaa
    13801 agatagatcc ctactagcca ttttctctct ggcatgcatt aaatggggtt cttgcattgg
    13861 gtgatttgct aatctctgaa ttatctcgat tacatcagta ttagttagtt tttgatttga
    13921 agacttactc ttttgttcaa tctcataagc aacccctttc tcaaaactag caataactat
    13981 aagtacctca ctatacattg ttgcgcgagc attatcattt ttattcaatt tcagtatttt
    14041 tttatattct gatgctcttt ctttaaagat tgctttgtaa atttcattcg ttaattgacc
    14101 atatttaaaa ttacttccag agacatactt atcaatggca tttgtaaata tttttctagc
    14161 attatcttct tctaatgcag tcgatagata atttttgtca cgctggttta taaattttac
    14221 atggccacca gtcttctcag ttaacacatt aatgacaata tcaagtaggg tatttcttac
    14281 ttgcttagct atatcactat tggttaacaa catagcaaaa ttaagcaatg ttctgaacga
    14341 tgagatactt aattgtgtaa ctttgctacc gacatttgtg tcggtagcaa aagcattttc
    14401 tttaaattta gctagttttt ctccacgtaa agtgtgataa ccattttcag taagctccgt
    14461 tttatgagct tcaacaagtc tttcaatagt tcgaatgtcg acttcgaaaa aggtagctag
    14521 ctgagaattt gtaaaataaa aagtatcctc aaagtagaaa ccaggtactt ttaaaatctc
    14581 ttcagctttt tgtaatgcta aagaattatt taaaatattt tgtctttcta tttgtgaggt
    14641 tgtcagatca gtcataattt ctcctttttg tcgagttaat ttgccaataa ctcccgatat
    14701 ttcaagagtt ttattttatt ttcgtgtcgt tagagtatat tagaaaatct tgaactccgc
    14761 gaatacacgc gcttcaggat tgttgaacat tgtggcgcgt gggttagtcg ctgaagtgtt
    14821 ccctgatttc ttcagctaaa tcattcaagc ggttctgaat acctttatag taatcaaatt
    14881 caatggtgtc aatgatgtca cttttttcag taaatatctt gttttttgta acacaaaagc
    14941 tattatttgt attattgtga acaaggtcat ttatctcatt ttgaacagaa gatattttcc
    15001 tttgtaagca atagcctaag atttttcgat cttgaatacc tattttttca attatttcat
    15061 tcacttcaaa gacaactgcc ccatagctat caattttatt gttaaggcta ttgataagat
    15121 tagaaatttc agaatgcaca taggaggtat tgagagcttg ctcacgttca ataacgttta
    15181 ttaaacgtgt aatatattcg ttagtaatca tggtgagtac attattataa gcgtactccc
    15241 tagcttcgcg ctgtttggaa agtagtagag cctcattatt ttttgagata tctgataaat
    15301 caagtccgtt gaaaatttca acaataccag ggtttattat tgaagactga agaacgcgac
    15361 ccaaaacacc ctggtcaaat gctatttttc ctcgaatgta ctccatattc atataatctt
    15421 ttttaaagct aagtatttct ttgattttat tgaagaacat ctcaaaaacc ttttctcttt
    15481 gattattatc tagcaactct tttagtgtgc tggttgaaac tgtatcaata aaccgcagat
    15541 ctaggaaaga tttttttacg ttcaaaaaat cagctatttt ttgtaaattc ccagcggttg
    15601 gtaaagattt tccttttaca tatcccgtaa ttgtactttt aggaatatct aaagcgttat
    15661 gaaggtcaat ttgtctaacc cctttttgtt ccataagaag gttgagccgt ttagagaagt
    15721 attctctatt ttcaagatct tgaggactat ttttagccat gactttaacc ctttcagttg
    15781 taagttatac attgattata acacaaaata aaaacaaagt acgaaaataa ataaactttt
    15841 ttctaaaaac acttgactaa gtacgaaaaa gatagtacat atataccgtt tgaactttta
    15901 aaagaattgt ctgcaattta ccaagttaaa ttggacaata tttttttagg agatagtacg
    15961 aaaaaaatag aactaaataa aagagagttg caataatgag agtattattt taagtcaatg
    16021 gtggcagaaa tcaaaccaga cataacaata gatgaattat tttatagcta gaaaggagca
    16081 aaccaatgga actagtctat atggacggta aaaaagagcc gtatacactg agcagtattg
    16141 tggcagagtg tgcagaagtc aagcacagac atttgaagat tttgctaaat aaacaccgag
    16201 aggacttcga gagtttcgga aaggtgcaat ttaaaatttc accttcagaa agtgggcaaa
    16261 atgtacggga ctatatttta aatgaacaac aagcaacatt gctgatcact tacttacgaa
    16321 atacagaacc cgtaaaagag tttaagaaga acctagtcaa agcattcttt gaaatgcgtg
    16381 aggaactttc taagtttcgt atgcagaggg cgctagaaaa gccaaaaaga aaaaccttgc
    16441 atgacagtat taaaacatgg gaggcaaaac ccaagcacgc gcatagcacc atgaacaacc
    16501 tgttactgaa ggcagtaact gacatgaacg ctaagcagtt agtaaaaagc cgtgggggct
    16561 ataatggcat tgatagcttg accagtgacg agctggagca ataccaggca tttgaggata
    16621 tggtaatagc catgattggc ttgaatatga gttatcagga aatcaaagct atggcattca
    16681 gaaataaaaa aaacacgcca aagaagcgtg tgagagcaac aaaaaaggct tagcaataac
    16741 cacaccgcag agcctttcac actaacacta aaacgaattt aacaaaaagc aggcaagcta
    16801 ttatcaaaga ggttttagta aagatttata cctagattat agcatattta gggcaatttg
    16861 accatacgga gagcgccaac tctttaaact ggtactttct cacgctttca atttggcgag
    16921 tctgagcgtg aggctagtac ttacaagaaa aaactgtata agaaacaact caaaaagccc
    16981 cacgctcaga agtttggcga ccgagagcat gaggctgtag gcaagaaaaa ctgtataaga
    17041 aacaaccatt caaaatcccc acactcgcca tcgctcaact ttgagtgtga ggatcagcat
    17101 gtatagtaaa aggtattaaa tggcccttag ggcaactaca caacatagaa acagaggtaa
    17161 aaacatgaca gaaacaacat acgagaattt aactagacgt ataaacagaa ttagcgcaga
    17221 aatgcaagaa attggcgaaa aaaatgggct tagtagactt tcgttattgg caagtcagac
    17281 aaaattcatc aaagaagaat tatcccgttt actttggatt gaactccctg aattgaatga
    17341 cagccataaa atcgaagcgg tctctaaaag cactacggga atgtttttcc accctggtat
    17401 ttttgaaatg gacgctatgc gacaagcatt ctttaaacgc caagctatgc acttttttga
    17461 gaacgaagca gagcaacagg cgtatataga acatgctgaa aaggagtatt tagaggctac
    17521 tataacctta aaagatattc tttttaactc taaaaatgga actcagagag taaataaaag
    17581 ttgtcttata gagaagtttg aggaggcaat gcaatgacac tagacctaga caacatgaca
    17641 caagcagaat ttgataaaca aatggctgaa atcaaggaga gaaatccaaa catttttcaa
    17701 ctgatttctg attttttaga tagaaaggta acacccgaag aggtggatga gtttttgaag
    17761 atggagcgag ctgaacaagt ggaatatatt aagaattatc aagcgagggt ataacatgaa
    17821 tgagctagat ataagtaaca cacaggctgc tgtcttaatc gtgattttga ttggcttact
    17881 gctttattta aaccaccgag accgcaaaaa aagcgcccaa attgagcgag aaaaccaaca
    17941 gacgataaaa acacctagcg aggatttaaa ccctgattat gggcgatata tccagcttgc
    18001 aggagtcaac gtatggggag ggatggaatg agttacacaa tgaatttata ccccgatatt
    18061 gaaaaaatac cagatgaaac ctattttact aaagagattt ttctagcaac gtgcttgaat
    18121 aacacaatca gaatagcagt agccagagga gtgctaacat ctcaactttt agacaaagaa
    18181 actaaagaag ttgtttgtaa aaccttggat acaatcatag aaaatcaaaa agctattttg
    18241 caaggggtag cgagcggaca aataaccttt gtaaataaaa gcaaaggaga ggaggggttg
    18301 gatgagtcta tcagagaatg acaagcgagt tttaagacta atcaaggtag gggctgagaa
    18361 ctccataaca gggtcagaaa ttagcctgac aaccaagcta acagaaagaa ctgtacgtga
    18421 cattatcaag cgtctagtag ttaagcacaa catccctatt gtaggggtta gatgtggcgt
    18481 ttttagtggg tactttattc cagcgaataa aggcgagtta ctagatggcg ctaaggcttt
    18541 ttacaaccaa gtacaggagg agagcaagcg cctagcggtg ttgatgaata gcgacctaga
    18601 aagctacaaa gaaaccttga aggaggtggg cggatatgtt tagcctaagc cgagaaagcg
    18661 agcaagacct agcgcatggc attctggagg tagtggaaag atacctggaa gcgcgtgaga
    18721 aagtaccgcc aagattgacc aagttaataa atagagttga actcaaagag gaattgaaaa
    18781 ttagtgacaa cacgctgaat aagtgggaaa gtcagggttt aagacgctat caaccgccgg
    18841 acgatggtgc tagaataata ttttatttag tggatgacat tcgtaggttt atgggggtgg
    18901 agaatgggta gatataacac acacccagcg caaggcggag gtcattatca tgacattaaa
    18961 ttgtataagc accgtggcag atctttggaa cagttccaag aacaaaagct actgaagaag
    19021 ttaaaaaaaa acgcaaaaag gggcgatagt atgacaattt atgaggctag aggtttccag
    19081 agtaatcttg tctatccatt tgataagatg gagccgttcc agtacattga acgctttaag
    19141 cctttggtag tccctgaaag tgctgatccg gaggaataca aaagaactca ggcgccatat
    19201 tgtttaagtg gtaaggttat gccggagaaa aacgggagtt acaaacgaaa caactccagt
    19261 ctaatctatc gggatttgat tttccttgat tatgacgata tacaggggac gactgaggac
    19321 ttcatagaag ccgtttctag cgctttgttt ggctactctt atatcttata cccgactatc
    19381 aaacatagca tagaaaagcc ccgttttcgc cttgtggtga aatctaacaa cgtgatgaac
    19441 gaggcaacct ataagcaggt agtaaaagag attgctgaca agattgggct accctttgac
    19501 atggccagtt taacctggtc acaactccaa gggctaccag taacaacagg cgacccagca
    19561 acttatcaaa agattgtaga gcatggactt gattatccag tccccaaggt tgaaccaaga
    19621 gcgaagcaag gaactactga gcgttataaa cctagagtaa gtggccagag gtcaatgact
    19681 atgaggatca ttgacacgct tttcaacgga tttggagacg agggagggcg taacgtggca
    19741 gtgacacgct ttgtgggatt actcttcaat aagtgggttg attgtgacct agagaccgcc
    19801 tatgaactga caaagatagc caatagtgtg acagttgaac cattgcctat tgaagagcta
    19861 gaccgcactt ttagcagtat agcacgggct gagtatagaa agagaggtta gaaccataga
    19921 actagaagaa ttagcaaacc taaaaagtga aatcatggag gtaatggagc aagaacaccc
    19981 tcctagaacg atgaaggagc ttgaaaatcg tatctttaaa gctggagaag aatggcgggc
    20041 agaacatact gaaactaagg taaatgagac cacaggggac gttaccgaaa aggttgccat
    20101 gcctcagaca ttcacagtag caaaaatact gagcgagatt gtcacattta cttttatcag
    20161 caagagcaat atacctgatt atagcctgct ctatatctat gatttagacg agggtatcta
    20221 taccgctagt aatgatctat tcaatctttt gtgtaagacc tttgacgtta gaattaaacc
    20281 cagagagtgg ccacagatta agttaatggt tagaacattg gcaaagatac gaaaaccttt
    20341 agagagtgct aaccttgtac ctgtaaagaa tggcattatt gacctaagaa ctaaggagct
    20401 actccccttt agtcctaagt atgtaattac aagtaagata agcacggcct accacgcgcc
    20461 taaacgagtc ccaacagata gagagggcaa aacttttgat gattggctaa actcaatcgc
    20521 ttgtaatgat agtgaactgg taacactgtt ttggcagatt atccttgaag ctattaaccc
    20581 taaccatacc cgaaataagt ttgctatctt ctacggggac ggtaacaatg gtaaagggac
    20641 attccagcgc ttccttatca atctgatagg ggaaagtaat atatcagcct tgaagcctgc
    20701 acagttcgga gaaaagcata acttggagac cttggtaggt aaggtttgta atattgggga
    20761 tgaagcgcct aacgaatatt taaaaaatcc gtctgattta atgagtatca ctagtggcga
    20821 cactgttcta gtcaatccaa aaggacgccc agcctttgaa gctactttca agctattcaa
    20881 tatcttttca gggaattata ttcccaatgg tggcaacaag acaaaaggct ggtatcgtag
    20941 aattatgatc gtccctttta atgctgactt taacggggag aaggaaaaac cttggattaa
    21001 aaatgagttt ttagcaaaca aggaagtcct agagtatgcc ctctacaaag ctatcaatca
    21061 agagccattt actcacttta tcgaacctaa agcagttaaa ggactcctag aagaatacca
    21121 ggaggataat gactacttac tttcatgggt taagcatgaa tacatggaaa aaggttggca
    21181 tgagctggac gtagtacctg tttttatcgt tacaagatca ctaaagcact atgccgaaga
    21241 tatgggaata cccaaaccca atgtttacgg ggctggtaaa gaaactatca agcacttaca
    21301 acagttgaca cctaatcggt attctctaag aaagaaaaga gttccttcag aagattttga
    21361 aaaactagag gcatgggacg ttgacaagat aaaaatgcgg acacctcaac actctatcac
    21421 taaacaagaa taagtgtgac gttctttttt tgcttaaacc cttgataata ctgattttct
    21481 taaaaaaagt gtgacgttgt gacattcttt ccaactctta catatagaga aaaatgcagt
    21541 atttatatat aggaacgata aaacaaaaga acgtcacagg gtcacagggg tggtgaaaca
    21601 cctagagcct caagggtttg aacgtgtgac gttctttgtc acagaaggtc acagaacacg
    21661 aaaaatatta cataatttac cttaaaataa agaaatgaca acaacatgaa agttaaacta
    21721 tttgaatata ctagacgatg ggaaaaagaa gactttgaga aagagatcaa tgacttcatg
    21781 gcaactgttg aagtgctgga cgttaaattt tctggtacac catctagtga atcctttaat
    21841 tcggtggcta tcgtattggt attatacaga taacaaaatg gggtagatag taccgacccc
    21901 cttgtaatgg tgtaactact agtgacacct ttaaaacaga aagaggaaaa acaacatgac
    21961 attaaaaaca ttttcggata aagccaaaac atttactttc acttacgaat ttaaagacct
    22021 agatactgct atggtagcag gtcacgcgct actgggatat atgactggga cttatgaagt
    22081 accatctatt tcaataactc acaaggataa agggacgctt gtcgctgagt atgtggaaga
    22141 tcacaagcta aacaagactt tcaagcgtat ttgtgacagt ttcaaagact attacaacca
    22201 accagtggac gatgaagcat ttgaagaacg ttataaacgg gagcgcgtgc tccaactcaa
    22261 agagtcagag gactttgaga acttgctgga aaaagtaaca gactacgagc tggaactatt
    22321 agactatgct gatcgcttgc tttctgataa gcctatccct atggactcta tgaccgcttt
    22381 tggtacgttg gaaatgctgg gcgatgaaag tattagccta ctccaaaagt tggacgtaga
    22441 ggtcgaatat aaaggccttg ctggttatac agagcatttg aagtagaaca caggggagca
    22501 atcccctttt tgttatcaag tcaatagttt tgggtttttc catatagggg gaaataatga
    22561 agcggttagc aatagaaaca atcactaaac caatgaagct gagaggaata tctaagggta
    22621 tagctgagtt ggacggtcaa cgtttagaga ttgatttgga taatttaatg attgattttg
    22681 gtggagagtc atttgaatta gacagaatag caggaactaa gggaggtaat agatatttct
    22741 ttctttgccc tgattgtggg agacggtgca ggttgcttta taaacggtat ttatatttta
    22801 gttgcggtac ttgtctagat atccacaaga gcaccctgaa ccgtagtaaa acggattgcc
    22861 aatactactg ggagcttgca ctaaaagaag ccagaaaggt agaaccaggg tggagtccta
    22921 gacgtggtgg atatatgttt gatagtttcc ctgaaagacc aaagtatatg aagcgtgaca
    22981 ggtatcataa gcactataag aagtttttga agtatattga aaaaggagat agattttggc
    23041 tgaatggttt gagattgtag gacaatatga agtagtgtgc aatttacaaa acaaacaaat
    23101 actacatatt tgacggtttg aatgttaaaa aagctagata tatcaaggat ttaagtaagt
    23161 agagtgttcc aaaagagggt gaggtaaggg tgaggttaca tataaaaagt acaaggttag
    23221 tacaaggtca catacttcaa aatagcaagg ttttagcaag gtacagggct tttttctagt
    23281 gatagaaccc taatattacc ctaaggtata gctacttttt tagaaccata gaacggtaag
    23341 gttacggtaa ggtatcgaag ttttccaatg gtgaggtaag ggtgaggtaa gagcaaaaaa
    23401 tgtaaaggtt ttgtcaaggt tgcgtattta aaagccccaa ggttaccaca aggtagagag
    23461 gtttaccgag ggttagaaaa ggtgagattt tgcttaggta gtggcagtaa atatgcctgg
    23521 aaaatggcag ggttatgtgt gttgaactcc gagcgaacgc cgagaaagtg gctatatctc
    23581 cctccccacg cacttctatg agcttcacac cgtcattgta cattttttct cacggggaag
    23641 catttgaagc tagagcgaac atagagaaaa cgaaaaaagc caagacactc tctgtctcag
    23701 ctaatagttc tcacaaagac tattatatca tagaggaggc cgaggcatga caccagagca
    23761 ggtaaaagaa aaactagagg gcgttaagtg gataaacaaa gagatagaag gcttatattt
    23821 agagcttgca gctttagaaa gtggtattat caaaaagcaa gaactaagca ataccagagt
    23881 acaaacaagc agggtaaata cgaccgagaa taaccctata agtgttctaa aactaaaaga
    23941 ggatacactt cagagaattg agcgacttaa tgaagagaga atggaaatat ctaggctaat
    24001 cgataagcta gccaatccgc ttgaacgttc tgttctcagg ctgttctact tgaatgttct
    24061 tgacgcttgg caagttgctg aggaaataga taaatcaaag actgcagtat acattataag
    24121 gcaaaaagcc atagaacact tagcaaatgt ggggtaatag gtgagcacaa aaaaacgcct
    24181 agaacaatct aagcgctaaa gagtagtgga cggtgtgcct gtcccgtcat ctcatactat
    24241 gaagttgcgt agcgacacta tcatctctac ctcacttctc tttagactat tataccacaa
    24301 aggagcgaac taatggagct ggataagttt aaaacgatga tgaacgtcag agagcggatg
    24361 acctactttc tacgtttcca gaggatggca ggaagtgaaa accaagttac gatagatgaa
    24421 gaggcttgga aacttgtctt accttatcgg tgggatttga gcggtgagca tgaaaaagca
    24481 atccgtgagg ggttggaaat attcgcccaa gacatcaaca gtatagaaaa caaacgagct
    24541 agaaaatact ttattatcca ttactgttac atgagaaaga aaacaatgag cgaatgcgta
    24601 gaaatggcag ctactagctc cactagctac caccgatata aacaaatagc cgtcttaaat
    24661 tttgcgagaa tccaccagaa cggagagcta gaagtgtata agtaggcgtt tgtggtgttt
    24721 tgttgagaaa tgttgagatt taagtgaggg gggatgacat gattgaagat tttttgattg
    24781 atttggagaa tatccaatta gaggatatgg agatagattt taacatggat atagacatcg
    24841 atttaaacat agattttgaa atagatgttg accttgatga tttagattta accttactac
    24901 aagaattaga agcgtaaatg ttggcaaatg taggctgagg cttcatattc gtcccctatt
    24961 ttgctttggt ttggactgga caattaaaac accatggata aactagaagc cctcagaaac
    25021 gagtctaggg gcttttgtga ggtcataatc ttataaacca cgcgcttcat ggtataatat
    25081 acctatcagc aaccagcaat aaaaaaagca cgtttaaccg tgctagtttc ttgcctgctg
    25141 aactcgtcat ttattgccct tttgtggggc tttttttgtg gactttttga gaatttttaa
    25201 gaacttttaa tgatttctaa tcaaatgaga tttttcaaaa agtcagtaat atcaagggtt
    25261 taggcgcttt aaacaagttt aaaagcgtat agattacaat ctaacacaaa aagggataaa
    25321 gtgctaaccc tctaactaag ggttctcagt caataatttt attcattttt cgctcaaaat
    25381 catatcgccc catcatgaca acatgctcac aattactgca ttttattttg atatctgctc
    25441 ctacacgtgt aatttcccaa cgattagcct ttttaccagt cgacttgatt gtacaagcgt
    25501 gtgatttttt catctcaaca aaatttccaa cttgatacat actactctcc ttttcttttt
    25561 cgattatatc ataaaagagg cgagccaggc tcaacctctt tcacttaatt tgtacgaact
    25621 ggtgtaatga gctgcatgaa gtcttcgtta gtatctgctg gcacaagagt aaatggacga
    25681 acagctgaga taaagctaat ggtcaccttt tcgctattta aagctttaag agaatcaatc
    25741 aagtaagttg ggttgaaact aatggtcaaa tcttcaccag taacctgatc agtatcgatt
    25801 tcttcgttta ctttaccaac ttctggagag tgaacatggg cgctaacaac cccatcctta
    25861 atttcaagtt tcacagtacc attttgagtc gcacttgata aaagacgggc acgctccatt
    25921 gactggcgta agtttaccac attaaaagta atagtagtgt taaagtctgt tggaatcaaa
    25981 cgatctgtat caggatagtt tccttctagg agacgagtat agaagctaat attttcgctt
    26041 ctaaagagga tttggttatt ggcaaagaaa atctctacag tttcgatatc atctgtaaat
    26101 accgctgaaa attcgcgtag agaacggcta ggaattacga catcaaaatc atcactattt
    26161 ttttcaagag tcaatttttt ctggcttagg cgatgagagt ctgttgcaac tgtttttaac
    26221 tctttgtgtt gactcaatac gaagtggaca cctgttaaaa tcggacgact ctcttgtgta
    26281 cttgcagcaa aggctgtttc attaataatt ttcttgagta attttgtttc aagtattaaa
    26341 ggagtgcttg ctgaaatttc ttggattcgt ggatattgtt cgctatcttt tccttttagg
    26401 gtaatttctg atttgccact ggttaaaaca atttgatttt gttcaatttc tttaaaatca
    26461 agagttacat caggtaaact agatactaca ttgataaaga aagaagcttc aagaaggatc
    26521 gaacctaaag aagtaattaa caaaccagca tcttcatttt tttgagaaat aaaattttca
    26581 attgaaattt gaccatttga accaattaaa gtaatacctt cattggtcac gtcaattttt
    26641 actgttgata aaataggaat ggcattttta gaactaatag ctctcttagt agtatttaat
    26701 gcttgtagaa ataaattttt attaattgaa aaatgaatca tggattctc
//
LOCUS       DAVKZB010000019        24736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKZB010000019 DAVKZB010000000
VERSION     DAVKZB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.019"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            511..1674
                     /locus_tag="VXA80_000702"
     CDS             511..1674
                     /locus_tag="VXA80_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV0353240.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVTFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1671..2348
                     /locus_tag="VXA80_000703"
     CDS             1671..2348
                     /locus_tag="VXA80_000703"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV0353241.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2338..3192
                     /locus_tag="VXA80_000704"
     CDS             2338..3192
                     /locus_tag="VXA80_000704"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV0353242.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3211..4278
                     /locus_tag="VXA80_000705"
     CDS             3211..4278
                     /locus_tag="VXA80_000705"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV0353243.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4288..5454
                     /gene="aroC"
                     /locus_tag="VXA80_000706"
     CDS             4288..5454
                     /gene="aroC"
                     /locus_tag="VXA80_000706"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV0353244.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5464..6567
                     /locus_tag="VXA80_000707"
     CDS             5464..6567
                     /locus_tag="VXA80_000707"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV0353245.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6578..6916
                     /locus_tag="VXA80_000708"
     CDS             6578..6916
                     /locus_tag="VXA80_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV0353246.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            7008..8291
                     /gene="aroA"
                     /locus_tag="VXA80_000709"
     CDS             7008..8291
                     /gene="aroA"
                     /locus_tag="VXA80_000709"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV0353247.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8284..8760
                     /locus_tag="VXA80_000710"
     CDS             8284..8760
                     /locus_tag="VXA80_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV0353248.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8757..9605
                     /gene="pheA"
                     /locus_tag="VXA80_000711"
     CDS             8757..9605
                     /gene="pheA"
                     /locus_tag="VXA80_000711"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0353249.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSIHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9602..10876
                     /gene="psr"
                     /locus_tag="VXA80_000712"
     CDS             9602..10876
                     /gene="psr"
                     /locus_tag="VXA80_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV0353250.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11155..12000
                     /locus_tag="VXA80_000713"
     CDS             11155..12000
                     /locus_tag="VXA80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0353251.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTKMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDVVIADMDKSYTEYK
                     GEYGG"
     gene            11990..13093
                     /locus_tag="VXA80_000714"
     CDS             11990..13093
                     /locus_tag="VXA80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0353252.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13095..14081
                     /locus_tag="VXA80_000715"
     CDS             13095..14081
                     /locus_tag="VXA80_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0353253.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNSIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14097..15530
                     /locus_tag="VXA80_000716"
     CDS             14097..15530
                     /locus_tag="VXA80_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV0353254.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15559..16593
                     /locus_tag="VXA80_000717"
     CDS             15559..16593
                     /locus_tag="VXA80_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0353255.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16793..17530
                     /gene="mecA"
                     /locus_tag="VXA80_000718"
     CDS             16793..17530
                     /gene="mecA"
                     /locus_tag="VXA80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV0353256.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17681..18967
                     /locus_tag="VXA80_000719"
     CDS             17681..18967
                     /locus_tag="VXA80_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV0353257.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKLTRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18969..19838
                     /locus_tag="VXA80_000720"
     CDS             18969..19838
                     /locus_tag="VXA80_000720"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV0353258.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19914..19991
                     /locus_tag="VXA80_000721"
                     /pseudo
     CDS             19914..19991
                     /locus_tag="VXA80_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            20133..21071
                     /gene="msrB"
                     /locus_tag="VXA80_000722"
     CDS             20133..21071
                     /gene="msrB"
                     /locus_tag="VXA80_000722"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0353259.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21129..22853
                     /locus_tag="VXA80_000723"
     CDS             21129..22853
                     /locus_tag="VXA80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353260.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLLPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGVSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDVLVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22855..24603
                     /locus_tag="VXA80_000724"
     CDS             22855..24603
                     /locus_tag="VXA80_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353261.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTMDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIAEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT         7253 a         4607 c         5597 g         7279 t
ORIGIN      
        1 tcaatttcaa aaacgttaat tcctataatg tgtgtgatcc aagtattttt gatagaacat
       61 atatttagag ttcgaaatag aatgatgcaa ataggtgaaa ttttaattat tgttgcttct
      121 ataatattat ttattgatag tatattgagt ttgtaacaaa aggcttttct ataaaatcaa
      181 attttccaga tatctactga agctgattta tttcttcatc tttcttctac tacctttgtc
      241 actaaatttg acagacacat attgctgata caacctctct acctataatc tcacggttaa
      301 cctgaggcta gtttcatagt ttgctatttg attttcgttg ataaaaagaa atgctcctcc
      361 aagacggaag agcatttttt attgtgattc ttacacattt atttcctagt tttgcaggaa
      421 gtctagtata ccatttttta aaagaagagt aaaaaagcaa gggtagtttc ttttttgaga
      481 attgtctaaa agtttgatat aatggtagtt atgaatagaa taagagtcag caaaagggtt
      541 gaaaagaagc ttgctaaggg gctagtttta ctagaagcca gtgatcttga gaatgtcaat
      601 cttaaggatc gggaagtaga ggtgcagggt caggaaggaa actttcttgg gactgcctac
      661 ctttctcagc aaaacaaggg cttgggctgg tttatcagca aagacaaggt gaccttcaat
      721 caagctttct ttgaaacgtt gtttagaaaa gccaaagaaa agagaaacgc ctactatcaa
      781 gatgatttga caactgcctt tcgtctcttt aatcaagagg gagatggctt tgggggtctg
      841 acagtggacc tttatggcga ctacgccgtc ttttcttggt ataactctta tgtttatcag
      901 attcgtcaga ctatatcaga agcctttaga caggttttcc ctgaggtttt aggggcttat
      961 gagaaaatcc gctttaaggg ttttgactat gaatctgccc atgtttatgg tcaagaagca
     1021 cctgactttt tcactgtttt agaaaatggt gtcctgtatc aagtctttat gaatgatggc
     1081 ttgatgacag ggattttcct agaccagcat gaggttcgcg gtagtttagt tgacggcttg
     1141 gctatgggta aatccttgct caatatgttt tcctacacag cggctttttc agtagctgcg
     1201 gccatgggag gagctagcca gacaacttct gttgatctag ccaaacgttc acgagaattg
     1261 tctcaagcgc attttcaggc aaatgggctc agcacagacg agcatcgttt tatagtcatg
     1321 gatgtctttg agtatttcaa atatgccaaa cgcaaagact tgacctacga tgtgattgtc
     1381 ctagatccgc ctagctttgc tcggaataaa aaacaaactt tctctgtggc caaggattat
     1441 cacaagttga tttcccagag tcttgagatt ttaaatccgg gagggattat cattgccagt
     1501 accaatgctg ccaatgtttc ccgtcagaaa tttacagaac aaattgataa aggttttgca
     1561 ggaagaagtt accagatttt aaacaaatat ggtcttccag cagattttgc ctataataaa
     1621 aaagatgaaa gtagtaatta cctcaaggtg attagtatga aggttagtaa atgaaattaa
     1681 tcgtttcagt aatgccaaga agtttagagg aggctcaggc tctggatgcc acgaggtacc
     1741 tggatgccga catcattgaa tggcgtgccg actatctgcc taaagaagcg attttgcagg
     1801 tagctccagc catttttgaa aaattcgcag gccgtgagtt ggttttcacg ctacgaactc
     1861 gctccgaagg tggagaaatc gacctttctc cagaagagta tatccatcta atcaaggaag
     1921 ttgcgcaact ctatcaacca gactatattg attttgagta ctatagctac aaggatgttt
     1981 ttgaggaaat gctggacttc ccaaatcttg ttttgagtta ccataatttc caagaaacac
     2041 ctgagaacat gatggaaatc ttgtcagagt tgacgatcct aaatccaaaa cttgttaagg
     2101 ttgcggtgat ggctcacacg gagcaggatg tcttagactt gatgaactat acacgaggct
     2161 ttaaaaccct caatcctgaa caggaatatg tgaccatttc tatgggtaag gtgggcaagg
     2221 tctctcgtat cactgcggat gtgactggtt cgagttggtc ttttgccagt ctggatgagg
     2281 ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa aatcagggag attttggatg
     2341 aagcttgatg gctatacacg tttagctgca gttgttgcca atcctattaa gcattctatt
     2401 tctcccttca tccacaatag agcctttgag gcgacagcta ccaacggtgc ttatgtggct
     2461 tgggagattg aagcgagtga cttggcagaa acagtggcca atattcgtcg ctaccagatg
     2521 tttggcatca atctgtccat gccctataag gagcaggtga ttccttattt ggatgagcta
     2581 agcgatgaag cgcgcttgat tggtgcggtt aatacggttg tcaatgagaa tggcaattta
     2641 attggatata atacagatgg caagggattt tttaagtgct tgccttcttt tacaatttca
     2701 ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta aatcaatctt ggcacaggct
     2761 attttggatg gcgtcagtca gatttcggtc tttgttcgtt ccgtttctat ggaaaaaaca
     2821 agaccttacc tagacaagtt acaggagcag acaggcttta aagtggattt gtgtgcttta
     2881 gaatatgttt ctgaactgca agcaaggatt gccgagtcgg atttgctagt taatgccacc
     2941 agtgtgggca tggatggcca atcctctcct gttcctgaaa acatagtctt accagaaact
     3001 ctcttagttg cagatatcat ataccaaccc tttgaaacac cgtttttgaa atgggctaga
     3061 agtcagggca atccagcagt caatggtctg ggaatgttgc tctatcaagc tgcagaagct
     3121 tttcaactgt ggacaggcaa ggaaatgccg acagaagaga tttggcagtc tttaacagaa
     3181 aaataccaat aaagaaaggg agactctcct atgaaaatca gaatcgatat tcctcatcat
     3241 ccttatgata ttcagattga aaaaggttgt atggcccagg ctggtcagtg gttgcgagaa
     3301 ctctggcaac cgcaaaaggt agtcattgtg acagataacc atgtagcctc tctctatgca
     3361 gagaaggtca agctcagcct agaagatgct ggttttcagg tagctgtttt tgatttctta
     3421 gaaggtgaag aaagaaagaa tttaactact gttcagaaag tctatgaatt tctagtcaag
     3481 caaggtctga ctcgtagcga tggaatcgtt gctcttggtg gtggcgttgt tggggacctg
     3541 gctggctttg tagcctctac ctatatgcgg ggtattcact ttgttcagat tccgactagt
     3601 ttgacagctc aggttgattc ttctatcggt ggaaagacag gcgttaacac accatttgct
     3661 aaaaatatgg tggggacctt tgcccaacca gatggggttc tgattgatcc acttgttctt
     3721 gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg aagttatcaa gtatggcttg
     3781 attgaggatc cagaactgtg ggctctcttg acgggactga atggttctgt tgagagtatt
     3841 ttggaacatg cagagacctt gattgaacat tcttgtcagg tgaagcgcaa gatggtggtt
     3901 gaagatgagt tggacaatgg tattcgtctt tacctcaatt ttggccacac tattggccat
     3961 gccatcgaag cgactgccgg ttatggcaag gtcatgcatg gagaggctgt tgccatggga
     4021 atggtacaga tttccaaggt tgctgaggaa aaaggcctca tgccagctgg cattacccaa
     4081 tctatcacag agatgtgtca gaaattcggc ttgcctgttg actatgaaaa ttgggaagtt
     4141 gacaagcttt atcaggctct tactcatgac aagaaagcgc gtggtaacac cttgaaattg
     4201 gtcttggtgc cagagcttgg ttcagcgatc attcacccag tttctctgga agagatgaaa
     4261 gactacttgg taaaataagg agagtgtatg agatatttaa ctgcaggaga atcacacggc
     4321 ccccgtctaa cagctattat tgagggaatt ccagctggac ttccattgac agctgaggat
     4381 atcaatgagg accttagacg ccgtcagggt ggctacggtc gtggtggtcg tatgaagatt
     4441 gagagtgacc aggttgtctt tacttcgggc gttcgccacg ggaagacgac aggggcgcct
     4501 attactatgg atgtcatcaa taaggaccac cagaaatggc tggacatcat gtctgcggag
     4561 gacattgaag accgccttaa aagcaagcgg aaaattactc atcctcgccc aggtcatgcc
     4621 gatttggttg gggggattaa gtaccgtttt gatgatttgc gaaattcttt ggagcgttca
     4681 tcagctcgtg aaaccaccat gcgggtggca gttggtgcag tagccaaacg cctcttagct
     4741 gagctggata tggagattgc caaccatgtc gtggtctttg gtggcaagga aatcgatgtt
     4801 cctgaaaatc tgacagtcgc tgaaattaag caaagagctg cccagtctga agtttctatt
     4861 gtcaaccaag aacgagaaca ggaaatcaag gactatattg accaaatcaa acgtgatggt
     4921 gataccatcg gtggggttgt ggagacagtc gtcggaggcg ttccagttgg tcttggttcc
     4981 tatgtccaat gggatagaaa attggatgca agattggctc aagctgttgt ctctatcaat
     5041 gcctttaaag gggtggaatt tggtcttggc tttgaggctg gttatcgtaa aggcagccaa
     5101 gttatggatg aaattctctg gtctaaagaa gacggttata ctcgccgtac caataatcta
     5161 ggtggttttg aaggtggtat gactaatggg caacccatcg ttgttcgtgg ggtcatgaaa
     5221 cccattccta ctctttataa acctcttatg agtgtggata tcgaaaccca cgaaccttac
     5281 aaggcaaccg tggagagaag tgatccgact gctcttccag ctgcaggaat ggtcatggaa
     5341 gcagttgtag caacggttct ggcgcaagaa atcctcgaaa aattctcatc agataatctt
     5401 gaggaactaa aagaagcggt agccaaacac cgagactata caaagaacta ttaaggagtt
     5461 cctatggcaa aaacaatcta tatcgcaggt cttgggttga ttggagcctc tatggcactt
     5521 ggtatcaaac gcgatcatcc agattatgaa attttaggtt ataatcgtag tcaagcttcg
     5581 agagatatcg ccttgaaaga aggcatgatt gaccgtgcaa cggatgattt tgctagtttt
     5641 gctcctttgg cagatgtcat tatcctcagc ttgccaatca aacaaactat tgctttcatt
     5701 aaggagttgg ccaatttgga tttgcgagaa ggcgttatta tttcagatgc tggttcgacc
     5761 aagtcaacca ttgtggatgc ggcggagcag tatttggctg gcaagtctgt tcgctttgtc
     5821 ggggcccatc ccatggctgg tagtcacaag acaggggctg cttcggcaga tgtcaatctt
     5881 tttgaaaatg cctattatat ctttacacct tcaagcctga caagtcagga cacgcttaag
     5941 gaaatgaagg atctgctttc aggtcttcat gctcgtttta tcgagattga tgccaaggag
     6001 catgatcgtg tcacttctca gattagccat tttcctcata ttttggcttc tagtctcatg
     6061 gagcagactg cggtctatgc tcaagagcat gagatggcaa ggcgctttgc ggcaggtggt
     6121 tttcgagata tgacccgaat tgcggaaagc gagccaggaa tgtggacctc cattctcttg
     6181 tccaatagcg agaccattct ggatagaatt gaggatttca aggaacgttt ggaagtgatt
     6241 ggtcaggcca ttagtaaggg agatgaagag caaatttgga acttttttaa ccaagcgcgt
     6301 gaacaacgcc agaccatgga aatccataag cgtggtggtg tggatagctc ttacgacctc
     6361 tatgttgacg ttcctgatga agaagatgtc atcctgcgga ttttggaact gctacgtgga
     6421 acttccttgg ttaacatcca catcaacgag gaaaatcgtg aggatattca cggaatccta
     6481 caaatttcat ttaaaaatgc tcaagacttg gaaagagctg agcatctcat aacagaaaat
     6541 accgactata cagtcgtcat caaataagga gaagaacatg tcaaatattt acgatagtgc
     6601 aaacgaactc agtcgcggtc tacgcggatt accagaatac aaggctgtta aagcagctaa
     6661 agatgcgatt gcagcagatg ctgaggcaag caaaatcttt acagaatatc ttgcctttca
     6721 agaggaaatt caaaaactag cacagacagg tcaaatgcca gatgcgtcct tccaagctaa
     6781 gatggaaggc tttggtaaac agattcaagg aaatagcctc ttgtcagaat tctttaccaa
     6841 gcaacaacaa ttggccattt acctttctga cattgaaaaa attgtttttg aaccagtttc
     6901 agaattgctg aaataagaaa agaactatcc taaagtcagg aatttttaag gaatttctgg
     6961 ctttttatga taaaataggt aaaagtattg caagtatgag gtccagtatg aaactaaaaa
     7021 caaacattcg ccatttacat ggtagtatcc gcgtcccagg tgacaagtct atcagccacc
     7081 gttccattat ctttggaagt ttggctgagg gtgagaccaa ggtttatgat attctgcgag
     7141 gtgaagacgt tctttcgacc atgcaggttt ttcgtgacct tggtgttgaa attgaggata
     7201 aagatggggt tattaccatt caaggtgtag gcatggctgg cttaaaagcg ccacaaaatg
     7261 cccttaatat gggaaattct ggcacctcga ttcgcctgat ttcaggtgtc cttgctggtg
     7321 cagatttcga agtagagatg tttggagatg atagtctttc caaacgtcct atggaccgtg
     7381 tgacccttcc actgaaaaaa atgggcgtca gcatttcagg gcaaactgaa cgagacttgc
     7441 ctccccttcg cttaaaaggg acgaaaaacc taagacctat tcattatgag ttgccaattg
     7501 cctctgccca agtcaagtca gccttgatgt ttgcagcctt acaagctaag ggggagtcag
     7561 ttattatcga aaaagagtgc acccgtaatc atactgaaga tatgttgaaa caatttggtg
     7621 gtcatttaag tgtggacggt aagaaaatca cagtccaagg gccacaaaaa ttgacaggac
     7681 agaaggtggt cgtaccagga gatatttcca gtgcagcctt ttggttagtc gcaggtttga
     7741 ttgttccaaa ttctcgtcta gtgctgcaga atgtggggat caacgaaact cgcaccggta
     7801 ttattgatgt cattcgtgcc atgggtggaa aattggaaat aactgaaatc gatccagtcg
     7861 ctaaatcttc taccttgact gttgagtctt ctgacttgaa aggaacagag attggtggcg
     7921 ctttgattcc acgattgatt gatgaattgc ccattattgc cctgcttgcg actcaagccc
     7981 aaggtgtaac agttatcaag gatgctgagg agctcaaggt caaggaaaca gaccgtattc
     8041 aggttgtggc agacgcctta aatagtatgg gagcagatat tactcctacg gcagatggga
     8101 tgattatcaa aggaaaatca gctcttcacg gtgctagagt caatacgttt ggtgaccacc
     8161 gtatcggcat gatgacagct atcgcagccc tattggttgc agatggagag gtggagcttg
     8221 accgtgcaga agccatcaat accagctatc ctagtttctt tgatgatttg gagagcttga
     8281 ttcatggcta aggtgttatt agggtttatg ggggctggaa aatcgactat tgcaagaggc
     8341 ttggacccta attaccttga tatggatgct ctgattgaga agcgcctagg tatgtccatt
     8401 gcgaattttt tcgctgaaaa gggagaagca gcctttcgtc aggtagaatc agaagtccta
     8461 gctgatttac tacaaacaga ccaagtcgtg tcaactggag gaggagtggt tatttctcag
     8521 agaaatcgtg acttactcaa gactaataca gataacatct acctgaaagc agattttgaa
     8581 accctctacc tacgtatcgt agctgataag gacaatcagc gaccgctttt tctaaataat
     8641 agcaaggaag aactagtagc tatttttcaa gaaagacagg cttggtatga ggaagtggct
     8701 agtcgggttt tggatgtgac caagctaagc ccagaggaaa ttatagagga actaagatga
     8761 aaattgctta tctaggtccc aagggatcat tttcacacca cgttgtgcag acagcttttc
     8821 ctcatgagga attgcaggct tttgccaaca ttacagatgt catcaaggct tatgagcaag
     8881 gattagtgga ctattctgtg gtgccagttg aaaattctat tgagggtagt attcatgaaa
     8941 ccttggacta tctttttcat caggctcaca tccaagcagt agcagaaatc gttcagccta
     9001 ttcatcagca gttgatggtg gttccaggcc atactaagat tgaaaagatt ttttcacatc
     9061 cacaggcctt ggctcaagga aagaaattca tcgatgaaca atatccagag gctcaaatcg
     9121 aggtaacagc tagtacagct tatgcggccc gttttatttc cgaacatcca gatcagccct
     9181 ttgcagcagt tgcacctaga agttctgctg aagaatatgg attggaactg attgccgagg
     9241 atattcagga aatggaagcc aatttcacac gtttctggct tctaggagct gaaaagccta
     9301 gtattccctt gcaagcacaa actgaaaaga tgagtttggc cttgacatta cctgacaacc
     9361 ttccaggtgc actttataag gccctgtcga cctttgcttg gcgaggaatt gacttgacaa
     9421 aaattgaaag tcgtccactc aagacagcac tgggtgaata ctttttcatt atcgatgtgg
     9481 attataccga taaggacttg gtccactttg cccaaaaaga attagaagcg attggaatcc
     9541 agtataaaat tctgggtgcc tatcctattt atccaatatc agaccatgga aaggagagaa
     9601 gatgagtaaa gaaaatccct taagtcatca tgagcagttg cgttatgatt atttgctaaa
     9661 aaatattcac tacctcaatg agagagaaaa aaatgagttt gcctatttgc aagaaaagct
     9721 aactcttgct aggggaaata gtagctctag cttggaacaa gaaagagaag agcaggttga
     9781 cttaccaagc tatgcgaacc ggagtcgctc acaatccaaa tcacaagcac tctctttccc
     9841 tccaaaaaag aaaagacgga agctccgtct taaacgaatt tttatggtga ttttttcact
     9901 tttggtctgt gtggctttgg ccatggtatt catgtttttg cgtggttacc aagatgctag
     9961 cgcaaagaaa actgctgatg cccgggcagc tcaagtagaa gtctttaatg gtcaggacac
    10021 tagagatgga gttaatattt taatcatggg tactgatggt cgaatcggcc agaacagtgt
    10081 tgagacacga actgactcta ttatggtatt aaatgtcggg ggctcagata agaaaatgaa
    10141 gctagtcagt ttcatgcgtg ataatttggt ctatatagat ggttatagtc aagtgattaa
    10201 cggtagaaaa cagacagata acaagttaaa cgtagcctac gagttaggag aacaagaggg
    10261 gcaaaaaggg gcagaaatgg ttcgtcaagt cttgaaagat aattttgact tggacattaa
    10321 gtactacgct ttggttgatt tccaggcctt tgccacagcg attgacacgc ttttccctga
    10381 tggggtgacg attgatgctc aattttcaac attgaatggg cgtcccctaa cagaagctac
    10441 agtcggagat gatttatatg ctaccgagac tgagtctcca acccaaacca ttaaagtcgg
    10501 aaaacagcag atgaatggct cgaccctgct caattatgct cgtttccgtg atgatgatga
    10561 ggcggattac ggccgtacaa aaagacagca acaagtttta acagcaattt tagagcaaat
    10621 taaagatccc actaaacttt tcactggttc tgaagctctt ggaaaggttt ttgctatgac
    10681 ctcaaccaac gtaccttata ctttcctcct aacaaacggt ttatctgttt tggatggagc
    10741 aaaaaatggt attgaaaaat tgacgattcc agaactgggt gactgggtag atgcctatga
    10801 tgtttatgga ggcttgggct tgctggttga tcaaaacaaa tatcaaacca agctcgctca
    10861 aatgggcttg agataagata atactcaatg aaaatcaaag tgcaaactag gaagctagcc
    10921 gcaggctgct aaaaacacag ttttgaggtt gtagataaga ctgacgaagt cagttaccta
    10981 tacctacggc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataagacaga
    11041 aaaatcaaag tttgatagct atttggatag cagtcaggct ttgatttttt acagtctgca
    11101 atagattttc accccttgtt tgtggtataa tgaaacgata cgatagaaag aataatgaac
    11161 aatatgactg atttaaaagc aattcaggct cgtagtctgg agatggctga atattttgtg
    11221 gccttttgca aggaacatga tttactttgc tatctctgtg ggggaggcgc tattggtgcc
    11281 cttcgaaaca aggggttcat tccttgggac gacgacctag acttttttat gcctcgtaaa
    11341 gattatgaga aattagcaga attatggcct cgttatgcag atgaacgtta tttcttgtca
    11401 aagagttaca aggattttgt tgatcgtaat ctttttatta ccattcgtga caaggaaacc
    11461 acctgtatca agccttatca gcaggatttg gatttgccac atggtctggc cttggatgtt
    11521 ttgcctttgg attattatcc gaaagatcca gctgagcgga aaaaacaggt tcgttgggcc
    11581 ttgatttatt cactcttttg tgcgcaaact attccagaaa agcatggtgc tctcatgaaa
    11641 tggggaagtc gcattttact gggtttgact ccaaaatctc tccgttatcg catctggaaa
    11701 aaagctgaga aagaaatgac taagtatgat ttggctgatt gtgatggcat tacagaatta
    11761 tgctcaggtc ctggctacat gagaaacaag tacccaatca catcttttga agacaatctt
    11821 ttcttgccat ttgaaggaac aaagatgcct attccaatcg gctatgatgt ctatctcaga
    11881 actgcttttg gggattatat gacgcctcca ccagcagaca agcaggtacc gcatcatgat
    11941 gttgtcatcg ctgatatgga taagtcttat acagaataca agggagaata tggtggctaa
    12001 gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga ggtggtgcag agaagattct
    12061 atcaaccatt gtttcaaatc tggatccaga aaagtatgat attgatattc ttgaaatgga
    12121 gcactttgac aagggatatg aatctgttcc aaagcatgta cgcattttaa aatcccttca
    12181 agattatcgc caaaccagat ggttacgagc ttttttgtgg agaatgagaa tttattttcc
    12241 aagactggct cgtcgtttgc ttgtaaaaga tgattatgat gttgaagttt cttttaccat
    12301 tatgaatcca ccactgttgt tctctaaaag aagagaagtc aagaagatat cttggattca
    12361 tggaagtatt gaagaacttc ttaaggatag ctctaaaaga gaatcacata gaagccagtt
    12421 ggatgctgcg aatacaattg tagggatttc aaaaaagacc agcaattcta tcaaggaagt
    12481 ttatccagat tatgcttcta aattacagac aatctacaat ggatatgatt ttcagactat
    12541 tctagaaaaa tctcaagaga agatcgatat cgagattgct cctcaaagta tctgtactat
    12601 cggacggatt gaggaaaata agggttctga ccgtgtagtg gaagtgatac gattattaca
    12661 ccaagaggga aaaaactatc atctctattt tatcggggct ggtgatatgg aagaggaact
    12721 gaaaaaacga gtcaaagagt atgggattga ggactatgta catttccttg gttatcaaaa
    12781 aaatccttat cagtatctat ctcagacgaa agttcttttg tctatgtcta aacaagaagg
    12841 ttttcctgga gtgtatgtgg aggccttgag tctgggactc ccttttatct ctacggacgt
    12901 tggaggggct gaggaattat cccaagaagg acgatttgga caaatcattg agagcaatca
    12961 agaggcagct caggcgatta ctaattacat gacttctgcc tcaaactttg atgtcgatga
    13021 ggctagccaa ttcattcaac aatttacaat tacaaaacaa atcgaacaag tagaaaaact
    13081 attagaggag tagcatggaa actgcattaa ttagtgtgat tgtgccagtc tataatgtgg
    13141 cgcagtacct agaaaaatcg atagcttcca ttcagaagca gacctatcaa aatctggaaa
    13201 ttattcttgt tgatgatggt gcaacagatg aaagtggtcg cttgtgtgat tcaatcgctg
    13261 aacaagatga cagggtgtca gtgcttcata aaaagaacga aggattgtcg caagcacgaa
    13321 atgatgggat gaagcaggct cacggggatt atctgatttt tattgactca gatgattata
    13381 tccatccaga aatgattcag agcttatatg agcaattagt tcaagaagat gcggatgttt
    13441 cgagctgtgg tgtcatgaat gtctatgcta atgatgaaag cccacagtca gccaatcagg
    13501 atgactattt tgtctgtgat tctcaaacat ttctaaagga atacctcata ggtgaaaaaa
    13561 tacctgggac gatttgcaat aagctaatca agagacagat tgcaactgac ctatcctttc
    13621 ctaaggggtt gatttacgaa gatgcctatt accattttga tttaatcaag ttggccaaga
    13681 agtatgtggt taatactaaa ccctattatt actatttcca tagaggggat agtattacga
    13741 ccaaacccta tgcagagaag gatttagcct atattgatat ctaccaaaag ttttataatg
    13801 aagttgtgaa aaactatcct gacttgaaag aggtcgcttt tttcagattg gcctatgccc
    13861 acttctttat tctggataag atgttgctag atgatcagta taaacagttt gaagcctatt
    13921 ctcagattca tcgtttttta aaaggccatg cctttgctat ttctaggaat tcaattttcc
    13981 gtaaggggag aagaattagt gctttggccc tattcataaa tatttcctta taccgattct
    14041 tattactgaa aaatattgaa aaatctaaaa aattacatta gaacgggggt gaccaagtga
    14101 ttgatgggaa acgattatta tttagtttga ccatagtcag ttatgccttg acgctagtaa
    14161 gtggaattgt gtatctgttt aataataata atgttagctt actttctact ttattgttct
    14221 tgttggttag tagcttgatt gcttgttgga atgatatcaa gtattactta atccatttta
    14281 ttttctattt aaccattttt gtatttctgg tatcaagacc gaccattgat tattttaggg
    14341 atggtgcttt ggatacctat catccaatag cctatcgttt tgcctttata gttgtcatgg
    14401 tttcgattct gggcttgaca acaggaggcg tcctggctcg ttacttcata gctaggaaga
    14461 aaataaaagt agcaaatata ggaaattctc taaaagaggt ttatatcaag cggttacgct
    14521 ttgtatcact aggagttttt cttctaactt atcctttcta tttcattcgg ttatttgaac
    14581 ggctcttgta tcgtttgcag acttcctact atgcctacta tgcaaatttt gaaagtaaac
    14641 tgccttattt tacctacatt ttgtctacct ttacggtcta tgcaatgtgt atgtatctgg
    14701 caaccaagcc aaagaaattg caggccacag cagtgcttgt ctcctttatt gcagctaaca
    14761 ctattcattt ggcaattggg acacgaaatc cctttatttt aagtatttta tttgcttttg
    14821 tttattactt tatgcgggag caagctgaaa aaggaaaatg gattgggttt aaagaaaagt
    14881 tagcgatttt tgtaggttct cctattctca tgttagcgat gggagtactc aattatgtac
    14941 gggataatgt ccaagtttcc catacaggtt tctgggatat cttacttgac tttatctata
    15001 aacaagggac tagttttggt gttctggctc gaggttttct atttaacagt agcctccctt
    15061 accgagattt ccgtaatttt acttttggtc ctgttcttga ttattttgca agggggagtt
    15121 tgggagccat tttcggagga aaagcctttg aacatacaac caatagtgtg gaactagcta
    15181 ttgatagtaa tagttatgcc cacaatctat cctatcttgt cttgaacaag gaatacctga
    15241 aagggcatgg tatcggaagt agttatatca tggagttgta taccgactat ggtatgattg
    15301 gagtctttct gcttagtttc ttactcggcg tattatttat agccatgctg caagtagcct
    15361 atcgctcaag gacaatccta tttgctttat ccctactcat cttgaataat ctattcttta
    15421 tgccaagaag cagcttttca gaaagtttct tcaatttatt tacaatgcaa ttctggggaa
    15481 ttgttcttgt gattatattt gtagcaaaaa tgcttacaaa ggaaaactag tatctactaa
    15541 acaaaggaga aaaaaatcat gtttgaacat tattcagtag ctgatttgtt tgcaaatctt
    15601 tataaaaaac gaaaagcaaa tattttagct ctcatcgctt tatttgctct cattgctgta
    15661 ccatttacaa ttaaagcagt taggaataaa aacactgtca aagatacaac aagttattca
    15721 acttatctta tctataaaat cactcctcca gaagattcgg ccaaaacgat tttgaatcat
    15781 caaattggtg gttatagtga tttttatggg aaattgattg atggtaattt gaatggagct
    15841 tatcttttca atgatgtaga acccagtgag ttgaaaaaaa ttgccagtga attagacacg
    15901 acggaaacaa ccttgaaaaa ttcaacgaat gactattggt ggaaaaaatt gaccgtctac
    15961 tatatgattg acgatgcagg ggttggtgtg aaaattttga catcaagtaa agatgccaat
    16021 gacttgttag agaaaaaaat tgatgggttg attgagaaat ttaaacatgc ttatgcaaat
    16081 gtgaaaattg aaaaactgga aaccatcaac tctaaagaat tgaacgcaaa tggtgaaaca
    16141 gcgcttggct taaatgtgaa aaatctgatt cttcgtttag ttgttattgg agtggtttgt
    16201 gtgattttgg ttgtgatggg aaatgtgtta gtttatctct ttaatccaac aatcaataga
    16261 gtaggtgatt tttctcagta tcaaattgat tttgtaacag agattacaac aattgctaac
    16321 ctagcagatg ttttgtcata caaaaatact ggacaggaat tgaccatcgt tagctcaaat
    16381 aaagctatcc tagataaatt gaaacagagt caagaagctt taaaaggaat gcattttgta
    16441 gatttgcagg atgtatcatc tcttttggaa agagatacag tccttcttgt tgaagagtac
    16501 ggagtgactc gttataagaa atttgagcaa agtcttcaaa ttctcagaaa cttaaatcgt
    16561 tctatcctcg gtgtagcaac ctttaaatta taagtattct gatgtataag gtcttcaaaa
    16621 tcggctctta gccgattttg aaggcttttt ctatctgtct gcagaaattt tctcttggag
    16681 tctcctatag aaagatccta gggaaaatct atccgtatag aggagccttc ttttgataaa
    16741 atcgtaaaaa tctagtataa tagatagaac tgaaagtatg aggttactag caatgaaaat
    16801 gaaacaaatt agtgatacaa ctttaaaaat cacgatgtct ttagaggatt tgatggatcg
    16861 tggaatggag attgctgact ttctcgttcc tcaagaaaaa acagaagagt tcttttatgc
    16921 tatcttggat gagctagaga tgcctgatag ctttctggat acaggcatgt tgagcttccg
    16981 tgtgactcca aaacctgata aggtagatgt ctttgtgacc aagtcaaaga ttgaccaaaa
    17041 tctagatttt gaagacttat cggatttgcc agatatggaa gaattggctc aaatgtctcc
    17101 agatgaattt atcaaaaccc tggaaaaaag catcgcagac aaaaccaagg atgatatcga
    17161 agccattcaa tctcttgagc aagttgaagc caaggaagaa gagcaagagc aggctgaaca
    17221 agaagctgag agtaagaaag aaccttacat ctactacatc ctttcttttg ctaagttggc
    17281 tgacttggta gcttttgcca agacagtgac ttttgaaatg gaaacttctg aactctacaa
    17341 aatgaacgag cgctattatt tgaccatttt agtggatatt gaaaatcatc caagcccata
    17401 tccagcttgg ctgttggccc gtatgcgcga gtttgcagac gatagtgata tcagtcgctc
    17461 agtcttacaa gagtatggtc aagtcttgat gagtcacgat gcagtgctca atctgcaaaa
    17521 aatcggctaa tcctttctgg aaagctattt tcagatttta agaagagcga atcgtctgat
    17581 tcgttttttc tttttatact gaaatagtga tttactataa taggaatttt cacaaaattc
    17641 tgttataatg gctatattag aaaatttcga ggagataaag atgacagtta aaattgcttt
    17701 actaggattt ggtaccgttg caagtggtgt gcctttcctc ctaaaggaaa atggaggaaa
    17761 aatcaatcaa tcagcacatt cagatatcaa agttgctaag gtattggtca aggatgaaga
    17821 tgaaaaaaat cgcttgcttg cagcagggaa tgactttaac tttgtaacca atgtggatga
    17881 tattttatca gaccaggata ttactatcgt agtggaattg atggggcgta ttgagcctgc
    17941 taaaaccttt atcactcgtg ccttggaagc tggaaaacac gttgttactg ctaacaagga
    18001 ccttttagct gtccatggcg cagaattgct agaaatcgct caagctaaca aggtagcact
    18061 ttactacgaa gcagcagttg ctggtgggat tccaattctt cgtactttag caaattcctt
    18121 ggcttctgat aaacttacgc gcgtgcttgg agtagtcaac ggaacttcca acttcatggt
    18181 gaccaagatg gtggaagaag gctggtctta cgatgatgct cttgcggaag cacaacgtct
    18241 aggatttgca gaaagcgatc cgacgaatga cgtagatggg attgatgcag cctacaagat
    18301 ggttattttg agccaatttg cctttggcat gaagattgcc tttgatgatg tagcccacaa
    18361 gggaatccgc aatatcacac cagaagacgt agctgtagct caagagcttg gttacgtagt
    18421 gaaattggtt ggttctattg aggaaacttc ttcaggtatt gctgcagaag tgactccaac
    18481 cttcctacct aaagcgcacc cacttgctag tgtgaatggc gtaatgaacg ctgtctttgt
    18541 agaatctatc ggtattggtg agtctatgta ctacggacca ggtgcgggtc aaaaaccaac
    18601 tgcaacaagt gttgtagctg atattgtccg tatcgttcgt cgtttgaatg atggtaccat
    18661 tggcaaagac ttcaacgaat atagccgtga cttggtcttg gcaaatcctg aagatgtcaa
    18721 agcaaactac tatttctcaa tcttggctcc agactcaaaa ggtcaggtct tgaagttggc
    18781 tgaaatcttc aatgctcaag atatttcctt taagcaaatc cttcaagatg gcaaagaggg
    18841 tgacaaggcg cgtgtcgtta tcatcacaca caagattaat aaagcccagc ttgaaaatgt
    18901 ctcagctgaa ttgaagaagg tttcagaatt cgacctcttg aataccttca aggtgctagg
    18961 agaataagat gaagattatt gtacctgcaa ccagtgccaa tatcgggcca ggttttgact
    19021 cggtcggtgt agctgtaacc aagtatcttc aaattgaggt ctgcgaagaa cgagatgagt
    19081 ggctgattga acaccagatt ggcaaatgga ttccacatga tgagcgtaat ctcttgctca
    19141 aaatcgcttt gcaaattgta ccagacttgc aaccaagacg cttgaaaatg accagtgatg
    19201 tccctttggc gcgcggtttg ggttcttcca gctcggttat cgttgctggg attgaactag
    19261 ccaaccaact gggtcaactc aacttatcag accatgaaaa attgcagtta gcgaccaaga
    19321 ttgaagggca tcctgacaat gtggctccag ccatttatgg taatctcgtt attgcaagtt
    19381 ctgttgaagg gcaagtctct gctatcgtag cagactttcc agagtgtgat tttctagctt
    19441 acattccaaa ctatgaatta cgtactcgcg acagccgtag tgtcttgcct aaaaaattgt
    19501 cttataagga agctgttgct gcaagttcta tcgccaatgt agcggttgct gccttgttgg
    19561 caggagacat ggtgaccgct gggcaagcaa tcgagggaga cctcttccat gagcgctatc
    19621 gtcaggactt ggtaagagaa tttgcgatga ttaagcaagt gaccaaagaa aatggggcct
    19681 atgcaaccta cctttctggt gctgggccga cagttatggt tctggcttct catgacaaga
    19741 tgccaacaat taaggcagaa ttggaaaagc aacctttcaa aggaaaactg catgacttga
    19801 gagttgatac ccaaggtgtc cgtgtagaag caaaataaag aatagaatat aggatgggga
    19861 aactcttgac cagaggggtt catatccttt ttgtgaaaag aagtttatac tcaatgaaaa
    19921 tcaaagagca aactaggaag ctagccgcag gctgctcaaa acagtgtttt gaggttgtgg
    19981 atagaactga cgacgtcagc tcaagacact gttttgaggt tgcagataga actgacgaag
    20041 tcagtaacat atatacggta aggtgacgct gacgtggttt gaagagattt tcgaagagta
    20101 ttagttaaaa acgtgataaa ggagaaataa aaatggcaga aatttatcta gcaggtggtt
    20161 gtttttgggg cctagaggaa tatttttcac gcatttctgg agtgctagaa accagtgttg
    20221 gctacgctaa tggtcaagtc gaaacgacca attaccagtt gatcaaggaa acagaccatg
    20281 cagaaacggt ccaagtgatt tacgatgaga aggaagtgtc actcagagag attttacttt
    20341 attatttccg agttatcgat cctctatcta tcaatcaaca agggaatgac cgtggtcgcc
    20401 aatatcgaac tgggatttat tatcaggatg aagcagattt gccagctatc tacacagtgg
    20461 tacaggagca ggaacgcatg ctgggtcgaa agattgcagt agaagtggag caattacgcc
    20521 actacattct ggctgaagac taccaccaag attatctcag gaagaatcct tcaggttact
    20581 gtcatatcga tgtgaccgat gctgataagc cattgattga tgcagcaaac tatgaaaagc
    20641 ctagtcaaga ggtgttgaag gccagtctat ctgaagagtc ttatcgtgtc acacaagaag
    20701 ctgctacaga agctccattt accaatgcct atgaccaaac ctttgaagag gggatttatg
    20761 tagatattac gacaggtgag ccactctttt ttgccaagga taagtttgct tcaggttgtg
    20821 gttggccaag ttttagccgt ccgatttcca aagagttgat tcattattac aaggatctga
    20881 gccatggaat ggagcgaatt gaagttcgtt ctcgttcagg cagtgctcac ttgggtcatg
    20941 ttttcacaga tggaccgcgg gagttaggcg gcctccgtta ctgtatcaat tctgcttctt
    21001 tacgctttgt ggccaaggat gagatggaaa aagcaggata tggctatcta ttgccttact
    21061 taaacaaata aaacagagag tggggcttcc cactttcttc atttctagaa tatgaataga
    21121 agggatttat gaaacaccta ttatcttact tcaaacccta catcaaggaa tcaattttag
    21181 cccccttgtt caagctgtta gaagctgttt ttgagctctt gcttccaatg gtgattgctg
    21241 ggattgttga ccaatcttta cctcagggag atcaaggtca tctctggatg cagattggcc
    21301 tgctccttat ctttgcagta attggcgttt tagtggcctt gatagctcaa ttttactcag
    21361 caaaggcagc agtaggtttt gctaaggaat tgacaaacga tctttatcgt catattcttt
    21421 ccttgcccaa ggacagcaga gaccgtctga caacatctag tttggtcact cgcttgactt
    21481 cggataccta ccagattcag actggtatca atcaattcct gcgtctcttt ttgcgagcgc
    21541 ccattatcgt ttttggtgcc atttttatgg cttatcgaat ctcaactgag ttgactttct
    21601 ggttcttagt catggttgcc attttgacca ttgtcattgt aggggtatct cgattggtca
    21661 atcctctcta cagtagtctc agaaagaaaa cggaccaact ggttcaggaa acgcgccagc
    21721 aattgcaagg gatgcgggtt attcgtgctt ttggtcaaga aaaacgagag ttacagattt
    21781 ttcaaaccct taaccaagtt tatgctagat tacaagaaaa gacaggtttc tggtctagtt
    21841 tattaacacc tctgacctat ctgattgtca atggaactct tctcgttatt atctggcaag
    21901 gctatatttc aattcaagga ggagtgctca gtcaaggtgc tctcattgct cttatcaatt
    21961 acctcttaca gattttggtg gaattggtca agctagccat gttgataaat tctctcaacc
    22021 agtcctatat ctcagccaag cgaatcgagg aagtctttgt tgaggctcca gaggatatcc
    22081 attcagagtt agaacaaaag caagctacca gagataaggt tttacaagtc caagaattga
    22141 cctttaccta tcctgatgcg gcccagcctt ctctgagaga catttccttt gatatgactc
    22201 aaggacaaat ccttggtatc attgggggga ctggttctgg taaatcaagc ttggtgcaac
    22261 tcttacttgg actttatcca gtagacaagg ggaacattga cctttatcaa aatggacgta
    22321 gtcctcttaa tttggagcag tggcggtctt ggattgccta tgtacctcaa aaggtcgaac
    22381 tctttaaagg aaccattcgt tccaacttga ctctaggttt caatcaagaa gtatctgacc
    22441 agaaactctg gcaggccttg gagattgcgc aagctaagga ttttgtcagt gaaaaggaag
    22501 gactcttgga tgtcctagtt gaggcagggg ggcgaaattt ctcaggtgga caaaaacaaa
    22561 ggttgtctat cgcccgagca gtcttgcgcc aagctccgtt tctcatccta gatgatgcaa
    22621 cctcggcact ggataccatt acagagtcca agctcttgaa agctattaga gaaaattttc
    22681 caaacacgag cttaattttg atctctcaac gaacctcaac tttacagatg gcggaccaga
    22741 ttctcctctt ggaaaaaggt gagttgctag ctgttggcaa gcacgatgac ttgatgaaat
    22801 ccagccaagt ctattgtgaa atcaatgcat cccaacatgg aaaggaggac tagaatgaaa
    22861 cgacaaactg taaaccagac gctcaaacgt ttagccgtag atttagcaaa ccatcccttc
    22921 ctccttttcc tagcctttct aggaactatt gcccaagttg gcttatcaat ttacctacct
    22981 attctgattg ggcaggtcat tgaccaagtc ctagtggctg gttcatcacc agttttttgg
    23041 cagatttttc tccagatgct cttggtggta ataggaaata ctctggtaca atgggccaat
    23101 cctctcctct ataatcgtct aatcttctct tataccagag atttacggga gcgaatcatc
    23161 cataagctcc atcgtttacc gattgccttt gtagataggc aaggtagtgg agagatggtt
    23221 agtcgtgtaa ccatggacat cgaacagttg gcagctggct tgaccatgat ttttaaccaa
    23281 tttttcattg gtgttttgat gattttggtt agtattctag ccatgctcca aattcatctc
    23341 ctcatgactc tcttagtctt gctgttgacg ccactgtcca tggtgatttc acgctttatt
    23401 gccaagagat cctatcatct cttccagaag caaacagaga cgaggggaat tcagactcag
    23461 ttgattgaag aatcgcttag ccagcagact ataatccagt ccttcaatgc tcaaacagaa
    23521 tttatccaaa gattgcgtga ggctcatgac aactactcag gctattctca gtcagccatc
    23581 ttttattctt caacggtcaa tccttcgact cgctttgtaa atgcactcat ttatgccctt
    23641 ttagctggag taggagctta tcgtatcatg atgggttcaa ccttgaccgt cggtcgttta
    23701 gtgacttttt tgaactatgt tcagcaatac accaagccct ttaatgatat ttcttcagtg
    23761 ctagctgagt tgcaaagtgc tctggcttgc gtagagcgta tctatggagt cttagatagc
    23821 cctgaagtgg ctgaaacagg taaggaagtc ttgaccagtg accaagttaa gggagctatt
    23881 tcctttaaac atgtctcttt tggctaccat cctgaaaaaa ttttgattaa ggacttgtct
    23941 atcgatattc cagctggtag taagatagcc atcgttggtc cgacaggtgc tggaaaatca
    24001 actcttatca atctccttat gcgtttttat cccattagct cgggagatat cttgctggat
    24061 gggcaatcca tttatgatta tacacgagta tcattgagac agcagtttgg tatggtgctt
    24121 caagaaacct ggctcacaca agggaccatt catgataata ttgcctttgg caatcctgaa
    24181 gccagtcgag agcaagtaat tgctgctgcc aaagcagcta atgcagactt tttcatccaa
    24241 cagttgccac agggatacga taccaagttg gaaaatgctg gagaatctct ctctgtcggc
    24301 caagctcagc tcttgaccat agcccgagtc tttctggcta ttccaaagat tcttatctta
    24361 gacgaggcaa cttcttccat tgatacacgg acagaagtgc tggtacagga tgcctttgca
    24421 aaactcatga agggccgcac aagcttcatc attgctcacc gtttgtcaac cattcaggat
    24481 gcggatttaa ttcttgtctt agtagatggt gatattgctg aatatggtaa ccatcaagaa
    24541 ctcatggata gaaagggtaa gtattaccaa atgcaaaaag ctgcggcttt tagttctgaa
    24601 taagccattc tcttttgaaa gtttatggac gaaaaaagtt gccttcgagt gacttttttg
    24661 ttacaatagc tagaaaaatt gtttactgta atactcaatg aaaatcaaag agcaaactag
    24721 gaagctagcc gtaggt
//
LOCUS       DAVKZB010000020        23587 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKZB010000020 DAVKZB010000000
VERSION     DAVKZB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.020"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(384..1580)
                     /gene="tuf"
                     /locus_tag="VXA80_000725"
     CDS             complement(384..1580)
                     /gene="tuf"
                     /locus_tag="VXA80_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0353262.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2491..3360)
                     /locus_tag="VXA80_000726"
     CDS             complement(2491..3360)
                     /locus_tag="VXA80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0353263.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3584..4192)
                     /locus_tag="VXA80_000727"
     CDS             complement(3584..4192)
                     /locus_tag="VXA80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0353264.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4150..5256)
                     /locus_tag="VXA80_000728"
     CDS             complement(4150..5256)
                     /locus_tag="VXA80_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV0353265.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDILLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5367..5621)
                     /locus_tag="VXA80_000729"
     CDS             complement(5367..5621)
                     /locus_tag="VXA80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353266.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5961..6407)
                     /locus_tag="VXA80_000730"
     CDS             complement(5961..6407)
                     /locus_tag="VXA80_000730"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0353267.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6480..6805)
                     /locus_tag="VXA80_000731"
                     /pseudo
     CDS             complement(6480..6805)
                     /locus_tag="VXA80_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7039..8757
                     /locus_tag="VXA80_000732"
     CDS             7039..8757
                     /locus_tag="VXA80_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV0353268.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8868..9215
                     /locus_tag="VXA80_000733"
     CDS             8868..9215
                     /locus_tag="VXA80_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0353269.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9494..10330)
                     /locus_tag="VXA80_000734"
     CDS             complement(9494..10330)
                     /locus_tag="VXA80_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353270.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10343..10972)
                     /locus_tag="VXA80_000735"
     CDS             complement(10343..10972)
                     /locus_tag="VXA80_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0353271.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10982..11623)
                     /locus_tag="VXA80_000736"
     CDS             complement(10982..11623)
                     /locus_tag="VXA80_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0353272.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11769..12998)
                     /locus_tag="VXA80_000737"
     CDS             complement(11769..12998)
                     /locus_tag="VXA80_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV0353273.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12988..14154)
                     /locus_tag="VXA80_000738"
     CDS             complement(12988..14154)
                     /locus_tag="VXA80_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0353274.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14298..15311
                     /locus_tag="VXA80_000739"
     CDS             14298..15311
                     /locus_tag="VXA80_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV0353275.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15356..15775)
                     /locus_tag="VXA80_000740"
     CDS             complement(15356..15775)
                     /locus_tag="VXA80_000740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV0353276.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15786..17192)
                     /gene="atpD"
                     /locus_tag="VXA80_000741"
     CDS             complement(15786..17192)
                     /gene="atpD"
                     /locus_tag="VXA80_000741"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV0353277.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17278..18156)
                     /locus_tag="VXA80_000742"
     CDS             complement(17278..18156)
                     /locus_tag="VXA80_000742"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV0353278.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18172..19677)
                     /locus_tag="VXA80_000743"
     CDS             complement(18172..19677)
                     /locus_tag="VXA80_000743"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV0353279.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19692..20228)
                     /locus_tag="VXA80_000744"
     CDS             complement(19692..20228)
                     /locus_tag="VXA80_000744"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV0353280.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20228..20722)
                     /gene="atpF"
                     /locus_tag="VXA80_000745"
     CDS             complement(20228..20722)
                     /gene="atpF"
                     /locus_tag="VXA80_000745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV0353281.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20736..21452)
                     /gene="atpB"
                     /locus_tag="VXA80_000746"
     CDS             complement(20736..21452)
                     /gene="atpB"
                     /locus_tag="VXA80_000746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV0353282.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21487..21687)
                     /locus_tag="VXA80_000747"
     CDS             complement(21487..21687)
                     /locus_tag="VXA80_000747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV0353283.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21762..22694)
                     /locus_tag="VXA80_000748"
                     /pseudo
     CDS             complement(21762..22694)
                     /locus_tag="VXA80_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23146..23544)
                     /locus_tag="VXA80_000749"
     CDS             complement(23146..23544)
                     /locus_tag="VXA80_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353284.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT         6989 a         5050 c         4289 g         7259 t
ORIGIN      
        1 attactattg tataatgctt tttgatttga gtctagtatt atactcttcg aaaatctctt
       61 caaaccgcgt cagcttcgcc ttgtcgtata gatgttactg acttcgtcag ttctatctac
      121 aacctaaaaa cagtgttttg agcagtctgc agctaacttt ctataacaaa atgaaataag
      181 aacaagacaa atcaatcaag acaatcaaat cgatttctaa gaatgtttta gaagtagatg
      241 tgtactattc tagtttcaat atattatagt ttgctctttg attttcattg agtattagtt
      301 caatatctaa aaaaagaacc ttgccaagca agattctttt agtgtctgac ttaaataatt
      361 gttcttctgg gaactaaatc gaattaagct tcgatttctg taaccatacc tgaaccaaca
      421 gtacgtccac cctcacggat agagaatgta gtaccttgtt ctacggcgat tgggtgaatc
      481 aactcaacat cgattgtcac gttatcacca ggcattacca tttcagtacc tgctggaagt
      541 tcgattgaac ctgtaacgtc agtagtacgg aagtagaatt gtggacggta gttgttgaag
      601 aatggagtgt gacgtccacc ttcttcttta gtaaggatgt agacttcacc tttgaattta
      661 gtgtgtgggt tgattgaacc tggtttagca ataacttgtc cacgttcgat ttcatcacgt
      721 tgaacaccac gaagaaggac acctacgtta tctccagcaa gaccttcgtc aagttgttta
      781 cggaacattt caacaccagt aacaactgct ttttgagttt cttctttgat accaacgatt
      841 tcgatttcgt cgttgacttt aacgatacca cggtcgatac gtcctgaagc aactgtacca
      901 cgtccagtga ttgagaatac gtcctcgact ggaagaagca atggtttgtc agtgtcacgt
      961 tctggttctg ggatatactc atcaactgtg ttcatcaatt ccataacgat gtcttcgtat
     1021 ttagagtcac cttcaagagc tttaagtgct gaaccttgga taactggaag atcgtcacct
     1081 gggaagtcgt attctgacaa taggtcacgg atttccattt caaccaattc aagcaattct
     1141 tcgtcgtcaa ccaagtcaac tttgttcatg aagacgataa ggtgtttaac accaacctga
     1201 cgtgaaagaa ggatgtgctc acgagtttgt ggcattggtc cgtcagttga agctactaca
     1261 aggatagctc cgtccatttg agcagcacca gtgatcatgt ttttaacgta gtccgcgtgt
     1321 cctggagcgt cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac gtgcgcagtg
     1381 ttgatagtga taccgcgttc gcgttcttct ggagcagcat cgatagacgc atagtcttta
     1441 ggttggttaa ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc tgcagttagg
     1501 gtagttttac cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg tttactacga
     1561 tcgtattttt cttttgccat ttgagtaaaa gcctccaata aaatatattt tatagataga
     1621 cagtaggcaa tacagtctaa ctttccttac tattttatca aatttcagct aaattgcaag
     1681 tgttttacaa cgtttttgtg aattattctt ggtaaaaatt taactaatta atagaaagta
     1741 ctaaatagac ttatccttca aggagttttc agcattatcc atccactcac ttgctgaaaa
     1801 tgtaaacagt ctctggttta tatagtaaaa tgaaacaaga atagggcaaa tcgttcagga
     1861 cagtcaaatc gatttctaac aatgttttag aagtagatgt gtactattct agtttcaatc
     1921 tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt agtttgccct
     1981 ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg actcagcaga
     2041 gtcaatgtag gtcgaagaat agccccccta atcgcttatt ttatagtaaa atgaaataga
     2101 aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag aaagaaggga
     2161 ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa cccatctccg
     2221 ataatatata gtagaatgaa aactgcacaa tttgattgga gaattcaaac caatttctca
     2281 caatgtttta gaaacaaggg tctattattt caactgcaac ctactatagt ccattgaagc
     2341 tggaatagcc cactgagact tctaaaccac tgctggagag gagtttgttt agagtttctt
     2401 tcatttccct aaaaaatcaa aaagagagaa ccagtctgat cctctcttac ttatagatgg
     2461 tgctcactca aatgttaaag gagctatttc ttagagataa aggaatttga atacagctac
     2521 tgccgcaatt gctgctgcga taggtgctac tactggtacc caagaatacc accattttga
     2581 atcgccttta tgctcaccaa gaactgattt tggaaggaaa gcatgaagga gacgtggtcc
     2641 caagtcacgg gctgggttca aggcaggtcc tgtaggtcct ccaagtgatg ttaccaaagc
     2701 cataacgagg aatccaagtg ccaagtgagc cacagaaagt cctgaagcag tgtgtggagc
     2761 cacctgtgct ttaatagcca agtcagaaaa atcaactgtt tgtcctgctt ctgttgcctt
     2821 ttgtttcatg aattgaagca cttcagcacc aaagaagttt ttagtcaaac caagagctgc
     2881 aaagaacaaa acaaatgaac caacaaactc attaatcaaa ccattgacag ttgctgcata
     2941 gcgactttct tttgtaccat ggtcaatact tgaaatagtt gagaaagttc ccaagatgtt
     3001 atttgggttt tcagttttca agtagaatgg acggtatgtt gccacaacta aggcttggcc
     3061 aaagatagcc cccaagactt gcgcgataat gtaaggtacc acttgtgccc aagggaaaag
     3121 accgctaact gcaagcccta gagtgaaagc agggttgatg tgattcccag atacgttacc
     3181 aaacatcaag gctgggatca taacccccat accataacca acagcgatga cgatccagcc
     3241 actttggtga cctttcgtac ctttaagttc aacgttggca actgcaccat tcccaagaat
     3301 gatcaaaatg gcagttccca aaaattcagt ggcatacttc agtgcccatg tgaaatccat
     3361 ttgctagatt ctccttaaaa ttttttagat attccttatt ttatcaattt ttaccctatt
     3421 tagcaactga tttctggaaa tatttttaaa aaaataggat tcatagtttc ccagttcaac
     3481 agacttgtaa gcgtttttat tctttacgaa agacctcgtt ctcaaaacca ggaaaaaagc
     3541 ccacatctgt gagcttttcc tggttcctag attctaaaat ctattatttt tcatcttctt
     3601 ttttcttgaa aaatcctaat cctagaccag ctaacaaggt catgatacca gcactagcca
     3661 aggtagcatt agcttctgta cctgtgtttg gcaattcctc tctcttacct gtctcataag
     3721 tcggaacttc tgggtctgga ttcactggag tttcagtttt tggagtacct ggttctggag
     3781 ttggtttatc tggtgttgga gtctcttgat ttggtagttc tgggtttggt gtcggaacac
     3841 ttggatgctc tggatttggt tgtgggattg gtttttctgg tgttgataaa cggtcatacc
     3901 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     3961 tatctggatc tttgtacttg tcaatcttac cagatataac ctcgtcccag tttcctgttg
     4021 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     4081 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     4141 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     4201 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     4261 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     4321 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     4381 agcttttata acagactcta ccaacttctc ttgcctacct tctttatagt aattggctgt
     4441 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     4501 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     4561 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattat agctggtacc
     4621 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     4681 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     4741 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     4801 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     4861 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     4921 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     4981 ataagaaaca acatcttttc ctaatagaat atccccagta gagaaatagc cgccttttcc
     5041 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     5101 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     5161 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     5221 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     5281 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     5341 ctgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     5401 gtcttcagta gtttatatac tctgtcatca tctttagctt tagaagcagc tgagtttagc
     5461 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     5521 gctagcctat tggttatcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     5581 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     5641 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     5701 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     5761 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     5821 tcaaaccttt cttatacaag tcttcattct gaacgatttc ggctacacgg tgaatggttt
     5881 gattgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgatagg
     5941 ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca ttcaaaaata
     6001 gttggaactt cccttaatac atccgtcact ttctttttca agatagccca agcttgctca
     6061 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     6121 cagagttcat ccaaaatgtt cttgggatga aaaccagcat tgcccatgac aataacatga
     6181 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     6241 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     6301 gaagttcgct caaaacgacg tccgctaatc ttttcataga ctttctcccc tctaggagcc
     6361 cctgcataag gacgatagag atagcggtcg attcctgttt catcaatata aacaggtgct
     6421 aggtgcttta aactattaaa attcttaaga aataaggcta cttttctggg tcttgttcct
     6481 taaagctcgt catctttttt taaagtgacc ttaatctgct ttaaagctgc ccatactgaa
     6541 ggaacagcac aatcaaaatg tgccgcaatt tcccgtaaaa aagcatctgg atgagcctct
     6601 acaaaggctt tcaattcctc taaaggaatc tttcggtttt tgacgactcg ctttttcctc
     6661 tctatgtgtt cttgttcacg tctttctttt cccacgtgaa gagagttctg acgccaacac
     6721 caaaaaactt ggctgcctcg acatggctgt gcccctcttt gatgtaatct aatgctcgct
     6781 gtttaaaatc agtactatat gccatagctt tattataata tgcttgttta aaactaagta
     6841 actatatcaa tgttttgtta tgagaagact tacttttttt actgaaattg aatttttcct
     6901 agcctcaaca tcatataacc ccaaaattca atctgacctg gtttgaagag atgttcgaag
     6961 cagataaggt tttactaaaa ctaaaaaaaa tgatataatt aaactgatta aatgttttaa
     7021 aaataaagga gaatttgaat gtcttaccaa gaaaattacc agaaatgggt tgattttgtg
     7081 gagcttcctg actaccttcg tcaagatttg gaaaatatgg acgaaaaaac taaggaagat
     7141 gccttctata caaatcttga atttggtact gcaggtatgc gtggcttggt tggtgctggt
     7201 acaaaccgta tcaacatcta cgttgttcgc caagctactg aaggattggc tcgtttgatt
     7261 gagtcaaaag gtggaaacga gaaagaacgc ggtgtagcaa ttgcctacga tagccgtcac
     7321 ttctcacctg agtttgcctt tgaatctgcg gcagttcttg ctaaacacgg catcaaatct
     7381 tacgtatttg aaagccttcg tccgactcca gaactatcat ttgcagttcg tcatctcaac
     7441 tgtttcgcag gtatcatggt cacagccagc cacaaccctg ctccatttaa cggttacaag
     7501 gtttacggtg aagacggtgg acaaatgcct ccacacgatg cggacgcttt gactacttat
     7561 atccgtgcaa tcgaaaaccc atttgcagtt gaagttgctg atgtggaaac tgaaaaagct
     7621 tctggcttga ttgaagttat cggcgaagct gttgacatag aataccttaa agaggttaag
     7681 gacataaaca tcaacccagc cttgattgaa gaatttggta aagacatgaa gattgtctac
     7741 acaccacttc atggtactgg tgaaatgttg gctcgtcgtg ctcttgccca agcaggattt
     7801 gactctgttc aagttgttga agcgcaagca actgctgacc cagacttttc aactgtaaca
     7861 tctccaaacc cagaaagcca agcagccttt gcccttgctg aagaacttgg tcgtcaagtt
     7921 ggtgcagatg ttcttgtcgc aactgaccca gacgctgacc gtgttggtgt tgaagttctt
     7981 caaaaagatg gtagctacct caacctttca ggtaaccaaa tcggtgctat catggctaaa
     8041 tacatcttgg aagctcacaa aaacgctgga actcttcctg aaaatgccgc tctctgcaaa
     8101 tctatcgttt caactgactt ggtaacgaag attgctgaaa gctacggcgc aactatgttc
     8161 aacgtcttga caggtttcaa atttatcgct gaaaaaatcc aagaattcga agaaaaacac
     8221 aaccacactt acatgatggg atttgaagaa agcttcggtt acttgattaa accatttgta
     8281 cgtgataaag atgccatcca agccgttctt gtagttgctg aacttgctgc ctactaccgt
     8341 tctcgtggtt tgacacttgc tgacggtatc gaagaaatct ataaagaata tggctactac
     8401 gcagaaaaaa caatctctgt tactctttca ggtgtcgatg gtgctgaaca aatcaaagcg
     8461 attatggcta aattccgcaa caatgctcca acagaatgga acgcaacagc tatcactgtc
     8521 gtagaagact tcaaggcaca aactgctact gttgctgacg gtactgttac aaacttgaca
     8581 actcctccaa gtgatgtgtt gaaatacaca cttgctgacg gttcatggat tgccgttcgc
     8641 ccttcaggta cagaaccaaa aatcaagttc tacattgcag ttgtagggga aaccaacgaa
     8701 gaatcacaag ctaagattgc taacatcgaa gcagaaatca atgcatttgt aaaataaaga
     8761 cctataaaag atgagaccaa ccaacctcat cttttttcgt atcaaatcta agcaatttta
     8821 gaaactttat ggcatactag acttatcaat gaaagcgagg taatctcatg gaacaatttt
     8881 tagataacat caaagacctt gaagtcacta cagttgtgcg tgcgcaagaa gctcttgata
     8941 aaaaagaaac tgcaaccttc tttatcggtc gcaaaacttg cccttactgc cgtaaatttg
     9001 caggtacatt gtcaggtgtc gtagctgaaa ccaaagctca catttacttc atcaatagtg
     9061 aagaaccaag ccaactcaat gatttgcaag cattccgctc acgctatgga atcccaactg
     9121 taccaggttt tgttcatatt acagatggac aaatcaacgt ccgttgcgac tcttcaatgt
     9181 cagcacaaga aatcaaagat ttcgcaggat tgtaaaaaat cagctaacaa gtcttaaaat
     9241 cacaaaaacg cataatgtaa ggtgcaaaaa ccttgatatt atgcgtttta ttgttcctaa
     9301 aacagttaca tcagtatttc attacagaac attttaattt tggaagtcaa atcaattttt
     9361 aacaatgttt tagaaatcac agtgggctat tccatattca attatccata ttcaatcgct
     9421 attatcgttt tcctagatta caaaaaaaac tacgttttgc taatcaaaac gcagtttcag
     9481 ccactatttt atcttactgt ccttctttta cttctttggt tgctacatct tctccaaacc
     9541 atttttggct gatttcttgg aacttgccgt ccttgtaaag actagaaaaa gcttcattta
     9601 tcttcttaac caagtttgta tcttccttac gggctccaac cgcaaaagct tctgtttcta
     9661 gtccaactgt aaagacatta taatcgttta aaacaccttc tgcttctaaa taatagtttg
     9721 catagacacg gtcaatcaat agaccatcaa ttcgatcgtt tttcaaatca atcaaggctt
     9781 cattaaaggt ttggtattga ttcgcttcct tattagcgac aatattcttc aaaatttctg
     9841 gatttgcttc aaagtccgca taaccagatg aaccagcttg agctcctaat gtctttccag
     9901 tcatatcctt tgcagtcgtg ataccagatg atttcttcgt aaccaatacc tgctcattct
     9961 tcatatatga gttactgaaa gccacctttt cacggcgttc gtctgtagcg gaatagccat
    10021 tccaaatcag atcaatcgtt ccttttgtca attcagcttc tttcaaatcc caatcaatcg
    10081 gttgccaatt taccgtgatt ccgtattttt caaaaacagc tgtagctaaa tcaatatcaa
    10141 atcctgcata agaaccatct ttctgagcaa atcccattgg aacaaaagta ctatcaaatc
    10201 caatagtaat agacttgtta gactggtact ttgaccaatt atctccacta gtttcgctag
    10261 aatttttccc acaagctact aagaacaaag cagtcatcag actgactaat acaagcatcc
    10321 attttttcat ccatttttcc tcctatttag gttctacttt caataataca tctgcgatat
    10381 tttcagcaaa ctgcaaatca tgggtaacca caatctgggt catcccaagt tccctatttt
    10441 gcaagattag cttctccact tccaaacgta attctggatc cagggcagaa gttggttcat
    10501 cgtagccaat gatttctggg tcaatcatca tagcacgcgc caaagccacc cgctgctttt
    10561 gcccaccaga tagtgagaaa ggataggcct ctgcgtgtcc tcctagtcct aactgttcca
    10621 agagcccact cgccttcttc tcagcctctt cctgcttcat tcccatggtc ttcacaggcg
    10681 ataaagtcaa attttccaga actgatagat gaggaaatag ttgaaaatct tggaagacaa
    10741 atcccagtag gttgcgcttc tgcaattcat ccagctctaa aggttgtcca ttataaaaga
    10801 tttgccctga atcaatggtt tcaagacctg caagcatacg taaaagagtt gtcttacctc
    10861 caccagaagg tccaacgata gccaggattt gcttttcagg aatacttaga ctgaaatcag
    10921 acaggatttg tttgtctcca aagactttat tgatatttcg taattctaac atagctgccc
    10981 cctatctata ataactatac ttcttctcaa cttttttgga aataattgtc acaatcccaa
    11041 ttaaaatcaa gtaaatggct cctgccaaga acataggaac cagactagca tcgcggttag
    11101 cagctgttcg actagccaag ataaggtctg aaattccgag agcatagacc aaagaagtat
    11161 ccttgaccaa actcataact tcattaaaga cactaggaag aacgatcttg gtcacttggg
    11221 gcaagataat atagcgcact ctgtcaaaag ggctaaactt caagaccttg gcagcctcat
    11281 actgtcctct tggaatagtg tcaattcccc cacggaaaat ttctgcaaag taagctgcat
    11341 agttgagaac aaaggcaata atagctgcag gaaggcggtc taaacgaatc ccaatacttg
    11401 ggagcacata atagataaaa atcagttgca agagtaaggg ggttcctcgc ataacccaga
    11461 tgtaaatgtt aatcagataa tggaggggct tccaatggac ttgcaaggca aaggcaatca
    11521 aaacgcccaa gggaatcgaa aatagcaaga ccagtgcaaa gacctgtaca gtcgtgctcg
    11581 caccattcag taaactcggt aatatctcaa ataaataaga catactgcac ctcctaaaag
    11641 taattgttcc tattatagca tgaataaaca agattaccaa gtatttttgt aaaaagattc
    11701 taccatatac aaataaaaac cgtagctatc aaaagagata aactacagtt ctatatgttt
    11761 aaacattctt acaatctctc aaacagttct tgcatttgca catcatctgg aaccagtttt
    11821 aagtaagtgt gagcatggac ttttgcttct tcaaaatgtc ccaattcacg caagagatag
    11881 atatagtgtt ccagaaattc tggattgtcc ttcaaatctc ctgtcaactc ttgataatgc
    11941 tcataagcag tatccaaatc gtccatttct tgataagaac gagcaatcat ccacttggtc
    12001 aaaagatttt ctggctcctc actctgcaag tctagaatat cctcataacg ctcctgctcc
    12061 agataaatag tggctaaacg aagcaagatt tcttctgtat cctcagcgtc ttcttttgca
    12121 gtaaggagat aattttctgc accactagca tcatgcaatt cataagaaaa ttgtgaagca
    12181 gctagcaaga ggcgagtttc aaagggattt ttctctaatc cttgcttagc gatacgcagg
    12241 gcttcttgaa cttgatgttc cttatgtaaa gcctgactgt acccatactc atagccttca
    12301 aagtcaggag aaatggtatc aagctgctta aagtagaggg tggctttttg atattcttct
    12361 tgatcaaaat aaagactggc caactcaaaa gctgttaagt catcgtattc taactccagg
    12421 gctttttcta aaaactcagt agccgtttca aatttcccta actgagcata ggcaaagcca
    12481 attcgttgat aggtggaaat gcccgtttgc tcataaatcg agcgattatc taactgggca
    12541 taggcttgaa tagccgcttg gtaattttcc aactcactat ccaactctgc caaacccaat
    12601 atcaagagag aatcctctga gtaggtcaag gcctccaata atttctcacg tgccacatct
    12661 gtcaaacctt ccagctggta aaggtctgcc ttcaaaacca aagacgagac ataccagtca
    12721 ctgtcagctt ggatttcctc aagataggta aaggcttctt ctatttgacc atcctcgcta
    12781 gcaattgcag ctagattaag atgaacctct ggaaaatcct ctacaatttt caggtaaatt
    12841 tccttggcct gaggatagaa accaatccct tcaagataag ttgccaactc atacagaaga
    12901 tcacttgaat cattttctaa agctttggcg aaataatgct cagccttagt taaatcttgc
    12961 tcctccaaag cctgtaacat ctgttgacta ttgttcactc ttgacttcct caccctctaa
    13021 ttcatataga ccactgacta ccttatacca ttcgaaaatg gctgaaatga caaccttagc
    13081 agaggcataa accggaatac caagtaaaac tccccagata ccaaacatag atcctgaagt
    13141 taacaaaaca aagagaacat taatagggtg gatgtttaat tgacttccca aaatcaatgg
    13201 agagacaaaa cggccttcaa tagtttgttc tacgataaag acaatcacta ctttcaaaag
    13261 catgactgga ccagcaatca aacccaatac cagggcagga agcatggcta gaaagctacc
    13321 aagataaggg accagattta aaataccagc agtaaccccc agcgtaaccg catagcgtag
    13381 accaataatc ttgaagaaga tgataaacat tactgctaca ataatagcca ctgtcacttg
    13441 ccctcgaaca tagttggaca actgttgatt cacatctgat agaacttgtc caacaggttc
    13501 cttcaatttt cttggaatga attgggtcaa ataattacgc aagcctttcc catcacgcaa
    13561 gagataaaag agcatgaaag gaacgataat caaggcaaca atcacttgag aagccccgct
    13621 aataaaggca ctcacccagt tgactgcctg agatgaaacc ttacttgcca aaactgtagc
    13681 ctggctagaa aaattggtca aaacttgctc taattgaggt ctgaaatcat ctggcaggtg
    13741 ctgggctacc aatccattaa caatcctatc tatatcttct aagtaaacag gaacgtttct
    13801 tgcaaaggtc aaaacctgac gttgcagatt tggaatggcg actgccaagc cccaaatgat
    13861 aaagagagcg ataataacaa agacaatagt gatagctata acacgattaa ccttatgctt
    13921 ctccatccaa tcaacaatag gattcaacaa ataatataac aaaccagaca aaatgactgg
    13981 caacatcaca actgctaaaa agtctaaaac aggtgaaaat agaaaactaa tcttacttaa
    14041 aataaaaaga ttcaatccca ataataaggt tactaaaaat accgtaattg ccttgttatc
    14101 taaaaaccac ttgaaaaacc aagataggct aaaatgtttc tctttttgct ccataaatac
    14161 ttcctttcta ttgttctctc attataccat ttttgaaaca aaataactct tttttaaagt
    14221 gcttacatct aattggatgt ctttaatttg cttgtttttg tgatataata gtcttatctt
    14281 tacatagagg tatagtaatg aaagaaactg tttattttgg aacttataca cgtcgtactt
    14341 ctcaagggat ttacaaggca gactttgata cagaaactgg tcagctttca aatctagaac
    14401 tttttgcagc tgagccaagt ccaacctacc ttgcctttga ccagcaccaa catttataca
    14461 ctgttggtag ccaagacgat aaggggggaa ttgcagccta tcaaactgac gggactgtgt
    14521 taaatcatgt tgttgaagaa ggagctcccc actgttatgt tgctgtcgat gaaaagcgtg
    14581 atttggttta cgcagccaac tatcacaagg gacaagtcct tgtttataaa cgccaggaag
    14641 atggtagtct tctacttagt gatatggatc aacacagtgg ccaaggtcca catgaaaatc
    14701 aagcttcccc ccatgttcac tatacagatt taacacctga ccactatcta gtgacctgcg
    14761 acttgggaac tgaccaagtc atcacctatg acctcgatca agaaggaaaa ttatctaagc
    14821 tctataccta tcacagcaag ccaggagcag gctcacgcca tatcattttc cataaccact
    14881 ataaaatcgc ttatctcatt tgtgaactca atagtactat cgaagtttta atctacgatg
    14941 gcgttggcga atttgaacgt atgcaggtca tttcaacttt accggaagct tacgaaggct
    15001 ttaatggaac cgctgctatt catctctcta aagacggtaa atacctctac gcttctaacc
    15061 gtggccatga ttctatcgca gtatatacca tccttgcgga cggtagctta gagttattag
    15121 aaatcgttcc aacgcatggt cagactccac gtgattttga tttgacaccc gaccaaaaat
    15181 ttctcattgt tgtccatcaa gactctgaca atgcaactgt ctttaaacgt aattgtgaca
    15241 atggtcgtct agcagaactc tccaacgact tccatgttcc cgaagcagtc tgcatccgtt
    15301 ttgctcctta aaacaccata aaaaatgaac ttggtatttt caagttcatt ttttcttata
    15361 gtctatttcc gacattaata cggttaatgg cacgttgcaa agcaatctta gcacgacgtt
    15421 cttggtcaat caaatgtttg tcttgtgctt cttcaattgc acgttctgca cgaagtttgg
    15481 cacgttctgc acgactgata tcgatatcac gagcacgttc tgcagagtca gcgacgattg
    15541 taatcatatc attggcaatt tcaataacgc caccgtttac tgcaatccag ttcacgtgat
    15601 ctttatcatc gatacgtttt acctttactt catcaactgc taaaaccgca atcatatttt
    15661 catgtcgtgg caagatcccc atctcaccat ccagagttcg aaccgataca tagctggcat
    15721 ggtgatcata gacgagacca tctggtgtca cgatctggac agttaactga gccataggtc
    15781 acctcttaaa atcccatttt ttcagctttt gcaatcacat cttcgataga acctacacca
    15841 cggaaggcat cttctggcaa gtggtcgtat ttaccatcaa ggatttcctt aaagccacgt
    15901 acagtttcag caactggaac ataagaacct ggctgaccag taaattgttc cgcaacattg
    15961 aagttttgtg acaagaagaa ctggatacga cgggcgcgag caaccaaggt cttttcttca
    16021 tcagaaagct catccatacc aaggatagca atgatatctt gcaattcatg gtaacgttga
    16081 aggacacgtt ttacttcagc agcaactgca tagtgctctt ctccaacgat ttcaggtgcc
    16141 aaggcacgtg agcttgaagc aagtgggtca acggctgggt agatacccaa ttgtaccaac
    16201 ttacgttcca agtttgttgt tgaatccaag tgagcgaagg ctgttgctgg cgctgggtca
    16261 gtatagtcat ccgctggcac atagatagcc tggatagagg ttacagaacc cttcttggtt
    16321 gatgtgatac gttcttgcaa ttgacccatt tccgtagcaa gtgttggttg gtaaccaacg
    16381 gctgatggca tacgacccaa aagggcagat acttctgaac cagcctgagt gaaacggaag
    16441 atattatcga taaagagaag cacgtcttgg ccttccacat cacggaagta ttcagcgatt
    16501 gtcaaaccag taagggcaac acgcatacgt gctcctggtg gctcattcat ctgaccaaag
    16561 accatggctg ttttctcgat aacgcctgat tctttcattt cccagtaaag gtcattcccc
    16621 tcacgagtac gttccccaac accagtaaat actgaaatac caccgtgctc ttgggcaatg
    16681 ttgtgaatca attcttggat taagacggtt ttaccaactc cggcaccacc gaaaagtcca
    16741 actttaccac ctttaaggta aggggcaaga aggtcaataa ccttgatccc tgtttcaagg
    16801 atttcagaag aggtagacaa ctcatcaaaa gttggagctt ttttatgaat tggctgacgc
    16861 tctgcgtctt ctgtaaaagg agcttccaaa tcaatggtat ctcccaaaac gttgaagaca
    16921 cgtcccaaag tttctttacc tactggtaca gagattggac gacctgtgtc caatacttcc
    16981 attccacgag tcaacccatc tgttgattcc atggcgatag tacgaaccat accatctcct
    17041 aactccaagg ctacttcaag gacgattttt gtttttcttt cgtcattttt gtagacgaca
    17101 agtgcattgt taatctcagg aagtttttcc cctgctgcaa acaaaacgtc tacaacggga
    17161 ccgataacct gagcaatttt acctgaactc atctccttct cctattctat ataagatact
    17221 gtcggttcct agctcaacta ggtcctaagt tcattttcat acgagctgga ctagagccta
    17281 ttctaaggca ctagcacctg ctacgatttc tgtaatttct tgtgtaatcg ccgcctgtct
    17341 ggcacggtta tactgaattg tcaaatcatt gatgactttc ttagcattat ctgtcgctgt
    17401 ttgcatggct gtcatacccg cagcattctc agctgtcttg gcatcgataa tggcaccgta
    17461 aatcatactt tctgcaaact gaggcaacaa ctgctccaga atttcttctc ggctggtttc
    17521 caattcaaaa gtcaagctgt actcttcatc cgcttcattt ggatccaagt caacaatcgg
    17581 aagcatttgt tccacacgca tttgactggt tagcgtattg acatggtggt tgtagcaaac
    17641 ataaagctca tcaaagagtt cattttggta catttcaaca gtttttgaaa taatcttacg
    17701 aacttgatca aagctaggtt ggtctgccaa gccacgtaat tcataaagtg gttgaatacc
    17761 gcgagcctta aagaaatcag ctcccatccc accgatacag atcatttcaa aacctttacc
    17821 gtctgggtgg tattcttctt tcaactccat aacagctttc aaaatagagg aattataacc
    17881 tccaaccaaa ccgcggtctg aagtgataac gatatagcct gtcttcttca cagaacggct
    17941 aatcaacatc ggattagttg aagcaccagc tccattacca tgaaggatat ctgtcaaaag
    18001 tttacgcact ttctgagcgt aaacttggaa gttgcgagca gcttcttcag agcgacctaa
    18061 cttagcagcc gataccattt gcatggcatt agtgatttga ctcgtatttt ttgttgaggc
    18121 gatttttgtt ttaatatcat ttagagacac tgccatctga cacctctatt cttattggaa
    18181 gctagattga ttgagaaact ctgtaatcgc agcatccaag actgcttctt ctggcaagtc
    18241 ttttgtatca cgaatggttt ccaaaatctc tggatgttga gcatcaaaga aggcatggaa
    18301 ctcttcctcg aaacgaacaa tatcatctac tggaacagta tccaagaaac catgtgtcaa
    18361 agcataaaga atggttactt gtttctcaac aggtaatggt ttgtgaacag gttgtttcaa
    18421 gacctcaacg gtacgacgtc cacggttcaa cttagcctgt gttgctgcgt ccaagtcaga
    18481 accaaactta gtaaaggctt ccaactcacg gtatgaagca aggtcgatac gaagtgtacc
    18541 agcaaccttc ttcatggctt tgatttgtgc agaaccacct acacgagata cagatgaacc
    18601 cgcatcgatg gctggacgaa tacctgcatt gaagaggcca tcgccaagga agatttgtcc
    18661 atcagtgata gaaatcacgt tggttgcgat ataggctgag atatctcctg cttgtgtctc
    18721 gataaatggt agggctgtaa ttgatccacc accaagttca tcagaaactt tagctgagcg
    18781 ctcaagcaaa cggctgtgga gatagaaaac atcccctggg aaggcttcac gacctggagg
    18841 acgacgaagc aagagcgaca gttcacgata agctaccgct tgttttgata gatcatcgta
    18901 tacaatcaaa acatgcttac cttgatacat aaattcttcc gccatagcga ccccagtata
    18961 aggagctagg aagagcaatg gagatggttg tgaagcagag gctgtcacaa cgattgtgta
    19021 gtccaaggca ccgtactgac gaagtgtttc tacttgcgta cgaactgttg attctttttg
    19081 tccaatcgcg acgtagatac agatcatatc ttgatctttt tggttcaaga ttgtatcaat
    19141 cgcaatggtt gttttccctg tctgacggtc accgataatc aactcacgtt gaccacgacc
    19201 aatcggtaca agggcgtcaa tagctttcaa accagtttgc aatggttctg aaacagactt
    19261 acgttgcata acaccaggag ctggtgcttc tactggacga gttttatcag tgtggatttc
    19321 tccaagaccg tcaactggac gaccaagcgg atccacaaca cgaccaatca gactttcacc
    19381 tacagggact tccatgattt tccctgtacg gcggattgta tcgccttcac ggatatctgt
    19441 aaagtcacct aggatgataa taccaacgtc tgttgactcc aagttttgag ccataccata
    19501 agagccgttt tcaaaattca acaactctcc actcatgaca ttttcaaggc cgtgagcacg
    19561 cgcaataccg tccccgatat aggttacaac acctgtttca gtcacatcaa aattgggttt
    19621 gaaattttca atttgttgct taattaaagc gctgatttct tgtgcgttaa ttgccaaaag
    19681 aacaccactt tctatttcaa attttcttta acaactttaa gttgttgttt aatactcaca
    19741 tcaattgtct tgtgattggc aaaaatgaca aaaccaccaa tgagactttc atcgatttgt
    19801 tcttttacac tccttacttt cagagacatt tttttctcaa tcaaagggag caaacgagtc
    19861 ttctgttcat cagttagagg atgagcagac gtaatcgtca cttcaaatcg atttgtttct
    19921 ttttcaagtc ggttcaagca atctacaagc acatcataaa aaagatttgc tctgtgattg
    19981 taggccagaa cctggataaa gttttgtaat aaaggcgaca cagaatcttg gaaaaaagca
    20041 attgtttttt ccttatccga ctcgtctact gccacttgtt ttaaaaaaga aggcagacct
    20101 gttttttcaa caacttgctt gatttgagtc aagtctgaaa agatacggtc ttcttctcct
    20161 ttttcaagta ccaattggac aaaaggcatg ctgtattttt caattacctt tactgttttc
    20221 ttgtccatta agcttctcct agctgatcga tatactgatc aatgagtgct ttatgggcat
    20281 gactgtcaag gttttgtgag atgattttac cagctaagct gatggtcaaa tctgcgacct
    20341 cacccttaac actctgtaaa gcttctactt tattttgagc aatttcttga ttggcttttt
    20401 cttttaagtg tcctgcttct agtttagcat ctgctaagat attagcctta ctttgctcag
    20461 ctgtttcctt tgcattttca atgattgtct tagcttcttt acggctacca gccaattcat
    20521 cttcgcgttt ttgagccaat acttctgctt tttgacgggc ttcttcagct ctgtcaatat
    20581 ctgaagcaat tttttcagct ctttcttcga aaatgcctgt aatattagac catgcaaatt
    20641 ttttaatcaa gactagcaaa agaataaaag agccagtgat taaaataaaa ttaccaatta
    20701 attcacctac tgttacgtgc atcagttact cctttctatt cctcttcaat attaatctta
    20761 ttccctaaat acacagatgt caaaagagta aaaacataag cttggatgca ggaaataaag
    20821 acagaaaatg cagtccaagc caaattagct ccaaaggcta ctggatacca ataaatagct
    20881 tggtgggaaa gaagaagtaa caaactcgtc atgacctctc ctgcaaagat attcccaaaa
    20941 atccgcaaag ccaaagataa gaagtttgta aattcttcca agatattcat cggtatgaca
    21001 aaaacaggac tcataaaaga ttttatactt tttttaaatc cacgacgacg aacgctttct
    21061 atatgtgtca acaaaatgac aagaaaagat aaggttaagt catactgtaa attagcggtt
    21121 ggcgaactcc accagttagt cccatcgatc gtttgaatct ttgtcattaa gccaaggtta
    21181 ttggcaatca ccatgaaaag gaataaacaa aggaaaaaga gtgagtaatc tttcatgtag
    21241 cgcgaaccaa tgttaggttc tgtaaatcca ataacaaagt catagacata ctcaagtaca
    21301 ttttgctttc ctttgggttt caaggtcata ttgcggcttg cccaataaat aaagacaaaa
    21361 ataactccca caatcaacaa agtcatggct aacatagtca gattgaagat aacaggacca
    21421 atagagatga ttggattaat actttcttcc atctaaaatc ctcccttttc ttctcgttcc
    21481 atgtatttat ttgataataa atgagaagac aagagttaca aagaaagttc cttcaataaa
    21541 ggcaacacct aaaaacatca aactacgaaa ctcagaaagc atatctggtt ggcgtgctac
    21601 tgatttaaac agtccattca tcaataaacc ttcaccgaca gatacgccca tacaggcaat
    21661 acataagcct aaaaatgtta aattcataac aaattctcct ttaaaataaa atttacttac
    21721 ttagtgtagt cctaaaattt aaatttgtca acttttaacc gctattggaa gcgtttaata
    21781 aaaattattt ccattttgct aaatttcaag ttcaagttag ccatccagaa gtcttctctg
    21841 ggtgacttgt agtccaagca ttttttagga tagttgttaa tccacttttc gatgaatgcg
    21901 acttctttgg gagtcatttt cttggttccc ttaggtaacc atctacgaat gagcctgttg
    21961 tgattctcat tagttcccct ttcccaagag gcatagggat gtgcataata aatgtgctcc
    22021 tcagaaaata cattagacaa gcgattgaat tccgttccat tatctgccgt gatggaaaga
    22081 atcttgtgtt gttttaagat gagttttaga gcctgattga ccacctcagc acttttattt
    22141 ggaatcaatc ggatgatctg atgtctactc tttcgatccg tcaagacaag caagcagtag
    22201 tttttcgatc tcgtaagtag aaccgtatca atctcataat gcccattctc caagcgaaga
    22261 ttgatagctt caggccgctg tgcgatggat tgaccagcag gtttaaagtt ggtgctagcc
    22321 tgtttcttaa gcacttttcc ttttctaggg taaagcagat cctgcttgct taaccccaat
    22381 tttccatgat gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc
    22441 accatcattt caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc
    22501 ttggtcaagc ttgatttctt gaccgagcgc ttgcgattgt tttcataaga ctgttgagca
    22561 tagtcggcag aataaacctc tttgaagcgc ccttttccaa gacattttcg gactgtccta
    22621 cgcttgattt tagtgtggat agtttgagga gcttttccaa gcagagaggc aatttctcta
    22681 tttgatttcc ctttgttcgc cttttgtagt ataatggttt tgcatctctg tgcctttctt
    22741 gtgtttgtgg ttgaacaaca agtataacac agaggtgttt tcttatgcct acaagagctt
    22801 tcattatttc cattttcttt cggatttcac tctattctga aaaacttgtg tatatttact
    22861 gaagctagca agtcttacct gtaaatttaa tgaaagcaac acaaaatccg agaggggaat
    22921 ctcggattaa tgtcaattta aaaagataga gagtttttag tttaaataaa ttgttttaaa
    22981 atatcaacaa catcacttct tttcttaacc tgataagcct tgattcctaa ttttggagct
    23041 acgattatat tgtcctcaat atcgtctaga aagacacaat ttctaggtta taactggtat
    23101 ttatcgatag ttactcatat atatcagtcc acctccatac tcaccttaca gattcttttg
    23161 ataataatat ctttgcctaa tgtagagata gtcttgcaaa gaaaaaactt ccttgtagcc
    23221 atgtttctga taaaagtccg gtgcctggaa ctggtaagta ttgacaaagg caaaacaaca
    23281 atttcgattc ttagcttcac tttctgcctg ttgcaatagt tttgaaccga ttccttgccc
    23341 tcgcagttcc tcttttacaa acaaatactc gatttctagc caatttccaa aagtctctgc
    23401 tatcaaacct gccaggagat tgcccttttc atcttcgaca taaagattaa gtggctcact
    23461 ttcagcctct tctctttttg aacggttata aacacgaatc agattcccta tttcttgcga
    23521 tttatgtgat tccttatttt ccaatctaaa gtataatgaa atgaaataaa acatgcgcaa
    23581 atcgatt
//
LOCUS       DAVKZB010000021        22451 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKZB010000021 DAVKZB010000000
VERSION     DAVKZB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.021"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            165..944
                     /gene="rpsB"
                     /locus_tag="VXA80_000750"
     CDS             165..944
                     /gene="rpsB"
                     /locus_tag="VXA80_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV0353285.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            1023..2063
                     /locus_tag="VXA80_000751"
     CDS             1023..2063
                     /locus_tag="VXA80_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV0353286.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKSQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            2309..2649
                     /gene="tnpB"
                     /locus_tag="VXA80_000752"
                     /pseudo
     CDS             2309..2649
                     /gene="tnpB"
                     /locus_tag="VXA80_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2682..2990)
                     /locus_tag="VXA80_000753"
                     /pseudo
     CDS             complement(2682..2990)
                     /locus_tag="VXA80_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2981..3379
                     /locus_tag="VXA80_000754"
     CDS             2981..3379
                     /locus_tag="VXA80_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0353287.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(3576..4496)
                     /gene="cysK"
                     /locus_tag="VXA80_000755"
     CDS             complement(3576..4496)
                     /gene="cysK"
                     /locus_tag="VXA80_000755"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV0353288.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGATDVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(4781..5416)
                     /locus_tag="VXA80_000756"
     CDS             complement(4781..5416)
                     /locus_tag="VXA80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV0353289.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            5472..6770
                     /locus_tag="VXA80_000757"
     CDS             5472..6770
                     /locus_tag="VXA80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0353290.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            6767..7429
                     /locus_tag="VXA80_000758"
     CDS             6767..7429
                     /locus_tag="VXA80_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV0353291.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            7509..8057
                     /gene="raiA"
                     /locus_tag="VXA80_000759"
     CDS             7509..8057
                     /gene="raiA"
                     /locus_tag="VXA80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV0353292.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            8193..10166
                     /locus_tag="VXA80_000760"
     CDS             8193..10166
                     /locus_tag="VXA80_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV0353293.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            10163..10615
                     /gene="rplI"
                     /locus_tag="VXA80_000761"
     CDS             10163..10615
                     /gene="rplI"
                     /locus_tag="VXA80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV0353294.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            10659..12011
                     /gene="dnaB"
                     /locus_tag="VXA80_000762"
     CDS             10659..12011
                     /gene="dnaB"
                     /locus_tag="VXA80_000762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV0353295.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            12013..12279
                     /locus_tag="VXA80_000763"
     CDS             12013..12279
                     /locus_tag="VXA80_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353296.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            12368..13714
                     /gene="cbpD"
                     /locus_tag="VXA80_000764"
     CDS             12368..13714
                     /gene="cbpD"
                     /locus_tag="VXA80_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV0353297.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVNNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      13797..13899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13970..14260
                     /locus_tag="VXA80_000765"
     CDS             13970..14260
                     /locus_tag="VXA80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV0353298.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            14223..14981
                     /locus_tag="VXA80_000766"
     CDS             14223..14981
                     /locus_tag="VXA80_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353299.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            15019..16026
                     /locus_tag="VXA80_000767"
     CDS             15019..16026
                     /locus_tag="VXA80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0353300.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            16026..16754
                     /locus_tag="VXA80_000768"
     CDS             16026..16754
                     /locus_tag="VXA80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353301.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLHLTTLIITHSIEEALNISDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            16873..17331
                     /locus_tag="VXA80_000769"
     CDS             16873..17331
                     /locus_tag="VXA80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV0353302.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            17333..19765
                     /locus_tag="VXA80_000770"
     CDS             17333..19765
                     /locus_tag="VXA80_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0353303.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDETVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            20407..21060
                     /gene="rr06"
                     /locus_tag="VXA80_000771"
     CDS             20407..21060
                     /gene="rr06"
                     /locus_tag="VXA80_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV0353304.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            21057..22388
                     /gene="hk06"
                     /locus_tag="VXA80_000772"
     CDS             21057..22388
                     /gene="hk06"
                     /locus_tag="VXA80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV0353305.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
BASE COUNT         6955 a         3792 c         5056 g         6648 t
ORIGIN      
        1 aaaattatat caaaatagct tgaaaatatt ggaaaagtat ggtaaaatga aaattgtcgt
       61 gtgaacgata atactcattc ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg
      121 agttgaagtc aagaagagga aaaaaacaaa aaggagaaat actcatggca gtaatttcaa
      181 tgaaacaact tcttgaggct ggtgtacact ttggtcacca aactcgtcgc tggaatccta
      241 agatggctaa gtacatcttt actgaacgta acggaatcca cgttatcgac ttgcaacaaa
      301 ctgtaaaata cgctgaccaa gcatacgact tcatgcgtga tgcagcagct aacgatgcag
      361 ttgtattgtt cgttggtact aagaaacaag cagctgatgc agttgctgaa gaagcagtac
      421 gttcaggtca atacttcatc aaccaccgtt ggttgggtgg aactcttaca aactggggaa
      481 caatccaaaa acgtatcgct cgtttgaaag aaattaaacg tatggaagaa gatggaactt
      541 tcgaagttct tcctaagaaa gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa
      601 aattcttggg cggtatcgaa gatatgcctc gtatcccaga tgtgatgtac gtagttgacc
      661 cacataaaga gcaaatcgct gttaaagaag ctaaaaaatt gggaatccca gttgtagcga
      721 tggttgacac caatactgat ccagatgata tcgatgtaat catcccagct aacgatgacg
      781 ctatccgtgc tgttaaattg atcacagcta aattggctga cgctattatc gaaggacgtc
      841 aaggtgagga tgcagtagca gttgaagcag aatttgcagc tttagaaact caagcagatt
      901 caattgaaga aatcgttgaa gttgtagaag gcgacaacgc ttaatttata caaatagtaa
      961 ttacctagga gggcggggct tagcccggct ctcctatttt caaaaaatat aggagaatta
     1021 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
     1081 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
     1141 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
     1201 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aacgctgaaa
     1261 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
     1321 ttgctgaagg aaaacctgct aacaacgaag aagctcttgc tttgataatg ccttcaggtg
     1381 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
     1441 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
     1501 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
     1561 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
     1621 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
     1681 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa tctcaattaa
     1741 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggca
     1801 aaccagaaaa aatctgggac aaaattattc caggtaaaat ggatcgcttc atgcttgata
     1861 acactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
     1921 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
     1981 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
     2041 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
     2101 gggcttagat ttttcctgaa attgtttttt ccccagtctc actcgattag aataattaaa
     2161 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
     2221 aagcgcgtca taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa
     2281 ataattatcg gaggaaaggt attgtactat gatgattcaa ctaagtgatt taggtcaagt
     2341 tcacattgtt tgtggcaaga cagatatgag gcagggaata gactcattag cctatgtagt
     2401 taaaacccac tttgaattgg attctttctc cggtcaagtc tttctctttt gtggtggacg
     2461 taaagaccgc tttaaagccc tttactggga tggtcaagga ttttggctac tatataaacg
     2521 ctttgagaac gtcagactga cttggcccag tacagaaaag gatgtcaaag ctctcacacc
     2581 tgaacaagta gactggctta tgaagggctt ttctatcact ccaaaaataa atccatcaga
     2641 aagtcgtgat ttctattgaa atgaggactt tctttttata ctcatctgct ttcaaaaagc
     2701 attctagtcc atctccgatt aacgatggac tttatcacat ccttctccag tccttgtata
     2761 acatcttgga gttgattcat gacatcttcc aaagtttaaa aggctttatt cttaaatcca
     2821 cgtttacgaa tctctttcca cacttgttca atggggttca tctctggtgt gtatggagga
     2881 ataaatgcaa agccaatatt agtcggaatc tttaaggtac ttgatttatg ccatatagca
     2941 ttgtccataa cgagtaaaag ataatcatct ggataagctt gtgaaatctc ctattcctaa
     3001 agccccttta gcgcataact ttggctcagc ttctattatc gctcacacca tccatcagaa
     3061 gtttaatctg aaggtaccca attatcgcca agaagaagat tgggctagga tgggtttacc
     3121 aatcacacgt aaggaaatct ctaatcggca tatcaagacg agtcaatact atttggagcc
     3181 cctttataac ctcttgcgag agagactatt gactcagccc ttacttcatg cggatgaaac
     3241 ttcttatagg gtgctagaga gtgatagtca gctgacttac tattggactt ttttgtctgg
     3301 gaaagctgag aaacaaggga ttacgcttta ccatcatgtc ctaatcgatt tgttcatatc
     3361 ttatttcaat ccattataaa tagtgagaaa tatctatcct atcttctaga atgtcttcca
     3421 aacgtggaaa ctctcgtaaa caaagaggtt ttagaggcct atttaccatg gactaaagtt
     3481 gtacaagaaa agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctctagt
     3541 ctggagattt ttcaatatac ttcgttattg gacggttaca attcataaag tgctgtagag
     3601 agataacgtt caccgttatc tggtgctagg gcaaggactt ttttacctgt acctaatttt
     3661 ttggcaacct cgatggctcc gtagatagct gcagctgagg aaatccctac aaggaagcct
     3721 tcttttccac caatttcacg tccgagtgca agagcgtcat ctgatgttac acgaacgata
     3781 ccatcatagg ctttagtatc aagtgtatca ggaataaatc cagctgagat accttgaatt
     3841 ttgtgaggac caggtttttc accagataga atagcagatt catctgcttc tactgcaaaa
     3901 acttgaatgt tagaattttc tgatttgagt gcatgagaaa caccagaaat cgttccaccg
     3961 gtacctactc cagcaacaaa ggcatctaat ccatctttac cgaaagcagc tagtatctca
     4021 gctcctgttg ttctttcgtg tacttctgga ttagctggat tgtcaaattg aagaggaagg
     4081 aaaccatcac gttcagcagc gatttcttga gccttagcaa tagcaccttt cattccctcg
     4141 ctaccaggag ttaggacgag ttcagcacca taagcttgga taattttacg tcgttctaca
     4201 ctcatagttt caggcataac gatgacgact ttataccctt tagcagcacc tacccatgaa
     4261 agtccaatac cagtgtttcc acttgttgct tcaacaatag tagaaccagg tttcagaata
     4321 ccatcttgtt cagctttttc aatcatgcta agggcaatac ggtcttttac agatgaacca
     4381 ggattaaatg cttcaagctt tatatagacg tctgtagcac cttctggcac gatgttgtta
     4441 agtttaacaa tcggtgtttg accgattaat tcagtaatgt tgttataaat agacatatga
     4501 atacctcttt gtataagata tctttagtat aacgttatct ataccatttg taaaatatag
     4561 aaatcctatc gaagcgatag gatttctata tatcatagat caaagtcatt aattttcaag
     4621 tgattgcgtt cagctaattc tagtaaatag gtgtgaagga aaacgatatc aggtttcgtt
     4681 tgaattgctt cattttctag tatattttgg tacgcatctt tttattttgc tcgctaaagt
     4741 agttgtgaga atgtcaacga aacgctgtat tattcattgt ttacactaag tttacaggaa
     4801 cttcaacctc tcgtaaacct tggtcagtta aagtgacttt tccattaaaa aactccacaa
     4861 gtgcagcttt aatagtttct ttttcttctt tatcaacata aatcatcgta tcgacttgat
     4921 ctgtaaagtt tgtatccagc tccatgagat catgttcttt aaggaagtta ctgtactctt
     4981 ggtactgagc ataagacatt tgaatagcaa tgccagcctg ttcttttatt tcaataatac
     5041 caatttcttt gacagctaag gcgacactgc cggcgtaagc acgaattagt cctccagcgc
     5101 ctagtttaat accaccaaag tagcgtgtca cgaccacaca gacattggtg agattgtgat
     5161 tttctagtac cccaagcatg ggaacaccag cagtaccact aggctcacca tcatcacttg
     5221 tacgtttaat ttcactacgt tctccaataa taaaggcaga gcagttatgt gtcgctttgt
     5281 agtgttcttt tttgatggca gtaatgaagt cacgagcctc ttcttcgcta taaacacgct
     5341 tggcatggca gataaagcga gattttttga tttcttcttg gacttggccg tcctctttaa
     5401 ttgtcctaaa ttccatgatt taattgtact aaaaaatcat ctaaagtgct agtttttacg
     5461 aataaagaag tatgaaagta aatttagatt atctcggtcg tttatttact gagaatgaat
     5521 taacagaaga agaacgtcag ttggcggaga aacttccagc aatgagaaag gagaagggga
     5581 aacttttctg tcaacgttgt aatagtacta ttctagaaga atggtatttg cccatcggtg
     5641 cttactattg tcgagagtgc ttgctgatga agcgagtcag aagtgatcaa actttatact
     5701 attttccgca ggaggatttt ccgaagcaag atgttctcaa atggcgcggc caattaactc
     5761 cttttcaaga gaaggtgtca gagggactgc ttcaagcagt agacaagcaa aagccaacct
     5821 tagttcatgc ggtaacagga gctggaaaga cagaaatgat ttatcaagta gtggctaaag
     5881 tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg catagatgtt tgtttggagc
     5941 tgtacaagcg cctgcaacag gatttttctt gcgggatagc tttgctacat ggagaatcgg
     6001 aaccttattt tcgaacacca ctagttgttg caacaaccca tcagttattg aagttttatc
     6061 aagcttttga tttgctgata gtggatgaag tagatgcttt tccttatgtt gataatccca
     6121 cgctttacca cgctgtcaag aatagtgtaa aggagaatgg attgagaatc tttttaacag
     6181 cgacttcgac caatgagtta gataaaaagg tccgtttagg agaactaaaa agactgagtt
     6241 taccgagacg gtttcatgga aatccgttga ttattccaaa accaatttgg ttatcggatt
     6301 ttaatcgcta cttagacaag aatcgtttgt caccaaagtt aaagtcctat attgagaagc
     6361 agagaaagac agcttatccg ttactcattt ttgcttcaga aattaagaaa ggggagcagt
     6421 tagcagaaat cttacaggag caatttccaa atgagaaaat tggctttgta tcttctgtaa
     6481 cagaggatcg attagagcaa gtacaagctt ttcgagatgg agaactgaca atacttatca
     6541 gtacgacaat cttggagcgt ggagttacct tcccttgtgt ggatgttttc gtagtagagg
     6601 ccaatcatcg tttgtttacc aagtctagtt tgattcagat tggtggacga gttggacgaa
     6661 gcatggatag accgacagga gatttgcttt tcttccatga tgggttaaat gcttcaatca
     6721 agaaggcgat taaggaaatt cagatgatga ataaggaggc tggtctatga agtgcttgtt
     6781 atgtgggcag actatgaaga ctgttttaac ttttagtagt ctcttacttc tgaggaatga
     6841 tgactcttgt ctttgttcag actgtgattc tacttttgaa agaattgggg aagagaactg
     6901 tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa gattgtcaac tttggtgtaa
     6961 agagggagtt gaagtcagtc atagagcgat ttttacttac aatcaagcta tgaaggattt
     7021 tttcagtcgg tataagtttg atggagactt cctgttaaga aaagttttcg cttcattttt
     7081 aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt gtaattcccc taagtcctga
     7141 tagatatgct aatagaggat ttaatcaggt tgagggcttg gtagaggcag caggctttga
     7201 gtatctggat ttattagaga aaagagaaga gagagccagt tcttctaaaa atcgttcaga
     7261 gcgcttgggg acagaacttc ctttctttat taaaagtgga gtcactattc ctaaaaaaat
     7321 cctacttata gatgatatct atactacagg agcaactata aatcgtgtta agaaactgtt
     7381 ggaagaagct ggtgctaagg atgtaaaaac attttccctt gtaagatgag gaaaaaattt
     7441 gcaaaaatag aatgaaagcg ttataataaa atcataaaaa caaaaatgtt tagaaagaag
     7501 gtactcatat gattaaatat agtatccgtg gtgaaaacct agaagtaaca gaagcaattc
     7561 gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta cttccaacca gaacaagagt
     7621 tggatgcccg aattaactta aaagtttatc gtgaaaaaac ggctaaagtg gaagtaacga
     7681 ttccgcttgg atctattact ctccgcgcag aagatgtatc tcaagatatg tatggttcaa
     7741 ttgaccttgt aactgataaa attgaacgtc agattcgtaa aaataaaaca aaaatcgagc
     7801 gtaaaaataa aaataaggta gcaactggtc aattatttac agatgctttg gtggaagatt
     7861 caaatgttgt ccagtctaaa gttgttcgtt caaaacaaat tgatttaaaa ccaatggatt
     7921 tggaagaagc aattctacaa atggatttat tggggcatga tttctttatc tatgtggatg
     7981 ttgaagatca gacaaccaat gtgatttatc gtcgtgagga tggcgaaatt ggtttgttag
     8041 aggttaaaga atcttaattt caatatgtgt aaaggtaggt ttactgaatt gtaaactacc
     8101 tttttcttat aattgataga attactgatt acacttttaa aaagtcgctt tttgaggggt
     8161 attatggtat aatgggtgcc aagaggtttt gaatgaaaaa attttatgta agtccaattt
     8221 ttcctattct agtaggattg attgcgtttg gagtcttatc cactttcatt atttttgtta
     8281 ataataatct gttgacggtt ttaattttgt ttctttttgt aggaggctat gtttttttat
     8341 ttaagaaact gagagtgcat tatacaagga gtgatgtaga acagatacag tatgtaaacc
     8401 accaagcgga agaaagtttg acatctctat tggaacaaat gcctgtaggt gttatgaaat
     8461 tgaatttatc ttctggagag gttgagtggt ttaatcccta tgctgaattg attttgacca
     8521 aggaagatgg tgattttgat ttagaagctg ttcaaacgat tatcaaggct tcagtaggaa
     8581 atccgtctac ttatgccaag cttggtgaga agcgttatgc tgttcatatg gatgcttctt
     8641 ccggtgtttt gtattttgta gatgtatcca gggaacaagc cataacagat gaattggtaa
     8701 caagtcgacc agtgattggg attgtctctg tggataatta tgatgatttg gaggatgcaa
     8761 cttctgagtc agatattagt caaatcaata gttttgtagc taattttata tcagagtttt
     8821 cagaaaaata catgatgttt tctcgtcggg taagtatgga tcgattttat ctatttactg
     8881 actacacggt gcttgagggc ttgatgaatg ataaattttc tgttattgat gctttcagag
     8941 aagagtcgaa acagagacag ttgcccttga ccttaagtat gggattttct tatggcgatg
     9001 gaaatcatga tgagataggg aaagttgctt tgctcaattt gaacttggct gaagtacgtg
     9061 gtggcgacca ggtggttgtt aaggaaaacg acgaaacgaa aaatccagtt tattttggtg
     9121 gtgggtctgc tgcttcaatc aagcgtacac ggactcgtac gcgcgctatg atgacagcta
     9181 tttcagataa gattcggagt gtagatcatg tttttgtagt cggtcacaaa aatttagaca
     9241 tggatgcttt gggctctgct gtaggtatgc agttgttcgc cagcaatgtg attgaaaata
     9301 gctatgctct ttatgatgaa gaacaaatgt ctccagatat tgaacgagct gtttcattca
     9361 tagaaaaaga aggagttacg aagttgttgt ctgttaagga tgcaatgggg atggtgacca
     9421 atcgttcttt gttgattctt gtagaccatt caaagacagc cttaacatta tcaaaagaat
     9481 tttatgattt atttacccaa accattgtta ttgaccacca tagaagggat caggattttc
     9541 cagataatgc ggttattact tatatcgaaa gtggtgcaag tagtgccagt gagttggtaa
     9601 cggaattgat tcagttccag aattctaaga aaaatcgttt gagtcgtatg caagcaagtg
     9661 tcttgatggc tggtatgatg ttggatacta aaaatttcac ctcgcgagta actagtcgga
     9721 catttgatgt tgctagctat ctcagaacgc gcggaagtga tagtattgct atccaggaaa
     9781 tcgctgcgac agattttgaa gaatatcgtg aggtcaatga acttatttta caggggcgta
     9841 aattaggttc agatgtacta atagcagagg ctaaggacac gaaatgctat gatacagttg
     9901 ttattagtaa ggcagcagat gccatgttag ccatgtcagg tattgaagcg agttttgttc
     9961 ttgcgaagaa tacacaagga tttatctcta tctcagctcg aagtcgtagt aaactgaatg
    10021 tacaacggat tatggaagag ttaggcggtg gaggccactt taatttggca gcagctcaaa
    10081 ttaaagatgt aaccttgtca gaagcaggtg aaaaactgac agaaattgta ttaaatgaaa
    10141 taaaggaaaa ggagaaagaa gaatgaaagt aatcttttta gcagatgtta aaggaaaagg
    10201 taaaaaaggc gaaattaagg aagtaccaac agggtatgcg caaaactttc ttatcaaaaa
    10261 gaatctagcc aaagaagcga ctgctcaagc tgtaggtgaa cttcgtggta aacaaaaatc
    10321 ggaagaaaaa gctcacgctg agatgattgc agaaggaaaa gcaattaaag cacaacttga
    10381 agcagaagaa actgttgtag aatttgttga aaaagttggt ccagatggtc gtacctttgg
    10441 ttctattacc aataagaaga ttgcagaaga attgcaaaag caatttggaa ttaagattga
    10501 taaacgtcat attcaagtac aagctccgat tcgagcggtt ggtttgattg atgtgccagt
    10561 gaaaatctat caagatatca caagtgtaat caatcttcgt gtgaaagaag gataagttta
    10621 cactttcttg acaagattgt aaaaggaagg gaagtctgat ggcagaagta gaagagttac
    10681 gagtacaacc tcaagatatc ttagctgagc aatccgtttt aggggctatc tttattgatg
    10741 agagtaaact tgtttttgtg cgagaataca ttgagtctcg ggactttttt aagtatgccc
    10801 atcgtttgat tttccaagcc atggtcgatt tatccgatcg tggtgatgcc atagatgcaa
    10861 caacggttcg tactatcctt gataatcaag gtgatttaca gaatattggt ggcttgtctt
    10921 acttggttga gattgttaat tctgtgccaa cttctgctaa tgcggagtat tatgctaaga
    10981 ttgttgcaga aaaagcaatg ctacgtcgtt taattgccaa gttgacagag tctgtcaacc
    11041 aagcttacga agcgtcacaa ccagctgatg aaattattgc tcaggcagaa aaagggttga
    11101 ttgatgtcag tgaaaatgca aatcgaagcg ggtttaagaa cattcgagat gtgttgaatc
    11161 tcaactttgg aaatctggaa gctcgctcgc aacaaacgac cgatattaca ggtattgcga
    11221 caggttatcg tgatttggat catatgacaa caggacttca tgaggaggag ttgattatct
    11281 tagcagctcg tccagcagtt ggtaagacag catttgcctt gaatatcgct cagaacattg
    11341 ggactaagtt ggacaaaacg gttgctattt tttcactcga aatgggtgcg gaaagcttgg
    11401 tagatcgtat gttagctgca gaaggcttgg tggagtcaca ttctatccgt acagggcaat
    11461 tgacagatga ggagtggcaa aaatatacta ttgctcaggg taatctagct aacgccagta
    11521 tctatatcga tgatacgcca ggtattcgga ttacagagat tcgttctcgt tctcgtaaat
    11581 tggctcaaga aactggaaat cttggtttga ttttgataga ctatttgcaa cttatcacgg
    11641 gaactggtcg agaaaatcgt caacaagaag tttctgaaat ttctcgtcag ttgaaaatac
    11701 tagccaagga attgaaggtt ccagtaatcg ctctgagtca gctttctcgt ggtgtagaac
    11761 aacgtcagga caagagaccg gtcttgtctg atattcgtga atctgggtct attgagcagg
    11821 acgctgatat cgtagctttt ctctatcgcg atgactacta tgaacgtggt ggtgaagaag
    11881 aggagggtat cccaaataat aaggtggaag ttattatcga gaaaaaccgt agtggagctc
    11941 gtggaacagt ggaattgatt ttccaaaaag aatacaataa attttcaagt atctcaaaga
    12001 gggaggcata agatgtcaga tgcatttaca gatgtagcca agatgaaaaa aatcaaagaa
    12061 gaaattaagg cacatgaggg acaagtcgta gaaatgactt tggagaatgg tcgtaagcgc
    12121 caaaaaaata gattgggtaa gctaattgaa gtttatccat ctctatttat tgtggagttt
    12181 ggggatgtgg aaggagataa acaagttaat gtttacgttg aatcctttac ttactcagat
    12241 attcttacag aaaagaattt gattcattat cttgactaaa gtgagaaatt ttctcacttt
    12301 ttcttttttc tccgaataat ttaggtgaag gcaatcatcg ctttatatta ttttttaagg
    12361 aggaagaatg aaaattttac cgtttatagc aagaggaaca agttattact tgaagatgtc
    12421 agttaaaaag cttgttcctt ttttagtagt aggattgatg ctagcagctg gtgatagtgt
    12481 ctatgcctat tccagaggaa atggatcgat tgcgcgtggg gatgattatc ctgcttatta
    12541 taaaaatggg agccaggaga ttgatcagtg gcgcatgtat tctcgtcaat gtacttcttt
    12601 tgtagccttt cgtttgagta atgtcaatgg ttttgaaatt ccggcagctt atggaaatgc
    12661 gaatgaatgg ggacatcgtg ctcgtcggga aggttatcgt gtaaataata caccgacgat
    12721 tggttccatt acttggtcta ctgcaggaac ttatggtcat gttgcctggg tgtcaaatgt
    12781 aatgggagat cagattgaga ttgaggaata taactatggt tatacagaat cctataataa
    12841 acgaattata aaagcaaaca cgatgacagg atttattcat tttaaagatt tggatggtgg
    12901 cagtgttggg aatagtcaat cctcaacttc aacaggcgga actcattatt ttaagaccaa
    12961 gtctgctatt aaaactgaac ccctagttag tgcaactgtg attgattact attatcctgg
    13021 agagaaggtt cattatgatc aaattctcga aaaagacggc tacaagtggt tgagttatac
    13081 tgcctataat ggaagctatc gttatgttca attggaggct gtgaataaaa atcctctagg
    13141 taattctgtt ctttcttcaa caggtggaac tcattatttt aagaccaagt ctgctatcaa
    13201 aactgaaccc ctagttagtg caactgtgat tgattactat tatcctgggg agaaggttca
    13261 ttatgatcag atacttgaaa aagacggcta caagtggttg agttatacgg cttataacgg
    13321 aagtcgtcgc tatatacagc tagagggagt gacttcttca caaaattatc agaatcaatc
    13381 aggaaatatc tctagctatg gatccaataa tagttcaact gtcggttgga agaaaataaa
    13441 tggtagttgg tatcatttca aatcaaatgg ttctaaatca acaggatggc tgaaagacgg
    13501 ttctagctgg tattatttga aattatctgg tgaaatgcag acaggatggt taaaggagaa
    13561 tggctcgtgg tattatctgg gtagttcagg ggcaatgaaa acaggctggt accaggtctc
    13621 tggtaagtgg tattattctt actcttcagg cgccttagct gttaatacga cggtggatgg
    13681 ctacagagta aacagtgatg gagaacgagt atagaaaatt ggagtaggag aaatttcctg
    13741 ctcttttctg taagcagttt ccttgacatt tttcttattt tgcgctatca tatatacata
    13801 taatatatgg gagtctgtgt acagtctgag aggaagtgtt aaacttcgac cgcacctgat
    13861 ctgggtaatg ccagcggagg gaacgatact tagtctaatt ttgcaccttt tccatgtatg
    13921 gtaaaggttt ttcttttttt aaaaaggaaa acgagaagag gaggttctta tgaaagcaag
    13981 cattgccttg caagttttac ccctagtaca ggggattgat cggatagctg ttattgatca
    14041 ggtcattgct tatctgcaaa ctcaagaagt gacgatggta gtgacaccat ttgaaacggt
    14101 cttggaaggg gagtttgatg agcttatgcg cattctaaaa gaagcgctgg aagtggcagg
    14161 gcaggaggca gacaatgtct ttgccaatgt caaaataaat gtaggagaga ttttaagtat
    14221 tgatgagaaa cttgagaagt atactgagac gacacattag tctattgggc tttctcggag
    14281 tattgtcaat ctggcagtta gcaggttttc ttaaactgct ccccaagttt atcctgccga
    14341 cacctcttga aattctccag ccctttgttc gtgacagaga atttctctgg caccatagct
    14401 gggcgacctt gagagtggct ttactggggc tgattttggg agttttgatt gcctgtctta
    14461 tggctgtgct catggatagt ttgacttggc tcaatgacct gatttaccct atgatggtgg
    14521 tcattcagac cattccgacc attgccatag ctcctatcct ggtcttgtgg ctaggttatg
    14581 ggattttgcc caagattgtc ttgattatct taacaacaac ctttcccatc atcgttagta
    14641 ttttggacgg ttttaggcat tgtgacaagg atatgctgac cttgtttagt ctgatgcggg
    14701 ccaagccttg gcaaatcctg tggcatttta aaatcccggt tagcctgcct tacttttatg
    14761 caggtctgag ggtcagtgtc tcctacgcct ttatcacaac tgtggtatct gagtggttgg
    14821 gaggttttga aggtcttggt gtttatatga ttcagtctaa aaaactgttt cagtatgata
    14881 ccatgtttgc cattattatt ctggtgtcga ttatcagtct tttgggtatg aagctggtcg
    14941 atatcagtga aaaatatgtg attaaatgga aacgtactta aaatagagcg tttttgaaaa
    15001 agaaaagagg aaatcaaaat gaagaaaaca tggaaagtgt ttttaacgct tgtaacagct
    15061 cttgtagctg ttgtgcttgt ggcctgtggt caaggaactg cttctaaaga caacaaagag
    15121 gcagaactta agaaggttga ctttatccta gactggacac caaataccaa ccacacaggg
    15181 ctttatgttg ccaaggaaaa aggttatttc aaagaagctg gagtggatgt tgatttgaaa
    15241 ttgccaccag aagaaagttc ttctgacttg gttatcaacg gaaaggcacc atttgcagtg
    15301 tatttccaag actacatggc taagaaattg gaaaaaggag caggaatcac tgccgttgca
    15361 gctattgttg aacacaatac atcaggaatc atctctcgta aatctgataa tgtaagcagt
    15421 ccaaaagact tggttggtaa gaaatatggg acatggaatg acccaactga acttgctatg
    15481 ttgaaaacct tggtagaatc tcaaggtgga gactttgaga aggttgaaaa agtaccaaat
    15541 aacgactcaa actcaatcac accgattgcc aatggcgtct ttgatactgc ttggatttac
    15601 tacggttggg atggtatcct tgctaaatct caaggtgtag atgctaactt catgtacttg
    15661 aaagactatg tcaaggagtt tgactactat tcaccagtta tcatcgcaaa caacgactat
    15721 ctgaaagata acaaagaaga agctcgcaaa gtcatccaag ccatcaaaaa aggctaccaa
    15781 tatgccatgg aacatccaga agaagctgca gatattctca tcaagaatgc acctgaactc
    15841 aaggaaaaac gtgactttgt catcgaatct caaaaatact tgtcaaaaga atacgcaagc
    15901 gacaaggaaa aatggggtca atttgacgca gctcgctgga atgctttcta caaatgggat
    15961 aaagaaaatg gtatccttaa agaagacttg acagacaaag gcttcaccaa cgaatttgtg
    16021 aaataatgac agaaattaga ctagagcacg tcagttatgc ctatggtcag gagaggattt
    16081 tagaggatat caacctacag gtgacttcag gcgaagtggt ttccatccta ggcccaagtg
    16141 gtgttggaaa gaccaccctc tttaatctaa tcgctgggat tttagaagtt cagtcaggga
    16201 gaattgtcct tgatggtgaa gaaaatccca aggggcgcgt gagttatatg ttgcaaaagg
    16261 atctgctctt ggagcacaag acggtgcttg gaaatatcat tctgcccctc ttgattcaaa
    16321 aggtggataa ggcagaagct atttcccgag cggataaaat tcttgcgacc ttccagctga
    16381 cagctgtaag agacaagtat cctcatgaac ttagcggtgg gatgcgccag cgtgtagcct
    16441 tactccggac ctaccttttt gggcacaagc tctttctctt agatgaggcc tttagcgcct
    16501 tggatgagat gacaaagatg gaactccacg cttggtatct tgagattcac aagcagttgc
    16561 atctaacaac cctgatcatc acgcatagta ttgaggaggc cctcaatatc agcgaccgca
    16621 tctatatctt gaaaaatcgc cctgggcaga ttgtttcaga aattaaacta gattggtctg
    16681 aagatgagga caaggaagtc caaaagattg cctacaaacg tcaaatcttg gcagaattag
    16741 gcttagataa gtagaaaaat agggagttgg tgaagattat cctttaccag cgcccttttt
    16801 cttttaaaaa tgagaaaatt tcggtataat agtcaaacaa ggtcaaggtt taaagagaga
    16861 ggtgggtttg ttatgagatt taaaaataca tcggatcata ttgaggccta catcaaggcg
    16921 attttagatc aatctggtat cgtggagttg caacggagtc agttggcaga tacctttcag
    16981 gttgttccta gtcagattaa ctacgtgatc aagacacgct ttacggaaag tagaggctac
    17041 ttggttgaaa gtaagcgtgg tggcggaggc tacattcgta taggacggat tgagttttct
    17101 agtcatcatg aaatgctccg ggagctgctt tactcgattg gtgagcgagt cagtcaagaa
    17161 atttatgagg atattctcca gcttttggtt gagcaggaat tgatgaccaa gcaggagatg
    17221 aatttgctag aatcagtagc tttggatcgc gttttaggag aagaagctcc agttgttcga
    17281 gcaaacatgc tacgtcagat catacaagag gtagatagaa aagggaagta agatgaacta
    17341 ttcaaaagca ttgaatgaat gtatcgaaag tgcctacatg gttgctggac attttggagc
    17401 tcgttatcta gagtcttggc acttgttgat tgccatgtct aatcacagtt atagtgtagc
    17461 aggggcaact ttaaatgatt atccgtatga gatggaccgt ttagaagagg tggctttgga
    17521 actgactgaa acggactata gccaggatga aacttttacg gaattgccgt tctcccgtcg
    17581 tttgcagatt ctttttgacg aagcagagta tgtagcgtca gtggtccatg ctaaggtgct
    17641 aggtacagag cacgtcctct atgcgatttt gcatgatggc aatgccttgg cgactcgtat
    17701 cttggagagg gctggttttt cttatgaaga caagaaagat caggtcaaga ttgctgctct
    17761 tcgtcgaaat ttagaagaac gggcaggctg gactcgtgaa gatctcaagg ctttacgcca
    17821 acgccatcgt acagtagctg acaagcaaaa ttctatggcc aatatgatgg gcatgccgca
    17881 gactccgagt ggtggtctcg aggattatac gcatgatttg acagagcaag cgcgttctgg
    17941 caagttagaa ccagtcatcg gtcgggacaa ggaaatctca cgtatgattc aaatcttgag
    18001 ccgtaagact aagaacaacc ctgtcttggt tggggatgct ggtgtcggga aaacagctct
    18061 ggcgcttggt cttgctcagc gtattgccag tggtgatgtg cctgcggaaa tggctaagat
    18121 gcgcgtgtta gaacttgatt tgatgaatgt cgttgcaggg acacgcttcc gtggtgactt
    18181 tgaagaacgc atgaataata tcatcaagga tattgaagaa gatggccaag tcatcctctt
    18241 tatcgatgaa ctccacacca tcatgggttc tggtagcggg attgattcga ctctggatgc
    18301 ggccaatatc ttgaaaccag ccttggcgcg tggaactttg agaacggttg gtgccaccac
    18361 tcaggaagaa tatcaaaaac atatcgaaaa agatgcggca ctttctcgtc gtttcgctaa
    18421 agtgacgatt gaagaaccaa gtgtggcaga tagtatgact attttacaag gtttgaaggc
    18481 gacttatgag aaacatcacc gtgtacaaat cacagatgaa acggttgaaa cagcggttaa
    18541 gatggctcat cgttatttaa ccagtcgtca cttgccagac tctgctatcg acctcttgga
    18601 cgaagcagca gcaacagtgc aaaataaggc aaagtatgta aaagcagacg attcagattt
    18661 gagtccagct gacaaggccc tgatggatgg caagtggaaa caggcagccc agctaatcgc
    18721 aaaagaagag gaagtacctg tctacaaaga cttggtgaca gagtctgata ttttgaccac
    18781 cttgagtcgc ttgtcaggaa tcccagttca aaaactgact cagactgacg ctaagaaata
    18841 tctaaatctt gaagcagaac tccataaacg ggttatcggt caagatcaag ctgtttcaag
    18901 tattagtcgt gcgattcgcc gtaatcagtc aggaattcgc agtcacaagc gtccgatcgg
    18961 ttcctttatg ttcctaggac ccacaggtgt cggtaagacc gagttggcca aggctttggc
    19021 agaagttctc tttgatgacg aatcagccct tatccgcttt gatatgagtg agtatatgga
    19081 gaaattcgca gccagccgtc taaatggagc tcctccaggc tatgtgggtt acgaagaagg
    19141 tggggagttg acagagaagg ttcgcaataa accctattcc gttctcctct ttgatgaggt
    19201 agagaaggct cacccagata tctttaatgt tctcttgcag gttctggatg acggtgtctt
    19261 gacagatagc aagggacgca aggtcgattt ttcaaatacc attatcatta tgacatcgaa
    19321 cctaggtgcg actgcccttc gtgatgataa gactgttggt tttggggcta aggatattcg
    19381 ttttgaccag gaaaatatgg aaaaacgcat gtttgaagaa ctgaaaaaag cttatagacc
    19441 ggaattcatc aaccgtattg atgagaaggt ggtcttccat agcctatcta gtgaccatat
    19501 gcaggaagtg gtgaagatta tggtcaagcc tttagtggca agtttggctg aaaaaggcat
    19561 tgacttgaaa ttacaagctt cagctctgaa attgttagca aatcaaggat atgacccaga
    19621 gatgggagct cgcccacttc gcagaaccct gcaaacagaa gtggaggaca agttggcaga
    19681 acttcttctt aagggagatt tagtggcagg caacacactt aagattggtg tcaaagcagg
    19741 ccagttaaaa tttgatattg cataaaagaa taaaagtatc agcatctgac cataagtcac
    19801 agtggagtga aactcaatga aaatcaaaga gcaaactagg cagctagccg caggttgctc
    19861 aaaacactgg tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt
    19921 atgaaactaa aacctatagc ttctaaacga tccgtggttt tcaccattca acacaaaatt
    19981 catatgttta ttaccctccg tcgtatttgt cttagagcgt gtgtagtaga aaaagagcag
    20041 tcttatctga aatttttatt ctttcaaaag agacctgttt cttttttgca tgtcaaatcc
    20101 gttctagctg gtatttgaaa aatcaaacta atattcaatg aaaatcaaag aacaaactag
    20161 gaagctagcc gcaggttgct caaaacactg ttttgaggtt gtagatagag ctgacgtggt
    20221 ttgaagagat tttcgaagag tataagctgc aagatgaatg attttcttgt attgacgttg
    20281 ttgttgacaa aaagtagcgg ataaatgaaa tccattccat tatcatagat gataggctgg
    20341 taggaaattt tcaaatagca tacaggaaat agatgtatgg agttctggta gtagaaaggg
    20401 agagagatga acattttagt tgcagatgac gaggaaatga ttagagaagg aattgcagca
    20461 tttctgacag aagagggtta tcatgtcatt atggctaagg atggacaaga ggtcttggaa
    20521 aaatttcaag atctccctat ccatctcatg gtactggatt taatgatgcc taggaagagt
    20581 ggttttgaag tgttaaaaga aatcaatcaa aagcacgata ttcctgtcat cgtcttgagt
    20641 gctctgggag atgaaactac tcagtcacag gtatttgatc tctatgctga tgatcatgtg
    20701 acaaaacctt tttctttggt actgcttgtc aagcgtatta aggcgcttat cagacgttac
    20761 tacgtcatag aggatatttg gcgatatcag gatgtaacag tggattttac ctcttacaaa
    20821 gcacattata aaaatgaaga aattgatctc aaaccaaagg aattactggt actaaagtgt
    20881 ttgattcagc ataaaaatca agttttaagt agagagcaga tattggaaga aatttcaaaa
    20941 gatgtagctg atttaccttg tgatagggtc gttgatgtct atattcgtac tcttcgcaaa
    21001 aaattagctt tagattgtat cgtgactgtg aaaaatgttg ggtataagat tagcttatga
    21061 taaaaaatcc taaattatta accaagtctt ttttaagaag ttttgcaatt ctaggtggtg
    21121 ttggtctagt cattcatata gctatttatt tgacctttcc tttttattat attcaactgg
    21181 agggggaaaa gtttaatgag agcgcaagag tgtttacgga gtatttaaag actaagacat
    21241 ctgatgaaat tccaagctta ctccagtctt attcaaagtc cttgaccata tctgctcacc
    21301 ttaaaagaga tattgtagat aagcggctcc ctcttgtgca tgacttggat attaaagatg
    21361 gaaagctatc aaattatatc gtgatgttag atatgtctgt tagtacagca gatggtaaac
    21421 aggtaaccgt gcaatttgtt cacggggtgg atgtctacaa agaagcaaag aatattttgc
    21481 ttttgtatct cccatataca tttttggtta caattgcttt ttcctttgtt ttttcttatt
    21541 tttatactaa acgcttgctc aatcctcttt tttacatttc agaagtgact agtaaaatgc
    21601 aagatttgga tgacaatatt cgttttgatg aaagtaggaa agatgaagtt ggtgaagttg
    21661 gaaaacagat taatggtatg tatgagcact tgttgaaggt tattcatgag ttggaaagtc
    21721 gtaatgagca aattgtaaaa ttgcaaaatc aaaaggtttc ctttgtccgc ggagcatcac
    21781 atgagttgaa aaccccttta gccagtctta gaattatcct agagaatatg cagcataata
    21841 ttggagatta caaagatcat ccaaaatata ttgcaaagag tataaataag attgaccaga
    21901 tgagccactt attggaagaa gtactggagt cttctaaatt ccaagagtgg acagagtgtc
    21961 gtgagacctt gactgttaag ccagttttag tagatatttt atcacgttat caagaattag
    22021 ctcattcaat aggtgttaca attgaaaatc aattgacaga tgctaccagg gtcgtcatga
    22081 gtcttagggc attggataag gttttgacaa acctgattag taatgcaatt aaatattcag
    22141 ataaaaatgg gcgcgtaatc atatccgagc aagatggcta tctctctatc aaaaatacat
    22201 gtgcgcctct aagtgaccaa gaactagaac atttatttga tatattctat cattctcaaa
    22261 tcgtgacaga taaggatgaa agttccggtt tgggtcttta cattgtgaat aatattttag
    22321 aaagctatca aatggattat agttttctcc cttatgaaca cggtatggaa tttaagatta
    22381 gcttgtagac agattagttt tttattaaag ttcatatagg gttaacataa gtgtgttatt
    22441 ctttgtgtag a
//
LOCUS       DAVKZB010000022        22218 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKZB010000022 DAVKZB010000000
VERSION     DAVKZB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.022"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            108..977
                     /gene="rfbA"
                     /locus_tag="VXA80_000773"
     CDS             108..977
                     /gene="rfbA"
                     /locus_tag="VXA80_000773"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV0353306.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEHPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLGRGDLSVEVMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            978..1571
                     /locus_tag="VXA80_000774"
     CDS             978..1571
                     /locus_tag="VXA80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV0353307.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            1584..2633
                     /gene="rfbB"
                     /locus_tag="VXA80_000775"
     CDS             1584..2633
                     /gene="rfbB"
                     /locus_tag="VXA80_000775"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV0353308.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKVASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLREELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            2699..3550
                     /gene="rfbD"
                     /locus_tag="VXA80_000776"
     CDS             2699..3550
                     /gene="rfbD"
                     /locus_tag="VXA80_000776"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV0353309.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVARASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3626..4060
                     /locus_tag="VXA80_000777"
     CDS             3626..4060
                     /locus_tag="VXA80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0353310.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHIIHQKINLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            4136..4582
                     /locus_tag="VXA80_000778"
                     /pseudo
     CDS             4136..4582
                     /locus_tag="VXA80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4653..4811
                     /locus_tag="VXA80_000779"
     CDS             4653..4811
                     /locus_tag="VXA80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV0353311.1"
                     /translation="MSLLETAKRHQLNSEKYLFYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            5013..6995
                     /locus_tag="VXA80_000780"
     CDS             5013..6995
                     /locus_tag="VXA80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353312.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYIIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            7296..12599
                     /locus_tag="VXA80_000781"
     CDS             7296..12599
                     /locus_tag="VXA80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEV0353313.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPANLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGADVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFFVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTSLFLASVSLALSALFVVKTKKD"
     gene            complement(12766..14925)
                     /locus_tag="VXA80_000782"
     CDS             complement(12766..14925)
                     /locus_tag="VXA80_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV0353314.1"
                     /translation="MNKPTILRLIKYLNISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(14922..15518)
                     /gene="recU"
                     /locus_tag="VXA80_000783"
     CDS             complement(14922..15518)
                     /gene="recU"
                     /locus_tag="VXA80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV0353315.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            15584..16111
                     /locus_tag="VXA80_000784"
     CDS             15584..16111
                     /locus_tag="VXA80_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV0353316.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            16181..16510
                     /gene="gpsB"
                     /locus_tag="VXA80_000785"
     CDS             16181..16510
                     /gene="gpsB"
                     /locus_tag="VXA80_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV0353317.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            16531..16911
                     /gene="rnpB"
                     /locus_tag="VXA80_000786"
     ncRNA           16531..16911
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VXA80_000786"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            16996..18153
                     /locus_tag="VXA80_000787"
     CDS             16996..18153
                     /locus_tag="VXA80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV0353318.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KAWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            18166..19560
                     /gene="mapZ"
                     /locus_tag="VXA80_000788"
     CDS             18166..19560
                     /gene="mapZ"
                     /locus_tag="VXA80_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV0353319.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     PASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            19636..21060
                     /gene="gndA"
                     /locus_tag="VXA80_000789"
     CDS             19636..21060
                     /gene="gndA"
                     /locus_tag="VXA80_000789"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV0353320.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARFISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            21072..21761
                     /locus_tag="VXA80_000790"
     CDS             21072..21761
                     /locus_tag="VXA80_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0353321.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(21797..>22218)
                     /locus_tag="VXA80_000791"
     CDS             complement(21797..>22218)
                     /locus_tag="VXA80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0353322.1"
                     /translation="SDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHF
                     QNKDPEKFFGLIEDNLKQIHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIK
                     LIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT         6843 a         4037 c         5066 g         6272 t
ORIGIN      
        1 actttgatta attaaaaatt agcaaaattt attgacattt tgtttttata aattgcaata
       61 aaggtctaat tctgaatttc agtgaatata agaaagggat cctactaatg aaaggtatta
      121 ttcttgcagg tggttcgggg acacgattat atcctttgac tcgggctgca tcaaaacaac
      181 ttatgccggt ttatgataaa cccatgattt actatccact ttcaacattg atgttggctg
      241 ggattaggga tattttgata atctcaactc ctcaagattt gcctcgtttt aaagagcttc
      301 ttcaagacgg atctgagttt gggattaaac tttcttatgc agagcaacca agtccagatg
      361 gtttggcaca agcctttatc attggggaag agtttattgg tgatgatagt gttgctttga
      421 tcttggggga caatatctat cacggccctg gtttgagcaa aatgcttcaa aaagcagcca
      481 agaaagagaa aggtgcgact gtctttggct atcaagtgaa ggatccagag cgttttggtg
      541 tggttgagtt tgatacagac atgaatgcta tttctatcga agaaaagcca gagcaccctc
      601 gttcaaacta tgcagttaca ggactctatt tctatgataa tgatgtagta gagattgcca
      661 aaagtataaa accaagccct cgtggtgaac tggaaattac agatgtaaac aaggcttacc
      721 taggtcgtgg agatttatcc gttgaggtta tgggacgagg ctttgcttgg ctggatactg
      781 gcactcatga aagtttatta gaggcttcgc agtacatcga aacagtccaa cggatgcaaa
      841 atgttcaggt ggcaaacttg gaagaaattt cttaccgtat gggctatatc agtcgagaag
      901 atgtattggc cttagcccaa ccacttaaga aaaatgaata cggacagtat ctgctccgtt
      961 tgattggaga agcatagatg acagataatt ttttcggtaa gacgcttgcg gcacgcaagg
     1021 ttgaagctat tccaggcatg ttggagtttg atatccccgt tcatggagat aatcgtggct
     1081 ggtttaaaga aaatttccaa aaggaaaaaa tgcttccact tggatttcca gagtctttct
     1141 ttgcagaagg aaaattgcaa aacaatgtat ccttctcacg taaaaatgtc cttcgaggcc
     1201 tccacgcaga gccttgggat aagtacatct ctgtagcaga tggagggaaa gttctgggtt
     1261 cttgggttga tctacgcgag ggtgaaacct ttgggaatac ctatcagaca gtgattgatg
     1321 cgagtaaggg aatctttgtt cctcgaggcg tagctaatgg cttccaagtt ttatcagata
     1381 cagtgtcata tagctatctg gtcaatgatt actgggctct tgaactcaaa cccaagtatg
     1441 cctttgtgaa ctacgctgat ccaagccttg gtattgaatg ggaaaatatt gcagaagcag
     1501 aggtttcaga agcagataaa catcatcccc tacttaagga tgtaaaacct ttgaaaaaag
     1561 aagatttgta aaaaggaaag aatatgactg aatacaaaaa tattatcgtg acaggtggag
     1621 ctggctttat cggttctaac tttgtccatt atgtttacga gaactttcca gatgttcatg
     1681 tgacagtcct agataagttg acttatgctg gaaaccgcgc gaatattgag gaaattttag
     1741 gtaatcgtgt tgagttagtt gttggtgaca ttgctgatgc ggagttggta gacaagttgg
     1801 ctgctcaagc agatgctatc gttcattatg cagcggaaag ccacaatgat aattcgctca
     1861 atgatccatc gccatttatt catactaact tcatcggaac ctatactctt ttagaagctg
     1921 ctcgtaagta tgatattcgc ttccaccatg tatcgacaga tgaagtttat ggggatctcc
     1981 ctttacgcga agatttgcca ggtcatggtg aaggaccagg tgagaaattt actgctgaaa
     2041 caaaatacaa cccaagctct ccgtactcat caaccaaggt agcctcagat ttgattgtca
     2101 aagcctgggt gcgttctttt ggagtcaagg caacgatttc caactgttca aataactacg
     2161 gtccttatca acatatcgaa aaattcatcc cacgtcagat tactaacatc ctaagtggta
     2221 tcaagccaaa actttacggt gaaggtaaga acgttcgtga ttggattcat accaatgacc
     2281 attcttcagg agtttggaca atcttgacaa aagggcaaat cggtgaaacc tacttgattg
     2341 gggctgatgg tgagaagaac aataaggaag ttttggaact tatccttaag gaaatgggac
     2401 aagctgcgga tgcctatgat catgtgactg accgtgcagg acatgacctt cgctatgcga
     2461 ttgatgctag caagctccgt gaggagttgg ggtggaaacc tgaatttacc aactttgaag
     2521 ctgggctcaa ggcaacaatc aagtggtata cagataacca agaatggtgg aaagcagaaa
     2581 aagaagctgt tgaagccaat tatgctaaaa ctcaggagat tattacagta taaaaagcag
     2641 gaaatagctg ctttttattg ctatattggg aagagttaca tattagaaag gtctagagat
     2701 gattttaatt acaggggcaa atggccaatt aggaacggaa cttcgctatt tattggatga
     2761 acgtaatgaa gaatacgtgg cagtagatgt ggctgagatg gacattacca atgaagaaat
     2821 ggttgaaaaa gtttttgaag aggtgaaacc gactttagtc taccactgtg cagcctacac
     2881 cgctgttgat gcagcagagg atgaaggaaa agagttggac ttcgccatca atgtgacggg
     2941 gacaaaaaat gtcgcaagag catctgaaaa gcatggtgca actctagttt atatttctac
     3001 ggactatgtc tttgacggta agaaaccagt tggacaagag tgggaagttg atgaccgacc
     3061 agatccacag acagaatatg gacgcactaa gcgtatgggg gaagagttag ttgagaagca
     3121 tgtgtctaat ttctatatta tccgtactgc ctgggtattt ggaaattatg gcaaaaactt
     3181 cgtttttacc atgcaaaatc ttgcgaaaac tcataagact ttaacagttg taaatgacca
     3241 gtacggtcgt ccgacttgga ctcgtacctt ggctgagttc atgacctacc tagctgaaaa
     3301 tcgtaaggaa tttggttatt atcatttgtc aaatgatgcg acagaagaca caacatggta
     3361 tgattttgca gttgaaattt tgaaagatac agatgtcgaa gtcaagccag tagattccag
     3421 tcaatttcca gccaaagcta aacgtccgct aaactcaacg atgagcctgg ccaaagccaa
     3481 agctactgga tttgttattc caacttggca agatgcattg caagaatttt acaaacaaga
     3541 agtgagataa gtagtagaat gattttctag tctaataaaa gaggcagata atgaactcca
     3601 aaggagctta agatgtacga ttatcttgtt gttggtgctg gtctctttgg cgcatagctt
     3661 tggctcagtt tctattatcg ctcacatcat ccatcagaag attaatctga aggtacccaa
     3721 ttatcgccaa gaagaagatt gggctaggat gggtttacca atcacacgta aggaaatctc
     3781 taattggcat atcaaggcaa gtcaatacta tttagagtcc ctttataacc ttttacgaga
     3841 aaagttgtta gaacaacctc ttcttcatgc ggatgaaacc tcttatcggg ttctagagag
     3901 tgatagccat ctgacctact attggacctt tttgtctggg aaagctgaga atcaagcaat
     3961 cacgctgtac catcatgatc agcgtcggag tggtttagta gtacaagaat tcctaggaga
     4021 ttattctggc tatgtgcatt gtgatatgtt gcggcagtaa cttaggactt tagtcctcta
     4081 gttctgccta tgcgatagca gtccaaggtt taggagcaag gcgacgctaa gcttggtaaa
     4141 ctgcgaaccg ctagaagctt atcgtcaact ggaagaagct gaacttgttg gatgttgggc
     4201 acatgtgaga aggaagtttt ttgaagcgac ccccaagcaa gcagataaat catccttaga
     4261 agctaaaggt ttagcttatt gtgatcagtt attttccctg gaaagagact gggaggcttt
     4321 gccagctgat gaacgactac agaaacgtca agaagagctc caacccctaa tggaagactt
     4381 ctttgcttag tgccggcgtc agtcagtttt agcaggttca aaactaggaa gggcaattga
     4441 atacagcctc aagtatgaag aaacctttaa gactattttg aaagacggac atctggtcct
     4501 ttccaataat ctagctgaac gcgccattaa atcattggtt atgggacgga gtaaaagagt
     4561 tcagtggact cttttagcct aagctaaatt ttaaaaagcg agggtggtta ttttctcaaa
     4621 gttttgaagg agctaaagca acagctatta ttatgagttt gttggaaaca gctaaacgtc
     4681 atcaattaaa tagcgagaaa tatctattct atcttctaga atgtcttcca aacgaggaaa
     4741 ctctcgtaaa caaagaggtt ttagaggctt atttaccatg gactaaagtt gtacaagaaa
     4801 agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctctagt ctggagattt
     4861 ttcaatatac ttcgttattg ggcgcttaca atgatattca tattttttgc aaagatgttg
     4921 tttgaaaaat aattttcaaa aattctgaaa attctgttga caactttctg aaaagagtct
     4981 ataatggaga gaaagtttta aaggagaaaa tgatgaaaag ttcaaaacta tttgcccttg
     5041 cgggcgtgac attattggcg gcgactactt tagctgcatg ctctggatca ggttcaagcg
     5101 ctaaaggtga gaagacattc tcatacattt atgagacaga ccctgataac ctcaactatt
     5161 tgacaactgc taaggctgcg acagcaaata ttaccagtaa cgtggttgat ggtttgctag
     5221 aaaatgatcg ctacgggaac tttgtgccgt ctatggctga ggattggtct gtatccaagg
     5281 atggattgac ttacacttat attatccgta aggatgcaaa atggtatact tctgaagggg
     5341 aagaatacgc ggcagtcaaa gctcaagact ttgtaacagg actaaaatat gctgctgata
     5401 aaaaatcaga tgctctttac cttgttcaag aatcaatcaa agggttggat gcctatgtaa
     5461 aaggggaaat caaagatttc tcacaagtag gaattaaggt tctggatgaa cagacagttc
     5521 agtacacttt gaacaaacca gaaagcttct ggaattctaa gacaaccatg ggtgtgcttg
     5581 cgccagttaa tgaagagttt ttgaattcaa aaggagatga ttttgccaaa gctacggatc
     5641 caagtagtct cttgtataac ggtccttatt tgttgaaatc cattgtgacc aaatcctctg
     5701 ttgaatttgc gaaaaatccg aactactggg ataaggacaa tgtgcatatt gacaaagtta
     5761 aattgtcatt ctgggatggt caagatacca gcaaacctgc agaaaacttt aaagatggta
     5821 gccttacagc agctcgtctc tatccaacaa gtgcaagttt cgcagagctt gagaagagta
     5881 tgaaggacaa tattgtctat actcaacaag actctattac gtatctagtc ggtacaaata
     5941 ttgaccgtca gtcctataaa tacacatcta agaccagcga tgaacaaaag gcatcgacta
     6001 aaaaggctct cttaaacaag gattttcgtc aggctattgc ctttggtttt gatcgtacag
     6061 cctatgcctc tcagttgaat ggacaaactg gagcaagcaa aatcttacgt aatatctttg
     6121 ttccaccaac atttgttcaa gcagatggta aaaactttgg cgatatggtc aaagagaaat
     6181 tggttactta tggggatgaa tggaaggatg ttaatcttgc agattctcag gatggtcttt
     6241 acaatccaga aaaagccaag gctgaatttg ctaaagctaa atcagcctta caagcagaag
     6301 gtgtgacttt cccaattcat ttggatatgc cagttgacca gacagcaact acaaaagttc
     6361 agcgcgtcca atctatgaaa caatccttgg aagcaacttt aggagctgat aatgtcatta
     6421 ttgatatcca acaactacaa aaagacgaag taaacaatat tacatatttt gctgaaaatg
     6481 ctgctggcga agactgggat ttatcagata atgtcggttg gggtccagac tttgccgatc
     6541 catcaaccta ccttgatatc atcaaaccat ctgtaggaga aagtactaaa acatatttag
     6601 ggtttgactc aggggaagat aatgtagctg ctaaaaaagt aggtctatat gactacgaaa
     6661 aattggttac tgaggctggt gatgaggcta cagatgttgc taaacgctat gataaatacg
     6721 ctgcagccca agcttggttg acagatagtg ctttgattat tccaactaca tctcgtacag
     6781 ggcgtccaat cttgtctaag atggtaccat ttacaatacc atttgcattg tcaggaaata
     6841 aaggtacaag tgaaccaatc ttatataaat acttggaact tcaagacaag gcagtcactg
     6901 tagatgaata ccaaaaagct caggaaaaat ggatgaaaga aaaagaagag tctaataaaa
     6961 aggctcaaga agatctcgca aaacatgtga aataactgtt gcaaaatata agaaaggatt
     7021 tagtatttct cttgaatgct gaatcctttt ttacatttgt aaagaaagat tctaaatgta
     7081 ctgaccccca aaagttggag cctctttttg tcagaataga gaaaattttt gttaatttta
     7141 cttgtttcct attgctttct cagctattat ttgttatatt aaaagtataa ttatttttta
     7201 tttatcaggg ttaagcattg cactttcaga ggaaggagta ttttttaaaa agaaaatgta
     7261 aacgcttgct aaaaaatgaa aggatttaga agtttatgaa taaaggatta tttgaaaaac
     7321 gttgtaaata tagtattcgg aaattttcat taggtgttgc ttctgttatg attggagctg
     7381 cattctttgg gacaagtccg gttcttgcag atagcgtgca gtctggttcc acggcgaact
     7441 taccagctaa tttagctact gctcttgcaa cagcaaaaga gaatgatgga cgtgattttg
     7501 aagcgcctaa ggtgggagaa gaccaaggtt ctccagaagt tacagatgga cctaagacag
     7561 aagaagaact attagcactt gaaaaagaaa aaccagctga agaaaaacca aaagaggata
     7621 aacctgcagc tgctaaacct gaaacaccta agacggtaac ccctgaatgg caaacggtag
     7681 agaaaaaaga acaaaaggga acagtcacta tccgagaaga aaaaggtgtc cgctacaacc
     7741 aactatcctc aactgctcaa aatgataacg caggtaaacc agccctgttt gaaaagaagg
     7801 gcttgaccgt tgatgccaat ggaaatgcaa ctgttgattt aaccttcaaa gaagattctg
     7861 aaaagggcaa atcacgcttt ggtgtcttct tgaaatttaa agataccaat aataatgttt
     7921 ttgtcggtta tgacaaggat ggctggttct gggagtataa atctccaaca actagcactt
     7981 ggtatagagg tagtcgtgtt gctgctcctg aaacaggatc aacaaaccgt ctctctatca
     8041 ctctcaagtc agacggtcag ctaaatgcca gcaataacga tgtcaatctc tttgacacag
     8101 tgactctacc agctgcggtc aatgaccatc ttaaaaatga gaagaagatc cttctcaagg
     8161 cgggctctta tggcaatgac cgtacggttg tcagcgttaa aacagacaat caagaaggcg
     8221 taaaagcgga tgatactcct gcccagaaag aaacaggtcc ggttgttgac gatagcaagg
     8281 tgacttatga tacgatccag tctaaggtac ttaaggcagt aattgaccaa gccttccctc
     8341 gtgtcaagga atacagcttg aatggacata ctttgccagg acaggtgcaa cagttcaacc
     8401 aagtctttat caataaccac cgaatcaccc ctgaagtcac ttataagaaa atcaatgaga
     8461 caacagcaga gtacttgatg aagattcgcg atgatgctca cttaatcaat gcggaaatga
     8521 cagtacgctt gcaagttgtg gacaatcaat tgcactttga tgtgaccaag attgtcaacc
     8581 acaatcaagt cactccaggt caaaagattg atgacgaaag aaaactactt tcttctatta
     8641 gtttcctcgg caatgcttta gtctctgttt ctagtgatca aactggtgct aagtttgatg
     8701 gggcaaccat gtcaaacaat acgcatgtca gcggagatga tcatatcgat gtaaccaatc
     8761 caatgaagga tttggctaag ggttacatgt atggatttgt ttctacagat aagcttgctg
     8821 ctggtgtttg gagtaactct caaaacagct atggtggtgg ttcgaatgac tggactcgtt
     8881 tgacagctta taaagaaaca gtcggaaatg ccaactatgt aggaatccac agctctgaat
     8941 ggcaatggga aaaagcttat aagggcattg ttttcccaga atacacgaag gaacttccaa
     9001 gtgctaaggt tgttatcact gaagatgcca atgcagacaa gaaagttgat tggcaagatg
     9061 gtgccattgc ttatcgtagc attatgaaca accctcaagg ttgggaaaaa gttaaggata
     9121 tcacagctta ccgtatcgcg atgaactttg gttctcaagc acaaaaccca ttccttatga
     9181 ccttggatgg tatcaagaaa atcaatctcc acacagatgg tcttgggcaa ggtgttctcc
     9241 ttaaaggata tggtagcgaa ggccatgact ctggtcactt gaactatgct gatattggta
     9301 agcgtatcgg tggtgtcgaa gacttcaaga ccctaattga gaaggctaag aaatatggag
     9361 ctcatctagg tatccacgtt aacgcttcag aaacttatcc tgagtctaaa tacttcaatg
     9421 aaaaaattct ccgtaagaat ccagatggaa gctatagcta tggttggaac tggctagatc
     9481 aaggtatcaa cattgatgct gcctatgacc tagctcatgg tcgtttggca cgttgggaag
     9541 atttgaagaa aaaacttggt gacggtctcg actttatcta tgtggacgtt tggggtaatg
     9601 gtcaatcagg tgataacggt gcctgggcta cccacgttct tgctaaagaa attaacaaac
     9661 aaggctggcg ctttgcgatc gagtggggcc atggtggtga gtacgactct accttccatc
     9721 actgggcagc tgacttgacc tacggtggct acaccaataa aggtatcaac agtgccatca
     9781 cccgctttat ccgtaaccac caaaaagatg cttgggttgg ggactacaga agttatggtg
     9841 gtgcagccaa ctatccactg ctaggtggct acagcatgaa agactttgaa ggctggcaag
     9901 gaagaagtga ctacaatggc tatgtgacta acctctttgc tcatgacgtc atgactaagt
     9961 acttccaaca cttcactgta agtaaatggg aaaatggtac accggtgact atgaccgata
    10021 acggtagcac ctataaatgg actccagaaa tgcgagtgga gttggtagat gctgacaata
    10081 ataaagtagt tgtaactcgt aagtcaaatg atgtcaatag cccacaatat cgcgaacgta
    10141 cagtaacgct caacggacgt gtcatccaag atggttcagc ttacttgact ccttggaact
    10201 gggatgcaaa tggtaagaaa ctttctactg ataaggaaaa gatgtactac ttcaatacgc
    10261 aggccggtgc aacaacttgg acccttccaa gcgattgggc aaagagcaag gtttaccttt
    10321 acaagctaac tgaccaaggt aagacagaag agcaagaact aactgtaaaa gatggtaaaa
    10381 ttaccctaga tcttctagca aatcaaccat acgttctcta tcgttcgaaa caaactaatc
    10441 ctgaaatgtc atggagtgaa ggcatgcaca tctatgacca aggatttaat agcggtacct
    10501 tgaaacattg gaccatttca ggcgatgctt ctaaggcaga aattgtcaag tctcaagggg
    10561 caaacgatat gcttcgtatt caaggaaaca aagaaaaagt tagtctcact cagaaattaa
    10621 ctggtttgaa accaaatacc aagtatgccg tttatgttgg tgtagataac cgtagtaatg
    10681 ccaaggcaag tatcactgtg aatactggtg aaaaagaagt gactacttat accaataagt
    10741 ctctcgcgct caactatgtt aaggcctacg cccacaatac acgtcgtgac aatgctacag
    10801 ttgacgatac aagttacttc caaaacatgt acgccttctt tacaactgga gcggacgtct
    10861 caaatgttac tctgacattg agtcgtgaag ctggtgatca agcaacttac tttgatgaaa
    10921 ttcgtacctt tgaaaacaat tcaagcatgt acggagacaa gcatgataca ggtaaaggca
    10981 ccttcaagca agactttgaa aatgttgctc agggtatctt cccatttgta gtgggtggtg
    11041 tcgaaggtgt tgaagataac cgcactcact tgtctgaaaa acacaatcca tatacacaac
    11101 gtggttggaa tggtaagaaa gtcgatgatg ttatcgaagg aaattggtca ctcaagacaa
    11161 atggactagt gagccgtcgt aacttggttt accaaaccat cccacaaaac ttccgttttg
    11221 aagcaggtaa gacctaccgt gtaacctttg aatacgaagc aggatcagac aatacctatg
    11281 cttttgtagt cggtaaggga gaattccagt caggtcgtcg tggtactcaa gcaagcaact
    11341 tggaaatgca tgaattgcca aatacttgga cagattctaa gaaagccaag aaggcaacct
    11401 tctttgtgac aggtgcagaa acaggcgata cttgggtagg tatctactca actggaaatg
    11461 caagtaatac tcgtggtgat tctggtggaa atgccaactt ccgtggttat aacgacttca
    11521 tgatggataa tcttcaaatc gaagaaatta ccctaacagg taagatgttg acagaaaatg
    11581 ctctgaagaa ctacttgcca acggttgcca tgactaacta caccaaagag tctatggatg
    11641 ctttgaaaga ggcggtcttt aacctcagtc aggccgatga tgatatcagt gtggaagaag
    11701 cgcgtgcaga gattgccaag attgaagctt tgaagaatgc tttggttcag aagaaaacgg
    11761 ctttggtagc agatgacttt gcaagtctta cagctcctgc tcaggctcaa gaaggtcttg
    11821 caaatgcctt tgatggcaat gtgtctagtc tatggcatac atcttggaat ggtggagatg
    11881 taggcaagcc tgcaaccatg gtcttgaaag aaccaactga aatcactgga cttcgttatg
    11941 ttccacgtgg atcaggttca aatggtaact tgcgtgatgt gaaacttgtt gtgacagatg
    12001 agtctggcaa ggagcatacc tttactgcaa ctgattggcc agataacaat aagccaaaag
    12061 acattgattt tggtaagaca attaaggcta agaaaattgt ccttacaggt actaagactt
    12121 acggagatgg tggcgataaa taccaatctg cagcggaact catctttact cgtccacagg
    12181 tagcagaaac acctcttgac ttgtcaggct atgaagcagc tttggctaaa gctcagaaat
    12241 taacagacaa agacaatcaa gaggaagtag ctagtgttca ggcaagcatg aaatatgcga
    12301 cggataacca tctcttgacg gaaagaatgg tggaatactt tgcagattat ctcaaccaat
    12361 taaaagattc tgctacgaaa ccagatgctc caactgtaga gaaacctgag tttaaactta
    12421 gctctttagt ttccgagcaa ggtaagacgc cagattataa gcaagaaata gctagaccag
    12481 aaacacctga acaaatcttg ccagcaacag gtgagagtca atctgacaca tccctcttcc
    12541 tagcaagtgt tagtctagcc ctatctgctc tctttgtagt aaaaacgaag aaagactagt
    12601 atttagtaaa acctcttaac aagattacgg aagcagtctc tatcttttcc aatgaggttt
    12661 atagtacaga aaaagcctga gaagatgtct tctcaggctt ttgttaagca cataaataca
    12721 atagtgctat gacaaaatca cccagaaaaa tctgggtgat aaatgttatg gttgtgctgg
    12781 ttgaggattc tgattttgtt gatcaggggt tgtatttgat tgttgcgtat tattgttagg
    12841 attggtagtc gtactattat ttgtgcttgg agtggttgag ctagactgtg aagttgaact
    12901 atctgatgat gagcttgaac tttcagttga tgggggttgt tgtggagcag gtgagttcca
    12961 cgtagaacga gcaccatttt taaatacgaa ttctccattt ctgtagagcc cctctggtat
    13021 attccaatct tctggattgc ttccttcaga caggtaggtc atcatagagc ggtaaacttt
    13081 ggcagcgact gtaaggccat tgcctacaag tggtgtcaga cggttagaat agcctgtcca
    13141 tacagccatt gaatatttac gcgtatagcc agcaaatagt tcgtcaggtg ctacaaattg
    13201 agaggtcttg atgtggtttt caatttcctc gtctgtatag ttagaggttc ctgttttacc
    13261 agcctgaggg agccaagcaa gataggcatt tcgtccagtt ccataactca agactgtttt
    13321 catcatgtcg gtcatcatat aggctgtcgt ttccttcatg gcacgagttc cgacattaga
    13381 gaactctttt tcactcccat cactaaagac gactttatgg atatacattg gtttatagta
    13441 agttccacca tttgcaaagg cagcgtaagc agcagccatc ttttcactac ttgctccata
    13501 ttttttgtct gattcggttg tgttacttga aatggcattt gagtagtgaa tacttgggta
    13561 gtcgattccg agaccattta ggaaagtctt ggcgcggttg agtccgacct tgtttagagt
    13621 ttccacggct gggacgtttc gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa
    13681 gtagccccta tcccagttat aaacaggggt atttgtccca gggtagttat agggttcatc
    13741 gtgaacgata gtagcagttg aatcgtagac accgtactcc aaggcaggag catagtctgt
    13801 gatcggtttc atagttgatc cccagtcgcg gtttgtttct actgcttggt taattccgaa
    13861 ggaaacatta cttgactgat ggcgtgctcc tagctgggca atgactttac cgttagaaac
    13921 atcaacaatg gtagaagcga cttgcaattc atcgtctgga taggcaacgt attcgtctgt
    13981 attgtaaata tcccacagat gtttttgagc ttcttggtct acatttgtgt agacatccat
    14041 cccagttgtg agcaggttat agcctgtttc ttcttcaact tgattgatga cttccttgag
    14101 gtaattatcc atgtaagcag ggtaattact tgctgatttg agactttgta gtccatcagt
    14161 aattggtgta ttgactgctt tctcatactg ttcagcagag atgtagcctt gatttttcat
    14221 ttcagataag accaagtttc ggcggtcttg ggctgcttct ggatgtgaat aggggtcata
    14281 ttggtttggt gcctgaggca ttccagccag caaggctaac tgaggtaaac ttaaattatt
    14341 gaggtcttta ccatagtagt tttgagctgc tgtctgcatt ccatagttcc cattagacat
    14401 gtagacctta tttatatagt aggtcaagat ttcttgcttg gttgcttttt gttctaactg
    14461 aatcgctaac caagcttcct gagccttacg agaaatagtc tggtcggaag tcgaagttga
    14521 aaagtaagtc aacttaatca actgttgggt gagagttgat ccaccttgga gggaattgct
    14581 ttgcagattg cgcaagaaag ctcccaggat acggatggta tcaatccccc tgtggtcgaa
    14641 gaagcgatgg tcttcgatag aaacgattgc cttaaccaaa tctgtgggaa tatcattagc
    14701 ttgggcattg acgcggcgtt cagaacccaa gtcagcaatg agttgatttt tattgtcgta
    14761 gattttacta gaagttgttg caactagttt actctcggat aggctaggag ccttgctaac
    14821 gtagtagaaa aaaactcctc cgcctaagac aatggctgcg ataaccaagc ttaagaagct
    14881 aatgttcaga tacttgatta ggcgcagaat cgttggtttg ttcatcttgt tttaccacct
    14941 aataaatgtt ctttgataac attgagataa ggaatttgag ggaaggcacc agccttgatt
    15001 tcatatccat attctcgaat atattcaagt ggcattgatt tttgtccctt atcttgatga
    15061 tagaagcgaa tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga agaaaagtga
    15121 agaaggacaa agcagattcc ttgttgggca aggacttgtt ccatatgctg aatctgatgt
    15181 ggatgaaaat ttttcatcgg aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg
    15241 atgtaatatc cattataaac gccagaatag tccgtcgttg aagcttgtcg aaaataggct
    15301 tcaacaatct tggcacgact tcgttgtgga tagtccactt gtacgatttg aataggagtt
    15361 ggtttcttat gtataacagc caagccctga gacaaatagt agtcgttggt agcattgatc
    15421 atcttttcaa aagacattcc tcgatttgcg aaatttttgg gttgagaaag agatgtttgt
    15481 cttttttgtg atgaaacttt atgtggatag ttgaccataa ttctccttat tggtacaata
    15541 acatcacttt attataccat aatttgcaca gaaagggtta aaaatggcta cagctttggt
    15601 tttaggctat tctgcttttg atttgggttt attttctgat aaggatcctc gtcttaaatt
    15661 gattaaaaaa gcgatacgta aagatttaga ggctatggca gcagatgggg tgtcttggct
    15721 tgtgtttaca gggactttgg gctttgaata ttgggtttta gaggttgctc aggagatgaa
    15781 aaccgaatac ggtttccagt tggccaccat ttttgctttt gaaacccatg gggaaaattg
    15841 gaatgaaggc aatcagatga aactgagtcg ctttaagcag gtagactttg tcaaatatgc
    15901 ctatcctcgc tatgaacaca aggggcagtt aagagattac cagcagtttt tgctggaaaa
    15961 cacgactagt tcctatcttt tttatgatga agaaaatgaa acaaaactag cttattttta
    16021 ccaaaagatg aaaaatcaag aggactattt tataaagaga ttaacatttg atgaattaaa
    16081 tgaacttgct gaaaattttt ccgaaaattg aagctttgac cttgattttt gtttgccttt
    16141 ttttatataa taatactagc aagcgagaat ggagagagac atggcaagta ttattttttc
    16201 agcgaaagat atttttgaac aagagtttgg acgtgaagtc cgtggctata ataaagtaga
    16261 agttgacgag tttttagacg atgtcatcaa ggactatgaa acctatgctg ccttggtcaa
    16321 gtcacttcgt caggaaattg cggatttgaa ggaagaatta actcgtaaac cgaaaccttc
    16381 accagttcaa gcagaacccc ttgaagcggc aattacaagt tctatgacga attttgatat
    16441 tttgaaacgc ctgaatagat tggaaaaaga agtttttggt aaacaaattt tagataactc
    16501 agatttttaa gtagttattt gagatgtgca atttttggat aatcgcgtga ggagaattgc
    16561 ttctcatgag gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc
    16621 gaaaccataa gcctagggac gagaaatcgt tacggcagtt gaaatggcta agtccttgga
    16681 taggccagag taggcttgaa agtgccacag tgacggagtc tttctggaaa cagagagagt
    16741 ggaacgcggt aaacccctca agctagcaac ccaaattttg gtcggggcat ggagtacgcg
    16801 gaaacgaacg tagtattctg actgctatca gctagagctg ttagtggtag acagatgatt
    16861 atcgaaggaa gtggtcctag tcacttctgg aacaaaacat ggcttataga aaattgcata
    16921 taggttgggg ctgagaaatt ttctcaacct cattttttaa agtggacata tagaaaggtc
    16981 ttgcaagact gtaacatgaa aaaagaattt aatttaattg caactgtggc agcagggctt
    17041 gaggctgtcg ttggtcgtga agtgcgagag ttgggctacg attgtcaggt tgaaaatgga
    17101 cgtgttcgtt ttcaaggaga cgtgagagct attatcgaaa ccaacctttg gcttcgggca
    17161 gcagatcgta tcaaaattat cgtaggaacg ttcccagcta agacttttga agagctattt
    17221 cagggagttt tcgctttaga ttgggaaaat tatttaccac ttggagctcg gttcccgatt
    17281 tcaaaagcta aatgtgttaa gtccaaactt cacaatgagc ccagtgttca ggctatttct
    17341 aagaaagctg ttgtcaagaa attgcagaaa cactatgctc gcccagaagg ggttcctctg
    17401 atggagaatg gcccagagtt taagattgag gtctctattc tcaaagatgt ggcaactgtc
    17461 atgattgata cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc
    17521 gctcctatca aggaaaatat ggcagcagcc attttacaac tttctaactg gtatccagac
    17581 aagcctttga ttgatccgac ctgtggttcg gggactttct gtattgaggc agttatgatt
    17641 gctagaaaga tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc
    17701 agcgatcgtt tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga ccgtgagctt
    17761 gagctggata tcatgggctg tgatattgat gctcgcatgg tggaaattgc taaggccaat
    17821 gctcaggtag ctggtgttgc aggagacatt acttttaagc agatgcgcgt gcaggattta
    17881 cgttccgata aaatcaatgg agtaatcatt tccaatccgc cttatggtga acgtttgtca
    17941 gatgatgcag gggtgaccaa gctctatgct gagatggggc aagtatttgc accgctgaaa
    18001 gcttggagca aatttatcct gactagtgat gaagcttttg aaagcaagta tggtagccaa
    18061 gcagataaga agcgtaagtt atacaacgga accttgaaag tggatctata tcaatatttt
    18121 ggtcagcgtg tcaaacggca agaggtaaaa tagaaaggga tactcatgag taaaaaaaga
    18181 cgaaatcgtc ataaaaaaga aggtcaagaa ccgcaatttg attttgatga agcaaaagag
    18241 ctaacagttg gtcaagctat tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag
    18301 gattccattt tggacaagta tgttaagcaa cacagagatg aaattgaggc ggataagttt
    18361 gcgactcgtc aatacaaaaa agaggagttc gttgaaactc agagtctgga tgatttaatt
    18421 caagagatgc gtgaggctgt agagaagtca gaagcttctt cggaggaagt tccatcttct
    18481 gaagacatct tactaccctt gcctctggac gatgaggagc aaggcttgga tcctctattg
    18541 ctagatgatg aaaatccaac agaaatgact gaagaagtgg aagaggagca aaacctttct
    18601 cgtctggatc aagaggactc agaaaagaaa agtaaaaaag gctttatttt gaccgttttg
    18661 gcgcttgtat cagtaattat ttgtgtcagt gcttattatg tctaccgtca agtggctcgt
    18721 tcgaccaagg aaattgaaac ttctcaatca actacagcca atcaatcgga tgtggatgat
    18781 tttaatacac tttatgacgc cttttacaca gatagcaata aaacggcttt gaaaaatagc
    18841 cagtttgata aactgagtca actcaaaacc ttgcttgata aactggaagg tagtcgtgaa
    18901 catacgcttg ccaaatctaa atatgatagt ctagcaacgc aaatcaaggc tattcaagat
    18961 gtcaatgcac aatttgaaaa atcagctatt gtggatggtg tgttggatac caatgccaaa
    19021 gccaaatcgg atgctaaatt tacagatatt aaaactggaa atacggagct tgataaagtg
    19081 ctagataagg ctatcagtct tggtaagagc cagcaaacaa gtacttctag ctcaagttca
    19141 agtcaaacta gcagctcaag ttcaagtcca gcaagctcaa atacgacaag tgagccaaaa
    19201 ccaagtagtt caaatgagac tagaagtagt cgcagtgaag tcaatatggg tctctcgagt
    19261 gcaggggttg ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc tgctattgat
    19321 gatagtaata actctgcctg ggattttgcg gatggtgtct tggaacaaat tctagcaact
    19381 tcacgttcac gtggctatat cactggagac caatatatcc ttgaacgtgt caatatcgtt
    19441 aacggcaatg gttattacaa cctctataag ccagatggaa cctatctctt tacccttaac
    19501 tgtaagacag gatattttgt cggaaatggc gctggtcatg cggatgactt ggattactaa
    19561 gcagtcgtta caaaattctt tcttttcaaa agtaaaaatg ataaaataaa acaaatgaaa
    19621 caagaggagt gtcaaatgac aaaagctaac tttggtgttg taggtatggc cgtaatgggt
    19681 cgtaaccttg cccttaatat tgaatctcgt ggttacacag ttgctatcta caaccgtagt
    19741 aaagaaaaaa cggaagatgt gattgcttgc catcctgaaa agaattttgt accaagctat
    19801 gacgttgaaa gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct gatggttcaa
    19861 gctggacctg gtacagatgc tactatccaa gcccttcttc cgcaccttga caagggtgat
    19921 atcttgattg atggaggaaa cactttctac aaagatacca tccgtcgtaa tgaagaattg
    19981 gcaaactcag gtatcaactt tatcggtact ggagtttctg gtggtgaaaa aggtgccctt
    20041 gaaggtcctt ctatcatgcc tggtggacaa aaagaggcct acgaattggt tgctgatgtt
    20101 cttgaagaaa tctcagctaa agcaccagaa gatggcaaac catgtgtgac ttatatcggt
    20161 cctgatggag ctggtcacta tgtgaaaatg gttcacaatg gtattgagta cggtgatatg
    20221 caattgatcg cagaaagcta tgacttgatg caacacttgc taggcctttc tgcagaggat
    20281 atggctgaaa tctttactga gtggaacaag ggtgaattag acagctactt gattgaaatc
    20341 acagctgata tcttgagtcg taaagacgat gaaggccaag atggaccaat cgtagactac
    20401 atccttgatg ctgcaggtaa caagggaact ggtaaatgga cgagccaatc atctcttgac
    20461 cttggtgtac cattgtcact gattactgag tcagtgtttg cacgcttcat ttcaacttac
    20521 aaagaagaac gtgtacatgc tagcaaggtg cttccaaaac cagctgcctt caactttgaa
    20581 ggagacaagg ctgaattgat tgaaaagatc cgtcaagccc tttacttctc aaaaatcatt
    20641 tcatacgcac aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa ctggaacttg
    20701 ccatttgcag atatcgcatc tatctggcgt gatggctgta tcatccgttc tcgtttcttg
    20761 caaaagatta cagatgctta caaccgtgat gcagaccttg ccaaccttct tttggatgaa
    20821 tacttcttgg atgttactgc taagtaccaa caagcagtac gtgatatcgt agctcttgcg
    20881 gttcaagctg gtgtgccagt gccaactttc tcagcagcta ttacttactt tgatagctac
    20941 cgttcagctg accttccagc taacttgatc caagcacaac gtgactactt tggtgctcac
    21001 acttaccaac gtaaagacaa agaaggaacc ttccactact cttggtatga cgaaaaataa
    21061 gtaggtcagc catggggaaa cggattttat tacttgagaa agaacgaaat ctagctcatt
    21121 ttttaagttt ggaactccag aaagagcagt atcgggttga tctggtagag gaggggcaaa
    21181 aagccctctc catggctctt cagacagact atgatttgat tttattgaac gttaatctgg
    21241 gagatatgat ggctcaggat tttgcagaaa aattgagtcg aactaaacct gcctcagtca
    21301 tcatgatttt agatcattgg gaagacttgc aagaagagct ggaagttgtt cagcgttttg
    21361 cagtttcata catctataag ccagtcctca tcgaaaatct ggtagcgcgt atttcggcga
    21421 tcttccgagg tcgggacttc attgatcaac actgtagtct gatgaaagtt ccaaggacct
    21481 accgcaatct taggatagat gttgaacatc acacggttta tcgtggtgaa gagatgattg
    21541 ctctgacccg ccgtgagtat gaccttttgg cgacacttat gggaagcaag aaagtattga
    21601 ctcgtgagca attgttggaa agtgtttgga agtatgaaag tgcgaccgag acaaatatcg
    21661 tagatgtcta tatccgctat ctacggagca agcttgatgt taaaggccaa aaaagctaca
    21721 ttaaaactgt gcgtggtgtt ggatatacca tgcaagaata gaaggctctt tgtcaactgt
    21781 agtgggttga agtcagctaa gcttgagaaa ggacaaattt cgtcctttct tttttgatgt
    21841 tcagagcgat aaaaatccgt tttttgaagt tttcaaagtt tcgaaaacca aaggcattgc
    21901 gtttgataag tttgatgaga ttattggtcg cttccaattt ggcgtttgaa tagggtagtt
    21961 gaagggcgtt gacgattttc tctttgttct ttagaaaggt tttaaagaca gtctgaaaaa
    22021 taggatgaat ctgcttcaga ttgtcctcaa tgagtccgaa aaatttctca gggtctttgt
    22081 tctgaaagtg aaaaagtaag agttgataga tctgatagtg gtgtttcaag tcttctgaat
    22141 agcttaaaat cttgtcaaga atttctttat ttgttaagtg catgcgaaaa gtagggcgat
    22201 aaaaacgttt atcgctga
//
LOCUS       DAVKZB010000023        21709 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKZB010000023 DAVKZB010000000
VERSION     DAVKZB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.023"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            87..2309
                     /gene="recJ"
                     /locus_tag="VXA80_000792"
     CDS             87..2309
                     /gene="recJ"
                     /locus_tag="VXA80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV0353323.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2484..4145
                     /locus_tag="VXA80_000793"
     CDS             2484..4145
                     /locus_tag="VXA80_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0353324.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4214..4993
                     /locus_tag="VXA80_000794"
     CDS             4214..4993
                     /locus_tag="VXA80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0353325.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKQALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5105..6325
                     /gene="murM"
                     /locus_tag="VXA80_000795"
     CDS             5105..6325
                     /gene="murM"
                     /locus_tag="VXA80_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV0353326.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6330..7562
                     /gene="murN"
                     /locus_tag="VXA80_000796"
     CDS             6330..7562
                     /gene="murN"
                     /locus_tag="VXA80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV0353327.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7641..8342
                     /locus_tag="VXA80_000797"
     CDS             7641..8342
                     /locus_tag="VXA80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV0353328.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8382..10229
                     /gene="uvrC"
                     /locus_tag="VXA80_000798"
     CDS             8382..10229
                     /gene="uvrC"
                     /locus_tag="VXA80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV0353329.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10219..11052
                     /locus_tag="VXA80_000799"
     CDS             10219..11052
                     /locus_tag="VXA80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0353330.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGP
                     GANDLVALLKELPITASVRGNWDDCVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDFFDF
                     L"
     gene            complement(11234..12034)
                     /locus_tag="VXA80_000800"
     CDS             complement(11234..12034)
                     /locus_tag="VXA80_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353331.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12205..12810
                     /locus_tag="VXA80_000801"
     CDS             12205..12810
                     /locus_tag="VXA80_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV0353332.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12823..14223
                     /gene="pepV"
                     /locus_tag="VXA80_000802"
     CDS             12823..14223
                     /gene="pepV"
                     /locus_tag="VXA80_000802"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV0353333.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14277..14855
                     /locus_tag="VXA80_000803"
     CDS             14277..14855
                     /locus_tag="VXA80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV0353334.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15015..15167
                     /locus_tag="VXA80_000804"
     CDS             15015..15167
                     /locus_tag="VXA80_000804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0353335.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15210..15301
                     /locus_tag="VXA80_000805"
                     /pseudo
     CDS             15210..15301
                     /locus_tag="VXA80_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            15472..16797
                     /gene="brnQ"
                     /locus_tag="VXA80_000806"
     CDS             15472..16797
                     /gene="brnQ"
                     /locus_tag="VXA80_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV0353336.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16895..17932
                     /locus_tag="VXA80_000807"
     CDS             16895..17932
                     /locus_tag="VXA80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV0353337.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSEAVS"
     gene            17934..18395
                     /locus_tag="VXA80_000808"
     CDS             17934..18395
                     /locus_tag="VXA80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0353338.1"
                     /translation="MCLICQRIELIKAGENPYFVKELETGYVVIGDHQYFKGYTLFLA
                     KEHVTELHQMENSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGNMKGHGLNGRGPVWWVPWEEMAAEDCQVQSPEREEMIKILSHELEKHLA"
     gene            18413..19129
                     /locus_tag="VXA80_000809"
     CDS             18413..19129
                     /locus_tag="VXA80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV0353339.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAAGYPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(19169..19306)
                     /locus_tag="VXA80_000810"
     CDS             complement(19169..19306)
                     /locus_tag="VXA80_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353340.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDVVS
                     R"
     gene            19438..19863
                     /gene="rplK"
                     /locus_tag="VXA80_000811"
     CDS             19438..19863
                     /gene="rplK"
                     /locus_tag="VXA80_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV0353341.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20071..20760
                     /gene="rplA"
                     /locus_tag="VXA80_000812"
     CDS             20071..20760
                     /gene="rplA"
                     /locus_tag="VXA80_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV0353342.1"
                     /translation="MAKKSKQLRVALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(21469..21549)
                     /locus_tag="VXA80_000813"
                     /pseudo
     CDS             complement(21469..21549)
                     /locus_tag="VXA80_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         6484 a         4023 c         5031 g         6171 t
ORIGIN      
        1 gttataaaaa agcaatctgg atagagaaaa cgtctaaatc atgttataat gaagcaatag
       61 aattcttaga aagagtggat gtctttttga taacacctac ttatgaatgg cagtttgccc
      121 tgcaggtaga agatgcggat tttacaaaga tagccaagaa ggctggactg ggtcctgagg
      181 tggctcggtt attgtttgag agagggattc aggaccaaga aagtctgaag aagtttttag
      241 aaccttcctt ggaggactta catgatgctt atctgctcca tgatatggac aaggcagtgg
      301 agcggattcg tcaggctatt gaagaagggg aaaatattct tgtttatgga gactatgatg
      361 cggatggcat gacttcggct tctattgtga aggaaagttt ggaacaactt ggtgctgagt
      421 gccgagttta cctgccaaat cgttttaccg atggctatgg ccctaatgct agtgtttata
      481 aatactttat cgagcaagaa gggatttcct tgattgtgac ggtggacaat ggggttgctg
      541 gtcatgaggc tattgcattg gctcagtcta tgggagtaga tgtcattgtg acagaccatc
      601 attccatgcc tgaaaccctg ccagatgctt atgctattgt ccatcctgaa catccagatg
      661 cggattatcc ttttaaatat ttggctggtt gtggagttgc tttcaagttg gcttgtgccc
      721 tgttagaaga agtgcaagtg gaattgcttg atttggtcgc tattggaact attgcagata
      781 tggtgagtct gacggatgaa aatcgtatct tagttcaata tggtctggaa atgttgggtc
      841 atacccagcg cattggtctg caagaaatgc tggacatggc tgggattgct gccaacgaag
      901 taacagaaga aacggttggt ttccagattg ctcctcgttt gaatgccttg ggccgcttgg
      961 atgatcccaa tcctgccatt gatttgttga ctggatttga tgatgaggaa gcgcatgaga
     1021 ttgcccttat gattcaccag aaaaacgaag agcgcaagga aatcgttcag tctatctatg
     1081 aagaagccaa gaccatggtg gatcctgaga agaaggttca ggtcttggcc aaggaaggct
     1141 ggaatcctgg ggttctagga atcgtggctg gtcgtttatt ggaagaattg ggacagacag
     1201 tcattgttct taatatagaa gacggtcgtg ccaagggcag tgctcgtagt gtggaagcgg
     1261 tcgatatttt tgaagctctg gatccccatc gagacctctt catcgccttt ggaggtcatg
     1321 caggtgcagc gggtatgacg ctggaagttg agcaactctc agatttatct caggttttgg
     1381 aagattatgt tcgtgaaaaa ggtgcagatg ctggtggcaa gaataagtta aacctagatg
     1441 aagagttgga tttggaggca cttagcttgg aaacggtcaa aagttttgaa cgtttagctc
     1501 cttttggaat ggataatcag aaacctattt tttatatcaa gaattttcag gtcgaaagtg
     1561 ctcgtactat gggggcaggt aatgcccatc taaagctgaa aatttccaag ggtgaggcga
     1621 gttttgaagt ggtagccttt ggtcaaggca gatgggcgac agagttttct caaaccaaga
     1681 atctagagtt agcggttaaa ttgtctgtca accaatggaa tggccaaact gccctccagt
     1741 tgatgatggt ggatgcgcga gtggaaggtg ttcaactttt taacattcgt ggaaaaaatg
     1801 cagtcttgcc agaaggtgtt ccagtcttgg attttcctgg agaactgcca aatcttgcgg
     1861 ctagtgaagc tgttgtcgta aaaaacattc cagaggatat tactcagctg aagaccattt
     1921 ttcaggaaca gcatttctct gctgtctatt tcaaaaatga tattgacaag gcctattatc
     1981 tgacaggtta tgggactaga gatcagtttg ccaaattgta caagactatt taccagttcc
     2041 cagagtttga tattcgctac aagctgaaag atttggctgc atatcttaat attcaacaaa
     2101 tcttgctggt caagatgatt caagtatttg aagaactagg ctttgtgacg ataaaagatg
     2161 gtgtgatgac agtcaataaa gaggcgccaa agcgggagat aggagaaagt caaatttacc
     2221 aaaatctcaa acaaaccgtt aaagaccaag aaatgatggc gctgggtacg gtgcaagaaa
     2281 tttatgattt tttgatggaa aaagagtaga agttaggaaa gagttgggaa atcaactctt
     2341 ttttgaaaac agaccttcat tttgaaaatc atcaaaaaaa tggtataatg gtaggaaaag
     2401 attcggctga aagtgttaga acttttagaa taagagggta gagttgccct ataatcaaga
     2461 taaactaaga ttttggagga aaaatgagta atatcagttt aacaacactt ggtggtgtgc
     2521 gtgagaatgg aaaaaatatg tacattgctg aaattggaga gtccattttt gttttgaatg
     2581 tagggttaaa atatcctgaa aatgaacaat taggggtcga tgtggtgatt ccaaacatgg
     2641 attacctttt tgaaaatagc gaccgtattg ctggggtttt cttgacccac gggcatgcgg
     2701 atgccattgg tgctctacct tatctcttgg cagaggctaa agttcctgta tttgggtctg
     2761 agttgaccat tgagttggca aagctctttg tcaaaggaaa tgatgccgtt aagaaattta
     2821 atgatttcca tgtcattgat gagaatacgg agattgattt tggtgggaca gtggtttcct
     2881 tcttccctac gacttactcc gttccagaga gtctgggaat tgtcttgaag acatcggagg
     2941 gaagcatcgt ttatacaggt gacttcaaat ttgaccaaac ggctagtgaa tcttatgcaa
     3001 ctgattttgc tcgtttggca gagattggtc gtgacggcgt cctggctctc ctcagtgatt
     3061 cggccaatgc agacagcaat attcaggtgg ctagtgaaag tgaagttagg gatgaaatta
     3121 cccaaactat tgctgactgg gaaggtcgta tcatcgttgc agctgtttcc agtaatcttt
     3181 ctcgtattca gcagattttt gacgctgcgg ataaaacagg tcgacgtatc gtcttgacag
     3241 gatttgatat tgaaaatatc gtccgcacag cgattcgtct taagaagttg tctttagcca
     3301 acgaaattct cttgattaag cctaaagata tgtctcgctt tgaagaccat gagttgatta
     3361 ttcttgagac aggtcgtatg ggtgaaccta tcaatggact tcgtaagatg tcgattggtc
     3421 gccatcgtta tgtagaaatc aaggatgggg acctggtcta tattgctacg gctccgtcta
     3481 ttgctaaaga agcctttgtt gcgcgtgtgg aaaatatgat ttatcaggca ggtggggttg
     3541 tcaaattgat tacccaaagt ttacatgtat cagggcacgg aaatgtgcgt gatttgcagc
     3601 tgatgatcaa tcttttgcaa cctaagtacc tcttccctgt ccaaggggag tatcgtgagt
     3661 tggatgctca cgctaaggct gccatggcag ttgggatgtt gccagaacgc atcttcattc
     3721 ctaaaaaggg gacgaccatg gcttacgaga atggagactt tgttccagct ggatcggttt
     3781 cagcaggaga tatcttaatt gatgggaatg ccattggtga tgttggaaat gttgttcttc
     3841 gtgaccgtaa ggtcttgtca gaggatggaa ttttcatcgt ggctattaca gtcaaccgtc
     3901 gtgagaagaa aattgtggct agggctcgtg ttcacacgcg tggatttgtt tatctcaaga
     3961 agagtcgcga tattctccgt gaaagttcag aattgattaa ccaaacggta gaagattatc
     4021 ttcaaggaga tgactttgac tgggcagatc ttaaagggaa ggttcgagat aatttgacca
     4081 agtatctctt tgaccaaacc aagcgtcgtc cagctatttt accagtagtc atggaagcaa
     4141 aataatcgtt gaaataaaca gagagaaagt cgagtttcgg ctttttctta tagaaaaata
     4201 gaaggagaaa atcatggcag taatgaaaat cgagtattac tcacaagtat tggatatgga
     4261 gtggggggtg aatgtcctct accctgatgc caatcgagtg gaagaaccag agtgtgaaga
     4321 tattcccgtc ttgtaccttt tgcacgggat gtctggaaat cataatagtt ggcttaagcg
     4381 gaccaatgta gaacgcttgc ttcgaggaac taatctcatc gttgttatgc ccaataccag
     4441 caatggttgg tacaccgata cccagtatgg ttttgactac tacacggctc tagcagagga
     4501 attgccacag gttctgaaac gcttcttccc taatatgact agcaagcgtg aaaagacctt
     4561 tatcgctggt ctttctatgg gaggttacgg ctgcttcaaa caggctcttg cgacaaatcg
     4621 tttttctcat gcagctagtt tttcaggtgc cctcagcttt caaaactttt ctcctgaaag
     4681 tcaaaatctg ggaagtccag cctactggag aggtgttttt ggagagatta gagactggac
     4741 aactagtccc tattctcttg aaagtctggc taaaaaatcg gataaaaaga ccaaactttg
     4801 ggcgtggtgt ggcgaacagg atttcttgta cgaagccaat aatctcgcag tgaaaaatct
     4861 caaaaaacta ggttttgatg tgacctatag ccatagcgct ggaactcacg agtggtacta
     4921 ctgggaaaaa caattggaag tttttttaac aaccctacca attgatttca aattagaaga
     4981 gagactgact tagtttgaac ttcagcatag ggggagtaga actaaaataa aatatgtttt
     5041 cactagactt ttcaaacgaa agtagtagaa tagtaataaa atactggagg aaagagagta
     5101 ggaaatgtac cgttatcaaa ttggcattcc cacattagaa tatgatcagt ttgtcaaaga
     5161 acatgaatta gccaatgtat tacaaagtag tgcttgggag gaagttaagt ctaattggca
     5221 acatgagaag tttggtgttt acagggaaga aaaattactg gcgacagcta gtattttgat
     5281 tagaactctt ccgctaggct ataaaatgtt ttacatccca agaggaccta tattggatta
     5341 tggggataaa gaactcttga attttgtcat tcagtctatt aagtcctatg ctcgcagtaa
     5401 gagagcggtt tttgtgactt ttgacccaag tatttgccta tctcaaagtt taatcaatca
     5461 ggaaaagaca gaatttcctg aaaatctggc tattattgat agtttgcaac aaatgggagt
     5521 aaggtggtca ggaaaaacgg aggaaatggg agacaccatt caacctcgta ttcaggcgaa
     5581 aatatacaag gaaaattttg aagaagataa actttccaag tcaacaaaac aggctattcg
     5641 aacagcacga aacaaagggc ttgagattca atatggtgga ctggaactat tagattcatt
     5701 ttcggagttg atgaaaaaaa ctgagaagcg aaaagagatt catttgagga atgaagccta
     5761 ttataaaaaa ttgttagata attttaagga caaggcctat atcaccttgg ccaccttgga
     5821 tgtttctaaa cgttcgcaag aattagaaga acagttagcg aaaaatagag ccttggaaga
     5881 gacgtttact gagtcgactc gaatttcaaa agtagaagcg cagaagaagg aaaaagaacg
     5941 tttgttagag gaattgacct tcttgcagga atatatagat gtaggtcaag cgagagttcc
     6001 tttagcggct actttgagtt tggaatttgg tactacctct gtcaatatat atgctggtat
     6061 ggatgatgat tttaaacgtt acaatgcacc aattttaaca tggtatgaaa cggctcgcta
     6121 tgcctttgag cgaggtatgg tttggcaaaa tttaggtggt gttgaaaact ctctcaatgg
     6181 tggactttat cattttaagg aaaaatttaa tccaacgatt gaagaatact tgggtgaatt
     6241 tacaatgccc actcatcctc tctatcctct gttaagactt gctcttgatt tccgtaaaac
     6301 attaagaaaa aaacatagaa agtaagtata tggcactaac aacactcacg aaagaagagt
     6361 ttcagactta ttctgatcag gtttcttctc gttcctttat gcaatctgtc cagatggggg
     6421 atttgctaga aaaaagaggg gctcgaattg tttatcttgc tttgaaacaa gaaggagaaa
     6481 ttcaagttgc agctctggtt tatagcttgc ccatgctggg tggtctgcat atggaactca
     6541 attcggggcc gatttatacc caacaagatg ctcttccagt tttttatgca gagttaaaag
     6601 aatatgccaa gcaaaatggt gtattagagt tgcttgtaaa accttatgaa acttatcaaa
     6661 cttttgatag ccaaggtaac tcaatagatg ctgagaaaaa aagtattatt caaggtttga
     6721 ctgatttagg ttatcaattt gatggcttaa caacaggtta cccaggtgga gaaccagatt
     6781 ggttatacta taaagattta actgaattaa ctgaaaagag tttgcttaaa agttttagca
     6841 aaaagggtaa acccttggtg aaaaaggctg aaacctttgg cattcggttg aaaaagttaa
     6901 aacgtgaaga actatcgatt tttaagaata taacaaaaga aacctctgaa cgtagagaat
     6961 atagtgataa aagtttagaa tattatgagc atttttatga tacttttgga gaacaagcgg
     7021 agtttctcat agcaagctta aatttttcgg actatatgag caaattgcaa ggtgaacaaa
     7081 gtaaactaga agaaaacttg gacaagttgc gacttgattt gagtaaaaat cctcattctg
     7141 agaaaaaaca aaatcaactg agagaatatt ctagtcaatt tgaaacgttt gaagttcgaa
     7201 aagcagaagc gcgagacttg attgaaaaat atggagaaga agatattgtt ttagctggga
     7261 gtttatttgt ttatatgcct caggaaacga cttatctctt tagtggttcc tacactgagt
     7321 ttaataagtt ctatgcccct gccctgcttc aaaaatatgt tatgttggaa agcataaaac
     7381 gtggaatacc taaatacaac ttcctaggca ttcaagggat ttttgatgga agtgatggtg
     7441 ttttgcgttt taaacagaat tttaatggct atattgtacg caaagcgggt actttccgtt
     7501 accatccatc gcctttaaaa tacaaagcta tccagttact caaaaaaata gtaggacgtt
     7561 aagatgaaaa agtcagtatt tagatttctt ttagcttctt ttagtaaaat aattcttatt
     7621 tgctagaaag gtggagagac atgcgctggc tttttcgttt gataggggct ttcttttctt
     7681 ttgtgtggcg tttgttttgg cgtctggttt ggatagttgt gctcttatgt gtgcttgctt
     7741 tcggacttct ctggtatctg aacggggatt ttcaaggagc gctaaagcaa gcagaacggt
     7801 cagtaaaaat tggtcaacaa agtattgacc aatgggagaa aacagggcaa ctgcctaagt
     7861 taagccagac agatagtcac cagcattctg aaggaaggtg ggcacaggcc tctgctcgta
     7921 tttacctgga tccgcagatg gattcacgct ttcaagaggc ttatttagaa gcaatccaga
     7981 actggaatca aactggtgct tttaactttg aactcgtgac tgagtctagt aaggcggata
     8041 ttacggctac ggagatgaac gacggaggca ctcctgtggc aggagaggcg gaaagtcaaa
     8101 ctaatctctt aacagggcaa ttcttgtccg taacggtgcg gttgaatcat tattatttgt
     8161 ccaatccata ctatggctac tcctatgaac gccttgtcca tacggcagaa catgagttag
     8221 gtcatgcgat tggcttggac catacagatg agaagtctgt catgcaacca gcaggttcct
     8281 tttatggtat ccaggaagag gatgttgcaa acctccgaaa aatatatgag actagtgagt
     8341 agggtactat ctttccctac tttttttgct ataatggaac tatgaacaac ttgattaaat
     8401 caaaactaga gctcttgccg accagccctg gttgctacat tcataaggat aaaaatggca
     8461 ccattatcta tgtaggaaag gctaaaaatc tgcgtaatcg agtacggtcc tattttcgtg
     8521 gaagtcatga taccaagaca gaggctctgg tgtctgaaat tgtggatttt gaatttattg
     8581 ttacggagtc taatattgag gcacttctcc tagaaatcaa cctgatcaag gaaaacaagc
     8641 ccaagtacaa tatcatgctc aaggatgaca agtcctatcc tttcatcaaa atcaccaatg
     8701 agcgctatcc acgcttgatt atcactcgtc aggtcaaaaa ggacggaggt ctttattttg
     8761 gaccctatcc cgatgtgggg gcagccaatg aaatcaagcg gttgctggat cggatattcc
     8821 cttttcgtaa gtgtaccaac ccgccctcta aggtctgttt ttattaccat atcggtcaat
     8881 gcatggccca caccatctgt aagaaggatg agacttattt caagtctatg gcccaggagg
     8941 tttctgattt tctgaaaggg caggataaca aaatcattga tgagctaaag ggcaaaatgg
     9001 cagcggcagc ccagactatg gagtttgaac gtgcggcgga ataccgtgac ctgattcagg
     9061 ctattggaac gcttcgaacc aagcaacggg tcatggctaa ggatctccaa aatcgcgatg
     9121 tctttggcta ctatgtggat aagggctgga tgtgtgtgca ggttttcttt gtccgtcagg
     9181 gtaagctcat cgagcgcgat gtcaatctct tcccctactt caatgatcca gatgaggatt
     9241 ttttgaccta tgtaggacaa ttctatcaag aaaaatctca cctagttccc aatgaggtac
     9301 tgattccgca ggatattgac gaagaagctg tcaaggcttt ggtggatacc aagattctca
     9361 aaccccaacg tggagagaaa aaacagctgg tcaatctagc cataaaaaat gcacgtgtta
     9421 gtctggagca gaaatttaac ctgctagaga aatctgtcga gaagacccaa ggagctattg
     9481 aaaatctagg acgtttgctc caaatcccga ccccagtacg tatcgagtcc ttcgataact
     9541 ctaatatcat gggaactagc cctgtttcgg ctatggtggt ctttgtcaac ggtaaaccga
     9601 gtaagaagga ttaccgtaag tacaagataa aaacggttgt tggaccagac gactatgcca
     9661 gcatgagaga ggtcattcgc agacgctatg gtcgagtaca gcgtgaggct ttgactcctc
     9721 cagatttgat tgtgattgat ggggggcaag gtcaagtcaa tattgctaag caagtcatcc
     9781 aagaagaact gggcttggat attccaattg caggtctgca aaagaatgat aagcaccaaa
     9841 cccatgaatt gctctttgga gatccgctgg aagtggtgga cttgtctcgc aattctcagg
     9901 aatttttcct cctccaacgc atccaagatg aggtccaccg ctttgccatt accttccacc
     9961 gccaactgcg ctccaaaaat tctttctcat ctcaattgga tgggattgat ggtctgggac
    10021 ctaaacgcaa gcaaaatctc atgaggcact tcaagtctct gaccaaaatc aaggaagcca
    10081 gtgtggatga gattgtcgaa gttggagtac caagagcagt agcagaggct gtgcaaacca
    10141 agttgaaccc gcaggaaaca gaaatcttgc ttcaagtggc ggaagaaaga gtagattacc
    10201 aaacggaagg aaaccacaat aaaccataaa atcgcaattt tatcagatat tcatggcaat
    10261 gcgacggcgc tagaagcagt gattgcagat gctaaaactc aaggagccag tgaatattgg
    10321 ctcatgggag acattttcct ccctggtcca ggtgcaaatg acttggtcgc tctgttaaag
    10381 gaacttccta tcacggcaag tgttcgaggc aattgggatg attgtgtcct tgaggcttta
    10441 gatgggcaat atggcttaga agacccacag gaagttcagc tcttgcgtat gacacagtat
    10501 ttgatggagc gaatggatcc tgcaacgatt gtctggctac gaagcttgcc tttgctggaa
    10561 aagaaagaaa ttgacggatt gcgcttttct atctctcata atttacctga caaaaactat
    10621 ggtggtgact tgctagttga gaatgataca gagaaatttg accaactact agatgcggaa
    10681 acggacgtgg cagtttatgg tcatgttcac aagcagttgc ttcgttatgg aagtcaaggg
    10741 caacaaatca tcaatccagg gtcgattggc atgccctatt ttaattggga ggcgttaaaa
    10801 aatcaccgtg cccagtatgc cgtgatagaa gttgaagatg gagaattact caatatccaa
    10861 tttcgtaaag ttgcttatga ttacgaagct gagttagaat tggccaagtc caaggggctt
    10921 ccctttatcg aaatgtatga agaactgcgt cgtgaagata actatcaggg gcacaatctg
    10981 gaattattag ccagcttaat agaaaagcat gggtatgtag aggatgtgaa ggattttttt
    11041 gattttttgt aagagtttcc taaaatagcc aatgcaaact aaaaaagcga ttggctggtc
    11101 caatcgcttt tagtatatct tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    11161 cgtaggttgc tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg tttgaagaga
    11221 ttttcgaaga gtgttattgt aactgagatt gatctgggag gtaagaacca cctagatagg
    11281 tattgctgag tttttcaagg gttccgtctt gatagagttc tttgagcgct ttatcaaatt
    11341 gctctttaaa ctctttttgg tcgcttgaga aaacgatata gttgctgggg ctatctgcag
    11401 aaggtaaatc aacgactgag aggtctaaac cacggtcctt gataatcttt tgaacggata
    11461 ccttgtcaaa aactaggaaa tcaaactctc cgttagcaag gtctaggatt cgtttaccaa
    11521 tatcctcacc agaaaaatta attgtagcgg gattatcagt gtgtttctga ttccagttat
    11581 tgatgaattg agcgttagaa gttccggtat cctcttgtgt tgttttacca gcgatctggt
    11641 caagagaagt caaaggattt ttcttgttgc tgacaaggac gaggggattg ttggaaattg
    11701 gaagcgagta aaggtatttt tcagcgcgct cttttgtgta actcaagtta ttggccgcag
    11761 cctgatagtg accagaatca agtcctggga agatgctctc ccaggcggtt ctttggaatt
    11821 gaatctcgta gtcgctgagt ttttcatcta ctgcctttaa aacttcgata tcgaagcctg
    11881 tcagattgcc cttgtcttcg tagtcaaatg gtggcacgtc gccagctgta gcaaggacga
    11941 ttgtcttttg agcgctagtc tctttgggtg tagcttgatt cccacaggca accaaaaatg
    12001 gtaggatagc tagtaatagg ctaaattttt tcatactgtc tccattcaaa tgtaaagtac
    12061 ttgctagttt atcagaaact ttcctgataa accaatatat atcttttatg tctgtgatag
    12121 aaaaacttaa tcatgaaaaa tccctgcaag ctctttcaaa ttatggtaga atggaagcag
    12181 tagaattaga aaaggaaatc gattatgaaa tttcttgaat taaataaaaa acgtcatgcg
    12241 actaagcatt ttactgataa gcctgttgat cccaaagatg tgcgtacggc tatcgaaatt
    12301 gcaaccttgg cgccaagcgc ccacaacagc cagccttgga aatttgtggt ggtacgtgag
    12361 aaaaatgctg aactggcaaa gttagcttat ggttccaatt ttgaacaggt atcatcagcg
    12421 cctgtaacca ttgccttgtt tacagatacg gacttagcca aacgtgctcg taagattgcc
    12481 cgtgttggtg gtgctaataa cttttctgaa gagcaacttc aatattttat gaaaaatctg
    12541 ccagctgagt ttgcccgtta cagtgagcaa caagtcagcg actacctagc tctcaatgca
    12601 ggtttggttg ccatgaactt ggttcttgca ttgacagacc aaggaattgg ttctaacatt
    12661 attcttggtt ttgacaaatc aaaagttaat gaagttttgg aaatcgaaga ccgtttccgc
    12721 ccagaactct tgatcacagt gggttataca gacgaaaaat tggaaccaag ctaccgcttg
    12781 ccagtagatg aaatcatcga gaaaagatag aaagaagaaa aaatgacagc aattgatttt
    12841 acagcagaag tagaaaaacg caaagaagac ctcttggctg acttgtttag ccttttggaa
    12901 atcaattcag aacgtgatga cagcaaggct gatgcccagc atccatttgg gcctggtcca
    12961 gtaaaagcct tggagaaatt ccttgaaatc gcagaccgcg atggctaccc aactaagaat
    13021 gttgataact atgcaggaca ttttgagttt ggtgatggag aagaagttct cggaatcttt
    13081 gcccatatgg atgtggtgcc tgctggtagc ggttgggaca cagaccctta cacaccaact
    13141 atcaaagatg gtcgccttta tgcgcgcggg gcttcggacg ataagggtcc tacaacagct
    13201 tgttactatg gtttgaaaat catcaaagaa ttgggtcttc caacttctaa gaaagttcgc
    13261 ttcatcgttg gaacagacga agaatcaggc tgggcagaca tggactacta ctttgagcac
    13321 gtaggacttg ccaaaccaga tttcggtttc tcaccagatg ctgaatttcc aatcatcaat
    13381 ggtgaaaaag gaaatatcac ggaatacctc cactttgcag gagaaaatac aggtgttgcc
    13441 cgtcttcaca gctttacagg tggtttacgt gaaaatatgg taccagaatc agcaacagca
    13501 gtcgtttcag gtgacttggc tgacttgcaa gctaaactag atgcctttgt tgcagaacac
    13561 aaacttagag gagaactcca agaagaagct ggcaaataca aggtgacgat cattggtaaa
    13621 tcagcccacg gtgctatgcc tgcttcaggt gtcaatggcg caacttacct tgccctcttc
    13681 ctcagccagt ttggctttgc tggtccagcc aaagactacc ttgacatcgc aggtaaaatt
    13741 ctcttgaacg atcatgaggg tgaaaatctt aagattgctc atgtggatga aaagatgggt
    13801 gctctttcta tgaatgccgg cgttttccgc ttcgatgaaa caagtgctga taataccatt
    13861 gccctcaaca tccgctatcc aaaaggaaca agtccagaac aaatcaagtc aatccttgaa
    13921 aacttgccag ttgtttctgt tagcctgtct gaacacggtc acacgcctca ctatgtgcca
    13981 atggaagatc cacttgtgca aaccttgttg aatatctatg aaaaacaaac tggccttaaa
    14041 ggtcatgaac aagtcatcgg tggtggaacc tttggtcgct tgctagaacg cggagttgcc
    14101 tacggtgcta tgttcccaga ctcgattgat accatgcacc aagccaatga atttatcgcc
    14161 ttggatgatc ttttccgagc agcagcaatt tatgccgaag ctatttacga attgatcaaa
    14221 taaaacgata gaagtctgag atcttatgct tggacttctt tttggaggga aagtagatgt
    14281 ctcaaatcga aagaatcaaa caggctatca tggcggattc acagaatgcc agctatacag
    14341 agcgtggcat tgagcctctc tttgcagcgc caaaaactgc tcgcatcaat atcatcggtc
    14401 aggctccggg acttaaaact caagaagcag gcctttactg gaaagataaa agtggtgacc
    14461 gcttgcggga ctggctaggt gtggatgaag atacctttta caattcaggt tattttgctg
    14521 ttttgcctat ggatttctac tttccaggac atggcaagtc gggtgatctt ccgcctcgta
    14581 caggttttgc agaaaaatgg catccgcagc tcttacagga attgcctgat attcagttaa
    14641 ccctcttgat tgggcaatat gcccaagcct actatttaca ggagaaaatc agtgggaagg
    14701 taacggagag ggtgaaacac tataaagact atctgccagc ctattttccg ctagttcacc
    14761 catcaccacg aaatcaaatc tggatggcca aaaatccttg gtttgaggca gaagtagtgc
    14821 cagatttgaa aaaaagaatt aaaaccattt tatagtcaat gaaaatcaaa gagcaaacta
    14881 ggaagctagt cgtaggctgc tcaaagtaca gctttgaggt tgcagataaa actgacgaag
    14941 tcggtaacat acgcacggta aggcgacgct gacgtggttt gaagagattt tcgaagagta
    15001 ttagaagaaa aagaatgaaa gaaatagcct ttgacgcatt ttaccagctt taccaaaacg
    15061 accagctttc tctagtggat gtgagagaag tggatgagtt tgcagctctt catttagaag
    15121 gtgcccacaa cctaccgctt agtcaattgg ctgatagtta tgattaattg gacaaagatc
    15181 gcttgcatta tattatttgc aaatctggaa tgagatcggc gcgtgcttgc caattctatt
    15241 agaacaaggt tataatgtta tcaatgtcca gggtggcatg ttagcctttg aagaacttta
    15301 aaattttgca tttctcctac ttggtgtgga ctgggtagga gagttttatt tttagataat
    15361 tcttattttt aagaaaattg aaaacattta atatttgcct cgtgatgctt ttttcagact
    15421 cctaatcgtg gtatactagg tcagtatttt ataaatatga aggagatttt tatggctaaa
    15481 aaaggtaccc taacaggttt gctcctgttt ggaatatttt ttggtgcggg gaacttgatt
    15541 tttccgcctt ctctaggtgc tctatctgga gaacattttc ttcctgccat cgcaggtttt
    15601 gtcttttcag gcgttggtat cgccgtcttg acccttatta ttggaacgct aaatcctaaa
    15661 gggtatatct acgagatttc aacgaagata gcgccttggt ttgcgactct ttacctctca
    15721 gttctttact tgtcaatcgg tccattcttt gctaccccac gtactgctac aacagcttac
    15781 gaagtaggga ttagccccct tttgtcggat gcaaataaag gacttggctt gattgtattt
    15841 acggttctgt attttgcggc agcctatttg atttcgctta atccatcaaa aatcttagac
    15901 cgcattggac gtattttaac gccagtcttt gcaattttga ttgttatctt ggtcgttctg
    15961 ggagctatca aatatggtgg aacaagtcct caagctgctt cagctgctta tcaagcttct
    16021 gcctttggta caggtttcct agaaggttat aataccttgg acgcccttgc ctcagtggcc
    16081 tttagtgtaa tcgcagttca aaccttgaaa caacttggat tttcaagtaa gaaagaatac
    16141 atttcaacta tttgggttgt tggtatcgtt gttgcccttg ccttcagcgc tctttacatc
    16201 ggtttaggtt ttcttggaaa tcatttccca gtaccagctg aagcgatgaa gggtggaaca
    16261 ccaggtgttt acatcttgtc acaagccact caagaaatct ttggctcaac agctcaactc
    16321 ttccttgcag ctatggttac cgtaacctgc ttcacaacga ctgttggttt gattgtgtca
    16381 acagctgagt tctttaatga gcgcttccca caaatcagct acaaggttta tgcgacagcc
    16441 tttaccttga ttggatttgc tattgccaat ttgggtcttg atgcgattat caagtactca
    16501 attccagtac tggttatctt gtacccaatc acgattgcta tcgttatgat tgtcattgtc
    16561 aacaaatttg ttgccctttc aaaaccaggt atgcagttga caattgctgt ggttacagtt
    16621 attgccattg caagcgtact aggaagctcg tttaaggttg agtttcttgc aaatcttgtt
    16681 agcgttcttc cttttgccaa ggcatctctc ccatggttgg tgccagccat tgttggaatc
    16741 ttgctctcat tggttctacc aaacaagcaa gaaagcgatg tttttgaaat ggaataatca
    16801 cttaaatcac ttttgtagcc aagtctacag gagtgatttt ctttttttat ccgatgataa
    16861 atgtgttata ataggtagcg aaagaggtga agaaatgaat caaacagtag aatatatcaa
    16921 agaactgaca gccatcgcat cgccaacggg ctttactcgt gaaatttcga attatttagt
    16981 caagactcta gaaggttttg gttaccagcc agttcgcaca gctaagggtg gtgtcaatgt
    17041 gaccatcaaa ggtcaaaatg atgagcaaca tcgctatgtg actgctcatg tggatacgct
    17101 gggtgctatt gttcgtgctg tcaaaccaga tggtcgtctt aaattggacc gtatcggtgg
    17161 ttttccttgg aacatgattg aaggtgaaaa ctgtaccgtt catgtggcta gcacaggtca
    17221 aaaggtatca ggaaccatcc tcatccacca aacttcttgc catgtctaca aggatgcagg
    17281 aactgcagag cgcacgcagg acaatatgga agtgcgtttg gatgtaaaag taagcaatga
    17341 aaaagaaact cgtgctcttg ggattgaggt cggtgatttt atcagcttcg atccacgaac
    17401 tgttgtgact gatacaggtt ttatcaagtc tcgtcatttg gatgataagg tcagcgcagc
    17461 gattttgctc aacctgcttc gtatttataa gaaagagaag attgaattgc cagtaacaac
    17521 tcattttgct ttttcagttt ttgaagaagt aggacatggt gctaattcta atattcctgc
    17581 tcaagtagtc gaatatctgg ctgtggatat gggagctatg ggggatgatc agcaaacaga
    17641 tgagtacaca gtgtccatct gtgtcaagga tgcttcaggt ccttatcact atgacttccg
    17701 tcaacatttg gtgaccttgg ctaaaaacca agatattcca ttcaagctgg atatctatcc
    17761 attttatggt tcggacgctt cagcggctat gtctgcaggt gcagaagtta aacacgctct
    17821 tcttggtgcg ggtatcgagt ctagtcattc ttatgagcgt actcatattg actcggtggt
    17881 cgcaacagag cgtatggtag atgcctatct caagagtgag gcggtatcat agaatgtgct
    17941 taatctgtca aagaattgaa ctcatcaaag ctggagaaaa tccctacttt gtcaaagaac
    18001 tagaaacggg ctatgtagtt attggagacc accaatactt taagggctat accttatttt
    18061 tagcaaaaga acatgttaca gaactccacc aaatggagaa ttctgtaaaa cttcgctttt
    18121 tagaggaaat gagtttggtc caagaagcag tcgccaaagc atttgaggct gaaaagatga
    18181 atattgaact cctaggaaat ggcgatgccc acgctcattg gcatcttttt ccgcgaagag
    18241 caggtaatat gaaagggcac ggacttaatg gccgtggccc agtctggtgg gtgccctggg
    18301 aagaaatggc agcagaagat tgccaagtgc aatcgccaga gcgggaagag atgattaaaa
    18361 tcttatctca tgaattggag aagcatttgg cctaagagag aggaagaaag aaatgaaaaa
    18421 aagatatgtc attttatctg gtttactagc attgactctt gcagcttgtt cgcaagaaaa
    18481 aacgaaagtt gaagaaaata ctcaaaagac ggagcaaagc agtcaaccgg aaggaactgt
    18541 aggcagcaaa tctcaagctt ctagtcagaa aaaggcagaa gttagcaata aagggagcta
    18601 ttatagtatt caagggaaat acgatgaaat tatcctagct aataaacgtt atcctttgtc
    18661 aaaagactac aatccaggag aaaatccaac agccaaggca gagttgctga aactcattgc
    18721 agcaatgcaa gcagctggct acccaatcag cgatcactac agtggtttta gaagttatga
    18781 aactcagacc aagctctatc aagattatgt caaccaagat ggaaaggcag cagctgaccg
    18841 ttactctgcc cgtcctggct atagcgaaca ccagacaggc ttggcctttg atgtgattgg
    18901 gactgatggt gatttggtga cagaagaaaa agcagcccaa tggctcttgg atcatgcagc
    18961 tgattatggc tttgttgtcc gttatctcaa aggcaaggaa aaggaaacag gctatatggc
    19021 tgaggaatgg cacctgcgtt atgtaggaaa agaagctaaa gaaattgctg caagtggtct
    19081 cagtttggaa gaatactatg gctttgaagg cggagactac gtcgattaat actcttcgaa
    19141 aatctcttca aaccacgtca gcgtcgcctt acctactgac tacgtcggtt ctattcacaa
    19201 cctcaaaaca gtgttttgag ctgacttcgt cagttttatc tgcaacctca aagctgtact
    19261 ttgagcagcc tgcggctagc ttcctagttt gctctttgat tttcattgag tacaaaaaat
    19321 aaacttttct cttgcaattc cagataaata gtgtataatg gatgggtatg tgaaaaacat
    19381 acttgtggga ggtaaaaatc tctaattacc gccaaaacca caaaggagga tttaaaaatg
    19441 gctaaaaaag tcgaaaaact tgtaaaattg caaatccctg ctggtaaagc tacaccagct
    19501 ccaccggttg gacctgctct tggtcaagct ggtatcaaca tcatgggatt cacaaaagag
    19561 ttcaacgctc gtacagctga ccaagctggt atgatcattc cagttgttat ctcagtttac
    19621 gaagataaat catttacttt cgttacgaaa acaccaccag ctgctgttct tttgaaaaaa
    19681 gctgcaggtg ttgaaaaagg atcaggtaca cctaataaaa ctaaagttgc tacagttact
    19741 cgtgcacaag tacaagaaat tgcagaaact aagatgccag atttgaacgc agcaaacgta
    19801 gagtctgcaa tgcgtatgat cgaaggtact gctcgttcta tgggattcac tgttgttgac
    19861 taatcaataa cacagtagaa aatctcacag cagtctatta gttgcttttc atactaggca
    19921 agtgactgag ccttgtactt gggtacagca agggagctta aggccgtagg aagagaaaaa
    19981 tagtagactg aaaacccgca agacttcatc atttcgagaa gtgacgtggg agatgaaaat
    20041 cgattgaacc acttacaagg agaatagaaa atggctaaaa aaagcaaaca acttcgtgtt
    20101 gctcttgaga aaatcgacag cacaaaagca tacagtgtag aagaagctgt agcacttgca
    20161 aaagaaacta actttgcaaa atttgacgca actgtagaag ttgcttacaa cttgaacatc
    20221 gacgttaaaa aagctgacca acaaatccgt ggagcaatgg tattgccaaa cggtactggt
    20281 aaaacttcac gtgttcttgt tttcgcacgt ggtgcaaaag ctgaagaagc aaaagctgct
    20341 ggtgcagact ttgttggtga agatgacctt gttgctaaaa tcaacgacgg ttggttggac
    20401 ttcgacgtag ttatcgctac acctgatatg atggctcttg ttggacgtct tggacgtgtc
    20461 cttggaccac gtaacttgat gccaaaccct aaaactggta ctgtaacaat ggatgttgct
    20521 aaagcagttg aagagtctaa aggtggtaaa atcacttacc gtgctgaccg tgcaggtaac
    20581 gttcaagcaa tcatcggtaa agtatcattt gaagctgaaa aattggttga aaactttaaa
    20641 gctttcaacg aaacaatcca aaaagcaaaa ccagctacag ctaaaggaac ttacgtaaca
    20701 aacttgacta tcacaactac tcaaggtgtt ggtatcaaag ttgatgtaaa ctcactttaa
    20761 tcagtagtta aagtaatgta aaaaagttga agacgctatg tctcaacttt ttttgatgta
    20821 cgacgggcat gtcgtatatc tgaggtgtaa gtcctcggtg ggcacctgct accggtgaac
    20881 ccaatagcga ttcccaagtc tgacgcagga aataagaatc gtcagaggaa gggatagcga
    20941 aatcgtggct ctacgaacag gaacatgata ataaggccta tatagcggat aaggtatctg
    21001 gctggggcga ccactatcaa ttatagtaaa atgaaataag agtaggacga atcgatcagg
    21061 gcagccaaat cgatttctaa caatgtttta gaagaagaga tgtactattc tagtttcaat
    21121 ctactatagt agctcagaag tcggtactta aacgtgctat atcaaaacca gtccttgaaa
    21181 aacgtggact ggtttcgtgt ttggattatt accttgaacg acatgcgtta acagttagtt
    21241 gaaccgccgt atgccgaacg gcacgtacgg tggtgtgaga ggggctagag attatcccct
    21301 actcgatttc gaaatctagt ggaatgaatc tggaatagtc catcgagctt tctaatactc
    21361 ttcgaaaatc tcttcaaacc acgtcaacgt cgccttgccg tgcgtatggt tactgacttc
    21421 gtcagttcta tccacaacct caaaacagtg ttttgagctg actacgtcag ttccatctac
    21481 aacctcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgct ctttggtttt
    21541 cattgagtat aacacattgt tagaagttgg tttaaattcc ctaatcagtt tgttcacatt
    21601 taccttcgat atattatatc ccatagttaa ggttggtcat acagatgatt atagtcatgg
    21661 agccgtaaaa cttagtgttt ctttagttga caaagatgcc atgaaaaaa
//
LOCUS       DAVKZB010000024        21001 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKZB010000024 DAVKZB010000000
VERSION     DAVKZB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.024"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            418..801
                     /locus_tag="VXA80_000814"
     CDS             418..801
                     /locus_tag="VXA80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV0353343.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVFYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            853..1542
                     /locus_tag="VXA80_000815"
     CDS             853..1542
                     /locus_tag="VXA80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353344.1"
                     /translation="MKKYQLLFKTSAILSYLFFVFGLFQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1584..1817
                     /locus_tag="VXA80_000816"
     CDS             1584..1817
                     /locus_tag="VXA80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353345.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1968..2579
                     /locus_tag="VXA80_000817"
     CDS             1968..2579
                     /locus_tag="VXA80_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353346.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSF
                     LVTSLSVAYARKFEMISFGMLSKKELLLFIAIFLLSVLETLVYIHFFAISSGAGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLTYL"
     gene            2741..3535
                     /locus_tag="VXA80_000818"
     CDS             2741..3535
                     /locus_tag="VXA80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV0353347.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGRYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQKTAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3532..4167
                     /gene="trmB"
                     /locus_tag="VXA80_000819"
     CDS             3532..4167
                     /gene="trmB"
                     /locus_tag="VXA80_000819"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV0353348.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4293..4772
                     /gene="rimP"
                     /locus_tag="VXA80_000820"
     CDS             4293..4772
                     /gene="rimP"
                     /locus_tag="VXA80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV0353349.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4816..5952
                     /gene="nusA"
                     /locus_tag="VXA80_000821"
     CDS             4816..5952
                     /gene="nusA"
                     /locus_tag="VXA80_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV0353350.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5974..6267
                     /locus_tag="VXA80_000822"
     CDS             5974..6267
                     /locus_tag="VXA80_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV0353351.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6260..6559
                     /locus_tag="VXA80_000823"
     CDS             6260..6559
                     /locus_tag="VXA80_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV0353352.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6576..9368
                     /gene="infB"
                     /locus_tag="VXA80_000824"
     CDS             6576..9368
                     /gene="infB"
                     /locus_tag="VXA80_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV0353353.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9619..9969
                     /gene="rbfA"
                     /locus_tag="VXA80_000825"
     CDS             9619..9969
                     /gene="rbfA"
                     /locus_tag="VXA80_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV0353354.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKVQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10248..11031
                     /locus_tag="VXA80_000826"
                     /pseudo
     CDS             10248..11031
                     /locus_tag="VXA80_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11033..11215
                     /locus_tag="VXA80_000827"
     CDS             11033..11215
                     /locus_tag="VXA80_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353355.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            11212..11352
                     /locus_tag="VXA80_000828"
     CDS             11212..11352
                     /locus_tag="VXA80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353356.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(11560..11634)
                     /locus_tag="VXA80_000829"
                     /pseudo
     CDS             complement(11560..11634)
                     /locus_tag="VXA80_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11665..11898
                     /locus_tag="VXA80_000830"
     CDS             11665..11898
                     /locus_tag="VXA80_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV0353357.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11898..13232
                     /locus_tag="VXA80_000831"
     CDS             11898..13232
                     /locus_tag="VXA80_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV0353358.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKDVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKRLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13242..13496
                     /locus_tag="VXA80_000832"
     CDS             13242..13496
                     /locus_tag="VXA80_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV0353359.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13592..13987
                     /locus_tag="VXA80_000833"
     CDS             13592..13987
                     /locus_tag="VXA80_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV0353360.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQDQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            14267..14827
                     /locus_tag="VXA80_000834"
     CDS             14267..14827
                     /locus_tag="VXA80_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353361.1"
                     /translation="MTRAELPDKIETERLVLRVRTVADAEDIFDYASRPEVSYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKELNLHKIELSCFGYNFQSQRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLRSEWEGI"
     gene            14827..15405
                     /locus_tag="VXA80_000835"
     CDS             14827..15405
                     /locus_tag="VXA80_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0353362.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLLYLKQIQDLLDDYHQEI
                     LFVELETSLEERIRRNRTENRLKHKPLKQHIEVSEREILDTAETLQLNSQHQPNELHH
                     YLKINNTNLSAEEVAKQIQDKMNTIEKGHTYI"
     gene            15650..16891
                     /locus_tag="VXA80_000836"
     CDS             15650..16891
                     /locus_tag="VXA80_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353363.1"
                     /translation="MAKKKNRKKELKRQQKLDIKVISEEEAAWTEIMAKNPTFVERRT
                     IQNFDELHTAVMQYADEYGKYPDVQLINYQLKNRTFDWKQDHAIFREAMHTPNTQKRN
                     KLLKQVLKINPDYFAADFHLFLSEVEDFDLSTFKKVLDFELLVLEKWKMNGYNSWNYF
                     EARPILSALMFLIEYYMMENFNSKAIELIDLYLSKRPERFPPNFVFCMLSLYHITGQE
                     LKVERFYREELNKGKRDDTILIHAIISAFSQGKIEEASQLFAKLVEINDEAVEFFIEE
                     DWQFKVIDIEDRECYCPNSVESLQASLYPLFDYLLENIILTKFLTNESKKFRRKPVFS
                     NHSGMIRNLSEVSDWHSFMNEEKMKGIRMDLVHIFVENGIRTSSDFKKWTEKEVLALK
                     GIGPVTVKKLKENGIKFKNEK"
     gene            16922..17923
                     /locus_tag="VXA80_000837"
     CDS             16922..17923
                     /locus_tag="VXA80_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEV0353364.1"
                     /translation="MNEISNFILYTTPNGHVQLDILLQDENLWLTQKGLAELFGVERS
                     VITKHLKNIYDSGELDKVSTCAKFAQVQQEGNRAVRRELEYYNLDAIISVGYRVNSSK
                     ATQFRIWATKTLKEFIIKGFVLDDERLEQGQTVFGQDYFRELLERIRSIRSSERRIYQ
                     QITDIFAECAIDYDQNSDITKNFYAMVQNKFHYAITGSTAAEIIYQQADASREKMGLT
                     SWKNAPDGRVLKSDVTVAKNYLQEKEIRQLERTVTAYFDYIEGLVERRNTFTMRGLAD
                     SIDRFLSFNEYEILEGKGSISKKQADSKAIQEYEKFNRTQKILSDFDKHILKIMRES"
     gene            18045..20696
                     /locus_tag="VXA80_000838"
     CDS             18045..20696
                     /locus_tag="VXA80_000838"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV0353365.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGREKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNDLAFEFAGMDRFEARKAVVAKLEEIGALV
                     KIEKLVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGEGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDVDSEDFKRYYPTSTLVTGYDIIPFWVSRMIFQGLEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLEQATANVEKVVNKEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWDEFADWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASNIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            20759..>21001
                     /locus_tag="VXA80_000839"
     CDS             20759..>21001
                     /locus_tag="VXA80_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV0353366.1"
                     /translation="MRSETRFLKNNGIYYTNNKLANIMIDNLEIDFDGNFTLLELAVG
                     EGHILCLILRRLLYHKQNCDSNKIKDFLENNIYAFDM"
BASE COUNT         6487 a         3860 c         4698 g         5956 t
ORIGIN      
        1 gtatagtatt tcttgtagtg tcagtagata atttttttag agacttaacc ccgttactgt
       61 tcattttaaa tattattggc ctgtcttgct tttcagtgct aacatacatt aacataaaag
      121 agatactctt aaatataagt aaatgatgat ttggttagtc ctagaaccaa tcaacttatg
      181 tttatcggct tggcagattt tatgtttgtc atttgtttgt atagaggtat ttcagaaaca
      241 gaattctatc aacaactaat cgcttatata ggtgtattct ctgcttgttt atcacgcttc
      301 tgctcatgtg gggcttgaag tattataaaa agtagctttc tatccttttc tgacaggata
      361 tttgagtaga aggctctatt aaattgttag tgaaataaat gaatggaggt atttaatatg
      421 aaatacagat tattttttgt tattttcttg agtagtgtgt tggatattct tttagggaca
      481 tttttacaaa tctctatcgt atctattggg tggcttgttt tttacagtgg tttgtttgaa
      541 gcaggagttt tccttcttgc taataaaggg gtggcggtaa aaatcaagga agtagatatt
      601 cgaaatcgct ttaaatttat ttttggaaaa accttatggt ttcaaattct tttgctcatc
      661 tttttgataa tcaaacttta tcttggtttg gatgcgaggt tgattttatt ctatggacat
      721 attttcattg tctttaatgc cttaatgtat ctattatcta gtagtcaggt tagccttaaa
      781 aaaaacaaac tgtcttctta atcttacagc agtagagcct gacaaggtga ggtcatagta
      841 gggaggattt ctatgaaaaa gtatcagctt ctattcaaaa caagtgcaat tttatcctac
      901 ctatttttcg tatttggtct ttttcagctg acgcttatcg tccaaaacta ttggcaattt
      961 tcttctcaga taggcaattt attctggatt caaaatatct tgagtttact ttttattgga
     1021 gtcatgattg tggttcttgt taagacaggc catggttatc tcttccgcat tccaagaaaa
     1081 aaatggcttt ggtattcgat tttgacagta ttagtggtag tgttccagat ctcttttaac
     1141 gttcagacag ctaaacatgt tcagtcaact gcggaaggtt gggctgtatt gattggttat
     1201 agtgggacta actttgcaga gctaggtatt tatatagccc tgttctttct ggttccactg
     1261 atggaagaat tgatttatag aggactactg caacatgctt tctttaagca ttcgcgattt
     1321 ggtcttgatt tgcttcttcc ttctatttta tttgctctcc ctcatttttc aagcctgcct
     1381 agtctgttag atatcttcgt ctttgcaaca gttggaatca tctttgctgg tttgacccgc
     1441 tataccaaga gcatttatcc atcctatgcg gtgcatgtga tcaataatat tgtagcgacc
     1501 ttcccgtttt tgctcacttt tctacatagg gtcttggggt aaaaaaacaa aaggcttgct
     1561 tttcagccat agaggaggtc atcatgtata aacacttatt tttcctagat tccaaaactt
     1621 tagatcggtt gacaccctat attctagtct tggcttctga caccattgct tttaatgttt
     1681 ttgtgctaac ctttgtatct gcggtggttt ttaatttcct aaattccatg ctagctttaa
     1741 tggctatatt cataggggct ggctatgtgg tcggattttg gttactaata ctcaatgaaa
     1801 atcaaagagc aaactaggaa actagccgca ggctgtactt gagtacggca aggcgacgtt
     1861 gacgtggttt gaatttgatt ttcgaagagt ataaaatggt ttgttttgga aaggctagaa
     1921 ctaaaggatg acgcgtagga agtctgtttg gtgaggagga tagttttatg gagttttttg
     1981 ataaatttca tgccttatgc tttggatttt tagtactaat aatcgttatt acagttcctt
     2041 atacgattaa ccatggggat ttttttcaaa atgaatctgc attgattatt gtaagttttc
     2101 ttgtaacctc gctgagtgtt gcttatgcta gaaaatttga aatgatttct tttgggatgt
     2161 taagcaagaa agagcttttg cttttcattg caatctttct tctaagtgtg cttgagacgc
     2221 tggtttatat tcatttcttc gctatttctt ctggcgcagg agttcaacac ttggcggaag
     2281 tcagcagagg aatttctctg tctttgattt tgactacctc agtttttggc cccatccagg
     2341 aggaactcat tttcagagga cttcttcaag gtgcggtttt tgacaactct tggttagggc
     2401 ttgtgctaac ttcctctctc ttttctttca tgcatggacc ttctaatgta ccttcgttta
     2461 ttttttatct acttgggggc ttgttactgg gctttgctta taaaaagagc caaaatttgt
     2521 gggtttctac tctagtccac atgttttaca acagttggcc actcttaact tatttataaa
     2581 aatcatgaaa aggtaagtag gagattgtgc ttaccttttt ctttctataa tgatactcaa
     2641 gccaatccag aggcttttat ttgagaatcg ggtgtggagc ggttgacgaa taggccaaaa
     2701 actagtagaa tagtaaggaa actttatacg gaggaaagaa atggatttgg gtgataatga
     2761 gctaacactg actcccatac ctgggaaaag tggcaaggct tatatgggta gatatcctga
     2821 tgggaagcgt atctttgtaa aaatgaacac ctctccaatc ctacctggtc tagctagaga
     2881 acaaattgct ccacaattat tatggagtcg tcgtttggca gatgggcgtg atatgtgtgc
     2941 tcaagaatgg ttgacaggaa agatattgac cccctatgat atgaatcgta agcaaatcgt
     3001 caatatttta acccgtcttc atcgctcacg tccgttgatg acacaattga gtcgtttggg
     3061 ctatgccatg gaaacacctg tagatttact acagtcttgg caaaaaacgg ctccagatgc
     3121 tttgcgtaaa aatcatttta tcagtgaagt gatggctgat ttacgtcaga ctattccagg
     3181 atttagagag gaccatgcga ccattgtcca tggagatgta cgacatagta attggattga
     3241 gacagatagt ggcttgattt atttagtaga ttgggattcg gttcgcttga ctgatcgcat
     3301 gtttgatgtg gcccatatgc tctgccatta tatttcagaa catcagtgga aggaatggtt
     3361 gacctactac ggttacaagt acaatcaaac ggtattaagt aaattgtatt ggtatggtca
     3421 attgtcttat ttgagtcaga tttccaagta ttatatgaac caagatttag aaaatgtcaa
     3481 tcgggagatt catggtttgc gtcatttccg agacaagtat ggaaagagaa gatgagagtt
     3541 agaaatcgta aaggggcaac agaattacta gaggcaaatc cccagtatgt ggtcctcaat
     3601 cccttggaag ccaaggcaaa atggcgggac ttgtttggca atgataatcc cattcatgtg
     3661 gaagttggaa gtggaaaggg tgcctttgtt tcaggtatgg ccaagcaaaa ccctgacatc
     3721 aactatatcg ggattgatat tcaaaagtct gttttgagct acgctttgga caaggtgctt
     3781 gaagctggag tgtctaacat caagctcttg tgggtagatg gttctgactt gactgactat
     3841 tttgaagacg gtgagattga tcgcttgtat ctgaactttt cagatccatg gccgaaaaaa
     3901 cgccatgaaa agcgtcgttt gacctacaag acctttttgg ataccttcaa acgtatcttg
     3961 cctgaaaatg gagaaattca tttcaagacg gataaccgtg gcttgtttga gtacagttta
     4021 gtgagctttt ctcaatatgg catgaaactc aatggtgtct ggctagattt gcatgccagt
     4081 gattttgaag gcaatgtcat gacagaatac gagcaaaaat tctcaaacaa ggggcaagtt
     4141 atctaccgag ttgaggcaga attttaagag atagcctaaa attaggctgt acaagtgctt
     4201 ttgctttaca taagttggca aacgtgctat actgatagta agaatatgaa aagtgaggcg
     4261 gggaaatatc ttcgcctctt gcttatgagg aggtggacgc aatcgcaaca atcgtagaat
     4321 tagtcagaga agttgtagaa cctgtcatag aagctccttt tgaactcgtg gatatcgagt
     4381 atggaaagat tggcagtgac atgattctca gtatttttgt agataaaccc gaaggaatta
     4441 ccttgaacga cacggcagac ttaacagaaa tgatcagtcc tgtcctagac accatcaagc
     4501 cagatccctt cccagaacaa tatttcctag aaattaccag tccaggcttg gaacgtcctt
     4561 tgaaaaccaa ggatgccgtc gctggagcgg ttggaaaata catccatgtc gggctctacc
     4621 aagccatcga taagcaaaag gtctttgaag gaaccttgtt ggccttcgaa gaggacgagt
     4681 tgactatgga atatatggac aagacgcgta agaaaaccgt ccaaattcca tacagtttag
     4741 tatcaaaagc acgtttagca gttaaattat agaaaaagaa aggatagctt ttgaggattc
     4801 aaaagtgaag aaaacatgag taaagaaatg ctagaggcct tccgcatttt ggaagaagac
     4861 aagggaatca aaaaagaaga tatcatcgac gcagtagtag agtcgcttcg ttccgcttat
     4921 cgcagacgct atggtcagtc agacagcgta gctattgact tcaacgaaaa aacaggtgac
     4981 tttacagttt atactgtccg tgaagttgtt gatgaagtat ttgatagccg tttggaaatc
     5041 agcttgaaag atgctcttgc cattaattca gcttatgaac ttggagacaa aatcaagttt
     5101 gaagaagcac cagctgagtt tggtcgtgta gcagcccaat ctgccaaaca aaccatcatg
     5161 gaaaaaatgc gcaagcaaac acgtgccatc acttacaata cttacaaaga acatgagcaa
     5221 gaaatcatgt ctggtacagt agaacgcttt gacaaccgct ttatctatgt caaccttggt
     5281 agtatcgaag cccaattgtc aaaacaagac caaattcctg gagaagtttt tgcttctcat
     5341 gatcgtatcg aagtttatgt ttacaaggtt gaagacaacc ctcgtggtgt gaacgtcttt
     5401 gttagccgta gccatccaga aatgatcaaa cgtttgatgg agcaagaaat tccagaagtt
     5461 tatgatggaa ctgttgaaat catgagcgtg gctcgtgaag caggtgaccg tacgaaggtt
     5521 gctgttcgta gccacaatcc aaacgtggat gctatcggta caatcgttgg acgtggtggt
     5581 gctaatatca agaagattac tagcaaattc cacccagctc gttacgatgc taaaaatgac
     5641 cgcatggtac caatcgaaga aaatatcgat gttatcgagt gggtagcaga tccagctgaa
     5701 tttatctaca atgccatcgc tcctgctgag gttgaccaag ttatctttga tgaaaacgac
     5761 agcaaacgtg ccttggtggt tgttccagat aacaagcttt ctcttgccat tggtcgtcgt
     5821 ggacaaaacg tgcgcttggc ggctcacttg actggttacc gtatcgatat caagtctgct
     5881 agcgaatttg aagccatgga agacgctgct tcagtagagt tggaagtaga aaacgatact
     5941 gtagaagaat aaaagctgct agaggaggga aagatgaaaa caagaaaaat ccctttgcgc
     6001 aagtctgttg tgtctaacga agtgattgat aagcgtgatt tgctccgcat tgtcaagaac
     6061 aaggaaggac aagtctttat tgatcctacg ggcaaggcca atggccgcgg cgcttatatc
     6121 aaactagaca atgcagaagc cctagaggcg aaaaagaaga aggtctttaa ccgcagcttt
     6181 agcatggaag tggaagaaag cttttatgac gagttgatcg cttatgtgga tcacaaagtg
     6241 aaaagaagag agttgggact tgaataagca aaagataagt aatctcttgg ggcttgctca
     6301 gcgagcaggg cgcatcatat cgggtgaaga attggtggtc aaggccattc aagacggcaa
     6361 ggccaagttg gtctttctag ctcatgatgc tggacccaat ctgaccaaga agattcaaga
     6421 taaaagtcat tattatcaag tagaaattgt aaccgtgttt tcaacactgg aattaagcat
     6481 agcagtcggg aaatcgagaa aggttttggc tgtaacagat gctggattta caaagaaaat
     6541 gaggtctctt atggaataga agaggaggac atgatttgtc taagaaaaga ttgtacgaaa
     6601 tcgcaaaaga acttggaaaa gaaagtaaag aagttgtagc gcgtgcaaaa gagttaggct
     6661 tggatgtgaa aagccactca tcaagtgtgg aagaagctgt cgctgcaaaa attgctgcca
     6721 gctttaagcc tgcagctgct ccgaaagtag aagcaaaacc tgcagcacca aaagtaagtg
     6781 cagaaaagaa agccgaaaaa tctgagccag ctaaaccagc tgtagctaag gaagaggcaa
     6841 aaccggctga gccagttgct ccgaaaacag aaaaagtagc agtgaaaccg caaagtcgta
     6901 atttcaaggc tgagcgtgaa gcccgtgcta aagagcaggc agaacgacgc aagcaaaata
     6961 agggcaataa ccgtgaccaa caacaaaacg gaaaccgtca gaaaaacgac ggccgtaatg
     7021 gtggaaaaca aggtcaaagc aaccgcgaca atcgtcgctt taatgaccaa gctaagaagc
     7081 agcaaggtca gcaaaaacgt agaaatgagc gccgtcagca agaggataaa cgttcaaatc
     7141 aagcggctcc acgtattgac tttaaagccc gtgcagcagc cctaaaagca gagcaaaatg
     7201 cagagtacgc tcgttcaagt gaggaacgct tcaagcagta tcaggctgct aaagaagcct
     7261 tggctcaagc taacaaacgc aaggaaccag aggaaatctt tgaagaagcg gctaagttag
     7321 ctgaacaagc acagcaagtt caagcagtgg ttgaagtcgt ccctgagaaa aaagaacctg
     7381 cagtggatac acgtcgtaaa aaacaagctc gaccagacaa aaatcgtgac gattatgatc
     7441 atgaagaaga tggtcctaga aaacaacaaa agaatcgaag tagtcaaaat caagtgagaa
     7501 atcaaaagaa tagtaactgg aataacaaca aaaagaacaa aaaaggcaat aacaagaaca
     7561 accgtaatca gactccaaaa cctgttacgg agcgtaaatt ccatgaattg ccaacagaat
     7621 ttgaatatac agatggtatg accgttgcgg aaatcgcaaa acgtatcaaa cgtgaaccag
     7681 ctgaaattgt taagaaactt ttcatgatgg gtgtcatggc cacacaaaac caatccttgg
     7741 atggggaaac aattgaactc ctcatggtgg attacggtat cgaagcaaaa caaaaggttg
     7801 aagtggataa tgctgacatc gaacgtttct ttgtcgaaga tggttatctc aatgaagatg
     7861 aattggttga gcgtccacca gttgttacta tcatgggaca cgttgaccac ggtaaaacaa
     7921 cccttttgga tactcttcgt aactcacgtg ttgcgacagg tgaagcaggt ggtattactc
     7981 agcatatcgg tgcctaccaa atcgtggaaa atggtaagaa gattaccttc cttgatacac
     8041 caggacacgc ggcctttaca tcaatgcgtg cgcgtggtgc ttctgttacc gatattacga
     8101 tcttggtcgt agcggcagat gacggggtta tgcctcagac tattgaagcc atcaaccact
     8161 caaaagcagc taacgttcca atcatcgtag ccattaacaa gattgataaa ccaggtgcta
     8221 acccagaacg cgttatcggt gaattggcag agcatggtgt gatgtcaacc gcttggggtg
     8281 gagattctga atttgttgaa atctcagcta aattcaacca aaatatcgaa gaattgttgg
     8341 aaacagtcct tcttgtagct gaaatccaag aactcaaggc agacccaaca gtgcgtgcaa
     8401 tcggtacggt tatcgaagcg cgcttggata aaggaaaagg tgcggtcgca acccttcttg
     8461 tacaacaagg taccttgaat gttcaagacc caatcgttgt cggaaatacc ttcggtcgtg
     8521 tccgtgctat gaccaacgac cttggtcgtc gtgttaaagt tgctggacca tcaacaccag
     8581 tctctatcac aggtttgaac gaagcaccga tggcgggtga ccactttgcc gtttacgagg
     8641 atgaaaaatc tgcgcgtgca gcaggtgaag agcgtgccaa acgtgccctc atgaaacaac
     8701 gtcaagctac ccaacgtgtt agccttgaaa acctctttga tacccttaaa gctggggaac
     8761 tcaaatctgt taatgttatc atcaaggctg atgtacaagg ttctgttgaa gccctttctg
     8821 cctcacttca aaagattgac gtggaaggtg tcaaagtgac tatcgtccac tcagcggtcg
     8881 gtgctatcaa cgaatcagac gtgacccttg ccgaagcttc aaatgccttt atcgttggtt
     8941 tcaacgtacg ccctacacca caagctcgtc aacaagcaga agctgacgat gtggaaatcc
     9001 gtcttcacag cattatctac aaggttatcg aagagatgga agaagctatg aaagggatgc
     9061 ttgatccaga atttgaagaa aaagttattg gtgaagcggt tatccgtgaa accttcaagg
     9121 tgtctaaagt gggaactatc ggtggattta tggttatcaa cggtaaggtt gcccgtgact
     9181 ctaaagtccg tgttatccgt gatggtgtcg ttatctatga tggtgaactc gcaagcttga
     9241 aacactataa agacgacgtt aaggaagtta caaacggccg tgaaggtgga ttgatgatcg
     9301 acggctacaa tgatattaag atggatgatg tgattgaggc gtatgtcatg gaagaaatca
     9361 agagataaga ttttttgctc ctttcttagg tggtgaggga cgcaagcaaa ccgatggttt
     9421 cattgcttat ttttgagcct agggtctcaa aaatcccctg tgatgggact gataaatcag
     9481 ttccatcact ttcaccacgg cgaaagaagc agatgacttc aaattgaact tcgtttcaat
     9541 ttaaactgaa aatcaagaag tttaaaatag ctaggtctgc tggcctagct tttggttcaa
     9601 agtagagaaa ggaatatcat ggcaaatcat ttccgtacag atcgtgtggg catggaaatc
     9661 aagcgtgaag tcaatgagat tttgcaaaag aaagtccgtg atccacgtgt ccaaggtgtg
     9721 accatcacag atgttcagat gctgggtgac ttgtctgttg ccaaggttta ttacaccatt
     9781 ttgagtaacc ttgcttcgga taaccaaaaa gtccaaatcg ggcttgaaaa agcaactggt
     9841 accatcaaac gtgaacttgg tcgcaatttg aaattgtaca aaatcccaga tttgaccttc
     9901 gtcaaagacg agtccatcga gtatggaaac aagattgacg agatgctacg caatctggat
     9961 aagaactaaa gaagaggggt tgcccctctt ttttggtgga ggaaaatagg ttgaatttga
    10021 aatggaaaaa tattctttta taatagattg aaactagaat agtacgcctc tacttctaaa
    10081 atattgttag aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcgtttca
    10141 atataaaaaa gggattctgt attttttaat gttatctaat tagaaaatgc tttttttgta
    10201 ggaaatataa tatgataagg tgcaaaaaag aaataaggag tttgtatatg gctgaacaag
    10261 acttagctat gcaagtattg caacaagtgg tgaaactacc tgttgttaag gttgatcgtt
    10321 cgaaattttt agtggataag ttttccaaag aattggatcc aaaagatatt cctaccttat
    10381 tggaacaagg tacaacgact cttctatctc aagaaatatt agatcgtgta gctaatgctt
    10441 gtattcggga caatgtatta ttagcgagtg ggacttctgt tttggcagga ttagctggag
    10501 ggcttgctat ggcaattacc attccagctt atgtggctca attttatggt ttctctctga
    10561 aattggccca agaattaggt tatatttatg gttatgagga tctttgggct tcacgtgagg
    10621 agttgagtga agatgctcaa aataccctct tgctttatct aggcgtaatg ttaggggtga
    10681 atggaaccgc tgctttgttt gctacgtgct ggtggtataa caattgccaa acaggtaatg
    10741 aaaacagtgc ctaataaagc tttaacaaag acgctttggt atcctatttt gaaaaaagtc
    10801 ttaaaaatat ttggtgtgaa tcttaccaag ggagggctgg ccaaaggaat agggaaattt
    10861 attcctatct tgggtggtat catttcaggt ggtttaacct ttgcaactat gaaaccaatg
    10921 ggggaaagct tgcagaaaga attatccaag ctagtcaact atagtgaagt tcaatatcaa
    10981 gaagatgttg aaacaatccg aaaagaggct gaaatcatca aaggagaata atatgaatcc
    11041 tatcaaagct tttgctaaaa tttatggtaa ttactttttg accgtgcaag gtgtaaaagt
    11101 gatgaaaacg ataaagaaag atgaccatgt cgttgttggt ctgggaaaac tctttattgc
    11161 agataagcta atggatacgg ctcggtggct cattaagcca gaggagagag aatgaaattt
    11221 ttctggacaa ttcttgctat tctttttatc aaaccgatta ttgggattgt gaaattcttt
    11281 tggatgatca tttcttttgc agttcaattg ctgttttaca agatattgga ttggttcttt
    11341 aaacttatct agatggtaat ccaagtcgca gagaactagc aggaactcca ctgctagttt
    11401 tttattctct ttccatatgg tataatataa gcagtaaaat cattttatac tcttcgaaaa
    11461 tctcttcaaa ccacgtcagc ttcaccttgc tgtatatatg ttactgactt cgtcagttct
    11521 atccacaacc tcaaaacggt gttttgagct gacttcgtca gttctatcca caacctcaaa
    11581 gcactgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    11641 ttagaacata caatggaggt cgtcatggac aatatcatcg atgtgtcaat tcctgttgca
    11701 gaagtggtgg acaagcatcc agaagtcttg gaaattctag tggagttggg ttttaaaccc
    11761 cttgccaatc ccttaatgcg caatacagtt ggtcgtaaag tatcacttaa acagggttct
    11821 aagctagcag gaactcctat ggacaagatt gtacgcacac tggaagcgaa tggctacgaa
    11881 gtgattggat tagactaatg gcagatgaac ggattcatgt cctacgggat attttattag
    11941 aattgcataa tggcgcctct cctgagtcgg ttcaggagcg ttttgatgcg acctttgcgg
    12001 gtgtctcggc cattgagatt tcccttatgg agcacgagct gatgaactca gactcaggtg
    12061 tcacctttga agatgttatg gaactctgtg atgtccatgc caatcttttt aaaaatgcta
    12121 tcaaagatgt cgaagtttca gatactgagc atccaggtca cccagttcgt gtcttcaaag
    12181 aagaaaatct ggctctccgt gcggccttga ttcgcattcg tagattgtta gatacctatg
    12241 agttgatgga agacgaggaa atgcttgcgg agatgcgcaa gggcttggtg cgtcagatgg
    12301 gacttgtggg tcaatttgac atccattacc aacgtaagga agaactcttc tttcctatca
    12361 tggagcgcta tggacacgat tcacctccca aagttatgtg gggagtggat gatcagatta
    12421 gggaactctt tcaaacagct ctaacgacag ccaagtcact accagaagtg tcaattagca
    12481 gtgtaaagga agcttttgaa gcttttgcga cagagtttga aagtatgatt ttcaaggaag
    12541 agtccatcct gctcatgatt ctccttgagt cttttactca ggatgactgg cttcagattg
    12601 cggaggagag tgatgcctat ggctatgcca tcatccgtcc gtcagagaaa tgggtgccag
    12661 aacgacagat ctttattgag gaaaagattg cagaggagcc tgtacagcta gatacggcag
    12721 aaggtcaagt tcaacaagtc atagatacgc cagaaggcca ttttaccatt acctttaccc
    12781 ctaaggaaaa ggaagctgtg ctggaccgcc atagtcaaca ggcttttggt aatggctatc
    12841 tttcagtcga gcaggccaat ctcatcctca atcatctccc tatggagatt acctttgtca
    12901 ataaagaaga tattttccag tattacaatg acaatacgcc agctgatgag atgattttca
    12961 aacggacgcc gtcccaagtc gggcgcaatg tcgaactctg tcatccgcct aagtatttgg
    13021 acaaggtcaa ggctatcatg aagagacttc gtgaggggac caaggacaag tatgaaatgt
    13081 ggttcaagtc tgagtcgcga ggtaagtttg tccacatcac ctatgctgca gtacacgatg
    13141 aagacggaga attccaagga gtgttggagt atgttcagga tatccagccc taccgtgaga
    13201 ttgatacgga ctattttcgt ggattagaat aaggagaaaa aatgagttac gaacaagaat
    13261 ttatgaagga atttgaagct tgggtcaata cccaaatcat gattaacgat atggcgcaca
    13321 aggaaagtca aaaagtttac gaagaagacc aggacgagcg tgccaaagat gccatgattc
    13381 gctacgagag tcgcttggat gcttatcagt tcttgcttgg taagtttgaa aacttcaaag
    13441 caggcaaggg attccatgat ttgccagaag gcttgtttgg tgagcgaaat tattaaacga
    13501 gaaagattct tgatttttca ctaaaatctt gatagaatgt ttatgttaaa tccttgtcag
    13561 agcagggatt ttttattgaa aggattttat catgtcaaag aaactcaatc gtaaaaaaca
    13621 attacgaaat ggcctccgtc gcgcaggtgc cttttcaagt actgtgacta aggttgtaga
    13681 tgagacaaaa aaagtcgtga agcgtgcaga acagtcagca agcgcagctg gtaaggctgt
    13741 ttctaaaaaa gttgaacaag cagtagaagc taccaaagag caagatcaaa aagtagctaa
    13801 ttctgtagaa gattttgcag caaatttggg tggacttcca cttgatcgtg ccaagacttt
    13861 ctatgatgaa ggaatcaagt ctgcttcaga tttcaaaaac tggactgaaa aagaactcct
    13921 tgccttgaaa ggaatcggcc cagctaccat caagaaattg aaagaaaatg gcatcaagtt
    13981 caagtaattt ttcttgagcc ttgcatttcc gaaaaaatct tgctacaata gagccattag
    14041 aggtgttttg aatcccacat tttacagaaa gtggcggcgc tgagaagtcc acaaatgtgt
    14101 caaaactggt tgctaatgga tgaaaaattg aaataaaagt gtctttttgc tttaaagacg
    14161 agagttgcgg gcaactgccc gaaattgggt ggtaccgcgg ataaacacat tcgtccctgt
    14221 catgtagatg gcaggggcga ttttttgtag aaagcgagga agaaagatga caagagctga
    14281 gttgccagat aaaatcgaaa cagaacgtct cgttttacga gtccgcacag tggcggatgc
    14341 tgaggatatc tttgattatg ctagtcgtcc agaagtttct tacccagcag gttttcctcc
    14401 agtcaagacc ttggaagatg agatttatta tctggagcac attcttccgg agcgtaatca
    14461 aaaggaaaat ctcccagcag gctacgggat tgtcgtcaaa ggaacggata agatcgttgg
    14521 ctctgtcgat ttcaaccatc gccatgaaga tgatgtgctg gaaatcggct ataccttaca
    14581 cccagactat tgggggcgag gttatgtgcc agaagctgcg cgtgccttga ttgatctagc
    14641 ttttaaagaa ttgaatctac ataaaattga attgtcttgc tttggatata actttcaaag
    14701 tcagcgagtc gcagagaagc ttggcttcac cctcgaagct cgtatccgag accgcaaaga
    14761 tgttcaagga aaccgctgtg acagtctgat atatggcttg ctgaggagtg agtgggaggg
    14821 gatttgatgc atcttttcat cattggtgct ccagcatcag gcaaaatgac tattggtcaa
    14881 gagttatctc gactgacaga tgctacacta ttttataacc atcaagctat tgattttgca
    14941 ctagaaatct atcaggacta cacagaggaa atgtgggaat ttgttcgtgg aattaccttt
    15001 tctttccttg gagcaagtgc aagaaatcag cgatcagtaa ttttaacaga cgtaattgat
    15061 ttttcaaatc agtaccagct gctgtatttg aagcaaattc aggatttgtt ggatgactat
    15121 catcaagaga ttctttttgt ggagttggaa acaagtcttg aagagcgcat acgtcgaaat
    15181 cgaacggaga atcgattaaa gcacaaaccc ttaaaacaac atattgaggt atctgaaaga
    15241 gaaattttag atacagctga aacacttcaa ttaaattccc aacatcaacc gaatgagttg
    15301 caccactacc ttaaaataaa taatacgaat ctgtctgcag aagaagttgc taagcagatt
    15361 caagataaaa tgaatacaat agagaaagga cacacatata tctaaacaac ttcatgaaga
    15421 cgttctcaag actcaagaag ttcaaaagtc tcaatctttt tcgttcgtga ttccgaccac
    15481 caaacgtaac tagaaaactc taccttaatc acacctgggg tacaaccttc aaagcaaatg
    15541 aatcacaaag aatctgagat ttaccattaa agcctgtatc cgaggccgta agaatatcca
    15601 atacagttga gataggacct attgaagaat gagttagagg agaaaataga tggctaaaaa
    15661 gaaaaataga aaaaaagaat taaaacgcca gcaaaagttg gatatcaaag ttatttctga
    15721 agaagaggct gcttggacag aaatcatggc caaaaatcca acttttgtag aacgtcgtac
    15781 tattcaaaat ttcgatgaac tacatacagc agtgatgcaa tatgctgatg aatacggaaa
    15841 gtatccagat gttcagctaa ttaattacca actgaaaaat aggacttttg attggaaaca
    15901 ggatcatgcc atatttagag aagctatgca tactccgaat acacagaagc gaaataaact
    15961 gttaaagcaa gttttaaaga tcaatccaga ttactttgct gctgattttc acttattctt
    16021 atcagaggtt gaagactttg atttgtcaac ctttaaaaaa gtattggatt ttgaacttct
    16081 agttttagaa aaatggaaaa tgaacggcta caatagttgg aattattttg aagctcgccc
    16141 tatcctgtct gctctgatgt ttctgattga gtactatatg atggagaact tcaattctaa
    16201 ggctatagaa ttgattgacc tatatttgag taagcgacca gaacggtttc ctcctaattt
    16261 tgtcttctgt atgctttcct tgtatcatat tactggtcag gaactaaagg ttgaacgttt
    16321 ctatcgtgaa gaattaaata aagggaaacg ggatgatacc attcttattc atgccatcat
    16381 ctctgctttt tctcaaggaa aaattgaaga agcaagtcag ctatttgcaa aattagtgga
    16441 aatcaatgat gaagctgttg aattctttat agaggaagat tggcaattca aagttataga
    16501 tatagaagac cgagaatgct attgtccaaa ttcagttgaa tcgctacaag ctagtttgta
    16561 tcctctgttt gattatttac tagaaaatat tattttaacg aaatttttaa caaacgaatc
    16621 caaaaaattc cgccgtaaac cagtattttc gaatcattct ggtatgataa gaaatttatc
    16681 cgaggtatcc gattggcatt cctttatgaa tgaggaaaaa atgaagggaa ttcggatgga
    16741 tcttgttcac atctttgttg aaaatgggat tcgtacatct tcagatttca aaaaatggac
    16801 tgaaaaagaa gttctcgctt tgaaagggat tggtcctgta accgttaaaa agctgaaaga
    16861 aaacggtata aagtttaaga acgagaagta attgtttatg gaagggggga ggtgaattgt
    16921 cttgaatgaa atttctaatt tcatcctata taccacaccc aatggccatg ttcagctaga
    16981 tatcctttta caagatgaaa atctttggtt gactcaaaaa ggtcttgcag agctctttgg
    17041 agttgaacga agtgtcatta caaagcactt gaaaaacatt tatgatagtg gtgagttaga
    17101 taaggtgtca acttgtgcaa aatttgcaca ggttcaacaa gagggaaata gagctgtccg
    17161 gcgtgaactt gaatattaca atctggatgc tatcatttca gttggttatc gtgtaaattc
    17221 cagcaaggct actcaattta gaatttgggc aaccaagacc ttgaaagagt ttatcatcaa
    17281 aggttttgtt ttagatgatg aacgcttgga acaaggacag acagtttttg gacaagatta
    17341 tttccgtgag cttttagaac ggattcgctc tattcgttct agcgagcgcc gaatctatca
    17401 acaaattacg gatatctttg ctgaatgtgc catagattat gatcaaaact cagatattac
    17461 taaaaatttc tatgctatgg tgcaaaataa atttcattat gcaattactg gaagtactgc
    17521 tgcagaaatt atctatcaac aagccgatgc tagtagagaa aaaatgggat taacttcttg
    17581 gaagaatgca cctgatggac gagtgctcaa gtcagatgta acggtagcca aaaattattt
    17641 gcaagaaaaa gaaattcgac aactagagcg aactgttaca gcctattttg attatatcga
    17701 gggtttggtt gagcgccgta atacttttac aatgcgagga ttggcagata gtattgatcg
    17761 ctttctatct tttaatgagt acgaaatttt agaaggaaaa ggaagtattt ccaagaaaca
    17821 agcagactca aaagccatcc aggagtatga aaaatttaac cgaactcaaa aaattctatc
    17881 ggactttgat aaacatattc tgaagataat gagagaaagt taatgtaaaa ttttcaccat
    17941 ttctttgctt atagaagata ctgaaaaaat cagatgtgct gaatcaaggc ctatcttcta
    18001 catactaatt ttatttaaag atattaacta gaaaggacaa acacatgtct aaagaacttt
    18061 cacctaaata caatccagcc gaggttgagg ctggtcgtta ccaaaaatgg cttgacgctg
    18121 atgttttcaa gccttcagga gatcaaaagg ctaagcctta ttcgatcgtg attccaccac
    18181 caaacgttac tgggaaactt caccttggtc acgcttggga tacgactttg caagatatca
    18241 tcatccgtca aaaacgtatg caaggttttg acacgctttg gcttccaggt atggaccacg
    18301 cggggattgc gactcaggct aaggttgagg agcgcttgcg tggagagggc attacacgtt
    18361 atgacctcgg tcgtgagaaa ttcctcgata aagtttggga atggaaagat gagtatgcca
    18421 ctaccatcaa agaacaatgg ggcaagatgg ggctctctgt agactactct cgcgagcgtt
    18481 tcacccttga cgaaggtttg tcaaaagccg ttcgcaaggt ctttgtggac ctttacaaga
    18541 aaggctggat ctaccgtggt gagtttatca tcaactggga cccagcagct cgcacagccc
    18601 tttctgatat cgaggttatc cacaaggacg ttgaaggtgc cttctaccac atgaactaca
    18661 tgctagaaga cggctcacgc gcccttgaag tggcgacaac tcgtcctgag accatgtttg
    18721 gggatgtggc ggttgcggtt aatccagaag acccacgcta caaggacttg attggaaaaa
    18781 acgtcatcct tccaatcgct aataaattga tcccgatcgt tggagatgag cacgccgacc
    18841 ctgagtttgg tactggtgtc gtaaaaatca cccctgccca cgatccaaat gacttcttgg
    18901 ttggtcaacg tcacaacttg ccacaagtca acgtcatgaa cgatgacgga actatgaatg
    18961 acttggcttt cgaatttgca ggcatggacc gttttgaagc tcgtaaggca gtcgttgcca
    19021 agttggaaga aatcggtgct cttgtcaaaa tcgaaaaact tgttcacagt gttggccact
    19081 cagaacgtac aggtgtcgtg gttgagccac gcttgtctac tcaatggttc gtcaagatgg
    19141 accaattggc caaaaatgct attgccaacc aagacacaga tgacaaggta gaattctacc
    19201 cacctcgttt caacgatacc ttccttcaat ggatggaaaa tgttcacgac tgggtaatct
    19261 ctcgtcagct ctggtggggt caccaaatcc ctgcttggta caatgctgag ggtgaaatat
    19321 atgtcggcga agaagctcca gaaggtgaag gttggactca ggacgaagat gtcttggata
    19381 cgtggttcag ttcggccctt tggccattct ctaccatggg ctggccggat gtcgactcag
    19441 aagacttcaa acgttattat ccaacatcaa ctttggtgac tggttatgat attatcccgt
    19501 tctgggtatc tcgaatgatt ttccaaggtt tggaatttac tggcaaatcg ccattcaaaa
    19561 atgcattgat tcatggtctg attcgtgacg aggaaggccg taagatgtct aaatcactgg
    19621 gcaatgggat tgatccgatg gatgttattg ataagtacgg aacagatagc ctgcgttggt
    19681 tcctttcaaa cggttctgca ccaggtcaag acgtgcgctt ctcttatgag aaaatggatg
    19741 cttcatggaa cttcattaac aagatttgga acatctctcg ctacatcctc atgaacaatg
    19801 aaggtttgac ccttgagcaa gcaactgcca atgtggaaaa agttgttaac aaggaagctg
    19861 gaaatgtcac agaccgctgg attctccaca acctcaatga aaccatcgga aaagctactg
    19921 ctaactttga caagtttgaa tttggtgtgg ctggccacat cctctacaac tttatctggg
    19981 atgaatttgc ggactggtac gttgaattga ctaaggaagt cctttatagc gacaacgaag
    20041 aagagaaagt catcacacgc tctgttctcc tttacactct ggacaagatc cttcgtctcc
    20101 ttcacccaat catgccattc gtgacagagg aaatctttgg acaaatctca gaaggttcta
    20161 tcgtgacagc agaataccca actgtcaacc cagcctttga agaccttgcg gctcacactg
    20221 gtgtcgaaag tctcaaagac ttgatccgtg ccgttcgtaa tgcgcgtgcg gaagtaaacg
    20281 tagcaccaag caaacctatc accatccttg ttaagacaag cgatagcgac ttggaagcct
    20341 tctttaacag caatgtcaac tacatcaaac gcttcacaaa tccagaacac ttggaaattg
    20401 catcaaatat ccctgcacct gaactcgcta tgtcaagtgt catcacagga gcagaaatct
    20461 acttgccact cgctgacctc ctcaatgtcg aagaagagct ggctcgtctc gacaaggaac
    20521 tggctaaatg gcaaaaagaa ctggacatgg tcggtaagaa gctctctaac gaacgcttcg
    20581 tagccaatgc caaaccagaa gtcgtccaaa aagaacgcga caaacaagcc gactaccaag
    20641 ctaaatacga cgcgacagtc gcacgtattg atgagatgaa gaaactagtg aaataaacat
    20701 agaaacctcg gcaatttgct ggggtttttg gtataataaa tcttgatgga tgaaaaatat
    20761 gagaagtgaa acaagatttt taaaaaacaa tggaatttat tatacaaata ataagcttgc
    20821 aaatatcatg atcgataatc tagagattga ttttgatggt aattttacat tactagagct
    20881 tgctgtcggt gaaggacata tattatgtct tattttgaga aggttactat accacaagca
    20941 aaattgtgat tcaaataaaa ttaaagattt tcttgaaaat aacatatatg cctttgatat
    21001 g
//
LOCUS       DAVKZB010000025        20240 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKZB010000025 DAVKZB010000000
VERSION     DAVKZB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.025"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(21..254)
                     /locus_tag="VXA80_000840"
     CDS             complement(21..254)
                     /locus_tag="VXA80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEV0353367.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCN"
     gene            complement(468..2099)
                     /gene="rlmD"
                     /locus_tag="VXA80_000841"
     CDS             complement(468..2099)
                     /gene="rlmD"
                     /locus_tag="VXA80_000841"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0353368.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2173..3147)
                     /locus_tag="VXA80_000842"
     CDS             complement(2173..3147)
                     /locus_tag="VXA80_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV0353369.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVVAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3221..3835)
                     /gene="pmp23"
                     /locus_tag="VXA80_000843"
     CDS             complement(3221..3835)
                     /gene="pmp23"
                     /locus_tag="VXA80_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV0353370.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3835..4809)
                     /locus_tag="VXA80_000844"
     CDS             complement(3835..4809)
                     /locus_tag="VXA80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV0353371.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4817..6073)
                     /locus_tag="VXA80_000845"
     CDS             complement(4817..6073)
                     /locus_tag="VXA80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV0353372.1"
                     /translation="MIFDKHDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAVAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6135..6566)
                     /locus_tag="VXA80_000846"
     CDS             complement(6135..6566)
                     /locus_tag="VXA80_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353373.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(6568..7170)
                     /locus_tag="VXA80_000847"
     CDS             complement(6568..7170)
                     /locus_tag="VXA80_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV0353374.1"
                     /translation="MMDRIRQELEKGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7154..7993)
                     /gene="prmC"
                     /locus_tag="VXA80_000848"
     CDS             complement(7154..7993)
                     /gene="prmC"
                     /locus_tag="VXA80_000848"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV0353375.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDATDYLKDSGKIYLEIGYKQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7993..9072)
                     /gene="prfA"
                     /locus_tag="VXA80_000849"
     CDS             complement(7993..9072)
                     /gene="prfA"
                     /locus_tag="VXA80_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV0353376.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9069..9659)
                     /locus_tag="VXA80_000850"
     CDS             complement(9069..9659)
                     /locus_tag="VXA80_000850"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV0353377.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GIGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKTYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9783..9965
                     /locus_tag="VXA80_000851"
     CDS             9783..9965
                     /locus_tag="VXA80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV0353378.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAIVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10103..11476
                     /gene="mnmE"
                     /locus_tag="VXA80_000852"
     CDS             10103..11476
                     /gene="mnmE"
                     /locus_tag="VXA80_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV0353379.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQETNRII
                     LLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDVAPDELITQLF
                     SQFCLGK"
     gene            complement(11523..12458)
                     /gene="dapA"
                     /locus_tag="VXA80_000853"
     CDS             complement(11523..12458)
                     /gene="dapA"
                     /locus_tag="VXA80_000853"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV0353380.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12514..13590)
                     /locus_tag="VXA80_000854"
     CDS             complement(12514..13590)
                     /locus_tag="VXA80_000854"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV0353381.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKT
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGAKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13932..14004)
                     /locus_tag="VXA80_000855"
     tRNA            complement(13932..14004)
                     /locus_tag="VXA80_000855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13969..13971),aa:Thr,seq:ggt)
     gene            complement(14104..14613)
                     /locus_tag="VXA80_000856"
     CDS             complement(14104..14613)
                     /locus_tag="VXA80_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV0353382.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14611..14707)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14736..15167
                     /locus_tag="VXA80_000857"
     CDS             14736..15167
                     /locus_tag="VXA80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV0353383.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15249..15626
                     /gene="mscL"
                     /locus_tag="VXA80_000858"
     CDS             15249..15626
                     /gene="mscL"
                     /locus_tag="VXA80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV0353384.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15668..16762)
                     /gene="hemH"
                     /locus_tag="VXA80_000859"
     CDS             complement(15668..16762)
                     /gene="hemH"
                     /locus_tag="VXA80_000859"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV0353385.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            16917..18137
                     /gene="pepT"
                     /locus_tag="VXA80_000860"
     CDS             16917..18137
                     /gene="pepT"
                     /locus_tag="VXA80_000860"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV0353386.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(18180..18809)
                     /locus_tag="VXA80_000861"
     CDS             complement(18180..18809)
                     /locus_tag="VXA80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353387.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            18774..18995
                     /locus_tag="VXA80_000862"
     CDS             18774..18995
                     /locus_tag="VXA80_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353388.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19072..19614)
                     /locus_tag="VXA80_000863"
                     /pseudo
     CDS             complement(19072..19614)
                     /locus_tag="VXA80_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19715..20236)
                     /locus_tag="VXA80_000864"
                     /pseudo
     CDS             complement(19715..20236)
                     /locus_tag="VXA80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5849 a         4255 c         3803 g         6333 t
ORIGIN      
        1 tctttaaatt cattggtatg ctaattacaa tatccattat taaaatggat actataagca
       61 gcacaccaac tatttatttt ttgagtttta aactttggta gaagtcttga actccttata
      121 tttacataac tatttgcctc tataactatt atgacacgaa accaacaagc aagttcaagc
      181 acctccatat ctttattcaa atgaggacta agatattcac aaactttatc ataaacctct
      241 ttatcttttt tcataaaagg ggcatacaac ttagcaaatt tatcctaaaa ttttttgttg
      301 cctttatcaa tcatttcaac gctcctttaa atataaaaat gatcctatca atctttatat
      361 tgagcctatt ttctaattga taccttgcaa gaaaggaata atagctgttc ctataccaag
      421 taggaatgta ataaatgcag aaagtcatat actgtcattc ttttctatta aatcattttg
      481 aagtgtctca aagcatccat gatggcttct tctttttcag gtgtacactg tggaataatt
      541 tgtttatcct ttttagactt gttgtaatgt tctcgtaatt ctattccaca tttctttttt
      601 atctgtgcaa tatataatgt cgaaactttt aattcaaatt tattccaaac atattctttg
      661 atttgagcat atgttgcttt gctctccgca cttgtcaaat ccatctcatc cagctcaatt
      721 tcaacactta tgtgcttatc gacatcgagt ttggacaaaa gtgctaccgt ctcgacgtga
      781 tgcgtttgag gaaatagatc caccggctgg actttcttca attcatatcc caactcttgg
      841 tatagtttaa tatcacgcgc catggttgcg acattgcagg agatataggc gatgcgatcg
      901 gctcctgttt gggcgcttgc tttgataaag ctttctgtca agcccttgcg tggaggatca
      961 accaagataa cggttggttg aataccttcc ttgagccatt tcttcatggc attttcagcc
     1021 gtgtcacaga catagtgggc attagtaatc ttgttcaaag aagcattctt ctggctattc
     1081 tctactgctt ctggaatcag ttcaacaccg tagacttctt tgacatgctt ggcgactgat
     1141 aaaccaatgg ttccaatacc agaataggca tcaataatca catcatcttt ttttaactct
     1201 gcaaagtcaa tggctgtttg atagagtttc tccgccattt cagtattgac ttggtaaaag
     1261 gctgggccag cgatttggaa gtcatttccc aacatctggt ccgtaatata gtcttgacca
     1321 taaagagtgc gccactcctt accaaaaatc gcattggtat tctggtcgtt gatattttgc
     1381 atgacagaca caatctctgg gaactgcttg ataacttgtt caatcaattg gtcaacacga
     1441 aaaacttttg gacgagttgt caccaaaacg accatgattt gtcctgaata gtgaccacga
     1501 cgcaccacaa gattccgaat caatccagac tgttcctttt cgtcataagg ttttaaatca
     1561 aaacgacgga gcaggtctcg aagagctact acgacttggt caatgacagg atcctggata
     1621 aagaaatctt caaggggcat gaggtcgtgc gaattcttac ggaaaaatcc tgtttccaag
     1681 acaccattca ctcgacgaac gggcacctgc gccttattgc gatacttgac tggatgttcc
     1741 ataccaagcg tttcagcaac ttctacatct gcaattccag caatcttgta gagactgtcc
     1801 ttgacttgct tggttttaaa cttgagctgt tctggataag aaaggtgtcc taaatccgcg
     1861 attcctgaac gcaggtaagc caaatctaga tcttgattac ggtgtggtga ctggacaagg
     1921 tatttttcaa cttttccaaa gccaatcttt ttattgacct tgaggacacg catgagaatt
     1981 ttttcactcg gtaaagcatt ctctacaaaa aagaccaaac catctacctt ggcaactcct
     2041 gccccttcat gggtcaaatc aacaatttca acttctacaa tatcattttt ctttaacatt
     2101 cttttctctt tctattggcc gacaaaaaag acggcaacgt tacggttacc atccattata
     2161 ccatgaaatt tcttaagaac caaaactctt gaagaagttg acaagggctt gccagaggga
     2221 gcttaagaag ttaccaccgt cctctagcgc cttatccgca tcaaagttaa ggttgatatt
     2281 tttaaaactg tcgccagctt gtgatacgat gctttgttta aggtcattta gggttttagt
     2341 gaaatctgca ttgctgagga tatcactctt tgagagattc aaggcaaaat tgatgatgat
     2401 attgatctgg tttcctgtta tgacctgatc aagtttgtaa ttttttaagg tatcttcaac
     2461 aatcttgcgg acatcttctt ctgtcagatt tcccttgctt tctttagctt tggcgagtcc
     2521 tgacttgata tcagctaggg caacgtttaa tttattagca tcatagcctg atttgtcctt
     2581 gttttcagca ttgatatctg acaaagcctt tagctcttct tgagccaaat ctttattggc
     2641 ttgcggcacc ttggctccat tagcttctag cgaatagtag atcccagcca aagcactctc
     2701 acctgtaact ggaatagggg ctactacagt gattttggca tgttccatac ccagcgttac
     2761 tgctgcgttt cggtacatat cctgagtcac cttagtgata ttttctggtg tttcaatctt
     2821 gacctcaagt ggcgatttgt cacctagctt ttgaatcttg gctgatgaat acaactgtaa
     2881 gctagagtca ttggccacat tcatgatttt agaataaaca tcaggtgtca tggtcttgag
     2941 ttctttggta tctgttgagg cattgtagcc cagtttttta agagtttgat ttttttggtc
     3001 ttcagatagg gaggaaccta ggacatattc aggttggaca taggtttcat cgataacttt
     3061 ttgaacatct gttgctgcat ggacgctatt catagctgtt actgcccaca agaccgcagc
     3121 actggtcaga aagagtttct ttctcatagg gaatttcctc ctttactttt ctagagtaat
     3181 atatctatct taaagaaaac ttataacaaa aacacctggt ctagccagat gttgaaaaga
     3241 gagtgaaaca tttgatgatg taaaggttaa gtcgtacctg tctagaataa taatagtttc
     3301 ctccatttac atagagttca gcaccgtgaa aaatggaaat ggggtgaata taactataag
     3361 tctttccagt cctattacca agcaaggggg caacagtctc acgagagtac tgtttggcta
     3421 gagccaaggt attttccttg ccattttggg cgataaaatc gatataggca ggtccaaaat
     3481 tataggcttg aacagctgtc cagatatcta cccccttctt ctgcgccaga tagagattgc
     3541 ctgtcagagt ttgaatgcct tgccgaatgc tagaggcatt atcattgatg gtgttggttg
     3601 aaccacttgc agactcacta gactgcataa catcgccttc ttttcctttt gtttcagtat
     3661 aaatcatagc aagcacaagc tcttcgtttg ctggggtgtc tttttcactc aagatttctc
     3721 gcaccatggg ttgataggtc atgacttgtt tgacatcttg atgaacgcgg taagctttat
     3781 agccagcaaa aaggaagact gctagtacaa gcactcttcg aattcgttta aacattattt
     3841 actttggata tcctcgatat ttttgattaa gatagagtag gttccatttt cgttttggat
     3901 aaactcaaca gactcggcgt cttgatagac gttattggga acgatgagct caattccatt
     3961 tgataaggag agtttttggt tttcaaattt ctttaattgg cgactggcat caatttcatc
     4021 aaattgaaca ggttctggta cggcttcttt gacttggtca ataaagctca aacgagccgt
     4081 cagattgttg tcaaaaaggt cattagccaa tttctcaggt gacaattcat tgctttcttc
     4141 taggttgttg aaaatcgctg atttgacctt ggattgaaat tgaaaatcat ctgtgttaaa
     4201 agattcagca attctctggg ctgttttttc cagttccttg atagattttt taggagaaat
     4261 cttaggagcg acagcaagaa gattatctga aaaatagttc aaaaaagtcc cgttgtactt
     4321 gattcgtttt tcaatcaggt gatacttgcg actctgaaga ttgaccacca aggcctcgtc
     4381 agcacccgtt ccaaatccag gcaggttatt ctgagtcagc ttgattggat tatcaacttc
     4441 tcctccgagg tgggtcaagg tctcccgcag ggcaattcgc aagaaagcga aatgttctac
     4501 accttcttta gaaaattgaa caaaaatcaa gtcattggtc ttgagatttt cagaaatgct
     4561 aaactcctct ttccagagat tagccagcgt tactgatgtc tccaacaaat cgtctgtaat
     4621 atgattgaag aagggatttt cttcttcgaa aatcccagtc ttggcttcat ctgaatacac
     4681 atgttcaatt tttttacgta ggtattcttc gatttttgga gtaatattga gaaacttatc
     4741 tgctaagaac agctcggtat catccggact gaactggtga ataatggctt tcttaatata
     4801 aatgtccata aaagttttat tcctcgtata atgggaaggc atctgtcaat tctttgactg
     4861 cacttctcac ttcttctaat acagcctcat tttctgaatt cttaagggtt ttaatgatga
     4921 gttcagccac tttgcgactt tcttcttcac caaatccacg tgcagtaata gctactgctc
     4981 cgatacgaat cccacttgtc ttgaatggtg acaaggtttc gtaagggatt gaatttttat
     5041 ttagggtaat attgacttca tccagcaagt tttgagcaac ttttccgttt tctacaacct
     5101 tagtcacatc aacaaggaag agatggtttt cagttccacc tgaaataata cggaaatcag
     5161 ggtcttgcaa gaagacatct gccatagcct tgctgttctt aattacattg gcagcatatt
     5221 ccttgaaggc tggatccaaa acttctttga aggaaactgc cttagccgcc acaacatgct
     5281 ctaaaggacc gccctgaata cctgggaaaa tagctgaatt gattttttta gcaagttctt
     5341 cgtcattagt caaaatcaaa ccaccacgag gtccacgaag ggttttgtgg gtcgttgttg
     5401 ttgtgatatg agcgtatgga actgggcttg gatgaaggcc agccgcaacc aagccagcga
     5461 tatgggccat gtccaccatg agcttcgcac cgacagcatc tgcgatttca cggaattttg
     5521 aaaaatcgat aatttgagaa taggctgaag caccagctac aatcagtttt ggttttactt
     5581 cttgggcttg tttcaagata gcatcaaagt ctaagagttc cgttttagga tcaacactat
     5641 aagaaacaaa gttgtaggtt tgaccagaga agctaacagg agccccatga gtcaaatgac
     5701 cacctgatgc caaatccatt cccataaccg tatcacctgg ctcaatcaag gacatgtaag
     5761 ccgcacagtt agcttggctt cctgaatgtg gttgaacatt ggcaaattta gcaccgaaaa
     5821 tttcttttgc gcgttcaata gcaagagtct ctacaacgtc tactacatca gttccaccat
     5881 aataacggcg tcctgggtaa ccctcggcat atttatttgt caagatagac ccttgagctg
     5941 ccataacagc cttggaaact acgttttccg aagcaattaa ctcgatatta ttttgttggc
     6001 gttcttcttc tttggcaata gcattccaga gatcagcatc atatgcttta aaatcatgtt
     6061 tgtcaaaaat cataggtctt ctcctttatt gtgtgactag tccattagtt tgattttaca
     6121 ataagaaaat caaactaaca gatgcgaata aaccgtttct gcattttatc acaagtatag
     6181 ccaacttttt cataaaatgc atgagcaccc agacgatgat tggcagaatt taagcggata
     6241 aacccataac cacatctttt tgcttcttct tccaaccctt gtagtaaact tttaccaata
     6301 ccttgacctt gcgcttgagg tgaaactgct aaagctaaga tattaaatcc tgctttggaa
     6361 tagagtgatt cgtaaacttc agcgtggaca tatccaagta agacatgatt agctgcatcc
     6421 tcatagccaa gtaggaaatg atgggaatcc tgagacagtc tagctagttg gctagccgtt
     6481 tcctctggac taaaagtata acccaaagcc tcttggttga tgtcacatat cgctttcaca
     6541 tctgtttctt gcaaatctct tagcatttca agcctcctca aaagaaatct ctggcaaccg
     6601 agcaagaata tcttctcgtt taattgcgcc ttggcgtaag attttcacct tgtctccaga
     6661 caaatccaca atagttgaat cctgtccagt tagaaaagca tcgtcttcca gacccagaac
     6721 ctcttggtca aaatccttca gaatttgttc aaaggttaca ccacttgcct gacctgagat
     6781 attggcagac ggcccaatca agggacctgt ctctcgaatt aaatccagtg tgatagggtg
     6841 actgggcatc cgaaatccaa tagttgcaag gtcagaattt acccaatagg gaactcggtc
     6901 attggcttcg agaataatgg tcaagggacc tggcaaaaag gtctctacaa gtttttgtag
     6961 ataagctggc tgattctttg aaaagtgcaa gatgtcctcg aaagaggcga tattgagatt
     7021 gagtgcctta tctctaggac gacgtttgag ttggtaaaca tggtcaactg ctttttcatc
     7081 taaggccttg gaaaaaagac cataaacagt ctctgtaggt agaacgacag ctccaccctt
     7141 ttccaactct tgtctaatcc tgtccatcat caaccacaac catcctattt tgaccaaatt
     7201 ggtctttgag tgttcgaact cttttttcag gaagatgttt tctaaaaagt tcgggaacac
     7261 attgaccttg cttgtatcca atttcaaggt aaatcttacc actatcttta agatagtctg
     7321 ttgcatcttc cgcaattcta cggtaaatag ctaggccatc ctcgtctgca aagagagcta
     7381 gatgaggctc cgaatacaag acattcaagc ctacctctga ctcatcttca cgagagatat
     7441 agggtggatt ggaaacaatt atatcatatt tttcagaaat ttctgtaaaa cagtcagatt
     7501 tttttaaaaa tatttgaaga ttttgatttt tagcattttc tctagctaaa tctaaggcct
     7561 cttgagaaat atctgctgct gtcactgacc aatctggtct attttttgct agagcgagag
     7621 caatagctcc actacctgtt ccaatatcca gaactgagag attcgtctca agattttcag
     7681 ttagaataag ctccactaac tcctctgttt ctggacgagg aatcaaaacc cgttcatcca
     7741 cctttaaatg cattccataa aaatctgcct gtccaatgat gtactgagct ggcttgtgag
     7801 ctgctagttg ctggtaaata tcttctacaa attgtttttc ttcctctgtt gtcacctcct
     7861 gctggagggc aaaaataaag tctgtaaaag atagattttt cagactacga tagacaaaag
     7921 agaggctttc cgcttcctct ccttgtctta tcaactcttc ttcaaaattt gaaaataatt
     7981 gagctaattt cattatttgt ttaattcttc tagtttttgt gtttggtcat aaagcaccaa
     8041 ggcatccaca acttcgtcca atttaccaga caaaatcgta tctagttttt ggagggtcaa
     8101 gccgatacgg tggtctgtga cacggttttg agggaagtta taagttcgga tccgttctga
     8161 acggtcacca gtaccgattg tcgacttacg ctcagcgtct tgttcatcct gagcaatctg
     8221 agcaaagtgg tcagcgacgc gtgcacggat aatcttcata gccttctcac ggttcttctg
     8281 ctgggtacgt tcttcctgca tctcaacctt gatattggtt ggcaagtgaa cgatacgaac
     8341 ggcagtcgca accttattga cgttctgtcc accagcacct gatgcgtggt aaatatcaac
     8401 acgaagatct tttggatcga tatcgtattc tacttcttcg acttcaggca tgacaagaac
     8461 tgtggcagtt gaggtatgaa cacgaccttg gctttctgtc acggggacac gctgcacacg
     8521 gtgggcacct gattcgtact taagcttaga gtatacagac tgacctgaaa ccatagcaac
     8581 cacttcttta aacccaccga caccattcat agaggcttcc atgacttcaa agcgccagcc
     8641 ttgggcttcc gcatactttt ggtacatagt tagcaaatct ccagcgaaaa gtgccgcttc
     8701 gtctccacca gctgctccac ggatttcaag gatgatattc ttgtcatcgt ttggatcctt
     8761 tggaaggagc aaaattttca gtttttcttc atattcttct ttttcagcct tggcatcttt
     8821 gagttcttgc ttggccattt cttccaagtc cgcatctccg cctgattcct taatcatctc
     8881 ttcggcatcg acgatatttt gaaggacttg tttatactca cggtaggcta ttacggtatc
     8941 acgattggaa gcttcttctt ttgaaagctc cataaaacgc ttggtgtctg aaacgacatc
     9001 agggtcactc agcaattctc ctagttcttc ataacggtct tctacagctt gtagttgatc
     9061 atagatgttc atttttcttc attctcctta ttgatttcag gcgcaaaata atgtttacgg
     9121 caaaccgaga tataggtttc attaccacca atctgaatct gctcaccatc atagaccggc
     9181 agtccatcct gtgttcgcaa aaccatagtc gcctttttct tacaatactg gcagatggtc
     9241 ttgatttcgt caatcttatc tgctaaaagc aagagatact tggaaccttc gaacagttca
     9301 ttgcgaaagt catttttcaa accaaaagcc atgacaggta tgtctaactc atcaacaaca
     9361 cgagctaggt cgtaaacatg gtgacgtttg agaaactgag cctcatcaac caatacacag
     9421 taagtttttt ctggtaggtc tcggatatag ccaaagatat ccgtcgtctc ctcaatcgca
     9481 agagcagggc gtttcatacc gattcgactc gacacatagc caataccgtc acgcgtatcc
     9541 agagccgagg tcataatcac aacacctttt ccttgctcct catagttata ggccaccttg
     9601 agaatctcaa tcgtcttacc agagttcatg gtcccataac gataatacaa ctgtgccatg
     9661 tttcttgctt cacgtccatt tctaaatttt tgctacattc tagtatatca taattttctg
     9721 aagctttaaa cggcaaaatg tggtaaaata gaagaaatca aaaactagtg gaggaagcta
     9781 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
     9841 ttgctaagga agtaacggaa gctattgtcc gtaatactgg agccccccaa tctgctgtcc
     9901 atgtcatcat caacgacatg ccagaaggaa cttacttccc acaaggggaa atgcgtacca
     9961 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtaa catccattga
    10021 agaaatagcc taaatttgtt acaatttgaa aggatacttg gaaaaatttc caagaaaaga
    10081 gctatttatt aaaggaaaca ttatgattac acgtgaattt gataccatcg ctgctatctc
    10141 tactccacta ggtgaagggg ctattggtat tgtccgcctg agcggaacag acagttttgc
    10201 tattgcgcaa aagattttca aaggaaaaga cttgagtcag gttgctagcc atacacttaa
    10261 ctacggtcac atcgttgacc ctctgactgg taaagtcatg gacgaggtca tgattggggc
    10321 tatgaaatct ccaaaaacct tcactcgtga agatattatc gagattaaca cccacggtgg
    10381 gattgcggtg actaatgaaa ttctccagct agctatccgt gaaggagctc gattggctga
    10441 acctggtgaa tttactaaac gtgccttcct aaatggacgt gttgacttga ctcaggctga
    10501 ggcagtaatg gatatcatcc gtgctaagac agataaagcc atgaacattg cggtcaaaca
    10561 actagatggt tctctttctg acctcattaa caatacccgt caagaaatcc tcaatacact
    10621 tgcccaagtc gaagttaata tcgactatcc tgagtatgat gatgttgagg aagcgactac
    10681 tgctgttgtc cgcgagaaaa ctatggaatt tgaacaattg ctaaccaatc tccttaggac
    10741 agcccgtcgc ggtaaaatcc ttcgtgaagg aatttccact gccatcatcg gacgtcccaa
    10801 cgttgggaaa tctagcctcc tcaacaatct cttgcgtgag gacaaggcta tcgttacaga
    10861 tattgctggt accacacgtg atgtcatcga agaatacgtc aacatcaacg gtgttcccct
    10921 aaaattgatt gacacagctg gtattcgtga aacggatgat atcgttgaac aaattggagt
    10981 tgagcgttcg aaaaaagctc ttaaggaagc tgacctagtt ctgctagtac taaacgctag
    11041 tgaaccacta accgcccaag atcgccaact cctagaaatc agtcaggaga ctaatcgcat
    11101 tattcttctt aacaaaactg acctgcctga aacgattgaa acttcggaac tacctgaaga
    11161 tgtcatccgc atttcagttc ttaaaaatca aaacatcgat aaaatcgaag agagaatcaa
    11221 caacctcttc tttgaaaatg ctggtttggt tgagcaagat gctacctact tgtcaaacgc
    11281 ccgtcacatt tccttgattg aaaaggccgt tgaaagccta caagctgtta accaaggtct
    11341 tgaactaggg atgccagttg acttgcttca agttgacttg acccgtactt gggaaattct
    11401 aggagaaatc actggagatg ttgctccaga tgaactcatc acccaactct ttagccaatt
    11461 ctgtttagga aaataagaaa aatccatgat ccttcattcg gtcatggatt ttattgtctt
    11521 tattagtaat ctggtcttaa gacccctgtt acagttgcct tggtcgcttc gtagtcgcca
    11581 tctacgacaa ccttgataat gcgtttggca tcttcttctg gtgctggaac aagaggtaga
    11641 cgagtgggtc cagcttcaaa tcccatatag ttaagaactg ccttaactgg agcaggactt
    11701 ggataagaga agagggcatt aaccttagga atgaatttac gttgaatagc tgcggctttc
    11761 ttcatatcgc tttctgcaat ggcagtaaac atctcgtgca tttcatcccc atttgtatga
    11821 gaggcaacag aaataacccc atccgcccca aggttcatgg catggaaagc atctccatcc
    11881 tcacctgtat aaatcaagaa ctcttcaggc ttgtgctcaa tcaagtaagc catattagcc
    11941 aagctagtac attctttgac accgataata tttggatggt cagccaaacg aagcatggtt
    12001 tctggagtca attcgacaac tacacgccct ggaatgttat agataataat tggtaggtca
    12061 gaagcatctg caatagcctt aaagtgctga tacatccctt cttgagaagg tttgttgtag
    12121 taaggaacaa tagcaagccc agctgcgaaa ccaccaaatt ccgctacttc tttgacaaac
    12181 tcaatagagt cacgcgtatc attagtacct acacccgcaa tcaaaggaac gcgtccattg
    12241 acaacctttt gtacagccgc aaacaactcc aactcctcat cgtgggtcaa agttggactc
    12301 tcagcagtcg ttcctgcgag aagaattcca tccgtatgat gggccaataa atgctcaatc
    12361 aaggctggaa tagcatcaaa gttaatggaa ccatcctcat ggaagggggt aataaaggct
    12421 gtaatgattt tacattcttt taaatcttga taagacacga aaacacctct ctatttcaaa
    12481 gaaggagttc atctgaactc ctaaacgata tgactatttt aattcaaatt tcaattcggc
    12541 tgttggacga accaatccac gttcatgaag agtttcagca atctgaactg agttccaagc
    12601 agcacctttg agaaggttat ctgaaacaac ccacatgtga attccttttt ctgcatccaa
    12661 gtctttacgg atacgaccaa caaaggtatc acgcgaacca actgcattga tagcttgagg
    12721 atagatttga tgagctacat catcttcaag aacagcacct gggaaggctg cgatagctgc
    12781 ttttacttct tcgattggag ccacttcttt tgtttcgata taaacagact cagagtgagc
    12841 tgacaagact ggaatacgca cacatgttgc agatactgca atgctatcat cttccataat
    12901 tttcttagtt tccttggtca tcttcatctc ttcgtaagtg taatcattgt cagtgaagac
    12961 atcgatttgt ggaagagcat tgaaggcgat aggataatgt ttcttgtcac cgcctgaagg
    13021 taagatttcc gcatgcaaat cacgtggttt cgctccatca ttcaagacct cacgaagttc
    13081 acgttgcgtc tcaagaattg ctcccatacc agcacctgaa acggcttgat aagttgaaac
    13141 aatgatacgg tccaagcccc atttttggcg aactggctca agagctacca tcatttggat
    13201 tgttgaacag ttagggcaag caataatccc gttgtgggca tcaagtgcgt gagcattgac
    13261 ctctggaaca accaaaggaa cgtctggatt ttgacggaag taagatgtat tatctactac
    13321 tactgcgcca gctttaactg cgtatggtgc atacttagct gatgtcgaac cacctgctga
    13381 aaaaagagca atatcaactc cttcaaaagc tgtttcagtc gtttcttcaa tcgtaatatc
    13441 ttgatcttta aatttcaatg tcttgcccgc tgaacgagca gaagcaaggt aacgaatttt
    13501 atcaattgga agtgttgatt cttccaacat ttttatcatc tgagctccga cagcacctgt
    13561 cgcgccgact acagcaactg tatatcccat aaataacctc tttcggaatt ttctaaaaat
    13621 ttctataata gaagtattat actacttttt ccatgaattg caaagctttt tcatcatttt
    13681 ttggaaaata aaaatcccag tcgctagact agggactaag aggcatcttc atgtgatgag
    13741 caggttcaca caactcatca aggtccgctc ctgcgttatg acctccttat gctcaatagt
    13801 agtccgaaga ctacctatcg actcatcgca tattctatat tactagagat acttgatttt
    13861 gtcaataaga aattttttcg atcgagtata acaaaaaact ctcgtattca agagtttccc
    13921 tatacatatc atgctaattg ccgggattga accggcgacc tcatccttac catggatgcg
    13981 ctctgccaac tgagctaaat cagcttactt aagaagtata ctatattcag aaaaacttgt
    14041 caagtttcct tagaaatttt ccataggaaa aagccaggta agaactacct agcttatctt
    14101 cttctattta cttaaatcaa ttcgacgacc aattttacca ataataatcg ctccaataat
    14161 caatgaagca aattcaccga ttcctgttgt aaaccaagta aggaagaatg gtaattctgc
    14221 tacaatattt aactctgcag cgatagtaaa catggaaatt gaaaagagga ttgaaaagaa
    14281 gaaatgatct tttcgaatca atccgttaaa gaggtaatcc ttgctatatt ttgcaaaaag
    14341 ccaaacacct aaactgagga atactaaggt tgaaccacca ccaacaaaga catctagcag
    14401 tccgaagcta aagaaattag caatcataca accgatggta actccgatga tgtacttagg
    14461 attgtaaaaa gccataaagt tcatcatttc tgaaatacga aactgataag caccatagct
    14521 gatggcattt aggggcggtg tgacagtcaa aacgacgtag atagcagcga cgattgcaat
    14581 atctgcaaca tcacgaatag ttaatttttt catcttttct cctttggcgg ttatccgcgt
    14641 gtaatatgct tggtgaaaga agctaagcac caagggttga ttcattcaac cttactagta
    14701 tagcacaata gcctgcttta tgctatactt aaagcatgaa aaaacctatt caaaaatttt
    14761 ttaacaatga aatcttagcc tatctctttt ttggccttgc gacaactcta gtttcgattc
    14821 tctcacgact agtcatttat caactaagtc atcaggaact ccttgctacg gccttggcaa
    14881 atattatcgg tatcttattt gcctttatca caaatgatac gattgtattt aagcaaaaaa
    14941 agaaaaatag acttatccgt ttagtgaaat tttctcttgc ccgcctttct acttttctgt
    15001 tagacttgtt tttaactttt ctctttgtaa cccaatttcc tcacatcata gggcaattcg
    15061 taactgaaaa cattgataag ataaatgcta ttgaaacaat aattgcacaa atattcatta
    15121 ttatcatcaa ttatatcttg agcaaggtct atatttttag aaaataatgc tccatttatg
    15181 agccttttaa ttgccttcta taatgattta ggatacaatg aaacacgaaa cattgaaagg
    15241 aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt attgaccttg
    15301 ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt tacttcactt gtaaacgaca
    15361 ttatcactcc tcttatttta aatcccgctt tgaaagctgc taaagttgaa cgtatcgctc
    15421 aactttcttg gcatggagtc ggctatggta acttcttaag tgctattatc aattttatct
    15481 ttgtgggtac cgccctcttc tttattatca agggcattga aaaagcacag aaactgactg
    15541 gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga attggaagtc cttcaagaaa
    15601 taaaagctct ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa gctaaatcct
    15661 tttttcttta ctctttgggt aacttgcctg ctttttcaag catttttttg ataagataag
    15721 gcatcttaac attctcccgc ccttttttct caatcagttt tttcacaaat tctggcattt
    15781 gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc tgaaggaact aactcatcaa
    15841 aggtctcttc ttctgggaga aattccttaa actcttgatt ttcattgact ctaactgtat
    15901 tgaccaaagc atcaatcaaa cgagaatctg tatttggcat tggtggacga tggtagttta
    15961 ccccaaattc ctggcataag tcataacatt ctacatcatt gtcaaacaag acctcaatat
    16021 gctcactgat gaaactaata ggtacaaaaa tataatggtc tggatgttct gtctgttctc
    16081 tgagatactc caaaacatct ggtttaatcc atggaatacc aatatcactc tcactctgcc
    16141 aagtgttggt atattgttct gaactcaagc ccagtttttc agcgactagc ttgctatttt
    16201 cgaaaatttg gtctatataa ggatcaccaa agtccaaggc aaaaatgggc acactatggg
    16261 ctgaaaagat gactttaaag ctgtcttgct ttacttcttc ttttaaaatc ttagcaattt
    16321 catctgccca atagtttaag agcacttctt cctgatacca gtccttaatg accaaaaatt
    16381 gaatttgttt gctttctaaa aatttttcat agcccatgac agagtagaaa gaataatgtg
    16441 gctccaaaat caagcaaata cactgttcaa ttccgtctgc ttccatttga ccgatcacat
    16501 ctggaataaa gggactggaa aacttattag caaagtagac actgtattca ttacccagcc
    16561 tagcttctac caaggtaacc tcttgacggg taattttttg cagaggcgtg cctccaattc
    16621 gtacataatt atcatagaga gtctgaatct cgtggtcttg gggtctcacc ccacgacgaa
    16681 tatttgtgaa aaaatcagct acaccttcaa aggtaatctc ttctggtgaa ccgaaggtca
    16741 tcattaaaat tgcttttttc atgactgctt ccttgtgatg tttttatcaa gtttatttta
    16801 tcattaattc atcgataagt aaacgctaac atagggacat tctcaatctt ctgtcttttg
    16861 tttttctctt ctttctatga tacaatggaa aaaataaatt caaaaggagt ttttttatga
    16921 cttatccaaa tctcttggac cgcttcttaa cctacgttaa ggtcaacacg cgctctgatg
    16981 aacactctac tactactcca agtacacaga gtcaggttga cttcgcaaca aatgtcctaa
    17041 ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta tctaccgaat ggttttgcta
    17101 ttggaacctt gccagccaac gatccgtctt taacacgtaa gattggtttt atatcgcaca
    17161 tggatactgc tgattttaat gctgaaggag tcaatccaca ggtaattgaa aactacgatg
    17221 gtggtgtgat tgaactaggg aattctggtt tcaaactcga tccagctgac ttcaagagtc
    17281 ttgaaaaata tccaggacaa acgctcatca caacagatgg aacaaccttg ctaggtgctg
    17341 atgacaagtc aggaattgct gaaattatga cagccattga atatctaact gctcatcctg
    17401 aaattaagca ctgtgagatt cgtgttggtt ttggtccaga tgaagaaatc ggtgttggtg
    17461 ccaataaatt tgatgcagaa gattttgatg tggattttgc ctacactgtt gatggtggtc
    17521 cactaggtga acttcagtac gagactttct cagccgctgg tgctgaattg catttccaag
    17581 gccgtaatgt ccaccctggt actgccaaag ggcagatggt caatgccctt cagctagcaa
    17641 ttgattttca taatcaactt ccagaaaatg accgacctga gttaactgaa ggttaccaag
    17701 gtttttacca tctaatggat gtgacaggta gtgttgagga ggcgcgtgca agctacatca
    17761 ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc atccatgcaa tctatcgctg
    17821 ataagatgaa tgaagaactt gggagcaacc gtgtcactct caacttgaca gaccagtact
    17881 acaatatgaa agaagtcatt gaaaaagata tgactccaat taccattgct aaagccgtta
    17941 tggaagatct aggtatcacg cctattatcg aaccaatccg aggtggaaca gacggctcta
    18001 agatttcctt tatgggaatc ccaactccga atatctttgc aggtggagaa aatatgcacg
    18061 gacgttttga atacgttagc cttcagacta tggaacgtgc agttgatacc atcattggca
    18121 tcgtacgtag cttataaaag ctaaaaagac gaggtagctc agctacttcg cctttctttt
    18181 tattctactg gtttttcttg atttccagta gttgtagaag attctgttgt tttattttct
    18241 gaagttgatt cagcaggttt agaatctctt gtattgcttg gtttgttttc gtcgctagca
    18301 gtttcaatgt tagattctgc agttgcgttt ggttggttct cagcactggt gttatcacca
    18361 tttgcttcag catttcttgc tggacttgtt tcttcacttg cgctagcttt tgactggatt
    18421 tgatgattca aaactagaat agcttttgtc gattcaagta aagctgtttt gtctttactc
    18481 ttagcagaaa gttgatctaa taatgcatcc accttatcaa agtccgcatc agatccatta
    18541 ttactttcta aataagagtg aagcgacatg agaatatcgt agagtttttg atagagtaca
    18601 agtgtctgag gatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga
    18661 gtcaaaacat cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg
    18721 gcagctttta gattttctac ttcttctgcc aaggattgtc tgattccttc ttcatggatt
    18781 tgttccaaga gttgatttgc cttgttcaaa agactttcta cttcttcctt gctatctgtc
    18841 gcagattatt ggttgctatc taccatgtac tcctaaaaca ggagagttat aatccaagat
    18901 tacaaggcct tacagaaata agaaatccag ataagacaat gttcgtccaa gacgctattc
    18961 gcttcgcaca gcagcacgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct
    19021 ctttttagtg tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa
    19081 actttcagta aactgaccta aagctaactc aatctgtctt tgtagatgct tctgctatca
    19141 gctagaagtt gatctacttt tgccaagact gccttctcat caaaagttcc aggttgatag
    19201 ttggattgca gggatggaat cttgtttttc aaagccgctt catatccctt agtttgaacc
    19261 ttgatgtagt gattgtggtc gccatgagga atcacaaaac cttctgaatc ttcacttata
    19321 attcgattgg catcaaaaac catgaccatc ttcttcctca tggtggacat gtagtgacgg
    19381 attacttaat acagaactag aagaacttcc tacctcttcc gtgttagagt gtgatggggg
    19441 attgttaaga gatgacttag gaatatagtg atagtgttcc ccatgtctta ctatataagc
    19501 atcacctata tctctgacaa tatcattagg gttaaaaaca tatgtggctg ctaattcacc
    19561 tgccgacaag tcactctcag gaatgaaatg atagtgacca ccatgtggta ctatagtagg
    19621 ttgaaataga atatgagcaa attgataagg ggattttaaa gtaatttcta acaatgattt
    19681 agaaactatg atgtgctatt ctaaattcaa ctcactatat ataaccatca tcggtagtat
    19741 aacgtccctg taattttgct acagatactt ctgcactagc tcctttatcg tctttaccat
    19801 gttcttgttt ttggcgattg atttcatctt ttgttcgtac attttctgca tgagcttaat
    19861 ctttaaggta aacataatac tttccatcta ccttaatact atatcctccc ttaacctaac
    19921 tgacgatatc ttgatctttc ggctgatagt tgggggcttt cattaatggc tcttcactaa
    19981 agagcgcatc aaaaggaact ttaccattat agcagtgata atgatcgcca tgagaagtta
    20041 cataaccttg acctgtaatc ttaataacaa tttgttttgc ttgaattcct tctttttgac
    20101 taacctagtc tggagtcaaa ttttcagtct tcttagtgtc tttattactg tttacatatg
    20161 aaacacgatt tttatctgta ttggcctgtt agctatgttg gttcagagca taaacacaca
    20221 gacttaagga aaggataaca
//
LOCUS       DAVKZB010000026        18695 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKZB010000026 DAVKZB010000000
VERSION     DAVKZB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.026"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(158..964)
                     /gene="yidA"
                     /locus_tag="VXA80_000865"
     CDS             complement(158..964)
                     /gene="yidA"
                     /locus_tag="VXA80_000865"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV0353389.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTLVL"
     gene            complement(977..2281)
                     /locus_tag="VXA80_000866"
     CDS             complement(977..2281)
                     /locus_tag="VXA80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0353390.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2353..2730
                     /locus_tag="VXA80_000867"
     CDS             2353..2730
                     /locus_tag="VXA80_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV0353391.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2821..3153
                     /locus_tag="VXA80_000868"
     CDS             2821..3153
                     /locus_tag="VXA80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV0353392.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3165..4736
                     /gene="ffh"
                     /locus_tag="VXA80_000869"
     CDS             3165..4736
                     /gene="ffh"
                     /locus_tag="VXA80_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV0353393.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4931..6214)
                     /locus_tag="VXA80_000870"
     CDS             complement(4931..6214)
                     /locus_tag="VXA80_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV0353394.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6517..7230)
                     /gene="rsmG"
                     /locus_tag="VXA80_000871"
     CDS             complement(6517..7230)
                     /gene="rsmG"
                     /locus_tag="VXA80_000871"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV0353395.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7324..7884
                     /locus_tag="VXA80_000872"
     CDS             7324..7884
                     /locus_tag="VXA80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV0353396.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            7886..8785
                     /gene="htpX"
                     /locus_tag="VXA80_000873"
     CDS             7886..8785
                     /gene="htpX"
                     /locus_tag="VXA80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV0353397.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIILLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8934..10493)
                     /locus_tag="VXA80_000874"
     CDS             complement(8934..10493)
                     /locus_tag="VXA80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0353398.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQFLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10732..11049)
                     /locus_tag="VXA80_000875"
     CDS             complement(10732..11049)
                     /locus_tag="VXA80_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV0353399.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            11219..11761
                     /locus_tag="VXA80_000876"
     CDS             11219..11761
                     /locus_tag="VXA80_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV0353400.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFASLQGLFDGDE"
     gene            11761..12390
                     /gene="nth"
                     /locus_tag="VXA80_000877"
     CDS             11761..12390
                     /gene="nth"
                     /locus_tag="VXA80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV0353401.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNPFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12601..13122
                     /gene="pyrR"
                     /locus_tag="VXA80_000878"
     CDS             12601..13122
                     /gene="pyrR"
                     /locus_tag="VXA80_000878"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV0353402.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13141..14064
                     /locus_tag="VXA80_000879"
     CDS             13141..14064
                     /locus_tag="VXA80_000879"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV0353403.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14114..15193
                     /gene="carA"
                     /locus_tag="VXA80_000880"
     CDS             14114..15193
                     /gene="carA"
                     /locus_tag="VXA80_000880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV0353404.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15506..18682
                     /gene="carB"
                     /locus_tag="VXA80_000881"
     CDS             15506..18682
                     /gene="carB"
                     /locus_tag="VXA80_000881"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV0353405.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5430 a         3624 c         4193 g         5448 t
ORIGIN      
        1 caattcagta ccacaaatta agaaagcaat gcctattttt tgtactctca agatttaagt
       61 aactgtacaa ctgcaattaa atttattaat ttactctcat tgtgatatat tcatacaaaa
      121 accgctcagc tgagcggttt tagtctattc tttcctttta cagtaccaat gtacgaatag
      181 catgggcaac gccggattcg tcatttgttt tggtgatgta tttggcgatt tttttgattt
      241 ctggatttcc gttttccatg actacagggt tgccaacgac ttccagcatg gcacggtcat
      301 tttcttcgtc tccgatagcc atggtttcat ctttggttaa tcctagtttt tcagctaagt
      361 gagtaatagc tgaacccttg tctacattct ttttaaggag ttcgaggtag aaaggagcag
      421 atttgttgat agagtagcgc tcgtaaaatt ctgctggaat tttttcaatc gcagcatcga
      481 gaatttctgg ttcatcgata aacatacatt taacaatttc tttgccagcc atttcttcag
      541 gggtacggta gaagataggc atgctgacga gggttgattc gtgtacagtg tattttccga
      601 tattgcgatt tgcagtatag ataccgtcct tggtaatggc atgcatgtgg acaccgagct
      661 tgcgactgag gaattccata tctagataat cctcataagt caaggattcg ctgataatct
      721 catgtcctgt agcagtttct tggacaaggg caccgttgaa ggttaccaca tagtccccct
      781 cgtctctcaa ctgcaagtcg tctagaagtt tggcaacgcc tgcgataggg cggccagttg
      841 caatcacgac tttgacacca gcttctttgg catcttggat ggcagaaaaa acttcaggag
      901 tgatttcctt ttggctgttg acaagggttc cgtcgatatc aacggcaatt agtttaatac
      961 tcatacattt cctcttctag tttttatttg gggtaaaatg atcgttctca atcaagtgta
     1021 aaaattgctg ggtaatgctt gcaaagatgc tgttttggtc caacatttct tttggaaaat
     1081 aaaagcgatt atctccgtgg cgactgccag caagggattg gacgatagga gacaggctag
     1141 agagttcggc cagttctcca tttttttgta aaatctcaat ctgtgtccgt gggttttcag
     1201 attcgggacg atagatatca taagggaggt caaagttctt atgaatggca gtgtagtagt
     1261 cgggatcaaa gccgatatcc tcaaccaatt ttctcatgct agtaagttga tcttggtcct
     1321 cttgtgaaaa ggtaatggat ttaaagacct tgcggttgac aaagcgatgc gataaatctg
     1381 caagaatctt gtcaggactg gtcatccaaa gctggaagta ggtattcatc acgccatcat
     1441 ccagagccag atagtcagtc aaggtcacat ttttttcgaa gaaaggcagg aggtgtggag
     1501 aagttcgggc aaagaaatcc ttgtcctcag gatagagttc cttggcgcgt ttgagaagat
     1561 tctgtaggag aacttccatg gcgcgtgttg cggggtggaa ataaacctgc atgtacatct
     1621 ggtagcgact gaggacgtag tcttcgatgg cgtgcatgcc attgcgctga aaggcgatac
     1681 cattttcgat aggacgaatg actcggagga ttcgagtcag gtcaaattcc ccataggatg
     1741 ctcctgtaaa ataggagtcg cgcaagagat agtccatgcg atctgcgtca atctgactag
     1801 aaatgagctg cacgacctgc ttattaggat aggtatggtc aatgacactg gccacctttt
     1861 ctgggaaatc aggtgccact tgtagcagga cttggtgaat ctctgtctca ggattttgaa
     1921 taatctcctg agtaatggct tcatggtctg tatcaaagag atgttcaaaa gtatgggagt
     1981 aggcaccatg cccaaggtcg tgtaggagag cagcggtcat ggtcaagaga gactcggcag
     2041 gattccattc ctcaggatat ttttcttcga aaatctctgt gatgcgtcgt gcaatttcat
     2101 agactcctag acagtgagag aagcgactgt gttctccacc gtggaaggta taactggaag
     2161 ttcccagttg tttgatccgg cgcaaacgct gaaattcttt tgtattaatc aagtcataga
     2221 tgatttgatt attgacatgg atgtagttgt gaactgggtc acggaatact ttttcgttca
     2281 tatgaccatt atagcaaaat cctgctcttt ttggtaaaat agtaggacaa gagacaagaa
     2341 agagggcctg atgtgaagat tcggatgcga aatacaattc agtttgatga gcagttggaa
     2401 gtgattgacc agctttatga tgtggaagtg catgaaaaag gagattatag ctacctgctt
     2461 ttctataatg aggaaaagga aaaagtagtg attaaatttc atggtcaaga acttatgatg
     2521 agccgatttt ctaatcccaa gaccattatg cgctttctaa aggatagcga tagtttagcc
     2581 tatattccta cacctatggg catgcaggag tttatcatcc aaacgagcca ttatcaagtt
     2641 gataggcaaa agattgaact agattatcaa ctacaaaatc aagagggaca tccttttgcc
     2701 agctatcaat tggaaattac ttggggctag gctcatgtct ttttcaaatt tagctatcta
     2761 tcttcttgga tcggcttcct agtgtggtag attaggctaa tttttggtat aataaaagtt
     2821 atggaaatcg aaaaaaccaa tcgtatgaat gcgctctttg aattttatgc ggcgcttttg
     2881 acagataagc aaatgaatta tatcgagctc tactacgctg atgattacag ccttgctgaa
     2941 attgccgagg agttcggtgt cagtcgtcag gctgtctatg acaatatcaa gcgaacagaa
     3001 aagattctgg aagattatga gatgaaattg cacatgtact cggattatat tgtccgcagt
     3061 cagatttttg atcagatttt ggagcgctat cccaaggata actttctgca ggagcagata
     3121 gaaattttaa caagcattga taatagagaa taagaggaag aaaaatggca tttgaaagtt
     3181 taacagaacg tttgcagaac gtctttaaaa gtctacgtaa aaaaggaaaa atctctgaat
     3241 ctgatgtcca agaggcaacc aaagaaattc gcttggcctt gctcgaggcc gacgttgcct
     3301 tgcctgttgt aaaggacttt atcaagaaag ttcgtgagcg tgcagtcggg catgaggtca
     3361 ttgatacact taatcctgcg caacagatta ttaaaatcgt tgatgaggaa ctgacagctg
     3421 ttttaggttc tgatacggca gaaattatca agtcacctaa gattcctaca atcatcatga
     3481 tggttggttt gcagggagct ggtaaaacaa cttttgcagg taagctggct aacaaactca
     3541 agaaggaaga aaatgcccgt cctttgatga ttgcggcgga tatttatcgt ccagctgcca
     3601 ttgaccagct taagaccttg ggacaacaga ttgatgtgcc tgtctttgca cttggaacag
     3661 aagtaccagc tgttgagatt gtacgtcaag gtttggagca agcccaaact aatcataacg
     3721 actatgtctt gattgatact gcgggtcgtt tgcagattga tgagctcctc atgaatgagc
     3781 ttcgtgatgt gaaagcattg gctcaaccaa atgaaatctt gcttgtcgtt gatgctatga
     3841 ttggtcagga agcagccaat gttgcgcgtg agtttaatgc tcagttggaa gtgactgggg
     3901 tcatccttac caagattgat ggcgatactc gtggtggtgc tgctctgtct gttcgtcaca
     3961 ttactggaaa accaatcaag ttcactggta caggtgaaaa gattacggac attgaaacct
     4021 tccacccaga ccgcatgtct agccgtatcc ttggtatggg ggatatgctc actttgattg
     4081 agaaagcttc tcaggaatac gatgaacaaa aagcccttga aatggctgag aagatgcgcg
     4141 aaaacacctt tgattttaat gatttcatcg atcaattaga tcaggtgcaa aatatggggc
     4201 cgatggaaga cttgctcaag atgattccag gtatggccaa caatccagcc cttcaaaaca
     4261 tgaaggtgga tgaacgccag attgctcgta aacgtgccat tgtgtcttcg atgacacctg
     4321 aagagcgtga aaacccagat ttgttaaatc caagccgtcg ccgtcgtatt gctgctggtt
     4381 ctggaaatac attcgtcgaa gtcaataaat tcatcaagga ctttaaccag gctaaacagc
     4441 tcatgcaggg tgttatgtct ggggatatga ataaaatgat gaagcaaatg gggattaatc
     4501 caaataacct tcctaaaaat atgccaaata tgggaggaat ggatatgtct gcccttgaag
     4561 gaatgatggg acaaggcggt atgcctgact tatcagctct cggaggagca ggaatgccag
     4621 atatgagcca gatgtttggt ggcggtttga aaggtaaaat tggtgaattt gccatgaaac
     4681 agtccatgaa acgtatggct aacaaaatga agaaagcgaa gaagaaacgc aagtaagaca
     4741 agagattgat gcaaatctca tttgatgaga aaataaagta aaaatttcta tgataaaacg
     4801 tacagtatca aggttttaga atatctgata ttatacgttt tttgatttta ataattttgt
     4861 taagacactt ttattatggt atgcgaaaaa tccactcagc tgtctgagtg gattaggtgt
     4921 atttagactc ttagtcttta ttttcgtatg gcaagatcaa gttcaagatg attcctgtca
     4981 tggctgaaag ggcagtacct gaaagtgtaa ctggaccaag tttaaggata gctcctccaa
     5041 gtccaagaac caacatagca cttgcgatga tgaggtttcg catttgagcg aaatcaacac
     5101 gttctttaat caagactttc aaaccattgc tggcgataac cccatagaga aggattgaca
     5161 taccaccaag tacagcgttt ggaatagttg aaatcaaggc agtgaattta ccaaggaagc
     5221 tgagggcaat cgcgatgaag gcagcgttac ggataactga gacagaagcg atacgagtca
     5281 taccgataac ccctgtattt tctccgtaag ttgtattggc tggtccacca aggaaggcag
     5341 aaacagaagt tgcgataccg tcaccaataa gagtacggtg aagacctggt tcttttaaga
     5401 attgacggcc acagatttga cccaaaacag tatggtctcc gatatgttca gaaattgtta
     5461 cgatagcgat tggcaagata gcgatggctt ctggaccaaa gtaaagattg tactctttaa
     5521 aggcaccacc tgtgctaaat ggcaagtaga aaccaggaat ttcaaaccag ttagcttcta
     5581 ggacaggcgt aaagtcaacc aaaccaagag ttagtgcgaa gaggtaacca ccgataatgg
     5641 caaagaggaa tggaatgatt cgtaggaagc cttttccttt tgtattgata aaggcagcaa
     5701 ttaggaaagt aacaacggct accagagcat ttttccaatt tccgtctgct acaagacctg
     5761 cattggtaac agctgaacct gcaagtccaa gaccgataac gatgatcata ggaccgataa
     5821 tgattggtgg caagagttta tcaatccatt ttgttcctac aaatcggatg ctggtagcaa
     5881 caaggacata gaccaaacca gtcaagataa cccctgtttg agcagcagat acatcccccc
     5941 ccatttcttt catagccagt gacatagctg tgataaaggc gaatgaagaa cctagataaa
     6001 ctggaacttt aaaaccagta gcaatcatgt agatgagtgt tccaacacct gaagcaaaaa
     6061 gggcaacaga tacaggcatt cccaaaatca atggtaccaa gatggtcgca ccaaacatgg
     6121 cgaaaacgtg ttggaagcta aggagaattc cttttccatc cgaaggacgt tggtcaacgt
     6181 ctagtaacaa atcaacagtt gattcttgtt tcatataaac ctcacttttg ggcatcagaa
     6241 aagagcccat tattttacag caataggccc tcagagtaag acttctctaa aatctagact
     6301 ttaaagagtt tcaaatattt ttgcgtcttc gtcacctcac gggatgacat taaaaacctt
     6361 tgcttaggat agtatagcag aaaaaaatga ttttgtaaat cattttttcc cgagcctaga
     6421 aataaaagag cgaggctgat tttgtaaatc attttttccc gagcctagaa ataaaagagc
     6481 gaggctgatt ttgtaaacat ttgtttacta aaaaatttaa agtgggcgtt tatttggcat
     6541 accagcctta cgtggatatt tatttggtgt ttcttttttc ttttctacca ctgtgatata
     6601 gcgcggatct ctattcggta gggcgtagct gagattgtct tcgaccttac taaaaaggag
     6661 attgagggca ttcttagctt ctaataattc ctcaggcgca ttgctggcct tgagtgccaa
     6721 tagtttgcca ccaaccttaa ggtagggaat agtcaattca gataggacct gcatacgggc
     6781 aaccgcacga gctgttacaa aatcatattg agcacggaag ttcttgtctt gggcaaaatc
     6841 ttcggcacgt ccgtggtaga aatgaactcc gttcaaatcc agttcttgag ccaagagttg
     6901 taggaagttg atgcgcttat tgagtgaatc aataatggtc acatctaact ccggatagag
     6961 aattttcatt ggtagactag gaaatcctgc cccagccccg atatcaagaa gtttgatagt
     7021 ttcattggga atcaaacctt gaagaatggg tgcaatcgaa tcgtaaaaat gtttgagata
     7081 aacttcttcc ttgtccgtaa tcgccgtcaa attaatcttc tcattccact cgaccaagag
     7141 ctcaaaataa cgttcaaatt gttctttttg ctggttcgaa agtggaagat tctgctcggc
     7201 aagcaagttg taaaatgttt ctggtttcat agttatttcc ttctttagta tcatcttatt
     7261 ttaccacaat cattaaaaat ttgatatact ggtactaatc taaaagcaga aaggacttat
     7321 attatgactt ggattattct tggagttatc gctcttattg ttatttttgt gattgttagc
     7381 tataacggtt tggttaaaaa tcgtatgcaa accaaggagg cttggagtca gattgatgtt
     7441 cagttgaaac gtcgaaatga cctcttgcca aacttgattg agactgtaaa aggttatgcc
     7501 aaatatgaag gttctaccct tgaaaaggtg gcagaactac gtaaccaagt ggcggcagcg
     7561 acttcaccag cagaagctat gaaagccagt gatgccctca ctcgtcaggt ttcaggtatt
     7621 tttgcagttg cagaaagcta tccagatttg aaagctagtg ctaactttgt taaattgcaa
     7681 gaggagttga caaacacaga aaataaaatt tcttactctc gtcaactcta taacagtgtt
     7741 gtcagcaact acaatgtaaa attagaaact ttcccgagca atattatcgc tggaatgttt
     7801 ggatttaaag cggcagattt ccttcaaaca tctgaagagg aaaagtcggt tcctaaagtt
     7861 gattttagcg gtttaggtga ctaagatgtt gtttgatcaa attgcaagca ataaacgaaa
     7921 aacctggatt ttgttgctgg tatttttcct actcttagct cttgttggtt atgcggttgg
     7981 ttatctcttt ataagatctg gacttggtgg tttggttatc gcactgatta tcggctttat
     8041 ctacgctttg tctatgattt ttcaatcgac agagattgtc atgtccatga atggagcgcg
     8101 tgaggtggat gagcaaacgg caccagacct ctaccatgta gtggaagata tggctctggt
     8161 cgctcagatt cctatgcccc gtgttttcat cattgatgat ccagccttaa atgcctttgc
     8221 gacaggttct aatcctcaaa atgcggctgt tgctgcgact tcaggtctac tagctatcat
     8281 gaatcgtgaa gaactagaag ctgttatggg acatgaagtc agtcatattc gtaattatga
     8341 tatccgtatt tcgactattg cagttgccct tgctagtgct atcaccatgc tttctagtat
     8401 ggcaggtcgt atgatgtggt ggggtggagc aggtcgcaga cgaagtgatg atgaccgaga
     8461 tggaaatggt cttgaaatca ttttgctagt ggtttcccta ctagctattg tactggcacc
     8521 tctcgctgca accttggttc agctcgctat ttctcgtcag agggaatttc tggcagatgc
     8581 atctagtgtc gagctgactc gcaatcccca gggaatgatt aatgccctag ataagttgga
     8641 caatagcaaa cctatgagtc gccacgtcga tgatgctagc agtgcccttt atatcaatga
     8701 tcccaagaaa ggtggggggt tccaaaaact cttttatacc cacccaccta tctcagaacg
     8761 gattgaacgt ttaaaacaga tgtaaaatga aggctggaaa aaagtcttta aaatctgaaa
     8821 aacgcataat atcaggtgtg aaaacttgat attatgcgtt ttactatggg aagatttact
     8881 tctttttctc ctaaaattgt gtttttgccc cacctatctg ctatgttgca aatttataaa
     8941 tcttctaaat taactagctt caaaccgtgt tctgtcatgc gatagatgat cgagcagact
     9001 tcttttaaga aacgacggtc atgcgaaaca gtgatgagac cgcctggata ggttgcaaag
     9061 agttttctga tttggggttg agaagtggga gaaaagtttc gtgtgggttc atccagcagg
     9121 agaaagtttg gtttgcgcag gactaaatcc aaaagcagga gttttccctg ttgtccgcca
     9181 gataaggagc gaatttgatg ctgcatttct ggataactga aattgagact ggctaggtga
     9241 gattggattt tctgtagttc ctctttttcc ccagttttgc tgaggtaggc tattggtgat
     9301 aaatccaatt gcagtttttt gtggtaatct tgtggcataa aaccaaggga aatctctctt
     9361 ttatcattca gaaattgctt taatttggct aacagagttg atttcccaac accatttggc
     9421 ccgataatac cgattttttc ttgcccacgg acagttagtt gtagtttttg aaccaaaact
     9481 cggtcgtcaa tggacaaatt ttctttttcc agttggacta agactttaga agctggtaat
     9541 ggttggatgt ctgaaaagaa aagttggatt tgttcctctt caagtggttt ttgagtcatg
     9601 gactgagctg ccttttcgta gcgtttttct tgtgagagga cagttttcat ctttttagac
     9661 aataggcgac cggcagtact atctttggta gctcgaagcg cagtttctac attttgctta
     9721 actctccgat gtttttccat ggttttatcg taggctcttt ggttattagc agcttgctga
     9781 ctttgtttgg caaaattagc ctttctctgc tcactatagc tatcatagtc taaatgctct
     9841 actagcgttt ccgcttcttt acggtgtttg accagtcgca agtgaacaat agtgtctgcc
     9901 gtttcagaaa gaaagtcttc atcatgggaa atgaaaataa cggtttgcct ggtcttttga
     9961 atctggcctt ttagccaatc aactgtctca aggtctaggt catttgaagg ttcatctaaa
    10021 aatagaatct caaagggttt ggctaactca tggataagct gaattttcaa agcttctccc
    10081 cctgatagat tgccaatctc ttggtcactt gcgaaacgat tgctatcaaa atgcaattcc
    10141 tccgccaaac gatagaggat actgtagtct aaatcaatag aatctaaaaa gaagtagtcg
    10201 tgtaaagttt tctttttcag ctcctcggga agtttttgag gaatgtaggc cagtgactga
    10261 tagtcagatt ggatgtttcc cttgatagtg aaatcagaca aagcttcccc cattaaaatt
    10321 ttaagtaagg ttgatttgcc atttccttct tcaccaataa tagcaacctt ttccccgtct
    10381 tggatggtca tggttaggtc agagacaagg tctcgtaaat ctttgttttg tgtgatggtc
    10441 aggtgattga tttttatcat atgatttccc tttctagaat gttcatagtg cataacaaaa
    10501 agctctactt tacctagtag agcccaaagt ctatgtcaaa aaaactctat taacatgcca
    10561 gaaaaagagc ataaaagagc acacaaaaag agacaaaaac aagatctact aaataaaggt
    10621 tctttatttg atagacttag ttgttcatgt ctcccttacc tccgagtatt agtatcttta
    10681 tactatacct ttaaggaaat tttgtcaaca gaaaactgga aattctatgc tctaaaatcc
    10741 aaaaacaggt ccatagagac ttagtacgtg tttgacatgc tcgtaatgga ttttatcata
    10801 gtttcgccaa gcagtgcgtg cttgattgtt tagttttagg ctacccagtc caatcagagg
    10861 actggtgggt tggtaaaatt tctcaagatc aattaagata ctgttgttca actgttccgt
    10921 ttttttgagc tcttgcatag tagtgtttag cagctcttgt agtcgaatat catctgccgt
    10981 accctgttta cagttttggt agcttttatt taacttggat aggagttgaa tgagtttctt
    11041 gtcttccatg tgagcatcct ccttgatctg atgtactctt tccctatagt atagcactaa
    11101 aaaacaaaac tataatagta aatatgttaa aattttgaat tttaattaca aagtttggtg
    11161 tgctgatttg tagccttttc atggtataat caagagagta aatctttatg gaggaagtat
    11221 gaaattaaat attcaagaaa ttcgtaagca gtctgaaggt ttgaactttg aacaaacgtt
    11281 agatttagtt gatgacctgc gtgcacgtaa tcaagaaatt ttagatgtaa aagatatcct
    11341 tgcagttggg aaagtacaat atgaagaccg tatgtatttc ttagattatc aactatctta
    11401 taccattgtt cttgcttcga gtcgcagtat ggagccagtt gagttagttg aatcttatcc
    11461 agtcacggaa gttttcatgg aaggcgcaac taaccatcta aatcaagaaa ttttagatga
    11521 tgacttggtc ttgcccatcg aaaatggggc gcttgacctt gctgagagtg tatcagacaa
    11581 tatcctgcta aacattccta tcaaggtctt gacggctgaa gaagaagctg gtcaaggatt
    11641 tgtctcagga aatgactggc aaatcatgac agaggaagaa taccaagctc aacaagcagt
    11701 aaagaaagaa gaaaacagtc cttttgctag cttacaagga ctatttgacg gagatgaata
    11761 atggtcttgt caaaaaaacg agcacgaaag gtgctagaag aaatcattgc cctcttccca
    11821 gatgccaagc ctagtcttga ttttaccaat ccttttgaac tcctggttgc ggtcatgtta
    11881 tcagcccaga cgacagatgc agcggtaaat aaggccacac caggtctctt tgttgccttt
    11941 ccaacacccc aagctatgtc tgtagcgaca gagagtgaga ttgcttcaca tatttctcgc
    12001 ttgggattgt atcggaataa agctaaattc cttaaaaaat gtgcccaaca gttactagac
    12061 gattttgatg gtcaagtccc tcagacacgt gaagaattgg agagtttggc aggtgttggt
    12121 cgcaagacag ccaatgttgt catgagtgta ggatttggga ttccagcctt tgcagtggat
    12181 actcatgtgg agcgtatttg caaacaccac gatattgtca aaaaatcagc gacgccactt
    12241 gaggtggaaa agcgggtcat ggatatcttg ccgcctgagc agtggttagc tgcccatcag
    12301 gccatgattt attttggaag agccatttgc catccaaaaa atccagagtg tgatcagtac
    12361 ccacaattat atgattttag caatttgtaa gatgctacat tgaaactttt tgcttgattt
    12421 taagcatgct ttgtgctata gtaaatgata actaaatatt cctttaaacc tgtcccgtga
    12481 ggcaggcaag gagcgcgata aagtagaagg gtgaagtcta tactgtgtgc agtagggctc
    12541 gcttggctat aaatttcaag tctccttgtt taaggagact tttctttttg gaggtttgtc
    12601 atgaaaacaa aagaagttgt agacgaattg accgtcaaac gagcgattac gcgtattact
    12661 tatgagatta tcgaacgcaa caaagatttg aataagatcg tcttggctgg tattaaaact
    12721 cgtggtgtct ttattgccca ccgaatccaa gaacgtttga agcagttaga aaatctttca
    12781 gttcctgttg tggaattgga tactaaacct ttccgtgatg atgttaaaag tggagaagat
    12841 acttctttgg tttctgtcga tgtgacagac cgcgaagtta tcttggtgga tgatgtgctc
    12901 tatacaggtc gtaccatccg tgctgctatt gataatattg tcggtcatgg tcgtcctgcg
    12961 cgtgtgagtt tagcagttct agtcgatcgt ggacatagag aattgccaat ccgtccagat
    13021 tacgttggaa aaaatatccc aaccagtcgt tctgaagaaa tcatcgtaga gatgacagaa
    13081 cttgatgacc aagacagagt tctgattact gaagaagctt agaaagttaa aggagtagcc
    13141 atgtcagaaa atcaacaagc attgaaccat gtggtgtcca tggaagacct cactgtcgat
    13201 caagtaatga aattgatcaa gcgaggaatt gagtttaaaa atggagccca gcttccctat
    13261 gaagaccatc cgattgtttc caatctcttc tttgaggatt ctacacggac acataagtcc
    13321 tttgaagtcg cagagattaa acttggattg gaacgacttg actttgatgt gaagactagc
    13381 tcggttaata agggtgagac actttatgac accattttga ctctgtctgc tttaggagtg
    13441 gatgtctgtg tgattcgcca tccagaggtc gactactata gagaattgat tgctagtcca
    13501 acgattacga cttccatcat caatggtgga gatggttcgg gccaacaccc tagccagagc
    13561 ttgcttgatt tgatgaccat ttatgaggaa tttggccact ttgagggtct taaagttgct
    13621 attgcaggtg acttggacca ctcacgcgtt gccaaatcca atatgcagat tttgaaacgc
    13681 ttgggagctg aactcttttt cgctggacct gaggaatgga gaagtcaaga gtttgcagac
    13741 tatggacagt ttgtaaccat tgatgaaatc attgatcagg ttgatgtcat gatgtttctc
    13801 cgtgtgcaac acgaacgcca tgatagtgga gctgtatttt caaaagaaga ctaccatgcc
    13861 caacatggct tgactcaaga acgttacgat cgtttgaaag aaacagcaat cctcatgcac
    13921 ccagctccaa tcaatcgtga tgtagaaata gcagaccact tggttgaagc accaaaatca
    13981 cggattgtcc aacaaatgac caatggtgtc tttgttcgaa tggcaatctt agaatccgta
    14041 ctagcgagta gaaacgccaa ctaaaacaca agacgttaaa agacgccagt gagcgtccac
    14101 gagaggtgaa aatatgacaa aaagaattct agtattagaa gatggcacag tttttgaagg
    14161 caaggccttc ggagcagata ttgatgtaac aggcgaaatc gtctttaata cagggatgac
    14221 cggctaccaa gaatccatta cagaccagtc ttataatgga caaatcttga cctttactta
    14281 tcctttggta ggaaattatg gtattaatcg tgatgattat gaatccatta ttccaacttg
    14341 taagggagtc gttgtttttg aagaagcgcg tagagctagc aactggcgca accaaatgac
    14401 cttggatgaa tttttgaaag ccaagaaaat tccagggatt tcaggaattg atacgcgtgc
    14461 tcttaccaag attatccgta agcatggtac tatgcgtgca accttgaccc atgttgggga
    14521 cagtatggac catgtgacgg accagctcca agcaacagtt ttaccgacag acaatatcaa
    14581 acaggtttct actaaaacgt cttatccagc tccaggagtt ggtttgagcg tggtgctagt
    14641 ggactttggt ctcaagcact caatcctacg tgaactttct aaacgcaact gtaacgtgac
    14701 agttgttcct tattcgacaa cggctgaaga aattctccat ctcaatcctg acggagttat
    14761 gttgtcaaat ggtccaggta acccagaaga cgttccacaa gcactcgaca tgattcgcgg
    14821 tgtacaagga aaaattccaa tctttggtat ttgtatggga caccaactct ttgcaatggc
    14881 aaacggggct aagacctata agatgaagtt tggccaccgt ggattcaacc atgcggtacg
    14941 tgaaatcgca acaggacgag tagacttcac cagccagaac catggttatg cagtcagccg
    15001 cgaggatttg ccagagcact tgattattac ccacgaagaa atcaatgaca agtcagtgga
    15061 aggtgtgcgc cacagatacc aacctgcttt ctctgttcaa taccacccag atgcagctcc
    15121 tgggccacac gacgctagct acctatttga cgagtttatc gagatgatgg aagtttttaa
    15181 acaatcaaac taagaacgag taaaagctcg tcggagaatt tatgtaactg aacacggacg
    15241 gaaagctcag aatttagaga aaccaacttg gattgtcaat ccttccttga tttcttaaaa
    15301 ttcaatcgct ttctattcgt cctttgtatc ttatttaatg tcgccctgag aggcgacgaa
    15361 tagaaaggag aagggtgctc cgtatttgct gaactgaata cgggctacgg acggtgctaa
    15421 aaagatagtt attcctagaa ctgacagttc ttcgtcataa ctcctatttt agctttgtcc
    15481 gcttgacgcc cttaatatct tatatatgcc taaacgtact gatattcaaa aaattatggt
    15541 gattggttct ggtccgatta ttattggtca ggctgctgag tttgactacg ctgggaccca
    15601 ggcttgcttg tcgttgaaag aggaaggtta tgaggttgtt ttggttaact caaatcctgc
    15661 aaccatcatg acggacaagg aaattgctga caaggtttac attgaaccga ttacacttga
    15721 gtttgtgaca cgtattcttc gtaaggaagg tccagatgcc ttgctaccaa cactcggtgg
    15781 tcagacaggt ctcaacatgg ccatggaatt gtctaaaaat ggcatcttag atgagcttgg
    15841 tgtagagctt ctcggtacta aattgtctgc cattgaccaa gcggaggacc gtgacctctt
    15901 taaacaattg atggaagagc ttgagcaacc cattccagaa tctgaaattg ttaacacagt
    15961 agaagaagca gttgcctttg cagcgacaat tggctaccca gtcatcgttc gtccagcctt
    16021 tacccttggt ggtactggtg gtggtatgtg tgccaacgaa aaagaattgc gtgaaatcac
    16081 tgaaaatggt ttgaaattgt cacctgttac ccaatgtttg attgagcgtt cgattgccgg
    16141 tttcaaggaa attgaatacg aagtgatgcg tgactcagct gacaatgcct tggttgtttg
    16201 taacatggaa aactttgacc cagttgggat tcacacaggg gattccattg tatttgcccc
    16261 tgcgcaaacc atgtcagact atgaaaacca aatgcttcgt gatgcgagct tgagcattat
    16321 tcgcgccctc aagattgaag gtggatgtaa tgttcagcta gctcttgatc ctaacagctt
    16381 caagtattat gttatcgaag taaaccctcg tgtatcacgt tcgtcagccc ttgcttctaa
    16441 agcgacagga tatccgattg ctaagttggc tgccaagatt gcagtaggtt tgaccttgga
    16501 tgaggttata aacccagtta caggttcaac ctatgccatg tttgaaccag cccttgacta
    16561 cgtagttgct aagattccac gtttcccatt tgacaagttt gaaaagggtg aacgccgtct
    16621 cggtacacaa atgaaggcga ctggagaagt catggcaatc ggtcgtaaca tcgaggaatc
    16681 tcttctcaag gcctgccgct cccttgaaat tggggtgcac cacaatgaaa tacctgaact
    16741 tgcagcagtt tctgatgatg ccttgattga aaaggttgtc aaagcccaag atgaccgtct
    16801 cttctacgta tcagaagcca ttcgtcgtgg ctacacacct gaagaaattg cagagcttac
    16861 aaaaattgat atcttctatc ttgataaact cttgcatatc tttgaaattg agcaagaatt
    16921 gggtgctcat ccacaagatc tagaagtttt gaaaacagcc aaacttaatg gtttctcaga
    16981 ccgtaagatt gctgaactct ggggaacgac tgatgatcaa gttcgccaac ttcgcttgga
    17041 aaacaagatt gttccagttt ataagatggt agatacttgt gcggcagagt tcgactctga
    17101 aacgccatat ttctattcaa cctatggttg ggaaaatgaa tctatcagat ctgataaaga
    17161 atctgtactt gtcctaggtt caggtccaat ccgtatcggt caaggggttg agtttgacta
    17221 cgcaaccgtt cactctgtta aggctatcca ggcagctggt tatgaagcta tcatcatgaa
    17281 ctcaaaccca gagaccgttt caacagactt ctctgtatca gataagcttt actttgagcc
    17341 attgacattc gaagatgtta tgaacgtcat tgacttggaa caaccaaagg gagttatcgt
    17401 tcagtttggt ggtcaaacag ccatcaacct tgcggagcca ttggcaaaag caggtgtgac
    17461 catccttggt acacaggttg ctgacctaga ccgtgccgaa gaccgtgacc tcttcgagca
    17521 agctctcaaa gaattggata ttccacagcc accaggacaa acggctacca atgaagaaga
    17581 agcagctctt gcagctcgta agattggctt cccagttctt gtccgcccat cttatgtact
    17641 tggtggacgt gccatggaaa tcgttgaaaa cgaagaagac cttcgttctt acatgcgaac
    17701 tgctgttaag gctagtccag atcacccagt tcttgtcgac tcttatatcg ttgggcaaga
    17761 gtgcgaagtt gatgccattt cagatggtaa agatgtactc attcctggta tcatggagca
    17821 tatcgaacgt gctggtgtcc actcaggtga ctcaatggct gtttacccac cacaaacctt
    17881 gtcgcaaaag gttcaggaga ccatcgcaga ctacaccaaa cgcttagcaa tcggtcttca
    17941 ctgccttgga atgatgaaca tccagtttgt tatcaaggat gaaaaagtct acgttattga
    18001 ggtcaatcca cgtgccagcc gtactgttcc attcctatct aaggtaacca atattcctat
    18061 ggctcaagta gcgaccaagc tcattcttgg tcaaagttta tcagaacttg gctaccaaaa
    18121 tggactttac cctgaaagca cccgtgttca tatcaaggca cctgtcttct cctttaccaa
    18181 actagctaag gtagacagct tgctcggtcc tgaaatgaag tcaacaggtg aagttatggg
    18241 ttctgatgct actttggaaa aagctctcta taaagccttt gaagcttctt atctacactt
    18301 gccaactttt ggtaatgtcg tctttaccat cgctgatgat gccaaagaag aagccttgaa
    18361 cttggctcgt cgtttccaaa acattggtta tggaatcctt gcgacagaag ggacagcagc
    18421 cttctttgct agtcatggct tgcaagccca acctgttggt aagattggtg atgacgataa
    18481 ggatatccca agttttgtac gcaaaggaag aattcaagct atcattaaca cagtaggaac
    18541 aaaacgaact gctgacgaag atggtgagca aattcgtcgt tcagccattg aacacggggt
    18601 gcccctcttc acagcgctag atacagctaa tgccatgctt aaggtacttg aaagtcgtag
    18661 ctttgttaca gaagcgattt aaatagaaaa ataag
//
LOCUS       DAVKZB010000027        18347 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKZB010000027 DAVKZB010000000
VERSION     DAVKZB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.027"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(26..835)
                     /locus_tag="VXA80_000882"
     CDS             complement(26..835)
                     /locus_tag="VXA80_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0353406.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGIGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(837..1640)
                     /locus_tag="VXA80_000883"
     CDS             complement(837..1640)
                     /locus_tag="VXA80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0353407.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1650..3137)
                     /gene="tacF"
                     /locus_tag="VXA80_000884"
     CDS             complement(1650..3137)
                     /gene="tacF"
                     /locus_tag="VXA80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV0353408.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRAFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3493..4200
                     /locus_tag="VXA80_000885"
     CDS             3493..4200
                     /locus_tag="VXA80_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV0353409.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVLHAEDLVDKYLPLHKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIKLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4205..5227
                     /locus_tag="VXA80_000886"
     CDS             4205..5227
                     /locus_tag="VXA80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0353410.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMKVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5244..6113
                     /gene="licA"
                     /locus_tag="VXA80_000887"
     CDS             5244..6113
                     /gene="licA"
                     /locus_tag="VXA80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV0353411.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGTDEK"
     gene            6103..6981
                     /locus_tag="VXA80_000888"
     CDS             6103..6981
                     /locus_tag="VXA80_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV0353412.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            6993..7682
                     /locus_tag="VXA80_000889"
     CDS             6993..7682
                     /locus_tag="VXA80_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV0353413.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7778..8626
                     /gene="dprA"
                     /locus_tag="VXA80_000890"
     CDS             7778..8626
                     /gene="dprA"
                     /locus_tag="VXA80_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV0353414.1"
                     /translation="MKITNYEIYKLKKSSLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKTVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9510..10553
                     /locus_tag="VXA80_000891"
     CDS             9510..10553
                     /locus_tag="VXA80_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV0353415.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10720..12807
                     /gene="topA"
                     /locus_tag="VXA80_000892"
     CDS             10720..12807
                     /gene="topA"
                     /locus_tag="VXA80_000892"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV0353416.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFEPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKLKLTSNDEVKEILSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12913..13272
                     /locus_tag="VXA80_000893"
     CDS             12913..13272
                     /locus_tag="VXA80_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV0353417.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13365..13997
                     /locus_tag="VXA80_000894"
     CDS             13365..13997
                     /locus_tag="VXA80_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV0353418.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQLEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14041..14697
                     /locus_tag="VXA80_000895"
     CDS             14041..14697
                     /locus_tag="VXA80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV0353419.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14716..15810
                     /locus_tag="VXA80_000896"
     CDS             14716..15810
                     /locus_tag="VXA80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV0353420.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTLEEYGAIFKYLIENVKTERQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15822..16859
                     /gene="leuB"
                     /locus_tag="VXA80_000897"
                     /pseudo
     CDS             15822..16859
                     /gene="leuB"
                     /locus_tag="VXA80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            16856..17125
                     /locus_tag="VXA80_000898"
     CDS             16856..17125
                     /locus_tag="VXA80_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV0353421.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17129..17488
                     /locus_tag="VXA80_000899"
     CDS             17129..17488
                     /locus_tag="VXA80_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV0353422.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKFLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17650..18273
                     /locus_tag="VXA80_000900"
                     /pseudo
     CDS             17650..18273
                     /locus_tag="VXA80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5745 a         3157 c         3889 g         5556 t
ORIGIN      
        1 tagtagtttg tataatcccc ctcaatcagt ttttgcgata agctttaatg ctatgactat
       61 accactcttg catttctttt gatggtggtg tcatataatc gccatacatc tgggttaaaa
      121 attggtcata ttttttagga acaggcaaca tacggccctc aaactcagtt aaaatcagtt
      181 ctttaaaggt atcaactggg aagatttctt tcatcccttc cttaccgatc ccaattcctc
      241 cttcatattg aggagtgttg gttacagcat ttttgactag ttgatcaatt ttcttgtaaa
      301 agtagcgagg attgacaaat cggagagcgt accagctaca taatctaaga aaatctttta
      361 gtttgctatc accgtgaact gctcgtgatt ttttgatata agctagttga cgaagagcca
      421 catacttata gctcttgtcg acaatactca agtctgtaaa tcgatcaatt gggaagacat
      481 cgatgaaaag gctggtatca tgacgcttgt acttaacatg gtcttctata acagtagaag
      541 tgtccaaaat cgatgcgaaa ttatggaagt accaagaaga tgtatcgtag gaaagaacct
      601 tgtagcgagg gtgattttct tcttcaataa tcttcagtaa acgctcataa tcctcacgat
      661 aaagggaaat atcaatatca tcatcccaag gaatcatacc tttgtggcgg atggctccaa
      721 gcatggttcc ataactgaga aaataaggaa tatcatgttt cttacaagtc tcatcaatat
      781 agtccagcag ggctagttga atttctttaa tttctttttt ttctaaatat tgcatcctaa
      841 tcctccaatt tataagcgtg aaattcatga ctgtagaagc gtttttcttc tggtggtagg
      901 gtcatataat ctccataaaa ttgtgtcaaa atagtatcaa atttttcagg tgcaggaagg
      961 cttaaattct caaagggtaa atcgattgtt ttatcaaagg taccacttgg gaagacttcc
     1021 ttttccttaa attttgaagg aataaaagcc atatactgcc cattttcacg actatatttt
     1081 tgaatttctt tctcgatttt atttgcaaaa taacgaggag aaaccggtcg aaggagcaac
     1141 caaaaggctg ttcgtatcca atcttttaaa aggctatcct tatagacaat atttttatgt
     1201 ttactgaaag acagcagttt gaagctttcc agtttataac aagtatcaat gaccttagga
     1261 tcatcaaagc gatctatagg gaaaatatcg ataaagatac cagactcata ggtttttgta
     1321 tttcgagtat caataatttt agtcgtactg tcggttatct tgataaagtt gttaaagtag
     1381 ttcttatcag tttctaagga taggagctta tacttgcttt tttccttttg aaaaatgtta
     1441 ataaatcgtt ggtagtcttc tctaggcatg gataaatcaa tatcgtcgtc ccaagggata
     1501 aagccctcat gtcgaaccgc cccaatcaga gtaccgtagt taataatata gttgatattg
     1561 tgctttttac agagagtatc aatataatcc aaaatttcta attcaatttg tttggcatct
     1621 tcaacggtta gttgtttcat tttaaactcc tatgattttt taaatttatt ttttaaggct
     1681 aggacatggt ttaaaaattc atagaaaatg ctatcttttg tgaagacaag tagactaata
     1741 taagagatag ctgatagcaa gacaatcaaa ccagtattaa tcaaaaatgg caaattaatg
     1801 accatatcca caggatacat gaaattaatc aggaaataaa ttgctacaaa ggaaagtgaa
     1861 aagagagagt atcgaacagt atagctaaag atatgtccca agtggatgag ttgttttcta
     1921 tggatgaaaa tgatatagaa aacaagtaga gaagtctctg ataacatagt tgtcagtaag
     1981 tagtattcag gagccacgat atggttgaaa aagaggagac tattcaagcc caaatttagt
     2041 agcccagcaa agactgtata gactgtgata cgtttttcat agccattggt aaagagaatt
     2101 tgggaaccaa gaatggtatc taaggccaga ataatcgtac gaaaagcgaa gagagaggtc
     2161 aagatgccgc ctccgatata tttttcacta ccgtaaagta ggatggcatt tggtcctaaa
     2221 accatgagtc caaaactcag tggaatgata aagaagttaa agattcgact acctctatta
     2281 accagagaaa cataggcttc tttgtctcct ttccccagat agtaactgag acgaggcaca
     2341 ctcactccaa ttgcacctgt tacaacccca gctataacgg tcacaattcg ctgagctatg
     2401 gtatagtaac taacgttgac atcaatccct gttttaacga ggaagaggcg atctaaaaaa
     2461 gtgaagagca tattggcatt ggcaaagact aacatggctg tcagagggag aaagagtggt
     2521 ttaaaatcac ttaggtgaat cttaacaagt ttgatgtctc ttttaatcca aaaataacta
     2581 atcaggtagt taatcagcgt cgataaactc atcacaagtg tatagacaac aatatcgtgt
     2641 tcatttttaa caaataagaa aatagagacc agcatcagga tacggatgaa ggcagttttg
     2701 taaaagagaa aactgtaatt ttccagagct tcattgaccc attcgattga aaaaatctgg
     2761 gcaatgagtt gaatccccat aacaaggtag acctttttga cgattggatt atcagtaaag
     2821 aagagaggat aggctaggat atagacagca gtggtcaaaa tcgtacaagc gatgcacaaa
     2881 taaaaaagac tagaaaaggc tctgttaaga tcttttttgt tatccttgac attactgata
     2941 gcccttaaac cgtagttata gacaccataa gttgcaaagg gcaagaaaaa tgacaaaata
     3001 gtgtcgactg agttgaagta accatagtca gttcggtcca agacacgcgc gacataggtt
     3061 ccagttagga tgggaaaaat aatattcaag acacgaattc ccatgtaaga tagagcattt
     3121 aattttatac ttttcattca atttacctcg tttttcatta tatcataaag ttagctaata
     3181 agaaatgaag ggcagtaagt caagtaatca ctttgaagtt tcaaatctta aattttaagt
     3241 tttctttaag gaaagtatat tattctgaaa gactctaaaa tttcgcagtc atttattagt
     3301 aattgctaca gaattcctag tcattactag aaataggctt gtttctttga ataatagaac
     3361 tgcataattc tcctattcta gaaggggagg accagtattt ctttcatgat aggactagat
     3421 tgtggtatat aggactagat tgtggtataa tagagagaat aagttttttt agtaagacaa
     3481 aggagaaaat agatgattta tgcaggaatt cttgccggtg gaactggcac acgcatggga
     3541 atcagtaact tgccaaaaca atttttagag ctaggtgatc gacctatttt gattcataca
     3601 attgaaaaat ttgtcttgga accaagtatt gaaaaaattg tagttggtgt tcatggagac
     3661 tgggttttac atgcagaaga tcttgtagat aaatatcttc ctcttcataa ggaacgtatt
     3721 atcattacaa agggtggtgc tgaccgcaat acaagtattg agaacatcat tgaagccatt
     3781 gatgcttatc gcccgcttac tccagaggat atcgttgtta cccacgattc tgttcgtcca
     3841 tttattacgc ttcgcatgat tcaagacagt atcaaacttg ctcaaaatca tgacgcagtg
     3901 gatacagtag tagaagcagt ggatactatc gttgaaagta ccaatggtca attcattaca
     3961 ggtattccaa atcgtgctca cctctatcag ggacaaacac ctcaaacatt ccgttgcaag
     4021 gacttcatgg acctttatgg atctctttct gatgaagaga aggaaatctt gacagatgca
     4081 tgtaaaatct ttgtgatcaa aggaaaagat gtagccttgg ccaaaggcga atactcaaat
     4141 ctgaagatta caaccgtaac agatttgaag attgcaaaaa gtatgattga gaaagactag
     4201 taaaatgatt aatcaaattt atcaactaac taaacctaag tttatcaatg tcaaatatca
     4261 ggaagaggct attgaccaag agaatcatat ccttatccgt cccaactaca tggctgtctg
     4321 tcatgcggat cagcgttact accagggaaa acgtgatccc aagattttga ataaaaagct
     4381 tccaatggca atgattcacg agtcatgtgg aaccgtcatt tctgacccga caggaaccta
     4441 cgaggttggt caaaaagttg tcatgattcc caatcagtct cctatgcaga gtgatgaaga
     4501 attctatgaa aactacatga cagggaccca tttcttgtct agtggatttg atggctttat
     4561 gagagagttt gtttctctcc ctaaagatcg tgtggtggct tatgatgcta ttgaagatac
     4621 ggttgcagcc attacagagt ttgtcagtgt gggcatgcac gctatgaatc gtctattgac
     4681 tcttgctcat agcaagcggg agcggatcgc cgttattgga gatggaagtt tagcttttgt
     4741 ggttgccaat attatcaatt atactttgcc agaagcagag attgtggtta ttggtcgtca
     4801 ttgggaaaag ttggaactct tctcatttgc caaagaatgc tatattacgg ataatattcc
     4861 tgaagagttg gcctttgacc atgcttttga atgttgtggt ggtgatggta ctggaccagc
     4921 tattaatgac ttgattcgct acattcgtcc tcagggaaca attctcatga tgggagttag
     4981 cgaatataaa gtcaatctca atactcgcga tgccttagaa aagggcttgc tcttggttgg
     5041 gtcatctcgt tctggtcgca ttgattttga aaatgctatc caaatgatga aagtcaagaa
     5101 atttgccaat cgtcttaaaa atatccttta tctagaagaa cctgtaagag aaattaaaga
     5161 tattcaccgt gtctttgcaa ccgatttaaa tacagccttt aaaacagtgt ttaagtggga
     5221 agtataagtc ctggaggtta attgtggaga aaatcattaa agaaaaaatt tcttccttac
     5281 ttagtcaaga agaagaagtc ctcagtgttg aacaactggg tggaatgacc aatcaaaact
     5341 atttggccaa aacaacaaat aagcaataca ttgttaaatt ctttggtaaa gggacagaaa
     5401 agcttatcaa tcgacaagat gaaaagtaca atcttgaact actaaaggat ttaggcttag
     5461 atgtaaaaaa ttatcttttt gatattgaag ctggcatcaa agtaaatgag tatatcgaat
     5521 ctgcgattac gcttgattca acgtcaatca agaccaagtt cgacaaaatt gctccaatat
     5581 tacaaactat tcatacatct gctaaggaat taagaggaga atttgctcct tttgaagaaa
     5641 tcaaaaaata cgaatccttg attgaagaac aaattcctta tgccaactat gaatctgtta
     5701 gaaatgcagt cttctcctta gagaaaagac tggctgactt aggtgttgac agaaaatctt
     5761 gtcatatcga tttggtgcct gaaaacttta tcgaatcacc tcaaggacga ctttatttga
     5821 ttgactggga atattcatca atgaatgatc caatgtggga tttggctgcc ctctttttag
     5881 agtctgaatt cacttcccaa gaggaagaaa ctttcttatc tcactatgag agtgaccaaa
     5941 caccggtttc tcatgaaaag attgctattt ataaaatttt acaagatact atttggagtc
     6001 tatggactgt ctataaggaa gagcaaggtg cagattttgg ttactatggt gtgaatcgtt
     6061 accaaagagc tgttaaaggt ttggcttctt atggaggaac agatgaaaag taaaaacgga
     6121 gttccttttg gccttctctc aggtattttc tggggcttgg gtctaacggt tagtgcttat
     6181 atcttttcga tttttacaga tttgtcaccc tttgtggtgg ctgcagctca tgattttttg
     6241 agcatcttta tcttactagc ttttctcttg gtaaaagaag ggaaagttcg cctctcaatt
     6301 ttcttaaata ttcgcaatgt cagtgttatc atcggagcct tgctagcagg ccctatcggt
     6361 atgcaggcca atctttatgc agttaagtat atcggaagtt ctttagcttc atctgtatcg
     6421 gctatttacc ctgcgatttc agttctattg gctttcttct ttttgaagca caagatttcg
     6481 aaaaatactg tatttgggat tgtcttgatt attggaggga ttattgccca gacctataag
     6541 gttgaacagg ttaattcttt ctacattggg attctttgtg ctttggtttg tgctattgca
     6601 tggggaagtg agagtgttct tagctctttt gccatggaaa gtgaattgag tgaaatcgaa
     6661 gccctcttaa tccgtcaagt aacttcgttc ttgtcctatc ttgtgattgt gctcttctct
     6721 catcagtcat ttgttgcagt agccaatgga caattgctag gtctcatgat tgtctttgta
     6781 gcctttgata tgatttccta tttggcttat tatatcgcta tcaatcgctt gcaaccagcc
     6841 aaggctacag gcttgaacgt gagctatgta gtatggacgg tcttgtttgc agttgttttt
     6901 ttgggtgcac cgctagatat gctgaccatt atgacgtcac ttgtcgtcat tgctggagtt
     6961 tatattatta ttaaagaata aaggagattc gtgtgaaagc cattatctta gcagcgggat
     7021 tgggaactcg cttgcgtcct atgactgaaa atacccctaa agccttggtt caggttaatc
     7081 aaaaaccttt gattgaatac caaattgagt ttctcaaaga aaaaggaatc aatgacatca
     7141 tcatcattgt tggttatctt aaagaacaat tcgattactt aaaagaaaaa tacggtgttc
     7201 gcctcgtttt caatgataaa tacgctgact acaataactt ttactctctc tatcttgtaa
     7261 aagaagaatt ggccaacagc tatgttattg atgctgacaa ttatctcttt aaaaatatgt
     7321 tccgcaatga tttgactcgt tcgacttatt ttagtgttta tcgtgaagat tgtaccaacg
     7381 aatggttctt ggtctatgga gatgactaca aggttcaaga cattattgtt gatagcaagg
     7441 caggtcgcat ccttagtggt gtatccttct gggatgctcc aactgcagaa aagattgtca
     7501 gctttatcga taaggcttat gcaagtggcg aatttgttga tctctattgg gacaatatgg
     7561 ttaaggataa tatcaaagag ctagatgtct atgttgaaga attagaaggc aatagcattt
     7621 atgagatcga tagtgtccaa gactatcgta aattagaaga aattcttaaa aacgaaaatt
     7681 aaagattcca acatctgaca aaatagtcgg atgttttttg attttttacg aacttttacg
     7741 aatagataga tgagtagaaa aagaaatgga gttatttatg aaaatcacaa actatgaaat
     7801 ctataagtta aaaaaatcaa gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg
     7861 tgaaaatgtt gatcaggagc ttttgttggg tgatattgca gatatatcag gttgccgtaa
     7921 tccagccgtt tttatggaac gttattttca gatagacgat gcgcatttgt cgaaagagtt
     7981 tcaaaaattt ccatctttct ctattttaga tgactgttat ccttgggatt tgagtgaaat
     8041 atatgatgcg cctgtacttt tattttacaa gggaaatctt gacctcctga aattcccgaa
     8101 ggtagcggtc gtgggcagtc gtgcttgtag caaacaggga gctaagacag ttgaaaaagt
     8161 cattcaaggc ttggaaaatg aactggttat tgtcagtggt ctggccaagg gcattgacac
     8221 agcagctcat atggcagctc ttcagaatgg cggaaaaacc attgcagtga ttggaacagg
     8281 actggatgtg ttttatccta aagccaataa acgcttgcaa gactacatcg gcaatgacca
     8341 tctggttcta agtgaatatg gacctggcga acaacctctg aaatttcatt ttcctgcccg
     8401 taatcgcatc attgctggac tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc
     8461 aggtagtctc attacctgtg agcgagcaat ggaagaagga cgcgatgtct ttgctattcc
     8521 tggtagcatt ttagatggac tatcagacgg ttgccatcat ttgattcaag aaggagcaaa
     8581 attggtcacc agtgggcaag atgttcttgc ggaatttgaa ttttaaaaat gacctaagct
     8641 agaattctaa gaaaaaatca attttaagag aaaatgaacc caacatttcc ataataaaac
     8701 gcatattagc aagtttttaa cacttgataa tatgcgtttt ttctaagtgg attagtagag
     8761 tagaggattt ttctcatata atactcttcg aaaatctctt caaactacgt cagcttccat
     8821 ctgcaacctc aaaacagtat tttgagctga cttcgtcagt cttatctaca acctcaaagc
     8881 agtgctttga gcaacctgtg gctagcttcc tagtttgcgc tttgattttc attgagtata
     8941 agggaaagta tagtgaattg aaataagatg tgaacaactc tatcaggaaa gtcaaattaa
     9001 tttatagaaa tattttagca gccaaggtgc actgttatag attcaataca ctataattta
     9061 gtgtaattga gaaaggagaa atgattgtga ttgatgttgg ctaggttatg ttcaatgatt
     9121 cctaccgtct caaatcttgt cagtaaggaa aaataaattc ttcaaaagta gagattacaa
     9181 ggcttgttta agaaagaatt caaagacctt gacaaataaa aataaaatgg ttattataag
     9241 aaatggtcta aaatagatga tgatactttt cgaaaatctt tttacgtcag ctcagctttg
     9301 ccttgctgtg ttttgagcaa gctacggtta gcttccgagt ttgattttca tttactagaa
     9361 atgaaactga tgagagatat cagtagacat ttgagtcagg atattatgga aaatgataaa
     9421 aagagctcgt gagattggca tatcagacta ctaaagtatt gagtttgtta ggattttagc
     9481 gactagttag ctgggaaagg aagatatttg tgacaaataa taaactgtat tcgttgatag
     9541 aatttagaaa taaaatatat gaagaattag aactttccag aagtgattta gcgattttac
     9601 tatgtgccat gcttatcgcc tctatcggat taaatatgga ttcgactccc gtgattattg
     9661 gagccatgtt aatctctcct ttgatgacac ctattctggg agtggggctc tctctagcta
     9721 tatttgattt taaattgtta agaaaatctt ttaaaatatt agctattcaa attcttgcga
     9781 gtctaatagc ttcaacactt tatttttatc tttctcccat ttcgtatgct agttcggaga
     9841 ttgttgctag aacctctccg actatttggg atgttctcat tgcttttgta ggagggatag
     9901 caggtattat tggtgctagg aaaaaagaga ccaataatat tgttcctggt gttgctattg
     9961 caaccgcctt gatgcctcct ctttgtacag taggttatgc tattgcttct gctaatctaa
    10021 aatttatcat aggctcctct tacctattcc tcatcaattg tagctttatt gtcattgcga
    10081 cttatatagg tgttaggttg atgatggtta agaaacatta ttttaaagat aatgaagaag
    10141 actctaaaat gcgtaggatt ttgcttctag ttgctgtttt gctgatgatt ccgagtttca
    10201 tctctgcaac gactttagtg agagaaacgt tgaaaaaaga gtcccttaag aaatttatat
    10261 cagagcagtt tcaggggtat aatattttga aaaaaaccta ttctaaaaag actcataccc
    10321 taaagctaac catttcagga aattatttga cagaagaaga actcgatatg atttcaagta
    10381 agagaggtga ctatggttta agtgatgttt ctgttcaagt ttcacaattg tctgattcag
    10441 aacaacttag taaggaagag ctggtggagt atttcttcca gtatatcaag gataaggaag
    10501 caaaagaaaa ggagaaagcc aataaatttg atacagagtc tgaggagcaa taatttcttg
    10561 agaatagctg gtttttctcg tgagtcttct atgtatatca aaggaagact gaggttttaa
    10621 gtataaaact tttcttctat tatagtagct aagtcttgac agggttcaaa aaatggttta
    10681 cactttataa agtttattac tttgaaaagg tgtgatactg tggctacggc aacaaaaaag
    10741 aaaaaatcaa cagttaaaaa aaatctagtc atcgtggagt cgcctgctaa ggccaagacg
    10801 attgaaaaat atctaggcag aaactacaag gttttagcca gtgtcgggca tatccgtgat
    10861 ttgaagaaat ccagtatgtc cgtcgatatt gaaaataatt atgaaccgca atatattaat
    10921 atccgaggaa aaggccctct tatcaatgac ttgaaaaaag aagctaaaaa agctaataaa
    10981 gtttttctcg cgagtgaccc ggaccgtgaa ggagaagcga tttcttggca tttggcccat
    11041 attctcaact tggatgaaaa tgatgccaac cgtgtggtct tcaatgaaat caccaaggat
    11101 gcagtcaaaa atgcttttaa agaacctcgt aagatcgata tggacttggt cgatgcccaa
    11161 caagctcgtc ggatcttgga tcgcttggta gggtattcga tttcgcctat tttgtggaag
    11221 aaggtcaaga agggcttgtc agcaggtcgc gttcagtcca ttgcccttaa actcatcatt
    11281 gaccgtgaaa atgaaatcaa tgccttcgag ccagaagaat actggacagt tgatgctgtc
    11341 tttaaaaagg gaaccaaaca atttcatgct tccttctatg gagtagatgg taaaaagctg
    11401 aaactgacta gcaatgacga ggtcaaggaa atcttgtctc gtctgacgag taaagacttt
    11461 tcagtggatc aggtggataa aaaagagcgc aagcgcaatg ctcctttacc ctataccact
    11521 tcatctatgc agatggatgc tgccaataaa atcaatttcc gtactcgaaa aaccatgatg
    11581 gttgcccaac agctctatga aggaattaat attggttctg gtgttcaagg tttgattacc
    11641 tatatgcgta ccgattcgac tcgtatcagt cctgtagcgc aaaatgaggc ggcaagcttc
    11701 attacggatc gttttggtag caagtattct aagcacggta gcaaggtcaa aaacgcatca
    11761 ggtgctcagg atgcccatga ggctattcgt ccgtcaagtg tctttaatac accagaaagc
    11821 atcgctaagt atctggacaa ggatcagctc aagctatata cccttatctg gaatcgtttt
    11881 gtggctagcc agatgacagc ggccgttttt gataccatgg ctgttaaatt gtctcaaaat
    11941 ggagttcaat ttgctgccaa tggtagtcag gttaagtttg atggttatct tgccatttat
    12001 aatgattctg acaagaataa gatgttaccg gacatggttg ttggagatgt ggtcaaacag
    12061 gtcaatagca aaccagagca acatttcacc caaccgcctg cccgttattc tgaagcaaca
    12121 ctgattaaaa ccttagagga aaatggggtt ggacgtccat caacctatgc gccaaccatt
    12181 gaaaccattc agaaacgtta ttatgttcgc ctggcagcca aacgttttga accgacagag
    12241 ttgggagaaa ttgtcaataa gctcatcgtt gaatatttcc cagatatcgt aaacgtgacc
    12301 ttcacagctg aaatggaagg taaactggat gatgtcgaag ttggaaaaga gcagtggcga
    12361 cgggtcattg atgcctttta caaaccattc tctaaagaag ttgccaaggc tgaagaagaa
    12421 atggaaaaaa tccagattaa ggatgaacca gctggatttg actgtgaagt gtgtggcagt
    12481 ccaatggtca ttaaacttgg tcgttttggt aaattctacg cttgtagcaa tttcccagat
    12541 tgccgtcata cccaagcaat cgtgaaagag attggtgttg agtgtccaag ctgtcatcag
    12601 ggacaaatta ttgagcgaaa aaccaagcgt aatcgcctct tctatggttg caatcgctat
    12661 ccagaatgtg aatttacctc ttgggacaag cctgttggtc gtgactgtcc aaaatgtggc
    12721 aacttcctca tggagaaaaa agtccgtggt ggtggcaagc aggttgtttg tagcaaaggc
    12781 gactacgagg aagaaaagat taaataagag gaggggcccg aaagtaaatt tcaggctcct
    12841 tttggttaga acttgacaaa atacatcatt ttatgcgaaa ctagaagaag attattttaa
    12901 ctaggagaag ttatgcgtct tatctatctg ataattggtt ttttatcact ggccttggct
    12961 attgttgggg ttgttttacc cttattgccg acaacgcctt tccttttgtt gtctattgct
    13021 tgtttctcca gaagttccaa gcgtttcgaa gactggcttt atcataccaa gctctatcaa
    13081 acatatgtag ctgattttcg cgagactaag tctattacgc gtgaacgaaa gaaaaaaatc
    13141 atcgtctcta tctacgtctt gatgggaatt tctatttatt ttgcacctct tttaccagtc
    13201 aaaattggtc tgggtgcttt gaccatcttt atcacttatt atctcttcaa ggtcattcca
    13261 gacaaagaat agttaaaaca gtagttattt gccttgataa aattgaaagc atattcataa
    13321 caatatgata taataaattt gaagtaatat tcaaggagaa tcaaatgatt tacgaatttt
    13381 gtgctgaaaa tgtgacttta cttgaaaaag cgatgcaggc tggagctcgt cggattgaac
    13441 tctgtgataa tctagcagtt ggtgggacaa cacccagcta tggagtgact aaggcagcgg
    13501 ttgaactggc agctaactac gatacaacca tcatgaccat gattcggcca cgtggtggtg
    13561 actttgtcta taatgaccta gaaattgcta tcatgctaga agacattcgt ttgactgctc
    13621 aggctggaag tcaaggggtt gtatttggaa ctttaactgc tgataaaaag ttggataagc
    13681 ctaatctgga aaagttaatt gctgcatcaa aaggaatgga aattgtcttt cacatggcct
    13741 ttgatgaact aagtgatgaa gatcaactgg aagctattga ctggctcagt caagccggtg
    13801 tcactcgtat cctaactcgt gctggtgtgt ctggcgactc cttagaaaaa cgttttgttc
    13861 actatcacag aattttggag tacgctaaag gtaaaattga aattctacca ggtgggggga
    13921 ttgaccttga aaaccgtcaa acctttatcg accaggtggg ggtaacacaa ttgcatggta
    13981 ctaaggttgt tttttaaaaa atagaaagga actgctagcc ttgggtagca gttttcactt
    14041 atgtttgaaa tttttaaatc ctatcaattt aatcaagaaa aggctcatga ttatggtttt
    14101 atagaaaata gcgaagtctg gacatatagt tgccagattt tgcaaggtga ctttgtcatg
    14161 actgtgtcca tcactgctga taatgtgaac tttcaagtct ttgaccaaga gactggtgac
    14221 ctctatcctc acgttcatat ggaaagcatg aggggaagtt ttgtcggaaa tgtccgtgag
    14281 gcttgtctgg agattcttta ccagattcgg aaggcttgtt ttgatgtgca agattttatc
    14341 tgtcatcaga ctaagcgtat catgactcaa gttcaggaaa agtatggaaa ccagttggag
    14401 tatctgtggg aaaaatcgcc tgatacagct gtattgcgcc atgaaggcaa tcaaaagtgg
    14461 tatgccgtct tgatgaaaat ctcttggaat aagctggaaa agggcagaga aggacaagtg
    14521 gaagcagtca acctcaagca tgaccaagta gctaatttac tttcacaaaa ggggatttat
    14581 ccagcctttc atatgagcaa gcgctactgg attagtgtgt cccttgatga tactttatca
    14641 gatgaagaag tactggaatt gatagaaaaa agttggaact taacctctaa aaaatgaaat
    14701 attttaataa ttttcatgaa ctttcaatta gctaaatatt ctttactgaa gaaattttca
    14761 gaaaatatag gatttaccac actagaggaa tatggtgcca tcttcaaata cctgattgag
    14821 aatgtcaaga cggagcgtca gatcatttat tcgcctcact gtcatgatga cctcggaatg
    14881 gcagtggcaa atagccttgc tgctgtcaag aatggtgcag gacgtgttga agggactatc
    14941 aatggtatta gggagcgagc tggaaatgct gctttggaag aaattgcagt ggctctcaat
    15001 attcgccaag attactacca agtagaaacc agtattgtcc taaatgagac catcaatacg
    15061 tcagaaatgg tttctcgctt ctctggtatt ccagttccta aaaacaaagc cgtcgttggt
    15121 ggcaatacct tctcccacga atctggtatt caccaagatg gagtccttaa aaatcctctc
    15181 acttatgaga tcatcacacc tgaattggtt ggtgttaaga ttctgcttgg aaaattatct
    15241 ggtcgccatg cttttgttga gaaactgaga gaattggccc tagattttac agaagaggat
    15301 atcaaaccac tctttgctaa gttcaaggca ctggtcgata agaagcaaga aatcacagat
    15361 gcagatattc gagctttggt agctggaacc atggttgaaa atccagaagg cttccacttt
    15421 gatgatttac aacttcaaac tcatgcagat aatgacattg aagcgctcgt tagcctagcc
    15481 aatatggatg gtgagaaagt cgaatttaat gcgacagggc aaggttccgt tgaagcaatc
    15541 tttaatgcta tcgataagtt ctttaaccaa tctgttcgtt tggtgtccta cactatcgat
    15601 gcggtaacag atggaatcga tacccaggat cgggttttgg tcactgttga aaacagagat
    15661 acagaaacca tctttaatgc agcagggctt gattttgatg tgttgaaggc ttctgctatt
    15721 gtctatataa acgctaatac ctttgttcaa aaagagaatg caggtgagat gggacgcagt
    15781 gtttcttacc acgatatgcc tagtgtgtaa aggagaaggc tatggcaaag aaaatagtag
    15841 ctctagcagg agacggaatt ggcccagaaa tcatggaggt tggtttagaa gttctggagg
    15901 ctctagctga aaaaacaggt tttgactatg agattgacag acgaccgttc ggaggtgcag
    15961 atattgatgc agcatgacct cccttacctg atgaaaccct taaggcaagt agggaagcag
    16021 atgctatcct actagcagct atcggtagtc ctcagtatga tggagcagtg gttcgccctg
    16081 aacaaggcct gatggctctc cgtaaggaac tcaatcttta cgctaatatt cgtcctgtaa
    16141 aaatctttga cagtctcaag catttgtcac tactcaaact ggaacgaatt gctggtgtag
    16201 actttgtcgt ggtgcgtgaa ttgacaggcg ggatttactt tggagatcat attcttgaag
    16261 agcgcaatgc gcgtgatatc aacaactata gctatgagga agtggagcgg attattcgca
    16321 aagcctttga aattgcaaga aatcgcagaa aaatcgttac tagtatcgat aagcaaaatg
    16381 ttctagcgac ctcaaaactc tggcggaaag tagctgagga agtcgcacag gatttcccag
    16441 atgtaacctt ggaacatcag ctggtagact cagctgctat gcttatgatt accaatcctg
    16501 ctaagtttga tgttattgta acggagaatc tttttggaga tattttatct gatgaatcaa
    16561 gcgtcttatc tggtacactt ggggttatgc catcagccag tcattctgaa aatggaccaa
    16621 gtctctatga acctattcac ggttcagcac ctgatattgc aggtcaagga attgccaatc
    16681 ctatttccat gattttatca gtttccatga tgttgagaga tagtttcgga cgttatgagg
    16741 atgcagagcg tatcaaacgt gctgttgaga caagtctggc ggcaggaatt ttaacgagag
    16801 atataggagg tcaggcttca acaaaggaaa tgacggaagc tattattgca aggttatgaa
    16861 gttagacgaa aaaattactc tagtcctttt gatttggaat gtcatcattt tcttgattta
    16921 tggcattgac aaattcaagg caagaagaag agcttggcgc atccaagaga aaatcttact
    16981 cattttagcc cttacttgtg gtggttttgg ggcctggtta gctggaatca cttttcacca
    17041 caagactcga aaatggtact ttaaaacagt ttggtttctt gggatggtga ccacactagt
    17101 agccttatat tttatttgga ggtaatggat ggcagggtct tcgagggaat acgctgcttg
    17161 ggctctagcg gactatggtt ttaaggtcgt gattgcagga tctttcggtg acattcatta
    17221 caataatgaa ctcaataatg gcctgttgcc aatcgttcag cctagagagg ttagagagaa
    17281 actagcccag ctaaaaccaa ccgaccaggt aactgtggac ttggaacaac aaaaaatcat
    17341 ctcaccagtt gaagaattca ccttcgagat agatagcgag tggaaacata aattcctaaa
    17401 tagtttggat gatatcggta ttaccttgca gtatgaagag ttgattgctg cttatgaaaa
    17461 acaacgacca gcctactggc aggattagaa aaaatagaaa aggaaatata gtaaactgaa
    17521 ataagatgta aacaaatgaa ttggagctta acatccattt ccagcaattt tttagaaact
    17581 acagtggact attctggatt caacacatta taaaattatg acaaaacaca ttcacaagaa
    17641 ggctacgaca ttttaaaagg tgagggcgga tgtatcgttt gccctactaa agttggttac
    17701 attatcatga ccagcaacaa ggcaggactt gagcgtaagt tcgcagccaa agaacgtaag
    17761 cgtaacaaac caggtgttgt tctctgcggt agcatggatg aacttcgcgc tttagcgcaa
    17821 ctcaacccag aaattgaagc attctactaa aaacattgga tgaagatatt cttcttggtt
    17881 gtatccttcc ttggaaacca gaagcctttg aaaaactcaa agcatacggg gatggccgtg
    17941 aagaacttat gactgatgta cgtggtacta gctgttttgt tatcaagttt ggaaaagcag
    18001 gtgaacaatt ggctgccaag ctttgggaag aaggtaaaat ggtctacgcc tcatatgctt
    18061 caatgacaaa acgattgaaa ctcgctatga gagcaaggtg taatggtgtc tatggtcgat
    18121 aaggacggca aactcatccc agaacaagga ggagcacgtt caacttcacc agcaccagtt
    18181 gtgatccgta aagggcttga cattgataaa atcatgatgc acctgtcaga tacttttaac
    18241 tcatgggact accgtcaggt tgagtattat taagataaaa aaagaagtct agtgttatga
    18301 gatattaaag ctcctaacac tggacttttt gtttagaatt tcttttc
//
LOCUS       DAVKZB010000028        18298 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKZB010000028 DAVKZB010000000
VERSION     DAVKZB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.028"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..249
                     /locus_tag="VXA80_000901"
     CDS             <1..249
                     /locus_tag="VXA80_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV0353423.1"
                     /translation="QYMKEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYG
                     LIYVDRNDDGNGTFNRYKKMSFTWYKGVIESNGESLFK"
     gene            732..1778
                     /gene="pheS"
                     /locus_tag="VXA80_000902"
     CDS             732..1778
                     /gene="pheS"
                     /locus_tag="VXA80_000902"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV0353424.1"
                     /translation="MSTIEEQLKALREKTLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1778..2287
                     /locus_tag="VXA80_000903"
     CDS             1778..2287
                     /locus_tag="VXA80_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353425.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            2364..4769
                     /locus_tag="VXA80_000904"
     CDS             2364..4769
                     /locus_tag="VXA80_000904"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV0353426.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNESAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPCRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQLSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEATLQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4837..5838)
                     /locus_tag="VXA80_000905"
     CDS             complement(4837..5838)
                     /locus_tag="VXA80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0353427.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5994..6397
                     /locus_tag="VXA80_000906"
                     /pseudo
     CDS             5994..6397
                     /locus_tag="VXA80_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6523..6789
                     /locus_tag="VXA80_000907"
     CDS             6523..6789
                     /locus_tag="VXA80_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0353428.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            7050..9299
                     /gene="metE"
                     /locus_tag="VXA80_000908"
     CDS             7050..9299
                     /gene="metE"
                     /locus_tag="VXA80_000908"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV0353429.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGASRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDTDYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9363..10229
                     /gene="metF"
                     /locus_tag="VXA80_000909"
     CDS             9363..10229
                     /gene="metF"
                     /locus_tag="VXA80_000909"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV0353430.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10914..13127
                     /gene="pnp"
                     /locus_tag="VXA80_000910"
     CDS             10914..13127
                     /gene="pnp"
                     /locus_tag="VXA80_000910"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV0353431.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            13143..13760
                     /gene="cysE"
                     /locus_tag="VXA80_000911"
     CDS             13143..13760
                     /gene="cysE"
                     /locus_tag="VXA80_000911"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV0353432.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRVHGKKDEPVIHEVEEKREYYVNKLEQAKEASHRSSGL"
     gene            13772..14656
                     /locus_tag="VXA80_000912"
     CDS             13772..14656
                     /locus_tag="VXA80_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353433.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14738..16081
                     /locus_tag="VXA80_000913"
     CDS             14738..16081
                     /locus_tag="VXA80_000913"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV0353434.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            16074..16460
                     /locus_tag="VXA80_000914"
     CDS             16074..16460
                     /locus_tag="VXA80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV0353435.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16464..17348
                     /locus_tag="VXA80_000915"
     CDS             16464..17348
                     /locus_tag="VXA80_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV0353436.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17485..17940
                     /locus_tag="VXA80_000916"
     CDS             17485..17940
                     /locus_tag="VXA80_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353437.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            17937..18083
                     /locus_tag="VXA80_000917"
     CDS             17937..18083
                     /locus_tag="VXA80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353438.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
BASE COUNT         5520 a         3493 c         4127 g         5158 t
ORIGIN      
        1 cagtatatga aagaacattt attgcaagta gctgaagctc tacaagatgg tgtggaaatt
       61 atgggctata cgtcttgggg atgtattgac tgcgtttcta tgtccactgc acagctttct
      121 aagaggtacg gtctcattta tgttgatcga aatgatgatg gaaatggcac attcaatcga
      181 tataagaaaa tgtcctttac ttggtataaa ggagtgattg aatcaaatgg agaatcctta
      241 ttcaaatagt ttgtttattg atgattagat attatcacgg ttaaggcaac atctcattga
      301 atatggaata aagaagtttg gaagacatta aattctaaac ttctttattt tataggggag
      361 tgtcaattaa tttaattttg ttacaaaaat tataaatagt taatttagat aaattgttta
      421 tataaattta gataagaata agctaacaag cagaagtaag ataggtcttg caagaatggt
      481 aaaaatctga taaactaagt agtaattgaa taggaatctg aaagactagt agcccaaggg
      541 aagtatatca gggaggagag ccgtgactgc aagctctcta tatgaaagct gggtgaattc
      601 acttgcgcat ggatttagaa atgaaggtct gacttgtcag atgaaaacgg atggtaccgc
      661 gtgtcaacgc tccgagtgga gtttttggca tgtggttttc tttttatcta cgagagactg
      721 atggaggaaa tatgtcaact attgaagaac aattaaaagc gcttcgcgaa aaaacgctga
      781 ctagcttgaa gcagattact gctggaaatg aaaaagagat gcaagatttg cgtgtctctg
      841 tccttggtaa aaagggttcg ctcactgaaa tcctcaaagg gatgaaagat gtttctgctg
      901 agatgcgtcc aatcatcggg aaacacgtca atgaagctcg tgatgtcttg actgctgctt
      961 ttgaagaaac agctaagctc ttggaagaaa agaaagtcgc ggctcaactg gctagcgaga
     1021 gtatcgatgt gacgcttcca ggtcgtccag ttgcgactgg tcaccgtcac gttttgacac
     1081 aaaccagtga agaaatcgaa gatatcttca tcggtatggg ttatcaagtc gtggatggtt
     1141 ttgaagtgga gcaagactac tataactttg aacgtatgaa ccttccaaaa gaccacccag
     1201 ctcgtgatat gcaggatact ttctatatca ctgaagaaat cttgctccgt acccacacgt
     1261 ctccagttca ggcgcgtgct atggatgccc atgatttttc taaaggtcct ttgaagatga
     1321 tctcgccagg gcgtgtcttc cgtcgcgata cggacgatgc gacccacagt caccaattcc
     1381 accaaatcga aggcttggta gttgggaaaa atatctctat ggctgatctt caaggaacgc
     1441 ttcagttgat tgtccaaaaa atgtttggtg aagagcgtca aattcgtttg cgtccatctt
     1501 acttcccatt cacagagcca tctgttgagg tggatgtttc ttgcttcaag tgtggtggag
     1561 aaggctgtaa tgtatgtaag aaaacaggtt ggatcgaaat tatgggggcc ggtatggttc
     1621 acccacgtgt ccttgaaatg agtggtatcg atgcgactgt atactctggc tttgcctttg
     1681 gtcttggaca agagcgtgta gctatgctcc gttatggaat caacgatatc cgtggattct
     1741 accaaggaga tgtccgcttc tcagaacagt ttaaataatg attagaaaag tagaaatggc
     1801 agatgttgag gtgttggcta aaattgccaa acaagccttt cgtgaaacct ttgcgtatga
     1861 taatacggaa gagcagttac aggaatactt tgaagaggct tatagtctga aaactttgtc
     1921 aactgagttg ggaaatcctg actctgaaac ctatttcatt atgcatgagg aggagatagc
     1981 tggttttctc aaagtcaact ggggaagtgc tcaaactgag agagaattag aggacgcttt
     2041 tgaaattcaa cgcctctatg tgctacaaaa attccaagga tttggactag gtaagcaact
     2101 gtttgaattc gcacttgaac ttgctacaaa aaatagtttt tcttgggctt ggttaggtgt
     2161 ttgggagcat aatacaaaag ctcaagcctt ttataatcga tatggttttg aaaaatttag
     2221 ccaacatcat tttatggttg gtcaaaaagt agatacggat tggttactga gaaagaaatt
     2281 aaggtaagaa gaggattctt ctattgaaat gataggaaag gaaaagaact agagtgacaa
     2341 ctgtcattct ggagaactaa attatgcttg tatcttataa atggttaaaa gaattggtgg
     2401 acattgatgt gccatcacaa gagttggctg aaaaaatgtc aactacagga atcgaggtag
     2461 agggtgtcga atcaccagct gctggtctct caaaaattgt cgtcggtgag gtcttgtctt
     2521 gcgaagatgt gccagagact cacctccatg tttgtcaggt taacgttggc gaagaagaag
     2581 agcgtcagat cgtttgtggt gccccaaatg tgcgtgctgg gatcaaggtc atggtggctc
     2641 ttccaggagc tcgtatcgct gataactaca aaatcaaaaa aggaaaaatc cgtggtttgg
     2701 agtcacttgg aatgatctgt tcacttggtg aattgggaat ttctgactca gttgtgccta
     2761 aggaattcgc agatggcatc caaatcttgc cagaaaatgc cgtgccaggt gaggaagtct
     2821 tttcttacct agacttggat gatgaaatca tcgaactttc catcacacca aaccgtgcag
     2881 atgccctttc tatgtgtgga gtggctcacg aagtggcagc catctatgac aaggcagtca
     2941 actttaaaga atttactcta acagaaacta atgaatctgc ggcagatgcc ctttctgtca
     3001 gcattgggac agacaaggcg ccttactatg cagctcgtat cttggacaat gtgactatcg
     3061 caccaagtcc acaatggttg caaaaccttc tcatgaacga aggcatccgt cccatcaata
     3121 acgttgtaga cgtgacaaac tacatcctgc tctactttgg tcaacctatg catgcttttg
     3181 acttggacac atttgaaggg actgacatcc gtgtgcgtga agcgcgtgct ggtgaaaagt
     3241 tggtgacctt ggacggtgaa gaacgtgact tggacgtgaa tgacctagtc atcactgttg
     3301 cagacaagcc agtagccctt gcaggtgtca tgggtggtca agcaacagaa atctctgaaa
     3361 aatctagtcg tgttgtcctt gaagctgctg ttttcaatgg caaatctatc cgtaagacaa
     3421 gtggtcgcct gaaccttcgt tctgagtcat cttctcgctt tgaaaaagga attaatgtgg
     3481 caacagttaa tgaagccctt gatgcggcag ctagcctgat tgcggaactt gcaggtgcga
     3541 cggtgcgtaa gggcatcgtt tcagcgggtg agcttgatac ttcagatgta gaagtttctt
     3601 caacccttgc tgatgttaac cgcgtcctcg gaactgagct gtcttatgct gatgtagaag
     3661 acgtcttccg tcgtcttggc tttggtcttt ctggaaatgc agacagcttt acagtcagag
     3721 tcccatgtcg tcgttgggat atcacaatcg aagctgacct ctttgaagaa atcgctcgta
     3781 tctatggtta tgaccgcttg ccaactagtc taccaaaaga cgatggtaca gcaggtgaat
     3841 tgacagccac acaaaaactc cgccgtcaag ttcgtactat tgctgaagga gcaggtttga
     3901 cagaaatcat cacctatgct ctaacaactc ctgaaaaagc agttgagttt acggctcaac
     3961 taagtaacct tacggaactc atgtggccaa tgactgtgga tcgttcagtc ctccgtcaaa
     4021 atatgatttc aggtatcctt gataccgttg cctacaacgt ggctcgtaag aataaaaact
     4081 tggcccttta cgagattgga aaagtctttg aacaaacagg taatccaaaa gaagaacttc
     4141 caaatgaaat caacagtttt gcctttgcct tgacaggctt ggttgctgaa aaagatttcc
     4201 aaacagcagc agttccagtt gattttttct atgctaaggg aatccttgaa gccctatttg
     4261 ctcgtttggg actccaagta acctatacag caacatctga aatcgctagc cttcatccag
     4321 gtcgtacagc agtcatttca ctcggtgacc aagttcttgg tttccttggc caagtgcatc
     4381 cagtcactgc taaggcttat gatattccag aaacgtatgt agctgagctt aacctttcag
     4441 ctatcgaagc tacgcttcag ccagcggctc catttgtaga aatcaccaaa ttcccggcag
     4501 tcagccgtga cgttgccctt ctcctcaagg cagaagtgac tcatcaagaa gttgtagatg
     4561 ctatccaagc tgccggcgtg aaacgtttga cagatatcaa actttttgac gtcttctcag
     4621 gtgagaaatt gggacttggt atgaagtcaa tggcttatag cttgaccttc caaaatccag
     4681 aagatagctt aacggacgaa gaagtcgcac gctatatgga aaaaatccaa gcatcgctcg
     4741 aagaaaaagt caatgcagaa gtgcgttaaa cgatagtatg atacaaattc cccctctagt
     4801 ttatgaacac tagaggggtg attttatata aaataattaa aatgtgattt ctgtaaaaat
     4861 acatctatga tcagagtttt gaaaacttac aatgtctaaa tctttaacat agtagtggtt
     4921 tttaggcaat aaaatatgat ctattgttgc attaaaaagt tttggacttt ggctattcca
     4981 agttcctctt tcaaaaggtg gcagtacatt taatgcgtcc ctatgagagc ttatttttgc
     5041 aagtgctcca tgacgcatag ttgcattaaa atcacctgca ataatagcct ttggatattt
     5101 tgaagccaat tgattatgaa tgatgtttaa gtcttgcttc cagatttcca ttaaacctgg
     5161 cagaggagga gcagtatgca aggcaatgat atctggtata ttttgttttc tcgaatgtaa
     5221 tacaattgtc ccgaaccgtg ttgtatgaaa agttttagct tctgtataga aaccataact
     5281 tttcttgaca atcacagtca caggagctat tccactattg gtaggtggag aagtgaaaat
     5341 atcatagttg gccatagaaa gtccaacttg atgaaacaat agtttgattc tctggttttc
     5401 ttgctcacct ctgatattgg tagctagttc agggaatata gccatatcgg catcaaaatg
     5461 gctaaaaatt cgctcaatat gttgtgcttc tatttgatta gcgacgttcc aagttactaa
     5521 ttttaaggtt ttagtcttat ttgatgcaga agaaagggtt ttatctgttc cgaataatag
     5581 taatatgagc gatagtaaga gcaaaatgcc agagtaaaac cgtatttgtt tatttttctt
     5641 gtaaaaagat aggaaaaaaa atacgaaact taaaatagca gccatagttg cagtagctaa
     5701 ggggaaggca taaatctgtg ccaagatagg ttgggtagat agccaaatgt taagattaga
     5761 agatgggaaa aaagaataag ttatgatagg gatagaaaag agaatgaccg aaagggcaga
     5821 gagaaatgtt ttaatcattg tgtgagtacc tcaatttgca atggttgctc ggagctaatg
     5881 tgactttgtg actttacttg attgatattc tttttcttgt cttcctcata gattaagtac
     5941 aacccaagta aaaaaagtcc aatttaagat tgaaatgatt aaaaggcaca gaaatgcaag
     6001 acaattatac tacaaaaggc aaacatttga caatcgatag ccgtcgctta atcgaacgat
     6061 ggaaaaaaga aggaaaatca aatagagaaa ttgcctctct acttggaaaa gctcctcaaa
     6121 ctatccacac tgaaatcaag cgtgggacag tccgacaatg tcttggaaaa gggcgcttca
     6181 aagagattta ttctgccgac tacgctcaac agtcttaaga aactaaagga aaagattctc
     6241 cactatacat aaccaaaaat tttcgcctga aatgatggtg atggctaaag gggttaacgt
     6301 gggaatttca accatttact attggattca tcgtggaaaa ttggggttaa gcaagcagga
     6361 actgctttat cctagaaaag gaaaatcgct taagaaatag gctagcacca actttaaacc
     6421 cactggtcaa tctatcgaat cataatttgc tcattttttt aaaaatacat agacgtaaaa
     6481 aaaagagggt taagtgatga agaatagtgc tattgggagt aattggaagg atgtccatct
     6541 gagctcttta gcaaggagga aatccttgaa agtgatatgc gagtggctat catgagcgag
     6601 ttgattgagg ctaggtatga acaaggaatc agtcagaaaa agctggaaga agtcagtgga
     6661 ataagccagc ctgtcatagc taggatggag acaggaaaga ctagtcctca gttggataca
     6721 gtcttaaaag tcctagccag tttaggaaag acactagcaa tcgtcccact tgaacaggga
     6781 aaaagttgac tagaatcagt tatagtcttt tctaataaca agccatagtc acttataaga
     6841 attactaata acatgtttta gtattctaat tggaatatag ctgtaaagta agaattacaa
     6901 aggatttgag gcatttttct caggtctttt tcttttgttt aaaacagaga tatagtttca
     6961 aactatatca aagcctaatt ttttacaatt atacagatga tttcttttct gttaagatag
     7021 tttcaacaac aaattttgga ggacacatca tgtcaactac aatcatcggt ttccctcgtt
     7081 tgggcgaatt ccgtgaatta aaatttacaa ctgaaaaata ctttagaaaa gaaatctcag
     7141 aagaagaact tttagcagca gcaaaagact tgcgtgctaa acactggaac attgtcaaag
     7201 aaaaaggcat cactgaaatt ccatcaaatg acttttctca ctatgataac ttcctagatg
     7261 cggctttcct tttcaacgtg gtgcctgcat ccgttcaaaa cttggacttg tctgaccttg
     7321 agcgctactt cgctttggga cgtggttacc agggagaaaa aggggacgtt cgcgcccttc
     7381 caatgaagaa atggttcaac actaactacc actacatcgt tcctaaattt gaaaaagaca
     7441 ctcaagtaaa attggctggt cacaagattt tcgatgagtt ccaagaagcc aaagaacttg
     7501 gattgaacac tcgtcctgtt cttgtaggtc ccttcacttt ccttcaattg tcagactttg
     7561 aagaaggcgt gaaagcagac gacttcgtag atagcttagt ggctgcttac caagaagttt
     7621 ttgctaaatt ggctgaactt ggtgcgagtc gcatccaatt ggatgaagcg gctcttgtaa
     7681 aagacttgac agctgaagaa aaagctctct tcttgaacct ttacaacaaa ctcttggctg
     7741 acaaaaaagg tcttgaagtc ttgcttcaaa cttatttcgg tgatgttcgt gacgtatacg
     7801 ctgaccttgt aaacttacca gtagatgcta tcggtcttga ctttgttgaa ggtaagaaaa
     7861 ctcttgaact cgttaaaggt ggcttcccag ctgacaagac tctctatgta ggtattgtca
     7921 atggtaaaaa catctggcgc aacaactacg aaaagagctt ggctgttctt gagcaaatcc
     7981 cagctgaaaa catcgttttg acaagctctt gctcacttct tcatgtgcca tttacaactg
     8041 ctaatgaaga atttgaacca gcactcttga accactttgc ctttgcagtt gaaaaattgg
     8101 atgaaatccg tgacttggat gctatccgca atggtcaagg ttcagaagct cttgcagcca
     8161 acaaagaact ctttgcgact gagcgtgttg gtgaaaatgc tgaacttcgt gcgcgtatcg
     8221 ctggcttgac agacacagac tacactcgtt tgccagcctt tgcagaacgt gaagctatcc
     8281 aagaagaagc tttcaaactt ccagctcttc caacaacaac gattggttca ttccctcaaa
     8341 caaaagaagt tcgtgccaaa cgtttggctt accgtaaagg tgaattgtct caaaaagagt
     8401 acgatgcttt ccttgctgaa acgattgacg aatggatcaa gtggcaagaa gatattgact
     8461 ttgatgtcct tgttcacggt gaatttgagc gtaatgacat ggttgagtac ttcggtcaaa
     8521 acttgtcagg atacctcttc tctaaaaatg gatgggtaca atcatacggt atgcgtgggg
     8581 taaaaccacc aatcatctgg ggtgatgtca ctcgtcttaa ccctatcact gttaaatggt
     8641 ctagctatgc acaaagtcgt acaaacaaac ctgttaaagg tatgttgact ggacctgtta
     8701 ccatccttaa ctggtcattc ccacgtgaag acatctctat caaggattca actctccaaa
     8761 tcgcccttgc tatcaaggat gaagtacttg accttgaagc tgctggtgtg aaaatcatcc
     8821 aaatcgacga ggctgctctt cgtgaaaaat tgccactccg ccgtagcgac tggtacgaag
     8881 actaccttga ctgggctatt cctgccttcc gcttggtaca ctcaacagta gcgccagaca
     8941 cacaaatcca cactcacatg tgttactcag aatttacaga tatcatccca gctatcgata
     9001 acatggatgc agacgttatt tcctttgaag ctagccgttc aaaccttgaa atcttggacg
     9061 aactcaaagc gaaaaacttc caaacagaag tgggacctgg ggtttacgat atccactcac
     9121 ctcgtgtacc aaatgaaggc gaaatcgaca acacaatcga agccatcctt gctaaagtgc
     9181 caagcaagaa agtttggatc aaccctgact gtggtttgaa aacacgtggt atcccagaaa
     9241 caaaagaaag cttgatccgc cttgttgaag cagctaaagc tgcgcgtgag aaattgtaag
     9301 attggatttc tctccataca gtggttgttc aaaaagaaat caactagaaa aggttattac
     9361 atatgtcacg ccaaacaccg tcactctcat ttgaagtgtt ccctccaaac ccagccgtgg
     9421 gtaatggtaa cattatttca gccttgcagg atatgcagga gttggctccc cattttatca
     9481 gtgtaactgc cagcaataat aaatttaata tcaaggaaac gacggttcgt ttggctgact
     9541 ttatccaaaa tgatttggcg attccgacca ttgcccactt gccagctatt tatctaacta
     9601 aggacaaggt tgctgaaact attgctgact tggacaaagt tggggtgcag aaaatcttgg
     9661 ctcttcgtgg ggatattatt ccagacgtgg aaccacaaaa ggatttccgc tatgcaaccg
     9721 acttgattga gtttatcaag gaacaaaccc ctcactttga tattattgga gcttgctatc
     9781 cagaagggca tccagattcg ccaaatcaga tttcagatat tcaaaatctt aagaagaaag
     9841 tggatgcagg ctgttcgagc ctcgtaactc agctcttctt tgacaatgag cgcttctatg
     9901 attttcagga caagtgcatc ttggctggga ttgatgttcc cattcatgca ggaattatgc
     9961 caattctgaa tcgaaatcag gctctccgac tcttgaagac ttgtgagaat atccatcttc
    10021 cacgcaaatt taaagccatc ttagacaagt atgagcatga ccctgaatcg ctcagagcag
    10081 caggacttgc ctatgcagtg gaccaaatcg tggacttggt aactcaggat gttgccggtg
    10141 tgcatctcta tacgatgaat aatgctgata cagcaaaata catccatcaa gcaacccatg
    10201 ccttgtttaa tcaccagtct ctaggataat aaaagcaaac cattcttctc aggtgagggg
    10261 aatggttcct ttttaatggt aaagacctca ctttttaaga aaaatatgat aaaataaatt
    10321 ctgtacgtac ttgatacaaa gatgaaggta ttaaataata caatgcattt aaactgtaag
    10381 atttaacgac acgagtgagt caaatcgatg ttatttgacg agagagttag taaagcattt
    10441 agctaatcgc ccgatagcga ttagcgtgaa agctttagat actttagtat taaacctttc
    10501 taatgatgta tcttgaaaac tgaacttgcc tacaactctg tggaaaaaga taaatctccc
    10561 taggagcagt cgctccgtcg ttcgatttcc tattttcctt tgagttgctt gacggcttgg
    10621 tatcttgagt aattgaattt tggtctcatt tcttaggaaa aaagataagc ttcctagcac
    10681 tcgtcgagtg cgacgtcacc ttcctatttt tctacagaaa tgttcggcaa gccgaaccgt
    10741 ccaaaatatc ttgtgtaatt gaacacggcc gaaaagctgt gtaaaaaaga taaactgtct
    10801 tgtcttcatc gaagacttcg tcagtttcct atttttactt tgcttttgac gtccttggta
    10861 tcttgatctt tgtaggcaag gcgtataatt tcatcaatcc aaaggggatt aaaatggcaa
    10921 aacaagtgtt tcaaacgact tttgcgggtc gtgagttaat tgtagagact ggtcaggttg
    10981 ctaagcaagc aaatggctct gttgtcgtac gttacggtga gtcaactgtc ttgactgctg
    11041 ccgttatgtc taagaaaatg gcaactgggg attttttccc actccaagtc aactacgaag
    11101 aaaaaatgta tgcggctggg aagtttcctg gtggctttat gaaacgtgaa ggacgtcctt
    11161 caacagatgc gaccttgaca gcgcgtttga ttgaccgtcc gattcgtcct atgtttgcgg
    11221 aaggtttccg taatgaagtc caagtcatca atacagtgct ttcttatgat gaaaatgcat
    11281 ctgcaccaat ggctgctatg tttggttcat ctttggcact gtctatttca gatattccat
    11341 ttgacggacc aattgctggg gtacaagtgg gatatgtaga tggccaaatc atcatcaacc
    11401 caagtcaaga acaagcagag caatctcttc ttgaattgac agtagctgga accaagcacg
    11461 ctatcaacat ggtagagtct ggtgccaaag aattgtcaga agaaatcatg ttggaagcgc
    11521 ttcttaaagg gcacgaagct gtcaaagaat tgattgcctt ccaagaagaa atcgttgctg
    11581 ctgtcggtaa agaaaaagca gaagtggaat tgcttcacgt ggatgctgaa ttgcaagctg
    11641 aaatcattgc agcctacaac agtgacctcc aaaaggcagt tcaagtagaa gagaaattgg
    11701 cccgtgaagc tgcaactcaa gcagtgaaag accaagtgac tgccgtttac gaagaaaaat
    11761 atgcgaacca cgaagaattt gaccgtatta tgcgtgatgt ggctgaaatc ttggaacaaa
    11821 tggaacacgc agaagtgcga cgtttaatta cagaagacaa ggtgcgtcct gatggtcgta
    11881 aggtcgatga aatccgtcct ttggatgcgg ttgttgactt ccttcctcgt gtacatggtt
    11941 caggtctctt tactcgtggg caaactcaag ctctttcagt cttgaccttg gctccgatgg
    12001 gagaaactca aatcattgat ggtttggatc cagagtacaa gaaacgcttt atgcaccact
    12061 ataacttccc tcaatattct gtaggggaaa caggtcgtta cggtgcgcca ggtcgtcgtg
    12121 aaatcggtca cggtgccctt ggtgagcgtg ctcttgctca agtcttgcca agcttggaag
    12181 aattcccata cgctatccgt ctagtagcag aagttttgga atcaaacggt tcttcatctc
    12241 aagcttctat ctgtgcggga actcttgccc ttatggctgg tggtgtgcca atcaaggcgc
    12301 cagtagctgg tattgctatg ggacttatct cagatggaaa taactacaca gtattgacag
    12361 atatccaagg tttggaagat cactttggag atatggactt caaggttgca ggtactcgtg
    12421 atgggattac agcccttcaa atggatatca agattcaagg gattactgca gaaatcttga
    12481 cggaggctct tgctcaagcc aagaaagcgc gttttgaaat ccttgatgtc attgaagcaa
    12541 ccattccaga agttcgtcca gaattggctc caactgctcc gaaaattgat acgatcaaga
    12601 ttgatgtgga caagattaag attgtcatcg gtaagggtgg agaaaccatc gacaagatta
    12661 tcgctgaaac aggtgttaag attgatatag acgaagaagg aaatgtgtct atctactcta
    12721 gtgaccaaga tgctattaac cgtgccaaag aaattattgc tggtttggtt cgtgaagcca
    12781 aagtggatga agtttaccgt gctaaagtcg ttcgtatcga gaaatttggt gcctttgtta
    12841 acctctttga taagacagat gcccttgttc atatctctga gatggcttgg actcgtacca
    12901 atcgtgtaga ggatttggta gaaatcgggg atgaagttga tgttaaggtt atcaaaattg
    12961 atgaaaaagg ccgtatcgat gcctctatga aggctcttct acctcgtccg ccaaaacctg
    13021 agcatgatga aaaaggtgaa aagtctgagc gccctcaccg cccacgtcat caaaaggatt
    13081 acaaacctaa gaaagaattt acagaaacat caaaagattc agaataagaa aaggagaaat
    13141 gtatggggtg gtggcgcgaa accattgata ttgtaaaaga aaatgatcca gcggcccgca
    13201 ccactttgga ggttttgctg acttatccag gtgtcaaggc cttggcggcc caccgtctct
    13261 cgcattttct ctggaagcac ggcttcaaac tattagctcg tatgtacagt cagttttggc
    13321 gcttttggac tcagattgag attcatccag gagcccagat tgattcaggt gtttttattg
    13381 accatggttc tggtctggtg attggagaga cagcgattgt tgaaaaaggc gttcttctct
    13441 atcacggagt gactctcggg ggaacaggga aagactgtgg caaacgccat ccgactgtac
    13501 gaaagggagc tttaatatcc gctcatgctc aagtcattgg acccgttgaa atcggtgaaa
    13561 atgccaaagt cggtgcagca gcagttgtcg tggcagacgt gcctagtgat gtgacggttg
    13621 tcggtattcc ggccaagatt gtccgtgtac atggtaaaaa ggatgagcct gtcattcacg
    13681 aagtcgaaga aaaacgagag tactacgtca ataaactcga gcaggctaaa gaagccagtc
    13741 acagatcgtc tggtttgtag aggataccta tatgattcaa gcaagaaaca agttaagcca
    13801 agaagagcta tctgaggcga aaaaagtaat taactgttgc caaaactatg acggtaccta
    13861 tcgcgatccc tatctctcta acatgcttaa ttttgaccca aacatgcccg cctttttcct
    13921 ttattatgaa aaaggcgaac ttgttggttt attaactgtc tatgcagatg accaagatgt
    13981 agaagtgacg atactggttc atccaggtca tcgccgtcag gggattgcgc gtgcattgtt
    14041 tactagtttt gagagagaaa cagcttcttt ccccattcgg tcagtcactt ttcagacaga
    14101 acgtattttt ttagagaatc atcctgactt tgccagtaac tggggactaa tagaggatga
    14161 agagacagaa acttggttag gtaaggatag aagaccttat ccgttagcaa atgtttccca
    14221 tcttgaagtt ttgttagcag atagttcgta tcaggatcaa attagtcaat taaaatttca
    14281 ggcattttca gaggaacatg aatcgagaga agttgtggat agatatgtcg ctgaagctct
    14341 gaaggatcca gaaagtcgct tatatatttt gttaaaagac ggtcaggtta ttgggacttg
    14401 cacggtagat ttatcgacta atacgaatta cttctacggt ttagcaatat cagaacctga
    14461 acgtggaaaa ggctatggaa gctacttagc aaaatccctc gtcaaccaac taattgagca
    14521 aaatgacaag gaatttcaga ttgcagtgga agatagcaat gtaggtgcca aacgtttgta
    14581 tgaaaaaatt ggctttgtca aacagactca ggtggtttat ctgaatgaga aaggagcaag
    14641 ggattccgaa gtgtagagat attcggactg aaatttactt gaacttttag tgatgaaact
    14701 aattgttctt ggatttcagc tttcctgatt atgatttatg attaaaatct atgacaccat
    14761 gtctcgtgat ttgcgagaat ttgtcccgat tgaggacggc aagatcaaga tgtatgtttg
    14821 tgggccaacg gtgtacaact atatccacgt aggaaacgcc cgttcgacgg tagcttttga
    14881 tacgattcgt cgctattttg agtaccgtgg gtataaggtt gcctatattt ccaattttac
    14941 agatgtagat gataagatta tcaaccgtgc cagggaagaa ggtatcacgc ctcaggaggt
    15001 tgcggataag tacatcgctg cctttcgtga ggatgtgacg gcattgggcg tgaaacctgc
    15061 gactcgccat ccgcgtgtag tggagtttat ggcagacatc atccgttttg tggaagactt
    15121 gatcgagaaa ggctttgcct atgagagtca aggggatgtc tatttccgtg tagaaaaatc
    15181 ccacaactat gctaaattgg ctaataaaac cttggaagat ttggagctag gtgcttcagg
    15241 tcgtaccgat gaagaaacgg ctcgtaagga aaatcctgta gactttgctc tatggaaatc
    15301 ttccaaacca ggtgagattt cttgggatag tccttgggga cctggacgtc cgggttggca
    15361 tattgagtgt tcagtcatgt cgacagagat tttaggtgat accattgata tccacggtgg
    15421 tggagctgac ctagagtttc cacaccacac caatgaaatt gcccagtctg aagctaaaac
    15481 aggcaaggct tttgctaact actggatgca caatggcttt gtcaatatcg acaatgtcaa
    15541 aatgtctaag tccttgggta acttcattac ggtacacgat gcccttaaaa ctcttgatgg
    15601 gcaagtactt cgtttcttct ttgcgaccca gcactaccgt aagcctatca acttcacgga
    15661 aaaggcagtt cgcgatgccg agaccaatct caagtatctc aagaatactt acgaacaacc
    15721 atttacagga aatgtggatg cccaagagtt acaaaacttt aaagataagt ttgtagcagc
    15781 tatggatgaa gattttaatg ctgccaacgg tatcacagtt gtctttgaaa tggccaaatg
    15841 gatcaactca gggaactatg atgcaagtgt caagcaagct cttgcagata tgttagaaat
    15901 ttttggaatt gtctttgttg aggaagtttt ggatgcagag attgaagact tgattcaaaa
    15961 acgccaagag gcgcgtgcca atcgtgactt tgcgacagca gaccaaatcc gtgaccaatt
    16021 ggttactcaa ggaattaagc tccttgatac caaggatgga gtgaggtgga cacgtgattg
    16081 atgtcaatct cattaacggg attgcgctag cctttgaggg ggatgcggtg tattctatgt
    16141 atattcgccg tcacctcatc ctcaaaggta tgaccaaacc caataaactc catcaagaag
    16201 cgaccaagta cgtgtcagcc aaggctcagg ctcgcctgat tgctctcatg ttggaggagc
    16261 aggtcctaac ggaaaaagaa gaagaaatct acaaacgtgg ccgcaatacc aatagccaca
    16321 caaaggctaa aaatgcagat gtcgtgactt atcgtatgtc cacgggattt gaagcggtta
    16381 tgggctatct ccatatgact gagaatctgg aacgtcttga gagtttggtt tcatggtgca
    16441 tccaaaaagt ggagggctag aacatgaggg caaaagaact acaagactgg tttcctgagg
    16501 ctcggatttc agaccaacca gtagagaaag agggctatct cacgctccct ttagcttctc
    16561 agcagtggat tttgctggag gaagctgggc tcagcgagcg tgaaaagcag ttggttgccc
    16621 ttttgaccca gcaggagcag gctcgttcgc taaacccttg gtattcctat ctggttgagg
    16681 gcaagggaca ggcaccgcaa gtttttaaaa agattcagtt ggtttattgc catctttctt
    16741 attttcagca ggaaaatctg gcttcttggc tagatatgat gcggactctt tttccgaatt
    16801 gtcagacagt gctacaggtc ggagctcagg attatgtttt cgtgcttcaa caagacaaat
    16861 acacttctgt aagagatatt ttaagtgata cgattgaagc ggttgagtat gactttggac
    16921 ttcgtctttc tatcatgttg ggtcaggttt ggtctcagac gggacatcaa gccctatcag
    16981 acttaatcaa agctgagcgg gatttgttca agacatggtg gcgtcagggt caccaaggtg
    17041 ttcatacttt ttctcagctc tatctttgga gtatgggaga aagactcgtg gacttgaagc
    17101 caatcaagga atgtctacac cagatgattt tggatcaaga tcagattcag gaaatcattc
    17161 tctctctttg ggaaaatagt gctgttctca ctaaaacagc ccagcaactc tatctgcacc
    17221 gcaattctct ccaatacaag attgataaat gggaagagtt gacagggctt cagttgaaag
    17281 agttgaccga cctgaccttg tgttatcaat tgattttagg ttctttgtca actatagttg
    17341 gtttgtaaag aagttaatat ttggagaaga ggattgccat cttctccatt tttatgtgca
    17401 gagttatagt ggcttgatgc tgggaaagta cactgtgact gctaaaacat tcctagaagc
    17461 tgctttgatt tccctaatct atttatgcaa attttatgtt attttactat aacagttgtt
    17521 gctaagccaa ataatagtgg ggaagttcat ttagacgtaa gcattgaaga taatcaggga
    17581 ggtagtgggt ataatttcag ttctgtttca agtagctcac aaacagctaa atatgaagga
    17641 actgtttata ataacaattc atcattatat ataacgattg ataaaacgtc tgatgcaaca
    17701 gctcttttga aattaaagtt gaataatgtt gataatcaac ctgctactga agttcctagt
    17761 tcaggaatta ctgtaaaatt aaatgctaaa gataatgctg gaaactggac aagtgcttcg
    17821 aataaaaaag aagtaacagt aaaaattgtt tctgctaaac cgacatatcc agacaaaatc
    17881 ttagtgaaaa atcctgataa tataaaagat acagaaaaaa tgccattatt gaaaaattga
    17941 aagaggcaaa taaaaatcat ccagcaggag ctccaacctt tgctaaaggt gaaggagagc
    18001 atgcaaatga tattgtagca acttattcag atggtacaac ttattatgta ccgttaaatg
    18061 atgtgacaaa atatgcgagg tagtggctgt accactcact tattcacctc cccgtgattt
    18121 gtagtagtga taggctttct cactattatt ataaaacaaa ataaagatca caacactttt
    18181 tcattctgtg ttgtgccttg agtgaaacga aaggaatgaa ttataaatat gaaaagtata
    18241 gtcactagca tagatgagcg cttgcgtact cgcctacgag tgattatctg gaagcaat
//
LOCUS       DAVKZB010000029        17919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKZB010000029 DAVKZB010000000
VERSION     DAVKZB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.029"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            196..1965
                     /locus_tag="VXA80_000918"
     CDS             196..1965
                     /locus_tag="VXA80_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV0353439.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1989..3944
                     /locus_tag="VXA80_000919"
     CDS             1989..3944
                     /locus_tag="VXA80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0353440.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3946..4383
                     /locus_tag="VXA80_000920"
     CDS             3946..4383
                     /locus_tag="VXA80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353441.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4445..5581
                     /locus_tag="VXA80_000921"
     CDS             4445..5581
                     /locus_tag="VXA80_000921"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV0353442.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(6136..6885)
                     /locus_tag="VXA80_000922"
     CDS             complement(6136..6885)
                     /locus_tag="VXA80_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV0353443.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            7018..9354
                     /locus_tag="VXA80_000923"
     CDS             7018..9354
                     /locus_tag="VXA80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV0353444.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKMF
                     GASQGWSELEISTLLYYLLGSIGFLSVFRACMPFTFWRVLLIVWSVGGFLATALFLRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            9883..11346
                     /locus_tag="VXA80_000924"
     CDS             9883..11346
                     /locus_tag="VXA80_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV0353445.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPIEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSAFLTEGDKQRLQAKIQEINYE"
     gene            11339..11767
                     /locus_tag="VXA80_000925"
     CDS             11339..11767
                     /locus_tag="VXA80_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353446.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            11745..12194
                     /locus_tag="VXA80_000926"
     CDS             11745..12194
                     /locus_tag="VXA80_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353447.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            12222..12533
                     /locus_tag="VXA80_000927"
     CDS             12222..12533
                     /locus_tag="VXA80_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV0353448.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            12559..13644
                     /locus_tag="VXA80_000928"
     CDS             12559..13644
                     /locus_tag="VXA80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEV0353449.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            13655..14350
                     /locus_tag="VXA80_000929"
     CDS             13655..14350
                     /locus_tag="VXA80_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0353450.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            14387..16357
                     /gene="tkt"
                     /locus_tag="VXA80_000930"
     CDS             14387..16357
                     /gene="tkt"
                     /locus_tag="VXA80_000930"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0353451.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLMSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            16732..17919
                     /locus_tag="VXA80_000931"
                     /pseudo
     CDS             16732..17919
                     /locus_tag="VXA80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5339 a         3111 c         3903 g         5566 t
ORIGIN      
        1 caaaagttag attttttctg tctaactttt ggggtgtagt tcatgtacac ctgatatgat
       61 gcgttttata atttttaagc ctttttgccc agcctcgtca aaagtaatgt tttgacacaa
      121 aatctgtgac aaaactttag ttttaaaggt ttttaacttt gtatatacta gttttaagaa
      181 aaggaggatg atctaatgga agaaaaagta tcattgaaag tcagggttca aaaactaggg
      241 acatcgcttt caaatatggt tatgcccaat attggagcat ttattgcttg gggagtattg
      301 actgccctct ttatcgctga tggctatctg ccaaatgaac agttagctac tgttgttggt
      361 cctatgttaa cgtatttatt gccaatcctg attggttaca caggtggata tatgatccat
      421 ggccaacgtg gtgccgttgt aggagctatt gctactgttg gtgcaatcac aggttctagt
      481 gttcctatgt ttatcggagc tatggtaatg ggcccactgg gaggatggac tatcaagaaa
      541 tttgatgaga agttccagga aaaaattcgt cccggatttg aaatgttagt taataacttc
      601 tcagctggtc tcgttggttt tgcattattg cttttggctt tctacgcaat cggtccagtc
      661 gtatcgactc ttactggagc tgttgggaat ggtgttgagg ctattgtcaa tgctcgcctc
      721 cttcctatgg ctaatattat catcgaaccg gctaaagtcc ttttcctcaa taatgccctc
      781 aatcatggca tttttactcc tctgggagta gaacaggtag ctcaagctgg taagtcaatt
      841 ctcttcctat tggaagctaa tcctggacca ggcctgggaa ttctattagc ttatgctgta
      901 ttcggtaaag gttctgctaa atcttcttct tggggggcaa tggttattca tttcttcgga
      961 gggattcatg aaatttactt tccttatgtt atgatgaagc ctactctatt tttagctgct
     1021 atggcgggag gtatctctgg aacttttact tttcaactct tagacgctgg tcttaaatct
     1081 ccagcttcac caggttctat tattgcgatt atggctacgg cgccaaaagg tgtttggccc
     1141 catctaaatg ttcttttagg tgttttagtg gcagcagttg tttctttcct tgtagcagcc
     1201 cttattcttc atgcagacaa gtcaactgag gattcgctcg aagctgctca ggcggctacc
     1261 caagcagcta aggctcagtc taaaggtcag ttagtatcaa cttctgttga tgcagttgtt
     1321 tcgacagact cagtggaaaa aatcattttc gcctgcgatg ctggtatggg aagctctgct
     1381 atgggagcta gtattcttcg agataaggtt aaaaaagcag gtctagagat tccagtatct
     1441 aatcaggcaa tctcaaattt gcttgataca ccaaaaacat taattgttac tcaggaagaa
     1501 ctgacaccaa gagctaaaga caagagtcca agtgctattc atgtttctgt tgataatttc
     1561 ttagcgtccc ctcgttatga tgaaattgta gcttcattaa caggagcttc tccaatagca
     1621 gaaattgaag gagatatacc aacttcagca ccagtagata gtcaggaaat tgacctcaac
     1681 catattgatg ctgtagtagt tgcttatggt aaagcacagg gaactgcaac tatgggctgt
     1741 gaaacgattc gggctatctt tagaaacaag aatattcgta ttccagtttc tactgccaaa
     1801 atttcagaat taggtgaatt taattctaaa aacataatga ttgtaacaac tatttcttta
     1861 caggcagaag tgcagcaagc agcaccgaat tctcaatttc ttattgtgga tagtttagta
     1921 acaacaccag aatatgacaa aatggctgct agaatgtaca aatagaacta gaggtttcta
     1981 aattacgaat gctattaacc aaacgagaag aacaattatt gaaggctttc ctacatgtag
     2041 ggaagctttc aatgcaagat atgactgaaa tcttacaggt ttcatctaga acaatttatc
     2101 gaactttatc agatttgaca gatagcatgg agcaatatgg aatcgaaata acgaagcatg
     2161 ggaaatacta tattttgact ggagagttgg atgatttgcc gacagaactt gaagtgttag
     2221 ttgagtatag tccccaagaa agacaagagt tgattaccta tcgccttctg actgagagtg
     2281 gttttgtcac caatgaagca ttgcaagagt gcacgaaagt cagtaatgta actattattc
     2341 aggatatttc agatattgat aagcgtcttt tagactttga tctgaaaatt gaacgacaaa
     2401 aaggttatcg gatttctggt gattcagttg gtaagagaag atttttggct attttactga
     2461 caaactgtat ctcagtagca gatttttcaa ccggtaattt tgggagcttt gatattttag
     2521 aagcagatag aactaggctg gccagtcaga ttgttaataa gcaactgtca ggttttccag
     2581 atatggatgc taggatgaag atgttttttg cgatcttgtt atctcttata ggtcaggagc
     2641 aaaacattga aaattcacct aatactagta agcaggcttt ggaaatttct caaaaaattt
     2701 ttcaagctta ctctaagcag actgcacaat tttatagtat tcaggaaatt atctattttg
     2761 cgagcatctt ggatgaatta atcattaaac gtcaggacaa tccgctcttt acggagaaat
     2821 ttgatggtga atttttctac aatatttcaa atctgattga tacggtttcc atgtatacca
     2881 agattgactt ttttaaggac aaggttttat tcaattttct tttccatcat attcggctca
     2941 gtttaggcgt ccctatcctt tttcagggtg aaaatttgcc agaatctgtc cagattttag
     3001 ttgaaaggaa taaatttctt tatacagtca tcagtctttt agtgaatgat atttttccga
     3061 aatatcttca tacagagtat gagtatggca tgattgccct acattttatc tctagcttag
     3121 gccgtagtcc agagatttat ccagtccgtg ttttgctttt aacggatgaa cgtcgggtca
     3181 ctagagattt attagtcagt aaaattaaga gtgttgctcc ttttgtagag ttgatagata
     3241 ttcagtctct agtagattac cacagtattg atctcagtca gtatgattat attttatcta
     3301 ccaagccgct gactaatcag gaaatcgatg taatttctag ttttccaacc gtcaaagaat
     3361 tgcttgaatt acaggaacga cttcagtatg tacaggcaca tcgtacaatt gtcgcgcgtg
     3421 atgctatcgc tccagagaaa agttatgact tgcaagatta tttaatatct agtagtcagc
     3481 ttttgagtca attcgagttg gttcaattgg agaataatca atcatttgag cacacggtag
     3541 aacaaatcat ccaatatcag aagaatgtga gtgacagaga ttacctaaca agaaaattgt
     3601 tatctcactt ccagaatagt cctatggcta ttcctaatac tggtctggtg cttttacata
     3661 gtcagtctag caaagtaaca acaaatagtt ttactatgtt tgaactcaaa ctacctatct
     3721 ccgcattgtc aatgaaacga gaggaagaag aggtcaaaag gtgtctgcta atgctaatgt
     3781 ctaaagaagc tagcgaggaa gcgagagatt taatgacagc tatcagtcag tcgattattg
     3841 aaaatcatct ttatacagag atttacaaga cgggaaatca atccattatt tatcagatgc
     3901 taaatactat ttttaacgaa aaaattaaga aattggagaa ctaatatgaa acttgaaaaa
     3961 catttgatta agcttaataa acaattttct aacaaggagg aagctatttg ttattgtggg
     4021 caagttcttt atgagggtgg atatgttaat gaagactata ttgaagccat gattgagcga
     4081 gataaagagc tatctgttta catgggtaac tttatcgcca taccgcatgg aacagatgca
     4141 gcaaaaaagg atgtcctcaa gtctggtatt acagtcgttc aagtccctag aggggttgat
     4201 tttgggaatg tatctaaccc tcaagtggca acggttcttt ttggtattgc tggtattggt
     4261 aatgaacact tagaaattat tcagaaaatt tctatcttct gtgcagatgt ggataatgtt
     4321 cttaaactag cagatgctca gtcaaaagag gaagtattgc gcttatttga tgctgttgaa
     4381 taattgaatt tcgtcatttg tcatctagta tatatgtccc tcaaatagga aaaggagaaa
     4441 ttgaatgaaa cattctgttc attttggtgc cggtaatatc ggtcgtggtt ttataggtga
     4501 aattctattt aaaaatggtt tccatattga ttttgtggat gtcaataatc agataattca
     4561 tgctctaaat gaaaagggca agtatgaaat tgaaattgca cagaaaggac agtctcgtat
     4621 agaagtaact aatgtggctg gcattaatag caaagaacat cctgagcaag tcattgaagc
     4681 gattcaaaag acggatatta ttactactgc aatcggacct aatatactcc cttttatcgc
     4741 tgaacttcta gccaaaggaa tcgaagctcg ccgagttgca gggaatacac aggtattgga
     4801 tgttatggcc tgtgaaaata tgattggcgg gtctcaattt ctttatcaag aagtcaagaa
     4861 atatttaagt ccggaaggtt tgacatttgc tgataactac ataggttttc caaatgctgc
     4921 agtagacagg attgttccag cacaaagtca cgaagattcc ctttttgttg tggtcgagcc
     4981 ctttaatgaa tgggtcgtgg aaaccaagcg tcttaaaaat ccagatttac gtctaaaaga
     5041 tgtgcattat gaagaagatt tagaaccctt tattgagcga aaactttttt cagtcaattc
     5101 tggacatgca acttcagctt acattggtgc gcattatggt gccaagacaa ttttggaagc
     5161 tcttcaaaat cctaatatca aatctcggat tgaatctgta ttagctgaaa ttcggagtct
     5221 cttgattgcc aaatggaact ttgataaaaa agaattggag aattatcaca aagtcattat
     5281 agaacggttt gaaaatcctt tcatagtgga cgaggttagt cgcgtagctc gtactccaat
     5341 ccgaaaatta ggctataatg aacgattcat ccggccgata cgtgaattga aagaactcag
     5401 tttgtcatat aaaaacctac ttaaaacagt tggctatgcc tttgactatc gcgatgtaaa
     5461 tgatgaagaa agtattcgat taggtgaatt gttggctaaa caatcagtca aagatgttgt
     5521 tatacaagtt acaggtttag acgaccaaga attgattgag caaattgtag agtatattta
     5581 atctttttcg aaaatctctt caaatcaggt tagcatccat ctgcaacctc aaaacactgt
     5641 ttgagttatc tgcggtaatc tttctagctt gtctttgatt tttgttgtta tttataaggt
     5701 aaaagaagct ggacaaaaag tcttcaaaat cggaaaaagg cagcctatcg ggtgttcaaa
     5761 aatcttgata ggatgtcctt tattatggaa agccttattg gattttctcc tcagattgag
     5821 tttttgatca gctttatgag ataggttttg ctagagatgt agcccatcat gttattttta
     5881 tggagagtgg gaaaattgtt gaaaaaaata atgcccatca attctttagt cgtccaagag
     5941 aagaacgaac caagcaattt tggaacgaat tttttcgaat gcgatctata tagtaaaatg
     6001 aaacaagaac aggacaaatc gatcaggaca gtcaaatcga tttctaaaaa tgttttagaa
     6061 gtagaggtgt actattctag ttccaatcta ctatataaat gaaaacctta gctgtttggc
     6121 caaggttcat ttcttctatg caagttcttc tcttttctta tctgggttcc agatgaagct
     6181 tgcgataaag ctaaagatga tggttaggat agcgaggata atagcaagga tgagggcagg
     6241 gatgcggata aaccaccaga gtggatttgg ttttttatca ttgtgccatt gtttcgtttc
     6301 ttcgtccaga cgttggaatt ctgtttgaat acgattggcg actgtttcaa tgccttcatc
     6361 attcattttc ttgatatctg agatatcgat tggatttcca aagttcatat cgacacgttc
     6421 acggctaatc aagcccttca aagtcatggg accggtgtag gtaaccggca tgatacggac
     6481 cttggccatt ttggcaatca gtgctgcgcc ccccttgaca tcgtttgagt ggcggctacc
     6541 acttggaaac atgatgagag agcggtcact ttttttgaga acgttgatag gatatttgat
     6601 ggctgaggcg ctgggatttt cacggtcgat gggaaaggcg ccacacatac gaatccacca
     6661 accaaagata cggttggtaa agagttcttt ttttgccata aagatgaact gttttggctt
     6721 ggtcgcaaag gccatataaa caggatccca ccaggtacgg tgaggcgcaa ctaaaatata
     6781 attttcatct tgattaggaa ttttatcagt attatgatag tgagcattgc cattgatgga
     6841 ccataagagc aatacaacta atccacgcaa ataagtataa aacatgcgat ctccttcgat
     6901 tgttttcttg ttattattat accttatcaa aggagggctg gcaaactttt cccttgacta
     6961 gatgcatatt tgggatgaaa ttagaattct gttaaaaaaa atgatataat agaatttatg
     7021 gataaaaata agattatggg attaacccaa agagaagtca aggaaagaca ggctgagggt
     7081 ttggtcaatg actttaccgc atcagccagt accagcactt ggcaaatcgt taaacgaaat
     7141 gtctttactc tttttaacgc tttgaacttt gccattgctt tggcccttgc ctttgtgcag
     7201 gcttggagca atctggtctt ctttgctgtt atctgcttta acgctttttc tgggattgtg
     7261 accgagctac gagccaaaca catggtggac aagctcaatc tcatgaccaa ggaaaaggtc
     7321 aaaaccatcc gtgatggtca ggaagttgct cttaatcctg aagaattagt gctaggagat
     7381 gtcattcgtt tgtctgcagg agagcagatt cctagtgatg ccttggtttt ggaaggcttt
     7441 gcggaagtca atgaagccat gttaacggga gaaagtgatt tggtgcaaaa ggaagttgac
     7501 ggcttacttt tgtcaggaag tttcctagcc agtgggtcag ttttatctca agttcaccat
     7561 gtcggtgcag acaactatgc tgccaaactc atgcttgagg ctaagaccgt taaacccatc
     7621 aactcccgta tcatgaaatc gctggacaag ttggctggtt ttactgggaa gattatcatt
     7681 ccctttggtc tggctctctt gctggaagcc ttgcttttaa aaggcctgcc tctcaagtca
     7741 tctgttgtaa actcgtcgac agctcttttg ggaatgttgc ctaagggaat tgcccttttg
     7801 accattactt cgctcttgac tgcagtgatt aagcttggct tgaaaaaggt cttggtgcag
     7861 gagatgtact ctgttgagac cttggcgcgc gtggatatgc tctgtctgga caagacgggt
     7921 accatcaccc aaggaaagat gcaggtggag gctgttcttc cgttgacgga aacgtatggt
     7981 gaagaggcta ttgccagcat cttgactagc tacatggccc atagtgagga taagaatcca
     8041 actgcccaag ccattcgcca gcgttttgtg ggagatgttg cttatcctat gatttccaat
     8101 cttcccttct cgagcgaccg caagtggggg gctatggagt tagaaggctt ggggacagtt
     8161 ttcttagggg cacctgagat gttgctggat tctgaagtcc cagaagccag ggaggccttg
     8221 gagagaggat cacgtgtctt ggtcttagct ctcagtcagg agaaattaga ccatcacaaa
     8281 ccacagaaac catctgatat tcaggctcta gccttgctgg aaatcttgga ccccattcga
     8341 gagggagcag cagagacgct ggactatctc cgttctcagg aggtgggact caagattatc
     8401 tctggtgaca atccagttac ggtgtccagc attgcccaga aggctggttt tgcggactat
     8461 cacaactatg tagattgctc aaaaatcacc gatgaggaat tgatggccat ggcggaggag
     8521 acagctattt tcggacgtgt ttcccctcat caaaagaaac tcatcatcca aacgttgaaa
     8581 aaagcgggtc atacaacggc tatgacaggg gacggggtta atgatatctt ggcccttcgt
     8641 gaggcggatt gttctatcgt gatggcggag ggggatccag caacccgtca gattgccaat
     8701 ctggttctct taaactcaga ctttaatgat gttcctgaga ttctcttcga gggtcgtcgc
     8761 gtggtcaata acattgccca catcgccccg attttcttga taaagaccat ctattccttc
     8821 ctgttagcag tcatctgtat tgccagtgct ttactaggtc ggtcagagtg gattttgatt
     8881 ttccccttca ttccgatcca gattaccatg attgaccagt ttgtggaagg tttcccacca
     8941 ttcgttttga cttttgagcg aaatatcaaa cctgtcgagc caaacttcct cagaagatcc
     9001 atgcttcgtg ccctaccaag tgcccttatg gtcgtattca gcgttctctt tgtgaaaatg
     9061 tttggcgcga gtcaaggttg gtctgagtta gaaatctcaa ctctactcta ttatctacta
     9121 gggtctattg gtttcttgtc cgtatttagg gcctgcatgc cattcacatt ctggcgtgtc
     9181 ctcttgattg tctggtcagt aggaggcttc ctagcgacag ctctcttcct aagaatccaa
     9241 aaactgcttg aaatttcaac cttaacagga caaacattac ctgtttatgg tgtcatgatg
     9301 ttggtcttta ccgtgatttt catcctgacc agtcgttacc aagcgaaaaa ataaagaaag
     9361 actgcaatct gtggattgca gttttttata cgatggatct atcgtagatc tgatgtgcaa
     9421 ggcctacatg tctattatct atcggtgaac ccaagagtga ttctcaagtc tgcttggatt
     9481 gaggtaatag attcaaatat ctgtagttag actatttgaa gtttgatgta agaaagagaa
     9541 agcgacagat tgaagtaatt ttaactctct tctattgcta gaacaagtgg tcggataggt
     9601 tggtagtttg aaaatgaaga tgctatctat tgttaaatgg aacatagtgt tatttattag
     9661 aaaatcgttt ggtttatttc ttatcaaata cgaaaagcaa cttaaatatt tcaactaaaa
     9721 tagatgttat gaagaaaagg taaaatgatt ttggcatagt gaggttctgt tctatttgat
     9781 atcatatttt tgataaaaac aaaaatgtcc attgcaaagg acaaaatgcg aagtatatta
     9841 ttttttgaaa gcgatataat ggaatcataa aggaggtgta tcgtgtctag aaaacaagaa
     9901 caaatggaaa cgttgttgct ccttttgcga gatagtaagg attatatatc tgctaaagta
     9961 ttgggagaaa aattaaattg ctctgataaa acggtttatc gccttgtcaa gggaatcaac
    10021 aaagattgtc cgatagaagc attcatttta tctgaaaaag gcagaggttt caaattaaat
    10081 ccaagaagtt ccctcgtgga cgttgatggg aattttacag aggcttttga tcctgaagta
    10141 aggcgtgaaa aattactaga acgtctcttg ttgactgctc ctaagccaca ttctatttat
    10201 gatttaggag aggaattcta cgtaagcgag tcagtagtac taaaagatcg tcagatatta
    10261 caagagagtc tagcaattta tgggttagat ttaaaaatga gacaacgaaa gctttttatt
    10321 gatggggatg aggctcaaat tcgttcagcc attctaaatc tactgccaat gtttaatcag
    10381 ttggatttag agcaaattac acagaataag gttcagcctc ttgacggaga acttgctcac
    10441 ttttgtttgg gattactgat tacacttgag agagaattgg gggtaaacat tccctatcca
    10501 tataatataa atattttctc tcacctgtat atttttatca gtaggaatcg tcgtagtact
    10561 agtattcatg ttgtagcacc ttcaaaacct actattgttg atgagaaaat ttacagtgtc
    10621 tgtcaaaaaa ttattcaaga aattgaacaa tattttagga tgaaggttga tgcagttgag
    10681 attgactatc tttatcaata cgttgtatct tcgagattgc aaaaaccatt ttcttccggg
    10741 aagcttcctt tttctcagcg agttttagat gtcactcatt actattttag ccgtatgtgt
    10801 atggacaata gagagattga aacgacagat cctgactttg ttgacttggc gagtcatatc
    10861 agtcccttac tgaggagatt agataataga gtacagatta agaatagtct tttatcacaa
    10921 attcttttaa cctatcctaa tctggttaaa gagttaacaa ctatttctaa agaagtgagt
    10981 ttagtatttg gttttgcgtc cttgagtctg gacgagattg gttttctagt cttatatttt
    11041 gcacggtttc aagaaaagcg agcacgtcct ctaaaaacag tagtgatgtg tacatcaggt
    11101 gtcggaactt cagagctttt acgagcacga ttagaaaagc aattttctga attggatatt
    11161 attgatgtag ttgcttatca tcaattagat gagctgataa atctatatcc agatttagat
    11221 ttcattgtga cgacggtagc tttgcaggaa ccagcaagtg tcccgtttgt cctagttagt
    11281 gcttttctaa ccgagggtga taaacaacgt cttcaagcaa aaattcagga gataaactat
    11341 gaataatctt tcgcttgtcc ttatggatat atctgttcaa aatcgtcaag aagcctacaa
    11401 agaattagca aatcaaatca gccttcttgt ttctgaagat acagaaaaaa tagaagagct
    11461 tctatattac cgtgagagac agggaagtat agaggttgct aaaggtgttc ttctaccaca
    11521 ttgtgaagga aactttcaac atcatgtctt agtgattact agattaaaac cacctatcag
    11581 agaatggtcg aaggatatcc agtgtgttga ccttattatc ggtttggcca ttgcagtatc
    11641 acaggacaag tcatgtatta aaacattgat gagaagacta gcagatgaat cattcataaa
    11701 tcaattaaaa cagttaacaa aagaagaatt acgggagata atatatggaa atcaaagata
    11761 ttcttaatgt gagtctgatc cagacggatt tacagatgca gagcaaagaa gaggtttttg
    11821 aggcattagc tcaactattg gttgagacgg gttatgtgtc tgatagagac caatttatcg
    11881 aaggtcttta tcagagagag gcagaaggac agactggtat tgggaattat attgctattc
    11941 cccatagcaa gagttctgct gtggagaagg cgggggtagt catagctata aatcacaatg
    12001 agattccttg ggagaccatt gatgggaaag gggtcaaagt aattgtactc tttgcagttg
    12061 gtgatgatac agaagctgct agggagcatt tgaagacctt atcactcttt gctcgaaaac
    12121 ttggtaatga cgaagttgtt gccaaattag ttcgggctca gacatctgat gatgtgattg
    12181 cagctttttg ttaataagaa aaaattttgg agggtatccg tatgaaaatt gttggtgttg
    12241 cagcttgtac tgtgggaatt gcccacactt atattgcaca ggaaaaatta gagaatgccg
    12301 caaaggtagc tggacatgtg attcatgttg agactcaggg gacaataggg gtagaaaatg
    12361 aattgagtca agagcagatt gatgcagcgg atgtagttat tttagcagtt gatgttaaga
    12421 tttctggtat ggaacgcttt gagggtaaaa agattatcaa ggttccaaca gaagtggcag
    12481 tcaaatctcc caataaactg attgctaaag ctgttgagat tgttacgaaa taactgaaaa
    12541 tatttaagga gaaaatatat gttgaaacac ttaaacttaa aaggtcactt attgacagcc
    12601 atttcctata tgattccaat tgtttgtggt gcaggattct tagttgccat tggtttagca
    12661 atggggggtg gtgttcctga cgctcttgta gcaggaaaat tcactatctg ggatgcttta
    12721 gcaactatgg gtggtaaagc ccttggtctc ttgccagttg ttattgctac aggtttgtct
    12781 tactcgattg ctggtaagcc agggattgca ccaggttttg ttgttggtct aattgccaat
    12841 tctgttggtt cagggtttat cggtggtatc ttgggaggtt atatagctgg tttcttggtt
    12901 caagcgatta ttaaaaaggt caaagtacca aactggatta aaggtttaat gccaaccttg
    12961 attattcctt ttgtagcctc tttggtaagt agtttgatta tgatttatat tattggagcg
    13021 cctatcgcag cctttaccaa ctggttgacg agcttattac aaagcttggg aagtgcttca
    13081 aatggtttga tgggggcagt tattggagtt ctcagtgctg ttgactttgg tggcccactt
    13141 aataaaacag tctatgcgtt tgtgttgact ttacaggctg aaggtgtgaa agaaccattg
    13201 actgctttgc aattggtgaa tactgctaca ccagttggat ttggattggc ctattttatc
    13261 gcgaaattac tcaaaaaaaa tatctatact caagaggaaa tcgaaacatt gaaatcggct
    13321 gttcctatgg ggattgtcaa tattgttgaa ggtgtaattc cgattgttat gaataacttg
    13381 gttccaggtc tcattgcaac aggtatcggt ggtgctgttg gtggtgctgt ttctttgaca
    13441 atgggtgctg attctgctgt gccatttggt ggggtgctta tgttaccaac catgactcgt
    13501 ccagtagctg gtatttgtgc tttgttagcc aacattgtag tcacaggact tgtctacgcg
    13561 attttgaaaa aaccaataaa acatacagaa ccagttatga ctgttgaaga agagattgat
    13621 ttgtcagata ttgaaatttt gtaagagggt aacgatgtca agaattgaat tttcaccatc
    13681 tttgatgacc atggatttgg acaaattcaa agagcagatt acttttttga atgataaagt
    13741 agcatcttat catatcgata ttatggatgg ccattttgtt cccaatatta ccttgtctcc
    13801 ttggttcatt caagaagttc aaaaaattag tgacacacct ttatcagttc atctgatggt
    13861 cacagaccca accttttggg tagatcaagt tctcgattta caatgtgagt atatttgtat
    13921 tcatgctgaa gttctgaatg gtcttgcttt tcgtttgatt gataaaattc atgatgcagg
    13981 tctaaaggct ggtgttgtcc ttaatcctga aacacctgtt tctacaatct ttccctacat
    14041 tgatttactt gacaaagtaa ctattatgac tgtagatcca ggttttgcag gacaacgctt
    14101 tttggagtct accttgtata aaatccaaga actccgtcag cttagagttc agaatggtta
    14161 tcactacatc attgagatgg atggttcttc gagtcgtaag actttcaaac aaattgatgt
    14221 ggcaggacca gatatttatg ttataggtcg cagtggatta tttggtttgg atgacgatat
    14281 tgccaaagcc tgggatatct gttctagaga ttacgaagaa atgaccggaa aaacaatgcc
    14341 aatcaaataa tggtttgaga agaaatttat tagttaggag gaatatatgt cactacaatc
    14401 agttaacgcc attcgttttc ttggcgtaga tgctattaac aaatctaatt ctggtcaccc
    14461 gggaatcgtc atgggtgctg cgccaatggc ttatagccta tttacaaagc accttagaat
    14521 tacacctgag cagccaaact ggattaaccg agatcgcttt atcttgtctg cgggtcatgg
    14581 atcaatgcta ctgtatgctc tcttgcattt aacagggtat aacgatgtat ccatggatga
    14641 gattaaaaat ttccggcaat ggggatctaa gacacctggt catcctgaag tgacgcatac
    14701 gtctggtgtg gatgcgacat ctggtccgct tggtcagggg atttctactg ccgttggttt
    14761 cgcccaagca gagcgttttt tagctgctaa gtacaacaaa gatggtttcc ctatttttga
    14821 ccattatact tatgttatcg ctggagacgg tgacttcatg gaaggagtgt ctgcggaggc
    14881 ggcttcttat gcaggtcatc aagctttaga taagcttatc gtcctctacg actccaacga
    14941 catctgcttg gatggtgaga ccaaagatac tttctctgaa aatgttcgcg cccgttacga
    15001 tgcttatggt tggcatacag ttctggtaga agatggaaca gatttagcag caatttctac
    15061 agcaattgag acggccaagt tttctggtaa gccgagtttg attgaagtga aaacggtaat
    15121 tggttacggc tcacccaata aaagtggtac aaatgctgtt catggtgcac cactaggagc
    15181 agaagaaaca ggagcaactc gtaagttttt gggatgggat tacgatccat ttgaagtacc
    15241 agaggaagta tattctgatt tcaagacaaa tgtagcggat cgtggtcagg aggcatacga
    15301 tgcttgggct agtttgatgt ctgattacaa ggttgcttat cccgaagttg ctagtgagat
    15361 tgacgctatt gtagctggaa aatcccctgt aaccattact gaaaaagact tccctgtcta
    15421 tgagaatggc ttctctcaag caactcgtaa ttcgtcccaa gatgctatta atacagcagc
    15481 agcagtttta ccaaccttct taggtggatc ggcagactta gctcactcca acatgaccta
    15541 catcaaggca gatggcttac aagataaata taatccatta aaccgcaata ttcagtttgg
    15601 ggtacgtgaa tttgccatgg gaacaatcct caatggaatg gctcttcatg gtggtttacg
    15661 agtttatggc ggaaccttct ttgttttctc tgactacgtc aaagctgcta ttcggctatc
    15721 agccattcag gagttgcctg taacttatgt ctttacccat gattcaattg ccgttggtga
    15781 agatggtcca actcatgaac cagttgaaca tttggcaggt ttacgctcaa tgccaaactt
    15841 gactgttatc cgtccagcgg atgcccgtga aactcaagcg gcttggcatc atgccttgac
    15901 cagtaccacc actccaactg tcattgtctt aacccgtcaa aacttggtag ttgaagaagg
    15961 gacagacttt ggtaaggtcg ctaaaggagc ctacgtcgtg tatgataccc cgggatttga
    16021 tactattatc attgctacag gatctgaggt caatctagct atcaaagctg ctaaggaatt
    16081 ggttttacaa ggtggtaaag tacgtgtggt atctatgccc tcaaccgaac tatttgatgc
    16141 tcaagatgct acctacaagg aagacatttt accatctaag actcgtcgtc gtgtggccat
    16201 tgaaatggca gcgacccaaa gttggtacaa gtatgttggt ttggatggcg cggtcatcgg
    16261 tattgacatc ttcggtgcgt ctgccccagc tcagactgtg attgataatt atggatttac
    16321 ggtagagaat atcgttgctc aagttaagtc cctatagaaa ccaattacaa tgaagataca
    16381 gctgttgtca gactagcaga tgtagtgata gacactaatc agatgattgg ttatttaaaa
    16441 actgtaatga aaatgtaata atttatctac gaaagttata gtagatagta tacacaatag
    16501 agtataccct gaaacggttg cgaagtacgc taatcacttt gctactgatc tagatagttt
    16561 ctttaatcaa taaacacagc atccacagat tgacttagga tattgtaagt tttttgaaag
    16621 ctagagagaa ggtctctaaa attaaaaaac gcatagtata ggatgttgaa atgatgaact
    16681 gcaccccaaa agttagacag aaaaaaatct aacttttggg gtgtttttat tatgaaatta
    16741 acttatgatg ataaagttca gttctatgaa cttagaaaac aaggatatat cttagagaag
    16801 ctttcaaata aatttgggat aaataattct aatcttaggt acatgattaa attgattgat
    16861 cgttacggaa tagagttcgt caaaaaaggg aaaaatcgtt actattctcc tgatttaaaa
    16921 caagaaatga ttaataaagt ctgacatgaa ggctggacta aagatagagt ttctcttgaa
    16981 tacggtctcc caagtcgtac gatacttctt aactggctag cacaatacag gaaaaacggg
    17041 tatactattg ttgagaaaac aaaagggaga gtacctgaga gcggagaatg ccatcctaaa
    17101 aaagttaaga gaactccgat tgaagaagga aaaagagaaa taagaaagac agaaattgtt
    17161 caagaattaa tgactgagtt ttcgttagtt cttcttctaa aatccattaa actagctcgt
    17221 tggacctact actatcactt gaaacagcta gacaaaacag ataaggacca agagcttaaa
    17281 gctgaaattc aatccatttt tatcgaacac aagggaaatt atgcttatcg tcggatttat
    17341 ttagaactaa gaaatcgtgg ttatctggta aatcataaaa gagttcaagg cttgatgaaa
    17401 gtactcaatt tacaagctaa aatgcgacag aaacgaaaat attcttctca taaaggagac
    17461 gttggcaaga aggcagagaa tctcattcaa ggccaatttg aaggctctaa aacaatggaa
    17521 aagtgctata cagatgtgac agaatttgcc attccagcaa gtactcaaaa gctttactta
    17581 tcaccagttt tagatggctt taacagcgaa attattgctt ttaatctttc ttgttcgcct
    17641 aatttagaac aagtacaaac aatgttggaa caggcattca cagagaagca ctacgagaat
    17701 acgattctcc atagtgacca aggctggcaa taccaacacg attcttatca tcggtttctt
    17761 gagggtaagg gaattcaagc atccatgtca cgtaagggta atagcccaga caatggtatg
    17821 atggagtcct tctttggcat tctgaaatcg gagatgtttt atggttatga gaagtcattt
    17881 cagtcgctta agcaattgga acaagccatt gtagactat
//
LOCUS       DAVKZB010000030        17621 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKZB010000030 DAVKZB010000000
VERSION     DAVKZB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.030"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(144..758)
                     /locus_tag="VXA80_000932"
     CDS             complement(144..758)
                     /locus_tag="VXA80_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV0353452.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VXA80_000933"
     CDS             complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VXA80_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV0353453.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1983..2612)
                     /locus_tag="VXA80_000934"
     CDS             complement(1983..2612)
                     /locus_tag="VXA80_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0353454.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKLIELFNQLLAGTQTRFLVIGGAGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            3471..3794
                     /locus_tag="VXA80_000935"
     CDS             3471..3794
                     /locus_tag="VXA80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV0353455.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSKNIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3852..5795
                     /gene="thrS"
                     /locus_tag="VXA80_000936"
     CDS             3852..5795
                     /gene="thrS"
                     /locus_tag="VXA80_000936"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV0353456.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVNYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5844..6818)
                     /locus_tag="VXA80_000937"
     CDS             complement(5844..6818)
                     /locus_tag="VXA80_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0353457.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALVFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6811..7488)
                     /locus_tag="VXA80_000938"
     CDS             complement(6811..7488)
                     /locus_tag="VXA80_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0353458.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGVEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7644..8717
                     /locus_tag="VXA80_000939"
     CDS             7644..8717
                     /locus_tag="VXA80_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV0353459.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8808..9110)
                     /locus_tag="VXA80_000940"
     CDS             complement(8808..9110)
                     /locus_tag="VXA80_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353460.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9179..9616)
                     /locus_tag="VXA80_000941"
     CDS             complement(9179..9616)
                     /locus_tag="VXA80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV0353461.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9692..10534)
                     /locus_tag="VXA80_000942"
     CDS             complement(9692..10534)
                     /locus_tag="VXA80_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0353462.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10646..11296
                     /locus_tag="VXA80_000943"
     CDS             10646..11296
                     /locus_tag="VXA80_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV0353463.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11473..12119
                     /locus_tag="VXA80_000944"
                     /pseudo
     CDS             11473..12119
                     /locus_tag="VXA80_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12250..12663
                     /locus_tag="VXA80_000945"
                     /pseudo
     CDS             12250..12663
                     /locus_tag="VXA80_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12682..12869
                     /locus_tag="VXA80_000946"
                     /pseudo
     CDS             12682..12869
                     /locus_tag="VXA80_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13136..13579)
                     /locus_tag="VXA80_000947"
     CDS             complement(13136..13579)
                     /locus_tag="VXA80_000947"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV0353464.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGVAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13609..15831)
                     /locus_tag="VXA80_000948"
     CDS             complement(13609..15831)
                     /locus_tag="VXA80_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV0353465.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16826..17455
                     /locus_tag="VXA80_000949"
     CDS             16826..17455
                     /locus_tag="VXA80_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0353466.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVCETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFHFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLSNYDVYPGHGPATTIAHEKAFNPFF"
BASE COUNT         5225 a         3982 c         3234 g         5180 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatgc tcaatgaaaa agccctctga aaatcagaga gctgatttga gctcgggcta
      121 aaatcctaat gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagag gataacaccc gactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaacctc ctgtattagt
      781 cagtaataaa tcagcagatt tcaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatgtcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctagg gggcttgcat acagctactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccatttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat tttagcccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg cgacgaggcg aagatcgtac gaaactttca gtgaaacaaa aaaatctccc
     1321 cgaagggaga aaagctggtt tattttacct tacagtgcta ggaaccgtaa ccgaagatta
     1381 tacttgagta tagcgaggta aggtgacaac gcaaaaaagc tacccaattt agagtagctt
     1441 tattatcaag atatgctcaa ttgaacacgg cctaagcact tggcaaaaaa gataaaatct
     1501 cctagaaact gaagtttctt cgtcaatttt cctatttttg ctttgtgctt ttgacggcct
     1561 ttgtatcttg aattagcgac gaagtcctag agagttgatc aactcacggt aacggttaac
     1621 gtcgttttta cgcaagtatg caagcaagtt acgacggcga ccgatttttt tcatcaatcc
     1681 acggtaagta gcgtggtctt ttttgtgttg tttgatgtgt tcgttaaggt ggttgatttc
     1741 ccaagtaagg acagcaactt gaacctctac tgaacctgta tcaccttcgt gacgtgcata
     1801 ttgtgcaatg atttcatttt ttttctcttt tgagattgcc atgatgtttc tcctttttat
     1861 ttggcttcat ccgagtgaca ggttggcatg cctgtaacca agaagaagtt atttgtcttt
     1921 acgacaatcc ctattttacc aagatttaaa ttttctgtca aacattaata ctctcatagt
     1981 tattattttt caagtaaaga gatacgttct tttatatgat gacctttttc aagttcatct
     2041 accaatccaa tagcataatc tgcataacta atctggctta ttccttttcc attagtagta
     2101 aaaatttctc ctgctagaat gtagtttcct gttttttcac catcgggaat aaaatctaca
     2161 gcaggactga caaaagtcca gttgagatta ttttttgtac gaagaagatc caattcatcc
     2221 gcttgagctt ttgctagtga cttaaattct tcaggaaaat caggcgtatc taaaagtcgt
     2281 gtcgtttttg tttcatctat ataaaggctt cctgctcccc ctataactag aaaacgtgtc
     2341 tgtgttccag caagaagctg attaaatagt tcgattagtt tgctgtggag cggtagcgta
     2401 tctggtgtat aagcaccaaa cgctgaaata acagcatcaa atccagtaag atcatctttt
     2461 gtcaactcaa ataaatcttt tttaataata gactcagctt gacttttgtt ttcagaacga
     2521 acaatagccg ttacttcatg tcctcgtttg actgcttctt caacaattgc cttccccgct
     2581 tgtccatttg ctgcaataac tgctagtttc attttttata cctctcttgt tgtaattatt
     2641 ttagttacag caattgtgac actcttaata atcaatgtca atagtcttgc ttaattatta
     2701 tcaaaatatt tctaccaaga aaactaacca tgattctagt gaaaagaaaa atcttctttg
     2761 tcaacaaatt tactttcttg ttttaaacat gctataataa tcatagcaag agatctaagt
     2821 tgtctgtttt tttaaaacga ggtgattatc atgcgtagat tctattccca tctcccctac
     2881 tatctggtca tattattctt ttattggcca ctttatgagt tgttcttgct agttgtttct
     2941 gaccccctta cactatataa acaatcttct cttctttaca cctctggtaa tcttgattgt
     3001 atcgttactc tatagctacc gtttccgttt ctcactttga tggttagttg gtaacggact
     3061 gctcttttac tttactatca taacctttgg tgagtttata ctaatttact tgctaatcta
     3121 tgaaacagtt gctctggtgg gcatggattc tggtattagc atcaagcata ttctacaaaa
     3181 tatgaaaaac aaaaaacttt cacaaaatcc ttgaaaaatc tcacaatcat gctataataa
     3241 tccatagaga caagtcactt agtccctttc tactagagag tgcgtggttg ctggaaacgc
     3301 ataggaagtc taaactgata ctactcttga gttttttatg aaaacataaa acggtggcca
     3361 cgttagagcc aatcagaggt gtccctctct tttgaggtac ataaatgaag gtggaaccac
     3421 gttgcgacgt cctttcgagg atgtcgcatt tttttattag gatactaatt atggagttgc
     3481 aagaattagt ggagcgcagt tgggcaatcc gacaagctta tcacgaactg gaagttaagc
     3541 atcatgattc caagtggacg gtagaagaag acctcttggc tttatctaat gatattggaa
     3601 atttccaacg actggtgatg acaaagcaag gacgctacta tgatgaaaca ccctacacac
     3661 tggaacaaaa actttcaaaa aatatctggt ggctattaga actttctcaa cgtttggata
     3721 tagacattct gacggaaatg gaaaacttcc tctctgataa agaaaagcaa ttgaacgtta
     3781 ggacttggaa gtagtctgct gataaaaaat caatgcttag aaactatgaa ataataaaaa
     3841 aggagaacat catgattaac attactttcc cagatggcgc tgttcgtgaa ttcgaatctg
     3901 gcgtaacaac ttttgaaatt gcccaatcta tcagcaattc cctagctaaa aaagccttgg
     3961 ctggtaaatt caacggcaaa ctcatcgaca ctactcgcgc tatcactgaa gatggaagca
     4021 tcgaaattgt gacacctgat cacgaagatg cccttccaat cttgcgtcac tcagcagctc
     4081 acttgttcgc ccaagcagct cgtcgtcttt tcccagacat tcacttggga gttggtccag
     4141 ccatcgaaga tggtttctac tacgatactg acaacacagc tggtcaaatc tctaacgaag
     4201 accttcctcg tatcgaagaa gaaatgcaaa aaatcgtcaa agaaaacttc ccatctattc
     4261 gtgaagaagt gactaaagac gaggcacgtg aaatcttcaa aaatgaccct tacaagttgg
     4321 aattgattga agaacactca gaagacgaag gcggtttgac tatctatcgt cagggtgaat
     4381 atgtagacct ctgccgtgga cctcacgttc catcaacagg tcgtatccaa atcttccacc
     4441 ttctccatgt agctggtgcg tactggcgtg gaaacagcga caacgctatg atgcaacgta
     4501 tctacggtac agcttggttt gacaagaaag acttgaaaaa ctaccttcaa atgcgtgaag
     4561 aagctaagga acgtgaccac cgtaaacttg gtaaagagct tgacctcttt atgatttcac
     4621 aagaagtggg acaaggtttg ccattctggt tgccaaatgg tgcgactatc cgtcgtgaat
     4681 tggaacgcta catcgtaaac aaagagttgg cttctggcta ccaacacgtc tacactccac
     4741 cacttgcttc tgttgagctt tacaagactt ctggtcactg ggatcattac caagaagaca
     4801 tgttcccaac catggacatg ggtgacgggg aagaatttgt ccttcgtcca atgaactgtc
     4861 cgcaccacat ccaagttttc aaacaccatg ttcactctta ccgtgaattg ccaatccgta
     4921 tcgctgaaat cggtatgatg caccgttacg aaaaatctgg tgccctcact ggccttcaac
     4981 gtgtacgtga aatgtcactc aacgacggtc acctattcgt tactccagaa caaatccaag
     5041 aagaattcca acgtgccctt cagttgatta tcgatgttta tgaagacttc aacttgactg
     5101 actaccgctt ccgcctctct cttcgtgacc ctcaagatac tcataagtac tttgataacg
     5161 atgagatgtg ggaaaatgcc caaaccatgc ttcgtgcagc tcttgatgaa atgggcgtga
     5221 actactttga agccgaaggt gaagcagcct tctacggacc aaaattggat atccagatta
     5281 aaactgccct tggaaaagaa gaaacccttt ctactatcca acttgatttc ttgttgccag
     5341 aacgcttcga cctcaaatac atcggagctg atggcgaaga tcaccgtcca gtcatgatcc
     5401 accgtggggt tatctcaact atggaacgct tcacagctat cttgattgag aactacaagg
     5461 gggccttccc aacatggctg gcaccacacc aagtaaccct catcccagta tctaacgaaa
     5521 aacacgtgga ctacgcttgg gaagtggcca agaaactccg tgaccgcggt gtccgtgcag
     5581 acgtagatga gcgcaatgaa aaaatgcagt tcaagattcg tgcttcacaa accagcaaga
     5641 ttccttacca attaattgtt ggagacaaag aaatggaaga cgaaacagtc aacgttcgtc
     5701 gctacggcca aaaagaaaca caaactgtct cagttgataa ttttgttcaa gctatcctag
     5761 ctgatatcgc caacaaatca cgcgttgaga aataagagtc tagcataaaa gcctccaatc
     5821 tggaggcttt ttctcatcta tttttactca aggactaagt tcacttgagc aaactgaatc
     5881 cgcactgtcg ttccttttcc gacctcagac tcgatacgaa tctggtgccc cagttcttca
     5941 gaaattttct tagatagata aaggccaagt ccagaggact gctgggtcaa acggccattg
     6001 tatcctgaaa agccacgttc aaatactcgg aggacatcac tgttttttat cccgattccc
     6061 gtatctttga tacaaagctc ttggtcatcc atataaatct ccagaccacc ttccttggtg
     6121 tacttgagac tgtttgagat gatttgctca ataaccacta gcagccactt tttatccgtc
     6181 acgatttctt tatcaaggtc atgtagattg acatttaagc ctttttgaat aaagacaaga
     6241 gcatatttac gaattatttc cttgaccaag tcctcaattt gaacctgctt taagaccaaa
     6301 tcatcatgga aactttctaa acgcaggtac tgtaaaacta ggttggtata ggagtcgatt
     6361 ttgaaaattt cctgttctag ttgctgcttc agttggcggt cgaccacttc tgcaactaag
     6421 agttgactgg ctgcaatggg ggtctttatc tgatggaccc acaaggtata gtaatccagc
     6481 aaatccgtca gttttctttc tgcttttgac ctctgctgat agagttccat ctcacgcgct
     6541 tctaattttt ctgctaaagc tatttccaaa ggagacttgg cttccctctc tccataaaga
     6601 agttcctggc gatagacctg cgtttccacc aatatatccc aagtgaaaaa taagatggtt
     6661 acaaagcaac acaagaagaa aaagtagagg aagtaaattc ctagactggc aaataaaaac
     6721 tgaaagagta agaccagaaa tgccaaagaa agcagataga taaaaagacg actacgggag
     6781 cgcagatagg ctagaaaaaa ttgtttccaa tcaagcatgc ttcaatccgt accctattcc
     6841 tttcttggtc tcgataaatc ctaccaatcc ctgctcctcc aactttttac gcaaacgagc
     6901 cacattgaca gagagggtat tatcatcaat gaaaaagtca ctgttccaaa gttcccgcat
     6961 caggtcgtca cgtgctacga tgttgcctgc atgctcaaat aacacgcgta aaatctggaa
     7021 ttcattcttg gtcaaattca agacttgccc ttgataatgt aaatccatgg atttggtatt
     7081 gaggataaca ccagcatatt ccagcaaact ctcatcacgc ccaaactcat aggaacgacg
     7141 caacaagccc tgaaccttag ctaaaagaac ctgctggtca aaaggcttgg tcacaaagtc
     7201 atccgccccc atattgattg ccatgacaat atccatagcc tggtctctcg aagaaagaaa
     7261 catgataggt accttggaaa tcttgcggat ttcctgacac cagtgataac cattaaacaa
     7321 gggcaaacca atatccatga ggaccagatg aggttccgac tgaacaaata gactcaaaac
     7381 ttccataaag tcttctacca ggaccacttc aactccccat tcagagagca ttttcccgac
     7441 ctgttgacga atgacctgat catcttccac taataaaatc ttgtgcatgc gcttctcctt
     7501 ttccattatt ataacagatt tttccatgcc agatggtctg aaactgaatt tgaaatagcc
     7561 tgtttttagc cagtacaaac aggcaatgct actagctaat ttgagggaaa tttgctaaga
     7621 taaataaaaa gaaaggagct cttatggcca atatttttga ctatctgaaa gatgtcgcat
     7681 atgattctta ttacgacctt cccttgaatg agttagacat tctaacctta atagaaatca
     7741 cctacctctc ctttgataat ctggtctcca cacttcctca acgtctttta gatctagcac
     7801 ctcaggttcc aagagatccc accatgctta ctagcaaaaa tcgccttcaa ttattagatg
     7861 aattggctca acacaagcgc ttcaaaaatt gcaaactctc ccattttatc aacgacatcg
     7921 accctgaact gcaaaagcaa tttgcggcta tgacttatcg tgtcagcctc gatacctatc
     7981 tgattgtctt tcgtgggaca gatgacagta tcattggctg gaaggaagat ttccacctga
     8041 cctatatgaa ggaaattcct gctcaaaagc acgcccttcg ctatttaaag aacttttttg
     8101 cccatcatcc taagcaaaag gttattctgg ctgggcattc caagggagga aatctcgcta
     8161 tctatgctgc tagccaaatt gagcaaagtt tgcaaaatca gatcacagca gtttatacat
     8221 ttgatgcacc tggtctccat caagaattga cacagactgc gggttatcaa aggataatgg
     8281 atagaagcaa gatattcatt ccacaaggtt ccattatcgg tatgatgctg gaaattcctg
     8341 ctcaccaaat catcgttcag agtactgccc tgggtggcat cgcccagcac gataccttta
     8401 gttggcagat tgaggacaag cacttcgttc aactggataa gaccaacagt gatagccggc
     8461 aagtagacac aacctttaaa gaatgggtgg ccacagtccc tgacgaagaa cttcagctct
     8521 acttcgacct cttctttggc actattcttg atgctggtat tagctctatc aatgacttgg
     8581 cttccttaaa ggcgcttgaa tacattcatc atctctttgt ccaagctcaa tccctcactc
     8641 cagaagaaag agaaaccttg ggtcgcctta cccagttatt gattgatact cgttaccagg
     8701 catggaaaaa tagataatac tcttgaaaat taaatgtata caaaacaaaa gacctagaat
     8761 acatactttc atgtgcattc taagtctttt taaatagaat ctaatagtca ataaaaatca
     8821 aagagcattg agagataatg gggcttggaa cgtccctctc gtttcaacaa aatgacccca
     8881 ttatagatta aaaagatgcc acttagaaaa agcaaaaaag gaagtaagac aaaggcaaat
     8941 acataaaaag ctaactgaac attctcgtat ccatttttat aaaaaaggta ggatagataa
     9001 aaataacttg aaatgaggga taataaaaat aatactggat tccacaaact tctattatcc
     9061 ttccaaaatg acactataaa ggctaataca atttctataa cgagatacat ttcttactcc
     9121 tttaatagct acattttatc ataattatcc aaagaaaaaa agagggcatt tatccctctt
     9181 aatccttcat ctgactctct gcatcggcca cgactttttc tagactggtc tgaccaagtt
     9241 ctgcctccat agtcaactga attctctcca atttttgatc caaaacatca tgaatatgag
     9301 ctcctacagg gcaatttgga ttcggattgt catggaaact gaagagttga cctgtcttac
     9361 caagacattc gaccgcctga taaacatcta aaagactaat atccttaagg tccttgacaa
     9421 tctctgttcc gcccgttcca cgcgctactg aaatcagctc tgccttcttc aactgggaca
     9481 agatctttct gataatgaca ggattgaccc caacactagc agccagaaaa tcactggtca
     9541 ccttgctttc cttcccctcg agggcaatga ttatcagcat atgagttgca atggtaaatc
     9601 tacttggaat ttgcatcctc ttctcctttt tacgaggcta ccctgcctct actcttcttt
     9661 ttctattatt ataccctttt tagttgtaat gtcaatcgtt accacttttc aaccagtcgt
     9721 ctaactcccg atcgcaaccc tctttctgag ccaattctct caaaaattcc tgatgatgag
     9781 tatggtggat cccattgacc agactttcat agtaaacctc aaaataggga agtctcaggt
     9841 ctttagccag ctgcaattca gctgccacat cgtaatctac ccgtcggaag tccatatcta
     9901 ccaggccctt gtcatcaaac tccaaaatca tatactgggc ccgcaagtcc ttccgtagct
     9961 gagcgtccaa aaagaaaggt tggccaatcg aacccggatt gacaattaat tgcccaccag
    10021 tcccgtaacg aagcaactgc tggtgaatat gtccataaac agcaatatca cagggaggat
    10081 gagtcaccaa gcggtcaaac tcctcttgtt tgccagtatg aatcaactct cgcccccagt
    10141 tcttatcagg cagatgatgg ctaattccca ccgtcaaatc cccaaactga cgatgaattt
    10201 gaagaggttg attgtggagc acttcaattt cttctaggga aatttcctct aaaacatact
    10261 ggcactggcg caagagatag cgttgactgg ggcgagtact gtccaattcc ttacggacac
    10321 catgccaaag actgtcttcc cagtttccca aaactctagc cgtaatcggt agttgatcca
    10381 acaagtccaa aatccttcta cgccctgtcc ctggcatgag aatatctccc aaaagccagt
    10441 attcatccac tcctagctgc cgagcatctg ccaaaacagc ctccaaggcg gtggtatttc
    10501 catgaatatc tgaaagaaga gctattttcg tcatatccat ctcctcgttt tttctcttgc
    10561 aataagtata acataaaaag tcacagctag agaaatctag ctttttttga tatactagat
    10621 aaagatatta gacaagagga aacgaatgac cccaaacaaa gaagactatc taaaatgtat
    10681 ttatgaaatt ggcatagacc tgcataagat taccaacaag gaaattgcgg ctcgcatgca
    10741 agtctctccc cctgccgtaa ctgaaatgat caaacgaatg aaaagtgaaa atctcatcct
    10801 aaaggacaag gaatgtggct atctactgac tgacctcggt ctcaaactgg tctctgagct
    10861 ctatcgtaag caccgcttga ttgaagtttt tctagttcat catttagact atacaagtga
    10921 ccagattcac gaggaagctg aggtcttgga acacactgtc tctgacctgt tcgtggaaag
    10981 actagataaa ctgctaggtt tccctaaaac ctgcccccac ggaggaacta ttcctgccaa
    11041 gggagaactc ctcgttgaaa tcaataacct cccactagct gatatcaagg aagctggcgc
    11101 ctaccgcctg actcgggtgc acgatagttt tgacattctc cattatctgg acaagcactc
    11161 acttcacatc ggtgaccagc tccaagtcaa gcagtttgat ggcttcagca ataccttcac
    11221 tatcctcagt aacgacgagg atttacaagt gaatatggac attgcaaaac aactctatgt
    11281 cgagaaaatc aactaatttc tcaagtcccc taccaaccct gaaagtttta ttttcagggt
    11341 tttatttcta tcattttatt ttactttcat ttggtggtag aatatttgaa aaaaagaaag
    11401 gtagtttaac ctatgaaaaa atcactaaaa atttttgcta catctaaatg atttgacctc
    11461 ttcggggttg ctttggtcgt tgggattgcg attgcatctg gttatctcaa ctcacgcctc
    11521 gataaatttg tagactgggg actatggaca gctcttgtac cctttggatt gatttccgta
    11581 accaacgttg ggatttccat gttgtccact cgtttcacgg ggaaattaag caaatgggga
    11641 aattactttg gtattgttaa taccattttg tccggtgcta ttgattatat ccttggaaat
    11701 aaggcggcca tcattaccta tcccgtcacc ttcctcattt atacctttgc tataaagaaa
    11761 tggaaagctt cgcaagaagg cagacccaac caaatgagcc aaaaacaggt aaaattggcg
    11821 gccatcatca tttccatcat cgccttcctc tttgcctttg tgaccaacta tatcggatat
    11881 gggggcaaga tgaatctcct tgcctacgta acaactattg cctttgcact gtccctcatt
    11941 gccaatgctt tgaatgcatt ggcaatgagg gacagtgggg cttttggttg atttacaatt
    12001 tcgttcagct gacaaaagct ggtattcaag gaaacttcgc caatatcggt aaatacatct
    12061 tttatatcct caatgcaatc ggagctttat ttgtctggaa tgatgaagaa gttagataat
    12121 agaaggaaac tcaaataaag aatactagcc aacttaagag aatctcccct ctgctatttt
    12181 tatcatttta gttgtaactc ccattctttt agagtagaat gaagttagat aaaagaggga
    12241 ggtcttgtca tgaaaaaact ctatagaatc cattttatag caattgcagt tattgatttg
    12301 ctactatttg cattttttat cattagactc gagacatctt ttgagtggct cttgctctct
    12361 ggtttaattt tcttccttgc tcaaggactc ctgctatttc tcttgatcgt ccgactcaaa
    12421 cattaattcg ctgagattta tcctcaaatc aataaaaaga ttcgcttcta ctatttaggg
    12481 gttctcacca ttgattttct attttttgtt ctcttagcct tcattagttc tcagcgtttt
    12541 tcatctctta tgtcaattat cactgcttgc cattctactt tttattatat gacagctgac
    12601 tacctaagag aaaactatcc agacttttac gacaaacaca tctctttatg ggagtgtctc
    12661 taaggaaaag gaggttttag catgaaaaaa atcatcttca tcaaaaccat tcaactcctt
    12721 gtcattgatg gaatcatgct ggcatttttg acatttaaaa aggggggctt acttgggact
    12781 ggattttgat ttatagcggt tggctcattt tctttcatcc tgtgctattg acctatcttt
    12841 caaaccaact ttgtgaccac tttagttaac tctattccca gattagaccg agattctggc
    12901 gttttgcttt acaaattctc ctatgggata gcctgatgat tctctccttg gtgtctttaa
    12961 gtgatattcc acttttcctt cagggaactc tcctcatcct aggacatctc atcccttcct
    13021 atcgcatctg ccaaagcctg aaaagagact tcccccaagc atatcaagaa ccgatttctt
    13081 tttggagtat tttatgatcg atgagaaaga ccaagccgac tgggcttggt ctttcttatc
    13141 tctttttagt atctagaata atggtaacag gtccgttatt aaccagctca acctgcatat
    13201 ctgctccaaa gatacctgtc tgaacgggca cttcttgcgc taatttttga ttgaaagcat
    13261 catagaagtc tgatgccata tcaggtttag ctacccctgt aaaggctgga cgattgcctt
    13321 tcttagtatc cgcaaagagg gtaaactgag aaatagagag gatttctcct tcaatatctt
    13381 tgacagatag gttcatcttg ccttctgcgt ctgaaaaaat ccgcatattg accagttttc
    13441 tcacagcata gtccaaatct tcctcttggt cctctggtcc aacaccaacc agcaataaaa
    13501 gtccctgatt gatttttccc tgaatctggc cttctatact cacttgggct tttttaaccc
    13561 gttggataat gattttcata atagcctttc tagtaagagc taggacaact agccgttggt
    13621 ccgtttgaca gagtaaactt ctggcacact cttaatttta tcgacaaccg tagtcagtgt
    13681 agagaggttg gcaataccga aggacacatg gatattagca aacttcatat ccttggttgg
    13741 ttgggcattg accgttgaaa tattcttggt tgtatttgaa agaacttgca gtacatcgtt
    13801 caacagtcct gtacggttga gaccgtagat atcgatatgg gccatatact ccttatttga
    13861 gctagagtac tggtcttccc attccacatc aaggagacgt tgctcgtagt tttcttgggc
    13921 acgcaggttc atacagtcca cacggtgaat agccacacca cgacccttgg taatgtagcc
    13981 aacaatatcg tcaccaggca cggggttaca acacttagca atccgcacta ggagaccaga
    14041 agcaccttca ataaccactc ccccctcatg cttgaccttg agggtttctt tattttcaac
    14101 cttgacctcg ccacctttga caagctcctc tgcctcagcc ttggccttgg cacgctcttc
    14161 ctcacggcgt tccttttcag tcagacggtt aaagacggta atcgcaccga tttccccaaa
    14221 accaatggcc gcaaagaggg agtcttctgt cttgtaactg gtcttttgca gaacttgatc
    14281 catgtggcgc ttgtccataa atttatttgc cacatagcca ttttcttgga actgagccat
    14341 cagcatctca cgacccttgt tgacagacaa ttccttatct tggtttttaa agaactggcg
    14401 aatcttattg cgcgccttgc tagtcttgac catattgagc cagtcacggc taggtccaaa
    14461 ggagttcggg ttggcgataa tttcaacctg atcccctgtc tttaacttgg ttgtcagtgg
    14521 aaccatgcgg ccattgacct tggcaccagt tgctttttca ccgaccttgg tatggatttc
    14581 gtaggcaaaa tcaatcggtc ctgaatcttt gggaagggaa cggacagctc catctggggt
    14641 aaaaacgtaa atctcctcag ccaaatagtt ttccttaaca gagtccacaa attccttagc
    14701 atcatcagcc tggtcttgga gctccatcat ctccttgatc cagttcattc caatagctga
    14761 ttccttgctg ttaacttgcc cctttatacc tttcttataa gcccagtgag ccgcaacccc
    14821 gtactcagcc acctcgtgca tttccttggt tcgaatctgg aattcaatcg gcccttttgg
    14881 tccataaaca gtcgtatgga tagactgata accattggcc ttgcggttgg cgatatagtc
    14941 tttgaagcga cctggcatcg gtttccaaaa ttcatgcacg taaccaagca tggcataaac
    15001 atcactttgg gtatctaaaa tacaacgaat agcaatcaga tcatagattt cctcaaaccg
    15061 ttttctcttg tcctgcattt tgcggaaaat tgagtaaata tgcttgggac gaccataaat
    15121 cttccctttc aagtgacgtt ctgtcgtata ctcctctaat tttgtgacta cctcatccac
    15181 caaggcctca cgctccctgc gcttttcctt catcatatgg gtaatcttgt aaaactccgt
    15241 tggattgaga taacggaaag acaagtcttc taattcccat ttgacactgg aaatccccaa
    15301 acgatgggca agtggggcat agatttccat ggtttctttg gaaatacgct cctgcttgtc
    15361 ttttcgaaga tgtttcaggg tccgcatatt gtgcaagcgg tcagacagtt tgaccaaaat
    15421 aacgcggatg tcctcagaca tggccatgag catcttgcga tgattttccg ctaattgctc
    15481 ctcgatcgat ttgtactcga ccttgccaag cttggtaact ccgtcaacaa tcatccgcac
    15541 atcaggacca aactctcttt ccaaatcgtc caaagtcgca tctgtatctt ccaccacatc
    15601 atgcaagaat ccacaagcta ctgttacagc atccagcttt agcttagcta aaatacctgc
    15661 cacttggata gggtgaatga tataaggctc gcctgatttg cgatattgac cactgtggca
    15721 ttcaacagca tagaccaagg ccttatggac aaaatgaaca tcctcttccg ttaaatattc
    15781 tttggttaaa gcgacaactt cttcgcctgt taaattcact tctttcggca tctctactct
    15841 ccaattcttc ctaccatttt atcacttttt taagaatatg aaaactagat tggaacagaa
    15901 taagaaaaaa ataattcaaa attgcttgat aattctgaat tattggcccg taatatacta
    15961 cgaagttaga ttttaaactt aggtgataga aggagagata gaagaacgga aaccatattg
    16021 taacccaaag actttctgac ttccccaatt ccattgaaga tacgaaagat aaacggtgga
    16081 actcgtatca catacactgg taccttgact ggattttgga attaatacta aatgaaaatc
    16141 aaagagcaaa ctaggaaact agccgcaggt tactcaaagc accgctttga ggttgtagat
    16201 aagactgaca aagtcagtca catatatacg gcaaggcgac gttgacgcgg tttgaagaga
    16261 tttttgaaga gtataaaaat cctcaagata ctttcttcta tcctttagtt tataaggaga
    16321 atacctatga aaaaactgct atttctatct ttgctctcct aacgttagga gtttgctgcc
    16381 tgttcttatt cagccagcaa agctataaaa aacagtcgtt caatactatg ctaacgacca
    16441 gaacctgccc agtaggataa cttatagtga gtatagcgac aaatgtgaag ccaactacgg
    16501 tagcactcta aacatcacgt ctatcaaaca agctaatgac ggagtttatg caacctatga
    16561 agggcaattg acacctttcc aatattgata aattgataac cagcctgtct tcatctagtc
    16621 atgctggttt ttaagttcat tttaaatcct tacctattct ccctaactgt gctatactta
    16681 atttatactc aatgaaaatc aaagagcaaa ctagaaagct agccgcaggc tgttcaaagc
    16741 actgctttga ggttgcagat aaaattgacg cagtttgaag agattttcga agagtattag
    16801 tacattcttt gagattggag ctagtatgaa aatccataaa accgtgaatc ctgttgccta
    16861 tgaaaatacc tattatctag aaggcgaaaa gcacctcatc gtcgtcgatc ctggtagtca
    16921 ttgggaagcc attcgtcaga caatcgagaa gatcaacaaa ccgatctgtg ctattctctt
    16981 gacccacgcc cattatgacc atatcatgag tctggacttg gtttgcgaga cgtttggcaa
    17041 tcctcctgtc tatatcgcag agagcgaagc cagctggctc tacactcctg tcgataatct
    17101 ctccggtctc cctcgccacg atgatatggc agatgtggtc acaaaacctg cagaacacac
    17161 ctttgtcttt cacgaagaat accaactaga ggaatttcat tttaaggttc tgccgacccc
    17221 agggcactct atcggtggtg tttccctagt ctttcctgat gctcacctag tcttgacggg
    17281 agatgctcta ttccgcgaaa ctatcggacg gaccgacctt ccgactggta gcatggagca
    17341 actccttcat agtatccaga cccaactctt caccctatca aactacgatg tctatccagg
    17401 acatggtcca gctactacta tcgctcacga aaaggccttc aatccctttt tctagcaaga
    17461 tgatgacaat cgaaatttaa gtaaactatc cagcaaatct ttctattaca aaaggcggct
    17521 ctataatatt tgtagtgggt aaatccacta tagatattat ggagcctatt ttattgtaga
    17581 aaaaaaatcc cataagatct ataatgaaaa gcgacaaaac c
//
LOCUS       DAVKZB010000031        17226 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKZB010000031 DAVKZB010000000
VERSION     DAVKZB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.031"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(393..2120)
                     /gene="ezrA"
                     /locus_tag="VXA80_000950"
     CDS             complement(393..2120)
                     /gene="ezrA"
                     /locus_tag="VXA80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV0353467.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2202..4148)
                     /gene="gyrB"
                     /locus_tag="VXA80_000951"
     CDS             complement(2202..4148)
                     /gene="gyrB"
                     /locus_tag="VXA80_000951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV0353468.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4163..4726)
                     /locus_tag="VXA80_000952"
     CDS             complement(4163..4726)
                     /locus_tag="VXA80_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0353469.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQT
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4811..5365)
                     /locus_tag="VXA80_000953"
     CDS             complement(4811..5365)
                     /locus_tag="VXA80_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV0353470.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDDVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5376..6599)
                     /locus_tag="VXA80_000954"
     CDS             complement(5376..6599)
                     /locus_tag="VXA80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0353471.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6636..9086)
                     /locus_tag="VXA80_000955"
     CDS             complement(6636..9086)
                     /locus_tag="VXA80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV0353472.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9285..10214)
                     /locus_tag="VXA80_000956"
     CDS             complement(9285..10214)
                     /locus_tag="VXA80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0353473.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10354..10641
                     /locus_tag="VXA80_000957"
     CDS             10354..10641
                     /locus_tag="VXA80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV0353474.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10675..12009)
                     /gene="ciaH"
                     /locus_tag="VXA80_000958"
     CDS             complement(10675..12009)
                     /gene="ciaH"
                     /locus_tag="VXA80_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV0353475.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11999..12673)
                     /gene="ciaR"
                     /locus_tag="VXA80_000959"
     CDS             complement(11999..12673)
                     /gene="ciaR"
                     /locus_tag="VXA80_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV0353476.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12782..15328)
                     /locus_tag="VXA80_000960"
     CDS             complement(12782..15328)
                     /locus_tag="VXA80_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV0353477.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLETVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15451..15891)
                     /locus_tag="VXA80_000961"
     CDS             complement(15451..15891)
                     /locus_tag="VXA80_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV0353478.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15866..16735)
                     /locus_tag="VXA80_000962"
     CDS             complement(15866..16735)
                     /locus_tag="VXA80_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV0353479.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16777..>17226)
                     /locus_tag="VXA80_000963"
     CDS             complement(16777..>17226)
                     /locus_tag="VXA80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353480.1"
                     /translation="ANFQVEFLNNLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGD
                     FESYQDFVLKGGFGFVILKNNELIAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGA
                     AMILESLNRDMFPLWDAHNEASKKVAEFLGYELSEPYEAFELEEILI"
BASE COUNT         5200 a         3710 c         3111 g         5205 t
ORIGIN      
        1 tacaagaaaa gtgcaaataa gaaatctcca gattaggaac tatccgtgag ttctctagtc
       61 tggagatttt tcaatagact tcgttattgg acggttacca agttactcaa agtacagatt
      121 tgaagctgca gataagactg acgaaatcac tttaatacta aatgaaaatc aaaaagcaaa
      181 ctaagaagct agcctcaggc tgtacttgag tacggtaagg cgacgctgac gtgatttgaa
      241 gagattttcg aagagtataa aacactgttt tgaggtggtg gataaatcgg ctgggtaaaa
      301 actcaatctc aggagaaaat gaagtaaatc tccccacaac tatagaaaaa gatttgattc
      361 tgctcctcac acaataaaat ctttttcttt tattaaaaac gaatcgtttc acgtgttttc
      421 tcatatgagg taacaaagcg attggttaca ccaggctctg ccacttccaa tgcttgagaa
      481 atcttgtcaa atgaagcgtg ataatcaaat tctttttcaa aaatatctaa agcttcgtta
      541 aatgcttctt gaatgcgttc atcaaatgag cgatagcggt tagaatattg caagagttgc
      601 tctgtcaaag ttgcatattg tacaatatta taagtttccg tttctaaagc ttccatatca
      661 ttcgttgcaa tttcaagaac tcgggtaaca gattcaatgt taatcatttt ttgttctaac
      721 tcaaccatta aatcctcggt attattgctt gccgtaaaga ataacttcaa gaaagtttgt
      781 ggaatacctg gcagattgcg tttttccatg tatcgcttga tagtatggag acgattgaca
      841 taaacattgg ccttttgacg tgcattaata tcatctttct caatttgtgt caggcgctca
      901 ctaactgaaa tttgctcatc ttcaatatct tttagttgag tttgtaaatc ctcaagattt
      961 tcttcaagaa ctgaataagc ttgggttggt tcttcttgat ttgaagttac ctcaacaata
     1021 gctgcctcaa aactctctaa ttctgtctga atacgacgaa catggcttgc agctgtctca
     1081 ggaagtaaat aggtcttgtt caaacgtgca atatcttctc ccaataaagt attattctct
     1141 ttcatatgtt gaaggtaagt tggaagagtt gcaagtagat tttccactac tttctgagca
     1201 gcaatttctc gagtaaaaat atcatacaag gcattgattt cctcttgtgc ctgtccattc
     1261 tcatattcgg cattatccaa ttccaactga cgaatattct cttggttttt cttgaatgct
     1321 tcataaagca agtggaaacg cgcttcaata tccgtttcaa caaaatgata attagcatca
     1381 attagtttac gataaccgga ttccaaatcc tgtaattgat ctggcaattc tgtagaaagc
     1441 gtcgtaacca aggctggaac acgatccaca atatgactta aggccaaaat gtgattttct
     1501 gtattatcca aaatcactgc ggcttccaca gggtcacccg atgaattcaa ggttacaaat
     1561 tgtgaaaatt cagattggat attttctaat tgtttttcaa tttcatccaa ggcttgacca
     1621 tactgttctg aattttcagc aactctatgc tgaagttcct caaataaatc caaagcatga
     1681 agaacacgac cactattttt agattcttgc ttctctaagt ctgccaaagc attgcgaatt
     1741 gccgcaatat cttcttcaat caaagtaatt tgactctcaa tttggtcaat ttgatgactg
     1801 gccttgagaa aacgaaatga atggttataa ccttctgctt caaagagatt attttcaata
     1861 tcggcaaaag agttgagaga taaatcgacc catttttgat tccattcacg gaaagccact
     1921 tgactttgtc caatcaagtg catatttttt acagcttcta cttcatcatt tactggaaga
     1981 ttgtatagtt cttcttttct ttcttctagc gcctctaatc tcccctcgtt tcgcttacgt
     2041 agaaagattg ccactacata agccagaact aaaatgactg caattgcaac cattaaataa
     2101 attagttgtc cattagacat atcaaactcc ttttttactt gaaacaatcg taacgattat
     2161 atcatatttt ttagaccaag ggaactactt ctccctattt tttagacatc aagtgtacta
     2221 tagacagcat tttcttcgat aaactcacga cgaggctcta ctcgatcccc catcaacata
     2281 tcaaagattt tatctgcttc tgcagcatca tctacagaaa ctctagccat caagcgatgt
     2341 tcgggatcca tggttgtttc ccacagctga tgatcgtcca tttcacctag ccccttataa
     2401 cgctgaatag tcggtttggt acgaccttca ctataacggg ctaaagcttc ttggagtttg
     2461 atttcttgat ctgcacccgg ctggatatat tctttaatct cgctcccaac cttgacacca
     2521 tagattggtg gttgggcaat ataaacataa ccagcttcta ggattggttt catataacga
     2581 taaatcaagg ttaaaagaag ggtacgaata tgggctccat cgacatcggc atcggtcatc
     2641 aaaacgagtt tttggtaacg ggctttcgaa acatcaaatt ctgcgccaaa tcctgttccc
     2701 atggctgtga aaagactacg aatttcttcg ttagctagaa tcttatccat acttgctttt
     2761 tcaacgttca aaatcttacc gcgaattgga aggatagcct gaaactcacg gttacgacca
     2821 gatttggctg atccaccagc tgagtctcct tcgacgatga agagttctgt ttcagcaggg
     2881 ttattagaag aacagtctgc tagtttccct ggaaggttgg aaatttccaa accagatttt
     2941 ttacgtgtga cttcacgcgc acgcttggca gccacacgag ccttggcagc taaaatccct
     3001 ttttccacga tacgtttggc aatctgtgga ttttccatga ggaaatcgga gaaggcttca
     3061 ctgaagaggc gattggtaat cttgaccact tcgctatttc ccaatttggt cttggtttgt
     3121 ccttcaaact gtggatttgg gtgtttaact gagataactg cagttaatcc ttcgcgaaca
     3181 tcttcccctg ttaaattatc ttcattgtct ttcagtaact tattcttacg agcataatca
     3241 ttgataacac gagtcagtgc cgtacggaaa ccttgttcat gcgttccacc ttcatgggta
     3301 tgaatattat tggcgaaact catgacattt tcatggtaac ctgttgtgta ctgcatggct
     3361 acctcaactg taatatcatc catctcaccg tctgtataga ttggtgtatc aaagattaca
     3421 tccttgttct cgttgatata ttcaacgtaa ctagcaatcc caccttcata atgataatgc
     3481 ttggtttgtt ccaaaccttg gcgcttatca gtgatagaaa tttgaagacc gcgatttaga
     3541 aaggccaact cttgaatccg tttatttaat ttatcaaaat caaaggttgt tgtttcagta
     3601 aagatttttg ggtccggtat aaagtgaacc gttgttccag ttctatctgt atccccaacc
     3661 acctcaaggt ctgcaacaac atgaccacga cggtattctt ggtaatgaat cttaccgttt
     3721 ttatggacat gaacgtctaa ttgagtggaa agggcattaa ctactgacga ccccaccccg
     3781 tgaagaccac ctgaaacctt gtatccacca ccgccgaact ttcctccagc atgaaggact
     3841 gtaaagacgg tctcaacagc aggacggcct gttttttcct gaatatcgac tgggatacca
     3901 cgcccatcat ccacaacagt aatcgaatca tctggctcaa taaaaacttg aatatggctg
     3961 gcaaatcctg ccaaggcctc gtcaattgag ttatcaacaa tttcccagac tagatggtga
     4021 agaccttctt ttgaggttga tccaatgtac atccctggac gcatacgaac agcctctaag
     4081 ccctctaaaa cttgaatttg actggcatca taatcctgtg cctgcagatt tttgatttct
     4141 tctgtcatct tattcctttt tcttatatgt ctaatacttg tcttaaattc acgatactgg
     4201 taaacaagtg ggtatcaagt cctgcagctt gacctgcttc gatatcaatc ggccgatcac
     4261 caatgacaag accagagcta atctgatact tttctcttaa ataaagcatg gattcgggat
     4321 ttggctttct cttaaagcct gagctagaag tcaccacttc tgtaaaataa gctgctatag
     4381 aggttttttc taaaatttcc aaaacctgat catttcgatg agagaccaaa aaatgacggc
     4441 caccttgatt taaaatgtct tccaataggt cagaaactcc ttcaaataaa atcgggtgtt
     4501 caagctctct ggcttcattt tccttgtact tttctaaaaa attctctaaa ttgggagcga
     4561 atgtctcaat cgcaaaagga gtagaaacct ttaaagtttg atagacactg tcatggtctt
     4621 gtgtgatacc atacagtgcc aatgtttcaa caaatgcagc tgttgaagtt tcataattat
     4681 ccagtaaagt tccacctaaa tcccagatgt aatcgtgata tttcataccc ttcattatag
     4741 cattttttta tgaaaaaagc gatgatttcc ctgagttttc cacataaaaa acgagagaat
     4801 aacccccgtt ttactgattt ttaccaaaga catctcgata gagcattcct gttcgtaaac
     4861 tctctgcgtc ccctcctagt tgctccacca actcgtaggc aaaggcaagg gctgttgaag
     4921 gaccccgact ggttgtcaac tgaccatcta ccactactgt ttccttgacg tagtgaccat
     4981 caaggatttg ctcttgaacg tcgtcataac aagtgtatcg cttatttttc aatatctctg
     5041 cttgattgag ggcaattggt gccgcacaaa tggctgctag tttcttccct tcttgctcga
     5101 agctttgcaa ttcttgaatc aaggtctgat tatcacgtaa atgtgcagaa ccaggcatac
     5161 ctccaggaag aacaatcata tcatagtctg ataaatctcc atcaaagaca tgatctgctc
     5221 ttacttggat tgcatgcgaa cccgttactt gctcttcaaa accaaccata tcacatgtga
     5281 tattggctcg acgcaagaca tctacaactg tcaaggcttc aatttcttca aagccctgag
     5341 ctaacataac tgctactttt accatgattt tctccttatt taatttgttt taatacaacc
     5401 tttttccgtt tgaatgggac ttttccgctc ttttcttcag ctagattagt ctggtaactc
     5461 atcgataaaa gcaaaccaac accaatcaga ttactgataa tagctgatcc cccttgcgaa
     5521 atgaaaggca agggaatccc cgtcaaagga agtagtccag tcacagcacc gatattctca
     5581 aagatgtgga agagcaacat cataatcaaa cctgtggaaa tataagtgta gaactggtta
     5641 tttgatttaa gagtaatctt caacatacgg taaatcaaca tgagatagag ggcaataacc
     5701 aggacagagc caataaagcc aaaatcttct gcaataaccg taaaaatcat atctgactct
     5761 cgaactggga taagcagatt cgaagcatta aatccctgac caaataagcc accactccca
     5821 atggcaatct gcccttgagc ctgctggtaa gtcgttgttt gggcaaactc aaagggattg
     5881 agccaagcca aaatccgatt aatttggtag gtcggcattc caatctggtg aagaaaagct
     5941 cgtccgtcct tgctaataaa aatagctaag aaaccagcaa ctcctgttac agcagtcaca
     6001 aatactggga taataatttt ccaagaaacc cctgataata aaacgattcc tgagaaaatg
     6061 gctacaaaaa ccaaagccgt ccccaagtca ctttgaagtg ctaaaagaac taggactgga
     6121 atggtaaaga gaatcatcca gaaaattaac aaaaagtcca gcggaaccgt gcgtctccat
     6181 tccttatgtt tctttgtaaa ttggacaatg acacgagcca acatgaggat ataggatatc
     6241 ttcataaatt ctgacggttg aaatagggta attccattta ttgataccca gtttttggca
     6301 cccgttgatg caactaagct tggattataa aatacaatcg gcaagatcat aagtcccaag
     6361 cctaaaatat atagaaaggg ggtcaccttc caaagaaatt ctgtattaaa gagcatgacc
     6421 acaaaaccaa tcacaagccc caaggcaatc caggcgacct gctgccctaa aatgggcaga
     6481 atattattgg gataatcatg actaacggct atatagatag ccaccacacc gatgaccagt
     6541 agaaaaaata ctggcaagag caaactgtaa tcgactcttg agtcgagaga acgtttcata
     6601 tatactaacc ttatactttc atacaatact atttatcaaa gttcattaaa aaatctatca
     6661 atagcctcgt caacttcgga tcgagagatg gttttaacag tcgcttcttc tgctagagat
     6721 gctactattt gtttgccgta tcgttttccg acgattctcc tatccaaaat aagagttaag
     6781 gaacgttggt attcacgtct catacttctt cccaaagcct gttttaaacg aataatggcc
     6841 attggcaatt gataatcata aaaggcattt ttcccttctt gattcagttc ttgattaatc
     6901 tttttcgtca agggttcttg aggattttgg aaaggaagcc tcggtacaac ttgaatcaca
     6961 aaaggatggc ttgaaaaatc aactccctcc cagaaacttg ctgcaccaag caagatttgt
     7021 tgttcacctt tttcaaagcg tttctttagc tgatgaacat ccccattttt atactgggcc
     7081 aagtggctaa ctgtaagtaa atccgatact gctagaagca tgtctttagc ggtaaagaga
     7141 accaaaatcg gttgctggaa agcttgaatt tccactagta aagcagctac ctctctggca
     7201 tagacttcta aggaggtttc tgttaccaga gggaaatctt tgaccatgac cacttcttgt
     7261 tcctgttttc cccgagattc aatcttgaca aatttggctt caggatagcc taaaaggtct
     7321 gccaaagaaa ccctctgact aatctcaaga gtagctgata ctcccaagac ttggcaactc
     7381 tcaggaagta aagaggacag aagagtacgg cctgattttg tagaagaaat ctgaattttc
     7441 ttttgactcg tttcagttac ttcaagccag taatcacctt cagctgtaaa atagcgaacc
     7501 agctcatcaa agccttccac ttctaattct gaaaaatact gatggagatt gtccagagaa
     7561 tctaagatat ttttcctaga tttgccagac tgaaattgtt ctatcaagta gagacactca
     7621 aagcgaatac tttctagtat ccgttgttga accctgtttt cttctcctac taaagcctta
     7681 tcaattaaat cgataataga ttgtatatcg taggtctctt gaagcagatt ttctagagct
     7741 aacaaaatct tttggacttc atcaataatc agtaaacggt cactgacaaa ttcaggatta
     7801 tcttcaagtc tggtcacgag ataggcatga ttagtcacta aaagcttgca agtctctgcc
     7861 ctttcttgac tacgtttcca aaaatcttcc gtcacaaata agctctggga tgataaattc
     7921 ccatcatgac gaaggtctgc tagaaaatgt tggtaacggt agagttgccc gatttcatcc
     7981 aaatctcctg tctctgtctc agtaagccag accaagactt gcattttaaa gcgtctaaat
     8041 aagcgatttt catcattttc ctgcaaggaa tgataaaagg catccaactt cagataattt
     8101 tgtggtccct ttaagctatg aatatctgta tggaacactt ccttgagacg tttaccttct
     8161 tcttccatga tttgattttg aagaatcttt gtcggaacac taagaacaat ttgtcgctct
     8221 ttggattgag ataaagcggg taagagatag ccataggttt tcccaatccc tgtcggcgct
     8281 tgaatcagag agacaggttc atctttcaat agcaagccaa cctctttagc aaaactttct
     8341 tgttcctccc tcacttcaag gttcaacaga gaaatatttt tagaaaagtc ttgagatagt
     8401 tttcgtggct ccagagaagc tgccgttttc ttaaaataca gaccttgaac ttggaccaag
     8461 tctggagaac tcaggataga ttggttgcga taagtttcct caataaccag gtaggactca
     8521 tataggagag cgtcagccat ttccagcaag cgttccaaaa gacctttagg aagctgggtc
     8581 atcttttccc gtaaaaaaag aagtaattct gctgtagctt gggcatctga aagggctgtg
     8641 tgtgcgtgtt taagaggaat tcctaattct cgacacaaaa tcggcaagct atatttttcc
     8701 agttcaggga aaaagacctg ggccaattcg accgtatcaa cacgagggtt tcttagctca
     8761 tagccttcaa aaaataaatt ttccgccaag agattagcat caaactgaac attatgggct
     8821 acaaaaatcc catcctccac caagtcaaat atttttctgg caacttgcga aaaatcaggt
     8881 gcttgcgcca gacgttggtc tgtcaatcct gtcagttctt tgatatgagc atccaagggt
     8941 tcatgtggat tgacatccgt cgtatagtga tcgacgattt ctccgtcctc aatcacgaca
     9001 attcccactt ggataatttt agccttacta cctgtgctag ttgcctctaa atccaccaca
     9061 acatagcgat taccaatcgt tttcattata aaaaattata ccaaaaaaag ggccatttgg
     9121 cacctgcaaa cataggataa ttttcaaact caagaacact acttttctta aaaatcccat
     9181 cgaatagagc attctagcct gctgattttt taaaatagtg aagaaatacg aaaaattgag
     9241 agaagaaatg ttttcatctt ccttctctca acgaatcatg tattttattt aaccatgtca
     9301 ggatcgtagt cataaaatcc tgtatcaagg aaacggtttt tagggtgaag tttacgaact
     9361 tcttcatcca gcaagaaggc ttggtcatgg ctaaagttgc cctcttgaat caagctacgc
     9421 gcttggataa agagttttgc aatctcaaca aagttctgac caggagtgta gagcccttct
     9481 agtttccagc gagtaaagcc atgctccacc aattctgtta atttgatcat caaatcaagg
     9541 tcattgttgg caaagatatg ggtcccatga ttatcttcaa aaatggaata gtggctctct
     9601 ggatcacttg gctcagccaa gaagaggtca cgtttatgcg tcttttcatc atcgatatgt
     9661 gtaaagttat agtagttttg caagagtgga cgtttagaat gatggatgac gctagcaccg
     9721 taaaccaaaa cttcagcagg aatttccaaa atctctggca ttttgaaaag ttcagctgat
     9781 ggaatttcac gcgccaaaac agcctcagat gcgccagcct tttgtcccca gaagttaatc
     9841 tgacgactgc tagttaccat ggttgaagca tcgtagatgg tcttaaatga ataaccatcg
     9901 cggttaacta cgtaaaagac gcctgcatcc ccaatcgtaa tatagtctgt cttgatttct
     9961 tccaagaagt ctaagaaagg cttgatacgg tccatcatat cttggtgcat gagagcattg
    10021 accgcaacga tcaattcctt accagcatca tgaaccaact tagcgatttc acgtaattgg
    10081 tcataactaa aggtcgttgg cagacgaagg ccaaaatctt tctcaccgac atagatacgg
    10141 tctacgccag cttcgagtag ttgttcaact tgttcaatac tttcagcagt tgctgtaatg
    10201 acaatctttt ccataagaaa aattatacca catttctcca aaatcagcca tgctttgaaa
    10261 atgaaaaggg actttattct ttttgtaacc tttctgtaat aattctctac cgaaaaaatg
    10321 ataaaatagg tatgtactgt taaggagaga atcatgcccg taagaaaatt acaatcctat
    10381 gaggtagact atcaagaaga attaaaccag cagcttcctc attaccaagc ttatacacct
    10441 gaagcgcaat ctgatgccaa tctcaaggaa atcctatttt ttattaatat cgctgttttt
    10501 tgtatctgta ttgctatctt tagttttatc tttttagcat taaaattatc aactgctctt
    10561 gcctttgccg cagcaatcgg attcagctta cttgttttaa aagttcaacg gtctattatc
    10621 aaacgaaaac gtagaagata aatccaaaat atcgccccaa ttggagcgat atttttattt
    10681 tttcttttta gatggtgtct gaatggcaat cttcacttca aagattgttc ccttgggttt
    10741 attatctttg acagtaacag ttccttttag agcatctaca atttgcttgg ctagggataa
    10801 tcctaaacca aaaccacctt tttgccgggt tctagccttg tctactcgat aaaaacggtc
    10861 aaaaattttc tttttatctt ctgtcgaaat accgattcca ttatcagaaa caagtaaata
    10921 aagattgcga tcggtcgccg agataagaaa atcaatttca ccatcctcct cagtatactt
    10981 gacggcatta tcgaaaagaa tggtcatcag ttgtttcaga agaagctgat ctgtgacaat
    11041 tgttcgatgg atacgatttt caaaacggaa gacacgatta ttttccgaag caatcatctc
    11101 gtagtttgtg aaagttgtat taaaaaagct agttggaact tctgcaagct ccggcttaat
    11161 cccatcatct ctccgagcta agttcagcaa gcttgtcgtt aaaaaacgca tatttcggac
    11221 ttcttccaaa ctcgatgcaa tgctttcgct cacatccata atggtagctt ctggcttacg
    11281 aaaaagggtc tctaagcgat tttgcaaaac tgcgagtgga gttcgtaact catgactggc
    11341 attttccaca aaagactgtt gcttctgcat actctcaagc aggggcctaa cactgaccct
    11401 agctagatag agactggcaa gtaaagacaa aatccagaaa ctagccatca cgaccacaat
    11461 caattgctca tgcttttgac tagcctgctc caactgactg gtattaatca agacagcagc
    11521 atactttata ttggttgaaa ccgaactaat attggtctcc atcaaaatca cacgatagat
    11581 ttcttcctgc ccatagctat taaaaacctg aatctggtag atatgtccta gttctttctt
    11641 ttctaactta atcttatcca agcccaaaaa tcgatttcca gaaagaagtt gagtaaagtc
    11701 tttatcaaag agaatgactt ccgtattgga actgacatta ggttttattt ctactttact
    11761 agcgtccgcc ctagcatttt ctaaatcttt aatctcttct gttgccctat ttatagccag
    11821 ctgaataact gcttgaggat tttcactcag tccatgaagc ttatcgtcca ccgaagtata
    11881 aagactcgaa tgcatgactt gtaaaataat cagagtcatt gtagaaaaaa tcagggtgaa
    11941 gacaccgaag ttgcggataa aataactaaa gtcatccgca taccatgttt ttttaagttt
    12001 actgaacatc ttttaaaaga tacccaacac tacgcaaggt ttgcaaattc tctgcaaaag
    12061 tggttccctt taatttctta cggacttttg aaacatagac ttcgacaacc gaaatcgttg
    12121 tatcactatc aaatccccat agacggtcaa aaatctgcgt cttaggcaaa atcacatttt
    12181 gattttgaag gaaataaact agtaaatcga actctttccc cagcaattcg acaggagtat
    12241 cttcaacttt aacggtattg gttgataaat taaccacgat attcccataa gtcaaggtgt
    12301 tttcattaaa cttccctgaa cgtttgagaa gggcctgaat ccgcatttta agttcttcta
    12361 ggtagaaagg tttggtcaga taatcatccg ctcccagttc aaatccatgt cccttgtcat
    12421 ccaaactttc cttggcagtc ataatcagaa ctggtgtcgt aattcccttt tcacgcaatt
    12481 cttttaagac ttggaaacca tttttttctg gcaacatcaa atccagcaaa atcaagtcat
    12541 agacaccact ctcagcttcg tagagacctt cttctccatc aaatacctgc ataacatccg
    12601 caaaatcgtc taaaaagtca aatactgaat ttgacagacc taggtcatcc tcaaccaata
    12661 agatttttat catgagaaac tcctccttat taaaactatt ataccaaatt tgccttaaaa
    12721 aaaactcaac tctctgcatt ttacatgaga tagctgagtt ttctttttat tttaggctta
    12781 tttatgcatt tccgtattga agaacaactg cttcgactgc agctttttca cggctaatca
    12841 agtcaacacg tgctgcaatt tccttgattc ccataccgat gttacggcta agagcaaggt
    12901 cagaaagttg cggctcaaag aactccttgt attccgccaa gcgttgctga gtcttaaata
    12961 catgagcagg aaggataaca aagctatcaa agctcatatc tcctccaagg gctgccttaa
    13021 tccaagccca gttttcacgc gcccaagacc aagctgtttt ctgagttgct tgatgagcta
    13081 ggaattggta ataccaagca gacaagtcct gtggtttgac cacaaatttg tccttccaag
    13141 aagtaatcaa gttttggata ttatccgcat ctgtactgta tgcaagagct gctgccaact
    13201 ggcgtttaaa gacagcatct gttgcgtgag tataagtatc aagataaagt gctaacaagt
    13261 ctttagtctc atgatgtttc atctcattaa tcagaacttg tgaacgaata gctgctggga
    13321 gttctgcaag attctccttg tgtgttgcga agatttggct agcgacttga cgagcttctg
    13381 catcatttga gcgaatcatc atcgaaacag ccagctgacg aaccaattca tcctcatctg
    13441 attctccgtc tttagcttca aaaccaagac ggtcatagtt atgacgagcc aatttagcaa
    13501 ccagtccttt gaaggctgtt tcagcatccg ttccttcatc aataaagcgc tcaagggctg
    13561 aaatcacttg agaaacagct gaaaccacca gataagactc ttccttagca agtttatcaa
    13621 gaactggaag caagtctgca taagaaatgt gccctgcctc agccaacaaa cgacgttctt
    13681 gaacaatttg cagtttgctt gtgttatcaa gtgtctctag ctcagcaaga acagtttcta
    13741 acaagtctcc ttgatagtcg gtaatatagt gggcagtatt ttcagtgttg agacgaagag
    13801 ctccttcatt ttcagcaaga agagctgcgt agccagggat ttcgatactt tcagtttcga
    13861 gtgtatcagg caagcctttc cagttgctat tgaggggcac cacccagaga cggttcttgt
    13921 cttcgttctc accgatgaag aattgttttt gtgaaatctt caagacatca ttttcaactt
    13981 tgacagtaag aactgggtaa ccaggctgtt ccaaccaaga atccatgaag gctgcgacat
    14041 cacgtcctga cgcttgacca agggcatccc aaaggtcact accaatggtg ttgctgtatt
    14101 ggtgtttttc aaagtaggca tgcaaacctt tagcaaaatc agcatctcct agccaacggc
    14161 gaagcatgtg catgagacgg cttcctttgg catagacgat agcgccgtca aagagtgtat
    14221 tgatttcatc tggatgttta acttcgacgt ggacagactg aacgccatca gtagcgtcac
    14281 gttcaagagc aagaggtact ccacctgttt ggaaatcttc aaagatattc cagcttggtt
    14341 cgatggtatc cacacagacg tattccatca tattagcgaa actttcattg agccaaaggt
    14401 catcccacca tttcatagtc acgaggttcc caaaccattg gtgagccaat tcatgggcca
    14461 caacaagggc aacttgttga cggctagcaa atgtagagtt ctcatcgaca accaagtaaa
    14521 cttcacggta ggtcacaaga ccccagtttt ccatagcacc agctgagaag tcaggaaggg
    14581 cgatgtggag agattgagga attggatact taactccata gtaatcttcg taaaactcga
    14641 tagagcgaac agcgatatcc agtgagaaat caagatttga aagtggatgt gctttggttg
    14701 agtagacacc taccagggta ccatttttag ttttagcggt caccccttgc aaatcaccag
    14761 caacaaaggc caacaagtaa gaagacatgc gaggtgttgt ctcaaacttc cagatacctg
    14821 tttccttacg gttttcaaca tcgatttctg gcatgtttga caaggccaat tcaccttctg
    14881 cttggtcaaa gcgaagagag aggtcaaaag ttgctttggc ttcaggctca tccacacatg
    14941 ggaaagcttc gcgcgcaaaa tggctctcga actgagtaga caagacctcc ttcttgactc
    15001 catcaactgt ataataagaa gggtaaatcc ctgtcatgtt gtctgtaatt ttaccagaaa
    15061 aggcaagaac caattcaact tgaccagcct cagccaattc gatatgaagg gcttcattgt
    15121 catggtcaac tgtaaatgga cgagcttgac ctgcaacttc tacagaggtg atttccaagt
    15181 ctttttggtg gagggagatg cggtcactct gtgcttgacc agtgatggtc actttcccag
    15241 aaaaagtctt ggtctcacga ctcaaatcta aaaataaatc ataatgttca ggaacaaatt
    15301 gcttaataaa atgttcaact gcttgcatag ttttctccta ttctaagttt aagagctgct
    15361 gctcttcttc aaacaaactt attatatcat attttgcttg aaaaaaaagg attggacagc
    15421 gatttccaaa actttcttcc ttctaatagt ctacagtggg tagaccttgc gaaattcttc
    15481 taaaacaacc ttgctttcag atgtaaaagt cagccctact tcacccaaac agtctgtgaa
    15541 aaagtcctca tgaacctggc gccaataggc aagtgatttg tctccctcac cttccttaaa
    15601 ggcatgttgc gcagaaactt gattgaagag ttcaacagaa acctttgtaa tttcgacaat
    15661 gcagacagcc tgattttgac tatctaaaat gatatcgaag gtccctactt ggggaagtgg
    15721 ttcgtcttct agtacataga ggtcataggc tgatgctgtt gctgtctttt ctcctttaaa
    15781 caccaaatcc gctaaaagat ctggttcaac tccaaaagcc caagcatcga tttcatctcc
    15841 gatcaaagga ttgatttgct tgtatttatt ccacatttct tgcggtatca tgggtactcc
    15901 tttgtaattt tttactttct tcttttatgt gtttaagatg atctggatgg tcaatctcta
    15961 aatcaaaaat ctctggaata gaactgtagt ggataatgca cttgataccc aactgattca
    16021 ttttttgtat gaaagaagta ttcagatagc ctgctacagc aaaatcaatc ttgttctttc
    16081 ttgctttatc ctgcatatct cttagcatat ctaacattat tggactttcc atatcatgcc
    16141 attgactgtt tctcatagtc gcaaaaacaa aggaagtcaa atcattcatt ccaactacaa
    16201 tctttgaaat gcccgtttcc agtatactag ataagtcaaa atacgctgac ggtaattcaa
    16261 tcatcgttcc gactttccca gtaaaaccct gctgacgcaa tactgtaata gcttgtttta
    16321 attggtcgtc atcattgaca aaaggaaaga taacagatag attggggttg gtttgataaa
    16381 cttctgtaac gacatgtgct tcagcctgaa attcatccaa acacgccagt aaacgcctgg
    16441 ttcctctata gccaaacaag ggatgccctt cgtcaaaaaa ctctctagtc cccactaaac
    16501 aattggcttc tgtattcgtt aattcagaaa aacgatacca aacttcttca tctgagtaca
    16561 gggagcagat agtctctaga taatctttta caaattgctg acaacttgct aatatgatgt
    16621 tttgattaag ctctctcaac aaatattccc cacgaatcat gccgacgtgg tgaaatagtt
    16681 gaggataaat tttttcaaga attttttcgc cactaagggc aagttgattt ctcatagcca
    16741 ttcacctcgt cttaaaagat acaatatcat atataattat atcaaaattt cctctagttc
    16801 aaaagcttca taaggttcag ataactcata tcctaaaaat tctgctactt ttttggaagc
    16861 ctcattatga gcatcccaaa gtggaaacat atctctattt aaactctcta gaatcattgc
    16921 agcaccaagc tttttagcaa atccatttcc ttgttcgttt ggtctagttg caacttccac
    16981 ttcaactgct ttacggtaaa ctaaccctga ggaaatccca gcaatcagtt cattattttt
    17041 aagaatcaca aagccaaatc caccttttaa aacaaaatcc tgataggact caaaatcccc
    17101 ctgtaaatcc tgtgaccatt cttccgtaga aaagcagtta taaatatgat tatcaatagg
    17161 cacaatattg taaccttcct ctaaatgagt aactagatta tttaaaaatt caacttgaaa
    17221 atttgc
//
LOCUS       DAVKZB010000032        17020 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKZB010000032 DAVKZB010000000
VERSION     DAVKZB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.032"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(44..403)
                     /locus_tag="VXA80_000964"
     CDS             complement(44..403)
                     /locus_tag="VXA80_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV0353481.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            495..1451
                     /locus_tag="VXA80_000965"
                     /pseudo
     CDS             495..1451
                     /locus_tag="VXA80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            1435..1992
                     /locus_tag="VXA80_000966"
     CDS             1435..1992
                     /locus_tag="VXA80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0353482.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            1985..2239
                     /locus_tag="VXA80_000967"
     CDS             1985..2239
                     /locus_tag="VXA80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353483.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            2251..4305
                     /locus_tag="VXA80_000968"
     CDS             2251..4305
                     /locus_tag="VXA80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV0353484.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            4484..5344
                     /locus_tag="VXA80_000969"
     CDS             4484..5344
                     /locus_tag="VXA80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV0353485.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            5555..6265
                     /gene="ccdA2"
                     /locus_tag="VXA80_000970"
     CDS             5555..6265
                     /gene="ccdA2"
                     /locus_tag="VXA80_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0353486.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            6285..6851
                     /locus_tag="VXA80_000971"
     CDS             6285..6851
                     /locus_tag="VXA80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV0353487.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            6862..7974
                     /gene="msrB"
                     /locus_tag="VXA80_000972"
     CDS             6862..7974
                     /gene="msrB"
                     /locus_tag="VXA80_000972"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0353488.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            8242..8979
                     /locus_tag="VXA80_000973"
     CDS             8242..8979
                     /locus_tag="VXA80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0353489.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            8976..10667
                     /locus_tag="VXA80_000974"
     CDS             8976..10667
                     /locus_tag="VXA80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0353490.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLTYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFEANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            10783..11346
                     /locus_tag="VXA80_000975"
     CDS             10783..11346
                     /locus_tag="VXA80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV0353491.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            11368..17013
                     /gene="zmpB"
                     /locus_tag="VXA80_000976"
     CDS             11368..17013
                     /gene="zmpB"
                     /locus_tag="VXA80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpB"
                     /protein_id="HEV0353492.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEQAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TKTAKGTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTTEVVPDDTKYVDEEV
                     VERQGSKGVQVTKTTYETVEVVETDKVLSTTTEVKTPVVPKVVKKGTKPVEGTAVETR
                     EEVIPFATKEQEDDTLKRGTRQVAQEGVNGKKQITETYKTIRGEKTNEAPTVEETVLQ
                     APQDEIIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVEIKSIKVALKDNT
                     GTVIKEVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETEMLEDKEVQLDLKKV
                     EIKSIKETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKTTRLAVSSVEEVV
                     VDGKTLYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYNFNELVKDMQANP
                     TGEFKLGADLNAVNVKPAGKAYVMAKFRGTLSSVENHQYTIHNLERPLFNEAEGATLK
                     NFNLGNVNINMPWADKVAPIGNMFKKSTLENIKVVGSVTGNNDVTGAVNKLDEANMRN
                     VAFIGKINSLGDKGWWSGGLVSESWRSNTDSVYFDGDIVGNNSKFGGLVAKVNHGSNQ
                     WDVKQKGRLTNSVVKGTMTLKNHGQSGGLVHENYDWGWVENNISMMKVNNGEIMYGSG
                     SIDGDPYFGFDYFKNNYYVKDVATGESTYKRSKQIQSISQAEADAKIANMGITANTFA
                     IQDPVVNKLNRIIDRDSEYKAIQDYQETRNLAYRNLEKLQPFYNKEWIVNQGNKLTDE
                     SNLVKKTVLSVTGMKSGQFVTDLSSVDKIMIHYADGTKEEFGVSAISDSRVKQVKEYN
                     VDDLGVVYTPNMVDKNRDSLITKVKEKLSSVALDSAEVKSITNNPASLYLEESFAEVR
                     ETLDKLVKSLLENEDHQLNSDEVAEKALLKKVEDNKAKIILALTYLNRYYGIDYDGLN
                     FKHLMMFKPDFYGKTPSILDFLIRIGSAEKNLKGDRSLEAYREVIGGTIGKGELNGLL
                     GYNMRLFTKYTDLNDWFIHAAKNVYVSEPETTTEDFKDKRHRIYDGLNNDVHGRMILP
                     LLNLKKAHIFVISTYNTIAFSSFEKYGKNTEEERNAYKAEIDRVAKAQQRYLDFWSRL
                     ALPKVRNQLLKSQNSVPTPVWDNQVYVGLGGANRMGYGDGGRVVTPVRELFGPTDRWH
                     QINWNMGAMAKIYERPWKDDQVYFMVTNMMEPFGISAFTHETTHVNDRMAYYGGDWHR
                     EGTDLEAFAQGMLQTPDKSTTNGEYGALGINMAYERKNDGEQLYNYDPEKLDSREKID
                     SYMKNYNESMMMLDYLEASAVIRQNLSDNSKWFKKMDKEWRTNADRNRLIGEPHQWDK
                     LRDLTEEEKKLPIDSIDKLVENNFVTLHGMPKNGRYRTEGFDSSYQPVNMMAGVFGGN
                     TSKSTVGSISFKHNAFRMWGYYGYENGFIPYVSNKLKGAANKENKGLLGDDFIIKKVS
                     KNQFQNLEEWKKHWYHEVYDKAQKGFVEIEVDGVKISTYAQLQSLFEEAVSKDLAGMD
                     DKNIKNHYQYTENLKWKIYKQLLKNTDGFSSDLFTAPQA"
BASE COUNT         5260 a         3008 c         3841 g         4911 t
ORIGIN      
        1 tgtagagaaa gcaaaaatcc ctagaaaatg cttagggatt cgactatttc attaagaaca
       61 attcaacaat catttctata tacatgataa gggttaataa gaatcctgca cgccagaaga
      121 atttgatgaa tttaggatag taaaagctgc gtttttttaa aaggaagaaa aagacgagaa
      181 taatggctag gaacgagagg gctagcccca gtctagggag gaaattatgg gtaaaggttt
      241 tagctgtaat caagtaatac tcaaatacca agactggaaa agccaaatcc gcaaagtttc
      301 gtcctagttt ttttaatcta aaaagtttgg ttaggataat gcagacgact aaggtcagta
      361 tcaataataa aatagatgct aatttcatta aaatcatacc catattgtat cataaaaaaa
      421 ggctaatgga aacgagaaac agaggaattt ataggaaagt caggcttttg agattgtggg
      481 tgatttttgt tataatgaaa gttataaaat cttatgatac cttgaatgat tattatcgaa
      541 aactctttgg agaaaagact tttaaagtcc ctattgatgc gggatttgac tgtcccaatc
      601 gtgatggaac tgtggctcat ggaggctgta ctttttgtac ggtttccggt tctggagatg
      661 ccattgtggc accagatgcg cctatccgtg agcaatttta taaggaaatt gactttattc
      721 atcgtaagtg gccagatgtt cagaagtatc tggtttattt tcaaaatttt accaacaccc
      781 atgaaaaggt ggaagttatt cgagagcgtt atgaacaggc tatcaatgag ccaggtgtgg
      841 taggaatcaa tattggaacg cgcccagact gtttaccaga cgaaaccatc gaatatttgg
      901 ctgagttatc ggagtgcatg catgtgacgg ttgaattggg cttgcagact acttatgaag
      961 caacctctga cctgattaac cgtgcgcatt cttatgaatt gtaggtggaa acggtcaagc
     1021 gtttgagaaa gtatccaaaa attgagattg tttcccattt gatcaatggt ttgcctggtg
     1081 aaacccatga gatgatggtt gaaaatgtcc gccgttgtgt cacggataac gatattcaag
     1141 ggattaaact gcatttgctc catcttatga caaatactcg tatgcaacga gattaccatg
     1201 agggacgttt acaattgatg agtcaggacg aatatgtcag ggtcatctgt gaccaactgg
     1261 aaattattcc caaacatatc gtcatccatc gaattacagg agatgcgcct agggatatgc
     1321 tgattggtcc tatgtggagc ctcaataaat gggaagtcct caacagcatt gagatggaga
     1381 tgagacgtcg tggaagtgtt caaggatgca aggctgtaaa acaggagttt gaaaatgaaa
     1441 agaccacttg agatggcaca tgattttttg gctgaggtcg tgacaaaaga ggatgtcgta
     1501 gtggatgcga ctatgggaaa tggtcatgac acgctttttt tagccaagct agccaagcaa
     1561 gtctatgcct ttgatattca gaagcaagcc ttggaaaaga cccaagagcg tttgcatcag
     1621 gctggcttga caaatgccca gttaatcttg caaggccatg agacactgga ccagtttgtg
     1681 acaaaagcta aggcagggat ttttaatttg ggctatttgc cgtcagctga taagtctgtc
     1741 atcacccgac cgcagacaac gattgaggca ttagaaaagc tatgtggctt acttgtcaaa
     1801 ggtggacgaa ttgctattat gatttactat ggtcatgaag gaggcgacct cgagagagat
     1861 gctgtcttgg attttgtgat ccagttgaac caacaagagt acacagctgc catttaccga
     1921 actttaaacc aagtcaacaa cccgccgttt ttagtgatga ttgaaaaatt agagagatac
     1981 agacatggat aaacaatacc tacacgaaaa gctggatgcc atgcgccaga attttgttga
     2041 atcaacctac cacgaacgag cggtgggcgt gctagaccaa gcgcatatga gcaaaaaaat
     2101 gcttaaaatc aagaaaaaat tagttgctct tgaaatggaa cggtgccaga gaaaaattga
     2161 gcacaaagac tgttccaaga ttgaccaaaa aatcaaagag cagaaggaga tatttgaatc
     2221 ctgttgtaaa aaagattaag gaggacgtgc gtggaattac tgatttacct catcctattt
     2281 ttactggtct tgattgtctc aagtacaacc aataagctcc tacccttttt gcctctccct
     2341 ttggtgcaga ttcttttggg aattgtgatt ggtctctttt tacccaatac tgactttcac
     2401 cttaatacgg agttgttttt ggcactggtt atcggaccct tgcttttccg agaggctgaa
     2461 gaagcagatg ttacggctat tttaaaacac tggcgaatca ttgtttatct catatttcca
     2521 gtgattttta tctcgaccct gagtttgggt ggcttggccc atcttctttg gttcagcctt
     2581 cccttggcag cttgcttggc tgttggggca gcccttggtc ctacggactt ggtggccttt
     2641 gcctctcttt cggagcgttt tagctttcct aagcgcgtgt ccaatattct taagggcgaa
     2701 ggactcttga atgatgcttc tggtttggtg gcttttcagg tagctttgac agcttggaca
     2761 actggagctt tttctctggg gcaagctagc agttcgctca tcttttcaat cctaggcggt
     2821 tttttaattg gatttttaac agccatgacc aaccgcttcc tccatacctt cttgctaagt
     2881 gtgcgcgcaa cggatattgc cagtgaactt ttattagaat tgagtttgcc tctagtgacc
     2941 ttctttctgg cagaagaagt tcatgtttca ggtattattg ccgtcgtagt tgctggaatt
     3001 ttaaaggcaa gtcgcttcaa gaaaatcacg ctcctcgaag cccaagtgga tacggtgacc
     3061 gagacggtct ggcatacagt gacctttatg ctcaacggtt ctgtctttgt gattttaggg
     3121 atggagttgg aaatgatagc agaacctatc ttgaccaatc caatctataa tcctctactt
     3181 ttattgctat ctcttatcgc ccttaccttt gtcctttttg tcattcgttt tattatgatc
     3241 tatggctatt atgcctatag aacccgacgc ctcaagaaaa agctaaataa gtatatgaag
     3301 gacatgtttc tcttgacctt ttcaggtgtt aagggaacgg tgtcgattgc tacgattctc
     3361 ttgataccaa gtaatctaga acaggagtat cctcttttgc ttttccttgt tgcaggtgtg
     3421 acgcttgtta gctttttaac aggtctcttg gtcttgcctc atctttctga tgaagaggaa
     3481 gaaagcaagg attatcttat gcatatcgcc attttgaatg aagtaacgct agagttggaa
     3541 aaagagttgg aagacaccag aaataaactt cccctctatg cggctattga caattatcat
     3601 ggacgtattg aaaatctcat tttaagccaa gaaaaccagg atgatcaaga agactgggct
     3661 gctttgaaac tcttgattct tagtattgaa agtgatggtt tggagcaggc ctatgaagag
     3721 gggaacatta gcaatcgtgc ttaccgagtt taccaacgtt atctgaaaaa tatagaacaa
     3781 ggaatcaatc gtaaacttgc ctcaagactg acctattatt tccttgtttc tttgcggatt
     3841 ttacgttttc tccttcatga agtttttact ctcggaaaga ccttccgtag ctggaagaac
     3901 aaggagcaaa gccgtctccg tgctcttgat tatgaccaaa ttgcagagct ctatcttgcc
     3961 aatacggaga tgattattga aagtttggaa aacctgaagg gagtctacag acgctctttg
     4021 attagtttta tgcaggagtc tcgtcttcga gaaacaacta ttatcagcag tggtgccttt
     4081 gtcgaacggg ttatcaatcg tgtcaaaccc aacaatatca atgaaatgct gagaggctat
     4141 tatctggagc gcaagttgat tttcgaatac gaagaaaaac gattaattac aactaagtat
     4201 gccaagaaat tacgacaaaa tgtaaataac ttagagaact attccttgaa ggaagctgcc
     4261 aataccctgc cgtatgatat ggtggaattg gtaagaagaa attagttaat actcttcgaa
     4321 aatctcttca aaccacgtca gcgtcgcctt accgtatgta tgtgactgac ttcgtcagtc
     4381 ttatctacaa cctcaaaaca gtgttttgag cagcttgcgg ctagctttct agtttgctct
     4441 ttgattttca ttgagtataa gattataaat gaaggagtat gacatgaaaa agtggtggaa
     4501 agagctgata gacaagcctt tattaaaagc ttttttgcat tattatcaag catcagatag
     4561 tgagttgacc agtgtcgcag tagcctacta ttggttgatt tcgattttcc ccctgctttt
     4621 ggtggtggtc aatatcctcc cctattttca gattcctgtg ggagaatttc tgggctttat
     4681 gaaagacgtc ctgcccccaa gcctctatga aggtgtggaa aaaatagcca gagaagtctt
     4741 gacgcaacct tcaacaggtt tattgagttt ttctgtttta tcggcgttat ggagtttttc
     4801 aaaatctatg aatttcttgc aaaaagcttt taacaaggct tatggcgtcg aaaagagtcg
     4861 tggacttatt tcccatcaga tgctaagtct cctagtcagt tttggcttgc agctcctctt
     4921 tgcgtttgcc ttgtttttga ttttatttgg tcagatgatt ttggctttac ttgctcatta
     4981 ttggacaaaa gatggcatta tctatcaggc tttacaaggg ttggcaggtc ctctgattta
     5041 cgccctcctc tttgctatct tggtcatgct ttattatttt cttcccaatc tatctaacag
     5101 gaaaattagc tataccctac caggcagtgc ctttgttctc ttagtgattt taggtttact
     5161 aactctgttc tctagttatc tcaattacta tgtccatcat ttggtggatg tccgaatttt
     5221 aggttccgtt cttcttgttg ttatgatgtt ttggtttatc ttgattgcta aaattgttat
     5281 cctaggcgcg gttatcaatg ccagtatgca gagtttgaaa gatccggtct ttaaaaaaga
     5341 ctaactattt atccattata aaagcgcata gtatcaagac tttatagaac cttgatacta
     5401 tgcgcttttg tgctttgaca gttttttgta tagtttttgt ccaagatttc taaatcatct
     5461 gattaaattc ctattttccc ctatcattgt ccctgttttt tcgaaaatgg aaggagtata
     5521 ataaatctat caataaaatg ataggaggaa ctttatgggt catattttct tttttctaag
     5581 tgtctttttg gcagggattc tatccttctt ttctccttgt atcttacctt tgttaccggt
     5641 ctatacagga gtgttactag atgataagga tggtgctcag gcttctagcg gcaaattttc
     5701 aatctcagtt actagtttat tacgaactct ggcctttata gcaggaattt cctttatatt
     5761 tattttgttg ggctatggag ctggtttttt aggcgatttg ctttatgctt cttggttcca
     5821 atatcttact ggggcaatta ttatccttct tggtttgcac caaatggaga ttctacactt
     5881 taaggggctt tataaggaaa agaggctaca actgcaagga caggggcaaa atggtaaggg
     5941 ctatagtcag gcatttttat tgggcttgac ctttagtttt gcttggacgc cttgcgtggg
     6001 gccggttctg gggtctgttt tggccttggc ggcttcaggt ggttcaggag cttggcaggg
     6061 agctggtctc atgttggtgt atacgctggg cttggcgcta ccattcttgc ttctagctct
     6121 gacctctagt tatgttttga aacatttccg aaaacttcat ccctatctcg gaatcctcaa
     6181 aaaagtgggt ggttttctca ttattgtgat gggactcttg gttctgtttg gaaatgcttc
     6241 aattttaagt caattatttg aataaaatgg aaaggaatat caatatgaaa aaatggcaaa
     6301 catgtgttct tggagcaggt tcgctccttt gtttgacggc ttgttcaggc aagtccgtga
     6361 ctagtgaaca ccaaacgaaa gatgaaatga agacggagca gacagctagt aaaacaagcg
     6421 cagctaaagg gaaagaggtg gctgattttg aattgatggg agtagatggc aagacctacc
     6481 gtttatctga ttacaagggc aagaaagtct atctcaaatt ctgggcttct tggtgttcca
     6541 tctgtctggc tagtcttcca gatacggatg agattgctaa agaagctggt gatgactatg
     6601 tggtcttgac agtagtgtca ccaggacata agggagagca atctgaagcg gactttaaga
     6661 attggtataa gggattggat tataaaaatc tcccagtcct agttgaccca tcaggcaaac
     6721 ttttggaaac ttatggtgtc cgttcttacc caacccaagc ctttatagac aaagaaggca
     6781 agctggtcaa aacacatcca ggattcatgg aaaaagatgc aattttgcaa actttgaagg
     6841 aattagccta ggaggtgtct tatgaatgat aagttaaaaa tcttcttgtt gctaggagta
     6901 ttttttctag ccataaccgg tttctatgtt ctattgatac gaaatgcagg gcagacagat
     6961 gcctcgcaaa ttgaaaaggc ggcagttagc caaggaggaa aagcagtgaa aaaaacagaa
     7021 attagtaaag acgcagactt gcacgaaatt tatctagctg gaggttgttt ctggggagtg
     7081 gaggaatatt tctcacgcgt tcccggggtg acggatgccg tttcaggcta tgcaaatggt
     7141 agaggagaaa caaccaagta cgaattgatt aaccaaacag gtcatgcaga aaccgtccat
     7201 gtcacctatg atgccaagca aatttctctc aaggaaatcc tgcttcacta tttccgcatt
     7261 atcaatccaa ccagcaaaaa taaacaagga aatgatgtgg ggacccagta ccgtactggt
     7321 gtttattaca cagatgacaa ggatttggaa gtgattaacc aagtctttga tgaggtggct
     7381 aagaaatacg atcaacctct agcagttgaa aaggaaaact tgaagaattt tgtggtggct
     7441 gaggattacc atcaagacta tctcaagaaa aatccaaatg gctactgcca tatcaatgtt
     7501 aatcaggcgg cctatcctgt cattgatgcc agcaaatatc caaaaccaag tgatgaggaa
     7561 ttgaaaaaga ccctgtcacc tgaggagtat gcagttaccc aggaaaatca aacagaacga
     7621 gctttctcaa accgttactg ggataaattt gaatccggta tctatgtgga tatagcaact
     7681 ggggaacctc tcttttcatc aaaagacaaa tttgagtctg gttgtggctg gcctagtttt
     7741 acccaaccca tcagtccaga tgttgtcacc tacaaggaag ataagtccta caatatgacg
     7801 cgtatggaag tgcggagccg agtaggagat tctcaccttg gccatgtctt tacggatggt
     7861 ccacaggaca agggcggctt acgttactgt atcaatagcc tctctatccg ctttattccc
     7921 aaagaccaaa tggaagaaaa aggctacgct tatttactag attatgttga ttaatactct
     7981 tcgaaagtct cttcaaacca cgtcagcgtc gccttaccgt atgtagggtt actgacttcg
     8041 tcagttttat ctacaacctc aaagctatac tttgagctga cttcgtcagt tttatctaca
     8101 acctcaaagc ggtactttga gaaacctgtg gctatcttcc tagtttactt tttgattttt
     8161 attgagtata agaggtcttt cctaagcagt tagaggagaa ttttgctata ctgatactag
     8221 taagtggtaa aggagcagag catgacctac acaatcttaa tcgtagaaga tgaatatctg
     8281 gtaagacaag gtttgactaa actggtcaat gtagcagcct acgatatgga aatcatcggt
     8341 caggctgaaa atggaaggca ggcttgggaa ttgatccaaa agcaggtgcc agatatcatt
     8401 ttaaccgata tcaacatgcc tcatctaaat ggcatccagt tggccagtct ggtacgagaa
     8461 acctatcctc aggttcattt ggtcttttta acaggttacg atgattttga ttatgccttg
     8521 tctgctgtca aactaggtgt ggacgactac ctgctcaaac ccttttctcg tcaggatatt
     8581 gaggaaatgt tggggaaaat caaacaaaaa ctagacaagg aagagaaaga agagcagtta
     8641 caagatttat tgactaatag gtttgaagga aacatggccc agaaaatcca gtctcatctg
     8701 gctgatagcc aatttagttt aaagtcttta gccagtgact taggttttag tccgacctat
     8761 ctgagttcct tgattaagaa agagttgggc ttgccttttc aggattatct ggtgagagaa
     8821 cgtgttaaac aagccaagct cttgctttta actacagatc tgaagattta tgagatcgca
     8881 gagaaggttg gttttgaaga tatgaactat tttacccaac gttttaagca gattgcaggt
     8941 gtgacacctc gtcagtttaa gaagggagaa gaccgatgaa gcgttcttct cttttagtta
     9001 gaatggttat ttccatcttt ctggtctttc tcattctcct agctctggtt ggaactttct
     9061 actatcaatc aagttcttca gccattgagg ccaccattga gggcaacagc caaacgacca
     9121 tcagccagac tagccacttt attcagtctt atatcaaaaa actagaaacc acttcgaccg
     9181 gtttgaccca gcagacggat gttctgacct atgctgagaa tcccagtcaa gacaaggtcg
     9241 agggaatccg agatttgttt ttgaccatct tgaagtcaga taaggacttg aaaactgttg
     9301 tgctggtgac caaatctggt caggtcattt ctacagatga cagtgtgcag atgaaaactt
     9361 cctctgatat gatggctgag gattggtacc aaaaggccat tcatcaggga gctatgcctg
     9421 ttttgactcc agctcgtaaa tcagatagtc agtgggtcat ttctgtcact caagaacttg
     9481 ttgatgcaaa gggagccaat cttggtgtgc ttcgtttgga tatttcttat gaaactctgg
     9541 aagcctatct caatcaactc cagttggggc agcagggctt tgccttcatt atcaatgaaa
     9601 accatgaatt tgtctaccat cctcaacaca cagtttatag ttcgtctagc aaaatggagg
     9661 ctatgaaacc ctacatcgag acaggtcagg gttatactcc tggtcacaaa tcctacgtca
     9721 gtcaggagaa gattgcagga actgattgga cggtacttgg cgtgtcatca ttggaaaagt
     9781 tagatcaggt tcggagtcag ctcttgtgga ccttgcttgg ggccagtgtc acatctcttc
     9841 ttgtctgtct ctgcttagtg tggttcagtc ttaaacgctg gattgctcct ttgaaggatt
     9901 tgagagaaac catgttggaa attgcttctg gtgctcaaaa tcttcgtgcc aaggaagttg
     9961 gtgcctatga actgagagaa gtaactcgcc aatttaatgc catgttggat cagattgatc
    10021 agttgatggt agctattcgt agccaggaag aaacgacccg tcagtaccaa cttcaagccc
    10081 tttcgagcca gattaatcca catttcctct ataacacttt ggacaccatc atctggatgg
    10141 ctgaatttca tgatagtcag cgagtggtgc aggtgaccaa gtccttggca acctatttcc
    10201 gcttggcgct caatcaaggc aaggacttga tttgtctctc tgacgaaatc aatcatgtcc
    10261 gccagtatct ctttatccag aaacaacgct atggagataa gctggaatac gaaattaatg
    10321 aaaatgttgc ctttgataat ttagtcttac ccaagctggt cctacaaccc cttgtagaaa
    10381 atgctcttta ccatggcatt aaggaaaagg aaggtcaagg ccatattaaa ctttctgtcc
    10441 agaaacagga ttcgggattg gtcatccgta ttgaggatga tggcgttggc ttccaagatg
    10501 ctggtgatag tagtcaaagt caactcaaac gtgggggagt tggtcttcaa aatgtcgatc
    10561 aacggctcaa acttcatttt gaagccaatt accagatgaa gattgattct agaccccaaa
    10621 aagggacgaa agttgaaata tatataaata gaatagaaac tagctaactc ccagtcaatt
    10681 ctgggagttt tatgcgtaat tgggcaaact ttataggttg tctatcttgc taaaaacaag
    10741 aaaaaacggt atgatagaag agtgacaaca gaggtaacag agatgaacga gacacagatt
    10801 caaagagaaa cacgtcaggt agtggaagat gttttagaaa agaccaattt gaagcagggg
    10861 gtcctttttg ttttaggtct atcttctagt gaggtgctag gtggtcagat tgggaaagaa
    10921 tccagtcaag agattggtga gctgatagtg gagactatct tgggtattct aggtagtaga
    10981 ggtatccatt tagcagttca aggttgtgag catgtcaatc gggctctcgt tgttgaacgt
    11041 caggtggcag agcagtttga tctggaaatt gtcagtgtcc atcctactct tcatgcagga
    11101 ggttcggggc agttggcagc ctttaagttt atgcaggatc cagttgaggt tgaatttatc
    11161 aaggctcatg ctggattgga tatcggagac actgcaattg gcatgcatgt caagcatgtt
    11221 caggttccga ttcgccctat tttgagagag attggtcatg cccatgtaac ggcactggct
    11281 agtcgtccaa aattaatcgg aggtgcgcgt gcgcactatc cgcaagatgc tattagaaag
    11341 tcttgagaat gaataaagga gatgaaagtg tttaaaaaag accgtttttc aattcgtaag
    11401 attaagggag ttgtaggctc tgtatttctt ggaagccttt tgatggctcc ttctgtagtg
    11461 gacgcagcta cctatcacta tgtaaataaa gagattattt cacaagaagc taaagattta
    11521 attcagacag gaaagcctga caggaatgaa gttgtatatg gtttggtgta tcaaaaagat
    11581 cagttgcctc aaacagggac agaagcatct gttttgacag cttttggttt gctgactgtt
    11641 gggagcttgc ttttaatcta caagagaaag aaaattgcta gcgtctttct agttggagct
    11701 atgggattgg tagttcttcc tagtgcagag gctgtagacc cagttgcgac cctagcgctg
    11761 gctagtcgag agggtgttgt tgaaatggat ggctatcgct atgttggtta tctatcaggt
    11821 gacatcctca aaacgcttgg cttggacact gttttagaag aaacctcagc aaaacctgga
    11881 gaggtgactg tagtcgaagt tgagactcct caatcaacaa caaatcagga gcaagctagg
    11941 acagaaaacc aagtagtaga gacagaggaa gctccaaaag aagaagcacc tagaacagaa
    12001 gaaagtccaa aggaagaacc aaaatcggag gtaaaaccta ctgacgacac ccttcctaaa
    12061 gtagaagagg ggaaagaaga ttcagcagaa ccagctccag ttgaagaagt aggtggagaa
    12121 gttgagtcaa aaccagagga aaaagtagca gttaagccag aaagtcaacc atcagacaaa
    12181 ccagctgagg aatcaaaagt tgaacaagca ggtgaaccag tcgcgccaag agaagacgaa
    12241 caggcaccag tcgagccaga aaagcaacca gaagctcctg aagaagagaa ggctgtagag
    12301 gaaacaccga aacaagaaga gtcaactcca gataccaagg ctgaagaaac tgtagaacca
    12361 aaagaggaga ctaaaacagc taaggggacg caagaagaag gcaaagaagg tcaagcacca
    12421 gtacaagaag taaacccaga atataaagta acaacaggta cagttgagaa gtctaccgaa
    12481 agtgaattag atttcacaac agaagtagtt ccggatgaca caaaatatgt agatgaagaa
    12541 gttgttgaac gtcaaggatc taaaggagtt caagttacga aaacaactta cgaaacagtg
    12601 gaagttgtag agacagacaa agtcctttct acaactacag aagtgaaaac tcctgttgtt
    12661 cctaaagtcg ttaaaaaagg aacaaaacca gttgaaggaa cagcagttga aacaagagaa
    12721 gaagtcattc cttttgctac aaaagaacaa gaagatgaca ctttaaaacg cggaacaaga
    12781 caagtagctc aagaaggtgt aaacggtaag aaacaaatta ctgaaaccta taaaacaatt
    12841 cgcggagaaa aaacaaacga agctccaaca gtagaagaaa cagttctcca agcacctcaa
    12901 gatgagatta tcaaaaaagg cacaaaaggt ttagaaaaac caaccttaga atgggttaaa
    12961 acagataaag atgtcttgaa gaagagtgcg acagccagtt atacattgaa taaaccagat
    13021 ggggtagaaa tcaaatccat caaagtagct ctaaaagata atactggaac agttattaaa
    13081 gaagtaacgg tagaagaaaa taacttaaac gctactttag acaacttgaa atattatcaa
    13141 ggttatacac tatccacaac catggtctat aatcgaggcg aaggcgaaga aactgaaatg
    13201 ttagaagata aagaagttca gttagacctt aaaaaagttg aaatcaaatc catcaaagaa
    13261 actagcttga tgagtgtgga tgatgctggt gttgaaacag ataagagctt acttactgaa
    13321 aaaccgacag acgttgctcc actttaccta cgagtgacaa ctcatgataa caaaacaaca
    13381 agactggcag taagttcagt tgaagaagtt gttgttgatg gaaaaacatt gtacaaagtg
    13441 gtagctaaag ctcctgactt agttcaacgt agagaagatg atacactaag cgaagagtat
    13501 gtacattact ttgaaaaaca attaccaaaa gtagataatg tgtactacaa cttcaatgaa
    13561 cttgtaaaag atatgcaagc taatccaacg ggtgagttta aacttggtgc agatttgaat
    13621 gcagttaacg ttaagccagc aggtaaagct tatgttatgg ctaaatttag aggtacttta
    13681 tcaagtgtag agaatcatca gtacacgatt cataacttag aaagaccttt gtttaatgag
    13741 gctgaaggtg ctacactcaa aaactttaac ttaggtaatg taaatataaa catgccttgg
    13801 gctgataaag ttgcacctat tggtaatatg tttaagaagt ctacacttga gaatatcaaa
    13861 gtagtgggtt cagtaacagg aaataacgat gtaaccggtg ctgttaataa gttagacgaa
    13921 gctaatatgc gcaatgtagc ttttattggc aagattaaca gtcttggaga taaaggctgg
    13981 tggtctggtg gacttgtcag cgaaagttgg cgaagtaaca cggatagtgt ttattttgac
    14041 ggtgatattg taggtaacaa ttctaagttt ggtggtttag tagctaaagt caatcacgga
    14101 agtaatcagt gggatgttaa acaaaaaggt cgtttgacaa attctgttgt taaaggtact
    14161 atgactctga aaaatcatgg tcaaagtggt ggtttagttc acgaaaacta tgactggggt
    14221 tgggttgaga ataatattag tatgatgaaa gtcaataacg gtgaaattat gtatggttca
    14281 ggttctattg acggtgaccc atactttggt tttgactact tcaaaaataa ttattatgta
    14341 aaagatgttg ctactggaga aagtacctac aagcgttcga aacagattca gtctatctct
    14401 caagccgaag cagacgccaa aatcgctaat atggggatta ctgcgaatac cttcgctatt
    14461 caagatccag ttgtaaacaa gttaaatcgt atcattgata gagattctga atataaagcg
    14521 attcaggact accaagaaac aagaaaccta gcttatcgaa acttggaaaa acttcaaccg
    14581 ttctataata aagagtggat tgtaaaccaa gggaataagt taacagatga gtccaatctt
    14641 gttaagaaaa ccgttctgtc tgtaacgggt atgaaatcag ggcaatttgt tactgattta
    14701 tcaagtgtgg ataaaatcat gattcactat gccgatggaa ccaaagaaga atttggggta
    14761 tctgcgattt cggactctag ggtaaaacaa gtcaaagaat ataatgtaga tgatttaggt
    14821 gtagtttaca ctcctaatat ggttgataag aacagagatt ctttgattac gaaagttaaa
    14881 gagaagttga gttctgttgc tttagactct gctgaggtta aatctattac aaataaccct
    14941 gcatctctat acctagaaga aagtttcgct gaggttagag agacgttaga taagttagtg
    15001 aagtccttgt tagaaaatga agatcatcaa ctgaacagtg atgaagtggc tgagaaggcg
    15061 cttctcaaga aagttgaaga taataaagct aagattatat tagccttgac ttatctaaat
    15121 cgttattatg gtatcgacta tgatggcttg aactttaagc atttgatgat gtttaaacca
    15181 gacttttatg gtaaaacacc aagtatttta gatttcttga ttcgtatagg ttcagcagaa
    15241 aagaatttaa aaggagatag aagtttagaa gcttatcgtg aagttattgg tggtactatt
    15301 ggaaaagggg aattaaatgg cttgttaggt tataacatgc gtttattcac taagtacact
    15361 gacttaaatg attggtttat tcatgctgca aagaacgttt atgtttctga gcctgagacg
    15421 actacagagg atttcaaaga caagcgccat cgtatctatg acggtttgaa taatgatgtt
    15481 cacggtcgta tgattttacc gttattgaat ttgaaaaaag cgcatatttt tgtgatttcg
    15541 acttacaata caatagcctt tagttccttt gaaaaatatg gtaagaatac agaagaagag
    15601 cggaacgctt ataaagctga gattgatagg gttgcaaaag cacaacaacg ttacttagac
    15661 ttctggtctc gtttagcttt accgaaagtt cgcaatcaac ttttgaagag tcaaaactct
    15721 gtaccaactc ctgtttggga taatcaagtt tatgtaggtt taggtggtgc aaaccgtatg
    15781 ggttacggtg atgggggtcg agtagtaact ccagttcgtg aattattcgg cccgactgat
    15841 cgttggcacc aaataaactg gaatatggga gcgatggcga agatttatga acgtccttgg
    15901 aaagatgatc aagtttattt catggttaca aatatgatgg agccgttcgg tatctcagcc
    15961 tttacccatg aaactactca cgtcaatgat agaatggctt actacggtgg agattggcac
    16021 cgtgaaggta cggatttaga agcctttgct caaggcatgt tgcaaacacc tgataagtca
    16081 acaaccaatg gtgaatacgg tgctctcggt atcaatatgg cttatgagcg taaaaatgat
    16141 ggtgaacagc tttacaatta tgaccctgag aagttagata gtagagagaa aattgatagt
    16201 tacatgaaga actacaatga gtctatgatg atgcttgact acttagaagc tagtgcagtt
    16261 attcgtcaaa atttgtctga taattctaag tggttcaaga agatggataa agagtggcgc
    16321 actaatgcag atagaaaccg tttaattgga gaaccgcatc agtgggataa attacgtgat
    16381 ttaacagaag aagagaagaa gttacctatt gacagtattg acaagttggt agagaataac
    16441 tttgtaactc ttcatggtat gccgaagaac ggtcgttacc gcacagaagg ttttgatagc
    16501 tcttatcaac ctgtaaacat gatggcaggt gtctttggag ggaatacaag taaaagcacc
    16561 gtagggtcta tttcatttaa acataatgct ttccgtatgt ggggatacta tggatatgaa
    16621 aatggcttta ttccttatgt ttctaacaag ttaaaaggtg ctgcaaataa agagaataaa
    16681 gggcttttag gtgatgattt cattatcaag aaagtttcta agaatcaatt ccaaaatctc
    16741 gaagagtgga agaagcattg gtatcatgaa gtttatgata aagcacaaaa aggctttgtt
    16801 gagattgagg tagacggagt gaaaatttca acgtatgctc agttacaatc cttatttgaa
    16861 gaagctgtta gcaaagatct tgcaggtatg gatgataaga acattaagaa tcattaccaa
    16921 tatacagaga atctcaagtg gaaaatatac aaacaactcc ttaaaaacac agacggtttc
    16981 tctagtgatc tatttactgc accacaagca tagggatgaa
//
LOCUS       DAVKZB010000033        16706 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKZB010000033 DAVKZB010000000
VERSION     DAVKZB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.033"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..256
                     /locus_tag="VXA80_000977"
     CDS             <1..256
                     /locus_tag="VXA80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353493.1"
                     /translation="LIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLF
                     LGYLLVIQKAGYLFSVLLLFSNFSVPFIKAKEYELFKNSK"
     gene            1387..1626
                     /locus_tag="VXA80_000978"
     CDS             1387..1626
                     /locus_tag="VXA80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353494.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1611..1889
                     /locus_tag="VXA80_000979"
     CDS             1611..1889
                     /locus_tag="VXA80_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353495.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2175..4061
                     /gene="pspA"
                     /locus_tag="VXA80_000980"
     CDS             2175..4061
                     /gene="pspA"
                     /locus_tag="VXA80_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV0353496.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEVKAKFEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKAS
                     EEEQAANLKYQQAVLKYIKETDPQKRVEAQKVMDEAQKEHTRTKEKFDEVRAKVIPSE
                     EELKKTRQKAEEAKLKEAEVAKKVEETKKQEEEAKAKLEEAEKKATEAKQKVDAEEVA
                     PQAKIAELENQVHRLEQELKEIDESDSEDYIKEGFRAPLQSELDTKKAKLLKLEELSG
                     KIEELDAEIAELEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAEADLKKAVDEP
                     ETPAPAPAPAPAPAPAPAPAPAPAPAPKPAPAPKPAPAPKPAPAPKPAPAPAPAPAPK
                     PEKPAEKPAPAPKPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMA
                     TGWAKVNGLWYYLNSNGAMATGWLQYNDSWYYLNTNGAMATGWLEYNGSWYYLNANGA
                     MATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNAN
                     GSMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNGLGALAVNTTVDGYTVNEN
                     GEWV"
     gene            4461..5582
                     /gene="mnmA"
                     /locus_tag="VXA80_000981"
     CDS             4461..5582
                     /gene="mnmA"
                     /locus_tag="VXA80_000981"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV0353497.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5723..6178
                     /locus_tag="VXA80_000982"
     CDS             5723..6178
                     /locus_tag="VXA80_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0353498.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNESTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHREE"
     gene            6188..8101
                     /gene="mnmG"
                     /locus_tag="VXA80_000983"
     CDS             6188..8101
                     /gene="mnmG"
                     /locus_tag="VXA80_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV0353499.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNIKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(8308..8400)
                     /locus_tag="VXA80_000984"
                     /pseudo
     CDS             complement(8308..8400)
                     /locus_tag="VXA80_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8454..10133)
                     /locus_tag="VXA80_000985"
     CDS             complement(8454..10133)
                     /locus_tag="VXA80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0353500.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10135..10368)
                     /locus_tag="VXA80_000986"
     CDS             complement(10135..10368)
                     /locus_tag="VXA80_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV0353501.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11186..11338)
                     /gene="cibB"
                     /locus_tag="VXA80_000987"
     CDS             complement(11186..11338)
                     /gene="cibB"
                     /locus_tag="VXA80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV0353502.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11340..11525)
                     /gene="cibA"
                     /locus_tag="VXA80_000988"
     CDS             complement(11340..11525)
                     /gene="cibA"
                     /locus_tag="VXA80_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV0353503.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11703..12386
                     /gene="tsaB"
                     /locus_tag="VXA80_000989"
     CDS             11703..12386
                     /gene="tsaB"
                     /locus_tag="VXA80_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV0353504.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENSKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SVESYIKRL"
     gene            12383..12820
                     /gene="rimI"
                     /locus_tag="VXA80_000990"
     CDS             12383..12820
                     /gene="rimI"
                     /locus_tag="VXA80_000990"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV0353505.1"
                     /translation="MIEIKRIQQRPDLAQAIYAVMIAVYPINPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12810..13820
                     /gene="tsaD"
                     /locus_tag="VXA80_000991"
     CDS             12810..13820
                     /gene="tsaD"
                     /locus_tag="VXA80_000991"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV0353506.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWNLNAKPSLAFDT
                     ME"
     gene            complement(13860..15166)
                     /locus_tag="VXA80_000992"
                     /pseudo
     CDS             complement(13860..15166)
                     /locus_tag="VXA80_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15558..15638)
                     /locus_tag="VXA80_000993"
     CDS             complement(15558..15638)
                     /locus_tag="VXA80_000993"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0353507.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            complement(15872..16198)
                     /locus_tag="VXA80_000994"
     CDS             complement(15872..16198)
                     /locus_tag="VXA80_000994"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353508.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVETYFYLEYDRALSRQLVSL
                     EEDIII"
BASE COUNT         5012 a         3138 c         3759 g         4797 t
ORIGIN      
        1 tcttatcttt ttgataaaaa tttgtgtaga taccacgata aaatttttat tcatcagatt
       61 atttgacaca agtactttaa tgaagaggag aatatttttt cttttcgcat tggatgttat
      121 agcttactta tttttaggat atcttttagt gattcaaaaa gcaggctatt tgttttcggt
      181 tttacttctt ttttctaatt tttcagttcc atttatcaaa gcaaaggaat acgaattatt
      241 taaaaatagc aagtaataga aacataggtt aaatcttaga acggcttaaa atcagatatg
      301 ataaagggaa atagtcttat ctaggtaact aagtgctaca aacacagtcg aggatcactt
      361 gttaggatta cattttaatc atgcttaata agaatggaga attcctatgg gattgtatta
      421 ctctgaagtt tcgaatacct tagttctcgt gattttgagc tgttctagct ttagaatcct
      481 tcagaatgta aaattttaag cagtcaaaga cttagaatag cctgaaaaca tttattttat
      541 agcaattcta ggcttagaat tgctcatata tatgtatttg agctgctcga tagttaggac
      601 agctctattc tgagaaatct gagctatcaa tgcttcagaa tagtatttcg caaatggttt
      661 ttattgaaga ggtgattgtt ttatgatagt ctaaaataaa actgatttaa tggagtaaat
      721 ggagaatcgt cacatgaaac atttttttgc aggaattggt gagataaaac ttgttggcta
      781 cttattgtat atatgtgtag gactagcccc tctttttcat atatatgtca taggttcgga
      841 actgagtttt gtgaaaatag tgttatctat tttgggagtt atatttgtaa gtatgttaac
      901 tattgctcga atttatcgga cttttctata aagaatagtt tggacttgaa ctgtatctaa
      961 aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt gaaaagagtg agatatattg
     1021 acttttgaaa aaaatgtcat aattgagttg ggataaacct aaaagtaata tagtagattg
     1081 aatctggaat agtccgttat gactgttaaa atatttctag aaattaattt gactttccta
     1141 atcgatttgt tcacatcttg tttcaatcta ctatactttc tcgttaaacc gttcggcaat
     1201 tcatggtgac atggatacat agtagtaagc acactatgtg gcttggcaga gctaaaaact
     1261 gttcccttgc gataagccta ataagcacaa catagggagt tagagaagcc gactctaatc
     1321 aaccactttg ggcagtagtg agaactgccc cgtgcttttt attttgagaa aatatggagt
     1381 ttgtcgttga aattacttga ttgtattttg gactatcaag aaagattcaa tggaaaaaca
     1441 tgtcaagtat caacgaatta taagtattta gagattttca aagtgaattt ttgcttgact
     1501 gatttacatc atttatttga cttatacaaa atcacacgag attatgctag tcaaacaaaa
     1561 cctgctattc aagctggtgt ttttatttta gaagatttta gaaatatcct atgtacaatg
     1621 atgtaatcga aaggatatct ttatatgagt ttatcggtga tattttctat tctaaaataa
     1681 taagttgttg catcgtagct agggatttat ctaaaaatac tatgaaattg gacgtcatat
     1741 tttttgagga taaaaataaa agatccgcag ttttaggttt acgaagagac aaaagcggag
     1801 tatttaaacc agttactcta cattttacaa gcgctaagaa atatgctaaa gttcgtaaaa
     1861 cagatgtgaa agaaatgaaa tggctataaa gattgtccgc aggcttaagc ttgcgttttt
     1921 tgttgtttaa cgaaattaaa aaacaagctc tctcatcgga aatgttttta taggaatgaa
     1981 ggaagatgat gctatgttta aaaagagaat cgaaaagggc aaaagtagtg tctttatctt
     2041 tttggagtag aagcttatga tatagaaatt tgtaataaaa atgtaatata aaacatttga
     2101 caaatattta cggggggagt atacttaata taagtatagt ctaaaaatga ctatcagaaa
     2161 agaggtaaat ttagatgaat aagaaaaaaa tgattttaac aagcctagcc agcgtcgcta
     2221 tcttaggggc tggttttgtt gcgtctcagc ctacttttgt aagagcagaa gaagctcccg
     2281 tagctagtca gtctaaagct gagaaagact atgatgcagc agtgaaaaaa tctgaagctg
     2341 ctaagaaaca ttacgaagaa gtaaaagcaa aatttgaagc tgctaagaag gaatatgagg
     2401 acggaaaagc tgctcagaaa aagtatgaag atgatcagaa gaaaactgag gagaaagcgg
     2461 aggaagaaag aaaagcttct gaagaagaac aagctgcaaa tctgaaatat caacaagcgg
     2521 tgcttaaata tataaaggaa actgatcccc aaaaaagagt tgaagctcag aaagtaatgg
     2581 acgaagctca gaaagagcat acaagaacaa aagaaaaatt tgacgaagtt cgcgcaaaag
     2641 taattcctag cgaggaagaa ttaaaaaaga ctagacaaaa agcagaagaa gctaaattaa
     2701 aagaagctga agttgctaaa aaagtagaag aaacgaaaaa acaagaagaa gaagctaaag
     2761 caaaattaga agaggctgag aaaaaagcta ctgaagccaa acaaaaagtg gatgctgaag
     2821 aagtcgctcc tcaagctaaa atcgctgaat tggaaaatca agttcataga ctagaacaag
     2881 agctcaaaga gattgatgaa tctgattcag aagattatat caaagaaggt ttccgtgctc
     2941 ctcttcaatc tgaattggat accaaaaaag ctaaactatt gaaacttgaa gagttgagtg
     3001 gtaagattga agagttagac gctgaaattg cagaacttga agttcaactt aaagatgctg
     3061 aaggaaacaa taatgtagaa gcctacttta aagaaggttt agagaaaact actgctgaga
     3121 aaaaagctga attagaaaaa gctgaagctg accttaagaa agcagttgat gagccagaaa
     3181 ctccagctcc ggctccagcc ccagctccgg ctccagcccc agctccagct ccagctccag
     3241 ctccagctcc ggctcctaaa ccagctccag ctcctaaacc agctccagct cctaaaccag
     3301 ctccagctcc taaaccagct ccggctccag ccccagctcc ggctcctaaa ccagaaaagc
     3361 cagcagaaaa accagctcca gctcctaaac cagaaactcc aaaaacaggc tggaaacaag
     3421 aaaatggtat gtggtacttc tacaatacgg atggttcaat ggcaacaggc tggctacaaa
     3481 ataatggctc atggtactac ctcaacgcta atggtgctat ggcaacaggt tgggctaaag
     3541 tcaacggctt atggtattac ctcaacagca atggcgctat ggcgacagga tggctccaat
     3601 acaatgattc atggtactac ctcaatacta atggtgctat ggcgacaggt tggctagaat
     3661 acaatggttc atggtactac ctcaacgcta atggtgctat ggcgacaggt tggctagaat
     3721 acaatggctc atggtactac ctcaacagca atggcgctat ggcgacaggt tggctccaat
     3781 acaatggttc atggtactat ctaaacgcta acggcgctat ggcaacaggt tgggctaaag
     3841 tcaacggttc atggtactac ctcaacgcta acggttcaat ggcaacaggt tgggtgaaag
     3901 atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaaac caatggttca
     3961 aagtatcaga taaatggtac tatgtcaatg gcttaggtgc ccttgcagtc aacacaactg
     4021 tagacggata tactgtcaat gaaaacggtg aatgggttta agccgattaa attaaatcat
     4081 gttaagaaca tttgacattt taattttgaa acaaagataa ggttcgattg aatagattta
     4141 tgttcgtatt ctttaggtac ctgtcttatg atttcaggaa atgtcattaa aaaaacgact
     4201 ccttttctct aacttggaaa atagattaga gaaaatgggt tgttttattt attatagtta
     4261 tttgaatgaa gataagaaga agatatactc acatcattca caaatctgta tattgactat
     4321 aagttttaaa aaacaatttt taagctcttc cttgtcttct ctaactaagc gtgttataat
     4381 gaatactgct caagcgacct tcaatcgtga agcacacacg accttcaatc gtgaataaac
     4441 gaatagatgg gagacttacc atgagtgata actctaaaac acgtgttgtc gtggggatga
     4501 gtggtggtgt tgattcgtcg gtgacggctc ttcttctcaa ggagcagggc tacgatgtga
     4561 tcggtatctt catgaagaac tgggatgaca cagatgaaaa cggcgtctgt acggcgaccg
     4621 aagattacaa ggatgtggct gcggtggcag accagattgg cattccctac tactctgtca
     4681 attttgaaaa agagtactgg gaccgcgttt ttgagtattt cctagcggaa taccgtgcag
     4741 ggcgcacgcc aaatccggac gttatgtgca acaaggaaat caagtttaag gcctttttgg
     4801 actatgccat aaccttgggg gcagactatg tagcgactgg gcattatgct cgagtggcgc
     4861 gtgatgagga cggcactgtt cacatgcttc gtggcgtgga caatggcaag gaccagacct
     4921 atttcctcag ccaactttcg caagaacaac ttcaaaaaac catgttccca ctaggacatt
     4981 tggaaaagcc tgaagttcga aaactagcag aagaagcagg cctttcgact gctaagaaga
     5041 aagactcgac agggatttgc tttatcggag aaaagaactt taaaaacttt ctcagcaact
     5101 acctgccagc tcagcctggt cgtatgatga ctgtggatgg tcgtgatatg ggcgagcatg
     5161 ctggtctgat gtactatacg attggtcagc gtggcgggct tggtatcggt ggacaacacg
     5221 gcggtgacaa tgccccttgg ttcgttgtcg gaaaagatct aagcaagaat attctctatg
     5281 taggacaagg attctaccat gattcgctca tgtcaactag cctagaagcc agtcaagtcc
     5341 actttactcg tgaaatgcca gaagagttta cgctagaatg tacggctaaa ttccgttacc
     5401 gtcagcctga ctctaaggtg accgtccatg tcaaaggaga caaggcagaa gttatctttg
     5461 cggaaccaca acgcgcgatt acaccaggac aggcagttgt cttttacgat ggcgaagagt
     5521 gtctaggtgg cggtttgatt gacaatgctt accgcgatgg acaagtttgt cagtacattt
     5581 agattgacaa attttctcaa tttgctacaa taataacagc aatagaaatg atggtcaaag
     5641 ctcatggatg ttgcaggctt ttttgtcctg cacttctttg tagttttgac tgtttttgtg
     5701 tcgtttaagg gaaaggacaa gaatgactca acaagacttt cggacaaaag tagacaatac
     5761 ggtttttggt gttcgggcga cagctttgat tcttcaaaat cgcaagcttc tagtcaccaa
     5821 agacaagggc aagtattaca ttatcggcgg tgcgattcaa gtcaatgaaa gcacggaaga
     5881 cgcggtagtc cgtgaagtga aggaagaact gggtgttaaa tctcaagctg ggcagctagc
     5941 ttttgtggtt gaaaatcgtt ttgaagtgga cggcgtttcc tatcacaata tcgagtttca
     6001 ttatctggtg gatttgctgg aggatgcccc attgaccatg caggaagatg agaaaaggca
     6061 gccctgtgag tggattgact tggataagct tgaggatatc cagctagttc cagccttttt
     6121 aaaaacggcc ctaccagatt gggaaggcca actaagacac attcatcgtg aggaatagga
     6181 gagaaacatg acttatcatt ttactgaaga atacgatatt attgtaattg gtgcgggaca
     6241 cgctggggtt gaggcttcct tggccgctag ccgtatgggc tgtaaggttc tacttgcgac
     6301 tatcaatatt gaaatgctgg ctttcatgcc ttgtaatccc tctatcggtg gttctgctaa
     6361 ggggattgtc gtacgtgaag tcgatgccct cggtggcgag atggccaaga ccattgacaa
     6421 gacttacatc cagatgaaga tgctcaacac agggaagggt cctgctgttc gtgcccttcg
     6481 tgcgcaggct gataaggaac tttactctaa ggaaatgcgc aagacagttg aaaatcaaga
     6541 aaatctgacc cttcgtcaaa ccatgattga tgagattttg gtggaagatg gcaaggttgt
     6601 cggtgtgcgt acagccaccc atcaagaata tgctgctaag gctgttattg tgacgacagg
     6661 gactgctctc cgtggggaaa ttatcatcgg agacctcaag tactcatcag gtcctaacca
     6721 cagcttggct tctattaacc tagctgacaa tatcaaggaa ctgggcctcg aaatcggtcg
     6781 tttcaagaca ggaacccctc cacgtgtcaa ggcctcttct atcaactacg acgtgacaga
     6841 aatccagcca ggagacgaag tgcctaatca tttctcatac acttcacgtg atgaggatta
     6901 tgtcaaagat caagtgccat gctggttgac ctataccaat ggtaccagtc atgagattat
     6961 tcaaaacaac ctccaccgtg cgcctatgtt tacaggtgtg gtcaagggag tggggcctcg
     7021 ttactgtccg tcgattgaag acaagattgt gcgctttgcg gacaaggaac gtcaccaact
     7081 cttccttgag ccagaagggc gcaatactga ggaagtctat gtgcaaggac tttcaaccag
     7141 tctgcctgag gatgtccagc gtgacttggt tcattccatc aaaggtttgg aaaatgcaga
     7201 gatgatgcgg acaggttatg ctattgagta tgatatggtc ttgcctcatc agttgcgtgc
     7261 gactttggaa accaagaaaa tctcaggtct cttcactgct ggtcagacaa atggaacatc
     7321 aggttatgaa gaagctgctg gccaagggat tatcgcgggt atcaatgcgg ctctgaaaat
     7381 ccaaggtaaa cctgagttga ttttgaagcg cagtgatggt tatatagggg tgatgattga
     7441 tgacttagtg accaagggaa ccattgaacc ttaccgtctc ttgaccagtc gtgctgaata
     7501 ccgtctcatt cttcgccatg acaatgccga tatgcgtttg acagagatgg gccgtgagat
     7561 tggccttgtg gatgatgaac gctgggctcg ttttgaaatc aagaaaaatc aatttgataa
     7621 tgagatgaaa cgcctagaca gtatcaaact caagccagtc aaggaaacca atgctaaggt
     7681 tgaggaaatg ggcttcaagc cgttgacaga tgcggtgaca gccaaggaat tccttcgccg
     7741 tccagaagtt tcttaccaag atgtggtggc cttcatcgga ccagctgcag aagacttgga
     7801 tgacaagatt atcgaattga ttgaaacaga aatcaagtac gaaggctata tttccaaagc
     7861 catggatcag gttgccaaga tgaaacgtat ggaagaaaaa cgcattccag ccaatattga
     7921 ctgggatgac attgactcta tcgcaacaga agctcgtcag aagttcaaac tcatcaatcc
     7981 agaaaccatc ggccaagcca gccgtatttc gggagtaaac ccagcagata tttctatttt
     8041 gatggtgtat ctggaaggta aaaatcgtag tatttctaaa actcttcaaa aatcaaaatg
     8101 atacgtcgtc ggcttcttac gaatgagttc aaagcttggc tttgattcat ctccaacctc
     8161 ccatagttcc ccgaactatg ggagctaact caagttatgc tttcgcttag ccttctagtc
     8221 tgatttcgat ttttattgat actcttcgaa aatctcttca aaccgcgtca acgtcgcctt
     8281 gccgtaggta tggctactga cttcgtcagt tctatctaca acctcaaagc agtgctttga
     8341 gcaacctacg gctagcttcc tagtttgctc tttgattttc attgagtatg agttttaatc
     8401 tcaaaagaaa gcatagagaa aaacagctcc gaggacgggg ctgttttctt gctttattct
     8461 tcatctggtg tgaggatcat cgggatgatg atcggttcac gttcggtatt ttcatagagg
     8521 aaggggcgaa tagcgttgac aatggcacca ttgacagatt gcacgctagc atccttattt
     8581 ttcagtgcga tacgaatggc attgaagagg atacgctggc tttggcgaat caagtcgcca
     8641 gactctctca tgtagacaaa gcctcggctg aggatgtctg gaccagatag aatcatctgc
     8701 gatttgaagt caacagttgc aactgccaga acgacaccgt cttcagatag atcgcgacga
     8761 tctttgagga cagctgcgcc aatttcaccg atacgatttc catcgacata gatatcttgg
     8821 gcgttgaaat gacctgcgat acgagctgag tcagcagtaa gggcaagcac atcgccattg
     8881 ctcatgataa agatattgtc cttctcaaca ccagtatcca ctgctagtcc agcgtggact
     8941 ttttgcatgc ggtattcacc gtggacaggc atgaagtatt ttggcttaat caagcagagc
     9001 atgagttttt gctcttgctg accaccgtgt ccagatgtat ggatattgtt cactttaccg
     9061 tggataactt cgacaccagc ttcagaaatg atgttaatca gcttgttgac gctagtagtg
     9121 tttccaggga tgggactaga agagaagata acggtatcac ctggttgtaa ttgtacttga
     9181 cggtgggttc cgttggcgat acgagagagg gctgccatag gctcaccctg actacctgta
     9241 cagaggataa gaacttctcc tgcaggataa tctttgattt catttggctc gataaaggtt
     9301 cccttaggag ctttgatgta gccaagatcg attccgttga caatggcctt ttccatagaa
     9361 cgaccaaaga ccgcaatctt gcgtccagtc ttaacagcag cttctgttgc ctgctggaga
     9421 cggaagatat ttgaggcaaa ggatgcaaag atgatacgtc cttcaatacc ttggataatc
     9481 ttcataatgg actgaccaac gactttttca gagttggtaa aggttggtac ttccgcattt
     9541 gtcgagtcag acaggagaca gagcacgcct tcttcaccaa gcgcagccat acgatgcaag
     9601 tccgcaggtt ctccaactgg agtaaagtcg aacttaaagt cacccgtaca gacgattttc
     9661 ccttgaggag tatgaatgac aatccccaaa ggctctggaa tagagtgagt cgttctaaag
     9721 aaagttgcct tgagattttt aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg
     9781 gcgttgcgca agaggccgtg ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt
     9841 ccagcgtaga tagggacatt tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc
     9901 tcgtgtccgt gtgtgattaa aacagccttg acgcggtcga tattgtccac gatgtaagag
     9961 tagtcaggaa tgacatagtc gataccaagc aagtcatctt ctgggaattt aatcccagca
    10021 tcgacgatga taatctcgtc ttggtattca attccgtaag tgtttttccc gatttctccc
    10081 agaccaccga tggcaaaaac gccgacttct tcaggtttaa gagtataggc catattagaa
    10141 ctccgtgatt tcgaaggcgc cagtttcttt ttcgtaatcg agcaatttgt cagacaagag
    10201 ttcgatatat tcgatattgt actctgggcg attttcttcg acaagttggc gagcagtgat
    10261 gcgaccctca agttctgagc tggcatcgat gtctaggtaa agtgcgcgtg ttgtttcacg
    10321 gcgtgggcta cgttcttttg tttcttgata aaaaactttg taaatcatat agttcctttc
    10381 tatttacagg tcagcttatt ccaaactttt agcagagatt tgcttgattt cattccattt
    10441 tgtgtctaga ttgaccttga ttcgttacaa ttatttcaat tataccacaa aatgaaaaat
    10501 tagtaaaaga cggaaacgag aaagttaggg gacgatttct tcggtaagcg tttgcaaaaa
    10561 taaagaaaat gtgttatgtt gataacggaa gtgaagttta ttacttgtca taattccata
    10621 tagattttaa gtcaaggagg ttgccctatg tataacttat ttgcttttgc tatcggagtt
    10681 cgtttgttag cttttatcgt ttttgttgct tgcgtttatg gaggaaatct tctatttgcc
    10741 aaactggaac gatgacaaac caagttcttg tggaaaatta cctttgtgac tctctactcg
    10801 ctttttctcc tcctcgtgac ctatgtcgtt tggtttgtat tttactttgg attaaatgct
    10861 tagatgccct gcttatgctg gggttttttg tttggggatg tagagatagt agatttacga
    10921 cacgaaaatt ccaccccatc tatcaagatg aggtggtctt tgcattttta taaaatgaca
    10981 tgacaatgga attttcaaag cttattattt ttatgggagt cattgttctg ctcaaaaata
    11041 gagttgaagc ataaaaataa aacaatacaa acagataaat cgaatggtaa agggatagta
    11101 gtaatgttca aatcattgag aatgctgagc aaactgtaag agatgaagac aataaaattt
    11161 atccttctaa aaatgtgttt cttatctagc atgacttacc aaacttttta cgaaggtctt
    11221 ttccaatggt ataacctgcc ataaataaag cgataccaac acctacacca aagccaccct
    11281 tgatttcttg caattcctta ttagaaattt cacgatagtt gttcaaattt ttcatcatat
    11341 taatctcctc ctaaaaaata accagctgcc aaaccagaac ctataagtgc tgttccacct
    11401 gcaatagcct tccaagatac tgctactcca aagataacca agggagcgag accgccatca
    11461 atatctgata attcattttc agataaggat aaaaattgat tgtcaagaat gtcaaaattt
    11521 gtcattgaac tttcctcctt ttttcttgtt cacttttata ttcggaaaag aaaaggaaaa
    11581 gtgagcattt ttttaaatta tttttagtta agtgagggca ctcttgtctg ggggcaaaga
    11641 aaattcccat gtgccagctt ttgtagtata atagtaagca gacaaaaaga taggaatgtg
    11701 ttatgaaagt attagctttt gatacgtcca gcaaggctct ttctctcgct attttagagg
    11761 acaagcaggt tcttgccgag acgacgatta atattaagaa aaatcacagt attactctta
    11821 tgcctgccat agattttttg atggcaagtt tggattggac acccaaggat ttggaccgaa
    11881 tcgtggtagc tgaagggccg ggtagctata caggcttgcg aattgcggta gcaactgcta
    11941 agaccttagc tcacaccctg aacatcgagt tggttggtat gtcgagtctc ttggctctgg
    12001 tgccccacca acaagaaggt ttgtttgtcc ccttgatgga tgcgcgtcgc aataatgttt
    12061 atgcaggatt ttatgaaaat tccaaacctg tcatggcaga agcacatctg ccttttgagc
    12121 gagtcattga gctaatcaag ggcgctagtc aggtaacctt tgtcggagaa gttggcccct
    12181 ttgttgagca gattcaaaaa cacttgccaa ggactgatta caaagaaaca ttgccaaatg
    12241 cagctaatct agctctatgg gcctgggaca aggaagcaga ctccttgcat gattttgtgc
    12301 cgaactacct caagcgtgtc gaagctgagg aaaactggct caagaatcac accgagtctg
    12361 tcgagtctta cattaaacgc ctatgataga aatcaaacga atccaacagc gacctgactt
    12421 ggctcaagcc atctacgctg ttatgatagc tgtttaccca atcaaccctt ggacgctgga
    12481 acaaatccaa gcagatctgt cccaagacca gacttggtat gcattggctt atgatggggc
    12541 agaagtgatt ggatttctag ctgtgcagga gaatcttttt gaagcagaag tcctgcaaat
    12601 cgctgtcaaa ggagcttatc agggtcaggg aattgcgtca gccttgtttg ctcaattgcc
    12661 gacagacaag gaaattttcc tcgaagtcag acagtcaaat caacgagcgc aagcatttta
    12721 caagaaagaa aagatggcag ttatcgctga gcgaaaggcc tactaccatg acccagtcga
    12781 ggacgccatt atcatgaaga gagaaataga tgaaggatag atatatttta gcatttgaga
    12841 catcctgtga tgagaccagt gtcgccgtct tgaaaaacga cgatgagctc ttgtccaatg
    12901 tcattgctag tcaaattgag agtcacaaac gttttggtgg cgtagtgccc gaagtagcca
    12961 gtcgtcacca tgtcgaggtc attacagcct gtatcgagga ggcattggca gaagcaggga
    13021 ttaccgaaga ggacgtgaca gctgttgcgg ttacctacgg accaggcttg gtcggagcct
    13081 tgctagttgg tttgtcagct gccaaggcct ttgcttgggc tcacggactt ccactgattc
    13141 ctgttaatca catggctggg cacctcatgg cagctcagag tgtggagcct ttggagtttc
    13201 ccttgctagc ccttttagtc agtggtgggc acacagagtt ggtctatgtt tctgaggctg
    13261 gcgattacaa gattgttggg gaaacgcgag atgacgcggt tggtgaggcc tatgataagg
    13321 tcggtcgtgt catgggcttg acctatcctg caggtcgtga gattgacgag ctggctcatc
    13381 aggggcagga tatttatgat ttcccccgtg ccatgattaa ggaagataat ctggagttct
    13441 ccttctcagg tttgaaatct gcctttatca atcttcatca caatgccgag caaaagggag
    13501 aaagtctgtc tacagaggat ttgtgtgctt ccttccaagc agctgtactg gatattctca
    13561 tggcaaaaac caagaaggct ttggagaaat atcctgttaa aaccctggtt gtggcaggtg
    13621 gtgtggcagc caataaaggt ctcagagaac gcctagcagc cgaggttaca gatgtcaagg
    13681 tcatcattcc acctctgcgc ctctgcggag acaatgcagg tatgattgct tatgccagtg
    13741 tcagcgagtg gaacaaagaa aacttcgcag gctggaacct caatgccaaa ccaagtcttg
    13801 cctttgatac catggaataa agagtggctc tttgtcaagt gtagtgggta ggcgaaaagc
    13861 tacaatctgg agagtacgaa attcgtactc tcttttttga tgttcaaagt catcaaaatc
    13921 cgttttttaa aattgttaaa gttccggaaa ccaaaggctt tgcgcttgat aaccttaatc
    13981 aaattattgg tcgcttcaag tttagcgttg gaataaggca gcttaagtgc gttgataatc
    14041 ttatctctat cccttaaaaa agtccaaaag accgtttgaa aaagcggatg aaccgttggc
    14101 agttcttgct caatcaatcc aaagaaatgg tcggcattct tctcttgaaa atgaaagagc
    14161 aggagttgat agagttcgta gtgaacctgt aatccctcag agtaagaaag cagcttatct
    14221 cgtacttccc gatgggttaa gtgcattcga aacgtgggct gatagaatcg tttctgacag
    14281 agcttgaagc tatcctggtg tagtaaatca gtccagtgga ctgatttagc ccgagcctag
    14341 aaacgaaaaa gcgagggttc caaaagcgct tgagcgcccg atactccaag gattttcggt
    14401 caaattggtt catgatttgg attcgtactc ggttcatagc tcggctcaga tgttagacaa
    14461 tgtggaagcg gtcaagaaca atcttcgcct ttggaaagag ttgcttagcg agccgataat
    14521 aagggctgta catgtccatg gtgatgacct cgaccagctc ccgaacctct ctctgatagc
    14581 gttggaaatg gtttcgaatc accgcatgag ttcgcccgtc taaaattgcg aggatggatt
    14641 tggactcaaa atcttgggca atgaagctca ttttgctctt tttgaagcta tactcatccc
    14701 aactcataac ttttggcaac ttggtccaat cggtttcaaa cttaaattcc ctcagttttc
    14761 ggatgacggt ggaagttgag cccgccaatc tgtgggcaat ctcagtcata gactgctttt
    14821 caaggaggag ctgagcgatt ttttgtctca ccatgttgga aatctggcaa tttttcttga
    14881 caatggatgt ttgagaaacg accaccttaa ggcaattctt gcactgaaag cggcgctttt
    14941 tgagatgcag taccgtgggt aaaccctgac agtcgagaag cggaatttta gaggctttct
    15001 ggaagtcgta tttgatcatc ttcccttgac aatgaggaca aggaggagcg ggggaatcca
    15061 actttgcctg aacgactaga tgggtttggt atttcagaac agacaagatt ttgatatttt
    15121 tgtcttccaa tccgagcaaa tctgtggtat tcttaagttg ttccatatga accctttcta
    15181 atgagttggt tagacgcttt tcattatagg tcatatgggg ctttttttct acaagaaacg
    15241 accctataat tcctggggtg ggattaccca ctacagaaat tatagagcca aaacattcca
    15301 aagtcttgtc tgactctgga atgttttctg ttcacagttg ttttgtaact gcctaataag
    15361 taaccgcgct ataacgaggt atctaccgag attgtcaatt ctccagtata ctgttataaa
    15421 aaacaaaact ggaggatttt tttatgtccc cccatcgtcc catcgactgt tccgtagctg
    15481 cccaataacg gagtatagtc gtctgaaaca agaataggac aaaagtgcct caagaaaagc
    15541 atcgtaattt gacaatatta ttctgagatg tttttgatgt aagcccatat tttctcaatg
    15601 ggattatact cgggtgaata ggtaggaagt ggtaacagtc tatgccctgc tccttacaca
    15661 tgttcattct gtgaaacctt gcattgtcca taatgataag ggatggtgta tctaaagtag
    15721 gtagtaagaa tttttggaag caagcttcga aaaagtcact cgtcatcgta tctttatagg
    15781 tcatcggagc catagtatat tgaaacaaaa agggcattag tacttctata acagtatcgc
    15841 aatctctggt gtgcgttgtc tcgatcttaa ttcaaataat tatatcttct tccagagaga
    15901 ctaactgcct gctcaaggct cgatcatatt cgagataaaa ataggtctca acccctgtct
    15961 cataaatata aataggagtc aggtagctta agtgattcaa ttctttaagg aaccgattta
    16021 ctttttctgg gtcttgttcg cagtaggtac ggctcttttt tagactatat cccatagcct
    16081 ttagagcgta atgaatagtt gttggaggac agttgaattc agcagctatt tcagtcaaat
    16141 aagcatcggg atgagtttca atatagatct ttaatttatc tctatcaact ttttcaatta
    16201 atagtggaag ttttcttaaa gttagtgtat tgaaacgttt ttatacagtg ctaaaatttg
    16261 aagtgtagtg cagatgtatt tgtaatagta ttatagcaat tctttggaga aggtcgagac
    16321 aagagaaaat aggtatcttt gagatataag catgatttac tataaaatta ttgagttatt
    16381 cataaaaatt tctctatcaa atatcataat gcgtcgttta aggagatggt tacaatgcaa
    16441 tatagttgtg gaaaaataaa tattaatatc cctgatggat atggagacat taaggatata
    16501 gtgttttcgg ctcatattat agttaggtat aataatggac attgtggagg tatagatccg
    16561 catataattg gactttgtaa aaagcagatc agaaggatgt ctctatatcc tattttgata
    16621 atcgtatcta gagattcaaa ggttattgat gattataaaa atttagatat tgcctatgtt
    16681 gattgtactc aatgttcaaa taattt
//
LOCUS       DAVKZB010000034        16482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKZB010000034 DAVKZB010000000
VERSION     DAVKZB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.034"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            104..1882
                     /locus_tag="VXA80_000995"
     CDS             104..1882
                     /locus_tag="VXA80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV0353509.1"
                     /translation="MSIDEYVMGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILSGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNTAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNEQKDQAVADDNEGDENDDADY"
     gene            1869..3185
                     /locus_tag="VXA80_000996"
     CDS             1869..3185
                     /locus_tag="VXA80_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV0353510.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEESFKAIIDEEVGRK"
     gene            3360..4346
                     /locus_tag="VXA80_000997"
     CDS             3360..4346
                     /locus_tag="VXA80_000997"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV0353511.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4410..4481
                     /locus_tag="VXA80_000998"
     tRNA            4410..4481
                     /locus_tag="VXA80_000998"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4443..4445,aa:Arg,seq:ccg)
     gene            4664..5362
                     /locus_tag="VXA80_000999"
     CDS             4664..5362
                     /locus_tag="VXA80_000999"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV0353512.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5353..8892
                     /gene="smc"
                     /locus_tag="VXA80_001000"
     CDS             5353..8892
                     /gene="smc"
                     /locus_tag="VXA80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV0353513.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8889..9683
                     /locus_tag="VXA80_001001"
     CDS             8889..9683
                     /locus_tag="VXA80_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0353514.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9683..10501
                     /locus_tag="VXA80_001002"
     CDS             9683..10501
                     /locus_tag="VXA80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0353515.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10505..11782
                     /gene="ftsY"
                     /locus_tag="VXA80_001003"
     CDS             10505..11782
                     /gene="ftsY"
                     /locus_tag="VXA80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV0353516.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11828..13315)
                     /locus_tag="VXA80_001004"
     CDS             complement(11828..13315)
                     /locus_tag="VXA80_001004"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV0353517.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(13464..14204)
                     /locus_tag="VXA80_001005"
     CDS             complement(13464..14204)
                     /locus_tag="VXA80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0353518.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14204..16369)
                     /locus_tag="VXA80_001006"
     CDS             complement(14204..16369)
                     /locus_tag="VXA80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0353519.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5152 a         2924 c         3640 g         4766 t
ORIGIN      
        1 aaataaagaa gagttattaa ataaggcaac tgttggtgaa aagtttaatg ataaattaaa
       61 agaagagttt ctccaggaat ggcctttgga taggatttta acaatgtcaa tcgatgaata
      121 tgtaatggga aagggacagc aaaataagtc tttatgctac gctcttgaga agggaaaata
      181 caaaaatcta tttcttggaa tttctggtgg ctcagcttca aaatttggta tttattggaa
      241 taaaaaaaca aacaaatata aagatcaagc taataatgag atttcagagt tggatcagcg
      301 attttcaaaa ttaaaatcag atttgtatga aattatcaaa gaaggtattc gttttaactt
      361 tgaaaatcct atttttgata tgaaaagatc aacaaatgaa tttattggtc gttctgctat
      421 ggtgacaaaa ttactttgta tctattctga gggagatcct ttctttggtg taaatattaa
      481 tagtcagaaa gaattttgga accactttgt ttctcagaca aatcaaggtg gaccttatct
      541 gcaaaatcat aaaataattg aactggtgtc caaaacttat cctgagttgg agccatcgaa
      601 attaggaact atgctttttg agtattctaa gctttttatg gaaaataagg aagacaatag
      661 tacaatggat tcatcaaaca attttcgtca tcaattaact caatctctat taaagtctcc
      721 aaaccttatc cttagtggtg ctcctggcac aggaaagact tatcttgcta aagaaattgc
      781 caaagaatta acggatggca acgaagacca aatcggattt gtacaatttc acccatcata
      841 tgattatacg gattttgtag aaggtttaag accagtatca aatggggatg gagctattga
      901 gtttaggcta caggacggta tttttaaaga tttttgtcag aaagcaaaag aaacccaatt
      961 gattggagga caagataatt ttgatgaggc ttgggattct tacttagaat atataaatgt
     1021 tgctgaagaa aaagaatata taacaaaaac atcttactta tctgttaata gtagacaaaa
     1081 tttgtcagta aattatgata gtggtgttcc aggatggtca ctacctagca aatatgttta
     1141 cgagttgtat aaagataaaa attataataa gcaagaatac tacaaaagtg gtggaaaaac
     1201 tgtcctagaa acattgagaa agagatttgg tttgaaagac tatgtttccc caacagaaat
     1261 tgatactgat aagaaattcg tcttcatcat cgatgaaatc aatcgtgggg agatttctaa
     1321 gatttttggc gaactctttt tctctatcga ccctggctat cgtggtgaaa aaggaagtgt
     1381 ttctacccaa tatgcaaatc tacacgaaac tgatgaaaag ttctatatcc ccgaaaatgt
     1441 ttacatcatc ggaactatga atgatattga tcgttcagtg gatacctttg attttgctat
     1501 gcgtcgtcgt tttcgttttg ttgaagttac tgccgagggt caagttggca tgttggataa
     1561 agagctgaat atccatgcag aagaagcaaa aattcgtcta agaaacttga acactgctat
     1621 cgaaaatgtt caggaattaa acagtcatta tcatattgga ccaagttatt ttcttaagtt
     1681 gaaggatgta gattttgact atgaattact ctggtctgat tatattaagc ctctcctaga
     1741 agactacttg cgaggttctt atgatgaggt tgaaactttg gaaactttga aaaaagcatt
     1801 tgaactgaca aataatgagc aaaaagatca ggcagtagct gatgacaatg aaggtgatga
     1861 aaacgatgat gcggattact gataatcaac acaagattat taaagaaaaa tttgttgaag
     1921 aatatcctaa actaagcaat cttcttttag acagaacctt ggaaagtcta tcccaagatg
     1981 aacgtatttt catttttcca aatgatttga ctcatactcc tgatttggat aaggaccaaa
     2041 agatttttga aacagtcaat cagaaaatca agacaggaaa tgtgattggt tttcttggat
     2101 atggtcagga aagattaacg atttcctcac gattttctga tgagagtaat gaccattttt
     2161 tacattatct cttaaacaag gttcttcata tcaatctcac tagtttagat gttgctttgt
     2221 ctcgtgaaga gaggctttat caacttttga tgtatctctt ccccaagtat ctacaagctg
     2281 ctattcgaaa aggtctttat aaggaatatc atcgattttc tcataacgac agtcatgtta
     2341 agggagtgat tgatgtaaga aaccatctca agaaaaatct tcctttcacg ggaaatattg
     2401 cctacacaac gagagagttc acctatgata atcccctcat gcagttggtc cgtcacacta
     2461 ttgaatacat taagaatcag aaaagcattg gtcaaggggt actagataat ctctcaacta
     2521 gtcgtgaaaa cgtatctgaa atcgtgcgtg taacgccctc ttataaacta gctgatcgtg
     2581 ctaagattac tcggggaaat caatctaaac ctatacgtca tgcatacttt cacgagtaca
     2641 gaaacttaca agaactttgt ctgatgatcc taaaccaaga aaagcacggt ttagggtatc
     2701 aagatcaaaa aatctatggt attctctttg atgttgcctg gctttgggaa gagtatgttt
     2761 acaccttgtt gccaaaaggt tttgtacatc ccagaaataa ggataagacg gatggaattt
     2821 cagtattttc tgttgggaaa cgaaaagtat atccagattt ttatgacaga gaacgaaaga
     2881 ttgttctaga tgcaaaatat aaaaaactgg aattgactga aaaaggaatc aaccgtgagg
     2941 acttattcca gctgatttcc tattcttata ttttaaaagc tgagaaggct ggacttgttt
     3001 ttccgagcaa ggacaaggtc attgataatg agataggaaa cctagcaggt tatggagctt
     3061 tgttgaagaa atggtctatc caaattcctc agaagtcttc atcttatagt gcattttgta
     3121 aaatgatgga aaattcagaa gaaagtttta aagcgattat tgatgaagaa gtggggagaa
     3181 aataatttcc cctctttctt ttatttttaa cacaagaaaa atccgaacaa tatattttaa
     3241 ttcataaaat aatttgacaa aaactgtact ttggtttata ataaattaag gaaacgtcgg
     3301 aaaaggcgta gtgatgcgag agcataggta ggtcattaca aaagaaacga gacatcgata
     3361 tgttaaatga atttccaatt tttgattacg aagatattca attgattcca aataaatgtg
     3421 tcattaaaag ccgtgcagaa gcggatacaa gtgtcacttt aggaaatcac acctttaaac
     3481 tacctgttgt gccagcgaat atgcagacga ttttggatga aaatgtagca gagcaactgg
     3541 ctaaaggtgg ttacttctac attatgcacc gttttgatga ggcaggacgc attcctttta
     3601 ttaaacgaat gcacaatcaa gggctcattg cttccatatc tgtcggtgtt aaggactatg
     3661 agtatgattt cgttagacag ctcaagactg atgctcctga gtatatcact attgatattg
     3721 ctcatggtca tgcggatagc gtgatttcta tgattcaaca catcaagaaa gaattgccag
     3781 atacctttgt cattgctgga aatgtgggaa caccagaagc tgtgcgtgaa ttggaaaatg
     3841 ctggtgcgga tgctactaag gtcggaatcg gtcctggtaa ggtttgtatc accaaggtta
     3901 agactggttt tggtacaggt ggttggcagt tggctgctct acgctggtgt gccaaggctg
     3961 cgcgtaaacc gattatcgct gatggaggaa ttcgtactca cggtgatatt gccaagtcta
     4021 tccgcttcgg tgctagcatg atcatgattg gttccctctt tgcaggacat attgaaagtc
     4081 cagggaaaac gattgaagtc gatggtgaac agttcaaaga atattatggt tcagcctcac
     4141 aatatcaaaa aggtgcttac aaaaatgtgg aaggcaaacg tatcttgctt cctgctaaag
     4201 gtcatctgca agacacttta actgagatgg aacaagacct tcaaagtgct atttcttatg
     4261 caggtggacg tcaggttgct gaccttaaac acgttgatta tgtgatcgtg aaaaactcca
     4321 tctggaatgg ggatgcttcc cactaagacg gggtctttga ctaaaaaact tgttattaga
     4381 gcaaatttct gttataataa aacaagtttc cacccttagt gtaatggata tcacgtaaga
     4441 ttccggttct tgagatgggg gttcgattcc ctcagggtgg atgtaaacat tctaaaaagc
     4501 ctttaatagg gcttttttct ttacactact ctaaatttgc cactaaaact gagaatctct
     4561 gtctaaagaa ttcccaaatg ctttccagtc ccgttttaga gtgactcagg gggctttttt
     4621 tgatataata aaaaggactg ttatcagtta gaaagaggtt ggtatgaaag aattacaaac
     4681 tgtactaaag aaccattttg caatcgaatt tacagacaaa aagttactgg aaactgcctt
     4741 tactcatacg agttatgcca atgagcaccg cctcttaaaa atttcacata atgaacgctt
     4801 ggaattttta ggagacgctg ttctacagtt attgatttca gaatatctgt ataaaaaata
     4861 tcctaaaaag cctgaaggtg acctatcaaa actccgtgct atgattgtcc gtgaggagag
     4921 tttagctggt tttgcgcgtg attgccagtt tgaccagttt atcaagttgg gtaaagggga
     4981 agaaaaatct ggtggtcgca atcgtgacac cattcttggt gatgcctttg aagcctttct
     5041 tggtgccctt cttttggata aggatgtggc caaggtcaag gaatttatct atcaagtcat
     5101 gattcctaag gttgaagcag gcgagtttga gatgattaca gactataaaa cccatctcca
     5161 agagttgctt caggtcaatg gtgatgtggc tattcgttat caggtgattt ctgaaacagg
     5221 gcctgctcac gataaggtct ttgatgtaga agttcttgtt gaaggtaaga gcatcggtca
     5281 aggccaaggt cgttctaaga aattagcaga gcaggaagct gccaaaaatg ccgttgagaa
     5341 ggggctggat tcatgtattt aaaggaaatc gaaattcagg ggttcaagtc ttttgctgat
     5401 aagaccaagg tcgtttttga ccaaggtgtg acggcagttg ttggacccaa tggatctgga
     5461 aagtccaata ttacagaaag tctgcgttgg gctttggggg agtctagtgt caagagtctc
     5521 cgtgggggca agatgccgga tgtcatcttt gctggaacag aaagtcgcaa accgctcaat
     5581 tatgcttctg tagttgtgac tctggataat catgacggat ttatcaagga tgcaggtcaa
     5641 gaaatcaggg tggaacgcca tatctatcgt agtggagata gcgaatacaa gattgacggc
     5701 aagaaagtcc gtctgcgtga tattcatgac ctcttcttgg atactggatt gggacgagat
     5761 tccttctcta ttatttccca agggaaggtt gaggagattt ttaattccaa gcctgaggaa
     5821 cgacgagcta tttttgaaga agctgctgga gttttaaaat acaagactcg cagaaaagaa
     5881 accgagagta aactgcagca aactcaggat aatctggacc gcttagagga cattatctac
     5941 gagttggata atcaaatcaa gcctcttgag aagcaagctg agaatgcccg taagttttta
     6001 gacttggaag gacaacgtaa ggctatttat ttagacgttc tggttgctca aatcaaggaa
     6061 aataaggcag aactagagtc gacagaagaa gagttggctc aggttcaaga actcttgatg
     6121 agttattacc aaaagcgtga aaaattagaa gaagaaaatc aaactcttaa aaagcaacgc
     6181 caagatttac aggctgaaat ggccaaagac caaggcagtt tgatggactt gactagtctg
     6241 attagtgatt tagaaagaaa attagcccta tcgaaactgg agtccgagca agtggccctg
     6301 aatcaacagg aggcacaagc tcgtttggct gctttggagg ataagagaaa ttcactcagc
     6361 aaagaaaagt atgataaaga aagctcttta gctctgttag agggaaatct agtccaaaat
     6421 aatcaaaaac tcaatcgttt agaagctgaa ttgctggctt tctcagacga tcctgatcag
     6481 atgattgagc tcttacgtga acgctttgta gctcttttac aagaagaagc ggatgtctca
     6541 aaccagttga cccgtattga gaatgagttg gaaaatagtc gtcagctttc tcaaaaacaa
     6601 gcagatcaac tagaaaagct gaaagagcaa ttagctacag ctaaagagaa ggctagtcag
     6661 caaaaagacg agcttgaaac tgccaaggtg caggttcaga aattattggc tgactatcaa
     6721 gctattgcca aggagcaaga ggagcagaaa acttcctatc aagctcaaca aagtcaactc
     6781 tttgaccgtc tggatagtct caaaaacaag caggccagag ctcaaagttt ggaaaatatc
     6841 ctgagaaatc atagtaactt ttatgcaggt gttaagagtg ttctccaaga aaaagatcgc
     6901 ctaggtggga ttattggtgc agtcagtgag catctgacct ttgatgttta ttatcaaact
     6961 gccctagaga ttgccttagg ggcaagtagc cagcatatca tcgtagaaga tgaagagtcg
     7021 gcaaccaaag ctattgattt cctcaaacga aacagagtcg gtcgtgcaac ctttcttcct
     7081 ttgaccacta ttaaggcgcg tacgatttct agtcagaacc aagatgctat cgctgtaagc
     7141 ccaggtttcc ttgggatggc agatgagttg gtgacttttg atactagact ggaagccatt
     7201 ttcaagaact tgctagctac gacggctatc tttgataccg tagaacatgc gcgtgaagct
     7261 gctcgacaag ttcgttatca ggttcgtatg gtgacattgg atgggacaga attacgcacg
     7321 ggtggttcct atgcgggtgg tgccaatcgc caaaataaca gtattttcat caagccagaa
     7381 ctggagcaat tacaaaaaga aattgctgca gatgaagcaa gcttgggttc agaagaagcg
     7441 gctttgaaga ccttgcaaga ccagatggct gcattgacag aaagattaga agccatcaaa
     7501 tctcaaggag agcaggcacg tattcaggag caaggcttgt ccctcgctta tcagcaaact
     7561 agtcagcaag ttgaagaact ggaaactctt tggaaactcc aagaagagga aatagatcgt
     7621 ctttctgagg gagattggca agcggataag gaaaaatgtc aagagagcct tgctactatc
     7681 gccagtgaaa agcaaaatct ggaagctgag attgaagaaa ttaagtctaa taaaaacgcc
     7741 atccaagaac gctatcaaaa tttgcaggaa gaggtggcgc aagctcgctt gcttaagaca
     7801 gaactgcaag ggcaaaaacg ttatgaagta gctgatattg agcgtttagg caaggaattg
     7861 gacaatctta atatcgaaca agaagaaatt cagcgcatgc tccaagaaaa agttgacaat
     7921 cttgagaagg ttgatacaga attgctcagt caacaggcgg aagaatccaa aactcagaaa
     7981 acaaatctcc aacaaggttt gattcgcaag cagtttgagt tggatgatat agaaggtcaa
     8041 ctggatgata ttgccagtca cttggatcaa gctcgccagc agaatgagga gtggattcgc
     8101 aagcaaacac gtgctgaagc caagaaagaa aaggtcagcg agcgcttgcg ccatctacaa
     8161 aatcaattaa cagaccagta ccagattagc tatactgaag cactagaaaa ggcacatgaa
     8221 ttggaaaacc tcaatctggc agagcaagag gtgcaggatt tagagaaggc tattcgctca
     8281 ttgggacctg tcaacttgga agctattgac cagtacgaag aagttcacaa ccgtctggac
     8341 tttctaaata gtcagcgaga tgatattttg tcagcgaaaa atctgctcct tgaaaccatt
     8401 acagagatga atgatgaggt caaggaacgc tttaaatcaa cctttgaagc tattcgtgag
     8461 tcctttaaag tgaccttcaa gcagatgttt ggcggaggtc aggcagactt gatattgact
     8521 gagggcgacc ttttaacagc tggtgtggag atttctgttc aacctccagg taagaaaatc
     8581 cagtcgctta acctcatgag tggtggtgaa aaagctctat cggctcttgc cttgcttttc
     8641 tccattattc gtgtcaagac cattcctttt gtcatcttgg atgaggtgga agctgcgctg
     8701 gatgaagcca atgttaaacg ttttggggat tacctcaacc gctttgacaa ggacagccag
     8761 tttatcgttg taacccaccg taagggaacc atggcagcgg ctgattccat ctatggagtg
     8821 accatgcaag aatcgggtgt ttcaaagatt gtttcagtta agttaaaaga tttagaaagt
     8881 attgaaggat gacaattaaa ctagtagcaa cggatatgga cggaaccttc ctagatggga
     8941 atggacgctt tgatatggat cgtctcaagt ctctcttggc ttcctacaag gaaaaaggga
     9001 tttactttgc ggtggcttcg ggtcggggat ttctgtctct ggaaaaatta tttgctgatg
     9061 ttcgtgatga cattattttc atcgcggaaa atggcagttt ggtagagtat caaggtcagg
     9121 acttgtatga agcgactatg tctcgtgact tttatctggc aacttttgaa aagctgaaaa
     9181 cgtcacctta tgtagatatc aataaactgc tcttgacggg taagaagggt tcatatgttc
     9241 tagatacggt tgatgagacc tatttgaaag tgagtcagca ttataatgaa aatatccaaa
     9301 aagtagcgag tttggaagat atcacagatg acattttcaa atttacaacc aacttcacag
     9361 aagaaacgct agaagctggt gaagcttggg ttaatgaaca tgtccctggt gttaaggcca
     9421 tgacaactgg ctttgaatct attgatattg ttctggacta tgtcgataag ggtgtagcta
     9481 ttgttgaatt agctaaaaaa cttggtatca caatggatca ggtcatggct tttggagaca
     9541 atcttaatga cttacatatg atgcaggttg tgggacatcc tgtagctcct gaaaatgcac
     9601 gacctgaaat tttagaatta gcaaagactg tgattggtca ccataaggaa cggtcggtta
     9661 tagcttatat ggagggctta taatggcaga tataaaattg attgcattgg acttggacgg
     9721 gaccttgctg actactgata aaaggctgac ggatcgtacc aaggaaacct tgaaagctgc
     9781 gcgtgatcgt ggtatcaagg tcgtattgac aactggtcgt cccttaaaag ccatggattt
     9841 ctttctccat gagttaggga ctgacggtca ggaagatgag tataccatta cttttaatgg
     9901 tggattagtt cagaaaaata caggagaaat ccttgataaa acagtctttt catatgatga
     9961 tgtggcacgt ttgtatgaag aaacagagaa attatcactg cctcttgatg ccatctcaga
    10021 aggaacagtt tatcaaatcc aatcggacca agaaagtctt tatgccaaat tcaatccagc
    10081 tttgaccttt gttccagtgg actttgaaga cttatctagt caaatgacct acaacaaatg
    10141 cgtgactgcc tttgctcaag aacccttgga tgcagccatt cagaagattt ctccagaatt
    10201 gtttgaccaa tatgaaatct ttaaatcacg tgaaatgttg ctagaatggt caccaaagaa
    10261 tgttcataaa gcaacaggtt tggcaaaact aatcagccat cttggaatcg accaaagtca
    10321 agtgatggct tgtggtgacg aggccaatga cctctctatg attgaatggg caggtcttgg
    10381 tgttgctatg caaaacgctg ttcctgaagt aaaggcagcc gcaaatgtag tgacgccgat
    10441 gaccaacgat gaggaagctg tcgcctgggc tatcgaagaa tatgtgctaa aggagaacta
    10501 agatatggga ttgtttgacc gtctattcgg aaaaaaagaa gaacctaaaa tcgaagaagt
    10561 tgtaaaagaa gctctggaaa atcttgattt gtctgaagat gttgatccta ccttcacaga
    10621 agttgaggaa gtttctcagg aagaagcaga ggttgaaatt gttgaacaag ctgtgttcca
    10681 agaagaggaa atccaagaca cagttgaaga aagtctggat ttagagccag ttgtagaagt
    10741 ttctcaaaaa gaagtcgaag aatttccaca ctcagaagaa gggaatactg agtttctaga
    10801 gactatagaa gaaaataatt ctgaagttct tgaaccagaa aggcctcaag cagaagaaac
    10861 cgttcaggaa aaatatgacc gcagtcttaa gaaaactcgt acaggtttcg gtgcccgctt
    10921 gaatgccttc tttgctaact tccgctctgt tgacgaagaa tttttcgagg aactggaaga
    10981 actgctgatt atgagtgatg ttggtgtcca agtcgcttct aacttaacgg aggaactacg
    11041 ttacgaagcc aagcttgaaa atgccaagaa acctgatgca cttcgtcgtg tcatcattga
    11101 gaaattggtt gagctttatg aaaaggatgg tagctacgat gaaagcatcc acttccaaga
    11161 taacttgaca gttatgctct ttgttggtgt gaatggtgtt gggaaaacaa cttctatcgg
    11221 aaaactagcc caccgctaca aacgagctgg taagaaggtc atgctggttg cagcagatac
    11281 cttccgtgcg ggtgcagtag ctcagctagc tgaatggggc cgacgagtag atgttccagt
    11341 agtaactgga cctgaaaaag ctgatccagc cagcgtggtc tttgatggta tggaacgtgc
    11401 cgtggctgaa ggtatcgata ttctcatgat tgatactgct ggtcgtctgc aaaataagga
    11461 taaccttatg gctgagttgg aaaagattgg tcgtattatc aaacgtgttg tgccagaagc
    11521 accacatgaa accttcttgg cacttgatgc atcaacaggt caaaatgccc tagtacaggc
    11581 caaagaattt tcgaaaatca cacctttaac gggaattgtt ttgactaaga ttgatggaac
    11641 tgctcgagga ggtgtggttc tagccattcg tgaagaactc aatattcctg taaaattgat
    11701 tggttttggt gaaaaaatcg atgatattgg agagtttaac tcagaaaact ttatgaaagg
    11761 tctcttggaa ggtttaatct aatcagaagc aaaaatcctg caaggcataa acttgcagga
    11821 aattttttta ttctaagcga ccatcttgac gataggtgat atctggttgc caagtccatt
    11881 tggcaccgaa tttttcaagt aggtcaaagc tggcttgagg tcccatgctt ccagctttat
    11941 agtcatgaag tggggcacca ttttcagccc agagcttttc aatacggtca atcaacttcc
    12001 atgacgcaca aacttcatcc cagtggctaa agttagttga gttgttattt aggacatcat
    12061 aaatcaattt ttcgtatggt tctggagaag caccagttgc agtcgcatct gtacggtaat
    12121 caagtgagtt aggagccaag ttaaattctt ctcctacttg cttcccattt aggctaagag
    12181 agaagccttc tgttggttga atatagatgg tcaaaatatt tggagcaagt ggttctccaa
    12241 agatagaatc catttgttta aagacgatgt tgacatgagt tcctttttca gtcagtcgtt
    12301 tacctgtacg gaaaaagaaa ggaacaccac ggaatcgatc gctgtctaca aagaaggcac
    12361 cagatgtaaa ggtttcagtt gttgattctg gattcacatt tggctcgcta cgataagaga
    12421 tgtatttcat gccatcaatc ttaccagagc ggtattgccc acggataaag tgttctttga
    12481 gttcttcatc agttggatga tagaggtttt taaagacctt aatcttttca gcacgaatct
    12541 cgtcttttgt gaagcttgct ggtttgtcca tggcgaggag cgaaagaagt tgtagagtgt
    12601 ggttttggac catgtcacgg agggcaccgg attggtcata gtagccacca cgttcttcta
    12661 cacccaagcg ctccgcaaag gtaatttgaa cattgtcgat aaaatccttg ttccaaacgt
    12721 tttcaaaaat caagtttgca aagcgaactg caaagatgct ttggatcatt tccttaccaa
    12781 gataatggtc gatacggaaa atttgttctt cgtcaaatgt tgctaggagt tcgtcattca
    12841 acttgtttgc agttgcgtaa tctgtaccaa atggtttttc aacgatcaag cgctcaaaac
    12901 ctttgccatc gacaatgttt tcagatttga ggtgtttggc aatggttcca aagaactgag
    12961 gtgccataga caagaagaag agcttattgt gttcagcttg gtatttttca ttaagctcag
    13021 cttgtaattg acgcaaagca atataatgtt ccgaatcatt gacatcatgg ctttgatagt
    13081 agaagtggct agcaaattct tgggcttgct cggtactatc tgccaaatca aggatggact
    13141 cgacaactac agattcaaaa tattccttac tccaaggtct acgggcagtt ccaataacgg
    13201 caaagtgctt ggaaagattg ccggatttat atagtctaaa aagggaaggg tagagcttgc
    13261 gtttagccag gtctccactc gcaccgaaaa ttgtaacaat aaccttagat gacatctagc
    13321 tacctaattt ctatttttat tcctagtctt gctaggtatg aggaaacctc atttcataaa
    13381 cttaattcta acataatttt ccgaaaaatc aaaaattcca atacgaggta gaaaaactgc
    13441 aaggaaatcc ttacagtttg agtttagacg tttaagacct tatctaagaa ctctttcaga
    13501 cgagggtgtt gtgggttatc aaagatttgg tcaggtgttc cgtcttcaag gaactcgcca
    13561 tctgcagtaa agataacgcg gttggcaacc tgacgagcaa atcccatctc atgggttacg
    13621 ataatcatgg tcatgccttg ctcagccaat tccttcataa cgttaagtac gtctccaacc
    13681 atctcagggt caagggcaga agttggttca tcgaagagca tgatgtctgg attcattgct
    13741 aggccacgag ctatggccac acgttgtttt tgaccacctg ataggctatc tggattggca
    13801 ttagctttat ctgctagtcc aaccttttca agcaactcca ttcccaattt ctcagcttct
    13861 tccttagtca tcaacttgtg ctcaatagga gcaaaggtga tgttgtccaa tacagacata
    13921 tgagggaaga ggttgaagtg ttggaatacc atgccgatat tttcacggac gtggtcaaca
    13981 ttggttgttt tttcagttaa atcatagccg ttcacagtga tgtgaccgct agtgacttct
    14041 tctaaaagat tgaggctacg gaggaaagtt gacttaccag aacctgaagg accgatgata
    14101 caaacaacat ctccttcata gaacttagtc gtaattcctt ttaggacttc attttttcca
    14161 tagtgcttgt gtaaatcatt tacatcaatt tttaattttg ccattaacga atcctctttt
    14221 ctaagcgttt cgctagtcta gtcaaaagtg tgataattac aagatagaag atagcaagga
    14281 ttgcatacat cttgaaactt tggtagttac gagcaatgat aatcttacca gtttggaaga
    14341 gttcaaccaa accgatagca gatacgatag ttgtatcttt aagagcgata acgaattggt
    14401 tgacaaagtt tggcaacatc aatttagttg cttgtggcaa gataatctta cgcatggttt
    14461 ttccataaga gatacccaag cttcggctgg cttccatttg gccaactgga acggcctgaa
    14521 taccaccacg aacgatttca gcgatataag cagccgcatt gagtgagagg gcaatggttc
    14581 cagctacaaa gtcgttaatt gggctttgtt ggcctgtgat agactcgatg aagtttggaa
    14641 ttccccagaa gatgaaggct gcaagaatca tcaatggaat accacgaata acgtcaacga
    14701 aaatctcaga gatgacgcga agagatttgt atgggctaac gctaaacata ccgaagataa
    14761 ttccgatgac aatggcaata gcaaatgaga taagagctag agcaagagtg ataccaagac
    14821 cgctaaggag ttgtttgtag ttgttttgaa gcaagcccca gagcgttgtt tcgtcaacag
    14881 tacttgttga agcagttgaa gattcgctag ctaggtattt gtcaagaatc ttttggaatt
    14941 caccgtttgc tttaaggttt gcaagtccgt tgttgaacat ttcaatcagt tctggatttg
    15001 ctcctttttt aacggcaaag gctgtttcac cgattggagt tccagagatt ggagttttca
    15061 atttttgacc ttggctgata gaatatttga gaacaggttc atcatccata acggcatcaa
    15121 tggcaccagt gtttaaactg tcatacattg aagaaccatc agcaaaggtt ttgattttgt
    15181 agccgtattt gctttgattt tctgttagga aggtttgaga agcagttccg tttttaacac
    15241 cgactgtctt tccttttaga tcttcataag aagcaatatt acttgattct ttgacaccaa
    15301 gaatggtatt agcagtgtag tatgattctg agaagtcaaa agttgcctta cgagcatctg
    15361 tgacagacat accagcgatg ataccatcgg cttgaccagc ttggacagca ctgatagcag
    15421 catcaaaacc agggttggtg atttcaattt caaaaccttg gtctttagcg attgccttaa
    15481 tcaattccat atcaatacca gtgtattggt tgcttgaatt ttggaaaaca aaaggggcaa
    15541 aagaagaatc gctggcaatg atatatttag ccttaacagg aatagctttt aatcctgcga
    15601 gagtagttgt agttggcact gctgaagatg atgaagcagt ccatttcttg ataattttat
    15661 caagactacc atcttttttc atttcagaca aggcttggtt aaattcagta accaggtgct
    15721 cgtatttact tccttttttc acaccgaaag caaaacttcc tacagcttca ccatccattt
    15781 caatatggag gtcttgacct tggttaatgg catattcgat aacaggtttg tcatccatca
    15841 tggcatcgat ggcaccagca ctcaagctgt tgttcattaa atcaccagtg tcaaatgttt
    15901 taatagtaaa gccatattta tctttgattg tttcaaggaa acgttgagcg gcagttccgt
    15961 ttttaacacc aacggttttg ccagttaatt ggtcgtactt gctaattttg tgtgactttg
    16021 tagtagcaat gacaactttt gtatcatagt aagtatcaga catggtgaag actttttcac
    16081 gttctttagt ctttgtcatc cctgccatga tagcgtcggc ttgcccagct tgaaccgcat
    16141 tgactgctgc gtcaaatcca ggataggaca tctgaatgtt ccagccttta atctcagcga
    16201 ctttgttaat aatgtcaaca tcaattcctt tataagtttg atctgaatct ttaaactcaa
    16261 aaggtgcata ggcggtatca gaaacaatct taatcgtttc tgctttggca atacctaatg
    16321 agaaaattgg gaataaaatt agcaaaaatg ctagaaattt tttcttcatt tttagtctcc
    16381 ttttccgaat attctctcat tatatcagaa aaagtaaaaa aaggcaaatt gttatatttt
    16441 tcgtgtcaga aaatataaca ttgttttttc cttttctttc tt
//
LOCUS       DAVKZB010000035        16011 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKZB010000035 DAVKZB010000000
VERSION     DAVKZB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.035"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            136..684
                     /locus_tag="VXA80_001007"
     CDS             136..684
                     /locus_tag="VXA80_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV0353520.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            794..1693
                     /locus_tag="VXA80_001008"
     CDS             794..1693
                     /locus_tag="VXA80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV0353521.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1718..2992)
                     /gene="serS"
                     /locus_tag="VXA80_001009"
     CDS             complement(1718..2992)
                     /gene="serS"
                     /locus_tag="VXA80_001009"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV0353522.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3206..3577)
                     /locus_tag="VXA80_001010"
     CDS             complement(3206..3577)
                     /locus_tag="VXA80_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV0353523.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3580..4944)
                     /locus_tag="VXA80_001011"
     CDS             complement(3580..4944)
                     /locus_tag="VXA80_001011"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV0353524.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5313..6098
                     /locus_tag="VXA80_001012"
     CDS             5313..6098
                     /locus_tag="VXA80_001012"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV0353525.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6192..6626
                     /locus_tag="VXA80_001013"
     CDS             6192..6626
                     /locus_tag="VXA80_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0353526.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6626..7600
                     /locus_tag="VXA80_001014"
     CDS             6626..7600
                     /locus_tag="VXA80_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV0353527.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7660..7884
                     /locus_tag="VXA80_001015"
     CDS             7660..7884
                     /locus_tag="VXA80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0353528.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            8003..8977
                     /gene="fabK"
                     /locus_tag="VXA80_001016"
     CDS             8003..8977
                     /gene="fabK"
                     /locus_tag="VXA80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV0353529.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8970..9890
                     /gene="fabD"
                     /locus_tag="VXA80_001017"
     CDS             8970..9890
                     /gene="fabD"
                     /locus_tag="VXA80_001017"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV0353530.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVIPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9924..10655
                     /gene="fabG"
                     /locus_tag="VXA80_001018"
     CDS             9924..10655
                     /gene="fabG"
                     /locus_tag="VXA80_001018"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV0353531.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10677..11912
                     /gene="fabF"
                     /locus_tag="VXA80_001019"
     CDS             10677..11912
                     /gene="fabF"
                     /locus_tag="VXA80_001019"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV0353532.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11915..12400
                     /locus_tag="VXA80_001020"
     CDS             11915..12400
                     /locus_tag="VXA80_001020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV0353533.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12397..12819
                     /gene="fabZ"
                     /locus_tag="VXA80_001021"
     CDS             12397..12819
                     /gene="fabZ"
                     /locus_tag="VXA80_001021"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV0353534.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12831..14198
                     /gene="accC"
                     /locus_tag="VXA80_001022"
     CDS             12831..14198
                     /gene="accC"
                     /locus_tag="VXA80_001022"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV0353535.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14235..15101
                     /locus_tag="VXA80_001023"
     CDS             14235..15101
                     /locus_tag="VXA80_001023"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV0353536.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15098..15865
                     /locus_tag="VXA80_001024"
     CDS             15098..15865
                     /locus_tag="VXA80_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV0353537.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
BASE COUNT         4584 a         3044 c         3750 g         4633 t
ORIGIN      
        1 tggaagtgtg ataaactaat ataacaacga aaatcttatg agagaataaa gctttatctt
       61 agataaactt aggaatcagg attttataag attttccaat aatattagtg tcaataataa
      121 gaaaagaggt atcttatgac aacatttact atccatacag tagagtcagc accagcagaa
      181 gtgaaagaaa ttcttgaaac agtagaaaaa gacaacaatg gctatattcc caacctaatc
      241 ggtctcttgg ccaatgcccc gactgtttta gaagcctacc aaattgtctc atctatccac
      301 cgtcgcaaca gcctgacacc cgttgagcgt gaagtggtgc aaatcacggc agccgtgacc
      361 aatggttgtg ccttctgtgt cgcaggtcac acagcctttt ccatcaaaca aatccagatg
      421 aatgatgact tgattcaagc tcttcgcaat cgtactccaa ttgaaacaga tcctaaattg
      481 gataccctag ctaagtttac cttggcagtt atcaatacca agggtcgtgt aggagatgaa
      541 gccttgtctg agtttttaga agctggctac actcaacaaa atgccttgga tgtggttttt
      601 ggtgtcagcc tagcaatcct ctgtaactat gccaacaact tagctaatac accaattaat
      661 ccagaattgc aaccttatgc ctaattcata tctgagtaaa atgaagtctg aaataagcgg
      721 acttcgtttt tttaaatcct catttaagcg cttttatgtt atactgaaac taacatgatt
      781 attggaggtt aggatgaaaa aactcccctt agtattttca ggttgtttgc taggtttggc
      841 aggagctgga aatcttattt tagatacgtt gccggttcta tcccatcttt ttagtctgac
      901 tggtttggtt ttatggattt actttctaat tctgcatctc tttaattgga aagaaaccaa
      961 gcaagaattg accaagcccc ctcttttgtc aggaatggca acctttccta tggctgggat
     1021 gattttatcg acctatgtct ttcgcgtatt ctcttatctt cctttggtag cacaagggat
     1081 ttggtggttt tcatttctct tggatttgac cttgattgct ggttttacca tcaagtttgc
     1141 ttgtccaggg cggagggttc atgccactcc aagttggacg gttctctatg tggggatagc
     1201 agtggctgcc ttgacctatc ctctggtagg tattatcgaa attgcctatg cgaccttgag
     1261 ttttggtttt ctcttgacct tctatctcta tccccttatt tatagcgatt taaagaaaca
     1321 tccactccca ctagccttgc ttggacaaga aggaatctac tgtgctcctt tctctctact
     1381 cttggcttct ctagttcgag taggaggaac cagcctgccg acttgggtct tgattgtcat
     1441 gattttggct tctcaatcct tctttttctt tgttttaact cgtctgccca acattttaaa
     1501 acaaggtttt caaccagcct tctcagccct caccttccca accattatca cagcgacctc
     1561 gctcaagatg gctcagggaa ttttgaaact tccatttctg gattacctgg tattggctga
     1621 aaccattata tgcctaacta ttttattctt tgtactgggt gcttatctga tttggttacg
     1681 aaaaaaggtc tagctagaaa tagctaaacc ttatttttta tggtttgatg acttcagctc
     1741 cacccatgta tggacgaagt gcttctggga tggtcacaga accatcttca ttttggtaat
     1801 tttcaagaat tgcagccact gtacgtccaa ctgcaagtcc agaaccgttc aaggtatgaa
     1861 ggagtttcac cttgccatct gcttcatcac ggtaacggat ttgggcacga cgggcttgga
     1921 aatcttctgt gtttgaacag cttgagattt cacggtaatt gttttgtgct ggaatccaca
     1981 cttccaagtc gtaagtcttc gcagctgaga agcccatatc tccagtagag agagcaacga
     2041 cacggtatgg aaggttgagt ttttgaagaa tgttttcagc gttggctgtc attttttcca
     2101 attcttcgta agattcttct ggtttggcaa atttgaccat ttcaaccttg tggaattggt
     2161 gcaaacggat caagccacgc gtatcacgac cggcagaacc agcctcagaa cggaatgacg
     2221 gactcatggc agtgaagtag attggaagat ctttgccgtc taagatttca tcacggtagt
     2281 agtttgtcag aggaacttca gcagttggaa tcaagacaaa gttggtatcg ctgagttcaa
     2341 aagtatcttc cttaaatttt ggatactgac cagtaccaaa catagaatca tggttgacta
     2401 tgtaaggtgt gatgacttca gtatagcctt cttttccatg ttcatccaac ataaagttgt
     2461 agatagcacg ttccaaacga gcaccgaggc ctttatagaa gaggaagcga gcgcctgtta
     2521 ccttaccacc gcgttcccag tcaaggatac caaggtcttc accgagatcc cagtgagctt
     2581 taggttcgaa gtcaaactcg cgtggagtac cccaacggcg aacttccaca ttgtcgtctt
     2641 cgtcagtccc aacaggaacg ctgtcagctg ggatatttgg aagagtcgtt gtaaattctg
     2701 tcaatttagc atcgatttct gccaattcag catccaaggc tttaacctca gcagatagat
     2761 tttgcatggc agcaatcttg tcatctgtat tttccttgtt gcgcttagct tgggcaatct
     2821 cagcagaaac tgtgttacgt tctgctttga gagtttcaac cttgaccaag atgttacgac
     2881 gtttagcatc gatttctttc atttcattca agacagcagc atctacacca cgtgtagcta
     2941 atttttctgc gacagcttca aaatctgtac gaatacgttt gatatctaac ataagaactc
     3001 ctttatgaaa aaagcacgcc tgacaaaacg ttggagtggc agggccacgg ttccatccaa
     3061 cttcacaggt gtgcacttga ttgtgtatgt aattgttact aacggtagaa tttcacctat
     3121 cccccctatc tgctcgcagt acccgcagac tttctgaaag aagaagataa cctacttatc
     3181 cgttgctatg attatactaa agtttctact tttttgcaaa tagattttta aatttttggc
     3241 taattgtctg aatcagggtc ggaagtttga cgaccttgtc attgcctagt ttttcgcgtg
     3301 caattttgag aatggcacct gagtcttttg aagcaaagag gaattttcct ttgtctgtaa
     3361 agacttcgaa gtggcggctg attttgcgtc cagtgacatt ggctccaatc tgattgatat
     3421 ggctccaagg aatctggata aattgttcga cattgacatc tgggtaaaat tccaaagcct
     3481 gatctccgac aaggaatttc ccaactttcc cagcgataga gaggtaggaa gtgcctgtcg
     3541 tactgaggag tactgttttg ttaagtgatt gggccatgct tagtcttcct tactttctcc
     3601 aaaaaaggca ttgtagaggg ctttaattgc tgctttctct tggtctttat tgacaacaaa
     3661 cataatagaa acttcactag aaccttgaga catcatctgg atgttgattt tgttttcaga
     3721 tagagcgcgt gtcgcagtag cagtcactcc gatgtggctc ttcatttttt caccaacaat
     3781 cataatgata gaaaggtcgt gttcgatttc tgcatgatct actttagcct tttgaaccaa
     3841 ctgacgcagg atttcttctt ccttgatggg agttagttgg cgagaacgga gaatgataga
     3901 aagatcgtcg atacctgttg gcatatgttc ccaaccgatg ttaagttctt ccaggatttg
     3961 cagaaccttg cggccaaatc caacctcacg gttcatgagg tatttcgaca tgttaatgct
     4021 gacaaagcct gagtcaccag caattcccac aactggaaat tcatcattac tgtgttttag
     4081 aacgatacga gtacctggat ggtcagggtt gttggtattc ttgataacca gaggaatttt
     4141 tccacggtag gcaggaagaa gagcctcgtc atgaaggact gagaagcctg cataggccaa
     4201 ctcgcgcatt tcacggtagg tcaactcagg aatcgagtgt ggttggtgga taataccagg
     4261 gtgggctgca aagataccat caacgtccgt aaagttttca tagaggtcgg ctttgacacc
     4321 agcagcaatg atagaacctg taatatcaga acctccacgt gagaaagtac agatttgatt
     4381 ttccttagtg acaccaaaga aaccaggaat gacaaggact tcattggtgt ttgtcaattc
     4441 ttcaatcttg tcataacttg atggaatgat gcgagcgtga ccaggttcac ttgtgaccac
     4501 aatcccagct tctctagggt gcatatagcg tgcatcgata ccattttggt taaagtaggc
     4561 agcaatcaat ttggcattgt tattttcacc ggctgctagg aaagtatcgt agagaaattc
     4621 attttcttca ataggaagag tggccaaggc gtgaatgctt ttagaaattt tttctagcac
     4681 agctggtttt agtcccaatt cactaaccat agcagcatag cggtcgataa tccagctttg
     4741 gctcttgcta atatcgttac ccgcaacata gtcgcggtag tatttaatca gggcatccgt
     4801 aaccttagta tcttcagcat tgcgtttacc aggcgcagaa acgactacaa aacgacgctc
     4861 tgaatcgctt ttgacgatgt ttaaaacttt ttctaattga ctagcagagg caagagaact
     4921 acctccaaat ttaacaactt tcataagaac tcctaaagta agtattttat acgattatag
     4981 cagaaagaaa ggcttttttc aatgaagaaa ataagatgct ttctagccta aagcgagcct
     5041 ttcttatcgg ctgagacact ttcagtcgat tttttcttgc tttctgctcc taaaaaagat
     5101 atactttgac agtgaaataa tttaacactc aatgaaatca aagagcaaac taggaagcta
     5161 gccgcagact gctcaaagca ctgctttgag gttgtaagta agactgacga agtcagctca
     5221 aaatactgtt ttgaggttgc agatggaagc tgacgcggtt tgaagagatt ttcgaagagt
     5281 ataaactgct tataaaataa aaaggagccc tgatggaaca cattatttat cagcttgaag
     5341 aggacttggc aatccttacc ttgaaccgtc ctgaggtcgc aaatggtttt catattccca
     5401 tgtgtgagga gattttagaa gctctgactt tggcagaaga aaatccagct gtgcatttta
     5461 tcttaatcaa tgccaatgga aaggtcttct cagttggggg agatttggta gagatgaagc
     5521 gggcagtgga tgaggatgat attccatcat tgacaaaaat cgcagagttg gtcaatacga
     5581 tttcttataa aatcaagcaa atagccaaac ctgttttaat ggaagttgac ggtgctgttg
     5641 caggtgccgc agcgaatatg gctgttgcgg cagatttctg tttggcgacg gataaggcta
     5701 agtttatcca agcctttgtt ggtgttggtt tggctccaga tgcaggggga attcatctct
     5761 tgagccgcag tatcggtgtg acgcgtgctg ctcaattagc tatgacagga gaggctttaa
     5821 cggcagaaaa agccctagaa tgggggttgg tttaccgcgt ttctgaagct gaaaaacttg
     5881 aaaagacgag agaacagctt cttaaaaaat taagacgtgc ttcaagtaat tcctatgctg
     5941 ccattaagaa gttggtttgg gagagccaat ttaaagactg gcagggttac gctactttag
     6001 aactgaacct acagaaatcc ttagctcaaa cagaggattt caaagaagga gtgcgagctc
     6061 attcggagag aagaagacct aaatttacag gaaaataaaa aatacttgca tcattctttg
     6121 aattttgata taattctttc tgtcaaatgt tttgattgta aaagtttttt gaagaaggag
     6181 ggatatgaaa aatggactac caacgaatca atgaatattt aacatctata tttaacaatg
     6241 tccttgtaat tgaggaagtg aacttgagag gtagtcgttt taaggatatc tccatcaaag
     6301 aaatgcatac gattgatgtc atcggtaagg ctccagatgt gactccaagt caagtgtcaa
     6361 aagagttgat ggtaactctt ggaactgtta cgacaagttt gaacaattta gagcgtaagg
     6421 gctacattga gcgagttcgg tcagaacagg atcgtcgtgt ggtgcatctg catttgacaa
     6481 agaagggtcg cttgattcat agactgcata aacgcttcca caaggccatg gtagaaaaaa
     6541 ttattgatgg catgagcgag gaagaaattg ctgtcatggg taaaggcttg actaatcttt
     6601 accaattttt ggaggatttg aaataatggc ttttgcaaaa ataagtcagg ttgctcatta
     6661 tgtgccagag caagtggtta caaatcacga cttggctcag attatggata ccaatgatga
     6721 gtggatttca agtcgaacgg gaatacgaca aaggcatatt tcaagaacag aatctaccag
     6781 tgatttggct acagaggttg ctaagaaact gatggcaaaa gctggaataa caggagaaga
     6841 actggatttt atcatcctag ctaccattac tccagattcg atgatgccct ctacagctgc
     6901 tcgtgttcaa gctaatattg gtgctaataa agcctttgct tttgacttaa ccgcggcttg
     6961 cagtggattt gtatttgctc tttcaactgc tgaaaagttt atcgcttctg gtcgctttca
     7021 aaaaggcttg gtgattggta gtgaaaccct ctctaaggca gtcgattggt cggatcgatc
     7081 aacagctgtg ttgtttggag atggtgctgg tggtgtcttg ttagaagcta gcgagcaaga
     7141 gcatttctta gctgagagtc tcaatagcga tggaagtcgc agcgagtgtt taacttatgg
     7201 gcattcaggt ttgcattctc cattttcaga tcaagaaagt gcagattcgt ttttgaagat
     7261 ggatggacgc acagtttttg attttgccat tcgagatgta gccaagtcta tcaagcagac
     7321 tattgatgaa tctcctatag aggtgacaga cttggattat ctgctacttc atcaagccaa
     7381 tgaccgtatt ttggataaga tggctagaaa aattggtgtt gaccgagcca aacttccagc
     7441 caatatgatg gaatatggca ataccagtgc agccagtatc ccgattttac tttcagagtg
     7501 tgtagaacaa ggtctcatcc ctttagatgg tagccagact gttcttctat caggcttcgg
     7561 tggaggcttg acctggggca cgctcattct tacaatttag gtaatcatgt ggtgaacaca
     7621 ttgttataaa aaaactattt attttaaagg agtcctatca tggcagtatt tgaaaaagta
     7681 caagaaatta tcgttgaaga acttggaaaa gacgcatcag aagtaacact tgaatcaact
     7741 tttgatgatt tggacgcaga ttcattggac ttgttccaag taatctcaga aatcgaagat
     7801 gcttttgata tccaaatcga agcagaaaat gacttgaaaa cagttggtga cttggttgct
     7861 tacgttgaag agcaagcaaa ataagcagaa tattagtaga aggagtaggg agaaacccac
     7921 tccctcttgt ttaggtaata gtttgactgt caaattatgg tgaaatcgaa ctattacgta
     7981 agcaaggaag gttggaggca ctatgaaaac gcgtattaca gaattattga agattgacta
     8041 tcctattttc caaggaggga tggcctgggt tgctgatggt gatttggcag gggctgtttc
     8101 caaggctgga ggattaggaa ttatcggtgg gggaaatgcc ccgaaagaag ttgtcaaggc
     8161 caatattgat aaaatcaaat cattgactga taaacccttt ggggtcaaca tcatgctctt
     8221 atctcccttt gtggaagata tcgtggatct cgttattgaa gaaggtgtta aagttgtcac
     8281 aacaggagca ggaaatccaa gcaagtatat ggaacgtttc catgaagctg ggataatcgt
     8341 tattcctgtc gttcctagtg tcgctttagc taaacgcatg gaaaaaatcg gtgcagacgc
     8401 tgttattgca gaaggaatgg aagctggggg gcatatcggt aaattaacaa ccatgacctt
     8461 ggtgcgacag gtagccacag ctgtatctat tcctgttatt gctgcaggag gaattgcgga
     8521 tggtgaaggt gctgcggctg gctttatgct aggtgcagag gctgtacagg tggggacacg
     8581 gtttgtagtt gcaaaagagt cgaatgccca tccaaactac aaggagaaaa ttttaaaagc
     8641 aagggatatt gacactacga tttcagctca gcactttggt catgctgttc gtgctattaa
     8701 aaatcagttg actagagatt ttgaactggc tgaaaaagat gcctttaagc aggaagatcc
     8761 tgatttagaa atctttgaac aaatgggagc cggtgcccta gccaaagcag ttgttcacgg
     8821 tgatgtggag ggtggctctg tcatggcagg tcaaatcgca gggcttgttt ctaaagaaga
     8881 aacagctgaa gaaatcctaa aagatttgta ttacggagcc gctaagaaaa ttcaagaaga
     8941 agcctctcgc tggacaggag ttgtaagaaa tgactaaaac agccttttta tttgctggtc
     9001 aaggtgccca gtatctaggg atgggacggg atttctatga tcagtatccg attgttaaag
     9061 aaacgattga tcgagcgagt caggtgctag gttatgattt gcgttatctc atcgatacgg
     9121 aagaagacaa actcaatcag acccgctata cgcaaccagc cattctagcg acttcggttg
     9181 ctatctaccg tttattgcaa gaaaagggct atcagcctga tatggttgct ggtttgtctc
     9241 ttggagaata ctctgccttg gtggcaagcg gcgccttgga ttttgaagat gcggttgcct
     9301 tggtagctaa gcgtggagcc tatatggaag aagcggctcc tgctgactct ggcaagatgg
     9361 tagcagttct caatacgcca gtagaggtca ttgaagaagc ctgtcaaaaa gcttctgaac
     9421 ttggagtggt tattccagcc aactataaca cacctgcaca aatcgtcatt gctggagaag
     9481 tggttgcagt tgatcgagcg gttgaacttt tgcaagaagc aggtgccaaa cgcttgattc
     9541 ctcttaaggt gtcaggtccc tttcacaccg ctctccttga gcctgctagc cagaaactag
     9601 ctgaaactct agctcaggta agtttttcag attttacttg tcccctagtc ggcaatacag
     9661 aagctgctgt gatgcaaaaa gaggacattg ctcagctctt gacgcgtcag gtcaaggaac
     9721 ccgttcgttt ctatgaaagt attggggtca tgcaagaagc aggcataagc aactttatcg
     9781 agattggacc ggggaaagtc ttgtcaggtt ttgttaaaaa aattgatcaa actgctcact
     9841 tagctcatgt ggaagatcaa gcgagtttag tagcactttt agaaaaatag actaaaataa
     9901 gtagaagttt tgaaaggaaa aaaatgaaac tagaacataa aaatatcttt attacaggtt
     9961 cgagtcgtgg aattggtctt gccatcgccc acaagtttgc tcaagcagga gccaacattg
    10021 tcttaaacag tcgtggggca atctcagaag aattgctcgc tgagttttca aactatggta
    10081 tcaaggtggt tcccatttca ggagatgtat cagattttgc agacgctaag cgtatgattg
    10141 atcaagctat tgcagaactg ggttcagtag atgttttggt caacaatgca gggattaccc
    10201 aagatactct tatgctcaag atgacagaag cagattttga aaaagtgctc aaggtcaatc
    10261 tgactggtgc ctttaatatg acacaatcag tcttgaaacc gatgatgaaa gccagagaag
    10321 gtgctatcat taatatgtct agtgttgttg gtttgatggg gaatattggt caagctaact
    10381 atgctgcttc taaggctggc ttgattggct ttaccaagtc tgtggcacgc gaggtcgcta
    10441 gtcggaatat acgagtcaat gtgattgctc caggaatgat tgagtctgat atgacagcta
    10501 tcttatcaga taagattaag gaagctacac tagctcagat tccgatgaaa gaatttgggc
    10561 aggcagagca ggttgcagat ttgacagtat ttttagcagg ccaagattat ctaactggtc
    10621 aagtgattgc cattgatggt ggcttaagta tgtagcgaaa gctagaggtg aaaagaatga
    10681 aactaaatcg agtagtggta acaggttatg gagtaacatc tccaattgga aatacaccag
    10741 aagaattttg gaatagttta gcaactggga aaatcggcat tggtggcatt acaaaatttg
    10801 atcatagtga ctttgatgtg cataatgcgg cagaaatcca agattttccg ttcgataaat
    10861 actttgtaaa aaaagatacc aaccgttttg ataactattc tttatatgcc ttgtatgcag
    10921 cccaagaggc tgtaaaccat gccaatcttg atgtagaggc tcttaatagg gatcgttttg
    10981 gtgttatcgt tgcatctggt attggtggaa tcaaggaaat tgaagatcag gtacttcgcc
    11041 ttcatgaaaa aggacccaaa cgtgtcaaac caatgactct tccaaaagct ttaccaaata
    11101 tggcttctgg gaatgtagcc atgcgttttg gtgcaaacgg tgtttgtaaa tctatcaata
    11161 ctgcctgctc ttcatcaaat gatgcgattg gggatgcctt ccgctccatt aagtttggtt
    11221 tccaagatgt gatgttggtg ggaggaacag aagcttctat cacacctttt gccatcgctg
    11281 gtttccaagc cttaacagct ctctctacta cagaggatcc aactcgtgct tcgatcccat
    11341 ttgataagga tcgcaatggg tttgttatgg gtgaaggttc agggatgttg gttctagaaa
    11401 gtcttgaaca cgctgaaaaa cgtggagcta ctatcctggc tgaagtggtt ggttacggaa
    11461 atacttgtga tgcctaccac atgacttctc cacatccaga aggtcaggga gctatcaagg
    11521 ccatcaaact agccttggaa gaagctgaga tttctccaga gcaagtagcc tatgtcaatg
    11581 ctcacggaac gtcaactcct gcaaatgaaa aaggagaaag tggtgctatc gtagctgttc
    11641 ttggtaagga agtacctgta tcatcaacca agtcttttac aggacatttg ctgggggctg
    11701 cgggtgcagt agaagctatc gtcaccatcg aagctatgcg tcataacttt gtaccaatga
    11761 cagctgggac aagtgaagta tcagattata tcgaagctaa tgtcgtttat ggacaaggct
    11821 tggagaaaga aattccatac gctatttcaa atacttttgg ttttggaggc cacaatgcag
    11881 ttcttgcttt caaacgttgg gagaatagat aagtatgaat ttaaacgata ttaaagactt
    11941 gatgactcaa tttgaccagt caagtttgag agaattttct tataaaaatg ggacggatga
    12001 gttgcagttt agcaagaatg aagcaagacc tgtgcctgaa gttgcaactc aagtcgctcc
    12061 agcacccgtt ctagcaacac cgagtccagt agctcctaca tctgctccag cagagactgt
    12121 agcagaagaa gttccagctc cagctgaagc aagtgtggct actgagggaa atcttgtaga
    12181 gagtccactt gttggagtgg tttacttggc tgctggacca gataaacctg ccttcgttac
    12241 agttggtgat agtgtcaaaa aaggtcaaac attggtaatt atcgaagcca tgaaagtcat
    12301 gaatgaaatc ccagctccta aggatggtgt ggtaacggaa attctcgtct ctaacgaaga
    12361 aatggttgag tttggtaaag gattggtacg tatcaaatga tcgatattca aggaatcaaa
    12421 gaagctcttc cccaccgtta tcctatgctt ctagtggacc gtgtcttgga agtgagcgag
    12481 gataccattg ttgctatcaa aaatgtgacc attaacgaac ctttctttaa cggccacttt
    12541 cctcaatacc cagttatgcc aggtgttctg attatggaag ccttggcgca aactgctggt
    12601 gtgttggagt tatcaaaacc tgaaaataaa ggaaaactgg tcttttacgc tggtatggac
    12661 aaggttaagt tcaagaagca agttgtacca ggcgaccaat tggttatgac agcgactttt
    12721 gtaaaacgtc gtggcaccat agctgtggtt gaagcaaagg ctgaagtgga tggcaagctt
    12781 gcagccagtg gtatccttac ttttgcaatt gggaactaag gaggttctcc atgtttcgaa
    12841 aaattttaat tgccaatcgt ggtgaaattg cggttcgtat tatccgtgcg gcacgtgaat
    12901 tggggattgc gacggtagcg gtttattcaa ctgctgataa ggaagctctt catacgcttt
    12961 tggcagatga agcagtttgt attggtcctg gcaaggcaac agagtcttat ctcaatatta
    13021 atgcagttct atcagctgca gtcttgactg aggcagaagc tattcaccct ggttttggat
    13081 ttctcagtga aaattccaaa tttgcgacca tgtgtgaaga agtaggtatc aagtttatcg
    13141 gtccatctgg tcatgttatg gatatgatgg gggataagat caatgcgcgt gctcagatga
    13201 ttaaagcagg tgtgcctgtt ataccaggtt cagatggaga agtgcataac tctgaagaag
    13261 ctttgattgt tgctgaaaaa attggctatc ctgttatgct caaggcttca gcaggcggag
    13321 gtggtaaagg gattcgtaag gttgaaaaac cagatgacct cgtttctgcc tttgaaactg
    13381 cctctagtga ggccaaggcc aattatggca atggtgccat gtacatagaa cgggttatct
    13441 atccagctcg gcacattgag gttcaaatcc taggtgatga gcatggacat gtgattcact
    13501 tgggtgagcg ggattgttct cttcaaagaa ataaccaaaa ggttttggaa gaaagtccct
    13561 cgattgcaat cggaaaaacg ctgcgtcatg aaataggtgc tgctgctgtt cgagcggcag
    13621 agtttgttgg ctatgagaat gcaggaacca ttgaatttct tcttgatgaa gcaagtagca
    13681 atttctattt catggagatg aatactcgtg ttcaggtaga acatccagta acagagtttg
    13741 tttcaggtgt tgatatcgtt aaggaacaga tttgcattgc ggcaggtcag cctttgtctg
    13801 ttaagcaaga agatattgtc ctacgcggtc atgccatcga gtgtcgtatc aatgcagaaa
    13861 acccagcctt taactttgct ccaagtccag gtaagattac taatctctat ctgccaagtg
    13921 gtggagttgg cttgcgcgtg gattcagcag tttatccagg ttataccatt ccgccttatt
    13981 atgatagtat gattgccaaa atcatagtac acggcgaaaa tcgttttgac gccttgatga
    14041 aaatgcaacg tgccctctat gaattagaga ttgaaggagt gcagaccaat gcagatttcc
    14101 agcttgacct catttcagat cgcaatgtca ttgctgggga ttatgatact tccttcttga
    14161 tggaaacctt cttacctaaa tatcaagaaa aagaataaaa tgactttaag agtttaaacc
    14221 gaaaagggga atcaatggct ctatttagta aaaaagataa gtatattcga atcaatccca
    14281 atcgttcggt tagggaaaaa cctcaagcta agccagaggt tccagatgaa ttattttccc
    14341 agtgtccagg ctgtaagcat accatctatc agaaggatct gggaagtgaa cgtatctgtc
    14401 cgcactgtag ctataccttt cgtatttctg cccaagaacg cttggctttg acgattgata
    14461 tgggaacctt caaagaattg tttacaggga ttgaaagcaa ggatcccttg catttccctg
    14521 gttaccaaaa gaaactggca tctatgcgtg aaaaaacagg tctgcatgaa gccgttgtga
    14581 caggaactgc tcttattaaa ggtcagactg tggctcttgg gattatggat tctaacttta
    14641 tcatggcttc tatgggtacg gttgtaggtg aaaaaatcac tcgtttgttt gagtatgcga
    14701 ctgtcgaaaa attgccagtt gtcctattca cagcctctgg tggagcccgt atgcaggaag
    14761 gaatcatgag tctcatgcag atggctaaga tctctgcggc ggttaaacgc cattcaaatg
    14821 ctggtctctt ttacctgacc attttgacag atccaacgac tggtggtgtg acagcatctt
    14881 tcgctatgga aggcgatatc attctggctg aaccacagag cttggttggt tttgctgggc
    14941 gtcgtgtgat tgaaaatacg gttcgtgaaa gcttgcctga ggatttccaa aaggcagaat
    15001 tcctattaga acatggcttt gtggatgcta ttgtcaaaag aagagactta ccagatacga
    15061 ttgctagcct agtcagattg catggaggga gtcctagatg aatattgcaa aaatagtcag
    15121 agaagcgcgt gagcagagtc gcttgacaac cttggacttt gcgacaggca tttttgatga
    15181 atttatccaa ttacatggtg accgttcttt tcgtgatgat ggtgcagttg ttggtggtat
    15241 tggttggctt ggagaccaag ctgtaacagt ggttggtatc caaaaaggca agagtttgca
    15301 agacaacctc aaacgaaatt ttggccaacc acatccagaa ggctaccgaa aggcactgcg
    15361 gttgatgaaa caggctgaga aatttggccg tccagttgtg acctttatca atacagcagg
    15421 tgcttatcct ggtgtcggag cggaagaacg tggtcaaggg gaagctatcg ctcgcaatct
    15481 catggaaatg agtgacctga aagttcctat tatcgccatt attatcggtg aaggtggttc
    15541 aggcggggct ctggctctag ctgtcgcgga ccgtgtctgg atgctggaaa attctatcta
    15601 tgccattctc agtccagaag gctttgcttc cattttatgg aaggacggta ctcgcgccat
    15661 ggaagcagca gaactgatga aaatcacttc gcatgaactg ttagaaatgg acgtggtgga
    15721 taaggtgatt tctgaagtag gactttctag taaagaactg attaagagtg tcaaaaaaga
    15781 actccaaacg gagctggcta gactttcaca aaaaccgcta gaagagttgc tggaagaacg
    15841 ctatcaacga tttagaaaat actaagaatg actaaatgag actagaaagg tctcattttt
    15901 tatggaaaaa tgcaattcag gtcgaaaaat tgccagttga ggagatagac tataaaatag
    15961 acaaaaaaag aaatatacta taaatgtgag cctacaacaa tctattttaa g
//
LOCUS       DAVKZB010000036        15958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKZB010000036 DAVKZB010000000
VERSION     DAVKZB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.036"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            411..1412
                     /locus_tag="VXA80_001025"
     CDS             411..1412
                     /locus_tag="VXA80_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0353538.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            1561..2478
                     /locus_tag="VXA80_001026"
     CDS             1561..2478
                     /locus_tag="VXA80_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0353539.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            2489..3379
                     /locus_tag="VXA80_001027"
     CDS             2489..3379
                     /locus_tag="VXA80_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0353540.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFKPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            3408..5003
                     /locus_tag="VXA80_001028"
     CDS             3408..5003
                     /locus_tag="VXA80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0353541.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYD"
     gene            5034..6353
                     /locus_tag="VXA80_001029"
     CDS             5034..6353
                     /locus_tag="VXA80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0353542.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(6563..6635)
                     /locus_tag="VXA80_001030"
     tRNA            complement(6563..6635)
                     /locus_tag="VXA80_001030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6600..6602),aa:Lys,seq:ctt)
     gene            complement(6900..8171)
                     /locus_tag="VXA80_001031"
     CDS             complement(6900..8171)
                     /locus_tag="VXA80_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV0353543.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     ISLSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSEHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            8441..8680
                     /locus_tag="VXA80_001032"
     CDS             8441..8680
                     /locus_tag="VXA80_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353544.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            8835..9023
                     /locus_tag="VXA80_001033"
     CDS             8835..9023
                     /locus_tag="VXA80_001033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353545.1"
                     /translation="MTWFEEIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFT
                     IDYTDDELLDKEAVFTTH"
     gene            9160..9339
                     /locus_tag="VXA80_001034"
     CDS             9160..9339
                     /locus_tag="VXA80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV0353546.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGE
                     LNKYTERGIRKQAGL"
     gene            9376..9828
                     /locus_tag="VXA80_001035"
     CDS             9376..9828
                     /locus_tag="VXA80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV0353547.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            10119..10589
                     /locus_tag="VXA80_001036"
     CDS             10119..10589
                     /locus_tag="VXA80_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV0353548.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEEGEFYPYPKY"
     gene            10608..11696
                     /locus_tag="VXA80_001037"
     CDS             10608..11696
                     /locus_tag="VXA80_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV0353549.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVVFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            11677..11835
                     /locus_tag="VXA80_001038"
     CDS             11677..11835
                     /locus_tag="VXA80_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV0353550.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     KSVRENLI"
     gene            11945..12979
                     /locus_tag="VXA80_001039"
     CDS             11945..12979
                     /locus_tag="VXA80_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0353551.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            12981..15224
                     /locus_tag="VXA80_001040"
     CDS             12981..15224
                     /locus_tag="VXA80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV0353552.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDGVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            15275..>15958
                     /locus_tag="VXA80_001041"
     CDS             15275..>15958
                     /locus_tag="VXA80_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV0353553.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLV"
BASE COUNT         5228 a         2561 c         3297 g         4872 t
ORIGIN      
        1 ctcatatact tgttaaatgc catgatgttt attcgattag atgagtaacc aaaaataaca
       61 aaaaaacgct atttcgcgtt tgaactgtac ctcaaaagtt agacagaaaa aatctaattt
      121 ttgggggtgt ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat
      181 ttatagccct ctaaaactct tgcttttttt gtagaagata cgaaaaaagc cataaaacca
      241 aggcttttcc tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta
      301 attggagtga ttttattcat gatgggataa atgatctggc caaaaatgat tgagaaatag
      361 ttgcttaaaa agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac
      421 aaagaaaatc aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg
      481 tttcacgtgt aattaataaa gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac
      541 aagcgattaa aactttgaat tacattccag attactacgc tagaggaatg aaaaaaaatc
      601 gaacagaaac gattgcaatc attgtaccaa gtatctggca tcccttcttt tcagaatttg
      661 ctatgcatgt ggaaaatgaa gtctataaga gaaataacaa attactctta tgttctatca
      721 atggtacaaa tagagagcaa gactatctgg agatgttgcg tcataataaa gttgatggag
      781 tggttgccat tacctatagt ccaattgaac attacttgac gtcaggaatt ccctttgtta
      841 gtattgaccg cacatactca gatattgcca ttccttgtgt ttcatcagat aatgatgcag
      901 gtggaagaga agcagctaaa caattaatta gtaaaggatg tcagcattta gcctttgtgg
      961 gcggacataa tacaaccatt aatgaaacca aacgtcgtag aatttcattt gaaaagtatg
     1021 tccaagagca taaaatacct cttagtattt ttgatttgga tgagacagtt actgactatt
     1081 atggaaagct agagagtttc ttggaagaaa attcttctat agatggaatt tttacaatca
     1141 acgattttac agctttggat gtgattgaaa tactagaaaa aaatggtaaa cgtattcctc
     1201 atgatgtcca aattattgga tacgatggaa ttaaaatggc tggagataga gattatctac
     1261 tttcaactat tgaacaacca ttggaagaaa tggcaaaaga agcggttcgt attttgtttg
     1321 atataattga tggaaagact gttaatttgc agacaatcct accagtaaaa tttgttgaag
     1381 gaaaaacaac aaaaaatgaa aataagtctt gacagaaagc gctatcaatg atagaatgaa
     1441 ttcagataaa aaagatttat ttttaaaaca aaaatgaaac gtttcaaaaa agaaataaag
     1501 agacagcgcc aagcgctatc ttttctagaa aaaaatgaaa cgtttcaaaa ggaggttgct
     1561 atgaatagca aagcgaagca agtttctctt tgggaaagaa tcaagaaaca aaaactcttg
     1621 ttattgatga ctgtccctgg tttagtttta acctttatct ttaaatacat ccctatgtat
     1681 ggggttttaa tcgcatttaa agattacaat cctttaaaag gaattttagg gagtgattgg
     1741 attggttttt ctgagtttac aaaattcata tcctctccca actttggtat cttgttagcc
     1801 aacacattaa aattaagtat ctatggttta ttgcttggct ttttaccacc aatcattctt
     1861 gctattatgc tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca gctcatttta
     1921 tacgcaccaa actttatctc agtcgttgtt attgtcggta tgattttcct cttcttttca
     1981 gtgggaggac caatcaacaa ttttctttct atgtttggaa tgaaggctga cttcttgaca
     2041 aatccagact tctttagacc tttatacatc tttagtggta tctggcaagg aatgggctgg
     2101 gcttcaacgc tctacacggc aacattggta aatgtagatc cagccttagt agaagcagcc
     2161 cgactggatg gagccaatat cttccaacga atctggcaca ttgatattcc agctcttaag
     2221 cctattatgg ttatccaatt tgttttagct gcaggtggaa ttatgaatgt cggatatgaa
     2281 aaagcattct tgatgcagac atcgttaaat ttgccaactt ctgaaattat ctcgacatat
     2341 gtctataaag ttggtcttgt atcaggagac tattcttact caacagcggt tggtttgttt
     2401 aatgcagtga ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa acgcatgaat
     2461 aatggtgaag gaatttaagg aggaaagtat gaaaaattcg attatggata caaaatttga
     2521 tagacgtatc ttactcttaa ataaaatcat tattgtcttt atcgttttga tgactttgct
     2581 tcctttactt tatatcgtcg tagcatcctt tatggatcct aaggttctgg ttagtagagg
     2641 gattagcttt aaaccagccg attggactgt agaaggttac cagcgtgtat tcagtgacca
     2701 atctattcta agaggtttta tcaattctct actatactct tttggatttg cagctttaac
     2761 agtattgcta tctgtgttta cagcttatcc tctttctaag aaagacttgg ttggacgtcg
     2821 ttggattaac tacttcttga ttgtgactat gttctttggt ggtggtttag tcccaactta
     2881 cttgctcgta aaagaattgg gaatgctcaa tactccatgg gctatcattg ttccaggtgc
     2941 tgtcaacgtt tggaatatta ttcttgctag ggcctatttc caaggattgc ctgaagaatt
     3001 agttgaagct gctgtcattg atggtgcaaa tgatttacag attttcttca aaatcatgct
     3061 tcctcttgca aaaccaatta tgtttgttct cttcctttat gcttttgtag gacagtggaa
     3121 ctcatacttt gatgcaatga tttatatcaa ggatccaaac ttggaaccat tgcaacttgt
     3181 acttcgtaaa attctcattc agagccaacc aggtcaagac atgattggag cacaagcggc
     3241 tatgaatgaa atgaaacgtt tagctgaatt gattaaatac gcaactattg tcatttccag
     3301 cttgccattg attgttatgt atccattctt ccaaaaatac tttgataaag gaattatggc
     3361 tggttcactt aaaggataaa aaaagaaaaa ataaaaggag ttttctcatg aaattcaaaa
     3421 cattctcaaa atcagcagtt ttgttgacag ctagtttagc agtacttgca gcctgtggct
     3481 caaaaaatac agcttcaagt ccagattata agttggaagg tgtaacattc ccgcttcaag
     3541 aaaagaaaac attgaagttt atgacagcca gttcaccgtt atctcctaaa gacccaaatg
     3601 aaaagttaat tttgcaacgt ttggagaagg aaactggcgt tcatattgac tggaccaact
     3661 accaatccga ctttgcagaa aaacgtaact tggatatttc tagtggtgat ttaccagatg
     3721 ctatccacaa cgacggagct tcagatgtgg acttgatgaa ctgggctaaa aaaggtgtta
     3781 ttattccagt tgaagatttg attgataaat acatgccaaa tcttaagaaa attttggatg
     3841 agaaaccaga gtacaaggcc ttgatgacag cacctgatgg gcacatttac tcattcccat
     3901 ggattgaaga gcttggagat ggtaaagagt ctattcacag cgtcaacgat atggcttgga
     3961 ttaacaaaga ttggcttaag aaacttggtc ttgaaatgcc aaaaactact gatgatttga
     4021 ttaaagtcct agaagctttc aaaaacgggg atccaaatgg aaatggagag gctgatgaaa
     4081 ttccattttc atttattagt ggtaacggaa acgaagattt taaattccta tttgctgcat
     4141 ttggtatagg ggataacgat gatcatttag tagtaggaaa tgatggcaaa gttgacttca
     4201 cagcagataa cgataactat aaagaaggtg tcaaatttat ccgtcaattg caagaaaaag
     4261 gcctgattga taaagaagct ttcgaacatg attggaatag ttacattgct aaaggtcatg
     4321 atcagaaatt tggtgtttac tttacatggg ataagaataa tgttactgga agtaacgaaa
     4381 gttatgatgt tttaccagta cttgctggac caagtggtca aaaacacgta gctcgtacaa
     4441 acggtatggg atttgcacgt gacaagatgg ttattaccag tgtaaacaaa aacctagaat
     4501 tgacagctaa atggattgat gcacaatacg ctccactcca atctgtgcaa aataactggg
     4561 gaacttacgg agatgacaaa caacaaaaca tctttgaatt ggatcaagcg tcaaatagtc
     4621 taaaacactt accactaaac ggaactgcac cagcagaact tcgtcaaaag actgaagtag
     4681 gaggaccact agctatccta gattcatact atggtaaagt aacaaccatg cctgatgatg
     4741 ccaaatggcg tttggatctt atcaaagaat attatgttcc ttacatgagc aatgacaata
     4801 actatccaag agtctttatg acacaggaag atttggacaa aattgcccat atcgaagcag
     4861 atatgaatga ctatatctac cgtaaacgtg ctgaatggat tgtaaatggc aatattgata
     4921 ctgagtggga tgattacaag aaagaacttg aaaaatacgg actttctgat tacctcgcta
     4981 ttaaacaaaa atactacgac taataccaag caaacaaaaa ctagaggttg attatgggag
     5041 ataagaaata cacagtagaa aaagccaatc gttttatagc agaaaataaa catctcgtta
     5101 atactcaata taagcctgaa gaacattttt cagctgagat tggttggatc aatgatccaa
     5161 atggatttgt ctattttcgt ggagaatacc atctctttta tcaattctat ccatatgata
     5221 gtgtttgggg gcctatgcac tggggacatg ctaaaagtaa ggacttggtg acttgggagc
     5281 acttgccagt ggcacttgct cctgaccaag attatgaccg aaatggttgt ttctcaggct
     5341 ctgccattgt caaggatgat cgcctctggc tcatgtacac tggacatatc gaagaagaaa
     5401 ccggtgtccg ccaagtgcaa aatatggcat tttcagatga cgggattcac tttgaaaaga
     5461 tttcccaaaa tccagttgca actggatcag acttgccaga tgagttgatt gctgctgatt
     5521 tccgtgatcc aaaactcttt gaaaaagatg gacgctatta ctccgtagta gctgccaaac
     5581 acaaggataa tgtgggctgt atcgttctac tagggtccga taacctagta gaatggcagt
     5641 tcgaatccat ctttttaaaa ggggtagaac accaaggctt tatgtgggaa tgcccagatt
     5701 acttcgagtt agatgggaaa gattgcctta ttatgtcacc catgcgttat cagcgtgagg
     5761 gagactcata tcataacatc aactcatcgc ttttgttcac gggtaaggta gattggagag
     5821 aaaaacgttt tatcccagaa tcagttcaag aaattgatca tggccaagac ttctatgcgc
     5881 ctcaaacatt gttggacgat caaaatcgtc gtatcctgat tgcttggatg cagacatggg
     5941 ggcgtaccct tccaacccat gaccaagaac acaagtgggc atgtgccatg actttaccta
     6001 gaattctaag attggaagat ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc
     6061 aaatccaaat agataaagat tgtcattacc acttaggaaa tgatacagat tatcttgaat
     6121 ttggttatga cagtaatgtg cagcaagttt acattgatcg tagccatctt attcaaaaaa
     6181 ttctaggtga agaagaacag gacactagtc gacggtatgt agatattgaa gctaaagaat
     6241 tggaagttgt tctagataaa aattccatcg agatttttgt caatcaaggt gaagcaagct
     6301 tgactgcaac ttattactta acggtgccag ctgagctatc acgaattgat taaaaattaa
     6361 gttatttctc ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga ctttttgtgt
     6421 tttgggtata taagcttagt ttatggtatt tgtaaaattg gtgttggatt atgatttaaa
     6481 ctagttttct aaagaatttg aaaaaaattt tatttaagca aaaaaagccc tatattataa
     6541 gacttttcct gttgtattta gatgccccct acagggatcg aacctgtgac ccacggatta
     6601 agagtccgct gctctaccag ctgagctaag gaggcaaaga aaaaagctgt attggtgccg
     6661 aaacttcacg gtttgtattg aacccgcgca attaagcagg tgggcaactc gctctaactg
     6721 aagctgtttc cgtgtgagac ggcctacatg ctgttagaag acttttgttt ccctaataat
     6781 acaaaaaata gtcggtcaac acttaagtgt gaagtcgtac accacagcgt ttctatactt
     6841 atatgatacc actttttcaa aaaaaatcaa gaggaaagtg caattttttg aaaaggattt
     6901 cagatttttc gcaaacggtc gatagcttcc tttctttgag ccaaagcccg ttcatattta
     6961 ccagtatctt ctgcttggaa atagtgatga ttacgaattt tttctggcag atagtcttgc
     7021 tttacccaat ttccaggata gttgtgtgga tagagatagt cttgggcatt ccccagttcc
     7081 ttgcttccac tgtagtgccc atcacgtagg tgtcgcggaa taggcaagtg ctctgatgtt
     7141 ttgaggtcag caagtgcctt atccatagct acataggctg agttggattt tggagaaagg
     7201 gccaaatcaa tcacgacatt ggcaatgaga atgcgggctt ctggaaaccc aatcttctgg
     7261 gcagcatcca gagcagtcac ggtatgaatc tgggcctcag gattggctaa gccgatatct
     7321 tcataggcga taacagtcaa gcgacgagcg agactaggca gatccccagc ctcaatcaag
     7381 cgggcagcat agtggagact ggcatccaca tctgagccac ggatagactt ttgcagggca
     7441 gatagaacat catagtgacc gtctccatcc ttgtccatag tgatgtagct tctctgaaga
     7501 ctattttcca tgatgtctaa ggtgatgtgg cgaatgccct cgtcattctc aggggtagag
     7561 agaacagcca agtccagtga gttaaaggca gagcgaaggt ctccgtttgt agaggttgcg
     7621 atgaaatcca gcgcatcctc atctagttct attggaaaat caaaaccacg ttcagggtta
     7681 cttagagata tctgcagggc ctctttgacg tcttggttag acagaggttc caactcgaaa
     7741 atttgaacac ggctacgaat ggcaggagtg acagagaaga aaggattttc agtcgttgct
     7801 ccaatcatga tgaccagtcc actttccaag agaggcaaga ggaagtcttg cttggtctta
     7861 tctagtcgat gaatttcgtc tagcaatagg acgagaccac cagaaaattt agcttcttcc
     7921 gagatttctt gcagtcgctt tttactatca actgtcgcat tgaaagttcg aaaggcatac
     7981 ttggtcgttc cagcgatggc agaggcaata ctggttttgc cgattcccgg agggccatat
     8041 agaatcatgg aggacaggcg gttggcttcc accatgcggc ggatgatttt tccaggtccg
     8101 accagatgct cctgaccgat gacctggtcg atggttttag ggcgcatgcg aagcgcgaga
     8161 ttgtctggca tagcagtcct ttctaacgtg gatttgagta gaatgactca tctactatat
     8221 cttactatgt ccattctagc ctattagttg tgatatttca atggttttgt aataatcagg
     8281 gagtgggata gaatcttttt tactatttaa tttaatgagg agaggtatca gcatgagaga
     8341 tttgattttc cttaaagctc aaaataggat agttgagcct aaagaagtgg aagtgctgac
     8401 ttgagaaata atattgctga atgtcattgt atgaactata atgaaaagag aggtgataag
     8461 caatggaaat gatggaccaa gtcaggaaat tttgattttt acaaaacaaa ttcgtcattg
     8521 gattcttagt gatcaagtta tttcagggaa aagaaaactt ttcttccggg aagatactcc
     8581 aaaagagatt ttagatttgt acgaaaacat taaatctaaa cttgattttg cttaccaaga
     8641 agttcattct aataatgggt tgaaaaaata tgaaaaatag aataattgat gtttttgaag
     8701 tagtaaatcg cctccttgta ctaactgttg aaaatcctga tttcgaagac ttgagagtta
     8761 atcatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt
     8821 acggtaaggc gacgttgacg tggtttgaag agattttcga agagtatcag tttgtaaaaa
     8881 ttggagataa aaaatataga gtacgcagtg ttcctatgat tcattcaact ccgcctcaat
     8941 ctgtcttaga tcgagatact tttacaattg actatacaga tgatgaattg ctcgataaag
     9001 aagcagtttt tactactcat tagaaaaata ggtatgaata aattggcttc ccttagaacc
     9061 acatctaggg gcttttatgt ataatagaat aataagtatc actcctttac aatacgtgta
     9121 acacgtgtta taatggtgtt gtcaggaggt aaaagcgcta tgcctatgac acaaaaagag
     9181 atggttaaac tccttacggc ccatggttgg ataaagacta gaggcggtaa aggttcccat
     9241 attaaaatgg agaagcaagg ggaaagacct atcacaatcc ctcatggtga gctgaataag
     9301 tacactgaaa gagggataag aaagcaagct gggttgtaaa attcagcccc tgctttttga
     9361 tatggaggta gtgaaatgtt agttacgtat ccagctttat tttattatga cgacacagac
     9421 ggaacagaag cgacttattt tgtccatttc ccagattttg aatactcagc tacacaagga
     9481 gaggggattt ctgaggcttt ggctatgggg tcggagtggc tagggataac tgttgcagat
     9541 ttgatagaga gtgatggaga gttacctcag ccatcagata tcaatagctt gtctttaatt
     9601 gataatgatc cttttaaaga tgatgaagat ttcgtgtcaa cctatgacct tgataaatct
     9661 tttatttcta tggtatctgt tgatgtatca gaatacctag gaagtcagga acccattaaa
     9721 aagactttga ctataccaaa atgggcagat aagttgggac gagaaatggg acttaacttt
     9781 tctcagactt tgacagacgc tattgcagat aagaaagttc aagcctaaga ttaagcgtgg
     9841 tatcgtggta atagtcaata aaaaagcacg tccacttgtg ctagttactc gcctattgat
     9901 tttatagatt tattagccct ttcgagggct ttttatattg atttttatga gaaatgaaga
     9961 aatcagactt ttaagaaaat cactgatacc aagggtttaa atgaggtaat atggtataat
    10021 taggacataa aataattttg tggtaagatg gtagtatcta ttttagcata tttccgagca
    10081 atcggggcga ttaaagagtc gcatagaaag aggacaaaat ggcaacatac ggatttttag
    10141 atgttttaga ggaagagttg gacaagaact ttccctttga ctttgagatt agctgggaca
    10201 aacgcaacca tgcggtagaa gtgagttttc tattggaagc gcaaaatgct gcaggtgtgg
    10261 agatggtgga tgaagacgga gaggtttcat cagatgacat tctttttgag gaagcggtcc
    10321 ttttctacaa tcctgccaag tcaacagtca atgaggaaga ctatttgact gttattcctt
    10381 acctgcctaa aaaaggattt tcccgtgaat ttttagctta ttttgcgctt ttccttaaag
    10441 atactgctga ggttgggcta gatgtcctca tggacttttt ggaagaccca gaagcagagg
    10501 aatttgtcat ggaatggaac caagaagtct ttgaagaagg aaaaattggc ttggaagagg
    10561 gagaatttta tccttatcca aaatactagg agttggttgg aggtgttatg aagaaaattg
    10621 ggacttattt ggtttatgtg ctagctgttg tctttattat gctagctttt gcttgtggaa
    10681 caatcgcatt tgcagagttg gggtattccg cagttttagc ctttactttt ggttatgcct
    10741 tcgctcttct aagcatgtat ttaatcttta ttcttcatga gctgggtcat gctttttgtg
    10801 gctacttgac aggctatcgg ctggtggttt ttggattagg acattttatt ttgaccaaaa
    10861 agtcaggcag gtttcatctt agtagaacag ccattctgaa aaatgttggt gctcaataca
    10921 ttggtttaaa agaggatgaa agcgatcaaa gaatcatcct gatgctttca ggaggcttga
    10981 tggttcatct cggcttgata ttattggcga tattgtttgg atttttgaca agaagctggt
    11041 attttgcagg gacttggatt tttcttaatt tatctttctt cctaaataac attttgccag
    11101 tcgacatcac tgatggagca aaaatttggg aattactaca acaccctgaa aataccaaat
    11161 acgcctacct ggtattgaga cattctgccc agaccttgct agctcctcaa gaatatgatt
    11221 tgaaagactt tgttcaagca gttcctgagg atgcacgagg gagttttgcg gatggtgtat
    11281 taggtatgca aggtgaagtt tccattttgg aagggaagga agaagtagct aaacagcagt
    11341 ttcaagcatt acttgagagg acaactactc cgatgatgca gacagttgcc caactatccc
    11401 tgcttcatat cgccttgttg gaaggagatt ttgagcaagc ggagcaatat gctagtattc
    11461 gacaggtcaa atcgtttctg tctctcaaac tgtccaatct tcaagtgata caagcctggt
    11521 atcaattaaa ggtgaagaaa gatgtagatc gaactcgtaa ggctctcaag attgctagac
    11581 agaaaatgga tagtagtcga atgttgaggg acgagaaacg ctattatgaa aacttgttag
    11641 cagagctgga aaaggaaatg ttagaaggaa ctgaagatgg aactagaaat ttctgatttc
    11701 caaggttgca agattgccct cttttgtggg gataagctac tgacgatttt gcgtgatgac
    11761 aaggacgata tcccttgtcc caacatgtgg gagttgccag gtggtggaaa gagtgtcagg
    11821 gaaaatctga tatagttttg cattaaaaga atatcctttc aaggttattc ttttttaatt
    11881 ttgtcccaaa ataattgact ttgtcccgaa aatatagtat tatttgtata aggaggaatg
    11941 aatcatgaaa aaatcagatg ttcttgattt aataaagtat cattatgagg gcagagagac
    12001 agaatttaga aatcaatcca tagcaattgc tcggaacttc aataaacatg gtgatacaca
    12061 aatagcacaa tatattatgg ggttaatgtc tcaaagtgat agattcatgc cacaaataga
    12121 aaatcctagt gagtatttga ctcctgctaa actagatatt ggtcctctcc cattgcctct
    12181 ttcaattatg aatgatttaa aaggaataat aaacgctgtc aatcatcata ttggaataaa
    12241 taaattttta tttgtgggat ctccaggtac tggaaaaact gaaagtgtaa agcaggtggc
    12301 tagacttatt ggcaaggaat tacttgtagt agatttcagt cacttagtag atagtaagct
    12361 agggcaaaca gtcaaaaact tagcaactct ttttaatgaa atcaataatc ttcctttcaa
    12421 gcaaaattat atcatcctat ttgatgaaat tgattccata gtgttggata gggttaatca
    12481 gaatgattta agagaaatgg gacgagtgac ttctgccttt ttaaaggagt tggataggct
    12541 gtcaccagaa attgtattaa ttgcaacaac aaatcttttt gaaaatcttg ataaagcagt
    12601 tacgagaaga ttcgatgcca taattgactt tgaccgttat actgatgaag ataaagtaga
    12661 agtagctact attattttaa atgagttatt aaagcaattt aagaatgtag ctagagattt
    12721 aaaattattt aaaaaaatta tcaatagtgc caatgtaata ccgaatcctg gtgatttgag
    12781 aaactcaata agaacgtcat tggcatttag tgacccatca gatccgcatg attaccaaaa
    12841 acgcctgcta agaagtttgc ataatggtag aaatttatct atttctaaat tatcgaagct
    12901 aggttttact gttagagaaa ttgagatttt gacaggtatt tctaagagta gtgtatcacg
    12961 agagttaagc gaggattaaa atgaatacat tattgacatt acgaggtaaa agttttacac
    13021 caaagtcagc aaaggggagg ggaccgataa cgattcccgc agattctatt gttacgctaa
    13081 accatttaaa tgaactttat acatcccttg aacgagtaaa agaatattgg tctaataaca
    13141 caatcattga tggagtactt attagtgtgt attataatcg tgtcctagct aagagcaatc
    13201 gtatcgatgg tttttttaat gtgggtggcg gaaatgcaat tccaaatgaa acgattgtgg
    13261 gtgcaaaatt tagtaatgat aagagtaggc atattattac acactacatt tcaaaggaag
    13321 tgttagataa gacaattgag catactaaaa aagttataac aatattcaaa gattattttg
    13381 ctagtgctcc catgacgagt atggtgttct ccgactcttc aaaatttaat caaattgatt
    13441 attctaagta tggtctgtcg aaatcaaaat tcaaacaata cttaagagac gcttgctttg
    13501 tcaaaggatt tggtattgaa caggcagata tgtctactat acgtaattct attgtaacaa
    13561 tctacgatgt aaaaactaat ctcttaacat tacttaaaca acttgatatt gatgtttcag
    13621 aagctaacat catggaccag acaactgttt tacttgacga aaaaaatatt gagttactac
    13681 tgctaaaagc accttatcta gtatcaatga tagtagaaga tttttctagc ctgtcaccag
    13741 agaattttta taatgatggg aacacttttt ataataatct cccttttcca actaatgaac
    13801 ctattattgg tgtcatcgat acgttgtttg atgatggagt ttatttcaat gagtgggtag
    13861 agtatcatga tttaatttca ccagatatta gcaaaggaag tcaagactat aagcacggta
    13921 cagcagtttc ctcggtaata gtagatggag ctagtttgaa tccaaaacta gatgacggat
    13981 gtggaagatt tagagtcaga cattttggga tatctttaca gagtggtttc aattccttca
    14041 caattatcaa acagattaga gaaattgtgt ctcaaaatcc tgatattaag gtttggaacc
    14101 tttcgcttgg atcaaatgat gaaattaggg aaaactttat ttccgtcgag ggttcgttgt
    14161 tagacgagat tcaatttgat aacgatgtaa tttttattat tgcaggcact aatgggattg
    14221 cccctgatgg acaacgaaaa aagattggag caccagcaga ttcactcaat tcaattattg
    14281 taaattctgt tgattttagc aatcaagtcg tttcttattc acgcgaagga gttgtcctgt
    14341 ctttctttat caaacctgat gtttcatact atggcggggg gaacggagac ttcataaatg
    14401 tttgtgaacc actagggctg gctcgagtag ctgggaccag ttttgcggct ccatttattg
    14461 ctagaaaaat ggcttacctc attcatatta tgggattaag tcgtgaagaa gcaaaggctt
    14521 tattaattga ttcagcgata ccatggaatg ataaaaaaac attcgcagac atttctttac
    14581 ttggatatgg ggttgtgcca atcaaaatag aagatatttt gtctacacct gatgatgaaa
    14641 ttaaatttat agtctcagat gtttctagag catatgaaac ttataattat gatttcccag
    14701 ttccaatttc taaagaacac tatccatatg tagcaaaggc cactatgtgc tattttccaa
    14761 agtgttctag aaatcaagga gttgactata ctaatacgga aatgcaactt agtttgggag
    14821 ttttagataa gaaaggcatt aaaacaatta ataacgacaa tcaatattct gaaaatgcac
    14881 caggatatgt gagagaaaag tcggcacgaa atatttttag gaaatgggat aatgtcaaac
    14941 atttaggaga aaaattttct tctcgtaaaa aagctaagcc gattctgaat ccttctaatc
    15001 ctcaatgggg aatgagtgta aaaacaattg aacggttaag aaatgatgat ggttatggta
    15061 ttcgttttgg agtagttgtt actctaaagg agttgaatgg tgtcaataga atcgaagatt
    15121 ttattcaaca agccgaatta cgcggttggc tagttaatag attgcaagta gaagctcaag
    15181 tagacctctt taatagttta aatgaagaaa ttgaatttga atagtggata ttttatcctt
    15241 attctagatc ttaggaacac aagagggcaa agatatgaat attacttata tagttggaaa
    15301 tggtttggat ttgcaatatg gtttgaaaac aagatataaa gatttctatg aatttcaaaa
    15361 taggacttac ataagtagga aagagaacga ggaagaatat tcaaatctca tttatgagtc
    15421 tctattttct gatacagtaa atgattatga aaattggtca gattttgaat tgtcaattgg
    15481 taaattaaca aaagataacg acctcatctc ctcaagtata gaaataaagg agaaatttat
    15541 agatgatttt agtgaagtag ttgatgattt aagagaatat ctacgcatac aacaagaaaa
    15601 aaatcttgag aaaggtaatg caattgactt tataagcact ctagataata tgaggacctc
    15661 tctccctgta attaatcaac ctgctatcga taaaaaatat aatgaaaatc tatatcaaca
    15721 cgatatcgtg aatatattaa ctcttaatta cacaaatgta atcgataaat tatataatga
    15781 gtcagcaaaa tcatttagca atcaattacg aactaataat tataaatttc acattgcgcc
    15841 tccaattcat gcacatggaa cggtagatat ttgcacagtt ttaggagtta gtgacgagac
    15901 acagatttca aatagttttg atgaagaaca aaaagaatcc ttaataaaaa atttagtt
//
LOCUS       DAVKZB010000037        15859 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKZB010000037 DAVKZB010000000
VERSION     DAVKZB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.037"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            252..599
                     /gene="ssrA"
                     /locus_tag="VXA80_001042"
     tmRNA           252..599
                     /gene="ssrA"
                     /locus_tag="VXA80_001042"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(782..869)
                     /locus_tag="VXA80_001043"
     tRNA            complement(782..869)
                     /locus_tag="VXA80_001043"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(833..835),aa:Ser,seq:gga)
     gene            1031..1771
                     /locus_tag="VXA80_001044"
     CDS             1031..1771
                     /locus_tag="VXA80_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0353554.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSIKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1768..2679
                     /gene="pfkB"
                     /locus_tag="VXA80_001045"
     CDS             1768..2679
                     /gene="pfkB"
                     /locus_tag="VXA80_001045"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV0353555.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINIKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2676..4628
                     /locus_tag="VXA80_001046"
     CDS             2676..4628
                     /locus_tag="VXA80_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353556.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4792..7017
                     /locus_tag="VXA80_001047"
     CDS             4792..7017
                     /locus_tag="VXA80_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV0353557.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7216..7641)
                     /locus_tag="VXA80_001048"
     CDS             complement(7216..7641)
                     /locus_tag="VXA80_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353558.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQPNTPTSENNTQ"
     gene            7779..8921
                     /locus_tag="VXA80_001049"
     CDS             7779..8921
                     /locus_tag="VXA80_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0353559.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8930..10144
                     /gene="thiI"
                     /locus_tag="VXA80_001050"
     CDS             8930..10144
                     /gene="thiI"
                     /locus_tag="VXA80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV0353560.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10279..11103
                     /locus_tag="VXA80_001051"
     CDS             10279..11103
                     /locus_tag="VXA80_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0353561.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            11125..11864
                     /locus_tag="VXA80_001052"
                     /pseudo
     CDS             11125..11864
                     /locus_tag="VXA80_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11877..13043
                     /locus_tag="VXA80_001053"
     CDS             11877..13043
                     /locus_tag="VXA80_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0353562.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            13551..15044
                     /locus_tag="VXA80_001054"
     CDS             13551..15044
                     /locus_tag="VXA80_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0353563.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTSRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15057..>15859
                     /locus_tag="VXA80_001055"
     CDS             15057..>15859
                     /locus_tag="VXA80_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0353564.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAY
                     GGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKE"
BASE COUNT         4740 a         2934 c         3513 g         4672 t
ORIGIN      
        1 agtacgaaaa ttctcatctg aaagtatttt agaataattc tcttcgtgaa tttcttcaaa
       61 acagatagct tcatcttagg tatgtgttaa ttggatttcg taagctctat ttacaatcgt
      121 aaagttatgc actgaaaaag ttgtagttag tcttccagtt tgctaaattt ctcgaatagt
      181 atctttttta ggtatgtgat ttctttttgc atttttgagt tagataaggt ataatggttt
      241 tattgtcttt tggggtcgtt acggattcga caggcattat gaggcatatt ttgcgactcg
      301 tgtggcgacg taaacgctca gttaaatata actgcaaaaa ataacacttc ttacgctcta
      361 gctgcctaaa aaccagcagg cgtgacccga tttggattgc tcgtgttcaa tgacaggtct
      421 tattattagc gagatacgat taagccttgt ctagcggctt gataagagat tgatagactc
      481 gcagtttcta gacttgagtt atgtgtcgag gggctgttaa aataatacat aacctatggt
      541 tgtagacaaa tatgttggca ggtgtttgga cgtgggttcg actcccaccg gctccattat
      601 tcctttgcat tcttttgcat tccttggtaa aacgttgtta aatcaacgtt ttttattttt
      661 atctttggta ttcctttgca ttcttttgct aaaaagggag tcacaaacag accctatttt
      721 aaaaaaagga tagaaaaaag gatacaacat ttgtcgcatc ctaaaaataa tcttttttcc
      781 acggaagaca tgggattcga acccacgcac gctattacac gcctaccgcg tttccaacac
      841 ggcctcttaa gcctcttgag taatcttcca atacttactc aaatagtcta ccataaaggc
      901 tcttatcttg caataaaaat tctagaaata agaaaaatga tagattttga aagaaaatga
      961 taaaaaatgc ttgacttcga aataaagtat ggtagaatga atagtgtaaa cgataacagg
     1021 aggtgattca gtgttaaaaa cagaacgtaa acaactgatt ttagaggagt taaatcaaca
     1081 tcatgtagtt tctctagaaa aattagttag tttgctagaa acgtcagaat caacggttcg
     1141 aagagacttg gatgagttgg aagcggaaaa caagcttcgt cgtgtgcatg gtggagcaga
     1201 actcccccac tccttacagg aagaagaaac cattcaagaa aaatctatca aaaaccttca
     1261 agaaaagaaa ttgctggctc agaaagcagc ctctctcatt aaagaaaaag atgtcatctt
     1321 tatcgatgct ggaacaacaa ctgctttttt gattcatgaa ttggtcaata agaatgttac
     1381 agttgtgacc aactccattc accatgccgc tcagttggtt gaaaagcaga ttccaactgt
     1441 catggttgga ggaaacgtca agacggcgac agatgctagt atcgggggcg ttgctcttaa
     1501 ccagattaac caattgcact ttgaccgtgc ctttatcgga atgaatggtg ttgacgatgg
     1561 ctattatacg actcctgata tggaggaggg agctgtgaaa agagctattt tggagaatgc
     1621 caagcagacc tacgtcttgg tggattcgtc aaaaattgga caaacttgct ttgccaaggt
     1681 agccccactc aaacgcgcta tcgttatcac tagtcaaggg catgagctct tgcaggttat
     1741 taaggagaaa acggaggtaa tagaagtatg atttatacag tcacactcaa tccatccatt
     1801 gactatatcg ttcgtttgga ccaagtcaaa gttggtagtg taaatcgtat ggacagtgat
     1861 gataagtttg ctggtgggaa aggaatcaat gtcagccgtg tcttgaaacg tttgaatata
     1921 ccaaatacag cgacgggatt tatcggtggc tttactggta aatttatcac agatacttta
     1981 gcagaggaag aaatcgagac acgttttgtc caggtggcag aagatactcg tattaatatt
     2041 aaaatcaaag cagaccaaga aacagaaatc aacggaacgg gtccaactgt tgaaccggtt
     2101 cagctagaag aattgaaagc tattttatct agtctgacag cagaagatac agttgtcttt
     2161 gcaggttcaa gtgctaaaaa tctaggcaat gttatctata aggatttgat ttccttgacg
     2221 cgccagactg gtgcgcaagt ggtctgtgac tttgaaggac agaccttaat tgatagtttg
     2281 gactaccagc ctcttcttgt aaaaccaaac aatcatgaac ttggagcgat ttttggggtt
     2341 aaactcgaaa gtttagatga aattgagaaa tacgctcgtg agttactggc taagggtgct
     2401 caaaatgtta ttatctctat ggctggtgat ggcgcccttc ttgtcacatc tgagggagct
     2461 tacttcgcta aaccaatcaa aggaacagtc aaaaattcag ttggagctgg tgattctatg
     2521 gttgctggat tcacaggtga atttgtcaaa tcaaaagacg cagtagaagc cttcaaatgg
     2581 ggagtggctt gcggaacggc aactaccttc tcagatgact tggcaacggc ggaatttatt
     2641 aaagaaacat atggaaaagt tgaggtagaa aaacgatgaa aattcaagat ttattgagaa
     2701 aagatgtcat gttgctggat ttgcaggcaa ctgaaaaaac agctgtcatc gacgagatga
     2761 ttaaaaattt gacagagcac ggttatgtaa cagattttga aacatttaaa gaaggaattt
     2821 tggcgcgtga agctttgact tctactggtt tgggtgatgg aatcgcaatg cctcacagca
     2881 aaaacgctgc tgtcaaagaa gcgacagttc tatttgctaa gtcaaataag ggtgttgact
     2941 acgagagctt ggatggacaa gcaactgacc tcttcttcat gattgcagct ccagaaggtg
     3001 ccaatgatac tcacttggca gccttggcag aattgtctca atacttgatg aaagacggtt
     3061 ttgcagacaa acttcgtcaa gcaacatctg cagaccaagt tatcgaactt tttgaccaag
     3121 cttcagaaaa aactgaggaa cttgttcaag cacctgctaa tgactctggt gactttatcg
     3181 tagctgttac agcttgtaca acaggtattg cccacactta catggcccaa gaagcccttc
     3241 aaaaagtagc tgctgaaatg ggggttggta tcaaggtcga aaccaacggt gctagcggtg
     3301 ttggaaatca actaactgca gaagatatcc gtaaggctaa agctattatc attgcagcag
     3361 acaaggccgt tgaaatggat cgatttgatg gaaaaccatt gatcaatcgt ccagttgctg
     3421 acggtatccg taagacagaa gagctaatta acttggctct ttcaggagat actgaagtct
     3481 accgtgccgc taatggtgca aaagctgcaa cagcctctaa cgaaaaacaa agccttggtg
     3541 gtgccttcta caaacacttg atgagtggtg tatctcaaat gttaccattc gttatcggtg
     3601 gtggtatcat gattgccctt gccttcttga ttgacggtgc tttgagtgtt ccaaatgaaa
     3661 accttggcaa tcttggttct taccatgagt tagcttctat gtttatgaaa attggtggag
     3721 ctgcctttgg tttgatgctt ccagtctttg cgggttatgt tgcctactct attgctgaaa
     3781 aaccaggttt ggtagcaggt ttcgtggctg gtgctattgc caaagaaggt tttgcctttg
     3841 gtaaaattcc ttatgccgca ggtggtgaag caacttcaac tcttgcaggt gtctcatctg
     3901 gtttcctagg tgcccttgtt ggtggattta tcgcaggtgc cttggttctt gccatcaaga
     3961 aatacgttaa agttcctcgt tcactcgaag gtgctaaatc aatccttcta ttgccacttc
     4021 ttggaacaat cttgacagga tttgttatgc tagctgtaaa tatcccaatg gctgcaatca
     4081 acactgctat gaatgacttc ctaggcggtc ttggaggagg ttcagctgtc cttcttggta
     4141 tcgtccttgg tggaatgatg gctgttgaca tgggtggacc agttaataaa gcagcttatg
     4201 tttttggtac aggtacgctt gcagcaactg tttcttcagg tggttctgta gccatggcag
     4261 cagttatggc tggaggaatg gtgccaccac ttgcaatctt tgtcgcaact cttcttttca
     4321 aagataaatt tactaaggaa gaacgtaact ctggtttgac aaacatcatc atgggcttgt
     4381 catttatcac tgagggagcg attccatttg gtgccgctga cccagctcgt gcgattccaa
     4441 gcttcatcct tggttcagca gtagcaggtg gactcgttgg tcttactggt atcaaactca
     4501 tggcgccaca cggaggaatc ttcgttatcg cccttacttc aaatgctctc ctttacctcg
     4561 tttctgtctt ggtaggagca atcgtaagtg gtgtggttta tggttaccta cgcaaaccac
     4621 aagcataaaa aatagaaaaa tgaaaagatt ggaccgtttg gtgcagtctt tttctcttcc
     4681 cgaaatgcct gtgaaatatg gtataataga agaatggcaa acaagaatac aagtacaaca
     4741 agacggagac cgtctaaagc agaactggaa agaaaagaag cgattcaacg aatgttgatt
     4801 tcgttaggaa ttgcgatttt attgattttc gcagccttca aattaggggc tgcaggtata
     4861 accctttata atttaattcg cttgctagtg ggtagcctag cttatctggc gatattcggc
     4921 ctattaatct atctcttctt tttcaagtgg atacgaaaac aggaaggact cttatctggc
     4981 tttttcacca tatttgctgg cttactcttg atttttgagg cctacttggt ttggaaatat
     5041 ggtttggaca agtccgttct aaaagggacc atggctcagg ttgtgacaga tctgactggt
     5101 tttcgaacga ctagttttgc tggagggggc ttgatcgggg tcgctcttta tattccaaca
     5161 gcctttctct tttcaaatat cggaacttac tttattggtt ctatcttgat tttagtgggt
     5221 tctctcctag tcagcccttg gtctgtttac gatattgctg aatttttcag tagaggcttt
     5281 gccaaatggt gggaagggca cgagcgtcga aaagaggaac gctttgtcaa acaagaagaa
     5341 aaagctcgcc aaaaggctga gaaagaggct agattagaac aagaagagac tgaaaaagcc
     5401 ttactcgatt tgcctcctgt tgatatggaa acgggtgaaa ttctgacaga ggaagctgtt
     5461 caaaatcttc cacctattcc agaagaaaag tgggtggaac cagaaatcat cctgcctcaa
     5521 gctgaactta aattccctga acaggaagat gactcagatg accaagatgt tcaggtcgat
     5581 ttttcagcca aagaagccct tgaatacaaa cttccaagct tacaactctt tgcaccagat
     5641 aaaccaaaag atcagtctaa agagaagaaa attgtcagag aaaatatcaa aatcttagaa
     5701 gcaacctttg ctagctttgg tattaaggta acagttgaac gggccgaaat tgggccatca
     5761 gtgaccaagt atgaagtcaa gccggctgtt ggtgtaaggg tcaaccgcat ttccaatcta
     5821 tcagatgacc tcgctctagc cttggctgct aaggatgtcc ggattgaagc accaatccct
     5881 gggaaatccc taatcggaat tgaagtgccc aactccgata ttgccactgt atctttccga
     5941 gaactatggg aacaatcgca aacgaaagca gaaaatttct tggaaattcc tttagggaag
     6001 gctgttaatg gaaccgcaag agcttttgac ctttctaaaa tgccccactt gctagttgca
     6061 ggttcaacgg gttcagggaa gtcagtagca gttaacggca ttattgctag cattctcatg
     6121 aaggcgagac cagatcaagt taaatttatg atggtcgatc ccaagatggt tgagttatct
     6181 gtttacaatg atattcccca cctcttgatt ccagtcgtga ccaatccacg caaagccagc
     6241 aaggctctgc aaaaggttgt ggatgaaatg gaaaaccgtt atgaactctt tgccaaggtg
     6301 ggagttcgga atattgcagg ttttaatgcc aaggtagaag agttcaattc ccagtctgag
     6361 tacaagcaaa ttccgctacc attcattgtc gtgattgtgg atgagttggc tgacctcatg
     6421 atggtggcca gcaaggaagt ggaagatgct atcatccgtc ttgggcagaa ggcgcgtgct
     6481 gcaggtatcc acatgattct tgcaactcag cgcccatctg ttgatgtcat ctctggtttg
     6541 attaaggcca atgttccatc tcgtgtagca tttgcggttt catcaggaac agactcccgt
     6601 acgattttgg atgaaaatgg agcagaaaaa cttcttggtc gaggagacat gctctttaaa
     6661 ccgattgatg aaaatcatcc agttcgtctc caaggctcct ttatctcgga tgacgatgtt
     6721 gagcgcattg tgaacttcat caagactcag gcagatgcag actacgatga gagttttgat
     6781 ccaggtgagg tttctgaaaa tgaaggagaa ttttcggatg gagatgctgg tggtgatccg
     6841 ctttttgaag aagctaagtc tttggttatc gaaacacaga aagccagtgc atccatgatt
     6901 cagcgtcgtt tgtcagttgg atttaaccgt gcgacccgtc tcatggaaga gcttgagatg
     6961 gcaggtgtca ttggtccagc tgaaggtacc aagccaagaa aagtattgca gcaataaaaa
     7021 atagcttctt tccaaggttg gaaggagcta ttttactggt tattgattac ttttatactc
     7081 ttcgcaaatc tcttcaaacc acgtcagctt cgccttgtcg taggtatatg atactgactc
     7141 tgtcagttct atccacaacc tcaaaacagt gttttgagca acctgcggct agcttcctag
     7201 tttgctcttt gattttcatt gagtattatt ttctgaagtt ggagtattgg gctgtttttc
     7261 gttttcagta gcaggtttac ttgaagcagg agtagaagag tcctgagttg ctgttttctg
     7321 atcttctttt ttctcttcct tgacgctaga ttttggtgtt tcctcttgct gtgttttttc
     7381 ttgactagtg ttagtctctt tagttggact ggtgttttcc ttaggggatt ccttttggat
     7441 ttctttgaca atggttgtcg tctggcttgt cgtaggttct tttttaatat ttttgttatt
     7501 atccaaggcg ttcataatcg tgatacttgc ggtaatcaag ccaaggatac tagcgattgt
     7561 agcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg tgttctgttt ttggttttga
     7621 agttctacga tagttagaca tacattctct cctttgatta tgatttatta tactctgttt
     7681 ctttaaaaaa atcttaaaaa aagagaaatt tccctttctt gtcgcaagct ccgtttcatg
     7741 atacaataga ggaagtgatt ttagaaagcg tgagaaacat gatttacttt gataattcag
     7801 cgacgaccaa gccttatcct gaagcccttg aaacctatat gcaggtcgct tcaaaaattt
     7861 taggaaaccc atctagtctc catcgtttgg gagaccaagc aacacgaatc ttagatgctt
     7921 ctcgccaaca gattgcagat ttaatcggta agaaaagcga tgaaatcttc tttacctcgg
     7981 gtggaacaga aggggataac tggcttatca agggtgtggc ctttgaaaaa gctcagtttg
     8041 gcaagcacat cattgtttca gccattgaac atccagcagt caaagagtca gccctctggt
     8101 tgaaaagtca aggatttgaa gtggattttg ctccagttga taagaaaggc ttggtcgatg
     8161 ttgaggcgtt agcaggtttg atacggcatg atacaatcct cgtttccatc atggctgtga
     8221 acaatgaaat cggctctatc caacctattg aggctatttc agaattcttg gcagacaagc
     8281 cgactatttc cttccacgtt gatgcggttc aggcgcttgc caaaattccg actgaaaagt
     8341 atctgacaga acgggtggat tgcgcgactt tctctagtca caagttccac ggggttcgag
     8401 gtgttggctt tgtctatatc aaatctggca agaagattac acctcttctt acaggtggtg
     8461 gccaggagcg agattatcgt tcgacaactg aaaatgtggc agggattgca gcgacagcca
     8521 aggccctccg tttgtctatg gaaaagctag atatctttag gagcaagact gggcagatga
     8581 aggcagtgat tcgccaagct cttctgaact atccggatat ttttgtcttt tcagatgagg
     8641 aaaactttgc acctcatatt ctgacttttg gaatcaaagg tgttcgaggt gaagtcatcg
     8701 ttcacgcctt tgaagactat gatattttca tctcaacaac ctcagcttgt tcatctaagg
     8761 caggaaaacc agccggtacc ttgattgcca tgggagtgga caaagataag gccaagtcag
     8821 ctgtgcgtct tagcctagac ttggaaaatg atatgagtca ggtcgagcag tttttgacca
     8881 agttaaaatt gatttacaat caaactagaa aagtaagata ggagcattca tgcagtattc
     8941 agaaattatg attcgctacg gagagttgtc aaccaagggt aaaaaccgta tgcgtttcat
     9001 caataaactt cgtaataata tttcggacgt tttgtctatc tatacccaag ttaaggtaac
     9061 agcagatcgc gaccgtgccc acgcttacct caatggagct gattacacag cagttgcaga
     9121 atctctcaaa caagtttttg gaattcaaaa cttttctcct gtttataagg ttgaaaaatc
     9181 tgtagaagtt ttgaagtctt ctgtccaaga gattatgcgg gacatctaca aggaaggtat
     9241 gacctttaag atttctagca agcgtagcga ccacaacttt gaacttgata gtcgtgaact
     9301 caaccaaaca cttggagggg ctgtattcga agccattcca aatgtgcaag ttcaaatgaa
     9361 aagtcctgac atcaatcttc aggtggagat tcgtgaagaa gcagcctatc tttcttatga
     9421 aaccattcgt ggggctggtg gtttgccagt tggaacttca ggtaaaggga tgctcatgtt
     9481 gtcaggaggg attgactcac ctgtagcagg ttatcttgct cttaagcgtg gggtggatat
     9541 cgaggcagtt cactttgcta gtccaccata tactagtcct ggtgccctca agaaagcgca
     9601 ggacttgacc cgtaaattga ccaagtttgg cggaaatatc cagtttatcg aggtgccttt
     9661 cacagagatt caagaggaaa tcaaagccaa agcgccagaa gcttatttga tgactctaac
     9721 tcgtcgcttt atgatgcgga ttactgaccg tattcgtgag gtacgaaatg gtttggttat
     9781 catcaatggg gaaagtctag gtcaagtagc cagccaaacc cttgaaagta tgaaggctat
     9841 caatgctgtt accaacactc ccatcattcg tcctgtggtt accatggaca agttggaaat
     9901 tattgacatc gcccaggaaa tcgatacctt tgacatttca atccaaccgt ttgaagactg
     9961 ttgtaccatt tttgcaccag atcgtccaaa aacaaatcct aaaattaaga atgcggagca
    10021 gtacgaagcg cgtatggatg ttgaaggctt ggttgagcga gcagtggctg gaatcatgat
    10081 tactgaaatc acacctcaag ccgaaaaaga tgaagttgat gacttgattg acaatctgct
    10141 ctaattcaga aaatccaaaa gaatagcgaa aatcagtaaa aaaagttagt tttttctcta
    10201 aaaacaggta aaaaactaac tttttttatt tttatgatat aatgatataa aattttgaat
    10261 atagagagtt ttctgacaat gaatcaatcc tacttttatc taaaaatgaa agaacacaaa
    10321 ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc taaagattat
    10381 gagaaagata cagaccgttc ctatcctgtt gtatactttc atgacgggca aaatgttttt
    10441 aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat caaacgaaat
    10501 ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg gcggatgaat
    10561 gagtatgcgg cttggaagtt ccaagaatct cctatcccag ggcagcagtt tggtggtaag
    10621 ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga tgagacctat
    10681 cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg aggcaatatt
    10741 acccagttta tcggtttgga ataccaagac caaattggtt gcttgggcgt tttttcatct
    10801 gcaaactggc tccaccaaga agcctttaac cgctatttcg agtgccagaa actatcgcct
    10861 gaccagcgca tcttcatcta tgtaggaaca gaagaagcag atgatacaga caagaccttg
    10921 atggatggca atatcaaaca agcctatatc gactcgtcgc tttgctatta ccatgatttg
    10981 atagcagggg gagtacatct ggataatctt gtgctaaaag ttcagtctgg tgccatccat
    11041 agtgaaatcc cttggtcaga aaatctacca gattgtctga gattttttgc agaaaaatgg
    11101 taagttaaga aaggaaaaaa cgaaatgcat attgaacatc ttagccactg gagtggtcat
    11161 cttaaccgtg aaatgtacct taaccgttat ggacatggtg ggattccagt tgtggtcttt
    11221 gcttcatcag gtggtagtca caacgaatac tatgattttg gcatgattga tgcctgtgct
    11281 tcctttatcg aggaaggcct tgtccagttc tttaccctat ctagtgtgga tagtgagagc
    11341 tggttggcta cttggaaaaa tgctcatgac caagcggaaa tgcaccgtgc ctacgaacgt
    11401 tatgtgattg aggaggccat tcttttatca agcacaagac aggttggttt gatggcatga
    11461 tgacgacagg ttgctctatg ggagcctatc atgcactcaa tttcttcctc cagcatccag
    11521 atgtctttac caaagtgatt gctctcagtg gtgtttacga cgcacgtttc tttgtcggtg
    11581 attactacaa cgatgatgct atttaccaaa actcgccagt agattatatt tggaaccaaa
    11641 acgacggctg gtttattgac cgttaccgtc aggcagagat tgtgctgtgt acggggcttg
    11701 gagcctggga acaagatggt ttgccatcct tttacaagct caaagaagcc tttgacaaga
    11761 aacaaattcc agcctggttt gctgaatggg gacatgatgt cgcccatgac tgggaatggt
    11821 ggcgtaaaca aatgccttat ttcctcggta atctctattt ataaaaggag ttacctatga
    11881 attaccttgt tatttctccc tactatccac aaaactttca acagtttacc atcgaactag
    11941 ctaataaagg catcacagtc ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc
    12001 ccttgcgcaa tagcttgacc gagtattttc gtgttgataa tcttgagaac atagatgaag
    12061 tcaaacgtgc agttgctttt ctcttttata aacatggtcc aattggccgc atcgagtctc
    12121 acaatgaata ctggcttgag ctagacgcaa cactcagaga acaattcaat gtttttggtg
    12181 ccaaaccaga ggatctcaaa aagacgaaat ataagtctga aatgaagaaa cttttcaaaa
    12241 aagcaggtgt tcctgtggta cctggagctg ttatcaagac ggaagcagat gttgatcaag
    12301 cagtgaaaga aatcggtctt ccaatgattg ccaaacctga taatggagtg ggagcagccg
    12361 caacctttaa acttgagaca gaagacgata tcaatcactt caagcaagaa tgggaccatt
    12421 caacccttta tttctttgaa aaatttgtca cttccagcga aatctgtacc tttgacgggc
    12481 tcgtggacaa ggatggaaag attgtcttct caacaacctt tgactacgcc tatacaccgc
    12541 ttgacctcat gatttataag atggacaatt cttattatgt gctcaaggat atggatccta
    12601 aactgcgcaa gtatggggaa gcaattgtca aagaatttgg tatgaaagaa cggtttttcc
    12661 atattgagtt cttccgtgag ggggacgatt atattaccat cgagtacaat aaccgccctg
    12721 caggtggttt taccattgat gtttataact ttgctcattc cttggacctt tatcgtggct
    12781 atgcagctat tgtcgcagga gaggagttcc cggcgtcaga ctttgaaact cagtattgtt
    12841 tggctacttc tcgccgtgca aatgctcact atgtttattc agaagaggat ttgcttgcca
    12901 aatatagcca gcagttcaag gttaaaaaag tcatgccagc tgccttcgcg gaacttcaag
    12961 gagattacct gtatatgctg accactccga gtcgacaaga aatggagcag atgattgcag
    13021 atttcggaca acgtcaagaa taagaactat cggattaagg aaattaactc ccttaatcct
    13081 tttgttttgt ctgataaaaa ataagagcat cccaacaagg tagctatcat aaaacttgtt
    13141 cgataactat ttgaagcagg attaggtggt cagaaattaa attttaatat ttcaattgag
    13201 tcatagtatt gtgtttgcgt atccttaaat cagctaaaag gatccatgac gacacctata
    13261 cgatatagtt ttcaagatac caaacaagtc tattaatatt caatgaaaat caaagagcaa
    13321 actaggaagc tagccgcagg tttctcaaaa cactgttttg aggttgtgga tagaactgac
    13381 agagtcagta tcatatacct acggcaaggt gaagctgacg tggtttgaag agattttcga
    13441 agagtataaa atattcaggt gacgcataga tatagttaat tgaagctttg tttgaaatct
    13501 gataaaataa tgatattact aagttttaaa aactaaagaa aagggaagat atgattacag
    13561 gtgaattaaa aaataaaatc gatcagctgt gggaaattct ttggacagaa ggaaacgcaa
    13621 atcctttaac aaatattgaa cagttgactt atctcttatt tatgaaagat ttggatagtg
    13681 tcgagcttgg acgtgaaagt gatgctgaat ttctagggat tccttatgag ggagtttttc
    13741 caaaagataa acctgaatac cgttggtcaa cttttaaaaa tataggagat gctcaggaag
    13801 tttatcgttt aatgactcag gagatttttc cgtttattaa aaatctcaag ggggatacag
    13861 atgatacagc cttttcacga tatatgcgag aagctatttt tcaaataaat aaacctgcta
    13921 cgcttcaaaa ggcaatttct atcttagatg tttttccaac taggggatta gatgtagatt
    13981 ttgataatga caaacaaagt attactgata tcggagatat ctatgaatat ctgttatcaa
    14041 aattgtcgac cgcaggtaaa aatggacagt tccgtacatc tcgtcacatc atcgatatga
    14101 tggttgagtt gatgcaaccg actatcaaag atatcatctc agatcccgct atgggttctg
    14161 ctggcttctt agtatctgct agccgttact taaagcgtaa gaaagatgaa tgggaaacca
    14221 atacagataa tatcaatcat tttcataatc agatgtttca tggaaatgat acggatacga
    14281 ctatgttgag acttggggcg atgaacatga tgctacatgg agtagaaaat ccacaaatca
    14341 gttaccttga ctcgctgtct caagataatg aagaagccga taaatatact ttggttttag
    14401 caaatcctcc ttttaagggc tcacttgact acaattcaac ctctaatgac cttcttgcaa
    14461 ccgtaaaaac caaaaaaaca gaattactct ttctttctct tttcttgcga actttaaaac
    14521 caggtggacg agcagcagtt atcgtacctg atggtgtcct ttttggttcg tctaaagctc
    14581 ataaaggaat tcgtcaggaa attgtagaga atcataagct tgatgctgta atctcaatgc
    14641 ctagtggtgt gttcaagcct tatgctggag tttcaactgc cattctcatc tttacaaaaa
    14701 ctggtaatgg tggtactgac aaagtctggt tttacgatat gaaagcggat ggtttaagtt
    14761 tggatgataa gcgacaaccg attagcgaca atgatattcc agatattatc gaacgctttc
    14821 atcatcttga aaaagaagca gaacgtcaga gaacggatca atctttcttt gttccagttg
    14881 ctgagataaa ggaaaatgat tatgatttgt ctatcaataa atataaagag attgagtatg
    14941 aaaaagttga gtatgaacca acagaggtca tcttaaagaa aatcaatgat ttagaaaaag
    15001 aaattcaagc tggcttggct gaattagaaa aattactcaa gtagagaggt ggctgtatga
    15061 aaaaagtgaa gttgggggaa gtcttatctc taaaaaaagg caagaaagcc actgtacttg
    15121 ctgaacaaac aactctaagc caacgttata ttcaaataga tgatttaaga aataataata
    15181 atttaaaatt cactgaaagt ttaaatatga ctgaagcact cccagatgat attctgatag
    15241 catgggatgg agctaatgca ggaacagttg gttatggatt atcgggagct gttggtagta
    15301 caattacggt cttaaaaaag aatgagcgat acaaagaaaa aattatatca gattacttgg
    15361 gagtcttttt ggaaagtaaa tcgcagtatt tacgagaaca ttcaacaggt gcaacaattc
    15421 ctcatttaaa caagaatata ttacttgatt tacaattaga attgctaggt atcgaagaac
    15481 aagagaacat tatctgtatt cttaatacga ttaaaaggct tattactaaa agaaaattgc
    15541 agttagatga actaaacttg ctcgtcaaat cccgatttaa cgagatgttt ggggatcctt
    15601 taaataataa caagaaattt gcagtaaaaa cgggacaaca gtgttttaaa ttttcaagtg
    15661 gaaaatttct tgataaacat gatagagttt ttgaaggtta tcctgcctat ggtggaaatg
    15721 gcattgcttg gaaaagtcga aaatatttaa ttgataatcc tactatcatc attggtcgtg
    15781 taggtgcata ttgtggtaat gtaagaacta cccacggtaa ggtatggata agtgacaatg
    15841 ctatatatat aaaagaatt
//
LOCUS       DAVKZB010000038        15439 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKZB010000038 DAVKZB010000000
VERSION     DAVKZB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.038"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            89..1114
                     /gene="trpS"
                     /locus_tag="VXA80_001056"
     CDS             89..1114
                     /gene="trpS"
                     /locus_tag="VXA80_001056"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV0353565.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1266..2744
                     /gene="guaB"
                     /locus_tag="VXA80_001057"
     CDS             1266..2744
                     /gene="guaB"
                     /locus_tag="VXA80_001057"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV0353566.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSINEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2878..3351)
                     /gene="tnpA"
                     /locus_tag="VXA80_001058"
     CDS             complement(2878..3351)
                     /gene="tnpA"
                     /locus_tag="VXA80_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEV0353567.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            complement(3531..4628)
                     /gene="recF"
                     /locus_tag="VXA80_001059"
     CDS             complement(3531..4628)
                     /gene="recF"
                     /locus_tag="VXA80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV0353568.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(4631..4999)
                     /gene="yaaA"
                     /locus_tag="VXA80_001060"
     CDS             complement(4631..4999)
                     /gene="yaaA"
                     /locus_tag="VXA80_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV0353569.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEPDEYQADKVEKEQIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            5158..6408
                     /locus_tag="VXA80_001061"
     CDS             5158..6408
                     /locus_tag="VXA80_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0353570.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGYMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            6405..7688
                     /locus_tag="VXA80_001062"
     CDS             6405..7688
                     /locus_tag="VXA80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0353571.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7730..8551
                     /locus_tag="VXA80_001063"
     CDS             7730..8551
                     /locus_tag="VXA80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0353572.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVTLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            8562..9107
                     /gene="pgsA"
                     /locus_tag="VXA80_001064"
     CDS             8562..9107
                     /gene="pgsA"
                     /locus_tag="VXA80_001064"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV0353573.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            9104..9931
                     /locus_tag="VXA80_001065"
     CDS             9104..9931
                     /locus_tag="VXA80_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0353574.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9916..10755
                     /locus_tag="VXA80_001066"
     CDS             9916..10755
                     /locus_tag="VXA80_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0353575.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10748..11542
                     /locus_tag="VXA80_001067"
     CDS             10748..11542
                     /locus_tag="VXA80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0353576.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            11601..12419
                     /gene="mreC"
                     /locus_tag="VXA80_001068"
     CDS             11601..12419
                     /gene="mreC"
                     /locus_tag="VXA80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV0353577.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTVVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            12419..12913
                     /gene="mreD"
                     /locus_tag="VXA80_001069"
     CDS             12419..12913
                     /gene="mreD"
                     /locus_tag="VXA80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV0353578.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            13007..14185
                     /locus_tag="VXA80_001070"
     CDS             13007..14185
                     /locus_tag="VXA80_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV0353579.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            complement(14284..>15439)
                     /locus_tag="VXA80_001071"
     CDS             complement(14284..>15439)
                     /locus_tag="VXA80_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0353580.1"
                     /translation="KLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKR
                     RFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIR
                     KLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHF
                     LKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIM
                     NQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQDLK
                     HHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         4756 a         2439 c         3266 g         4978 t
ORIGIN      
        1 aaaagcaagg ctgatttatt ttgataaatt acggttattt cattaaaaaa atgctataat
       61 tgaaaggact atatcgaagg agaacaaaat gactaaaccc attattttaa caggagaccg
      121 tccaacagga aaattgcata ttggacatta tgttggaagt ctcaaaaatc gagtattatt
      181 acaggaagag gataagtatg atatgtttgt gttcttggct gaccaacaag ccttgacaga
      241 tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga aatgtggctt tggattatct
      301 tgcagttgga ttggatccaa ataagtcaac tatttttatt caaagccaga ttccagagtt
      361 ggctgagttg tctatgtatt atatgaatct agtttcgtta gcacgtttgg agcgaaatcc
      421 aacagtcaag acagagattt ctcagaaagg atttggagaa agcattccga caggattctt
      481 ggtctatcca atcgctcaag cagctgatat cacagctttc aaggctaatt atgttcctgt
      541 tgggacagat cagaaaccaa tgattgagca aactcgtgaa attgttcgtt cttttaacaa
      601 tgcatataac tgtgatgtct tggtagagcc ggaaggtatt tatccagaaa atgagagagc
      661 agggcgtttg cctggtttag atggaaatgc taaaatgtct aaatcactaa ataatggtat
      721 ttatttagct gatgatgcgg atactttgcg taaaaaagta atgagtatgt atacagatcc
      781 agatcatatc cgcgttgagg atccaggtaa gattgaggga aatatggttt tccattatct
      841 agatgttttt ggtcgtccag aagatgctca agaaattgct gatatgaaag aacgttatca
      901 acgaggtggt cttggtgatg tgaagaccaa gcgttatcta cttgaaatat tagaacgtga
      961 actaggtcct attcgtgagc gccgtattga atttgctaag gatatgggag aagtttataa
     1021 tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg ggtcaaaccc tatctgaggt
     1081 aaaaggggca atgggacttc attactttaa ctaagtttat tttacaagaa actatcattt
     1141 ctatctcgaa gaaaagatag ttttttattt acccctgtaa catttgacaa atttttatag
     1201 aatggtatga tagatacaat ataaaaagag tcaagctcaa aaagaaagaa aagaggaaac
     1261 ttcgaatgtc taattgggac actaaatttt tgaaaaaagg ttttaccttt gatgatgtat
     1321 tgcttattcc agctgaaagt catgtgttgc ctaacgatgc agatttaaca actaaattgg
     1381 cagataattt gactttaaat atcccaatta ttaccgctgc catggacaca gttacagaga
     1441 gtcaaatggc cattgctatt gctcgtgcag gtggtctcgg agttatccat aaaaacatgt
     1501 caattgctca acaagcagac gaggttcgta aggtaaaacg ttctgaaaat ggagttatta
     1561 ttgatccgtt cttcttgacg cctgaacata caattgctga agcagatgag cttatgggtc
     1621 gttaccgcat cagtggtgtt ccagttgttg aaacacttga aaatcgtaaa ttggttggta
     1681 ttttgacaaa ccgagatctt cgttttattt cagattataa tcaaccaatt tcaaaccata
     1741 tgactagtga aaatcttgtt actgctcctg tgggtacgga tcttgcaacg gctgagagta
     1801 ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga tgaagaaggc agtctttctg
     1861 gtttgatcac tatcaaagat attgaaaaag ttattgagtt tccaaatgcg gctaaagatg
     1921 aatttggtcg tcttctagtt gcaggtgcag taggtgttac ttcagataca tttgaacgtg
     1981 cagaggctct ttttgaggca ggagcggatg cgattgttat tgatactgca catggtcatt
     2041 ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca tttcccagat cggactttga
     2101 ttgctggaaa tattgctact gctgaaggtg cacgtgccct ttatgaagcg ggtgtagacg
     2161 ttgttaaggt tggtattgga ccaggttcta tctgtactac tcgtgtgatt gctggtgttg
     2221 gtgttccgca agtaacagct atctacgatg ctgcagctgt tgcgcgcgaa tatggtaaaa
     2281 cgattattgc tgacggtggg atcaagtatt ctggagatat tgtaaaagca cttgctgcag
     2341 gtggaaatgc tgttatgctt ggatctatgt ttgctggaac tgatgaagct ccaggcgaaa
     2401 ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg tatgggatca attgctgcta
     2461 tgaagaaagg ttcaagcgac cgttatttcc aaggttctat caatgaagca aacaagctcg
     2521 ttccagaagg aattgaaggt cgtgttgctt ataaaggagc ggcagctgat attgttttcc
     2581 aaatgattgg tggtattcgc tctggtatgg gttactgtgg tgcagctaac cttaaagaac
     2641 tacacgataa tgctcaattt attgaaatgt ctggtgctgg tttgaaagaa agccatcctc
     2701 atgatgtgca aattactaat gaggcaccaa attattctat gtaaaaaatc aaaaccacca
     2761 gtgtgaactg gtggtttgtt ctgcggctat aagccgcttc taccggccag ggccaaaggc
     2821 ccaccgaaat agcttcctcg cgcaccactt tcccgagcag gtgctaaagc accttagtta
     2881 cttcctctta tttatttcgc cagtaaacgg atctactgac tcgaataacg tgagctggtc
     2941 tgctactctg tcttcttgta attgattctg aatatattca gctatcactt tctgattacg
     3001 gcctaccgta tctacataat agcctctaca ccaaaacttg cgattgccat atttgtattt
     3061 taaattcgca tgcttatcaa aaatcatcaa actgctcttg ccctttaaat agcccataaa
     3121 ggacgaaaca ctaagtttcg gaggaatact gataagcatg tgaatatggt ctgaacaagc
     3181 attcgcttca tggattatta cacccttacg ctcacataag tcacgtatga ttcttccgat
     3241 actagctttg tatctgccat aaatgatttg acgacgatat ttgggtgcaa aaacaatatg
     3301 atatttacaa ttccatgtgg tatgtgataa actttgatta tcctctctca tgaggtacct
     3361 cctgtatgat atgttgtagt ggcggagaaa ccacttctat cttatcattt taggaggttc
     3421 tttttgttac cacgctaaaa gctctatgga accactagca tagctagtgg ttttcgggag
     3481 acaacaatga aaagaactcc agtgaaaaca ggagttcttt tacaatgttg tcaatttcca
     3541 tttacagcag ctttaccatc ctgaatagtg aagatactta gattttctgg tagattttga
     3601 agatggtcta agcttgttgt tgtgataaag gtttggattg attgagaaat cgtttctaat
     3661 aattttaatt gtctagtgtt gtcaagttca ctcatcacat cgtcaagcag taatatagga
     3721 gattctgtgg taatgctttc cattaattcg atttctgcta attttatcga gaggacgaga
     3781 ctacgatgtt gaccttggct tccgaaacta gcatccatcc catttatata aaaagaaatg
     3841 tcatctcgat gaggaccgac accagtattc tttttaaata aatctctgga tttacttttt
     3901 tctaaagcaa ttttgaaaga ttcggataag ttttgtttgt cagttatatt gacagaagat
     3961 tgataggata ttgacaactc ttcgatctga ttagagagtt caaaatgttt cttacgccca
     4021 aatgattcta gtttttttat gaaatctaag cggtgattca ttacacgaca tccataatca
     4081 actagctgat catctaacac agaaaggaat gtttcatcta ttttttgatc tgattttagg
     4141 taagtgtttc tttgctttag gatgtggtta taattggtta agtcagataa gtagattggc
     4201 ttaatttgcc caagttccat atcaatgaat tttcgtcgaa tcgaaggtgc tcctttaatt
     4261 agttgtaaat cttcaggagc aaataagaca acattcatgt gtcctacata atctgaaagg
     4321 cgtgcctgtt ttaagtgatt aacttttgtt acacggcctt tttgtgttag ttcgatttct
     4381 aggggaatgg atccagtttt tttctgaacg agacctgaaa gatgaagttg ttcctcatca
     4441 aaatgaatga gatttttatc tgttcgagtt cgatgactac gcgttaaggc taaaaaatag
     4501 atagcctcta acatgtttgt ttttccttgt gcattacgtc ccaaaaagac attcaattta
     4561 ggattaaagt ctattttcgt ttctttgtaa ttacgaaaag tcttgagaga tagatgttgt
     4621 agccacatga ttatctacca gggaatcgtg gagcttgttg gcttttaggt gatgaagcag
     4681 gtttcgattt gtctttttta actcccttat tcatcttttt gacaagttta gcgatctgtt
     4741 ctttttcaac tttatcagct tggtattcat ctggttcttc agaagtaggt tgtgtcaaca
     4801 agatgtcaat gttcatgtca gggatgtcaa ctttatcgcc aatacgaagt ttttttccac
     4861 gacgactctc taattcccta ttaaagtaaa cagaatgttc agagagaaat gatttaatag
     4921 ctcctccgct atgtgtaatt ccaagttctt tgagtagtgc ttggagggta ataaattctt
     4981 caaataattt gtattccata attcacctca atctttggta ttataccata ttttcctaaa
     5041 aatagtgaga gagacagcga gaaatgtaag cgatttttat ttcaaaagtg ttacagggaa
     5101 caagaaaatt tcaggttttc gtgatataat agaagtctgt atataaggag gtaaatcatg
     5161 gagttagtgc atggaatttc aacacatttt atccaatcaa aaaagtttaa aacaaacaaa
     5221 attaccgtgc gttttaccgc tccattatcc cttgatacga ttgcaggtta catgttgagt
     5281 gcaagtatgc tagagactgc taatcagatg taccccactt ctcaagattt gaggagacac
     5341 ttggccagtc tatacggtac agatatgtca accaattgtt tcagaagagg gcaaagccac
     5401 attatagaat tgacatttac ctatgttcgt gatgagtttt taagtaggaa aaacgtgcta
     5461 acctctcaga ttttggaact tgtaaaagaa actctttttt cacccgtagt agttgataat
     5521 gggtttgatc cggccttatt tgaaattgag aaaaaacaat tgctagcaag tttagcagct
     5581 gatatggatg attcttttta ttttgcacat aaagaattgg ataaattgtt ttttcatgat
     5641 gaacgtcttc aattggaata tagtgattta cgaaatcgta ttttagctga aactccacaa
     5701 agttcttatt cttgtttcca agaattttta gccaatgatc gaatagattt ctttttccta
     5761 ggtgatttta atgaggttga aattcaaaat gtattagaat catttggctt taaaggtcga
     5821 aaaggagatg tgaaggttca gtattgtcaa ccttattcta atatccttca ggaaggtatg
     5881 gttcggaaaa atgtgggaca atccattttg gaattaggtt atcattaccg ttctaaatat
     5941 ggtgatgagc aacatttacc catgattgta atgaatggtt tacttggtgg atttgctcac
     6001 tctaagctct ttacaaatgt ccgtgaaaat gctggattag cttataccat ttcaagtgag
     6061 cttgatttat ttagtggatt cttgaggatg tatgctggta tcaatcgaga aaatcgtaac
     6121 caggctcgta aaatgatgaa taatcaactg cttgatttaa aaaaaggtta ttttacagag
     6181 tttgagttaa atcagaccaa ggaaatgatt cgttggtcgt tgttactttc tcaagataat
     6241 caatcttcat tgattgaacg tgcttatcaa aatgccttat ttggaaaatc ttcagcagac
     6301 tttaaaagtt ggattgcaaa gcttgaacaa attgacaaag atgctatttg tagagtagct
     6361 aataatgtga aactacaagc gatttacttt atggaaggaa tagaatgaca aaggttgttt
     6421 ttgaagaaaa atactatcca gctgtaaaag aaaaggttta tcgaactcgt ttggccaacg
     6481 gattgacagt tgctcttttg cctaaaaagg aatttaaaga ggtttacggg agtgtaactg
     6541 tacagtttgg ttcggtagat acgtttgtca cagaagttga cgggtatgta aaacaatatc
     6601 ctggaggaat tgctcatttt cttgaacata aattatttga gagagaagat tctagtgatt
     6661 tgatgtcggc ttttacgagt ctaggtgcag atagtaatgc ctttacaagc tttacaaaaa
     6721 caaactatct tttttcagca acggattatt ttttagaaaa tttagattta cttgatgaat
     6781 tggtaacatc agcacacttt actgaagctt ccattctgac agagcaggat attattcagc
     6841 aagaacgaga aatgtaccaa gatgatccag attcgtgttt attcttttca actttagcga
     6901 atttgtatcc tggtacacct ttagcaactg atatagttgg aagtgaggag tccatttccc
     6961 aaatcaatct aactaatttg caagaaaatt ttacaaagtt ttacaaacct gtaaacatgt
     7021 ctctgttttt agttggtaat tttgatgtgg agcgagtaca ggactatttt gaaagcaaag
     7081 aactgaaaga ttcagatttt caggaagtag caagagaaaa gttgttttta cagcctgtaa
     7141 agccaacaga tagtatgaga atggaagtat cttctcccaa actagcgatt ggagttagag
     7201 gtaagcgaga agtttctgaa gcggattgct atcgacatca tattttatta aaattattgt
     7261 ttgcaatgat gtttggttgg acttcggatc gttttcaaaa atgttatgaa tcaggtaaaa
     7321 ttgatgcatc cttatctctg gaagttgaag taacaagtcg ctttcatttt gtcatgttga
     7381 caatggatac gaaagagcca gttgctttgt ctcatcaatt taggaaggct attcgtaatt
     7441 ttacaaagga tttagatatt acagaggaac atttagatat tatcaaaaga gagatgtttg
     7501 gcgaattttt cagtagcatg aactctcttg aatttattgc aacgcaatat gatgcttttg
     7561 aaaatggtga gacaattttt gatttgccga aaattttaca ggaaattact ttagaggatg
     7621 ttcttgatgc tggacatcat ttaatagatg atggtgacat agttgatttt acaatattcc
     7681 catcgtagta acctatcata atagacacta gaaagaaggg atgacaagta tgagaaaaaa
     7741 aacaattgga gaggttttac gattagctag aatcaatcag ggattgagtt tagatgaatt
     7801 gcagaaaaag acagagatcc agttagatat gttggaagca atggaagcag acgatttcga
     7861 tcaacttcca agtccttttt acacgcgttc tttcttgaaa aaatatgcat gggctgttga
     7921 gttagatgac caaattgttt tggatgctta tgattctggg agtatgatta cttatgagga
     7981 agtagatgtt gatgaagatg agttgacagg tcgtagacgt tcaagtaaga aaaagaagaa
     8041 aaaaacatca tttttacctt tattttattt tatccttttt gctttatcga ttttaatttt
     8101 tgtgacttat tatgtttgga actatattca aactcaacca gaggagcctt ctctttctaa
     8161 ttacagtgtg gttcaatcaa caagttcaac tagctctgtt ccccactcct caagtagtag
     8221 ttcttctagt atagaatcag ctataagtgt atcaggcgaa ggaaatcatg tagaaatcgc
     8281 ttataagaca agtaaggaaa cagttaaatt gcaattggca gtttcagatg ttacaagttg
     8341 ggtcagtgtt tcagaaagcg aacttgaggg cggtgtaacc ttatcgccaa agaagaaaag
     8401 tgcagaagca acagttgcaa ctaaaagtcc tgtaacaatt acgttaggtg ttgtaaaagg
     8461 tgttactttg acagtagata atcagactgt tgatttatcg aaattaacag ctcagactgg
     8521 acaaatcact gtaaccttta ctaaaaatta aggaaaaacg aatgaaaaaa gaacaaattc
     8581 ccaatctctt aacaataggt cgaattctct ttatacctat ttttatcttt attttaacga
     8641 taggaaattc gatagagagt catatagttg cagctattat ctttgctgtt gccagtatta
     8701 ccgactattt agatggatat ttagctcgta aatggaatgt ggtcagtaat tttggtaaat
     8761 ttgcagatcc tatggcggat aagttactag ttatgtcggc ttttattatg ttgattgagt
     8821 taggtatggc tccggcttgg attgttgcag tgattatctg tcgtgagtta gctgtgacag
     8881 gtttaaggct tttattggtt gaaactggtg gaacaatttt agcagcagca atgcctggaa
     8941 aaattaaaac ttttagtcag atgtttgcta ttattttctt gctattacat tggactttgc
     9001 ttggtcaagt tctactttat gtagccttat ttttcactat ctactctggc tatgactatt
     9061 tcaagggtag tgcctatgta tttaaaggga catttggttc gaaatgaaat caataattga
     9121 tgtaaaaaat ctttcttttc gctataaaga aaatcagaac tactacgatg tgaaggatat
     9181 tacgtttcac gtgaaacgtg gagaatggct ttcgattgta gggcataatg gtagtggtaa
     9241 atcaacgacg gttcgattaa ttgatggctt actggaagca gaatccggag agattgtaat
     9301 tgatggccaa cgtttgactg aggaaaatgt ttggaatata cgtcgtcaaa tcggtatggt
     9361 ttttcaaaat ccagacaatc aatttgttgg agcgactgtt gaagatgatg ttgcttttgg
     9421 tttggaaaat cagggacttt ctcgtcaaga aatgaaaaag agagtagaag aagctctggc
     9481 tttagttggc atgttggact ttaaaaagag agagccagcg cgtctatcag gtggccaaaa
     9541 gcaacgtgtg gccattgcag gtgttgtagc cctaagacca gctattttaa tcttagatga
     9601 agcaacgagt atgttggatc ctgaggggcg tagagaactt attgggacag taaaaggaat
     9661 tcgaaaagac tatgatatga cggtcatttc tattacccat gatttggaag aagtcgccat
     9721 gagtgatcgc gtattggtca tgaaaaaagg ggaaattgaa tcaactagta gtccaaggga
     9781 gcttttctct cgaaatgatt tagatcaaat tggattagac gatccttttg ctaatcaatt
     9841 aaaaaaatct ttgagccaga atggctatga tttacctgaa aattatttga cagaaagtga
     9901 gctagaggat aagctatggg aattgctcta gaaaatgtga attttacata tcaagaaggt
     9961 actcccttag cttcagcagc tttgtcggat gtttctttga cgattgaaga tggctcttat
    10021 acagctttaa ttgggcacac aggtagtggt aaatcaacta ttttacaact cttaaatggt
    10081 ttattggtgc caagtcaagg gagtgtgagg atttttgata ccttaatcac ctcgacttct
    10141 aaaaataaag atattcgtca aattagaaaa caggttggct tggtatttca gtttgctgaa
    10201 aatcagattt ttgaagaaac ggttttgaag gacgttgctt ttggaccgca aaattttgga
    10261 gtttctgaag aagatgctgt gaagactgcg cgtgagaaac tggctctggt tggaattgat
    10321 gaatcacttt ttgatcgtag tccgtttgag ctgtcagggg gacaaatgag acgtgttgcc
    10381 attgcaggca tacttgccat ggagccagct atattagtct tagatgagcc aacagctggt
    10441 ctagatcctc tagggagaaa agagttgatg accctgttca aaaaactcca ccagtcaggg
    10501 atgaccatcg tcttggtaac gcatttgatg gatgatgttg ctgaatatgc aaatcaagtc
    10561 tatgtaatgg aaaagggacg tttagtaaag gggggcaaac caagtgatgt ctttcaagac
    10621 gttgttttta tggaagaagt tcagttggga gtacctaaaa ttacggcctt ttgtaaacga
    10681 ttggctgata gaggcgtgtc atttaaacga ttaccgatta agatagagga gttcaaggag
    10741 tcgctaaatg gatagtatga ttttggggcg ttatatccca ggggattcga ttgttcaccg
    10801 attggatcca cgtagcaaat tgcttgctat gatgctactg attttgattg tgttttgggc
    10861 taataacccc ttgacgaatc tgattctttt tatagcgaca gggatattta ttgccttgtc
    10921 aggagtatct ctttcatttt ttattcaggg cttgaagtct atgtttttct tgattgcctt
    10981 cacaactatt tttcaactat ttttcatttc taatgggaat gttttatttg agttttcgtt
    11041 tgtgagaatc acggattatg ctttgcaaca agctgggatt attttttgtc gctttgtatt
    11101 gattattttc ttttcaactt tgttaacctt aacaaccatg cctttaagtt tggcatcagc
    11161 tgtcgaagct ttattagcac ctttaaagcg tgtgaaagtt ccagttcatg aaattggatt
    11221 gatgctgtct atgagtttgc gttttgtccc aaccttgatg gatgatacga ctcggattat
    11281 gaatgcacag aaagctcgtg gagtggattt tggagaagga agcatcgttc aaaaagtaaa
    11341 ggcgatgatt cccattttga ttcctctttt tgcgacaagt ttaaaacgtg cagattcctt
    11401 ggctatcgct atggaagcgc gtggctatca gggtggaaaa ggcagaagtc aataccgaca
    11461 attgaaatgg actctaaagg atacgctgac cattcttgtt attctcgtac ttggttgttg
    11521 tttatttttc ttaaaatctt agtagctttc aggaattgat aaaaagttac tgtaacagtt
    11581 tttgatataa aggtaggaat atgaaccgtt ttaaaaaatc aaaatatgtc attattgttt
    11641 ttgtcactgt tctgcttgtg tcagctctct tagcgacgac ttattcaagt acagttgtga
    11701 caaaattagg agatggaatc tcattggttg atagagttgt acaaaaacct tttcagtggt
    11761 ttgattctgt caaatcagat ttggctcatt tgacacgaac atataatgaa aatgaaagtt
    11821 tgaagaaaca gctttaccaa ttagaagtta aatcaaatga ggtggaaagt ttaaagacag
    11881 aaaatgaaca actgcgccaa ttgcttgata tgaagtctaa attgcaagcc acaaagactt
    11941 tagcagcaga tgttattatg cgttctccgg tatcttggaa gcaggagttg accttagatg
    12001 caggtagatc aaaaggtgct tctgagaaca tgttagctat tgcaaatggt ggcttgattg
    12061 ggagtgtttc aaaagtagag gagaactcta ctatagtcaa ccttctgaca aatacggaaa
    12121 atgctgataa gatttctgtt aaaattcaac atggctctac tacaatttat ggaattatta
    12181 ttggctatga caaggaaaat gacgttctta aaattagcca attaaatagt aatagcgata
    12241 ttagtgcggg agataaggtg actactggtg gattaggaaa ctttaacgtt gctgatattc
    12301 ctgttggtga agtggttgcc acaacgcata gtacagacta tttgacacga gaagtaactg
    12361 ttaaattgag tgcagatact cataatgtag atgtgataga attagtgggg aattcataat
    12421 gagacagttg aagcgagttg gagtattttt attgcttcct ttctttgttc taattgacgc
    12481 ccatattagc cagcttctgg gctcattttt cccccatgta catttggcta gtcattttct
    12541 ttttctattt ctcttatttg agacgataga agtatcagag tatctctacc tagtctattg
    12601 ttttgttata ggcttggttt atgatgttta ctttttccat ctaataggga ttacaactct
    12661 cttatttatc ttattgggag ccttccttca taaattgaat agtgttattt tgttgaatcg
    12721 ttggacaaga atgctagcta tgattgtgct gacattcctg tttgaaatgg gtagttatct
    12781 tttggctttt atggtagggt tgacagtaga tagcatgtcg atttttatag tctatagctt
    12841 ggtaccgacg atgattttaa attttttatg gattactgtt tttcaattta tttttgaaaa
    12901 atattatcta taagaacgac atataaatgt aacaaaggcg taatatttat taggcctttt
    12961 tttggtatac tagtattgtc tttaaaagaa ggagtatcta cgtaatatga agaaaaaaat
    13021 cttagcgtca cttttattaa gtacagtaat ggtttctcaa gtagctgttt taacaactgc
    13081 gcatgcagaa acgactgatg acaaaattgc tgctcaagat aataaaatta gtaacttaac
    13141 agcacaacaa caagaagccc aaaaacaagt tgaccaaatt caggagcaag tatcagctat
    13201 tcaagctgag cagtctaact tgcaagctga aaatgataga ttacaagcag aatctaagaa
    13261 actcgagggt gagattacag aactttctaa aaacattgtt tctcgtaacc aatcgttgga
    13321 aaaacaagct cgtagtgctc aaacaaatgg agccgtaact agctatatca ataccattgt
    13381 aaactcaaaa tcaattacag aagctatttc acgtgttgct gcaatgagtg aaatcgtatc
    13441 tgcaaacaac aaaatgttag aacaacaaaa ggcagataaa aaagctattt ctgaaaaaca
    13501 agtagcaaat aatgatgcta tcaatactgt aattgctaat caacaaaaat tggctgatga
    13561 tgctcaagca ttgactacga aacaggcaga actaaaagct gctgaattaa gtcttgctgc
    13621 tgagaaagcg acagctgaag gggaaaaagc aagtctatta gagcaaaaag cagcagctga
    13681 ggcagaggct cgtgcagctg cggtagcaga agcagcttat aaagaaaaac gagctagcca
    13741 acaacaatca gtacttgctt cagcaaacac taacttaaca gctcaagtgc aagcagtatc
    13801 tgaatctgca gcagcacctg tccgtgcaaa agttcgtcca acatacagta caaacgcttc
    13861 aagttatcca attggagaat gtacatgggg agtaaaaaca ttggcacctt gggctggaga
    13921 ctactggggt aatggagcac agtgggctac aagtgcagca gcagcaggtt tccgtacagg
    13981 ttcaacacct caagttggag caattgcatg ttggaatgat ggtggatatg gtcacgtagc
    14041 ggttgttaca gctgttgaat caacaacacg tatccaagta tcagaatcaa attatgcagg
    14101 taatcgtaca attggaaatc accgtggatg gttcaatcca acaacaactt ctgaaggttt
    14161 tgttacatat atttatgcag attaatttac agagggactc gaatagagcc ctcttttcag
    14221 gttttaccgt gacaatccct attaaaaatg actctttgtc aactgtagtg ggttgaaaaa
    14281 aagctaagct cgagaaagga caaatttcgt cctttctttt ttgatattca gggcgataaa
    14341 aatccgtttt ttgaagtttt caaagttccg aaaaccaaag gcattgcgct tgataagttt
    14401 gatgagatta ttggtcgctt ccaatttggc gttagaatag tgtagttgaa gggcgttgac
    14461 gattttctct ttgtccttta gaaaggtttt aaagacagtc tgaaaaagag gatgaacctg
    14521 ctttagattg tcctcaatga gtccgaaaaa tttctccggt tccttattct gaaagtgaaa
    14581 cagcaagagt tgatagagct gatagtgatg tttcaagtct tgtgaatagc tcaaaagctt
    14641 gtttaaaatc tctttattgg ttaaatgcat acgaaaagta gggcgataaa aatgtttatc
    14701 gctgagttta cgactatcct gttgtatgag cttccagtag cgcttgatag ccttgtattc
    14761 atgagacttt cgatccaatt gattcatgat ttgaacacgc acacgactca tagcacggct
    14821 aagatgttgt acaatgtgaa agcgatccaa cacgatttta gcacatggga aaagttgtct
    14881 agctaagtca tagtaaggac taaacatatc catagtaata attttgacgc gacatcggac
    14941 ggctctatca tatttaagaa agtgatttcg aatgatagct tgtgttcttc cctcaagaac
    15001 agtgatgata ttgagattgt taaaatcttg cgcaatgaag ctcatctttc cctttgtaaa
    15061 agcatactca tcccaagaca taatctcagg aagacaagaa aaatcatgtt taaagtgaaa
    15121 atcattgagc ttacgaataa cagttgaagt tgagatggaa agctgatggg caatatcagt
    15181 catagaaatc ttttcaatta acttttgagc aatcttttgg ttgatgatac gagggatttg
    15241 gtgattcttc ttgacgatag aagtttcagc gaccatcatt tttgaacagt gatagcactt
    15301 gaatcgacgc tttctaagga gaattctagt aggcatacca gtcgtttcaa gataaggaat
    15361 tttagaaggt ttttgaaagt catatttctt caattggttt ccgcactcag ggcaagatgg
    15421 ggcgtcgtag tccagtttg
//
LOCUS       DAVKZB010000039        15134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKZB010000039 DAVKZB010000000
VERSION     DAVKZB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.039"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..234)
                     /locus_tag="VXA80_001072"
     CDS             complement(<1..234)
                     /locus_tag="VXA80_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353581.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHR"
     gene            complement(242..721)
                     /locus_tag="VXA80_001073"
                     /pseudo
     CDS             complement(242..721)
                     /locus_tag="VXA80_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            complement(732..1430)
                     /locus_tag="VXA80_001074"
     CDS             complement(732..1430)
                     /locus_tag="VXA80_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV0353582.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGFQVYGVDQGENPLLEGDF
                     HFLQRDLTLDLEPIFGWCPQVDVLCNTAGVLDAYKPLLEQTAQEIQEIFEINYMTPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(1423..1659)
                     /locus_tag="VXA80_001075"
     CDS             complement(1423..1659)
                     /locus_tag="VXA80_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV0353583.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(1759..2685)
                     /locus_tag="VXA80_001076"
     CDS             complement(1759..2685)
                     /locus_tag="VXA80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV0353584.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVDELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(2760..3575)
                     /locus_tag="VXA80_001077"
     CDS             complement(2760..3575)
                     /locus_tag="VXA80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353585.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(3579..3899)
                     /locus_tag="VXA80_001078"
     CDS             complement(3579..3899)
                     /locus_tag="VXA80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0353586.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(4106..4186)
                     /locus_tag="VXA80_001079"
                     /pseudo
     CDS             complement(4106..4186)
                     /locus_tag="VXA80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4230..6227)
                     /gene="metG"
                     /locus_tag="VXA80_001080"
     CDS             complement(4230..6227)
                     /gene="metG"
                     /locus_tag="VXA80_001080"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV0353587.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(6351..7610)
                     /locus_tag="VXA80_001081"
     CDS             complement(6351..7610)
                     /locus_tag="VXA80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353588.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(7612..8313)
                     /locus_tag="VXA80_001082"
     CDS             complement(7612..8313)
                     /locus_tag="VXA80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353589.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(8297..9496)
                     /locus_tag="VXA80_001083"
     CDS             complement(8297..9496)
                     /locus_tag="VXA80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV0353590.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            9782..11128
                     /gene="gor"
                     /locus_tag="VXA80_001084"
     CDS             9782..11128
                     /gene="gor"
                     /locus_tag="VXA80_001084"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV0353591.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RINLPLHTHKVPAKLEKTAKGITIHFEDGTSHTASQVIWATGRRPNVKSLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            11325..11861
                     /locus_tag="VXA80_001085"
     CDS             11325..11861
                     /locus_tag="VXA80_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV0353592.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            11912..11998
                     /locus_tag="VXA80_001086"
                     /pseudo
     CDS             11912..11998
                     /locus_tag="VXA80_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(12067..12282)
                     /locus_tag="VXA80_001087"
     CDS             complement(12067..12282)
                     /locus_tag="VXA80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV0353593.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            12380..12700
                     /locus_tag="VXA80_001088"
     CDS             12380..12700
                     /locus_tag="VXA80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353594.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(12731..13069)
                     /locus_tag="VXA80_001089"
     CDS             complement(12731..13069)
                     /locus_tag="VXA80_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353595.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(13081..13800)
                     /gene="trmD"
                     /locus_tag="VXA80_001090"
     CDS             complement(13081..13800)
                     /gene="trmD"
                     /locus_tag="VXA80_001090"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV0353596.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(13790..14308)
                     /gene="rimM"
                     /locus_tag="VXA80_001091"
     CDS             complement(13790..14308)
                     /gene="rimM"
                     /locus_tag="VXA80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV0353597.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(14339..15127)
                     /locus_tag="VXA80_001092"
     CDS             complement(14339..15127)
                     /locus_tag="VXA80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0353598.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
BASE COUNT         4430 a         3398 c         2636 g         4670 t
ORIGIN      
        1 tcgatgcaga aaatcttgcc aattagtatt ttctgaaata ataatcttat aatctaaacc
       61 atattttcta acaaaatctt cccaaaatct ggaatccaac tcccctgcta ggtatagaaa
      121 attcccaaca tcataaaagc ttgttgtcct atcattgttt cggtagacag ttccaatttg
      181 agatttaaga ccatagagaa ccatattttt cttccatgtt ttaaatagtt ccattatccc
      241 tctacttcac cttccaattc atgtaagaag tcttgtccag ttctggaaat cctaataatt
      301 cagacttaac cttcaagact aatggcgatg cattttcttc tgtaatctct tgaatatcca
      361 tccaaatata tccaagtgaa tcattcgcac catcagacac agcttccgaa gtcgtaactt
      421 gaggtgcact ctcattcatt tcaacatcat acaaggccat gacatggtga accataaaat
      481 ttgttaactc ttctctgacg aaaacatcgt agattcaagg attagagtag cttctaacag
      541 taaatcccgt ctcttccata acttctctag tcagcgtttc cgtcagtcct tcaccaagtt
      601 gctgactgcc tctaggtaga tcataccgat gttgataagg gcctctcgtt ttttcaatgc
      661 agagtaactt cccattttct aagcaaacag cgtagactcc aaagtgtttt ttgatttcca
      721 tccaactcct cctacttcaa agaccagcca ccatctattg tcaagatttg tccttgcatg
      781 gcgctcgctt ttccacttgc taaaaaaaga ctaagctctg ctatttcctc tggctcaatc
      841 cagcgcttga ttggggtttc actagccacc cagtcagcca aaccacctgg ttcaaaatcc
      901 gcagcggtca tagctgtctt gactgctcct ggagcgatac caaagacctg aatcccagct
      961 tcagcatagt ctagagccaa ctgcttggtg aagccagcca aggcatgctt ggatgaagta
     1021 taggcgtgac cacctccacc tgctaggcta gaagcaatgg aacacatatt gatgatgatt
     1081 ccctttttat tttccagcat ttgtgtcaaa taataccgag tcaactcaac aggagtcatg
     1141 tagttaattt caaaaatctc ttgaatttcc tgcgcagtct gttccaacag tggtttgtaa
     1201 gcatccaaaa ctccggcagt attacacaaa acatccacct gagggcacca gccaaaaata
     1261 ggttccaagt ccaaggtcaa atctctctgt aaaaaatgga aatcaccctc taagagtgga
     1321 ttttcacctt ggtcaactcc ataaacttga aagcccttct ctaaaaagag tcgagcctga
     1381 gccaatccga tacctgaact cactcctgta ataagtacac gtttagtcat gcacttctac
     1441 ccaatccgtt gccaaaacat cacaagctgt cgggctccac atggaaaaac cttctccttc
     1501 gccagaaacg ttgattagga aataaggtgt cacttcaagt gcaagcccgt tttgttcaat
     1561 ggtgtcaaaa agttggacat agttttccgc acctccccaa ccagttcgta catatttttt
     1621 cttagccttt aagccaggta gaatttcttc aaatgtcatg tttttctcct ttaatgtcaa
     1681 taattctccc cttcattata acaaaaaaac cgctttaaaa cggctttttg actgtgattt
     1741 atttacatct tcttctactt acggcaaatt attccctgca gcaagataaa tttcatacca
     1801 ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt gcttaggatt
     1861 tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag aaatggcaat
     1921 agttgaagag gttactccat atttaatagc taaacgatca agtacttgat ttaaagcttg
     1981 aaatttctca tttccaacaa agttcccttt aaaatacccg aattgcaaga cagaccatgc
     2041 ttgaatgacc acatcgtgta atttgcaata ttcaaaaatg ctgccatctc gcatagctgc
     2101 ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa aagccgcact
     2161 caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca actccatcat
     2221 cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat aaaggagatt
     2281 aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa ggagcaagct
     2341 atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta taatatagtc
     2401 cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg actgaatcca
     2461 catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt cttcacaacg
     2521 accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa gtgctgtttc
     2581 tacaagctca tcaacttctt ttacagactt atcttttatt ctcatcattc cgagaacaat
     2641 ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat ttttctcctt
     2701 taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg gctttttgac
     2761 tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat tccaataaag
     2821 aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat ccactcataa
     2881 taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa tggacgaaag
     2941 accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac agacaaacta
     3001 tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat tcctacatat
     3061 cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat tttcatttta
     3121 ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa tccaatcaga
     3181 aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg acctttagaa
     3241 aaatcactta gtagttgata ataacgtaat accgccagga caagaattgg cgtcaaaaga
     3301 gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat ttttttagga
     3361 ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga gaaatctagc
     3421 atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc agcaagatta
     3481 aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc atgtagcaaa
     3541 gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt cttaatccct
     3601 tctacttttt gactcaaatc gtcccattgt tgccaaaaga ctgagacacg ctcttctcct
     3661 tctttcatta atgaaaaata cttccgatct ggaccatctg gcgacgggcg catgtcgcct
     3721 cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc tggaacgata
     3781 gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg ctctttttga
     3841 ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt ttctttcatc
     3901 acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat agtatatcac
     3961 ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct tcaaaccacg
     4021 tcagcgtcgc cttaccgtat gtagggttac tgacttcgtc agtttcatct acaacctcaa
     4081 aactgtgttt tgagctgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc
     4141 aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa taaaaaaaca
     4201 gaactagtct gaactagtcc tgtctacttt tacccaatca cacttccatt tggtacagct
     4261 ggatcaactg tgagaagggt taatttgcca tcatgttcag ctgagagaat cataccctgg
     4321 ctgacatatt ttttcatcat tttacgtggt ttgaggttag caacgatttg aactttcttg
     4381 ccgaccaatt cttgttcatt tggatagtat tttgcaattc ctgaaagaat ctgacgatct
     4441 tctccatcac cagcatccaa gcggaattga agcaacttat ctgaaccttc tactttagac
     4501 acttctttga cttctgcgac acggatttca accttgtcaa agtcttcaaa cttgatttca
     4561 tccttgttta gtttgagctc aacttcgtcc ggattccatt ctttttcgac tgctggttta
     4621 ttgccttcca tttgttcctt gatataggcg atttcttctt ccatatttag acgtggaaag
     4681 ataggtgttc ctttggcaac tacagtcaca tctgctggga agtcagccaa actcaagttt
     4741 tcaagactag aaacttcttc caaaccaagt tgagtcaaaa ctgcacgact agtttccatc
     4801 ataaatggtt caatcaagtg agcaactaca cgaatgctgg ctgccaagtg gctcatgaca
     4861 cttgccaatt ggtcacgaag agcttcatcc ttggccaaga cccatggtgc ggtctcatcg
     4921 atgtatttat tggtacgaga gatcagagtc cagactgctt caagcgcacg tggatagtca
     4981 actgcttcca tgtgtgtatg gaagtctgcg attgattgtt ctgcaacctc agcaagaaca
     5041 tgatcaaatt cagtcacacc ttctacatag gcagggattt gtccatcaaa gtacttatta
     5101 atcatggaaa ccgtacggtt aaggaggttc ccaaggtcat tagccaattc atagttgata
     5161 cgaccaacat agtcttcagg agtaaaggtt ccgtctgaac caactggaag gttacgcatg
     5221 aggtagtaac gaagtggatc tagtccataa cgctctacca acatttcagg gtaaacgaca
     5281 ttcccttttg acttagacat ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa
     5341 cgatcaggta atttaacatc caacatcata agaaggattg gccagtagat agagtggaag
     5401 cgaaggatgt cttttcctac catatggaag actgttccat tccagaactt gtcaaagtta
     5461 ccatgttcgt cttgagcgta gccaagagct gtcgcatagt taagaagggc atcaatccaa
     5521 acgtagacaa catgttttgg atttgatggg acaggcactc cccatgtaaa ggttgtacga
     5581 gataccgcca aatcttccaa acctggctcg atgaagttgc gtagcatttc attaaggcga
     5641 ccatctggcg tgataaattc aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat
     5701 ttgctaaggc gaaggaagta tgattcttca gaaacccatt caacctcatg acctgatgga
     5761 gcaataccac cagtcacatt tccagcttca tcacggaaaa cttctgccag ctggctttct
     5821 gtaaagaatt cttcgtctga tactgaatac caaccagagt attcacccaa gtagatatca
     5881 tcttgagcaa gtaagcgttc aaagacttgt gcgacaactt tttcatggta gtcatcagtt
     5941 gtacggataa atttatcgta tgagatatct agtaattgcc agagttcttt aactccaacc
     6001 gccattccat caacataggc ttgaggtgta ataccagctt cttccgcttt ctgctggatt
     6061 ttctgaccat gttcatcaag acctgtcaga taaaatacat cgtagcccat caggcgtttg
     6121 taacgtgcta ggacatcaca tgcgatagtt gtgtaggcag aaccgatatg aagtttccca
     6181 gatggatagt aaatcggcgt tgtaatataa aaattttttt cagacataat ttttcctttc
     6241 caggcaaatg aaacctgttt ttctaacact tcattatatc acatttttaa tgaatttcaa
     6301 tagggaaatc catacaaaaa caagatagac gagtgtccat cttgttgatc tcattcataa
     6361 cgaagggctt caattggatc aagtttcgat gccttgttgg ctggcaagac tccaaaaatc
     6421 ataccaacac tagccgaaac tgcaagacta aatagggcga ctgggattga tactccaact
     6481 tctatacctt ctattaaacc ttgcagtaac aaacctgcta aggcagttaa accacttgca
     6541 attgtcaagc caattaagcc acctaacaag gtcaaaatca tggattcaat caaaaactga
     6601 attaaaatat tggcacgtgt tgcacccaaa gccttacgaa gaccaatctc acgagtgcgc
     6661 tctgtcaccg aaaccagcat gatgttcatg acaccagttc ctccaacaaa gagagaaatc
     6721 cctgcgatgg aactaataat cgtcgtcata aaactaaacg attgttgaat ttctgcaaat
     6781 acaagggact catctgccac ctggtattct ccctgttgta agcctgcaag ctctgtcatt
     6841 tttcgtgcca gttctggacc cagagttggg gttaaactgg tatcattcac tcgaaagaca
     6901 atattagcta tttcatctac attaaaattc gcagcaaggg agatattggt agtaataggc
     6961 aagccaccaa acccatatat ttttgatctt ttagcctccg gactagtata aaccccaatg
     7021 acccggtaac taaatccatt gacttctaca accttgttaa tagcctcttg aggagattca
     7081 aataaactaa tggacaattc ctcatctagc aaaataacac ttgcaaactc tttgaaatct
     7141 tgctctctca gactacgacc tgcaataatt tcattcttaa cagcgtccat gtaagttctg
     7201 tttccacctg tcaaattagc attctcaacc tttttatctt gataggtcaa gatggcattc
     7261 gttgaattgg ttacatagta actatccact cccttcagtt tagctgcctc ttggacccag
     7321 gattcttgcg gttttggcgg ttcaacagga acttcctctt cctttccaga aaccgtaaaa
     7381 gctgattgtt tctgagtaaa agacccatct ttactttttt taggagagaa aaagacgcta
     7441 atatttttct gagatttagt catatcttta ttgacttgac gagataggga atcacccaaa
     7501 gccataatca caacaactga tgaaacaccg ataataatcc caatcatagt aagcaaagaa
     7561 cgcatcttgt gagccatgat agatgaaaag gcaaatttca gattctgcat cttagttttc
     7621 ctcctttcct aactgagcac tgtcagacga aatgacccca tcccgaatga caatctgacg
     7681 tttggcatag gcagcaatct caggctcatg cgttaccatg ataatggttt ttccttcttt
     7741 attcaaatca accaataatt gcataatttg gttacctgtt ttggtatcca aggctcctgt
     7801 cggttcatcc gctaggataa tagaaggatt gtttaccaag gcacgcgcaa tggctacacg
     7861 ttgcttttga ccaccagata attctgaagg taaatggtga ctacgttctg tcaattcaac
     7921 cttgtctaaa tattcctcag ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat
     7981 caagggcaat tctacatttt gcagagcatt gagcttcgat agaagaaaga actgctgaaa
     8041 gacaaaaccg atttgttggt tacggacctt agccagttgt ttttcaccaa gcccagccac
     8101 ttcttgacct tcaagataat attctccact ggttggtgta tccaacatgc caatcgtatt
     8161 catcagagtg gacttaccag acccagatgg tcccatgatg gctacaaatt caccctcatt
     8221 cacttctaga ttgatatttt tgagaacctg caattcttgg tcaccattac ggtagcttct
     8281 gaagatattt tttagactaa ttagttgctt catcagtctt cacctctttt ccttcttcca
     8341 aggaagatgt tggattactg atgaccttag caccattcgt taaaccagaa gtgatttctt
     8401 gattttctgc gtcagcattt cccaatgaaa cctcaacttt tttagccttt tgttgttcat
     8461 ccacaatcca gacataattt ttactatcat ccattactag actgctaaca ggaacaagaa
     8521 tagccttagt tttgctttta acctcaatgt tgacagaaaa accctgtttc aaatcaccaa
     8581 cctcgcctgt cacatcaata gtataagggt atttagaacc tgtattattt ccggctgctg
     8641 gactagctgc ttcaccattg tttttaggat agtcagaaat atagcttaat ttcccagtcc
     8701 attttttatc aggatacact ttagaagtaa agcttacttc ttgacctaca gaaaggttgg
     8761 ctagattgta ctcagacaat tctcccttga cttgtaaatt ttcattgctg acaatatgaa
     8821 ccataacttg gctcgcccct gttggagatt tagaaacatt gctattgact tcgactacag
     8881 ttccctctag ggtactgaga acagttgttg catccaattg actttgagcc ttgcttaatt
     8941 gcgccgcagc atctgcacgc gcatcacggg catcacccaa ttgagcgtca atagaagcaa
     9001 cagaatttcc agccactgga gttgggcttt gcaccgttgc atcttctcct cctactggcg
     9061 ctggtaactg tggagccgga gctgaagcgg cttcatttcg tgcttgattg agttcattga
     9121 tatgacgatc tgccttagct actgctcgac tagctgaatc ataggccgcc tgcgcttctg
     9181 aactactgta cttgactaaa gcctgccctt cgctgacctt atcgcccaca gaaacaagga
     9241 tttcatctaa atcaccctta ctagcatcaa aataaacata ttgttcattt tttgctgtta
     9301 ctgtccctga caataaaaca gaggatgcca cgcttccttc cttggcaaca acaagatgag
     9361 taggctcatc ttttagagca gtctgagaag gttgtctaaa gagtaaaatc cccccggcac
     9421 ccaatacaac tacactcgca gcaccgattg ctgcatacag ttgccacttt ttagctttac
     9481 cattcttttt cttcataatg aaactccttt tcttttttac aatactttgc tattatacca
     9541 aatttccctc cagcaaacaa tgcagttcag atggaaataa tggcagttca ggattaaaca
     9601 atcgttcgga attttacttt tctattgtac tagatatata atacactatc ttaacttatt
     9661 tggcaagctt ttttgcaatt ttttgtgcgt agcagatttt tgcaatcaaa tcaaaaaaag
     9721 aataaaatat aactatcaaa aaagacgctc tactatttaa aagattacaa aggagttttc
     9781 aatgagagaa tatgatatca ttgctatcgg tggaggtagc ggaggaatcg ctaccatgaa
     9841 ccgtgctggt gaacatggag ccaaagcagc cgttattgag gaaaagaaat taggtggaac
     9901 ctgtgtcaac gtcggttgtg ttcctaaaaa aatcatgtgg tacggggcgc aaatcgctga
     9961 gactttccat caatttggag aagactacgg ctttaagact actgatctta actttgactt
    10021 tgcaacccta cgtcgcaatc gtgaagccta cattgatcgc gctcgttctt cttatgatgg
    10081 tagttttaaa cgcaacggtg tagacttgat tgaaggtcat gctgaatttg tagattctca
    10141 tactgtaagc gtaaatggtg aactgattcg tgctaaacat atcgtgattg ctacaggtgc
    10201 ccatccaagt attcctaata ttcctggtgc tgagctaggt ggctcttctg atgatgtatt
    10261 tgcctgggaa gaacttccag agtcagttgc cattctaggt gctggttata tcgccgttga
    10321 attagctggc gtactccaca cttttggtgt caagacagat ctctttgttc gccgcgatcg
    10381 tcctttacgt ggttttgatt cctacatcgt tgaaggtttg gtcaaggaaa tggaaagaat
    10441 aaacttgcca cttcatactc acaaagtccc tgccaagtta gaaaaaactg ctaaaggcat
    10501 taccattcat ttcgaggatg gtaccagtca cacagctagc caagttatct gggctacagg
    10561 tcgccgtcca aacgttaaga gcttgcaact tgaaaaagct ggagtgactc tgaatgaacg
    10621 tggctttatc caagtagatg aataccaaaa tactgttgtt gagggaatct atgctctagg
    10681 tgatgtaaca ggtgaaaaag aactgactcc agttgcaatc aaggccggac gtaccctatc
    10741 tgaacgtctc tttaacggaa aaactactgc aaaaatggat tactcaacta ttccaactgt
    10801 tgtcttttca caccctgcta tcggaactgt tggtttgaca gaagagcaag ctattaaaga
    10861 atacggtcaa gaccaaatca aggtttataa atcaagcttt gcatctatgt actctgcttg
    10921 cactcgcaac cgtcaagaat cccgtttcaa actcataaca gctggttcag aagaaaaagt
    10981 tgtcggactt catggaattg gctacggcgt tgatgaaatg attcagggat ttgccgttgc
    11041 tatcaaaatg ggagcaacca aggctgactt tgatgcaact gtggcaattc acccaactgc
    11101 atctgaagaa tttgtaacca tgcgttaatc gaaacaaaag aagctgatat aaatatgtct
    11161 gcttcttttt taatgatttt cgaatcaaca aaaaaattat ccaacttgac tagtcacgaa
    11221 aatcggctgt tacagttttt gttaccttgt ttttaaaaaa tggttgacat cgactcctat
    11281 atgagtataa tagttactat acattagaaa agaggttaac tattttgaaa aaagctcacg
    11341 tttatgctat ccctgctatt ggggctgctc tcattgctgc tttggcacaa atcagtcttc
    11401 caattggacc tgttcccttc actctgcaaa actttgcaat cggcttgatt gctactgtct
    11461 ttagaccgag agaggctgta ctttctgctg gactctatct tcttctaggt gctatcggtc
    11521 ttcctgtctt tgcaggaggt ggagctggtt ttcaggcttt agttggccct actgcaggct
    11581 atctttggtt ttatctcgtt tactctggac ttacttcctc tctaaccaac agcaagagtg
    11641 gtgttgttaa gatttttctt gcaaacctct tgggtgatgc ccttgtcttt gtcggcggga
    11701 ttctcagctt gcatttccta gctggaatgg catttgaaaa agctcttgtt gtgggggttc
    11761 ttccctttat cattccagac cttggcaaac ttctggctat tagttttatt agccgtcccc
    11821 tacttcaacg ccttaaaaat caggcttact ttactaacta aaaaaggata ccgagttatc
    11881 atgactcaat atccttttct tttattttga aaacttatac tcaatgaaaa tcaaagagca
    11941 aactaggaag ctagccgcag gctgctcaaa acactgtttt gaggttgtgg atgaaactga
    12001 cgaagtcagt aaccatacat acggcaaggc aaagctgacg cggtttgaag agattttcga
    12061 agagtattag ttgcctttaa aggcatccac catcgtttga aattcttcat ttgagagagt
    12121 aatccctttg cccattttag tatggtctgg actccaagca cgaatatcaa actttgcagg
    12181 ggcaccatta aagctcacac ggttaatttc cttggtccaa cctttttcgt tttcagaaag
    12241 agtcaacaag tgctcttcga tttcaaatgt aaattctgcc attttcttct ccttttttag
    12301 tttcattagt ttattcgtaa aatcttgtag attttaggaa aattttatat aatattgata
    12361 taaaagaagg gaggccaata tgagacataa attccagcaa gttctaaata aaatacatga
    12421 ttttttaaat ggatatgacc aacctgacca gactgaaacc aactccctta cagccactat
    12481 tgaagaggct atccagaaac aaaccgctgt tcaccttatc ttgtctgaga caagctttac
    12541 aggtgacatc atcaaatatg atcagcaagg ccagcaaatt atcgtgaaaa atttttccaa
    12601 aaatgtgagc cggattatcc gtataagcga tattcaacgc ctgcgatttg tcccctcaac
    12661 tgtccaaaca gcccaaaaaa atagatttaa gaaagagtga gatgtagttg cttcatccca
    12721 ctcttttttc ttagcgaatt tgttcaaaat gtaaatgaac tgcgatatga tctccataac
    12781 cacttctttc caagtcacgt tgtaaacgat aggaaatgta gtgttctgca atggtaatgt
    12841 aacctgcgcc caataaacga tgttcaacca tagattgaat catactgata gtcgcacgtt
    12901 ccaccttggc ttcttgtaaa tccaaaacta ccttcttagt gacttgagca agattttgac
    12961 gcaaatcatc tgtcaaaaca taaacagttt gggctgcctt caagatggct tggtaaatct
    13021 tatctggatt aaattcagca atttcgccat tacgtttgat tacttgcata ggtttctcct
    13081 ttattctttg ttttctttga tttctgccag cattttttct tcttctactg tcagttgata
    13141 atgttcaagt aaatccggtc tgcgctcgta ggttttcttt aaactctcgt acaatcgcca
    13201 ctgacgaatc ttttcatggt ggccactcat caatacatct ggcacgacca tgcctcgata
    13261 atcataggga cgtgtgtact gaggatattc taaaagacct gaagaaaaac tatcatcttg
    13321 gtggctagac tccttgccaa tcacttctgg aatcaggcga actgtagcat caatcatggt
    13381 catagctgcc aattctccac cagtgaggac atagtcacct agggaaatct catctgttac
    13441 caaggtctta atgcgctcat cataaccctc atagtgccca cagataaaga ttagctcttc
    13501 ctcttgagcc aaatcttcag cataagcctg atcaaactgc tttccagcag gatcaaggag
    13561 aataacgcgc ggatttttct tttcaatagc atcaaaggaa tcgaaaatag gttgtactct
    13621 gagcaacatg ccctgaccgc ctccgtaggg ctcatcatct acatgacggg ccttttcagc
    13681 attttctcga aaattatgat actggatatc caagagccct ttttctcgag cctttccaac
    13741 gattgagtgc tccagtggag aaaacatctc tggaaagaga gttaaaatat caatcttcat
    13801 cgtctaaccc ttctaagatt tccacatcga cccgtttatt tggaatatca acattgagaa
    13861 ccactggtgg gatataaggt aaaagcaaat cacgtttgcc ttttcgtttg accacccaga
    13921 catcattagc acctggttgc aggatttcct tgatggttcc aaccaagcta tcaccctcat
    13981 agacttccaa accgataatc tcgtgatagt aaaattcacc atcgtctagg tcattcaaat
    14041 cttcctcagc gaccttgaga ctgtatccct tgtacttttc gatagtattg atatggtaca
    14101 tatctttgaa tttaataatg tcaaagttct tctgtttacg gtggctagcg atggtcactg
    14161 tttggacaaa ctgatctttt tcatcaaaca aagccagctc agctcctttt ttaaaccgtt
    14221 cttctgcaaa atccgtcaca gacaagactc gcatctcacc ctgtaatccc tgcgtattaa
    14281 cgattttccc aacattaaag tagttcatct tgtctcctgt aatctccttt tttccatctt
    14341 attctaacaa ttctcgaata atagccgcaa ttttttccga ttctgaccat tgtaaataat
    14401 ggtgattccc tcctaaaatg agtttagtat tggaagtcca atattctgat tctctgtact
    14461 ctttttctct ataaggctga caaaaaacaa atacaggaat atgagcttct atagatacat
    14521 cctcaaaatc ttcctcagta atctctccag atatctgaaa ttctggatct tgattttcca
    14581 actctaagcc tttttcttgc attaattccc agattttttt attcgtttca ggactaaatg
    14641 ttgcttgagt taagttctta aaataaagtt caggaccaca ctcgtcaatc agcctcatct
    14701 gctcttccat ttctggataa ggattttctg aaaaatcagc aaacatgact tttttagttg
    14761 tcggttcaat tgctactaaa gtctgacgct taattggttt ctcgagtaat ttgcaagcta
    14821 aaattccact ccaactatgt gcacaaagta tatattcaga aattcctaat tcttcaagta
    14881 cttcataaac cgcatctgca agattatcta gattttttcc agcttggtca tgaatcggac
    14941 tcctacctgt gttcggaaaa tcaattgtca aataaccaat tgtaggagga ggtttttcaa
    15001 gtataagtga aaaattttca taacttggta gcaaacctgc tccgtttaga caaactagca
    15061 ctttcttttg cttttgataa gtaacagaga ggctaccaat ttctgtagat acttcaaacc
    15121 tcttcataaa gaaa
//
LOCUS       DAVKZB010000040        14581 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKZB010000040 DAVKZB010000000
VERSION     DAVKZB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.040"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(65..1687)
                     /gene="groL"
                     /locus_tag="VXA80_001093"
     CDS             complement(65..1687)
                     /gene="groL"
                     /locus_tag="VXA80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV0353599.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1703..1987)
                     /gene="groES"
                     /locus_tag="VXA80_001094"
     CDS             complement(1703..1987)
                     /gene="groES"
                     /locus_tag="VXA80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV0353600.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2143..2538)
                     /locus_tag="VXA80_001095"
     CDS             complement(2143..2538)
                     /locus_tag="VXA80_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0353601.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2616..3377)
                     /locus_tag="VXA80_001096"
     CDS             complement(2616..3377)
                     /locus_tag="VXA80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0353602.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3410..4036)
                     /locus_tag="VXA80_001097"
     CDS             complement(3410..4036)
                     /locus_tag="VXA80_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV0353603.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4052..4369)
                     /locus_tag="VXA80_001098"
     CDS             complement(4052..4369)
                     /locus_tag="VXA80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV0353604.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4366..4665)
                     /locus_tag="VXA80_001099"
     CDS             complement(4366..4665)
                     /locus_tag="VXA80_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV0353605.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5002..5193)
                     /locus_tag="VXA80_001100"
     CDS             complement(5002..5193)
                     /locus_tag="VXA80_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0353606.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5354..6028)
                     /locus_tag="VXA80_001101"
     CDS             complement(5354..6028)
                     /locus_tag="VXA80_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353607.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLAIIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6034..6480)
                     /locus_tag="VXA80_001102"
     CDS             complement(6034..6480)
                     /locus_tag="VXA80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0353608.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELEAILPRYFSRVSKSTI
                     VNIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6594..7097)
                     /locus_tag="VXA80_001103"
     CDS             complement(6594..7097)
                     /locus_tag="VXA80_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0353609.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWNICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7094..7456)
                     /locus_tag="VXA80_001104"
     CDS             complement(7094..7456)
                     /locus_tag="VXA80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV0353610.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7522..9381)
                     /locus_tag="VXA80_001105"
                     /pseudo
     CDS             complement(7522..9381)
                     /locus_tag="VXA80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9420..9869)
                     /locus_tag="VXA80_001106"
     CDS             complement(9420..9869)
                     /locus_tag="VXA80_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0353611.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKPVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(9993..10709)
                     /locus_tag="VXA80_001107"
     CDS             complement(9993..10709)
                     /locus_tag="VXA80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0353612.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11590..13005)
                     /gene="ply"
                     /locus_tag="VXA80_001108"
     CDS             complement(11590..13005)
                     /gene="ply"
                     /locus_tag="VXA80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV0353613.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13016..13348)
                     /locus_tag="VXA80_001109"
     CDS             complement(13016..13348)
                     /locus_tag="VXA80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353614.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13419..14027)
                     /locus_tag="VXA80_001110"
     CDS             complement(13419..14027)
                     /locus_tag="VXA80_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353615.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSAAQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14040..14498)
                     /locus_tag="VXA80_001111"
     CDS             complement(14040..14498)
                     /locus_tag="VXA80_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV0353616.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4223 a         3394 c         2641 g         4323 t
ORIGIN      
        1 ataaaaagaa gggttagtca ttccctcctt ttgtgttttt tataagttgt tttctataga
       61 aagcttacat catcccgccc atcatacttg gatccattgc tggagctggg gctactggtt
      121 ctggtttatt ggctacgact gcttctgttg tcaaaatcaa gctggctaca gatgctgcat
      181 tttgtagggc tgaacgactc actttaactg gatcaatgat accttgatca atcatgttaa
      241 cccactcgcc agttgctgcg ttaaatccta taccaagctc agcatttttc aaacgatcga
      301 taacgataga tccttcaaat cctgcattgt gagcaatttg acgaacgggt tcttccaaag
      361 cacggagaac aatattacgt cctgttgctt catctcctgt caattccaag gtagcaacag
      421 ctggaatcac attggcaaga gctgttccac cgcctgcaac aataccttct tcaacagctg
      481 cacgagtagc gttgagggca tcttcaatgc ggagtttcat ttctttcaac tcagtttcag
      541 ttgcggctcc gaccttaata accgctacac cacctgacaa tttggccaag cgttcttgca
      601 atttttcacg gtcaaattca gaagttgtag tttcgatttg agacttgata accgcaacac
      661 ggtgagaaat cgcttcagga tttcctgcac cttctacaat aaccgtgcta tctttgtcca
      721 cggtcactct cgctgcttga ccaagagctt caattgtcgc atctttcaac tcaagaccaa
      781 ggtcttctgt gataactgtt ccgcctgtta agatggcgat atcttcaagc atggctttgc
      841 gacggtcacc aaaaccaggt gccttgactg ctactacgtt gaaggttcca cgaatcttgt
      901 tcaaaacaag agttggaaga gcctcgccat ccacatcatc cgcaataatc aagagtggac
      961 gattgctttg gagaatgctt tccaaaagtg gcaagatctc ttggatattg gaaattttct
     1021 tgtctgtaat caaaatgtac ggattttcaa ggtcagccac cattttttcg ctatctgtca
     1081 ccatgtactg tgaaaggtaa ccacggtcaa actgcattcc ttctacgact tcaagctctg
     1141 tttccatacc acgtgactct tcgatggtga tgacaccgtc tttgccaact ttttccattg
     1201 cttcagagat gtactcacca actttttcag aacgagaaga tacggctgca acttgagcga
     1261 tagcttcttt attggcaaca gggatggcgt tgtttttcaa agcttcaact gctgcggcaa
     1321 ctgctgtttc aatcccacga cgaataccga ttggatttgc acctgctgtg acgtttttga
     1381 ttccttcacg gacgatagct tgggtcaaga ctgttgcagt cgtagtcccg tcacctgcga
     1441 tatcattggt tttagaagct acttctgata ccaatttggc acccatattt tcaaaatggt
     1501 cttctaattc aatttctttg gcaatagtca caccgtcatt ggtaatcaat ggtgagccaa
     1561 atgatttttc aagaacgaca ttgcgacctt ttggtcccaa ggttacttta acagtatctg
     1621 caaggatatc gacaccacgg accatagctg aacgggcatc tgatgaaaat ttaatttctt
     1681 ttgacatact tactttctcc ttctattctt caatgattgc caagatgtta gcttcgccta
     1741 cgatgatata cttttcatcg ccatctttga catcaagacc tgcgtgggct tcaactaaga
     1801 cacggtctcc agttttaaca cttggagcaa ccaagtcacc gttcaaggta cgaacacctt
     1861 gtccagtagc cacaacttgg gctgttttgg ttttttcttg ggctgaacct gcgagaacaa
     1921 agcctccaac agtttgttct ttttcttcta cttttaagac caaacggtcc cctaatggtt
     1981 tcaacatctg atttcctcca taatgagata gatattatta gcactcttta tacctgagtg
     2041 ctaattcata gttctattgt atcacttagt cagaaataat caagaaaaaa gtctgacttt
     2101 ctcaagataa aaagcctgag accaactcag actttttaat tcttaaaatg gcaattcttc
     2161 ctcttccaag accaaatctg ccaaatcttg gcctgcatta ttttcacgca tagcacgttg
     2221 ggcacgactt tccaagagtt ggaatcctgt cacaaggact tcggttacat aattcatctg
     2281 accatttttc tcaaagcgac gggtacgcaa ttctccatca acggaaatga gactaccttt
     2341 ggttgcgtag cttgccaaag tttctgctag tctgccccat aggaccatat tgacaaaatc
     2401 aacttcacgt tcaccgtttt ggtctttgta acgacggttg acagcgatag ttgctcgcgc
     2461 tactgacttg tcattgttgg ttttgtgcaa ttctggtgta gacgttaaac gtccaatcaa
     2521 gataacttta ttatacatat tttcttcctc ctacttatct attcgtagga aatcaaaaaa
     2581 agttacagaa atttgtaact tttcgagaaa attttttatt ttttatgaac catgaaacct
     2641 gtcgcctgtt gattggccat aatggtcata tctgtaatct gaacacgacg aggttgacta
     2701 gtcacataga ctactgtatc tgcaatatcc tgagcttgca aagcttctat tccttggtaa
     2761 acggacgcag ctcgttcttt atcaccatga aaacgcactg tagaaaaatc tgtttcgaca
     2821 attccaggct gaatggttgt caccttgata tccgttgcga tggtatcaat tcgcagtcca
     2881 tccgaaaagg tcttaaccgc agccttggta gctgagtaaa cagcggcacc agcataggcg
     2941 tagattcctg cggttgaccc catattgata atatgacctt gattgacttt tatcattgct
     3001 ggcaagaaac agcgagtaac cgccatcaga cctttaacat tggtatccaa catggtcaac
     3061 atatccaact cctcatagtc ttgataggga gccaagtcaa gagccagtcc ggcgttgttg
     3121 accaagatat caatcctccc tatcgtttct agaatatcag agcaaacagt cttcaccatg
     3181 accatatccg tcacatctag tggaaaagtc cagacggttt gatttggaaa gcttactgca
     3241 aactccgact tgaggatttc taatctgtct atccgtcgtc ctgttagaac gacatcctca
     3301 ccctgctcca gataagcacg cgcaatcgct tcaccgattc ctgaggttgc tcccgtaatc
     3361 actacatttt ttgccatctt atttccttct atcagatgct gacaagttcc taggcagtcc
     3421 agtgtttcgc tgggtcgaac ggtgttccga caacttggtc ttctgataat tcaagcaccc
     3481 cacgtttttg cggagcattt ggtaaatgca attcacgagg actacacatc atcccaaaac
     3541 tcttttcacc acgaagttcg cctgggaaaa tgaaattccc ttttggcatc atggcaccag
     3601 gaagagccac aatggttttc aacccgacac gcgcattggg agctcctgca acgatttgta
     3661 ctgtcttgtc acttgcgact gcaacttggc agatgttgag gtggtcacta tctggatggg
     3721 ctaccatctc gacaatttca ccaacaacaa acttaggttc cttatcatta acaatttctt
     3781 ctgtaaaacc ttccgcctgt aattcttggt tcaaacgagc gacttgctca tctgataaaa
     3841 agacttgacc gcgctctgca atttcaaaca agctcgaaac ctcaaaaatg ttccaagcta
     3901 ctgtttcccc attttctttg aggaaaacac gggctacctt gcctttgcgt tccacattca
     3961 gtttggcatc tccgctattt ttcacgatga ccataaggac atcaccgaca tgttctttgt
     4021 tatacgtaaa aatcattgtt tcctttttct cctatttcaa tcctgctaaa aaatcgttaa
     4081 tttgctcctt acttttacgg tcgcgattga caaaacgacc gatttccttg tccttttcta
     4141 gaacaacaag gctaggaatt ccgtaaacat cccagagttt ggccaaatcc atatactgat
     4201 ctcggtccat tcgaataaag gtgaactctg gattggtctc ttcaatctct ggtaaggcag
     4261 gataaatata acgacaatcg ccacaccagt ctgccacaaa aaggaagacc ttcttgcccg
     4321 ctttttccac taaagatgct aattcttcta aacttgctgg ctgtatcata agacttcctc
     4381 ctcatagact aggtcttcat tttcatagac aaaggtataa tgacgaccat cctcaaaaat
     4441 gacgccacca accaagctct ccagactgct ttcgtaaact tgaacataga gggtcgcaat
     4501 ttcccccatg tcggaaaaat ggtctcgcac aatctctgtc aactcttcct gagtcttcat
     4561 gagcttacgg tcatctgcaa cttttttcgt ggcaagagca aggcttccga tacctagcag
     4621 agccaagcct gccatccaca tttttttagc tttcatacca ttcattttaa cacaaaaaag
     4681 gcttcaggac aaatgaggaa gcagcagaaa agcaagtaaa aagcctcttc ctttaaggaa
     4741 aaggacttct tatactcaat gaaaatcaaa gaccaaacta ggaagctagc cgcaggctgc
     4801 tcaaagcact gctttgaggt tgtagataga actgacgaag tcagctcaaa acactgtttt
     4861 gaggttgtgg atgaagctga cgtggtttga agagattttc aaagagtatt attcttattg
     4921 ccaggcacct aagttgccaa cgtagtaacc atcaggtgtg taggtattgc gaagcatctt
     4981 acctgatgaa gccagataat actacttgcc attgtctttg acccaatcat tcgcaatcat
     5041 ggaaccagaa gaacttacat aataccattc tcccttgtca taaacccaag tactgacttt
     5101 catggttcct gagcaattaa aggcaaaaaa actgtccaat atcattcgtt ttttaaaagc
     5161 atttgacact acatcatcta cgctgtagta catagataca tcaaacgtaa aagccaaaat
     5221 atcgaaaaat atacaacttt taaaggcagc atttcatttt ttaaagtcta acaaccatag
     5281 aactgaaatc agagcaaggc atcaatttcc tactcttagt acgtctattt tgatggttga
     5341 agtgaagatt tttttattta acgtagacaa tttccaactt cccaaaagcc acatctccac
     5401 ggatagtcaa ggttttgctg gttggggcta caggagcgtc agccttagct gcaccaaagg
     5461 aggtttctac tttcaaatct acacgccagt gttgtggaac atagacggtt gcatttccga
     5521 aggccacttc aatatttaaa gttgcaaaat cacctagcat ctctgcatta tcatagtaga
     5581 ttttagcatc cccaaaagcg acttccactt ggtcatctac gagatcttga tcttgcttat
     5641 agaaggtccc gctaccaaaa gcgacttcct tatccgtgac gactgttttt ttcccattgt
     5701 accaccattt tttttcattc cagaacttac ttgaatgcgt cagataacca acaccaagta
     5761 ccaccaagat gctagcccaa ataagagaat gattggtaac tggtaacaag tcataagcgt
     5821 aatttgcaat gatgatcgcc agtaaacctg taaaaactgc cgaagtgaga tgacgtctaa
     5881 ggatggactc aatggactta taagcaaaaa aaacaatacc tagtaaaggc catattttac
     5941 catccaaaga aggaattcca aaattccctt gcagcaagat ccaagctgct aaaaccaata
     6001 aaacaatacc aaatgctttc tttttcatgt tttttacctc atgtttctta gattttcttt
     6061 taggagggat tggtaatgcc gtgacacatg aacctccttg tgggtttgat aaaaggaaat
     6121 ggtgcccgtt cctgaaaagg acttgtccac cgaatagatt tgccgaatat taacgatcgt
     6181 tgacttggat actcgactaa aatagcgagg aaggatagcc tccaactcat agagcttgag
     6241 gcgaacctca taagcttcct tctggttatg agcgtagatc ttgctccctt ctgtttcaaa
     6301 gaagagaatt tctgacaggt ctagataata ttcacctgtc cccttgtaaa aagtcaacct
     6361 cggagccttt gactcttgca agagccgttg caagtctgca atttcatctg tcaaagtggg
     6421 ggtcttgatg acaatttcag tctcctctaa attgccgtca atctcaattc gtaacttcat
     6481 cacatctcct ttctgactct actatatcaa agctaagtta ggaatacaag ggcaaaaaag
     6541 caagtggtca atttgaagca gtaagtggtt gtaggactag cttaacttac atgtcagaat
     6601 aggaagaaaa ggaatcccca caccaaacca caagcatagc caaccaagac atccttggga
     6661 taatgaacac cccctaaaac cctcaccaac cccagcaaag ctgaaaagat caagcatact
     6721 aagccaatag gtagactagc atgcagacag gccatggaga tgatagttgc cgaaaagaca
     6781 tggcgactgg gcatcgaccg tcccgaacta tccttatcaa gaaggggaca gatattccaa
     6841 gtttcataag gtctcgggtg attgattttc ttacgaaaca gggacaaggc cacaaaacct
     6901 gatgcaggaa taagcacata aaccccaact tgctgaccaa gtcctagttg caggtaagtg
     6961 gtgactagca agatcagata gaccaggggc atggcgactg tcatcaaacg attgaaactg
     7021 cgtaatagga ataaaagaat tggacgacta gttagtctgg atctgatatt tcgataccat
     7081 tcttgataat ttttcattta tgcttctcat acttttctag aacttgttga gatgttttcc
     7141 ccttggcaag ccaatccact agcttgtcca agcgacgtat ttcctgcatc agaggatcat
     7201 caatttcctc aatacggaca ccgcaaatct tccctgtaat tgcaattcgt tcagggacat
     7261 aatatggtgc ttgtcgaaaa aaatctccgt aagtaacatc cctatccaga caagcaagaa
     7321 cgtcactcac ttcataccct gtcaaccaac tggtcacttg gtgaactgac tctgcctttc
     7381 caccctttcg ctcaactttt gcaatcaagg ctaagtagac agcagcaaat ttcatattgt
     7441 ataatttctg actcatactt ctctctttct atgtgtacta gtataaataa gaaaaagaag
     7501 gtcgtcaagc cttcttttga tttattcttc tgcttcatct tctgtaaatt gactattgta
     7561 caagtcagcg tagaagccac cttgcgccat cagttcctca tagttgcctt gctcgatgat
     7621 atttccatct ttcatgacca agatcaagtc tgcatttcgg atggttgaca agcggtgggc
     7681 aatgacaaag gatgtgcgtc cttccatcaa acggtccatg gctttttgga tcaattcctc
     7741 tgtccgtgtg tcaacagaag aagtcgcctc atccaaaatc aaaagcggtg catccttaag
     7801 aagggcacga gcaatagtca atagttgttt ttgtcttaca gacaaggtca cggtgtcatc
     7861 caagatggta tcatagccat ctggcaaggt cataataaag tggtgaattc ccacagcctt
     7921 actagcttcc atcattcgtt catcactaat ccctatttga ttatagatga gattgtctcg
     7981 aatagttcct tcaaagagcc aagtatcctg caagaccatt gaaaaggcat catgcacttc
     8041 tgaacgcgtc atagccttgg tatccacacc atcaatgcga atacttccct tatcaatctc
     8101 atagaatttc atcaaaagat tgacaatggt tgtcttacca gccccagtcg gcccaacaat
     8161 ggcaaccttt tgaccagcat gagctgtcgc agagaagtca tagtcttgaa cattgacacc
     8221 gtccaccaga atttctcctg ctgacacgtc gtagaaacgt ggaatcagat tgaccagagt
     8281 tgatttacca gaacctgttg acccaataaa ggccactgtt tgaccagttt ctgctttaaa
     8341 gctaacatgt tcaataactg cctccgaatt tgccgcatag cggaaggtca catccttaaa
     8401 ctcgacctga cctttgaagt tttcatcagt cagctgcact tgaacagggt tttggataga
     8461 agaatgcaaa tctaaaactt gattaatccg cttagcagag accatagttc ggggaagaac
     8521 gatgaagagt gctcccatga gaaggaagcc catgacaacc tacatggcat aagacatgaa
     8581 aacaatcatg tcactaaaga gaggcagacg cgctatcgga gcagcgtcgt taatcacata
     8641 ggccccaatc cagtaaatcg ccacactcaa accacttgaa atccccatca tgataggatt
     8701 caaaatagcc ataagacggt tgacaaacaa attcaaacgg gtcaattcat catttactgc
     8761 tgcaaatttt tcattttgat aatcctctgc attgtaggca cgaacgacac gaatacctgt
     8821 taaactctca cgagtgatac tgttcagttt atctgtcagt ccctgaatca aggactgttt
     8881 tggaaaggct agcgtcatca aaacggtcgt catcaggacg ttgataatca ctgccacaag
     8941 tacggcccag agccagtatt ctgaatgacc taaaatcttc ccaatagccc agatagccat
     9001 aattgaacca cgcgttacca cttgcaagcc catagtaatc aacatttgaa cttgagtaat
     9061 gtcattggta gtacgcgtca agaggctagg aattgaaaat ttcttaatct ctgtctgcga
     9121 gtaatccaaa actcggttaa aaatatcact tctcagccta ctagtataag aagccgccac
     9181 tcgggatgca aaaaatccaa ctgcaactac ggacaagaag gcaagaaagg acattcccat
     9241 catcatgctt gccgactgcc acaactcatc taaattagtt tcttgactac ctagcaaatc
     9301 cgtaattttc gagatatagg tcggcacttc caactctaga tagaccgaaa agcaagtaaa
     9361 gagaatggct agtaaaatca tcccccattc ttttctacta attcttttgg ctaatttctt
     9421 tattctctcc tcctattccc ttgatatttt gcctgtagtt gaccgagaac cttctcaaaa
     9481 atcagtaatt catcttcatc aatgtcttcc atcaactgct tgtctatgcg ttcaaaaaaa
     9541 gccttaacct gttgcatctg agaacgtgct ttgtccgtca gacgaacaaa cttagcccgc
     9601 ttatcaacag gactcgcctc caattccacc aaaccatttt gcactatacg cttaaccaga
     9661 ttactagcaa caggcttggt aatattgagt tcctgctcga tatctttaat caagaccaag
     9721 tcttggtttt tctcgcgatt atccaaaaaa cgcacaacct gaccttgcgg cccacccata
     9781 aattcaatgc cgcaacgttt ggcttccttt tgcaccatca ggtgaatttg atgaccaaaa
     9841 cgcttaaaga ctaacatcgg tttatccata atctccccct tctaaataaa aatagttctc
     9901 tggagaataa ttaaatttct atgagaacta ttttcttgat taaaaaaatc ccaagtgatt
     9961 ttctcactta ggatcatgtt ctataggtta aattaaaacc catctacgtt cgtataaatc
    10021 ttttggacgt cttcgtcgtc ttcaagaacg ctgtaaagtt tttcaaaggt ttcaaggtct
    10081 tcgcctgaca attccacttc tgactgagga atcatttcca attcagtcac ttggaattct
    10141 tcaataccag actcacggag agcaacgata gccttgtgaa ggtcagttgg cgctgtgtaa
    10201 actgtgattg taccttcttg tgcttctacg tcatccacat ccacatccgc ttcgagcaat
    10261 tgctcaaaga ctgcgtccgc atcttcacct ccaaatacaa taacaccttt gttgtcaaaa
    10321 aggtaagaaa cagaacctga agcgcccatg tttccgccgt ttttaccaaa ggctgcacgg
    10381 acattggctg ctgtacggtt aacgttagaa gtcaaagtat ccacaattag catagagcca
    10441 tttggcccaa aaccttcgta acgtccttct gtaaaggttt cgtctgtgtt tcctttggct
    10501 ttatcaatcg ctttatcgat aatgtgtttt ggcacttggg cttgtttagc acggtcgata
    10561 acgaatttca aagctgagtt tgattctgga tctggatcac cttttttagc tgctacatag
    10621 atttctacac caaattttgc atatacttta gagttggctc catctttagc cgttttcttg
    10681 gctacgatat tggcccattt acgtcccatt aggaatctcc ttttttcaca ttttaatctt
    10741 tcttattata acacaagttt ttttgatttt cactagagga aatggatttt attagcaaat
    10801 caagctagga tagcacttta cctgctaaga tggtcttacc tttctatctt tatcaacaga
    10861 cactcatcca cattcaaaaa acaaactaga ccattatctg caaatagaaa gtttcagcca
    10921 agtttgacaa agtcagctca aattactgtt tgaagtttgt agatataagc gacaaaaaca
    10981 atcatactgc accttttgtt gacagtctac tccagacata tcatagttca agtaaatact
    11041 ttgaaatcca acagttctta taggcgctat tgttttccaa gaaatcaata gaagagtttc
    11101 taagcaaacc tctaatactc aataaaaatc aaaaagcaaa ctagaaagct agccgcaggt
    11161 tgctcaaaac actgttttga ggttgcggat agggctgacc tggtttgaag agattttcga
    11221 agagtataat ttacgtgttc ccaagatgga gaaattagac tagtacactg gcacttctaa
    11281 accattgcta gcaattgatt tgttcatatt taatttcatt ttttccataa atgggtatta
    11341 gatataaaca gcaaaatatt tccgatacgt gtcattcttg aatttccaat catctaaaac
    11401 aagtaaagga taatcaatcc cctgtatatc aaggaattgg ctaccctttt tactttttta
    11461 cacattctgt ttgatagatt cattttaaca tcacgagcat actccaatgg aaatcgctag
    11521 gcaagagata aactttcaga tatccgcaga gagatcatcg cctctttttg tcgcaagcat
    11581 tctcctctcc tagtcatttt ctaccttatc ttctacctga ggatagagag ttgttcccca
    11641 aatagaaatc gtccgcttac gcactagtgg caaatcggtt ttttcataaa ccgtacgcca
    11701 ccattcccag gcaagcccgg tacactctct aattttgaca gagagattac gaacattccc
    11761 ttttaaagga atactagtgg taaagtgagc cgtcaaatcc tgcccatttc tgtcccaagc
    11821 cttaggagtc aagacttcct taccttgatg atcataggat aattcatccc aagtaatata
    11881 atattgggca acataggcac cactatgatc cagcagtaaa tctccgtttc tgtaagctgt
    11941 aaccttagtc tcaacatagt ctgtactgtt ttgaaaggtc gcaactacat tgtcacgtaa
    12001 aaaagaagtt gtataggaaa tcggcaagcc tggatgatct gctgtaaagc gactgccttc
    12061 ttgaatcaag tcctctacca tatccacctt gcctgttaca actcgggcac ccgaacttgg
    12121 gtcgccccct aaaataaccg ccttcacttc tgtattgtcc aaaatctgct tccactctgt
    12181 ctgaggagct accttgactc cttttatcaa agcttcaaaa gcagcctcta cttcatcact
    12241 cttactcgtg gtttccaact tgagatagac ttggcgccca taagcaacac tcgaaatata
    12301 gaccaaagga cgctctgcag aaattcctct ctgttttaaa tcctctaccg ttacagtatc
    12361 ttgaaacaca tctcctggat ttttaacagc atctacgctg actgtataat aaatctgctt
    12421 aaaattaaca atctgaatct gcttttcgcc tgaatggaca gagttaaaat caatatcaag
    12481 agaattccct gtcttttcaa agtcagaacc aaacttgacc ttgagttgtt ccatgctgtg
    12541 agccgtgatt ttttcatact gcattctagc tgggacatta ttgacctgac cataatcttg
    12601 atgccactta gccaacaaat cgtttaccgc tccgcgaaca cttgaattgc tggggtcttc
    12661 cacttggaga aagctatcgc tacttgccaa accaggcaaa tcaatactat aagtcatcgg
    12721 agcacgatcg accgcaagaa gagtgggatt attctctaac aaggtctcat ccactacgag
    12781 aagtgctcca ggatagaggc gactgtcgtt ggtagctgtt acagaaatat cacttgtatt
    12841 tgtcgacaag ctccgcttct ttctttcgat aacaacaaac tcatcgggta gctgattacc
    12901 ctctttgatg aaacgatttt caatactttc tccctgatgg gtcaagagtt tctttttatc
    12961 gtaattcata gctagtataa agtcatttac tgctttattt gccatcttct acctcctaat
    13021 aagttcctgg attgagttgc ataaactcag acttgttcag cgaaatcagc cgtggttgga
    13081 ctaagtaatc caaaatttcc tcgtacaatt cttctgagac attgcgtcgc cgtctggcta
    13141 gataagaagt cggaatgacc gtattatcca acataaatac cttatctaag tcaatcaagg
    13201 ttggtcttgt aaaaggatta cgagctagat ccggctcttc tatcataaag ttcttgacca
    13261 aacgtctggt caagagagct ggtttgaagg tctgattttt aaccaactct ttgtttttag
    13321 tcatgctgtt gtcaatacag atatacatat gattcttcac agccaaatcg ctactaatag
    13381 tcggaaaagg caaataaaga gctacaacat ctcctctctt aatcaagcaa gagcaccccc
    13441 ttttctccta atgtaacata gacaggattg accaagtctt ctgattgact cagaatttcc
    13501 aaagtttgag tttggcgcgc tgtcaattta gtagcatctt gtctcttcaa tacaaaatgc
    13561 ttgtcgccaa taaccttgac aatataatcc ttctccaaag ctgactggta aatccacatc
    13621 agatgttgtc tgtcctgaga actcaagaga gaaggatttt caagcctccc gatagtctga
    13681 taaaaatcaa aaacaggagc taactcctgc caatctgatt ggctagttgt caaggctaga
    13741 aaaagggctt tgcgagctga tacttcttgg ttagccttga gagttacttt cccctccaag
    13801 ttttttagaa atcgggaaac tccagaaagc aaatttttct ctaactgcga gaaataaaaa
    13861 cctttcgttc ccagacataa gtctttcatg tcgctttctc tagcaaataa gagctcaaac
    13921 atttgatagt aaaagaaaaa tatctggcac tgggccgcgc tcatcttttc cttatcggct
    13981 tcttttttta accagagcaa gggcgacagg tagctggatt gagacatttc ctctacctcc
    14041 tactcttttt taactggagc atctgcacta gctgccactt cttttgactg gatactttcc
    14101 cactggttaa tctcctctga gataagacct tcgcatgtct tgacaaatag ggcaaaagcc
    14161 ttggtctttc ctgcatattt ctccgtttgg cattgataga ggaatttttc tttctccagg
    14221 agttgcgcag ttttttggta agaaatccaa ttttcctttg cattatacaa attgataatc
    14281 ccctcacaca gcaagccgag actggataag gcaaccgaaa tcaaacggta gcgatcacct
    14341 ggcataggaa tagcacaaaa gacagctatg aggaaacctg ccacgatttc tgttattttt
    14401 aataccttat agcgcctacg atgttgaacg cttttcttta aaaaatgagc tatctgtacg
    14461 tctaatcgct ctgtcaggta catttcttct ggcgtcatat tcgtaactcc tttcatttac
    14521 tttgataatc atagtataac atatttgaat atcaaagtat agtaaaaact ggactataac
    14581 t
//
LOCUS       DAVKZB010000041        14233 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKZB010000041 DAVKZB010000000
VERSION     DAVKZB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.041"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            8..691
                     /locus_tag="VXA80_001112"
     CDS             8..691
                     /locus_tag="VXA80_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0353617.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            691..1425
                     /locus_tag="VXA80_001113"
     CDS             691..1425
                     /locus_tag="VXA80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0353618.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1579..2436
                     /locus_tag="VXA80_001114"
     CDS             1579..2436
                     /locus_tag="VXA80_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV0353619.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2573..3394
                     /locus_tag="VXA80_001115"
     CDS             2573..3394
                     /locus_tag="VXA80_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV0353620.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            3408..4280
                     /locus_tag="VXA80_001116"
     CDS             3408..4280
                     /locus_tag="VXA80_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV0353621.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQDRVSKDKLV"
     gene            4562..5245
                     /gene="rpiA"
                     /locus_tag="VXA80_001117"
     CDS             4562..5245
                     /gene="rpiA"
                     /locus_tag="VXA80_001117"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV0353622.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            5259..6470
                     /locus_tag="VXA80_001118"
     CDS             5259..6470
                     /locus_tag="VXA80_001118"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV0353623.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            6472..7014
                     /locus_tag="VXA80_001119"
     CDS             6472..7014
                     /locus_tag="VXA80_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV0353624.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKVQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            7030..7839
                     /locus_tag="VXA80_001120"
     CDS             7030..7839
                     /locus_tag="VXA80_001120"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0353625.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKILALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            9086..9943
                     /locus_tag="VXA80_001121"
     CDS             9086..9943
                     /locus_tag="VXA80_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0353626.1"
                     /translation="MSADLVLLGGNKPAQADDIRKVVGELAKDISITKLYMTGHSLGC
                     YLAQIAAVEAYQKYPDFYNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAV
                     SGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQA
                     TLDKHGYRDPKLDKVRFFKKQALPRSSSQPSAELMENIALGKQVTQSSTVFGGDARRA
                     VDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVIL
                     LDSSGKEIE"
     gene            9968..10480
                     /locus_tag="VXA80_001122"
     CDS             9968..10480
                     /locus_tag="VXA80_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV0353627.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            11137..11847
                     /gene="deoD"
                     /locus_tag="VXA80_001123"
     CDS             11137..11847
                     /gene="deoD"
                     /locus_tag="VXA80_001123"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0353628.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(11870..12394)
                     /locus_tag="VXA80_001124"
     CDS             complement(11870..12394)
                     /locus_tag="VXA80_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV0353629.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(12447..12683)
                     /locus_tag="VXA80_001125"
     CDS             complement(12447..12683)
                     /locus_tag="VXA80_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV0353630.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(12751..13671)
                     /locus_tag="VXA80_001126"
     CDS             complement(12751..13671)
                     /locus_tag="VXA80_001126"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV0353631.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            13861..14228
                     /locus_tag="VXA80_001127"
                     /pseudo
     CDS             13861..14228
                     /locus_tag="VXA80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4308 a         2468 c         3197 g         4260 t
ORIGIN      
        1 tgtgactatg aatttttctt ttttacctaa gtatttacct tattttaact atggggctgt
       61 tgtgacgatt cttatttcta tctgtgttat ctttttggga actattttgg gtgttgtctt
      121 ggcttttggg caacgttcaa agtttaaacc gcttgtttgg ttggccaact tgtacgtttg
      181 gattttccgt gggacaccga tgatggttca aattatgatt gcctttgctc ttatgcatat
      241 caatgctccg actattcaga ttggaatttt aggtgttgat ttttcgcgtc tgattccagg
      301 gattttgatt atctctatga atagtggtgc ttatgtttcg gagactgttc gtgccggaat
      361 caatgcggtt ccaaaaggtc agctagaggc ggcttattcg ctagggattc gtcctaaaaa
      421 tgcgatgcgt tatgtgattt tgccacaagc agtcaaaaat atcttgccag cattggggaa
      481 cgaatttatc accattatca aggacagctc cctcttatca gctattgggg tcatggagtt
      541 gtggaatggg gctacaacag tttctacaac aacctatcta cctttaacac cacttttatt
      601 tgcagcattt tactacttga ttatgacctc tattctgaca gtagccttga aagcttttga
      661 aaaacatatg ggacaaggag ataagaaata atgacagaaa ccttgataaa aattgaaaat
      721 ttacataaat cttttggaaa gaatgaagta ttgaagggca tcaacctcga gattaaaaga
      781 ggagaagttg tcgttatcat cggtccttca gggagtggga aatctacctt gcttcgctct
      841 atgaatttgt tggaggaagc aaccaagggg aagcttatct ttgagggagt cgatattacg
      901 gacaagaaga atgacctgtt tgccatgcgt gagaagatgg gcatggtttt ccaacaattt
      961 aacctctttc ctaatatgac tgtgatggaa aatattacct tgtcacctat caagactaaa
     1021 ggtgaaagca gggaagttgc tgagaagaga gcccaagagc ttttggaaaa agttggtttg
     1081 ccagataagg cagacgctta tccacagagt ctgtcgggtg ggcagcaaca acggattgcc
     1141 atcgcgcgtg ggttggctat ggaaccagat gttttgctct ttgacgaacc aacttcagcc
     1201 ttggatcctg aaatggtagg tgaggtattg gctgttatgc aagaccttgc caagtcaggg
     1261 atgactatgg ttatcgtaac acatgagatg ggatttgccc gtgaggtggc agatcgtgtt
     1321 atctttatgg cagacggtgt ggttgttgaa gatgggacac ctgagcagat ttttgaacaa
     1381 acccaagaac aacggactaa agatttcttg agtaaggttt tataatctat tttaaaattt
     1441 caagacaaga ttagtgaaaa gttcaaccag agctttttct tatagtttga aactatagga
     1501 taacctagga aagaagtgtt agagggcact agcagttttt ggtataatgg aagatatttg
     1561 aaagaaaaga gaagtgatat gacacagatt attgatggga aagctttagc ggccaaattg
     1621 caggggcagt tggctgaaaa gactgcaaaa ttaaaggaag aaacagctct agtgcctggt
     1681 ttggtagtga ttttggttgg ggataatcca gccagccaag tctacgttcg caacaaggag
     1741 aggtcagctc ttgcggctgg ttcccgtagc gaagtagtgc gagttccaga gaccattact
     1801 caagaggaat tgttagacct gattgctaaa tacaatcagg atccagcttg gcatgggatt
     1861 ttggttcagt tgccattacc aaaacacatt gatgaagagg cggttctatt ggctattgac
     1921 ccagaaaagg atgtggatgg tttccatcct ctaaatatgg gacgcctttg gtctggacat
     1981 ccagtcatga ttccatcaac acctgcagga attatggaaa tgtttcatga atatgggatt
     2041 gaattagaag gtaaaaatgc ggttgtcatc ggtcgttcta atatcgttgg aaaacccatg
     2101 gctcagcttc ttttggccaa gaatgcaaca gtgactttga cccactcacg cacccataat
     2161 cttgctaagg tggctgctaa agcagatatt ctggtggtcg caatcggtcg tgccaagttt
     2221 gtgactgctg actttgtcaa accaggtgcg gtagtcattg acgttgggat gaatcgtgat
     2281 gaaaatggta agctctgtgg ggatgtggat tatgaggcag ttgccccact tgctagctat
     2341 attacgccag tccctggagg tgtcggccct atgaccatta ctatgctgat ggagcaaact
     2401 tatcaagcag cacttcggac attggataga aaataagaga aaaatcgctg aagagagtgt
     2461 attttctata gctatattta aaatggcaga aatgaatatt aaattttaga tataagatta
     2521 caaaaggagg tctgcgcctc ctttttgttg tataatggag tgaggtgatt agatgatttt
     2581 aaaaatttat aatagggaat atagtttaca atgggatgga atatactacc tagcactaat
     2641 tgattatcca aatattcaag agtgggaatt agaaaaaatt gctaaattta tagcttacga
     2701 aaaacttcat aaacgtcaaa caagtattga gtgtgctgat tcttgtttaa aaaaagaaat
     2761 tttagattac atctgtcagc atccctttct gccaccattt acccctacag ataaaagagt
     2821 agcctcgact tatgatctac ataagaggtt agtgacttca gactactgta gtcatactac
     2881 gactatagat gcagcgattt ctatctttaa aacaggtcag ctcttatctg ctgtgaaagc
     2941 ctttaggcga gatgctgaag agttggtttt ggatagtaga aatgctgcat cggatccgat
     3001 agattatttt gattatgtca tgttagggtg gtcaaataca agttctggtt atcgattagc
     3061 gatggagcgt ttattaggtc gagctccttc agagaaagaa ttacaagaca agtttattcc
     3121 tggagtgagt tttcatttta tctatacaga tttgattaaa gttactggtt atatttttga
     3181 tggctaccat gttgcaaaaa ttaaggacat gttgaattta tttagtgagt tgtatatttg
     3241 tattattcca actcataata agagccaatt tgaaaatatt attccaacta aaatacaaga
     3301 tagggtgcat tatcttgact atgctggaga agacttagaa gagtggacta agaaagtcta
     3361 tcaagttgtt ttaaaacaat cagataaagg atagttgagg aagaacgatg aaagtgattg
     3421 atcaagcctt actagaaaaa gttattattg aacgttctcg tactagtcat aaaggagact
     3481 atggccgtct gctgtttctt ggtggaactt atccttatgg tggtgccatc atcatggctg
     3541 ctttggcagc tgtaaaaagc ggtgcaggat tggtgaccgt tggaacagat agggaaaata
     3601 ttccagccct gcacagccat ttacctgagg ctatggcctt ttctcttcaa gaccagcagt
     3661 tgttaaaaga gcaattggag aaggcagaag ttgtcttgct ggggcctggt ttacgagacg
     3721 atgcttctgg agaaaatcta gtaaaacagg tctttgttaa tttaagccaa aatcagattt
     3781 tgattgtaga cggaggtgcc ttgaccattc ttgctaggac aagtttgtca tttccctcaa
     3841 gccagcttat cctaactcct caccaaaaag aatgggaaaa attatctgga attacgattg
     3901 aaaagcaaaa ggaagatgca acggctagtg ttctgacttc cttccctcaa ggaacaattt
     3961 tggtggagaa aggcccagct actcgtattt gggaagttgg ccaatctgat tattaccagt
     4021 tacaggttgg tggtccttat caggcgactg ggggaatggg agatactctg gctgggatga
     4081 tcgcaggatt tgtaggccaa ttccgacagg ccagtctcta cgagcgtgtg gcagtagcga
     4141 cccatcttca ttcagccata gcccaagaac tatctcaaga aaattatgtg gtcttaccga
     4201 cggaaatcag caggtatctt cccaaaataa tgaaaattat ttgtcaacaa gatagggtaa
     4261 gcaaagataa acttgtttaa aatagacaga taaatctact tccttctctt aatttatgat
     4321 tcttgaaatg cgcctgcttt aaggctaaac tagacgtatc aaagttaaga gtttgttttt
     4381 gtaactggaa agagatgaac aaatcaaata cataaaagat aaactcattt ttattcgttt
     4441 ggtaaaaata aaatgcatat ttttaaagaa agattgatga aaaatgtaga tttgatgttt
     4501 ttacttgaat aaaaagcggt ttcatagtaa aataagaact gagaatgatg gaggtaaatc
     4561 ggtggaaaat ctgaagaaaa tggcaggtat caaggctgct gagttcgtga gcgatggaat
     4621 ggtcgttgga cttggaacag gctcgactgc ctattatttt gtcgaagaaa tcggtcgtcg
     4681 tatcaaggaa gaaggattgc agattacagc tgtaacaact tctagtgtga ccactaaaca
     4741 ggcagaaggc cttaatatcc cgctcaagtc tattgaccaa gtagactttg tcgatgtgac
     4801 ggttgatggc gcggatgaag tggatagtca gtttaatgga atcaaaggcg gtggtggtgc
     4861 ccttctcatg gaaaaggtgg tcgcaacacc atcaaaagaa tacatttggg tggtggatga
     4921 aagcaagctg gtcgaaaaac taggtgcttt taaattgcca gtagaagtgg ttcagtatgg
     4981 tgcagagcag gtctttcgtc attttgaacg agctggctac aaaccaagtt tccgtgaaaa
     5041 agacggccaa cgttttgtga cggatatgca gaattttatc attgacctcg ccttggatgt
     5101 cattgaaaat ccaattgctt ttggacaaga attggaccat gtcgttggtg ttgtggagca
     5161 tggtttattc aaccaaatgg tggataaggt aatcgttgct ggacgagatg gagttcagat
     5221 ttcaacttca aaaaaaggaa aatagaaggg ggcataagat gtctaaattt aatcgtattc
     5281 atttggtggt actggattct gtaggaatcg gtgcagcacc agatgctaat aactttgtca
     5341 atgcaggggt tccagatgga gcttctgaca cactgggaca catttcaaaa acagttggtt
     5401 tgaatgtccc aaacatggct aaaataggtc ttggaaatat tcctcgtgaa actcctctta
     5461 agactgtagc agctgaaagc aatccaactg gatatgcaac aaaattagag gaagtatctc
     5521 ttggtaagga tactatgact ggacactggg aaatcatggg actcaacatt actgagcctt
     5581 tcgatacttt ctggaacgga ttcccagaag aaatcctgac aaaaatcgaa gaattctcag
     5641 gacgcaaggt tattcgtgaa gccaacaaac cttattcagg aacggctgtt atcgatgatt
     5701 ttggaccacg tcagatggaa actggagagt tgattatcta tacttcagct gaccctgttt
     5761 tgcagattgc tgcccacgaa gacattattc ctttggatga attgtaccgt atctgtgaat
     5821 acgctcgttc gattaccctt gagcgtcctg cccttcttgg tcgcatcatt gctcgccctt
     5881 atgtaggtga accaggtaac ttcactcgta cggcaaaccg tcgtgacttg gctgtatctc
     5941 cattttcccc aactgttttg gataaattga atgaggctgg tatcgatact tatgctgtgg
     6001 gtaaaatcaa cgatatcttt aacggtgctg gtatcaacca tgacatgggt cacaacaagt
     6061 caaatagtca tggaattgat acactattga agactatggg acttgctgag tttgaaaaag
     6121 gattctcatt cacaaaccta gttgactttg atgcccttta cggccatcgt cgtaatgctc
     6181 acggttaccg tgattgcttg catgagtttg atgaacgctt acctgaaatt atcgcagcta
     6241 tgagagagaa tgaccttctc ttgattactg cggaccatgg aaatgaccca acgtatgcag
     6301 gaacggatca cactcgggaa tatattccat tgttggccta tagccctgcc tttaaaggaa
     6361 atggtctcat tccagtagga cattttgcag atatttcagc gactgttgcc gataactttg
     6421 gtgtggaaac tgctatgatt ggggaaagtt tcttagataa attggtataa gatgacgcgc
     6481 tatgctttgc tggtgagagg tatcaatgtt ggtggtaaga ataaggtcgt catggcggag
     6541 cttcgtcaag aattgacaaa cttgggactg gaaaaggttg agagctacat caacagtggc
     6601 aatattttct ttacttcgat agattccaaa gtccaattgg ttgaaaagct agagactttc
     6661 tttgcagtcc attatccatt tattcagagc ttttctttac tgagtctaga ggactttgag
     6721 gcggaacttg aaaatctacc agcttggtgg agcagagact tggcacgaaa agattttctc
     6781 ttttacactg aaggtttgga tgtggaccaa gtcatcgcga cagttgaaag tttagagctg
     6841 aaagatgaag tgctttattt tggaaaactt gggattttct gggggaaatt ttctgaagaa
     6901 tcctattcta agactgccta tcataagtac ttgctgaagg tgcctttcta ccgccacatt
     6961 actattcgta atgctaaaac ctttgacaaa attggtcaaa tgctaaaaaa ataataaagg
     7021 agacacacaa tgacattttt aaacaaaatc catgaaactg ctactttcct gaaagaaaag
     7081 ggaattgcag cccctgagtt cggtctaatc cttggatcag gacttggaga attggcagaa
     7141 gaaatcgaaa atccagttgt agtagactat gctgagattc caaactgggg ccgttcaaca
     7201 gtagtcggtc atgctggtaa attggtatat ggtgaactgg caggtcgcaa gatcttggct
     7261 cttcaagggc gtttccattt ctatgaaggg aatcctctgg aagtggtgac tttcccagtt
     7321 cgtgtgatga aagttcttgg atgtgaaggt gttattgtaa ccaatgcagc tggcggtatc
     7381 ggatttggtc ctggtacctt gatggctatc tcagaccata tcaacatgac ggggcaaaat
     7441 ccattgatgg gtgaaaactt ggatgacttt ggcccacgtt tcccagatat gtctagggct
     7501 tacacaccag aataccgtgc cactgcccat gaagtggcta aaaaacttaa tatcaagctt
     7561 gatgaaggtg tctatatcgg agttactggt ccgacttatg aaacaccagc agaaattcgt
     7621 tcctataaga cactgggagc agatgcagtt ggtatgtcta cggttcctga agttatcgtg
     7681 gcagcccact ctggcttgaa agttctggga atttcatgta tcactaactt tgcggccggt
     7741 ttccaagaag aactcaatca cgaagaagtt gtagaagtga ctgaacgtgt taaaggtgat
     7801 ttcaaaggct tgcttaaagc gattcttgct gaattgtaag aaaaaagatt taaaaggggg
     7861 agtgcctctg tcttttcagg attgactgcc tatccgtatt aaagaagaaa cagaggaata
     7921 ctatgagctt cttcctgctc ttataactga aagaagcgga agaataggta tgtctgatct
     7981 gatagccagc attgtgaaag acaagattct aggatactag cattagcttc ctagccaagc
     8041 agactagtat gataaggaga gatgagaatg aattgacttt ctgaatttct cagtcttatc
     8101 atatatagca caatgagatt tcgcttgagt ctgcttgtaa ataaacgaaa agaaagataa
     8161 gaaataatga aaattggtca acgaattatg cgctttggca taaaaaatta agtatcggag
     8221 ttgtatctgt tgtagtcggc tttgatttct agctccagct ggaatttcag ccaatgaagt
     8281 aaagcaagat gtaacatctg aagtggtaat aggtgtgcta gattctaagg aggaattgaa
     8341 agagtcagaa aatgatgctc caaaactaga aactcctctt agagaggagc caagactagc
     8401 tcctcaaacg cttccggaag caagtgaagt tcttgaaaac aaaagggaag agtcaaaagt
     8461 agagataaca gagccagctc aagactcacc tattcttgct cctgtagaag aaactaaaga
     8521 agaagctgtg acagaaaaac caacaaatac tcgttctcta actgcagaag atttggtgaa
     8581 gatttccaaa ggggaattgc atttagaaaa tgatttgatt gatgaatctt tctatggtga
     8641 aaaagctctt gatttggaag gggatgatga ccaggatggc atcaaaaaca aagatggtaa
     8701 ggattatcta ggatataaca gtcatccctt gctagcagac agtgatgggg atggtttggc
     8761 agatggggaa gatgataata agaaagaatg gtatgtcaca gaccgtgatt ctcttctctt
     8821 tatggagtta gcttatcgag acgatgatta tattgagaaa attttatatc ataagaatct
     8881 tttccctagt ctctatcttg accgtcaaga acacaaactc atgcacaatg aattggctcc
     8941 tttctggaag atgaaaaaag cctactatac agatagtggc ttggatgctt tcttatttga
     9001 gaccaagagc gaccttcctt atctcaaaga tggaacggtg tacatgttgg ctattcgtgg
     9061 aacgcgagtt aatgacgcca aggacttgag tgcagatttg gttttattag gtggaaataa
     9121 accagctcaa gcggatgata tccgcaaggt tgttggggaa ttagccaagg atataagtat
     9181 tactaagttg tatatgacag gtcattctct tggatgttac ctagctcaga ttgcagcggt
     9241 tgaagcttac caaaaatatc ctgattttta taaccatgta ttgaggaaag tgacaacttt
     9301 cagtgctcct aaagtgatta cttccagaac tgtttggaat gctaagaatg gtttctggga
     9361 tgttggtttg gaaagtcgta aattagctgt tagtggaaaa attaagcatt atgtggttga
     9421 taatgacaat gttgtgactc ccttgattca taataatcgt gatattgtta catttacagg
     9481 taattcacgc tttaaacacc gttctcgtgg ctattttgaa agtccaatga atgatattcc
     9541 taactttaat attggtaaac aagctacctt ggataaacat ggttatcgtg atccgaaatt
     9601 ggataaagtg cgattcttta agaaacaggc tctgcctcga tcttctagtc aaccaagcgc
     9661 tgaactaatg gaaaatattg ccttaggaaa acaggttact caaagttcga cagttttcgg
     9721 aggagatgct agaagagctg tggatggcaa agtcgatggt aactatggtc acaattctgt
     9781 cactcataca aacttccaat ctaagccttg gtggcaagta gatttggcta aagaagaaac
     9841 cattcgccaa atcaatattt acaaccgaac agacactgcc caggatagat tggcaaactt
     9901 tgatgtcatt cttttagaca gttctggtaa agaaattgag taaaaacgta taacatctcc
     9961 taaagatgtg tcagcacaaa ttacgattaa ccataaaaaa gtacgctatg ttcggattga
    10021 gctagaaggc tataatgccc tcagtcttgc agaagttgaa gttttccgct ttatagctac
    10081 gaatgctgaa acggcgacac aagtttctaa gccagttcaa ccaatcagtc agactcctgt
    10141 gaaagataaa acattgacaa ttcaacacag tggagcttac attgcccgct actccataac
    10201 ttgggaagaa gttccagtag ataaagatgg aaaccaagtt gttcgtagtc attcttggga
    10261 aggaaacggt tgcaaccaga ctgcaggttt tgtcctcaac ctcccaatca aagaaaatat
    10321 gagaaatctg cgagttaaga ttgagaaaaa gacgggccta ctatggaata gatggcaaac
    10381 aatctatgaa aacagaccaa ttttagctca accccaccgt aaaattaccc attggggtac
    10441 gacattgaat tccaaggtga gtgacgatga tgtcttgtaa tctgatggta caatgacagt
    10501 tagtttgtct agtttataag aaagtactac ctgagcttga atagaactca ggtagctctc
    10561 tatgaaagaa caaaattaat actcaatgaa aatcaaagag caaactagga aactagccgc
    10621 aggttgctca aagcactgct ttgaggttgt agataagact gacgaagtca gtcacatata
    10681 taatccaagg cgacgttgac gtggtttgaa gagattttcg aagagtataa acagaaaggt
    10741 agagcgcgtg ttctaatttg aacacgagta gaaaactttt ctaaaaacaa aaacgaaagg
    10801 atgggtaaac tgtattcgct gaactgaata cgggcgactc tcctctaaat caaaattaag
    10861 aaagaaagga attgaaccac cctaaaagca gtgggaaaaa gatagttggt ctagcgagca
    10921 tcgctcactg cgcccaactc ctattttccc ttcgcttttt gacgggtttg gtatctttct
    10981 caatataaaa tataaaataa agaaaggtag agcgtgtgtt ttgatttgaa cacgagcgga
    11041 aaactcggaa aatagataat ctgactgaaa aatcaggatt tctcgtcagg ttcctaattt
    11101 tcagtcgttt tcttctcgct ctttgtatca taaattatgt ctatccatat tgctgctcag
    11161 cagggtgaaa ttgctgataa aattcttctt cctggggatc ctcttcgtgc taagtttatt
    11221 gcggagaatt tccttggtga tgctgtttgt tttaacgaag tgcgtaacat gtttggttac
    11281 actggtactt acaagggtca ccgtgtatct gtcatgggaa ctgggatggg aatgccatct
    11341 atttcgattt atgcgcgtga gttaatcgta gactacggtg tgaagaaatt gattcgtgtg
    11401 ggaactgcag gttctttgaa tgaagaggtt catgttcgtg aattagtttt ggcgcaggcg
    11461 gctgcaacca actcaaacat cgttcgtaat gactggccac agtacgattt tccacaaatt
    11521 gctagctttg atttgcttga taaagcctac catatcgcca aaaaacttgg tatgactact
    11581 cacgttggga acgttttgtc atctgatgtc ttttactcaa attactttga aaagaatatc
    11641 gagcttggta aatggggagt caaggctgtg gaaatggaag cagcagctct ttactatctt
    11701 gctgcccaat accatgttga tgcgctagct atcatgacca tctctgatag cttggtcaat
    11761 ccagacgaag acacaactgc agaagaacgt caaaatacct tcactgatat gatgaaggtt
    11821 ggtttggaaa ccttgattgc agaataatta tagccaaaaa ggaagccctt tatgaattgt
    11881 aggacttcct tttcttatcc agaaattgat ctagctctct ctgatttcga agaatagtga
    11941 ctttatgtga atattcttgg caaagttttt ggtaattttc tttttgagtt ttgctacgcc
    12001 catcccaaag aatccatctg ataaactccc actcaaagcg ttcagggcaa tctgccgcca
    12061 tactttctct gacttttcca cggtatttaa gataacgctt aaaggctcta aagagacagg
    12121 tcaatggcga aaaattgaga aagatgattt ggtcagcttc ttgcattcgt tcttggtagt
    12181 agcaccaaga ataattacca tcgatgaccc aagctttatg cttggtgaga aagtttttta
    12241 tctcggttaa catccattcg cagtcactgt cttgccaacc aggttgaaat tggagtgtgt
    12301 ccatgtgcag ttttggaatg gagtagtagt tagataactt ttctgctaga gttgacttac
    12361 cagaaccaga atatccgata attgcgattt tcattttcta ccttttccta tttggagaca
    12421 aaaaaacagc ctctatggac tgtttcttat ttagcaagtt tagctgaaag acgagcttta
    12481 tcgcggcttg ctttgttttt gtgaatcaaa cctttagttt ctgctttatc gatagctgag
    12541 ctagcagcac ggaaaagttc ttcagatggg tttgcttcga aagcttttat agcagtacgc
    12601 atagctgatt tttgagctga gttcttttcg ttttgtttaa cgttcaattc agcgcgtttg
    12661 atagctgatt taatgtttgc caatgttctt acctccatat ttactaacta taccattata
    12721 tatgaaaact tacgttttga caagggaaaa ttactttttt aagtaaattt catcgatttc
    12781 atggttcttg cttttatgaa gaatcacttc tgcacgattt ctggttggtt caatataatt
    12841 ttgcagattt gtgagattga tactggtcca gacctgatgg gcaaaggact ccacttcccc
    12901 aatcggcatc tgagtaaaac gataatagta gctatcaggg tcgttttggg ctaggctcag
    12961 cattttcaag aaacggtcca gataccaact ttcaatatca tccactccag catctacata
    13021 gatggaaaag tcaaagaagt cagtgatata gagacgatcg ttttgtggat tttgaaagac
    13081 attaattccc tcaacaatta caaaatcagc agctttgaca ctttgtttct tttcgggtac
    13141 gatgtcgtaa acttcatgag aatagacagg aatatctaca tcttgtccat ttttgatgcg
    13201 gtccaagaag ttgagaagag cttccatatc atagctttca ggaaatcctt tacgatttaa
    13261 aatcccctgc tcaatcaagg tttgattggg atagagaaaa ccatcagttg taaccaactc
    13321 aaccgtagca tctgtaaacg tacgggacag taggatttga agtaggcgac tggttgtgga
    13381 ttttccaacg gcaacactcc cagaaacccc aataataaaa ggttgagatt tactttcacg
    13441 ttggaggaaa attccttttg aaaaggctaa atcttcctta gttcgcttgt aaatctgaat
    13501 cagatgagcc aaggggagat agatatctgt aacgtcttgg agactgattt ggtcattaaa
    13561 actcttgata gattccaatt cttcttctgt caaaggaggt gttgtctttc gatgtaaaga
    13621 ttgccaagtc tggcggctga ttttttcaaa atgtaaaaat tcgttggtca tttgttttcc
    13681 cctaggtatt ttatctatca taccataaaa agctaaaaaa ataaagcggt ttcttgaatg
    13741 ttgtaagtca atgatatata atataagcga tcaatttatt gatgaaatag gagatagatt
    13801 ctactgattt ataagcttat aaagggggct ttttagttca caacttatag gagatgtact
    13861 atgaaaatct taaaacgtta catattggaa ctctgtttta ttttaagttt tgctttacct
    13921 tttataaaag gagccaatgc agataatggt agatgctttg tggaaaccta ttacggtttt
    13981 acttttttga tggaacatgc tattgtaaca gctgtcttta tctgttcgtt cttaattgct
    14041 gtcttactaa aaaacgatgg gcgaaatgga ttgcgacaga gggttatttc ttccgcatgt
    14101 cactagaaga tttgatacga ctttggacaa gtttggaaat cctgacacaa acgtatcagt
    14161 tgggctttta tctaaacatt ttgttaggaa ttttgttgat aataaagtat tttaaggtta
    14221 agcaatagaa ata
//
LOCUS       DAVKZB010000042        14194 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKZB010000042 DAVKZB010000000
VERSION     DAVKZB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.042"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..174
                     /locus_tag="VXA80_001128"
     CDS             <1..174
                     /locus_tag="VXA80_001128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353632.1"
                     /translation="DCSKHRFKVVDRTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWF
                     EEIFEEYHSPFHN"
     gene            complement(508..1644)
                     /gene="dnaJ"
                     /locus_tag="VXA80_001129"
     CDS             complement(508..1644)
                     /gene="dnaJ"
                     /locus_tag="VXA80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV0353633.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(2046..2402)
                     /locus_tag="VXA80_001130"
     CDS             complement(2046..2402)
                     /locus_tag="VXA80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV0353634.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(2404..4227)
                     /gene="dnaK"
                     /locus_tag="VXA80_001131"
     CDS             complement(2404..4227)
                     /gene="dnaK"
                     /locus_tag="VXA80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV0353635.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4696..5220)
                     /gene="grpE"
                     /locus_tag="VXA80_001132"
     CDS             complement(4696..5220)
                     /gene="grpE"
                     /locus_tag="VXA80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV0353636.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(5247..6281)
                     /gene="hrcA"
                     /locus_tag="VXA80_001133"
     CDS             complement(5247..6281)
                     /gene="hrcA"
                     /locus_tag="VXA80_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV0353637.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6445..6954)
                     /locus_tag="VXA80_001134"
     CDS             complement(6445..6954)
                     /locus_tag="VXA80_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV0353638.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREELSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7562..9895
                     /locus_tag="VXA80_001135"
     CDS             7562..9895
                     /locus_tag="VXA80_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0353639.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9908..11371
                     /locus_tag="VXA80_001136"
     CDS             9908..11371
                     /locus_tag="VXA80_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0353640.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVCDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            11371..12954
                     /locus_tag="VXA80_001137"
     CDS             11371..12954
                     /locus_tag="VXA80_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0353641.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKKFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFS
                     IDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHV
                     TVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPE
                     QSRIVDKIEQFFAHIDALI"
     gene            13011..13808
                     /gene="psrA"
                     /locus_tag="VXA80_001138"
     CDS             13011..13808
                     /gene="psrA"
                     /locus_tag="VXA80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV0353642.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            13819..>14194
                     /locus_tag="VXA80_001139"
     CDS             13819..>14194
                     /locus_tag="VXA80_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0353643.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEA"
BASE COUNT         4478 a         2651 c         2724 g         4341 t
ORIGIN      
        1 gactgctcaa aacaccgttt taaggttgtg gatagaactg acgaagtcag ctcaaaacac
       61 tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacccatga caagccgacg
      121 ctgacgtggt ttgaagaaat ttttgaagag tatcattcac catttcacaa ctaaatcgaa
      181 gactttctgc cgtaatgaaa acacctgaaa cagcttggtt tcggctgtcc ggaaacttta
      241 agacttaggt tcaaagttta ggtatggaac ttcgaaggag gtcgctaccg tccgtcatta
      301 cttaggaaag tcttaaaaaa tctataaagt aaaaagagcc cgacagctct ttttgtttat
      361 actcaatgaa aatcaaaaag caaactagga aactagccac gggctgctca aaacactgtt
      421 ttgaggttgt agatacgact gacgaagtca gtcacatata tacggcaagg caacgctgac
      481 gtggtttgaa gagattttcg aagagtatta ttctccatca aaggcatctt taatatggtc
      541 aaagaagcct tttttctttg gatttacttt caagtcacca gcagccgcga attctttcaa
      601 ggctactttt tggcggtcgt tcaagcctgt cggtgttacg acattaacag taacgtattg
      661 gtcaccaact gcaccgccac gaaggctcgg tgccccctta ctacgtaggc ggaacttctt
      721 accagtctga gttccctctg gaataaccaa ttcaacatca ccgtgaacag ttggaatatc
      781 tactgtatca ccaagagccg cttggacaaa gttgaggttg agattgtaga agatagtcgt
      841 tccttcacgt tcaaacttgt cgctagcttc cacagaaact actacataca agtcaccata
      901 aggtccaccg ttaaagcctg cttcaccttg accagcgagg cgaatttgtt gacctgtttc
      961 cacaccagca gggattttca catgtacgct atgagcttgt ttctcatgac ctgttccatg
     1021 acaggttgta catggatatt tgatttcttt tcctcgaccg tgacagacat cacaggttac
     1081 ttggcgacgc atcataccaa gaggagtctg cgtatcgacg ttaatgacac cagcgccatg
     1141 acagcgtcca caagtgactg gacttgtccc tggcttagca ccagatccat tacatgtacg
     1201 acagccagct tcacgatgat acttaacttc cttctcagtt ccgaagatag cttcttcaaa
     1261 ggtcaaattg acacgatact ggagatcatc tccttggcga ggagcgtttg gattgcgcga
     1321 agaaccgcct ccgccgaaga aacttgagaa aatatcctca aaaccaccga agccacctgc
     1381 cccattgaaa ccgccgaaac caccagctcc accaaaacca ccattggcgc ctgcagcacc
     1441 atactggtca taggcagcac gtttttggtc gtcactcaaa gtctcatagg cttcttgaac
     1501 ttccttgtac ttgtcctcag caccaggctc cttgttgata tctgggtgat attttttgga
     1561 aagcttacga taagcctttt tgatttcgtc tgccgaagcg tttttggata cccccagacg
     1621 atcataaaat tcagtattgt tcataattca agatacaaag ggcgtcaaac ggacacagcc
     1681 atctaggagt tttgacgacg gacgcttacg tcctagaaaa aactatcttt ttggcacagt
     1741 ccatagcccg tgttcagtta cgaacaccct tattcctttc tattgatgtt ctgaatcgaa
     1801 ccaccattta ggtcgggttc aattcctttc ttttatattt agtaagaaat tctgaaaccc
     1861 gtgttcagtt acgaacactc tttgttcctt tctattgatg agacaaaacc caaacaaatg
     1921 agattccgtc cctttttatc gaaaaacaga ggctgagttg caacctctgc aaataagtaa
     1981 attaacttta ttttgatttt aaatatccta taagtacaga aatatttata tatttttgtc
     2041 tcttcttagt atttgcctat ataattccaa ttgatcataa gaaatacaac tactgtccaa
     2101 atcataccaa ttatgaacgg tattacaaat ttatatgttt gtatttctaa tgatatcata
     2161 gctgatacca tcgctgatac aaatgcagac aacaatactg aagtaacaaa attccttacc
     2221 gcttcatcat ttttgacaca actatcatgc tcagctaata taatttctct atatatagtc
     2281 aaacattcta ctggaatatt atctataaaa ccttttgctg cttcatagtt gtcagcttga
     2341 ttacaaaaat cattataaac ttttcgtgta ttttttagat actcttcatc caatacactc
     2401 atcttacttt tccgtaaact ctccgtctac gacgtcatcg cctgcgtttc ctgttgcttg
     2461 tgcgccttct gctccttctt gagcttgttg cgctgctgcg gcttgttcgt agagtttaac
     2521 agcaagtcct tgagcttttt cgttcaatgc ttcaagtttt gctttcatgt cgtccaagtt
     2581 gttgtcttct tgagctttct taaggtcatc aagggcagct tgggcagcgt cacgttctgc
     2641 gtcgaagcct ttaccttcag tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc
     2701 tacttcatta cgaaggtcta cttcttcttt acgtttctta tcggcttcag cgtttgcttc
     2761 tgcatctttc atcatgcggt cgatttcttc gtcagtcaaa cctgagttcg attggatgac
     2821 aatagtttgt tctttttgag ttccaaggtc tttggcctta acagacacga taccgttctt
     2881 gtcgatgtca aatgttactt cgatttgagg aattccacga ggtgcagctg ggatatcagt
     2941 caattggaag cgtccaagag tcttgttatc tgctgccatt gggcgttcac cttgaagaac
     3001 gtggatatca acggctggtt ggttgtctgc tgctgttgag aagacttgtg atttagatgt
     3061 tgggattgta gtgttgcgat cgataagttt tgtaaatact ccacccattg tttcgatacc
     3121 aagtgacaat ggcgttacat caagaaggac aacgtccttg acatcaccag taatcacacc
     3181 accttggata gccgcaccca tagcaactac ttcatcaggg tttactgatt tgtttggttc
     3241 tttaccagtt tcagctttaa cagcttcaac aacggcaggg atacgagttg aaccaccaac
     3301 aaggataact tcgtcgattt ctgacaagct caaacctgca tctgaaaggg cttgacgaac
     3361 tggaactttt gtacgttcaa caaggtcacg agtcaaatcg tcaaatttcg cacgagtcaa
     3421 agtcatttcc aagtgaagag gtccagcctc acctgcagtg ataaatggca agctgatttg
     3481 tgttgaagtt acaccagaaa ggtctttctt cgctttttca gccgcatctt tcaaacgttg
     3541 cattgccatc ttgtcagtag acaagtcgat accgttttct ttcttgaatt ctgctaccaa
     3601 gtggtcaatg attttttggt caaagtcgtc accaccaagt ttgttgtccc ctgcagttga
     3661 caatacgtcg aagacaccgt cacccaattc aaggatagag acgtcgaatg taccaccacc
     3721 aaggtcaaat accaagattt tttcttcttt gtcagtcttg tccaaaccat aagcaagagc
     3781 tgctgcagtt ggttcgttaa caatacgttc tacttcaaga ccagcaattt taccagcgtc
     3841 ttttgttgct tgacgttgag cgtcgttgaa gtaagccgga actgtgataa cagctttggt
     3901 tactttctca ccaaggtagt cttcagcgta gcctttcaag tattgaagga tcatagctga
     3961 gatttcttgt ggagtgtatt cttttccatt tgcagaaact ttttcagaag ttcccatctt
     4021 agatttgata gagataactg tatctgggtt tgtaactgct tgacgttttg cagcatcacc
     4081 aacgatgatt tctccgtttt tgaatgagac tacagatgga gttgtgcggt ttccttctgg
     4141 gtttgcgatg attttgcttt cagttccttc aagaactgca actgctgagt ttgttgtacc
     4201 taagtcaata ccgataattt tagacatgtg tttttctcct tgttagttaa ttttctattt
     4261 ttctttttga tactcttctt aagtggcgac gccgtcagag cttgacttcg tcagtctctt
     4321 ttactaaact ttgagcctaa ggtctcaaaa tttgcgcaaa tagcgccacg gcgaagagta
     4381 tcgtctttgg ttcgcttcgc tcactattac aaagccaaac atattttatc tttcttcata
     4441 gtttattgtc gtttcggaca agttttctat tatgtaaatt gcgacacgag gagtcgaaat
     4501 cgattttatt tcgacgacga gttagtaagg aagctaggca aacgccatag caattgccgt
     4561 tttctgacga gttgctttag ctaccgtcag aattgccgaa tcgaacacgc cctaagctct
     4621 gagtaaaaaa gatagatttt cttgtgttca tcgaacactt cgtcaatctc ctatttttac
     4681 tgcgagcttt gtatcttagt tatacaccac taccattgct gggcgtagga tgcggtcatg
     4741 gagtttgtag cctttttgaa agacttgggc gatggtatct actgggtgtt catcgtctgc
     4801 tgggagagtt tggatggcca tatggtagtt atggtcaaat tcgccatccg ctgcgatttc
     4861 ttcaattcct tcttctttca aagcgtgaat caagctttct tgcaccatcc ccaagccctt
     4921 cttcacatca tctgtcaaac cttcaactgc aagtgcacgc tcaaggttgt caagagatgg
     4981 taagattgct tttgccaagt cctggctacg ataacgttgc aagttttgac gttcttcatt
     5041 ggcacggcgt tggatatttt gcatttctgc atgagcgcga agatatttgt tttcgaactc
     5101 atctgcacgt tcatttgcca agtccaactc agacttttca ggagttgttt cttcagctgt
     5161 ttccacaact tcctcttctt gaacttcttc tacttcttca ttttttatat cttgggccat
     5221 gttcgcctcc tttaatgatt tcaatcttaa tgtacttcat aatgattact gctgaggtag
     5281 cggtaaaaat ctgtcaactt catggtcaaa acacggttga ccacattgat ttgattgatt
     5341 agctgttggt aatccagatt aactggaccg ataatggcta gaattccaac tccccgataa
     5401 ggaatgagga acttactact aatcaccgct aggtcagcta aacaggactc ttgaccgtct
     5461 gcaacacgaa cattttgcat ctgatcctca cgcaacccct cacgaatctc caaggccacc
     5521 ttttgcggtt ggtcaaagaa ctgataggct gctagattgg caaaattcaa gagatggacc
     5581 ttgcccgcca tcacaatgtt ttcgttgaac atttccttaa agatgtgttc aaagagatcg
     5641 atgacattat ccgttgttgt aaagtaacgc tggataatct gcggaatctc cgtccgaatc
     5701 ttgtagtgaa tatctaaaac ggtgtgaccg aggaaacgtt cctgaatgat gctcttcagt
     5761 ttcagcaaat cctcctgcaa gaagttcctt ggaatcagaa actgactagt aaccgttcgc
     5821 gactcgtcta gggtaaatac cgccaaggct gtatgttgcc ccaaaacaac gatatcaaag
     5881 gctgtcaacc gttgcctgct cggctcaaca tccagtgcca ctaccgtaca gccactcagg
     5941 tctgttagca agttagcagc ctcttgcaga atatcctcca atttgaagaa ttcctgatca
     6001 aaggctttga caatctcata tacctcattt tcagccagtc ggtcaaaatc cagtgagtgt
     6061 ttcacatagt actgaaaacc agcaacactt ggcatccgac cgcttgaagt atgagccttc
     6121 tcaagcaacc cttgtttttc tagttccgcc atgtcattac gaatggttgc actgctagag
     6181 ttaatagact cttgcaaggc ttttgatccg acaggttcgt gcgttttggt aaagatgtca
     6241 ataatcagat ttaaaatatc ctgctgacgc tctgtaacca tctcatcact cctctctgtc
     6301 tgatcaatta gcactcaatg catccaagtg ctaactcatg attctattat acacccttag
     6361 aagagaatgt caagacaaaa actcaaaaaa ttagcactct tttatcaaga gtgctaaaaa
     6421 tcaatctgaa gacctagaac cttgctattc atctactcgg tatttccttt ttagtctgaa
     6481 ataaccatga attagataag aaatcagaaa agcatataaa acaaaaataa gaaagaaaga
     6541 catagaaagt tcctctctaa acaaactcat acaaacaaca agaccaccta agaaagtagc
     6601 tgtacaggaa cgaagtctag atcgcataac ttcccatttg agccccttac tcatatagac
     6661 ttgatcctct ggaagtaaat cagaagacga tttacgaaga atcgaacaag aacctgctcc
     6721 tatcaattcc caacctctat ctctaaaatc ttgcagttca tgcttatatt ttttaagaaa
     6781 tctagaatca tagatacggt agatgacatc gtctggttga cattggtcaa aatagaacaa
     6841 accaaaacga ctcgttctat acctccaacc tttcaaatgc atctcatgta aatattcttc
     6901 ttccttgtcc aaatcaacaa tagtgaaaat ccgaaattct actctgctat tcattgtctt
     6961 accccaaaat tagaaaacat gcctggcgtt atttattaga taattctttc cacttttgac
     7021 tcaatctcca aaaaatataa gaaatctgaa tcgcaaaaac tatcaataaa acccaatcta
     7081 ttatgaaagt caaaaacact tcccaactga aagaactacc tccagtgaca aactttgaga
     7141 aaaacggtag tagagctaaa aagagaagca aaataggaag catccgcatt gttaaaatcc
     7201 ttttggcata aaaaaatctt tatttaaact aaaatattat ggtaaaattt acgccagttt
     7261 ttgaacggct gatgtagata ttttatactt tcaaaatgtt taaatgtgat tatttatttt
     7321 tgaaaaatag ctcaccagcc cgactgaaag tgcttataga atgataataa gtcgcctgcc
     7381 gaaaacagcg gaaaatagcg gtgttatgcg gagataatct gacgcgatgc gaaagtatat
     7441 tgcatactta ttttcaacaa tttagcagag tatttttata agtgtgatat aatagaagta
     7501 taatttgttc tgatagttta ttttatggag aagtagattt ttagaatgcg gaggattcaa
     7561 tatggttgag tttataaagt ctaagaaaga aatgagtgag gaggatatta aagcaaattt
     7621 catcactcct gctattgtat ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga
     7681 atacttcact gatggtcgaa ttgaggttag aggagataag gctcgtcgta aagaaggaaa
     7741 aaaatcagac tattcactgt attaccaatt tggaactcga attgcaattg ttgaggcaaa
     7801 ggataataaa cacagcgttc gagcaggatt acaacaagct attgaatatg gagagatttt
     7861 agatgttcca tttgtttatt cttcgaatgg tgatggcttt attgaacacg accgtatcac
     7921 gagagaagaa cgtgagctgg agttagacga attccctact cgtgaagaat tattttctcg
     7981 tatgacgaag gaaaaaggat tgacgtacga aattacagaa gctatctcaa ctccatacta
     8041 tacagacgcc ttctcaatga aaacgccacg ctattatcag caaatagcta tcaaccgtac
     8101 tattgaaaca gttgccagag gacaaaaacg agtaatgttt gtgatggcaa caggaacggg
     8161 gaaaacgttc atggcttttc aaattattca tcgccttcga aaagctggtt tggctaaacg
     8221 agttttattc ttagcggata gaaacatctt agtagaccaa acgatggctg aagactttaa
     8281 gccattcgaa aaggtaatga cgaaaattac accaaaactt ttgactgctc ctgaaaaatt
     8341 aaattctttt gaaatttatc tagggcttta tcagcaacta actggtgaag atggaactga
     8401 aacacattat caaaaatttg acaaagactt ctttgattta atcgtaattg atgaagcgca
     8461 ccgtggttca gctaaggaaa acagtaactg gcgtaagata attgattatt tcagttctgc
     8521 gacacagatt gggatgaccg ctactcctaa agaaaccaag aatgcttcca atacggaata
     8581 ctttggtgag ccaatctata cttatagttt aaaacaggga atcgaggatg gttttttggc
     8641 tccatatcgt gttatgaggg ttaatttaga tgtggatgtg gatggttatc gtccagaaac
     8701 tggaaaagtt gatgctaacg gacaattaat agaagatagg tactacggca ggaaagattt
     8761 tgataaaacc attgtcattg atgatagaac gcaaagagtt gccaagtttg tttctgatta
     8821 tatgaagcaa aacaatgcac gatttgataa aacaattgtt ttttgtgttg atattgacca
     8881 tgccgagcga atgcgtgctg cacttgtaaa agagaatcta gacttagtcc aagaagacta
     8941 tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga aaagctcaac tggataactt
     9001 tatggatgtc aattctaatt ttcccgctat tgtaacaacg tctaaattat taacgacagg
     9061 agttaatgct aaaacatgtc gtttgattgt tttagactct aatatccaat ccatgactga
     9121 atttaaacaa attattggtc gtggcacacg tctttatcct caaaagggga aagaattttt
     9181 tacgattatt gattttcgaa atgttaccaa tttgtttgct gaccctgatt ttgatggtga
     9241 tccagtgaag gtgctggaaa caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt
     9301 agatgaggaa ggtgacccag tagaaaaata tatcgttaca gacaagcagg ttaccattct
     9361 taattctact gttcaagtat tggatgaaaa cgggaaactg attaccgaaa gcctgaccga
     9421 ctacactcgg aagaatatct taggtagcta cgccactttg aacgatttta tcacagtttg
     9481 gcatacggcg gataaaaaga agcttatctt agacgaactt tataaaaaag gagtttatct
     9541 agatgctatt cgagagtcgg agggaatatc agaacaagaa atcgatgatt ttgatttact
     9601 cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg gaacgtatca ataaactcaa
     9661 acaaagcgga tatttatata aatatagtga ggaagcgcgt gctgttttgg aaattttact
     9721 gaacaaatac atggataaag gtattggaga actcgaaagc attgaaacat taaaacttcc
     9781 agaatttcag atatatggtg gaaccttcaa aatcatcaat acttattttg gagataaaaa
     9841 acgatattta caagcaatta aagaattgga gcaagagcta tttacagtag cttaatgaaa
     9901 ggaaagtatg tcaattacat catttgtaaa aagaattcaa gatatcactc gaaacgatgc
     9961 tggtgttaat ggtgatgctc aacgtattga gcaaatgtct tggttattat tcttaaaaat
    10021 ttatgatagc cgtgaaatgg tttgggaatt agaagaagac gagtatgagt caattatccc
    10081 agaggaatta aaatggcgaa attgggctca tgctcaaaat ggggaacggg tattgacagg
    10141 cgatgaatta cttgattttg tcaataacaa gttattcaaa gagttgaaag agcttgaaat
    10201 aacttcaaat atgcctattc gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa
    10261 ctatatgaaa aatggcgtct tgttacgcca agtcatcaat gttattgatg aagttgattt
    10321 caatagccct gaagatcgtc attcgtttaa tgatatttac gaaaaaattc ttaaagatat
    10381 tcaaaatgct gggaactcag gagaatttta tacgccacgt gcagcgactg attttattgc
    10441 cgaagttctt gacccaaaac ttggagaatc aatggcagac cttgcttgcg gaacaggagg
    10501 cttcttgact tcgactctga accgtttaag tagtcaacgt aaaactagtg aagataccaa
    10561 aaaatataat acagctgttt ttggtattga aaagaaagca tttcctcatc ttttagcagt
    10621 tacaaatctg tttcttcacg aaattgatga ccctaaaatt gttcatggaa atactttgga
    10681 gaaaaatgtt cgtgaatata cggatgatga aaaatttgac attattatga tgaatccacc
    10741 ttttggaggg tcagaattag aaacaataaa aaataacttt ccagcagaat tacggagttc
    10801 tgaaacagct gatttattta tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt
    10861 tggagttatt ttacctgatg gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca
    10921 aaaactggta gatgagttca acttacatac gattattagg ttgcctcata gtgtctttgc
    10981 accgtataca ggaatccata cgaacattct tttctttgat aaaacaaaga aaacagaaga
    11041 aacttggttt tatcgtttag atatgccaga tggttataaa aatttctcga aaactaagcc
    11101 gatgaagtca gaacacttca atcctgtttg tgactggtgg gaaaatcgtg aagagattct
    11161 ggaaggtaag ttctacaaat ctaaatcatt tacacctagt gaattggctg agttgaatta
    11221 taatttagac cagtgtggct ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt
    11281 gattcagaat tatcaagcgg aaagagcaac tttaaatcat aagattgata atgtattagc
    11341 tgatattttg cagttgttgg aggacaaata atgacaccag aacaacttaa agcaagtatt
    11401 ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt
    11461 gaattattaa agagaattaa agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa
    11521 cgagataaaa aggaaactga gatatttcgt ggtgatgatg ggaaacatta tgggaagttt
    11581 gctgatggaa gtactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg
    11641 gtgaggtttt ctacattggt tgaaattgtc agaggtggct ctccacgacc aatcaaagat
    11701 tatcttactt ctgaagtaga tggaataaat tggataaaaa taggtgatac tgaaaagggt
    11761 gaaaagtata taaataatgt taaagaaaaa atcaaaaaat cagggcttaa caaaactaga
    11821 tttgtaaaaa aaggtacatt tttgttaact aattctatga gttttggtag accttatatt
    11881 ttgaatgttg atggtgcaat acacgatgga tggttggcta tttcgaacta tgaaaactca
    11941 ttaaataaag attacctatt ctatattctt tcatcaaatg tagtttattc tcaatttcta
    12001 tctctaatta gtggagctgt tgtgaaaaac ttgaatagtg ataaagttgc ttctattctt
    12061 atccctctcc ctccgctagc tgaacaacaa cgcataatag aagcaatcga atcagcttta
    12121 gaaaaagtag atgaatatgc tgaaagttat aatagactag aacagctaga taaaaaattt
    12181 ccagataaac taaaaaaatc tattcttcaa tatgctatgc aaggaaaatt agttgaacaa
    12241 gacccaaatg atgaatcagt cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa
    12301 ctctttgaag aaggcaagat taaaaagaaa gatttggaca tttctattgt ttcccaagga
    12361 gatgataact cttattatga ggaagtacct tgtgaaatac cagaaagtga aataccagaa
    12421 agttgggaat gggtgaggtt aaacgatata acctcctaca ttcaaagagg gaagtctcca
    12481 aaatattcaa atattcctat ataccctgtg attgctcaga aatgtaatca atggtctggc
    12541 ttttctattg acttagcaag atttattgat cccgaaacag ttcatagtta ccagaaagaa
    12601 agattactaa gagatggtga tttaatgtgg aattcaacag gtctaggtac gttaggacga
    12661 ttagctattt accatgaaaa taaaaatcct tatggctggg ctgttgcaga tagccatgtt
    12721 acagttataa gagtcttatc gggagttatt aattgccatt tcatctataa ttttctttcc
    12781 tctcctattg ttcagtcagt tattgaagaa aaagcgtcgg gtagtacaaa acaaaaagaa
    12841 ttattaacga aaactataaa agaatattta atccccctcc caccccttcc tgaacaatcc
    12901 agaatcgttg ataaaatcga acaatttttc gcccatattg acgcactaat ttagtataat
    12961 agttttacgt gcggtggaat ttctatcaaa cataagatag gagttttacg ttgaaagatt
    13021 ttgaattaca tttgaagaaa gctggtttag ctgaaaatac tgtgcgttcg tacctttatg
    13081 gtgttcgatt tttcttggag aattacgaac tcaaaatgga agatttattt gaatacaaag
    13141 gatatttatt agacaatttt aaacctaaaa ctgtcaattt gcgactacaa ggagtaaata
    13201 aatatctggc ttttattggt catgatgatt tgaaattaaa atttgtcaaa gtacagcaaa
    13261 aaccgttttt agaagatgtc attagccatg ctgattacct ttttcttaaa cgtagtttga
    13321 aaaaagatgg tattttaaaa tggcattttg tcgtttggtt tttaggtgca actggggctc
    13381 gtgtaagtga acttattaaa ctaaaagtag agcatgtaga aatcggttat tttgacattt
    13441 actcaaaagg tggtaaaatt cgtagactgt acattcctaa aaaattaaga aatagttgtc
    13501 ttagttggtt agaatcagaa aatcgccgaa gtggttattt atttttgaat aaatttaacg
    13561 aaccaattac agcaagagga gttgctcaac agttaaaaaa ttatgctgat aaatacaaaa
    13621 tgaatcctaa agtaatttac cctcattctt ttaggcattt atttgctaag aattttttag
    13681 cgaagtataa tgatattgcc ttgcttgcag atttgatggg acacgaaagt atagaaacta
    13741 ctcgaattta tctaaggaaa acagctactg aacaacaaaa tattgtagat aaaattgtta
    13801 attggtaaaa aataacaggt ggtcaaactg actacctgct atttttgtga ttatggctct
    13861 tattatggga ataaagacga aacaacctct tatcctatat atgaaatccc agaagcatgg
    13921 agatatatta aatttgctag cctagttaac ttcagaatag gaaaaactcc tccacgcagt
    13981 gaagctactt tttggggaac tgaaatacct tgggtatcta tatcagatat gcctatatct
    14041 ggttatgtaa ctaatacgag agaatctatt tctaaattag cattaaaatc aaagaaaata
    14101 gacatttcac ctaaaggaac tttattaatg agttttaaat tatctattgg aaaagttgcc
    14161 atattggata ttcctgccac tcataatgaa gcca
//
LOCUS       DAVKZB010000043        13434 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKZB010000043 DAVKZB010000000
VERSION     DAVKZB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.043"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(224..1831)
                     /locus_tag="VXA80_001140"
     CDS             complement(224..1831)
                     /locus_tag="VXA80_001140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV0353644.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2221..2808)
                     /locus_tag="VXA80_001141"
     CDS             complement(2221..2808)
                     /locus_tag="VXA80_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV0353645.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2936..3385)
                     /locus_tag="VXA80_001142"
     CDS             complement(2936..3385)
                     /locus_tag="VXA80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353646.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3390..3779)
                     /locus_tag="VXA80_001143"
     CDS             complement(3390..3779)
                     /locus_tag="VXA80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV0353647.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3807..4433)
                     /locus_tag="VXA80_001144"
     CDS             complement(3807..4433)
                     /locus_tag="VXA80_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0353648.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4447..5010)
                     /locus_tag="VXA80_001145"
     CDS             complement(4447..5010)
                     /locus_tag="VXA80_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0353649.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5175..5409)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5463..5966)
                     /locus_tag="VXA80_001146"
     CDS             complement(5463..5966)
                     /locus_tag="VXA80_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV0353650.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLSEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6159..6989)
                     /locus_tag="VXA80_001147"
     CDS             complement(6159..6989)
                     /locus_tag="VXA80_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0353651.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6974..8656)
                     /locus_tag="VXA80_001148"
     CDS             complement(6974..8656)
                     /locus_tag="VXA80_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353652.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKKEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8767..9015)
                     /locus_tag="VXA80_001149"
     CDS             complement(8767..9015)
                     /locus_tag="VXA80_001149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353653.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVR"
     gene            complement(9022..9570)
                     /locus_tag="VXA80_001150"
     CDS             complement(9022..9570)
                     /locus_tag="VXA80_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV0353654.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9585..10433)
                     /locus_tag="VXA80_001151"
     CDS             complement(9585..10433)
                     /locus_tag="VXA80_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV0353655.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10621..11970
                     /gene="trkA"
                     /locus_tag="VXA80_001152"
     CDS             10621..11970
                     /gene="trkA"
                     /locus_tag="VXA80_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV0353656.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKEVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11974..13413
                     /locus_tag="VXA80_001153"
     CDS             11974..13413
                     /locus_tag="VXA80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0353657.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
BASE COUNT         3923 a         3060 c         2520 g         3931 t
ORIGIN      
        1 tcctaaaact ttgctagaaa ttgatttggc tttccgaatt aatttgttca gattttattt
       61 catttcaata tactgataaa tccattcact agcacagggc aagagggagt cgctattaat
      121 ttcaacggta gcaggtacat acagaggact cctacctcag atatactgca atatcattgt
      181 atgcaaaaaa cctctgagat ttttctcaaa ggttctgatt ttgctaattg ctgttctcaa
      241 ccgctgcagt aacaaaggca gtgtagagtt cttctggtcg gtttggacgg cttgacagtt
      301 cagggtgata ctgacaagct acaaagaatt tattttcagg aatttccacg atttctacca
      361 aacgattgtc tggagaaact cctgaaaaga caaagcctgc tgcctcaaac tgctcacgga
      421 aggcgttatt aaactcataa cggtgacggt gacggcgttg caccacttct tgattgtgat
      481 aagcagcagc agccttagag ccacgtttca acttagacgg ataaagtccc aaacgaaggg
      541 ttccacccat atcctcaata tcaatctgat cacgcatgat atcaatgata gggtattttg
      601 tttctggtgc aagctctgca gaattggcac cttcaagacc taaaacgtga cgagcaaact
      661 cgatacatgt caactgcatt cccaagcaga ctcccaacat tggaacatca ttttcacgcg
      721 catagcggat ggcttggatt ttcccttctg taccacgttg accaaaacca cctggtacga
      781 tgatcccgtc tgcatcagac aagagttctg ctacattctc tgctgtcaca tcattggcat
      841 tgacccaatt gattttaact tctgcatcat tgacatagcc agagtgtttc aaggcttcga
      901 ccactgagat ataggcatct tgcaactcca catacttacc aacaagggaa atcttaactt
      961 gtttcttgag gttcatgacc ttgtccacca tggctgacca ttctgtcata tccgctgctg
     1021 gtgcgtctaa tttcaaatga tcacaaacaa tttggtccat cccttgtgcc tgcaagttca
     1081 gtggaatttg gtaaagatgt tcaacatcca acgattcgat aacggcttct ggtgccacat
     1141 cacagaactg ggccagttta tttttaattc cttgaccagc tggctcttct gtacgaataa
     1201 ccaacatatt tggttggatt cccaagccac gcaattcttt gacagagtgt tgggttggtt
     1261 tggttttcat ttcaccagca gccttgaggt aaggaagcaa ggttgtatgg atatacatga
     1321 cattatccgc acccacatct gccttcatct gacgaagagc ctctaggaat ggcaaggact
     1381 cgatatctcc tactgttcca ccaacctctg tgataatgac atcagagtcg gtcgttagag
     1441 cggcacgctt gattttttct ttcaaagcat ctgtgatatg aggaatgact tgaacagttg
     1501 ccccaaggta ttctccacgg cgttctttac gaagaacttc actgtaaatt ttcccagttg
     1561 tcacgttgga atatttgttg agattgatat cgataaaacg ttcatagtga cccaagtcca
     1621 aatctgtctc agctccgtca tctgtcacaa aaacttcccc gtgctggtaa ggactcatgg
     1681 ttcccggatc aatattgata taagggtcaa acttttgaat ggttactttg agaccacgat
     1741 ttttcaagag acggcctaga ctcgctgcca caatcccttt cccaatggac gataccacac
     1801 caccagttac aaaaatatat ttcgtagaca tagattcctc tttctaaaat gctcaaggtc
     1861 ttgttaacta cgaggggata tagaaaacaa gaccctacta ataagaataa tctgggacga
     1921 ctgcaccaga ttcacaacta atactcttcg aaaatctctt caaaccgcgt caacgtcgcc
     1981 ttgccgtaga tatgtgttcc tgactttgtc agtcttatct acaacctcaa aacagtgttt
     2041 tgagcagcct acggctagtt tcctagcttg ctctttgatt ttcattgagt ataaaataca
     2101 agaagataac ttcttgaaaa aacaaaaata gctccctaag aactagggag ccccgacctc
     2161 taaaagaggt gcccaaacaa tatgatacct aaaaatagga taattgtcaa tagctaactc
     2221 ttattcctcg ctggtttcag cgtcgtcatc tgaaaactcg tcgtcgtctt cgttgagatc
     2281 cacatcttct cccaagtcat ctggagcgat ttcgttgatt tctgcatcat aagcttccac
     2341 ttcatttttt tcatcatctg gattttcatc atcgtatgaa agagctggat ctgcttcgta
     2401 tgcatcttcg tcttctggat catctgcatt gtagtcaatg gcatctgaat caccatccat
     2461 aaaggcattg acacgttttt tcttagcttt tggtgctact tcatcgtcgt cattttcttc
     2521 aagagcgatg atttcttcgt cgatttcgtc cacaccatac catgaacgaa gaccccattt
     2581 gttgtcccca agtgagatga agctaccgtc aaagttcaac tctgtgtaga acaaaggcaa
     2641 agcttcgcgg atatcgctgt ttgatgttcc aaggtagttt tgaatttcgt ttacaagatc
     2701 gctaaaatgc atctcatgat cgcgaccacg aagttccaag atagcacgcg ctacctcaat
     2761 catagatagt tcactttttt cttgcccagc aaatacttct aattccaaag cgtttctcct
     2821 catttatact actatcgcca gagcgaacag actctgacct cattttatca tttactcttt
     2881 attttacgat aattttgcgg aatagtcaaa ggttaagggg gagaaagtgg caggattaga
     2941 ctaattccaa tataaaactc cttccttttt ctgttgctcc attttccaca aatccaagcg
     3001 acttgaaaca cctcctagaa gcatgattgt aggtgtagat ttccttgact ctcaattctt
     3061 tccatccttt tactcgagcc aattcaatca aagcacttag aatctttttt ccaagtcctc
     3121 gatgttggta agcggaattc ccaatcacaa tggggagatt atcctgagat agtgtaatat
     3181 ccccaattgg aaaccattct cccttctcct tgacttcaat ccaaaaaagc tcaccatgcc
     3241 gatccaaata ggaatacatg gcttccaagg tcgcttgact gtaaggaagc ttcaccccat
     3301 ctacgaggta aaccaagttc acatcctgat accaagcaaa agcttcctca caatgattga
     3361 ccttataata aggaacaaaa cacaatgcac taccttttca acaaaagagt tgctgcttga
     3421 ttaaaaccat cacaccagtt ataccatttt gcttcatacc catcttgagc taggatacga
     3481 tcttctaaat caaaaacaga gtaaatcttt ctttcctcgc aagcttgcgc atagagatga
     3541 tatagttcat caccaccatc tctatcccac tcagcagaaa tcgtatcccg acctgccaat
     3601 aaagcctgat aagccctgtg atgcccatct gtaatcagca aacaatctcc aaaggcaaga
     3661 atactgattg gatcgacttg gtttgtttct gccgactggt aaagcatctg aatatcttgc
     3721 aacttctttt ctgataaata tagttgagtc ggatgaagat ctgctatatt gactttcatt
     3781 tctttctcct caagggaatt cgatactcac ttctgtttgc ctttaaatcg ccattggaag
     3841 cggagcttgt catagaaggg aaactcgata aacaggactc ccaagcccac acagagactg
     3901 gcaaagacgt ctgatgggta atgaaccccc agatagactc ttgataccag cacactgact
     3961 aggtagaggc caaggacgat ttgtacgatt tttctccaga ctggattttt aattcgttga
     4021 ctgagaatga caatcagagt acctaccatc aaagttacag ccagagaatg tccacttggg
     4081 aaggaaaatc ccttctcttc caccagatgt aagatagctg gtcgtgggcg ctggtagata
     4141 tttttaaagg tcacgattaa aagacctgcc aaagccagat ttcccagcat gaagaaactt
     4201 tctatcttcc atcgcttacg ataaaagaca aaagctgtaa tgacaaccca agtgataatc
     4261 actgggatat caatcagacg tgtgagggct cgaaaaagaa tagtcaaata atctggtaag
     4321 tctcctcgaa tggcagtctg aatcgattgg tcaaaattga ccaacatttc agggtaaaat
     4381 ttgaccatat agccaagaat aacgaaaagt aaaagggcaa aactgccctt cattaaaaat
     4441 gtttgtttat ctttcatagt gttttaaggt tggtttcaaa aggacgtaca acaaccagaa
     4501 tgaaacggaa aagattacac cctcaatcaa gttaaaaggt aataccatgg tcattaggta
     4561 gttggaaagt cccaaaattt ttccaatatc aaagttagca aacttagcgt acaaaggaac
     4621 agcataaaca tagttgagaa ccaacatggc caaggttaaa ccaacagttc cagctagaga
     4681 gcctagtagg aaacgaaggg ttgtccgttc ctttttccaa atcaaagcaa atacgatgac
     4741 aaaaactccc aaagctacga tattcatcgg caaaccaatg taagtattca ctccttggct
     4801 gttaagaagc aatttcaaga gtgagcgaag caagagcact cctagagcag caggcaaatc
     4861 catgaccacc agacccacaa ggactggcaa gatactaaat tcgatcttga ggaaagatgc
     4921 cgctggtaaa agcggaaagt caaagtacat cagcacaaat gagatggctg ataaaattgc
     4981 aatggtcgaa agtcgacgtg tgtttgtcat aacaggttcc tccaattttc tataaaatca
     5041 gaagaagttg gaaaggattc ctctatctat tctcactttt tatatcccaa aagttccctc
     5101 ttactctatt aaagaaaaac aaagcaagtg gttacaatcc ggctataaat ctatcaaaac
     5161 agacaaagct attctttcgt cttctcccat ccagactata ctgtcggttg tggaatctca
     5221 ccacatcagc ttgcgctcgc ggacttcttt aaagagaagg aaatagagat ttttctttta
     5281 gactaggcga caagctgtag gcataacttc tggttaggca aagcttgtca acaaagtata
     5341 aaaggaaaag atcatttcct agttaaagtc accgccggtc gggaatcaca ccctgccctg
     5401 aagactcttt tatcataaca aaaaacgctt gcaagcgcaa gcattttgtt cattttcatt
     5461 ttttatttca atttatccac ttcataggta tggacgagct caagacctgc aaagttctgc
     5521 tggcgaagag cctcatagac aatcatgcag acggtattag acacattgag actgcggaca
     5581 tgttcatcat tcatgggaat acggagagcc ttctcaggat gttctcgcat aaaatcttca
     5641 gataagccct tgtcttcacg tccaaagaga aaataatggt cctcgtcagt cgataaatcc
     5701 acctcagaat agactttttc cgcgaatttc gaaatcagat agagttttcc cttcatctga
     5761 gatatgaaat cttccaaact ctcgtaaaaa taaatctcta gcttatccca gtaatccaat
     5821 ccagcccgct tcatcttgcg gtcatcaata ggaaagccca taggcttgat gatgtggagg
     5881 ggagaattgg tcgcagcgca agtacgcgcg atattgcccg tattttgtgg aatttgaggt
     5941 tcaaataata caatgtgatt tgtcatgact tgcttccttt caccattgca aaaaaatagc
     6001 cacactgccc ggagtcaagc tcagcaaaca gcgtggttaa ggcatcgtta acttacctca
     6061 caacaggttt gaagtaaatc agcgaaacta ctttcttagt ataacacttt cagaatcatt
     6121 gtcaatagaa atgacttgat tttttcaatt ttttcaagct atttccaagg gttgtaaaat
     6181 cgtccctgat tctgcaagat aagtagtaaa ctaactacta aaaacaaggt tgccaagagc
     6241 aaggtaatat agtctccttt tttcaaggcc tgataactat accatgtgcg ttttttctct
     6301 ttcccaaagc ggcgaagctc catggcagtc gcaatggtat caatgcgttc tagcgagcta
     6361 aaaatcaagg gcgtaataat gagcagattg cctttgattc gttgcataag agaagctttc
     6421 ttggataatt ccatcccacg cgcctcctga gacatcttga tagtaaagaa ttcttcctgc
     6481 aaatctggaa tatagcgcaa ggtcaggctg acagaataag caatcttata gggcacacca
     6541 atttgattta aactggaagc aaactgacta ggatgggttg tcatcaaaaa gataatagcc
     6601 agaggaatgg tgcaaagata cttaatggcc aaatttagca gataaaagag ctcctggctg
     6661 gttagagtgt agacaccgat tccctgccaa atcacacttc tctctccata aagtccaacc
     6721 ccatactcgg gagaaaagag atagaccatc aaaacgttta aaacggcaaa taccgtcgca
     6781 aaaacggcta caaaggaaac atctttaaag cgaatttctg ataaatagag gagaaagact
     6841 gaaaagatgg caatcagcaa gagcattctg gtatcatagc taatcatggc cgccaatgat
     6901 accagaatga aaaagagaag tttcccagct cccgacaagc gatgaatcac agtatctcta
     6961 tgctggtaac cgattaattt agcttgcatc cctctctcct ttctttgtaa aatgccgtta
     7021 aatccagtgg atccacatct agtttcttag ccaagttaaa gatggaggtt tcttttagat
     7081 tggcttttac taacagctca ggatcgctca acagactggc tggaacagta tcggcaatca
     7141 attctccatc caccatgaca aggacccggt ctgaataatc cagcatcaat tgcatatcat
     7201 gggtaatcat gacaatggta tgcccttttt gatgtaactc ttcgagaaat tccataatct
     7261 cagtatagtt cttctgatct tgacctgcag tcggttcatc taggagaata atttcagctc
     7321 ctaagaccaa aattgaagca atggtgacac gttttttctg accaaatgac agggcagaaa
     7381 taggccaatt acggaattca taaagtccac agattttcaa ggtttcatag actctcgttt
     7441 caatttcctt ctcatccaca cctcgcaaac ggagccctag agccacctca tcaaaaatca
     7501 tattggttga aatcatttga ttaggatttt gtagcacata gcctactcgt tccgcccgct
     7561 ctgcaacaga atcgcctttt atatcctgtt tttcccaaag atagcgtcct tccgtctgaa
     7621 taaagctact tatagccttg gctagagttg atttccctgc tccatttttt ccgacaatag
     7681 caatcttttc acccttttta atatctaaat gtagggattt taaaatcggt ctatcatcat
     7741 aagagaaaga cacgtcctct aatttaaaga gtgactgcaa ttctggagtt tcttttgcca
     7801 gttcattctg cagctgaacc tgaccttttg agatagacat gttatccaga ttcgctaact
     7861 gttctttctt gactaagtcc acacctaatt gacggagagt cgttagataa aggggttctc
     7921 gaattccatt ttgagtcaat aaatcagttg caagcaactg gtcagggctc ccattaaaaa
     7981 ggatacgacc atcgtttatc aagacaatcc gatccacagg gcgatgcaga acatcctcca
     8041 aacggtgctc gataataaga gtcgtcgtcc cctcttcctt atgaatctgg tcaatcaatt
     8101 cgataatatc ctgacctgac ttgggatcta gattggcgag tggctcatca aacaagagaa
     8161 tcggactttc atcaatcaag acaccagcca gactgactcg ctgcttttgt ccacctgaca
     8221 aatcctgagg gcgctgatcc agtaaaggaa gaaggtccag cttttcagcc catttataaa
     8281 cacgaccttt catctcatct agggctgtca catcattttc cagagcaaac gccaaatctt
     8341 ctgccacaga caagccaata aactgcccat ctgtatcctg caaaactgtg ctaaccagat
     8401 gagacttatc atagatgctc atatcaaagg ctgtttgacc ctttatcaaa aattctccat
     8461 atgtctgacc cttgtaaata ttgggaataa tcccattcaa acactgaccc aaggtagatt
     8521 tacctgatcc agatggtcca acaattaaga ctttctctcc cttgtaaatg gtcaagtcta
     8581 tcccttgcaa ggtcggttct tgttgtgttt cataccggaa agagaaatcc ttccactcaa
     8641 ttatagcttc tttcatctta ctctcttcat tcgcttctta gacttctatt ttatcataaa
     8701 tcaagccctt cttgcagtct ctcctcttaa aatcttagcg ccaaaaagat tcctatccta
     8761 gcttacttac ctaactaatc tataaacatc gaaaaagact agttgcccag ccttccccat
     8821 cattttatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcgccttgc cgtaggtatg
     8881 gttactgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgacttcgtc
     8941 agttctatcc acaatttcaa aacactgttt tgagcaacct gcggctaact tcctagtttg
     9001 ctctttgatt ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgggttcc
     9061 tgcataggca agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc
     9121 cgcaacaatc ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc
     9181 aagtggtgcc aagacacccc aaacaagggc atttgcaagt agttgaatga ggttaaaaat
     9241 aagaatatct ttccagtcaa aaacaccatt gatcacgcga acgtactttc taaaaagtcc
     9301 cacaactaaa ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccagc
     9361 aagagagtcc ttgattgcat gaccaatcaa cccgacaagc aaaccgataa tcggtccaaa
     9421 aataatagaa agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg
     9481 ggttggaatg ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac
     9541 aacttgttta attgtaaatt taatttccat actattctcc tattttatcc ttctattttc
     9601 tttatttcaa tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct
     9661 tggttgatag ccaaacctaa acgatagaga gagttgatgt aaaggatggg ttgcccaatt
     9721 ctcacatctg caaatgattt gccatagaca acctgatttt gatagaccag catatcagca
     9781 tgatagatgg tcacttcaaa acgatcacca aattctggtt ccagcttgta aaattcttcc
     9841 cgtgtgatag aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc
     9901 accagatgat cttctatgat ggtcgctacg actggaagct ctacaatctg ttccacactg
     9961 agctctggcc ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtatag
    10021 gcatagacat cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc
    10081 acctcagaaa tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc
    10141 ccattatctg gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt
    10201 ttcgaaccga cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc
    10261 accgtctgaa agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc
    10321 aagtgatgga tttttaaggt tggagactct tctaaagcca ctccaatcat agccgacacc
    10381 gcaccatcaa ccagaccaaa gtctgattgt aataccagta aattattgtt cattttatct
    10441 ccttgtatat cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg
    10501 gttagggtat agttttacct tatagcgaat tttcactaaa agctcttgtt attagctagt
    10561 aggtgcattt tttcttgaaa aagggtatga tagttacatc atgaaaaagt aggttttatt
    10621 atgaaaatta tccttgtcgg agggggaaaa gttggttttg ccctctgtcg ctccttggtt
    10681 gcagaaaagc atgatgtttt gctgattgag caagacgaag ctgttctcaa tcatattgtc
    10741 agtcgctttg atatcattgg tatccttggt aacggggccg attttgccat tcttgagcaa
    10801 gccagcgtcc aagattgtga tatctttatc gccctgactg agcacgatga agtcaacatg
    10861 attgcagcag ttctagccaa gaaaatggga gctaaagaaa ctatcgttcg ggtgcggaac
    10921 cctgaatatt ctaactctta tttcaaggaa aagaatattc tcggtttttc tcttatcgtt
    10981 aatcctgagc tcttggctgc ccgtgctatc gcgaatatca ttgacttccc caacgccctg
    11041 tctgtcgaac gctttgctgg tggacgcgtt agcctcatgg aatttgtcgt caagtccacc
    11101 agcggtcttt gccaaatgcc catttctgat tttcgtaaaa aatttggtaa tgtcattgtc
    11161 tgtgcgatag agagggatca tcaaattatc attccaagcg gtgacatgac tgtacaggat
    11221 aaagatagaa tctttgtcac tggtaaccgt gtcgatatga tactcttcca taattatttt
    11281 aaatcacgcg ccgtgaagag ccttctcatc gttggggcag gtagaattgc ctattatcta
    11341 cttggtattc tcaaagatag tcgtatcgat acaaaagtca ttgaaatcaa tcctgaaatc
    11401 gccagcttct ttagcgagaa attcccaaat ctctacatcg ttcaaggaga tggaaccgca
    11461 aaagatatcc tgctggaaga aagtgctcaa cactatgatg ccgttgcgac tctaacagga
    11521 gtcgatgagg aaaatctgat tacatctatg ttccttgaca gggtaggtgt acagaaaaat
    11581 attactaagg tcaatcgtac cagtctcctc gagattatca atgcgcctga tttttcaagt
    11641 atcatcacac ctaaaagcat cgctgtagat acgattatgc actttattcg tggtcgagtt
    11701 aatgcccagt attcagacct tcaagccatg caccatctag ccaatggcca aatcgaaacc
    11761 ctgcaattcc atatcaagga agccaataaa atgactgcca aacctctttc tcaactgaaa
    11821 ttgaaaaaag aggttcttat tgcagccatt attcgaaagg gcaagactat tttcccaact
    11881 ggggaggata tgttggaagt tggagacaag ctcctagtaa caaccttgtt gccaaacatc
    11941 accaagattt atgacttgat tgcgaggtaa gaaatgaata aaagtatgat tcgttacctc
    12001 ctttcaaagt tacttttgat tgaagctgtt ctcctcttgg ttcctgtggc tatcgctgtc
    12061 tattaccgtg aatcgagcca agtctttaca gccctctttt caactatagg gattctcgta
    12121 ttgttaggcg gttcagggag tttacagaag ccaaaaaatc aacggattta tgccaaggag
    12181 ggagtcttga tcgttgccct ctgttggatc ctttggtctt tctttggtgg tctccccttt
    12241 gtcttttctg ggcaaatccc tagcgttatt gatgcctttt ttgaaattag ttctgggttt
    12301 acaactactg gagcaagtat tttgaacgac atttcggttc tcagccgttc cctcctcttc
    12361 tggcgaagtt ttacccactt gattggaggg atgggagtgc ttgtttttgc acttgctatt
    12421 atggacaatg ccaaaaatag tcacctagaa gtaatgaagg ctgaggttcc tggtcctgtc
    12481 tttggtaagg ttgtatccaa attaaaaaac actgcccaga tcctctatct cctttacata
    12541 gccctctttt ccctctttgt catcatctat tatctagccg gtatgcctct ctacgatagt
    12601 tttgtcattg ctatggggac agcgggaact ggaggattta ccgtctataa cgatggaatt
    12661 gcccactatg gtagctcact gattacctat ctggttagta taggagtttt ggtttttggg
    12721 gtaaatttca atctctacta ctaccttatg ctccgtcggg ttaaggcctt ctttggagat
    12781 gaagagcttc gggcttactt ggtcatcgtg ctggtttcta caggcttgat taccctcaat
    12841 actctctacc tctacccagg attttccaaa agctttgaaa tgaccttctt ccaggtttcc
    12901 aacatcatta caacaactgg ttttggatat ggagatatta ccaactggcc cctcttctcc
    12961 cagtttatcc tcctcttcct catgacaatc ggtggttctg ctggatcaac tgcaggtgga
    13021 ctcaagatta ttcgaggcct catcctttca aaaattgcca aaaaccaaat tttgtccatt
    13081 ctatctcccc accgtgtttt gactctccat gtgaataaaa cggtgattga taaggatacc
    13141 cagcataaaa ttctcaagta ctttgtcatc tatgctatga ttttgctagc gcttatcttt
    13201 attgtcagcc tagatagcaa tgattttctg gtcgtgacca gcgctgtctt tagctgtttc
    13261 aataatatcg ggcctattct aggaaccact tctagtttct caatctttag tcccatctca
    13321 aaaattctcc tatcctttgc aatgattgca gggcgcttgg agatttaccc aatcctcctt
    13381 ctctttatga agagaacttg gtctaagaga taaaatacaa aatccctctt ttta
//
LOCUS       DAVKZB010000044        13384 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKZB010000044 DAVKZB010000000
VERSION     DAVKZB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.044"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(134..1114)
                     /locus_tag="VXA80_001154"
     CDS             complement(134..1114)
                     /locus_tag="VXA80_001154"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV0353658.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1116..3368)
                     /gene="pbpX"
                     /locus_tag="VXA80_001155"
     CDS             complement(1116..3368)
                     /gene="pbpX"
                     /locus_tag="VXA80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV0353659.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(3372..3689)
                     /gene="ftsL"
                     /locus_tag="VXA80_001156"
     CDS             complement(3372..3689)
                     /gene="ftsL"
                     /locus_tag="VXA80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV0353660.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(3701..4651)
                     /gene="rsmH"
                     /locus_tag="VXA80_001157"
     CDS             complement(3701..4651)
                     /gene="rsmH"
                     /locus_tag="VXA80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV0353661.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            4817..5011
                     /locus_tag="VXA80_001158"
     CDS             4817..5011
                     /locus_tag="VXA80_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0353662.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            5027..5570
                     /locus_tag="VXA80_001159"
                     /pseudo
     CDS             5027..5570
                     /locus_tag="VXA80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
     gene            5581..5823
                     /locus_tag="VXA80_001160"
     CDS             5581..5823
                     /locus_tag="VXA80_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353663.1"
                     /translation="MKKEKRQLHYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            5834..6169
                     /locus_tag="VXA80_001161"
     CDS             5834..6169
                     /locus_tag="VXA80_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353664.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLTYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6279..7280)
                     /locus_tag="VXA80_001162"
     CDS             complement(6279..7280)
                     /locus_tag="VXA80_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0353665.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7340..9241)
                     /locus_tag="VXA80_001163"
     CDS             complement(7340..9241)
                     /locus_tag="VXA80_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV0353666.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFRPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEEELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPILATYHFFSDKMDLEEAYHFASKELKQQ
                     IETQILEDGSQFEQSILYHVEVYKALLDLCILLPDLQDSFQELLEKMATYIQMMTGLD
                     GRTLTFGDSDSTETTEILSLSAMVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFRSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREVDERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9263..9556)
                     /locus_tag="VXA80_001164"
     CDS             complement(9263..9556)
                     /locus_tag="VXA80_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0353667.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9556..10374)
                     /locus_tag="VXA80_001165"
     CDS             complement(9556..10374)
                     /locus_tag="VXA80_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0353668.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(10361..11140)
                     /locus_tag="VXA80_001166"
     CDS             complement(10361..11140)
                     /locus_tag="VXA80_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0353669.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            complement(11155..11646)
                     /locus_tag="VXA80_001167"
     CDS             complement(11155..11646)
                     /locus_tag="VXA80_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0353670.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(11657..12847)
                     /locus_tag="VXA80_001168"
     CDS             complement(11657..12847)
                     /locus_tag="VXA80_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV0353671.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(12859..13293)
                     /locus_tag="VXA80_001169"
     CDS             complement(12859..13293)
                     /locus_tag="VXA80_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353672.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         3942 a         3035 c         2364 g         4043 t
ORIGIN      
        1 agaagtagag gtgtactatt ctagtttcaa actactatgt tttgagcagc ctaaagttag
       61 ctttctagat tgttctttga ttttcattgt gtatttaaac acaaaaacac cctgaaacat
      121 cagggtgcca ttcttacatc aaatataaaa ttgcgagggt caggagactt gctagaagtc
      181 ccactcccca aaagaagaag tcaaccttcc actcgctcca aggatttcct ttaccagaca
      241 atcccccaag ctcaaaatgg tgatgtacag gcgtcatacg gaaaatacgt ttaccacctg
      301 tcagtttgaa ataactgact tgcatcataa cagaagttgt ttcaaaaaca tacacaattc
      361 cgataatcaa gagagtccat tcttggtgga gagccataga gatagctgcc agcatcccac
      421 ctagggccaa acttcccaca tcacccataa agaccttggc aggcttatgg ttaaagatga
      481 agaaaccgag caaaccacca atcatggcaa gaatcactag aagaatatcc atctgacctt
      541 gcacataggc aataactcca taggcagaca aactaatcac aacggaaata ctagctaaac
      601 cgtcaacacc gtctgtcaag tttactgcgt ttgaaaaacc gactagccag aaaagagcga
      661 agacaatata gaaaatccct agatgcactt gataaccaaa gacagaaagc atatcgccac
      721 cgcgctcata gaaaagatag aagataactc cacctagaag ctgaagagct aatttttgct
      781 taggattaag cccctcattg attttacgaa agaccttgag aaagtcatct aaaaatccga
      841 ccaagccata caagaccaag atgaacaaaa tcattcccac attattgctg aattggctac
      901 taaatagggc gaaaaagaaa gcaaccaaaa cagaagtaat caagaaaacc aaacctccca
      961 ttgtaggagt cccagctttt gcctgatgct gtttgacatc ctcatgcatc tgctggcctg
     1021 taatttgcgc ctttctataa aattggataa aggccggaat tcctactaaa gttagtaaaa
     1081 atgtcacaat tccagcactg agggaaataa acatattagt ctcctaaagt taatgtaatt
     1141 tttttaatgt ccttgatagc tgtgttagca cgaacatctt gcttctgcac agtagagccc
     1201 gaaccttgaa attcaagttc tatattgagc cacttagcaa aggtctcagc agtctccttt
     1261 gtccaaccat acatatctgg aacttcctct gctttatcag ataagataag gacttgctgg
     1321 ttcggggcaa gattcttccc ttcttcagca gaactgtttt taatcttcgt tcctgttccc
     1381 acaacgatgg gttgtacaag attgcgacgc aattcttctg ctaaatcacc aggtgaaata
     1441 tccttgacac taggcatagg ataaggactt tgttgactta cttgctccaa agctttagct
     1501 gttgtttgaa gattgagaga gtctttcata gctgaagccc gctccaagat aggattggca
     1561 aattctccca actgaatacc tgaataatgt tcaggttgtt ggaccgtcac atacaagata
     1621 aaatcaggat tttcagccgg actcatcgat acagccgaga aaatatagtc ggttaccccg
     1681 actagataac caccattttt ctcgtcagca atctgagccg taccagactt gagggctaca
     1741 ttttgcccag gaacagttac agttggcttg cctgtgctgt ggttatacat ggttccataa
     1801 accggatccg tccctaccaa aaccatgtta gtccgagtta gactagctgc atctttagaa
     1861 acaggatttc ccacaatttc tttttgagat ttccgagcag tttgatcatt tggatcataa
     1921 atggcactaa taaatttagg ctccagcatg acaccgtcat tagcaatagc tgtaaaggca
     1981 cgaatcattt gtgtctgggt cactgaaatc ccttgtccaa atgagctttg cgcaatgttg
     2041 acaatattat ccgcaggaag ctgaccagca tactcatccg tcaaaccgaa acgggtcgga
     2101 actccaaatt taaaacgatt aagataatca agccaggtag catctcccat cttttgctca
     2161 aggagagtca tcccaacgtt acttgagtgt gcaaaacctt gagaaaaagt catcattctg
     2221 ccaccagtca atccttcatt aacgtcccaa tctcgaatcg tggcatctgc aatttttaac
     2281 tcactactat taaagacttc tcctcctgga aaggtattat tatcaatagc agcagccaac
     2341 atcatcactt tcatagtgga acctggctca tagttacttt ggtaaaggat atcacgccaa
     2401 acaaagtcct ctgtaatgcc ttcttttgta tctgcatcaa aggtcggtcg ttgcgttgtt
     2461 gccagaattt cccctgtttt agcactgacc aaagtcgctg tcatgtactt tccttttacc
     2521 ttctcttgaa aagcatccat ctgggtttcc ataaaggact ggagggggct ggaaatggtt
     2581 gtataaacat ccttaccgtc catcgttcgt tgggaaactt gttctgttcc gggtacaata
     2641 ttacccagac gatccttttc ataggtaata atgccgtctg tccctgcaag aatactgttc
     2701 aaggaactct ccattccaga ggttcccagc aagctcttgc ttccatcttc attttcatgg
     2761 agctgagcta gaccgataaa actagaagca aattgtccgt ttgggtaact acgattggga
     2821 ctggttgtaa aatcaatccc cttgacctct gcagcttcca attctttttt gatagacatc
     2881 atattggcat aggtaatccc atttcccttt gctccaaagg aaacttgctt gagattaggt
     2941 tgcgagagtt gctctcttac ataggattct tccatgtcca gatacttatg aaagacctct
     3001 gcaaccttgt taaattgtgt tttttctacg taaagaatct tacccgttgc tgacttatag
     3061 ttctcatcaa tgaccgcata gacattatag gaggttgcat cctcagcaat cgggactcca
     3121 tttcggtcat aaatagtccc acgtttggca ggaactgtac gggtggtttg atgaaccttc
     3181 ttagcttcct tcgctaaatc tgttccaaag cgagtgcctg tcccaataat gaccgcaaaa
     3241 ttgactaaaa aaatggcaaa aacaaagaca gataataaac tcagactttt tccaactctg
     3301 cgtctgtttt cagccggcga tttccgattt ttggtcgcat aacggattac tctttttgtc
     3361 cacttcatat cttactccgc tattctaata ttttcattgt ttaattgcaa atcgtgtgaa
     3421 ttggcaattt ctttcaaacg ttctgcacgt aatagttcat tgacctcttg cttggcatcg
     3481 tccaattcgg tcttcttttc ctctatctgc gcattgattt ttgtcaaatc attctgcact
     3541 tgcaagagct tggtctgcat aaaaataata ctaatggcta caataagagt ggttacagca
     3601 atggaaaagt aaaaagcttt ttccacacgc gaaaaccgtt taagttgcat ctgtagtatt
     3661 tgacctgttt tttccatttt ttctgccatc tttttccctc ttacttgtga atttttctga
     3721 ccacgcgcaa cttggctgag tgcgagcggt tattggcttc taactcttcc gcacttggca
     3781 agattggctt acgggacacc aattccatct tgggcttgag atcatctggg atgaaaggca
     3841 agccttttgg aacttcaact gttgaagctt ccttgaacaa ttgcttggtc aagcggtctt
     3901 ctaaggaatg aaaggtaatc actgaaattc taccatccag agccaacata tccatagcct
     3961 gctggatgga ctcatctgcc gcccccagtt catcattgac ttcaattcga atagcctgga
     4021 aaatctgctt agcaggatga cccttcttct tgagttcctt ggcaggtttg accaacttga
     4081 taatctctgc taactcagtc gttgtctcaa tcggcttcac ttcacgcgct tgctcaatct
     4141 tacgcgcaat ctgtttagag aatttatcct caccgtattt gaagaaaata cgaaccaaat
     4201 catgatagtc atagtgatta accacttcat aggctgtcag actagcatcc tgattcatcc
     4261 gcatgtccag tggcgcatcc tttttataag aaaaaccacg ctcacgctgg tccaattgag
     4321 gactagacac tcccaagtca taacaaattc catcaatttc ctgaacacca gcttcgcgca
     4381 aacgtgcctg taaatgacgg aagttatcct tgataaaggt caccattccc ttctcaatgt
     4441 aaggtgccaa gcgtttttgc gcattgtcaa tggcattctg atcctggtca aaggcataga
     4501 gatggccttt ttcacttaat ttacttaata aatactcgct atggcctgct ccacccaaag
     4561 tcgcatcaac gtagataccg tcaggtttta cgtcaagcat atcaatcgtt tcgtggagta
     4621 agaccgttac atgatgaaat tcttttgtca tatcttatct attttaccac aaatccgacc
     4681 agcttgcact tgtcagacaa ataggacaag gagactacta tttcttcaaa aaatatgtca
     4741 aaaatacttg acactttatg tttaaaagac tataatatag tcaaatatat acgacataaa
     4801 tcagaaagga gaacagatga atcgtgtgaa agaatttcgc aaggaattgg gtatttccca
     4861 gctcgaactc gccaaggata tcggtgtctc gagacagact atcaatatga ttgaaaacga
     4921 caagtacaat ccaaccttgg aactctgtct caatctcgcc cgcagcctcc aaactgacct
     4981 caacagtctc ttttgggagg aagattttta aaaaaggagc aaactcatga aaaaagaaac
     5041 cttcactgaa aaactgatca aacgcacata cggtatttct ggtccccttg acgaatacaa
     5101 acggcgtgag gccgatagta ttgggaacca agtttttatc gtcctctttt atctgatgat
     5161 tttcggaaat cttattccac tccttctggc ctataaatac cctcaagaag tggctctaat
     5221 ctatcctcct ctgattttag tgattgccct catcgctgct ggctatgtca cctaccaaat
     5281 gaaaaaaaca gggattacag ctattgatcc agacatgctg agcgagaaag aaagtaagca
     5341 actacattac ccaggtctga aagcaggttt gttctttggt ctatggatgt tttttataac
     5401 tcctcttctt caggatactc ataggtgaag gtcaggacta ttttcattct cttctcacta
     5461 taagaaatgg tgtatcaagc attctcggtt ctattttctt cggagcgagc atacagttcc
     5521 tcatctcccg tcgcattgca aaaactaaga aaaatcaaga tgaggattag gaggtgccct
     5581 atgaaaaaag aaaagagaca gttacattat ccaggtctga aagcaggttc gatttatgga
     5641 acagtgatat tttttataat tccactcatt gataccctaa caagtgaaaa tccaaatttt
     5701 atcagttctc ttctaaatac aaaacatatt tttaaaacta tactgggagc tttctttttc
     5761 ggagtgatga tacatattgt cgactcactt cgtattgcaa gagctaaaaa agaccaggat
     5821 taggaggtgc cttatgaaat cactagctag actactgatc attcatgttt ttatcagtat
     5881 tttccttttc ttcgccctta tttcaggagc tgtttctcat acagttttac tactcctact
     5941 cctctttctt cctgcgctca ataaaggact tgagaaaata caatcaaaac ggatacctgt
     6001 cctcaacgca gccctcttct ttctcctcat atcctttcca caacttttaa ccaaccctgt
     6061 ccaatggaaa ttttcaatat tcctagtcgt aaccatcatt tcaagtttga cctacttcta
     6121 taacttttat caagtagtta aagaagtaga tcaaaaacag ttgatttagg agcttgctcc
     6181 cataatttct catttcatag gcaaataatc ccacctagct tgttctaggt gggattattt
     6241 tatttcttaa cagaaattgc aagtcatttt ccttcatttt agaaagtcga ctctttccaa
     6301 ttcacactac aatccaagac ttgttgcctt tcttcttgat tgcggccttc aatctgatca
     6361 atcaaaatct ttgtagcctg ttgtccttcc tcaaaggagg gctgaaccag cgtcgaaaca
     6421 cttggagaag aaaagcaagt ccactccgta ttgtcaaaac caatcaaccc aacttgtggc
     6481 aagttataat tcaactcttt gataacggta aagactagag gtagggccca acagttaggg
     6541 ataaatacca gagttttttc atcgggatcg atttcttttt gtaaaaattc cttaatttgt
     6601 tccaaattcg tatgcttatc ttcaatggtt agactggcgt gacgcatatt agcatctgtt
     6661 aaagcatcca caaaaccact tgcccgctca atccgagtac tcaaacgact cgtatccgct
     6721 gtaatcaaga gaaaatgttc ataacctttt tcgatacagg actgggtcat gtcataaacg
     6781 gcatcatagt tattggtttt aacccagcta gtccggtgtt catagagctg actatcaaaa
     6841 aagaccattt tcttcttttt ctcatcgatg atacgagaat attttcggaa attagaggtc
     6901 ggctgaataa taaagccgtc tactcccaag agaagcatgc tttcaatata ccggtcctca
     6961 ctctcttggc tgtaattact atttcctatc attacctggt agccattctg gctggcgata
     7021 tcctcaattc ccttaacaat ttggtttgag aaactgttgg taatatcacc gatcaaaaca
     7081 ccgattaatt ttgttcgttt ggagtttaag ctacgcgcaa caatgctcgg tttgtaattt
     7141 gtttcatgaa taactttttc aatcttttca cgtgtctctt gggacatttt ttcatatttc
     7201 ccgtttaggt aaaatgacac ggttgttttc gaggtctgag ccatttccgc aatgtctttt
     7261 atggtcagtt tcttctccaa tctaaaacac ctcctactaa tacttattat aacatattca
     7321 ctattcaaaa tgcctatttc tagcactgca agcgaatcat tctctcattt attttatcat
     7381 atactagaca ttttccacgc atttttgttc cgtcaacgag gcacaatttt tcacctacac
     7441 aaatatcctc gcttaataac agaatcagct tgtctgtttc gtcatttttt acttcaaaag
     7501 ccagagcatt ttccacttca cgatcatctg tctggtagac agaatgacgg atgatttcaa
     7561 aactctcatg cgcaatgatg gtataatcca gcatctgatt ctcaaagaat tggcgcttgg
     7621 tgagtttgct acttctttca agctcattgt atttaggaga aatgatggta tcttccaaat
     7681 caaaatcaac ttcactccat agtctcaact gattgatttt cccatcttga taggtcacat
     7741 ccttgtcaag gataaactga gtcaacgcct catgctgacc ttgacacctg atgtcatcta
     7801 ccaagagcca gacatcctct accaacatga ggatttttct cttgtgaaga taaggcaaat
     7861 caggttctgc tgaccaataa gccccttcaa tataatgcac tccctccctt tctttatggt
     7921 gacaaaacag ggagtgagga tagtattcat attcccagga tcccgtgatt ctttccggag
     7981 ctttcccatc tacaatgcag gtcgaatgac tccaagcact ctttaagaga taacgttcat
     8041 ctacctcccg ataagaataa cgcccagcat ctatgaaaat cggttggcct tgatactgta
     8101 agcaaaaact attctcgtca ctatggctat gggcacttcc cagcggaccg tttttaaaaa
     8161 atagataacg atgttcatcc ttaatgcaaa catgcccaga atcttcaaag ctcctgaact
     8221 taggctgcca agctctcttt tcaaattcct gcagtcgctt gaccttttct cgccccagga
     8281 acaggaggct aagtaaatcg actttaacat ccagaccgtt aagaaggtct tccttgttca
     8341 aaaccatagc agacaggctc aaaatctctg tcgtttctgt agaatcgcta tcaccaaaag
     8401 tcaaggtccg tccatctaga cctgtcatca tttgaatgta agtcgccatc ttttccagca
     8461 gctcttgaaa actatcttgc aagtccggaa gcaagataca caagtccagc aaggctttat
     8521 aaacctctac atgatagaga atcgactgtt caaactggct tccatcttct aaaatctgcg
     8581 tctcgatctg ctgtttcaac tcctttgaag caaaatggta agcttcttct aggtccatct
     8641 tatctgaaaa gaaatgatag gttgcaagta tcggaatcgt ttgtaaaatc ccccagttac
     8701 taagggtgta cttggcgcga taatggcttt tcataaagtc aatctgcttt tctagactga
     8761 ccaaaatttt ctctagttct tcctcctcta gcaagtcaaa tttcaagagg agcaagagta
     8821 gtttcaacca agtaaaggaa cgaatacccg tatccaaggt tctagtcatc aaagattgag
     8881 gacgaaattc tctcacctgc tcaatccagt caaatagaaa gaacttgcac ttttgaatat
     8941 agcccttatc tccttctacc agatacccta tcataaactg caagagatat tcttgtcgat
     9001 tgagcatata agaccattct ggatcatctt caaatacttg atcccatacc atcggctgga
     9061 tttgatggat ttttgaacaa ggctccatat cccaaggact atcaaacata aaacgattgt
     9121 ccatcaagcg ttcaagggaa ctcttgactt tctcatagtc ttttgaacag tgggacaaga
     9181 tataatcacg acattggttt ccatcgactc tttcaaaaaa ttgtcttctt tcttctttca
     9241 ttatctatta ccagaaaaag aactacttaa aaagcagttc ttttgtcttt cccattacac
     9301 tttccttttc tacatagatg accacacctt ttgcaatctg caaggagacc aagtcatctt
     9361 tgatagaaat gatttttcca tgaattccag acaataacaa cacttcatca ccagatgtta
     9421 aagaagctaa atactcttgt cgttgctcca tctgtttgcg aagcaacttt tgctgacgaa
     9481 tagaatgaaa gcttgacagt aaaaggggac tcactgccaa gacaatcact attccataaa
     9541 acaatgttgt atccattaag ctataatctt aagccagctt ccgataattc cgatgataac
     9601 tgttaaaata acgagtttat atgttgtcca tttcttttct ttgatcaagt agtaaactaa
     9661 aagtgtaaat agggctggta gaagagctgg agcaacctta tcaagcattc cctgaatact
     9721 tacgatactt tgtttagcgt ctgctttaac ttcccctgca gcaaaggtaa tcggcaccat
     9781 aatcttaaca gatgtcgctg ccaaaccagc aattacggtt acaccgataa tattggcaat
     9841 acgagaaatc gttgccatct gttcgcttag tttatcaatc acagttgttc ctagtttgta
     9901 tccatacaga ccagttgaca atttaatcgc tgttaaaatc gtattcatcg caaggaagaa
     9961 caagattgga ccgacaacca agccttcttg agcaaacgaa gctgcgatgg ttgagaacaa
    10021 tggagctaaa cagaattgag aaagagaatc cccaatacct gccaatggtc ccatcaaggc
    10081 catcttgatg ctacgtgttt cttttgccgg acggccattt tccaacatta caaggtgcaa
    10141 gctggtaata aaaggcagga agtgtggatt ggtattatag aattcacagt tttcttccaa
    10201 ggcttggtag aaaccttcct gatcctctcc atagtgtttt ttcaaagcag gatacattac
    10261 attggcatat cccaaccctt gatagttact atagttaaat ccattttgac aaaagaatgc
    10321 ccgcaaagac gttttaagat aatcacgttt tgttaatttg ttagatccag tcatcgtgtg
    10381 cttcctcctc taccacatga ttcgctgttt ttggcttgtt ataaaattca atcaaagcaa
    10441 agatagtacc tacaattgca ataccaattg ttgggatgtt tagataagct gcacaaacat
    10501 atcccaacaa gacaaaggga atcaactctt tcttagccat cactgacaag atcatcgcaa
    10561 aaccgatagc tgggagcatt ttaccagcaa ctgtcaaacc tgtaagcaat accggtggaa
    10621 tgtagtctac gagtttcaac aaggtatcca ttgaaagggc acccaagcaa cccaaggtaa
    10681 atccaataaa ggcaaacaac caaattgttg catttagagt gaacttaaat ttcttcaaat
    10741 tatggttttt caagtgcttc atagctgttt caggcgcacc agcacgtaca gtgtaggcga
    10801 aagtttgtaa gaattgaatc gccacagcaa tcggagtaga gagggcaaga gccgcttctg
    10861 gactgacttt accagcacta gtgatagcca tcaaggtacc aaagatacca ggtccgattg
    10921 ggtttggtgg aacagtacct ccagcaccaa caccgaatcc catataagcc aattcaccaa
    10981 tagctcccat tgcaagagca gtaggcagat cacctagaat aattccgaca ccaaatgaca
    11041 gaacaataca tctattggtg taaattccta acagcattcc actaaaacag aaagctgtcc
    11101 ataatccaat taaaatcgct tgaaatacat taatcgacat aacgatctcc tcttttaaat
    11161 atagtccata atgttgactt gaacagctcc atcatttcct gttggagttg ttttcgtatt
    11221 aaatgttact tgatgagttt ggctcaattc cttgagggcc gccttgtcct cttcacccag
    11281 gaagatggag cgtgtcactt gctctttacc aggggcattg tgaatgttcc caatattgat
    11341 ttctttgata gtgacaccac cttctaccaa ggttaaagcg tccttcacat cctttacaac
    11401 gataaagatc gtttgagcag gattagcctt gtgaatgata tcaatcacct tttgcaaagg
    11461 gaagaaacgc atggcaactg agtctggcac aactgttttc atcagagttt gttgcatctt
    11521 gtccgtgctt acttcgtcat tggcaacaat gaccgtatta caacctaggt attttaccca
    11581 aagttgtcct tgtccatgaa tcaaccgttc atcgatacgg gtcataataa tatttggcat
    11641 tgtcatattt ctcctcctac caatataggt tccagtcttt gtagaaacgg ataagggctt
    11701 ctaggtaata gtagtcaccc cagatattgc cttcatccac tcctttacct gaatgccatg
    11761 agtacacacc gtggaggaga cttgtcccac caggggtaaa ttgatcattt gcataatgtt
    11821 cgatcaagga acgaagcatg gcatgcatag catgtttata aatatctttg tcagcatcca
    11881 cctctgggag atgttttagc atttcatgaa tcccacagac ggcgatagct gttgctgaag
    11941 aatctcgtga ttgatcacta ccatcattaa aaatcaaatc ccaataggac acatgatctt
    12001 ttggcagacg attcaagaaa taattggtca cacccttaaa caagtcaaag caggactcgt
    12061 cttttaagtg acgataagtc aaaggaatac catagactcc ccatgattga ccacgtgccc
    12121 agcatgaatc atcactatac ccttgtctcg ttacaccttt aaagggttga cctgtctcag
    12181 gatcaaaata gaaggtgtgg aaggacgaag cgtcatcacg gattacatta ttagctgaag
    12241 catagaaatg gctttctgca atatcgtagt atttttgatc gcctgtttct tgataagcaa
    12301 agaataagag ttggatattg agcaagcagt cgataatcaa acggtaatgc tctttcttgc
    12361 ccaagtctcc ccaagcttga ataaaaccac ctttttcttg atagcgttca atcaacttat
    12421 ctgcagcttt caaggttgct tctctagcct ctccatctcc atttatctta tattcagcca
    12481 tacaagacgg tgtgtacaag aagccgagat catggtgatc caattctact ctcttattga
    12541 cacgatccag gaaagaaaga acatttttat gagcgatgtt tttaaatgca tcctgttgac
    12601 tgtattcata agccaaccac agttctcctg tccagaaacc attggtccat tccgtgttat
    12661 ccatgattgg atagacatta tcaaaggtag ctggcgtcgg gaaatcttcc ttgaaatagt
    12721 caaggttgag ttctaactgc cgaataacct tatcgattgc ctggccgact tcttctttcg
    12781 tcagaagtgg tacttctaag aagcgctcag gcgattttat tttttcaatc gtaacctttt
    12841 ttatcattct tttacccttt aaatacccga ttcgaaatct tcttcctctt cctctgcatc
    12901 cgttgaaaac aattctttac catttacgac tccgccctgg gccgccacta ctgatttatt
    12961 aacaacctca tcaaagctat gagccattct agcaaagact gcttccatta acatggctaa
    13021 gttcaaaccc gagagaacat tcattgtttt ggctggattt tctcccatta tggtagaaga
    13081 aaccttgaat ggcgatcctc ccaagagatc acttaggatt aaaacttctt cttcatttga
    13141 aattgcaagt aagatttttt gcttgagttc atctgctgac attccttcca caaagtcaat
    13201 cgcctccaca ttttcttgat tgcctgcaat caattgtaaa gaactataaa tccctgtagc
    13261 aaaatgtcca tgccctacaa gtacaatttt catcttttct cctttcttgt ttaggtttac
    13321 cggtttactt tatattcccc attgtaatcc tttattttta tcttgtcaat agttttttta
    13381 aaaa
//
LOCUS       DAVKZB010000045        13376 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKZB010000045 DAVKZB010000000
VERSION     DAVKZB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.045"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            319..501
                     /locus_tag="VXA80_001170"
     CDS             319..501
                     /locus_tag="VXA80_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0353673.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            446..1251
                     /locus_tag="VXA80_001171"
                     /pseudo
     CDS             446..1251
                     /locus_tag="VXA80_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1321..1683)
                     /locus_tag="VXA80_001172"
     CDS             complement(1321..1683)
                     /locus_tag="VXA80_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353674.1"
                     /translation="MPKTKTLAELADVILWGFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(2061..3941)
                     /locus_tag="VXA80_001173"
     CDS             complement(2061..3941)
                     /locus_tag="VXA80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0353675.1"
                     /translation="MVRFTGLSSKQAQAIEVLKNHISLPDVEVAVTQSDQASISIKGE
                     GGHYQLTYRKPHQLYRSLSLLVTALAEADKVEIEEQAAYEDLAYMADCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKINTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3935..4804)
                     /locus_tag="VXA80_001174"
     CDS             complement(3935..4804)
                     /locus_tag="VXA80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0353676.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEENQW"
     gene            complement(4888..7533)
                     /locus_tag="VXA80_001175"
     CDS             complement(4888..7533)
                     /locus_tag="VXA80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV0353677.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPELL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELANIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILVDDTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7624..8904)
                     /locus_tag="VXA80_001176"
     CDS             complement(7624..8904)
                     /locus_tag="VXA80_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV0353678.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            9075..11159
                     /locus_tag="VXA80_001177"
     CDS             9075..11159
                     /locus_tag="VXA80_001177"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV0353679.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKQ
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMTPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11199..12878)
                     /locus_tag="VXA80_001178"
     CDS             complement(11199..12878)
                     /locus_tag="VXA80_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0353680.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGYELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQTGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3757 a         3156 c         2697 g         3766 t
ORIGIN      
        1 atttattttc taatagatat aaaattataa ctttgctagc cgttgttata aaaagtttta
       61 ttcaatcatt atctagtaga atagagtagt acattttata aattgtcatc tctccataaa
      121 cacagtatat catttttaaa ttttaagtca aggaaaatta acccacttta atttcttttt
      181 taacccgtgt taaaaaagaa attaaagtgg gttaaaaaag aaattaactg agtaaaaact
      241 ctcgaaattc aattcgctat attatcactc aacttgaaca aataccagaa ccacaaaaaa
      301 cagatgtaaa ggaaaaatat ggacttaaaa tttgaagggg ttgatttaga atacaaaaaa
      361 gctaaaaaca atcttccaga gtctttctgg gaaacctact cagcatttgc aaatacaaat
      421 ggcgggaaaa taattctcgg gatagatgaa aaaaacattg acacctacca aagagtcaat
      481 agactccctg cgaaattatg aagcaagtaa acaactaacc gatgcacgat ttaagcgtct
      541 tgttggtgtt cagcgcacca cttttgaaga gatgttagct gtgttaaaaa cagcttatca
      601 acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc ttcttatggc
      661 cactcttcaa tatgtgcgag aataccgcac ttatgaagaa attgcggctg attttggtat
      721 tcacgaaagc aacttaatcc gtcggagcta atgggttgaa gtaactcttg ttcaaagtgg
      781 ttttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgtgacgga
      841 agtaaaaaga aatgccacgc tatgaaggct caggcgattg tcacaagtca agggagaatt
      901 gtttctttgg atatcgctgt gaactattgt catgatatga agttgttcaa aatgagtcgc
      961 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttattaagg actcatgaag
     1021 atatatcctc aagtacaaac tccacgtaaa tccagcaaac tcaagccgct aacagctgaa
     1081 gataaagcct gtaaccatgc gctatccaag gagagaagca aggttgagaa catctttgcc
     1141 aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgctttgga
     1201 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg
     1261 aagtctattg aaatactaag cctacacaga ctgattccaa ttgtttttta gatttcaatt
     1321 ttaccattct tcatagtcaa aaccatcata atctggatat tgttctggcg acgttccaac
     1381 agaacgtacg agataagctt cttcgtcctc tgtctcgatt tctctcagta cttggcattc
     1441 aaaacgccat tcatcaccga agtcgaaaat aaacttaaat ttttgtttga cacttaggct
     1501 atccagatag acattttctg tgtaacgttc ttcaacatcg tcactaacaa agctaagaaa
     1561 gtaagaatct gcatgactcc actcaacatt atccatgaaa aatgcgtgag catgatcatt
     1621 tgcaaaatca aaaccccata aaatcacatc tgctagttcc gctaacgttt ttgtttttgg
     1681 aatctatata tgtcggtagc aacctttttt aaatttgacg gaaatgatcc aagcttctac
     1741 agatgctggc ttttcagctt ctatttcttg ttttactaat tttaagtgta gcataatacc
     1801 tccaattgct catcgataga attgctctta ggaaacattc actttctgct attttatcat
     1861 aagtttgtct aactgtagca tttgaaacca gataattctt ctgtttcacg cgaaacaccc
     1921 tgcgccaagc aaggtgttct tgtttttata ctcaatgaaa atcaaagagc aaactaggaa
     1981 gctagctgca ggctgctcaa aacactgttt tgaggttgta gatggaagct gacgtggttt
     2041 gaagagattt tcgaagagta ttaagtcgta taaatcgtcg acgctgtcgc aatggtatgc
     2101 cactggttag ctgtagttgc tgcgaagtcc ttgtctgcgt agaagtcagt aaatggtagg
     2161 atttcaactt ccagctcgtc gatgcggtca agctgaccag ccagataaac ctcgatacgg
     2221 ctttctgctc gtttgatgcg ttgcaagagt ccgcccatac ggatgtcaac tgtatccaaa
     2281 ccaaagacct tgttttcttt cagccattgg tggctaaaga gggcatggaa gtcttcaatt
     2341 tggcttctaa gttctggtaa ttcttgtctg gcgatttgtt gtaaactttc tttatcatcc
     2401 gcttggtagg catgacgaat gcgtcgtccc acatctactt tgctacttaa aatagcattc
     2461 aactgggcct gagtttcaaa gagataagca tagtttccag ctttttcttt aatgttagca
     2521 agcgtctcag cagcctgagc gaagtgcggt ttgtcctgtt caggtgtcat gtgttgatca
     2581 agaatcggac aaagaatatc ctgataaaaa acatagcggt tgggattgat accgctgaga
     2641 ttgcctggta ggtctggtaa gaggttggca aggtcaatct gcataaaatc ctcaaccgtt
     2701 agaccagtat tgatcttgaa gtgcgcagac aaaccatcta ggtcattgcg atagctgagt
     2761 tctgcccaga tttgcaagct tggtaggata gagaactggg cagtttcacc accattgtct
     2821 ccccaacccg ttacgatgac ttctttaatc tgattggcac ggcaggcttt attagcctcg
     2881 atagccacta gacggctaaa atggttgtga ggtgtaaagc caatccactt ccaagctccc
     2941 cctgcaaatg caaggtcatg gctaatcttg tgatgattgc ggaaattacg gttgtatttt
     3001 tcctcgctat cctgataata atcccagtaa accagagtca cacggtcttt gagacggtct
     3061 aggtagacac gagtttcctc tggaatttcc acatcacggt cgtactggcc atccgctgac
     3121 atgagtttga agaacatatc actccacatc tggcagtgga aaccatattt gtcagcaata
     3181 tccagcacgc gctccaagtg ttggcacatg aggagactac gatccacaac accgttcaga
     3241 atcaggtagc gtcccaaacc aaccaagtgg gcttcgtcca tcccgatatt gaccttgcga
     3301 gtcttcagtt tagacaacgt ggcaaacatg ccatcaatca ggtcataaac cttttcttcg
     3361 ccaatgagaa gaatgtcctc tacatcacgg agctcctgca cttccttgac accccatttg
     3421 acaaaggccg acaagtgggc caaggtctga atacaaggaa caaaagtcat gtcaaactgc
     3481 tgggcatagg cttcgatttc ctgcaactcc tcagccgaat aagccccgcg gaaatagcca
     3541 aagtaaggct gcccttcaat ctggtaagtg tcttccatgt agagctcaaa ggttgagtag
     3601 cccatgagag ccaagacctc tatcatctgc ttggcagaag ccacattcag caccgcattt
     3661 cgagaacagt ctgccatgta ggctaaatct tcataagctg cctgttcctc aatctctact
     3721 ttatcagctt ctgcaagagc tgttaccaac agggacaagg aacgataaag ttggtgaggc
     3781 ttgcggtagg tcaattgata gtggccaccc tcacccttga tagagataga ggcttggtca
     3841 gactgagtaa cagccacttc aacatctggc agagaaatgt gattttttaa tacttcaata
     3901 gcttgcgctt gtttggaact aagtcctgta aatcttacca ttggttttcc tcctgtagcc
     3961 agttgacaag ggcaccgtag agattggcat ctgcgtgata ggtgcaggcc tggataactg
     4021 gtgcgaccgt gtattcttcg taggcatcga caaagtcttc aacagccttc ttaacacctt
     4081 ggataaaatc tggattttga ctgatagagc cacccagact gatgacatct ggatcgatga
     4141 gatactggat attgagcaag ccttgcgtca gattgcggtt catgcgctca atggcttctt
     4201 gacaaagagc attaccagct gcggcctctt ggtaaatctt gcgaccgtcc caatcagtat
     4261 gaccagattt ttcaatcacg tatcgtacca tattcccagt tgatgctagt tgcgaccagt
     4321 tattaagttt ttcagcaggg gcaagggttg tcatgtagcc aaattctcca cccagaccgt
     4381 ggcgacctcg atgaagtcta ccattgataa tcatggctcc gccaatccct gtcccaatca
     4441 cgacacaggc tgcattttca agctctggat gagctagtag ttcactgagt ccaacgcagt
     4501 tggcatcatt ttctaaatgg acaggtagct gataagagct aagcgcctca taccaagaaa
     4561 agccatggat atagggcacc gcactgaagc catcaatcac acctgtctct tgattgaccg
     4621 cacctggaac gctcatagca atcccactgt aatcctgttc tgacaagcgt tgatctagcc
     4681 acgctagtaa atcctccaag ttttcaggcg ttgaaatact tgtcttatcc agtattttcc
     4741 catcaggagt cagactggca aacttaatcc cagtccctcc gatatcaatc gttgcaatgg
     4801 tcattgtttt taccataaaa aggggcgaat cagtagttag ttcttacctt ttcgcccctc
     4861 ctttcttttg atgtttactt tttgaaatta aatttcttct tttttgatga attctgtacg
     4921 aatctcttgt ggagccaata gtcctgaatg aactggatat ggtcgctcaa gtaagtcaag
     4981 aatggtttgc tgatgttctg atatgcgcac attttcttga ctcatattat agtaacggag
     5041 gacatagcct tcttcatttt ccgccacttt aaaggctgtt ggacaaactt gcggtatgct
     5101 gagaacagaa tggctcaaga ggctaccagt cgcagccacg cttccttcct gtctagcaag
     5161 ctgaaggctg gtaaacggtg tctgcaaggc cttagctcga cgataagctg agaagcgttc
     5221 ttgggcttgg tggcactcaa gcgcaaactc gacttcaaac tcccgcaagc attgtgcttc
     5281 tggtgttggg aagtagcccc agtcacctag ctcacctgat gcacgcaaaa tagtcactgc
     5341 aatggtgtcg tctacaagga tttcgtattc attcaatccc ttgttggata cagtcacacc
     5401 tttttcatcg tcatacagac taacaaaggc ttgttggtgt tgaggatttt caggattttc
     5461 ccatgaagca gctggtttgt ttggtcgtgt caccacctca tagatacttt cagaatcatt
     5521 gcttggacgc gtgttatgag tcttgaccaa gagacggata cggtgatcct tggcagtgtt
     5581 agtaaagcga gtcttgaagc ggatttgtgg attgtcaaca aagacaatca actcagtttc
     5641 cagaggaatg tttgccaatt cttctgaccg tccagcctca cgcttcataa actcgatgat
     5701 accttgttgc tcttcttcta gcttttcatc cgcgctgaca ggcacggtca attcatgttt
     5761 gagcaagatt ttagcatagc aagctgtgtt ttccaagacc tcgtggccct taagctctgc
     5821 aaagattggc tctgttcctt ttggttggaa atagatatac tcgtttccga tgtccccacg
     5881 gtcttcaaag cggataaagt cttcataggc ttcgtgagtt gtcttgtcat agactgtgat
     5941 gttgtcatcc acactcaccg ttacgaatgg tgtatcaatc actccgtttt ggtaaatacc
     6001 ctcacggtgt tcttgttttc cttccagcaa ttggaaggtt gtccaagaaa gcggcgctag
     6061 gtgaactgga atggtcacgc gcacttgacg agcaatacga gcttggcgga acttgtcttt
     6121 tggtaaatca tactcaaaat tagctccgag gtcttcgatt gtagcctcta caggacgacc
     6181 atccaagtcc tccacacggt aacttggcaa gataagagca gccatctttt tgtagccttc
     6241 tgttgggtgc aattccttga aatcacaagt cgccacatca atcactgtgc tgacagtatc
     6301 gaccttatca tgcaagcctg tgttaatgac agtaaagaga tagtcacttt gagccttatc
     6361 cgtagcaatt ttacccttcc actcgttgag caaattactt ttaacaaagt ttcctacttg
     6421 gttgaccttg gcaaaacgcg tttccatctc gcggtgaact tcgtccacgc tacagccaca
     6481 gatactatca tgtggcgcat tctgcaaaag tgttttccaa gcataggtca actggtcctt
     6541 gtggttgtgt ccaccagtga taatagtcaa gggttctacc acttgctcta ggaggttgct
     6601 attttcttgg aaggcttgtt ttaggtaaat gcgggatgaa gaagtgttgg caagtgtgta
     6661 ccagccatct gtttcctgac tggtcaactc acctgtaaca gttgataaga gttcaggaag
     6721 cgcaccttct acagcttgaa catattcatc aaaagaacta tgaacaaaga ttacatccgg
     6781 gaagagttca tttgccacac gaatggcttc gctcagattt ttctggacag gctggtggtc
     6841 acagccgttc atcatcaacc attggttggt cgaagcgtag gcacgcacat ctgacaattt
     6901 ttgtttccag aaggtcaagg cctcatcttt gtcaactggg atttcattcc cattactgta
     6961 ccagttggca aagagaatac ctaaaacacg actaccatcc acaccctgcc agtacatttc
     7021 tgaaaactgg gatgtaaact gctcatcttc aaggacttgg ttgtcaaatc caatcggctt
     7081 cacaccacga ccaaaggccg ccacgtgaat gcctgatttt tgaagaattt gaggcgcttg
     7141 tcccatattt ccaaaggtat ctggaaagta gccaatctgg gttgatttac cccatttggc
     7201 agcttcttgt tgaccaatca aggtattgcg gacattggct tcactggaga tcaagtagtc
     7261 atcctgcaag atgtaaaagg gaccaatttt aagtttgccc tcgtcaatgt agcgttggac
     7321 cttgtcacga ttttcagggc gaatttgtaa gtagtcgtca aggacaatag tttgtccatc
     7381 caagtggaaa ctcttgaact cagggtcatt ttcaaagaga tcaaagagat tgtcaaacaa
     7441 ttccaccaac tgcatacgat ggctttcaaa aggcaagtac cactcacggt cccagtgact
     7501 atgtgagata atatgtacaa caacattttc catgaagtaa aacctcattc taaatttaaa
     7561 ttttaacgtt ttaaatgtaa acttgtgatt ctgataagaa tcggttgagc taaagcgagc
     7621 cccttagcga atatccaagt aatccaagac caactcacag aacatcatgt tagcccagga
     7681 gaaccattca cgagagtaga gggtcggatc atctacatga aagctttcgt gcatgacacc
     7741 tgtaccacca tcgcaggcaa ccagctgatc cagcaagaat tttttctctg ccttatctct
     7801 tgttgtcaat ccttggatag aaagggcgat tggccagata tagcgataga aggtatgaga
     7861 acttccgaga ccgctagcgt attctccttg gtagaaatat ggattttcag agctcaaaat
     7921 ggtacgacga gtagcttgat acacttcatc atcgaccgaa cagtagccca gatagggcgc
     7981 agctagtaga cttggtacat ttggatcatc catgatgctg gcatttccta ggccatccac
     8041 ttcaaaagcg taaatctttt cgcccttgct gttggtggtg taagcgtagt ttttgattcc
     8101 ttcttggatt tcatcctgaa gacgcttggc atcagcaata acactctggc tatcagctag
     8161 gtttaatgct gcgaagattt cttgcacata acccaagact actacagcaa acatatttga
     8221 cggaatcaag taactatact gacagcagtc atcgctcgga cgaaaggctg accaagtcat
     8281 acctgtcact gcaaagtcag gtccaaagcc atcatttacc aaggtgtctt ccttacggtc
     8341 cgtatctcgg acaaaacgat aaggagagtt cttgtggtct tgttccaccg tccacagatg
     8401 gagaatttcc ttagtcgctg cgacaaaaat ctcatcaaac tgactagtct cgccagtctc
     8461 tttccagagg agataagcca actgcaaagg atagcaaagc gaatccacct catacttgcg
     8521 ctcccagatc cagccgttaa ggtctgtgtg gtcagtctcg tggtgccctt tccagttctc
     8581 ctcaatgttg aaggagttag catagggatc cttgagtacc aaggtcatct gacgtttgac
     8641 caaacctgca atggtctgac gcaggaggga atctctttta gccacatgca ggtagggtct
     8701 gagttgggct gtcgaatccc gaagccacat agcaggaata tccccagtca agacaaaagt
     8761 tgaaccatct tctaagattt caaccgtatt gtccaaggta tcggtgtagc aacgctcgaa
     8821 aacatccacc cactctggat ggtccttagc ccgctctgct acttcatcta gccattctct
     8881 aacaatttct ttcgaataaa tcatcgtgca attccctctt tcaaacaagt ttcatgttca
     8941 gtatatagct tttgagacag aaaatacata cacaaatgat agtcattttt ctacaaaata
     9001 gttatttttg aaactccttt tctaaaggcc ctctttttct gatataatgt agaaaaacag
     9061 ctaaaggaaa gactatgaaa ccactacttg aaaccatcga tacccgcttt ggtactgcta
     9121 gcaagcatgc cttctctcgg ggaaataccc tgccatacac aggcgtgcct tttgggatga
     9181 attactttgt gccccagacc agtgaccagg atggatcttg gttttttgat ccgcatctgc
     9241 ctatctttca ggggattcga ttaactcacc agcccagtcc ttggattggg gactactctt
     9301 ggctccttct gacacctgtc accagtcaac tggggggaga cagcctcttt caccgccagt
     9361 cttcttatga tatagataaa gcttgcttcc agcctcacta tttgaagctc ttttctctgc
     9421 gttatcagat tgaaacccag ctcacaccga cttgctatgg tgcttctatt cgtttgaatc
     9481 aaaagcaagg caaagccctc tccctctatc ttcacgcagc agatgaactg acagtagagc
     9541 aagtagataa gcagactctt gccctgcgac aagaaggtaa aactgaaact aacaaaaatt
     9601 cgctaacgat gttcactgcc ctgcaaatga acacggatat tcttgctatc agccaagaag
     9661 ctggagactg gcgaattgac ttagcaagta gtcaaaccga gatgcaacta gccacttctt
     9721 tcatctctcc ttctcaagca ctgcttaatc tacctcaaga agattttgat agctgtaaat
     9781 caagtgccca agcggattgg gaaaatctcc tccatcgttt tgacattata gagataggag
     9841 aggctgaccg aaccttcttt gaccactgcc tctacagact cttcctcttc ccacagactt
     9901 tttatgagat taatgaatca gggcaagcca tccacatgga tctggctact ggtactgtca
     9961 agcctggtgt cctctttagc aacaatggtt tctgggatac cttccgcacc accttccccc
    10021 tctttgccct tatcataccg gagcactatc aacgcttttt agaaggtttc ctcaatagct
    10081 accgcgatac tggtttcctt ccaaaatggc tggctccaga tgaacggggt atgatgccag
    10141 gtacactttt agacggtatt atcgcagata gcgcctgcaa ggacatgacc cccgacctag
    10201 aaggagaact cttccaagcc atgctcaaaa cagccagcaa agccgaccct ctcggcatca
    10261 atggccgcca cggactagcc caataccaag aactaggtta cctctctacc gaccaccacg
    10321 aaagtgttag ccatactcta gactatgcct atagtgactt ttgtatcgcc agctgtgcca
    10381 aaaaactaga gaacatagaa atcgctgaaa cctacaaggc tgcttcacaa aattaccgcc
    10441 agctatttga cgctgagaca ggttacatgc gagcacgaga caatcaagga aactttcacc
    10501 ctgacttctc tccttatagt tgggggcgag actacgctga atgctctgcc attcaagcta
    10561 ctttaggcgt tctccacgac atccctggct taatccaact gatgggtgga aaagaaacct
    10621 ttagcaacta tcttttgaaa gcctgtcaag atgctcccct ctttgaaaca acaggctatg
    10681 gttacgaaat tcacgaaatg agcgagatgg ctactgctcc ttttggacaa atagccattt
    10741 ccaaccagcc tagtttccac attccttatc tattccgcta cagcgattac cctgactaca
    10801 ctgcccttct tatcaagacg ctccgtcaga aagcttttca cccaagttgg gaagcctatc
    10861 ctggagatga agacaatggt agtctctctg cttggtacat ctggtcagct ctcggatttt
    10921 atccaacctg tccaggaaaa ccaagctatg acctcggaat ccctctcttt gaccatctac
    10981 gagtctacct cgctaaagaa gataaatggc tggatatcca tactaaacaa aaccacaacc
    11041 attttaactt tgtcaaagaa tgccgactgg acaaaacact cgtatcaact attcaacacc
    11101 aagacctctt aaaagctgaa caactaactt tcaccctcag ctggttacca agtcactaat
    11161 aaccaaaaga acctttgcat cgcgcaaagg ttcttctttt atgaaacttg gacttgaagt
    11221 tgatccacaa gctgaccatc caccgttagg ttcagataga aatccaaggt tttatctcct
    11281 gcgaaataca aggttggtag cgtcagactt ttttcagtct caccagtttg aaacgcaagg
    11341 acttgaatct catcctgata ggcgacacca tggacacctg tccccggttg aatcgaaatc
    11401 ttagcttcta aagaggagct actttcacta cgttcaatcc taaaatgaag agtctctccc
    11461 tttgccacag ctaggctttt ttctgcaaat gctagttcct gaacaacttc tttttttgat
    11521 aacccaggag ttttataaag ggaaatcttg gtcaatacag ggatagattg tgcctcggta
    11581 atcatcacgc gcaccttttg tgcctctact agcggacctc gcaagaggcg cttatgacca
    11641 actgtgaagc ccctgccaaa ctcctgccag acaccatcca cctctacttg cacatggaaa
    11701 gcagaaatac gttggcctaa cttcaaatct tctctcaact caattacatc aaaagtttta
    11761 ggagaaccta agtcgagttc taactggatg ggcaagtctg catcgtttgc ccaagagctg
    11821 gtctcaaggc cgtctgtcaa atggcgacaa gcaaagtctg cggaaagagc tggaccagat
    11881 acctcagctc ccagagccaa atcttcttta tagagctcat tgcgataggt cgcaaattca
    11941 taaagtcgtt caatatcctt tgcatcaaag agcccagctt gattcggcgg aatattaagc
    12001 aagagtggag ttcctcgccc tactgagtga aagtagattt cgaccaactc ctcgagagac
    12061 ttaggatcct gatcctcatg gtagaaccag cctggacgga tggaaacatc tgcctctccg
    12121 attgaaaaaa tcgtgcccga gggatccccg tgctgaagat agttcagctc tgcttctgtt
    12181 cctagtttat caggattcac cttttgccac agtggatcac ctgcataccc tcgttcattg
    12241 ccaatccagc ggatactggt gccttctgtt gaaaaaatca agcaatcgcc ctgcaggtca
    12301 cgaatggttt caaaccattt ttcaaattca taattaacct tttgcgcgcc ctctcctctg
    12361 gcaccatcca tccaaacctc agcgaactta ccagcattcc catagttagg atttgataag
    12421 atttccttca actgagccag ataataggca ttgtagtccg cttctcggtc cacatgatag
    12481 aggggactat gggcatccca cggtgacagg tagaccccca tatccatatc aaactctgtg
    12541 gcagcttggg atacttcaag gagcaagtcg ccctttcctc tcctccaagg actgacctta
    12601 accgaataat ctgtgtgagc tgtcggataa aggacaaagc catcgtggtg cttgaccacc
    12661 aaaatcaact ttttgaagcc cgtttccttg agcacacgaa cccactcacg cgcatccaac
    12721 tgactcgggt taaagcgctc aggatcctcc tgtccagtcc cccattcttg gtcataaaag
    12781 gtattaggac cgaagtggat aaaagctgct agttcatatc ccagataagc tagctgagtc
    12841 tgacttggta acggtccatg cggtttgatt ttcttcatcg ttatatatct ccttctttag
    12901 caacttctct tgttcacaag gttatccaca ggttgtggat aataaaccat gatctaacag
    12961 ccagctcttt ccaactccaa ctggtaggct caggattttt ctcctcaata ttccctcttc
    13021 aaacagctct gtcatatgat tctgctaaca cctctcagaa tgctactctc cataattatt
    13081 ctcaggcttc tttcaggtca ttgtgtgaag accattcact ctccagactt ttccacagat
    13141 tgtgaataag tgattattct aaactatcac ctcgtcgact ataacaaaaa ataaaaagag
    13201 ggtcaataag ataattatct cttcctaaat actcagaaaa gattctataa tttgtacaat
    13261 tgaacaagaa atgcttctcc aaaatctatc ttcaaaagaa tgttgcatct ttttatcctt
    13321 tcacacaaac atttctcttt atacagatcc aagtgataac cagatgataa ttggac
//
LOCUS       DAVKZB010000046        13343 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKZB010000046 DAVKZB010000000
VERSION     DAVKZB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.046"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            107..502
                     /locus_tag="VXA80_001179"
     CDS             107..502
                     /locus_tag="VXA80_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV0353681.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            513..884
                     /locus_tag="VXA80_001180"
     CDS             513..884
                     /locus_tag="VXA80_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV0353682.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            894..3137
                     /locus_tag="VXA80_001181"
     CDS             894..3137
                     /locus_tag="VXA80_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0353683.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3344..5119
                     /gene="spxB"
                     /locus_tag="VXA80_001182"
     CDS             3344..5119
                     /gene="spxB"
                     /locus_tag="VXA80_001182"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV0353684.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5230..5577
                     /locus_tag="VXA80_001183"
     CDS             5230..5577
                     /locus_tag="VXA80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0353685.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5694..6776)
                     /locus_tag="VXA80_001184"
                     /pseudo
     CDS             complement(5694..6776)
                     /locus_tag="VXA80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6911..7192
                     /locus_tag="VXA80_001185"
     CDS             6911..7192
                     /locus_tag="VXA80_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV0353686.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            7324..8268
                     /gene="manA"
                     /locus_tag="VXA80_001186"
     CDS             7324..8268
                     /gene="manA"
                     /locus_tag="VXA80_001186"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV0353687.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(8289..9644)
                     /locus_tag="VXA80_001187"
     CDS             complement(8289..9644)
                     /locus_tag="VXA80_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV0353688.1"
                     /translation="MSEKSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLL
                     IFLISTILIGFPLLLAEFALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSF
                     YSVIGGWILVYLGIEFGKLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVS
                     RGVQKGIERASKVMMPLLFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYA
                     LGQSFFALSLGVTVMLTYASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFN
                     IQSEGGPSLLFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKR
                     AKWSVILGILTFVFGIPSALSYGVMADVHIFGKIFFDAMDFLASNLLMPFGALYLSLF
                     TGYIFKKALAMEELHLDERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGL
                     E"
     gene            9850..10598
                     /locus_tag="VXA80_001188"
                     /pseudo
     CDS             9850..10598
                     /locus_tag="VXA80_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(10728..11201)
                     /gene="tnpA"
                     /locus_tag="VXA80_001189"
     CDS             complement(10728..11201)
                     /gene="tnpA"
                     /locus_tag="VXA80_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEV0353689.1"
                     /translation="MAKTSYSLSHTKWMCKYHIVFTPKYRRKARYYKVRSDLIDIIKH
                     LCRCRGVEIIEGHMMPDHVHLLVLIPPKLSISDFMGYLKSKSALMIFDKHANLKYKYG
                     NRKFWARGYYVSTIGLNEKTVAKYIREQEKNDIALDKLSVTEYEDPFSDGGFRTR"
     gene            complement(11887..12835)
                     /locus_tag="VXA80_001190"
                     /pseudo
     CDS             complement(11887..12835)
                     /locus_tag="VXA80_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13140..>13343
                     /locus_tag="VXA80_001191"
     CDS             13140..>13343
                     /locus_tag="VXA80_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353690.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLIEAMVHKTMFDGM"
BASE COUNT         4025 a         2556 c         2873 g         3889 t
ORIGIN      
        1 aaattctatt tttcgaagaa aatattattt ctataattga caaatgtaga ttttgagagt
       61 atactgatga gtgtaattga caaatgtaga ttttggaggt atggtaatgc agatttcaga
      121 tgcagaatgg caggtcatga agattatttg gatgcagggg gagcagacca gtacagattt
      181 gattagggtt ttggcagagc ggtttgattg gtccaagtcc actgttcaga cacttttggc
      241 tcgtttggtt gagaaagagt gtctgactcg gaaaaaagaa ggcaagtcct ttgtttattc
      301 agccctttta actctagacc aaagtcggga tttacttgtc caagatatca aagacaaggt
      361 ttgttcccgt aggattagga atttgttggc tgacttgatt gtagaatgtg aatttactca
      421 gactgacttg gaagacttgg aagctgtgat ttcagagaag aaatcaagtg ctgtaacaga
      481 agtaagatgt aattgtatgt aaaggagacg tcatgttaaa tagtattgta accattattt
      541 gtattgccct tatcgcgttt atcttgtttt ggtttttcaa aaagcctgaa aaatctggac
      601 aaaaagccca gcaaaaaaac ggataccaag agattcgagt ggaagtcatg ggaggctata
      661 ctcctgagtt gattgtcctc aagaaatcag tgccagcacg cattgtcttt gaccgcaagg
      721 atccttcacc atgtctggat caaattgttt ttccagattt tggtgtacat gcgaacctgc
      781 caatggggga agagtatgta gtggaaatca cgcctgagca ggttggagag tttggctttg
      841 cttgtggtat gaacatgatg cacggcaaga tgattgtaga gtaggtggag actatgacag
      901 aaattgtgaa agcaagctta gaaaatggca ttcaaaaaat ccgtatccga gctgaaaaag
      961 gctatcatcc agcccatatc cagcttcaaa agggaattcc agctgagatt acctttcatc
     1021 gtgctactcc ttcaaactgt tataaggaaa ttctgtttga agaagaaggt atcttggaac
     1081 caatcggcgt agatgaggag aaagtcattc gttttacacc tcaagaatta gggcgacatg
     1141 aattttcttg tggcatgaag atgcaaaagg gaagctatat agtcgttgag aagactcgaa
     1201 aatctctatc tctcctgcaa cgtttttgga ttactagtat ctttactgtg cctcttgtga
     1261 ttctcatgat tgggatgttg acaggtagca ttagtcatca agtcatgcat tggggaacct
     1321 ttctagcaac aacgcctatt atgttagttg caggtaagcc atatatccag agtgcttggg
     1381 ccagttttaa aaagcacaat gccaacatgg ataccttggt tgcgctggga actctagtgg
     1441 cttatttcta tagcctagtt gctctctttg ctggtctccc tgtttacttc gaaagtgctg
     1501 gatttatcct ctttttcgtt cttttgggag cagtttttga ggaaaaaatg agaaaaaata
     1561 cgtcccaagc tgtggagaaa ttactggact tgcaagctaa aaccgcagaa gtcttgagtg
     1621 atgatagtta tgtccaagtt cctttggaac aagtcaaggt aggcgacctg attcgagtgc
     1681 gtcccggtga aaagattgct gttgatggtg tcgtagtaga aggtgtctct agtattgacg
     1741 aatccatggt gacaggtgag agtctgcctg tggacaagac agttggagat actgtcattg
     1801 gctcaaccat caatcatagt ggaacgcttg tctttagagc agaaaaagtt ggctcagaga
     1861 ctgttttggc tcagattgtg gattttgtga agaaagctca gacaagtcgt gcgccgattc
     1921 aggacttgac ggataagatt tcagggattt ttgtcccagt agttgtcatt ttaggaatca
     1981 tgaccttttg ggtttggttc gtcttgctca gggatagtgt ggtcgtgctt ggagctagct
     2041 ttgtgtcctc tcttctctac ggagtggcgg ttttgattat cgcctgtcct tgtgccttgg
     2101 gacttgcaac accgacagcc cttatggtgg ggacaggacg tagtgccaag atgggggttc
     2161 tcctcaaaaa tggaactgtc ttacaggaaa tccagaaagt tcaaactctt gtctttgata
     2221 agaccgggac tttgacggaa gggaaacctg tggtaacaga tatcatcggc gacgaagtag
     2281 aagtgtttgg attggcagcc tccttggaag atgcttctca acacccactg gctgaggcta
     2341 tcgttaagcg agcgagtgaa gctggacttg agtttcaaac tgttgaaaat tttcagtcct
     2401 tgcacgggaa aggtgtttca gggcgaatca atggaaaaca agttttactt ggaaatgcta
     2461 aaatgctgga tggtatggat atttctaata cttatcaaga taaactagaa gaactagaaa
     2521 aagaagctaa gacagttgtg ttcttagctg ttgacaatga aatcaaaggc ttgcttgctt
     2581 tgcaagatat tcctaaggaa aatgctaagc tagccatcag tcagctaaaa aaacgtggtc
     2641 tccgaacagt catgctgaca ggagacaatg ctggtgtggc gcgtgctatt gcagatcaaa
     2701 tcggaattga agaggtcatt gcaggcgtct tgccagaaga aaaagcccat gaaatccata
     2761 aactgcaaca gtcagggaaa gtagcctttg ttggggacgg tatcaatgac gctcctgccc
     2821 ttagtgtagc agatgtgggg attgctatgg gagctggaac agatatcgcc atcgagtcag
     2881 cagatttggt gttgacaacc aataatcttt taggagtggt tcgtgccttt gatatgagta
     2941 agaaaacctt tcatcgaatt ctactcaatc ttttctgggc ttttatctac aatgttgtcg
     3001 gaattccgat tgcagcagga gtcttttcag gtgttggact ggctctcaac ccagaattgg
     3061 caggtctagc catggccttt agttctgtat ctgttctgac cagttccctt ctgttaaatt
     3121 ttagtaaaat agactaaaag taatgactag atttctttgt tataaaacag aaataacaga
     3181 actttcaaaa ctgtattaga atagagaata gagagttttg agcagatttt tagaaaagtc
     3241 agcataaata tgatacagtg gaatagtaaa aatttggaga acgtttccaa ttctatgtaa
     3301 tcgtattctc caaatctaaa aaaattgaag gagagttatc attatgactc aagggaaaat
     3361 tactgcatct gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata caatctacgg
     3421 tatcccatca ggaacactca gctcattgat ggacgctttg gctgaagaca aagatatccg
     3481 cttcttacaa gttcgccacg aagagacagg tgctcttgca gcggttatgc aagctaaatt
     3541 cggcggctca atcggggttg cagttggttc aggtggtcca ggtgcgactc acttgattaa
     3601 cggtgtttac gatgcagcta tggataacac tccattccta gcgatccttg gatcacgtcc
     3661 agttaacgaa ttgaacatgg atgctttcca agagcttaac caaaacccaa tgtacaacgg
     3721 tatcgctgtt tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag taattgacga
     3781 agcctgccgt gctgcaattt ctaaaaaagg tccagctgtt gttgaaattc cagtaaactt
     3841 cggtttccaa gaaatcgacg aaaactcata ctacggttca ggttcatacg aacgctcatt
     3901 catcgctcct gctttgaacg aagttgaaat cgacaaagct gttgaaatct tgaacaatgc
     3961 tgaacgccca gttatctatg ctggatttgg tggtgttaaa gctggtgaag tgattactga
     4021 attgtcacgt aaaatcaaag caccaatcat cacaactggt aaaaactttg aagctttcga
     4081 atggaactat gaaggtttga caggttctgc ttaccgtgtt ggttggaaac cagccaacga
     4141 agtggtcttt gaagcagaca cagttctttt ccttggttca aacttcccat ttgctgaagt
     4201 ttacgaagca ttcaagaaca ctgaaaaatt catccaagtc gatatcgacc cttacaaact
     4261 tggtaaacgt catgcccttg acgcttcaat ccttggtgat gctggtcaag cagctaaagc
     4321 tatccttgac aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa acgttaagaa
     4381 caaccaaaac tggcgtgatt acatgaacaa actcgaaggt aaaactgagg gtgaattgca
     4441 attgtatcaa gtttacaatg caatcaacaa acatgctgat caagacgcta tctactcaat
     4501 cgacgtaggt aacactactc aaacatctac tcgtcacctt cacatgacac ctaagaatat
     4561 gtggcgtaca tctccgctct ttgcgacaat gggtattgcc cttcctggtg gtatcgctgc
     4621 taagaaagac aatccagatc gccaagtatg gaacatcatg ggtgatggag cattcaacat
     4681 gtgctaccca gacgttatca caaacgttca atacgacctt ccagttatca accttgtctt
     4741 ctcaaatgct gagtacggct tcatcaagaa caaatacgaa gatacaaaca aacacttgtt
     4801 tggtgtagac ttcacaaacg ctgactacgc taaaattgcg gaagctcaag gagctgttgg
     4861 attcacagtt gaccgtatcg aagacatcga tgcagttgtt gcagaagctg ttaaattgaa
     4921 caaagaaggt aaaactgttg tcatcgatgc tcgcatcact caacaccgtc cacttccagt
     4981 agaagtactt gaattggatc caaaacttca ctcagaagaa gctatcaaag ccttcaagga
     5041 aaaatacgaa gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag aaggattgca
     5101 atcacgcgca attaaataat tcctctcgcc gaaaatcaaa tatgaaactt gtagacttta
     5161 ttctttgatt ttcatagagc agaactttaa aagatcggag caatccggtc tttttatgga
     5221 ggagagtaaa tgaatttaaa tcaattagat attatcgttt caaatgttcc ccaagtctgt
     5281 gctgacttgg agcatatttt ggataaaaag gcagattatg ctgatgatgg ttttgctcag
     5341 tttacgattg gtagtcactg ccttatgttg tcacaaaatc atttggttcc tttggaaaac
     5401 tttcagtcag gaatcattat tcatatcgag gttgaggatg tagaccagaa ctacaaacgg
     5461 ttgaacgagc ttggtataaa ggttttacac ggaccaactg taaccgattg gggaacagag
     5521 tccttattag ttcaaggacc tgctggtcta gtgcttgatt tttatcgtat gaaataggtc
     5581 gcgtagtcat ttgacgttaa ccttagctat ttttaaaata gaaattgaga atatgaacta
     5641 ttgaaagatc ggagtaatcc ggtcttttta tataaagaaa ttgcatataa tactcatctg
     5701 ctttcaaaaa gcattctagt ccatctccga ttaacgatgg actttatcac ctccttctcc
     5761 agtccttgta tgacatcttg gagttgattc atgacatctt ccaaagttcg aaaggcttta
     5821 ttcttaaatc cacgtttacg aatctctttc cacacttgtt caatggggtt catctctggt
     5881 gtgtatggag gaatgaaggt aaaaccaata ttagtcggaa tctttaagat acttgattta
     5941 tgccatatag catggtccat aacgagtaaa agataatcat ctggataagc ttgtgaaagc
     6001 tcttctaaaa aggcgttcat ccactcagta ttacatccac cagctattaa gaaaaatgat
     6061 tcgcctgtat gggcagcaac agctccataa caatagcgaa attctcgtat atagtgacta
     6121 tggacatgtg gacctactcc tattggagac caacaagatc ccagtttact gattctaccg
     6181 aaaccagctt catcttggta catcaagcga accttatgaa aacgtctact atatttaaaa
     6241 cgctttcttg tcttcttgaa ttgagatttt atttttagac gcgacaatgg tttgagcgtc
     6301 tgctttccta ggatgttctg gacttggcgt aatatttcgc caaccatggc acttcaacag
     6361 ttgatagaag gtatcacgtg tgtaggaatg acctaactcc tttttataag cctgaaataa
     6421 ggcatcaatt gtaacaaatt ctcctgcctc tgtagccttc aaatgacggg caagaaagac
     6481 tttctattgc tcaaccgtca tatatgcatg gttacgacca ccacgtgttt cttgaaggag
     6541 agagtcgagt ccgaactcct catatttttt tacatttcgc caaatcgttg tttgattaca
     6601 gtctaaaagc tctataatct ctttataaga tttgcccatc agacgaaata taatagattg
     6661 aaactagaat agtacacctc tacttctaaa acattgttaa aaatcgattt gactgtcctg
     6721 atcgatttgt cctgttcttg tttcgtttta ctatagaacg atttgaaggt gtttatgata
     6781 tttagctgta ctagagtctt ttaaaagtgt tttgatggtt tggatttctt ctttaattga
     6841 tttcatatta ctattgtata atgctttttg attttagtat agtattatcg tagaaaatgg
     6901 actcggtgct atggatacac cggatgagaa tggttatgta gcagatgact atcggattac
     6961 ttacttagag gcccacatca aggccatgcg agatgccatt tacaaagacg gggttgactt
     7021 gcttggttat acgacatggg gctgtatcga ttcggtttca gctggcacag gggaaatgaa
     7081 taagcgctat ggctttatct atgtagatcg agataatgta ggaaatggga ctctcaaacg
     7141 tagcaagaag aagtccttct attggtacat gtcatttata gcaatggtgt aagcatcgag
     7201 tagagcacat ttgtttacta tttttataaa atcttctccc tgctttataa gatgtaaaaa
     7261 agagttcaat tcgaactctt tttttgctat aatgagggga gaaaaatcag acaggagatc
     7321 gacatgtcag aaccattatt tttacaatca gttatgcaag aaaaaatctg gggtggagcc
     7381 aagctacgtg atgagtttgg ctacgacatc ccaagtgaaa aaatcggaga atattgggcc
     7441 atctcagccc atccaaatgg agtctctaaa gttgccaatg gtcgttacga gggaacagat
     7501 cttgctactt tgtatgcgga acaccgtgaa ttatttggca atcgtccaga acctgtattt
     7561 ccacttttga ccaagatcct cgatgccaac gactggctca gtgtccaagt tcacccagac
     7621 gatgcttatg gactcgagca tgaaggcgaa ctcggaaaaa cagaatgctg gtacattatc
     7681 gcagcggatg aaggttcaga gattatctac ggtcacaatg ccaagtcaaa agaagaactc
     7741 cgccagcaaa tcgaggacaa gaactgggat gacttgttga ccaaagtccc tgttaaggct
     7801 ggagatttct tctatgtacc aagcgggact atgcacgcta tcggggcggg tatcttaatc
     7861 cttgaaaccc agcagtctag cgataccacc tatcgcgtct atgactttga ccgcaaggat
     7921 gacaaaggca acttgcgtga acttcatctt gaaaaatcca tcgatgtctt gaacattggt
     7981 gagccagcaa atagccgtcc tgtaactgtt aaagcagatg atttgcgttc cactctcctt
     8041 gtatctaatg atttcttcgc agtttacaag tgggaaatta ctggaaaagt tgactttgaa
     8101 aagacagctg actacagctt attgagtgtc ttagctggtc aaggtcagct aactgttgac
     8161 gggaaaaatt atccaattca aaaagggaga cactttatcc taccaagtga tgttgaagct
     8221 tggactctgg aagggcaagg tttggaattg attgttagcc atccataaaa agaaagggcc
     8281 tgagttcact actcaagtcc tttttgatta cataaattgg gcaatgaaga ccacaatgat
     8341 gatgattgga atgacgaaac gaagaaacga agaaggaaga gccagacttg gaacagtcct
     8401 tgtttccatg ctctttcatc gagatggagt tcctccattg caagagcctt tttaaagata
     8461 tagcctgtaa aaagtgaaag gtagagagct ccaaatggca tgaggagatt ggaagccaag
     8521 aagtccatag cgtcaaagaa gatcttacca aaaatgtgaa catccgccat gacaccgtaa
     8581 gatagggctg aaggaatgcc aaagacaaag gtcaaaattc ctaaaataac actccatttg
     8641 gcacgtttgc tgttatcctg gttggtgata ttgcccacat tgatctccag catcacgaca
     8701 gaagaagtga cagtcgcaaa gaggaagagg acgtagaaaa tggttccaaa aggcatcttg
     8761 tcaaagagtt gaggtaagac gataaagagc aagctcggtc ccccttcaga ctggatattg
     8821 aagggggatc gagcttggaa aatggctaga cctgccatga tggatatcga gatattcatg
     8881 gctacgatgg agattcctga ctggactaga ttggttttct tgtccaagta agaagcatag
     8941 gtcaacatga ctgtaacccc tagtgagagg gcaaagaaag attgtcccag agcatagagg
     9001 agaccagcgc tagtcagttt tgaaaagtct ggtttgagga agtaaagaac cccttccatg
     9061 gcatttggca aactgagaga gcgaccgatg ataacaacaa agatgataaa gagcaggggc
     9121 atcatgactt tcgaagctct ttcaatccct ttttgaaccc cacgtgatac aataaagata
     9181 tttaataaga taaaggctgc ttgagctact agggcaatgg ctggatttga aatgattgaa
     9241 gtaaataact gagcataatc acccgttcca ccaagttgga acaatttccc aaactcaata
     9301 cctagataga ctagaatcca tcctccgata acactgtaaa aagataagag gataaagagg
     9361 gcaaaggcgc caatccaacc gataaagttg tacttgttat tcttgcccag ttttccaaag
     9421 gttttgatag cggaaacgcc agcactacgg ccaagggcaa actcagccag caggagaggg
     9481 aaaccgatta aaatagtgga aatgagaaag attagtaaaa agcctccacc gccattagca
     9541 gcagtcatgt agggaaactt ccaaacggaa ccaagcccga tgaccgagcc agcagatgct
     9601 agaataaagc caagtttcga cccccattgt gatttttctg acatagttaa actctaatct
     9661 cctttttcaa aataagaaaa agctacttga gttgtcaaat agccttctgt ttgattgata
     9721 gacttgacta tcctattatg ttttctaagg tctgtcaaga aaaccatttt caagttttca
     9781 cacctttctc aaaaacgctt gactctgacc taaggggagg ggttatacta tcattgtaag
     9841 gaggaaatca tgtaccatat aaaagaagct gcgcagcttt caggtgtctc tgtcaagacc
     9901 ctgccctgca tcactatgac aagataggac tcttggtccc cttaaagtcg gaaaacggct
     9961 atcgaaccta tagtcaagag gatttggaac gccttcaggt cattctttac tacaaatatc
    10021 taggcttttc tttagagaaa atagcagaac tgttaaagga agaaaggaca gatttattgc
    10081 tccatttgac taggcagttg gactatctaa ctcgcgaaag gcaacatctg gataccttga
    10141 tttccacctt gcaaaaaact attcaagaac aaaaaggaga aagaaaaatg accattgagg
    10201 aaaaattcac gggatttagc tatcaagaca atcaaaaata ccaccaagaa gcggtagaga
    10261 aatatggtca agaagtcatg ggacaagcgc tcgaacgcca aaaaggtcac gaagacgagg
    10321 ctacggccgc ctttaaccaa gtctttcaaa ctttggcaca aaatcttcaa gttggtttac
    10381 ctgcaacagc aaccgaaaac caagagcaag cagccaagct cttgcaagct attcgcactt
    10441 atggatttga ttgctctatt gaggtattcg gtcatatcgg taaaggttac gtctacaacc
    10501 cagagtttaa ggaaaacatt gacaagtttg gttctggaac agcccagtac acgtcagatg
    10561 ccattgcggc ttacgttcag agaaatgcag agtcataacc acccgtgaaa acgggtgtct
    10621 tgtacaccgg ctataagccg tttctctcca gtggcgtcta aagacgctcg ctgaacttcg
    10681 ttcaggtcgt gctatattac ttgactaatg cccgtcacaa cgggctttta ccttgttcta
    10741 aatccgccat cagaaaatgg atcttcatat tctgttacac tcagcttatc gagtgcaatg
    10801 tcattctttt cctgttcgcg aatatattta gctacggtct tttcgtttag tccaatggtg
    10861 cttacatagt agcccctagc ccagaacttt cgatttccat acttatattt taaattagcg
    10921 tgcttatcaa atatcatcaa tgcactttta ctcttgagat accccataaa atcggaaatc
    10981 gaaagtttgg gaggtatcag tacaagcagg tgtacatggt ctggcatcat atgaccttcg
    11041 ataatctcca cgccgcgaca acggcagaga tgtttgataa tatctatcaa atccgacctg
    11101 actttgtaat atctcgcctt acggcgatac ttaggtgtaa aaactatatg atatttgcac
    11161 atccatttgg tatgtgataa actgtaactg gttttagcca tttatttttc ctcctttatc
    11221 taacctgaac aattagatta tacttggaaa aataaatgga agcttgaagc cttgtcagcc
    11281 accagcatag ctggtggttt tcttgttttt ctatacgaga aaaactggct tgaagccata
    11341 ataaatgagc ctgggacaaa atagttctcg accacaaaaa agctagagat ttccaattgt
    11401 ggaactctag ctttttaatt ttgagtcgtt ctcttattgt attttaggag cttattggcc
    11461 atgagtacca atcccatatc aattttcact tgacgcttac ctctcagatt acatttcttg
    11521 taccccaaac aagccgtcag gagataaaag ttcctgacat tctttagctt tcaagttttg
    11581 acagccttcg ttcatataca gtcccttttg aggggctgct tcagattgaa cagcgtagta
    11641 aaccttgatt tcctgttgaa agttcgtctg tgttttctga tgtttgatat ggtgaaaacg
    11701 atagcaccag ccgtcaggat gtgtatagct atccttcttg tcatcatagt gccaattcgc
    11761 tcagtttcta gctgactgtt tatatagtgg attgaaataa gatgtgaaca aatatattaa
    11821 gaaagtcaat ttaatttcta gcaatgtttt agaagtttta gtgtactgtt tcaacttcaa
    11881 tctattatac cctctcttct gttctttatc aaacatggca tatttaatca gatggttcac
    11941 ctccttttcc tctaaacgaa ggaggttctc ttcacttcca tatccagcat ctgcgacaac
    12001 tgtcttcaat tcatgcgcat aaaagtggca tctggatcgg ttttggaaaa gctgttaggt
    12061 ccttgaaatg tctcctgata gttctcatat tttttagcac atactgaaaa atcctccttg
    12121 actttacgca agactttctt gagtttacga cgttgggttt tacgttcatc cttcccttta
    12181 acgggtatct cctcaatgtc ttggttcaat ttttccaatt cttcttcaag gatttgagcg
    12241 aatgcaagca actggaggag ataagctctt gtgtatccag ttcgatagct tgatgaatca
    12301 ggggagtgat ttcttcttga aaatagacct gtatctgttc ttgaagtttg gcggagaatt
    12361 tctctgtcgc gttcttccac acgaaactat acttgttggc attggcttca atcttagtcc
    12421 cgtcaataaa cagacaatct aaggtcacta actcttccat ttttaaacga agattgaggt
    12481 cgatgaaaag atcacgaatg agttcttcca tcccttcagc gacacgaaaa cgattgatag
    12541 tgcggtagct aacaaccaac tgtcctgtta ggtactgcat agcgagattt tcaatcatcc
    12601 ttttttcgat ttttcgacca gagaaaatcc cttgtgaata ggcaaataga agggtagata
    12661 caagcatttt agggtgataa gacgggtgac caaaggcatg atagaaggcg tggaagtgac
    12721 aatcctccaa agtattcacc actttttcaa tagtaaagac gagatggtct tgtggcaaga
    12781 aagaactgat ttctagtggt aaagttgttt gatttgtgtt atagtgaata tgcatgggat
    12841 agcctttcta aatggtttat tgtgtaattc tatagcgaat tgaatcaaga acagtacact
    12901 acgactgcta aaaaaattct aggaattaaa ttgactttcc taaccgattt gttcacatct
    12961 tatttcaatc tactttattt taccaagact atcgcaacca tgcaaaaaag caacggcaaa
    13021 tccgttgctt tttttggaaa taagagacta ttgtcccaga ctcattattt ttttgaaagt
    13081 taatattgag ttaacataga aatgttatag tgacaggaga caaaagaaag gatactaata
    13141 tggtattagc gattatttta gtaacattcc ttattcgatt gattttttta aagcgttcga
    13201 tagagaatga gaaacgaatc cttagcaatg gcgggaaaga atttggagtt gagaatacaa
    13261 aacgattaac gttggctcat attgtttttt atctctcttg cctcattgaa gcaatggtgc
    13321 acaagacaat gtttgatggt atg
//
LOCUS       DAVKZB010000047        13170 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKZB010000047 DAVKZB010000000
VERSION     DAVKZB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.047"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            59..1318
                     /locus_tag="VXA80_001192"
                     /pseudo
     CDS             59..1318
                     /locus_tag="VXA80_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1414..2688
                     /locus_tag="VXA80_001193"
     CDS             1414..2688
                     /locus_tag="VXA80_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV0353691.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRQEKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYIVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2698..3576
                     /gene="truB"
                     /locus_tag="VXA80_001194"
     CDS             2698..3576
                     /gene="truB"
                     /locus_tag="VXA80_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV0353692.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3817..4119
                     /locus_tag="VXA80_001195"
     CDS             3817..4119
                     /locus_tag="VXA80_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0353693.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4227..4865
                     /gene="udk"
                     /locus_tag="VXA80_001196"
     CDS             4227..4865
                     /gene="udk"
                     /locus_tag="VXA80_001196"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV0353694.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4993..6333
                     /locus_tag="VXA80_001197"
     CDS             4993..6333
                     /locus_tag="VXA80_001197"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV0353695.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQVTIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQWDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     RQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAETKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKNVKTKEI"
     gene            6311..6523
                     /locus_tag="VXA80_001198"
     CDS             6311..6523
                     /locus_tag="VXA80_001198"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV0353696.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6520..7395
                     /locus_tag="VXA80_001199"
     CDS             6520..7395
                     /locus_tag="VXA80_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV0353697.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7388..8203
                     /locus_tag="VXA80_001200"
     CDS             7388..8203
                     /locus_tag="VXA80_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV0353698.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVFNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8196..8627
                     /locus_tag="VXA80_001201"
     CDS             8196..8627
                     /locus_tag="VXA80_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0353699.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8634..10301
                     /gene="recN"
                     /locus_tag="VXA80_001202"
     CDS             8634..10301
                     /gene="recN"
                     /locus_tag="VXA80_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV0353700.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10303..11031
                     /locus_tag="VXA80_001203"
     CDS             10303..11031
                     /locus_tag="VXA80_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV0353701.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGVVAED"
     gene            11110..12933
                     /gene="lepA"
                     /locus_tag="VXA80_001204"
     CDS             11110..12933
                     /gene="lepA"
                     /locus_tag="VXA80_001204"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV0353702.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13014..>13170)
                     /locus_tag="VXA80_001205"
     CDS             complement(13014..>13170)
                     /locus_tag="VXA80_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353703.1"
                     /translation="SKHSFEVVDETDEVSSKHDFEVVDETDEVSNHTYGKATLTWFEE
                     IFEEYKN"
BASE COUNT         3909 a         2425 c         3159 g         3677 t
ORIGIN      
        1 gtaaactata actcaaggtg ctacaatatc cttaataaaa taatatggag gtcaccttat
       61 gactcgtgat tttaaatttg aaactctaca actacatgct gttcaagttg tggctccagc
      121 tactaagtct cgtgcagtgc cgatttatca aacaacattt tttgtttttg atgacacgta
      181 ggaaggtgcc gatatgtttg ccttgaggaa accagggaac atttatactc gtatcaccaa
      241 tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat caggtatgac
      301 tgcagtgact tatactattt tggcgattgc ccatgctggt gaccatgtag tggctgcttc
      361 gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt atggtatcac
      421 aacaaccttt gtcgatattg ataatttgga ggaagtagaa gcagctatca aagacaatac
      481 caagcttgtc ttgattgaaa ccttgggtaa ccccttgatt aatattccag acctggaaaa
      541 actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata cttttgcaac
      601 accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact ctgcgactaa
      661 gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg gtcgttttga
      721 ctggacggct tcagggaaat tccctcaatt tgttgacgag ggtccaagct gccacaattt
      781 gagttatact cgtgatgtgg gtgcagcagc ctttattata gctattcgag ttcaattgct
      841 tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac aaagacttga
      901 aaccctttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg ttgattttct
      961 tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata gtccttatca
     1021 tgccttggct gagaaatact taccaaaagg tgtgggctca atctttacct tccatgtcaa
     1081 aggtggggag gccgaagccc gcaaggtcat tgataatttg gaaatcttct cagaccttgc
     1141 taacgtggca gatgccaagt ctcttgttgt tcatccagca acaaccactc acggtcaatt
     1201 gtcagacaaa gacctagaag ccgcaggtgt tacaccaaac caaatccgct tgtcgatcgg
     1261 acttgaaaat gtagaggatt tgattgaaga tttgcgcttg gccttggaaa aaatttaaag
     1321 taaaagaaga taaacagtgg gttttgactc actgtttttg attttccctc aggcatgata
     1381 taatggttaa agaagtctag aaagaggaac gatatgaacg aaatcaaatg tcccaactgt
     1441 ggggaagtct ttacagtaaa tgaaagccag tatgctgaac ttttatccca agtgagaacg
     1501 gcagagtttg ataaggaact acatgatcgg atgaggcagg aactggcctt ggctgagcaa
     1561 aaggctatga atgagcaaca gactaaactg gctcagaagg atcaagaaat tgcgcaattg
     1621 cagagtcaga tccaaaactt tgatacagaa aaagaattgg ccaagaaaga agttgaacag
     1681 acaagccatc aggctctctt ggctaaggac aaggaagtac aggctttgga gaaccaattg
     1741 gccactttgc gtttggagca tgaaaatcaa ctgcaaaaga ccctttctga cctagaaaaa
     1801 gaacatgatc aggtcaaaaa tcagctcctc ttgcaagaaa aggaaaatga gttgtctttg
     1861 gcttctgtta agcaaaacta tgaagcccag cttaaagcag ctagtgaaca agtcgagttt
     1921 tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct agaacagtat
     1981 gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta ctttgagaag
     2041 gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga gtgtgatgaa
     2101 aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga cggaacagag
     2161 aagaagcaca aaaatgcaga tttttacaag gaattggaca aggaccgtca ggagaagaac
     2221 tgtgagtatg ccgttttggt gaccatgctt gaggccgata atgactactt taacacaggg
     2281 attgttgacg tcagccacga gtatgaaaaa atgtatattg ttcgtcctca attctttatc
     2341 caattgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa gcaggagttg
     2401 gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagactt ggatgccttt
     2461 aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa agctattgat
     2521 gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac cacatctgaa
     2581 aaccaactcc gtttagctaa caacaaattg gaagatgtct ctgttaaaaa attgacccgg
     2641 aaaaatccaa caatgaaagc gaagttcgaa gcactgaagg gggagtagaa agcaaaaatg
     2701 aacggtatta tcaacttaaa aaaagaagca ggaatgacct cgcatgatgc ggtttttaaa
     2761 ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg gaaccttgga tccggatgtg
     2821 gtgggtgttt tgccgattgc ggttggcaag gcgacacgca tggtcgagtt tatgcaggac
     2881 gagggtaaga tctatgaggg ggaaattact ctgggttatt ccacgacgac tgaggatgct
     2941 agtggggaag tgatcgcaga aacccctgtt ttgtctccct tggatgaaaa gcttgttgat
     3001 gaagcgattg ctagcttgac tgggcctatt acccagattc cccctatgta ttcggcagtt
     3061 aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg gtcaggaagt ggagcgtcca
     3121 gaacgtcagg tgaccattta tcaatttgag cgaacaagtc cgatttctta tgatggccaa
     3181 cttgcccgat tcacttttcg tgtaaaatgc agtaaaggga cttacatccg tactttgtca
     3241 gttgatttgg gtgaaaagct tggttatgcg gctcatatgt cccatttgac tcgtactagt
     3301 gctgctggct tacaattaga agacgctctt accttggagg aaattgctga aaaagtagag
     3361 gctgggcaat tagattttct ccatccttta gagattggga caggtgacct tgtcaaagtt
     3421 ttcctaactc cagaagaggc tacagaagtt cgctttggtc gttttattga gctagaccaa
     3481 acggacaaag aactggctgc ctttgaagat gataaattgt tagccattct agaaaaacgg
     3541 ggcaatctct ataagccaag gaaggttttt agctagatcg tttaggaata aaaatcgggt
     3601 gatagataac aattgcttga taaaacccca tactaatagt agaatggttt tgggaattat
     3661 aatattccaa ttgttgcgag ttgtagatac tcaaataatc tatatagaaa tttagaggtg
     3721 tgaaatgaag caacttaaaa ttctttcaga taaatattta gagtccatta caggttctgg
     3781 tgggaactta ggcccaggat ttggtgtgat aattccatga tgcgaaatga gtttcgagaa
     3841 agggtggagc aacttcttca acaaaaagaa ataaatgaaa atagtgagtt gagtcacctg
     3901 tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat accaatcggt catggccaat
     3961 ttgagtcaag ggttgtcact ttacctcatg acgcatcatt accaggcacc taagtctgtc
     4021 attgattttg gtttatggat tgccaaagct cctagccagg aaagagggag actggctttc
     4081 ttgcagatgc ttgctcaaac cctacagggc tttcgttaaa actgtcgaac atcagaggga
     4141 agactctggt gttttttgct gatgagacta taaattttgt aaaaaatgtg atagaataga
     4201 cgacggataa aataacggag gatagcatgc aaaatagacc aatcattatt ggagtgacag
     4261 gtggttctgg tggtggtaaa accagtgttt caagagccat tttatcgcat tttcctgatg
     4321 aaaagatttc catgattgag catgattcat actacaagga tcagtctcat ttgacctttg
     4381 aagagcgtgt caaaaccaac tatgaccatc cttttgcctt tgatacagac ttgatgatcg
     4441 agcagattaa ggaattgttg gcagggcgtc cggtggacat cccgacctac gactatacag
     4501 agcatacacg gagtagcaag acctatcgtc aggagcctca agatgtcttt atcgttgagg
     4561 gcattttggt cttggaggac aagcgtctgc gcgatttgat ggatatcaag atttttgtgg
     4621 atacggatga cgatgtgcgt attattcgtc gtatcaagcg tgatatggag gagcgtggtc
     4681 gtagccttga tagcgttatt aaccagtact taggtgtggt caaaccaatg taccaccagt
     4741 ttatcgagtc aactaagcgt tatgctgata tcgtcattcc tgaaggggtt agcaataccg
     4801 tggctatcga cctgttgacg accaagattg caaagatttt ggaagaagct cgaaacagca
     4861 aataatcaca tgtggaggcc ttacctcctt tttctatttt tccctcataa tggtacataa
     4921 atggagattt ttaggcatat ttttggtata ataataccca tgaaagagca agtaaatgaa
     4981 tagtaggtgg agatggaaaa gtatttatcg gtaacaactt tgaccaagta tctgaaaatg
     5041 aaattcgata aagacccata cttggaacgg gtctatttaa ctggtcaagt ttccaacttt
     5101 cgtaaacgac ctactcacca atatttctcc ctaaaggatg accatgcagt tattcaagtg
     5161 accatctggt ctgggattta tcagaaatta gggtttgacc tggaagaagg aatgaagatc
     5221 aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta gctactccat catcattgaa
     5281 aaagctgagc ctgatggggt tggggcgctt gcgattcagt ttgaacaact taagaaaaaa
     5341 ttgacagaag aaggcctgtt tcaagaacgc ttcaagcaag ctctacccca attttctaag
     5401 agaattgggg tagtaaccag ccgtagtgga gccgttattc gagatattat cacgaccgtc
     5461 agcaggcgat tcccaggtgt tgacatcctt ctttatccga ccaaggttca aggtgaaggg
     5521 gctgcggagg aaattgctag aaatattgcg cgtgctaatc aatgggacga tttggatttg
     5581 ctcattattg gtcgtggagg tggttctatt gaggatctct gggcctttaa cgaagaaatt
     5641 gtggtacgag ctatttttga atctcgtttg ccagttatct ctagtgtggg gcatgagacg
     5701 gatgtgacct tggcagattt tgtggcagat cgacgcgctg caacgccaac agcggcggct
     5761 gaactggcaa cacctgtgac caagttggat gtattagctc atttgcaaaa tcaggaaaaa
     5821 cggatggcaa cggcagtccg aaatgttcta tctaagaaac aagaggcttt gaaaaaatgc
     5881 agtcagtctg ttatctttag acaacctgag cgcttgtatg acggttattt gcaacgcttg
     5941 gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc ggatttctga taacagacaa
     6001 ttagttcaag caagaactca tcaattagta caattatcac ctgttaccaa aatccaacgc
     6061 tatcaagacc gtttaggaca gttggacaag ctcttaggta gccaaatggc gttagtttat
     6121 gacgccaagg ttgctgagac caagcgactt tcggaagctt tgctcatgtt ggatactagc
     6181 cgaatcgtgg cgcgtggtta tgctattgta aaaaaagaag agtccgttgt agattcggtt
     6241 gagagtttga agaaaaaaga ccaagtaacg cttttgatgc gagatggtca agtagaatta
     6301 gaggttaaaa atgtcaaaac aaaagaaatt tgaggaaaat ctagcagaac tggaaaccat
     6361 tgtccaaagt ttggaaaatg gtgaaattgc tctggaagat gcgattactg cctttcaaaa
     6421 gggcatggtc ttgtcaaaag agctccaagc tacgctggac aaggctgaaa agaccttggt
     6481 caaggtcatg caagaagacg gaacagaaag tgattttgaa tgaaaaagca agaaaaatta
     6541 gctcttgttg agtcggcttt ggaagatttt tatggagacc agcagtttgc ctctagttta
     6601 cgggagtctg ttctctattc tattcatgct ggtggcaagc gtattcggcc ttttctcttg
     6661 ttagaagttc tggaagcctt gcaggttgcc atcaaacctg ctcacgcgca ggtagctact
     6721 gccttggaga tgattcatac agggagcttg attcacgatg accttcctgc tatggatgat
     6781 gacgattatc gaagagggcg gttaaccaat cacaagaaat tcggtgaagc tatggccatt
     6841 ttggctggag atgccttatt cttagaccca tatgccttga ttgcgcaggc agatttgcca
     6901 agtcagatca aggtggactt gattgccaac ttatcccttg cttcaggtag tctgggtatg
     6961 gtggcagggc aagttttgga tatggagggc gaacaccagc acttgtctct ggaagaactt
     7021 cagactattc atgccaataa gactgggaag ttactagcct atcccttcca agcggcagct
     7081 attatagctg aattgtcacc tgaaatgcag gtgaagctga aaactgtggg tgaattgatt
     7141 ggacttgctt ttcaagtcag agatgatgta ctggatgtga cagctagttt tgaggaaatc
     7201 ggcaagacac ctcaaaagga tctgcaggca gaaaaatcaa cctatcctgc cttgttgggc
     7261 ttggaagagt ccattgcctt ttgtaaccaa accctggatc aagctaatga aaaattagaa
     7321 gaaattgccc agcagcttcc ctttgaaaca gaatcgattg taagtgtagt agaaagtttg
     7381 agaatcaatg gctaaggaaa gagtggatgt actagcttat aaacaggggt tgtttgaaac
     7441 gagagagcag gccaagcgag gtgtgatggc tggcctagtc gtagcagtct ttaatggaga
     7501 acggtttgac aagccaggag agaaaattcc agatgacacc gaattaaaac tcaaggggga
     7561 gaaactcaag tatgtcagcc gtggtggttt gaaactggaa aaggccttgc aggtctttga
     7621 tttgtcggtg gatggcgcga ctacgattga tatcggggcc tctactggag gttttaccga
     7681 tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc gatgttggta ccaatcagtt
     7741 ggcttggaaa ttacgccaag acccacgagt tgtcagcatg gagcagttca attttcgcta
     7801 tgctgaaaag actgatttcg agcaggagcc gagctttgcc agtattgatg tgagtttcat
     7861 ttcccttagt ctgattttgc cagccttgca ccgtgtcttg gctgatcaag gtcaggtggt
     7921 agcacttgtc aaacctcagt ttgaggcagg acgtgagcag attgggaaaa atggaattat
     7981 tcgagatgct aagattcatc agaatgtcct tgaatctgta acagctatgg cagtagaggc
     8041 aggtttttca gtccttggct tggacttttc tcccatccaa ggtggacatg gaaatattga
     8101 atttttagcg tatttgaaaa aagaaaagtc agcaagcaat cagattcttg ctgagattaa
     8161 agaagcagta gagagggcgc atagtcaatt taaaaatgaa taaaaaagaa agacttgaaa
     8221 aaattagaag attggtgaca gattatcaaa tcggcacgca agaagaaatt gtagaacatt
     8281 tgaaagaagc aggtatcact gccactcagg cgacggtatc ccgagatatc aaagagttag
     8341 gtattgtcaa aattcctttg agagacaaca cctatgtcta cgaattacca aaatcaatcg
     8401 taaaaagtct gcaactggct gaagacaata tcgaatcggc tgaattgatg gataagatga
     8461 tcaatctcca agttattcca ggaaatacgg cttttgtaaa agctcagtta atcgatactt
     8521 ttgcagacaa gatttttagt tgtttgactg atgatagctc gattttagtc attgccagaa
     8581 gtggaagtct agcagaggaa atctttgaac aagtaaaaaa ttggtaggtc tatatgttac
     8641 ttgaaatttc gataaaaaac tttgccatta ttgaggctat ttccctcaat tttgaaaagg
     8701 ggatgactgt cctgactggt gaaacgggtg cagggaagtc gattatcatt gatgccatga
     8761 atatgatgtt gggagctcgt gcgacgacag atgttattcg tcatggtgca ccaaaggcag
     8821 agattgaggg gcttttctca gttgagaata gtcgcctttt acaggaaatt tttgatgagc
     8881 aaggtttaga attaggtgat gaaattatca tccgtcggga aatcttgcaa aatggtcgta
     8941 gtatcagccg tgtaaatggt cagatggtta atctgtctgt tttgcgagct attgggcaac
     9001 atcttgtaga tattcatggt cagcatgacc atgaggagtt aatgcgtccc caactgcata
     9061 tccagatgtt ggatgaattt ggtgatgccg ctttttggga cttgaaagaa acctatcaaa
     9121 cgagttttga tgcctatcgg aaaatgcgca agcaggttct ggaagtcaag aaaaaccaac
     9181 aggaacacaa ggcacgtatc gaaatgttgg aatttcaaat ggcagagatt gaggcagcaa
     9241 acttgcaggc tggagaagac ttggctctca atcaagagcg agataaactc ctcaaccaca
     9301 aaaatattgc ggatacactg accaatgcct acagtatgtt ggacaatgaa gatttttcaa
     9361 gtctggccaa tgttcgttca gctatgaatg acatggaaag tgtcgaagag tatgatcctg
     9421 aataccgtga aatttcaagc tctctgtctg agacctacta tgttttagaa gacattagca
     9481 aacgtttgga agctatcatt gaggaccttg attttgatgg caatcgcctg atgcaggttg
     9541 agaatcgttt ggacctcctt catactatta ctcgtaagta tggtgggact gttgatgatg
     9601 ttttgcttta ttttgccaag attacggaag aatacaatct cttgacaggc aataatcttt
     9661 cgtctgagga catggaagca gagcttaaga agttggaagt caatcttgtc aatttggcag
     9721 gtcaacttgc ttctgctcgt cataatttgg ctcagcaact cgaagctgag attaaacaag
     9781 aactgcaaga tctttatatg gaaaaagccc agtttcaggt tcgttttagt aagggaaaat
     9841 tcagtcgtga gggaaatgaa atggttgagt tttatatttc aaccaaccct ggagaagact
     9901 ttaaaccctt ggttaaggtt gcttctggag gggaattatc tcgtctcatg ttagccatta
     9961 agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt ctttgatgag gtggatacgg
    10021 gagtttcagg tcgtgttgct caagctattg ctcagaaaat tcataaaatt ggtcagcatg
    10081 gtcaggtttt ggctatctcc catttgccac aagtaattgc gattgcagat tatcaattct
    10141 ttattgagaa gattagtaat gaccattcaa cggtttcgac tgttcgtctc ttgacggtcg
    10201 aagagcgagt ggaggaagtt gccaagatgt tggcaggtga tgatgtgaca gaagcagccc
    10261 tgacgcaagc cagagaattg ttgagaaaca gggagaaata agatgacaga ttattatgta
    10321 attggagatg ttcacggaaa agctgggatg ctggaagacc ttctcaaaac ctgggatggt
    10381 cagccccagt tgctctttct aggtgatttg attgacagag gtgaggatag tcaccgtgtt
    10441 cttgaaatgg tcaaggactt ggtggacaat caaggggcta tctgtttgtc cggaaaccac
    10501 gagtatatgt ttttgacttg gctagatgac ccagaagaaa gttatgacca ttatcgtcgc
    10561 aatggtggag atacaaccat taactctatc ctaggtcgtc ccttggatgc accagttgat
    10621 ggagtagaag atgccaagcg ggttgccgct gaagcgtcag acttggtcga attcattcgt
    10681 caaatgccat ttgtagtaga gacagacaag tatatatttg ttcacgcagg tattgatttg
    10741 actttggacg actggcatga aacaacagat tataaaaaag tatggcttag aaaaccattc
    10801 cacggagctg aaaatcatac tggaaaaacc attgtcttcg gtcatacacc ggtctatggt
    10861 ttgttacagc aagaacgcgg tacatctgag ctttggacta cagaagatgg caagattgga
    10921 atggatggag gagctgttta tggtggtgtc cttcacggga ttgtctttac agaccaagga
    10981 atgacagaac accactttat cgaaaatgac ggcgttgtcg ctgaagatta gtactcctag
    11041 cagggtatgg tcttgtcaaa atatcaaaaa caatttataa taaatagata ccctgaaagg
    11101 aagagaatca tgaacttaga agaattgaaa aaacgacagg ggaagatccg taacttctct
    11161 attatcgccc atattgacca cgggaaatca actctagcag accgcatttt ggaaaaaaca
    11221 gagacggttt caagtcgtga aatgcaggcc cagcttttgg atagcatgga tctagagcgg
    11281 gaacgtggaa ttactattaa gttgaatgcc atcgagttga attacactgc aaaagatggg
    11341 gaaacttata ttttccactt gattgacaca ccagggcacg ttgactttac ctatgaagtt
    11401 tcacgttcgc tagctgcctg tgagggtgct attttggtgg tcgatgcggc tcaaggaatt
    11461 gaggctcaaa ctcttgccaa cgtttatctg gctttggata atgatttgga aatcatgcct
    11521 atcattaata aaattgacct gccggctgca gatccggagc gcgtgcgtac agagattgaa
    11581 gatgtgattg gtttggatgc cagtgaagca gttttggctt ctgccaaggc tggtattggg
    11641 attgaagaaa tcctcgagca aattgtagaa aaagtaccag caccaacggg tgatgtgacg
    11701 gcgccactta aggccttgat tttcgactct gtttacgatg cttaccgtgg ggttatcctc
    11761 caagtgcgtg tcatggacgg agtggtcaaa cctggtgata agattcagct catgagcaat
    11821 agtaagacct ttgatgtggc cgaagtcggt atttttacac caaaagcggt tggtcgtgat
    11881 ttccttgcga ctggtgatgt tggttacatt gcggcgtcta tcaagacggt tcaggatact
    11941 cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg cagaaccatt acatggttat
    12001 aagcagatga atcctatggt ctttgcgggt ctctacccaa tcgaatcaaa caagtacaat
    12061 gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg ctagtcttca gtttgaacca
    12121 gaaacatctc aggcacttgg atttggtttc cgttgtggat ttcttggact tctccatatg
    12181 gatgttattc aggaacgttt agagcgtgag ttcaatattg acctcatcat gacagctccg
    12241 tctgttattt acaaagttaa tttgaccgac ggtgagtcta tggatgtgtc taacccatct
    12301 gagtttccag acccaactaa gattgcgacc attgaagagc cttatgtcaa ggcgcaaatc
    12361 atggtaccac aggagttcgt cggagcagta atggagctag ctcagcgtaa gcgtggggac
    12421 tttgtgacta tggactatat tgatgataac cgtgtcaatg ttatctatca aattcctctt
    12481 gctgaaattg tctttgactt ctttgataaa cttaagtctt cgacacgtgg ttatgcaagc
    12541 tttgactacg aattgtcaga atatcgccca tctaagctgg tgaaaatgga tattcttctc
    12601 aatggagata aggtggatgc cctcagcttt atcgttcaca aggactttgc ctacgaacgt
    12661 gggaaactca tcgttgataa actcaagaaa atcatccctc gccaacaatt tgaagttcca
    12721 atccaagcgg ctattggaca caagattgtc gctcgtactg atatcaaggc ccttcgtaag
    12781 aacgtacttg ctaaatgtta tggtggtgac gtttctcgta agcgtaaact ccttgaaaaa
    12841 caaaaagctg gtaagaaacg catgaaatcc atcggatcag ttgaagttcc gcaagaagcc
    12901 ttcctcagcg tcttgagtat ggatgaagaa taagttatca gaaatgcctt tccttgttca
    12961 caggaaattt atataagaat caatagtaaa gcaggcttag atggtctgct tttttagttt
    13021 ttatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtatggtta
    13081 ctgacttcgt cagtttcatc tacaacctca aaatcatgtt ttgagctgac ttcgtcagtt
    13141 tcatctacaa cctcaaaact gtgttttgag
//
LOCUS       DAVKZB010000048        12893 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKZB010000048 DAVKZB010000000
VERSION     DAVKZB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.048"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..584)
                     /locus_tag="VXA80_001206"
     CDS             complement(<1..584)
                     /locus_tag="VXA80_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEV0353704.1"
                     /translation="MLNEKRKNYVEDKRQNFPSLLNWKMDLFSEEEKRNYFQTTCEIS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIMDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVNDIHKLGYKIVDIAPTNFIYTKNVLTP"
     gene            complement(597..1046)
                     /locus_tag="VXA80_001207"
                     /pseudo
     CDS             complement(597..1046)
                     /locus_tag="VXA80_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
     gene            1595..2272
                     /locus_tag="VXA80_001208"
     CDS             1595..2272
                     /locus_tag="VXA80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV0353705.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2259..3056
                     /locus_tag="VXA80_001209"
     CDS             2259..3056
                     /locus_tag="VXA80_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV0353706.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            3073..4176
                     /locus_tag="VXA80_001210"
     CDS             3073..4176
                     /locus_tag="VXA80_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV0353707.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEVSHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            4183..4374
                     /locus_tag="VXA80_001211"
     CDS             4183..4374
                     /locus_tag="VXA80_001211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353708.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            4477..5496
                     /gene="galE"
                     /locus_tag="VXA80_001212"
     CDS             4477..5496
                     /gene="galE"
                     /locus_tag="VXA80_001212"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0353709.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5498..6463
                     /locus_tag="VXA80_001213"
     CDS             5498..6463
                     /locus_tag="VXA80_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0353710.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(6514..6720)
                     /locus_tag="VXA80_001214"
     CDS             complement(6514..6720)
                     /locus_tag="VXA80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV0353711.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            6772..7248
                     /locus_tag="VXA80_001215"
     CDS             6772..7248
                     /locus_tag="VXA80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV0353712.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7257..7928
                     /locus_tag="VXA80_001216"
     CDS             7257..7928
                     /locus_tag="VXA80_001216"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV0353713.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8059..8397
                     /locus_tag="VXA80_001217"
     CDS             8059..8397
                     /locus_tag="VXA80_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV0353714.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            8400..9029
                     /locus_tag="VXA80_001218"
     CDS             8400..9029
                     /locus_tag="VXA80_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV0353715.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9269..10420
                     /locus_tag="VXA80_001219"
     CDS             9269..10420
                     /locus_tag="VXA80_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0353716.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            10738..11487
                     /gene="truA"
                     /locus_tag="VXA80_001220"
     CDS             10738..11487
                     /gene="truA"
                     /locus_tag="VXA80_001220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV0353717.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11477..12250
                     /locus_tag="VXA80_001221"
     CDS             11477..12250
                     /locus_tag="VXA80_001221"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0353718.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKSVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            12228..12689
                     /locus_tag="VXA80_001222"
     CDS             12228..12689
                     /locus_tag="VXA80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0353719.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLLDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         3685 a         2385 c         2842 g         3981 t
ORIGIN      
        1 ggggtcagta catttttggt atatataaaa tttgtaggag ctatatctac aattttatat
       61 cccagtttat ggatgtcatt tactatattc acaatgttat ccagtgtttt ttctctaata
      121 tttaaggagt gttctgtttc tcgaataaat tcttcaaagt ttaacccgtc aacttgttct
      181 tgaacaagaa aataatcatc catgatataa aattcatcag ttaaattagt agtataactt
      241 ttatcggcta atttttttag catgtgagct tcatttttta tatcatcaag agctgtccat
      301 tctccttcag catcataatt cacaaaaggt cttgactgct tgatgattac tttttgccca
      361 tccgattttc taatcgcccg ataaacattc cctttatttg atctcttaat aattttttcc
      421 attttgtatt tatttattgc agagtcttta cttgaaattt cacatgtggt ttgaaaataa
      481 ttcctttttt cttcttctga aaataaatcc attttccagt taaggagact agggaaattt
      541 tgtctcttat cttctacata gttcttcctt ttttcattta gcaataaata gctgaccttt
      601 ttattttttt catcataagc ctgttttttt aaaaaagcac tatatctata atgaactgga
      661 gaatgtagtc cacattgaaa atcagatagt atttttggag ccttaaattc tgacagttta
      721 ttcgtaagat tacaaatcat actcttggct tcagattctg acttagtata tagagttata
      781 aatttgttag cagtagggtt catttcccta ggggaattaa ttttttttaa ttcctctaaa
      841 tttttaacaa ctttaaagct acaattattt agttgggata gtttatacac aatcttaaaa
      901 atatttacag cgtcttttat ttgggagctt atgtgtattt tccacccttg tgcaggcacc
      961 ttatctgata aataatagta attccaaata tcaccctgtt ggaaattttt atcacatttt
     1021 aaacattctt tctctaataa atccataaca aactcctaat tattaaaata ggctagagac
     1081 acagcactag cctattccgt aagtaaagct acttatttta tataaaatat aactagcgac
     1141 ctgtaattaa acaagtaaaa gaacaatact tagggagacc tgtgacaatg atatcttcct
     1201 ctggaagtag ttctaattca aacaatgttt gttccatgac tgtatcctcc tgttatgata
     1261 aattccagaa agcatagagc aaattcgtgg ttcgtctgac taaatttctt atctgaacac
     1321 caccttcaaa tacaaatttt agtcaaactt ttattttttc taactttcta attatattct
     1381 accatgtgtt tttatatgcc aagaattttc tgaataaact gtttattttt tattttttga
     1441 catttttgca aattgtttaa aatctttcaa ttggattcac ttattgcttt aggacattga
     1501 aacttctttc gccagaggtg cttatattac tgggaagttg aagattgaaa tgtggtataa
     1561 taagaggtaa tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt
     1621 agcttccttt gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct
     1681 gcctatcgag ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga
     1741 aggtccctat cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca
     1801 agtccgttta gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac
     1861 cattgctggc atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt
     1921 agctaatgta gagcgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg
     1981 gctacaggat catggattcc agattgtagc agaaagcatc ttagaagaag ctggaaagtt
     2041 ttatgagatt ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt
     2101 tggtcccttc ttgtccaaag aagtcagtcc agtatttgtc caaaaatggc aaaaagaagc
     2161 tgagaagcta gagttcgccc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt
     2221 tctagtagat aagattcaag ctatcaagga ggtgctccat gttagcaagt gaagtgatcc
     2281 aagcttatga agccttttgc cctcaggaat tttctatgga gggagacagc tgtggtctgc
     2341 aaattggcac tttagacaag ggtatccaaa gggtcatggt ggctctcgat attcgtgaag
     2401 agacggtggc tgaagccatt gaaaagggtg tagacttgat tatcgtcaag cacgcgccta
     2461 tctttcgtcc tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc
     2521 tgattaagca tgacatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg
     2581 ggctcaatga ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa
     2641 caggtccaga acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat
     2701 tggcccaaca ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag
     2761 aggatgattt gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt
     2821 tctataagga tgctttggca aagggggcag atgtctatat cactggtgat atctactacc
     2881 atactgctca ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg
     2941 aagtgctttt tgtggaaaaa atcgcaacac tccttactca atggaaagag aaaaaaggct
     3001 gggaactaga gattttacct agtcaagcat cgaccaatcc tttccgtcat atctagttag
     3061 aaagtgaaga caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct
     3121 gcctactacc tctcgagaga aagtgaccta gaggtgaccg tttttgacca tgagcaaggt
     3181 caagccacca aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa
     3241 gcctggtaca agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta
     3301 gagaaatcag gacaagaaat cgacttttac cagcgttcgg gagtctttct cttgaaaaag
     3361 gatgaatcca atttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc
     3421 ttgatagggc aattagccat tctgaaccaa gcctcagcta atgaattatt ccctggtttg
     3481 cagggatttg accgcctgct ctatgcttct ggtggagcga gagtagatgg ccaactttta
     3541 gtgactcgtt tgctggaagt cagtcatgtc aagctggtca aagaaaaagt gactctgaca
     3601 ccgttagcat caggctacca gattggtgaa gaggagtttg agcaggttat tttggcgacg
     3661 ggagcttggt tgggggacat gttagagcct ttaggttatg aagtggatgt ccgtcctcaa
     3721 aaaggacaac tacgagatta tcagcttgcc caagacatgg aagattaccc tgttgtcatg
     3781 ccagaagggg agtgggattt gattcccttt gcaggtggga aattatcctt aggcgctacc
     3841 cacgaaaatg acatgggatt tgatttgacg gtagatgaaa ccttgctcca acaaatggag
     3901 gaggccacct tgactcacta tctgattttg gctgaagcta cttcaaaatc tgagcgtgtt
     3961 ggaatccgtg cctacaccag tgatttctct cctttctttg ggcaggtgcc tgacttaact
     4021 ggtgtctatg cagccagtgg actaggttca tcaggcctca caactggtcc tattattggt
     4081 taccatttag ctcaactgat ccaagacaag gagttgacct tggaccctga aaattaccca
     4141 attgaaaact atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa
     4201 actaggaaac tagccgcggg ttactcaaag cactgttttg aggttgtgga tagaactgac
     4261 gaagtcagct caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagtaaccat
     4321 acccatggca aggcgacgtt gacgtggttt gaattggatt ttcgaagagt ataagcattt
     4381 tactgaaatt ttatcaggta gtttaggatg gcaaatgaca ttccctgtca aaaatggtaa
     4441 aataagaaaa aataatctga gaatcgagga aaaaagatgc aagaaaagat tttggtaact
     4501 ggtggtgccg gttttattgg aacccacact gttattgaat tgatccaagc aggccatcaa
     4561 gttgttgtgg tggataatct tgtcaatagc aatcgtaaga gtttagaagt tgttgaaaga
     4621 atcacaggag ttgagattcc tttctatgag gcagatattc gtgacacaga taccctcaga
     4681 gatattttca agcaggaaga accgactggt gtcattcact ttgctggttt gaaggctgtt
     4741 ggcgaatcaa cacgtatccc tcttgcctac tatgacaaca atatcgctgg aactgtcagc
     4801 cttttgaaag ccatggaaga aaacaactgt aaaaacatca tcttcagttc ttctgcgaca
     4861 gtttacgggg atccgcacac agtgcccatc ttggaagatt tcccactttc agtgaccaac
     4921 ccatacggtc gtactaagct catgctagag gaaattttga ctgatattta caaagcagac
     4981 tcagaatgga atgttgtctt gcttcgttac tttaacccaa tcggagccca tgagagtggt
     5041 gatttgggag aaaatccaaa cggtattcca aacaatctct tgccatatgt gactcaagta
     5101 gccgttggaa aattagagca agtgcaagtg tttggagacg attacgatac ggaagatgga
     5161 acaggtgttc gtgactatat ccacgttgtc gatttggcta agggtcacgt tgcagctttg
     5221 aaaaaaatcc aaaaaggttc aggactaaac gtttataacc ttggaactgg taaaggttac
     5281 tcagttcttg aaattatcca aaacatggaa aaagcggtgg gacgtcctat tccttaccgc
     5341 atcgtagaac gtcgcccagg tgatatcgct gcctgctact cagacccagc aaaagctaaa
     5401 gcagaactcg gttgggaagc agaactcgac atcacccaaa tgtgtgaaga cgcatggcgt
     5461 tggcaaagca agcatccaaa tggatttgaa gactaagatg atgatttcaa tcatcgtccc
     5521 ttgtttaaac gaagaggaag tacttcctct tttttatcag gctctggaag ctttacttcc
     5581 agatttggaa acagaaatcg agtatgtctt tgtcgatgat ggatcaagtg atgggacctt
     5641 ggaactctta aaggcctatc gggagcaaaa tccggcagtc cattatattt ctttctctcg
     5701 aaattttggc aaagaagcag ccctttatgc aggcttgcaa tatgcgacag gagatttggt
     5761 ggtggtgatg gatgcagacc tccaagatcc tcctagtatg ttgtttgaga tgaaaaatgt
     5821 actagacaaa aatgtagact tggactgcgt tgggacacgg agaactagtc gggagggaga
     5881 acccttcttt cgcagtttct gtgctgttct cttttatcgc ctcatgcaaa aaatcagccc
     5941 agtagctctg ccgtcgggtg tccgtgattt tcgtatgatg agaaggtctg tagtcgatgc
     6001 cattttaagc ttgaccgagt ccaatcgttt ttcaaagggg ctcttcgcct gggtgggttt
     6061 caaaacctac tatttggact atccaaatgt cgaaaggcag gctggcaaga caagttggag
     6121 ttttaggcaa ctctttttct actccattga agggattatt aatttttcag attttccctt
     6181 gattatagcc tttgtagctg gtctcctatc ttgttttctt tctctgctga tgaccttttt
     6241 tgttgtggtt cggaccctca ttttgggcaa tccgacatct ggttggacct ctctgatggc
     6301 tgttattctc tttcttggag gcattcaact cttgaccatt gggattctcg gcaagtatat
     6361 cagtaagatt tatttagaga ctaaaaaaag accactttat cttatcaaag aaaaaagtga
     6421 ccttcctgat tttacagaaa aaaataaagt gaaaagacta taattttaca tggaaatgtg
     6481 ctaaactaga gggagtggat tatcgccatt gatttaggct tcctggtttc caatcagggc
     6541 gcgagttggg caatttttaa cagcctctaa gacatcctga ctaggagaaa tttctttttc
     6601 cagttggtca gggtcatcgt aaaaacgcac gattccatta tcgtggtaat caaataaatc
     6661 agaataagtt tggcaaagcc cacaggcgat acatcgttca ggtataagtg tgattttcat
     6721 gttcatattt atattgtaat aagaaaagta aaaaaaaaca aggagtaagg tatggcaaaa
     6781 gaaccgtggc aagaagatat ctatgatcaa gaagaatcaa gagcagagcg tcggcatcga
     6841 aaccacggag gggctgatag gatggctaat cgtattttga cgatcctagc tagtattttc
     6901 tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg gggaagtaat
     6961 cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca aatctcatct
     7021 tcaagcagtt ctcatcctga gcagagttca gagccagaat ctacttctag tagttcagaa
     7081 gaagctgcta atcctgaagg aacgattaaa gttctcgcag gagaagggga agcagctatt
     7141 gccgctcgtg caggaatctc cattgctcag ttagaggcct tgaatcctgg gcacatggct
     7201 acaggatctt ggtttgctaa tccaggtgat gttataaaaa taaaatagga gccgatatga
     7261 aaacaattca aattgctatt gatggtcctg cttccagcgg taagagtacg gtcgcaaaga
     7321 ttattgctaa ggattttgga ttcacctacc ttgatacagg agctatgtat cgtgcagcga
     7381 cctatatggc tcttaagaac caattaggag ttgaagaagt cgaagctctt ctagccttgt
     7441 tggaccagca tccaatcagc tttggacgtt cagaaactgg agaccagctt gtttttgtag
     7501 gagatgtgga tattacccat cctatccgtg aaaatgaagt gaccaatcat gtttctgcta
     7561 ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa attgcccagc
     7621 aaggcgggat tgtcatggat ggtcgcgata ttggaactgt tgtattgccg caagcagaat
     7681 tgaaaatttt cctagtagct tctgttgatg agagagcaga gcgtcgttac aaggaaaata
     7741 ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct gcgcgtgact
     7801 acaaggatag tcatcgtgag acttctcctc tcaaacaagc agaggatgct gtctaccttg
     7861 atacaactgg tttgaacatt caagaagtag ttgaaaaaat caaagcagaa gctgaaaaaa
     7921 gaatgtaaat gtagaaaagc cgatagattg atatcggttt ttttctaaat ttgtcaaaat
     7981 gctgatttta gggtataata gttgctgttc gagaaatttt gattttcaaa gaatagtgaa
     8041 tgatgataag gagaaaccat gaacaattta ccaaattgtc caaaatgtaa ctcagagtat
     8101 gtctacgaag acggtgccct actggtttgc ccagagtgtg ctcatgagtg gaatcctgct
     8161 gaagttgcag aagtagaaga aggtcttgtc gctatcgatg ccaacggaaa taaattgact
     8221 gatggtgata cagtaactct catcaaggac ttgaaagtaa aaggtgcgcc aaaagatttg
     8281 aaacaaggga cgcgcgtgaa aaatatccgc atcgtagaag gcgaccacaa tatcgactgt
     8341 aagattgatg gttttggtgc catgaaactt aaatcagagt ttgtgaggaa gatttaagca
     8401 tgtcaaaaca ctataaactt gtattttata gccgtatctt cttgtttcta gcggctttta
     8461 cgggagttta tcttgaaatt actaggcatg gtggttttgg gatgcttctc tattacacgg
     8521 ttctgtccaa cctcttggta actattttta ccctttatct tctaaaggtt atgagccgtg
     8581 taggtgaaaa ttggcaaagg ccaagtctct tgcgcttaaa aggtggggtc accatgagta
     8641 tcatgattac ctgtgtgatt taccatttcc tcttggcgcc cattgcgact aatttctata
     8701 ccctagaaaa tttcctttgc cactatatcg ttcccatctg gtttttagcg gataccctct
     8761 tttttgacaa acagggtcaa tacaagattt gggatccagc agtgtggacc attttaccct
     8821 ttctgtatat gatgtttgct ctttttaatg gcttggttct aaaactcaat attccaaatg
     8881 ccaaggataa tcctttccct tacttctttt tgaatgtgaa caagggttgg aatgttgtgt
     8941 ttaagtggtg tctgattatc tttgttgcct atatggtagc aggatttatt ttctacttta
     9001 tcaagcaaat caagagaaag tcatcctaat actcttcgaa aatctcttca aaccacgtca
     9061 gtttccatct gcaacctcaa aacactgttt tgagcagcct gcggctagct tcctagtttg
     9121 ctctttgatt ttcattgagt ataagataca agggattcat tttcgaattt aagaaggagt
     9181 ctacaaacca atgaggcttc ttcttttctt gcttatttga taactatcaa aaaagtagaa
     9241 gattaagaga aatatagaaa gagatttcat gaaacaattt ttagaacggg ccagcatttt
     9301 ggctctctcc ctcgttttga ttacctcctt ttccatttcg agtgccctac cagccatgtt
     9361 tgactattat cagggttatt ctaaggaaca aattgagctc ttggtgagct tgccttcctt
     9421 tggaatcatg atgatgttac tgctaaatgg tttcttagaa aaaatatttc ctgagcgctt
     9481 acagattagt ttgggcttgc tgattttatc attgagcggt acagctccct tctggtacca
     9541 agcctatccc tttgtctttg gaacacggct tctctttggt ttgggtcttg ggatgatcaa
     9601 tgccaaggcc atttctatta tcagtgaacg ctaccaagga aaaaggcgaa ttcagatgtt
     9661 agggctacgc gcttctgcag aggtcgttgg agcttctctc attaccttgg ccgtcggtca
     9721 gttgttggcc tttggttgga cagctatctt tctagcctat agtgctggat ttttggtgct
     9781 gcccctttat ctgctctttg tcccttatgg aaaatcaaag aaagaagtca agaaaagagc
     9841 gaaggaagca agtcgtttaa ctcgagaaat gaaaggcttg atttttacct tagctatcga
     9901 agcggcagtt gtagtttgta ccaatacagc tattaccatc cgtattccaa gtttgatggt
     9961 ggaaagagga ttgggggatg cccagttatc tagttttgtt cttagtatca tgcagttgat
    10021 cgggattgtg gctggggtga gtttttcttt cttgatttct atctttaaag agaaactgct
    10081 cctctggtct ggtattacct ttggcttggg gcaaatcgtg attgccttgt cttcatcctt
    10141 gtgggtggta gtagcaggaa gtgttctggc tggatttgcc tatagtgtag tcttgacgac
    10201 ggtctttcaa cttgtctctg aacgaattcc agctaaactc ctcaatcaag caacttcatt
    10261 tgctgtatta ggctgtagtt tcggagcctt tacgacccca ttcgttctag gtgcaattgg
    10321 cttactaact cacaatggga tgttggtctt tagtatctta ggaggttggt tgattgtaat
    10381 ctctatcttt gtcatgtacc tacttcagaa gagagcttag gattgattcc taagtttttc
    10441 ttttgatact caatgaaaat caaagagcaa actatagttg attgagtttg gaatagtacg
    10501 ctgtagctgc taaaatattt ctagaaatta atttgacttt cctaatagag ttgttcatat
    10561 cttatttcaa tctactatag aaagctagcc gcaggttgct caaaatagtg ttttgaggtt
    10621 gcagatggaa actgacgtgg tttgaagaga ttttcgaaga gtatgagatt tttataccca
    10681 tacaatattt gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggctatg
    10741 acgagatata aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag
    10801 cctcatgcgc gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg
    10861 caaaccatta ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag
    10921 gtcattcatt ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat
    10981 acccagtctc ctgaagatat tgatgtgatt tcgattgagc ttgtggcaga tgattttcat
    11041 tgccgttatg ccaagcatag caagacctat gagtttattg tggatagagg acgtcccaaa
    11101 aatccgatgc gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg
    11161 cagattgcaa tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg
    11221 actagtgtag aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca
    11281 ggacaatttt tgacctttac cttttcagga aatggtttct tgtataaaca gattcgcaat
    11341 atggtgggga ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc
    11401 atcttggaga agaaggacag gcaacttgca ggtcccactg cagcaccaaa tggtttgtat
    11461 ttaaaggaga ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt
    11521 tagtggtggt ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt
    11581 tgtagcagtg acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga
    11641 tgataccatt tttcaacatg aattagatag cttgcgtgat gtggaatttg ggggaattaa
    11701 gattggtctt ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg
    11761 cccaggagta cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc
    11821 tgtcagtgag ctctgccaag agttgattcg ctttttccct tatgttagtg tgattacgcc
    11881 taatctccca gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa
    11941 aactgcagcg cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa
    12001 tcgtcttagt caggacaagt ctgtggatgt cttttatgat ggacagacct ttactatcct
    12061 agaaaatcca gttatccaag gccaaaatgc tggtgcaggt tgtacctttg cctctagcat
    12121 tgccagtcac ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt
    12181 cgtttatcgt gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa
    12241 aaacaactaa aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt
    12301 tcttaaaaat cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg
    12361 cggcctttta ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttgc
    12421 ttgacctctt atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc
    12481 agggattttt cgcaggattt aaaggaaaaa gtcagtggtt gggccttatt ttagcaacta
    12541 ttgccatggt agggggttac gccttgggtt ctactttgat gaatggctgg gcagcagccc
    12601 tcccagaaat tctaccaaat tttatgcaaa atatggtagg gatgattgta ggatttattc
    12661 ttagtcaaag tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca
    12721 gtttccatct gcaacctcaa aacactgttt tgagcagcct gaggctagct ttctagtctg
    12781 attttcattg aatataaaga ggttgagtaa aaagtctttc aaataagaaa aatgaactgc
    12841 cccccaaaag ttagacagaa aaaatctaac ttttggggtg ttttattatg aaa
//
LOCUS       DAVKZB010000049        12873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKZB010000049 DAVKZB010000000
VERSION     DAVKZB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.049"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..773)
                     /locus_tag="VXA80_001223"
     CDS             complement(<1..773)
                     /locus_tag="VXA80_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV0353720.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFER"
     gene            960..2681
                     /gene="pabB"
                     /locus_tag="VXA80_001224"
     CDS             960..2681
                     /gene="pabB"
                     /locus_tag="VXA80_001224"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV0353721.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            2682..3299
                     /locus_tag="VXA80_001225"
     CDS             2682..3299
                     /locus_tag="VXA80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0353722.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            3349..4339
                     /locus_tag="VXA80_001226"
                     /pseudo
     CDS             3349..4339
                     /locus_tag="VXA80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            4474..5433
                     /locus_tag="VXA80_001227"
     CDS             4474..5433
                     /locus_tag="VXA80_001227"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV0353723.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            5530..6369
                     /locus_tag="VXA80_001228"
     CDS             5530..6369
                     /locus_tag="VXA80_001228"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV0353724.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVFWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(6417..6587)
                     /locus_tag="VXA80_001229"
     CDS             complement(6417..6587)
                     /locus_tag="VXA80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV0353725.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            6708..7592
                     /gene="miaA"
                     /locus_tag="VXA80_001230"
     CDS             6708..7592
                     /gene="miaA"
                     /locus_tag="VXA80_001230"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV0353726.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            7585..8823
                     /gene="hflX"
                     /locus_tag="VXA80_001231"
     CDS             7585..8823
                     /gene="hflX"
                     /locus_tag="VXA80_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV0353727.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPIELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKTHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            8816..9439
                     /locus_tag="VXA80_001232"
     CDS             8816..9439
                     /locus_tag="VXA80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV0353728.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            9454..10383
                     /locus_tag="VXA80_001233"
     CDS             9454..10383
                     /locus_tag="VXA80_001233"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV0353729.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGVKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            10404..11159
                     /locus_tag="VXA80_001234"
     CDS             10404..11159
                     /locus_tag="VXA80_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV0353730.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(11213..12121)
                     /locus_tag="VXA80_001235"
     CDS             complement(11213..12121)
                     /locus_tag="VXA80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0353731.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            12185..12454
                     /locus_tag="VXA80_001236"
     CDS             12185..12454
                     /locus_tag="VXA80_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV0353732.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLKAEAVLRREHKVELDYIEKNGEKN"
     gene            12454..12834
                     /locus_tag="VXA80_001237"
     CDS             12454..12834
                     /locus_tag="VXA80_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0353733.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         3783 a         2455 c         2964 g         3671 t
ORIGIN      
        1 tttcgttcaa actgattcat aatttgaaca cgaaaacgac tcatggcacg gctgagatgt
       61 tggataatat ggaaacgatc tagaacgatt ttagcacacg gaaaaagctg tttagccaag
      121 tcatagtaag gactaaacat atccatcgta atgattttca cttgacaacg aacggctcta
      181 tcgtagcgaa gaaagtgatt tcggatgaca gcttgtgttc tgccttcaag aacagtgata
      241 atattaagat tatcaaaatc ttgcgcaatg aaactcatct ttcccttagt gaaggcatac
      301 tcatcccaag acataatctt tggaagccga gaaaaatcat gctcaaagtg aaagtcattg
      361 agcttgcgaa tgacagttga agttgaaatg gccagctgat gggcaatatc agtcatagaa
      421 attttttcaa ttaacttttg agcaattttt tggttgatga tacgagggat ttggtgattt
      481 ttctttacca ggggagtctc agcaaccatc atttttgaac agtgatagca cttgaaacgg
      541 cgttttctaa ggagaattct agaaggcata ccagttgttt cgaggtaagg gatcttagac
      601 ggtttttgaa agtcatattt cttcattaga cttccacaat cagggcaaga tggagcctca
      661 taatccagct tagcgataat ttctttgtgg gtatccatat tgatgatatc tagaatcttg
      721 atgtttgggt ctttaatatc gagcagtttt gtgataaaat gtaattgttc catatgaatc
      781 tttctaatga tggttttgtc gcttttcatt ataggtcata tgggactttt tttctacaat
      841 aaaataggct ccataatatc tatagtggat ttacccacta caaatattat agagccataa
      901 aatcgtaaaa aggtagatca gctttgtctg tatctgcctt tttatgctat acttaaggta
      961 tgcatagaaa aacagtgatt gattttagga ctttagggga gagatatacc tttacccagc
     1021 ctattaaaga gttgaaaacg agaaatgtag aagaagtggc agatttgctg gcacaagtgg
     1081 aaagctacca agagcaaggt tattatgtgg tggggtatgt cagctacgag gctgcacctg
     1141 cttttgagga aaaattagca gttcacaagg atcccctact gggagagtac ctgctttatt
     1201 ttactgttca tgatagcgta gaaacttccc ctattcctct gacttatgag gatattgatt
     1261 tgccttcaaa ttggcaggaa gtaacgtctg cagcagacta tgaaaaggcc attgcccaga
     1321 tacaccatca cttgaggcag ggagacacct atcaggtcaa ctacaccgtc caactcaagc
     1381 aaaagttaag tgccaatcct tttgccatct acaatcgtat ggtggtagag caggaggcgg
     1441 gctacaatgc ctatgtggaa catgacgaga tggcagtgat ttccatgagc ccagagctct
     1501 tttttgagca aaatgatcgc gagttgacaa cacgaccaat gaaggggacg actcagcgtg
     1561 gggtaactga ccaagaagat cttgaacagg ccagttggtt ggaacaggat cccaaaaatc
     1621 gctctgaaaa tatgatgatt gtggacctct tgcgcaatga tatgaaccgt atttctgaag
     1681 ttgggagcga gcacgtggag cgtctgtgtc aggtagagca gtattcaact gtttggcaga
     1741 tgacttcgac catcaagagt cagttgcgag aggatgtgga ccttgttgaa atcttccgct
     1801 cactctttcc ttgtggttcc ataacgggtg caccgaaaat tgcgacaatg gagattatca
     1861 aggacttgga gcctcaaccg cgtggagtct actgtggaac gattggtctc ttgcttccaa
     1921 atggacgacg gatttttaat gtggccattc gtaccattca acttcacaaa ggtcaagcca
     1981 tttatggagt tggcggaggg attacttggg atagcacatg ggaatctgaa taccgagagg
     2041 ttcatcaaaa ggcagcagtt ctttatcgta aacaagctcg cttccaactg attacgacag
     2101 gggaaatcag ccagaagaac ctgctgtttg aagatcaaca tctggaaaga ctgagaaaag
     2161 ctagtcgtta ttttgctttt ccttttgatg cagaagactt gggacacaag attgaggaag
     2221 agtgtcagga ttgtgaagct aatcgagatt accgcttgcg aatcagcctt agcaaatctg
     2281 gagagataga agtcaatcgt caagtattaa cccctctcag tacaagcttt tgtcaggccc
     2341 aagtctgtct tcaggaagct gctttgaatc aatcctttac ctactttaaa accactcacc
     2401 gaccgcattt gagcctagga gaacaagaga agatttacca caataagtca ggagaactgc
     2461 ttgaaacctc tataggaaat ttggttctga aaatcgctgg aaaactctac acaccgccta
     2521 tccgacttgg aatcttgcca ggaatttacc gtcagcattt gctagaaaca ggacaggtag
     2581 aagagaaagt cttgaccttg gcagatttaa cccaagcaga agctatttac ggctgtaatg
     2641 cagtgagagg cttgtatgaa ctaagccttg aggagaacta gatgaaattg atatttttgc
     2701 atgggttagg gcagtcagca gagtcttgga aagaagttcg gaatctactg acagattatc
     2761 cttctgaggc tatcgagtta tttccttctg gagttagtaa ctatcaacaa gctaaagagc
     2821 gagtttatca gcacctagct caagagacag aaccttttgt tttgattgga ttgtccttag
     2881 gtgctgccct gacactagaa ctttccagtt acgatttacc aaatcttcga gctttgattc
     2941 tgtcaggatg cccgcttaaa ttagcaggga atatcctatt ttatcttcag ttgctgattt
     3001 ttaaactact tcccaaaaga gtatttgaaa aacagggagc agacaagact cttatggtcg
     3061 gagtttctga ggaattgaag acacttgatt taacggatat agcagggact tgctcctatc
     3121 caaccttact aatttgtggc agcaaagata aaccgaatct tagttctatg aaagctctcc
     3181 atagactgtt aacagattcg cagtttcaga tcattcctga tggccctcat gtcttgaata
     3241 aggcaaaacc aaaggagttt gtagaaaaga ccagaagttt ccttgaattg ctgaaataag
     3301 tttgataaaa taatattgaa atcgatatca taattatggg agaaagaaat gaataaacgt
     3361 ctatttttaa aaatgagtct ggtgacgttg ccaattttag ccttgttttc acaaccagtt
     3421 ttggcggaag aaaacatcca tttttcgagc tgtaaggaag cttgggcgaa tggctattcg
     3481 gatattcacg agggagaacc tggttattct gccaagttag accgtgatca tgatggtgtg
     3541 gcttgcgaat tgaaaaatgc tcctaagggt gcttttaaag caaaacagtc aacggctatt
     3601 caaatcaaca caagttcagc aacaacaagt ggttgggtta agcaggacgg cgcttggtac
     3661 tactttgatg gaaatggaaa tctagtgaaa aatgcatggc agggaagcta ttacctgaaa
     3721 gctgatggta aagtggcaca gagtgaatgg atttatgact cttcttatca agcttggtat
     3781 tatttgacat cagatggttc ttatgcaaaa aatgcatggc aaggagctta ttaccttaaa
     3841 tcaaacggta aagtggcaca aggtgagtgg gtttatgatt cttcttacca agcatggtat
     3901 tacttgaaat cagatggttc atatgctcgc aatgcatggc aaggaaacta ctatttgaaa
     3961 tcagatggta aaatggctaa aggtgaatgg gtttatgatg ccacctatca agcttggtat
     4021 tatttgacat cagatggttc ttatgcttac agtacatggc aaggaaatta ctatctaaaa
     4081 tcggatggta aaatggctgt caatgaatgg gttgatggtg gacgttatta tgttggcgct
     4141 gacggagttt ggaaggaagg tcaagcaagt acagtttctt ctagtaatga tagcaatagt
     4201 gaatattctg ctgctttagg aaaggcaaaa agttataatt cgttattcca catgtcaaaa
     4261 aaaacgtatg tatagacaat taacttctga ttttgataaa ttttcaaatg atgcagctca
     4321 atatgccatt gatcattaga tgattaacag atactggtag gcgaaaaaat tccttataat
     4381 tcatttacgc aaacctttgc ataagtgaat ggattttgat atactagtac tgtaagcatt
     4441 ttcaattact acacaatgaa aaaggagaat atgatgagtc aaaagattat tgggattgac
     4501 cttggtggaa cttctatcaa atttgcaatc ttaacaacag caggagaaat ccaaggaaaa
     4561 tggtcaatca agaccaacat tttggatgag ggaagtcata tcgttgatga tatgattgag
     4621 tctattcaac atcgtttgga cttgcttgga ttggcagcag cggacttcca aggcattgga
     4681 atgggatcac caggtgtggt tgaccgtgac aaagggactg ttatcggtgc ctacaacttg
     4741 aactggaaaa cccttcaacc aatcaaacaa aagattgaaa aagccttggg cattccattt
     4801 ttcatcgata atgatgccaa cgtagcagct cttggtgagc gctggatggg tgctggagat
     4861 aaccaaccag acgttgtctt tatgacactc ggtactggtg ttggtggtgg tattgtcgca
     4921 gaaggcaaat tgcttcatgg tgttgctggt gcagcaggtg aacttggtca tatcactgtt
     4981 gattttgacc aaccaattgc atgtacttgt ggtaaaaaag gctgccttga gacagttgct
     5041 tcagcaacag ggattgtcaa cttgactcgt cgctatgccg atgaatacga aggcgatgca
     5101 gccttgaaac gcttgattga taacggagaa gaagtaactg ctaagactgt ctttgatctc
     5161 gcaaaagaag gagacgacct tgctttgatt gtttaccgta acttctcacg ttacttggga
     5221 atcgcttgtg ctaacattgg ttcaatccta aacccatcaa caatcgtcat cggtggtggt
     5281 gtgtcagctg cgggagaatt ccttctacaa ggtgttcaaa aagtttacga tgaaaatagt
     5341 ttcccacaag tacgaacatc tactaaatta gctcttgcaa ctctaggaaa tgacgctgga
     5401 gttatcggag cagcatcact tgtattgcaa taagataatg agagaggaaa atactaacct
     5461 agagttagta atgttcctct tttatttttt atgctatact agttaggaca atgaatgagg
     5521 tgagagtaaa tgacaaaagc agatacgatt tttaaagaga atattgaacg cattctaaag
     5581 gaaggtgttt tctcagagca ggctcgtcct aagtacaagg atgggactgt tgctaattcc
     5641 aagtatgtaa ctggtgcctt ttccgagtac gacttatcta aaggagaatt tcctatcaca
     5701 accttgcgtc ccattgcaat caaatccgcc atcaaagaag ttttctggat ttaccaagac
     5761 cagtcaaata gcttagaagt gcttaatgac aagtacaatg ttcactactg gaatgactgg
     5821 gaagttggag acacgggaac cattggtgag cgctatggtg ccgttgttaa gaaacacgac
     5881 attatcaata agcttctcaa acagttggaa accaatcctt ggaaccgccg caatattatt
     5941 tcgctctggg attaccaagc tttcgaagaa acagatgggc tgctcccgtg cgcctttcag
     6001 accatgtttg atgtccggcg tgttgatggg gaaatctatc tggatgcgac cttgacccag
     6061 cgctccaatg atatgctggt ggcccaccac atcaacgcta tgcagtatgt ggctttgcag
     6121 atgatgattg ccaaacattt tggctggaag gttgggaagt tcttctactt catcaacaac
     6181 ctccatatct atgataatca atttgaacaa gctcaggaat tgctccgtcg ggagccgtca
     6241 aactgccaac cacgcttggt tttaaatgtt cctgatggga ctaatttctt tgatatcaaa
     6301 gcagaagatt ttgagttggt ggattatgac cctgttaagc cacagttgaa gtttgaccta
     6361 gctatttaaa agaatagaaa aaagaagttg agaataatcc caacttcttt tgtttcttaa
     6421 cgtgatacgc ggcgacgagc tgctttttta cggttttctt cgatgaaagc tgctttttgc
     6481 tcttctggtt cgattacttt ctttttaaat gcgtatactg cacctgcaac ggcagcgaca
     6541 gttcctgcga cacctgttac aagaccttta gcgaatcctt tagccatgag tcttcctcct
     6601 ttatattctc aatcagccag cctcctcaag aggtcacatt tttctgactg acctttttgt
     6661 gttataataa tagtaacgaa aaaatgggaa tttttcaagg aaaaaagatg aaaacaaaaa
     6721 taattgtgat tgttggaccg actgctgttg gaaagacggc tctagctatt gaagttgcaa
     6781 agcgctttaa tggcgaagtg gttagtggag atagccagca agtctatcga ggacttgata
     6841 ttgggacggc caaggctagt ccagaagagc aggcagctgt tcctcatcat ttaatcgatg
     6901 ttagagagat aaccgagtct tactcggctt ttgattttgt ttcagaagct aagatgacta
     6961 ttgaggatat tcacagccgt ggcaagctag ccattatcgc cggtgggact ggactttata
     7021 tccagagctt gctagaaggt taccacctag gtggggagac tcctcatgag gagattttgg
     7081 cttatcgagc tagtttggag ccatattcag atgaggaatt agcccatttg gtggagcaag
     7141 caggccttga gattccccag tttaatcgtc gtcgtgctat gcgtgcctta gaaattgccc
     7201 attttggtca ggatttggaa aatcaagaga ccttgtatga accgctgatt atctgcttgg
     7261 atgatgaacg tagtcaactt tatgagcgta tcaaccaccg agtggatttg atgtttgagg
     7321 ctgggctttt ggatgaggcc aagtggctgt ttgaccattc ccctaatgta caggctgcta
     7381 aaggaattgg ctataaggaa ctctttcctt atttccgtgg agagcagacc ttggaggaag
     7441 ctagtgagag tcttaaacag gcgactcgtc gttttgccaa gcgtcagctg acctggttcc
     7501 gtaatcgcat gcaggtcacc ttttatcaga ttggagaatc tggtgtacaa gaccgcattt
     7561 taagccagat agaggagttt ttagatgatt gaaacggaga aaaaagagga gcgagtcctg
     7621 ctgattggtg tggaattgca gggtatggac agttttgacc tctccatgga agaattggct
     7681 agtttagcga aaacggcagg ggcagtcgtt gtagatagct acagacaaaa acgtgaaaaa
     7741 tatgattcca agaccttcgt cggctctggt aagttggaag agattgcgct tatggtggat
     7801 gcagaagaaa tcactactgt catcgtcaac aatcgtctga ccccaaggca gaatgtcaat
     7861 ctagaggaag ttctcggtgt taaggtcatt gaccgtatgc agttgatttt ggatatcttt
     7921 gccatgcggg ctcgaagcca tgaagggaag ctccaagtcc acctagctca actcaaatac
     7981 ctcttgcctc gcttggttgg tcaggggatt atgctcagcc gtcaggcagg gggaattggt
     8041 tcccgtggtc ctggtgaaag ccaactggag ctgaaccgtc gtagcgttcg caatcaaatc
     8101 acggatatcg agcgccagct caaggtggtt gagaaaaatc gtgcgactgt cagagaaaaa
     8161 cgtttggagt ctagcacttt taagattggt ttgattggtt ataccaatgc tgggaaatca
     8221 actatcatga acatcttgac cagtaagacc cagtatgaag cagatgagct ctttgcgact
     8281 ctggatgcga caaccaagag tattcatctg ggaggcaacc tccaagtaac tttgacagat
     8341 accgttggct ttatccaaga tttgccgata gagttggtgt ccagtttcaa gtcaaccttg
     8401 gaagaaagca agcatgtgga ccttctggtt catgttatcg atgctagcaa tccttaccac
     8461 gaggaacatg aaaaaacggt tctctccatc atgaaagacc tggacatgga agatattcct
     8521 cacttgacgc tttataataa agcggatttg gtagaggatt tcacgcctac ccaaacgcca
     8581 tataccctca tttctgccaa gtctgaggac agtcgtgaaa acttgcaagc attattgcta
     8641 gataagatta aggaaatttt tgaagcattt accctgcgag tgcctttttc aaagtcctac
     8701 aagactcatg atttagagag tgttgcaatt ctggaagaac gtgattatca ggaagacggc
     8761 gaagtgatta caggctacat ttccgagaaa aataaatgga ggttagaaga attttatgac
     8821 tgatattaaa actttggctc taaagtatgg aggttataca agtctggaca aggtctatct
     8881 ggatcagctt ctagctggta agacagagca ggagcagtta acacttatta cacctccgcc
     8941 aagtgttgtt aatgcctact ttgcagaact ctatcagaaa aaaagtcctc aggctgcgac
     9001 ggattatttt gcagaactca gtcaggaact gaatctttac aatactgagc caagtttcac
     9061 cctagaaaat aagtctttta ttcggcttaa tctgtctggt aaatcctttg gtttttgtta
     9121 tgagagtgac ggctttggtc ggattttctc tgaaaatgag gagaaaatct cggatgactt
     9181 gctctttgaa attgcgcaaa ttttccccca tcaactagtc tttgaagagt ctggtaagat
     9241 ttacatgaag cctgtcgagg acgaggaagt tgtcagccta gaaagtctca cagctttgac
     9301 tgatttggaa agattggctg atggtcgaaa acgtctcaaa ggctacagcc aagaggattt
     9361 attgcaagaa gctgctgctt tttctggcaa acgctatttc cgatcggaaa accgcacagc
     9421 catgttatat attgattaat tagaaagtat cgaatggata ttcaattttt aggaacgggg
     9481 gctggtcagc cctctaaagc tcgcaacgtt tcaagtctcg ccctgaaact tttggacgag
     9541 attaacgaag tttggctctt tgactgtgga gaaggtacgc aaaatcgcat tctggaaacc
     9601 acaattcgac cacgtaaggt cagcaaaatc tttattaccc atctgcatgg agaccacatt
     9661 tttggtttgc caggtttcct ttctagccgt gcctttcagg ccaatgaaga gcagacagat
     9721 ttggaaatct acggacctca aggaatcaag tcatttgtct taaccagcct tcgtgtgtca
     9781 ggttctcgtc tgccctaccg cattcatttc catgagtttg accaagattc tctgggtaaa
     9841 attcttgaaa ccgataaatt cactgtgtat gcagaggagc tggaccacac tattttctgt
     9901 gttggctatc gtgtcatgca aaaggatcta gaagggacgc tggatgctga aaaacttaag
     9961 gctgctggtg ttccgttcgg cccgcttttt ggtaaaatca aaaacggcca ggatcttgtt
    10021 ttggaagacg gaactgaaat caaggcagca gactatatct cagcgccacg tccaggtaag
    10081 attatcacta ttttaggaga tactcgaaaa acggatgcca gtgtgcgtct ggctgtcaat
    10141 gcagatgtcc tagttcatga gtccacttat ggcaagggtg atgaaaaaat tgctcgtaac
    10201 catggtcact caactaatat gcaagctgca caagtagcgg tagaagcagg tgtcaaacgc
    10261 ctcctactca accatatcag tgcccgtttc ctctcaaaag atattagcaa actcaagaaa
    10321 gacgctgcca caatttttga aaatgtccat gtggtcaaag acttggaaga agtggaaatc
    10381 tagcagtcac agaaaggata agtatgccta ctattctcat taccggagct agcggtggtc
    10441 tagctcaaga aatggtcaaa ctcctgccca atgaccaact catcttgctt ggtagaaata
    10501 aggaaaaatt agcccaactc tacggaaatt attcccatgc agaattgatt gaaattgata
    10561 ttaccgacga ttcagcccta gaagctctgg taactgatct ttatctccgc tatggcaaga
    10621 ttgatgtctt gattaacaac gctggttacg ggatttttga gggatttgac caaattgctg
    10681 ataaagacat tcaccagatg tttgaggtca atacctttgc cctgatgaat ctgtctcgtc
    10741 accttgcggc tcgtatgaag gaaagcagca aagggcatat catcaacatc gtcagcatgg
    10801 caggtctaat agctactggc aagtctagtc tttactcagc gaccaagttt gcggctattg
    10861 gtttttcaaa tgctctgcga ctcgaactta tgccctatgg agtctatgtg acaacagtca
    10921 atccaggtcc aatccgaaca ggattttttg accaagctga cccagatgga acttatctta
    10981 aatcggttga ccgcttcctc ttagaggcag atgcagtggc taaaaagatt gtcaagatta
    11041 taggaaaaaa taaacgagaa ctcaatctcc cgattttgtt gaacctagcc cataagtttt
    11101 atactctctt tcccaagcta gctgataagt tggcagggga aacttttaat tataagtaaa
    11161 aagaaccaat gtgcaggttg ttgctagcct acatattggt tcttatcatt tctcaactat
    11221 caaactgaac ttcttctagt agatagtcga taaagcgttc gcccatctta gataggctgg
    11281 ttttctcatg ctggatatag accagctcga tcgggtcatc aatatccagt gggatagaaa
    11341 cgatattgtc tccgtttagg ttgctgttca aaatccctgt cgcaatggta taaccatcca
    11401 aaccaatcaa gagattaaag agggtggcac ggtcactgac cacaatggat ttcttgtggt
    11461 gttcttgaga aagaatctct tctgaaaagt agaaggagtt gtgcgtccct tggtcatagc
    11521 tgaggtaagg gaaattctcc aaatcagaca gtttcacctt gtctttcttt gccagagggt
    11581 tggtcttgct gacaaagata tgcggttgcg ctgtgaagag atggtgggct agcaggtgat
    11641 tgtcatccag catcttggtt aaaacatcac ggttgtaact gtttaagaag aggaccccga
    11701 cctcactgcg gaagttcttg acgtcgtcga taatctccca agtccgagtt tcacgaagga
    11761 agagttcgta tttctccata tcgcttttct tgagcaaaga gacaaaggca ttgaccacaa
    11821 aggcatagtg ttgagacgaa acgctaaata gttcgcggtg ggcgacagga tttttatagc
    11881 gttcctccag aagctgggtc tgctcgacaa cctgacgggc ataagagaga aactccatcc
    11941 catcacgggt caaggtgatt cccttgggat tgcggataaa gatctcaatg cccatttcat
    12001 tttccaaatc tcgcactgca ttggagagac ttggctgagt gataaagagt tgcttggctg
    12061 cctcattcat ggagccagtt tcgacgattt tgataatata atgtaattgt tgaattctca
    12121 tgtttttatt gtaccatact tgccttagaa tcgctaaaga agataggaaa ataaggagca
    12181 ttgtgtgagt ctcttcttga aaaaaacaca aaattttgct agaatgagtc ttatgaaaac
    12241 attctatgat gtgcagcaat tcctcaaacg atttggtatt attgtttaca tgggaaaacg
    12301 cttatatgat attgaactga tgaagttgga actatctcgg atttacgatg cagggttgat
    12361 ggataaatta gactatctaa aggcagaagc agttcttcgc agagagcaca aggtagaatt
    12421 ggattatatt gagaaaaatg gagaaaagaa ctaatggtta cttggatttt gtgggcactt
    12481 atactagcaa tgttggcgtg gatgggcttt aactatcttc gtattcgccg tgcggctaaa
    12541 attgtggaca atgaggagtt tgaagccttg attcgtacgg gtcaattgat tgatttgcgc
    12601 gacccagcag aattccacag aaaacatatc cttggtgcac gcaatattcc ttcaagtcag
    12661 ttgaaaacta gtcttgcagc ccttcgtaaa gataaacctg tccttctcta cgaaaaccaa
    12721 cgtgcgcaac gagttacaaa tgcagctctt tacttgaaaa aacaaggttt ttctgagatt
    12781 tatatccttt cttatggctt ggattcttgg aaagggaaag tgaagactag ctaaccttat
    12841 ttccaaatta gtcgaatgat aggatgaagc tat
//
LOCUS       DAVKZB010000050        12700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKZB010000050 DAVKZB010000000
VERSION     DAVKZB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.050"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            55..390
                     /locus_tag="VXA80_001238"
     CDS             55..390
                     /locus_tag="VXA80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353734.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGGRH"
     gene            575..1366
                     /locus_tag="VXA80_001239"
     CDS             575..1366
                     /locus_tag="VXA80_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353735.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSIFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1359..2000
                     /locus_tag="VXA80_001240"
     CDS             1359..2000
                     /locus_tag="VXA80_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353736.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            2004..2636
                     /locus_tag="VXA80_001241"
     CDS             2004..2636
                     /locus_tag="VXA80_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0353737.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2664..2951
                     /locus_tag="VXA80_001242"
     CDS             2664..2951
                     /locus_tag="VXA80_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353738.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLVFGILSILMAFLSKKVSLFVFGLICCLSLWINLFILFALLPIFGN"
     gene            3097..5610
                     /gene="secA"
                     /locus_tag="VXA80_001243"
     CDS             3097..5610
                     /gene="secA"
                     /locus_tag="VXA80_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV0353739.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5691..6722
                     /locus_tag="VXA80_001244"
     CDS             5691..6722
                     /locus_tag="VXA80_001244"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0353740.1"
                     /translation="MVFAAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6724..7755
                     /locus_tag="VXA80_001245"
     CDS             6724..7755
                     /locus_tag="VXA80_001245"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0353741.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYATLTK"
     gene            7794..8162
                     /locus_tag="VXA80_001246"
     CDS             7794..8162
                     /locus_tag="VXA80_001246"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV0353742.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8152..9255
                     /locus_tag="VXA80_001247"
     CDS             8152..9255
                     /locus_tag="VXA80_001247"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV0353743.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREVSEVEQAQDLLQKHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9274..11289
                     /gene="recG"
                     /locus_tag="VXA80_001248"
     CDS             9274..11289
                     /gene="recG"
                     /locus_tag="VXA80_001248"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV0353744.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11605..12585
                     /locus_tag="VXA80_001249"
     CDS             11605..12585
                     /locus_tag="VXA80_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV0353745.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTPPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3671 a         2386 c         2880 g         3763 t
ORIGIN      
        1 ataacattta atagaacgaa actaaagaaa acaatttaaa agaggaggtt tgtgatggaa
       61 cacttgttta aaatgataat cttattaccc tgtttttact ttttcagctg gattgacaag
      121 gataatagag aaagtaaatt tttcccaatt ttttactatt tttactggat ttacatcacc
      181 ctctatgctc tttttagcct tgcttggaca gttttttcag ttctgttttt caatatcgtc
      241 ttgagaaatt tgacagatat caagttatgg ggtatttggc tattattact acttatcgct
      301 tttgctagtg attggttagc ctatgtcttt ttcaaaaaaa tgattcgctt gaggcgggaa
      361 ctagggaaat ctaaaggcgg aaggcattga tttatactct tcgaaaatct cttcaaacca
      421 cgtcaacgtc gccttggatt atatatgtaa ctgacttcgt cagtcttatc tacaacctca
      481 aagcagtgct ttgagcaact tgcggctagt ttcctagttt gctctttgat tttcattgag
      541 tattatatta ctttctattt gtaggaggtg gcttatgaag attcctctct taactttggc
      601 aaggcataaa tttgtttatg tcttacttac tttgcttttt cttgctttgg tttatcgtga
      661 tgttttgatg acttatttct tttttgatat tcatgcgccc gatctagcta aattcgatgg
      721 acaagcaatt aaaaatgact tattaaaatc agcattagat tttcgtattc tccagttcaa
      781 tctaggtttt tatcaatcat ttattattcc aatcatcatt gttttgctag gttttcaata
      841 tattgagctg aaaaataaag ttttacgatt gagtattgga agagaagtga gttatcaagg
      901 gttaaaaaga aagttgactt tgcaagttgc aagtatccct tgtttgatat atttagtgac
      961 tgtgctgata attgcaatta taacctattt ctttgggact ttttctcctc ttggatggaa
     1021 ttctatattt tctgatggaa gtggtttaca aagactccta gatggagaga taaaaagcta
     1081 tttgttcttt acttgtgtcc tactaatcgg tattttcatc aatgcaatct attttttaca
     1141 aatagttgat tatgtgggga atgtgactcg ttcggcaatc acctatttga tgtttctttg
     1201 gcttggttct atgctgctct atagtgcctt gccttactat atggttccta tgacgagttt
     1261 gatgcaagct agctatgggg atgtaagttt gatgaaactc tttactcctt atatccttta
     1321 tattgtccct tatatggtgc ttgaaaaata tgaagataat gtttaagaat tttaacaata
     1381 tttggctaaa gagaaagatc gttttactac ttcgtatagt tctgatgatg attttgataa
     1441 actatctatt gtcaacagcg gttcaaaagc aggatgctgt tatctttttc aagagagaat
     1501 tgatttcaat cttttcctat aatgactatt ctgaagcgaa tttagaaatc cccaaactct
     1561 tgttaaacct ttcgcttttc atggtaggat ggctctctgt cattttactt gaaagtgatt
     1621 tggcagacca ttaccatcac ttgattcgct atcaatcaag ctcctttttc gattatacaa
     1681 ggaaacgatt ggttgtcatt tctaaatttt ttactcaaga tttacttgtc tggttccttg
     1741 gtttacttcc gctaggaatt catttcaaaa cagtcgcact tttcttttta cttgctcagt
     1801 taatgatgtt gtacttacta ctgtcttatc tgatagcact gattagtgcg ggcgctggtt
     1861 tttccttttt tctctatttt ttagcatttg tgggacaaga atggatgatg gatcatattg
     1921 taacagtgta tttactactc ttaagcttat tagttatgtt gattgttagt cgcttggaag
     1981 agaaatttaa gaaaggataa acgatgaaac ttgaaattat aaatggacag aaaatttatg
     2041 ggaaaagacc tattttaaat cagttgaatt tggtgtttca atcaggaaaa atttatggac
     2101 ttaaaggtga taatggatct ggcaagacgg ttcttttaaa ggtacttgct ggttatatta
     2161 agcttgacaa aggaaaagtt cttcaagatg gtaaagttta cgggataaaa aatcattata
     2221 ttcaggatgc aggaatttta attgaaaaag tcgagttttt atctcattta tccctgagag
     2281 aaaatttgga actgttaagg tatttttcat ctaaagttac ggaaaaaaga attgcctatt
     2341 ggattcaata ctatgattta caggaatttg aagacgttga ataccggcat ttatccttgg
     2401 gaacaaagca aaaaatggcc ttgattcaag cctttatttc ctctccttct atactctttc
     2461 tcgatgaacc tatgaatgct ttggatgaga agagtgtgag gttaaccaaa caggtcattt
     2521 tatcttacct gaaaaaagaa aatggtctgg ttatcctgac gtcgcacata tcggaagata
     2581 tttcagacct ttgtacagat gtattagttg tcgaaaatgg acatatacaa atgtaaagga
     2641 tatacaatcc taggagatgg cttatggcac atctaaaatc atttattaca cgctattcca
     2701 aggtttatat tggtttagtt ctgctgatct ggctgtcttt cttctttatc ccttgggata
     2761 gagcgattct gggggtaagg attgacatct tcatcataca gaaaatcttg ctggtttttg
     2821 gtattctgtc cattttgatg gccttcttat ccaagaaagt cagtctcttc gtttttggac
     2881 tgatttgctg tctttctctt tggataaatc tatttatcct atttgctctt ttgccgattt
     2941 ttggcaatta aagcatcata aaagtcagag aggttaactt ggaaactagc ctctttttcc
     3001 ttttcaaaat ggggattctt ccttgaaaat aatcagtaat tgtgctaaaa ttaaaagaac
     3061 attctaaaat attcggaatt taaagtaagg aaaaacatgg ctaatatttt aaaaacaatt
     3121 atcgaaaatg ataaaggaga aatccgtcgt ctggaaaaga tggctgacaa ggttttcaaa
     3181 tacgaagacc aaatggctgc tttgactgac gaccaactaa aagcaaaaac agttgaattt
     3241 aaggaacgtt atcaaaatgg agaatcactg gattcattgc tttacgaagc atttgcggtt
     3301 gtccgtgaag gtgccaaacg tgtcctaggt ctcttccctt ataaggttca ggtcatgggg
     3361 gggattgttc ttcaccatgg tgacgtgcca gagatgcgta caggggaagg gaaaaccttg
     3421 actgcgacca tgccggtata cctcaatgcc ctttcaggta aaggggttca cgtagttacg
     3481 gttaatgaat acttgtcaga acgtgacgcg actgagatgg gtgaattgta ctcttggctt
     3541 ggtttgtcag tagggattaa cttggctacc aaatctccaa tggagaaaaa agaagcctat
     3601 gagtgtgata ttacttactc aactaactca gaaatcggat ttgactacct tcgtgacaat
     3661 atggttgttc gcgctgaaaa catggtacaa cgtccgctta actatgcctt ggtcgatgag
     3721 gttgactcta tcttgattga cgaggctcgt acacctttga ttgtatcagg tgccaatgcg
     3781 gttgaaacca gtcagttgta tcacatggca gaccactatg taaaatcttt gaacaaagat
     3841 gactacatca tcgatgtgca gtctaagact attggtttgt ctgattcagg gattgacagg
     3901 gctgaaagct acttcaaact tgaaaacctc tatgacatcg aaaacgtggc tttgacccac
     3961 tttatcgata acgcccttcg tgccaactac atcatgcttc tcgatattga ctatgtggtg
     4021 agcgaagagc aagaaatctt gattgtcgac caatttacag gtcgtaccat ggaaggtcgt
     4081 cgttattctg atggattgca ccaagctatt gaagccaaag aaggtgtgcc aatccaggat
     4141 gaaaccaaga catctgcctc aatcacgtac caaaaccttt tccgtatgta caaaaaattg
     4201 tctggtatga cgggtacagg taagactgag gaagaagaat ttcgtgaaat ctacaacatt
     4261 cgtgttattc caatcccaac aaaccgtcct gttcaacgta ttgaccactc agaccttctt
     4321 tatgcaagta tcgaatctaa gtttaaagcg gttgtcgaag acgttaaggc tcgttaccaa
     4381 aagggtcaac ctgtcttggt tggtacagta gcggttgaaa ctagtgacta catttctaag
     4441 aaattggttg cagctggtgt tcctcacgaa gtcttgaatg ccaaaaacca ctatagagaa
     4501 gcccaaatca tcatgaatgc tggtcaacgt ggtgccgtta ccatcgcaac caacatggcg
     4561 ggtcgtggta ccgacatcaa gcttggtgaa ggtgttcgtg aacttggagg actttgtgtt
     4621 attggtacag aacgtcatga aagtcgtcgt atcgataacc agcttcgtgg acgttcaggt
     4681 cgtcaaggag atccaggtga gtcacaattc tacctatctc ttgaagatga tttgatgaaa
     4741 cgttttggtt ctgaacgctt gaagggaatc tttgaacgtc tcaacatgtc tgaagaggcg
     4801 attgagtctc gcatgttgac gcgtcaggtt gaagcagcgc aaaaacgtgt cgaaggaaat
     4861 aactacgata cccgtaaaca agtccttcaa tacgacgatg tcatgcgtga acaacgtgag
     4921 attatctata ctcaacgtta cgatgtcatc actgcagatc gtgacttggc acctgaaatt
     4981 cagtctatga tcaaacgcac gattgaacgt gtcgttgatg gtcatgcgcg tgccaaacaa
     5041 gatgaaaaac tagaggcaat tttgaacttt gctaagtaca acttgcttcc tgaagattct
     5101 attacgatgg aagacttgtc aggcttgtct gataaggcca tcaaggaaga gcttttccaa
     5161 cgtgccttga aggtttacga tagtcaggtt tcaaaactac gcgatgaaga agcagttaaa
     5221 gaattccaaa aagttttgat tctacgagtg gtggataaca agtggacaga tcatatcgat
     5281 gcccttgatc aattgcgtaa cgcggttgga cttcgtggct atgctcagaa caaccctgtt
     5341 gttgagtatc aggcagaagg tttccgtatg tttaatgata tgattggttc gattgagttt
     5401 gatgtgacac gcttgatgat gaaagcacaa attcatgaac aagaaagacc acaggcagaa
     5461 cgtcatatca gtacaacagc gactcgcaat atcgctgctc accaagcaag tatgccagaa
     5521 gatttggatt tgagccagat tggacgcaat gaactttgcc catgtggttc tggtaagaaa
     5581 tttaaaaact gtcacggtaa aagacaataa aatgagatag tttagaggcg gatatcttgt
     5641 gaaaagtaaa tttttactgg gtatccgttt gctttataag gagatgagtt atggtatttg
     5701 cagcaaaaag ctctaaaata aatatagaag aagttcgtgc cttgtcaaaa ttagaaggtc
     5761 aggctttgga gaggaaatca cagcgagatc aagagctaga agccattata cgtggagaag
     5821 accagcgaat tctcttggta atcgggccat gctcatctga caacgaagaa gctgtccttg
     5881 aatacgctaa gcgtttggca gtcctacaag aagaagtggc agatcgtatc tttatggtta
     5941 tgcgtgttta tactgccaaa ccccgtacca acggagatgg ctataagggc ttgattcacc
     6001 agcctaacgc gacagaagcg cctagtctta tcaatggaat caaagccgtt cgccatcttc
     6061 actatcgtgt catcacagaa acagggatga caactgctga tgaaatgctt tatcctgaaa
     6121 accttccgct tgtagatgat ttgatttctt acatggcagt tggtgcccgt tcagttgaag
     6181 accagcaaca ccgctttgtg gcaagtgggg caggattttc tactggtttt aaaaatccaa
     6241 cctctggaaa tctcaatgtc atgtttaatg ggatttatgc tgctcaaaac aaacaaagtt
     6301 tccttttctt aggaaaagaa gtagaaacaa ctgggaaccc gctttcacac gctattcttc
     6361 gtggtgctct taatgagtat ggaaaaaata ttcccaacta ctattatgac aatttaattg
     6421 ataccattgc ccagtatgag aaaatgggct tggaaaatcc ttttatcatc attgatacca
     6481 atcatgacaa ttctggtaag cagtatattg aacagatccg aattgtccgc cagaccttga
     6541 ttaaccgtgc ttggaatgaa aaaattaagc agttcgttcg tggttttatg attgagtctt
     6601 atctggaaga tggtcgacaa aatgagccag aagtatttgg taagtctatc acagaccctt
     6661 gcctgggttg ggataacaca gaagctcttg tcagagaaat ttacaaaacg ttaggagaat
     6721 aagatggcat ttattgaaaa aggtcaagaa atcgatatgg aagtcatcaa ggctgaaacc
     6781 caattgtctg cggaagcctt gagactcaag gaaagccgtg acagggaatt ggcagatatt
     6841 atttcagggg aagatgaccg tattctcttg gtgattggtc cttgctcttc tgataatgaa
     6901 gaggcggtct tggaatatgc tcgccgttta tccgccttgc aaaagaaggt agcggacaag
     6961 attttcatgg tcatgcgcgt gtatactgct aagcctcgta ccaatggaga cggctataaa
     7021 ggattggttc accagccaga tacttctaag gctccaagcc tgattaacgg cttgcaggct
     7081 gtgcgccagt tgcactaccg cgtgattaca gagactggtt tgacaacggc agatgagatg
     7141 ctttatccgt caaatctgat cttggtggat gacttggtca gctaccatgc cgttggagct
     7201 cgttctgtgg aagaccaaga gcaccgcttt gtggcttctg ggattgatgc accagtaggg
     7261 atgaaaaatc caacctcagg aaatttgggt gttatgttta acgccatcta tgctgctcaa
     7321 aacaagcaaa ccttccttta tcatgggcag gaagttgaga catcaggtaa tcctttggcc
     7381 catgttatcc tccgtggagc agtcaacgag tatggcaatt atatgccgaa ttactactat
     7441 gaaaacctac tccaagccat tgaacgctat gaaaccatgg gacttgaaaa tccttttatc
     7501 ctcattgaca ccaaccatga taactcaggc aagcaatata tggagcagat tcgaattgtt
     7561 cgccagacct tgcagaatcg tgattggaat gagaaaatta aaaagacggt tcgaggattt
     7621 atgattgaat cttacctagc agatggtcgt caaaaccaac cagaaatctt tggttgctct
     7681 attactgacc cttgcctagg ttgggaaaat acagaggcct tggtagaaga aatctatgct
     7741 accttgacaa aataagtgaa aaggatggag ttggggaatc tcaactcctt ttgatgagaa
     7801 tgatagttgg acacggaatt gacatcgaag aattggcttc gatagaaagc gcagttacac
     7861 gacatgaagg atttgctaag cgtgtactga ccgctcagga aatggagcgc ttcaccagtc
     7921 tcaaaggacg caggcaaata gaatatttag ctggtcgctg gtcggctaag gaggcctttt
     7981 ccaaggctat gggaacgggc attagcaagc tcggttttca ggatttggaa gtcttgaaca
     8041 atgaacgtgg ggcgccttat tttagtcagg caccattttc aggaaagatt tggctgtcta
     8101 tcagccacac cgatcagttt gtgacagcca gtgtcatttt ggaggaaaat catgaaagct
     8161 agtccacata gaccaaccaa ggctctgatt catctgggag ctattcgaca aaatattcag
     8221 caaatggggg ctcatatccc tcaaggaacg ctcaagtggg ctgtggtcaa ggccaatgct
     8281 tatggtcatg gagctgttgc cgttgccaag gcaattcaag atgatgttga tggcttttgc
     8341 gtttccaata tcgatgaagc cattgaactc agacaagctg gactcagcaa gccaatcctc
     8401 attttaggag tttctgaaat cgaagctgtt gctctagcta aagaatatga cttcaccttg
     8461 acagtggctg gactggagtg gattcaagca ctcttagata aggaagtgga cctaactgga
     8521 ttgacagtcc acctcaagat tgattcaggg atgggacgga ttggttttag agaggtcagt
     8581 gaggttgagc aggctcaaga tttgctccaa aaacacggtg tttgtgttga aggaatcttt
     8641 acccactttg ctactgctga tgaggaatca gatgactatt ttaatgccca gttagaacgg
     8701 tttaaaacta ttttagctag tatgaaggaa gttccagagc tggttcatgc tagcaattct
     8761 gcaacgactc tttggcatgt agagactatt ttcaatgcgg ttcgtatggg agatgccatg
     8821 tatggcctca atccaagtgg agcggtcttg gatttgcctt atgatttgat accggccttg
     8881 accttggagt ctgctctggt tcatgtcaag acagttccag ctggagcttg catgggctat
     8941 ggagcaactt atcaagcgga tagcgagcaa gtcatcgcga ccgtgccaat cgggtatgca
     9001 gatggatgga caagagacat gcaaaatttc tctgtcttgg tagatggcca agcttgccca
     9061 attgtcggca gggtttcgat ggaccaaatc actattcgat tgcctaagct ttatccgcta
     9121 ggaaccaagg taaccttgat tggctccaat ggggataagg aaatcactgc aactcaggta
     9181 gcgacctacc gcgtcaccat taactatgag gtggtttgcc tcctcagcga ccgtattccg
     9241 agagaatatt attagaaaag aaaggagtgg agcatgaatc tacatcaacc cttgcatgtc
     9301 ttgcctggtg tgggaccaaa gtcagcagaa aaatacgcca aactaggaat tgaaaacttg
     9361 caagatctct tgctctactt tcctttccgt tatgaagact tcaaaaccaa gcaggtgctg
     9421 gaactagaag acggtgagaa ggcagttctt tctggtcagg tagtgactcc tgctagtgtc
     9481 cagtattatg gtttcaagcg caatcgcctg cgttttagtc tcaagcaggg agaggtcgtt
     9541 tttgcggtga atttctttaa ccagccctat ctggctgata aaatagagtt gggagcaacc
     9601 cttgctgtct ttggaaaatg ggaccgcgct aaggctagtc tgactgggat gaaggttctg
     9661 gctcaggtag aagatgacct ccagcctgtc tatcgtctgg ctcagggaat cagtcaggcc
     9721 agtctggtta aggtcatcaa gacggctttt gatcagggac tggacctctt gatagaagaa
     9781 aatctgcccc agtctttact agacaaatac aaactcatgt cccgttgtca ggcagtccga
     9841 gctatgcatt ttccaaagga tttggcagaa tacaagcagg ctcttcgccg tatcaagttt
     9901 gaggaactct tttatttcca aatgcagttg cagacgctca agtctgaaaa tagagttcag
     9961 ggaagtggtc tggttctgaa ttggtctcag gaaaaagtga cagcagttaa agtaagtctt
    10021 ccttttgccc tgacccaagc tcaggaaaag agtttgcagg aaattttaac tgatatgaag
    10081 tccgaccacc acatgaatcg tctcctacaa ggggatgtgg ggagtggaaa aacggtagtc
    10141 gctggcttgg ccatgtttgc ggcagtgaca gcaggttatc aggctgccct aatggtacca
    10201 acagaaatcc tcgcagagca acactttgag agtttacaga acctttttcc caatttgaaa
    10261 ctggctctct tgacaggttc cttgaaagct gcagaaaaga gagaggtctt ggagaccatt
    10321 gccaagggtg aggctgattt gattatcgga actcacgctc tgatacaaga tggggtggag
    10381 tatgctcgtc ttggtttgat tattatcgat gagcagcacc gttttggtgt agggcaaagg
    10441 cgtattttac gggaaaaagg tgacaatcca gatgtcctca tgatgacggc gactcccatt
    10501 ccacggacgc ttgccatcac agcctttgga gatatggatg tttccattat cgaccagatg
    10561 ccagcaggtc ggaagcctat tgtgacgcgc tggatcaaac atgagcaact acctcaggtc
    10621 ttgacttggt tagaggggga aattcaaaaa ggttcccaag cctatgtcat ctctcctttg
    10681 attgaagaat cagaagctct agatttgaaa aatgccattg ccttatcaga ggagttgacg
    10741 actcattttg caggcaaggc agaggtggct cttctacatg gtaggatgaa gagtgacgaa
    10801 aaagaccaga tcatgcagga tttcaaggag agaaagacgg atattctggt ttcgacgacg
    10861 gttattgagg ttggggtcaa cgttcccaat gcgactgtca tgattatcat ggatgccgat
    10921 cgcttcggtc tcagtcaact tcaccagctt agaggtcgtg tcggtcgggg ggacaagcag
    10981 tcctacgctg ttctcgttgc caatcccaag acggattctg ggaaagaccg catgcgcatc
    11041 atgacagaaa cgaccaatgg atttgtcctt gcggaggaag atttgaaaat gcgtggttct
    11101 ggtgagattt ttggaaccag acagtcagga cttccagagt tccaagtggc tgatattatc
    11161 gaagattttc cgattttaga agaagcaaga aaggttgcta gctacattag ttctatagaa
    11221 gcttggcaag aagatccaga gtggcgcatg attgcccttc atctggaaaa gaaagaacat
    11281 ctggattaag ctttctctaa ggaaaactta agctagtttt taggtttggc tcttatacta
    11341 aagtcatcaa aaagaaacga ggactctcat atgacagtaa cgattaaagt aaattaccaa
    11401 accactttcc aaaagaagga agcaaaaaac tagtataaac agaagagaga gcgaaatgct
    11461 ctttttcgtt tctaaaacta ctttcagccc atcatcctaa aagtaaagaa tctaaattca
    11521 ctttctattt atccttcttt cttgcattga ttacatagat atgctacagt tgtggtaacg
    11581 attacaaaat aaaaggagca tgctatgaaa aatccagctt tgctagaaga aattaagacc
    11641 tatagaggaa gggatgaggt tccggaagac tttgatgctt tctgggatgg ggaagtgaaa
    11701 aatgtttcca cacctccatc ctaccacttg gaggaaagag atttccacat tcctcaagtc
    11761 aagtgctatg agttaacatt tgaaggaagc aaggaaggaa aggtctatgc acgcattgtt
    11821 cttccaaaga gtgaggagaa ggttccacta atcttccatt ttcatggtta tatgggacgt
    11881 ggctgggact gggccgacat gctgggcttc accgtagctg gttacggtgt tgtttccatg
    11941 gatgtgcggg gccagtcagg ttactcacaa gacggcttgc gttctccttt aggaaatacc
    12001 gtgaaggggc atattatccg tggtgctatg gaaggtcggg accacctctt ttataaggat
    12061 gtttatctgg atatttacca gttggtcgaa attgttgcta gtctgtctca ggttgatgag
    12121 aagcgtcttt ctagctatgg tgcctcacaa ggaggggctc tagctctagt tgcagcagcg
    12181 ctcaatcctc gaattcagaa aacagttgcc atttatccct tcttgtcaga cttcagacgg
    12241 gtgattgaga ttggtaatac tagcgaggct tacgacgaac ttttccgtta tttcaagttt
    12301 cacgacccct tccatgaaac agaggaggaa atcatggcga cccttgccta tatcgatgtc
    12361 aaaaatcttg cccatcgtat ccaaggtgag gttaagatga ttacgggctt ggacgacgat
    12421 gtttgctatc ccattaccca gtttgcgatt tataatcgtc tgacctgcga taaaacctat
    12481 cgtatcatgc ctgagtatgc tcacgaagcc atgaatgtat ttgtcaatga ccaagtctac
    12541 aactggctct gtggaagtga gattcctttt aaatatctaa aataaggagt cgactctaag
    12601 cacaaaatct taaaaattac aaacacgcat agtatcaggg gattaaaaaa acttgatact
    12661 atgcgtttta tcatggaaat atagtaaaat gaaacaagaa
//
LOCUS       DAVKZB010000051        12178 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKZB010000051 DAVKZB010000000
VERSION     DAVKZB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.051"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..210
                     /locus_tag="VXA80_001250"
     CDS             <1..210
                     /locus_tag="VXA80_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353746.1"
                     /translation="TKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLPVHGF
                     CPRGTYDLGYIGGGNHLCKGSAARF"
     gene            264..935
                     /locus_tag="VXA80_001251"
     CDS             264..935
                     /locus_tag="VXA80_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353747.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(987..1184)
                     /locus_tag="VXA80_001252"
     CDS             complement(987..1184)
                     /locus_tag="VXA80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0353748.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1177..2085
                     /locus_tag="VXA80_001253"
     CDS             1177..2085
                     /locus_tag="VXA80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0353749.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2082..2543
                     /locus_tag="VXA80_001254"
     CDS             2082..2543
                     /locus_tag="VXA80_001254"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV0353750.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2533..3420
                     /locus_tag="VXA80_001255"
     CDS             2533..3420
                     /locus_tag="VXA80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0353751.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3423..5306
                     /gene="cbpE"
                     /locus_tag="VXA80_001256"
                     /gene_synonym="pce"
     CDS             3423..5306
                     /gene="cbpE"
                     /locus_tag="VXA80_001256"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV0353752.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELSVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5386..6516
                     /locus_tag="VXA80_001257"
     CDS             5386..6516
                     /locus_tag="VXA80_001257"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV0353753.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6526..7788
                     /locus_tag="VXA80_001258"
     CDS             6526..7788
                     /locus_tag="VXA80_001258"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV0353754.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7792..8589
                     /gene="proC"
                     /locus_tag="VXA80_001259"
     CDS             7792..8589
                     /gene="proC"
                     /locus_tag="VXA80_001259"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV0353755.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTSASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            8705..9343
                     /locus_tag="VXA80_001260"
     CDS             8705..9343
                     /locus_tag="VXA80_001260"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV0353756.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9340..10230
                     /locus_tag="VXA80_001261"
     CDS             9340..10230
                     /locus_tag="VXA80_001261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV0353757.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10270..10587
                     /gene="yabA"
                     /locus_tag="VXA80_001262"
     CDS             10270..10587
                     /gene="yabA"
                     /locus_tag="VXA80_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV0353758.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10590..11459
                     /gene="rsmI"
                     /locus_tag="VXA80_001263"
     CDS             10590..11459
                     /gene="rsmI"
                     /locus_tag="VXA80_001263"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV0353759.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11686..>12178
                     /locus_tag="VXA80_001264"
     CDS             11686..>12178
                     /locus_tag="VXA80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV0353760.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKI"
BASE COUNT         3652 a         2084 c         2907 g         3535 t
ORIGIN      
        1 acaaaaaata taaatctaac aaatgaagaa ttagagctga tacaaggtgg agcagattca
       61 tatggtaaaa atcctaatgg taggtacgat tgggaaatag aaccagtatt aactctgccg
      121 gttcatggat tttgtcccag aggcacctat gatttaggat atattggagg aggcaatcat
      181 ctttgcaaag gaagtgctgc gagattttaa gtaaaattta ttagtaatat gaagaaacaa
      241 ggggagaaag cagagaattt aatatgaaaa aacgggctat tcaaatttta ctagcattgt
      301 ccttaatttt ttacaaatca acttggtttt ggagagtttt caatcacctc gcaaagccct
      361 atctaccagc aagtcgtgaa ttttttcaga ttctgctttt gatggagagc ggagttcttt
      421 tcttagcggt catctatcta ctggtttttg caggaaagaa aatttttcat ttcaagtggc
      481 agctgaggta cttcatctac cttttactgg gctacatcat ttcatatatg tctgacttcc
      541 tcttttcgta tttcatatcc ctgtcttcaa atcagatttc tttgaatgaa acgatagaaa
      601 tgatggggag acaggagttc ccttatttct tgctcatcgt ttgcttcatc gcccctattg
      661 ctgaggaatt gatttatcga ggtgtgctta tgacaacctt tttcaaaaac tcaccttggt
      721 acggagatgt tttgctttct gctattattt tcggttatat tcatatcaat tttgctttaa
      781 ctcctcttgc ttttttcatt tatgctagtg gaggtctgat tttagctcta ttgtatcgca
      841 tgactaaaaa tctctactat ccaatactag ttcatattct catcaatatc actgccttct
      901 gggatgtttg gttactccta ttttcaggaa gttagcttac taaaaaaatg tcggaatttt
      961 ccggcatttt cttttttcac aaatagtcaa cgtttttctt tccgatactg aagtggtgtg
     1021 tagccactta tttttttgaa ttgattttga aaataagatt ggcgtgagaa aggcagatag
     1081 tgaagatagt taagaagaat aggatgttct tttttccttt ttggaaaact tctaaaatat
     1141 ggtataatga aaagataaag aagttggggg tagaagatga acattcaaca attacgctat
     1201 gttgtggcta ttgccaatag tggtactttt cgtgaagctg ctgaaaagat gtatgttagt
     1261 cagccgagtc tgtctatttc tgttcgtgat ttggaaaaag agttgggctt taagattttc
     1321 cgtcggacca gctcagggac tttcttgacc cgtcgtggga tggaatttta tgaaaaagcg
     1381 caagaattgg ttaaaggatt tgatattttt caaaatcagt atgccaatcc tgaagaagaa
     1441 aaagatgaat tttccgttgc tagccagcac tatgacttct taccaccaac tattacggcc
     1501 ttttcagagc gctatcctga ctataagaac ttccgtattt ttgaatcaac tactgttcaa
     1561 atattagatg aagtggcgca agggcatagt gagattggga ttatctacct caacaatcaa
     1621 aataaaaagg ggattatgca acgggttgaa aagttaggtc tggaggtcat cgaattgatt
     1681 cctttccata cccatattta tctctgtgag ggtcatcctt tagcccagaa agaggaatta
     1741 gtcatggagg atttagcgga tttaccaacg gttcgtttca ctcaagagaa agacgagtac
     1801 ctttattatt cagagaactt tgtcgatacc agcgctagct cacagatgtt taatgtgaca
     1861 gaccgtgcca ccttgaatgg tattttggag cggacggacg cctatgcgac aggttctgga
     1921 tttttagata gtgacagtgt taatggcatt acagttattc gtctcaagga taacctagat
     1981 aaccgcatgg tctatgttaa acgtgaagaa gtggagctta gtcaagctgg gactctcttc
     2041 gtagaagtca tgcaagaata ttttgatcaa aagaggaaat catgaaaaaa agagcaatag
     2101 tggcagtcat tgtactgctt ttaattgggc tggatcagtt ggtcaaatcc tatatcgtcc
     2161 agcagattcc actgggtgaa gtgcgctcct ggattcccaa tttcgttagc ttgacctacc
     2221 tgcaaaatcg aggtgcagcc ttttctatct tacaagatca gcagctgtta ttcgctgtca
     2281 ttactctggt tgtcgtgata ggtgccattt ggtatttaca taaacacatg gaggactcat
     2341 tctggatggt cttgggtttg actctaataa tcgcgggtgg tcttggaaac tttattgaca
     2401 gggtcagtca gggctttgtt gtggatatgt tccaccttga ctttatcaac tttgcaattt
     2461 tcaatgtggc agatagctat ctgacggttg gagtgattat tttattgatt gcaatgctaa
     2521 aagaggaaat aaatggaaat taaaattgaa actggtggtc tgcgtttgga taaggctttg
     2581 tcagatttgt cagaattatc acgtagtctc gcgaatgaac aaattaaatc aggccaggtc
     2641 ttggtcaatg gtcaagtcaa gaaagctaaa tacacagtcc aagagggtga tgtcgtcact
     2701 taccatgtgc cagaaccaga ggtattagag tatgtggctg aggatcttcc gctagaaata
     2761 gtctaccaag atgaggatgt ggctgtcgtt aacaaacctc agggaatggt tgtgcacccg
     2821 agtgctggtc ataccagtgg aaccctagta aatgccctca tgtatcatat taaggacttg
     2881 tcgggtatca atggggttct gcgtccaggg attgttcacc gtattgataa ggatacgtca
     2941 ggtcttctca tgattgctaa aaacgatgat gcgcatctag tacttgccca agaactcaaa
     3001 gataaaaagt ctctccgcaa atattgggcg attgttcatg gaaatctgcc taatgatcgt
     3061 ggtgtaattg aagcgccgat tggccggagt gaaaaagacc gtaagaaaca ggctgtaact
     3121 gctaaaggga agcctgcagt gacgcgtttt cacgtcttgg aacgctttgg cgattatagc
     3181 ttagtagagt tgcaactgga gacagggcgc actcatcaaa tccgtgtcca catggcttat
     3241 atcggccatc cagtcgctgg tgatgaggtc tatggtcctc gcaagacttt gaaaggacat
     3301 ggacaatttc ttcatgccaa gactttaggt tttactcatc cgagaacagg taagaccttg
     3361 gaatttaaag cagatatccc agagattttt aaggaaacct tggagagatt gagaaagtaa
     3421 gaatgaaaaa gaaattaact agtttagcac ttgtaggcgc ttttttaggt ttgtcatggt
     3481 atgggaatgt tcaggctcaa gaaagttcag gaaataaaat ccactttatc aatgttcaag
     3541 aaggtggcag tgatgcgatt attcttgaaa gcaatggaca ttttgccatg gtggatacag
     3601 gagaagatta tgatttccca gatggaagtg attctcgtta tccatggaga gaaggaattg
     3661 aaacgtctta taagcatgtt ctaacagacc gtgtctttcg tcgtttgaag gaattgagtg
     3721 tccaaaaact tgattttatt ttggtgaccc atacccacag tgatcatatt ggaaatgttg
     3781 atgaattact gtctacctat ccagttgacc gagtctatct taagaaatat agtgatagtc
     3841 gtattactaa ttctgaacgt ctatgggata atctgtatgg ctatgataag gttttacaga
     3901 ctgctgcaga aaaaggtgtt tcagttattc aaaatatcac acaaggggat gctcattttc
     3961 agtttgggga catggatatt cagctctata attatgaaaa tgaaactgat tcatcgggtg
     4021 aattaaagaa aatttgggat gacaattcca attccttgat tagcgtggtg aaagtcaatg
     4081 gcaagaaaat ttaccttggg ggcgatttag ataatgttca tggagcagaa gacaagtatg
     4141 gtcctctcat tggaaaagtt gatttgatga agtttaatca tcaccatgat accaacaaat
     4201 caaataccaa ggatttcatt aaaaatttga gtccgagttt gattgttcaa acttcggata
     4261 gtctaccttg gaaaaatggt gttgatagtg agtatgttaa ttggctcaaa gaacgaggaa
     4321 ttgagagaat caacgcagcc agcaaagact atgatgcaac agtttttgat attcgaaaag
     4381 acggttttgt caatatttca acatcctaca agccgattcc aagttttcaa gctggttggc
     4441 ataagagtgc atatgggaac tggtggtatc aagcgcctga ttctacagga gagtatgctg
     4501 tcggttggaa tgaaatcgaa ggtgaatggt attactttaa ccaaacgggt atcttgttac
     4561 agaatcaatg gaaaaaatgg aacaatcatt ggttctattt gacagactct ggtgcttctg
     4621 ctaaaaattg gaagaaaatt gatggaatct ggtattattt taacaaagaa aatcagatgg
     4681 aaattggttg ggttcaagat aaagagcagt ggtattattt ggatgttgat ggttctatga
     4741 agacaggatg gcttcaatat atggggcaat ggtattactt tgctccatca ggggaaatga
     4801 aaatgggctg ggtaaaagat aaagaaacct ggtactatat ggattctact ggtgtcatga
     4861 agacaggtga gatagaagtt gctggtcaac attattatct ggaagattca ggagctatga
     4921 agcaaggctg gcataaaaag gcaaatgatt ggtatttcta caagacagac ggttcacgag
     4981 ctgtgggttg gatcaaggac aaggataaat ggtacttctt gaaagaaaat ggtcaattac
     5041 ttgtgaacgg taagacacca gaaggttata ctgtggattc aagtggtgcc tggttagtgg
     5101 atgtttcgat cgagaaatct gctacaatta aaactacaag tcattcagaa ataaaagaat
     5161 ccaaagaagt agtgaaaaag gatcttgaaa ataaagaaac gagtcaacat gaaagtgtta
     5221 caaatttttc aactagtcaa gatttgacat cctcaacttc acaaagctct gaaacgagtg
     5281 taaacaaatc ggaatcagaa cagtagtaga aaagaaggtt ttagggcctt ctttttccta
     5341 tcaactcttt tctatttcct gttattcatg ttataatgga taaatatgaa taatcggagt
     5401 gagactatga aatacaaacg gattgtcttt aaggtgggta cttcttctct gacaaatgag
     5461 gatggaagtt tatcacgtag taaggtaaag gatattaccc agcagttggc tatgctgcac
     5521 gaggctggtc atgagttgat tttggtgtct tcaggtgcca ttgcggctgg ttttggagcc
     5581 ttaggattta aaaagcgtcc gactaaaatt gctgataaac aggcttcagc agcggtaggg
     5641 cagggacttt tgttggaaga atatacgacc aatcttctct tgcgtcaaat cgtttctgca
     5701 caaatcttgc tgacccaaga tgactttgtg gataagcgtc gttataaaaa tgcccatcag
     5761 gctttgtcgg ttttgctcaa ccgtggggca attcctatca tcaatgagaa tgatagtgtc
     5821 gttattgatg agctcaaggt tggggacaat gacactctaa gtgctcaagt agcggcgatg
     5881 gtacaagcag accttttagt tttcttgaca gatgtggacg gtctctatac tggaaatcct
     5941 aattcagatc caagagccaa acgcttggag agaatcgaga ccatcaatcg tgagattatt
     6001 gatatggctg gtggagctgg ttcgtcaaac ggaactgggg gtatgttaac caaaatcaag
     6061 gctgcaacta tcgcgacgga atcaggagtt cctgtttata tctgctcatc cttgaaatca
     6121 gattccatga ttgaggcggc agaggagacc gaggatggtt cttactttgt tgctcaagag
     6181 aaggggcttc gtacccagaa acaatggctt gccttctatg ctcagagtca aggttctatt
     6241 tgggttgata aaggggctgc ggaagctctc tctcaacatg gaaagagtct tctcttatct
     6301 ggtatcgttg aagcagaagg agtcttttct tacggtgata tcgtgacagt atttgataag
     6361 gaaagtggaa aatcacttgg aaaaggacgc gtgcaatttg gagcatctgc tttggaggat
     6421 atattgcgtt ctcaaaaagc caagggtgtc ttgatttacc gtgacgactg gatttccatt
     6481 actcctgaaa tccaactact ttttacagaa ttttagaggt aaactatggt gagtagacaa
     6541 gaacaatttg aacaggtaca ggctgttaaa aaatcgatta acacagctag tgaagaagtg
     6601 aaaaaccaag ccttgctagc catggctgat cacttagtgg ctgctactga ggaaatttta
     6661 gcggctaatg ccctcgatat ggcagcggct aaggggaaaa tctcagatgt gatgttggat
     6721 cgtctttatt tggatgcaga tcgtatagaa gcgatggcaa gaggaattcg tgaagtggtt
     6781 gccttaccag atccaatcgg tgaagtttta gaaacaagtc agcttgaaaa tggtttggtt
     6841 atcacaaaaa aacgtgtagc tatgggtgtc atcggtatta tctatgaaag ccgtccaaat
     6901 gtgacgtctg atgcggctgc tttgactctt aagagtggaa atgcggttgt tcttcgtagt
     6961 ggtaaggatg cctatcaaac aacccatgcc attgtcacag ccttgaagaa gggcttggag
     7021 acgactacta ttcatccaaa tgtgattcaa ctggtggagg atactagccg tgaaagtagt
     7081 tatgctatga tgaaggccaa gggctatcta gaccttctca ttcctcgtgg aggagctggc
     7141 ttgatcaatg cagtagttga gaatgccatt gtacctgtta tcgagacagg gactgggatt
     7201 gtccatgtct atgtggataa ggatgcagac gaagacaagg cgctgtctat catcaacaat
     7261 gctaaaacca gtcgtccttc tgtttgtaat gccatggagg ttctgctggt tcatgaaaac
     7321 aaggcagcaa gcatccttcc tcgcttggat caaatgctgg ttgcagagcg taaggaagct
     7381 ggactggaac caattcaatt ccgcttggat agcaaagcaa gccagtttgt ttcaggtcaa
     7441 gcagctgaga cccaagactt tgacaccgag tttttagact atgtccttgc tgttaaggtt
     7501 gtgagcagtt tagaagaagc ggttgcgcac attgaatccc acagcaccca tcattcggat
     7561 gctattgtga cggaaaatgc tgaagctgca gcatacttta cagatcaagt ggactctgca
     7621 gcggtgtatg ttaatgcctc aactcgtttc acagatggag gacaatttgg tcttggttgt
     7681 gaaatgggga tttctactca gaaattgcac gcgcgtggtc caatgggctt gaaagagttg
     7741 accagctaca agtatgtggt tgctggtgat gggcagataa gggagtaaga gatgaagatt
     7801 ggatttatcg gtttggggaa tatgggtgct agcttggcaa aatctgtctt gcagactagg
     7861 acgtcagatg agattctcct tgccaatcgt agtcaagcta aggtagatgc tttcattgca
     7921 gactttggtg gtcaggcttc cagcaatgaa gaaatgtttg cagaagcaga tgtgattttt
     7981 ctaggagtta agcctgctca gttttctgaa ctgctttctc aataccagac catccttgaa
     8041 aaaagagaaa gtcttctttt gatttcgatg gcagctggat tgaccttaga aaaactagca
     8101 agtcttatcc caagtcaaca ccgaattatt cgtatgatgc ctaatacctc tgcttctatc
     8161 gggcaaggag tgattagtta tgccttgtct cctaattgca gggctgagga cagtgagctc
     8221 ttttgtcagc ttttatccaa ggctggtctc ttggttgaat taggagaaag cttaatcaat
     8281 gcagcgacag gtcttgcagg ctgtggacca gcctttgtct atctctttat tgaggctttg
     8341 gcagatgcag gtgttcagac gggattacca cgagaaacag ccttgaaaat ggcagcccaa
     8401 actgtggtag gagctgggca attggtccta gaaagccagc aacatcctgg agttttgaaa
     8461 gatcaagttt gtagtccagg cggttcgact attgctggcg tagcaagctt agaagcgcat
     8521 gcttttcgag gcatagtcat ggaggcagtt catcaagcct ataaacgaac acaagaacta
     8581 ggtaaataag aggtagcttt gactgcctct tttatggtgg ctgaaatgag aagacacaaa
     8641 aagattgtca caaactccta tttttttgat agaatagaag tagtaaaaaa gaaatgagtt
     8701 agacatgtca aaaggatttt tagtctctct tgagggacca gagggagcag gcaagaccag
     8761 tgttttagag gctctgctac caattttaga ggaaaaagga gtagaggtgc tgacgacccg
     8821 tgaacctggc ggagtcttga ttggggagaa gattcgggaa gtgattttgg atccaagtca
     8881 tactcagatg gatgcgaaga cagagctttt gctctatatt gccagtcgca gacagcattt
     8941 ggtggaaaaa gttcttccag cccttgaagc tggcaagttg gtcatcatgg atcgttttat
     9001 cgatagttct gttgcctatc agggatttgg tcgtggctta gatattgaag ccattgactg
     9061 gctcaatcag tttgcgacag atggcctcaa acccgatttg acactctatt ttgacatcga
     9121 ggtggaagaa gggctggctc gtattgctgc taatagtgac cgcgaggtta atcgtttgga
     9181 tttggaaggg ttggacttgc ataaaaaagt tcgtcaaggc tacctttctc ttctggataa
     9241 agagggaaat cgcattgtca agattgatgc tagtctccct ttggagcaag ttgtggaaac
     9301 taccaaggct gtcttgtttg acggaatggg cttggccaaa tgaaacaaga tcaactaaag
     9361 gcttggcaac cagctcagtt tgaccgtttt gtccgtatct tagaacaaga ccagctcaat
     9421 cacgcctatc tcttttcagg tttctttgga agcttggaaa tggcgcaatt tttagctaag
     9481 agcctttttt gtacggataa agttggcgtc ttaccatgtg agaaatgccg aagttgcaag
     9541 ctgattgaac aggaagagtt tccagatgtc accttgatta agccagtcaa tcaggtcatc
     9601 aagacagaac gcattcggga attggtggga cagttttctc aagcagggat tgaaagccag
     9661 caacaggtct ttatcatcga gcaagcggat aaaatgcatc ccaacgcagc caattctctg
     9721 ctcaaggtca tcgaagaacc ccagagtgaa gtttatattt tcttcttgac tagcgatgag
     9781 gaaaagatgt taccgacaat ccgaagtcgg actcagatct tccactttaa aaagcaagaa
     9841 gaaaaactta tcttactctt agaacaaatg ggacttgtta agaaaaaagc gactctttta
     9901 gctaagttta gtcaatcgcg agctgaagca gaaaagttgg cacatcaggc aagtttttgg
     9961 accttggtcg atgaaagtga acgcctgctg acttggttag tagctaagaa aaaagaaagt
    10021 tatctacagg ttgccaaatt agccaacttg gcagatgata aggaaaaaca ggatcaggtt
    10081 ttacggattc ttgaagttct ctgtgggcag gacctcttgc aggtaagagt aagagtgatt
    10141 ctacaagatt tactagaagc tagaaaaatg tggcaagcta atgtcagctt tcaaaatgcc
    10201 atggaatatc tggtcttgaa agaaatataa actcaaaaat gaatgataaa gaaaggaaag
    10261 ggctgtttta tggacaaaaa agaattattt gacgcgctgg atgatttttc ccaacagtta
    10321 ttggtaacct tggccgatgt ggaagccatc aagaaaaatc tcaagagcct ggtagaggaa
    10381 aatacagctc ttcgtttgga aaattctaag ttgcgagaac gcttgggtga ggtggaagca
    10441 gatgctcctg tcaaggccaa gcatgttcgt gaaagtgtcc gtcgcattta ccgtgatgga
    10501 tttcacgtat gtaatgattt ttatggacaa cgtcgagagc aggacgagga atgtatgttt
    10561 tgtgacgagt tgctatacag ggagtaggca tgcagattca aaaaagtttt aaggggcagt
    10621 ctccctatgg caagctgtat ctagtggcaa cgccgattgg caatctagat gatatgacct
    10681 ttcgagctat ccagaccttg aaagaagtag attggattgc tgctgaggat acgcgcaata
    10741 caggtctttt gctcaagcat tttgacattt ccaccaagca gatcagtttt catgagcaca
    10801 atgccaagga aaaaattcct gatttgattg gtttcttgaa agcagggcaa agtattgctc
    10861 aggtctctga tgccggtttg cctagcattt cagaccctgg tcatgattta gttaaggcag
    10921 ctattgagga agaaattgca gttgtgacag ttccaggtgc ctctgcagga atttctgcct
    10981 tgattgccag tggtttagcg ccacagccac atatctttta cggtttttta ccgagaaaat
    11041 caggtcagca gaagcaattt tttggcttga aaaaagatta tcctgaaaca cagatttttt
    11101 atgaatcacc tcatcgtgta gcagacacgt tggaaaatat gttagaagtc tacggtgacc
    11161 gctccgttgt cttggtcagg gaattgacca aaatctatga agaataccaa cgaggtacta
    11221 tctctgagtt attagaaagc attgctgaaa cgccactcaa gggcgaatgt cttctcattg
    11281 ttgagggtgc cagtcagggt gtggaggaaa aggacgagga agacttgttc gtagaaattc
    11341 aaacccgcat ccagcaaggt gtgaagaaaa accaagctat caaggaagtc gctaagattt
    11401 accagtggaa taaaagtcag ctctacgctg cctaccacga ctgggaagaa aaacaataaa
    11461 gggagacagg atgtaataat tctgtctgtt tctgtttaac ttaattagtg atgataatat
    11521 aaagatgtat cacttggtat agaagctttg gtattaagtt ttttattaag cccatacgga
    11581 ataccgatgg ttggagcagc agttatagcg ttcttagaag gtataaatag aaaaataagg
    11641 tcattttaaa tcaaaggatt gataaatcag aaagaaggtg attttttgcg aacatacgaa
    11701 aataaagaag aactaaaagc tgagatagag aaaacatttg agaaatatat tttagaattt
    11761 gataatattc cagaaaattt aaaagataag agagctgatg aagttgacag aactccagca
    11821 gaaaaccttg cttatcaggt tggttggacc aacttggttc ttaaatggga agaagatgaa
    11881 agaaaggggc ttcaagtaaa aacaccatcg gataaattta aatggaatca acttggtgaa
    11941 ttatatcagt ggttcacaga tacctacgct catttatctc tgcaagagtt gaaagcaaaa
    12001 ttaaatgaaa atattaattc tatctctgca atgattgatt cgttgagtga ggaagaatta
    12061 tttgaaccgc atatgagaaa gtgggctgat gaagcgacta aaacagcgac ttgggaagtg
    12121 tataagttta ttcatgtaaa tacggttgca ccttttggaa ctttcagaac taaaatca
//
LOCUS       DAVKZB010000052        12169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKZB010000052 DAVKZB010000000
VERSION     DAVKZB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.052"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            70..234
                     /locus_tag="VXA80_001265"
     CDS             70..234
                     /locus_tag="VXA80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0353761.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPAIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            318..884
                     /gene="amaP"
                     /locus_tag="VXA80_001266"
     CDS             318..884
                     /gene="amaP"
                     /locus_tag="VXA80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV0353762.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            896..1066
                     /locus_tag="VXA80_001267"
     CDS             896..1066
                     /locus_tag="VXA80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV0353763.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1105..1713
                     /locus_tag="VXA80_001268"
     CDS             1105..1713
                     /locus_tag="VXA80_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0353764.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1744..1947
                     /locus_tag="VXA80_001269"
     CDS             1744..1947
                     /locus_tag="VXA80_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0353765.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2057..2335)
                     /locus_tag="VXA80_001270"
                     /pseudo
     CDS             complement(2057..2335)
                     /locus_tag="VXA80_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3099..3869)
                     /locus_tag="VXA80_001271"
     CDS             complement(3099..3869)
                     /locus_tag="VXA80_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV0353766.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3884..4732)
                     /locus_tag="VXA80_001272"
     CDS             complement(3884..4732)
                     /locus_tag="VXA80_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV0353767.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4729..6204)
                     /locus_tag="VXA80_001273"
     CDS             complement(4729..6204)
                     /locus_tag="VXA80_001273"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV0353768.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6528..7883)
                     /locus_tag="VXA80_001274"
     CDS             complement(6528..7883)
                     /locus_tag="VXA80_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0353769.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7893..8210)
                     /locus_tag="VXA80_001275"
     CDS             complement(7893..8210)
                     /locus_tag="VXA80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0353770.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8227..8544)
                     /locus_tag="VXA80_001276"
     CDS             complement(8227..8544)
                     /locus_tag="VXA80_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0353771.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPITDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8559..9455)
                     /locus_tag="VXA80_001277"
     CDS             complement(8559..9455)
                     /locus_tag="VXA80_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV0353772.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9458..10720)
                     /locus_tag="VXA80_001278"
     CDS             complement(9458..10720)
                     /locus_tag="VXA80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0353773.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11000..>12169
                     /locus_tag="VXA80_001279"
     CDS             11000..>12169
                     /locus_tag="VXA80_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0353774.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDSNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTIL"
BASE COUNT         4215 a         2431 c         1869 g         3654 t
ORIGIN      
        1 gttgtgatca attattgctg gaggtcttat tggtctcttg gcaggtaaaa tcactaaaaa
       61 agtagttcta tgggaatcat cgcaaatgta ttcgctggtt tagtcggggc atatgcagga
      121 caatctcttt taggtagttg gggtccagca atcgctggaa tggctttgct cccatctatt
      181 gtaggtgcag cgattgtgat tactgtagtg tcattcttta caggtagaaa gtaaactttt
      241 cgccagtaaa gttagcaaac tatttttaaa tcaatgacgg gaaaaatagt ttaaatgtta
      301 aatcgaaagg attgtatatg tcaaaagcaa agaaaatatg tttcattatt ttctgtattt
      361 taatcttgac aattttcctt cctgttttga tagattatca tcaagttagt gatctaggta
      421 ttcatctact tagctggaga cagaactccg tagttgaatt ctatcttgct agatatgtct
      481 tttgggggac agtggttcta tcaactttag ttttattatc cattttagtt gtgatgtttt
      541 atcctaaacg ttacttggaa atccaacttg aaactaaaaa cgatacatta aaattaaaga
      601 attcggcaat cgaaggtttt gttagaagtt tggtgagtga tcatagattg atcaagaacc
      661 caactgttca tgtaaattta cgaaaaaata aatgtttcgt tcatgtagaa ggtaaaattc
      721 ttccttcaga caacatcgct gacagatgcc aaataattca aaatgaaata actaatggat
      781 tgaagcagtt ttttggtatt gagcgtcaag taaaacttga agttgcagta aaaaattacc
      841 aaccaaaacc tcaaaacaaa aagactgtta gtcgtgtgaa gtaaggaagt aaaaaatgga
      901 atggcttaaa caatatcgat atccaattat cgctggtctc ataggcgtat ttctggcttg
      961 tttgattgtc tcctttggct tcttcaaaac aatatttgta ttgattttag gagcactggg
     1021 agttgcagct ggattatata tcgaaaaaaa ctatatagat aaataaaaaa ataaaaatta
     1081 ctaatttaat taaaggagtt tcatatgtca aacgaaaaaa acacaaacac taacgtagaa
     1141 aagaaagatg ctactgttgt agctcacgaa atcaaagggg aacttactta cgaagataaa
     1201 gttatccaaa aaatcattgg tctttcacta gaaaacgttt caggtctttt gggaatcgat
     1261 ggtggtttct tctcaaatct taaagaaaaa atcgttaaca gcgatgacgt aacaagtggt
     1321 gttaacgtag aagttggtaa aacacaagtt gcagttgact taaacgttat tgttgagtac
     1381 caaaaaaatg ttccagcttt atattcagaa atcagagaaa tcgtatcttc agaagttgct
     1441 aaaatgactg acttggaaat tgttgaaatc aacgtaaacg ttgtcgacat caaaactaaa
     1501 gaacagcatg aagcagactc agtaagcctt caagatcgcg tatctgacgt tgctgaatca
     1561 acaggagaat tcacttcaga acaattcgaa aaagctaaat ctggtcttgg atctggtttc
     1621 tcaactgttc aagaaaaagt tagcgaaggt gtagaagctg ttaaaggtgc agcaaatggt
     1681 gtagtatctc acgaaaacac tcgtgtaaac taagataaaa taaatataac aggagaaatt
     1741 atcatgtcag tagaagaaaa attaaatcaa gctaaaggtt ctattaaaga aggtgttggg
     1801 aaagccatcg gtgatgaaaa aatggaaaaa gaaggagcgg ctgaaaaagt tgtttctaaa
     1861 gtaaaagaag tcgccgaaga tgccaaagac gctgtagaag gtgctgtaga aggtgttaaa
     1921 aacatgttga gtggcgacga taaataaggt taaaagttac tttatctttt tggtaacatt
     1981 agtcaaaaga gtctgagtca agatgattct cagaaaacaa aaagctagag attcccaatt
     2041 gcggaactct agctttttaa ttttgcctct ttctcttatt atatttcagc aggttgttgg
     2101 ccatgagtat gaatcccatg tcaattctca cttgacgctt ccctctcaga tgacatctct
     2161 tataacccaa acaaaccttt atctgcccaa agacagattt catatcaatc ttacgtttag
     2221 cgaaaatttg tctacccttg gaagataaaa gtgcctgata ttctttagtt tttaaacact
     2281 ggtaacgttc attcatatac agtccctttt gaggggctga ttcaggttaa taatcgcagt
     2341 caacattgat ttcaaggctg tttgctttct atctcccctt tttcatgata tattttcgta
     2401 caggtgcttc aatagtttct atagtaaaat gaaccaaaaa tagtacacgt tgcggtataa
     2461 tgttcttatg gcatattcat tagattttcg taaaaagttc tctcctattt gtgagcgaac
     2521 aggtatagtc aattgattgt cagtttgaag accaagagtc gtaaggcaaa ggatatggca
     2581 gaatccatcc aagggtggct ggcacaattt ctagtgaacc tctttaaatc tattacgttt
     2641 gactgtggaa aagagttttc aaaatggaaa gatatttcca atcatcacga tagtgagagc
     2701 ttttttgcga atctaggatg tccaagatag cggtgtctca acgaacattc aaatagactt
     2761 ttaaggtgtc atgacttgcc aaagcagaca gatttcaacg aagtcagtca agaattttag
     2821 gctgggcaag aactcaattt caggagaaaa tgaaataaat attcccacaa taaaacgcat
     2881 agtatcaaag tttttccaac acctgatatt atgcattttt taattttaca gactttttcc
     2941 cagcctcaag aaatcacatc ccacctaaat cctttggata ccaaatacct catcaggtta
     3001 ttttctgagt tggtaagact gtctagctta atttgacaaa tggagggcgt taaatccagt
     3061 ttttacagga agtctattta aatattttca cactgacctt acaaaatatg aaataaatta
     3121 tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct tacccaattt
     3181 tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga agcaagaaaa
     3241 ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac aattgtagcc
     3301 aaatgagaca gctgggaaaa gaaaataatt gcaagagaat acatcgttgc ttcttttatc
     3361 gaaatagaaa aaaacaccat caataaagat acattgagcg gacctccacc aatccctaaa
     3421 aaactagaaa tcaagcccaa tagaagacca caaatcatat agaatcctat cttatcaaat
     3481 tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtagcatcac aaatagtaaa
     3541 atcatttgaa caatggatac tagccgtact gataattgag ataacaaaac ttgaaaaata
     3601 agatgaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat caatctccat
     3661 tttatctctt tactttgtga aaatcttttg gttgttgaaa taatcgccat tataaaaaca
     3721 gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact atccataagt
     3781 ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact agctattata
     3841 atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct ttcaaacatc
     3901 taaaacctag atggctacta gtggaagtag aaatacatcc atttctagca gctactcggt
     3961 atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca tactcctcat
     4021 cttgtatccc atattttggc aactcatagt catcttcatt aaaactagct aataaggtat
     4081 atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga ccaaaatcat
     4141 taggtaaaaa ttcatagaca ggagccgttc ctataaatcc atctaagcca gtattttcat
     4201 aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa agagtatctc
     4261 cccaaggata ttcagattgt cttcctcctc tagcagcgta ttcccactgt gcttcagtcg
     4321 gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca acatgaacta
     4381 ctggatggtc ttctaaaccc actagattac tgttctcacc gtagggatgt ttccagcaag
     4441 cgttcggaac ttgtaaccac cacggagccc cggcaggatg aggatatccc tccctttctg
     4501 cctccggaac gaacaatata aaaacaaaag accactcttg tcgctcagct agtgttttat
     4561 agcctgtttc tttcacaaat tgagcaaatt cttgattcgt aactggagta tccgcaatgg
     4621 aaaaatcctt tacactaaca gcgacttgag gatcttcaaa atcatctaaa aagccctctt
     4681 cagaatttgt tcccatcata aatgtcccac ccttgatttt tttcattttc atgattgact
     4741 atcccccgtt ttcgttagag ttggcgttat tttagaaagt tcaacaggaa ctaactcttc
     4801 atccacaaca aaaccttctt ctctatatct aagaaagttt actaattttt tcttcatctg
     4861 acgaacaata ggttggtatt tttcacttgg atacaaatca ttcatttcat gcggatcttt
     4921 tttcatatca aataactgat aatgatttaa aacaggaaac cagataaatt tccactggtc
     4981 tgtcaaaata tattgactag aatcttttcc taaagcatgt tcaccatgaa attctgttcg
     5041 ccacccctcg tactgtccaa ataataaatt cttaacactc cgtccatcta gttcatctgt
     5101 agtagttcct cctgccaaat ccacgagtga aggaaaaata tcttgaattt tcactaattg
     5161 cttgattgtt cccctattac cagctatcaa tccagccgga tcgtaaataa acgaaggaat
     5221 gtggatactt ccctgataag gatatccctt tctaaacaga taatgttcac caagctgatc
     5281 tccatgatcc gaaacaaacc agataatcgt atctttatca tgacgaaatt cttttagggc
     5341 agtcaaaaag cgactaattt gatggtctat atgagtaatc aatccaaaat aagcagcaac
     5401 cattcttctt tgatcatctt cctttaattt ccctcttaaa gcataaatag acggaatttg
     5461 cttttctaat acttcccaat tcccgatatg caaatcgaga aattgaggca agcgttccat
     5521 atacatatca aaatagtatt ttgggggatt taatggagca tgtggttttt caaaagacat
     5581 tttgagaaaa aaaggaaccg ttggatctct tctttgtaaa aatgaaatgg actcactcac
     5641 aacccagttt gttggatgta acttttcatc tttatcccaa ggtctagctt cccaagaatt
     5701 acaatccatt ccgtcatcaa tcaaatcaac atcataacca actttaccct ttaaaaaggc
     5761 tagatagtca ctagcataat caaattgact tccataagcc ttatcatact ttctatcaac
     5821 atgtagatac ccatcgtgaa gtaagacatg atcaaaccca agtctctgtc tggaaggaaa
     5881 aacatgcatc ttaccaatac actctgtttg ataacccata tctttaaaga cttttggaag
     5941 agtattagta aaattccaag gcacctcatc ttgataacca actcgtccac ttttatcttg
     6001 gtctaagcca gtcaataaag ctgccctagc aggaacgcaa gaaggaactg gagaataggc
     6061 attttcaaaa ttataaccta cactagccat catatctaat gttggagtgc taactagctt
     6121 atccttacta ttcaaggata aggcgtctgc tctcatttga tctacaacaa tcaaaataat
     6181 atttggctgt tttgtctgaa ccataaaatc tcctttctaa tatggcaaaa gaggcacaag
     6241 aagatatcta cctttactgc acccctttct atatcaatct ctctatataa aacaataaca
     6301 ttcttgttat gttttataga aaaatgaact aaaatatgac taaatcgatt aggaaattca
     6361 aatcattttc tagtactatt ttagaagtta caatgtacta ttccaacttc aataaactat
     6421 aaacctttgt ctaataacaa ttttagtgga gataagaaat cctacaccta actcaatctt
     6481 acacgtaatc tatttctatt ttatcacaaa aaacgcaagt aagaccatta actcaattca
     6541 gttttatctg ccattttcac aaatgggaaa taagtcaaga cactaataat caaacaaaca
     6601 actgataaga tgatggcacg ccaatcaaat gctgtagaga agaaaccata taaaattgga
     6661 ggcattaccc aagtaacatt ttgtgtaaca ggtgaaacaa gaccccagct tgttgcccag
     6721 taagctaccg ttgccatgaa aaccgggcta agtacaaatg gtataaatag caaaggattc
     6781 aagacaactg gtaaaccata atttaccggc tcaccaatat taaacagaac tggtgctaga
     6841 ccaagtttag caacttttcg ataatgactg tttcttgaaa aaattaaaat agcaagtacc
     6901 aatcctaatc ctccaaacca gacaaacgcc ccaaaagacc cacttgtcca tatataagga
     6961 atcggttcac ctttttggaa agcatccaga ttcgctaaca tagcaactcc aaacagccct
     7021 tccatgatgg gagccaatac atttcctcca tggagaccaa aaaaccagaa taacttattt
     7081 aaaaagatca tcagaataac tgcaaagaaa ctttgagaca aacctagtaa tggcgtttgt
     7141 aacaccttgt aaacccaatc aatcaataag tcattgctaa gtaaatggaa aacataagtc
     7201 aagatggcta ctatatacat cgccataaat cctggaatga tagaagtgaa cggcttagca
     7261 atcgcagggg gaactgaatc tggtaacttg attacccagt tctttttcat tactttacag
     7321 aaaataatag aggctaaaaa tccaatcatc atggctgtaa agtagcctct ggcattaata
     7381 tggtttcctg gaatcacatt cccaatagtt accatcagat ttttaccatc aaatgctaga
     7441 ttatcaattc cttgtaagat ttgatctaat ttcacatctc ctacatttgc caaagggaaa
     7501 ctctttgtaa ctgtacttcc aatcgaaatg acaaacgaag caagcgatac caaaccagca
     7561 gaaactgtat caaccttata aatcttagcg atattcactc ccaagcaata gatgaacaac
     7621 aaggaaacaa ttggtatact tcccttgaat accaaattat tgatgtcaac aagccactga
     7681 aaggttttcg taatactttc taggtgaaat tgttgtggta aatccactag aaaagcattt
     7741 aataacaaag caatggaacc tgtcataata acaggcatag tccccacaaa tgaatcacgt
     7801 aaagcaacta aaaacttctg attcccaact ttcattgcaa ttggagaaag tttttcttgc
     7861 attttatcca acattgtatt catactaaac tcctaatttt tttcaaatcg ttcgtatact
     7921 tctaccactt ctttcgccaa gtcaacaaaa gtcagactcg tcattaaatg atcttgcgcg
     7981 tgtaccatca agaggctaat tgatgtttct tccccctgtg cctctcttgt caacatttct
     8041 gtttgagcct tatgcgcctg taataaggca gcattcgcat cagctaatct tcgattggct
     8101 ttagagaaat cccctttttt tgctgcctga atagcctcca tagcattact tttggcttcc
     8161 ccaccataca taattagccc cattacagat tctaaattac tttcatccat aacattctcc
     8221 tcaatcttag tctaataaac tttcagctaa atctaagact ttagaaccat tcatcattcc
     8281 ataatctgtt atcggaataa ccgccacagg aatctgtcta tcttttagtt tttcttgaaa
     8341 gtcccctagt aaatagcgaa cttgaggacc taaaagcaac acatttactt cttttgttgc
     8401 tacaatttct tctgcttcag gagctggaac tgcaaaaatt tctgcatcca accccttatc
     8461 ttctgctgcc ttttgcatct ttgtcactag cagacttgta ctcatacctg ctgcacatgc
     8521 taacataatt gttactttag ccatagctta ctcctttatc acctatttac tgacctagcc
     8581 aagcgagata ctgtatgtct cactcctcta attgccttgc tcaatgcata aatattatcg
     8641 actgttgcca gtcccccata tcctgcgtca cctatatgtt gaatatcaac tccacaaatt
     8701 ttatttctaa gtgcaatttc cttaatagta tctgtatcag atgtttcttg gctagtacca
     8761 atagcactca atacaagacc acccttacta tgaacgagat caacgacttc acgcaactct
     8821 tggtcatgaa aggctggaac ggttccaact gctggaacaa gtatcacatc cgcacctgct
     8881 tccaacaatt gctctgcgac agaaagctct gccacaggct cattcactcc tgccccgtgc
     8941 atcttacctg caataatcaa accagaaaag ttttccttag cagtttgaac agccttaatg
     9001 atttctcgat tgctaactcc tgttccagga tttccagtca aacagacaaa gtcaaaacct
     9061 aactcctcta ttcgcttcaa tgtttcaaca ctagcaacac gacctgcaac aatttcctgt
     9121 gtttcctcta gcatctttgc agatgggtca atcggttcca aatttacacc aattgggcaa
     9181 gcaacgagct ggtgtaagcg atgaataaca tcatcacctg aactatccaa agcataaatt
     9241 ttaggctcaa aaacatcaac acaattcaat aaaatcatat cagctccaaa agctctagca
     9301 atttcagaat tagttatatc cccaacaaat gtctcacgag tgactacatt ttcagataga
     9361 acaactctac cttcacttgc taaaatactt tgttttaatt cttcagcatt catctgtaat
     9421 atctcagatg gatttgcact aattaaacgt ttcattctca atcctccaat aacttaaacc
     9481 gtttttcaat agctattaca aatggtaaat aaagtaagac atccaaaaca atcaatacaa
     9541 tttgtaataa tgaaccagaa atacttcctg tagcaagtaa accacttata acaggtggca
     9601 tagtccaacc tggatctgta taagttagcg gaaccaagcc tgatgccatt gctccccacg
     9661 ctactagtaa gttaaacatt ggagctaaga taaatggaac tagtaacaaa atgttcaaaa
     9721 caataggaac accaaacatt gctggttcat ttatattaaa taatccaggt ataacagtta
     9781 taggtgccaa agtttttagt tgtttactag ctttcttctt atgtgctaaa tatcctaacg
     9841 caagaactaa acctatggtt gcaccaccgc cacctatgaa tacaaaatta tccataaata
     9901 cgctagtaaa aatatttgga agagtttctc cagtttgata agctacttta tttgcatcta
     9961 gattagctaa ccataacggt ttcataactg cattaacaac atttcctccg tgtaatccta
    10021 caaaccaaaa actactatta aggaaaatta agataagaag accaataaca ttatttccta
    10081 aaagtccaag tggacctccc aaagctactt gagcaatttc atgaagatta ggtaaactta
    10141 atttatctaa agttgcataa acaatcaacc aaccaacaat agtcactgca ccaggaataa
    10201 tggcactaaa gcttttagat actgctggtg gaacaccatc cggcattttt atctgtatat
    10261 tatgatttat aaaccattgg tagatatagc cattaatcaa tcctaaaatc atagcaacaa
    10321 ataaaccttt tgatgccatg aaagcggtag gcattcctgc tccagcctct cctgtaatga
    10381 aaggcgtaac tgtaataaaa gatgataatg acacaatacc agcaggaata ccatcaactt
    10441 tgtattgtct agccataaag taagcgatac caaaactagc tacaagacca agtaatccaa
    10501 aactactatc aacacccttc cataaatagt cagcaacacc aatatcaccc agatattttt
    10561 cccatcctgg aataggaaaa cttgcaataa ccattagtaa tgaacctatc aacaataaag
    10621 gtatagttag agtaataccg tctcgaatag aaaccaacgc tttattatta cccaatctag
    10681 ccgctactgg aagtaacttt tcttgtaata atttttccat aaccttttcc tcttaaatat
    10741 attttattat caaaagttat catttatctt tattttttga cgtcaagttg ttatttgatt
    10801 atcttttgtt atttatattt tacaactata agaaagcctt gtcaaggatt tgtgtaaaca
    10861 tttgattatt ttttattatc aatatataat aaaaagagac cagtaagtaa tgaaaagctt
    10921 aaaaagatta acatctacat ttatccatag caaaaaatca taggatttta agaatctgac
    10981 aactaggaag aaaactaata tgaccttatc taagaaacaa ttacaactaa gagctaaaat
    11041 actggaaaca gtttacacat taggtcccat ttcacgaatt gaaatcgcta ctaaaacagg
    11101 aataacacct gcaacaacta gcagtatcac aaatgatctg attaaagaaa atattctctt
    11161 agaacttggt gaagacgaac acgatacaag cgtagggaga aaaaaaatcc tgctagacat
    11221 tcaagctaaa cgattttact acattggttg tgaactatct gaaaaacatt tcacttttgc
    11281 tttaggagat aatctaggaa atatcttaaa ggaagagaaa gaaattgtta ctaaacagtt
    11341 aatccaagaa aagggaaatc aactcattaa ccagacacta aagcaattct taaataactg
    11401 ctctgattat gaaatagaag caattggaat cgcgctacct ggtcgctatt tagatgatta
    11461 taaaataaca acaaacaatc ctttatggca acatatcgac ttagaaatga ttcaatccca
    11521 ctttgataag cctttgtttt tctctaacaa tgttaactgt atggctatag gtaaacgcct
    11581 atttagtcgc caacagaatg actctaattt tgcttatttt cattttgcta gaggtatgca
    11641 ctgttcttat atctacgacg gaaacattta tgggaaagga aatctaatga ttggagaaat
    11701 agggcataca gttgtttcat ctgagggaga agaatgtagc tgcgggcgca agggatgctt
    11761 acaaactttt gctggagaat cttggttaat caaaaaatca aaaattctat atcatcaatc
    11821 tccctattct ctacttccta gtttagtcaa aaatgccgat gatattgata tccaagttat
    11881 tttaaccgct tatcaattag gtgacactgg aataatcaca cttatccatc aagccttact
    11941 ctatcttagt caaaccattt taaatatcag tatgatgatt gactctcaaa aaatctacct
    12001 acatagtcct ttactaacaa atcaacacat catccaaaaa ctatatagtg aaatgaacta
    12061 caaacccaaa ctgctctata atcgcctacc tgaagtcatt attgaacctt acaatgattt
    12121 tacagccgca cactcagcta ttgcactttg tttgtatcat acaattcta
//
LOCUS       DAVKZB010000053        12028 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKZB010000053 DAVKZB010000000
VERSION     DAVKZB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.053"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(146..1777)
                     /locus_tag="VXA80_001280"
     CDS             complement(146..1777)
                     /locus_tag="VXA80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV0353775.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2070..7049
                     /locus_tag="VXA80_001281"
     CDS             2070..7049
                     /locus_tag="VXA80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV0353776.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETASPASNEAATTE
                     TAEAASATKPEEKASEVVAETPSVEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQSDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFALSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTAPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7139..8335
                     /locus_tag="VXA80_001282"
     CDS             7139..8335
                     /locus_tag="VXA80_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV0353777.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8471..8998
                     /locus_tag="VXA80_001283"
     CDS             8471..8998
                     /locus_tag="VXA80_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0353778.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9075..9431
                     /gene="glnR"
                     /locus_tag="VXA80_001284"
     CDS             9075..9431
                     /gene="glnR"
                     /locus_tag="VXA80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV0353779.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9468..10814
                     /gene="glnA"
                     /locus_tag="VXA80_001285"
     CDS             9468..10814
                     /gene="glnA"
                     /locus_tag="VXA80_001285"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV0353780.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMEKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10904..11023)
                     /locus_tag="VXA80_001286"
     CDS             complement(10904..11023)
                     /locus_tag="VXA80_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV0353781.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11513..12028
                     /locus_tag="VXA80_001287"
                     /pseudo
     CDS             11513..12028
                     /locus_tag="VXA80_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3863 a         2204 c         2578 g         3383 t
ORIGIN      
        1 ctaacaatgt tttagaaata gaggtgtact attctagttt caatatacta tatatctgca
       61 gaatactctt tcagtttcat tcagcctagt cttgcttagg aaaaagtaaa aagccatcca
      121 attatgatgg atagcttctt ggttcttaga tttgttctgc aaaaaccttt tcagcaaggt
      181 tcatagcgtg gtctgataca cgagtgtagt gtgagatgat gtcgataaag ttgaccccag
      241 cttgtgttga acattcgcct ttgttgaggc gtttgatgtg ggtttttctg agaacacgtt
      301 ctatcttatt gattgcttca tgacgttcta caagactgcg tgctttttca atatcattgt
      361 tttccacact atccagagca tctttgataa agtcactagt ttggcggtaa acttcctcta
      421 attctttcaa ggcggcatca gaaaattcaa catttttccg ttgaagatag tcagtcagat
      481 tgagtagagc ctccgtgtgg tctccaatcc gttccaaatc acgggaggaa tcaaggatat
      541 tggtaagcac ttcactttct ttttggctga gagcttcgct tgaaagggca atgagataac
      601 gtgttaattg ctcatcgatg gtgttaattg cttcttcggt tttatgccct ttttcagcaa
      661 ctttttcatc caagtcaatg atgtacttat aggaaaggtc aaaggcttta gcagcgtagt
      721 ttcctaagtg caagagctct ttcttagcat ttcctagagc gatagatggg gcctgtttga
      781 tgaaatgttc atcaagatat aagggttcgt atttgacaac ctcgtcctct ccaggaataa
      841 tcttggttac aaagtaagcc agagctccga taaatggaaa ttggacaatg gtgttggtaa
      901 tattaaaggt tccgtgagca aaggcgatgg tcatttccgg tgctagattt agcgtagctt
      961 caaaccaatg aatcaggaca gtaaaaggaa ctagaaaaat aacgcagacg actgttccga
     1021 taacgttgaa ggcaacatga gctcctgcta cccgtttagc tgcaatatta gcccctaaag
     1081 aggcaatgat ggctgtaatg gttgtcccga tattgtcacc aaatagaacc ggcaaagctc
     1141 cctgtaggtc aattagattg ccggcgtaga ggttttgtaa aatcccaatg gtagccgaag
     1201 aagcttgaat tagcaaggtc aagccagtac cgacaaagac acccaaaaca ggatttttac
     1261 ttagctcaat catatagtcc ttaaagacct gtaaatcctt gagtggagcc attgcgccgc
     1321 tcatgagatt gagggcaaaa aagataccac cgacaccaaa gaggatgcgt ccgatattat
     1381 tgactgtccg attttttgta aaaaagagac agacggcacc gataaagagc ataggtaggg
     1441 catagttacc taatttaaaa ccgatgagaa aggatgtgac agttgtccca atattagcac
     1501 ccatgacaat cccgatagcc tgacgtaagg ttaagagacc ggcactgacc aggccgactg
     1561 tgataactgt tacaccagaa ctagactgaa ttagagcagt catcccaata ccaaccagaa
     1621 ctccaaagaa aggattacta gtatatttgt caatgtaaaa acgaaggcga tctccagcag
     1681 cttgttgtaa accgtctccc atggtcttga tgctatataa gaatagcccc agaccaccta
     1741 aaaagtgaaa taaaatttcc tgccaattaa tggacatttc tttttcctcc gaaaaataat
     1801 cgcggaatat ctcctattct attttaaagg ataaaagtaa atctaacaag tgttaatgtc
     1861 aagtttgtaa aagaaaatta taattgataa gtgtattatt tttaagagtt tttaacctta
     1921 ttttagattt gtgcaaacag ttattgtttt ttgtctaaaa atgttttagg ataaaaatgt
     1981 gattatcatg gttctagagt ttgataaaaa accacagaat ataaagcgct ttcttgaaaa
     2041 caacaaaatc aatcttttag gaggagaaaa tgaagaatcc attttttgaa agacgttgtc
     2101 gttacagtat tcgtaagtta tcagtaggag cctgctcgct gatgattggt gctgttttat
     2161 ttgctggtcc agccttggct gaagaaactg cagttcctga aaatagcgga gctaatacag
     2221 agcttgtttc aggagagagt gagcattcga ccaatgaagc tgataagcag aatgaagggg
     2281 aacatgctag agaaaacaag ctagaaaagg cagaaggagt agcgacagca tctgaaactg
     2341 cttcgccagc aagcaatgaa gctgcaacta ctgaaactgc agaagcagct agcgcaacta
     2401 aaccagagga aaaagcaagt gaggtggttg cagaaacacc atctgtagaa gcaaaaccta
     2461 agtctgacaa ggaaacagaa gcaaagcccg aagcaactaa ccaaggggat gagtctaagc
     2521 cagcagccga agctaataag actgaaaaag aagtccagtc agatgtccct aaaaatacag
     2581 aaaaaacatt aaaaccaaag gaaatcaaat ttaattcttg ggaagaattg ttaaaatggg
     2641 aaccaggtgc tcgtgaagat gatgctatta accgcggatc tgttgtcctc gcttcacgtc
     2701 ggacaggtca tttagtcaat gaaaaagcta gcaaggaagc aaaagttcaa gccttatcaa
     2761 acaccaattc taaagcaaaa gaccatgctt ctgttggtgg agaagagttc aaggcctatg
     2821 cttttgacta ttggcaatat ctagattcaa tggtcttctg ggaaggtctc gtaccaactc
     2881 ctgacgttat tgatgcaggt caccgtaacg gggttcctgt atacggtaca ctcttcttca
     2941 actggtctaa tagtattgca gatcaagaaa gatttgctga agctttgaag caagacgcag
     3001 atggtagctt cccaattgcc cgtaaattgg tagacatggc aaagtattat ggctatgatg
     3061 gctatttcat caaccaagaa acaactggag atttggttaa acctcttgga gaaaagatgc
     3121 gccagtttat gctctatagc aaggaatatg ctgctaaggt aaaccatcca atcaagtatt
     3181 cttggtatga tgccatgacc tataactatg gacgttacca tcaagatggt ttgggagaat
     3241 acaactacca attcatgcaa ccagaaggag ataaggttcc ggcagataac ttctttgcta
     3301 actttaactg ggataaggct aaaaatgatt acactattgc aactgccaac tggattggtc
     3361 gtaatcctta tgatgtattt gcaggtttgg aattgcaaca gggtggttcc tacaagacaa
     3421 aggttaagtg gaatgacatt ttagacgaaa atgggaaatt gcgcctttct cttggtttat
     3481 ttgccccaga taccattaca agtttaggaa aaactggtga agattatcat aaaaatgaag
     3541 atatcttctt tacaggttat caaggagacc ccactggcca aaaaccaggt gacaaagatt
     3601 ggtatggtat tgctaaccta gttgcggacc gtacgccagc ggtaggtaat acttttacta
     3661 cttcttttaa tacaggtcat ggtaaaaaat ggttcgtaga tggtaaggtt tctaaggatt
     3721 ctgagtggaa ttatcgttca gtatcaggtg ttcttccaac atggcgctgg tggcagactt
     3781 caacagggga aaaacttcgt gcagaatatg attttacaga tgcctataat ggcggaaatt
     3841 cccttaaatt ctctggtgat gtagccggta agacagatca ggatgtgaga ctttattcta
     3901 ctaagttaga agtaactgag aagaccaaac ttcgtgttgc ccacaaggga ggaaaaggtt
     3961 ctaaagttta tatggcattc tctacaactc cagactacaa attcgatgat gcagatgcat
     4021 ggaaagagct aaccctttct gacaactgga caaatgaaga atttgctctc agctcactag
     4081 caggtaaaac catctatgca gtcaaactat tttttgagca tgaaggtgct gtaaaagatt
     4141 atcagttcaa cctcggacaa ttaactatct cggacaatca ccaagagcca caatcgccga
     4201 caagcttttc tgtagtgaaa caatctctta aaaatgccca agaagcggaa gcagttgtgc
     4261 aatttaaagg caacaaggat gcagatttct atgaagttta tgaaaaagat ggagacagct
     4321 ggaaattact aactggctca tcttctacaa ctatttatct accaaaagtt agccgctcag
     4381 caagtgctca gggtacaact caagaactga aggttgtagc agtcggtaaa aatggagttc
     4441 gttcagaagc tgcaaccaca acctttgatt ggggtatgac tgtaaaagat accagcctac
     4501 caaaaccact agctgaaaat atcgttccag gtgcaacagt tattgatagt actttcccta
     4561 agactgaagg tggagaaggt attgaaggta tgttgaacgg taccattact agcttgtcag
     4621 ataaatggtc ttcagctcag ttgagtggta gtgtggatat tcgtttgacc aagccacgta
     4681 ccgttgttag atgggtcatg gatcatgcag gggctggtgg tgagtctgtt aacgatggct
     4741 tgatgaacac caaagacttt gacctttatt ataaagatgc agatggtgag tggaagctag
     4801 ctaaggaagt ccgtggtaac aaagcacacg tgacagatat cactcttgat aaaccaatca
     4861 ctgctcaaga ctggcgcttg aatgttgtca cttctgacaa tggaactcca tggaaggcta
     4921 ttcgtatcta taactggaaa atgtatgaaa agcttgatac tgagagtgtc aatattccga
     4981 tggccaaggc tgcagcccgt tctctaggca ataacaaggt acaagttggc tttgcagatg
     5041 taccggctgg agcaactatt accgtttatg ataatccaaa ttctcaaact ccgctcgcaa
     5101 ccttgaagag cgaagttgga ggagacctag caagtgcacc attggatttg acaaatcaat
     5161 ctggtcttct ttattatcgt acccagttgc caggcaagga aattagtaat gtcctagcag
     5221 tttccgttcc aaaagatgac agaagaatca agtcagtcag cctagaaaca ggacctaaga
     5281 aaacaagcta cgccgaaggg gaggatttgg accttagagg tggtgttctt cgagttcagt
     5341 atgaaggagg aactgaggac gaactcattc gcctaactca cgcaggtgta tcagtatcag
     5401 gttttgatac gcatcataag ggagaacaga atcttactct ccaatatttg ggacaaccgg
     5461 taaatgctaa tttgtcagtg actgtcactg gccaagacga agcaagtccg aaaactattt
     5521 tgggaattga agtaagtcag gaaccgaaaa aagattacct agttggtgat agcttagact
     5581 tgtctgaagg acgctttgca gtggcttata gcaatgacac catggaagaa cattccttta
     5641 ctgatgaggg agttgaaatt tctggttacg atgctcaaaa gactggtcgt caaaccttga
     5701 cgcttcatta ccaaggccat gaagttagct ttgatgtttt ggtatctcca aaagcagcat
     5761 tgaacgatga gtacctcaaa caaaaattag cagaagttga agctgctaag aacaaggtgg
     5821 tctataactt tgcttcatca gaagtaaaag aagccttctt gaaagcaatt gaagcggccg
     5881 aacaagtgtt gaaagaccat gaaactagca cccaagatca agtcaatgac cgacttaata
     5941 aattgacaga agctcataaa gctctgaatg gtcaagagaa atttacggaa gaaaagacag
     6001 agcttgatcg cttaacaggt gaggttcaag aactcttggc tgccaaacca aaccatcctt
     6061 caggttctgc cctagctccg cttcttgaga aaaacaaggc cttggttgaa aaagtagatt
     6121 tgagtccaga agagcttaca acagcgaaac agagtctaaa agatctggtt gctttattga
     6181 aagaagacaa gccagcagtc ttttctgata gtaaaacagg tgttgaagta cacttctcaa
     6241 ataaagagaa gactgtcatc aagggtttga aagtagagcg tgttcaagca agtgctgaag
     6301 agaagaaata ctttgctgga gaagatgctc atgtctttga aatagaaggt ttggatgaaa
     6361 aaggtcaaga tgttgatctc tcttatgctt ctattgtgaa aatcccaatt gaaaaagata
     6421 agaaagttaa gaaagtattt ttcttacctg aaggcaaaga ggcagtagaa ttggcttttg
     6481 aacaaacgga tagtcatgtt atctttacag cacctcactt tactcattat gcctttgttt
     6541 atgaatctgc tgaaaaacca caacctgcta aaccagcacc acaaaacaca gtccttccaa
     6601 aacctactta tcaaccgact tctgatcaac aaaaggctcc taaattggaa gttcaagagg
     6661 aaaaggttgc ctttcatcgt caagagcatg aaaatactga gatgctagtt ggggaacaac
     6721 gagtcatcat acagggacga gatggactgt taagacatgt ctttgaagtt gatgaaaacg
     6781 gtcagcgtcg tcttcgttca acagaagtca tccaagaagc gattccagaa attgttgaaa
     6841 ttggaacaaa agtaaaaaca gcaccagcag tagtagctac acaggaaaaa ccagctcaaa
     6901 atacagcagt taaatcagaa gaagcaagca aacaattgcc aaatacagga acagctgatg
     6961 ctaatgaagc cctaatagca ggcttagcca gccttggtct tgctagttta gccttgacct
     7021 tgagacggaa aagagaagat aaagattaaa tatcgaaaaa tcttgtgaaa tctttcctta
     7081 tatttccaaa gtgtgatata atagtttcga ataaaataaa taaaggagtt tttgtaacat
     7141 ggcaaaactt actgttaaag acgttgactt gaaaggtaaa aaagtcctcg ttcgtgttga
     7201 cttcaacgta ccattgaaag atggcgtaat cactaacgat aaccgtatca cagcagctct
     7261 tccaactatt aagtacatca tcgaacaagg tggacgtgca attcttttct ctcaccttgg
     7321 acgtgtgaaa gaagaagctg ataaagctgg taaatcactt gctcctgtag cagctgactt
     7381 ggcagcaaaa cttggtcaag atgttgtttt cccaggtgtc actcgtggtg ctgaattgga
     7441 agcggcaatc aacgctcttg aagatggaca agttctcttg gttgaaaaca ctcgttacga
     7501 agatgttgac ggcaagaaag aatctaaaaa cgatcctgaa cttggtaaat actgggcatc
     7561 acttggagat ggtatcttcg taaacgatgc attcggtaca gctcaccgtg cacacgcatc
     7621 taacgttggt atctcagcaa acgttgaaaa agcagttgct ggtttccttc ttgaaaacga
     7681 aattgcctac atccaagaag cagttgaaac tccagaacgt ccattcgtgg ctatccttgg
     7741 tggttcaaaa gtttcagaca agatcggtgt tatcgaaaac ttgcttgaaa aagctgataa
     7801 agtccttatc ggtggtggga tgacttacac attctacaaa gcacaaggta tcgaaatcgg
     7861 taactcactt gtagaagaag acaaattgga tgttgcgaaa gctcttcttg aaaaagcaaa
     7921 tggtaaattg atcttgccag ttgactcaaa agaagctaac gcatttgctg gttacactga
     7981 agtgcgtgac actgaaggtg aagcagtttc tgaaggcttc cttggtcttg acatcggtcc
     8041 aaaatctatc gccaaatttg acgaagcttt gactggtgcc aaaacagttg tatggaacgg
     8101 acctatgggt gtatttgaaa acccagattt ccaagctggt acaatcggtg tgatggacgc
     8161 tatcgtgaaa caaccaggag ttaaatcaat catcggtggt ggtgactcag ctgccgcagc
     8221 gattaacctt ggccgtgcag acaagttctc atggattagt acgggtggtg gagcatcaat
     8281 ggaacttctt gaaggtaagg ttcttccagg acttgcagcc ttgacagaaa aataagattt
     8341 tataaacaaa tcaaagaaga gagggatgaa agttcctctt ttcttttgct taaaataaaa
     8401 acgcttcctc tcaactatta ctcataaaaa tcaccgattt atgataaaat ggaaatagaa
     8461 agttgagatt atgagttatt ttaaaaaata taaattcgat aaatcccagt tcaaacttgg
     8521 tatgcgaacc tttaaaacag gtattgctgt ttttctagtt ctcttgattt ttggcttttt
     8581 tggctggaaa ggtcttcaaa ttggtgcttt gacagccgtt tttagcctga gggagagttt
     8641 tgatgagagt gttcattttg ggacttcgcg tattctagga aatagtatcg gtggactcta
     8701 tgccttggtc ttcttcttat taaatacctt tttccacgaa gccttttggg tgaccttggt
     8761 agttgttcca atctgcacca tgttaaccat tatgacaaat gtagccatga ataacaaagc
     8821 aggggttatt ggtggtgtag cagctatgtt aatcattacc ctatcaattc caagtggtga
     8881 gacaatttcg tacgtgtttg tgcgtgtatc agaaacgttt atgggagttt ttgtcgcaat
     8941 tatcgtaaat tacgatattg atcgtattcg tctcttttta gagaaaaaag aaaaataatg
     9001 ttacatttta taacattatc aattgacgtt tgtctttttt tagactataa tagacagaaa
     9061 gaaggaaatt gtaaatgaag gaaaaagaat ttcgccgaaa tatggctgtt tttcctatcg
     9121 gcagtgttat gaagttgacc gatctatcgg cgcgtcagat tcgttattat gaagatcaag
     9181 agttgatcaa gcccgatcga aacgaaggaa atcgtcgcat gtattccttg aatgacatgg
     9241 atcgtctgct tgaaatcaaa gattatatct ctgaaggtta taatatcgct gccattaaga
     9301 aaaaatatgc tgaacgtgaa gcgaaatcca agaaagcggt tagtcagact gaagtacgtc
     9361 gtgcacttca caatgaactc ctccaacagg ggcgttttgc ttcagtacag tcaccttttg
     9421 gtcgcggtta ggcaaccgca agtagtcata gattaaggag aaaacttatg ccaatcacag
     9481 ctgcagatat tcgtcgtgaa gtcaaggaaa aaaatgttac ctttattcgt cttatgttct
     9541 cagatatttt gggaaccatg aaaaacgtcg aaattcctgc tacagatgaa cagttagata
     9601 aggtcttgtc gaacaaggtt atgtttgatg gatcttctat tgaaggtttt gtacgtatca
     9661 atgagtcgga tatgtacttg tacccggact tggatacatg gacagtcttc ccttggggag
     9721 atgaaaatgg aagtgttgca ggtctgatct gtgatgtcta tacaacagaa ggtgaaccat
     9781 ttgcgggtga ccctcgtggt aatttgaaac gagctcttcg tcacatggaa gaagttggat
     9841 tcaaatcctt caaccttggt ccagagccag aattcttcct atttaagttg gatgaaaatg
     9901 gggacccaac acttgaagtg aatgacaagg gtggctactt tgatttggca cctactgacc
     9961 ttgcggacaa cacacgtcgt gagattgtga atgtcttgac caaaatggga tttgaagtag
    10021 aagcgagtca ccacgaggtt gcggttggac agcatgagat tgactttaag tacgatgaag
    10081 ttctccgtgc ttgtgataag attcaaatct ttaagcttgt tgttaaaacc attgctcgca
    10141 aacacggact ttacgcaaca tttatggaga agccaaaatt tggtattgct ggatcaggta
    10201 tgcactgtaa tatgtccttg tttgatgcag aaggaaataa cgccttcttt gatccaaatg
    10261 atccaaaagg aatgcagttg tcagaaacag cttaccattt cctaggcggt ttgatcaagc
    10321 atgcttacaa ctatactgcc atcatgaacc caacagttaa ctcatacaaa cgtttggttc
    10381 caggttatga agcgcctgtt tacattgctt gggctggtcg taaccgttcg ccacttgtgc
    10441 gcgtacctgc ttcacgtggt atgggaactc gtcttgagtt gcgttcagtg gatccaatgg
    10501 cgaaccctta cattgctatg gctgttcttt tggaagttgg tttgtatggt attgaaaata
    10561 aaatcgaagc accagctcct atcgaagaaa atatctacat catgacagca gaagagcgca
    10621 aggaagctgg tattacagac cttccatcaa ctcttcacaa cgctttgaaa gctttgacag
    10681 aagatgaagt ggttaaagct gctctcggag atcacatcta cactagcttc cttgaagcca
    10741 aacgaatcga atgggcaagt tatgcaacct tcgtttcaca atgggaaatt gataattatt
    10801 tagaccttta ctaatataga agaaagattg cctgtgggta aacagtcaca cagcagtcta
    10861 agccatcaaa tacctataac ccttaaaata gcgatttcgg tcgctattta tttttcgaaa
    10921 aatcctcttg actcttttgc gaattagatt tatcatcaga cgtgtagaag aaaattaagg
    10981 agtcactatg gaaaaattca atttcaaaaa caatatcggg caagaaaaca agttacttca
    11041 aatcgaaatc tacaagttta ctaacttttg caagcttcaa aattacacat ctgtaaatat
    11101 tttttcgaaa gatatatttg aagccatagt aaattgagac tagaatagta cactacatct
    11161 gctaaaaatt tctagaaact aatttaactt tcctaatcga tttgtttaca tcttatttca
    11221 atctactata attatctcaa taaatgatga tggcgactgt tttgtttgtg cttgtcgatt
    11281 tttgaatccc tgatcgagcg gaagaatatt tctgccgagg ttgccaaggg gaaaatactg
    11341 aagatattga attttatgat gaacaattac aggcagaaaa ggtggaggtt ttgaatattc
    11401 gattggaagt aaaaaattga attatctaca atctcatcaa caataaacct atttcagccc
    11461 cattatctat aggcgtattt ttacgccttt ttatatactt gaagcacata agaaacatta
    11521 tgggaagttt gctgatggaa gtactcaaga aattgatgtt ccttatgata ttcctgatac
    11581 ttgggagtgg gtgaggataa aatcaattta ttggaatttt gggcaaaata agccagagaa
    11641 atcctttagg tatatagata cgtctagtat tgatagaaaa aagaacataa tcaactacaa
    11701 aaatctacaa tatctttcac ctgaacaagc gccttcccgt gctagaaaat tagtttcgca
    11761 gaatagtgtc ttattttcaa cagttagacc atatctaaaa aatattgctg tagttagaga
    11821 acttaaagag tatttgatag ctagtacagc atttattgtt ttggatactt tacttaacga
    11881 aacatatttg aaatattatt tattatcaga taattttatc aatcgtgtta ataataaatc
    11941 tactggaaca agttatcctg caatcaatga ttataatttt aatctattat taattgctct
    12001 ccccccacta tccgaacaac aacgaata
//
LOCUS       DAVKZB010000054        11716 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKZB010000054 DAVKZB010000000
VERSION     DAVKZB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.054"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            16..86
                     /locus_tag="VXA80_001288"
     tRNA            16..86
                     /locus_tag="VXA80_001288"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Gly,seq:tcc)
     gene            122..195
                     /locus_tag="VXA80_001289"
     tRNA            122..195
                     /locus_tag="VXA80_001289"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ile,seq:gat)
     gene            202..289
                     /locus_tag="VXA80_001290"
     tRNA            202..289
                     /locus_tag="VXA80_001290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Ser,seq:gct)
     gene            complement(496..933)
                     /locus_tag="VXA80_001291"
     CDS             complement(496..933)
                     /locus_tag="VXA80_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0353782.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEA
                     TNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            1127..1624
                     /locus_tag="VXA80_001292"
     CDS             1127..1624
                     /locus_tag="VXA80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV0353783.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            1624..3279
                     /gene="dnaX"
                     /locus_tag="VXA80_001293"
     CDS             1624..3279
                     /gene="dnaX"
                     /locus_tag="VXA80_001293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV0353784.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTNLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPERFEFLADKVKVEED"
     gene            3307..3501
                     /locus_tag="VXA80_001294"
     CDS             3307..3501
                     /locus_tag="VXA80_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV0353785.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            3651..4421
                     /gene="sufC"
                     /locus_tag="VXA80_001295"
     CDS             3651..4421
                     /gene="sufC"
                     /locus_tag="VXA80_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV0353786.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            4449..5711
                     /gene="sufD"
                     /locus_tag="VXA80_001296"
     CDS             4449..5711
                     /gene="sufD"
                     /locus_tag="VXA80_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV0353787.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            5722..6948
                     /locus_tag="VXA80_001297"
     CDS             5722..6948
                     /locus_tag="VXA80_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0353788.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6935..7375
                     /locus_tag="VXA80_001298"
     CDS             6935..7375
                     /locus_tag="VXA80_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV0353789.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            7429..8841
                     /gene="sufB"
                     /locus_tag="VXA80_001299"
     CDS             7429..8841
                     /gene="sufB"
                     /locus_tag="VXA80_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV0353790.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(9072..10313)
                     /locus_tag="VXA80_001300"
     CDS             complement(9072..10313)
                     /locus_tag="VXA80_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV0353791.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            10416..11525
                     /locus_tag="VXA80_001301"
     CDS             10416..11525
                     /locus_tag="VXA80_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0353792.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3436 a         2128 c         2547 g         3605 t
ORIGIN      
        1 attgatagta tttatgcggg tgtagtttag tggtaaaact acagccttcc aagctgttgt
       61 cgcgagttcg attctcgtca cccgctttga actttgttca aattaccaag ttttttaact
      121 tgggcgcgta gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc ggtggttcga
      181 gtccactcgt gcccattaat tggagaatta ctcaagaggc tgaagaggac ggtttgctaa
      241 atcgttaggt cgggtaactg gcgcaagggt tcgaatccct tattctccgt tttaatgaga
      301 gtttgtaact ctctttttta tttttagaga caataaagct tcagatccgt aaaacttaat
      361 tgaattacta atctgaagtt atttttatgg ctctttgtca actgtagtgg gttgaagaaa
      421 agctaagctc gagcaaggac aaatttcgtc ctttcttttt tggctctttg tcaactgtag
      481 tgggttgaaa aaaagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatgtt
      541 caaagcgata aaaatccttt ttttgaagtt ttcaaagttc cgaaatccaa aggcgtttcg
      601 tttgataagt ttgatgagat tatttgtcgc ttccagtttg gcgttagaat agtgtagttg
      661 aagggcgttg acaatctttt ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat
      721 aggatgaacc tgcttcagat tgtcctcaat aagcccgaaa aatttctctg gttccttatt
      781 cttcattaga cttccacaat cagggcaaga tggagcctca taatccagct tagcgataat
      841 ttctttgtgg gtatccatat tgatgatatc tagaatcttg atgtttgggt ctttaatatc
      901 gagcagtttt gtgataaaat gtaattgttc catatgaatc tttctaatga gttgttttgt
      961 cgcttttcat tataggtcat atgggacttt ttttctacaa caaaataggc tccataatat
     1021 ctatagggga tttacccact acaaatatta tagagcccaa aatcctttgt ttactaaaca
     1081 agggattttt cttttgtctc tgctcctttt ttgatataat agttctatgt taaaatcaga
     1141 aaaacaatca cgttatcaaa tgttaaatga agaattgtcc ttcctattgg aaggcgaaac
     1201 caatgttttg gctaatcttt ccaacgccag tgctctcata aaatcacgtt ttcctaatac
     1261 cgtatttgca ggcttttatt tgttcgatgg aaaggaattg gttttaggtc ccttccaagg
     1321 aggtgtttcc tgcatccgta ttgcactagg caagggtgtt tgtggtgagg cagctcactt
     1381 tcaggaaact gttattgttg gagatgtgac gacctatctc aactatattt cttgtgatag
     1441 tctagctaaa agtgaaattg tggtgccgat gatgaagaat ggtcagttac ttggagttct
     1501 ggatctggat tcttcagaga ttgaggatta cgatgctatg gatcgagatt atttggaaca
     1561 atttgtcgct attttgcttg aaaagacagc atgggacttt acgatgtttg aggaaaaatc
     1621 ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa acttctccca gttagttggt
     1681 caagaagttg tggctaagac tcttaaacaa gcggtggagc aagagaaaat aagtcacgct
     1741 tatctttttt ctggtcctcg tggaacggga aaaaccagtg ttgctaaaat ctttgccaag
     1801 gctatgaact gtcccaatca agtgggtggc gaaccttgca ataactgcta tatttgtcaa
     1861 gcagtgacgg acggtagttt agaagatgtc attgaaatgg atgcagcttc taataatggg
     1921 gtagatgaaa ttcgcgaaat tcgtgataaa tctacctatg cgcctagcct tgctcgttat
     1981 aaggtttata tcatagatga ggttcacatg ctgtctacag gagcttttaa tgccctccta
     2041 aagacgctgg aagaaccaac acagaatgta gtctttattt tggccactac tgaattgcac
     2101 aagattcctg ctactattct atcccgtgtg caacgttttg agtttaaatc aattaagaca
     2161 caggatatta aggaacatat tcactatatc ttagaaaaag aaaatatcag ttctgaacca
     2221 gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa tgcgggacgc cttgtctatt
     2281 ttggatcaag ccctgagttt gacacaggga aatgagctga cgactgctat ctctgaagaa
     2341 attactggca ccattagcct atcagccttg gatgattatg tggcggcctt gtctcaacag
     2401 gatgttccca aagctttgtc ttgcttgaat cttctttttg acaatggtaa gagcatgact
     2461 cgttttgtga ccaatctttt gcactattta agagacttgt taattgttca aacaggggga
     2521 gaaaatactc atcatagttc agtctttgta gaaaatttgg cacttcctca aaaaaatctg
     2581 tttgaaatga ttcgcttagc aacagtgagt ttagcagata ttaagtctag tttgcagccc
     2641 aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa tcaagtccga accagctcta
     2701 tcaggagcgg ttgaaaatga aattgctacg ctgagacagg aagttgcccg tctcaaacaa
     2761 gagctttcta atgtaggtgc ggttcctaaa caagttgcac cagctcctag tcgaccagct
     2821 acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc aatctatctt acaagaggcc
     2881 gtcgaaaatc ctgatttagc acgtcaaaat ctaattcgtt tgcagaatgc ctggggagag
     2941 gtaattgaaa gtctaggtgg gccggacaag gctctgctag ttggttctca accggttgct
     3001 gccaatgaac accatgctat tcttgctttt gagtctaact tcaatgctgg tcaaactatg
     3061 aaacgagaca atctcaatac catgtttggt aatatcctca gtcaggcggc aggtttttca
     3121 cctgagattt tagctatttc catggaggaa tggaaagaag ttcgcgcagc cttttcagcc
     3181 aaagccaaat cttctcaaac tgaaaaggaa gtagaagaaa gcctgattcc agaaagattt
     3241 gaatttttgg ctgataaagt gaaggtagag gaagactaaa gaaagatttc atgatacaat
     3301 aagtttatga ataaacaaca atttattatt atggcgctgt ttacagctgc tgagacctat
     3361 tttttcaatg aagcctggat gactggccgc tatattatgg cagccttttg ggcaatttta
     3421 ctctttagaa atttccgagt cagttatgtg atgggcaaaa tcgttgatgt catcgatcag
     3481 cattttaata ggaaagacta gccctcagct tccagacaaa atcaaagcct tttaggcttt
     3541 tttttgttat actagaaaag tatatttata gaatttttgc tctatttctg gggaaatcag
     3601 acgtttttct agtaagtact gtaaaagttt tgaaaaagaa aggaactatc atgtcagtat
     3661 tagagatcaa agatcttcac gttgagattg aaggaaaaga aattttaaaa ggggttaacc
     3721 tgaccctgaa aacaggagaa attgccgcta tcatgggacc aaatggtaca ggtaaatcga
     3781 ctctttctgc cgctatcatg ggaaatccaa actatgaagt aactaaaggt gaagttttgt
     3841 ttgatggcgt aaacatcctt gagttggaag tggatgagcg tgcgcgtatg ggacttttcc
     3901 ttgctatgca atacccatca gaaatccctg gaattaccaa tgctgagttt cttcgtgccg
     3961 ctatgaatgc gggtaaagaa gatgatgaga agatttcagt tcgtgagttt attactaagc
     4021 tagatgaaaa aatggaattg ctcaacatga aagaagaaat ggcagagcgt tacctcaacg
     4081 aaggcttctc tggtggtgag aaaaaacgca atgaaattct tcaacttttg atgttggagc
     4141 caacatttgc tcttttggat gagattgact caggtcttga tattgacgct cttaaagttg
     4201 tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg tgctatgatc attactcact
     4261 accaacgtct tttgaactat atcacacctg atgtggtaca cgtgatgatg gaaggtcgtg
     4321 ttgtcctttc tggtggtcca gaattggctg cgcgtttgga acgtgaagga tacgcaaaat
     4381 tagctgaaga acttggctac gactacaagg aagaattgta atttcctcgt atcttttagg
     4441 agaagtaaat gactagagaa aatattaaac ttttttcaga aatgcacgct gaaccaagct
     4501 ggttggctga tctccgtcaa aaagcttttg acaagattga gactttggaa ttaccagtta
     4561 ttgagcgtgt caaattccac cgttggaatc tgggtgatgg aacgattaca gaaaatgagc
     4621 catcagcaaa tgttccagat ttcacagctt tagatcatca cttgaagttg gtgcaagtag
     4681 gaactcaaac tgttttcgaa caaactccag ttgagttagc tgaacagggt gttgtcttca
     4741 cagactttca ctcagcttta gaagaaattc cagagctgat cgaagaattc ttcatgtcat
     4801 ctgttaagta tgatgatgac aagttggcgg cttaccacac agcttacttt aacagtggtg
     4861 ctgtactcta tattccagat aacgtagaaa tcacagagcc aattgaagga attttctacc
     4921 aagatagcga tagcaatgtg ccgtttaaca agcatattat gattatcgct ggtaaaaatt
     4981 ctaagattag ttatctggag cgtttagagt cacgcggtga aggaagtgac aaagtaactg
     5041 ccaatatcac agtggaagtg attgcacgtt ctggtgcgca agtcaagttt gctgctatcg
     5101 accgtctagg tgaaaacgtc actgcctaca ttagccgtcg tggtaaatta ggcaacgatg
     5161 caagtattga ctgggctatc ggtgtcatga acgaaggaaa tgtcgttgct gattttgata
     5221 gtgacttgat tggtaatggt agccatgctg acctcaaggt tgtagctctt tcaagtggtc
     5281 gtcaggtaca agggattgat actcgtgtaa ctaactatgg ctgcaactca atcggaaaca
     5341 ttctacaaca tggggttatc cttgaaaaag caactttgac tttcaatggt atcggccaca
     5401 tcatcaaggg tgctaaggga gcagatgcgc aacaagagag ccgtgttctc atgctttcag
     5461 accaagcgcg ttcagatgct aacccaattc ttttgattga tgaaaatgac gtaactgcag
     5521 gccatgcagc ctctattggt caggtagatc cagaagatat gtactacctc atgagtcgtg
     5581 gcttggataa ggcaactgca gagcgtttgg ttgttcgtgg tttccttgga tctgttatcg
     5641 tggagattcc agtcaaggaa gttcgtgatg aaatgattgc aactatcgaa gagaaattgt
     5701 caaaacgcta aggggcagcc tatgttagat gtagaagcga ttcgcaagga ttttccaatt
     5761 ttagatcaga ttgtcaatga tgaacctctg gtctatctgg acaatgctgc gacgacacaa
     5821 aaaccactag tagttctgaa agctattaac agctactatg agcaggacaa tgccaatgtt
     5881 caccgtggtg tccatacctt agcggaacga gcgacagctt cttatgaagc tgctcgtgaa
     5941 accattcgta agtttattaa tgcaggctct acaaaggaag ttctctttac cagaggaacg
     6001 acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa ttctcactga gggagaccag
     6061 gtcttgattt cagtaatgga acaccattct aatatcattc catggcagga agcttgtcga
     6121 aagactggag cagagcttgt ctatgtctat cttaaagacg gtgccttgga tatggaggat
     6181 ttgcgagcta aattgactga taaggttaaa tttgtttccc tagctcatgc ctccaatgtt
     6241 cttggtgtgg tcaatccgat caaggaaatc actcaattag cccaccaagt tggggcaatc
     6301 atggtagtgg atggtgctca atctacacct catatgaaga ttgatgtcca ggacttggat
     6361 ctggactttt tcgccttttc gggtcacaag atggctggtc cgactggtat cggtgtcctt
     6421 tacggcaaag aaaagtatct tgagcaaatg tctccagtag aatttggcgg cgagatgatt
     6481 gattttgtct acgagcaatc tgctagttgg aaggaattgc cttggaaatt tgaggctgga
     6541 acgccaaata tggcaggagc tattggactt gcgactgcag ttgattatct ggaaaagatt
     6601 ggtatggatg ccattgaagc tcatgaacag gaattgattg cgtacgtcta tccaaaactg
     6661 caggcaattg agggattgac catttacggt tctcaggatt tggctcaacg ttcgggtgtt
     6721 attgccttta acctaggtga tctccatcct cacgatcttg cgacggctct ggattatgaa
     6781 ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct tgcttcagta tttggaagtc
     6841 ccagcaacag ctcgtgcaag tttttatatc tacaatacca aggcagattg cgacaaacta
     6901 gtcgatgccc tacaaaagac aaaggagttt ttcaatggca ctttctaaac tagatagcct
     6961 ttatatggca gtggtagcag accattcgaa aaatccacat caccaaggga agttagaaga
     7021 cgctgagcaa atcagtctca acaatccgac ttgtggggat gtcatcaacc tctctgtcaa
     7081 gtttgatgca gaggaccgtt tggaagatat tgcttttcta aattcaggat gcacgatttc
     7141 aactgcttct gctagtatga tgacagatgc cgttttagga aaaaccaaac aagaaatttt
     7201 agaactggcg actatttttt ctgaaatggt tcaagggcaa aaagatgagc gtcaagacca
     7261 acttggagac gcggcattct tgtcaggtgt tgccaaattc cctcaaagaa tcaagtgtgc
     7321 aaccctagct tggaatgccc ttaagaaaac aattgaaaat caagaaaaac agtaagacaa
     7381 gtttcttttg tcttatgaat tattagaaat gaagaaagaa aggatactat ggctgaagaa
     7441 agagtagaac caaaaccaat tgaccttggt gaatataaat ttggtttcca tgacgatgta
     7501 gagcctgtct tatcgacagg aaaaggactc aacgaaggtg ttattcgtga attatctgct
     7561 gctaagggtg agcctgagtg gatgttggag ttccgtttga agtcttatga aaccttcaaa
     7621 aaaatgccca tgcaaacttg gggagcagac ttgtcagaga ttgactttga tgacttaatc
     7681 tactaccaaa aaccatctga caaaccagcc cgttcttggg atgatgtacc tgaaaagatt
     7741 aaagaaacct ttgaacgtat cgggattcca gaagctgaac gtgcttattt agcaggggct
     7801 tctgcccagt acgagtcaga agtggtttac cacaacatga aggaagagtt ccaaaaatta
     7861 ggtattatct ttacagatac agattccgca ctcaaggaat acccagactt atttaaacaa
     7921 tactttgcga agttggtacc gccgacagat aacaagttgg cagccctcaa ctcagcagta
     7981 tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca aggtagatat tccacttcaa
     8041 acttatttcc gtatcaataa cgaaaatata ggtcagttcg aacgtacctt gattatcgtt
     8101 gatgagggag caagcgtcca ctacgtagaa ggatgtacag caccaacata ttcaagcaat
     8161 agcttgcacg ctgccattgt agaaattttt gctttggacg gagcttatat gcgttataca
     8221 actatccaaa actggtctga taacgtctat aacttggtaa caaagcgtgc taaggctcaa
     8281 aaggatgcca ctgttgagtg gattgatgga aacttgggtg ccaaaacgac tatgaaatat
     8341 ccatctgttt accttgatgg agaaggagcg cgtggtacca tgctctctat cgcctttgct
     8401 aatgcagggc aacaccaaga cacgggtgct aagatgattc acaatgctcc acataccagc
     8461 tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa aggttgacta ccgtggacaa
     8521 gtcaccttta acaagaactc taagaaatct gtttcccaca ttgaatgtga taccattatc
     8581 atggatgact tgtcagcatc agatactatt ccatttaatg aaattcacaa ctcgcaagtg
     8641 gctttggaac acgaagccaa agtatctaag atttcagaag agcaattgta ttatctcatg
     8701 agccgtggat tgtcagaatc tgaggcaact gaaatgattg tcatgggatt tgtagaaccc
     8761 tttacaaaag aacttccaat ggaatacgca gttgagctga accgcttgat tagctatgaa
     8821 atggagggat cagttggata aaatttgatt ttatactctt cgaaaatctc ttcaaaccac
     8881 gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt cagtttcatc tacaacctca
     8941 aaacagtgtt ttgagcaacc tgaggctagc ttcctagttt gttctttgat tttgagtatt
     9001 agatttactc aaaatcaagg attttgaaga tgaacttgta tcaaaaaatc gcggtttaaa
     9061 atcgcgattt tttataattt ctcgttaaca aagcggacaa actgattcca ccaaactttt
     9121 aagaagaagg ctttttcaat tttcttgtct gctaccattt cgaaactagg gcgctctgtg
     9181 gtgatgtaac cttgaccaat caagtccttg tcttcataag tcaaatggcc aaccactgtt
     9241 ccagcttcaa gtggtgctgg gattgctttg gaatcaggtg tgaattgaac agattgggaa
     9301 gattgattcc caacacgttc gattagatag atatcctctg gagccactgc agttactgta
     9361 tcttcttttc catcttgtac aggggctttg ctatcttgat aggcatcgcc ttgttgaacg
     9421 attttgcgaa gtgtaaatgt agaagaaata taatccatta gggaagatgt agctgtaaat
     9481 cgagcgtaag gattattgtc ttgatgatct gcatttaaaa caactgtgat gactctcatg
     9541 cctttttcga cagtagtacc aacaaaagac tctccagcct tatctgttgt tcctgttttt
     9601 agcccatcaa aaccaccacg gtaagcaggc ataccttcta acatgtagtt ggttgaagtg
     9661 attgtcattc cagcaaaagt agaagaaggt ttttttgtga tttccaagac ttgtgggtat
     9721 tttttgatga ggttgcgagc aacgatagcg acatcataag cactaagctt attttcctca
     9781 tcttttttag aacctgggta aatgttatcc cctagagttt cattgttaag acctgtcgta
     9841 ttgacaacag tggcatcctg aattccccat tccaagagtt ttgcccgcat catatcgacg
     9901 aaatcttttt ctgagccagc aattttctca gctagggcaa tagcggcgct gttggcacta
     9961 gataccagag ttgcttcaag caactcttcg acagtataat tacgggcctc cataggaata
    10021 ttactggctt cagaatttgt cgtcaattga taaggataat cagaaatatc tacaggagtg
    10081 gagagggtaa tacttccgtt ttccaaagct tcatagacca gataaacagt aatcaatttt
    10141 gttatggaag caatttcgac aggttgcgtt gcatccttct catagagaat tttaccagta
    10201 tttgcctcaa cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc aacagcagta
    10261 gaagcccccc ctaaaagaga gacagttaac aaagttaaaa atattttttt catagtagtt
    10321 ttattctatc ataaagaaaa aaaatattct tgctttaata attcatctgt taagcttttt
    10381 gaaaatatgg taaaataaag tgagggaggt aactcatgtt tcgtagaaat aaattatttt
    10441 tttggaccac agaaatttta ctcttaacca tcatctttta cctatggaga cagatggggt
    10501 ctttgattaa cccttttgtt agcgtgctta atacaattat gattccattt ttattagggg
    10561 gctttcttta ttatttgaca aaccctattg ttactttctt aaataaagtc tgtaaactca
    10621 atcgtttgct tggtatttta attaccttgt gtactttggt ctggggaatg gtcataggtg
    10681 ttgtctatct cttacctatt ttgattaatc agttatctag tttgattata tctagtcaaa
    10741 ctatttatag tcgagtacaa gacttaatca tagacttatc taattatcct gcgctccaga
    10801 atttggatgt agaagctaca attcagcagt taaacttatc ctatgttgat attcttcaaa
    10861 atatcctaaa tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt atcagtactg
    10921 ttttgatttt gattatgact ccagtttttt tggtttattt cttattagat ggacataaat
    10981 tcttgcccat gcttgaaaga acgattctaa agagggatcg cttgcatatt gcaggcttat
    11041 taaagaattt aaatgcgacg attgctcgct atattagtgg agtttcgatt gacgcaatca
    11101 ttataggttg tttggcttat attggctata gtattattgg tttaaaatat gctttagttt
    11161 ttgccatttt ttctggtgta gccaatttaa ttccttatgt ggggccaagt attggtttga
    11221 ttcctatgat catcgcaaat atattcactg atccccatag actgctgatt gcagtgattt
    11281 atatgcttgt tgttcagcag gtagatggca atatcttata tcctcgaatc gtaggaagtg
    11341 ttatgaaggt tcatccaatc acgattttag ttttactttt gttgtcaagc aatatctatg
    11401 gtgtagttgg aatgattgtc gcagtgccaa cctattctat cttgaaagaa atttctaagt
    11461 tcttatcccg tttgtatgaa aatcataaaa taatgaaaga acgagaaaga gaattagcta
    11521 agtaaaagtc aggagaaccc tgatttttct ttactggaag tggcctttag attagaagac
    11581 tgaaaataag ttaaagtctt aaactaattt tcacagctaa gaatagtaga agttaatctg
    11641 ataaaaatcg aaaaaaccag tggaattccg tgtcagggta agttccactg gttttcatag
    11701 tctattaaag ttcgaa
//
LOCUS       DAVKZB010000055        11666 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKZB010000055 DAVKZB010000000
VERSION     DAVKZB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.055"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..991
                     /locus_tag="VXA80_001302"
                     /pseudo
     CDS             1..991
                     /locus_tag="VXA80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1119..2141)
                     /gene="cbpF"
                     /locus_tag="VXA80_001303"
     CDS             complement(1119..2141)
                     /gene="cbpF"
                     /locus_tag="VXA80_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV0353793.1"
                     /translation="MKLLKKMMQVVLATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGALKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKPAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2160..2981)
                     /gene="cbpG"
                     /locus_tag="VXA80_001304"
     CDS             complement(2160..2981)
                     /gene="cbpG"
                     /locus_tag="VXA80_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV0353794.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3062..3301)
                     /locus_tag="VXA80_001305"
     CDS             complement(3062..3301)
                     /locus_tag="VXA80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353795.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3451..3717)
                     /locus_tag="VXA80_001306"
                     /pseudo
     CDS             complement(3451..3717)
                     /locus_tag="VXA80_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3742..4053)
                     /locus_tag="VXA80_001307"
     CDS             complement(3742..4053)
                     /locus_tag="VXA80_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV0353796.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3978..4209)
                     /locus_tag="VXA80_001308"
                     /pseudo
     CDS             complement(3978..4209)
                     /locus_tag="VXA80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(4210..4842)
                     /locus_tag="VXA80_001309"
     CDS             complement(4210..4842)
                     /locus_tag="VXA80_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0353797.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4856..5851)
                     /locus_tag="VXA80_001310"
     CDS             complement(4856..5851)
                     /locus_tag="VXA80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0353798.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5848..6546)
                     /locus_tag="VXA80_001311"
     CDS             complement(5848..6546)
                     /locus_tag="VXA80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV0353799.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6624..7634)
                     /locus_tag="VXA80_001312"
     CDS             complement(6624..7634)
                     /locus_tag="VXA80_001312"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV0353800.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(7618..8625)
                     /locus_tag="VXA80_001313"
     CDS             complement(7618..8625)
                     /locus_tag="VXA80_001313"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV0353801.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8612..9565)
                     /gene="mvaD"
                     /locus_tag="VXA80_001314"
     CDS             complement(8612..9565)
                     /gene="mvaD"
                     /locus_tag="VXA80_001314"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV0353802.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9547..10425)
                     /gene="mvk"
                     /locus_tag="VXA80_001315"
     CDS             complement(9547..10425)
                     /gene="mvk"
                     /locus_tag="VXA80_001315"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV0353803.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10547..11566)
                     /gene="cbpC"
                     /locus_tag="VXA80_001316"
     CDS             complement(10547..11566)
                     /gene="cbpC"
                     /locus_tag="VXA80_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV0353804.1"
                     /translation="MKLLKKMMPIALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIHGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPISEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVQ"
BASE COUNT         3460 a         2697 c         2048 g         3461 t
ORIGIN      
        1 aagccaaaaa cgagacctga agagaggaca gaattagaac gtcttcaagc agaaaatgag
       61 tacctgagag cggagaatgc catcctaaaa aagttaagag aactccgatt gaaggaggaa
      121 aaagagaaag aagaaagaca gaaattgttc aagaattaat gactgagttt tcgttagatc
      181 ttcttctaaa atccattaaa ctagctcgtt cgacctacta ctatcacttg aaacagctag
      241 acaaaacaga taaggaccaa gagcttaaag ctgaaattca atccattttt atcgaacaca
      301 agagaaatta tgcttatcgt cggatttatt tagaactaag aaatcgtgct tatctggtaa
      361 atcataaaag agttcaacac ttgatgaagt actcaattta caagctaaaa cgcgacagaa
      421 acgaaaatat tcttctcata aaggagacgt tggcaagaag gcagagaatc tcattcaagg
      481 ccaatttgaa ggctctaaaa caatggaaca gtgctacaca gatgtgactg aatttgccat
      541 tccagtaagt actcaaaagc tttacttatc accagtttta gatggcttta acagcgaaat
      601 tatcgcctat aatctttctt gttcgcctaa tttagaacaa gtacaaacaa tgttggaaca
      661 ggcattcaca gagaagcact acgagaatac gattctccat agtgaccaag gctggcaata
      721 tcaacacgat tcttatcatc ggttcctaga gagtaaggga attcaagcat ccatgtcacg
      781 taagggtaac agcccagaca atggtatgat ggagtccttc tttgggattc tgaaatcgga
      841 aatgttttac ggttatgaga agtcgtttca gtcgcttaag caattggaac aagctattgt
      901 agactatgtt gattactaca acaataaacg aattaaggta aaactaaaag gacttagccc
      961 tgtgcaatac agaactaaat ccttcggata aattaattgt ctaactgttt gggggcagta
     1021 cagaaaaacc ttgatactat gcgttttttg attgtgaaga cttttatcca cctattatac
     1081 aaagttaagt atccatcaca gtttacaatt agccttcatt acttaaccca ttcaccatta
     1141 tagtttaagt agtaaccacc tactgtggta ttaacagcta aagcacctga atcataggca
     1201 tagtaccagt taccattgac ttggaaccag cctgttttca tatctccatt acttgcattt
     1261 agatagtacc aagtcgaacc ttcctgatac caaccagttg ccatagctcc tgatgaacgg
     1321 agatagtacc atttgttccc aaggttttgc cagccagttc tcatatcacc attttcagca
     1381 tctagatagt accaagttga gccttcctta taccagccag ttgccatagc tcctgatgaa
     1441 cggagatagt accatttatt accaagatgt tgccaacctg tttgcatgat gccagttgct
     1501 ggatctaggt agtaccatgg agcaggtttt agcttctctt catcatacgt aagagggtaa
     1561 tccttaaccc aaccacgtgc tagctctcca accgtcaatc tgttgatgcg agaatcaaag
     1621 ccaccatcct tctgtaaata ataccaatga ccctcttcat aaatccaacc agtttttaaa
     1681 gtatgataac tacgctgatc ttcaaaataa tagactcgtt tctctgcttg gctatcatat
     1741 tcttccccat gatgtttgtt ggtattcgta gcagtttttg cttctaaaac ttgcttgcca
     1801 acaaattctt gtaagacacc atcttgacca aaataaaacc aatctggtct aagagcaatc
     1861 tctattcttg gagaaggacc aatcgtaacc cccttgtgtg gagcaggtat atattgccag
     1921 ccgacaacca tctctccgga taaaggatca aaataatagt acttcccatc tatcactctc
     1981 cagtaggttt cttttagagc cccctttttg tagtaggttc taccattttc ttggacaaac
     2041 tgccatcctt cagaatcatc tgcaaatact gtactggtcc ctagcaaacc gaagaaaaat
     2101 gtggctagta cgacttgcat catttttttc aaaagcttca ttttatatat cctccaacat
     2161 taaatccact caccagatga agcgaagtta taaactttac catcgatagt ttggctacct
     2221 gtaaccattg ctccatttga attgagataa taccagtttc catttacttt ttgccaatct
     2281 gtaagcatct taccggaact gtctaaataa taccaagtat catttatctc ctgccaaccc
     2341 gtttgtttat catgttgtct ataatggtac caactaccat ttattttctt ccatccttca
     2401 agagagtaac ctttaagaac cgaataaata aatggcaaat ttcgttcatt taatttaact
     2461 gcactgttaa tttgattagc tccatctcct aaagtatgca ctcctactac acggtgacta
     2521 gcatcataaa ctgtagatcc actagacccc tctaaagtat caacttggta atccaagaac
     2581 atgccatcat cacttaaaac ttgtttttta tctgtataca tttgatgaat tttaaaatta
     2641 tatgatggat agcctgtgat agtcacagtt attcctgtca aatttttttg actagtagga
     2701 agacccaaag tccctaattt tgcaccaatg ggctcttcta gaattaataa agccaagtca
     2761 tattcccttg catccttaga atttaaattt ctaaattcct tcaaataacg aacttccttt
     2821 actttgatct ttccaaatag ttcttgactt ggactaacag ccggaagaac ataaatatca
     2881 tccgcttctt tcccatagtc atgtctgtag tagttgtgag ccgctgtaat taacacatta
     2941 tctttaataa aattcgcact tcccctccct tctacattca ttccatcggc tactccataa
     3001 tacttagata aaaccatagc tgaagtcgaa tacggatact gtaaagtatt atcaatttta
     3061 atcaaatcat cattaccgat aatacttctg attgcttttg gtagtatgaa ccatacgttg
     3121 gtgaaatctc agataatgaa gaatcattag accctggacc tttttctagt gtctcactta
     3181 cctcatattc ttcacctgta ctagaaataa cactcaaagc agatactgtc gataactggc
     3241 tagccaataa agtactcgca ataattgaaa tacccaattt tttataaaca gttttcttca
     3301 ttatcgtatc ctcctaatgt aattatagtg tactattcta aatttctcaa tctactatag
     3361 aatcaagaaa tctaccacct tctttaaata ccctccatta tcacataaac aggtaaactt
     3421 ttcaattaat gactgcgctt ttcaatcacg ctagaggtac ttgcttgctt ctttgatact
     3481 aagttcagcc attctttcct tgtttttctc aataaagcat gttacccaag tgggattcgt
     3541 tttggagtag tctcgcagag tccagccaat ggctttattg ataaaaaatt ctgtttggtt
     3601 caagttatga aggagaatct tttccattaa ttgagtattg gtcttctctt ttcttaacaa
     3661 ctggtggtca atagcgacac gtctcagcca aatattatct aataggctcc attttatact
     3721 caatgaaaat caaagagcaa actaggaagc tagccgcaga ttgctcaaaa cactgttttg
     3781 aggttgcaga tagagctgac gtggtttgaa gagattttcg aagaatatta agattatttc
     3841 ttctagttca gggtgtttat acaccaaact ccctactact cgatctagga tatctaccgt
     3901 gtcccaccag gattttgtca cgactaactg ctctagctta ggcaaatcgg tttcctttag
     3961 ataagactgc attgctttca aatagttagc agccacatat tggtattttc taggctcctt
     4021 ttcccagcaa gtgtctgcaa aatcccaatc gataatcttt gtttttttcg cttctggaaa
     4081 atatttttat agagtttatt tctttcaacc tagaaaagaa aattgatggc acatataggc
     4141 ttccatgggc cttgcttttt tagagtcttt tgctgcttct agctcctcaa gtaaatctgc
     4201 taaactcatc taaaactcct catgcccgac caaatggtgc tgaaaggcat agacagccgc
     4261 ctgggtacga tcgctaactt caagtttggc aagaatgttg gacacgtggg tcttgaccgt
     4321 cttgagagag ataaagaggt catctgcgat gcgctgattt tcgtagccct tggcgatgag
     4381 ttggagaaca tctcgctcac gcgcagtcaa ttcttcatga agttccatat gattgcggtg
     4441 gtattcaacc ttcttgctaa cctcttgctc aatggccagc tcgccagcag ctaccttact
     4501 gacggcatga agcaattcat ctgcactaga agtcttgagc atatagcctt tggcaccagc
     4561 atctaagact ggcatgattt tttcattgtc caaataagag gtcacaatca aaatcttggc
     4621 ttcaggccat tctttaagga ttgctaaggt cgcatcaatc ccattcatct caggcatgac
     4681 aatatccatg acaatgacat ctggacgcag ttccaaggcc aagtcaatcc cttgagaccc
     4741 gttggacgcc tcacccacaa cttctacatc gtcttggagg tcaaagtagc ttttcaagcc
     4801 caatcggacc atttcatggt catctactag taaaattttc atctttactc ctttatcatt
     4861 ccttatctaa caggggaata cggatatcaa ccgccagccc ttgcttggga gctgtcaaga
     4921 gttgaactgt tccagccata tcttcaaccc gctccttgat atttcgcagt ccataactca
     4981 agtcgtctaa gctccctaac tggaaaccaa tcccattgtc caccaccttc agttgcaatt
     5041 caacatctgt ctgatagagg tagacatcta ggcaagatgc ctgggcatgg cggagggtat
     5101 tgctaatcaa ctcttgcagg atacggaaga tatgctcctc gattttctta ggcaatttcg
     5161 tcatattctg cttgagacta accctaagat cactcttgtc ctcaagctct tttaagagaa
     5221 tttgaatccc ttctatcaag ctcttctgct ccagttcaac tggtcgcaaa tgcaagagca
     5281 aaacccgcaa atccttctgg gcagtttcta aaatagctgt gacactctgc aactgggttt
     5341 gcatcttttc tctatccaat ttcaaagcct gctgactgat acccgataaa atcatgtggg
     5401 ccgcaaacaa ctcctgactg actgtatcgt gcaaatcccg agcaattcgc ttccgttcct
     5461 tctcgatgat ttcctcttcc tgagcaaggc tctgattttc agctttttga agagcctctg
     5521 tcaaaaggtt aagtttacct gataaggact tgaaactggc atccaaatct ggatctacaa
     5581 cctgaactac ttcttgccct gccaataaac gcttgagatt agcctgcatt tttcttagag
     5641 aaagctcttc gatccctcgc caaaacaggg ctaagagaca ggtcatggac atgctgaaaa
     5701 ccaacaataa aaagacaaat ttttctgttt tttcgacatc gtgcaaaaag atagaccagt
     5761 caaaatcaag tatttccagc aagctatggg agaaaaaaaa gacaaacagg aaggaggtga
     5821 gagcgataat gacataggct tgttttttca tcctctaacc acctccacat caccaatcat
     5881 agtggtcaag aaaatcttga cactcttgtt actcttgaga tagtcttttg tttcttgatg
     5941 atagtgttca ttgcggaggg ctcgcttggg ctggttgaaa aaagtcaaat ccccatagag
     6001 acagttaacg ctgagactga cttccacatc tacaggtacg atgattttgg tcgttcctac
     6061 catctttctg aggataatga cattgtcatg attggttaag atgaccctct ccagatgaat
     6121 agtgtccttg cccatgaagc gaaagagatt gatatcatcg aattggcaag tctggtagct
     6181 tgaaaaatga tgaagatttc caaaccaacg atttttctcc ttcttaaccg tcacgacctc
     6241 ttcaaaaacc aaattggtct gctctttttc ctggttcatc atcgggtaaa gaagaaagag
     6301 gctatagata accgcaacaa aaatagctag aatcacaaaa ggattgagca taacgatgaa
     6361 aaagaagaga atggttgccg ctactaaaag aagattattt ccctctttac cagtgtagta
     6421 gcgaatcaaa agcaaaaaga ggaatagtat cagcagaaaa cgcgaaaaat gctctgatac
     6481 catcaaaatc agagctcctg tcagaagaca ggcttcgata aataaaaaga ttttaaattt
     6541 tctcataggt tcatcctctc ccttctattt tatcacaatt caaaaaagtc acctcagtct
     6601 gaggatggaa aaaaaggcgc tggttacgcc tttttcatct gatcatttgc ttcttttaat
     6661 tttccataaa gaagatagtc tactttttgt agatctgcta tggtggcaca gttaagggaa
     6721 cacataatca agcgtagatc tgctttccag ccttggacaa tgccaatcac ttcttcaact
     6781 gtgtaggttt caaccaattc cagaacggtt cgtgacaatc ccacagcctt agcaccaaaa
     6841 accaagcact taatcatatc cagcggattc cgaacccctc cactaaccaa gagttcgacc
     6901 ttatctttcc attcttgggc attgagaagg gcctgcatgg tagactgacc ccattgattg
     6961 aggtaatcac gctggccact acgacggttt tcgatatagg caaagctggt gccaccacga
     7021 cccgataggt ccactgtacg aacaccgaat tcataggctc tttcgattgt cttggcatcc
     7081 attccaaagc ccacttcctt gaggacaata ggaacgggaa tttgcttgct ataatctgct
     7141 agatgcgatt gccagcttct aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg
     7201 acattgacat gcacttgcaa tagaacagga ttcatctctt ctacagtctg aagtcctaac
     7261 tcgacaggct tgtccaatcc aatattggtt ccaaggagga gattgggatg actagacttg
     7321 acagaaaaag aatcatccgt tggatttttg agggctgcgc tataagaacc cgttacaaat
     7381 aaaataccac aggattccgc cacctgagcc agcttttgat tgatttctct tcccttatta
     7441 cttccaccag tcatggcatt gatataaaaa ggaaagtccc actttcgacc agcaaactct
     7501 gtcgaaagat cgatttcatc cagattgtaa agaggcaagg aagaatgaat cagctccacc
     7561 tcatcaaagc tattatagga acttttctgc tcaagggcat agaggatatg ctcgtcctta
     7621 cgatttgtcg tcatgtccta tcctttcttg atataagagc tcaatcccca gatcggccca
     7681 acgatttttt aaggttttgg ttgattgcgc atcaaaactc agggcgatgc cacagtcacc
     7741 accaccagca ccactactct tggcaacggc ctgcaaatct tgactggctt ctttcaactg
     7801 tctaagcaaa ggcgtgtaaa tatctgtact caagccttct aaaagcttgc tggctacttc
     7861 tacttgctcg ataatctttt ctgatttccc ctgttccaag gcttcgacca aagaaaccac
     7921 cgtttctttt gaggaactta aaaaattttg attgatattt tgcttgattt gctggaccat
     7981 gtgactcgat acagccactt ccttggtcca tcccactaag aaatcacatt ctaaagttgg
     8041 tttcacttgt gaaattgaaa agccccaatc acgctccaga actgtcgcca agttttcttc
     8101 ttctaaccaa gcagccacct tctggcgatc aaatgactgg tagagaacca aatcctctgc
     8161 cacaatacag gcaaggtcgc ccatggaacc attgtctcct cgcttgagca agacagcgct
     8221 agtcagcttg aacaagagct cctgatcaac agaaacatca tacagagcca gtaaagcctt
     8281 gacaaccaag acaacgacgc tgccactaga acctagacca aactttttcc cttctcgttc
     8341 cattttgcca cagatttcta gagaaaaagg tcttaaattc tgaccacgaa cagcgaggaa
     8401 gtctcccatc aaagcaatcg tttcttgaat caagctgtag tcaggattag gccttaagtc
     8461 cactgcgaaa tcaaacatat ctgaatagat acggtagctg tcagaaaaag caatctcagc
     8521 cctcatatag atgggaatat cctttatcaa agctaactgc cctggctcta aaatagcata
     8581 ttcacctgcc caatagagtt ttccgcaagt tttaacagca atcatcttga ctcaaatcct
     8641 ttgtttttga cacaatcaag cgataacgat gaccgaaaat ttctgataaa tgctccaagt
     8701 ctttctcctg acagaagacc ttaacattgg gaccagcatc catggtaaag tagcaggcct
     8761 ctcctttctc acgaagctgg cgaacaaagt ccatagcctc ataagaggca tccgtcagat
     8821 aagaaaaggc tggactagca gtctttgtcg tagcatgcat agccagggca tttttctccg
     8881 ttaattctcc aatcttggca aaatcatttt ccttgagata aatcagcata tcctgatagt
     8941 ccttctcaga ctgacgaacc cagtcgtcaa aagtcgtcga ggtttccaca caaagtttca
     9001 tcccgtcacg gctagagatt ggttttttct tgtcctctag caccaacata atcatagcta
     9061 gtttcaagtc tgtctctaca gggtaaattt ctccactatc cttatcccag gctcctagtg
     9121 gtccataaaa actccgagaa gaagaacctg aggcaaattt ggcttcctgt gccaactgac
     9181 ttctatccaa tccaagcttg aaataagcat tacaagcctt gaccagggcg gacaaaccac
     9241 tagaacttga ggacagaccc gctgccgtag gcatattgtt ttgagtatcg atacggacaa
     9301 agccctcacc agctggacga taacggtcaa taatcttact catcttggca tgctcgacct
     9361 cattttgtag ctgaccattg atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg
     9421 acaaggtcgt ctctgtatac atattttcca aagttagaga aatactgcta gtagcaggca
     9481 ccatctcttt ttcttttttc tttccccaat atttgataat agcaatattt gcgtaggaac
     9541 gtactgttac aggctctcta tccatgtctg aacagctcct ttctcttcta atctttctgc
     9601 tagttcttgt gcgtgtgtca aattggttac caaggctatg atacaacctc ctagcccacc
     9661 accgctcatc ttggcaccca gagcaccatg gctaagagtc gtttcaacca aaaagtctgc
     9721 ctcagggcta ctgactccaa tttcttttaa atgtaaatgc gcttgactga ggatttgtcc
     9781 cagtccttca gcatcttttt gtgaaatcgc aacttctgct tgctgggtta attctcccaa
     9841 ggcatgcaaa aacggtaggg catccttgcc cttattttga accacttgga tggcttcacg
     9901 agtatgacca taaacacccg tatcggcaat caccaaatag gcggataaat ccatctcaag
     9961 ttctgtaaat cctacgttct tgataaagcg aataggttgg tcactaagac aggtcttagc
    10021 atccaaacca ctaggattca tatgggcaat catttcagct cgattgacca agatttctag
    10081 tacatcatga ggcagatcag cctgatagta gtcaaatact gcacgaatgg ccgctatgct
    10141 gatagccgct gacgaaccca tcccccgttt ctcagggata gccgagtcaa tctcacaacg
    10201 aatgcaggct tctgtgatat tcaaatactc cagtgaggca taaaccgcca tggacaaggt
    10261 atcctcctca taaaggcgcc aaggactctc tgcaggaact accttacagg tcacctccac
    10321 ctccaaaaga ggcagggaaa tggcaggata accgtaaacg accgcatgtt cccctattaa
    10381 aattatctta ctatgtgcct gaccgacacc aacttttttt gtcatttttt ccttttacta
    10441 gacgaaaaaa cgtcttattt ttcatacaag tattaattct ttcctatcta ttttattata
    10501 ttttcacaaa aaaagcgatt gtttccattc acaatcgctt ctttcattat tgaacccatt
    10561 cgccattata gttgacagaa tagccatcta cggtcgtatt cactgccaag gcacctgagc
    10621 tataagcgta gtaccatctg ccattgacct ggaaccaacc tgtcttcatg tctccattac
    10681 ctgcatttag gtagtaccaa gttgaaccat cttgatacca accagttgcc atagctcctg
    10741 atgaacggag atagtaccat ttgttcccaa ggttttgcca acctgttttc atatcgccat
    10801 ttggctggtc taaataatac caagtggtac cttcctgata ccagccagtg gccattgctc
    10861 ctgatgaacg gaggtagtac cagttattac ctagatattg ccatcctgtt cgcataatgc
    10921 cagtttctgg gtctaggtag taccatggag caggtttgct tctatcaatg tcaatagtaa
    10981 catgaaaatc ttgagtccaa cccttagcaa cttcactaat tggtagtgga ttgtaagaat
    11041 catcgccaaa atttcctagc ttatttaaat aataccaatt gccgtcttca taaatccagc
    11101 ctgtctttac agcataatta ttatcaaaat aataagttct cttttcagca gatagaggat
    11161 attgttcacc gtatactttt cctgtatttt cagacgtttt tgcctctagt acttgtttat
    11221 ctgtttgttc tagcaaagca ccatcttgtc caaaatagta ccacttctcc tgaaggccaa
    11281 tttcgaagac tggacgatta tcaaataaat catcacgata acctgttcca tgaatttcta
    11341 agtattgcca accgacaacc atctctccaa tatcaccaaa atagtaagtt ttaccgtcta
    11401 ttttatgcca atagattgct ttatggtcat cttttacata atattttcta ttatccttat
    11461 caacaaatcg gccacctgtg gtattcgcaa ataccgtatt tgtcgctagc aaaccaaaga
    11521 aaaagacggc taatgcgatt ggcatcattt ttttcaaaag cttcatctat atatcctcct
    11581 aaaacgattg tagtaaaaac tagattgtat gtcaatatat aaaaactcct caaaaaagca
    11641 gttacacaac tgcaactgct tttcta
//
LOCUS       DAVKZB010000056        11557 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKZB010000056 DAVKZB010000000
VERSION     DAVKZB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.056"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..1984
                     /gene="pspC"
                     /locus_tag="VXA80_001317"
     CDS             <1..1984
                     /gene="pspC"
                     /locus_tag="VXA80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV0353805.1"
                     /translation="YSIRKFSIGVASVAVASLVMGSVVHATEKEGTTQVATSSNMAKT
                     EHKKAAEQFDEYINKMIQLDRRKDIQNRTLIIKLSAIKTEYLRELNVLEYKSKAELPS
                     KIKAKLDAAFDQFKKDTLPTEPEKKVAEAEKKVEEAEKKAKAQKEEDRRNYPTNTYKT
                     LELEIAEFDVEVKKAELELVKEEAKKPLNEGTIKQAKEKVESKKAEATRLENIKTDRK
                     KAEEEAKRKADAKEQDESKRRTKRAVPGELATPDKKENDAKSSDSSVGEETLPSPSLK
                     SGKKVAEAEKKVEEAEKKAKAQKEEDRRNYPTNTYKTLELEIAESDVEVKKAELELVK
                     EEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEAKRKAAEEDKVKEKPAE
                     QPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPK
                     TEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNN
                     GSWYYLNANGSMATGWLQNNGSWYYLNANGAMATGWLQNNGSWYYLNANGSMATGWLQ
                     NNGSWYYLNANGSMVTGWLQYNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQ
                     WFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(2214..3194)
                     /gene="dusB"
                     /locus_tag="VXA80_001318"
     CDS             complement(2214..3194)
                     /gene="dusB"
                     /locus_tag="VXA80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV0353806.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(3181..4053)
                     /gene="hslO"
                     /locus_tag="VXA80_001319"
     CDS             complement(3181..4053)
                     /gene="hslO"
                     /locus_tag="VXA80_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV0353807.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            4218..5645
                     /locus_tag="VXA80_001320"
     CDS             4218..5645
                     /locus_tag="VXA80_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0353808.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            5804..7312
                     /gene="glpK"
                     /locus_tag="VXA80_001321"
     CDS             5804..7312
                     /gene="glpK"
                     /locus_tag="VXA80_001321"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV0353809.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            7354..9180
                     /gene="glpO"
                     /locus_tag="VXA80_001322"
     CDS             7354..9180
                     /gene="glpO"
                     /locus_tag="VXA80_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV0353810.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            9250..9954
                     /locus_tag="VXA80_001323"
     CDS             9250..9954
                     /locus_tag="VXA80_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0353811.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            10251..10454
                     /locus_tag="VXA80_001324"
     CDS             10251..10454
                     /locus_tag="VXA80_001324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353812.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(10541..11128)
                     /locus_tag="VXA80_001325"
     CDS             complement(10541..11128)
                     /locus_tag="VXA80_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353813.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWMFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         3557 a         2265 c         2609 g         3126 t
ORIGIN      
        1 ttattcaatt cgtaaattta gtattggagt agctagtgta gctgttgcca gtcttgttat
       61 gggaagtgtg gttcatgcga cagagaagga gggaactacc caagtagcca cttcttctaa
      121 tatggcaaag acagaacata agaaagctgc tgaacaattc gatgaatata taaacaaaat
      181 gatccaatta gatagaagaa aagatatcca aaatcgcacc ttaatcataa agttgagcgc
      241 aattaaaacg gagtatttgc gtgaattaaa tgttttagaa tataagtcga aagctgagtt
      301 gccgtcaaaa ataaaagcaa agttagacgc agcttttgat cagtttaaaa aagatacatt
      361 accaacagaa ccagaaaaaa aagtagcaga agctgagaag aaggttgaag aagctgagaa
      421 aaaagccaag gctcaaaaag aagaagatcg ccgtaactac ccaaccaata cttacaaaac
      481 gcttgaactt gaaattgctg agttcgatgt ggaagttaaa aaagcggagc ttgaactagt
      541 aaaagaggaa gctaagaaac ctttaaacga gggcacaatt aagcaagcaa aagagaaagt
      601 tgagagtaaa aaagctgagg ctacaaggtt agaaaacatc aagacagatc gtaaaaaagc
      661 agaagaagaa gctaaacgaa aagcagatgc taaagagcaa gatgaatcaa agaggcggac
      721 aaaacgagca gttcctggag agctagcaac acctgataaa aaagaaaatg atgcgaagtc
      781 ttcagattct agcgtaggtg aagaaactct tccaagccca tccctgaaat caggaaaaaa
      841 ggtagcagaa gctgagaaga aggttgaaga agctgagaaa aaagccaagg ctcaaaaaga
      901 agaagatcgc cgtaactacc caaccaatac ttacaaaacg cttgaacttg aaattgctga
      961 gtccgatgtg gaagttaaaa aagcggagct tgaactagta aaagaggaag ctaagggatc
     1021 tcgaaacgag gaaaaagtta agcaagcaaa agcggaagtt gagagtaaaa aagctgaggc
     1081 tacaaggtta gaaaaaatca agacagatcg taaaaaagca gaagaagcta aacgaaaagc
     1141 agcagaagaa gataaagtta aagaaaaacc agctgaacaa ccacaaccag cgccggctcc
     1201 tcaaccagaa aaaccagctc cagctccaaa accagagaat ccagctgaac aaccaaaagc
     1261 agaaaaacca gctgatcaac aagctgaaga agactatgct cgtagatcag aagaagaata
     1321 taatcgcttg actcaacagc aaccgccaaa aactgaaaaa ccagcacaac catctactcc
     1381 aaaaacaggc tggaaacaag aaaacggtat gtggtacttc tacaatactg atggttcaat
     1441 ggcgacagga tggctccaaa acaatggctc atggtactac ctcaacagca atggcgctat
     1501 ggcgacagga tggctccaaa acaatggttc atggtactat ctaaacgcta atggttcaat
     1561 ggcaacagga tggctccaaa acaatggttc atggtactac ctcaacgcta atggcgctat
     1621 ggcgacagga tggctccaaa acaatggttc atggtactat ctaaacgcta atggttcaat
     1681 ggcaacagga tggctccaaa acaatggctc atggtactac ctaaacgcta atggttcaat
     1741 ggtgacagga tggctccaat acaatggttc atggtactac ctaaacgcta acggttcaat
     1801 ggcaacagat tgggtgaaag atggagatac ctggtactat cttgaagcat caggtgctat
     1861 gaaagcaagc caatggttca aagtatcaga taaatggtac tatgtcaatg gctcaggtgc
     1921 ccttgcagtc aacacaactg tagatagcta tagagtcaat gccaatggtg aatgggtaaa
     1981 ctaaacctaa tataactagt taatactgac ttcctgtaag aactctttaa agtattccct
     2041 acaaatacca tatcctttca gtagataata tacccttgta ggaagtttag attaaaaaat
     2101 aactctgtaa tctctagctg gatttatagc gctagagact acggagtttt tttgatgagg
     2161 aaagaatggc ggcattcaag aggctcttta agagagttac gggttttaaa ctattaagcc
     2221 ttctccaatt gcaagagggc ttcaatctct gccagggtgc tagcttgcga aatggctcca
     2281 cggagtttgg cagcgccaga tgttccacgg aggtagtgag gagcgaggcc gcggaattca
     2341 cgaactgcga cgttttctcc tttgaggtta atcaatcgtt tcaagtgttc gtaggcgatc
     2401 ttcatcttgt cttcaaaggt caaatcaggt aggatttctc ctgtttcaaa gtaatggttg
     2461 atttggttga agaggtaagg atttcccatg gcagctcggc caatcatgac tgcgtcagca
     2521 ccaacttctt cgatgcgttg cttggcttct tggacagtac ggatatcacc gttggcgatg
     2581 aatggaatct tggttagagc ttgggcaacc ttgtaaaggg tctcaaggtc tgcgtggcca
     2641 gtatacattt gttcacgggt acggccatgc atggcgaggg cagaaacacc tgcagcttca
     2701 gcagcgaggg cattttctac tgcaagagat gggtccgccc agccagtacg cattttgaca
     2761 gtaagtggga tatcaaggac agactggacc ttgttgatga tggagtaaat cttgtcagga
     2821 tccttgagcc acatagctcc agcttcgttc ttcacgattt tgttgacagg gcagcccatg
     2881 ttgatatcga cgatatcggt cttggtgttt tcttggatga attctgctgc gcgtgctagg
     2941 ctgtcttcat cgctaccaaa aagttggata gagacagggt tttcgccctc atcgatatga
     3001 agcatgtgca gggttttttc gttgttgtat tggattccct tgtcagagac catttccatt
     3061 acaacgagtc cagctccgag ctcttttgcg atggtacgaa aggctgagtt ggtcacgcca
     3121 gccataggcg ctaaaacggt acgattggga atctcaacat tgccaatcat aaaaggtgta
     3181 ttaagatttg tcacgaatga gttcctccag gtccttttca tcaaagttgt aagtagtttg
     3241 gcagaattga caagtgattt ctgccccgtg gtcttcctct ttcatttcct gtaagtctga
     3301 gcttggaagg ctggcaagag cgttcataaa gcgttcatgg ctacagtcac attggaaacg
     3361 gatttcttct tcagaaagac gcttgtaggc ttcgtccccg tagatagcct tgaggagggc
     3421 ttcgatatgg tcgtcgcttt cgagaagagt agagatagct ggcatttctt ggatgcgttt
     3481 ttcaaagcga gcaatctctt ctttcttggc tcctggcaag acttgaacta ggaaaccacc
     3541 tgcaaccttg accttgtctt cctcgtccaa aaggacattg aggccgaccg ctgaaggcgt
     3601 ttgttggctt tcagtaaggt aaaaggcaag gtcttcaccg atttctccag agatgagggg
     3661 agttatagag ttgtaaggat ttccagtacc gtagtctgtg ataacgagga attgaccatt
     3721 tccaacaaaa ggtccgacta ggacttcacc agtcgcagtc tttttgatgt caacaccagg
     3781 attttgaaca tagcctttga cgttcccctt ggtatcagcg acggtgataa tagcacctag
     3841 agagctagat cccaacacct taactgtaag tttggtattt cctttttcat tggctgcgag
     3901 aatctggcta gcgataagag ttcgaccaag cgctacagtt gagctagctt gggtttgatg
     3961 tttttcttga gcagtgcgga cggtttcagt gctatcaagg acaaaagcac gaaaggctcc
     4021 gctttctgat atagttttaa taattttatc catagctact attttagcat aaaaatgccc
     4081 aaagggggag ccgtgtgttt actgattttc aggataatgg accaggaaat cagcatgaaa
     4141 ataaaaagag aaacagatta ttttagcatt tgtcagattt atgctatgct taaggtagaa
     4201 aatgaaaggg ataacaaatg tatttaggag atttgatgga gaaagccgag tgtggtcaat
     4261 tttcaatcct ttcctttcta ttacaagagt ctcagacgac cgtcaaggct gtaatggaag
     4321 aaacaggatt ttcaaaagca accctaacca aatatgtcac cctgctcaat gacaaggctt
     4381 tggatagtgg cttagagctg gctattcact cagaagatga aaatctgcgt ctgtctatcg
     4441 gtgcagctac caaggggaga gatattcgga gcttgttttt ggagagtgct gttaaatacc
     4501 agattttggt ttatcttctc taccaccaac agtttttagc ccatcagctg gctcaagaat
     4561 tggtgattag cgaggctacg cttggtcgtc acttggctgg tttaaatcag attttgtcag
     4621 aatttgattt atccatccaa aatggacgtt ggcgaggtcc agagcatcag attcgctatt
     4681 tctatttctg tcttttccga aaggtctggt cgagtcagga atgggaaggt cacatgcaga
     4741 aaccagagag aaaacaggag attgccaatt tagaggaaat ctgcggtgca agtttgtctg
     4801 tggggcagaa attggacttg gttctctggg ctcacatcag tcaacaacgt cttcgggtca
     4861 atgcttgtca gtttcaagtc atagaagaga aaatgcgagg gtattttgac aatatcttct
     4921 atcttcgttt gctgagaaag gttccgtcct tttttgctgg gcaacatatt ccactaggag
     4981 ttgaggatgg tgagatgatg atattcttct cttttctcct atctcatcgc attcttcctc
     5041 ttcatactat ggaatatatt cttggttttg gagggcagtt ggcagattta ctgacgcaat
     5101 tgattcaaga aatgaagaag gaggaactat tgggggatta tacagaggac catgtcacct
     5161 atgaactcag tcagctttgt gctcaagtct atctctataa gggctatatt ttacaggatc
     5221 gctacaagta ccagttagag aatcgtcatc catatttact gatggaacat gattttaaag
     5281 agacagcaga ggagattttt catgctctac ctgcttttca acaggggaca gatttagata
     5341 agaagattct ctgggaatgg ctccagttaa tcgaatatat ggctgaaaac ggtggccagc
     5401 atatgcggat tggtctggat ttgacatctg gttttcttgt cttttcaagg atggcagcca
     5461 ttttgaaacg gtatttggaa tacaatcgtt ttattaccat tgaagcttat gaccctagtc
     5521 ggcattatga tttgctggtt accaataacc cgattcataa gaaggaacag accccagtct
     5581 attatttaaa aaatgacttg gatatggaag atttggcagg gattcgtcag ttattattca
     5641 cttaaaaggc ttggttaatc caggtctttt ttgtgaaatt cacacaatct cctcacattt
     5701 ttttaaaaat taaaaaaagt tgataaacaa gaaagcgctt tattttgtat actagtaagt
     5761 gtaaagagga aacacctcaa gatctttatc aggaggacag tacatgtcac aagaaaaata
     5821 catcatggcc attgaccagg gaactacaag ttctcgtgcc atcattttca acaaaaaagg
     5881 ggaaaaggtt agctcgagtc aaaaagagtt tacccagatt ttccctcagg caggttgggt
     5941 tgagcacaat gccaatgaaa tttggaactc tgttcagtca gttattgcgg gtgctttcat
     6001 cgaaagtggt gtcaagccaa atcaaatcga ggcaatcggg attaccaacc aacgtgaaac
     6061 aacggttgtc tgggataaga aaacaggact tcctatctac aatgctatcg tttggcagtc
     6121 acgccagaca gcacctttgg ctgagcaact aaaaagccaa ggttatgtgg aaaaattcca
     6181 tgaaaagact ggtttgatta ttgatgctta cttctctgct accaaggttc gttggatttt
     6241 ggatcatgta gaaggcgctc aagagcgagc agaaaaaggg gaattgctct ttggtactat
     6301 cgatacttgg ttggtttgga aattgactga cggtgcggct cacgtgactg actactcaaa
     6361 tgcagctcgt accatgcttt ataacattaa agaactcaaa tgggatgatg agattttgga
     6421 aatccttaac attccgaagg ctatacttcc agaagttcgt tctaactccg aaatctacgg
     6481 caaaacagct ccattccatt tctacggtgg agaggtgcca atctcaggta tggctgggga
     6541 ccaacaagca gccctctttg gacagttggc ttttgagcca ggtatggtta agaatactta
     6601 tggaacaggc tctttcatca tcatgaatac tggggaagag atgcagttgt ctgaaaacaa
     6661 cctcttgaca accattggtt acggaatcaa cggtaaggtt tattatgcct tggaaggttc
     6721 tatcttcatc gcaggaagtg ctattcagtg gcttcgtgac ggtcttcgca tggttgaaaa
     6781 ttcaccagaa tctgaaaaat acgctcgtga ttctcacaac aacgatgaag tttatgtcgt
     6841 tccagccttt acaggtctag gcgctccata ctggaaccaa aatgctcgtg gttccgtctt
     6901 tggtttgact cgtggaacaa gcaaagaaga ctttatcaag gcgactttgc aatctattgc
     6961 ttatcaagtg cgtgacatca tcgacaccat gcaagtggat actcagactg ccattcaagt
     7021 attgaaggtg gatggtggtg cagccatgaa caacttcctc atgcagttcc aggcggatat
     7081 tttaggcatt gatattgcac gtgctaaaaa cttggaaaca acagctctag gagcggcctt
     7141 cctagcaggt ttatcagtag ggtactggaa agacttggac gagttgaaac tcttgaacga
     7201 gacaggagaa ctctttgagc catctatgaa tgaatctcgc aaggaacaac tctacaaggg
     7261 ctggaagaag gctgtgaaag caactcaagt ctttgcggaa gtagacgact aatactggca
     7321 gaataaagcg atttatttag aaagtgtgta aatatggaat tttcaaagaa aacacgtgaa
     7381 ttgtcaatta aaaaaatgca ggaacgcacc ctggacctct tgattatcgg tggaggaatc
     7441 acaggagctg gtgtagcctt gcaggcggca gctagcggtc ttgagactgg tttgattgaa
     7501 atgcaagact ttgcagaagg aacatctagt cgttcaacaa aattggttca cggaggtctt
     7561 cgctacctca aacaattcga cgtagaagtg gtctcagata cggtttctga acgtgcagtg
     7621 gttcaacaaa tcgctccaca cattccaaaa ccagatccaa tgctcttacc agtttacgat
     7681 gaagatggag caacctttag cctcttccgt cttaaagtag ccatggactt gtacgacctt
     7741 ttagcaggtg tgagcaacac accaactgcg aacaaggttt tgagcaagaa agaagtcttg
     7801 gaacgccagc caaacttgaa gaaggaaggc ttggtaggag gtggagtgta tcttgacttc
     7861 cgtaacaacg atgcgcgtct cgtgattgaa aacatcaaac gtgccaacca agacggtgcc
     7921 ctcattgcca accacgtgaa ggcagaaggc ttcctctttg acgaaagtgg caagattaca
     7981 ggtgttgtag ctcgtgatct cttgacagac caagtatttg aaatcaaggc ccgtctggtt
     8041 attaatacaa caggtccttg gagtgataaa gtacgtaatt tgtctaataa gggaacgcaa
     8101 ttctcacaaa tgcgcccaac taagggagtt cacttggtag tggattcaag taaaatcaag
     8161 gtttcacagc cagtttactt cgacacaggt ttgggtgacg gtcgtatggt ctttgttctc
     8221 ccacgtgaaa acaagactta ctttggtaca actgatacag actacacagg tgatttggag
     8281 catccaaaag taactcaaga agatgtagat tatctacttg gcattgtcaa caaccgcttc
     8341 ccagaatcca acatcaccat tgatgatatc gaaagcagct gggcaggtct tcgtccattg
     8401 attgcaggga acagtgcctc tgactataat ggtggaaata acggtaccat cagtgatgaa
     8461 agctttgaca acttgattgc gactgttgaa tcttatctct ccaaagaaaa aacacgtgaa
     8521 gatgttgagt ctgctgtcag caagcttgaa agtagcacat ctgagaaaca tttggatcca
     8581 tctgcagttt ctcgtgggtc tagcttggac cgtgatgaca atggtctctt gactcttgct
     8641 ggtggtaaaa tcacagacta ccgtaagatg gctgaaggag ctatggagcg cgtggttgac
     8701 atcctcaaag cagaatttga ccgtagcttt aaattgatca attctaaaac ttaccctgtt
     8761 tcaggtggag aattgaaccc agcaaatgtg gattcagaaa tcgaagcctt tgcgcaactt
     8821 ggagtttcac gtggtttgga tagcaaggaa gctcattacc tagcaaatct ttacggttca
     8881 aatgcaccga aggtctttgc acttgctcac agcttggaac aagcgccagg tcttagcttg
     8941 gcagacacct tgtcccttca ctatgcaatg cgcaatgagt tgactcttag cccagttgac
     9001 ttccttcttc gtcgtaccaa tcacatgctc tttatgcgtg atagcttgga tagtatcgtt
     9061 gagccaattt tggatgaaat gggacgattc tatgactgga cagaagaaga aaaagcaact
     9121 taccgtgctg atgtcgaagc agctctcgct aacaacgatt tagcagaatt aaaaaattaa
     9181 gaaaaaataa aagaggtgga gggcagcatt ccttgtcgcc cgtcccttct ttttaatgga
     9241 gacagaaaga tgatgaatga attatttgga gaatttctag ggactttaat cctgattctt
     9301 ctaggaaatg gtgttgttgc aggtgtggtt cttcctaaaa ccaagagcaa tagctcaggt
     9361 tggattgtga ttactatggg ttgggggatt gcagttgcgg ttgcagtctt tgtatctggc
     9421 aagctcagtc cagcttattt aaacccagct gtgaccatcg gtgtggcctt aaaaggtggt
     9481 ttgccttggg cttccgtttt gccttatatc ttagcccagt tcgcaggggc catgctgggt
     9541 cagattttgg tttggttgca attcaaacct cactatgagg cagaagaaaa tgcaggcaat
     9601 atcctggcaa ccttcagtac tggaccagcc atcaaggata ctgtatcaaa cttgattagc
     9661 gaaatccttg gaacctttgt tttggtgttg acaatctttg ctttgggtct ttacgatttt
     9721 caggcaggta tcggaacctt tgcagtggga actttgattg tcggtatcgg tctatcacta
     9781 ggtgggacaa caggttatgc cttgaaccca gctcgtgacc ttggacctcg tatcatgcac
     9841 agcatcttgc caattccaaa caagggagac ggagactggt cttacgcttg gattcctgtt
     9901 gtaggccctg ttatcggagc agccttggca gtgcttgtat tctcactttt ctagtttata
     9961 ctcttcgaaa atcaaattca aaccacgtca gcgtcgcctt accgtactca agtacagctt
    10021 gcggctagct tcctagtttg ctctttgatt ttcattgagt attagaaaac aattatgttg
    10081 atagagcttg ggcaagagcc caatttcagc aaaaaatgaa gtaaatcttc tcataataaa
    10141 acgcatcata tcaagcacga aaattccacg aggtcaacta cagtcagaaa gctgaacaac
    10201 aagccaaaac gcccaaaaaa ggcggcaaaa agcaagcacc tgcaagcaac gtgccgaaat
    10261 ggtcaaatcc tgattatgtc aacgaattag acccaaaaat cgttgatatg ctagtagaat
    10321 ttcacaagtc acaaggcact ttggaaactc ccgaggcgca agcagaaatc gcccaaaaac
    10381 gtgaagaaat cgagcaaagg agagctgagc ttgaggataa aaaacaagag cttttgaacc
    10441 gcttgaacaa atagagtttc gcaagtatta tgcttacaaa ttacttgagc aattaactaa
    10501 aatataaacc ctgcctttat atctaggcag ggtttatatt ttagaaattc acgtaggttg
    10561 ttacggtttt tacataccca gtatagtttg agtttctata gtattcagtg ataaacatcc
    10621 attttttttg agcaacatgg atataagtac ttgttatgta gtatggatat gggctttgtg
    10681 aatccaagta agactgataa gcttgtatac caaaatatgc tccaccaatt attgcacccc
    10741 atggaccccc caataaagca cctatcctac caatcatata actgattcca gcaccagtca
    10801 tgaagttagc gaatgtgtta gcttgtttat tcccatgtat tgtgttgacg taattccaaa
    10861 cattaggatc gtatgatcta aaagatatat ttaggtcgat ttcattcttt tgataagcca
    10921 tataaaatgc cccattgata tagacgccgt cagcacgtcg ttcaatagtg tctacacttc
    10981 catctggatt gacaacctca agaacttcat cgcttaaaat atttacttgc gtatctccga
    11041 accgcactga tgagccattc tcaaactgag cctcaccaga tacaacttta gagtttgccg
    11101 ataagctatc atcagcaaaa acaaacaagc gacggggaaa tgctagacat acagaaaaca
    11161 gacataacta gcaaacacat gcatttaaac atcttagaca taacggaaac tcctttgtat
    11221 ttttgatttt tttcaacttt tattatacaa taaaaccaaa taaaaagaaa gcggtaacaa
    11281 tatgcttaat gcgaaaattt tttatatatt tttatgtttg atcgttatcg aaactacagg
    11341 cttgttgttg ttgaaaagag gtctcgaaat gggttattta gacacagaag ctattatcct
    11401 cgcagttttt tcatttgctt tttacaacct atgttcattc gcttgggtct gctctacaat
    11461 aaaaaacaat aaaaaataaa tagacgtatt ttcaaaaaaa aaaaaaaaat gcatatttat
    11521 cttagcaaaa cgacgattta aatcgtcgtt tttttgt
//
LOCUS       DAVKZB010000057        11522 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKZB010000057 DAVKZB010000000
VERSION     DAVKZB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.057"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            109..762
                     /locus_tag="VXA80_001326"
     CDS             109..762
                     /locus_tag="VXA80_001326"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV0353814.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            772..1236
                     /locus_tag="VXA80_001327"
     CDS             772..1236
                     /locus_tag="VXA80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0353815.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1249..2517
                     /locus_tag="VXA80_001328"
     CDS             1249..2517
                     /locus_tag="VXA80_001328"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV0353816.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2531..2791
                     /locus_tag="VXA80_001329"
     CDS             2531..2791
                     /locus_tag="VXA80_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353817.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2802..4163
                     /gene="pdrM"
                     /locus_tag="VXA80_001330"
     CDS             2802..4163
                     /gene="pdrM"
                     /locus_tag="VXA80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV0353818.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4317..5285
                     /locus_tag="VXA80_001331"
     CDS             4317..5285
                     /locus_tag="VXA80_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV0353819.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5301..6293
                     /locus_tag="VXA80_001332"
     CDS             5301..6293
                     /locus_tag="VXA80_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV0353820.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSVRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6511..7554
                     /locus_tag="VXA80_001333"
     CDS             6511..7554
                     /locus_tag="VXA80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV0353821.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7618..9303
                     /gene="lpdA"
                     /locus_tag="VXA80_001334"
     CDS             7618..9303
                     /gene="lpdA"
                     /locus_tag="VXA80_001334"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV0353822.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVSGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9367..10356
                     /locus_tag="VXA80_001335"
     CDS             9367..10356
                     /locus_tag="VXA80_001335"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV0353823.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10429..11499)
                     /gene="xerS"
                     /locus_tag="VXA80_001336"
     CDS             complement(10429..11499)
                     /gene="xerS"
                     /locus_tag="VXA80_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV0353824.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3384 a         2179 c         2618 g         3341 t
ORIGIN      
        1 ttcaaatcgt atatagtcga tttattaaat ctttagataa attgtaaaaa gtggtttcca
       61 tagccacttt ttgctataat agaggcagta aaaatgaagg agtcagctat ggaacactcg
      121 tcttggcatg ctttgattaa ggcgcaatta cctgagggtt atttcgggaa aatcaatcag
      181 tttatggagc aggtctattc tcaggggatt atttatccac ccaaggaaaa ggtttttcag
      241 gctctcttga caacactgct tgaagaagtt aaggtggtaa ttctagggca agacccctat
      301 cacggaccag gtcaagcgca gggcttgagt ttttctgtac ctgactctat cccagctcca
      361 ccatccttgc aaaatatctt gaaagaattg tcagatgata tcggagttaa gaaatctcat
      421 gatttgacag cttgggctga gcaaggagtc ttgcttctta atgcttgttt gactgttcct
      481 gctggacagg ccaatggtca tgctggtcaa atctgggagc cttttactga tgctgtgatt
      541 caggtggtca atcatctaga tagaccagtc gtttttgtac tctggggagc ttatgcacgt
      601 aagaagaagg ccttagttac caatcctcat cacttgatta tcgaatcagc ccatccaagt
      661 cctttgtcgg tttatagagg attttggggt tccaagccct tttccaaggc caatgcattc
      721 ttaaaagaga caggacaaga gccaatcgat tggcttagat aaggagaaaa tatgcctcag
      781 ttagcgacga tttgctacat tgataatggg aaagaactgc tcatgctcca tcgtaataag
      841 aaacccaatg atgtccatga agggaaatgg attggtgtgg gtggtaagct agagagagga
      901 gagacgcccc aggaatgcgc ggcgcgtgaa atccttgaag aaacagggct caaagccaag
      961 ccagttctaa aaggtgtcat cacttttcct gaatttacac cagatttaga ctggtacacc
     1021 tatgttttta aggtgacgga gtttgagggc gacttgattg actgcaatga ggggacgcta
     1081 gaatgggttc cctatgacga ggttttgagc aagccgactt gggaaggtga ccacaccttt
     1141 gttgagtggc ttttagagga taaacccttc ttttcagcca agtttgttta tgatggggat
     1201 aaattgttgg atacccaagt tgatttctat gaataaagga gaaagcagat gctactaatc
     1261 aaaaatggtc gtgtaatgga tcctaagtct ggtttggatc aagtttgtga tgtcttagtt
     1321 caagatggga aaattatcaa aattgcgcct gagatcacgg aagaaggagc agaaacgatt
     1381 aatgctactg gtcttgtggt tgctcctggt ttggtcgata ttcatgtgca tttccgtgaa
     1441 cctggtcaaa cacataaaga agacattcat actggtgccc tagcagccgc tgcaggtggt
     1501 tttactactg tcgtcatgat ggctaatacc agtccaacca tttcagacgt ggagactttg
     1561 caagcagttc tccagtcagc tgccaaagag aagattaatg tcaagacagt tgcgaccatt
     1621 actaaaaact ttaatggtaa aaacttgact gactttaagg cgctcttaga agctggtgcg
     1681 gttggtttct ctgatgacgg tattccgctt gagagcagta agattgtcaa ggaagccatg
     1741 gaggaagcca aaaaactcaa tacctttatc agccttcatg aggaagatcc aggtttgaac
     1801 ggtgttcttg gctttaatga aaatattgct agagaacatt ttcatatctg cggtgctact
     1861 ggggtggctg agtacgctat gatggcgcgt gatgtcatga ttgcctatgc aactaaagcc
     1921 catgttcaca tccagcattt gtctaaggaa gaaagtgtta aagtagtgga gtttgctcag
     1981 gggttaggtg cagaagtcac agcagaagta gcgccacagc atttctctaa gacagaagca
     2041 cttcttttaa cacaaggtag caatgctaag atgaatccac cgcttcgttt ggaatcagac
     2101 cgtcgtgccg ttatcgaagg tctcaaatca ggtgtcatca cagttattgc gactgaccac
     2161 gcgcctcatc atgtagatga aaaaaatgtt gaggatatta ccaaagcgcc atctggtatg
     2221 actggcttag aaacatccct gtctctcagc ttgacctatt tagtagaagc tggtgagttg
     2281 agcttgatgg aattacttga aaaaatgaca tacaacccag ccaagcttta caactttgaa
     2341 gcaggttact tggctgagaa tggtccagca gatatcacta tttttgatgc caaggctgac
     2401 cgccttgtgg actcccattt tgcttccaaa gcagctaatt caccattcat cggtgaaacc
     2461 ttaaaagggc aggttaaata taccatctgt aagggacaaa tcgtctatca agcttgatag
     2521 gagttagaat atgaattatt ttcgaaaacg tagggagaga caagcgaaat ctaatagtgg
     2581 aatttactgt cccgcagcta atcgttccaa aattcaatat gaaactaaag aaaaagctga
     2641 ccatgcggtt caatatgata acggaggttt agttagaagc tattattgtc gtacatgtgc
     2701 gtgttggcat actacttcaa aatctaataa acctccgcta agtttgaaaa actatggtct
     2761 gaaactcttt ggtttagata aatcagaata gaaagagaag tatgtataag acaaagtgtt
     2821 tacgagagaa gttagtatta tttttaaaaa ttttcttccc aatcctgatc taccaatttg
     2881 ccaattattc tgcctctttt gttgatactg caatgacagg tcaatacaac actatggact
     2941 tggctggtgt atctatggca accagtatct ggaatccttt ctttacattt ctaacaggga
     3001 ttgtgtcagc cttggtgcct atcattggtc accatcttgg tcgaggcaaa aaggaagaag
     3061 ttgcgtctga tttttaccaa tttatttatt tggccttggg cctatctgtg gtcttgctgg
     3121 ggatggtact tttcttggca ccaacaatct tgaatcatat tgggttagaa gcagcagtag
     3181 cggcagtagc ggttcgctat ctttggtttt tatctatcgg gattatcccc ttgttgctct
     3241 ttagcgtcat tcgttccttg ctggattcgc tgggcttgac caaactgtcc atgtacctca
     3301 tgcttttgtt actccctctc aatagcggat ttaactatct cttgatttac ggtgcctttg
     3361 gtgttccaga actgggaggg gctggtgctg gtttaggaac atccttggcc tactgggtct
     3421 tgcttgggat ttctgttctg gttttattta aacaggagaa gctcaaagcc ttacaccttg
     3481 agaaacgaat tccacttaat atggataaaa ttaaggaagg agttcgttta ggtctgccta
     3541 ttgggggaac tgtcttcgcg gaagtggctg tcttttcagt ggttggcttg attatggcta
     3601 agttttcgtc cttgattata gctagtcacc agtcagctat gaacttttca agtcttatgt
     3661 acgcctttcc tatgagtatc tcatcggcta tggctattgt cgtttcctat gaagtgggag
     3721 ccaagcgatt tgatgatgcg aaaacctata ttggtctagg aagatggact gctctcattt
     3781 ttgcggcctt caccctaacc ttcctttaca tttttagggg aaatgtggcc agtctttatg
     3841 gtaacgaccc aaaatttatc gatttgacag cgcgtttttt aacttatagt cttttcttcc
     3901 agttagcaga tacctttgcg gcgccgcttc agggaatttt gcgggggtat aaggatacag
     3961 ttattccttt ttaccttggt ttgcttggtt attggggcgt agcaatccct gtggctacgc
     4021 tatttgattc cctaacagat tttggagctt attcttactg gatcggcttg attattagtt
     4081 tgattgtgag cggggcgctc taccgttggc gtttaactgt gattatgaag agatttgaat
     4141 ctttagcaaa atccaaacgc taaaaagttt gtgaaaaaat attcttgaca agaaaatccc
     4201 ttgttttcac ggggttttct tttttatttt gtgaaatttc ttttagaaaa actttgacag
     4261 tatttgccaa aagcggtaaa atagtaaggt aagaagaaag ttagaaaggc aagaagatgt
     4321 caactttaga taaaaatctt ttgctagaaa tgttccgtaa gatggaagaa atccgtcgca
     4381 tggacttaaa aattgcacaa ttagtaaaga aagggaaagt gccaggaatg acgcactttt
     4441 ctgttggtga agaggcagct aacgtggggg ctatgttagc tctcaatcca gatgatctga
     4501 ttacctcaaa ccaccgtgga cacgggcaag ctattgctaa agggattgac ctcaacggaa
     4561 tgatggctga aatccttggt aaatacactg gaacctgtaa agggaaaggt ggatctatgc
     4621 atatcgctga ccttgatgct gggaaccttg gtgccaatgg tatcgtaggt ggtggtatgg
     4681 ggatcgctgt cggtgcagcc ctcagtcagc aaatgcaaaa taccggtaaa atcgttgtct
     4741 gcttctttgg agatggtgcg accaatgaag gtgttttcca cgaagcagtg aacatggctt
     4801 ctatctggaa cctgccagtc attttctatt gcattaacaa cggttacggt atctctgcgg
     4861 atatcaagaa aatgaccaat atagaacata tccatcaacg tagcgccgct tatggaattc
     4921 ctggaatgtt catcgaagac ggtaacaatg tcatcgatgt ctatgaagga tttcagaaag
     4981 ctgtggacca tgttcgcagt ggcaatggtc cagtcttgat tgaaagtgta acttatcgct
     5041 ggctcggtca ctcatcatct gaccctggta aatatcgtac gcgtgaagaa gtggaattgt
     5101 ggaaacaaaa agatccaatc gaaaacctcc gcaattacct tattgaaaat aacattgcaa
     5161 gtgccgaaga attggaagaa atccaagcgc aagtaaagga agcagtagaa gcttctgtta
     5221 aatttgcaga ggaaagccca ttcccaccgc ttgaatcagc ctttgaagat atttacgcag
     5281 actaaggaga aagagataaa atggaaacaa aaacaatgtc cttccgtgac accattatcc
     5341 ttgctatgtc tgaggaaatg cgtcgcgatg aaaatgtgtt cttgatggga gaagacgtcg
     5401 gtgtcttcgg aggagacttc ggtacttctg ttggaatgct tgaagaattt ggtccagaac
     5461 gtgtccgtga ctgtccgatt tctgaagctg ccatctctgg agcagcagca ggagcagcca
     5521 tgacaggact tcgtccaatc gtcgatatga ccttcatgga cttctctgtt attgccatgg
     5581 acaatatcgt caaccaagct gctaaaacac gttacatgtt tggtggtaaa ggtcaggttc
     5641 caatgactgt tcgttgtgca gctggtaacg gagttggctc tgccgcccag cactcacaat
     5701 ctctagagtc ttggtttact cacattccag gacttaaggt ggtggcacct ggaacacctg
     5761 cggacatgaa aggcctgctt aagtcttctg tccgtgataa caacccagtt attatacttg
     5821 agtacaagtc agaatttaac caaaaagggg aagtgccagt tgatccagac tacacaattc
     5881 cacttggggt tggtgaaatc aaacgccaag gaacggatgt aacagttgtt acttatggaa
     5941 aaatgcttcg ccgtgtggtt caagctgctg aagaattagc agaagaagga atttcagttg
     6001 aaattgttga cccacgtacc cttgttccgc ttgataagga tatcatcata aactcagtga
     6061 agaagactgg taaggttgtt ctggtcaacg atgcccacaa aacaagtggc tatatcggtg
     6121 aaatttcagc tattatttca gaatcagaag catttgacta tctagatgca ccaatccgcc
     6181 gttgtgcagg agaagatgtg ccgatgcctt atgcacaaaa cctagaaaat gcaatgattc
     6241 caacagttga aagtatcaaa gatgcaatcc gaaaaactta taacaaagaa tagagttaca
     6301 taagataagt tatgtaaata aaataaagct gacgagaaac tttgtatctt ttaggtgcag
     6361 agctcttcgt cattaatatc ttatattcga gcacgggcta aaagctcgga aaaaagataa
     6421 atctccttgg cttcatcgaa gccatcgtcg atttcctatt tttcagtcgc ttttgacgcc
     6481 ctttgtatca taaatagaat tggagaggtc atggctgatg acaagctaag agcgactcct
     6541 gcagctagaa agttagcgga tgatttaggg ataaacctct acgacgtttc tggctcaggt
     6601 gcaaacggtc gtgtccacaa agaagacgtg gaaacttata aagacacaaa cgtggttcgc
     6661 atttcgccac ttgcaaaacg aattgccctc gaacataaca ttgcttggca ggaaatccaa
     6721 ggaaccggtc atcgtggtaa aatcatgaag aaggatgttt tggccctgct tcctgaaaat
     6781 atcgaaaacg atagcatcaa atctcctgct cagattgaaa aagtggaaga agtccctgat
     6841 aacgtaacac catatggtga aatcgagcgt attccaatga caccaatgcg taaggttatt
     6901 gcccaacgca tggttgaatc ttacttgact gcgccaacct tcaccctcaa ctatgaagtt
     6961 gatatgactg aaatgttggc tcttcgtaag aaggttcttg agccaatcat ggaagcaact
     7021 gggaagaaga ctactgtaac agaccttctt tcgcttgcag ttgttaagac tcttatgaaa
     7081 cacccataca tcaacgcttc attgacagaa gatggcaaga ctattatcac tcacaactat
     7141 gtcaatcttg ccatggcagt tgggatggat aatggattga tgacacctgt tgtttacaat
     7201 gctgagaaga tgagtctttc agaactggtg gtagccttta aggatgttat tggccgtacc
     7261 ttggatggta aattggctcc aagtgagtta caaaattcaa cattcacaat cagtaatttg
     7321 ggaatgtttg gtgttcagtc ctttggtccg attattaacc aacccaactc agctatcctt
     7381 ggtgtcagtt cgacaatcga gaagccagtt gtcgtcaatg gtgaaattgt gattcgccca
     7441 atcatgagtt taggattaac cattgaccac cgtgtcgtag atggtatggc tggtgctaag
     7501 tttatgaagg acttgaaaga gttgattgaa acgccaatct caatgttaat ttaagagcaa
     7561 gagtattcat ttttaagata tagataaagg aaggaagaca tggccttaga agtaattatg
     7621 ccaaaagccg gcgtggatat gacagaagga caaatcgtcc aatggaataa aaaagtcgga
     7681 gaatttgtaa aagaaggaga aatccttttg gaaatcatga ctgataaagt cagcatggaa
     7741 ttggaagccg aagaagatgg gtacttgatt gccattctca aaggagatgg tgaaactgtc
     7801 cctgtaacgg aagttatcgg ttaccttggt gaagaaaggg aaaacatccc aacagctgga
     7861 gcagcatcgc cagaagctag ccctgtacct gtagcaagta cctcaaacga tgatggtaag
     7921 agcgatgatg cttttgatat cgttgtgatt ggtggaggtc ctgctggtta tgttgcagcc
     7981 attaaagctg cccaacttgg cggtaaggtt gcccttgttg agaaatctga acttggtgga
     8041 acctgcttaa accgtggatg tattccaacc aagacctacc ttcataacgc tgaaattatt
     8101 gaaaatatcg gtcatgctgc aaatcgtggt atcgtgattg aaaatcctaa cttcactgtt
     8161 gatatggaaa aacttttaga aactaaatct aaagttgtta atactcttgt tggtggtgtt
     8221 gcaggacttc ttcgtagcta cggagttact gttcataaag gaattggtac aatcactaaa
     8281 gacaagaacg tcttggtaaa tggttctgaa ttgcttgaaa ccaagaaaat cattcttgct
     8341 ggtggttcaa aagtcagcaa gatcaacgtc tctggtatgg aatctccact tgtcatgact
     8401 agtgatgaca ttcttgaaat gaacgaagtg ccagaaagcc ttgttatcat cggtggtgga
     8461 gttgtcggta tcgaactcgg tcaggccttc atgacatttg gttcaaaagt gactgttatc
     8521 gaaatgatgg accgtatagt tccagctatg gatgcggaag tttctaagaa ccttcgcttg
     8581 atccttgagc gtaaaggaat gaccatcttg actggtacta aactgcaaga aatcattgag
     8641 gaaaatggtc aacttcgtat caaggttgaa ggaaaagacg atatcatcgc aagcaaagct
     8701 cttctttcaa ttggtcgtat gccagacctt gaaggtattg gagaggttga gtttgaattg
     8761 gatcgtggtt gtatcaaggt caacgaatac atggaaactt cagttccagg catttatgca
     8821 ccaggtgaca tcaacggtac taagatgttg gctcacgcag ctttccgcat gggtgaagtt
     8881 tccgctgaaa atgcccttaa aggaaatcat gcagttgcca aattgaattt gactcctgca
     8941 gccatctaca ctctccctga agtagcagca gtaggtttga cagaagaaca agcccgtgag
     9001 aaatacgatg ttgccatcgg taagtttaac tttgctgcta acggtcgtgc tattgcatct
     9061 gacgcagctc aaggtttcgt aaaagttatc gctgataaga aatacggaga aatccttggt
     9121 gtacacatta ttggtcctgc agccgcagaa ttaataaacg aagcatcaag catcatcgaa
     9181 atggaaatca ctgttgagga aatgctgaag accatccacg ggcacccaac ttactctgaa
     9241 gtgatgtacg aagcgtttgc agatgttcta ggaatggcca tccattcacc taagaaaaaa
     9301 taaaacgaag atagattaga ctggaaggtt atttccagtt tctatcatat aaagtaaagg
     9361 gatatcatga aatacattat caatcattca aatgacactg cttttaatat tgccttggaa
     9421 gaatacgcct ttaaacacct tttggatgag gatcaaatct tcctactttg gattaacaag
     9481 ccatctatca ttgttggtcg tcaccagaac actatcgaag aaatcaaccg tgattatgtt
     9541 cgcgaaaatg gtattgaggt agttcgccgt attagtggtg gtggagctgt ttaccacgat
     9601 ttaaataacc tcaactacac gatcatctcc aaagaagatg aaaataaggc atttgacttc
     9661 aagagcttct caactccggt tatcaatacc ctggctcaac ttggggttaa agctgagttc
     9721 acaggtcgta atgaccttga gattgatggt aagaagttct gtggcaatgc ccaagcctat
     9781 atcaacggcc gtatcatgca ccacggttgc ttgctctttg acgttgattt gtcagtcctc
     9841 gcaaatgccc ttaaggtttc aaaagataaa tttgaatcaa aaggtgtgaa atccgtccgt
     9901 gcccgtgtaa ccaatattat caatgaatta ccaaaaaaaa tcacagtcga aaaattccgt
     9961 gatttactct tggaatacat gaaaaaagag tacccagaga tgactgaata cgtcttttca
    10021 gaagaagaat tggccgaaat caatcgcatc aaggatacta agtttggaac ttgggactgg
    10081 aactatggta aatcacctga atttaacgtc cgtcgtggaa ccaaattcac tagtggtaag
    10141 gttgaagtct ttgctaacgt tactgaatca aaaatccaag acatcaagat ttatggtgac
    10201 ttctttggta ttgaagacgt tgctgcagta gaagatgtcc ttcgtggggt gaaatacgaa
    10261 cgcgaagatg ttcttaaggc gctaaaaacc attgatatca cacgctactt cgctggtatt
    10321 agccgtgaag aaatcgctga agcggtagtt ggataaatca aaaagaagtt ggccaactaa
    10381 ccaacttctt tttttgatat ttatttacat aatttaaaat acgtaaaatc ataaactatc
    10441 cagagcattc ttttgttcat cactaacaat atgggtatag aggtcagtga cttgtgtgct
    10501 agcatgtcct agttggtgac tgactaaaac ttgtgattta gtcgcatcat agagcctagt
    10561 tgctagtgta tggcgcagtt tatggggtgt tacacgcact ttaaaatcct ctgagtattt
    10621 agcaaccatt ttctcaacgc tagaagcatc gatacgatta ggaacacctc tgtagagagt
    10681 taaaaaaagg gctgtatctg ttttttccgt tttatagcgt tgattccgaa tggccagata
    10741 attctctaaa taaggtttag caaaagcagc gacattgact gagtcacgtt tgcaaccttt
    10801 tcgagtaaca tcaataacca tcatttttag attgagatct cttagatcta gattaacagc
    10861 ttcagataag cgaacaccag atgccaagag aagggcaata atggctaaat ctcgttcttt
    10921 atttttgttg aatgatgaga gagctcgatt tgaaagctgt tgtgggtgct cttgatcgat
    10981 ataagttaga aaaccttctg tttcatcacc tagaaagagt ttttgcttga tattttcagc
    11041 tctggcagca agggtttctt tctttttctt ggtggaaact tttttcatta cattacgata
    11101 gaaataaggt tccccctgat cgttttcaac ctcctcggtt agatacttgt aaagactaga
    11161 aagtgctgat aaggttcgat tgatagttgt ctgtgaaaca ccttgttttg ttgtattagc
    11221 attcagcaag ggacgttcac gtagataaag gataaaggat tccatgtctt tcttagacat
    11281 attttccaaa actgataaag gaatatcgga tattttatca gcgtttgaaa taccagactc
    11341 caaaacccag ctgaaaaatc ggtcatattc cttaaggtat tcgtacaggg ttgtaaaact
    11401 gtagggcaca gccagcttag attggtagta ttccagaaca taccagggca tgagttgttt
    11461 tagtttgtcg attcgttcca gtaaaatctc acgtttcatg tattttctcc ataagtaagt
    11521 at
//
LOCUS       DAVKZB010000058        10950 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKZB010000058 DAVKZB010000000
VERSION     DAVKZB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.058"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            225..410
                     /gene="briC"
                     /locus_tag="VXA80_001337"
     CDS             225..410
                     /gene="briC"
                     /locus_tag="VXA80_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV0353825.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            428..901
                     /locus_tag="VXA80_001338"
     CDS             428..901
                     /locus_tag="VXA80_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353826.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            914..1150
                     /locus_tag="VXA80_001339"
     CDS             914..1150
                     /locus_tag="VXA80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353827.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(1200..1622)
                     /gene="nusB"
                     /locus_tag="VXA80_001340"
     CDS             complement(1200..1622)
                     /gene="nusB"
                     /locus_tag="VXA80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV0353828.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(1615..2004)
                     /locus_tag="VXA80_001341"
     CDS             complement(1615..2004)
                     /locus_tag="VXA80_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0353829.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(2026..2586)
                     /gene="efp"
                     /locus_tag="VXA80_001342"
     CDS             complement(2026..2586)
                     /gene="efp"
                     /locus_tag="VXA80_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV0353830.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2715..4157)
                     /gene="gatB"
                     /locus_tag="VXA80_001343"
     CDS             complement(2715..4157)
                     /gene="gatB"
                     /locus_tag="VXA80_001343"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV0353831.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(4157..5623)
                     /gene="gatA"
                     /locus_tag="VXA80_001344"
     CDS             complement(4157..5623)
                     /gene="gatA"
                     /locus_tag="VXA80_001344"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV0353832.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAISSEDAKDSTSAPVRIADFTSKIGQGIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQRPVIFGGDN"
     gene            complement(5623..5925)
                     /gene="gatC"
                     /locus_tag="VXA80_001345"
     CDS             complement(5623..5925)
                     /gene="gatC"
                     /locus_tag="VXA80_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV0353833.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            6138..7682
                     /locus_tag="VXA80_001346"
     CDS             6138..7682
                     /locus_tag="VXA80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV0353834.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7951..8436
                     /locus_tag="VXA80_001347"
     CDS             7951..8436
                     /locus_tag="VXA80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV0353835.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            8552..8740
                     /locus_tag="VXA80_001348"
     CDS             8552..8740
                     /locus_tag="VXA80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV0353836.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            8897..9262
                     /locus_tag="VXA80_001349"
     CDS             8897..9262
                     /locus_tag="VXA80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0353837.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            9265..10932
                     /locus_tag="VXA80_001350"
     CDS             9265..10932
                     /locus_tag="VXA80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV0353838.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGTILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         3154 a         2067 c         2475 g         3254 t
ORIGIN      
        1 atccctattg acgacatcta taatttggat atttgtgtct ttgatatcga gtagttttgt
       61 gataaaatgt aattgttcca tatgaatctt tctaatgagt tgtttggtcg cttttcatta
      121 tagatcttat gggacttttt ttctacaata aaataggctc cataatatct atagtggatt
      181 tacccactac aaatattata gagcctttta agaaggtact tgcaatgaca ggtacagaaa
      241 catttacagt tatttcaact gaggacttgg agcaaacttc aggtggtctt gctgtttggg
      301 aagatggata tagtagatgg ttatattata gagaatttgc tccctatatg aggcaagggg
      361 cacttaattc ttatatagat gcttggaagt acggcttccg agcagggtaa tctttcaaaa
      421 aagtacgatg agaaacacaa aaaaactaag acaatttgga atatttttgc ttattatttt
      481 actatctacc tatttaccac aaacaattgg attgtatgtg acgatcattt tagggttagg
      541 agctgatgtc tattctctta ttttaacaat gggattagtg ggaagtttcc ttctattaat
      601 ttggaggtta aaaaagaaaa agatgctctt tatctttgag aaaaagagct ggaactggtc
      661 atttgtcttc tacttatttg caacttatgt ggtttatcag attctcggta atttttgggc
      721 acgctatgct catttgatta atcataggaa tattcatgat gagtatttta ccgtgctttt
      781 ttcaaatgga caaccaacct ttttatcgac tatcttatct tttgtcctgc ctgtaatcat
      841 cggtcctgtt tttgaggaga ctcttgatag agggtatttt atgaatacct tctttcccta
      901 gtccaagtac tatttggatg ttatcctatc tgctatcatc tttgggatta gccatttgat
      961 actatctcac agagatccaa ttagtttatt atattatagt ctgattggtt tcttttttgc
     1021 ccttgtttat cgttctaccg acaatctgag attaacgatt ctctgccata gtttttttaa
     1081 ctttctcaat catgcaaaac ctatctggat ttttgtttac aattatatct attatcattt
     1141 ttttagatag tgggaggcaa agaaaaaagt gtttgatatc tatcaaacac tttttctttt
     1201 tattgttctt ctgttacaaa ctggctgagc agtccattga taaaacgggc agatttttga
     1261 tcggagaagt cctttgcaag ctcgatagct tcattaacag caaccagctg aggagtgtca
     1321 aatgaagtga tttcaaagac tcccaagcga aggaggtttc tctccacgag cgttaagcgt
     1381 tcaatggtcc aacctgcttt taaatgctga gtgatttgct tatctagttc ttccttttta
     1441 gcttgaacac cagaaacgag gtctatcaaa aaggctggaa gttgtacatc cgtatcttca
     1501 cgatcatgag tataggcgaa acgacaagca gtttcgacat ccgtaccgaa ctcaaggctc
     1561 atgagagctt gaaaagcgca tttacggagt tggcgtctag attctaatag tggactagtc
     1621 attgaggaag tcctcgtcaa atagatcttt caattctggt tttggtgttt tatctgggac
     1681 gatacctgca acgtgaatat tgatagcagc gagttctaca tcagccatat tacggacggc
     1741 atctttgaca gctttctgga tagcaaccgc taccttagga acttttactc cgtactcaag
     1801 gtagagatag atatctgctg tgagttcttc gtccacgttt ttaagataaa tgccacggcc
     1861 gagtgaaagt tttgaaaggg tatcagacac tgatctgttt gaaaaagagt gaacaccctc
     1921 tacctttgca gtagcgatag caatgatttt ttcaagtaca cgtggggcga taacgatttc
     1981 gccaagttgt tcttcaattc ccatagaatg acctctttct agagattagg cacgagaaac
     2041 gtaagttcct tctgcagtgt tgataacgag tttttgtcct gcttcgatga agtctggaac
     2101 gtttacgaca agtccagttt ccatcgttgc tggtttacca gaacctgtaa cagtagcacc
     2161 tttgatagat ggttgagttt cagcaactgt caactcaaca gtagtaggaa cggtgacacc
     2221 gatcacttca gttccgtaga attggatttt cacatcagag ttttcaagga tgtaaagcaa
     2281 ttcgttttca acattgacta cagggatttc gtattggtcg taagtttctg tattcatgaa
     2341 gtatgctgtg tcatccattt tgtacaagta ttgagctggg acagtctcga taatagcttg
     2401 ttcaaatttt tcctctggac ggtagcttgt gtcaaatgta gaaccagtac ggacatcacg
     2461 caatttcata cgcatgatcg tgtttccttt acctggtttg tggtgactag cttccaaaac
     2521 gcgaatcaat ttgccgtcag ctgtttcaaa ggtcatacca gcttttaatt tacttgcttc
     2581 aatcatgttt ttacctcttt aataaatatt attactcttc attttaccat aaaatactct
     2641 ggaaataaag ccaaaatagt tattgggagt tggtagagaa gaaaaaacca accttagggc
     2701 tggttttgtt ctgtctagtt ttctttcaac ttcgccaatt cctgtgcaag tagtttaagg
     2761 gcaacttgtg ggttggcttg gcctttggtt gccttcataa ggaatcctgt aaaggccttg
     2821 tcggcgttac gtttgcctga cttgaagtcg gcaacagcag cttcgttatc ggcaaagact
     2881 tggtggatga ttgggatcaa gatagctgga tctgaaattt gaaccatacc tgctttttcc
     2941 acgtattcac gcgcgccacc gccattttta gctagatgga caaagacttt cttggcaatc
     3001 ttagatgaaa tagtaccgtc ttcgatgatg gcaatcattt caaccaagtt ttctggtgtc
     3061 aattcgattt gttccagtgt tttaccttca gcattcaaga actgagcgac ttccccttgg
     3121 agccagttag agacttgttt ggcatcacca tctagagcaa cagctttttc aaagaaatca
     3181 gaagtgactt tattagcagt caactgacta gcatcgtagt ctgataaacc aaggtcagat
     3241 acataacgcg cacgacgttc ttttggaaac tctggcaact cagtccgcat ttcctcaatc
     3301 cactcgtcag aaatttcaaa gaggggtagg tctggttctg ggaagtagcg gtagtctgca
     3361 gccccttcct tgacacgcat gagaatggtt gctttattcg cttcatcgta acggcgtgtt
     3421 tcttggcgga tttgaccacc tgagcgaaga atttcagcct ggcgttggac ttcgtattca
     3481 agacctttac gaacgtttga gaaggagttg aggttcttca attcagtctt ggtaccgaat
     3541 ttttcttgac cataagggcg aagggagatg ttggcatcca cacgcataga accttcttcc
     3601 atcttaacgt cagaaatgcc agcgtactgg ataacttcct tgagggctgt cagataagca
     3661 taggcctcct ctggcgaacg catatctgcc tcagatacaa tctcaatcaa gggaacccct
     3721 tggcggttga ggtcaacata agagtagcca tctgtaccat gggtgttttt accagcgtct
     3781 tcctctaggt gggcacgttc gataccgatt ttcttggtcg taccgtcttc tagtttgact
     3841 tcaatccagc cattatatcc gattggttca tcaaattgag aaatttggta ggctttgggg
     3901 ttatcaggat agaagtagtt cttgcggtca aagtgcatct ttttgtggat gtccatgttg
     3961 agggcaagag cagccttgat accggcatca acaacccctt tattgagaac tggtagaact
     4021 cctgggaaag accagtcaat cacgttagtg ttggcatttt ggtcatttcc gaagtgggca
     4081 gaagtaggtg agaagatttt tgaattggtg ttgagctcta cgtggacttc aagtccgatg
     4141 actgtttcaa agttcattag ttgtcacctc caaaaatcac gggtcgttgt ttgtggtagt
     4201 ctgttgttgc ttcaaaagca gcagcagctt ggtaaatggt ttcctcagag tacttgggac
     4261 caatcaattg gagtccgaca ggtagacctt gagagaatcc agcaggaatc gaaattccag
     4321 gcagtcctgc caagtttaca ggtatggtca ataggtcggc taagtacatg gcaactgggt
     4381 catggttgag agaatccaag tcataggcaa cacttggagc agttggaccc aaaatcaaat
     4441 cgtaatccgc gaagactttt tcgaaatctt gaatgataag tgtacggacc tgtccagcct
     4501 tcttatagta ggcatcgtag taacctgatg aaagactgaa agtacccagc atgatacggc
     4561 gcttcacttc ttcaccaaaa ccttggcttc ggctgtttac atagatttca tcaaggttgg
     4621 ttgcatcttc tgcgcgatag ccgtaacgga taccgtcaaa gcgttgcaag tttgatgaag
     4681 cttctgatga agcgatgatg tagtatacgg caactccgta tttagagtga ggaaggctga
     4741 cttcttcgac aatagcaccc aatttttcaa agtgtttagc ggcatttaga atggtttcct
     4801 taacctctgg gttaattcct tcaccgaggt attccttagg caaagcgatt ttcataccct
     4861 tgatgccttg gccgattttt gaagtaaagt cggcgatgcg gacaggagca gaagtagagt
     4921 ctttagcatc ttcgctggaa atagcgttga gcaagagggc attttcctta acagtaggag
     4981 caaaaggtcc aatctggtct aatgagctac caaaggcaat gagaccgaaa cgtgaaactg
     5041 ttccgtaggt tggtttgaga ccaacgattc cgttgaaggc agcaggttgg cggatggaac
     5101 caccagtatc agaaccaagt gacaagcgaa cttgtcctga ggctacagct gcggcagaac
     5161 cacttgatga cccaccagga accttgctgt ggtcccaagc gtttttagtt gctccgtagt
     5221 gtgaagtttc acctgaacca cccatagcaa attcgtccat gttggtcttt ccaacgacaa
     5281 tcatgccctt ggtttttgca ttggcaacag ctgtcgcatc aaagattggc tcatagttgt
     5341 agagcatttt tgaggcagca gttgtaagaa taccgtctgt agagatgtta tccttaacag
     5401 caagtggaat tcctgaaagg acattgtcag catcaattcc agcttcatca atggctttag
     5461 cttgaacaag agcttgctcc tcagcgatgg tgacaaatga attgagggct tcctcacgag
     5521 acttgatatt ttcaagtgtt gcttgggtca attctgttgc agaaatttcc ttagagacaa
     5581 ggagattgtg caactcttca atagttttat tgttaaaagt cattaggcat ctcctccatc
     5641 gtctaggata gctggcacct tgatatagta gttgtctttt tcaggtacgt ttttaaacaa
     5701 gcgatcacgg tctgttcctt cttcggccac atcagggcgg agtacagtct tgcggtcagc
     5761 catagtcgta gtaggtgcga caccagttgt gtcaacttcg cccagcaatt caaccatgtc
     5821 aacaatctta gacaaggtgg tcgcaaaggc agcagtttct ttttcagaga atcttaattt
     5881 tgaaagattg gcaacgtgtg ttacctcttc ttgcgtaatt ttcatcttgc atcctttcgt
     5941 gaaatgatga tttttagtct gttctatttt accatatttt cctataaata agggaggggg
     6001 aagtgaaaag aaatagaaat tgaaaaaaat gctaaaatcc gatataatgg agtgttgaaa
     6061 ggaatggtaa aatccaatgt aaaaatcatt ctcagctatt gaaacaagaa aaatagagaa
     6121 tcaaagaaag agaacttatg aatattcaag aagaaattaa gaaacgtcgt acctttgcca
     6181 ttatctccca cccggacgcg gggaaaacaa ccatcactga gcagttactc tactttgggg
     6241 gtgaaattcg tgaggctggt acggtaaaag ggaagaaaac agggactttt gctaaatctg
     6301 attggatgga tatcgagaag caacgtggga tttctgttac ttcatctgtt atgcaatttg
     6361 actacgacgg caagcgcgtg aatatcttag acacgccagg gcacgaggac ttctcagaag
     6421 atacctatcg taccttgatg gcggtggatg ctgcggtcat ggtcgtggac tctgccaagg
     6481 ggatcgaggc tcaaaccaag aaattgtttg aggttgtgaa acatcgtggc attccagtct
     6541 ttacctttat gaacaagctg gaccgtgacg gtcgtgagcc tttggatctc ttacaagaat
     6601 tggaagaaat cttgggcatt gctagctacc ctatgaactg gcctatcggg atggggaaag
     6661 cctttgaggg cttgtatgac ctctataacc aacgtttaga gctttataaa ggggatgagc
     6721 gttttgctag tctagaagat ggggacaaac tttttggtag caatcctttc tacgagcaag
     6781 tcaaggatga tattgagctt ttaaatgaag ccggaaatga gttttcagag gaagctattc
     6841 tggctggaga attgacgcct gtctttttcg gttcagccct gacaaacttt ggtgtgcaga
     6901 ccttccttga aaccttcctc aagtttgctc cagaaccaca tggtcacaag aaaacagacg
     6961 gtgaaattgt ggatccttat gacaaggatt tctcaggctt tgtctttaaa atccaagcca
     7021 acatggatcc tcgtcaccgt gaccgtattg cctttgttcg tatcgtatca ggcgaatttg
     7081 agcgtggcat gagtgtcaat cttcctcgta ctggtaaggg ggccaaacta tctaatgtta
     7141 cccagtttat ggcggagagt cgtgagaatg tgaccaatgc cgtggcaggt gatattatcg
     7201 gggtttacga taccggtact tatcaggttg gggatacctt gacggttgga aaaaacaagt
     7261 ttgaatttga acccctgcca acctttactc ctgaaatttt catgaaagtt tctgctaaga
     7321 atgttatgaa gcaaaaatcc ttccacaagg ggattgagca attggtgcaa gaaggagccg
     7381 ttcagcttta taagaattac caaacaggtg agtacatgct aggcgctgta ggtcaactcc
     7441 agtttgaagt ctttaaacac cgcatggaag gcgagtacaa tgcggaagta gttatgaccc
     7501 caatgggtaa aaagaccgtt cgttggatta agcctgagga cttggatgaa cggatgtcgt
     7561 caagtcgcaa tatcttagcc aaagaccgtt ttgaccaacc agtctttctc tttgaaaatg
     7621 actttgccct ccgctggttt gcggacaagt atccagacgt agagttagag gagaagatgt
     7681 gactcagtac caacaattgg aactaaagtt ccaaattgtt ggacgctagt cgctatttgg
     7741 cgaactagct aactgcctca ctaactacgt ttggcaaatg aaatcgattt gtaaaactcc
     7801 gtgtcataat gtaaaaattg taaaccgtaa tgggacttca tctaaaatct ccactggtat
     7861 ggttttacga acgaataggc gaaaataatg gttacgtaga aacatatgat gggaataata
     7921 tgatgggaat aaaaagaaat aagataaaga gtgaaagaag agaaaaaagc gattgtctta
     7981 ggtgcagata atgcttatat ggataaagtt gaaacaacaa taaaatctct ttgtgttcat
     8041 cattataatc ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg
     8101 atggaaaaaa gactagagac tttgaatagt gagattgtga atgttcagat tgacagtagt
     8161 attctgaaag gttatcgtct cccctttgag ggactttcat atgcggcttt ttttaggtat
     8221 tttattccaa agtatgtaag cgagtctcgt gtactgtatt tggattcaga tattgttgta
     8281 agaaaatcga tagatgagct gtgggatttg gacttgacgg ctataccctt agctgcagtt
     8341 agagatgatt tttatacaca taattttaat tctggtgttc tgttaatcaa caatggcatg
     8401 tggagagcag aaaatgtcac gcaagacctg atttaagaca caaataaaaa atcttccaat
     8461 tcccttgcaa aaaacaagaa agtgtgataa catagtacgg tatgtggtgc tagcacatcc
     8521 gctatattag atctaatagg aggaaaacac aatggctaaa gtatgttact ttacaggtcg
     8581 taagactgta tcaggaaaca accgttcaca cgcgatgaac caaacaaaac gtgccgtaaa
     8641 accaaacctt caaaaagtta ctgttcttat cgatggtaaa cctaaaaaag tttgggcttc
     8701 agctcgtgct ttgaaatcag gtaaagttga acgcgtttaa taataaaagc aaaggagacc
     8761 ttagggtctt ttttctttta ttgtgggtat aacatcgttt gaaaaataga gtaaatatcc
     8821 gctgatacat cattctgctt ttacacttgg gctgaaatat gataaaatag agtatcaact
     8881 agttgaggta aaaaggatga ctgtaaaaat taatacaaaa gatggtcaaa tcgaactaac
     8941 agatgaagtg attgcaaccg tcgtaggtgg agcagcaact gagatttttg gtgtggtcgg
     9001 tatggctagt aaaaatgccc tcaaagataa tttccaagcc ctgctaggta aggaaaatta
     9061 ttctaaaggt gtcgtcgtaa aggcagccga agatggcagt attgcagttg atgtatatac
     9121 cgtgttaagc tacggaacaa agattagtga agtgtcaaaa aacattcaag agcgtgttgc
     9181 ctttagcttg gaaaaccagc ttggaattac tgctcagact gtaaatgtct acattcaaaa
     9241 tatcaaagtt gtaggagaat aaccgtgtca aaaattacta ctagcttatt tcaagaaatg
     9301 gtgcaggctg catcaactcg cttgaataag caagctgaat atgtcaattc attaaacgtc
     9361 tttccagttc cagatggaga tactgggaca aatatgggaa tgaccattga aaatggtgct
     9421 aaagaagttg cagacaagcc agcttctaca gttggagaag tagcgagcat tcttgccaaa
     9481 gggcttttga tgggtgcgcg tgggaactca ggagtgatta catctcagct tttccgtgga
     9541 ttttcccaag ctatcaagga taaagacgag ttaacaggtc aagacttggc tctggccttc
     9601 caatcaggtg tggaagttgc ctataaggca gtgatgaaac ctgttgaagg aacgattttg
     9661 acagtttctc gtggagctgc tatcggtgct aagaaaaaag ctgagcaaac agatgatact
     9721 gttgaagtca tgcgcgcagc cttggaaggt gctaaaacag ctctagctaa aacaccagac
     9781 atgcttccag tattgaaaga agttggcgtt gtggactcag gtggtcaagg attggtcttc
     9841 atctacgaag gtttcctttc agcccttact ggcgaatata ttgcatctga ggactttgta
     9901 gcgactcctg ccaacatgag tgagatgatc aatgtagagc atcataagtc tgtagctggt
     9961 cacgtagcga ctgaggacat cacgtttggt tactgtactg aaatcatggt agctcttaag
    10021 caaggtccaa cctatgctaa agattttgac tacgatgaat tccgtaacta cttggatgag
    10081 cttggggatt ctctcctcgt tgttaacgat gatgaaattg tcaaagtcca tgttcataca
    10141 gaagatccag gacttgttat gcaagaaggt ctcaaatatg gtagcttggt caaggtaaaa
    10201 gttgacaata tgcgtaacca acacgaagca caggttgaga aagaagctac tcaagttatc
    10261 aagtcggctg aagaaaaaga gtatgctttg attgctgtgg tggctggtaa aggtctagca
    10321 gatatcttct gttctcaagg cgtggattat gttatcgaag gcggtcaaac catgaaccct
    10381 tcaacagaag actttatcaa ggctgttgaa caggtcaatg cccgtaacat catcttcttg
    10441 ccaaacaaca agaacatctt catggcagct caatctgcgg cagaagtttt ggagcaacca
    10501 gcagtagtgg tagaggctcg cactcttcct caaggtatga caagtcttct tgcctttgat
    10561 ccaagcaagt ccattgaaga aaatcaagag cgtatgacag ctgctcttag agatgtcgtt
    10621 agcggaagcg tcacaacagc cgtgcgtgat acaacgatcg atggcttaga aatccatgaa
    10681 aacgataatc taggtatggt ggatggaaca attcttgtgt caaaccctga tatgcaccaa
    10741 acattgactg aaaccttgaa acatatgttg gatgaagaca gtgaaatcgt aaccttctat
    10801 gtcggtgaag acggaagcga agaacttgcc aatgaaatcg ctcaagaaat cgtagaagaa
    10861 ttcgaagacg ttgaagtcga gattcaccaa ggtcaacaac ctgtttaccc atacctattt
    10921 agtgtggaat aagattaatg aaggactgag
//
LOCUS       DAVKZB010000059        10602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKZB010000059 DAVKZB010000000
VERSION     DAVKZB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.059"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(50..1372)
                     /locus_tag="VXA80_001351"
     CDS             complement(50..1372)
                     /locus_tag="VXA80_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV0353839.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYIFLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1463..1551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1715..4051)
                     /locus_tag="VXA80_001352"
     CDS             complement(1715..4051)
                     /locus_tag="VXA80_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV0353840.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLTSREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4155..4703)
                     /locus_tag="VXA80_001353"
     CDS             complement(4155..4703)
                     /locus_tag="VXA80_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV0353841.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIAYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4700..5002)
                     /gene="zapA"
                     /locus_tag="VXA80_001354"
     CDS             complement(4700..5002)
                     /gene="zapA"
                     /locus_tag="VXA80_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV0353842.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5089..5970
                     /locus_tag="VXA80_001355"
     CDS             5089..5970
                     /locus_tag="VXA80_001355"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV0353843.1"
                     /translation="MASITLTPSEKDIQAFLEHYQSSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSTKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSIIARNLFLENLENLGRELGYQLPSGAGTTSDKVASQILQAYSMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            5974..6588
                     /gene="lepB"
                     /locus_tag="VXA80_001356"
     CDS             5974..6588
                     /gene="lepB"
                     /locus_tag="VXA80_001356"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV0353844.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            6716..9082
                     /locus_tag="VXA80_001357"
     CDS             6716..9082
                     /locus_tag="VXA80_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV0353845.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTIADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9130..10413)
                     /locus_tag="VXA80_001358"
     CDS             complement(9130..10413)
                     /locus_tag="VXA80_001358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV0353846.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3029 a         2426 c         2147 g         3000 t
ORIGIN      
        1 gataaaaatt atttataggc aaaaaaatca cacgagctgt gtgattccat tatttgtcaa
       61 aatacttttt agtttcagca ataacgactg gcgacaagac caagagggca atcaagtttg
      121 gcagagccat caaggcgtta acgatatctg cgataatcca gaccatatcc aactcgataa
      181 atcctcctaa caagaccatg agcacaaaaa ccacacggta gagccagata aagcgagccc
      241 caaagaggaa ctcaaaacag cgttctccgt aatagttcca acctagaatc gttgtaaagg
      301 caaaaagtac aaggaagatg gtcaagaggg caggcccaaa gtgtgaaaag actgttgaga
      361 aagctgactg agtcaaggca accccattca agtcaccact ccaaactcca gttaccaaga
      421 tggtcaaacc agttagagta caaatgatga gggtatcaat aaaggttcct gtcatggaaa
      481 tcaaaccttg ctctactggt tcatttgtct tggcagctgc agctgcaata ggagcagaac
      541 ccagaccaga ttcgtttgag aacacaccac gcgccacacc attttgaata gccatccgaa
      601 cgctagcacc agcaaatcca cctaccgcag caaggggact aaaagctgag gtaaagacta
      661 aagcgattgt gccagggatt tttccgatat taaagaaaat aactgtaaga gttcctaaga
      721 tataaatgag ggccataaaa ggaacaacag tagttgaaac cttagaaata gacttgagtc
      781 caccaaagac tgcaatcgct acaaagacag acaagacgag agctgtgatg gctggcgaaa
      841 tcgtcgttgt attttggata gattctgtaa tcgagttgac ttgggtgaag gttccgattc
      901 ccaagagagc aaccaatact cctgctactg caaacaaaac agcaagtggt cgccactttt
      961 ctcccatccc tagaaagata tagtgcatgg gacctcccgc tactgcacca tggtcgtcct
     1021 tggtgcggta tttgatggcc aagagtcctt ccgcatactt ggtagccatt ccaaagaaag
     1081 ccgccatcca catccaaaat agagctcctg gtccaccaac cttgatagcc gtcgcaactc
     1141 ctatgatatt tcctgttcca acagttgatg ccaaggctgt acacagagct gcaaaactgg
     1201 atacatcacc atgtccctta tcctggataa aaataagctg aaaggccttg ggcagacgca
     1261 aaacctgcaa gagtcctagc cgcatggtta ggtaaatccc tgttccgacc aataaaatca
     1321 agaggggcgg tccccaagca aaagcatcga ttgatttaag caattctaac atttccttct
     1381 cctatcgttt caaccccaaa agaaagagca catgcaagat acatgtactc tggaatgctt
     1441 agataaatgc taaaaagcgg tctatcctag ctctgtcctt ttacctgaga gtttgagcag
     1501 ttgcctgcct tgccccttcg gtgcctttac ggtctctcca gagttccgtc catttacagt
     1561 catggaaaat caaacgattc cccacttcta ttaaacttca ttcggtgttg gtatttaatt
     1621 gattctaatt ttacaaaaaa tgttggcttt tgtcaatgtg tttattagta aaaattagtt
     1681 caacagtttt tactttataa agtccagaat actgctatcc tttaaaagtg acaatagtcg
     1741 caccactgcc tccagcattt tgtggggcat agccgaaact cttgacatgt ttgtttcttt
     1801 gcaagtattt ggtaactcct tcacggatga ctcctgttcc gataccatgg atgatatcaa
     1861 cttgagccat attgttaagc aaggcttggt cgatgaaggt atctagctca ttcatggctt
     1921 cttcatagcg cttgcctcga agatccagtc tagcttgagg tcctcgccca gaagttcgtt
     1981 tcacaacatt gacctgtttc ttcttgactg gtttttcttg ctgggcttga acaagatcaa
     2041 actctttctc ttccaaggtc atcttaatca agccaacttg ggcttcccag cgaccgtcct
     2101 tgagttgact ggtcaaggta ccacgctgac cataactgag aaccacaata tcatctccca
     2161 cctttggagc tcgttttttc ttggcctttt gaaggacctt atttttagac aagtccactt
     2221 tttcaggagc caattttttc aacttggcct tggcttcaat gatttcgtgg ggcttgagtt
     2281 gggatttact gtggagattt ttgagaatct ggtcactttc acttagggcc atatccacaa
     2341 tctcagcagc ctgttcacgc gccttgttaa gctcggtttc cttttcacga ttaagctcgt
     2401 tgtagagttt ttttagcgca cggttcatct tgagattttc ttgctccacc tcacggatat
     2461 tgtccaaacg tttgcggctt tccagcgtct gctcttctaa ttgctcaatg atacgattga
     2521 cgtcattgtc ctgatcgatc tgctgactgg catctcctac gataacttca gataggccta
     2581 gacgtttggc aatttcaaag gcattacttc ggccaggaac accctgcata aagcgatagg
     2641 tcgggcgaag agttgcagta tcaaactcca tactggcatt ttgcacaaag gctgtctcaa
     2701 taccgtaggc cttgagttct ggatagtggg tcgtcgccat ggtcttgatt tgacgcaggc
     2761 gaaggtcctc cagaatagcc atggcaaggg ctgctccctc ttggggatca gtaccagccc
     2821 ccaactcatc caaaagtaag agtgaatgtt ggttgacctt gccaagaata tccacgatat
     2881 tggtcatatg gctagagaag gtagacaagc tctgctcaat agactgctca tctccaatat
     2941 cagcaaagat ttcttcaaaa ataccaacac gacttccctt gtctgctaaa atcggcaatc
     3001 ctgactgggc catgacctgt gtcaagccca gagttttgag catgatggtc ttcccacctg
     3061 tattgggacc tgtaatgaca atagctgtta aatcttgacc aaaatagaca tcatttgcga
     3121 cggcattttt gaccaaagga tggcagacat ggagcagttg aatctcttga ttttctgaca
     3181 gctgaggcac gactgcttgt ctttcttgga taaatcgaac cttggcacga atcaagtcca
     3241 gatgaccgat aatccaagcg tcattagcaa tctcagccgc atgagggcgg acacgctcag
     3301 aaatttcttg gagaatgcga agcatttcat agcgctcatc tgctcgcaga ctagcaattt
     3361 cttcgctcag tttgactacc tcacgtggtt cgatatagac ggtgtttcca ctagcagaaa
     3421 tatcatgaac gacacctgca atcttattgc ggtaggtgtt tttgactggt aaaacctgac
     3481 ggccatttct gctagcaaca attccttccg tcaacatctg cgctttttgc ttgagcaagt
     3541 cttgtaaaac atcgcgtacc tgactctcgc tatcatgtat ttttcgacgg attcgcgcca
     3601 attcttcact ggcaaaattt tcaatgaaac ccgcatcatt aaaggcctga agatttcctt
     3661 gtaattgcgg aaaatcatgt aatttctcaa accaaagggc taattcttcc aagctgacat
     3721 tttccagatt ggcgtaaaaa ctttgaagtt ctcggctggt aagaagcacg cgtttcaaga
     3781 gtaggaactc ctcgatattg agatccgctc ccatctccaa cctcttgcag actcctgcaa
     3841 tttccttagt tgagagaata gtaaaatgcg gttgctcgac gaaaagagcc tgcatttctt
     3901 tcatctcagc aaaagcctgt ttgattttat ctgctttggc agtcggagcc agctgtctca
     3961 attgctccaa gccctgctcg gtcaacaaat gaggctcaaa caaggccttg accttatcga
     4021 actctaatgt ttctaatatt ttcttattca tctttatttc cttgtctttg aatgtctagg
     4081 tgtggtagct ttttacattc tgatagattc gagtcaaatc tgtaaacaaa gggctaggaa
     4141 aactcctgcc ctttttatcc gattaaattt gtcacccaga tttgtttgag ccaactggtt
     4201 gttatcggta tgctctggat gatgtgtttt gcgacgatac tcttttcaag aggattttgt
     4261 ataactgcca tggggatggt cgccaagatt gtcaaggcca tttgcaagac aaataaggtc
     4321 accaacatgg acaagatacc tgctgaaact tggaacaact taccacccag ttttttgcta
     4381 ggaatcaagt gtaagagaag accaagcaaa cgaccaatgc tatagacaat cccaaataca
     4441 agcaagtagg cgatacctgc ataaaagacc ttatccagct gaaagagttg atccgatggg
     4501 aaaaagaaag tcccctgacc ttcctgcgaa tttgcataag ggagcaataa atggaattgc
     4561 tctccaagcc ccttataaaa ctggccagcc ataaaagccg atgccatggc tgaaatcagg
     4621 taataaacct gtaagagcag gcctctccga tagccgatat aaaatcccca aaccaagacc
     4681 aatagaagaa ggaatgaaat cataaggaat cctcaatctt gctctgttct tgcttacaag
     4741 tcacaagctt gtgacggagt tcttctagct cttgctcctt atcgtcaaat tcaatctcac
     4801 ggctgagctg agttgataaa cagttgactg ccaacaaaag agcgattgtt tcatcatctg
     4861 cgctaggcat ttgttcttta attgcttggt atttttctgt cgcaacctta gcgatttcct
     4921 ccataaaaag gttatcatgt tcgcttgtca aggttaacga ttttttcccg aatgtaaatt
     4981 tgaatcgatt tagatttgcc ataaaattca cctcacgata ttataccaaa attcgctaat
     5041 tttgtcagtt tttacaaatt ttactgcttt tgtggtacaa tagaaactat ggcaagtatc
     5101 acactcacac caagcgaaaa ggatattcag gcttttcttg aacactatca aagcagtctg
     5161 gctcctagca agaatcccta tatccgctac tttttgaaac tacctcaagc aacggtttct
     5221 atctatactt ctggaaaaat cttgcttcag ggtgaagggg ctgaaaaata cgccagtttc
     5281 tttggctatc aagctgtaga gcaaaccagc ggacaaaatc ttcctttaat tgggacagat
     5341 gaggtgggaa atggttccta ctttggtgga cttgcagttg tggctgcctt tgtcacacct
     5401 gaccagcatg ccttcttacg aaaactcggt gtgggggatt ctaagactct gaccgaccaa
     5461 aagatccgtc agattgcccc tatcctcaaa gaaaaaatcc agcaccaggc actgctgctc
     5521 tcaccaagca agtacaacga ggtcatcgga gaccgctaca acgctgtttc ggttaaggtt
     5581 gccctccata atcaggctat ctatctcctc cttcaaaaag gtgttcagcc tgagaaaatt
     5641 gtgattgatg cctttaccag tactaaaaat tatgacaagt acttggctca agaggccaat
     5701 cgtttcagca atcctatcag cttagaagaa aaggctgagg gcaaatactt ggctgttgca
     5761 gtttcttcca tcattgcgcg taatctcttt ctggaaaatc ttgaaaatct gggacgagaa
     5821 ctgggctatc aacttccaag tggagctgga acgacttccg ataaggtggc tagccagatt
     5881 ttgcaagcct atagtatgca gggactcaac ttctgcgcca aattgcactt taaaaacact
     5941 gaaaaagcga aaaaacgctt agaaaggtaa gttatgaatt catttaaaaa tttcttaaaa
     6001 gagtggggat tgttcctcct aattctgtca ttactagctt taagtcgtat ctttttttgg
     6061 agcaatgttc gcgtagaagg acattccatg gatccgaccc tagcggatgg cgaaattctc
     6121 ttcgttgtaa aacaccttcc tattgaccgt tttgatatcg tggtggccca tgaggaagat
     6181 gggaataagg acatcgtcaa gcgcgtgatt ggaatgcctg gcgacaccat tcgttacgaa
     6241 aatgataaac tctacatcaa tgacaaagaa acggacgagc cttatctagc agactatatc
     6301 aaacgcttca aggatgacaa actccaaagc acttactcag gcaagggctt tgaaggaaat
     6361 aaaggaactt tctttagaag tatcgctcaa aaagctcaag ccttcacagt tgatgtcaac
     6421 tacaacacca actttagctt tactgttcca gaaggagaat accttctcct cggagatgac
     6481 cgcttggttt cgagcgacag ccgccacgta ggtaccttca aagcaaaaga tatcacaggg
     6541 gaagctaaat tccgcttatg gccaatcacc cgtatcggaa cattttaaga aacctaagag
     6601 gccgagaatc accaatctca gcctcttctt ctatcgtgag aaaatgattg gtaactatct
     6661 aaacttacca gaacagaaac acctcaactc tcacctattc atgcaaagga attttatgga
     6721 agtttatttt tcaggaacca ttgaacggat tatttttgaa aatcccagca atttttatcg
     6781 catcctcctc ctagaaatcg acgatacgga cgcagaggat tttgatgatt ttgaaatcat
     6841 tgtcacagga accatagctg atgtaattga gggcgaagac tatacttttt gggggcaaat
     6901 tgtccagcac tccaagtatg gagaacaact gcaaatcagt cgttatgatc gcgcaaaacc
     6961 aactagtaag ggcttggtca agtacttttc aagtagccat ttcaagggga ttggtctcaa
     7021 gacagctcag aaaatcgtgg atacctatgg cgaaaatacc attgacgaaa ttttgcaaca
     7081 cccagaaaag ttagaaggca tcgcaggact ctctgccaaa aatcgcgagg ctttcgtctc
     7141 cactctccgt ctcaactacg gtacggagat ggttttggcc aaactagcca actacggcat
     7201 tcccaacaaa ctagcctttc agattcaaga cttttacaag gaagaaaccc ttgatgtggt
     7261 tgaaaattat ccctaccaac tggttgagga tatcaagggt ttgggcttta ccattgctga
     7321 ccaactagct gaggaactag gcatcgaaag tcaggctcct gaacgcttcc gcgccggtct
     7381 agttcacagt ctttttcagg cctgtatgga aacaggagac acctatgttg aagcacggga
     7441 tttgctggaa caaaccctta ctctccttga gtcttcccgt cccgtggaac tagaccccag
     7501 ccaagttgcc caagagctct cctacctgat cgaagaagac aaggttcagc agattgatac
     7561 caagatcttt gacaacagcc tctttttcgc tgaggaaggc atccgcagtc acttggttcg
     7621 tatccttgaa aaaggaaaac agaagagtca tgatttagaa accattcaaa aacatatcac
     7681 tactatcgag gaagagctgg gaattgagta tgataacatt caaaaacagg ctatctgtga
     7741 tgctatccag aataaggtct ttatcctgac aggtggacct ggtactggta agacaactgt
     7801 tatcaatggg atcatcgctg tttacgcctt gttagaggga cttgacctca ggaagaaaag
     7861 caacctgccc attcttcttg ctgctccaac tggacgagcc gctcgccgca tgaatgaatt
     7921 gacaggtttg cctagcgcga ccatacaccg ccacttggga atgacaggtg acgatgatac
     7981 cagtcatctg gaagattacc tagatgctga ctttatcatc gtggatgaat tctctatggt
     8041 ggatacttgg ctggccaatc aactcttctc caatatctct tctaacagta agatcctcat
     8101 cgtaggtgac agtgatcagc taccttctgt cagtcctgga caagttctag cggatctgct
     8161 tcatattcct ttgattcctc agactcgctt ggaaaaaatt tatcgacaaa gcgaagaatc
     8221 aaccatcgtc accctagcta gtcagattcg acagggcatc ttgccagctg atttcaccca
     8281 aaaaaaagct gaccgttcct actttgaaat tgctagtggc catattcctg ccactattga
     8341 aaaaatctta ggtgccgccc tcagaaatag tattcctgcc cgtgatatcc aagttctggc
     8401 tcctatgtac cgagggacgg cagggattga tgctatcaat cagctcatgc aagacctgct
     8461 caatccccca caaaaagatc aactcagttt tgaagctcct cagtgccact atcgtaaggg
     8521 agacaaggtc attcatttgg ttaacgatgc tgaaatcaat gtctttaatg gggatttagg
     8581 agctatcaca gacctgattc ctggtaaata caccgaatcg aaacaagacg agattgtcat
     8641 tgattttgat ggtaatgaag tctcttaccc ccgtaacgaa tggtacaaga ttcgcttggc
     8701 ctatgccatg agtattcata agtctcaggg aagtgagttc ccagttgtca tcctaccgat
     8761 tactagtgct agccgacgta tgctggagcg aaatctcatc tacacagcca ttacacgtgc
     8821 caaaagcaaa ctcatcttac taggcgaatt acaagccttc gactatgcta cccaacacat
     8881 cggaactgcc cgaaaaacct atctgattga acgcttcagt gatctactgg agaatgttga
     8941 agaaaagcaa caagctgtct ctgaaacagt cacatcaagt gcctctgaac aatcctacat
     9001 cctaaccgaa gaaaactggg acagcattcc agccatgatt gggattacag acacagacct
     9061 caaagagatt tttggaaaat agtacatcag aaaacccacc aattcaggtg ggttttctgt
     9121 ttattaagat tattttactg ttgctgtgct tgtgatcaat tcaacagctt ttttgatagt
     9181 gatatcatgt ttcaacatgt cagctgaaag caagttttga acttgtgcaa cttccatgtt
     9241 gtagtctgct gccaattgct caacttcttt ttggatttct tcttctgaag catcaaatcc
     9301 ttcagctttg gcaactgctt cgataacaag gttagtctta gtacgtgact cagcttctgc
     9361 ttggtattgg ttatgaaggt cttcttgagt agttccagtg atttggaagt acatgtcagg
     9421 gttgatccct tgacgttgca agttcccaag gaattcattt actgaacggt gaacttcttc
     9481 gtggatcatt tcttctggaa gttctacgat ttcagcgttt tctacagctg tatcaattgc
     9541 tgcaccttca actgcatctt tgtaagcttc ttctttagca gcagccaatt ctttgcggta
     9601 tttttctttc aagtcagcaa gtgtttcaac ttcttcatca atgtcttttg caagttcatc
     9661 gtcaagagct ggaacttctt tagcttttac ttcgtggata gttgtcacga atttagcttc
     9721 tttacctgca aggtcttctg cttggtagtc ttctgggaat gttacgataa catcaacggt
     9781 ttcgccagct gagtgaccta ccaattggtc ttcgaaacca gggatgaatt gacctgaacc
     9841 aagtccaagt gagaagtttt caccttttcc accgtcaaat tcaacaccgt cgatagaacc
     9901 aacgaagtcg atcacaacag tgtcgccgtt ttcagcagca gcttccttga taaccaattc
     9961 agccaggttg ttgcgttcgc gttcgatacg ctcttcaaca tcagcgtcag ttacttcttt
    10021 ttctacatca actgatactt caaggttttt gtagtcaccc aattttactt caggttttgt
    10081 aacgacttca gcagcgataa cccagtcttg acctttttcc attgaagtta cgtcaatttt
    10141 tggttgggca accacttcaa gaccagcttc ttttacagct gcttcataag cgtttggcaa
    10201 aagagcgttc ataacgtctt ggtaaagtga ttcttcacca aattttttat cgaagatagg
    10261 acgtggaagg tgacctttac ggaaacctgg aacattaaga gatttcttca ctgacttgaa
    10321 gacacggtcc aattctggtt tgatttggtc ttgagagata gtgaaagtca agacaccacg
    10381 gtttgtttct ttgttttcaa atgatacaga cattctgtca tttctcctta aaatttttta
    10441 aatacagtct attataacat tttctataaa taaaatctag actaatgcag ccaattatag
    10501 acaacaagcc tttttcctaa ctaaaacact cataaagtat agacaatttt agactacttt
    10561 ggatatcttt tggttagtca ttttccaaaa ctaatttatc ta
//
LOCUS       DAVKZB010000060        10557 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKZB010000060 DAVKZB010000000
VERSION     DAVKZB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.060"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(56..286)
                     /locus_tag="VXA80_001359"
     CDS             complement(56..286)
                     /locus_tag="VXA80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV0353847.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGNKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VXA80_001360"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VXA80_001360"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0353848.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VXA80_001361"
     CDS             complement(1113..2036)
                     /locus_tag="VXA80_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV0353849.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VXA80_001362"
     CDS             complement(2033..2722)
                     /locus_tag="VXA80_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV0353850.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2787..3491)
                     /locus_tag="VXA80_001363"
     CDS             complement(2787..3491)
                     /locus_tag="VXA80_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353851.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3547..4569)
                     /locus_tag="VXA80_001364"
     CDS             complement(3547..4569)
                     /locus_tag="VXA80_001364"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV0353852.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VXA80_001365"
     CDS             complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VXA80_001365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV0353853.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSNYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VXA80_001366"
     CDS             complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VXA80_001366"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV0353854.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLVAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9669..10310
                     /gene="plsY"
                     /locus_tag="VXA80_001367"
     CDS             9669..10310
                     /gene="plsY"
                     /locus_tag="VXA80_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV0353855.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10355..>10557)
                     /locus_tag="VXA80_001368"
     CDS             complement(10355..>10557)
                     /locus_tag="VXA80_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEV0353856.1"
                     /translation="SPDNGMMESFFGILKSEMFYGYEKSFQSFKQLEQAIIDYIDYYN
                     NKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3031 a         2380 c         1867 g         3279 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt ttaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttgtt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaatcgatt tagcatagct aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctgatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caatcccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgctgccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggctg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca gtatttttaa
     2761 aattttatca agatcaatac cattccttat ttaatgtatt tttctagttc tttttggtat
     2821 ttgctattgg attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt
     2881 gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata
     2941 ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg aaaaccacca
     3001 gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat
     3061 ttttcatatc tcttctctag gtcagtggtc tcagaaacag catcttctaa caatttctta
     3121 tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt aatgccaagg
     3181 tgtttcatgg cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga
     3241 tagcttggag cccatcctgt actgttcaaa tcatagtctt tttgagaagg agcaacattg
     3301 ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat
     3361 gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat
     3421 gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg
     3481 ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg
     3541 tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat
     3601 acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa
     3661 tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc
     3721 acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg
     3781 agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa
     3841 gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa agaaattagc
     3901 tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa
     3961 atggcgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt
     4021 agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt
     4081 tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta
     4141 ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata
     4201 taaggttcca cctattccgt atggtggtac gtattcttca ttcgcacgga caactgcttt
     4261 acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg
     4321 ttgcaggcgt ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg
     4381 ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga
     4441 aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg
     4501 agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt
     4561 tactgtcata ttccactcct tattctaaaa acctatttct tatattctac actatttttc
     4621 taaaatagca agtatatttt gtaattttca gaaaattcct ccaataaaaa ccatcttaga
     4681 actgattctt catttcactt atttatcttc agtaactact tcctgaagat aagcgtcaaa
     4741 aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc gttctgacaa
     4801 gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga caagcagagt
     4861 ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt gctctgcaac
     4921 tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga cttgtaatcc
     4981 tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca agcttccacg
     5041 ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga ggacatcatc
     5101 ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg gaacctcttc
     5161 gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat ctagtttaat
     5221 cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact tgacagactt
     5281 agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga tttgaccaag
     5341 gcgagtcact gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt ccacataaag
     5401 gatttcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc cgatgtcctt
     5461 ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa gacttgtgaa
     5521 catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt catcatcacg
     5581 cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac gcttgatgta
     5641 acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag ctgtatcaat
     5701 ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa atttcttctt
     5761 gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac cgataatagc
     5821 cgccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga caaccacatc
     5881 ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt cttccgtaaa
     5941 atcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag cacggataag
     6001 agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga tatggagacg
     6061 tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc gacggtgagc
     6121 gatgtagtta gacaggattg gaacaatccc aacctgacga ggtgtgaaat tgtcaatcgc
     6181 caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt agttgagaac
     6241 aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat cacggtcaga
     6301 ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat catcgatttt
     6361 cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga tttgttcctt
     6421 accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc ctttcccagt
     6481 ctcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag ggaagtctgg
     6541 tccaggcaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa tcatgtaaac
     6601 tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat aaccagccga
     6661 aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga ccgttggttc
     6721 tttctccgta tcgtcaaagt tccatgcaaa aggaactgtc tttttctcga tatcctgaag
     6781 aaggtagcct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg caggaggatc
     6841 tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat ttttccagtt
     6901 ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt ggaaattccc
     6961 catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag tattgctatc
     7021 cttattcata gaataaagaa tacggcgttg aaccggcttc aacccatcac gaatatctgg
     7081 caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc ccatgatgtc
     7141 ctccagggac atgttttgaa tgttagacat aagatacaaa gcccataaaa taccaagtga
     7201 aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt cacacagtat
     7261 ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca gtaaaagata
     7321 ttttacggga attcgtcccg tgttcagtta cgataagtaa ccaaactatc ctgtttgtat
     7381 ttttcaatat gaaaatctgg ttttccaaaa ttagtcttag tttgtgtctt aaccgctccc
     7441 ttaagcgcct ctttaagata agcactcata gcagattctt cattaataat cctgcaattt
     7501 tttcaaacca agattttcaa actgcttttt cacatagtca ttcacatccg actctaattt
     7561 ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc tagcgtaaac
     7621 ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat gagaacattg
     7681 acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt acgtgtttct
     7741 gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc tttgtatcgt
     7801 tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc atccgtccaa
     7861 gcgtaggcca cttcttcttt cttgccttta cctttggaca tcttataaag aggtgggagg
     7921 gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa aaatgtcaag
     7981 agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat gatcttatca
     8041 tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt ataaatcatg
     8101 gtattgatct cttcattttt gaggatatcc gccatcttgg ccttggctgt attgacaacc
     8161 ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt ggcagaacca
     8221 ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt agattgggct
     8281 ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc atttcggctc
     8341 tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc cttgcggatg
     8401 aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc agccactatt
     8461 ccatccacaa ctgggcgagc tagggggctt cctagtttat ccttggtctg tccttcaaac
     8521 tgcaagtgtt cttcaggaac taagatagaa agaacggcca ctagtccctc acgatagtct
     8581 gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc atagtcattc
     8641 atgaccttgg taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc cttggtgcga
     8701 acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg gagggctact
     8761 tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa gatttcctta
     8821 tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa ctcaatcgct
     8881 tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa ggctgattca
     8941 ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaagat agtcgcgtca
     9001 ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat tttcttcaaa
     9061 gtcgtgacag gttttccacc attttcgaaa cgttgcttgt aaactgcgcc atcacgggta
     9121 atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac tccgtgaagt
     9181 ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag aatggtaaag
     9241 ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc acgtccatgg
     9301 tcttgaaccg ttagactacc gtctttattg atggttacat cgatacgatc accaaaccca
     9361 gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg aagaccagcg
     9421 ccatcggtcg atccaatata catccctgga cgttttcgga ccgcatccaa cccttctagc
     9481 acctgaatag catcatcatt ataattgtta atattgattt ccttttttga cacaaggaac
     9541 ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca aaatttttct
     9601 ttctccgatg tgacaatttt agcagagatt ctctgctttt ctttcccaat tcatgatata
     9661 ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt tcgattccat
     9721 ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat ggttctggta
     9781 acactggaac gaccaatacc ttccgcattt taggtaagaa agctggtatg gcaacctttg
     9841 tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt catctacaag
     9901 gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc cctatctttg
     9961 caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtaattttc ggatttgcgc
    10021 ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt ggcagtatga
    10081 tttcactgtc tagtgtcaca gcatcgatcg cggctgttat cggggttctg ctctttccac
    10141 tttttggttt tatcctgagt aactatgact ctctcttcat cgctattatc ttagcacttg
    10201 ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat aaaactgaaa
    10261 atttggtccc ttggggattg aacttaaccc atcaagatcc taaaaaataa aatgccagtt
    10321 ctgtgaactg caccccaaaa gttagacaat taatttatcc gaaggattta gttctgtatt
    10381 gcacagggct aagtcctttt agttttacct taattcgttt attgttgtag taatcaatat
    10441 agtctataat agcttgttcc aattgcttaa acgactgaaa cgacttctca taaccgtaaa
    10501 acatttccga tttcagaatc ccaaaaaagg actccatcat accattgtct gggctgt
//
LOCUS       DAVKZB010000061        10547 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKZB010000061 DAVKZB010000000
VERSION     DAVKZB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.061"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(66..1490)
                     /locus_tag="VXA80_001369"
     CDS             complement(66..1490)
                     /locus_tag="VXA80_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0353857.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2033..4312)
                     /gene="pulA"
                     /locus_tag="VXA80_001370"
     CDS             complement(2033..4312)
                     /gene="pulA"
                     /locus_tag="VXA80_001370"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV0353858.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKIETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTSAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4424..6382)
                     /gene="ligA"
                     /locus_tag="VXA80_001371"
     CDS             complement(4424..6382)
                     /gene="ligA"
                     /locus_tag="VXA80_001371"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV0353859.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLENLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6475..7653)
                     /locus_tag="VXA80_001372"
     CDS             complement(6475..7653)
                     /locus_tag="VXA80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0353860.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLVLTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7733..8596)
                     /locus_tag="VXA80_001373"
     CDS             complement(7733..8596)
                     /locus_tag="VXA80_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0353861.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     AKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFDN
                     LYSFYDVDYITRIGREVMEREEFYQEIGRHKRLVLILALNCYQHCLDHLSFDNASYFE
                     AYTEKIIGKGIKLYERNAFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8599..10500)
                     /locus_tag="VXA80_001374"
     CDS             complement(8599..10500)
                     /locus_tag="VXA80_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0353862.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTASNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSALYLGDYDYYIEKKVEVEMSQIEEASTNNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLETEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3164 a         2345 c         1886 g         3152 t
ORIGIN      
        1 ctataattat tattatttta tagcaaaaaa ataaaaccag gaaaacaatt tcctgatttt
       61 acacgttatt tcacatcaaa aacaatggat ttgacatttg tcatcgcttc aatgctatat
      121 ttaattcctt gcactccagc tccagaacct ttgacaccaa ggaatgggaa attatctgga
      181 ccacgctggg ttttattatt aatgtggact gtacctactt caagtttttc agcaatttca
      241 aaggcttttt tgaaatcatt tgtaaagact gatgattgaa ggccgaattc agattcgttg
      301 gcaaaggcaa tagcttcctc tacactagcc acacgaatga ttggtaaaac aggaccaaat
      361 ggctcttccc atgccacttt catatctttt gtaacttggt caaaaagcac tggccagagg
      421 agattgccct cacgtttgat tggtgtaaga gcctgagcct ctttttcttg tgcatcctca
      481 atcaagcccc aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat atcagcattg
      541 tcaaatggat caccaactgt taatttagaa acttcttcct gaagcaaagt agctaattta
      601 tctgctacac tttcgagaac aatgacacgt ttaatagccg tgcaacgttg tcctgagtag
      661 ctaaaggctc ctgcaacaat ttgcttggca gcatgttcca aatctgcatc ttctagtaca
      721 agagctgcat ctttcccacc aagttccaac atgataggac gcataccagc taaacgacca
      781 atacgttctc caataggagt tgaacctgta aagttgatga agttgacttc tttgtgctca
      841 atgatataat ccccaatttc tgaaccacga cctgtaatgg tgttgaaaac acctgccgga
      901 atccctgctt cttcaaatgc tttagccaac aagagtccag aaatggaacc ttgtgttggt
      961 ggcttaaaca tgaccacatt ccctgcaatc aaggcaggtg caattttaga agcagataaa
     1021 ttaactggat aattaaaagg agcaatcgct agcacgatac caactggttc acgacggaca
     1081 acagccagtt tgtttttact tgctgcctca aaaccaccac cttccattgc ttgtccagtg
     1141 atacggagac cttcctcagc agcataacga atcaagtctg ctgtacgcac tacttctcca
     1201 attgctgctt taatcccttt tgctacttct ttggcaagga tagtaccaat ttcttcctta
     1261 tcgcgttcta aaatagctgc tgttttatgc aaataagccg cacgttcaac tgctgataaa
     1321 gctcgccatg ctggcagggc tgcacgcgca gcttgcatag cctcatcagc ttcagtctga
     1381 gtcatggctg gaactgtacc caattcttct tgattgattg gtgaataaat cgtaatttct
     1441 tgttcagatg atttccattt tccatttact aaattctgat atcttgtcaa atttttgtcc
     1501 tcctgaaaat gattaagata tattttagca aattttcaga aaatcacaac cctttttgca
     1561 aaatttttga gattattttc acaagcttga tttttcaaag tatactcaat aaaaataaaa
     1621 aaatccacta cgtcaaggcg agactaatgt ggtttgaaga aattttcgaa gagcgtgaat
     1681 gagtatcatc tatagtaaaa taaaaaactg aacaatttgg ttggggacag ccaaaccaat
     1741 ttctcacaat gttttagaaa caagggtgtg ctattccaat ttcagcctac tataactgtc
     1801 atagattgtt gaaacaaagt ctaggtaaaa gtcttcataa taaaaagacc tcctatcaaa
     1861 tgttcaaaaa ctttgatagg aggtcttgtt tcgttaaaat atttatcaaa ttttctatac
     1921 aagtgagctg ttagccaggt tctttctatt ctttcaattt caatgaatgg attttttatc
     1981 aatactcata actgggaatt tgtctgtgta aaaatagcga gatagatggt atttataaaa
     2041 cactcaagac agctagacta atatcattta aaacattatc ttcttttgag cgactgttgg
     2101 ttaccaacat agctaaattt cctgcatttt caaattgata gggttctgat ttagcattca
     2161 caaccaccaa gaggtgttct ttgccgtgaa cttcatagat aaggcagccg ctatgttcaa
     2221 tcgcagaatg cacaaagaca tgatggtaaa tttcatcata gctagagtaa gaaaaggcac
     2281 tagtttttgt cttcaatcgg atgacttgac ggataaactc aatactgtct tgacgctcat
     2341 taatcaagtt ccagttcact tggttcacac tgtcaggagc attatagcta ttcatcgcac
     2401 gctctctatc atcatgggtc aactcaccat tttcaccagt cgcaaccagt ttggtacgac
     2461 caaattcttg accgatttcc ataaaggcca tcccctgcat gagcagattc atggctgtgg
     2521 cagtttcgat cttgcgcatg atttgctctg aactttggtc tggatgaagg gttgccaata
     2581 aatcgtgaag attgtaattg tcatgggctt ctacatagtt aagcacctga tttggatgtg
     2641 tatagcttcc taattcacga cttcctagga ttgctttagc tagaattggc tctgtcgcag
     2701 caccactgac aaaacctgac ttgatagcac cataaacttc tccccctttg acagcatcgc
     2761 gctgattgtc attgaagaaa ccaatatttg gcatctggta ggcattgtcc ttcttggcct
     2821 tatcataagg ggtaagacct gttcccatat cccatccttc tccatagagg ataatgttgg
     2881 agtcgatttc atccaagctt tgacgaatca tctgcatggt cttgacatca tgaatcccca
     2941 tcaagtcaaa acggaagccg tcaatattat attcctgcac ccagtataga agagaatcaa
     3001 tcatatactt gcgaaacatt tcgtgttcac tggctgtttc atttccaaca cccgttccat
     3061 tctggaaggt accatctgga ttcatacgat aatagtaatc agggactgtt gtttggaatg
     3121 gtgcatcaac aactgagaag gtatggttat agactacatc cataatgact ccaatacccg
     3181 catcgtgata agcttgaacc atcaccttca aatcacgaat gacctgagct ggatcatctg
     3241 gattagttga aaaactagtt tctggcgcgt tatagttttg tggatcataa ccccagttgt
     3301 aggttacatt tccatcctca tcgtattctt tatgacggtc tgcaattggt tgcaattgaa
     3361 cataattgta gcccagcttc ttgatgtaat caaaagcagt tgactggccg tattggttaa
     3421 ctgttccagc ctgagcagca cccaagaaag ttcctcgaag atgttcatct acacccgatg
     3481 taggtgattt agtcaaatca cgaatatgca tttcacagat aactgcctta catggatttt
     3541 ccaagcgcca agtagcctcc gaaccgtgct taacctcgaa gttttcaact tgcttttcta
     3601 catggctcag aatagctgaa cgtttgccat cagggctggt cgcgattgta taaggatcac
     3661 gtgtcagtgt ttggtgatga gggaattgga cttgatactg ataagcctta cctaccaaat
     3721 cttcctcaac atccaaactc cagacaccga ttgtattgtc cttatgatta taagagtagc
     3781 tattgcctct tttcatctca aaagtcttcc aaacgggtgc atcattagca gctgattcat
     3841 aaacgacaac ttgcacttct gtcgctgtag gtgaccagag ggaaaaatga gcctgattgt
     3901 cctctacacg gcaacccaat tctccttggt aaccccaatg atgatcaaaa ctagcactgt
     3961 taatggcctt atcaaaggca aaaggatttt gatttttata gaaaggactg gcaatagcag
     4021 gattttcaga gtaataaatc ctatcatcgc cttccaaaat ccagacctct gttaataggg
     4081 gatagtgatt aaaacggata gaatattctt tactagtttg acctgtatga accacaaaat
     4141 tcaagctttc tataacatgt gaacttgggt gttcaaagct aaataaagct ccaaaataat
     4201 cttctttgta ggttagcaaa tcaattcgtt gatcctgact ttttacaaag gagcaagtgt
     4261 catattctcc attcttacga tggtaatgaa tgcgcatagg gtagttatac attttttatt
     4321 tttccttttt actttgtttc tatttcacta ataaattttt gtcaatctcg tctcaattaa
     4381 cagacatagt catattctct aaactctgtt tttaaacaat ccattacaaa ctttctagcc
     4441 atgcctcatc tctgacctgg ataccaagtt cttgtgcttt ttgcagttta cttccagcgt
     4501 ctgcacctac cacgacgagg tcggtctttt tagaaacact acctgtcact ttggcaccca
     4561 gattttcgag tttactttta gcttctgagc gcttgagtcg ttccaatttt cctgtcaata
     4621 ccacggtcaa acctgacaag gccgcatccg ctactaccgt ctgtccttta tagtccagat
     4681 tgaccccagt ttctttcaat tctctgagca gaatttcaga gccttctgtc gcaaaataag
     4741 tctgaagact tttggcaatc acgccaccta gactttcaat actagccact tcctctgaat
     4801 ctgcctgata cagattttca attgaatgga aatattgaag taaaagctga ctagccttgc
     4861 ttccgacatg acgaattccc aaaccaaata agagcttctc ggcagaattt tcctttgatg
     4921 cttggatagc ctgatacagt ttagcagcgg acttttcctt aaccccctct aaaaggagga
     4981 aatcctcttc ttgcaaacga taaatatccg ccacatcctt gactaaatta gcagcaaaaa
     5041 gcttctcaac aatagatgga ccaaggcctg taatattcat agcatcacga gaagcaaagt
     5101 gaatcaagcc ttccatgatt tgagcagggc aacgcggatt gatacaacgt agggccactt
     5161 catcttcaaa gtgcaacaag tcagagttac aacttggaca gtttgtaggg atatctagtt
     5221 tttcttcaga aacccgtttg gactctacca cacgtaaaac ggcagggatg atgtcaccag
     5281 ccttatatac aatgaccgta tcgtcttttc ggatatcttt ttcagcaata taatctacat
     5341 tgtgcagggt cgcacggcta acagtcgtac cggcaagttg tactggtgtt agattagcag
     5401 ttggagttac aacaccggta cggccaactg tccagtcaac tgataagagt tgagcttctt
     5461 tttcttcagc agggaacttg taggctactg cccactttgg agccttaacg gtaaaaccaa
     5521 gttcttcttg acttgctagg tcgttgacct tgattaccac tccatcaata tcgtaaggca
     5581 gattttcccg ttcctgtcct acttcttgga taaaattcca gatttcatct atgttttcag
     5641 ccaagattcg cttaggattg accacaaaac ctagttgttc taggtacttc aaaccctttt
     5701 cttggctatc acgagttgaa gggctggctt cttgatagag aaacgttgca agattacgct
     5761 tggcaactac tgctgtatcc aactgacgca gagttcctgc tgccgcatta cgaggattag
     5821 caaattcagg ctctccattt tcttggcgca cttggttaac ttggtcaaag gaagcgcgtg
     5881 gcatgtaaca ttccccacga actgtgatat ctagttcttc tggcaaagtc aaagggatgt
     5941 ccttaacacg cttgaggttt tctgtgatat tttcaccaat tgaaccatct ccacgtgtta
     6001 ccccagcaac caaaatcccc ttttcataag tcagcgagat agataagcca tcgattttca
     6061 gctcacaaat ataggtcgga tgagccactt ccttacgaac acgcgcatca aaagcatcta
     6121 gctcctcacg tgaaaaagca tcctgcaaac tataaagagg atactgatga ctgtattttt
     6181 caaaaccatc taaaaccttg ccaccaacac gatgagtcgg actgtctgct agcacttgct
     6241 ctggataagc agtttctaac tcgaccaact cacggtaaag gcggtcatac tcactgtctg
     6301 aaaccgaggg attatcgctg gtatagtact cagtcgcata gcgattgagc aaagcgacta
     6361 actcattcat tcttttattc ataagaccat tttaccataa aacaagccct cctcacaaac
     6421 gagaagggcg gaaaaaacac ttagtttgaa attatttttg aaactcaagc aaccttatat
     6481 caatttttca aaatgagttc gaacataaat aaacgatata caagacaaga tgataacacc
     6541 acttccaatt atcaggaaag aagagagatg tacacttggc aagactgtca taaatccttt
     6601 tgcaataggc ataaatagaa tagctaaggt aaaaattgta ctcagtactc tcccaagaaa
     6661 ttcgctctca accttggttt gtacttgagt aaaaaagtga atattaaaaa tcgtcataaa
     6721 caattcacaa actaaatttc cagaaaagga aagaaaagtt ggaagtggta atcccatcat
     6781 aaaaactccg acacctgtca aaaccagtaa aatcaaaaga ttataaatat tagctttaat
     6841 tttactagct agaagagccc caatgatgga accaatagcc cccatagtta aaatacttgc
     6901 ataggctcct tctgacccgt aaagctgatt cgaaaaggga agtagaaatt caaaagctgc
     6961 aaaaaagaaa ttaacgctgg aagctaccag caaaaggaag aaaatttctt gctgatgcca
     7021 gatatagtgt aacccatcct tgatatctac aaaaatatct ctcccagtaa aagccttttt
     7081 ctcttgaact tttgcttcct cttttggaag gaaagccact agaacaaaag caatgaaaaa
     7141 agtcagcgag tctagcagta gcgtcatatg gagacttgca aactgtaaaa caaggaagga
     7201 aagaacagga gagctaacac ctacaacctg caaaaccagc tctaagcgag aattatagat
     7261 cacaatctca tctttctcca ccacttcagt tatgatagct ttattggctg tgcgagaaaa
     7321 ggcaaaagca atagcctgca caatgttagc aacaatcaaa gcgccaatca tccagctatc
     7381 attccttatg aaagaaatag ccagacaaag aatcccacaa acaagatctg ccgtcattaa
     7441 aatcttacga cgagaaaaac ggtctgaaat aactccgcca aagggattga cgagaataga
     7501 tgtgacgagc tcagaaatct gatacattcc taaaactgtc tgtcctatag tccccataga
     7561 agccaaccag acactatttc cataatcata gagcatattt cccattttat tgatagcccc
     7621 acggctaatc aactgcactg catagcgatt catattaaag ctcctctcaa attttgaaac
     7681 tattgtatca aaaccgaaag gagcttttta ttttttccct tatttgggaa aattaacttt
     7741 tgacaaattt ttcgtagtgt tcctgataat aggctacttg ctctggaaga cctaacacat
     7801 caaaaatatg catggcctct tgcatctgct tacagccttc tttacactgt cctttttgat
     7861 ataaggcaaa accttttaaa taatggaaag cattacgctc ataaagcttg atcccttttc
     7921 caataatctt ctctgtataa gcctcaaaat agcttgcatt gtcaaaagaa agatgatcta
     7981 aacaatgctg gtaacaattg agggccaaaa tcaacactaa tctcttatgg cgaccaatct
     8041 cttggtaaaa ttcctccctc tccataactt ctctaccaat ccgagtgata taatccacat
     8101 cgtagaaact atagaggtta tcgaaaagaa tcaactcata catggtccat tcttctgttt
     8161 tgaagagata atctgctacc ttatccaaat catcctgctt catatcataa gtcgcatctc
     8221 tttgacaaat cagaccctgt agcaaaatcc agttcagctc aaaataaagg ggagtcgtcg
     8281 aactcttagc cttttcaagt tgttctcttt gaagcttttg aaaacctgca atatcgtttg
     8341 aatagtaaag tgggataatc tgtgccatca tagacacatg ttcatgatta tgaaaattcc
     8401 ttgccttatc catgaaattt tcgattgtga catgaatgtt atccaatatc tcaaaaaaac
     8461 gggagactgc taggtcagac tccccaagct caaagcgaga taactgagag gttgaacagg
     8521 attcccctgc tgcctccttt aaggaataat ttccacttgt tcgaaattca cgaaatactt
     8581 ttccaagatg ttccatcttt acacctgctc tgataattct tcccattcaa gcatagcttc
     8641 ttcctgacga tggctgattt tgtccagctc agcctgcaat tccatgagtt tatcggcatc
     8701 gtttgtttcc aacatttgtt cagaaatggc ttgactttga ctttctaact cttcgatttc
     8761 agtttctaga ctttcaattt gtcgcatgag tttgcgaact tctttttgac tttctttctg
     8821 ggcctgataa tcgttgactg gacttgcttc ctttgcttga ttgttagttg aagcttcctc
     8881 aatctgactc atttctactt ctactttctt ctcaatatag taatcgtagt ctccaaggta
     8941 gagagccgag ccattctcag acaattccaa aacatgagtt gccacacgat tgataaagta
     9001 acggtcgtga ctgacaaaaa gcaaggtacc gtcaaagtca atcaaagcat tttccaacac
     9061 ttccttgcta tcaatatcca agtggttggt cggctcgtcc agaatcaaga agttgttgtt
     9121 ttccatagat aatttagcca aaagcaaacg tgctttctcg ccaccagaca gcatgccgac
     9181 tgatttttta acatcatcac cagagaaaag gaaggcacct agacgattac ggatttcaac
     9241 ttccggtgtc agtttaaaat cattccaaag ttcatctagg acggtattac tcgctgtcag
     9301 cttgctttga gtctgatcat agtagccaac ctcaacatta gcgccaaagc gcttctcacc
     9361 cttgataaaa ggaatctggt ctactataga cttgataaag gttgattttc cgataccatt
     9421 gggaccaacg atagcaacag cattcatctt acgaaggtct aggttaatcg gttgtgacag
     9481 gacttcccca tcatagccaa cagccgcatt ttcaacagtc aaaacaacat tgcccgacgt
     9541 tttttcagac tggaaggtca tgttggctgc tttcttgcca gcttccggct tgtccaaacg
     9601 ctccattttt tctagttgtt tacggcgaga ttgagcacgt ttggtcgttg aagcacgaac
     9661 tagattgcga ttgacaaagt cttccagagc agcgatttcc ttctgttgct tttcatagtt
     9721 ttttgcctca gtagctagct tttgctcctt caactcgaca aaacgagagt aattccccac
     9781 atagcgatcc aaggaatgct tggtcaaatc tagcgtaatt gtcgcaacct tgtccaagaa
     9841 ataacggtcg tggctgacga taatgagggc accgctatag tttaccaagt aattctctag
     9901 ccaggagatg gtttcaatat ccaagtggtt agttggctcg tccaagacca agagattggg
     9961 cttttcaagg agcattttag ctagagccaa acgagtattt tgaccacctg aaagttcagc
    10021 tattttcatc tgccacatag actcgtcaaa cttgaatcca ttcaaaatcg ctcgaatatc
    10081 agcttcatag gtaaagccgc ctgcttggcg aaaattctca gataagtggt cgtaatctga
    10141 catcagttta tccaaatcct caccagactt ttcacccatc tccaactcca tctgacgaag
    10201 ttgtttatct gtccgacgta agtcatcaaa gacatgaagc atctcatcat agatggtatt
    10261 ttcagactca aaacggctat cttgagctag gtaagacaga gaaatatctt ttttcttatt
    10321 gatttctcca ctagttggct cctcttctcc aactaaaatc ttcaaaagag tagacttacc
    10381 tgcaccattt ttcccaacaa gggcaatccg atctcgttca tcaacctgca ggttgatatt
    10441 atcgaaaaga acctctcctg caaaagaacg ttcaatttta ttagcttgta aaataatcat
    10501 acaagtagta tagcatgttt ccctaagtca ttcaagataa tcgtaag
//
LOCUS       DAVKZB010000062        10513 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKZB010000062 DAVKZB010000000
VERSION     DAVKZB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.062"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..466
                     /locus_tag="VXA80_001375"
                     /pseudo
     CDS             1..466
                     /locus_tag="VXA80_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            432..812
                     /locus_tag="VXA80_001376"
     CDS             432..812
                     /locus_tag="VXA80_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0353863.1"
                     /translation="MRLFCLVLVSIDYINCLSETTDKNWHKSDRVFVTNTGKTVHSSI
                     LSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTSI
                     YTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(950..2254)
                     /gene="eno"
                     /locus_tag="VXA80_001377"
     CDS             complement(950..2254)
                     /gene="eno"
                     /locus_tag="VXA80_001377"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV0353864.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2418..2864
                     /locus_tag="VXA80_001378"
     CDS             2418..2864
                     /locus_tag="VXA80_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV0353865.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKIWEVKKEEPAKTSLWKKWFS"
     gene            complement(2861..3976)
                     /locus_tag="VXA80_001379"
     CDS             complement(2861..3976)
                     /locus_tag="VXA80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV0353866.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4090..4314)
                     /locus_tag="VXA80_001380"
     CDS             complement(4090..4314)
                     /locus_tag="VXA80_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0353867.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            complement(4259..4630)
                     /locus_tag="VXA80_001381"
     CDS             complement(4259..4630)
                     /locus_tag="VXA80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV0353868.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4689..6122)
                     /gene="glgA"
                     /locus_tag="VXA80_001382"
     CDS             complement(4689..6122)
                     /gene="glgA"
                     /locus_tag="VXA80_001382"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV0353869.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQTYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6119..7258)
                     /gene="glgD"
                     /locus_tag="VXA80_001383"
     CDS             complement(6119..7258)
                     /gene="glgD"
                     /locus_tag="VXA80_001383"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV0353870.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7248..8390)
                     /locus_tag="VXA80_001384"
     CDS             complement(7248..8390)
                     /locus_tag="VXA80_001384"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV0353871.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8380..10308)
                     /gene="glgB"
                     /locus_tag="VXA80_001385"
     CDS             complement(8380..10308)
                     /gene="glgB"
                     /locus_tag="VXA80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV0353872.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3193 a         2306 c         1865 g         3149 t
ORIGIN      
        1 gcacctagca cctgtttaga ttgacgaaac aggattcgat acttattttt atcgagaata
       61 tggtcgctca ttaaaaggtc agttaataag aggcaaagta tctggaagaa gatatcagag
      121 gatttctttg gttgcaggtc taacaaatgg tgaattaatc gctccaatga cttacgaaga
      181 gacgatgacg agcgactttt ttgaagcttg gtttcagaag tttctcttac caacattaac
      241 cacaccatcg gttattatta tggataatgc aagattccat agaatgggga agctagaact
      301 cttgtgtgaa gagtttgggc ataaattttt acctcttcct ccctactcac ctgagcacaa
      361 tcctattgag aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat taccaagttg
      421 caataccttt tatgaggctc ttttgtcttg ttcttgtttc aattgactat ataaattgtc
      481 taagcgaaac aaccgataag aattggcaca aaagcgaccg tgtttttgtt accaatacag
      541 gaaaaacagt tcatagttct atcttgagca agtctctcca gcgagcaaac gaacgcctta
      601 aaaaaccaat tcccaaacat ctgtcccctc acatcttcag acacaccact attagcatct
      661 tatcagaaaa taaaattcct ttaaaaacaa tcacggacag ggttggtcat tccgactctg
      721 aagtcactac ttccatctac acccacgtca caaagaacat gaaagatgaa gcaatcaatg
      781 tactggataa agttatgaaa aagatttttt aaaaagtttt gccccttttt tgtcctctaa
      841 atacaaaaat agcccttcgg ataaaatccg aggggctaga aacgttgtta aatcaacggc
      901 cgaacttttg aatttcatgg ttcgggataa aatagttcac tgaactattt tattttttaa
      961 ggttgtagaa tgatttcaat ccacgatatt cagctacttc accaagttgg tcttcgatac
     1021 gaagcaattg gttgtattta gcgatgcggt ctgtacgtga aagtgaacca gtcttgattt
     1081 gtcctgcgtt agttgcaact gcgatatcag cgattgttga atcttcagtt tcacctgaac
     1141 ggtgtgatac aacagcagtg taaccagctt ctttagccat ttcgatagct tcaaaagttt
     1201 cagtaagagt accgatttgg ttaactttga taaggattga gttagcagca ccttcttgga
     1261 taccacgtgc aaggtagtca gtgtttgtta cgaagaagtc gtcaccaaca agttgtactt
     1321 tcttaccaag acgttcagta agagctttcc aaccatccca gtcgttttca tccataccat
     1381 cttcaatagt gatgattggg tatttgttaa ccaattcttc aaggtagtcg atttgttctg
     1441 cagatgtacg aacagcagca ccttcacctt caaatttagt gtagtcgtaa actttacgtt
     1501 ctttatcgta gaattctgat gaagcacagt caaatccgat aaatacgtct ttacctggta
     1561 catatccagc agcttcaatc gcagcaagga tagtttcaac accatcttca gttccttcga
     1621 aacgaggagc aaatccacct tcgtcaccta cggcagtttc caaaccacgt gatttaagga
     1681 ttttcttaag agcgtggaag atttcagcac cgtaacgaag ggcttcttta aatgttggcg
     1741 caccaactgg caagatcatg aactcttgga aagcgattgg agcgtcagag tgagaaccac
     1801 cgttgatgat gttcatcatt ggagttggaa gaactttagt gttgaatcca ccaagatagc
     1861 tgtaaagtgg gatttcaagg tagtcagcag cagcacgagc tacagcgata gacacaccga
     1921 ggattgcatt cgcacccaat ttacctttgt taggagtacc gtcaagtgcg atcatagcac
     1981 ggtcaatagc ttgttgatca cgtacatcgt agccaatgat agcttcagca atgatgttgt
     2041 ttacgttgtc aacagctttt tgtgtaccaa gaccaccgta acgagatttg tcaccgtcgc
     2101 gaagttcaac tgcttcgtgt tcaccagtag aagctcctga tggaaccata ccacgtccga
     2161 aagcacctga ttcagtgtaa acttctactt caagtgttgg gttaccgcgt gagtctagga
     2221 cttcgcgagc gtaaacatca gtaataattg acatttttta ctctccttat gagttaaatt
     2281 ttttacacct ctataatacc ttaaaacccc tcctttttca agaaaaaacg ttatctttgt
     2341 gcaacttttc cttaacttta taaagtaatc gctttctttt gtctgtttta ttctaacttt
     2401 tatgatatac tgttttcatg acagatttat caaaacaatt acttgaaaaa gctcatggtg
     2461 ggttaaaaat aaatccggat gagcaaagac gctatcttgg tacttttgag gaaagagttc
     2521 ttggatatgt agatattgac acagcaaata gccctcagtt agaaaaaggc tttttattta
     2581 ttttagaaaa ccttcaggaa aaagcagagc cactatttgt gaagatttca ccaactatcg
     2641 aatttgataa gcaagttttc tacttaaaag aagcaaaaga aactgatagt caagccacca
     2701 tagtatctga agagcatatt acttctcctt tcggcctggt tattcatagc aatgcaccag
     2761 ttcaagtaga agaaaaagac cttcgacttg cttttccaaa aatttgggaa gttaaaaagg
     2821 aagaaccagc caaaacatcc ttatggaaga aatggtttag ctaaatcttg cacatattta
     2881 ataagtgccc aatattggca gccgtgcgct ccagatagag actggcattt ttcaaactat
     2941 cttctaaagg ttcacttttc tccaaaatag aaaagacagc ttggatattt tcaaatggta
     3001 ggggaggtaa atcttcagca agactaccgc aaatagcaat aacaggaact ccaacagggg
     3061 ttctttttgc aacacctata ggcgctttcc cagcaaagct ttgactatca agtcttcctt
     3121 ctccaacaac aaccaagtca gcatctgaaa ctttcttatc aaagttgatt aagtccaagc
     3181 aggtatcaat tccagacacg atacttgcct gagcaaaggc acacaaacca ccagcaaggc
     3241 ctccacctgc tcctgctcct ttaatttcta atgttgcagg tgagaatttt tcataaaaat
     3301 cttggatcgc ctgatctacg actgcaaaca tagtcggatg tagacctttt tgattgccaa
     3361 aagtgtaagt cgcaccttga tgaccacata agggactcac gacatctgct agaatatgaa
     3421 tttgaacacc ttcaggaatt ttatagcaat tttctgttga aacagaagct aagtttaata
     3481 aggattgacc ggaagcaggc aagacatttc catccctatc ataaaattga taacctaaac
     3541 cagcagcaat ccccagtcct ccatcattac tggccgtgcc accaacaccg atataaatat
     3601 ctttaatccc tttagaaatg agatggagaa tcaactctcc aataccacaa gtttggattt
     3661 gaagtggatt tcgtttctct agcggaattt ttccaagacc aaccaagtca gctacttcaa
     3721 atagtgccag ttcccctttt tgaaaatagc gcatggcttc tttttgtcca aaagggtctg
     3781 tcacttggat ccatttttct tttaggtcaa gagaatgtcg gatagcatct acagtacctt
     3841 ctcccccatc accaacaggg cagaggagac attctacatc tgctatcgat tgttggaagc
     3901 ctctttttat tgcttcagct acctgttgag ctgtcaagct ttccttaaac gaatccggtg
     3961 caattacaat cttcatattt tccctcattc taaacagtca atcaaaggaa gaacttctaa
     4021 aaaatccctc ttgtcaacat gatgtggtat ttcttttttg agcatttctt tggaacaaaa
     4081 ggcgattcct cactccgccg acttcaacat taatagatta ttgaccccat caccgattgc
     4141 caccgttctt tctttagaaa gttttagttt ctttctccat ttttccagag tttctttttt
     4201 gacctgggga cttataattt gtccaactaa ttttcctgtt aaaagacctt ctttgacttc
     4261 aagctggttg gcagtgaaat aggcaatacc aagggatttt gctaatctct ccaactattg
     4321 gtgtaaatcc accagacacc agaccaacta ggatgccatt cttttggaga atagagatga
     4381 actctgggac atttagcgat agatgaattg agttgaagac gttatcaaag accaaaatag
     4441 gaagaccttc caacaaggac actctttttc ttaaactgct ttcaaagacc aactctcctc
     4501 gcattgctcg acttgtaatc tgcgaaattt ctgcctcatg acctgcctct ctccctaaaa
     4561 gatcaatcac ttcttctagg attaaggttc catctacatc caaaacacac aagcctttta
     4621 cttgagacat cagttctcct ctctaaacag cctaaaaatc gtatgaagtc atcatacgat
     4681 tttatctatt aattaactaa actatggtac aagtcaaggt atgacttgca ggctgtatcc
     4741 catgagaagt cactctccat agcttgtttt tgtaggtttc tccaaatgtc tggatggttt
     4801 ctatacaagt ccaatgctgt ttggaaagtc caatttaacc aataaggaga tagattgtca
     4861 aagctaaagc cagtaccgct tccttcgatt ggattgaaag cgcgaactgt atctcgcaag
     4921 cctccaactt catggaccaa tggcaaggtt ccataacgca tagccatcat ttgagacaag
     4981 ccacacggtt caaaacgact tggcatgagg aagaggtcac aagcagcgta gatttcctga
     5041 gcaagtttga catcaaaagt gatatttgtt gatagcttgt ctgggtaaat ctgagcaaac
     5101 catgagaaag ctccttcaaa ggctggatcg ccagttccca aaagaacaat ctgaacatct
     5161 tcttgcaaga tatggtgaag actttcgacc accacatcaa aacctttttg acgtgtcaaa
     5221 cgagaaacaa ttcccaccag tggaacgtct gctctaacag gcaagccaac tctttcttgc
     5281 aattttgcct tatttttggc tttcccagac aaatcttcct gattgaaatg atagtctaaa
     5341 agagcatccg tctgaggatt ataaagatca gcatcaatcc cattcacgat accagatact
     5401 ttaccagact ccattttaag aatctgatcc aaattacatc caaactgact agtcataatt
     5461 tcatgagcat agctaggtga aacagttgaa acacggttcg catagagaat acctgccttc
     5521 atccagttca gacagttgtt ccatcgaagg gtgccatcag cgtaacgttc aaagccaact
     5581 ccaaacaaat cacccaacat tccttctgaa aattgtcctt ggaattctaa attatgaatg
     5641 gttaaaactg tttcaatgtc ctcataggtt tgaatccaac ggtatttttc cttcaacaag
     5701 aaaggaatca tagctgtatg gtagtcatga acatggagaa gatcaggaat aaagtcaatc
     5761 ctttccatag cctcaatggc agccagttgg aaaaaggcaa agcgttctcc gtcatcaaaa
     5821 tcaccgtaaa catgaccacg gaagaaataa tattgattgt caataaagta gaaggttaca
     5881 ccatttaata ctgttttctt aattccacaa tactgtctgc gccaaccaac gctcacctca
     5941 aaatgaagca catcttcaat ctgacttcca aatttagcct ctaccatatc atagtagggt
     6001 aaaatcactg caacttcgtg cccagctttt accagtgatt ttggaagagc gccaatgacg
     6061 tctcccaaac cacctgtttt tgaaaagggt gcaccctctg ctgctacaaa taaaattttc
     6121 atgaatgaat atcctctgtt actttagcac ctttcttaac cacaactgga tgttctgcag
     6181 ttcctcgaat cacaacacca ggctcaactt caaccccttt gtccaagata gcatattcga
     6241 cctgagcccc ttctccaata acaacacgag ggaagagcag gctatcttta accaagctat
     6301 ccttatggac atgaatatta cgtgatagaa cagaattagc tacttgacct tcaataatac
     6361 taccagaggc aaactgagaa gtgcttacct tagatgtatt agcatagtaa gttggctctt
     6421 cgtttttgac ctttgtataa atcttttggt ttggtgagaa aagagaatag aatttttgtg
     6481 attcaagcat atcgatattc gcttgataat aagatttaac agagtgaata ttggctagat
     6541 agcccgtgta ctcgtaggcg aaagctccct cttttacagc caaatcccgt aaaacatagc
     6601 gcaatttctc tggatgttct tttttagctt cttcttccaa gtgttcaatc aaccaaggtg
     6661 tatcaacgac aaagatatct gtagacatat tgaacgtttc agctgttgac ttgctatcaa
     6721 agagtttatg agaaagaaca tggtctgttt catctacatc caagattgca tttacttctg
     6781 aaatatcttt cttagctagt tttttataaa ctacagtgat aggctctttt gttgtactat
     6841 gtaggtggaa aacttggttc aaatcaatgt taataagaac atcgcagttg agggcaaccg
     6901 tttggtttga gccagaacgt ttcaaataag taagaagctg ttggtagtat tcttttccaa
     6961 ctgtactact ttctacacgg gtattgtaaa ttcctagata gtaatggcta agaagggttg
     7021 ataagcccca ctcgcgtcct gaacgaatat ggtcaaatac tgagctgata ttatcctgct
     7081 ggaaaatacc aaagacacta cgaacacctg cattagcaag gcttgaaagt gggaagtcaa
     7141 tcaaacgata tttcccacca aatggcaaac ttgctactgg acggtggtcc gtcaatgtcg
     7201 acatattgtg aaaaccaact gtatttccta aaatggcaga atatttatca atcttcatct
     7261 gttgctaccc ccactacttc attatatcct acaacttgta cttcatctgt tccatcaatt
     7321 tcgacaccgt cagaaataat cgcaccttca ccaataatgg cacgtttaat cttagctcct
     7381 tgaccaatga tagctccact catgataact gaatcaagga cttccgctcc ttcgcgaact
     7441 tgcgcgcctg ttgaaaggat agaatgttta acagttccat caacgaaaca tccgtctaca
     7501 actaatgagt cttccacatg agcatttgcc ccgaggaagt ttggtggtga aatcaagttt
     7561 cttgagtaaa tcttccattg acggttacga ctatccaagg cattttctgg agaaatatac
     7621 tccatgttcg cttcccaaag tgactcaata gtaccaacat ctttccaata accactaaat
     7681 tcgtaagcat aaacactttc acctgactca aggtaatttg gaatgacatt tttaccaaag
     7741 tctgacatgc caaccttgct cttttcagca gcgactaaca tattacgaag gcgttgccaa
     7801 tcaaaaatgt agattcccat agaagctttt gtagatttag gttgagctgg tttttcttca
     7861 aattcaacaa tacgattgtt agcatctgtg ttcatgatac caaaacggct tgcttcttta
     7921 agagggacgt ctaaaactgc tactgtcaag ctggcattat tatccttatg agactggagc
     7981 atatcatcat agtccatttt gtagatgtga tccccagaca aaatcaagac atactcagga
     8041 ttgacactgt cgatatagtc gatattttgg taaatagcgt gactagtccc ctcaaaccaa
     8101 cgatttcctt cacttgcaga ataaggttga agaatagaga cacctgaatt aataccgtct
     8161 agtccccagc ttgaaccatt cccaatatgg ttgttgagag caagtggttg atactgtgta
     8221 acgaccccaa cattgtgaat ccctgagttg gcacagtttg atagggcaaa gtcaatgata
     8281 cggtagcgcc caccaaattg cacagctggt tttgcgatgc tttgagtgag tttaccgaga
     8341 cgagttcctt gcccaccagc aagaatcaaa gctaacattt catttttcat tttctactcc
     8401 tttttggttt ttatttgtga cggttttagt agatttcaag cgacgtttga ttttccatac
     8461 acttgctccc atagccggta gggtaaaggt taaggtctgc tcataatctt tccatagtcc
     8521 ttcttgcgtt tgaacagttt gattatgttc tttccaaacg cctccccact cttccaactc
     8581 agtattccat acttcttcgt aaattcctgc aacgggtagt ccgattgtaa aatctttccg
     8641 ctcaacaggt accatattaa agatacagac taacatttct ccctttttac ccttacgaat
     8701 aaaggaaaga acactctggt ctcgattatc cgcatcaatg atttcaatac catcatagct
     8761 ggtatcaatt tcccacagac agcgatgatc tttgtaaaac tggtttagct gagaagtgaa
     8821 atacttcatc ttagcattca ttgggtcttc taggttagac cattccaact gttcttcaga
     8881 tttccattct aggaattgac cgtattcgct acccatgaag agcaatttct taccagggtg
     8941 acaaatttgg tacgtataga gattgcgcaa gcctgcgaat tgattgtaac gatctcccca
     9001 catcttatgc atcatactct tcttgccatg aaccacttca tcgtgcgaga atggcaagag
     9061 ataattctcc ttgaaaacat acataaagct gaaagtcacc aggttaaagt catatttacg
     9121 ataaatcgga tcttcttcgt agaaacggag gatatcattc atccagccca tgttccattt
     9181 gtagtcaaat cctagaccac caatctcttt cattcccgta atcttggttg cagacgaact
     9241 ttcttctgca atcatcatca catctggata ttctaactta ataacttcat tcaagcgttg
     9301 aaggaaataa taaccttcat agttgagatt tccgccatct ttattaggtg tccatggagc
     9361 atcatcatag tccaaataga gcatgttgct aacagcatcc acacgaatac catccaaatg
     9421 atagacatca atccaatgct taatgcaaga aattaagaag gactggactt cattttttcc
     9481 aaggtcaaaa ttaagggcac cccaaccatg gttatgagcc ttattatggt cttggtattc
     9541 aaaagtcggt gtcccatcat aataagctaa ggcatcatcg ttgatggtaa agtgacctgg
     9601 tacccagtcc acaataaccc caatattatg ggtatgacac tcctcgacaa aatcttgaaa
     9661 ctcctctggt cggccataag catgctctaa agcgaagtaa cccataagct gataccccca
     9721 actcaagccc aaaggatggg acatcaaggg cataaactca atatgagtat agttcatttc
     9781 aacgagataa ggaatgagtt catccttgag ctgggcaaaa ctataaggac tgccatcaga
     9841 atttcttttc catgatccag cgtgaacttc ataaatattg acaggacgct cttcaaagcc
     9901 ccaacgtttt cttcgtgcca gccaaagtcc atccttccat ttcttctcag gaagctctgt
     9961 taagattgcc cctgttcctg gacgagcctc atatctgaca gcaaaagggt caatcttcat
    10021 cagttgatga ccattttgac gtgtgacatg atatttgtaa atatgccctt cttgagccat
    10081 attggtaaag acttcccaga ccccaaaatc atttcttacc attggaatct gattttcaat
    10141 ccagttggta aaatcaccaa ccaagtgaac agcctgagca ttaggtgccc aaacacggaa
    10201 ggtatagcca tgctctccat ttagttcttc cctatgtgct cctagataat gttggagata
    10261 aaaattttca cccgtcataa aggtttttaa tgcttctcta ttatccatat actccccttc
    10321 tcctgtaagc gttttctatg tttttattat actacctttt tagagaagat tcaagtaaat
    10381 tactatactt ctttaattat tttgaaaatc tacaacaagt tcacttactc gttcaattgt
    10441 aaatcaatat tttttcaaaa aattgcgaaa acgcctttct ttttatacta tagtgaaatg
    10501 aaataaaaca tgc
//
LOCUS       DAVKZB010000063        10321 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKZB010000063 DAVKZB010000000
VERSION     DAVKZB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.063"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(248..640)
                     /gene="rpsI"
                     /locus_tag="VXA80_001386"
     CDS             complement(248..640)
                     /gene="rpsI"
                     /locus_tag="VXA80_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV0353873.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRTG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(660..1106)
                     /gene="rplM"
                     /locus_tag="VXA80_001387"
     CDS             complement(660..1106)
                     /gene="rplM"
                     /locus_tag="VXA80_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV0353874.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1463..1780
                     /locus_tag="VXA80_001388"
     CDS             1463..1780
                     /locus_tag="VXA80_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV0353875.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1930..2742)
                     /gene="folB"
                     /locus_tag="VXA80_001389"
     CDS             complement(1930..2742)
                     /gene="folB"
                     /locus_tag="VXA80_001389"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEV0353876.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNRGIHILKESSVLAT
                     EPWGGVEQDSFTNQVVEVETWLPAQVLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2785..3339)
                     /gene="folE"
                     /locus_tag="VXA80_001390"
     CDS             complement(2785..3339)
                     /gene="folE"
                     /locus_tag="VXA80_001390"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV0353877.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHLLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3320..4642)
                     /locus_tag="VXA80_001391"
     CDS             complement(3320..4642)
                     /locus_tag="VXA80_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0353878.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4644..5618)
                     /gene="folP"
                     /locus_tag="VXA80_001392"
     CDS             complement(4644..5618)
                     /gene="folP"
                     /locus_tag="VXA80_001392"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV0353879.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5713..6420)
                     /locus_tag="VXA80_001393"
     CDS             complement(5713..6420)
                     /locus_tag="VXA80_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353880.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6473..7891)
                     /locus_tag="VXA80_001394"
     CDS             complement(6473..7891)
                     /locus_tag="VXA80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV0353881.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8081..8893
                     /locus_tag="VXA80_001395"
     CDS             8081..8893
                     /locus_tag="VXA80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0353882.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9232..10251
                     /gene="adhP"
                     /locus_tag="VXA80_001396"
     CDS             9232..10251
                     /gene="adhP"
                     /locus_tag="VXA80_001396"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV0353883.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3045 a         2329 c         2011 g         2936 t
ORIGIN      
        1 actatcctcg gtttgtccta ttcttatttc acattactat aaaaataagg taaacccttg
       61 agtgctttga aacgttgata taatcgtatt gattaacgtt ttgaggcaac tgaccatgtc
      121 aggttgcagc cacatttgta agcgactaaa gtcgcaacaa ctgtgtcaat tgcaccaatt
      181 tagtaagaaa gtataaaaaa agaacacccc gaaaggtgct ctgtataagt atagtaattc
      241 tttcgaatta acgtttacta aattgtgatg ctttacgagc tttcttaaga cctggtttct
      301 tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgt gcgtttcaat gaatcgcgga
      361 agtctgggtc tacttgaaga agggcacgag cgataccgtg acggatagct cctgattgac
      421 cagcgtatcc accacctata acgttaacga aaacgtcgta tgaacctaca gttgaagtaa
      481 ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg tgggatgtac tcttcaacat
      541 cttttttgtt aacagtgatt ttaccagttc ctggaacaag gcgaacgcgt gcaacagcgt
      601 ttttacgacg tccagtacct gcatattgtg cttgtgacat actttatttg ttcctttcct
      661 tagataagtc ctgaaatgtc aagaacttct ggttgttgtg cagcatgagt gtgctcagct
      721 ccaacaaata ctttcaactt cataccttga gcgcgtccaa gtgtattgtg tggaagcata
      781 cctttaactg atttctcaat caaacgtact gcatttttag aacgaagttc acctgcagag
      841 atttgtttca atccacctgg gtggtttgag tgagtgtagt agattttatc agttgctttt
      901 ttaccagtca atttaacttt ttcagcattg ataacaatca cgaagtcacc tgtatcagtg
      961 tgtggtgtaa atgttggttt gttttttccg cgaagtacgc tagcaactac tgcagaaaga
     1021 cgtccaagtg gtacatcagt tgcgtcaact acgtaccatt tacgttcaac ttggcctggt
     1081 ttagccataa atgttgtttt gttcatgatt tctcctatat atagttcgat ttttgtttac
     1141 ggtgatgggt gttccttccc atcgaaaagt atttggaagg ttccggggcc tttcaaatgg
     1201 ggtaaacaat accgcctact atcataccaa atttcacccc taaaagtcaa tagatatgaa
     1261 aaatattttt cggtaacttc tcaaagtaac tgaaagttat ataatcaagt taatttggga
     1321 atttacctat taacttcggt agatttttgg gaattttttc ccttcttttt ccttgtagtt
     1381 aaaatccttt tcaaaaaatc accttttctt gatttttaat cctatttgct gtatgataag
     1441 gaaaaagaaa ggagacagag atatggcttt taccaatacc cacatgcggt ctgctagttt
     1501 tggcattgtt accagcttgc ctgatgacat cattgactct ttttggtata ttatcgacca
     1561 tttcttaaaa aatgtctttg aattggaaga agaactcgag tttcaattgc ttaataacca
     1621 aggaaagatt accttccact tttcaagtca acacctccct acagccattg attttgactt
     1681 taaccatcct ttcgaccctc gttatccccc aagagtactg gttttagaca tggacggtag
     1741 agaaactatc ctcctcccag aagaaaatga cctattttaa tactcaatga aaatcaaaga
     1801 gcaaactagg aagctagccg caagctgtac ttgagtacgg taaggcgacg ctgacgtggt
     1861 ttgaagagat tttcgaagag tataaaaact ctagccttca gttgcaagtg actgaaaact
     1921 agagtttttc tattttttca aagcatcata caagttgcgg atcggttgtt ttaatatcgg
     1981 atggataaaa tgaggcgcaa tttcctgtaa ggactcaagg acaaaaaggc gttccgctat
     2041 gtaaggatga ggcaatatga ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa
     2101 gtccaaatca atcaaacgag ggccccaatg cacttctctc acccgtccca gctctgactc
     2161 aatggctaac aaggtttcta atagcacttg ggcaggcaac catgtttcta cttcaaccac
     2221 ttgattggta aagctatcct gctcaacacc accccaaggc tccgtcgcta agacactgga
     2281 ctctttgaga atatggatac ctcgatttcg cattttctca atggcttgtt tcaagtttgc
     2341 ttgtttatct cccatattgc ttcctagggc gataaaggct cgttgcttgc ggcgatggat
     2401 ggttaccgag caagtatcta gtgacaaatg caccggtgcc caaggttttt tcagttccaa
     2461 cttcatttct tggacaagag gataagactc aaaggtacgt tccaccagtt tataggctac
     2521 cgtttcaatc aaatcttcac tcgtttcctg aaaccaagtc gtccactgct gacacaattc
     2581 tccgtaatgg acagaggctg ttaaatccaa gtctgtagct gccttggtca tatcatagga
     2641 taggatggct gaaacgacaa atttctgccc caattctttc tcactaggaa aaagaccatg
     2701 ataggcaaac atttccaaat ccttaatctg cagttgatcc ataatgattc ctttctagaa
     2761 aaatccgctt gaaacggatt ctttttatag ccccattaaa cgataagctt ggtcgcggag
     2821 gtccttatct gtttcaaata gaccacgagc tactgtcgtc aaggttgcag tgcctggttt
     2881 tctaacacca cgcatactca tacacatatg ttccgcctca atgacaacaa aggctccttt
     2941 agcacctaga tagtccatca aggcatcggc cacttcgata ttcaaacgtt cttgaatttg
     3001 tggttttttc gaataaactt caaccgtacg ggctagctta gacaagcctg ccacacgacc
     3061 atctggaatg taggcaatgt gcgctctacc ataaaatggc aataagtggt gttcacacat
     3121 ggtatggaaa aagatatcct tttctaccac catattatcg tcaataattt caaaggattt
     3181 tgacaaatgt tcctctgctg tttgaccaag acctgaaaaa atctcttgat acatacgggc
     3241 tacacgagca ggtgtttcct gcaagccctc gcgattagcg tcctctccta cagcctcgat
     3301 aatcattttt acagctgctt caatcttttg tgtatccatt ctcgttcttc ctctccatca
     3361 gataggccct cacttggctc aagaaataca aggaacctgt gacaatccta actgtttgtt
     3421 tctcttcttt tttatctgtc aaattctgct ctagaaaatc atgccaatct tggtagctga
     3481 gatttctaga cttagctgcc tctttcagca cgctttcatc cgtcgcccga ctatccgcaa
     3541 aatgtgttag agtaagctcg gtatctggca tggctcccag caagtccaac atatcctcca
     3601 aggccttggt tttgatacaa gtgaagagga tttccttatg ataatccgca aaacgttctt
     3661 gcaaggttac caacaaggcc ttgatagcat ggggattgtg ggctccatcc aaaatcatca
     3721 agggatctct tgacacgatt tccaaacgcc ctggccaact tgtttcttcc aaggcttgac
     3781 caagaaagtc attgcttgct agctctcgac catcttcttg acaaaaagta tcaagtaaag
     3841 ctatggccat cccagcattc tctatttggt acaaaccaag caggctagtc tggaagcgac
     3901 cttgtctgac agcacttgta tagtcaaaga cttcccctgt caccacactt tcttgatgac
     3961 gaacctgata atctgtcccg taggcaagtc tcggcgcatc tttcccttcc gcaatgcggt
     4021 caatcacagt caaggcttct ggagcaatac gccctgttac caagggcatc ccttgtttga
     4081 taatacctgc cttctgctct gctatggcct ccaaggtgtc accaagtagg gccacatgat
     4141 ccaagccaat agttgtaatt cctgtcaaaa tgggctgaca gacattggta ctatccaaaa
     4201 gtccacccat gccaacttcc atgatagcca catctacttg ctctgaggca aagtagtcat
     4261 aggctagggc tgtgataatc tcaaactcgg ttgtgccctg taaattggcg accgcttctc
     4321 cctccagcaa agactgatag tctgccatga gagcttctag cctcgcttct gagatcgatt
     4381 ccccattgat gctaatctgg tctgtgtaat gaatgagata gggcgaacta aacacgccaa
     4441 ctctcagccc tagcttttct agcatctttt tcaaaaaagc aatagtcgag cccttgccgt
     4501 tagtccctcc gatatggaga accttgagtt tgagatgggg attgccacgc aaagctaaca
     4561 gttccaccat tcgttccaag ccaaaatgcg gttgatccgt ccggtagtta gcaatccact
     4621 gattgttttc aatttctttc atcttattta tattgtttta aatctaaatt ttccgcttca
     4681 tcagccagac gaatggcaga ggcaatttca actgccatcc tgtgactagc tacgtcatgc
     4741 acgcgcacca cttctacacc ctgtcttgca gcgatactgg ttacatgagc cgaagccgtg
     4801 tcccgattgc ggaaaccaag ctctgtctca ggattgactt caaaaccatt ctcctctagg
     4861 atattgatga caaatcgctt gcgcgacact ccgagaaaga ttggatagcc cttctgatgt
     4921 agtttatcca ggtcccgtaa aagaagcaga ttttctttct tggtcagacc aaagccaatt
     4981 cctggatcca acaggatatt ttctggtgca ataccagctt ctgccgctct cgctagtgct
     5041 cgttcaaaga aagcctccat caattcttcg attggcaatg tttcaaagtc cgctaactct
     5101 tcctctgtaa aagcttgacc aaaaccaaaa tgagggaaga tgagcgagct agggtgctga
     5161 ggtcgcgcca taactggatt aaacatgatg accacttgcg ctctcgcttc agctaccaca
     5221 tgagccattt tctcatcacc cataagacca gtgatatcat tgactagatc ggcaccagca
     5281 gccaaagcag cctctgctac ttgactcttc caagtatcaa tagagatgag gacatcactt
     5341 tccttgcgaa tcgctttgat cactggaaca acacgctgga tttcctcttc tatctcaaca
     5401 tagctacttc ccggccgagt cgattctccg ccgatatcga gcatactggc tccttctgct
     5461 atcaatttac gagcctgctg gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa
     5521 aaggagtctg gggttacatt gataattccg caaataactg tctttgcatg attggcttta
     5581 cttgacatat cggtcactcc ctcaaggatt ttcatcatat tatttttcta ttttaccata
     5641 aaaagaaaaa gatggacacg attgcattca tctttcccta gtagaaagag gtcggttcct
     5701 acataaaaac ttttacacag aaattcctaa tgtccgactc ataatcacca caagagccaa
     5761 caaacagaaa gcaatcccat taacaatcat gtgaagcaag atcgacattt ccaaacgttg
     5821 ggtcttgtag gccgtccaag atagaactgt cgacatacct ccataaatca ataaagaagg
     5881 taaattactt ggttgatgca ataaagcaaa cacaatcgta ccgactacaa atcccaagtt
     5941 ctccttgcct cggaaaatct ttttaggaac aagcccacgg cacaagattt cctcacaaat
     6001 tggagcaagc aaggctagca agaagaagct agaaatcaga gaactattct gaaccatatc
     6061 gttaatctga gactggttag ctgttgtcgt ctcatttgac agttgcaaca agatagaacc
     6121 aagtatattt gaacagataa tgactaaata actcaagccc aaacgtgcca aatccttagt
     6181 tctaaaaaaa gaaaaattaa aacttgccaa ttgcgtttta cgagctccaa taataaataa
     6241 caccagaacc acaattgaaa ggccagcaac tatcagccct gactgtagca gggctacttc
     6301 ttttaaagtc aaaatagagg taaccactag gggaacctga tataaaagga cggcaagtaa
     6361 aaagaccaaa atccagcctg cacgattcaa caattctttc cacatatttt tctctttcat
     6421 cacctatctc ctttgtatta aaaaaggggg gaaatccccc tgggtcattc tattataagg
     6481 ccatgctgat gtagttaagg ataaacaagg catccaaaat ccaaatcatg gcatgaacat
     6541 ctttaacttg acctttgaca agcttagtca aagtgtaagt caagaaacca actgcaatcc
     6601 cttgagtgat agagtagctg aatcccataa agatagatgt gaagaaggca ggaactgctt
     6661 cagacatatc atcccaatgg atatttttca agctaccaag catcataatc ccaacgataa
     6721 tcaagattgg agctgtagcc gctgttggta cgatcgctag aagtgggcta aagaagcttg
     6781 aaatcgcaaa acagatagct acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga
     6841 taccagcagc agactcaaca taagtcgtta cgtttgaagt acctgcaatg gcaccgactg
     6901 tcgttccaat caaatcagag taaagagcct tatccaattt ggctgattgg tgattttcac
     6961 catttgtcgc tacaatacca actttttcac ctgtaccgat caaggtacca attgtgtcaa
     7021 aaatatctgt caatgagaag gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat
     7081 ctgaaaccaa agctcccaat ccttctgaac caagagctgc accaaagatt gtcttcaaat
     7141 cttcaaaagc tgcaccaaca tggttattag caaaatcgat actagacaaa tcaaccaaac
     7201 caactgcaat agcaagaaca gttgttgtca agatagagag aataattccc cctttaatcc
     7261 ctttgatgac aaagaagata gtaatggcaa gtcctgcaag agccactaaa acagctggat
     7321 tattaaagct gaccaatcct ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt
     7381 tgtcagcccc ttctcctaca acagtatagt tgcctggatc aatcgtaaat ttcaaaagtc
     7441 cagcattctt aatccctaca taggcaagga agacaccgat accagctgaa atagccgagc
     7501 gaagggcatt tggaatcgat tcaatgatca ttttacgaac atttgtcaag gtaataacca
     7561 atgaaataat cccacagatg aagaccatag ctagggcttc ttgccatgaa taaccaagtc
     7621 caaatacgac tgtaaaggta aagaaggcat tgagtcccat acctggtgct tgagcgtatg
     7681 gcaagttagc atagaaggcc atcatcaagg taccagcaac tgcaccgata atcgttgcaa
     7741 gaaacacacc ctgagcaggc attcctgttt gcgaaagaat ttgtgggttt acaaagagaa
     7801 tatagctcat tgcaaagaaa gttgttaaac cagcgagaac ctctgtacga acgtctgtac
     7861 cgttctcttt tagtttaaat aatttgtcca tcatcaatct ccttttaatt tttacgaaca
     7921 ataatagaat tatatcattt atcattcact ttttcaatgt ctttgtttga tttgtttaag
     7981 aaattaatcc aatttcattt tttgtttgga atctgctttt ctgcctgctt tctgttataa
     8041 tagaagacat cttatctgaa aacacactag aaaggtccat atgacgaaaa aaattattgc
     8101 agttgaccta gatggaaccc tgctcaactc agacagtcaa atttctgact ttaccaaaaa
     8161 aaccatcaaa aaagttgctg aaaaaggcca tcaggttatt attacgacag gtcgccctta
     8221 ccgtatgtca aaagattttt accgtgaact gggcttagac actcctatga ttaacttcaa
     8281 cggatccctt actcatttac cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga
     8341 caaaaaatat ctgctagata tggttcaacg ttcagaggac attcaagccg attttatcgc
     8401 tggagaatat cgtaaaaaat tctacattac aaatcccaat gaagaaattg ccaatcccaa
     8461 actatttggt gtagaagctt tccagcctga agatcaattc cagcctgaat tggtgaccaa
     8521 ggaccctaac tgtatcctct tgcagaccag agccagtgac aaatatgcct tggcaaaaga
     8581 aatgaacgcc ttctaccagc atcaactttc tatcaatacc tggggaggtc cgctcaatat
     8641 ccttgaatgt accccaaaag gtgtcaacaa ggcctttgct ttggactact tgctcaagat
     8701 aatgaatcgt gacaaaaaag atttgattgc ctttggagat gaacacaatg ataccgaaat
     8761 gctcgctttt gctgggaagg gttatgccat gaaaaatgcc aatccagagc tactccctta
     8821 tgcagatgag caaatttccc ttaccaacga ccaagatggg gttgccaaaa ccctacaaga
     8881 cttattctta taacctatac tgatactcaa tgagaatcaa agagcaaact aggaagctag
     8941 ccgtaggttg ctcaaagcac tgctttgagg ttgtagatga agctgacgtg gtttgaagag
     9001 attttcgaag agtatgacca gcttgcaagc tggtctttgt gctcttttca cagtctcatc
     9061 aaccagttaa tcgattgttc tactttttgt ctccaaaacc ttggaaaagg ctttcttttg
     9121 tgatatactt cacatataaa tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa
     9181 tcaatccagt cccgttgtcc ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct
     9241 gttgttgtaa atccagaaag cactggtgtt gctattgaag aaaaagtact ccgtccactt
     9301 gaaactgggg aagcacttgt agaagttgaa tactgtggcg tttgccacac cgacctccac
     9361 gttgctcatg gtgactttgg tcaagtccca ggacgtgttc ttgggcacga aggtatcggt
     9421 atcgttaaag agattgctcc agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt
     9481 gcttggttct ttgaaggatg tggcacttgc gaatactgta caactggtcg cgaaaccctt
     9541 tgccgtacag tgaaaaatgc tggctactca gtagacggtg gtatggctga acaatgtatc
     9601 gtaactgctg actatgctgt caaagttcct gacggacttg atccagccca agcttcttct
     9661 atcacatgtg ctggagtaac aacctataaa gctatcaaag aagcaaaagt tgaaccaggc
     9721 caatgggttg ttctttacgg tgctggtgga cttggtaacc tcgctgttca atacgctaaa
     9781 aaagtattca atgctcatgt tatcgcagtc gatatcaaca atgacaaact tgcccttgca
     9841 aaagaagtag gcgctgacat tgtgattaac ggcctcgaag ttgaagatgt agctggactc
     9901 attaaagaaa aaactgatgg aggagctcat tcagctgtcg taactgctgt gtctaaagtt
     9961 gccttcaacc aggctgttga ctccattcgt gctggtggtc gcgtcgtcgc tgttggtctt
    10021 ccttctgaaa tgatggaact cagcatcgtt aaaacagtcc tcgatggaat ccaagtcatc
    10081 ggttctcttg tcggaactcg taaagactta gaagaagcct tccaatttgg tgcagaaggt
    10141 ctggtagtcc cagttgttca aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa
    10201 atggaaaaag gccaaatcca aggacgtatg gtactcgact tcacccacta atctaataga
    10261 aacctatttt aaaagttgga agcttcttcc aactttttta tattaaattt taaataagaa
    10321 t
//
LOCUS       DAVKZB010000064        10321 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKZB010000064 DAVKZB010000000
VERSION     DAVKZB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.064"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            108..956
                     /locus_tag="VXA80_001397"
                     /pseudo
     CDS             108..956
                     /locus_tag="VXA80_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1213..7929)
                     /locus_tag="VXA80_001398"
     CDS             complement(1213..7929)
                     /locus_tag="VXA80_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV0353884.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESIAG
                     ESVVYADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKK
                     EDEAVTPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFN
                     QNWYFKLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAW
                     YRKTFKLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDG
                     RENVIAVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKV
                     ETHVTSKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVE
                     RPKLWTVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFH
                     GVSLHHDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQ
                     EEAFDTWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSI
                     GNEIGEANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDA
                     VGFNYSEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSD
                     YGNDRVGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGI
                     VDTAGIPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSN
                     ASSVELFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDE
                     SGKEIARDKITTAGKPVAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVR
                     FQLHGQGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAH
                     SDLLKSNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERP
                     VTWSSVDVSKPGIVTVKGMADGREVEAHVKVLAIAKELPTVKRIAPNTDLNSVDKSVS
                     YVLTDGSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAP
                     VVPTVTVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRAS
                     IFIQPKDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQA
                     VLPADKVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIA
                     QSIRPVNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKV
                     EGIDLEARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDVIR
                     PEQYAKEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNP
                     SDPVSNVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHE
                     DHGVGVPKSYVIEYYVGKTVPTAPKNPNFVGNEDHVFNDSANWKPVTNLKAPAQLKAG
                     EMNHFSFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLA
                     NFNPDLTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEY
                     RLHFTKDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEE
                     VPAENLTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATN
                     DIDKNSHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAK
                     LHFFADSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADK
                     DIAAGDEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVD
                     GKELADFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQ
                     VKEYRIHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQE
                     GKTGKERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKP
                     IDSSEASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8179..8256)
                     /locus_tag="VXA80_001399"
                     /pseudo
     CDS             complement(8179..8256)
                     /locus_tag="VXA80_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(8279..9754)
                     /locus_tag="VXA80_001400"
     CDS             complement(8279..9754)
                     /locus_tag="VXA80_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV0353885.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            complement(9802..10107)
                     /locus_tag="VXA80_001401"
     CDS             complement(9802..10107)
                     /locus_tag="VXA80_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0353886.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            complement(10145..>10321)
                     /locus_tag="VXA80_001402"
     CDS             complement(10145..>10321)
                     /locus_tag="VXA80_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0353887.1"
                     /translation="DKEVEVCLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKIS
                     EPEALYAYIAEAIA"
BASE COUNT         2830 a         2244 c         1993 g         3254 t
ORIGIN      
        1 ctcttatctc caaaaaaagc aacggatttg ccgttgcttt ttttgcatgg ttacgatagt
       61 cttggtaaaa tagaattgcc caataaacca tttagaaagg ctattccatg catattcact
      121 ataacacaaa tcaaacaact ttaccactaa aaatcagttc cttcttacca caagaccatc
      181 tcgtctttac tattgaaaaa gtggtgaata ccttggagga acgtcacttc tacgccttct
      241 atcatgcctt tggtcgcccg tcttatcacc ctaaaatgct tgtatctact cttctatttg
      301 cctattcaca agggattttc tctggtcgaa aaattgaaaa atggaagagt tagtgacctt
      361 agattgtttg tttattaacg gaactaagat tgaagccaat gccaacaagt atagttttgt
      421 gtggaagaaa acgacagaga aattctccgc caaacttcaa gaacagatac aagtctattt
      481 tcaagaagaa atcactcccc tcctgattaa atatgccatg tttgataagg aacaaaagag
      541 agggtataaa cagtcagcta aaaacttagc gaattggcac tataatgaca aggaggatag
      601 ctacacacat cctgatggct ggtattatcg ttttcaccat accaaacatc agaaaacaca
      661 gacagacttt caacaggaaa tcaaggttta ctacgccgac gaacctgaat cagcccctca
      721 aaaggggact atatatgaac gaacgctatc aaaacttgaa agctaaagaa tgtcaggcgc
      781 ttttatctcc ccaaggtaga cagattttcg ctcaacgcaa gattgatgtg gaacctgtct
      841 ttgggcagat aaaggcttgt ttgggttaca agagatgtaa tctgaggggc aagcgtcaag
      901 tgagaattga catgggattg gtacttatgg ccaataacct cctaaaatat agttaaatga
      961 aataagaaca ggacaaatcg ataaggacag tcaaatcgat ttctaacaat gttttagaag
     1021 tagaagtgta ctattctagt ttcaatctac tatacaataa gagaacgact caaaattaaa
     1081 aagctagagt tccacaattg gaaatatcta gcttttttgt ggttgagaac tattttgttc
     1141 cagactcttt atcttctatt taggacaaga gtttttcttt ggtctttaat gataaagaag
     1201 gtatcaaaat ttctagtctt cttttttacc tttagtaact actaatcctg cactcaaacc
     1261 tagaagagct aaacctgctg ctactgctgc ttggcttgcc gcactacctg tatttggtaa
     1321 ctgggcttta ttagtttgac tagcttcact tgaatcaatt ggttttgtat ctgctttttc
     1381 tgacacttgt ggttttttag cttcttgagc tactggtttg gttccaacca agacgatgcg
     1441 gtctgtcgga gcttctacca cttcacggag tttttcttcc ttacttccat caggattaat
     1501 cgctgtaaag atacgttctt taccagtttt tccttcttgt tctacacgag tttcacctag
     1561 atacagtgtt gaatcttttt tctcaactgt cttgtaggcc aaatcttttt caacaaattc
     1621 gagttttgga agatcttctt gtacagcagc aactgtcttc tcagaaactg gtttttcctt
     1681 agtcaagtgg atacggtatt ccttgacttg ttttccactt tctgaaacga ggcgaacaag
     1741 tactggaagg ctatcttctc cactatctac cacagttgaa gctacttgat tgttttcttc
     1801 aactgagact tttggccgtt gacctttata ggtaatttga tagtcttgac gattttcagc
     1861 gaaatcagca agttcttttc catctacaag aatcttcgat tgcgtgcttt cttgaggcaa
     1921 ttcacttggt gcaaggaagg tcatctcaat catcgcaaca ccgctcttat ctgctttacg
     1981 ctccatacgc catctcatag ctttggcttt gacagcttta aatgttacgt tgatttcatc
     2041 accagctgcg atgtctttat ccgcacgata aggcacagct tcccaatttt ctggattgtt
     2101 gaatggatgg tctgcgtcgt aggcttggta gtttgaatag taggttggca cttcaaactc
     2161 tggaccgaca tagcgttcta aaacgagttt agttggtgca tccgtaccac tatctgcaaa
     2221 gaagtgaagt ttggcttggg caacagtccg ttctacaatc ttaccatttt cacggaagat
     2281 cacacccgct gatacttctg gattagaaga tggtgttgga gaccagtttg tccaacgacg
     2341 attttctgaa tgatctccgt cattgagata gtcaacgcgg tcatgagagt ttttgtcaat
     2401 atcattggtt gctgaagcaa aggcctggtt actgttttca tcatagttag ggttatctga
     2461 aagagcttcg cctagtttgt ctgtcactcg tacagtgacc tcagcaacaa gatcactacc
     2521 aaggacatgg cctcgaacgg taaattgacc tgcttttgtc agattttctg ctggaacttc
     2581 ttcccattca actgtcaggt cttttgtttc gtagccgtct ttacctgtga agtaaactgg
     2641 aactttagtc ggcaattcaa gtgcttgacc tacttgtagc aagcgagctt gtttaaccgc
     2701 agcaactggt ttatgagaaa gtaagctctt atccttagtg aagtgcagac ggtattctcc
     2761 taagatgtcg ccattttcag ctttcgcgat gacacgaact ggctcacctt cacgaacgct
     2821 tggaacgacg gtagcgagac cattgttgct aacacttgct gtgaccgccg taacttttcc
     2881 atctacagac tcaaggtagt agtctgtcaa atcagggttg aagtttgcta agtctttgcc
     2941 gtcaacttgg attcttgttt gtccttgttt ggctgccgca acttgtttcg caaagatttg
     3001 tacctctgtg atagacgttc cacgcttgtt atctgcttta accatgcgaa tacgaacagc
     3061 ataggtttca actttatcaa agctaaagtg gttcatttct ccagccttga gttgagcagg
     3121 ggcttttaga ttagtaactg gtttccagtt ggcagaatca ttaaagacat ggtcctcatt
     3181 accaacaaaa ttagggtttt taggagctgt tgggacagtc ttaccaacat aatactcaat
     3241 cacataagac ttcggtacac caactccatg gtcttcgtgg aatccgacac ttagattatc
     3301 aacggagcgt ttgctcaaga tacctgaatc tccaaacaga acaccgactg aagcttctgg
     3361 attggtacga ttccagtttg tccaacgatt ggctggttgg ttattgtagg aaatgagctt
     3421 gtcattaaca tttgaaactg ggtcgcttgg atttgagtct gaagcaaagg caagtggcaa
     3481 ttctgaaccg gtccattggt cagaaatgtt tgcaccttgc tcagtttgag cagatacgcg
     3541 aacatgaagt ttagttgtta attgagtacc ttctaagcga ccattaactg taaagacacc
     3601 ttccttagcg tattgctctg gacgaatcac atcccatgca accttagctg atgaaacgtg
     3661 accatttgaa tcatatgtcc gaacactttc tggtaattgt ggtgcttctg cgattggagt
     3721 tgtcacactg acttcttcaa ctgaaacgat accttctaca gagacttttg cacgcgcttc
     3781 aaggtcaatt ccttcaactt tacctagtac ttcaaatgtt tgataggagt ctagtttttc
     3841 tttcggaata gcttgccaag tgactttatg agttttaggg aaacctttgt catactcaac
     3901 tgttactgtt gctggaagac ttggttcctg atgcaaatct gtcactacat ttacaggacg
     3961 gatggattgc gcaatcttct tctcagtatt ggcttggata gtgagttcaa cttggccttt
     4021 agctccctca tattcagcgt tcagagtgac tgctcctggc ttatgcaact caagcattcc
     4081 tttacgaatt gcgacttccc cttcaccact tgtagagaag gttactttat cagctggtaa
     4141 tacagcttgc gttccatctt gatagtgagc tcgaaccgac aatttgacag tttggtcttc
     4201 tttgagactg tcagcttttt ccacttgcaa actcaagtga gcaatttttg gcgcttcttc
     4261 aaggaattga attgcatagg tttgaagagg gccaccatct ttaggctgaa taaagatgct
     4321 cgcacgcatg ccgtttgctg cgcttgcttg aagaactgtc acatcagcat tttcagcact
     4381 tgctgtgact tctggcaatt gggcaccata agatagagta cgatattgca ttggttgtcg
     4441 actagtaaga cctgttactg cttcacctcc aacagtaaca gttggcacta caggtgctgc
     4501 agcatttcct tcttctacca caagggttgc atgaatagtt tctccagcta actgaccagt
     4561 catttgaata cgtgatccag ctactgaaag tttggcttta tctacttccg taatctccca
     4621 gctatctact tcgtactctt gtacacttcc atcagtcaaa acataagaaa cagatttgtc
     4681 tacagaattc aagtcagtat ttggagcaat acgtttaact gttggtaatt ctttcgcaat
     4741 tgctagaacc ttgacatgag cttctacttc tcgtccgtca gccatacctt tcaccgttac
     4801 aataccaggc ttgctcacat ctactgaaga ccaggttaca ggacgttctg cacggctacc
     4861 atcactgtat acaaacggaa cagtggtagg catttcaggt gcctctccaa taatggtctg
     4921 tacttttggc acttctgtcc ccaaaacagt cttctcttgt ccttctttct taccagtaaa
     4981 gacagtgact tggttcgatt tcaagagatc agagtgggca gtaagggtga atttccctgc
     5041 ttgttcagtt gatttgacaa tggcaacacc tttaccatta aatgctttac gaatccaaga
     5101 accatctgct tgcgccttat agcgttcacg actggcttgt tctccgttat ctacaccgac
     5161 cagttgacct tggccatgca attggaagcg aaccagatta ttagcagttg gaaccacatt
     5221 cccctggctg tcaacaattt catagtagat gtaagtcaag tcttttccat ctgctgcaat
     5281 cgcatggtct tccttaataa gacgaactgc cactggctta ccagcagtcg taatcttatc
     5341 tcgagcaatt tccttgccag attcatcacg agcaattgct tccaaggtac ccggttgata
     5401 ggcaactttc cattcaagat aaagttcatt agcatttgca ccttcttggt aagtccgccc
     5461 atcgctggtt tgttttttat tgaaagtctt aagaccaaga gattttccat tcaagaacaa
     5521 ttctacacta gaagcattcg aataagcacg aactggaatc ttaccttctg agtcagctac
     5581 tttggatgct aattctttgt tttcccagtt ccagtgagga agaaggtgta ccatcggttt
     5641 ctttttaaca gaaacccatt ggctttggta gaggtagaag tcatgttttg gaatgccggc
     5701 tgtatctacg ataccaaagt aagagctctt aacaggagtt tgattttggt tgtgccatgg
     5761 tgtaggttca ccaatatagt ccgtacctgt ccagataaac tgtccagcat agccagcgtt
     5821 gtcacggtca aaagtccatg aagcggttgc tgttttcccc caaccaacac gatcattccc
     5881 atagtcagac tgttcataat tacgctcagg tccattgcta tgtttcaatt cacgttcagg
     5941 gcgatagtaa cttccacgtg tacgggtagc tgaagatgtt tctgatccat aaatcaacca
     6001 ttttggatgc ttagctctaa gggctttgta attatcttca gaatagttaa atccaacagc
     6061 atcgagttca tcagcaattt tctcatgccc tccgctacca ttaccgaaac ggaatttatc
     6121 tgctcccatg gtaacatagc gagtcttatc aacatccttg ataaccttga ccaaacgttt
     6181 aacagttgct aaagagtggg catcaccatt agcttcacct atttcattac caattgacca
     6241 catgaagata gcagggttgt ttttgcctct ttcgaccatg gtacgtaggt caaaatcaga
     6301 ccatttttca ccttttcgag cttctgggtg agtggcatct ttttcaaaaa aacgtccata
     6361 gtcataaggt ttcttgccac cataccaagt atcaaaagcc tcttcctgaa cgagtaaacc
     6421 tagttctgct gcgatttgca aggtttgctc actagcaggg ttgtgggttg tacggatgga
     6481 gttaactccc atctccttca tttgtttgag acggcgatat tctgctttat agttttcttc
     6541 tgctccaagc gccccatggt cgtggtgcaa ggatactcca tggaatttaa tacgttcacc
     6601 attcaaagag aaaccttcat ttggagtcca gtgatagtaa cggtaaccaa acaaatcctt
     6661 cttagcatca accaattgac cgtcacggta aacacgcgta atcaattcgt acaaggcagg
     6721 tttgtcattt aaaacggtcc agagttttgg tctttcaact tctaaaatcg catctaggct
     6781 tgttgattca tgtgctttta aggtacgact cgctgtacga actaagcctg ttacagcatg
     6841 accacctcgt tcaacgattt gatattcggc tacaagttca tggtctttgt cgtccgtatt
     6901 gacgattttg ctggtcacat gagtttcaac cttgccatgt tgttgttctt caagttttgg
     6961 tgttaaaata gttgtcccat ttttctcaac atgcacctta tctgtcactt gtaaagtcac
     7021 atcacgatag ataccacttc ctgaatacca acggctactt ggctgtttgt tgactgcatg
     7081 gacagcaatc acattctcac gaccatcttt ttgaaggtat ttggtgatat catatgagaa
     7141 ctggttataa ccatttggat aatgccccac taactgacca ttgacataaa cttgagaatc
     7201 catgtagacg ccatcaaaag taaggcgaac atttttcttg aggtcttttt catctagttt
     7261 gaaagtcttg cgataccaag cttccccacc gttgagctgt ccaccttcat tttgtgcagg
     7321 agattcatga tcgaaatcgt taaagatact ccagtcatac ggtaaatcta attttttcca
     7381 cgtagatacg tctgcatcag gtttaatggc ttccttagaa tttgcattga gtttaaagta
     7441 ccaattttga ttaaaatcca ctttcctgtc ttcaatcatt tgattcactt cttcatttgt
     7501 tacagcttta gcatcttcct tgagcggttt ttcttgactt gaagcttgtg attctatcct
     7561 tggagctttt tcttccggtt tagcagacac tttttcctct tttggagtta cggcttcatc
     7621 ttctttcttc tcagatgcaa tagcctcagt tgaactaggt tcactttgtt ctgtcctttc
     7681 aactacattt ttagtttcca aagctttatc agccttttct tctactatca ttttttcctc
     7741 tttaggtttc tcagcagtat gagtaataag tgtttcatcc gcataaacta cagattctcc
     7801 agctatagat tctccagcta tatttcctcc taatagaact gcacaagtcc caatcattac
     7861 tgagcaagct cccacagcaa acttacgaat gctataaact cttttccgat tccaatggcc
     7921 tttccccata aaaccctcct tatattatat ttagtgcagt tagctactac caaagcccaa
     7981 gtggtataca tggtatgaca acctagtttc aacaatttac actctgcgaa aatccaattc
     8041 aaacttcgtc agtgtcgcct tgtcgtagat atgattactg acttcgtcag tttcatctac
     8101 aacctcaaaa ccatgttttg agctgatttc gtcagtttca tctacaacct caaaaccatg
     8161 ttttgagctg acttcgtcag tcttatctac aacctcaaaa ctgtgttttg agcaacctgc
     8221 ggctagcttc ctagtttgct ctttgatttt cattgagttt atattttata ggagcgcatt
     8281 attttgcttt tgctgcgtac tcttcgttac gtttgatcat ttgttttctg taccaagcaa
     8341 agataccgat atagaataca aggaaggcta ctgcaccaag gattgctttg atatcaccag
     8401 ttgtagtgtt accaattgtc caaccaagaa gtttttcgat tggtccttca agagtagagt
     8461 gagtaatcaa ttgagtttgg ctcacacctt ctgggaaggc acctacacct ttagcaagtt
     8521 ctgttgcaaa tggtgcaata agggtacctg aaagaaggaa gagtggcaac aagagtgttc
     8581 cgaagataat catacggagc aatttaccac gagttacaac caagagagct ggagtaacac
     8641 ccatagcgat gatacctgca agtggcaaga taccatttcc aacttttgaa agaagcactg
     8701 cttcaatcaa catgattggt gcaagtacgt tggcacaagc ccagatttca gcacgaccag
     8761 cgatgaatgg ccagtcaaga ccgatattga atttacgtcc ttgaagacgt ttagtagcaa
     8821 cgtttgtaat accttgtgat agtggttcta cggctgcgat gaaccatgaa ccgataagtg
     8881 agaagagttc caaagataca ccggcagtca aaccaagaga caaccatcct ttgataacaa
     8941 gacgccattt atctgcatct gcaacacctg caattggatg tggagttccc ataataccga
     9001 taacgatacc aaggatgaaa ccgatgaaga atttagatcc ccagaaaccg attttcttgt
     9061 tcaatttagc agcatcaaag tcatatttat caaggcctgg gaagaatttt tcaaaaatct
     9121 tatccaaaac catgataact gggttcatca tgtagttcat gtgagttgat gtcattggtg
     9181 atgaacttgg ggcgttaaga aggtcatcaa atgtaggttt catcaagtca gagttgataa
     9241 ttttcaacac accgacaaga acgatagctg ctgtagcaat caagagtgaa accccttgac
     9301 tcacaccatt gttatcagca taccatttaa tcaagagacc tgtgatagac aagtgccaga
     9361 tatcaaagat atcgacatca agtgtatcgg ttttcttcat agcaagcatc actacgttga
     9421 caatcaacat gatgagcaag aagtaaagtg tccaagcaga accccaagtg attgttgcaa
     9481 gtggtgccca accaacgtcg gtaatactca attgtatacc agtgttttca acgaattttg
     9541 caagtgatgc tgagaaagca ccatttagca taccgatgat agcaccgata cctgtaagag
     9601 cgatggcaag tttgatacca ccttcaagcg ctttggagaa tttcactcca aaaagtaaag
     9661 ccaatactgt caaaatgatt aacatgatga caggtccacc catttctaag atgggattga
     9721 aaacctttcc gattaggtca aagattgcat ccataacagt tcctcccttt tttatgttat
     9781 atgaatgtta acaaattaga attagcttaa tccgtgttct ttaatagctg cttcaatatt
     9841 gtcaaatact ggagcgctca ttgctggtat acggaataag attggcccag cttcgataac
     9901 tgggatacct ggttcaaaac caagatctgt tgcagcgatt ggtgtaaaga tatcgtaacc
     9961 tttcataagg tcttcgttta catctttcac catgactgca tcacagtgaa catcataacc
    10021 acggtttgaa agttcttctt ctagagcact tttaatttgg tgacttgagt taacacctgc
    10081 accgcaggca gcaagaattt taatcatttg gatttcctcc gattttattt tttaatagac
    10141 aagattaagc gattgcttca gctatataag cataaagggc ttctggttca gaaatttttg
    10201 ataggtcttc aagatgacca tttcctgtga agaagtccat caactgagca agaatgttcg
    10261 tttgacttga acttgaatta ttgatgataa agaagagtaa gcatacttct acttccttat
    10321 c
//
LOCUS       DAVKZB010000065         9832 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKZB010000065 DAVKZB010000000
VERSION     DAVKZB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.065"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     regulatory      86..174
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            389..1057
                     /locus_tag="VXA80_001403"
     CDS             389..1057
                     /locus_tag="VXA80_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV0353888.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(1207..1293)
                     /locus_tag="VXA80_001404"
                     /pseudo
     CDS             complement(1207..1293)
                     /locus_tag="VXA80_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1384..2166
                     /gene="thiM"
                     /locus_tag="VXA80_001405"
     CDS             1384..2166
                     /gene="thiM"
                     /locus_tag="VXA80_001405"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0353889.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2168..2797
                     /locus_tag="VXA80_001406"
     CDS             2168..2797
                     /locus_tag="VXA80_001406"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0353890.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      3006..3094
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3355..3915
                     /locus_tag="VXA80_001407"
     CDS             3355..3915
                     /locus_tag="VXA80_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0353891.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3916..5301
                     /locus_tag="VXA80_001408"
     CDS             3916..5301
                     /locus_tag="VXA80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353892.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     VQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5303..5953
                     /locus_tag="VXA80_001409"
     CDS             5303..5953
                     /locus_tag="VXA80_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV0353893.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5964..6656
                     /gene="tenA"
                     /locus_tag="VXA80_001410"
     CDS             5964..6656
                     /gene="tenA"
                     /locus_tag="VXA80_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV0353894.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTLNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6661..7185
                     /gene="thiW"
                     /locus_tag="VXA80_001411"
     CDS             6661..7185
                     /gene="thiW"
                     /locus_tag="VXA80_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV0353895.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALVMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            7185..7988
                     /locus_tag="VXA80_001412"
     CDS             7185..7988
                     /locus_tag="VXA80_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0353896.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            7981..8613
                     /locus_tag="VXA80_001413"
     CDS             7981..8613
                     /locus_tag="VXA80_001413"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0353897.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8822..9628)
                     /gene="thiD"
                     /locus_tag="VXA80_001414"
     CDS             complement(8822..9628)
                     /gene="thiD"
                     /locus_tag="VXA80_001414"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0353898.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLDSVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCT
                     FAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9712..9810)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         2877 a         1773 c         2136 g         3046 t
ORIGIN      
        1 aatctactat atataaagat atttttcggt caaatatctt gctagggctt tcattttttg
       61 ctatactggt tcagtaatta aataatagag acatttgggg tgctttaggc tgagataata
      121 cccattgaac ctgatacagt taagactgac gaagggaaat gtgaacagtt ttttcgtatg
      181 gtgaaatgaa cggtctaatc ttgtaagtca actctaattc ttgattgtaa ttggagtttt
      241 ttgtttatat aaactggata ggcttgttta gttagctctc tgcaaatgct ctcgatgtgc
      301 tttaaaagga tcaagtgaaa ttacgatttt gcttgatatt tcttggtgtt ttattaatta
      361 ctaacaagta aagagaggag aagtaaatat ggaaacacaa gactatgcat ttcagccagg
      421 tttgactgtt ggagaattat taaaaagcag tcagaaggat tggcaggctg caatcaatca
      481 tcgttttgtt aaggaacttt ttgcggggac aattgagaat aaggtcttaa aggactatct
      541 gattcaagat tatcacttct ttgatgcctt cttatccatg ctgggtgcct gcgtagctca
      601 cgcagataaa cttgaatcca agcttcgttt tgccaagcaa ctaggctttc ttgaagcaga
      661 tgaagatggt tatttccaaa aggcttttaa agagttaaaa gtagcagaga atgactatct
      721 agaagtgact ttgcaccctg taacaaaagc ctttcaggat ctaatgtatt cggcggtggc
      781 ttcatcagac tatgcccatc ttttggtcat gctggtcatt gcagaaggtc tctatttaga
      841 ctggggttct aaagatttgg ctttacctga agtctatatt cattcggaat ggatcaatct
      901 ccacagaggt cctttctttg cagagtgggt tcaatttctg gttgacgaac tcaatcgtgt
      961 cgataaaaat cgagaagatt tgacagaact tcagcaacgc tggaatcaag cagtcgcttt
     1021 agaattagct ttttttgata ttggttacga tgtctagaga aggctcatga tatttacaaa
     1081 tttgatgagg taagactctt cgaaaatctc ttcaaaccac gtcagtttca ccttgccgta
     1141 ggtatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac
     1201 ttcgtcagtc ttatctacag gctcaaagca gtgttttgag caacctgcgg ttagcctcct
     1261 agtttgctct ttgattttca ttgagtataa atcattcaac gtaaatgaaa aaaatttctt
     1321 aacgaaagat agagataaat cgaaatcagt agatcgtata aagtgtaaag gaaggatttc
     1381 aatatgacaa gtttaaaatt attaaaagaa aaagcaccat tggtcatttg tataaccaat
     1441 gatgtagtaa aaaatttcac agcaaatgga ttagtagcac tgagcgtctc accagccatg
     1501 agtgagtttc cagcagattt agaggatttg ttaaagtatg ctggtggttt attaataaac
     1561 ataggaacat tgacagatga aaattggaaa ttataccaag ctgctctgaa aattgcagag
     1621 aaatataatg tcccagcagt tttagatcct gtagcctgtg gagcaggaga atatagaaaa
     1681 aaagtagcag atgatctaat caacaattat aaactagcag cgattagagg aaatgctggc
     1741 gagattgcct ctttagtagg aatagatgtg gcatctaaag gagtagatag tgcgggcgta
     1801 gataatattg acgaaattgc tctagcagca aatgagaagt tcaatattcc aatagtagta
     1861 acaggtgaag tggatgccat tgctgttaat ggagaagtgg taacgattca taatggtagt
     1921 gctatgatgc cgaaagtcat tgggacagga tgcttattag gggcagtggt agcaagcttt
     1981 atcggactag aaaaaggtca agaattgaaa tcattagaaa cagcaatgtt ggtttacaat
     2041 atcgctggag aaatggcaga aaaacgtcca aatggacatc ttcctgggac atttaaagtt
     2101 gaatttataa atgccttata cgagattaca gatgaagatg taaaggaatt caaaagagtg
     2161 aagtaagatg tttcataaag aattactaaa actatatttt atttgtggaa cgactacttg
     2221 ccaaggaaaa aatctatata cagtcgttga ggaagcctta aaaggtggta taaccttatt
     2281 tcaatttcga gaaaaaggtg agggtgcttt agaaggctta gaaaaacttg agctagctat
     2341 ccaaattaaa gaactttgta aaaaatacaa tgttccattt atcgttaatg acgatataga
     2401 tttagcaatg gaaattgacg ccgatggcgt acatgtaggt caagatgata ttggtgttga
     2461 tgaaattaga aaattgatgc cagataaaat aattggtctt tctataagaa acgaagaaga
     2521 atttcaacaa tcaaaagttg aatatgtaga ttatgtaggt gttgggcctg tatttggtac
     2581 ccagtcaaag gatgatgctg gtggtgctat aggttatgaa ggtcttgaat tgatgagaaa
     2641 actattgcca caaatgcctt tagttgcaat tggtgggata cagacgaaac atattaaaga
     2701 cattataaag accaatatgg atggcgtttc aatcatttcg gcaatatcgt atgcaaaaaa
     2761 tatagaaaaa actgttcggg aaatgagtga acaatagata tctatccttg gattctaagt
     2821 aagtaggatt ttcctttttt aaaaagctat gtccagattc agcttgaatg gctaattcat
     2881 caaacaggtc atttgttttt tgcaaaagaa agatctgctg tctttgtgag tgcaaggctg
     2941 tatttccagt tcaatttctt gaactagtat aaaatgtgtg ttataataat cacataatta
     3001 aataataaag acatttgggg tgctttaggc tgagatgata cccattgaac ctgatacagt
     3061 taagactggc gaagggaaat gtgaaatgtt tttttcgtat gtcgaaaaga actgtctaat
     3121 cttgtaagct gaactctaat tcgtatctgt aattagagtt ttttctttta cgaaactgga
     3181 tgaagatgat atagtagatt gaaataagat gcgaacaaat caattagaaa attcaaatta
     3241 atttctagaa atattttagt gttcttgtgt actgttctaa attcagtctg ctatacaaag
     3301 tatttaggtc ggtcttcttg agtgccccac ttttctttaa aaaaggagtt ttttatgttg
     3361 aaaaaatggc agttaaaaga tgttatcttg cttgctttct tgtctatctt ttttggtggg
     3421 gttttcgttg gttcaggata tgtgtataat attctcagtc tactcttaac acctcttggt
     3481 ttgcaggcct ttgccaatga aatcctcttc ggtctctggt gtatggctgc gcccattgct
     3541 gccatctttg ttccgagagt cggaagtgca acgattggag aagtgctagc tgcgcttgct
     3601 gaagtccttt atggtagcca atttggtcta ggagctcttt tgtctggctt agttcaaggt
     3661 ttgggaagtg aatttggttt tatcgtaact aagaatcgct atgaaagttg gctctctcta
     3721 actgctaata gtattgggat tacgcttgtt agctttgtct atgaatacat taagttaggt
     3781 tactacgcct tttcccttcc gtttgtcctt tccttacttg tggtacgttt tatttctgtt
     3841 tatttcttct gtaccatctt ggttcgtgcc attgtcaaac tctatcatca gtttgcaact
     3901 ggaggaaaag catagatggg tctggaacta cgagcgattc agtccccaat cttctctgag
     3961 ccgtttgatt ttacttttca tgcgcaagcc tttaccttgt tagttgggag tagtgggtct
     4021 ggaaaatcca gcctctttca aatgattgtc caagttagtt ctcttcccta tagcggtcaa
     4081 gtcctgatag atgggagcga ggtcagtcag ctttctatcg tcgaacgtgt ccagacggtt
     4141 ggtattctct tgcaaaatcc taatcatcaa tttaccatgg agagcttgtt tgaggagttg
     4201 gtttttacca tggaaaatat cggctatcac cttcaggaaa ttgattctaa aatagcagag
     4261 gttgtccagc aatgtcgttg cgaggcaatc ttacaccgtc ccattcatca cctatcaggt
     4321 ggggaaaagc aaaaagcagc gctggctgtc ctctttgcca tgaatcctag ggtctatctc
     4381 ttggatgagc ccttcgcttc tattgaccgc aagagcagaa tcgagatatt ggagattcta
     4441 aaagagttgg tctatgatgg gaagacagtt attttgtgcg accatgattt atctgactat
     4501 aaagcctata tcgaccatat ggtggagcta agagacggaa aactaaggga agtgtttcaa
     4561 atcccttcct atgagatgac acaggttgct tcaaaggaag ttgcttctag cccggaacta
     4621 ttccatatga accgtgtgac tggtgagctt ggtaatcgcc ccctcttttc aattgctgat
     4681 ttcacattct atcaagggat ttcctgtatc ctgggtgaca atggtgtcgg gaaatcaacc
     4741 ctctttcggt ctattcttca atttcaaaag tataagggga gcattacttg gaagggttcg
     4801 gtcctgaaaa agaaaaagag tttgtatcgt gatctgactg gtgttgttca ggaagctgag
     4861 aagcagttta tccgagtcag tctgcgagag gagcttcaat tagatggacc tgattctgaa
     4921 agaaatcagc ggatttttca agctttacga tattttgatt tggagcaggc agtcgataag
     4981 agtccctatc aattaagtgg tggtcagcaa aaaattcttc agctcttgac catcttgacc
     5041 agtaaggctt ccgtgatctt gctagatgaa ccttttgcag gtttggatga tagagcctgc
     5101 cattattttt gcaagtggat tgtggaggag aggaatcaag gaagaagttt tctgctcatt
     5161 agtcatcgtt tagacccttt gatttctgtg gttgattatt ggattgagat gactagtcag
     5221 ggtcttcggc atgtgaaaga agtgaccatt accaaaccac ttacatctca gagtagcaat
     5281 acccaagggg aggtgagata gtatggtcaa agtagcaacc cagacaccga ttatcagtct
     5341 cttcttgctg attttatcct tggaaacatc tttcattcct tcgattgctc tgaatctttc
     5401 ggtagtcgca ttttgtattc tctttatgct ctattaccgt cgatttaaaa tgttagcttg
     5461 gatgatcata cttgccattt taccatcttt tgccaactac tgggcagttc agttacacgg
     5521 agatgcttca caggcagtca tgcttggaac gagggccttt gtgacagttt gtatcggcct
     5581 tgtctttgtt tcctctattt cactaaaaga gcttctcttg tacttggctc aaaaggggct
     5641 atcacgctct tggtcctatg ccttgattgt ggtattcaat tcctttcctc tcattcagca
     5701 agaaatcaag tccctcaaag aagcttgcct attacgtggt caagaactgc atttttggtc
     5761 acccttgatt tacagtaagg ttctgatgac agtctttagg tggcgccatc tttacctgag
     5821 agctctgtct gctcatggat atgacgaaca tgcacagttg aagaatagct atcggacttt
     5881 ttatattcct aaaaaaacaa aattaatcta cctgcttttc tttttattgc ttcaaaccag
     5941 tctattttta taaaggagtt attatggaat ttacagatat tgcgatggaa ttatccaaga
     6001 aagcttggca ggcttccttt catcatccct ttattttaca attacaagag gggaatttag
     6061 aacctgccat tttccgctat tacctgattc aggatgccta ctatctgaag gctttctcag
     6121 aaatctatca tcttttggct gataagactt taaaccaaga gatgaaaaga ctcttgaaac
     6181 aaaatgctca gggtttagtg gagggtgagt tatttattcg ccaacaattt ttcaaggaat
     6241 tggaaatcag cgaccaggaa atggagcaac atccaatcgc tccaacctgt tatcattaca
     6301 tttctcatat ttataggcaa tttgcagaac cgaacttagc catcgctttt gcaagcttgc
     6361 ttccttgtcc ttggttatac catgatatag gcaaatcact taatcttaaa ccatcaccaa
     6421 atcctctcta tcaacaatgg attgaaactt atattacgga tgagttagag caacagatca
     6481 gagaggaggg agcactggtc aatcagctct atcgagaaag tgacgagaca gacaagcaaa
     6541 aaatgctaga tgccttccac atcagtgttc atatggaagc caagttttgg gaaatggcct
     6601 accaacacca gacatggaag agcgatttac agtctttaga aaaaggagaa gaatagaagc
     6661 atgagaaagc accaattaca agttcacaaa ttaaccattt tatctatgat gattgccctt
     6721 gatgtagtcc ttacacctat ctttcgaatt gagggaatgg caccgatgtc cagtgtagtc
     6781 aatattctag cgggaatcat gatgggacct gtttatgcct tggtcatggc tacagtcaca
     6841 gcctttatcc gtatgacgac tcaagggatt ccgcctttag ctctcacagg agcgactttt
     6901 ggagcccttc tggcaggtct cttttataag tacggtcgaa aattttattt ttctgccttg
     6961 ggagaaattg tgggaacagg tattattggt tccattgttt cctatcctgt tatggtactc
     7021 tttacaggat cagctgctaa gcttagctgg tttatctaca cgcctcgatt tttcggagca
     7081 accttgattg gtacagcgat ttcctttatt gcatttcgat ttttaatcaa gcaggaattc
     7141 tttaaaaaag tgcagggata tttctttagt gaaaggatag actgatgcag gaatttacaa
     7201 atccctttcc tataggctct agttccctaa ttcactgtat gaccaatgag atttcttgtg
     7261 agatgctggc aaatgggatt ttggctctgg gatgcaaacc tgtcatggca gatgatcccc
     7321 gtgaggttct tgattttact aagcaaagtc aggctctctt catcaatttg gggcatttgt
     7381 cagctgagaa ggaaaaagca atccgcatgg cagcttcgta tgcaaaccaa tcttctctcc
     7441 cgatggtagt agatgcggtt ggcgtaacga cttcatccat tcgtaagagc ttagttaaag
     7501 accttttaga ctatagacct acggtcctta aaggaaacat gtcagaaatt cgaagtcttg
     7561 ttggattaaa gcaccacggc gttggggtcg atgcgagtgc taaagatcaa gaaacggagg
     7621 atttgcttca agtcttgaaa gactggtgtc agacctatcc tggtatgtca ttcttagtta
     7681 ctggccccaa ggacctcatc gtttcgaaaa atcaggtcgc tgtactggag aatggctgta
     7741 ctgaattaga ctggataaca gggacaggag atttggttgg agccttaaca gccgtttttc
     7801 tcagccaagg aaagactggt tttgaagctt cttgcttagc agtctcttat ctcaatatcg
     7861 ctgctgagaa aatagttgtt caaggaatgg gattggaaga atttcgttac caagtactca
     7921 atcagctttc gctcctaaga agagatgaaa attggctaga taccatcaaa ggagaggttt
     7981 atgaatagag aagcacttag actatatctg gtaaccaatc gctatcaaga ttccgtggaa
     8041 agctttcttg caaaagttga gacggcctgc cgttcagggg ttaccatagt ccaattgcga
     8101 gaaaaaaatc tcacaaccaa tcaatattat caactggcaa aacaagtcaa ggaaataaca
     8161 gatgcttatc aggtaccctt gataatcgat gatcggttgg atgtttgtct tgcggttgat
     8221 gctgctggtc tgcatattgg agatgacgaa ctcccagttt cggttgcccg acaagtcttg
     8281 ggtcctgaaa aaatcctcgg tgtcaccgct aaaacggtta aaagagctct ggaagcggag
     8341 aaatcaggtg cagattactt ggggacagga gccatttttc cgacaaccac caaggaaaat
     8401 gcacccatca ccctgatttc aaccttgaaa acaatttgcc aaacggttgc cattccaata
     8461 gttgctattg gcggcttgac ttcggagaat attgatcagc ttatgggcac tggcatagct
     8521 ggtgtagctg ttgtacgtga tttgatgcaa gcagaagata ttgaggcaaa aacgcaagcc
     8581 tttttaacaa agttgcatga cattctttcc taattaatac tcaatgaaaa tcaaagagta
     8641 aactagaaag ctagtcgcag gttgctcaaa gtactgcctt gaggttttag ataagactga
     8701 cgaagtcagc tcaaaacagt gttttgaagt tgcagatgga agctgacgta gtttgaagag
     8761 attttcgaag agtataaaaa ctctctaatt cccttaaagt gggaactaga gagttttttt
     8821 cttaatcttt aaaagttgta tggttgactg gaccagaacc atgaccaagt tgaggggcat
     8881 cttgaatagc ttttgtgata aaggccttgg ccttatcaac tgcctggtaa agactcttgc
     8941 ccttggctag ttcagcagta atcactgcag caaaggtaca tccagtacca tgggtgtgac
     9001 aggtttgaat tcgtgggctt tcccagacaa attgttcatt cttggtaaag aggaaatctt
     9061 tagcaccacc tttgagatgt ccgcctttga gatgtccgcc tttgataacc acagactgag
     9121 gaccaaattc ttttaaaatc aggcgaccag cacgctgcat gtcttcgggg tcatggattg
     9181 aaaaaccaac aatctcttct gcttcaggaa gatttggcgt aataatagtt gctagaggta
     9241 gtaagtttgt tttgagatag tctctagcat ttgagtcaat caaggcatct ccacttgtag
     9301 caaccataac aggatcaagg acatagggac aatccagttt tttaagatag ggttggatga
     9361 tttccatgat ttcagtagta gccaacattc cagtttttac agcctgaggt ggaatatcag
     9421 aaaagacact gtccaattgg gctttcaaca tttgaggaga aacgtgctcg attagctgaa
     9481 cacctctggt attttgagcg acaagactgg ttacaacagc cattccatag acatctctcg
     9541 cttggaatga ctttaaatct gccataatgc cagcaccacc actagggtca gttcctgcaa
     9601 tggtcaaagc aacgggtaaa taagtcatct aaaacctccc aaccaactga agtttgtatt
     9661 cttaagaaca agctagtctg ttttagaaag gcaatagaag actgctagtc cccattatca
     9721 tttccacttc catcagctag cattacctag attgagtcag agggatctaa cagtcgttaa
     9781 tctcagcttt acgcacccct agtggttgtt ttcttttttc tttagtatag ca
//
LOCUS       DAVKZB010000066         9768 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKZB010000066 DAVKZB010000000
VERSION     DAVKZB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.066"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            7..6042
                     /locus_tag="VXA80_001415"
     CDS             7..6042
                     /locus_tag="VXA80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0353899.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALRILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYHEETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKPKEQGPEKTEEVPVK
                     PTEETPVNPNEGATEGTSIQGAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPENNDSKNENSEKTGEEVPVNPNEGTVEGTSNQETENPVQPAEESTTNS
                     EKVSPDTSSENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEP
                     SNGNSTEDVSTKSNTSNSNGNEEIKQENELDPDKKVEDPEKTLELRNVSDLELYSLSN
                     GTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKL
                     QQEIESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVEL
                     STDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFESLRGATIEKLSLKNVSISGKDD
                     IGSLANEAQNNTKIKQVHVDGVLAGERGIGGLLAKADQSSITESSFKGRIVNTYETTA
                     SYNIGGLVGHLTGSKASLTKSKATVVISSNTNSSDQTVGGIAGLVDKDAHIQNSYSEG
                     DINNSQRFGKVAGIAGNLWDRESNSENHAGRLTNVLSDVNVTNGNAISGYHYNGMKIT
                     DAFSNKANKVFNVTLEKDEVVSKESFEERGTMLEASQIASKKAEINLLTPPIVEPLST
                     SGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVKENSILYQKELLSA
                     VMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQDDFANLAEYSIGDSGLLYT
                     PNQFLYDQDSIINQVLPELQQVAYDSEAIRKTLGISPEVKQTELYMEDQFTKTKQDLA
                     NRANSLKKLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNV
                     KDLVLYHLDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFST
                     LEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLASTKYSIGVYDRITSD
                     SWKYRNMVLPLLTLPERSVFVISTISSLGFGAYDRYRNKEHQANGDLNSFVEKSAHET
                     AERQRDHYDYWYRILDEKGREKLYRNILLYDAYKFGTDHTEGKATEVADFDSPNPAMK
                     HFFGPVGNKVGHNGHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYG
                     RRSGLGPEFFAKGLLQAPDQPSDATITINSILKHKTSDSTEGQRLQVLDPTTRFNDAA
                     DLQNYVHNMFDVVYMLEYLEGQSIVKQLDAYQKMTALRKIENKYVKDPADGNEVYATN
                     VVKELTEEEARNLNSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAPIFSALSSEKG
                     TPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEGIAKQNGQTINLYGKKQGLVTDKLV
                     LEKIFSGKYPSWTAFKKAMYQERIAQFDHLTKVIFKDPTKPWPSYGTKTINNVDELQK
                     LMDEAVLQDATGTRWSNYNPETDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6094..6270
                     /locus_tag="VXA80_001416"
     CDS             6094..6270
                     /locus_tag="VXA80_001416"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0353900.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            6489..7340
                     /gene="ylqF"
                     /locus_tag="VXA80_001417"
     CDS             6489..7340
                     /gene="ylqF"
                     /locus_tag="VXA80_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV0353901.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7327..8124
                     /locus_tag="VXA80_001418"
     CDS             7327..8124
                     /locus_tag="VXA80_001418"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV0353902.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKSLVLGKKES"
     gene            8140..9690
                     /locus_tag="VXA80_001419"
     CDS             8140..9690
                     /locus_tag="VXA80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV0353903.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3339 a         1768 c         2001 g         2660 t
ORIGIN      
        1 ggaataatgg aaaagtattt tggtgaaaaa caagagcgtt tttcatttag aaaattatca
       61 gtaggacttg tatctgcaac gatttcaagt ttatttttta tgtctgtatt agctagttca
      121 tctgtggatg ctcaagaaac tgcgggagtt cactataaat atgtggcaga ttcagagcta
      181 tcatcagaag aaaagaagca gcttgtctat gatattccga catacgtgga gaatgatgat
      241 gaaacttatt atcttgttta taagttaaat tctcaaaatc aactggcgga attaccaaat
      301 actggaagca agaatgagag gcaagcccta gttgctggtg ctagcttagc tgctctgaga
      361 attttaattt ttgctgtttc caagaaaaag gttaagaata aaacggtatt acatttagta
      421 ttggttgcgg gaatgggaaa tggtgtctta gtttcagtcc atgctttaga aaatcatctt
      481 ttgctaaatt acaatacgga ctatgaattg acctctggag aaaaattacc tcttcctaaa
      541 gagatttcag gttacactta tattggatat atcaaagagg gaaaaacgac ttctgatttt
      601 gaagtaagta atcaagaaaa atcagcagcc actcctacaa aacaacaaaa ggtggattat
      661 aatgttacac caaattttgt agaccatcca tcaacagtac aagctattca ggaacaaaca
      721 cctgtttctt caactaagcc gacagaagtt caagtagttg aaaaaccttt ctctactgaa
      781 ttaatcaatc caagaaaaga agagaaacaa tcttcagatt ctcaagaaca attagccgaa
      841 cataagaatc tagaaacgaa gaaagaggag aagatttctc caaaagaaaa gactggggta
      901 aatacattaa atccacagga tgaagtttta tcaggtcaat tgaacaaacc tgaactctta
      961 tatcatgagg aaactataga gacaaaaata gattttcaag aagaaattca agaaaatcct
     1021 gatttagctg aaggaactgt aagagtaaaa caagaaggta aattaggtaa gaaagttgaa
     1081 atcgtcagaa tattctctgt aaacaaggaa gaagtttcgc gagaaattgt ttcaacttca
     1141 acgactgcgc ctagtccaag aatagtcgaa aaaggtacta aaaaaactca agttataaag
     1201 gaacaacctg agactggtgt agaacataag gacgtacagt ctggagctat tgttgaaccc
     1261 gcaattcagc ctgagttgcc cgaagctgta gtaagtgaca aaggcgaacc agaagttcaa
     1321 cctacattac ccgaagcagt tgtgaccgac aaaggtgaga ctgaggttca accagagtcg
     1381 ccagatactg tggtaagtga taaaggtgaa ccagagcagg tagccccatt gccagaatat
     1441 aaggggaata ttgagcaagt aaaacctgaa actccggttg agaagccgaa agaacaaggt
     1501 ccagaaaaaa ctgaagaagt tccagtaaaa ccaacagaag aaacaccagt aaatccaaat
     1561 gaaggtgcta cagaaggaac ctcaattcaa ggagcagaaa atccagttca acctgcagaa
     1621 gaatcaacaa cgaattcaga gaaagtatca ccagatacat ctagcgaaaa tactggggaa
     1681 gtgtccagta atcctagtga ttcgacaacc tcagttggag aatcaaataa accagaaaat
     1741 aatgactcta aaaatgaaaa ttcagaaaaa actggagaag aagttccagt aaatccaaat
     1801 gaaggcacag tagaaggtac ctcaaatcaa gaaacagaaa atccagttca acctgcagaa
     1861 gaatcaacaa cgaattcaga gaaagtatca ccagatacat ctagcgaaaa tactggggaa
     1921 gtatccaata aacctagtga ttcaaaacca ccagttgaag aatcaaatca accagaaaaa
     1981 aacggaactg caacaaaacc agaaaattca ggtaatacaa catcagagaa tggacaaaca
     2041 gaaccagaac catcaaacgg aaattcaact gaggatgttt caaccaaatc aaacacatcc
     2101 aattcaaatg gaaacgaaga aattaaacaa gaaaatgaac tagaccccga taaaaaggta
     2161 gaagatccag agaaaacact tgaattaaga aatgtttccg acctagagtt atacagtttg
     2221 tcaaatggta cttataaaca acacatttcg ttagaacaag ttccaagcaa tccaaatagc
     2281 tactttgtta aagtgaaatc ttcttcattc aaagatgtat acctaccagt agcatcaata
     2341 tcagaggaaa gaaaaaatga taaaatcctt tataaaatca cagcaaaagt agagaagctt
     2401 cagcaggaga tagaaagcag atataaagat aattttacct tctatctagc taagaaggga
     2461 acagaagaaa caacaaactt tacttccttt agtaatctgg tcaaagctat aaaccaaaat
     2521 ctctctggaa cctatcattt aggagctagc ctgaatgcta acgaagtgga gcttagcact
     2581 gatgataaat cctatatcaa gggaaccttt actggtcagt tgattggtga aaaagatggt
     2641 aagcattatg ctatttacaa cttgaaaaag cctctttttg aaagcttgag aggggccaca
     2701 atagaaaagt tgagtctgaa aaatgtctct atttcaggga aagatgatat cggttcactg
     2761 gcaaatgaag ctcagaataa cacaaaaatt aagcaagttc atgtggatgg tgtacttgcc
     2821 ggtgaacgtg gtatcggtgg tttgttggct aaggcagatc aatcaagcat cacagagagt
     2881 agtttcaagg gaagaattgt caatacctat gaaacaactg cttcctataa tatcggtggc
     2941 ctggtcggtc atttgacagg aagcaaagct tcactgacta agtcaaaagc gacagtggtc
     3001 atttcatcaa atacaaatag ttctgaccag accgttggag gtatcgctgg tcttgtagac
     3061 aaagatgcgc atattcagaa tagctattct gaaggggata ttaataatag ccaacgtttt
     3121 ggaaaagttg ctggtatagc aggtaactta tgggatcgtg aatcaaatag tgaaaatcat
     3181 gctggaagat tgactaatgt gcttagtgat gtcaatgtaa ctaatggaaa tgccattagt
     3241 ggttaccatt ataatggaat gaaaattact gatgcattta gtaataaagc gaataaagtt
     3301 ttcaatgtca ctttagagaa ggatgaggtc gttagcaaag aatcctttga ggaaagagga
     3361 acaatgctag aggcttctca aattgcaagc aaaaaagcag aaatcaatct tctcactcca
     3421 ccaatagtgg aacccctctc aacaagcggt aagaaagaca gtgatttttc taagatagcc
     3481 cattatcaag ccaaccgtgc tttggtttat aagaacattg aaaaattgtt acctttctat
     3541 aacaaggcga ccatcgtgaa atacggaaac ctggttaagg agaacagtat cttatatcaa
     3601 aaagaactct tgtctgcagt tatgatgaag gatgaccaag taatcacaga tattatttcc
     3661 aacaaacaga ctgcaaacaa actcttgctt cactacaagg accattcatc tgaaaagttt
     3721 gatctcaggt accaggatga ttttgccaat ttggcagaat atagcattgg agatagtggg
     3781 cttctctata cgccaaatca attcttgtat gaccaagact ctatcatcaa tcaagtttta
     3841 cctgaattac agcaggttgc ctacgattca gaagctatca gaaagacact cggaatttct
     3901 ccagaggtca agcaaactga gctctatatg gaagaccagt ttaccaaaac aaagcaagat
     3961 ttggcaaaca gggcaaacag tttgaaaaaa ctcttgtctg cggatgctgg actggctagt
     4021 gctaacccag tcactgaagg ttatcttgta gataaaatca aacgcaacaa ggaagccttg
     4081 ctacttggct tgacctatct ggaacggtgg tataacttta gctatggtca ggtgaatgtc
     4141 aaagacctag ttctgtacca tttggacttc tttggtaagg ggaatgcttc accattagat
     4201 actctgattg agttgggtaa atctggcttt aacaatcttc tagctaagaa taatgtcgat
     4261 acttatggta tcagtcttgc cagtcaacat ggaacgacag atttgtttag cacgctggaa
     4321 cattaccgaa aagtcttttt accaaataca agcaataatg actggtttaa atcagagact
     4381 aaggcttaca ttgtcgaaga aaaatccact atcgaagagg tgaaaacgaa gcaagggtta
     4441 gctagcacca agtattctat cggtgtttat gatcgtatca ctagcgacag ctggaaatac
     4501 cgtaatatgg tactacctct acttaccttg ccagaaagat ccgtatttgt catttcaacc
     4561 atatctagtc taggttttgg tgcttatgat cgctatcgta ataaagaaca tcaagcaaat
     4621 ggtgatctta atagctttgt tgaaaagagt gctcacgaaa cagcagagcg tcaacgagat
     4681 cactatgatt attggtatcg tattctagat gaaaaagggc gtgaaaagct ttatcgaaat
     4741 atcttgcttt atgatgccta taaatttgga actgatcata ctgaaggaaa ggctacagaa
     4801 gtagcagatt ttgatagtcc aaatcctgca atgaaacatt tctttggacc tgttggaaat
     4861 aaggttggac ataatggaca tggtgcttat gctacagggg atgccgttta ttatatgggt
     4921 tatcgtatgt tggacaagga cggcgccatt acttatacgc atgagatgac acatgactca
     4981 gatcaggaca tttatcttgg aggttatgga agaagaagtg gacttggacc tgagttcttt
     5041 gctaaaggct tactacaagc accagaccaa ccaagtgatg caaccattac catcaactct
     5101 atcttgaaac ataaaacatc agatagtaca gaaggtcagc gattgcaagt acttgatcca
     5161 actacaagat ttaatgacgc agcagatctt cagaactacg tccacaatat gtttgatgtc
     5221 gtttacatgt tggaatatct cgaagggcaa tctatcgtga aacagttaga tgcttatcag
     5281 aaaatgacgg ccctgagaaa aatcgagaat aaatacgtga aagatcctgc agatggaaat
     5341 gaggtttacg ctactaacgt agtcaaagaa ttgacagaag aagaggcccg aaacctgaat
     5401 agttttgata gtttgattga caataatatt ctttcagctc gtgagtataa ggctggtaca
     5461 tatgagagaa atggctactt tactataaaa ctctttgcac caatcttttc agcattgagc
     5521 agtgagaaag gtactcctgg agatcttatg ggacgaagga tagcttatga actcttagct
     5581 gctaaaggat ttaaagatgg tatggtacca tatatttcca accaatatga aggaattgct
     5641 aaacaaaatg gtcagacaat caacctttac ggtaaaaaac aaggattagt gacagataag
     5701 cttgttttgg aaaagatatt ctctggcaag tatccatctt ggacagcctt taaaaaagct
     5761 atgtatcaag agcgtattgc tcagtttgat catttgacta aagttatttt taaagatccg
     5821 acaaaaccat ggccaagcta tggcacaaag actatcaata atgtggatga attgcagaaa
     5881 ctaatggatg aagctgtttt gcaggatgct acaggtacac gttggagtaa ttataatcca
     5941 gaaacggaca gtgccgtcca taagttgaag agagcaatct ttaaagccta tcttgaccaa
     6001 acaaatgatt ttagaagttc aatttttgag aataaaaaat agtgtttact attaggaaat
     6061 aaagtttaaa aaggtgatga agaacaaacc aagattcaag caggaattcc tactgataat
     6121 gaagtaagtt atgctcttat ttatcagcag gaaactcttc ctgcaacagg ttcatcaact
     6181 tctgcactta cagctttagg cctattagct gttggtagtt tagttctttt ggttcataat
     6241 atgacgggaa cagttttttg ctccctctga aaagtcatca tttgttggct tttttctata
     6301 taggctaaaa gatagggtaa aaggctatca tcggacaaaa taaagaaggc atgatataat
     6361 ataaagtaga tttctatgtc ataaaacaag aactgtttgg acatcattca tttgaaaact
     6421 ctctatgttc aaactatagt aaaataaaat aggggatcta aatccttgct acgaaaggaa
     6481 aaaactcaat ggctactatt caatggtttc ctggtcacat gtctaaagcg cgtcgacagg
     6541 tgcaggaaaa tttaaaattt gttgattttg tgacgatttt agtagatgca cgcttgcctc
     6601 tatctagtca aaatcctatg ttaaccaaga ttgttggtga taaaccaaaa ctcttgattt
     6661 taaacaaggc cgacttggct gatccagcaa tgaccaagga atggcgtcag tattttgaat
     6721 cacaaggaat ccagacgcta gctatcaact ccaaagagca agtgactgta aaagttgtaa
     6781 cagatgcggc caagaagctc atggctgata agattgctcg ccagaaagaa cgtgggattc
     6841 agattgaaac cttgcgtacc atgattattg ggattccaaa cgctggtaaa tcaactctga
     6901 tgaaccgttt ggctggtaaa aagattgctg ttgttggaaa caagccaggg gtcacaaaag
     6961 gtcaacaatg gcttaaaacc aataaagatc tggaaatctt ggatacaccg gggattctct
     7021 ggcctaagtt tgaggatgaa actgttgcac ttaagttggc attgactgga gctatcaagg
     7081 atcagttgct tcctatggat gaggttacca tttttggtat caattatttc aaagaacatt
     7141 atccagaaaa gctggctgaa cgcttcaaac aaatgaaaat tgaagaagaa gcgcctgtga
     7201 ttattatgga tatgacccgc gccctcggtt tccgtgatga ctatgaccgt ttttacagtc
     7261 tcttcgtgaa ggaagtccgt gatggcaaac tcggtaacta taccttagat acattggaag
     7321 acctcgatgg caacgattaa agaaatcaaa gaactccttg tgacagtcaa ggagttagaa
     7381 agccctattt ttttagagct tgaaaaggat aatcgctcag gagttcaaaa ggaaatcagc
     7441 aagcgtaaaa gagccattca agctgaatta gatgaaaatt tgcgcttgga atccatgctt
     7501 tcttatgaaa aagaacttta taagcaagga ttgaccttaa ttgcaggtat tgatgaggtt
     7561 ggtcgtggtc ctcttgctgg tcctgtagtc gctgcggccg ttattttacc taaaaattgt
     7621 aagattaaag gtctcaacga cagcaagaaa attcctaaaa agaaacatct ggagattttc
     7681 caagccgttc aagaccaagc cttgtcgatt ggaattggta tcatagataa tcaggtcatc
     7741 gaccaagtca acatctatga agcaaccaaa ctagccatgc aagaagcaat ctcccagctc
     7801 agccctcaac cagagcacct tttgattgat gccatgaaac tggacttgcc catttcacaa
     7861 acctccatta tcaaaggaga tgccaactcc ctctctatcg cagcagcatc tatagtagcc
     7921 aaggtaacac gtgatgaatt gatgaaagaa tacgatcagc agttctctgg ctatgatttc
     7981 gctactaatg caggatatgg cacagctaaa catctggaag gcctcacaaa actaggagtt
     8041 accccaattc accgaaccag ctttgaaccc gttaaatcac tggttttagg taaatcactg
     8101 gttttaggta aaaaagaaag ttaattgaaa ggaaatacca tggaggaaca gtcggaaata
     8161 gtccgttcta agaaagaatt cgcctttgca tccagcacta tactatccca agttggtcga
     8221 ggaatcattg tcggcctcat cgttggaatt atcgtcggat cctttcgttt cttaattgaa
     8281 aagggcttcc acctgataca aggagtttat caagatcaag ggtacttagt gcgcaatctt
     8341 tttgtactgg ttttgtttta tatactcatc tgttggctca gtgccaaact aacacggtca
     8401 gaaaaagata ttaaaggctc aggaattcct caagtcgaag ccgaactgaa aggcctcatg
     8461 tccctcaact ggtggggcat tctttggaaa aaatatgtgc taggtattct tgctattgcc
     8521 agtggactca tgctgggtcg agagggaccc agcattcaac ttggagcagt tggtggtaaa
     8581 ggaattgcca agtggctcaa atccagtcca gtagaggaac gttccttgat tgccagtgga
     8641 gctgcagcag gtttagccgc agcctttaat gctcctattg cagcacttct ctttgttgta
     8701 gaagaagtct atcaccattt ttcgcgcttt ttctgggtct caactctagc agccagcatc
     8761 gtagcaaact ttgtgtctct actcatgttc ggtttgacac cagtattgga tatgccagat
     8821 aacattcctc ccatgaccct agaccagtat tggatatatc tcgtcatggg aattttcctt
     8881 ggattttcag gttttctcta tgagaaagct gtattaaacg ttggaagagt ttatgacttg
     8941 attggtcaaa aaatccattt ggatagggct tattatccca tcttggcttt tatccttatc
     9001 ataccagtcg gaatcttctt acctcaaatc attggtggcg gaaatcagct tgtcctttct
     9061 ttaactgaac aaaattttag tttccaagtt ttattagctt actttttaat ccgctttatt
     9121 tggagtatga ttagctatgg aagtggactg ccaggaggaa ttttcctccc cattttagct
     9181 cttggttctt tgcttggtgc cttagttggt gttatctgtg tcaatcttgg acttgtcagt
     9241 caagagcaat tccctatatt tgtcattcta ggaatgagtg gctattttgg agccatatca
     9301 aaagctccct taaccgctat gatcctcgta actgagatgg taggagatat tcgcaacctt
     9361 atgccacttg gtcttgtcac tcttgtttct tatattatca tggatttgct caaaggtacg
     9421 ccagtctatg aagccatgct ggaaaaaatg cttccagaag aagtatctag cgaaggagaa
     9481 gttacactta tcgaaatacc agtttctgat aaaattgctg ggaaacaagt tcatgaactc
     9541 aacttaccac acaacgtcct catcacaact caagtccata atggcaagag ccaaacagtt
     9601 aacggctcaa ccagaatgta tctgggtgat atgattcacc tggttattcc aaaaagtgaa
     9661 attggaaaag tcaaagattt gttgttgtag tatgatcatt tacataattt atgctatgta
     9721 aatgatcagt ttgatttatt tagaaaaccg attctcagaa atgagatc
//
LOCUS       DAVKZB010000067         9737 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKZB010000067 DAVKZB010000000
VERSION     DAVKZB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.067"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            248..343
                     /locus_tag="VXA80_001420"
                     /pseudo
     CDS             248..343
                     /locus_tag="VXA80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(596..1216)
                     /locus_tag="VXA80_001421"
     CDS             complement(596..1216)
                     /locus_tag="VXA80_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0353904.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1216..1698)
                     /locus_tag="VXA80_001422"
     CDS             complement(1216..1698)
                     /locus_tag="VXA80_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV0353905.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKYLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1698..2540)
                     /locus_tag="VXA80_001423"
     CDS             complement(1698..2540)
                     /locus_tag="VXA80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV0353906.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2635..3435)
                     /locus_tag="VXA80_001424"
     CDS             complement(2635..3435)
                     /locus_tag="VXA80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV0353907.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKKETFRKLFLKP"
     gene            3579..4958
                     /gene="glmU"
                     /locus_tag="VXA80_001425"
     CDS             3579..4958
                     /gene="glmU"
                     /locus_tag="VXA80_001425"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV0353908.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4968..5513
                     /locus_tag="VXA80_001426"
     CDS             4968..5513
                     /locus_tag="VXA80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0353909.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5525..5839
                     /locus_tag="VXA80_001427"
     CDS             5525..5839
                     /locus_tag="VXA80_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV0353910.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5856..6548
                     /locus_tag="VXA80_001428"
     CDS             5856..6548
                     /locus_tag="VXA80_001428"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV0353911.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGTVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6625..7116
                     /gene="rocS"
                     /locus_tag="VXA80_001429"
     CDS             6625..7116
                     /gene="rocS"
                     /locus_tag="VXA80_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV0353912.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7214..7804
                     /locus_tag="VXA80_001430"
     CDS             7214..7804
                     /locus_tag="VXA80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV0353913.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7813..9576
                     /locus_tag="VXA80_001431"
     CDS             7813..9576
                     /locus_tag="VXA80_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV0353914.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPSLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         2999 a         1692 c         2084 g         2962 t
ORIGIN      
        1 tgtctaactt ttggggtgta gttcatgtac acctgatatg atgcatttta taattttaaa
       61 gactttttga ctagcctcat ttttttaact tgatactcag tgaaaagcaa agattaaact
      121 aggaagctag ctgtaggctg ctcaaagaac agctttgagg ttgcagataa aacttgtgag
      181 gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga agggtatgag
      241 tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag agttcttatg
      301 tgaagagttt gggcataaac ttttaccttt tcctccctac tcatcttagt atagaaaagt
      361 gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt taaattctca
      421 aatcatatta ttcagttctt atttcatttt gctctacaat cctgttgaga agacacgtgt
      481 tcatatcaaa aaggtattgg caagttgcaa taccttttta cgaggctctg ttgtcttatt
      541 tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc tataattatg
      601 attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca tgggtttcta
      661 taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt tctttatagc
      721 ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt agaatacgct
      781 cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt gtactataga
      841 gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga aaagcctgga
      901 tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac ttcccaagtt
      961 gccattcacg gagattagga acgatttcta aagaacaggg ggtatggagt tgactttgga
     1021 taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga tcaaaaggaa
     1081 tttccttgag atactgacca agtcgtttta gggtttcaat ggattcagga agaagaggag
     1141 aatcaccact agcaccttga aaacgacctt cttggttcca gagggtacga ccgtggcgga
     1201 caaagtagag tttcattact tgatgtcctc caaaatatct acaaagtctg cctttacaaa
     1261 gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg cagagacaat
     1321 catttgatcc aaatctagag caattttatc gataaaaatg ggataattgt gtttctgacg
     1381 aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta agtacttttg
     1441 tggaatcatg ctcacttttt tattgccaga aattttagct agtttctttt cagacaagtg
     1501 ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa gcgccaaggt
     1561 tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg cattgatttg
     1621 aatcccctga tgagggatag ctgctttaga taggatttgt tctaccaatg tttttttgat
     1681 tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca aataccaagc
     1741 cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga aagtcctgtg
     1801 tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt acttgatagg
     1861 acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc ataatccatc
     1921 aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa gaaggttccc
     1981 aagagatcaa aagttttaaa accagtcaac ataggagcat agagtcccaa aatcaaaagt
     2041 aaattcgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc gcctgctagg
     2101 acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc atagagtcct
     2161 aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc tcggtcagat
     2221 tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc cgcaaagctc
     2281 cataatacaa agacctgata attttcaagc aggtattcaa tcgggtagga aaataagccg
     2341 attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc tttaaggtct
     2401 ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat tgctgctaga
     2461 acccctccgg aaattcccgg taggataaat ccaagagcaa ttacaatccc tttaataacg
     2521 cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa aatccttact
     2581 atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat gcttttaagg
     2641 ttttaaaaaa agctttcgaa aggtttcttt tttatttttt aagggagaga taacgttgat
     2701 atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt gaatatcata
     2761 atctaaatca gttttaggac tgctctccaa aaatcctgag tctgagccgt aggacggaat
     2821 ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt agacaccaac
     2881 gtagatgccg atgtttttag cacctagtga tgctagtttt gctcgaaagt tttcgacacc
     2941 ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac tagggctgta
     3001 aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttgga cactttttcc
     3061 agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag tgatatgact
     3121 cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg tcgtttgagc
     3181 accactgtga acacgaacaa tagcacctga aatattttgt gagagggcgt cgtagttgat
     3241 ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt gtttcaaagc
     3301 ctgtgcttca atctgtgcta tattggattt tgttttaaac gattggctgt cattaagtgg
     3361 gcgattgatg attaaaatga acatcataat cccaaaaaaa ctaaataaaa taagtggatg
     3421 aatttgtttt ctcatatctt ataattctac cctaaaaatc aaaaaaaatc aaaaaaatgg
     3481 gttaaggaag agactttaga gcattttttc attcaagagt gcggaatgat ttgaaatatg
     3541 gtataataaa agggaatttc tacagaaaag agaagattat gtcaaatttt gccattattt
     3601 tagcagcggg taaagggact cgcatgaaat ctgatttgcc aaaagttttg cacaaggttg
     3661 cgggtatttc tatgttggaa catgttttcc gtagtgtggg agctatccaa cctgaaaaga
     3721 cagtaacagt tgtaggacac aaggcagaat tggttgagga ggtcttggct ggacagacag
     3781 aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc agttatgatg acagagccta
     3841 tcttagaagg tttgtcagga cacaccttgg tcattgcagg agatactcct ttaatcactg
     3901 gtgaaagctt gaaaaacttg attgatttcc atatcaatca taaaaatgtg gccactatct
     3961 tgactgctga aacggataat ccttttggct atggacgaat tgttcgtaat gacaatgctg
     4021 aggttcttcg tattgttgag cagaaggatg ctacagattt tgaaaagcaa atcaaggaaa
     4081 tcaacactgg aacatacgtc tttgacaacg agcgtttgtt tgaggctttg aaaaatatca
     4141 ataccaataa cgctcaaggc gaatactata ttacagacgt cattggtatt ttccgtgaaa
     4201 ctggtgaaaa agttggcgct tatactttga aagattttga tgaaagtctt ggggtaaatg
     4261 accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat cataaacaca
     4321 tggtcaatgg tgttagcttt gtcaatccag aagcaactta tatcgatatt gatgttgaga
     4381 ttgctccgga agttcaaatc gaagccaatg ttaccttgaa agggcaaacg aaaattggtg
     4441 ctgagactgt tttgacaaac ggtacttatg tagtggacag cactatcgga gcaggagcgg
     4501 tcattaccaa ttctatgatt gaggaaagta gtgttgcaga cggtgtgaca gtcggtcctt
     4561 atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt aactttgttg
     4621 aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg tcatttgact tatatcggaa
     4681 actgtgaagt gggaagcaac gttaatttcg gtgctggaac tattacagtc aactatgacg
     4741 gcaaaaacaa atacaagaca gtcattggag acaatgtctt tgttggttca aattcaacca
     4801 ttattgcacc agtagaactt ggtgacaatt ccctcgttgg tgctggttca actattacta
     4861 aagacgtgcc agcagatgct attgctattg gtcgcggtcg tcagatcaat aaagacgaat
     4921 atgcaacacg tcttcctcat catcctaaga accagtagga gcctatcatg gagtttgaag
     4981 aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat atttaaactg gtccaagatc
     5041 aggttgaatt accagaaggc aagggaactg cccgacggga tttgattttc cacaatgggg
     5101 ctgtctgtgt tttagcagta acggatgaac aaaaacttat cttggtcaag cagtaccgca
     5161 aagctatcga ggctgtctct tacgaaattc cagccggaaa attggaagta ggagaaaaca
     5221 cagcccctgt ggcagctgcc cttcgtgaat tagaggaaga aacagcctat acagggaaat
     5281 tagaactctt gtacgatttt tattcagcta ttggcttttg taatgagaag ttaaaactat
     5341 atttagcaag cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag gatgaaacct
     5401 tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt aatccaatca ggtcatatct
     5461 gtgatgccaa gacaattatg gctgttcagt attgggagtt gcagaaaaaa tagaggaggt
     5521 cagtatgggt aaatctttat taacggatga aatgattgaa agagctaata gaggcgaaaa
     5581 aatttcaggt cctcctttgc tagatgataa tgaggaaact aagattttac caacctcttc
     5641 ttcccgtttt ggttatgcca atcctaagga tcatggtttt agccaggaaa ccttgaagat
     5701 tcaggtcgaa ccatctattc ataaaagccg tcgcattgaa aataccaaga gaaatgtctt
     5761 caattctaag ttgaataaaa tcttattcgc ggtcatcttt ctcttgattt tgcttgtttt
     5821 agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt attgctgcta
     5881 tgccagaaga actggcttat ctggtccagc atttagacaa tgcccaggag caagttgttt
     5941 tggggaatac ctatcataca ggaaccattg cttctcatga agtcgttctt gtagaaagtg
     6001 gaattggtaa ggtcatgtct gctatgagtg tggcgatttt ggctgatcat ttccaggtgg
     6061 atgcccttat taatacgggt tcagctggga cagtagcaga aggtatcgct gttggggatg
     6121 tcgtgattgc tgacaaatta gcctatcatg acgtggatgt cacagctttt ggctatgctt
     6181 atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt gttgctcaaa
     6241 tccaagagag tttatctcaa ttggaccaaa actggcatct tggtttgatt gctacaggag
     6301 atagttttgt tgcaggaaat gacaagatag aagcgattaa gtcccatttc ccagaagttt
     6361 tagccgtgga gatggagggg gcagctattg ctcaagcagc gcatgccctc aatctcccag
     6421 tcttagtcat cagagctatg agtgacaatg ccaaccatga agcaaacatc ttttttgatg
     6481 agtttattat cgaagctgga cgtcgctctg cccaagtctt gttgaccttt ttgaaggctt
     6541 tagattaagc ggaaatttga cagtttttct agcttatgat aagatttaag taaagaaaag
     6601 ctagaaaacg tttcagagga tattatgagt attgaaatga ccgtcagtga gattgcagag
     6661 gtcttaggat tatctcgcca agcaatcaat aaccgtgtca aagaattacc agaagaagac
     6721 acagataaaa atgacaaagg ggtaacagtt gttaccagaa gtggcttgat taagctagaa
     6781 gaaatctata aaaaaacgat ttttgaagat gagcctgtca gtgaagatgt caaacaacgt
     6841 gaactgatgg agattctagt ggatgagaag aatgcagaaa ttcttcgtct ttatgaacaa
     6901 ttaaaagcca aggatcgtca gttatcagaa aaagacgagc agatgcgtat caaagaccgt
     6961 cagattgctg agaaagacaa acaattagat cagcaacaac aattgaccct tcaagccatg
     7021 aaggatcaag aaaatcttaa gctagagctg gaccaagcaa aagaagaagt ccaatccact
     7081 aagaaaggct tttttgctcg tttatttgga ggataatcaa ggagctgttt aggttaaatg
     7141 ctaacctgat ggcttctttt gtttgtgaat agtatagttg attagaacag agacagtaga
     7201 ggagaggtac agaatggaac gattagaaga ttacattgct tttgatttag aatttaatca
     7261 gcacgaagga caaacacact tgattcaagt atcggcagtg cgttttagag atggtcggga
     7321 aatcgatgcc tatgattcct atgttcatac cagcgtccct ctaaaaagct ttatcaatgg
     7381 tttgaccggg attacagctg agaccttaaa agatgctcct ccagttgaga aagtcttaga
     7441 agaattccaa gagtttgtgg gttctttgct gatgataggc tacaatgctg ctaaaagtga
     7501 tttacctatt ttagcagagc atggtttaga ctatagccaa cagtacaagg tggatttgta
     7561 tgatgaagct tttgaacgtc ggagttcaga tttacatggt attgccaatc tcaaattgca
     7621 aactgttgcc tcatttcttg gatttcaagg tcagtctcat aatagtttag aggatgctcg
     7681 gatgacggcg cgtgtttacg aatccttctt agagtcggat caagctaggg aattgttaga
     7741 gacagaaagt agcttatcaa acaatccttt tggtggcttg gacttgtctc aattatttga
     7801 ctaggagtgc ctatgaaacc tgaaaaacct aaaaaattgg gtcttaaaca gatttacctc
     7861 aatcttgata aaatcctctt tctatttttc ttgattttta tgatagtgga gtatttgtgg
     7921 gtaccattca attcatggtt ggctagcttc ttattagatc agacaggtta tctttttatt
     7981 tcctataaca atgtcttagc catccttact cattctccat tgattagtct agctttttta
     8041 gccttgattg taattaacct tctggttgct tattttcaaa taggactcct ctttattggt
     8101 gctcgtcatc tcctctataa tgaaaagcga aggctaattg agtatatccg caaggttttc
     8161 catgaaagtt ttggatttat gaaaaagcta acttgcgcta aagccttgtt tatctttttc
     8221 tatatagcta tgctttttcc ttttatacgg aaaatgctga agatttatta tcttaataaa
     8281 atcgtcattc cagagtttat ccaagcttat ttagaagata aatattggat gtggtggctg
     8341 agcatcctac ttctatctct tatctttctc tatgtctctg tcagattgat gtttgccctt
     8401 ccgaagattg tatatgatca gctgactgtt cgagaagcag tgatgtttag cttggagaaa
     8461 accaagaaga gagttacttt ttatgcttgg aatttatttt acatcttgct caaagcacat
     8521 ttattatttt atcttccttt gattccttca ttattggctc aaactttggt agatgatatc
     8581 actcagagag agtctctgat tcttggtatt ttgaattttg tcgtgattaa aaatctctat
     8641 tatatggctc tgacttattt tttggttaaa tttgtttcat ttttgacagg aaaggagctg
     8701 gatatgcttc ctaggagaga aaaagatcac atcgtgcgat ggggtgtcat gacttgtgcc
     8761 agtcttttct ttgccttaga aggttatatt tatctggagg ctcccatggt tcatctacct
     8821 caacttattt ctcaccgagg ggtttccaat gcaaatggga ttcaaaatac agtagagtcc
     8881 ttggaaacta cagcacaact caaaccagac ttggtggaga cggacgtgca ggaaacaaaa
     8941 gatgggcagt ttgtcatgat gcatgatgct aacttgaaaa atctagcagg tatcaataaa
     9001 agtcctcaag acttaaactt ggaggagctt aaagggattg atatttttga aaatggctac
     9061 cagactaaaa tttcaagctt tgaagattat ctcagtcgag ccaacgaact tggtcaaaaa
     9121 ttactaattg aaattaaaac cagtaaaaaa gatagtccag acatgatgaa ccgcttttta
     9181 gcccgttatg ctgcaaagct caagatttat ggacatcaaa tccagtcttt agactaccat
     9241 gttgtcgaaa aagtaagaca gtatgatgcc gaactgccag tttatttcat catgccctac
     9301 aattctgtct ttcctaaaac aagagcgaca ggatatacta tggagtactc aaccttggat
     9361 gaatattttg taagcaaact atggacaacg gatcagaaac tttatgtctg gactgtcaat
     9421 gattcagaag ctatcagcaa atctcttcac ttaggagtag atggagtgat tacagatgat
     9481 ttggaaaaag ttcagcggga aatagaagta gcccaagaag atccagaata tacggatttg
     9541 ctcttgaaaa aagcattgga attctttgaa ttttagtaat aaaaaagagg aacggttgtt
     9601 cctcccatga taggtaagta ttcttgtgaa tgcttgcttt tttgtggttg caatccgttt
     9661 acgagaattg tttgtattgt tcttgtttca atttacttta tagtgctttg aagttggaat
     9721 gctacactat aacttct
//
LOCUS       DAVKZB010000068         9539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKZB010000068 DAVKZB010000000
VERSION     DAVKZB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.068"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            122..799
                     /locus_tag="VXA80_001432"
     CDS             122..799
                     /locus_tag="VXA80_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353915.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMAALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            811..1476
                     /locus_tag="VXA80_001433"
     CDS             811..1476
                     /locus_tag="VXA80_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0353916.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSALVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            1548..2774
                     /locus_tag="VXA80_001434"
     CDS             1548..2774
                     /locus_tag="VXA80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0353917.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(3260..3337)
                     /locus_tag="VXA80_001435"
                     /pseudo
     CDS             complement(3260..3337)
                     /locus_tag="VXA80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            3408..4064
                     /locus_tag="VXA80_001436"
     CDS             3408..4064
                     /locus_tag="VXA80_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV0353918.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            4054..4377
                     /locus_tag="VXA80_001437"
     CDS             4054..4377
                     /locus_tag="VXA80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV0353919.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            4481..5311
                     /locus_tag="VXA80_001438"
     CDS             4481..5311
                     /locus_tag="VXA80_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0353920.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            5465..6319
                     /locus_tag="VXA80_001439"
     CDS             5465..6319
                     /locus_tag="VXA80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0353921.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            6419..7792
                     /locus_tag="VXA80_001440"
     CDS             6419..7792
                     /locus_tag="VXA80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV0353922.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            7785..8846
                     /locus_tag="VXA80_001441"
     CDS             7785..8846
                     /locus_tag="VXA80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV0353923.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            8848..>9539
                     /locus_tag="VXA80_001442"
     CDS             8848..>9539
                     /locus_tag="VXA80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353924.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
BASE COUNT         2626 a         1826 c         2079 g         3008 t
ORIGIN      
        1 agtggctatc tatactttta gaagagtaca gactacaagc tgagagaatt ttgaaatttt
       61 aactttattc ctccagtcct tgcaattaga tataaaatag gctcttatag gagataatag
      121 gatgaagaaa atgaaagaag tgaaatttca cctagcaact ggcttgttga ttttgactta
      181 ttacctcatt tttaatgtca catcagactt agattttatg gctgctttgt ctgataatat
      241 gtactatgtg ttccaagttt tattggtact tattctggga actatagcta caattgcttt
      301 tgtcaaaagt gaacattgga aagagtgtgg tcgtttccag tttcgttggt cttacctggg
      361 tgttttcctt ctttcttttt tcttattgtt cgtgtgggca aatctaacaa cctatatctt
      421 tcctcgtacc cagaatggaa gtacagtcgt agaagtagcc accaatttaa caggaattag
      481 ctattttgtt acgcgaatac tttatactag tattatcgca cctgtatcag aagaggttgt
      541 ttgtcgtggt cttttgatga caagtttatc taaagttaag agatactatc ttgatgttct
      601 tgtttccgct gccatatttg gcgctatgca tgttttgcag tatggttgga taacgacaga
      661 tttcataaag tattttggaa tgggcttgat attttgtatg atgtttcgct acacccgatc
      721 catctattgg gctatagctc tgcatgcttc atggaatagt ttcttactca ttgttacttt
      781 attggtattt ggatattaga aaggtgatat atgaaaaaga taatctcaca ccgctacttt
      841 attatagttt ttctactagt tattgctgac cagaagttca gtgctctagt tttacgcagt
      901 gaccttgtta ctgggctatc tgactttgcc tattatctgt cggatatgat gttgaatttt
      961 cttgtggttt tatttgctct tattgctatg atttggtcgg ggaaatggca gaaaatcaat
     1021 agtagaaagt ttaaaggatc ctatcttttc tattccttcc tagctctgct tgcttttgtt
     1081 gcttggaatt ttgtcacctt ttttcttttc ccacctactc gaaatgaaat ttcttatcaa
     1141 catgctgctc ctacttttac aggagctacg gcatttttga tgtatttttt ttatcctgtg
     1201 attgcaggtc ccatttttga agacatgatt tatcgtggat tagtgatgac tgctctggaa
     1261 aaaggaaaga aatgggggct agatgtactt ggttccgctg ttttatttgg ggtctcgcac
     1321 attagtaatc acggttgggt cttgacagac tttgtcttct atatgggtgg aggtcttata
     1381 tttgcagtct tatttagaat gacaaagtca atttattggc ctattggact gcatatagtc
     1441 tacaatggca ttggtcagct tttgatgtta ctgtaatttt ggttgttagt agtatcgcgg
     1501 tctatccttg gggaaagatt cccatttgaa cttaagaagg agttgtgatg aaagtatttc
     1561 ttcaaaatag agattttagg caattaacta tcaaccagtg gatttcaacg cttggggata
     1621 cgatttttta tctggccttt ctgaattatg tggcagatgc gtcttttgca cctttggcga
     1681 ttttactcat tacgatttca gaaacccttc cccaaatttt acaaatcttt ctgggagttt
     1741 tggcagattt tcaacatcat cgtgtcttga aatacacagt cattagtttt gcaaaatttt
     1801 tgctttactc tatcgtttct ttatcacttt cagggcagtc tttctccttg ttgctagtag
     1861 catttatttg tctgcttaac ctcttgtctg acacattgag ttacttttca ggagccatgc
     1921 tcactccgat tttcattaga attattgggc aagaccatct ggcagaagct attggattta
     1981 aacagtcaac tgttagttta gtgaaaacaa tcagtaatat tctaggagga gtcttgctag
     2041 ggattctctc catccagttc atttccttgc tgaacgctct taccttttta atcgcatttt
     2101 taggtatcct cttcataaaa actgacctct tgaaagtaga aaaaacgatt aactatcaag
     2161 aaggactctc tgtaaaatcc ttttgccagc atttgctcca atcatcgaaa ttgatatgga
     2221 atatgaataa ggtgctcttg gttttgttta ttatctctac tagtcaagca gtgataaatg
     2281 ttacagttcc tgtctctact ctatttttga ggaatcaacc ctttttgaat ttacaaacag
     2341 gtcaatctct tgccttgcta tccacttttg aactgtcagc cctgattgtc ggaagccttg
     2401 taagtggcta tctgcaacat accatttcca taaaaacagc tctctatgcc tcacttgtca
     2461 tccagttgct tcttctagta ggatttgcca cagttcgttt tgactggatt ctcatcttta
     2521 gtaccttgga tgcctttttc gcaggtgttc tctctcctag attgcaagaa ctcgtcttta
     2581 aacaaatacc tgaggagtca atgggagcag ttcaatcctc tatcggtgcc attacggttg
     2641 ttttacctag cttatttaca atagctttgg taaccattgc tactagcttt ggaactctgg
     2701 cagttagctt tgttttattg ctatttcttc tagttgcctt tgttatgctt ctgaatattc
     2761 gtgaaagtat ttagtggaga atttctataa tatatgtttg agactggatt gccagtcttt
     2821 ttatgatggt caaaatcaac agactttttt ctcttttctg aatgtgttat aatagactga
     2881 atctaaaata gtacgaaaca attgctaaaa catttataga aattaatttt actttcccaa
     2941 tcgatttgtt ctcatcttat ttcaatctgc tatataatag tccatgctgt agctggattc
     3001 ttttcagcct acttatactc ttcgaaactc tcttcaaacc acgtcagctt cactttgccg
     3061 tatatatgtt actgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagctga
     3121 cttcgtcagt tctatccaca acctcaaaac ggtgttttga gctgacttcg tcagttctat
     3181 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tctatccaca acctcaaaac
     3241 agtgttttga gctgacttca tcagttctat ctgcaacctc aaaacagtgt tttgagcaac
     3301 ctgcggctag tttcctagtt tgctctttga ttttcattga gtattattaa gacaatatga
     3361 ggagaaagat gaaagaaaaa ggattttagg agggggcgca ggcagccatg ccaacggctc
     3421 ttggctatgt cagtatcggc ttggcctgtg ggactatcgg tgcgccctat gtgacacctg
     3481 ttgagatggg cttgatgagt ctctttgttt atgctgggag tgcccagttt gccatgttgg
     3541 cactgattgt ggttcaagct cctgtggcag ctattgctat gacggttttt ctaatcaact
     3601 tgcgtctctt tttgttgagt ttacacgcat cgacttattt ccgtcatacc agtctttggt
     3661 acaatatcgg tatgtctagt atcttgacag atgagaccta tggcgttttg atgggtgaat
     3721 tggcccatac agacaaggta aatcctatgt ggatgcacgg aaacaatctt aacagctatg
     3781 tggcttggtt tgtggggaca gtagtcggaa cggctctggg tggcctgcta ccaaatccag
     3841 aaatctttgg cttggatttt gccctggttg ggatgtttat tggtattttt gcttcgcaat
     3901 ttcagattat gcaaagacgg attcctgtcc gcaatctgct cattatccta gcagttgttg
     3961 cggtgtcctt ctttttgctc ttgacagtgg tgtctcagtc actagctgtt ctgtttgcga
     4021 cgctacttgg ttgtagcatg ggggtggttt tagatggtca gtaagtatct tttattagca
     4081 gttattttct ctggcttggt gacttggatt ctccgtatga ttcccttcat cttagtcaag
     4141 tataagggct tgcctgcaat cgttgagcgt tttttgaagt tcttgcccgt ttccattatc
     4201 tttgccttga ttctttcaag cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa
     4261 tggctagact tcttagccgt ctttccaaca gcttgggtag cctttcgcta ccgcaatcta
     4321 gtcggaacag ttctctttgg agtggtcttg attgccatct tgcgtttggt cttttaaatt
     4381 acccataaaa aaaacttatc acagagatag atatcatata atggcgtaaa tgctcttttt
     4441 ctgttaagat tataaggtat tctattttgg aggaaatgac atgaaaaaaa tcgttaaata
     4501 ctcatctctt gctgccctag gacttgttgc tgcaggtgtg cttgcggctt gctcaggggg
     4561 tgctaagaaa gaaggagaag cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc
     4621 accaaggcca tttatctatg aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt
     4681 tcgcgctatc tttaaagatt ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc
     4741 aggtgtcttt gctggtcttg acgctgaccg ttacaatatg gctgtcaaca atcttagcta
     4801 cactaaagaa cgtgcggaga aatacctcta tgccgcacca attgcccaaa atcctaatgt
     4861 ccttgtcgtg aagaaagatg actctagtat caagtctctc gatgatatcg gtggaaaatc
     4921 gacggaagtc gttcaagcca ctacatcagc taagcagtta gaagcataca acgctgaaca
     4981 cacggacaac ccaactatcc ttaactatac taaggcagat ttccaacaaa tcatggtacg
     5041 tttgagcgat ggacaatttg actataagat ttttgataaa atcggtgttg aaacagttat
     5101 caagaaccaa ggtttggaca acttgaaagt tatcgaactt ccaagcgacc aacaaccgta
     5161 cgtttaccca cttcttgctc aaggtcaaga tgagttgaaa tcatttgtag acaaacgcat
     5221 taaagaactc tacaaagacg gaacgcttga aaaattgtct aaacaattct tcggagatac
     5281 ttatctacca gcagaggctg atattaaata atttcttgaa atcatccatt ctgagtaaga
     5341 tgggtgattt ctcagttttt agggatatag ttttaaacta tatatctgat tatctaataa
     5401 caatttgtga aatcaaacaa attgtgagat actagtacgg tattattttt aaggagaaag
     5461 aatcatgaaa atcaaaaaat ggcttggcct agtagccctt gctacagtcg caggtttggc
     5521 tcttgcagct tgcggaaact cagaaaagaa agcagacaat gcaacaacta tcaaaatcgc
     5581 aactgttaac cgtagcggtt ctgaagaaaa acgttgggac aaaatccaag aattggttaa
     5641 aaaagacgga attaccttgg aatttacaga gttcacagac tactcacaac caaacaaagc
     5701 aactgctgat ggcgaagtag atttgaacgc tttccaacac tataacttct tgaacaactg
     5761 gaacaaagaa aacggaaaag accttgtagc gattgcagat acttacatct ctccaatccg
     5821 cctttactca ggtttgaatg gaagtgccaa caagtacact aaagtagaag acatcccagc
     5881 aaacggagaa atcgctgtac cgaatgacgc tacaaacgaa agccgtgcgc tttacttgct
     5941 tcaatcagct ggcttgatta aattggatgt ttctggaact gctcttgcaa cagttgccaa
     6001 catcaaagaa aatccaaaga acttgaaaat cactgaattg gacgctagcc aaacagctcg
     6061 ttcattgtca tcagttgacg ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt
     6121 ggactacaag aaagcacttt tcaaagaaca agctgatgaa aactcaaaac aatggtacaa
     6181 catcattgtt gcgaaaaaag attgggaaac atcacctaag gctgatgcta taaagaaagt
     6241 aatcgcagct taccacacag atgacgtgaa aaaagttatc gaagaatcat cagacggttt
     6301 ggatcaacca gtttggtaat aagaaacagg gaggtgggag agagaattcc acctcttgct
     6361 tttgtataga gcacggagtg taaagaagac tattcgttca tagaaaggta gagagaatat
     6421 ggtttttcct agcgaacaag aacagattga aaaatttgaa aaggatcatg tagcccagca
     6481 ttattttgag gttttgcgta ccttgatttc taagaaatca gtctttgccc agcaggttgg
     6541 actcaaggaa gtcgcaaatt atctgggtga gattttcaag cgtgttggag ctgaagtgga
     6601 gattgatgag agctatacag cgccctttgt catggcacat ttcaagagtt cgcgtccaga
     6661 tgccaagacc ttgattttct ataaccacta tgacactgtg ccagcggatg gggatcaggt
     6721 ctggacagag gatcctttta cgctttcggt ccgcaatggc ttcatgtatg ggcgtggagt
     6781 tgatgacgac aagggtcata tcacagctcg cttgagtgct ttgagaaaat atatgcagca
     6841 ccatgatgat ttacctgtca atatcagctt tatcatggag ggagcggagg aatcggcttc
     6901 aacagaccta gataagtatt tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt
     6961 ggtctgggaa caagggacca aaaatgcctt ggaacagctg gaaatttctg gtggcaataa
     7021 ggggattgtg acctttgatg ccaaggtaaa aagcgctgat gtggatatcc actcgagtta
     7081 tggtggtgtt gtggaatcag ctccttggta tctcctccaa gccttacagt ctcttcgtgc
     7141 tgcggatggc cgtatcttgg ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg
     7201 agaaatggcc ttgctagaaa cttatggtca acgaaaccca gaggaagtta gtcggattta
     7261 tggattggag ttgcctctct tacaggagga gcggatggcc tttctaaaac gtttcttttt
     7321 cgagccagcg cttaatatcg aaggaatcca gtctggttat caaggtcagg gtgttaagac
     7381 tattttacct gcagaagcca gtgccaagct agaggttcgt ctggttccgg gcctagaacc
     7441 gcatgatgtt ctggaaaaaa ttcggaaaca gctagacaaa aatggctttg ataaggtaga
     7501 attatactat accttgggag agatgagcta tcgaagcgat atgagcgcac cagccattct
     7561 caatgtgatc gagttggcca agaaattcta tccacagggc gtttcagtct tgccgacgac
     7621 agcggggaca ggacctatgc atacggtctt tgatgcccta gaggtaccaa tggttgcatt
     7681 cggtctagga aatgccaata gccgagacca cggtggagat gaaaatgtgc gaatcgctga
     7741 ttattacacc catatcgaat tagtagagga gctgattaga agctatgagt agagatatta
     7801 tcaagttaga tcagatcgat gtgacttttc accaaaagaa gagaaccatc acagcggtta
     7861 aggatgtgac cattcacatc caagaagggg atatctacgg aatcgttgga tattctggag
     7921 cagggaaatc aacccttgta cgggtgatta atctcttgca aaaaccatct gcagggaaaa
     7981 ttaccattga cgacgatgtg atttttgatg gtaaggtcac cttgacggca gagcagttgc
     8041 gtcgtaaacg tcaagatatc gggatgattt tccagcattt taacctgatg agccaaaaga
     8101 cagcagagga gaatgtagcc tttgccctta aacactctgg acttagcaag gaagaaaaga
     8161 aggctaaagt agctaagttg ttggacttgg ttggtttggc agatcgtgct gaaaactacc
     8221 cttcacaact atctggaggg caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg
     8281 atccaaaaat cttgatttca gacgagtcaa cttctgccct tgaccctaag acaaccaagc
     8341 agattttggc cttgttgcaa gatttgaacc aaaaattagg cttgactgtt gtcttgatta
     8401 cgcatgaaat gcagattgtc aaagacattg ccaaccgtgt tgcagttatg caggatgggc
     8461 atttgattga agagggtagt gtgcttgaaa tcttctcaga ccctaaacaa cctttgactc
     8521 aagactttat ctcaacagct acaggtattg acgaagccat ggtcaaaatc gagaagcaag
     8581 aaatcgtgga acacttgtct gaaaacagtc tcttggtgca actcaagtac gctggagctt
     8641 caacagacga gccacttttg aatgaattgt acaagcatta ccaagtaatg gctaatattc
     8701 tctatgggaa tatcgaaatt ctcgatggta ctcctgttgg agaattggtg gtggtcttgt
     8761 caggtgaaaa agcagcgttg gcaggtgccc aagaagccat tcgtcaagca ggtgtacaac
     8821 taaaagtatt gaagggagta cagtaagatg gaatcattga ttcaaaccta tttaccaaat
     8881 gtctataaaa tgggttgggc tggtcaggca ggctggggaa cggctatcta cttaactctt
     8941 tatatgacag ttctttcctt tatcatcgga ggcttcttgg ggctagtggc aggtcttttc
     9001 cttgtcttga cagcgccagg tggtgtcttg gagaataaag tcgtattctg gattttagac
     9061 aaaattacct caatttttcg tgcggttccc tttatcatcc tcttggcaat cttgtcacca
     9121 ctttctcact tgattgttaa aacaagtatc gggccaaatg cagcccttgt cccactttct
     9181 tttgcagtct ttgccttctt tgcccgtcag gtgcaggttg tcttggctga attggatggc
     9241 ggtgtcattg aggcggctca agcgagcgga gcgactttct gggacatcgt gggtgtttac
     9301 ctatcagaag gtcttccaga tttgatccgt gtgacgactg tgaccttgat ttcccttgtt
     9361 ggggaaacag ctatggccgg tgcggttgga gctggtggta tcggtaacgt agccatcgct
     9421 tatggattta accgctacaa tcacgatgtg accatcttgg caaccattat tatcattttg
     9481 attatctttg caatccaatt cttgggagat ttcttgacta agaaattgag ccataaata
//
LOCUS       DAVKZB010000069         9435 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKZB010000069 DAVKZB010000000
VERSION     DAVKZB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.069"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            49..627
                     /locus_tag="VXA80_001443"
                     /pseudo
     CDS             49..627
                     /locus_tag="VXA80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(916..1386)
                     /locus_tag="VXA80_001444"
     CDS             complement(916..1386)
                     /locus_tag="VXA80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV0353925.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(1352..2158)
                     /locus_tag="VXA80_001445"
     CDS             complement(1352..2158)
                     /locus_tag="VXA80_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0353926.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(2364..3005)
                     /locus_tag="VXA80_001446"
     CDS             complement(2364..3005)
                     /locus_tag="VXA80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353927.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(3002..5110)
                     /locus_tag="VXA80_001447"
     CDS             complement(3002..5110)
                     /locus_tag="VXA80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0353928.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(5165..5458)
                     /locus_tag="VXA80_001448"
     CDS             complement(5165..5458)
                     /locus_tag="VXA80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0353929.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            5865..6185
                     /locus_tag="VXA80_001449"
     CDS             5865..6185
                     /locus_tag="VXA80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0353930.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDDSKLIQKGVSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(6237..6824)
                     /locus_tag="VXA80_001450"
     CDS             complement(6237..6824)
                     /locus_tag="VXA80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0353931.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            7066..7737
                     /gene="sdaAB"
                     /locus_tag="VXA80_001451"
     CDS             7066..7737
                     /gene="sdaAB"
                     /locus_tag="VXA80_001451"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV0353932.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNVHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7746..8618
                     /gene="sdaAA"
                     /locus_tag="VXA80_001452"
     CDS             7746..8618
                     /gene="sdaAA"
                     /locus_tag="VXA80_001452"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV0353933.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            8640..9272
                     /locus_tag="VXA80_001453"
     CDS             8640..9272
                     /locus_tag="VXA80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0353934.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
BASE COUNT         2868 a         2084 c         1645 g         2838 t
ORIGIN      
        1 ttctaggctc aggctgaaac agtctcccag actgtttcac tcccgaatgc taaaatcgtt
       61 cttgatcgct ttcacattgt gcaacatctt agccgtgcta tgagtcgtgt gcgtgttcaa
      121 atcatgaatc agtttcatcg aaaatcccat gaatacaagg ctatcaagcg ttactggaaa
      181 ctcatccaac aggatagtcg taaactcagc gataaacgtt tttatcgccc tacttttcgc
      241 atgcacttaa caaataaaga aattcttgac aagattttaa gctattcaga agacttgaaa
      301 caccactatc agatctatca actcttactt tttcactttc agaacaaaga ccctgagaaa
      361 tttttcggac tcattgagga caatctgaag caggttcatc ctatttttca gactgtcttt
      421 aaaaccttcc tcaaagataa agaaaagatt atcaacgccc ttcaactaca ctattctaat
      481 gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc ctttggtttt
      541 cgaaactttg aaaacttcaa aaaacggatt gttatcgctt tgaacatcaa aaaagaaagg
      601 acgaaatttg tcctttctcg agcttagctg acttcaaccc actacagttg acaaagagcc
      661 ttatttgttc gttagttctt aataccagcc attgttaaac cagaagtttt tagctctgcc
      721 aagccacata gtgtgtttac tactatgtgt ccatgtcacc atgaatcgcc gaacgattta
      781 acgagaaagt atgactttta cgtttatccc aactcaatta tgacattttt ttcaaaagtc
      841 aatatatctc actttttcaa cgacaagaaa gaggctacca atcgaccaac ctcttattct
      901 gaacccatca ctccatcact ttttagcttc attcgctttc ttagcgactg caatctggta
      961 ttcgacttgg tcattcccct taccggtaca accatgagca attgtagtcg ctcctatctg
     1021 atgcgctatt tcaaccaatt ttttagaaat cagagggcgg ctcaaggcag ataccaaggg
     1081 atacttttgt tcataatagg catgtgactg atgagccact agcacataat ctgtagcaaa
     1141 ttcgtcctta acatcaatga cataagattc tactgcccca accttaagag ccttatcatg
     1201 gatgaaatct aagtctttcc cttcacccac atccatacaa actgaaacaa catcatagtc
     1261 tttctttaac caggtaatag caactgatgt gtccaaaccg ccagaatagg ccaaaattac
     1321 tttttcttta ctcatgttct tttccttctt tctatttttc aatggatgct ttaaaggcat
     1381 cctcaatgag tttgtctaat tccccagact ccttcaactt ttgaatggtt ttatcgactg
     1441 cctctttcaa ttccttgcta tcttttttca tggctaccgc ataggaatca tcttgctctt
     1501 tttcaaaatt gaggtctgcg attgctaaat caggattatt ttccacaaat cccttggcaa
     1561 cgggttcttc aaaaataacg gcatccactt gccctgattt taaatctgtg attaaattcc
     1621 catttttagg cagagatacg agggaagaat tttgtagcaa atctttcgcc atcgtctctt
     1681 gaatcgaacc tttctgcgct ccgacctttt tctgcgccaa atcgttgaca gactgataag
     1741 tggctaaatc agattttttg acaatgagtt tattttttgc agtatagtag ggagttgaaa
     1801 aatcaaacac cttgctccgt tcatctgtct tagaaacacc tgatatggca aggtcagctt
     1861 ttcctgattg aacgctagcc agtacattgt caaaactcat aggagatagt tccaattcga
     1921 cacctagttc tgttgcgata gccttggcta attcgatatc tgaacccaca atctgatttt
     1981 tcccatcaac cactttttga tattcaaatg gagcaaaatc tggattgagg gctacaacca
     2041 attttccttt ggacttgatg gcttcaattc cagccgattg attgttacta caagcaaata
     2101 gtagggggag catcactaaa ccaaacatcg tcatcaacac cttcttcatc ttattcataa
     2161 caaagcctcc tttatgttta ttctttttta ttgtataaat aatactccta tactcatcct
     2221 ttgtcaagcc taaattgtaa ttttttcaat ttgaaacaaa aaaaacctag aaatactaca
     2281 taaaatgtca ttcctaggcg tatttatgct atttttctct agaaaacatg agtattcagc
     2341 cagtcaaaag gagctgaatg aactcatttt ccctcgccca attcaatgac acgatgacat
     2401 tgttgggcta cataagcatc gtgggtcaca ataatgactg ttttcccttc tcgattcatc
     2461 tctaagagaa acttcaagac caaatctcta ttttcaggat ccagcgaacc tgtaggttca
     2521 tcggctaaaa tcagctggct gggttttaag atggctctag caactgcaat tcgttgttgt
     2581 tcgcccccag acaactcgga gaccctttga tgcaaagtag ctgacaaacc tactctctct
     2641 aaaatctctt ccaccttttt gagcttgtct ttcttaggca atttcacata tttcagcgcc
     2701 agcatgagat tgtactcgac cgtttcatca tcaatcaggg caaaattttg aaacagataa
     2761 gagatatgtt cacggattat tgtttgcgac ttagcagaat taaccgctag atttgtctga
     2821 ccaaaaatct cataccgtcc gctataatca ccatctatca aacccaataa atttaacaag
     2881 gtcgacttcc cactaccact cttaccaaca atagctacca aatccccctg atcaatcctg
     2941 agagataagt tatccaaaat cacttttccc ccaatggttt tggtaatatt tttcaactca
     3001 atcataagat gccccctttc aataactcta ctagacttct tttctccatc ctagaagcta
     3061 agcctagcac aaatagtata tccagacatg taaaacctgc aaacagtaga agtggtaaga
     3121 acgcatgggc aaagaaaatc aagactagaa gagggaaact atagcccagc aagagcagaa
     3181 cgaggagagg acggtagcga tcgaccagtt tccaccccat aaacttcttg gtaatgatat
     3241 ccctgcgctt caataagaaa gttgttacta gtaagaagta ggaaatcatc atgctaagga
     3301 gaccaaacaa agcaaagagt aggttaaaat tccgaacagc atctcgataa gaatccactt
     3361 tctcttgttg aatggcttga atagatgaaa attttaaata atttccatct gacaatttct
     3421 caactaactc tgtaatctct ttttgatgtt gaaccgtatt ttcaatttta atcggattat
     3481 ttaagccagt tgttgacagg gaggctttct catcccacat catatcagaa tcattgacca
     3541 agctaataat tggattggag agattttcct ttcgcttatc actatatggg aaaaatgacc
     3601 aatctccttc ataataggca atctcgacat ccatctcctc tatcgttcgt ttttgctgct
     3661 cttcatactt catcgaatga aaggcaatta acttccccaa gagctgattt ttatcttctt
     3721 cacctttcgt acttgctggc atcaaaataa cttttttaat accggtatct ggtagcttga
     3781 atcccttgct ctttagaaaa ttgcgattgg catagtaaac atccaccgta tctgttaact
     3841 gatattgctg aatctgttct gattggacaa aattttttac aggaagactg ctactctgca
     3901 catagcccgc ctgcgttttt tctaccaaat cctgataaaa tcgatagaaa taatctgtag
     3961 atttccctga ccctgctagc tcttcttgcc acagattatc attgagtttg aaggtttcta
     4021 aggtcaggta attaccttga cttacccact gttgctgata agcaagttct ttgttttctt
     4081 gttctaaact tctgcccacc ccaatcagta aggccgtcag taaaatagtt gtccctattt
     4141 tcatcacata attgaagata agaccaaatt tgaaagatga aaaacctttc agcagagagc
     4201 tgattgtcat tttttggatt aagaggtaag tcaaccaact gataaagaga taaagctgca
     4261 acagcaaaaa atgagacaac cacagcatag gaaacaaatc ttttggctta taatcaagca
     4321 agaaaaacac gcctagattg atcacaagag ccccacctag gaggaggtaa aggttgcctt
     4381 ttacaacatc agctaaaaca gccctatctt gaaaaccaag taatttttgt accccaactc
     4441 ttttcatctc catcatcggt tgatacactg tcactaacac aagaagcaaa atagccaaga
     4501 caaaaacaat ggcagataaa agcaaatctc gatttatgac ttccactgca cttttgtagg
     4561 tcggctctag caaggtagcc tggtctatct tgaaaaaatc gctccatttc tgtacaatcc
     4621 tatccttgtc catctcttgt gtagaagtta tcgtatagcg accatttaaa ctacgagatg
     4681 tatccttgat ataggtttga aaagtcataa gctgaatagg tttggctttt agaaaggtcg
     4741 gaatcgtacc aagtttattg gaaatttctt tattactata gactccttca ccatctgtgg
     4801 taaaatcaag agaagaaatc ccaaactctt ggtaggggaa ggtatcttta tcaaaaacac
     4861 cagacttgac cacctcatca ccactgtctg ttttgatgat ggagacttta tactcctttg
     4921 atacatcctc aaaaaatcga agaacagacg ctgcaggttc gttaatatct ttcaaataca
     4981 aatccaaaga atctacagtc tttcccattt cttgaatccg aaccacttct cgtgtatagt
     5041 ttacattaaa aacaaaaaaa gtggtacaca gaagcaggag taacatacaa agatgactga
     5101 tttttttcat agagatacct cccgtgaaga aactccctct ccaaaagggg agggagaatt
     5161 ttaattaaaa tccatagaaa tattccattc ccgttggtgg gaatttgaag attgaagccc
     5221 gagcccatgc ctcagcttct gcacgatcct tagagaattt atttccatct ttaacagttg
     5281 ctgtatgaga gcgagtagaa tgtaaataat cagaataacc aaaatttcct gaccatccta
     5341 ctccatagtt ccattgacca ccatcaaccc atactgccaa aacacttgta gaagcaagta
     5401 tactacatac cattaaagat gcaacaccaa ttttctcatg atttatttct cctattatcg
     5461 agagctattc cgtagaaaca gctatagtaa tttgtcaaca agtatcgtag tccattggac
     5521 tcaccttcct tctatctcat ttcattttta aacaacgaac ttactcttaa ctagaagaat
     5581 agttctcttc tgtatcaggt cttttaacaa ccagagtagt attaatcaca ctctaactat
     5641 tactcaagaa tcagcccaat aaacaatatg gatagcgctt tcaaattttt aaactcttat
     5701 cccatccttt tatctatata ataagtgaaa atataataac tgtcaagtaa tcgaagtgaa
     5761 ttttataaaa aaaattacaa gccaaatttg caaagtttac aataagccgc tagggcaatc
     5821 gtctatcaga atgtccgttt atttgtcaat aatccgagaa aatcttgcaa cgcttagaag
     5881 tctataaaaa ctatcaacat ttatatgact tgcgaatagc aatcctgctc aacctttcca
     5941 cactctatct atacaatcaa gataaaaaca tgtgtaagca aatctgctac actttactag
     6001 aggacgccaa gaataagaaa agctacgata ggctggctat ctgctatgtc cctattggga
     6061 tttgtacaga cgattctaaa cttatccaaa aaggggtctc ccttctggag ctaaccgagg
     6121 aaacttctat gctgtctcat ctcaaaaaag aagtagagat ctattaccaa gcgaaggaaa
     6181 gataaaaaag ccgagggatt tcctcgactc tttacatatt atttgttcgt tagttcttaa
     6241 taccagccat tgttaagcca gaagttttta gcagcagtcc atgaaccgta acgtcctgca
     6301 acgtaggcat ctgctacacg ttcttggttt tcagctgagt agtcaccgtt caggtatgaa
     6361 tctgttaatt ggtaacgtcc gatataacgt ccatttgtag ctgtatagct accacctgat
     6421 tctttttgag cgatccattc tttggcttct gcttcagatc cgcttacagt tgaaacaact
     6481 gtttcactta ctactggagt ttctgctact ggagctgaaa cagtttcatc ttgagcggct
     6541 ggtgccgcat aagtagctgg cgctggtgtt gcaacaggcg ctacagggcc atcgataacc
     6601 aactcttgac caacataaat caaatgaatg ttatcaatgt ggttgttttc tgccaatttt
     6661 tcaactgttg tgttgtgagt ttcagcgatt tctgaaagtg tatcaccttc tttaacagtg
     6721 taagttgatg attcttgagc agatacaaat gatggagcaa atactgcaaa caaggcagct
     6781 actcctgcaa gagttgcttt aatcttttta gttattgatt tcatattcga aaattctcct
     6841 tctttctata gttctatcat accctttaaa tattaccgtt tcttgacact tttatgtaga
     6901 aatattacaa aattattttt tatttcccta aataaggtgt tttttgtcat aaaagatact
     6961 atttttatgc tatttgaggt attagaaagg ttttcatcca caattaaagc caagcccctc
     7021 attctttgat ataatagaga taagaatttt tataagggga aactgatgaa atcacttcgt
     7081 tttcaatctg tctttgatat catcggacca gttatgattg gcccatctag tagccatacc
     7141 gctggtgctg ttcgtattgg gaagattgtc tcttccattt ttgatgatac tccgacagaa
     7201 gttgaattcc aactttttaa ctcatttgcc aagacctatc gtggtcacgg aacagaccta
     7261 gcccttgttg caggtatttt aggcatggat acagatgatc ctgaaattcc aaacagtctg
     7321 gagattgccc acaagcgtgg tatcaagatt gtctggacca ttcagaaaga cagcaatgcc
     7381 cctcacccca acaccactaa aattaccgtc aaaaatgtac acaagaccat cagcgtgact
     7441 ggtatttcta tcggtggagg aaatattcag gtaaccgaac tcaatggttt tgccgtctct
     7501 ctcaatatga atacaccgac tatcatcatc gttcatcaag atattccagg tatgattgcc
     7561 cttgttacag aggcgctttc ccgctatggt atcaatatcg cccagatgaa tgtcactcgt
     7621 gaaaaagctg gtgaaaaagc cattatgatt atcgaagttg acagtcgcaa ctgcgatgag
     7681 gctatcgaag aaattcgaaa aatccctcat ctccacaatg tcaatttctt taaataggag
     7741 gaagcatgtt ttattctatc aaagaattgg tcgagcaagc agatctggac tttcaaggaa
     7801 atgtcgcaga actcatgatt acaacagagt ttgaattgac cggtcgcgaa cgtgaagaag
     7861 tcttccttct catggaacgc aatctggaag ttatgaaagc ctctgtccaa cttggcctca
     7921 atgaaaataa atctcgtagt ggcctgacag gtggagatgc tgccaaattg gatcactaca
     7981 tcgaaaacgg aaaaactctg tcagattata cgattctctc ggctgcccga aatgccatcg
     8041 cagtcaatga acacaatgcc aaaatgggct tggtctgtgc cactccaacc gctggaagtg
     8101 ctggctgtct gccatccgtt ctcactgctg ctattgaaaa attagacctc agccacgagc
     8161 aacaattgga tttcctcttt gctgctggtg cctttggact agtcatcgca aacaatgcct
     8221 ccatctcagg tgctgagggt gggtgtcaag ctgaagttgg ttcagcctct gctatgagtg
     8281 ctgccgcctt gactctggct gcaggtggaa caccttatca ggccagtcaa gctattgcct
     8341 ttgtcattaa aaatatgcta ggcctcatct gtgaccctgt tgcaggtttg gtcgaagttc
     8401 cctgtgtcaa acgtaatgcc atgggagcta gctttgcttt catcgcagca gacatggcct
     8461 tggcaggtat cgaatctaaa atccctgtgg atgaagtgat cgatgccatg taccaagtag
     8521 gagcaagcat gccaactgcc tttcgtgaaa cagctgaagg tggactcgct accaccccta
     8581 ctggtcgtcg cctccaaaaa gagattttcg gagaataagc ttatcaaata ggagaaacca
     8641 tgacctctat cacagcgatt tttttcgatt tggatggaac cctcgttgat agttctatcg
     8701 ggattcacaa tgcctttacc tataccttta aagagctggg ggtgcctagc cctgatgcca
     8761 aaactattcg tggttttatg ggaccacctc tcgaaagtag ttttgcgacc tgcctgtcca
     8821 aagaccaaat ttctgaagcc atgcagatat atcgttctta ctataaggca aaaggcatct
     8881 atgaagctca actctttcct cagattatag acttgcttga ggagttatcg agcagttatc
     8941 cactctacat caccacgaca aagaatacat ctactgctca agacatggcc aaaaacttgg
     9001 aaatccatca tttctttgat ggtatttatg gttctagccc tgaagcaccc cataaggcag
     9061 atgtcattca ccaagcctta cagacacatc aactagcacc agaacaagcc atcatcatcg
     9121 gagataccaa gtttgatatg ctgggagctc gagaaacagg tattcagaaa ttggccgtca
     9181 cttggggatt tggagagcaa gcagatctac taaactatca acctgattat atcgctcaca
     9241 aacccttaga agttttggcg tattttcaat agcatagact gggtaacaac tccatttcag
     9301 aagaaaatga agtaatactc aatgaaaatc aaagagcaaa ctaggaaact agctgcgggc
     9361 tgctcaaaac actgttttga ggttgcagat agaactgacg aagtcagctc aaaacactgt
     9421 tttaaggttg tggat
//
LOCUS       DAVKZB010000070         9241 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKZB010000070 DAVKZB010000000
VERSION     DAVKZB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.070"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..1109)
                     /locus_tag="VXA80_001454"
     CDS             complement(<1..1109)
                     /locus_tag="VXA80_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0353935.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIKWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRT"
     gene            1167..2333
                     /locus_tag="VXA80_001455"
     CDS             1167..2333
                     /locus_tag="VXA80_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV0353936.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2472..2798
                     /locus_tag="VXA80_001456"
     CDS             2472..2798
                     /locus_tag="VXA80_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0353937.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            2785..3378
                     /locus_tag="VXA80_001457"
     CDS             2785..3378
                     /locus_tag="VXA80_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV0353938.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3371..4282
                     /locus_tag="VXA80_001458"
     CDS             3371..4282
                     /locus_tag="VXA80_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV0353939.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4345..5409
                     /locus_tag="VXA80_001459"
     CDS             4345..5409
                     /locus_tag="VXA80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353940.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5481..6341)
                     /locus_tag="VXA80_001460"
     CDS             complement(5481..6341)
                     /locus_tag="VXA80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV0353941.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFAIVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(6463..7449)
                     /locus_tag="VXA80_001461"
     CDS             complement(6463..7449)
                     /locus_tag="VXA80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV0353942.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7721..9196)
                     /locus_tag="VXA80_001462"
     CDS             complement(7721..9196)
                     /locus_tag="VXA80_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV0353943.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
BASE COUNT         2692 a         2024 c         1655 g         2870 t
ORIGIN      
        1 gttctcttgt actccttctt ccatttaaat cctctaatta aacctacaat cgtcccaaag
       61 ccataagcaa agtgaatgga aaataaaaga aagggcatca caattagaaa tccattttta
      121 tgttttaata aagtcagcaa agtgagtagt gacaaaagta gaaaataggc acctaatagt
      181 aaagttatga atacgaatgt gatcggtaac aatgctagac taaacacaag actcaaaaca
      241 aataaacaag gaacatagtg aaataatgat aaacacttaa actgaacatg acttgtcaag
      301 ccaatccaca aaccatttaa atacttttga tgcagcattt tcttgaatgt tggtcgaata
      361 tactgataag atagaatact tgggctatag cggattttat aaccatattc tcgaattcta
      421 taatgaatat cattatcttc agttcggcca agttgctcat ttactaaacc aaccttctgg
      481 aaaacctctc gtttatacat tccatgaaaa atagaagaaa catatctatc ctcagaacta
      541 tttcgataat tggcaatgct actgccaaac atattttcct caacaagatg caaggtctct
      601 gcccattttc cttttccttc gacaatcgtc ggtctaggcc ccccacagac aaattcacct
      661 tgttgaataa tagccacatt gttcattaca aaactctcag taacttttga atgagcatca
      721 atttttaaaa taaggtcccc tacagaatgt ttaactccca ggttaaaacc actagcttga
      781 tttttcttag gattgttata caatctaatt gagttaaact ctgtatcttc ctttataaat
      841 tgctgaatga tagctgtggt cccatctgtg gacatagcat ttataaatag aatttcaata
      901 ttctctttag gataagtttg attttttaag tcttcaatta gaccaggcag atatttttct
      961 tcattgtatg cactgattac aatggataca agaagacttt caacattttg agtcactact
     1021 actctcctga atttttcctt ccaccatttt atcataattt tcaagacttt cccattttta
     1081 tcttgaaagc cagaaacctt acttgacatt atagtgaaaa agccttaaaa taagctgtta
     1141 ttaaaatcag ctagaagagg tattatatga aaaagcaatc actctttttt gttccaggta
     1201 ttatcctgat tggtgtttcc ttgcgaactc cttttactgt tttacccatt attttgggaa
     1261 atatttcgca aggtctggag gtagaagtta gttcgcttgg tgtcttgacc agcctgcctc
     1321 tccttatgtt taccctcttt tcaccatttt ctacccaact ggctcagaaa atcggcttgg
     1381 agcatctctt cacctacagc ctcttcttct tgaccatcgg ctcacttatt cgactaatca
     1441 atctgcccct actctatcta ggaaccttga tggttggggc aagtgtcgca gtcatcaatg
     1501 tcctgcttcc tagtcttatc caagccaatc aaccaaagaa aattggtttt ctgaccacct
     1561 tatatgtaac gtctatgggg attgcaacgg ctctggcttc ctatctagct gtgcccatta
     1621 cacaagccag ttcttggaaa ggacttatcc ttctcctcac gttactctgt ctagcaactt
     1681 ttttggtctg gctcccaaat caccgctata atcatagact agctccacaa accaaacaaa
     1741 aaagtcaaat aaaggtcatg cgtaataaac aggtttgggc aattattatc ttttcaggtt
     1801 ttcaatcctt gatcttttac accgccatga cctggttacc taccatgtct atccatgcag
     1861 gtctatccag tcacgaagct ggcttgctga cttctatctt atctctgatt agcattcctt
     1921 tttcaatgac catcccaagc ctgacaacca gtttatctac tcgcaaccgt cagctcatgc
     1981 tcactctggt ttcactagct ggtgtggtcg gcatttccat gctctttttc ccaatcaata
     2041 atttcattta ctggcttgcc atccatctcc tcatcggaac cgcaaccagc gccctcttcc
     2101 cttatctcat ggtcaacttt tcactcaaga caagcgcccc tgaaaagaca gcccaattgt
     2161 ccggcctatc tcaaacagga ggctatatcc tagcagcctt tgggccaacc ctctttggtt
     2221 acagttttga cctgttccac tcttgggtac catctgtagc tgccctcttg ctcatcgata
     2281 tcctgatgac tgtggccctc tttacagtgg acagagcgga taagatcctt taaacttcta
     2341 gttttagcta gaagtttttt atatataaaa attttacaca tttctcttga cagggctttc
     2401 ttttagatgt acaatgtatg tgtagaaaaa ttatatataa aaatcttaca cattagaaaa
     2461 ggaggtttcc catgtacttt ccaacatcct ctgccttgat tgaatttctc atcttggccg
     2521 tactggagca gggtgattct tatggttatg agattagcca aaccattaag ctcatcgcta
     2581 atatcaaaga atccacactc tatcccattc tcaaaaaatt ggaaggcaat agctttctga
     2641 caacctattc tagagagttc caaggtcgca tgcgcaaata ctactccttg acaaacggtg
     2701 gtatagagca gctcttgacc ctaaaagatg aatggacact ctatacagac accatcaatg
     2761 gcatcataga agggagtatc cgccatgaca agaactgaat acctgactca gctagaactc
     2821 tatctcaaga aactacctga agctgaccgt atcgaagcca tggactattt cagagagctc
     2881 tttgacgatg ctggagtcga aggagaagaa gaactcatcg ctagtttggg aactcccaaa
     2941 gaagcggccc acgaagttct atccaatctt ctcgataaaa aaatcaatga agcacccgct
     3001 caaaaaaata accgacaaat tttacatatc gccttgttag ccctccttgc agcacctatc
     3061 ggcattcctc tgggaatcgc catcctcgtg accctgttcg caatccttgt agccgctttg
     3121 actgtcattc tggctttctt tgcagtttcc atactgggta tcatcggcgg attcctattt
     3181 ttagttgaaa gtttcactgt cctcgcccaa gccaaatcag cctttatctt gatttttggt
     3241 tctggtttac tggctatcgg tgcttcttcg ctagttttac ttggcatttc ctatgtaact
     3301 cgcttcttcg gtctactcat tgttcgtctg gtacaatttg ttcttaaaaa aggaaagaga
     3361 ggtaaccagc atgcgtaaat ggacaaaagg atttctcatc tttggtgtgg tgactaccgt
     3421 tatcggcttt atcctgcttt ttgtaggtat ccaatctgac gggattaaga gcctactttc
     3481 catgtccaaa gaacctgtct atgatagccg tacggaaaag ctaacctttg gcaaggaagt
     3541 cgaaaaccta gaaattactc tccaccaaca cacgctcacc atcacagact ctttcgatga
     3601 tcaaatccac atttcttacc atccatctct ttctgctcac catgatctta tcaccaatca
     3661 gaacgataga actctgagtc tcactgataa gaaactgtct gaaactccgt ttctctcttc
     3721 tggaattggt gggattcttc atatcgcaag tagctactct agtcgttttg aagaagttat
     3781 tctccgacta ccaaaaggga gaactctaaa agggatcaac atctcagcca atcgcggaca
     3841 aaccaccatc ataaatgcta gccttgaaaa tgcgaccctc aatacaaaca gctatatcct
     3901 ccgaattgaa ggaagtcgta tcaaaaacag taaactcaca acacccaata tcgttaatat
     3961 ctttgataca gttcttacag atagtcagct agagtcaaca gagaatcact tccacgctga
     4021 aaatatccaa gtccatggta aggttgaact gactgccaaa gattatctca gaatcatcct
     4081 agaccagaaa gaaagccaac gaattaactg ggacatctca agcaactatg gttctatctt
     4141 ccaattcaca agagaaaagc ctgaatcaag aggtacggaa ttaagcaacc cttacaaaac
     4201 tgaaaaaacc gatgtcaagg atcaactcat tgcgagatct gatgataata ttgatctaat
     4261 atccacacca agcagacgtt gacaaaaacg cttacatctg ctactatgga agcatattta
     4321 tctaactaaa aaggaggttc taccatgaaa caagaatggt ttgaaagtaa tgattttgta
     4381 aaaacaacaa gcaagaacaa gcctgaagag caagctcaag aggttgcaga caaggctgaa
     4441 gaaacgatag ccgatctcga tacaccaatt gaaaaaaata ctcaagtaga ggaggaagtc
     4501 tctcaagctg aagtcgaatt ggaaagccag caagaagaga aaattgaaac tcctgaagtc
     4561 ggtgaagcga gaacagaaat agaagaaaag aaggcatcta attctactga agaagagcca
     4621 gacctttcta aagaaacaga aaaagtcact atagctgaag agagccaaga agctcttcct
     4681 cagcaaaaag caaccacgaa agagccactt cttatcagta aatctttaga aagtccttat
     4741 atccccgacc aagctccaaa atctagggat aaatggaaag agcaagtgct tgatttttgg
     4801 tcttggctag tggaagcgat caaatctcct acaagtaagt tggaaacaag tatcacacac
     4861 agttacacag cctttctctt gctcattctg ttttctgcat cttccttttt ctttagtatc
     4921 tatcacatca aacatgctta ctatggacat atagcaagca ttaacagtcg cttccctgag
     4981 cagctagctc ctttaactct tttttctatc atctctatcc tagtagcgac aacactcttc
     5041 ttcttttcat tcctcttggg tagtttcgtt gtgagacgat ttatccacca ggaaaaggac
     5101 tggacgctag acaaggttct ccaacaatat agtcaactct tggcaattcc aatcttcctc
     5161 actgctattg ctagtttctt tgctttcttt gatagcctac gatttacagc cctcttgtgt
     5221 gtgattagca ttggaatcat tctgcttgcc agtctccata tcattacaag accaagtcaa
     5281 gcaagtgaaa ccgactcctt ctatcaatta ttcttgtctg tccttgtgaa cggagttatt
     5341 atcctcctct tctttgtagc tgaagtggca ctgattggag attatcttcg tatcttggcc
     5401 tttctttaaa attcaatcct gtcatccttg gcaggatttt ttctatgtca aaaaagcaag
     5461 tcgattgacc tgcttctgct ttacttttct tgtgctaatg ttttaaaatc ttgtcctagg
     5521 atgggttgta tttctatttg attggcgtct agttggtggt aaaaagatgg gggtaatacc
     5581 tctaagaatt ttgcatagtc caagcgagct atggattcat agtcttctgg tttagagccg
     5641 tctcctgtga tttgaaccaa gtaaatctcc cactggactt ccttatccac caattgctca
     5701 atataggctg caggaacaaa cggttctctc ggtaaaactg tcttgactcc ttgagctaaa
     5761 tggtaaacgc catgcacttt tccttctcca tcctgataga aggaaactat cgcaaagtag
     5821 gcatctgtct cttgaaccgc acgcacacgc gcttcaaact gagccaagcc atctgcctct
     5881 aaaaagcttt tcaagttctc acgactaaag aaaataggat taaaaattcc ttggcaaatc
     5941 gtaaaacgag ctacagggtc tgctagatag gcttgaatac ttgcttggaa ataagcttca
     6001 aaatcaaaac catctagccc ttcaatatca gtccctgtat aagcaagcaa agcatctaaa
     6061 aatgacttga taatctgcca gtctgtcttt gttagtaggt caggaagatc gacacggtaa
     6121 gccttttgac catcagcata actgacctta aagagaaatt gtgattgccc cactatcgca
     6181 cactcaatat actcgagacg attaagaggc tgacgaagat agactacatc atagctatgt
     6241 gactccaaac cgtctagcaa ggccaagatc gacttggcat tcaaaatctc ttgttctcct
     6301 aaaatgctct gtttatttgg aataaaaaat gtttttgcca taactggact cctttgaaat
     6361 cgtttacata cagtatacct tattttcctg aaagatacaa aaacaaactt tagatttttg
     6421 agtacaaagc atagaaaaac agcccctgag gactgtttga tcttatacag tagctgcctg
     6481 atccaagctt tcaccgatag cggctaggcg ctcaatcact tcagcttgtg tcaattcatt
     6541 ttttgaaaca tagcggttac gtgggtgaac acggcactcg tgtgagcatc cacgaaggta
     6601 cttgtcttca ttttcttctg atgtcaagat acgacggtta cagaagggat ttccacagtt
     6661 aacataacgt tcacatggtg ttccatcaaa ccagtctttc cctacgatgg ttgggttgac
     6721 atggttgaca tcaacggcaa tacgctcgtc aaagacgtac attttcccat cccaaagctc
     6781 accttgaact tctgggtctt taccgtaagt tgcgattcct ccgtgcaatt ggccgacatc
     6841 tttgtagcct tcacggacca tccagcctga gaatttctca cagcgaacgc cacctgtaca
     6901 gtaaaccacg acacgcttgt ccatgaattt ttccttgtta tcacggaccc attgtggtaa
     6961 ctcacggaag ttgcgaatat ctgggcgaat agccccacgg aaatgtccta ggtcgtactc
     7021 ataatcgtta cgtgtgtcaa ggacaacggt atctttatca agaagcgctt ctttgaactc
     7081 ttttggagac aagtaagcac ctgttgtttc aagtgggttg atgtcattgt caaagtcgtt
     7141 gtcttccaaa ccaaggtgga caatttcttt cttgtagcga acaaacatct tcttgaaggc
     7201 ttgttcattt tcttcgtcaa tcttgaacca gagatcttcc atacctggga ggctgtgaac
     7261 gtagtccatg tatttttgag ttgtttcata gtcacctgaa actgttccgt taattccctc
     7321 gtcagcgact aggatacggc ctttaaggcc gattgattta cagaaagcca agtggtctgc
     7381 agcaaattgc tctgcatttt caattggagt ataaaggtag taaagtaaga cacgaatatc
     7441 ttttgccata agatttgttc tcttttctat tcttaaattt tctaaatttt ttatttatcc
     7501 atactagtat acctgcttga gaaaacgaat gcaatttatt tgactggaaa ttgtagagga
     7561 ttctcgaaat ttcctgaatg gttaaaataa ggacaagaga aaacagggat atctatatcc
     7621 ttgtgccaaa aaaaccactg ccctccccag accaacctga ggaaagcagt gattcttatt
     7681 ttaggagtta ggaatgaata cacgaaatca atttagctga ttattttttg tttttcaaga
     7741 attcatcgta ttgtttttgc atttcgttca atactttttc gtaggcacct tcagatttca
     7801 atttttccat caattctgga atcgctttat ctgggtctac agtaccagtg ttgatagctg
     7861 tatcaaattg ttgcattgtg ttagcaatag ctgagatttc agatttcaca ttgtcagtat
     7921 tgaagataaa tccaagcgct ggagattctt tagcttctgc caattctttc ttagaatttt
     7981 cgatttgttg gtctgtaacg ttttcgttga tgtaaaggat ccagttgtta ccagtgttcc
     8041 atccacccat gtgagtgttt cctttgtagc catcaagaac gcgaacacgg ttttctttac
     8101 cttcaatttt ttcccagttc ttgccttctg gaccgtaaac aagaccgttc aagagttctg
     8161 gattcgtatt caagaggttc aagatttcca ttgatttttc tttgttctta gagttgtttg
     8221 agatgacaaa gttagcaact tgtgttgttt ggtttttctt gatgaagtta gtaattggtt
     8281 tgatttggat atctttgttg gcaacacgtg aaagcaagct gttaccgtag tcagctggtc
     8341 ctactgtttc ttcacgaacg aaccaagtat cttgttgaag gtcaaaggaa gtatcgcttg
     8401 ttgcgacgtc ttttggaatg tagccagctt catagaattt gtgaagagtc ttcaagtgtt
     8461 ctttgaaacg aggcacttcg taacggttta caactttagt agtatcgcct tcaaggtcga
     8521 taacgaatgg aagaccgttt gctactgggt agtcaaagtt atcagatggg atgaaaactt
     8581 taccaatagc aaatggtact acgtctggag ctttttcttt aatttgtttc aagactggct
     8641 caagagtttc gtaagaagta acacctgaaa tatcgatacc atatttagca aggagagttc
     8701 cgttgaaggc aaagttttga gatgatgcaa cgttggctgc aactggaaca gcgtaaatct
     8761 taccatttac agtattaccc ttgatgtaag ctgggtcaag tgccttgtaa aggtctttac
     8821 cttctttttt gtacaattct gtcaagtcag cgtaagcacc tttttgagca tttacaatat
     8881 agttatctgc aaaggcaata tcatagtttt caccagatga tgtgataact gacattttct
     8941 taccatagtc accccagcca aggtattgga tatccaattt agcaccaact ttttcttcga
     9001 tgattttgtt ggcatttgct aacaattcat ccaagttatc tggtttgtca ccgatttggt
     9061 acattttgat aacaggtttg tcacctgaat cagcagcttt tttgctgtta cctgtcaaat
     9121 ttccacaagc agcaagacct gcagccaaag cgactacact agcagatgca aaagcatatt
     9181 ttttccagtt tttcatgata aaaactcctt tttttatttt taaacttata aacaatgtaa
     9241 t
//
LOCUS       DAVKZB010000071         9200 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKZB010000071 DAVKZB010000000
VERSION     DAVKZB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.071"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            408..1883
                     /locus_tag="VXA80_001463"
     CDS             408..1883
                     /locus_tag="VXA80_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV0353944.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1849..2564)
                     /locus_tag="VXA80_001464"
                     /pseudo
     CDS             complement(1849..2564)
                     /locus_tag="VXA80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2820..3680
                     /gene="speE"
                     /locus_tag="VXA80_001465"
     CDS             2820..3680
                     /gene="speE"
                     /locus_tag="VXA80_001465"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV0353945.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3677..4936
                     /locus_tag="VXA80_001466"
     CDS             3677..4936
                     /locus_tag="VXA80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV0353946.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIGSYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4936..6063
                     /gene="nspC"
                     /locus_tag="VXA80_001467"
     CDS             4936..6063
                     /gene="nspC"
                     /locus_tag="VXA80_001467"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV0353947.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6060..7145
                     /gene="aguA"
                     /locus_tag="VXA80_001468"
     CDS             6060..7145
                     /gene="aguA"
                     /locus_tag="VXA80_001468"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV0353948.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7155..8030
                     /gene="aguB"
                     /locus_tag="VXA80_001469"
     CDS             7155..8030
                     /gene="aguB"
                     /locus_tag="VXA80_001469"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV0353949.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8211..9020)
                     /locus_tag="VXA80_001470"
     CDS             complement(8211..9020)
                     /locus_tag="VXA80_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0353950.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2686 a         1781 c         2069 g         2664 t
ORIGIN      
        1 atgatatagt aataaagctt agaaaatgag atgatgtttt ctagcaaata taaacccgag
       61 taaaaaatgc ctacggacag gcagggttga atgccgaagc gtggttgaaa agccacatta
      121 ttgatagggt taaaagccta cttttataag ttgatgttag gacacttgtc ctaattcata
      181 aatttttagt gtggtgaaag cacacgtcat cttgtgaaac gatcaataaa gtacgtaata
      241 tttgctacta gagagttagg aaacatcggg aacagacata ctcaacagaa accaaaataa
      301 acacgtcaga agattgcaga gcaggtgaaa acctgctctt ttttcatgag tcaaccttta
      361 gttccttagt tttcataagg tcctaaaaat attgaaagga gtatgttttg aaagagttag
      421 atcaaaacca agccccaatt tatgaagcct tggtgaagtt acgcaagaaa aggattgttc
      481 cctttgatgt tccaggtcac aagcgtggac ggggaaatcc agaacttgtc gaactcttag
      541 gagaaaaatg tgtaggcatt gatgtcaatt cgatgaaacc cttggataat ttaggccatc
      601 ctatttcgat tattcgtgat gcagaggagc tggctgcaga tgcttttgga gctagccatg
      661 cctttctaat gattggtgga acaacttcat cggtgcagac tatgattctg gcaacctgca
      721 aggcaggaga taagattatt ctgccacgaa atgtccataa atctgctatc aatgcgttgg
      781 ttctatgtgg tgccattccc atctatatcg agatgagtgt agatcctaag attggtatcg
      841 ctttaggtct tgaaaatgac cgagtagcac aggccataaa ggaccatcca gatgctaagg
      901 ctatcctaat caacaatcct acttactacg gcatctgttc agacctaaag gggttgacag
      961 aaatggctca tgaagctggc atgatggttt tagtagatga agcccatgga gcgcatttgc
     1021 atttcactgg taaacttcca atttctgcta tggatgcagg agctgatatg gcagcagttt
     1081 ccatgcataa gtctggtggg agtttgacac aaagttcgct tcttttaatc ggggagcaga
     1141 tgaatcccga gtacgttcgt cagattataa acctgaccca gtctacatct gcctcttact
     1201 tgttgatggc tagtttggat atttcacgtc gcaacttggc ccttcgtggt aaagagtcgt
     1261 ttgagaaagt cattgagcta tctgagtatg cccgccgtga aatcaatgct atcggtggct
     1321 actatgccta ctcaaaagaa ttaatagacg gtgtttcggt ttgcgatttt gacgtaacta
     1381 agctgtcagt ttacactcag ggtattggct taacaggtat cgaggtttat gacctcttgc
     1441 gagacgaata cgacattcag atcgagtttg gtgatatcgg caatatcttg gcctatattt
     1501 ctatcggcga ccgcatccaa gacatcgagc gcttggttgg tgctctggct gatattaaga
     1561 gactctattc aagagatgga aaagatttga tagcaggaga atatattcag cccgagttag
     1621 tgctgtctcc gcaagaagcc ttctattcag caagaaaaag tttaactttg gatgattctg
     1681 ttggacaggt ctgtggagaa tttgtcatgt gctatcctcc aggtattcct atcttggctc
     1741 ctggtgaacg cattacacga gaaattgtcg actatatcca attcgccaag gaacgtggtt
     1801 gctccctcca agggacggaa gatccagagg tcaatcatat caacgttatt aagagaaaga
     1861 caaactataa gaaaagtcaa tagtttatct aaactatttc ttatttcaat ttgatgattt
     1921 ggcgatgatt ttagagcacg gcaaaaagcc cttgaattag aagcggtcaa tcgcttaatt
     1981 tctatcagct tatcaaatcc tgcctcaatc cttttctgag gattagggta gcgtgtcaag
     2041 agttggtagg tatattctga atgctttcca acgattttat ccaactcagg aaagatgata
     2101 tcaaggcaac gagtgtattg tactttccaa tcagactgtt ttttcttgag acgatgaata
     2161 tgtctagtca gtatttttag ttctacttgc cgattattgt gttgaaattg ttcacgattg
     2221 gggtcagaaa gaagtttaag agcgatgcca tgagcgtctt tcttatccgt tttagtcttg
     2281 cgaagtgata atgatttggc aaatttcttg atgagcaaag gattgtaggt gtaaacttta
     2341 tatccttgtt catgcaggaa gttcagtaga ttaaaggcat aatgtccagt atcttcaaga
     2401 gcgatgagac agtcttggtt gagctgtcga agagacagat ctaagagttc aaaaccagct
     2461 ttattatttg aaaaagtgag tggtttaaga acagtttttc cttgaacatt caaggctgta
     2521 acatcgtgtt tatttttagc gacatcaatg cccacataaa gcatgggagt acctccagat
     2581 gtagtatttc aagtctactt ggttatccac gaactttttg ccttgttacc ttagacgaga
     2641 taaaacgtct atgcgttatc aaactcatta ccaattgaaa caaaaagctg tggttagatc
     2701 ctttcggaaa tcgtcaagcg attggaggaa atgaactaat ccacagtggc ttattccaag
     2761 tataccactt gggctttggc agtagctaac tgcgctaaat ataatataag gagaaataga
     2821 tggatttatg gttttctgaa gttcatactc cagatgtcaa attgtctctg agaacagcca
     2881 agcaacttta cgctggaaaa agtgaatggc aggatatcga agtcttggat acgccagctt
     2941 ttgggaaaat actgatttta aatggccatg tcttgttctc agatgcggat gatttcgtct
     3001 acaatgaaat gaccgttcac gttcccatgg ctgtccaccc aaatccaaag aaagtattgg
     3061 ttattggggg tggtgacggc ggtgttgccc aagtattaac actctatcct gaactggaac
     3121 aaatcgatat cgtggaaccg gatgagatgt tggtcgaggt ctgtcgtgag tatttcccag
     3181 actttgctgc agggctagat gatcctcgtg ttaccattta ctaccaaaat gggctacgct
     3241 ttttgcgaaa ctgcgaagat gattacgata ttatcatcaa cgatgcgaca gatccatttg
     3301 gccatacgga aggactcttt accaaggaat tctacggcaa tagttatcga gctctgaagg
     3361 aagacggcat catgatttac cagcatggga gtcccttctt tgacgaggat gagtcggcct
     3421 gccgaagcat gcaccgcaag gtcaatcaag cctttccaat cagtcgggtt tatcaggccc
     3481 atattccaac tagcccagct ggctattggt tgtttggatt tgcatcgaaa aaataccacc
     3541 ctgtcaaaga ttttgacaag gaaggctgga aaaaacgcca gcttttcaca gaatactaca
     3601 ctgcaaactt acacgtggga gcctttatgt tgcccaagta tgttgaggac attttagaag
     3661 aagaggaagg aaaaaaatga gtcgtttact agttattggt tgtgggggcg ttgcccaagt
     3721 tgctatttca aagatttgtc aagatagcga aacatttaca gagattatga ttgctagccg
     3781 taccaagtca aaatgcgatg acttgaaagc gaagctagaa ggcaaaacaa gtactaaaat
     3841 tgaaactgca gcacttgatg ctgacaaggt tgaagaagtg attgccctga ttggaagcta
     3901 caaaccagaa gctgttttga atgtagctct gccttatcaa gatttaacca ttatggatgc
     3961 ttgtttggca acaggtgttc actatatcga tacagccaac tacgaagcag aagacacaga
     4021 agaccctgag tggcgtgcta tctacgaaaa acgttgtaag gaacttggtt ttacagccta
     4081 ctttgactac tcatggcagt gggcttatca agagaaattc aaagaagcag gcttgactgc
     4141 tcttcttggt tctggttttg acccaggtgt aactagtgtc ttttcagctt atgccctcaa
     4201 acactatttt gatgaaatcc attatatcga cattttagac tgtaatggcg gtgaccacgg
     4261 ttatccattt gcaaccaact ttaatccaga aattaatctc cgtgaggttt ctgcgccagg
     4321 ttcttactgg gaagatggga aatgggtcga agtcgaagct atgtctatca agcgtgagta
     4381 tgatttccct caagttggac aaaaagatat gtatctcctt caccatgaag aaatcgaatc
     4441 attggccaag aacattccag gtgtcaaacg cattcgtttc tttatgactt ttggtcaatc
     4501 ttacttgacg cacatgaaat gtcttgaaaa tgttggactc cttcgtacgg ataccattaa
     4561 ctttaacggc caagaaattg ttccaattca atttttgaaa gccttgcttc cagatcctgc
     4621 tagccttggc ccacgtacag tcggaaaaac caatattgga tgtatcttta caggtgtcaa
     4681 agatggcgtt gaaaagacta tctacatcta caatgtttgc gaccatcaag agtgctatgc
     4741 agaagtcggg tcacaagcaa tttcatacac gacaggcgtt ccagccatga ttgggacaaa
     4801 attagtcatg aacggaactt ggaaacaagc tggagtgtat aaccttgagg agttagatcc
     4861 agatccattc atggaagctt tgaatgagta tggtttgcca tgggttgtgg ttgaaaatcc
     4921 acaaatggtg gactaatgaa gttagaacaa gtaccaacac cagcctatgt tattgacttg
     4981 gccaagttag aagctaattg ccgcattcta caatatgtac aagaagaggc cggttgcaag
     5041 gtcttgcttg cccagaaggc atattccctc tacaaaactt atcccttgat tagccagtat
     5101 ctatcaggta cgacagctag tggactctat gaggccaaat tggcaaggga agaatttcct
     5161 ggtgaagtcc atgtttttgc accagctttc aaggatgagg acatggagga attgcttggg
     5221 ataacagacc atatcgtctt taactcagag agacagttgc gtaagcatgg ggctcgttgt
     5281 cgagaggctg gtgtcagtgt tggtttgcgc ctcaaccctc agtgttcaac tcaaggagat
     5341 cacgcgctct atgacccttg tgcacctggt tctcgctttg gagttactat agacaagatt
     5401 ccgagtgatt tgctagattt ggttgacgga cttcattttc ataccctttg cgagcaggga
     5461 gcagatgatt tacaaacaac tttgaaagca gtagaagaac agtttggtcc ctacttacat
     5521 gaggtaaaat ggctcaatat gggtggtggt catcatatta caagagaagg ttacgatgtg
     5581 gatttgctga tttcagaaat caagcgtatc cgaaaaactt acaatcttga aatctatatc
     5641 gagcctggtg aagccattgc gcttaatgcg ggttatttag caactgaggt attagatatt
     5701 gtagaaaacg gtatggaaat cttggtttta gacgcctctg cgacctgcca tatgcctgat
     5761 gtacttgaga tgccctatcg tccacctttg agaaatggct ttgagtcaca ggaaaaagcc
     5821 catacctaca gactttcttc taatacctgt ctgactggcg atgtgattgg tgattatagt
     5881 tttgaaaatc cagtccaaat cggagacaga ctttattttc aagacatggc catttattct
     5941 tttgtcaaaa ataatacctt taatggtatt ggattgccaa gtctctatct catggacgaa
     6001 cagggagact gtagcttact caaagctttt ggctatcaag actttaaagg gagattatca
     6061 tgatggacag tccaaaaaaa ttaggctatc acatgccagc agagtacgaa ccccatcatg
     6121 gtaccctcat gatatggccg actcgaccag gatcatggcc ttttcaagga aaggctgcta
     6181 aaagagcatt tactcagatc atcgagacca tagcagaagg ggaaagagtc tatcttttgg
     6241 tggagcaggc ctatctatct gaagcccaat cctatcttgg agacaaggtt gtttatttag
     6301 acattcccac caatgatgcc tgggcgcgtg atactggccc aaccattctc gtcaatgata
     6361 aaggtaagaa attagccgtg gattgggcct ttaatgcttg gggaggcacc tatgatggtc
     6421 tttatcaaga ttatgaagag gatgaccaag tagccagtcg ttttgctgag gccttggaaa
     6481 ggcctgtcta tgatgctaaa ccttttgtac tggaaggagg cgcaatccat agcgatggtc
     6541 aaggaactat tctcgtaact gaaagttgct tgcttagtcc tggtcgcaat cctaacttga
     6601 ctaaagagga gattgaaaac acattattag aaagtcttgg tgctgaaaaa gttatttggc
     6661 ttccttatgg tatttatcag gatgaaacca atgaacacgt cgataatgtt gttgcctttg
     6721 ttggtcctgc tgaggttgtt ttggcttgga cagatgacga aaatgatccc cagtatgcca
     6781 tgtcaaaagc agatctcgaa ctcttagaac aggaaacaga tgcaaaaggt tgtcacttca
     6841 ccattcataa attgcctatc cctgcagttc gacaagttgt gacagaagaa gatttgccag
     6901 gctacatcta tgaagaagga gaagaagagc gatacgcagg tgaacgacta gcagcttcct
     6961 acgtaaactt ttatatcgcc aacaaggctg tcttggttcc acagtttgag gatgtaaacg
     7021 accaagtggc cttagatatc ctcagcaagt gtttcccaga ccgtaaagtt gtcggaatac
     7081 cagccagaga tattctctta ggtggtggca atatccactg tatcacccaa caaattccag
     7141 aataggagaa aaagatgaga aatgtaagag ttgcaaccat tcagatgcaa tgcgctaagg
     7201 atgtggcaac aaatatccaa accgcagagc gtttagtacg tcaggctgct gagcaaggag
     7261 cccaaattat tctcttgccc gagttgtttg aacatcccta tttctgtcag gaacgtcagt
     7321 atgactacta ccagtatgcc caatctgtag cggaaaatac tgccattcag cattttaagg
     7381 tgattgctaa ggaactacaa gttgttttac caatcagttt ctatgaaaaa gatggtaatg
     7441 tcttgtataa ctctattgcc gtcattgatg cagatgggga agtgctgggc gtttatcgaa
     7501 agacccatat accagatgac cattattatc aagaaaaatt ctatttcacg cctggtaaca
     7561 ctggtttcaa ggtctggaat actcgctatg ctaagattgg tatcggtatc tgttgggatc
     7621 aatggttccc tgaaacagcg cgctgtcttg cattgaatgg tgctgaattg ctcttttatc
     7681 ctacagctat cggttcagag ccaattttgg atacagatag ttgtggtcac tggcaacgta
     7741 ctatgcaagg gcacgcagca gcgaatattg ttccagtcat cgcagccaat cgttatggtt
     7801 tagaggaggt tactcctagt gaggaaaatg gcggacagag ttccagtctt gacttctacg
     7861 gttcctcctt tatgacggat gaaacaggag ctattctaga acgagctgaa agacaagaag
     7921 aagctgttct gttagctact tataacctag acaagggagc aagtgaacgc ctaaactggg
     7981 gcttgtttcg agatagaaga ccagaaatgt atagacaaat tacagattag tgtgggagaa
     8041 atgagagatt cattctgcta gactaacttc ttattagcaa ctataagata ctatggcatc
     8101 tagtaaatcg atttttatga ttcgctattc ttctctattg attagtccgt attttaaaat
     8161 attagcaaaa aagcaaatag cagtaacttc tgtctatttg cttttctttt ttatagaata
     8221 tatttctcaa tagcacgcgc aacgccgtct tcttcgttgc ttgaggtaac ggcatccgca
     8281 agagatttga cataatcgct ggcatttccc attgcaatcc caagccctgc aaactggagc
     8341 atttcgatat cgttattagc atcgcccatg gccataatct ctgaggaatc aatcttcaaa
     8401 atctcagcta gtcgtgaaag agcagtagcc tttgtcgttc caagtggcat tgcttcataa
     8461 atgacaggct gcgaacgaac tccactgaat cgttggcaaa gctcttcagc aaaacgctgc
     8521 tcaaaatcgt ctgtttgttc ttttgttcct aaaaacatac cttggaacat ccggaacttt
     8581 ccactagtcg cttcctcaag agaaatttca gtcaggtctg aaaatactag tttagcatca
     8641 ttttcaataa cttgattggg ctttccaccg agaacaaaat aatgtgactc gtcaaaaagt
     8701 gtcaactgaa catcactctt ttcagcaagg tcatagaggt attcgatgtc agctggacta
     8761 agttcttgcc agtcaaccaa gccccagtcg ctagtctggt gagttgaaca accgttgtta
     8821 acaatcacat actcattctg gaggtcgagt cccagttttt tgtagtaggg gaggacaccg
     8881 aaaagggggc gacccgtaca gagaaccagt ttgacacctt tttcaatggc tttgtgaata
     8941 gcagtaatgt gagcttgtgg gatttccttg gcttcattga ggagggtgcc gtccatatcc
     9001 aaggctagta gtttaatcat aggtcttcct tctttatctt ttgctattat tatagcatat
     9061 tttggagaag aaattgatag aaagcttgag actaattgat tttatagttt aagatgtttt
     9121 gatgacaact catgatttga agaggatatt tcgcaaagat atgctatact atgtttgtca
     9181 atgttgcaac tagacaaatt
//
LOCUS       DAVKZB010000072         9048 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKZB010000072 DAVKZB010000000
VERSION     DAVKZB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.072"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            117..3218
                     /locus_tag="VXA80_001471"
     CDS             117..3218
                     /locus_tag="VXA80_001471"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV0353951.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLV
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAGQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKHPLLEPLVKV
                     GLFDLFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVRKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQKIREAVAERFWIQ
                     VKNHDSEQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3300..4307
                     /gene="pfkA"
                     /locus_tag="VXA80_001472"
     CDS             3300..4307
                     /gene="pfkA"
                     /locus_tag="VXA80_001472"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV0353952.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4366..5871
                     /gene="pyk"
                     /locus_tag="VXA80_001473"
     CDS             4366..5871
                     /gene="pyk"
                     /locus_tag="VXA80_001473"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV0353953.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6187..6435)
                     /locus_tag="VXA80_001474"
     CDS             complement(6187..6435)
                     /locus_tag="VXA80_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0353954.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(6601..7362)
                     /locus_tag="VXA80_001475"
                     /pseudo
     CDS             complement(6601..7362)
                     /locus_tag="VXA80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7674..8546
                     /locus_tag="VXA80_001476"
     CDS             7674..8546
                     /locus_tag="VXA80_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV0353955.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8692..9036)
                     /locus_tag="VXA80_001477"
                     /pseudo
     CDS             complement(8692..9036)
                     /locus_tag="VXA80_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2754 a         1576 c         1948 g         2770 t
ORIGIN      
        1 caaaaataca aatgaataga aaataaatta gtaagctgat gaaatttttc tcaagagaag
       61 ctatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag tgtaaattga
      121 tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt tcgatcgaaa
      181 agtatgtgag agcagctaaa gaatacggct acactcattt ggctatgatg gatattgaca
      241 atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt catcctttgc
      301 tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc tttttagctc
      361 tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg cagggggaga
      421 aaacttggtc agtcctgtcc cagtacctgg aggatatcgc ggtcattgtg ccttattttg
      481 atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat ccagaaacac
      541 tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct tttgaaagca
      601 gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg ctcagagaag
      661 ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag aaactattcc
      721 aagagcgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc atttcttacg
      781 acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca gtagaggagt
      841 tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa gaatatcaag
      901 atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat tatttcttgg
      961 ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga atgggaaggg
     1021 gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt gacccagtag
     1081 agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg cctgatattg
     1141 atattgatat cccagatatt tatcgtccag attttatcag atatgttggt aataaatatg
     1201 gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag caagctcttc
     1261 gagatgtctt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt actaagaaaa
     1321 tcagttttcg tgacaatctt aagtcggcct atgagggcaa tctccagttt cgtcagcaaa
     1381 tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata gagggctatc
     1441 caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat ttgaccaact
     1501 acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct catggagttg
     1561 aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc tttgtccaga
     1621 agatgcaaga gttgcttgtt gaaacagaag gtattcacct taaaattgaa gaaatagatt
     1681 tggaagacaa agaaacgtta gatttatttg cctctggaaa tacaaaaggt atctttcaat
     1741 ttgagcaacc tggtgctatt cgcttgctca aacgtgttca accagtctgt tttgaagatg
     1801 tcgtagcaac tacttctcta aatcgaccag gtgcaagtga ctacatcaat aattttgtgg
     1861 caaggaagca tgggcaggaa gaagtgactg ttctagattc agctctggag gatattttgg
     1921 ctccaactta tggcattatg ctctatcagg agcaggttat gcaggttgct cagcgatttg
     1981 ctggatttag tcttgggaag gccgatattt tgcgtcgagc tatgggtaaa aaggatgcct
     2041 ctgctatgca tgagatgagg gcttccttta ttcaaggctc catagaagca ggccatactg
     2101 cggaaaaagc agggcaggtt tttgatgtta tggagaagtt tgcaggctat ggttttaaca
     2161 ggtcacacgc ctatgcctac tcagcattgg ccttccagtt ggcttatttt aaaacacatt
     2221 atccagccat attttatcag attatgttga attctgccaa cagtgattat gtaacagatg
     2281 ccttagaagc aggttttgaa gtagccccat tgtccatcaa tactattccc tatcacgata
     2341 aaattgccaa caagtctatc tatataggtt tgaaatcgat taaggggctc agcaaggact
     2401 tggcgctttg gattattgaa catagacctt attctaatat tgaagatttt atagctaaat
     2461 tacctgagaa ttatctgaaa catcctctgc tagaaccttt ggtaaaagtt ggcctttttg
     2521 atttatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta tttgaatttg
     2581 tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa tcggaagatt
     2641 ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt gtcagcaaac
     2701 atccactaca agctattgca agtaaggcta tttacccgat taccccaatc ggaaatttgt
     2761 cagaaaatag ctatgctatt attttggttg aagttcggaa aataaaagtg attcgtacca
     2821 aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa ttggatgtca
     2881 ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga gccttctact
     2941 atgtaaaagg aaaaatacaa tcacgtgatg gccgtctgca aatgattgca caaaaaataa
     3001 gagaagcagt tgctgaacgc ttttggattc aggttaaaaa tcatgactcg gagcaagaga
     3061 tgtcacgtat tttagaccag tataaaggcc caatcccagt catcatccgg tatgaagagg
     3121 aacagaaaac catcgtttct ccccatcatt ttgtagctaa atctgatgaa ttagaggcga
     3181 aattgaatgg aatagttatg aaaacgattt atcgctaaaa ttacggaaaa tagaagaatt
     3241 ttcaacgtaa atgtggtata atcagtaaga atgttaaaag aaaaaggagc ataaccaata
     3301 tgaaacgtat tgctgttttg actagtggtg gagacgcccc tggtatgaac gctgccatcc
     3361 gtgcagttgt tcgtcaagca atttcagaag gaatggaagt ttttggtatc tatgacggat
     3421 atgctggtat ggttgccggt gaaattcatc ccctagatgc agcttcagta ggggacatca
     3481 tttctcgtgg tggtactttc cttcactcag ctcgttaccc agagttcgct caacttgaag
     3541 ggcaacttaa agggattgag caattgaaaa aacacggaat tgaaggtgta gttgttatcg
     3601 gtggtgacgg atcttaccac ggcgctatgc gtttgactga acatggcttc ccagctattg
     3661 gtcttccagg tacaatcgat aacgatatcg ttggtactga ctttacaatc ggttttgaca
     3721 cagcggttac tactgccatg gacgctattg ataagattcg tgatacatca tcaagtcacc
     3781 gtcgtacttt tgtaatcgaa gttatgggac gtaacgctgg tgatatcgct ctttgggctg
     3841 gtattgcaac tggtgctgat gaaatcatca tccctgaagc aggcttcaag atggaagata
     3901 tcgtagcaag catcaaagct ggttatgaat gtggtaaaaa acacaatatc atcgtcttag
     3961 ctgaaggtgt gatgtcagcg gctgaatttg gtcaaaaact taaagaagct ggagatacaa
     4021 gcgaccttcg tgtaacagaa cttggacata ttcaacgtgg tggttctcca actgcgcgtg
     4081 accgtgtttt ggcgtcacgt atgggtgcac atgctgttaa acttcttaaa gaaggtatcg
     4141 gtggtgttgc ggttggtatt cgtaacgaaa aaatggttga aaatccaatt cttggtactg
     4201 cagaagaagg ggcattgttt agccttactg cagaaggtaa gattgtggtt aacaacccgc
     4261 acaaagccga tattgagcta tctagcttga ataagagctt gtcataattt acaatttagt
     4321 taataagacc aaaaaggtca tatatataaa ggagtcacaa aaatcatgaa caaacgtgta
     4381 aagatcgttg caactttggg tcctgcggta gaaatccgtg gtggtaaaaa attcggtgag
     4441 gatggatact ggggtgaaaa acttgatgtt gaagcttcag ctaaaaacat tgctaaattg
     4501 attgaagctg gtgctaacac attccgattc aacttctcac acggcgacca ccaagaacaa
     4561 ggtgagcgta tggcaactgt taaacttgcg gaaaaaattg caggtaaaaa agttggtttc
     4621 cttcttgata caaaaggacc tgaaatccgt acggaattgt tcgaaggtga agctaaagaa
     4681 tattcataca aaactggtga aaaaattcgt gttgcaacta aacaaggaat caaatcaact
     4741 cgtgaagtga ttgcgttgaa cgttgctggt gctcttgata tctatgatga tgttgaagtt
     4801 ggtcgtcaag ttttggttga cgatggtaaa cttggtcttc gtgtggttgc taaagatgat
     4861 gcaactcgtg aatttgaagt tgaagttgaa aacgatggta tcatcgctaa acaaaaaggt
     4921 gtgaacatcc ctaacactaa aattcctttc ccagctcttg ctgaacgcga taacgacgat
     4981 atccgtttcg gtcttgaaca aggtatcaac ttcatcgcaa tttcattcgt acgtactgca
     5041 aaagatgtga acgaagttcg tgcaatctgt gaagaaactg gaaacggaca tgttcaattg
     5101 ttcgctaaaa tcgaaaacca acaaggtatc gataacttag acgaaatcat cgaagcagct
     5161 gatggtatta tgattgctcg tggtgatatg ggtatcgaag taccgttcga aatggttcca
     5221 gtttatcaaa aaatgattat caagaaagtc aatgctgcag gtaaagttgt tatcactgca
     5281 acaaacatgc ttgaaacaat gactgaaaaa ccacgtgcaa ctcgttcaga agtatcagac
     5341 gtattcaacg ctgttatcga cggaactgac gctacaatgc tgtcaggcga gtctgcaaac
     5401 ggtaaatacc cactcgagtc agtaactaca atggctacaa tcgacaagaa cgctcaagct
     5461 cttcttaatg aatacggacg tcttgattca gattcatttg agcgtaactc taagacagaa
     5521 gtaatggctt ctgctgttaa agatgctact agctcaatgg atatcaaatt ggttgtaact
     5581 cttactaaga caggtcatac tgcacgtttg atttctaaat accgtccaaa tgctgacatc
     5641 ttagcattga catttgacga attgacagaa cgtggcttga tgttgaactg gggtgttatc
     5701 ccaatgttga cagatgctcc atcttcaact gacgatatgt tcgaaatcgc tgaacgtaaa
     5761 gcggtagaag caggtctcgt tgagtcaggc gatgatatcg ttatcgttgc tggtgtgcca
     5821 gtaggagaag ctgttcgcac aaacacaatg cgtatccgca cagtacgtta agaaaaatat
     5881 aaaaacctat catatccagc tttagagctt gtgtgatagg ctttttgtat agagggtaag
     5941 aaataggcaa aactttcata atggattgat actcttcgaa aatctcttca aaccacgtca
     6001 gcgtcgcctt accgtacata tgttactgac ttcgtcagtt ctatctacaa cctcaaagca
     6061 gtgctttgag caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatga
     6121 aataagatat gcacaaattg attagaaagt caaatgaatt tctacaaatg ttttagcaat
     6181 cgcaatctac ttgtctagat tcgatctgat atattttcga tttaatgata tggtatttaa
     6241 aacctccaaa gtagcttgct ccattctttt acttacgtga gtgtagatgt tatttactgt
     6301 tttagcgttt ttgtgttcca ctctaaccat tatagcattc ttctcagcta gtgtactaag
     6361 gagtgtgtgc ctgaaaatat gggaactaag gggctggttt atcggtttct ctagtttagt
     6421 atttgccttt tgcaaagtga tcttaaatgc ctttctctaa atttacatat cactattgtt
     6481 taacaaaatc taatctattt taggtcactt attctttttt tgaaatgtag aatgaacttt
     6541 ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc tccccctttt
     6601 ttaattctcc ctatatagcc tgacaacttt tccgatggta cgaatatggt tgctttcgtc
     6661 taggtggatg tcggagtatt cgggattgag tttttttgag gtagccttgg cggagtttct
     6721 tgacatagtt agtgccgtct acttggaaga tgccgatggt attatagtca atctgtgggg
     6781 tattcttgat aaataggtag tcgctgtttc ttatctttgg ctccatggac ttgctgacga
     6841 cataagcgat tgggtcgtag tcgtctggga taatggaaac tccataacta aatcgtcgtc
     6901 ctgcatcaag cggctacctg cagagataaa ctacctaaca cgagagtaag tagtctgtct
     6961 gtagtcgtcc agtctgatga tttttacgat acttcgtttt tctgatcata cagttgcctc
     7021 tcggcatagg tcaggacttt accttgtctg ggtggtaccc gttggtcgta gatagattgg
     7081 atatcgctag gagaatcctt ttgaactgga ggaaagaggg catcaatcaa gctactgaat
     7141 actttaacta agtcaaatat agtatttttc ttagtagacc taaccctttt ttcataattt
     7201 ctaatggtgt ttttacttat acctatctta gtacccaatt cttattgagt ccaactatta
     7261 ctagtctata ttgttttata gttgattgag tttggaatag tacgctgtag ctgctaaaac
     7321 atttctagga attaatttgc ctttcctaat agagttgttc atatcttatt tcaatctatt
     7381 atgtttttca cctctaacaa tcgcaatctc ttctttatct atgaatgaaa tcgatttcta
     7441 tttttgtaag taaagcataa cacgaaatcc acgaaaatga aaacctttgt tgtgttttcg
     7501 taaaaaattt gttgacagag cacggaacgc ctactataat taaatcaagc ttaaggaaat
     7561 aacaaaaaaa caaatcggag ggaaagacga tgaacacatt aaatgagaaa gtaatcaata
     7621 tctgtaaagc agtagttaaa gaaactttaa tccaagacat ttaggagaaa gaaatgaaga
     7681 aatcaagaaa attagccaca ttaggaatct gttcagcttt gtttttagga ttggctgctt
     7741 gtcaacaaca acatgctact tctgagggga cgaatcaaag gcaaagcagt tcagcgaaag
     7801 ttccatggaa agcttcatac accaacctaa acaaccaggt aagtacagaa gaggtcaaat
     7861 ctctcttatc agctcacttg gatccaaata gtgttgatgc attttttaat ctcgttaatg
     7921 actataatac cattgtcggc tcaactggct tatcaggaga tttcacttcc tttactcaca
     7981 ccgaatacga tgttgagaaa atcagtcatc tctggaatca aaagaagggc gattttgttg
     8041 gaaccaactg ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc attccaaagc
     8101 ttgaaaagaa tgaccagttg cttttcctag ataatgatgc gattgataaa ggaaaggtct
     8161 ttgattcaca agataaggaa gagtttgata ttctattttc gagagttcca actgaggcaa
     8221 ctacagatgt caaggttcac gctgaaaaga tggaaacatt cttctcacaa tttcaattca
     8281 atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa tctggatggc gagtatctgt
     8341 ttgtaggcca cgttggggtc ttagtacctg ctgatgacgg tttcttattt gtagagaaat
     8401 tgactttcga agagccctac caagcgatta aatttgctag taaggaagat tgctacaagt
     8461 atttgggcac caagtatgcg gattatacag gcgagggact ggctaagcct tttatcatgg
     8521 ataatgataa gtgggttaaa ctttaatagt gttatgatat gacagtaatc ttaggatttt
     8581 tatttctatt tttgatgact tggtgggtta tttcaatcgt taatagccag aaaccacgca
     8641 aaaatgaaac atttattggc tatatccaaa gatacgatat tgatgggaat ggctatcaat
     8701 cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaaaactac tttgaacaag
     8761 agttacttca acccattggc tccgacggat taagttgctt tcgtgaacac caaaatcagc
     8821 cgcaatttct tcataagtgc ggtattctcg cacatattga agagtggcca taagaaggtc
     8881 ttctaggctt aatttaggtt ttcgtccccc ttttgtgtgt ttaagttgat aagcagtttt
     8941 taatacagct aacatctctt taaaagtggt gcgctgaaca ccaacaagac atttaaatcg
     9001 tgcatcagtt agttgcttac ttgcttcata attcatagaa ctactata
//
LOCUS       DAVKZB010000073         9047 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKZB010000073 DAVKZB010000000
VERSION     DAVKZB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.073"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            14..205
                     /gene="secG"
                     /locus_tag="VXA80_001478"
     CDS             14..205
                     /gene="secG"
                     /locus_tag="VXA80_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV0353956.1"
                     /translation="MIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEAVMQRLTG
                     ILVFFWLAIALALTVLSSR"
     gene            307..2661
                     /gene="rnr"
                     /locus_tag="VXA80_001479"
     CDS             307..2661
                     /gene="rnr"
                     /locus_tag="VXA80_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV0353957.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLCIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNCERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2624..3091
                     /gene="smpB"
                     /locus_tag="VXA80_001480"
     CDS             2624..3091
                     /gene="smpB"
                     /locus_tag="VXA80_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV0353958.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3107..3967
                     /gene="tehB"
                     /locus_tag="VXA80_001481"
     CDS             3107..3967
                     /gene="tehB"
                     /locus_tag="VXA80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV0353959.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4040..4993
                     /gene="coiA"
                     /locus_tag="VXA80_001482"
     CDS             4040..4993
                     /gene="coiA"
                     /locus_tag="VXA80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV0353960.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5012..6814
                     /gene="pepF"
                     /locus_tag="VXA80_001483"
     CDS             5012..6814
                     /gene="pepF"
                     /locus_tag="VXA80_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0353961.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYVDLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6816..7529
                     /locus_tag="VXA80_001484"
     CDS             6816..7529
                     /locus_tag="VXA80_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV0353962.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQRLFDATLDNSELTATLVPLG
                     DGILMLRKNVADVQLPDSE"
     gene            7596..8537
                     /gene="prsA"
                     /locus_tag="VXA80_001485"
     CDS             7596..8537
                     /gene="prsA"
                     /locus_tag="VXA80_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV0353963.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8654..8995)
                     /locus_tag="VXA80_001486"
                     /pseudo
     CDS             complement(8654..8995)
                     /locus_tag="VXA80_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2906 a         1674 c         1980 g         2487 t
ORIGIN      
        1 agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa accaatccag
       61 caatgtattt gatgccagtt caggtgattt gtttgaacgc agtaaagctc gcggttttga
      121 agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca ttgccttagc
      181 attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct ttgcctcttt
      241 ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa aatctagaaa
      301 gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt gactgttaat
      361 gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt gattaaaacc
      421 ttgtccttaa tggaaagaaa gcaccaaatt cgttttgaag aagatggtag tctgacatta
      481 gaaattaaga aaaaacatga gattaccctc aaggggattt ttcatgccca taaaaatggc
      541 tttggctttg ttagtctgga aggcgaggag gacgaccttt ttgtagggaa aaatgatgtc
      601 aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc tgaccgcaat
      661 aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt gacaacagtt
      721 gtcgggcaaa tcgttctgga tcaggaaaaa cctaagtatg ctggctatat tcgttcaaaa
      781 aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaaatt agaaggaaca
      841 gaagttctca aagtctttat cgataaatac ccaagcaaga aacatgattt ctttgtcgcg
      901 agtgttctcg atgtagtggg acactcaacg gatgtcggaa ttgatgttct tgaggtcttg
      961 gaatcaatgg acattgtatc cgagtttcca gaagctgttg ttaaggaagc agaaagtgtg
     1021 cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga tgaaattacc
     1081 tttaccattg acggtgcgga tgccaaggac ttggacgatg cagtgcatat caaggctctg
     1141 aaaaatggca atctggagct tggggttcac atcgcagatg tttcttatta tgtgaccgag
     1201 gggtctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt gacagaccga
     1261 gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa tccccaagtt
     1321 gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt ggtcaactat
     1381 accattacac aaacagttat caagaccagt tttcgtatga cctatagcga tgtcaatgat
     1441 atcctagctg gcgatgaaga aaagagaaaa gaatatcata aaattgtatc aagtatcgaa
     1501 ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg agctctcaat
     1561 tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt tgatatcgtt
     1621 cttcggcagc gtggtattgc cgagcggatg attgagtctt ttatgttgat ggctaatgaa
     1681 acagttgccg aacatttcag caagttggat ttgcctttta tctatcgaat tcacgaggag
     1741 cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg cttgtgcatt
     1801 tatggaactg ccagtgagat tagtcaggag gcacttcaag acatcatgcg tgctgttgag
     1861 ggagaacctt atgcagatgt attgtccatg atgcttcttc gctctatgca gcaggctcgt
     1921 tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac tcactttacc
     1981 agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga ttacggccgt
     2041 tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac ccagtcttcc
     2101 aactgtgaac gtcgtgccat agaagctgag cgtgaagtcg aagccatgaa aaaggctgag
     2161 tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat tgtcaaattc
     2221 ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac taatctacct
     2281 gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc aggtatcact
     2341 ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat gactggagag
     2401 attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt gaaacagtct
     2461 agtcatagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga agacaagaga
     2521 aaatcaggac gctcaaatga taagcgtaag cattcacaaa aagacaagaa gaaaaaagga
     2581 aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa agggcgaggg
     2641 aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag atacgctaga
     2701 ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc gaattaatct
     2761 caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagcaatg ttcatatcgc
     2821 gccttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta aactcctgct
     2881 ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa tgaccttagt
     2941 tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac ttgccaaagg
     3001 gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc gagatatcgc
     3061 gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg aaaaattagt
     3121 tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagctg ttcagcaaaa
     3181 gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag ctctcaagtt
     3241 tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag caggggcaga
     3301 caatccaatg gcccaacctc aagcctggca ccgagtggaa gctgccacag atgatgtgga
     3361 atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat acaataccaa
     3421 tcctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga aagctttgga
     3481 tttgggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt ttgatgtgac
     3541 ggctgtagat caaaatggac tagctcttga aatcttgcaa agcattgtgg agcaggaaga
     3601 tttggacatg cctgttggcc tttacgatat caattcagct agcattgaac aagaatatga
     3661 ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc cagctattat
     3721 tcaaaatatg caggagaaaa ccagtgttgg tggttacaac cttatcgttt gtgccatgga
     3781 cacggaggat tatccttgct cggttaactt cccattcacc tttaaagaag gagaactggc
     3841 agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc atttgcaccg
     3901 tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag ctaagaaaat
     3961 caagtaaaca tacatgaaga ttaggaattt tcctgatctt ttttcttttt tacgaatgat
     4021 atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat tggtaaatgt
     4081 gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag gccagatcca
     4141 tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatccttaa aagactgtga
     4201 ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc tctatcactg
     4261 gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta aacagattgc
     4321 ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct tgcctcagaa
     4381 agtccttaaa gagcgaagtg agggctatcg tagtcagggt taccaagtac tgtggttgct
     4441 gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt ttctttattt
     4501 cagtcaaaac atgggctttt atgtttggga attagacaag gaaaaacaag ttttaagact
     4561 caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca aggaattttc
     4621 ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc tataagaaac aaaaaatatc
     4681 tcattttaca gtttctgagg acaaggacat ctgtcgctat atccggcaac aactttatta
     4741 tcaaaatctc ttttggatga aagaacaagc agaagcctat caaaagggag aaaatatcct
     4801 gacttatgga ctgaaagaat ggtatccaca aattcgacca atagtgggca aatttttcca
     4861 gattgaacaa gacttgacta gctattatca gcacttttat acctattacc aaaaaaatcc
     4921 tcaaaatgat tggcaaaagc tttatccacc agccttttat cagcaatatt tcttgaaaaa
     4981 tatggtagaa tagaaaggat ggaggaatct aatggtatta caaagaaatg aaataaatga
     5041 aaaagataca tgggatctat caacgatcta cccaactgac caggcttggg aagaagcctt
     5101 aaaagattta acagaacaat tggagacagt agcccagtat gaaggccatc tcttggatag
     5161 tgcggataac ctactagaaa tcactgaatt ttctcttgaa atggaacgcc agatggagaa
     5221 gctttacgtt tatgctcata tgaagaatga ccaggataca cgtgaagcta agtatcaaga
     5281 gtactatgcc aaggccatga cactctacag ccagttagac caagcctttt cattctatga
     5341 gcctgaattt atggagatta gcgaaaagca gtatgctgac tttttagaag ctcaaccaaa
     5401 gctgcaggtt tatcaacact attttgacaa gctcttgcaa ggcaaggatc acgttctctc
     5461 acaacgtgaa gaagaattat tggctggagc tggagaaatc tttggttcag caagtgaaac
     5521 cttcgctatc ttggacaatg cggatattgt gttcccttat gtcctagacg atgatggtaa
     5581 agaagttcag ctatctcatg ggatttacac acgtttgatg gagtctaaaa aacgtgaggt
     5641 tcgccgtggt gcctatcaag ctctttatgc gacttacgaa caattccaac acacctatgc
     5701 caaaaccttg caaaccaatg ttaaggtgca aaattaccgt gctaaagttc gtaactacaa
     5761 gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca gaaagtgttt atgacaattt
     5821 ggtagcagca gttcgcaagc atttgccact cttacatcgc tatcttgagc ttcgttcaaa
     5881 aatcttgggg atttcagatc tcaagatgta cgatgtctac acaccgcttt catctgttga
     5941 atacagtttt acctaccaag aagccttgaa aaaagcagaa gatgctttgg cagtcttggg
     6001 tgaggattac ttgagccgtg tcaaacgtgc cttcagcgag cgttggattg atgtttacga
     6061 aaatcaaggc aagcgttcag gtgcctactc tggtggttct tacgatacca atgcctttat
     6121 gcttctcaac tggcaggaca atctggacaa tctctttact cttgttcatg aaacaggtca
     6181 cagtatgcat tcaagctata ctcgtgaaac tcagccttat gtttacggag attactctat
     6241 ctttttggct gagattgcct caactaccaa tgaaaatatc ttgacggaga aattattgga
     6301 agaagtggaa gacgacgcaa cacgctttgc tattctcaat aacttcctag atggtttccg
     6361 tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa cacgccattc accaagcaga
     6421 tcaaaatggg gaggtcttga caagcgattt cctaaataaa ctctacgtag acttgaacca
     6481 agagtattat ggtttgagta aggaagacaa tcctgaaatc caatacgagt gggctcgcat
     6541 tccacacttc tactataact actatgtata tcaatattca actggatttg cggccgcctc
     6601 agccttggct gaaaaaattg tccatggtag tcaagaagac cgtgaccgct atatcgacta
     6661 cctcaaggca ggtaagtcgg actatccact taatgtcatg agaaaagctg gtgttgatat
     6721 ggagaaggaa gactacctca acgatgcctt tgcagtcttt gaacgccgtt taaatgagtt
     6781 tgaagccctt gttgaaaaat taggattggc ataaaatggt tgaatcgtat agtaagaatg
     6841 ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat tgtagacttg atgcgtcagc
     6901 gtcaaaagca ggtcacaggt tccttgaaag aattggaaga ctttgcccgc aaggaaaata
     6961 ttcctattat tccccatgaa acggttgctt atttccgttt tcttatggaa accatgcagc
     7021 ctaaaaatat tctggaaatt gggacggcta tcggtttttc agctctcttg atggctgaac
     7081 atgcgccaaa tgctaagatt acaactattg atcgtaatcc agaaatgatt ggttttgcca
     7141 aggaaaattt tgcccagttt gacagtcgca agcaaatcac tctcctagag ggagatgcgg
     7201 tggatgtctt atctacactg acagagtctt atgatttcgt ctttatggat tctgccaagt
     7261 ctaaatacat cgtctttctg ccagaaattc tcaaacattt ggaagttggt ggtgtggttg
     7321 tcttggatga tattttccaa ggcggtgatg ttgccaagga cattatggaa gtccgtcgag
     7381 gtcagcgaac catctatcga ggccttcaaa gactatttga cgcaacctta gacaattcag
     7441 aactcaccgc aacattagtg cctttaggag atggtattct catgcttcgt aaaaatgtag
     7501 cagatgttca attgcctgac agcgaatgat tttcagaaaa atttaagaaa aaatagtaaa
     7561 atagatagag taacacttat ctcaaaggag tagacatgaa gaaaaaatta ttggcaggtg
     7621 ccatcacact attatcagta gcaactttag cagcttgttc gaaagggtca gaaggagcag
     7681 accttatcag catgaaaggg gatgtcatca cagaacatca attttatgag caagtgaaaa
     7741 acaacccttc agcccaacaa gtcttgttaa atatgaccat ccaaaaagtt tttgaaaaac
     7801 aatatggctc agagcttgat gataaagagg ttgatgatac tattgccgaa gaaaaaaaac
     7861 aatatggcga aaactaccaa cgtgtcttgt cacaagcagg tatgactctt gaaacacgta
     7921 aagctcaaat tcgtacaagt aaattagttg agttggcagt taagaaggta gcagaagctg
     7981 aattgacaga tgaagcctat aagaaagcct ttgatgagta cactccagat gtaacggctc
     8041 aaatcatccg tcttaataat gaagataagg ccaaagaagt tctcgaaaaa gccaaggcag
     8101 aaggtgctga ttttgctcaa ttagccaaag ataattcaac tgatgaaaaa acaaaagaaa
     8161 atggtggaga aattaccttt gattctgctt caacagaagt acctgagcaa gtcaaaaaag
     8221 ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat tacagcaact ggcacacaag
     8281 cctacagtag ccaatattac attgtaaaac tcactaagaa aacagaaaaa tcatctaata
     8341 ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac tcaaaaacaa aatgattcaa
     8401 catttgttca aagcattatc ggaaaagaat tgcaagcagc caatatcaag gttaaggacc
     8461 aagccttcca aaatatcttt acccaatata tcggtggtgg agattcaagc tcaagcagta
     8521 gtacatcaaa cgaatagtcc aaatcaatga atcagggaaa aaactcgact tcaggaaaaa
     8581 atgaagcaaa cattcccaca ataaaacgcg tagtacaagg tttgtactga cccccaaaag
     8641 ttagacaaat aatttatccg aaggatttag ttctgtattg cacagggcta agtcctttta
     8701 gttttacctt aattctcttg ttgttgtaat aatcaatata gtctataatg gcttgttcca
     8761 attgattaag tgatttaaat gttttctcat agccataaaa catttcggat tttaaaatgc
     8821 caaagaaaga ttccatccta ccgttgtctt ggctgtttcc tttgcgtgac atagatgctt
     8881 gaattccctt actctctagg aagcgatgat aagaatcgtg ttggtattgc cagccttggt
     8941 cactatggag aatcgtattc tcgtagtgct tctctttgaa tgcctgttcc aacattaacg
     9001 atcaatcaat ttaatcatgt acctaatatt agaattgttt atcccaa
//
LOCUS       DAVKZB010000074         8978 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKZB010000074 DAVKZB010000000
VERSION     DAVKZB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.074"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..469)
                     /locus_tag="VXA80_001487"
     CDS             complement(<1..469)
                     /locus_tag="VXA80_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV0353964.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVT"
     gene            591..1337
                     /locus_tag="VXA80_001488"
     CDS             591..1337
                     /locus_tag="VXA80_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0353965.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNMATLENAGIMVKVVS"
     gene            1350..2330
                     /locus_tag="VXA80_001489"
     CDS             1350..2330
                     /locus_tag="VXA80_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV0353966.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            2525..2845
                     /locus_tag="VXA80_001490"
     CDS             2525..2845
                     /locus_tag="VXA80_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0353967.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            2891..3199
                     /locus_tag="VXA80_001491"
     CDS             2891..3199
                     /locus_tag="VXA80_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0353968.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3216..4514
                     /locus_tag="VXA80_001492"
     CDS             3216..4514
                     /locus_tag="VXA80_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0353969.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            4665..7103
                     /locus_tag="VXA80_001493"
     CDS             4665..7103
                     /locus_tag="VXA80_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV0353970.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7122..7790
                     /locus_tag="VXA80_001494"
     CDS             7122..7790
                     /locus_tag="VXA80_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV0353971.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            7808..8896
                     /locus_tag="VXA80_001495"
     CDS             7808..8896
                     /locus_tag="VXA80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV0353972.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         2956 a         1259 c         1826 g         2937 t
ORIGIN      
        1 cagtcacttt tttatgtttt atagaattat aatgttttag gtctgtgtag ataaaatcca
       61 catattgaat taaatcaata aatttttcat gatcaacaaa ggcagtagtt tcaatggcag
      121 tgtgtatgtg atgttcttta gctgatttta agatggcttt agcaaattca aactgagcaa
      181 atatttcacc tcctgataaa gttaaacctc caccggattc ttcgtaaaat tctttgtctt
      241 ttaatacctc tgtaataatt tcttctacag tcttttcttc accgactaag gtgaatttct
      301 ctcgttgagc atctttcatt ttttcaggtt tcattctttg agattcagga ttagaacacc
      361 atggacagcg cagaggacat ccttttaaaa aaacagttgt acgaataccc ggaccgtcat
      421 gaattgaaaa gtgttgaata ttaaaaataa ttcctttaga tatttccata agagttcctc
      481 ctcttcataa gtctatccta tcacaaaaac gaaagaaaaa caattacgaa cgaaaggtta
      541 atttgttgtt ttttgctttt tattttggta gaataaaacg gaggtgtaaa atgaaaagat
      601 tggaacaaat tattaaatta gtatcagaat atgaaaagat cgacgttaat acattatcgg
      661 aaaaattaaa tgtatcgaaa gtaacgatta gaaaagattt agataaatta gagtcaaaag
      721 gtttattaca cagagagcat ggatatgctg tattaaatag tggagatgac ttaaatgtac
      781 gtttgtcaat taactatgaa attaagagaa aaattgttca ggaagcagta aaattggtgt
      841 cagataatga aacaataatg atagaatctg gatcgacctg tgctttactt gctgaggaaa
      901 tttgcaagca aaaaagaaat gttacgattg taacaaattc attttttata gcaaattttg
      961 tgagagctta tgattcatgt cgtattattg ttcttggtgg agagtttcag aaggattcac
     1021 aggttactgt gggaccttta ttaaaagaaa tgatacagac ttttcatgtg cgtcaagctt
     1081 ttgttgggac agatggctac gataaagaga tgggctttac aggaaaagat ttaatgcgca
     1141 gtgaggtagt tcaatatatt tcagcagcgt cggataaagt cattgtgcta actgactcaa
     1201 gtaaatttga taaaaggggt acagtaagaa gatttgcttt aagtcaagtc tatgaagtaa
     1261 taacagacga aaaactttct aaacaaaata tggctacatt agaaaatgct gggataatgg
     1321 ttaaggtagt ttcgtaagag gttaagtgta tgaatcaaga taggaataaa ctgctctcta
     1381 aaattgctta tctgtattat attgaaaact taaatcagtc acaaatagca gcaaaattag
     1441 gaatttatag aacctctatt agtagaatgt taacagaagc aaggaatgca ggaattgtta
     1501 aaattgaaat agagaatttt gataccaata tgtttaagtt ggaaaattat gtaaaagaaa
     1561 aatatggtct ggaaagttta gaaattattc caaatgaatt tgatgataat ccaacaattt
     1621 tatctgaaag aatttctcaa gttgcagcag gtgtccttag gaatctaatt gatgataata
     1681 tgaaaattgg cttttcttgg gggaaaagtt taagtaattt agtagattta attcacagta
     1741 aaagtgtccg aaatgttcac ttctatcctc tagcaggtgg tcctagtcac atacacgcta
     1801 aataccatgt gaatacactg atttatgaaa tgtctagaaa atttcatgga gagtgtacat
     1861 ttatgaatgc aacgattgtg caagaaaata aattgttagc agatggtatt ttgcaatcaa
     1921 gatattttga aaatttgaaa aatagttgga aagatttaga tatagctgta gtcggaattg
     1981 gtgattttag caataaagga aagcatcaat ggttagacat gcttacagag gatgacttta
     2041 aagaattgac caaagttaaa actgtaggag aaatttgttg tcgatttttt gattcaaaag
     2101 gtaaagaagt ctatgaaaac ttacaggaaa gaacgatagc aatctctttg gaagatttaa
     2161 aaaatattcc tcaaagttta gctgttgctt acggtgatac gaaagtatct tcgattcttt
     2221 ctgtcttgcg tgctaattta gtaaatcatt tgattacaga taaaaataca attttaaaag
     2281 ttttggaaga agatggggat ttgactttta gagagattct aggtgagtga aaatgataga
     2341 ctgatcagtt tatcgttttt tctttttagt cgattgcaca tttgtgctca tataaacaaa
     2401 aatagtttat ctgttgtttt tggattgaca actttattat gtagttgtat tctatagtta
     2461 caaaagaaaa ttttaaaatt tcaaatgaaa aaagcttttt acatagtgaa atgaggagga
     2521 atttatggaa attattgttc cagatcaaat tatcatgggt ttaattttat atgctggtga
     2581 tgcgaaacaa catatttata aagcgttaga ttacataaaa aatggtacat gtgaacggtg
     2641 tgaagaagaa atccagttag ctgatgccgc cttattagaa gctcataatc tacaaacaaa
     2701 atttttggca caggaagcgt ctggtacaaa gacagaaatt acagctctct ttgttcattc
     2761 acaagatcat ctcatgacta gtatgacgga gattaattta atcaaagaaa ttattagttt
     2821 gagaaaagaa cttcataaaa aataatacta gagtattatc attgttatta acatagagga
     2881 ggaaaacata atggtgaaga ttggtttgtt ttgtgcagca ggtttttcta ctggtatgct
     2941 tgtaaataat atgaaaattg cagcgcaagc tagtggagtt gaggcagaaa tagaggcgtt
     3001 ttctcagtct aaattagcgg attatgcgcc aaatatagat gttgcactat tgggtccaca
     3061 agttgcttat acattagata aatcaaaaga aatttgtgat aagtgtgatg ttccgatagc
     3121 tgttattccg atgatggact atggtatgtt agatgggaaa aaagtattag atttggccct
     3181 atctttgatt agtgggtaag aaaaggagat ttattatgtc aaagatggat gttcagaaaa
     3241 tcattgcacc gatgatgaag tttgtgaata tgcgtggcat tatagctcta aaagatggga
     3301 tgttagcaat tttgccattg acagtagttg gtagtttgtt cttgattatg ggacaattgc
     3361 cgttcgaagg attaaataag agcattgcta gtgtttttgg agctaattgg acagagccgt
     3421 ttatgcaagt atattcagga acttttgcta ttatgggtct aatttcttgt ttttcaattg
     3481 cctattctta tgctaagaat agcggagtag aggctttacc agctggagtt ctatctgtat
     3541 ctgcattctt tattttgcta agatcatctt atatccctaa acaaggtgag gcgattgggg
     3601 acgctattag taaagtttgg tttggaggcc aaggaattat cggtgctatc attataggtt
     3661 tggtagtagg aagtatttat accttcttta taaagagaaa aattgttatt aagatgccag
     3721 aacaagttcc acaagctatt gccaaacagt ttgaagcaat gattccagca tttgtaattt
     3781 tcttatcttc tatgattgta tatattttag cgaagtcatt gactaatggc ggaacattca
     3841 tagaaatgat ttattctgct attcaagttc cgttgcaagg tttaactgga tctttgtatg
     3901 gtgctattgg aattgcattc tttatatcat ttttgtggtg gtttggtgtt catgggcaat
     3961 cggtagtaaa tggagtagtg acagctctgc ttttatctaa tcttgacgct aataaagcta
     4021 tgttagcctc tgctaatcta tcattagaaa atggtgcaca tattgttact caacaatttt
     4081 tagattcatt tttaattcta tcaggttcag ggattacgtt tggtcttgta gttgccatgc
     4141 tttttgcagc aaaatcaaaa caataccaag ccttaggaaa agttgcagct tttccagcaa
     4201 tatttaacgt aaatgagcca gttgtatttg gatttccgat tgtcatgaat ccagttatgt
     4261 ttgtaccttt cattcttgtt cctgtacttg cagctgtgat agtatatgga gctattgcaa
     4321 caggtttcat gcagccattc tcaggggtaa cattgccttg gagtacacca gctattctat
     4381 caggattttt ggtgggtgga tggcaaggtg ttgtgactca gttattagta ttggtgatat
     4441 ctacagtggt ttactttcca ttctttaaga tacaagatcg tttagcttac caaaatgaaa
     4501 tcaaacaatc ttagaggtat ttgtgtgtta ctgttaaact cacacatttg tgctaaaaat
     4561 tagagagtta aaatttttct agttaaaagc ttgaaaattt ctataaaaat cggtattata
     4621 ttttcgaaag aaataaaaat attttcgaaa gaaaggtgct tacgatggta aatacagaag
     4681 tagcaagaac aacaatcaag acagaatatt ttggcagcct tactgaaagg atgaacaaat
     4741 atcgagaaga tgttttaaat aaaaaacctt atattgatgc tgagagagca gttctagcaa
     4801 cacgcgccta tgaacgatac aaggaacaac ctaatgtcct aaaacgtgca tatatgctga
     4861 aagaaatttt ggaaaatatg tctatctata ttgaagaaga atctatgatt gcgggaaatc
     4921 aagcttcttc caataaagat gctcctattt ttccggaata tacgctagaa tttgttctca
     4981 atgagttgga tctttttgaa aagcgtgatg gagatgtttt ctatattaca gaagaaacaa
     5041 aagaacaact tagaagtatt gctccgtttt gggaaaataa taatttacgt gctagagctg
     5101 gtgccttatt acctgaagaa gtgtcagttt atatggaaac aggattcttc ggtatggaag
     5161 gtaagatgaa ttctggagat gctcacttag cagttaacta tcagaaactt ttgcaatttg
     5221 gtttaagagg ttttgaagag cgggctcgta aagcaaaagt agctctagat ttaacagatc
     5281 cagcaagtat tgataaatat catttttacg actctatatt tatcgtaatc gatgctatta
     5341 aagtatatgc aaagcgcttt gttgctcttg ctaaaagttt agccgaaaat gcaaatccta
     5401 aacgtaagaa agaattactt gagattgcag atatttgctc tagagtccca tatgaaccgg
     5461 caactacttt tgcagaagct attcaatcag tttggtttat tcaatgtatt ttacaaattg
     5521 aatctaatgg ccactctctt tcatatggcc gttttgatca atatatgtat ccatatatga
     5581 aggctgattt agaaagtggt aaagaaacag aagatagcat tgttgaacgt ctgacaaatc
     5641 tttggattaa gacaattaca attaataagg ttcgcagtca atcacataca ttttcttcag
     5701 caggaagtcc tttatatcaa aatgttacaa ttggtggaca gactcgagat aagaaggatg
     5761 ctgttaaccc attatcttat ttggtattaa aatcagttgc acaaacccat ctaccgcaac
     5821 ctaatctaac tgtacgttac catgcaggtt tagatgctcg tttcatgaat gagtgtattg
     5881 aagtgatgaa acttggtttt ggtatgcctg catttaataa tgatgagatt attattcctt
     5941 cttttattgc aaaaggagta ttggaagatg atgcttatga ttacagtgcc attggatgtg
     6001 ttgaaacggc agttccaggg aaatggggct atcgttgcac aggtatgagt tatatgaact
     6061 tccctaaggt tctacttatc acgatgaatg atggaattga tccggcttcg ggtaaacggt
     6121 ttgcaccaag ctttggtcat tttaaggata tgaagaactt ttctgaatta gaaaatgctt
     6181 gggataaaac actaagatat ttgacacgaa tgagtgttat tgttgaaaat tctattgatt
     6241 tatcattgga acgagaagtt cctgatattc tatgttcagc attgactgat gattgtattg
     6301 gtcgtggaaa acaccttaaa gaaggtggag cagtatatga ttatatatca ggattgcaag
     6361 ttggaattgc aaatttgtcg gattcattag ctgcaattaa aaaattggtg tttgaggaag
     6421 aacgtataag cccaagtcag ctttggcatg cactggaaac agattatgcc ggagaagaag
     6481 gtaaggtcat tcaagaaatg ttgattcatg atgcacctaa gtatggtaat gatgatgatt
     6541 atgctgacaa attggttact gctgcttatg acatttatgt tgatgaaatt gctaaatatc
     6601 ctaatacacg ttatggaaga ggtccaattg gaggaattcg ttattcagga acatcttcta
     6661 tctcagccaa cgtagggcag ggacgtggaa cattagcaac tccagatgga cgcaacgcgg
     6721 gtacaccgtt agcagagggt tgttcaccat cacataatat ggatcaacac ggccctacat
     6781 ctgttttaaa atctgtttca aaattaccaa cagatgaaat cgtaggtggg gttctcttaa
     6841 atcagaaagt aaatcctcaa acgttagcca aagaagaaga taaattaaaa ctaattgctt
     6901 tgttacgaac attctttaat cgtttacatg ggtaccatat tcaatacaat gttgtttcca
     6961 gagagacgct gattgacgct cagaaacatc ctgaaaaaca cagagactta attgttcgtg
     7021 ttgcaggata ctctgcattc ttcaatgttc tttctaaggc aacccaagat gacattatag
     7081 gacgtactga gcatactttg taaaataaag aggttctttt tatggaattt atgcttgaca
     7141 cattaaattt agatgagatt aaaaagtggt ctgaaatttt gccgctagct ggggtaactt
     7201 caaatcccac tattgcaaaa agagagggtt ctattaattt ttttgaacga atcaaagatg
     7261 taagagaatt gattggctct acaccctcta ttcatgttca ggtgatttct caagattttg
     7321 aaggcatctt aaaggatgct catgaaattc gaagacaagc aggagatgat atatttatca
     7381 aagtacctgt tactccagct ggattacgtg caataaaggt gctaaaaaaa gagggctacc
     7441 atatcactgc aacagctatt tatacagtta ttcagggatt attagctatc gaagcaggag
     7501 ctgattacct agctccatat tataatagaa tggaaaatct gaacattgat tcaaattctg
     7561 tcattcgtca attagctctt gctattgata gacagaactc tcctagtaag attttagctg
     7621 catcctttaa aaatgtagca caagtaaata atgctttagc tgcaggtgcg catgctgtta
     7681 cagcaggagc agatgttttt gaatcagctt tcgccatgcc atctatccaa aaggcggttg
     7741 atgatttttc tgacgattgg tttgttactc aaaatagtcg ttccatttag atagagagga
     7801 aatatatatg agaatttttg ctagtccttc tagatatatt cagggggaaa atgccttgtt
     7861 tgaaaatgcc aaatcaattt tggatttggg aaattaccct attctattat gcgatcagtt
     7921 ggtttatgat attgttggaa aacgatttga agattaccta cataggtatg gtttccatat
     7981 tgttctggcg ctatttaatg gtgaagcttc tgacaatgaa atcaatcgag ttgttgcctt
     8041 ggctgagaaa gaaaattgtg atagtattat cggtcttggt gggggaaaga cgattgatag
     8101 cgcaaaagct attgcagatt tgattgaaaa gcctgttatt attgctccaa caattgcatc
     8161 gaccgacgca cctgtatctg ctttatctgt tatttataca gatgaaggtg catttgatca
     8221 ttatctattt tattctaaaa atccagattt agttttggtt gatacaaaag ttatttcaca
     8281 agcccctaag cgtttattag cgtctggtat tgcagatggt ttagcaactt gggttgaggc
     8341 gcgtgcggtt atgcaggcaa atggaaaaac tatgttggga caacagcaaa cattggctgg
     8401 agttgcaatt gcgaagaaat gtgaagaaac gctgtttgca gatggtttac aggctatggc
     8461 agcttgtgaa gctaaagtgg tgacaccagc attagaaaat attgttgaag ctaatacttt
     8521 attgagtggt ctaggttttg aaagtggagg attagctgcg gcgcatgcaa ttcataatgg
     8581 ttttactgca ttgacaggtg acattcatca tttaacacat ggtgaaaaag tagcttatgg
     8641 aactttagta caactattat tggaaaatag acctaaagaa gaacttgata agtatattga
     8701 gttttacaaa aaaattggta tgccaacaac tctaaaagaa atgcatttgg atcaagttgg
     8761 atatgatgat ttaataaaag ttggtaaaca agcaactatg gagggtgaga caattcatca
     8821 gatgccgttt aagatttcgc cttcagatgt tgctcaagct attatcgctg tagatgccta
     8881 tgtaaattca aaataaacaa taaggactac tgttttccaa atggtagtct tttattgatc
     8941 cccgtattga attctataga agattgaaat aggatgag
//
LOCUS       DAVKZB010000075         8798 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKZB010000075 DAVKZB010000000
VERSION     DAVKZB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.075"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..131
                     /locus_tag="VXA80_001496"
     CDS             <1..131
                     /locus_tag="VXA80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV0353973.1"
                     /translation="IKTSSDIELESDVDGDKSDNLPVDIKILGQHIEVYSVPKKQS"
     gene            331..3606
                     /gene="rexB"
                     /locus_tag="VXA80_001497"
     CDS             331..3606
                     /gene="rexB"
                     /locus_tag="VXA80_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV0353974.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLATYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3603..7253
                     /gene="addA"
                     /locus_tag="VXA80_001498"
     CDS             3603..7253
                     /gene="addA"
                     /locus_tag="VXA80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV0353975.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSAQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDTKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQVYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKIQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVQENLYEKLVNTQKQVS
                     SQKVLIHSTLAEKLNQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIGMINQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMDTGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNIRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7513..8691
                     /locus_tag="VXA80_001499"
     CDS             7513..8691
                     /locus_tag="VXA80_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353976.1"
                     /translation="MTLPVRKSLHDAVLQASKANTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2715 a         1848 c         1867 g         2368 t
ORIGIN      
        1 atattaaaac ttcttctgat atcgaattag agtccgatgt tgatggcgat aaatcagaca
       61 atctacccgt cgacattaag atactgggac aacacattga agtgtattcc gtacctaaga
      121 aacaatcata aaactcaaag tcttgttacc gaggcttttt tattatgtga ttttcagtag
      181 ccagacttat tctatctaat acaagtaaag ctaagtattt aagatttagc agatgatgaa
      241 gaaaagaaag gatggcctaa atattttaac agatttacga ggctaatcat ggtataatat
      301 agggtgatga aaaattccaa cgaggctgag atgaaattac tttatactga tattcgaact
      361 tctttgacag aaattctaac aagagaggca gaagagctag ttgcagctgg caagcgggtc
      421 ttctacattg cccccaactc tctttctttt gaaaaggaac gcgccgtgct ggaatgcttg
      481 tcccagcagg cttctttttc gattaccgtc acgcgctttg ctcaaatggc tcgttatctg
      541 gtcttgaatg atttaccagt taaaactact cttgatgata tcggtcttgg gttggccttt
      601 tacaaatgcc ttgccgaact cgatcccaag gacttgcgtg tttatggcgc tattaagcag
      661 gatcctcaat tgatccagca gttaattgag ctttaccatg agatgaccaa atctcagatg
      721 agttttttgg acttggagaa tttaacagat gaggataaga ggacggattt actcttgatt
      781 tttgagaaag taacagccta tcttaatcaa ggtcagttag cccagggaag tcagttgtcc
      841 catttgattg aggctattga gaatgacaag gtaagtagtg attttaatca aatcaccttg
      901 gtcattgacg gctttactcg tttttctgct gaggaagagc gggttgtgga cttacttcac
      961 ggcaaaggtg ttgagattgt tatcggggct tatgctagta agaaagccta taccagtcct
     1021 tttagcgagg gcaatctcta ccaagccagc gtaaaatttc tccatcatct ggcttctaaa
     1081 taccaaacgc ctgctcagga ctgttctcaa actcatgaga agatggatag ttttgacaag
     1141 gcctctcgtt tgttggagtc ttcttatgac ttttcagaac tcgctttgga tgtcgatgag
     1201 aaagaccgtg aaaatttaca aatctggtct tgtttgacgc aaaaggagga gttggagcta
     1261 gtagcccgta gcattcgtca gaaattacat gagaactcag acctgagcta caagcatttt
     1321 cgtattctct tgggggatgt agcttcttac cagttatctc tcaaaaccat ttttgaccag
     1381 tatcagattc ctttttatct tggtagaagc gaagccatgg ctcatcatcc cttgactcag
     1441 tttgtcgagt ctattttagc tttaaaacgt taccgttttc gtcaggagga tttgattaat
     1501 cttcttagaa ctgatttgta tactgacctc agtcagtctg atattgatgc ttttgagcaa
     1561 tatatccgct atcttggtat caatggcttg ccagcctttc agcaaacctt caccaaatcc
     1621 caccatggaa aatttaatct tgagcgtttg aatgtcctcc gcctgagaat tttagcacct
     1681 cttgaaaccc tctttgccag ccgaaaacaa aaggctgaaa acctcctaca aaaatggagt
     1741 gtctttctaa aagaaggagc tgtgaccaag cagttacaag atttgacaac cactttggaa
     1801 gctgtagaac aggaaagaca agccgaagtt tggaaggctt tctgccatgt tttagaacaa
     1861 tttgcgactg tttttgctgg ttcacaggtt agtctggaag acttcctagc cttgctccat
     1921 tctggaatga gtttgtccca ataccgtacc attccagcaa cagtggacac tgttctggtg
     1981 cagagttacg atttgattgc accattgact gctgactttg tctatgctat tggactaact
     2041 caggacaatt taccaaaaat ttctcaaaac accagtcttc tgacagatga agaaaggcaa
     2101 aacctaaacc aaacgaccga agaaggcgtt caattactga ttgccagcag tgaaaatctc
     2161 aagaaaaatc gctacactat gctttccttg gtcaattctg ctcgtaagca gttgttcttg
     2221 tcggctccaa gcctttttaa cgaaagtgaa agtaaggaat ctgcctatct tcaagagttg
     2281 atccattttg gatttaggcg gagagagaag aggatgaatc acaaaggact gtctaaggag
     2341 gatatggggt cctatcacag tcttttgtct agtctggttg cctatcacca gcagggtgag
     2401 atgagcgata ctgagcaaga tttgactttt gtcaaggttc tgtcgcgtgt cataggtaaa
     2461 aaactagatc tgcaaggtct ggaaaatcca gctatcccaa ccagtccaag cagcaagacc
     2521 ttagccaagg acaccttgca agctctctat cctgccaaac aggagtttta cctgtctacg
     2581 tcgggtttga cagagtttta tcgcaatgaa tacagttatt tcctacgcta cgttttaggc
     2641 ttgcaggagg aattacgttt gcgtcctgat gcccgtagtc acgggaattt cttgcatcgt
     2701 atctttgaac gcgccttaca gttgcctaat gaagattcct ttgaccaacg tctagaacaa
     2761 gctattcaag aaaccagtca agaacgcgaa tttgaagcta tttatcaaga aagtttggaa
     2821 gcccagttta ccaaggaagt tttgcttgat gttgcacgga cgactggaca tattctccga
     2881 cacaatccag ccatcgaaac catcaaagaa gaagcaaatt ttggtggaaa agaccaagcc
     2941 tttattcaat tagacaatgg acgcagtgtc tttgtacgag gcaaggtgga ccggattgac
     3001 cgtttgaaag ctaatggagc gataggagta gtagactaca aatccagtct gactcagttc
     3061 cagtttcctc atttctttaa tgggctcaat tctcagttag caacctatct tgctgcccta
     3121 aaaagagaag gggagcagaa ctttttcggc gccatgtact tggaaatggc tgaacctgtc
     3181 caatctctga tggcggtaaa aagtctggca ggagcagtgg tagaagccag caaatctatg
     3241 aaataccaag ggctcttctt ggaaaaagaa agcagttatt taggcgaatt ttataacaaa
     3301 aacaaggcta atcaactgac agatgaggaa tttcagctcc tactggacta caatgcctat
     3361 ctttacaaga aagctgctga gaagatttta gcaggccggt tcgccatcaa tccttatact
     3421 gaaaatggca gaagcattgc cccatacgtc cagcaacatc aggctattac aggctttgaa
     3481 gccaattacc atctgggcca agcccgtttc ctagaaaagt tggacctagc tgatggcaag
     3541 cgtctggtcg gagaaaaact caagcaagct tggcttgaaa aaataagaga ggagttgaat
     3601 cgatgaagct tattcccttt ttaagtgagg aggagattca aaaactgcaa gaagcagaag
     3661 caaattcgag caaggaacag aagaaaactg ccgagcaaat cgaagctatc tacacttctg
     3721 cccagaatat cctggtctca gcatcggctg gttctggaaa gacctttgtc atggcagagc
     3781 gcattctgga ccaattggcg cgtggtgtcg aaatttctca actctttatc tcaaccttta
     3841 ccgtcaaggc tgcaactgaa cttaaagaac gtttagagaa aaaaatcagc aagaaaatcc
     3901 aagaaacaga tgatgtcgac ctcaaacaac acttgggtcg ccagttggca gacctaccca
     3961 acgctgccat tggaaccatg gattctttca cacaaaaatt ccttggcaaa catggttatc
     4021 tgcttgatat tgcacctaat ttccgtattt tacaaaacca aagcgagcaa cttcttctcg
     4081 aaaacgaagt ctttcatgag gtctttgaag cgcattacca aggtaaacag aaagagacct
     4141 ttagtcattt gctgaaaaac tttgctgggc gtggcaagga cgaacggggt ctgcgccagc
     4201 aggtctataa aatctatgac ttcctccaat ccaccagtaa tcctcaaaaa tggctgagtg
     4261 aatctttcct caaaggattt gagaaagctg attttaccag tgaaaaagaa aaactgactg
     4321 agcaaatcaa acaagccctt tgggatttgg aaagcttttt ccgttaccat ctggataacg
     4381 ataccaagga gtttgcaaag gctgcctatt tagaaaatgt tcagttaatt ctggatgaaa
     4441 ttggctccct aaatcaggag tccgatagtc aggtttatca ggcagtgctt gcgcgtgttg
     4501 tcgccatctc taaggagaaa aacggtcgag ctctgactaa tgccagccgt aaggctgatt
     4561 tgaagcccct ggctgatgcc tacaacgaag agagaaagat ccagtttact aaactaggac
     4621 aattatcaga ccagatagcg attctcgact atcaagaacg ttatcatgga gacacttgga
     4681 aactagctaa aaccttccaa tctttcatga gcgattttgt agaggcttat cgtcagagaa
     4741 aacgacagga aaatgccttt gaattcgctg atatcagcca ttacaccatt gagattttag
     4801 agaatttccc acaagttcgt gagtcttatc aggagcgctt ccatgaagtc atggtcgatg
     4861 agtatcagga taccaaccat attcaagaac gaatgctgga attgttgtct aatggccaca
     4921 atcgctttat ggtgggagat atcaagcaat ccatctatcg tttccgtcag gcagacccgc
     4981 agattttcaa tgagaaattc caacgctatg cgcaaaatcc ccaagaaggc aagctcattc
     5041 tcctcaagga aaatttccgt agtagttcag aagtgctgtc agcaaccaat gatgtctttg
     5101 aacgtctcat ggaccaagag gtcggcgaaa tcaactatga taacaagcac cagcttgttt
     5161 ttgccaatac caaactgact cccaatccag acaacaaggc agaatttctc ctctacgaca
     5221 aggacgatac aggtgaggaa gaagagagtc aaacagaaac gaaactaaca ggcgaaatgc
     5281 gcttagttat caaggagatt ctgaaacttc atcaagaaaa aggtgttgcc tttaaggaaa
     5341 ttgcccttct gacctccagc cgcagtcgta atgaccagat tctcctcgcc ctgtctgagt
     5401 acggaattcc tgtcaaaact gacggtgaac aaaacaatta tctccaatcc ctagaagtgc
     5461 aagtcatgct agataccctt cgtgtcattc acaatcccct gcaagactac gccttggttg
     5521 ctcttatgaa gtctccaatg tttggttttg atgaggacga gttagcacgt ttgtctcttc
     5581 agaaagcaga ggataaagtc caagaaaatc tttatgagaa actagtcaat actcaaaaac
     5641 aggtaagtag ccaaaaagtc ttgattcact caactctagc tgagaaacta aaccaattca
     5701 tggatatatt ggcttcttgg cgcctgtatg ccaaaaccca ctccctctat gacttgattt
     5761 ggaagattta caacgaccgt ttttactacg attatgttgg agctttgcca aatggtcctg
     5821 ctagacaggc caatctctat gccctagcac tgcgtgctga tcaatttgaa aagagcaatt
     5881 tcaagggctt gtcgcgtttt attggtatga ttaaccaagt tttagaagcc cagcacgatt
     5941 tggcaagtgt tgcagttgca ccacctaagg acgcagtaga gcttatgacc atccacaaga
     6001 gtaaagggct ggagtttcct tatgtcttta tcctcaatat ggatcaagat ttcaacaagc
     6061 aagactctat gtcagaggtc attctcagcc gtcagaatgg tcttggtgtc aagtatattg
     6121 ccaagatgga tacaggagca gtggaagatc actatcctaa aaccatcaaa ctctccattc
     6181 ctagcctgac ctataggcag aacgaagagg aattacagct agcaagctat tctgagcaga
     6241 tgcgtttgct gtatgttgct atgacgcggg ctgagaaaaa gctctatctt gtcggcaagg
     6301 gttctcgtga aaagctggaa tccaaggaat acccagcagc caaaaatggg aaactaaata
     6361 gcaatattag actgcaagca cggaatttcc aagattggct ttgggctatc agtaaagtgt
     6421 ttactaagga caagctcaac tttagttatc gttttattgg cgaagatcag ttgaccagag
     6481 aagctatcgg agagttggaa accaagagtc ctctccaaga tagctcccaa gcagacaatc
     6541 gtcagtcaga taccatcaaa gaagctctgg aaatgctgaa ggaggtggaa gtttataata
     6601 ctcttcaccg cgcagctatt gaacttccta gtgttcaaac tccaagtcaa atcaagaaat
     6661 tctacgaacc agttatggat atggaaggtg tcgagattgc tggtcaaggt cagtcagtag
     6721 gcaagaaaat cagctttgat ttgccagatt tttcaaccaa agaaaaggta actggagctg
     6781 agattggtag tgctactcac gaactcatgc agagaattga cctcagccag caactaaccc
     6841 ttgctagcct aacagaaaca ctcaaacaag ttcaaactag ccaagctgtc agagacaaga
     6901 tcaatcttga taaaattctt gctttctttg acacagcact cggtcaggaa attcttgcta
     6961 ataccgacca tctctatcgc gagcaacctt tctccatgct caaacgagac caaaagagtc
     7021 aggaagactt tgttgtccgt ggtatccttg atggctatct gctttacgaa aacaaaattg
     7081 ttctgttcga ctacaagaca gaccgctatg atgaaccaag tcaactcgta gaccgctatc
     7141 gtggtcagtt agctctatac gaagaggctt tatcacgagc ctattcgatt gaaaatattg
     7201 aaaaatactt gattttactc ggtaaagacg aggttcaagt tgtaaaagta tagcttatac
     7261 tcttcgaaaa tctcttcaaa ccacgtcagc ttcgccttgc tgtatatatg gttactgact
     7321 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgacttcgtc agttctatct
     7381 acaacctcaa aacagtgttt tgagctgact tcgtcagttc tatctacaac ctcaaaacag
     7441 tgttttgagc tgactgcggc tagctttcta gtttgctctt tgatttttat tgagtattag
     7501 aaaggagacc tcatgacact tccagttaga aaatccctgc acgatgcagt tttacaggct
     7561 tcgaaagcga atacttggga acaagctacc aaggaatgga atgaagtttc cttgattttt
     7621 aacggtatcg gccgtagcaa ttgtgtctgt ggaaatgcca tcaaatacgc ttatgaactc
     7681 tttaacggtg tcacaggcca acgcctcttt ccaattggta gcgactgtgt tcgtcatttt
     7741 catcgattgt cccttgacca gcaattggaa gaggaagaaa aactgctcag aaaggttgaa
     7801 aatctaacca gaaaagctca gaaaaaggaa aaaatcaagg tcaataaaag tgactttgac
     7861 gagcgacttc taaaatggct ctgggaaaaa ggtgttttca aacccaatcg tggcaatcaa
     7921 tttgcgcctg agagagacta ccagcttttc ctagaagtct ttcagggagg aagttggacc
     7981 aaggcggaac caaagaaaaa ggctcggatg gaagaagtcc ttgaaaagtg tatcaaaccc
     8041 tttttacttg ggaaatcgga tgaccaactc taccttgtca agctaggcaa ggagaaaatt
     8101 gactacgaac aagaactccg tatccaggca gagaaagaac gcaagaagag ggataaaatc
     8161 gccaagcaat atgcagacaa tctggttcta gccatgggac ccgcagagcg tgcttatcaa
     8221 gattactttg gctttacaga gaccctgact caagaagaac gcaagtggga aaaaattctt
     8281 tttggtaaaa atagagcaga acgagctatc aaggccaaac aataccaaaa agagctggaa
     8341 aaagaccaac gaattgccag tcaggatcca attgaaagaa aacagaagca gacttggatc
     8401 ctcaattcct attttcgtga gcttcctgaa gaaaaagctc gattttctag actcttatta
     8461 gaatatcgaa aaagtggtga agtacccttt tcaactgaat acctgtcaga tcatctcatc
     8521 gactttttct ataaaatgaa agcctttgaa tttgaaatcg caccagaaca agtccgagat
     8581 tttctaaaaa aaagcctgca ggaagaccat cgctcatcag cacaaggaag ctggatagaa
     8641 ggaattctcc tcaactgcct caaaccattt ttagaacgat tagtgatatg agtaacacct
     8701 accgtgtaaa tttcatggta ggtgttattg tacccccaaa taatataaaa caaagtatga
     8761 aagcgcatct caattttaag acttttttgt aaattaag
//
LOCUS       DAVKZB010000076         8657 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKZB010000076 DAVKZB010000000
VERSION     DAVKZB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.076"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            217..1092
                     /locus_tag="VXA80_001500"
     CDS             217..1092
                     /locus_tag="VXA80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0353977.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPDKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHP
                     YASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWM
                     ANLNLKFSVRNKSRN"
     gene            1118..1762
                     /locus_tag="VXA80_001501"
     CDS             1118..1762
                     /locus_tag="VXA80_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV0353978.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1804..3792)
                     /locus_tag="VXA80_001502"
     CDS             complement(1804..3792)
                     /locus_tag="VXA80_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0353979.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3794..4552)
                     /locus_tag="VXA80_001503"
     CDS             complement(3794..4552)
                     /locus_tag="VXA80_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353980.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4980..5171
                     /locus_tag="VXA80_001504"
     CDS             4980..5171
                     /locus_tag="VXA80_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353981.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5525..5797)
                     /locus_tag="VXA80_001505"
     CDS             complement(5525..5797)
                     /locus_tag="VXA80_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0353982.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5856..6305)
                     /locus_tag="VXA80_001506"
     CDS             complement(5856..6305)
                     /locus_tag="VXA80_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV0353983.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            complement(6292..8421)
                     /locus_tag="VXA80_001507"
     CDS             complement(6292..8421)
                     /locus_tag="VXA80_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV0353984.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRSTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DINDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2637 a         1806 c         1670 g         2544 t
ORIGIN      
        1 aaacattatt agaaatagat tttactgtcc tgatcgattt gttctgttct tgtttcattt
       61 tactatatgt tcgtaaatga aagttctata atgaagttag ccacccagcc ctcctaaaaa
      121 ttagatggtt caactagctg atagctcttg taggcataag aaaacacctc tgtgttatac
      181 ttgttgttca accacaaaca caagaaaggc acagaaatgc aagacaatta tactacaaaa
      241 ggtaaacatt tgacaatcga tagccgtcgc ttaatcgaaa gatggaaaaa agaaggaaaa
      301 tcaaatagag aaattgcctc tctacttgga aaagctcctc aaactatcca cactgaaatc
      361 aagcgtggga cagtccgaca atgtcttgga aaagggcgct tcaaagaggt ttattctgcc
      421 gactatgctc aacagtctta tgaaaacaat cgcaagcgct cggtcaagaa atcaagcttg
      481 accaaggaac taaaggaaaa gattctccac tatcataacc aaaaattttc gcctgataag
      541 aaacaggcta gcaccaactt taaacctgct ggtcaatcca tcgaacagcg gtctgaagct
      601 atcaatcttc gcttggagaa tgggcattat gagattgata cggttctact tacgagagcg
      661 aaaaactact gcttgcttgt cttgacggat cgaaagagta gacatcagat catccgattg
      721 attccaaata aaagtgctga ggtggtcaat caggctctaa aactcatctt aaaacaacac
      781 aagattcttt ccatcacggc agataatgga acggaattca atcgcttgtt tgatgtattt
      841 tctgaggagc acatctatta tgcgcacccc tatgcctctt gggaaagggg aactaatgag
      901 aatcacaaca ggctcattcg tagatggtta cctaagggaa ccaagaaaat gactcccaaa
      961 gaagtcgcat tcatcgaaaa gtggattaac aactatccta aaaaatgctt ggactacaag
     1021 tcacccagag aagacttctg gatggctaac ttgaacttga aatttagcgt cagaaacaaa
     1081 tcacgaaatt gattcaaatt ttgcaggtcg tttaaatatc ctgcgtgcgg gtgttcttga
     1141 tgctaacgat ggaattattt ccattgctgg tgtggttatc ggagttgcca gtgccacgac
     1201 caatatctgg attatctttt tatcaggttt tacggctatc ttagctggtg ccttttcaat
     1261 ggctggtgga gaatatgtat ccgtttcaac tccaaaagat accgaggaag ctgccgtttc
     1321 gcgagaaaaa ctcttgctag accaagatag ggaactagcc aaaaaatccc tctatgctgc
     1381 ttatatccaa aatggagaat tcaaaacttc tgcccaactc ttgaccaata agatctttct
     1441 taaaaatcca ctcaaggctc tggtagagga aaaatatggg attgagtatg aagaatttac
     1501 caatccttgg cacgctgcca tttctagctt cgttgccttt ttccttagaa gtttgcctcc
     1561 aatgctgtca gtgaccattt ttccaagtga ttaccgcatc cctgctaccg tccttattgt
     1621 cggtgtggcc cttcttctca ctggttacac aagtgctaga cttggaaaag ccccaaccaa
     1681 aacagctatg attcggaacc ttgctattgg tctcttgacc atgggcgtta ccttcctgct
     1741 cgaacaactt ttcagcattt agaatacaag aaatacctcg attttgaagt cgaggtatct
     1801 tttttacatt tgcacaatct tgcgataact tcttgaagta atcatgaaaa tcagcacata
     1861 ggcgatgagg aagatagcgc agatagacaa ggtcacaatc aacatcatag tcgtatccag
     1921 tacaccaatc acttttaaaa tcaggctaag catatggtag gcaaaggcga gatgtatgaa
     1981 ggcaaagagc aaaggaagga agaaaacagt taaaacctgt ttgttgatgg tttgcttgat
     2041 ttgcttttgg tccaaaccga ctttctgcaa gataataaag cgttcacggt cttcgtagcc
     2101 ttcagaaatt tgtttgtagt agatgaccag aacagttccg accataaaga taatggatag
     2161 gaaaataccg ataaagaaga caccaccaaa gagggcactc atctgagaac tagcatctgc
     2221 tagattgcta ccataaacat agctaccttc tgtgtctaat tgagcattaa attgattgag
     2281 gtagttttca tactcctcag cgaccttgag ttgttcttct tcgctggcat ctacattcat
     2341 accaccgtaa aactgattat agatagccga atctgggaat tgatccaaaa aggcttgtaa
     2401 atcaggaaca acaaggtaat tgtaatcagt agtcaagata ttaaacttat ttggaacatg
     2461 gttgacaatg aaatctttat taaattcttc tttgacagaa aattgatgat catttagagt
     2521 tagagctttc tgtcctttca gtccgtcatt tttggcaaag agaccgacct catttcctga
     2581 tagagacagt ttttgaccag tcatattttc ataatctttt tggtcaaata ccatgaaaac
     2641 tgtttttggt tggacacggt tttgtccttt ttcaaaaata gttaacttgg ttccttcttg
     2701 attcgcaaca gcaaagtaag tgtaacgaaa tacttctttt tctttaatct tataaccttt
     2761 gtcacttgca aactggctca agagtttgtc caagtcttct ttttcaacat tttgccctga
     2821 aaccccaaaa tcatgaggat ttagaacttt tttaaagctt tctgaggaat tgaaaatgct
     2881 tgtcgctgct gacatggtta ccaaaaccat tgttgacaag atagcgatgg tggctagtcc
     2941 aactgcattt ttcttcatac ggaaaatcaa gttagaaaca gatatgagat tatttggttg
     3001 gtaatagtat ttcttatttt tctttaagat ttggaggaaa acggtaatcc ctgcattaaa
     3061 caagagataa gtcccaaaga taaccagtaa aacagctatg aagaaggttg ttaaggctgt
     3121 aagaggatct tttaccgtaa gggcaagata atagccaatt cctaaactta tggaaccaag
     3181 aatggtttgg agaggaagga agcgaccttt tttctctcca ctagctttct cacgagagag
     3241 ctggagggca ttcatacggg cgattcgaag ggcattcagg aacatgagac ctaggaaaat
     3301 caaaccgaag acaacaagca ctgtaatgac aactttcgtc tggaaggtag caaccagctc
     3361 aaccttcaat ttcattagtt tgagcaggaa agcgaaaatt aacttgtcaa acaaggctcc
     3421 aataccgata cccgctccaa cagttagaat cccaaatacc actaactcct taaaggtcat
     3481 actgataaga tgacgcttct ccaagcccaa catgccataa attcctagtt ccttggaacg
     3541 gttcttcatg acaaaactat tggcatagag aacgataatg gctgacgcaa gggtgacgac
     3601 aaacatacca aatccaagtg tagcctgaat ggttgttcct ccacggattt ccgcaatctt
     3661 aggattgaag gttagggagt aaaagagata ggtgacagtg actgccaaga gaacagccag
     3721 cgcaaaagga tagtagagtt tgcggttttt aatcaagttc gataccgcta acttattggt
     3781 taatcgaaac atactaattc acctcgcttg ccatgacagt caaggtatca gagatttctt
     3841 ggaacatctg acgttctgtc ttctctccac ggtagatttg attgtaaaga atgccgtctt
     3901 tgataaagag tacacgttta gctctgctag ctgctactgt tgagtgggtt accatgagaa
     3961 tggtttggcc gcgctcattg atttcatcaa agacatcaag taaggctgca gatgacttgg
     4021 agtcaagagc tcctgtcggt tcgtccgcaa ggagaatttc aggttcagtg atgatggcgc
     4081 gtgctactgc tacacgctgt ttctggccac cagaaatctc gtaagggtac ttctcttgta
     4141 attggtttat tcccagattc tcagctgtca ccaccaattt cttcatcatc tctgtaatag
     4201 gtcttcttga caagacaagc ggaagcaaga tattgtcctt tacagataga gtatctagca
     4261 agttaaagtc ttggaagaca aagcccaact tttcacgacg gaagctagag gcctgtgaat
     4321 ttttaatggt tgcggtgtca gttccattca agtaaacctg accacgactt ggtttatcca
     4381 gcatagctag aatattgaga agagttgatt taccagaacc agactcaccc atgatggcaa
     4441 cgtagtcacc cttttcgacg gtaaagtgaa tatccttgag ggcttctact tggttgccct
     4501 gaaaacgtgt tttataaatt ttttgaacgt gttttacatc taaaagtgtc atgagaatct
     4561 cctttcttaa tatcccatca aatgaaatgt agcttgcatc atagtgcatc ccagctgaac
     4621 gcgctcaatt tctttgtgac ttggttttct agttttctta tgtaagattt gtttcatgat
     4681 gttactcctt tgttctttat gagtctagtt tacatcaaaa aaagaccgct tgccataacc
     4741 taaccttaca aaagagactt taatcttaca tttttgtaag attgggtgaa atataagcaa
     4801 aattacttag aaaatgattt gatttcctat ttttaaagtt gtaaaatata agtgtgaaaa
     4861 ggcttacttt gaaaaggagg aagcgggaac taacatttta gtaaggttac ctagataaca
     4921 taccattcaa tttaacactt gcaagtcgtt caacctataa ggaaagaagg tgcgctttaa
     4981 tgaaacacaa agaacatata ctcatcggac ttctctatct gctctctcca ttcatcggtc
     5041 agctcttagt tgaacacaca cactttatcg gtacagaatt taccggcact gcttatgtta
     5101 tttgctggtt atcagttgtt atcagcatcc accatttttc caaaaatgta ttgtctcagc
     5161 agcaaaaata gattaaaaaa ctgcgtttaa attactgctt ttatagtaaa atgaaataag
     5221 aacaggacag atcgatcagg atagtcaaat cgatttctaa caatttttta taagtatagg
     5281 tgtcctattc tagttccaat ccactatagt ttgtggatga agctggcgaa atattattaa
     5341 tactattcaa aaatcaaagt gcaaactata tcaacttcac attgccgact aaattacagc
     5401 ctgcggctgg cttcctagtt tgcactttga tttttattga gtataaaagt aaaaaagctt
     5461 taaatcaagc agtttagact attgatttaa agctttttgc gtattccaaa tcttttgtaa
     5521 aacctcacca aaaccagcct tcattatcgt caatggcttg agcttctttg cgtttacgtg
     5581 ggcctgttcc gtacatttct gcttcgattt cttccttggt tggcatgata gaagctagga
     5641 tgatatagat aatcacccca agtccaaagt ttgcgactgt aaaaatggca aacagaaaac
     5701 gtacaagggt aacatcaaag ttccacttgt ctgacagacc tgccaaaact cctgaaatca
     5761 tgcgatttcg tctcatttta taaaatttac tgttcatgat atttcctctt tctgctaggt
     5821 tagacatagt atatcacggg taggctcggc tttcatcagt cctcaggctg atttattagt
     5881 agcaatttac ctcgacaaag tccacagcga tagcgtctgg tatcaattct tcgcttgcgc
     5941 tgataagttt gctggcagga ttggcaacga tagagcttga ttggcttgga gttactattg
     6001 ggcaaggatg gtacaaaccg taatccatcc actgctttca acagttcctt aaaatcccga
     6061 tccttgtgtt gatagccttt cccttgaaaa tagaggtgat aatgacagag ttcatgtcgg
     6121 acaattttcc taaaaacgtc caaccccagt tcctgataaa ccttgggatt aaaatccaaa
     6181 tgcccatctt tggggaaaaa tcgcccacct gtcgaacgta gacgcctatt ccactggaca
     6241 tgatggataa aaggtctgcc gaagtcttct agtgaaacct gcttgacgta atcagtcagt
     6301 ttcatttgga gctaggagag acagattaac tttttcacgt tcagtatcaa ttttcttaac
     6361 ccaaacgctc accaaatctc caactgccac cacttgacta gggtgtttga taaacttgcg
     6421 actcatatgg gaaatatgga tgagaccgtc ctcatgaatt ccgatatcaa caaaagcacc
     6481 gaaatcaaca acgttacgca ccactccttc tagcttttgt ccaaccacta agtccttgat
     6541 atctaggaca tcttgacgga gaactggagc atcaaaggaa tcacggaaat ctcgacctgg
     6601 tttgagaaga tctgcaatga tatctttaag agtttctgga ccaaggtcta actcttgcgc
     6661 catttccttg actgaaagcg acttgagttt gctttgggcc tcttcattca gatcattaat
     6721 gtccaagcat ttgaagagtt ccttaacggc agcgtaattt tctgggtgaa ctcctgtatt
     6781 atcaaggata ttgctacttt cagggatacg gaggaaacca gcagcctgtt caaaagcctt
     6841 ggcccccaaa cgaggaactt tcttgatttg ggagcgtgaa gtgatttttc cctcttcctc
     6901 acggtatttg acgatatttt cagaaatggt tttattgagt ccagctacgt gtgaaagaag
     6961 agctgggcta gctgtattga cattgacacc aacttggtta accactgtat cgacaacaaa
     7021 gtccagactc tcagatagtt tcttctgact gacatcgtgt tggtattgac cgacaccaat
     7081 tgacttagga tcgattttga ccaattccgc aagaggatct tgcaaacgac gggcgataga
     7141 aatggcagag cgtttttcaa cggtcaagtc tggaaactcc tgacgagcaa gttcgctggc
     7201 agaatagaca gaagcaccac tttcattaac gataacatag ctgacttcag ggaaatcttt
     7261 cagaacttcc gctacaaaag cttcactttc acgactggcc gttccatttc caatggcaat
     7321 aatctctaca ccgtattgac caattaaatc tgctaaatct ttcttggctt cttcgatttg
     7381 acgagctgat gctggtttaa caggataaat aacctgagtt gtcagcattt ttcctgttgc
     7441 atccacgaca gctagcttgg cacctgtacg aaaggctggg tcaaacccaa gaaccacgcg
     7501 ccctttcagt ggagcaacca agaggagatt gcgcagattg tcagaaaaaa gttggatagc
     7561 tccctcttca gctttctcag ttaattctgt ccgaatacga cgctcaatag caggcaagac
     7621 ctttttctta acggattgct gaacaacttc atcaatataa gcatttttca ccttgaaacg
     7681 agtagcaaag aaggcaagaa tacggtccgt cgcatgttca aaaccgatct tcaagacacc
     7741 aagtttctcc ccacgattga gagccaaggt acgatagcct tgcatagttc caactgtctc
     7801 tgaaaaatca taataaatct gaaaaacctg cttttcatca agactttcat ccttggcttg
     7861 agaagtgagt ttagagtgtc tcagcacttc ctgataagtc atagaacgca aggtcacatc
     7921 ttccgataag gcttcgacca aaatatcaac tgcaccggtc aaggcttcct tgccagtcgc
     7981 aaatccttca cagacgaact tttcagcttc tttctctaag tcaactatat tctgcaaaat
     8041 caagcgagca agaggaaaga gtccagcttc acgggcaatg gttgccttgg tactacgctt
     8101 ttccttataa ggaagataga gttcttcaac gtctgctaat ttttcggcaa ctaagatagc
     8161 ttcttccaat tccttggtca acttaccttg ttcttgaatc ttagctaaga cagcttcctt
     8221 acggtcattg agatttgtca gacttttatc caaatcaata atagccttaa tcgccacctc
     8281 atccagacta ccagtcatgt ccttgcgata acgcgcgata aagggaatag tcgccccttc
     8341 agctgtcaaa cttagaacgg tatcaatttg ctttaacgtc actcccaaat cctgagagat
     8401 tttttcatat tttttatcca taaatctatt ataccacaag ctaaacgttt caaattaact
     8461 cgtagaacat ttaaaaaata tgtaggaaat agatttatat gctacagcgc aataacttgc
     8521 acttaaagag cattgccacc tttttttaac caagccatga tatcaaaagt atttaatgga
     8581 tcagacataa tagccagttc tggaagatgt tcctgacctg gaataacaca ttgacttttc
     8641 aaatttttat atggacg
//
LOCUS       DAVKZB010000077         8517 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKZB010000077 DAVKZB010000000
VERSION     DAVKZB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.077"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..196
                     /locus_tag="VXA80_001508"
     CDS             <1..196
                     /locus_tag="VXA80_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEV0353985.1"
                     /translation="DNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNK
                     RIKVKLKGLIPVQYRTKSFG"
     gene            complement(324..527)
                     /locus_tag="VXA80_001509"
     CDS             complement(324..527)
                     /locus_tag="VXA80_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0353986.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(520..1602)
                     /locus_tag="VXA80_001510"
     CDS             complement(520..1602)
                     /locus_tag="VXA80_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0353987.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQTALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1728..2510)
                     /locus_tag="VXA80_001511"
     CDS             complement(1728..2510)
                     /locus_tag="VXA80_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV0353988.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2510..3853)
                     /locus_tag="VXA80_001512"
     CDS             complement(2510..3853)
                     /locus_tag="VXA80_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV0353989.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQIIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3952..5268)
                     /locus_tag="VXA80_001513"
     CDS             complement(3952..5268)
                     /locus_tag="VXA80_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV0353990.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5461..6687
                     /locus_tag="VXA80_001514"
     CDS             5461..6687
                     /locus_tag="VXA80_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV0353991.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6886..8460
                     /locus_tag="VXA80_001515"
     CDS             6886..8460
                     /locus_tag="VXA80_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0353992.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2430 a         1648 c         1895 g         2544 t
ORIGIN      
        1 agacaacggt atgatggagt ccttctttgg gattctgaaa tcggaaatgt tttatggcta
       61 tgagaaaaca tttaaatcac ttaatcaatt ggaacaagct attgtagact atattgatta
      121 ctacaacaat aaacgaatta aggtaaaact aaaaggactt atccctgtgc aatacagaac
      181 taaatccttc ggataaatta attgtctaac tttttggggg gcagttcatt ctacacccac
      241 tattatgcgt tttattgtgg gaagattttc ctcattttct agtgaaattg aactttttct
      301 aacatctttt gatagatgac ttcttaatca attatcattt ccattgttgt ttcttggggt
      361 ttcatccctt gtctttgata aaagcggaga gcaccctcgt tatcattcca gacacttagg
      421 gttagattat tgcagccaat ttctttagca taatagcaga aaaatgacaa taaaatagcc
      481 cctagatatt ttgaaaggaa agcatagggg ctatatagtt taatcaaaat aaagcaaatc
      541 tttgctggtg cttgtaaaga ggtcgaagcc atccttggta acaacaccgc agtcttcaat
      601 acgaacaccg actttaccag ggatatagat acctggttca acagagaagc acatgccttc
      661 ttcgatgacc atgtcgtttc cttccatgat agatgggaat tcatggacat ccataccgat
      721 accatgtccg agacggtggt tgaagtactc accataacca gctttttcga tgacctcacg
      781 ggcagcgcgg tccacttcat gagcagtcac acctggcttg ataaagtcaa gagcagtttg
      841 ttgggcttca agagtcaagt tgtaaatatc tttcttgaat tggtctggtt tgccgacagc
      901 gactgtacga gtcatatctg acgcatagcc attgaccaga acacccaggt caaagaggag
      961 aagagcatca ttttcaacct tattagctgc tggaatgccg tgtggattcg cagcattatc
     1021 accagtcaag accatggtat caaagctcat ttcgtaacct tcacgtttca tggcaaagtc
     1081 aatttgtgcg atgatatctg tctcagtctt atcaagagaa atattgtcaa aaccaacatg
     1141 aacagccttg tcagcataaa gacctgcaac catcattttt tgtacttcat cagctgattt
     1201 gatgaggcgc atgcgttgga tacgaggagt gaggttttca aactcagcag tttcaaaaac
     1261 tgttttcaaa ccatggtatt tggtcaagat gagattgtca aactcaacag cgacacgttt
     1321 gaagtcaagt tgtggaagag catgtttgat tttttgccat ggattttcag aatcgacata
     1381 gcccactact gggaaggaaa cggtgctact tgcacgttct acttcaagag ctgggacaaa
     1441 gaggagaggt tcctgatccg ctaggacaaa gaggaacatt tggcgttcat ggggatcact
     1501 gtaaaaacca gtgaggtaat tgattgtgac ggggtcagat acgacagcga cgtctagttt
     1561 ttctgattca agatatgtta ggatttgttg taatttagac atatgctacc ttctttctac
     1621 ccctctattt tggcaaaaaa tgagagaaaa tgcaagggag aagggtaaaa gaaaagacaa
     1681 aaaactccga caagataacg gagttctttg atatcatttt cctttgttta agaaaagtca
     1741 gccttgcttt tgacgtcact gtactcttca gcgatttctt ggctgaggat atcctcatag
     1801 gcagggagtt ggatatcctg accatatttc ttcttgaggg cagtagtaac taggcgataa
     1861 gccagattgg cattacgaga gaggataggc ccgtggaagt aggaaccaaa gacattctta
     1921 taatgaaccc cttcaccgac cttttcttcg ttgtttccat ttccatagac aacctgtccc
     1981 agcggttttt ggtcatcaga gaggaaggta cggccttggt gattttcaaa tccatagtag
     2041 gtttcatcga aatcttcatt gtgaatcttg atgtcaccga taaaacggtt attggtctgg
     2101 ttgagcgtgt agtgtcccat gacccctagc ccttcgatac gttttcctga agcttcaaca
     2161 taatattgac ccaatagttg gaaaccaccg cagatagcca gaactacacc gtcgttttgg
     2221 atgtagttgt caatgctctc ttttttagca ggtaggtcgt ctgcaatgat actttgttca
     2281 aagtcttgac caccaccgaa aaaggcgatg tcgtagtgat tttcatcaaa gtcatcatgg
     2341 agagaaacga tgtcaacggt cacatgggct cccagttttt cagccacata cttgagcatg
     2401 aggatgtttc cattgtcccc gtaggtattc atgagatttc cgtagaggtg ggcaatgttg
     2461 agctgatagg ggtaattgcc atcttttgag gaaagtgaag tataaaccat tagttcatct
     2521 cctttctaac aatctgacga ctagccagca gttcacgaaa ttccagcatg gcagtataag
     2581 ttgccagaat ataggcatgc ttgcagtctt gattctcaat ggtcttgaga acttgctcca
     2641 gattactcgt ttcagtgatt ttctcagctg gatagccagt cactcggagg cgacgagcga
     2701 tttcagaatg acgaacaccg ccagcgttga tttcaggaat gtccatgtca gtgatttgct
     2761 caaagtctgc atcccagatc cagctagtgt caattccatc tgcatagttg gcattaagga
     2821 ggacagatag gctaaatgga taaggtgcta gtttgatcat ttcgatagct tgggttgcac
     2881 cgactggatt tttaatcaag acaagggtac attccttgtc accgatatga aaggtttctt
     2941 ggcgtccaaa gacagcacgg ctcttgtcaa atccctgttt gatgatttgc gaatcggcac
     3001 ctaggaaacg ggcgatggcc acagcagcta gggcgttata gatattatag agcccgccga
     3061 tttggatacc gtattcttgg ccgtctatga caaagcgaga gcgattgttg gtcaactcaa
     3121 ccaattttgt caaacgatag tcgagatcag gacgtttaca tccacaacct tcacagatat
     3181 aggcacccaa gtttgcatag gtattatgct catatttgag gatgccttgg cagtcaggac
     3241 agagaatccc ttcggtattg tagtgagcca gttgggctgg tcctttttcc aagtcaaaac
     3301 caaaatactc tatagggttt ggaatagttg gcttgtagaa aagtggactg tctccgttaa
     3361 ggagaacagt agcagttgga actttccgaa tggcatccaa tatcatgtta taggtagtat
     3421 agatttcacc gaaacggtcc atctggtcac ggaagatatt agtaatgaca aaaagactag
     3481 gctggatata gtcacagata cgagatagac tggcttcgtc gatttctagg acggcaatat
     3541 ttttcccagt tttagaagat ttggctgtta agaaggttgt cgcaattcct gtaatcatgt
     3601 tggcaccgct tgggttggtc agaacttgac cataaacttc ttttaaaata ccgacagtga
     3661 gggcagttgt caaggttttt ccgttggttc cagtgaccac gacaatctcg tagttcttag
     3721 ctaggttttg taaaatatct ttatcaaatt gaagggcgac tttacctggg agcgtacttc
     3781 cacggccaag acggcttaaa atgaagtggg aagaacgccc agcaagaagg cccaaagtag
     3841 tttttaagtt catgtttcta ttatatcata aaagaggaga aagacagatt tgagattccg
     3901 cagaagtaaa aacagcccaa agagggctgt tcgatacgat taaatctcaa cttaaatcgc
     3961 aaacaagtca ttcaagttct cagccaaggt tgggtgagtg aagatttgtt ttgtgaagta
     4021 agtgtaagga atcttgttgt ccatagcaac agtgatgatg ttgatgattt cttgagaacc
     4081 ttctgagaag atgcttgctc caagaatttc ttttgtttca gtattgacaa cagctttgaa
     4141 ggcaccgcga aggtctccat ttacgtgacc acgaggcatt gctgcaacgg ggatttcctt
     4201 aacagcgtat ggaagtttca aatcagctgc ttggctttca gtcaaaccaa cttgtgaaag
     4261 tgcaggtgtg atgaacatag tatttggcac attgagacgg tcttcaagtg tgtagctgcc
     4321 atctccagca aggtagctgt aaacaacacg gaagtcatca agtgaaatgt aagtaaattg
     4381 aaggccaccg ttgacatctc caactgcaaa gacaccagga acgtttgttt gacagtgttt
     4441 gtctacttta atagcaccac gttcagttag ttcaatatct gtattttcaa gttgaagtgg
     4501 ttctacattt ggtttgcgtc cagttgcgta gagaagggcg tcgaaacggt aagtttcgtc
     4561 ttcagttacg acaagcactt ggtcaccatc gtttttgatt tcagtagtat ggatattttg
     4621 aagcaattca atgccatctt cttccaggta ttgtttagca agagctgcga tggaaggttc
     4681 tgcacgagga aggaaagtat ccaaggcatc taggactgtg accttgcttc caagtttgtt
     4741 gtaaaggccg gcaaattcaa gaccgatatt tccgccacca aggattccaa gtttttcagg
     4801 taatttgtcc aagttttgga tacctgttga gtcaaagatg tttttgcttg tagcaagtcc
     4861 agggattggc aagacgtttg aaacagcacc agtgttgatg acgattgttt cagcagtcag
     4921 ttctttcttt tcatcaccag cttggatttc gatgacttta tttgaaagga agtgagcttc
     4981 cgcatcaaag atatctacgc ctgtaccagc aacagtcgca tagtttttac cgttgaggcg
     5041 accagtgatc gtgtttttag tagcaatcac ttcttcaaaa gacaagtcct tttcagcagc
     5101 aactagcaag gttttagttg ggatacaacc aatgttgata caagttccac cgtacatagc
     5161 cttgctgcgt tcaacgaggg caactttttt gccagctgaa gccaatttac ctgctagtgt
     5221 tttaccagct ttaccaaatc caataacaat taaatcgtat gttaacattt atcttcctcc
     5281 tattcgaatt acattctagc acaagaaaaa aacttttgca caattctgct acggaaaaga
     5341 cagaaaaaga taaaaacgct ttccagacaa aaaactagta attttttgac aagcttgata
     5401 aactaaaatc ctatctattt ttatataaga tagaataaat gttcgaaaat aggaggtttt
     5461 atgaaatcga atcgttatat tattgccttt gctggggtca ttttacactt aatgctgggt
     5521 tcgacttatg cttggagtgt ttatcgtaac cctattattg aaaaaacggg atgggatcag
     5581 gcttctgttg cctttgcctt tagtctagca atcttttgtt tgggcttatc ggctgcattt
     5641 atggggcgtt tggtagaaaa atttggtccg agagtcatgg ggagtctatc tgcttttcta
     5701 tacgcaggtg gaaatatctt aacaggattt gcaatagacc gtcaggagct gtggttgttg
     5761 tatctcgctt atggcatttt aggtgggctt ggtttgggag caggctatat tacccctgtg
     5821 tcgacgatta taaaatggtt tcctgataaa cgtggtctcg caacaggttt agcgattatg
     5881 gggtttggtt ttgcttcttt attgactagt cccatagcgc aacacctcat cgcaggggta
     5941 gggattgtag aaacttttta tattttagga gcaagttact ttattatcat gctcctagct
     6001 tcacaattca ttaagcgtcc aaatgagcaa gagcttgcaa ttttatcttc ttcagggaaa
     6061 gaaaaaacag cctctttgac gcaaggaatg gctgcaaatc aggctctaaa aagcaatcgg
     6121 ttttatatgc tttggattat tttctttatc aacatagctt gtggtttagg cttaatttca
     6181 gcggcatcgc caatggcaca ggagatggct ggcttgtcta caagtcatgc agcagtaatg
     6241 gtgggtgttt tggggatttt caatggattt ggtcgcttgc tctgggcgag tttgtctgac
     6301 tatatcggtc gccctctaac ctttagtata ttactgcttg tcaatctttt cttttctctc
     6361 tcactttggc tctttacaga ttccgtttta tttgtagttg ctatgtctat tttgatgact
     6421 tgctatggag ctggtttttc tttgattcca gcttatctca gtgatatttt tggaaccaag
     6481 gaattggccg ctctgcatgg ttatatttta acagcttggg caatggctgg tttagcggga
     6541 cctattttat tagcagagac ttataaaatg gctcattcgt acacacaaac cttgttcgtt
     6601 tttctcattt tatacagtat cgccttggct ttgtcttatt atctaggtcg ttcaatcaaa
     6661 aaagaaagtc aaaaaccgct tacatgattt tctgtagaaa tggggctatc ttttgctctg
     6721 gtcttgtgtt atactagata ggttgcaaag aaaacagtac ttttcttttg tggaaaagaa
     6781 gcaacacgat tttatatcta gttatatgaa aatacgtcat aaaaagaaaa gtataaacta
     6841 agccctatag ggtggcttcg caccaccttt agaaagaaga ataacgtgaa atttaatgaa
     6901 ttaaacttgt ctgctgattt gctagcagaa attgaaaaag ctggttttgt agaagctagt
     6961 cctatccaag aacaaactat tcctttggcc cttgaaggca aggatgttat cggtcaagct
     7021 cagactggta caggaaaaac tgcagccttt ggcttgccta cccttgaaaa aatccgtaca
     7081 gaagaagcga ctatccaagc cttggtcatc gctccaactc gtgaactagc tgtccaaagt
     7141 caagaagaac tcttccgctt tggtcgtagt aagggagtca aagtccgttc agtatatggc
     7201 ggatcaagca ttgaaaaaca aattaaggct cttaaatctg gtgcccatat tgtggtggga
     7261 actccaggtc gcctcttgga cttgattaaa cgcaaggcct tgaaattaca agacattgaa
     7321 acccttatcc ttgacgaagc ggatgaaatg cttaacatgg gcttccttga agacatcgaa
     7381 gccattattt cccgtgtacc tgagaaccgt caaactttgc ttttctcagc aactatgcca
     7441 gatgccatca aacgtatcgg tgttcagttt atgaaagccc ctgaacatgt caagattgcg
     7501 gctaaggaat tgacaacaga attggttgac cagtactata tccgtgttaa ggaacaagaa
     7561 aaatttgaca ccatgactcg tctcatggat gtggcacaac cagaactcgc tattgtattt
     7621 ggtcgtacca aacgccgtgt ggatgaattg actcgtggtt tgaaaattcg tggcttccgt
     7681 gcagaaggaa ttcatggcga cctagaccaa aacaaacgtc ttcgtgtcct tcgtgacttt
     7741 aaaaatggca atcttgatgt tttggttgcg acagacgttg cagcgcgtgg tttggatatt
     7801 tcaggtgtga cccatgtcta caactacgat attccacaag atcctgagag ttatgttcac
     7861 cgtatcggtc gtacaggtcg tgctggtaag tcaggtcaat ctattacttt tgttgctcca
     7921 aacgaaatgg gttaccttca aatcattgaa aacttgacta agaaacgcat gaaaggtctc
     7981 aaacctgcaa gtgtagaaga atccttccaa tcaaaaaaac aggtagctct caagaaaatc
     8041 gaacgtgatt ttgcagatga aaccattcgt gccaactttg agaaatttgg taaggatgct
     8101 cgcaaattgg ctgctgagtt tactccagaa gaattggcaa tgtatatctt gagtctgaca
     8161 gtccaagacc cagatagcct tccagaagtg gagattgcac gtgaaaaacc actaccgttt
     8221 aaaccatcag gtaatggttt cggtggtaaa gctaagggag gtcgtggagg ccgtcgtggg
     8281 gacgaccgtc gagagcgtga tcgtcgtggc aatggtcgcc gtgatgagtt caaaaaagga
     8341 agtcgtggca acgatcgttt tgataaggaa aaacgttacc gtaaggataa taaaaaacca
     8401 cgcaatactt taagcgaaaa gcaaacaggc tttgttattc gtaacaaagg tgataaataa
     8461 gaaaaagcgg ttcaaaatga atcgcttttt tatataagga gaccgaatgt gaaaaag
//
LOCUS       DAVKZB010000078         8349 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKZB010000078 DAVKZB010000000
VERSION     DAVKZB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.078"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            16..507
                     /gene="tpx"
                     /locus_tag="VXA80_001516"
     CDS             16..507
                     /gene="tpx"
                     /locus_tag="VXA80_001516"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV0353993.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(603..3311)
                     /locus_tag="VXA80_001517"
     CDS             complement(603..3311)
                     /locus_tag="VXA80_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0353994.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLININVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(3313..4014)
                     /locus_tag="VXA80_001518"
     CDS             complement(3313..4014)
                     /locus_tag="VXA80_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0353995.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(4264..6675)
                     /locus_tag="VXA80_001519"
     CDS             complement(4264..6675)
                     /locus_tag="VXA80_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0353996.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLTKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGVSYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLTNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(6728..7420)
                     /locus_tag="VXA80_001520"
     CDS             complement(6728..7420)
                     /locus_tag="VXA80_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV0353997.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(7954..8334)
                     /locus_tag="VXA80_001521"
     CDS             complement(7954..8334)
                     /locus_tag="VXA80_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0353998.1"
                     /translation="MKLKRLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
BASE COUNT         2388 a         1961 c         1554 g         2446 t
ORIGIN      
        1 agattggagt atcctatggt aacttttctc ggaaatcctg tgagctttac aggtaaacaa
       61 ctacaagtcg gcgacaaggc gcttgatttt tctcttacta caacagatct ttctaaaaaa
      121 tctctggctg attttgatgg caagaaaaaa gtcttgagtg tcgttccttc tatcgataca
      181 ggcatctgct caactcaaac acgtcgtttt aatgaagaat tggctggact ggacaacacg
      241 gtcgtattga ctgtttcaat ggacctacct tttgctcaaa aacgttggtg cggtgctgaa
      301 ggccttgaca atgccattat gctttcagac tactttgacc attctttcgg gcgcgattat
      361 gccctcttga tcaacgaatg gcacctatta gcacgcgcag tctttgtcct cgatactgac
      421 aatacgattc gctacgttga atacgtggat aatatcaatt ctgagccaaa cttcgaagcc
      481 gcaattgcag ctgctaaagc cctatagaaa aaatcctctg tcacaaagag ttccctgctc
      541 tttgaaacgg aggatttttg aatattagta aatagaagtt caatcagcta aaaataacta
      601 ccttactcta cagatttcag ggcttctaac atatcaacct ttctcagata ataattgacg
      661 aagaaaccaa gcaaggtcaa aatgatgctt actgctgcca ctgggattac atagacttcc
      721 cagcctacct gcggataaaa gagaatagtc gcaggcgaaa tcatttgaat caaaaattgg
      781 tgtaaataga aaccagctac cagaccaagt acgattccca caagggacag cacaatcgtc
      841 tcacggtaaa tgtagagtgt gacttcatta ttatgaaaac cgagaacctt gatagtggag
      901 agttcacgga ttctctcagc tacgttgata ttgatcagat tgtaaaggat gacaatagct
      961 aatagaaccg acacgatgac taaaatggtc atggtctgat tgagtgagct agcgatagag
     1021 tcgaagagtc gaatggctga agcattttgg acaacgctgg acaccgcaga ttgattcata
     1081 agcaagcccg cctgactttc gatactagtt gcactggtat cccttaatga gaccagataa
     1141 gtgttggctt ggggtagctg tccgtaaagt tgctcatagc tagcctgact catataaata
     1201 aagtgaccaa cgtagttctc agtaatagca gcgaccttta gttccttacc ttcaatttct
     1261 aaagtctgcc caaccttgac acctgccagc tgggcgagtt tagctgtaat aacgatgcca
     1321 tcttttaatg tcagctcctg ctgatgatgt tgaagatgga taaagggagt caaatcttcc
     1381 ttctctatca tcataagagt aatgttttga agaccagcct tgcctttgaa atccttgtct
     1441 agcgttttag aatagatttt ctggtaggct agtatctcct gccctttcaa cacttctgct
     1501 agctctacct tgtcctgatt ggtcgcacta ggattttcag agacaagcat ctgatactgt
     1561 tggatttgtt gaaactgttt agacggaact cctgctacag aagattggat tcccaaacct
     1621 gcaaagagca gagctacaga acctgccaca ccaaagattg tcatcaacat tctctgctta
     1681 taacgaaaga tgttgcgggc tgttacctta tgagtaaaac tgagacgacg ccagataaaa
     1741 ccgatacgct ccaataagat tttagctcct ttgacaggag gtttaggaag tagaagctgg
     1801 gctgcttcgg catgaagttc cctccaagcc accagatagg ctggtaacac actcgccaac
     1861 cagctcaaga caagagctag taagctatag gtccaataga actgaatctg ggtttctccc
     1921 accaccatgc cttttgtaat gacacttgaa attacactgg ctagcaaata atgaccaagt
     1981 atactaccta gagccgttcc gacagtccca gctactagtc cataaaggag aaacttggcg
     2041 ataatatcct tactacggta acccaaggcc ttaaaaatcc ctgcatgagt tcgctcttcg
     2101 tctacaaagc gagtcatggt cgtaaaggtc accatggctg ctacggcata aagtaccaca
     2161 ggaaagatat tgcccactgc tcgaatactg gatgaagcat tgctatacat aagatagccc
     2221 tgaccacctg gcatggtctg acgattatag acctgatacc ttggctccgc caaatcgtcc
     2281 aagacttgct gatgtttttc taatttttcc ttttcttggg ctagattttg ttcagcttgc
     2341 tttagtttat cctcttcctt gcccaattcc tgcttggctt gggtaagttg agcactagcc
     2401 tgctctctct gaacttgagg aaacaaggct agttgacttt cttgagcctg tatacgagct
     2461 tgagcagctg ctaaacgacg cttgccttcc tgcaaattag tctgagcctt gtcaagggtc
     2521 tcttgcccct tgtctagaga ctcttgtcct tcttttttca aaagttgcag acgtaccttg
     2581 ccattatctg ataaagtttg ttcaaggtct tcctgatgtt gcttggattt ttcttcataa
     2641 gctgatgaaa aggcatttaa acctgctaaa tcttgatatt tcaaacgagc tatattgtag
     2701 actttctgat caaactgact aggtaaaatc accccatagg ctgtcagagt cccacttcca
     2761 cttcctgcgt agcccatatc tcgctgggag aggatttcag ccgaatccac aaaaccagta
     2821 atggtataag tatggtcttt taaagaggaa tgaccctctt ctttttcttt aaaactaatc
     2881 tcctgtccca cgctgtattg gccttgcaaa tgagtggcca aagcgatttc cttgtctgac
     2941 tgaggaagtc gtccctttct tagctgaaag gttgaaattc gctctggttt ggagtacagc
     3001 cgaatggcat cctgcccatt atccatagtc acatctgtca aatagccaaa ctcgacctct
     3061 gcgccctccg tctgttttag ttcttcttgg tctgcttgat ccaagccata gttagacatg
     3121 actgccaaat ccaaggtttg agcagttgtt aaataagcat tagctgtcgc ctccatgttg
     3181 ggactggtta ctttgaggcc tactaaggct agagatccca acatcatcag gatcaagatg
     3241 gataaaaaac gccccttgga gcctgtgaag gactgaatta agtccttcca ataagttttt
     3301 cgcttgatca tactagtact ccaaactgtc aatatcctga ggatgctggt tgagcaccac
     3361 atccttgaca ctggcatcgt gcatatgaat cacgcgatca gcaatgggcg ccaaagctcc
     3421 attatgagtc acgatgatca ccgtcgctcc cttttgacga gacatatctt ggagaatttt
     3481 caaaacctgc ttgcccgtct gataatccaa ggctccagtc ggttcatcac aaaggagaat
     3541 tttaggattt ttggctaccg cgcgtgcaat ggagactcgc tgttgctccc ctccagaaag
     3601 ctgggctgga aagttattga gacgatgagc cagacctaca tctgtcaaga cctgatcagg
     3661 attcaaggca tctgtcacaa tttcagaagc cagttccaca ttttccttag ctgtcagatt
     3721 agaaactaga ttataaaact gaaaaacaaa ccccacatca tttctacggt aattggtgcg
     3781 ctggtgggaa ctataatccg caatattaac accatcaatc cagatttccc cttcatcatt
     3841 ggtatccatt cccccaagaa ggttaagaac tgttgacttg cctgcacctg aagcaccaag
     3901 gataataacc agctccccct tttcaatctc aaaattcaca tcacgattgg ccacaatctc
     3961 cgtgtcccca acctgataac gcttgtaaca gtgtttcatc tcaatataag ccatcagacc
     4021 caccctctat caaacaaatt atactcctcg aaaatctctc ttcaaaccac gtcagcttcc
     4081 atctgcaacc tcaaaacact gttttgagca acctgcggct agcttcctag tttgtgctct
     4141 ttgattttca ttgagtatta cttcctatta tcattataac gctttttcaa ctagttgaaa
     4201 actgcttata ttcttaaatc cttattaaaa ggcagtttca aattactgcc ttataacatc
     4261 ttcttaaaaa taaaattgaa caagatcacc ttctgctgtt gccaccgaaa tacaatcttt
     4321 ccccatgcat ttcgctttta ttttttcttg attcatttta atcacactct tctcaatatc
     4381 tggataagaa actcgcaaat ctcctccact ccttgataaa atggtcaact gtaagagttt
     4441 tttatcttcc cagctcatgc tcacttcaaa atgtccacgt gccattaagc ctgaaacaga
     4501 accggttgac caagcatcag gtagggcagc tagaggtacc agataagctg catgagactg
     4561 gagtaacatt tctgccatgc cactagtagc accaaaatta ccatctatct gaaaaggagg
     4621 atggctacac caaagatttg tcaaggtgga tgtctttaac tgctctgcca ataatttatg
     4681 ggctcgattg ccatctccca aacgcgccca gagattgatc ttattagcct tggaccagcc
     4741 tgtgccgcca tctccacgat cattgaggct agcacgtgcc gcttcaatat actcttgtcc
     4801 cttgtagcta aagagattgc caggatagag tcccactaga tgggaagcgt gccggtgctg
     4861 ggcctccact ttctcatttt gaaaatactg ctcttcctcc tcataccact ccctgattcg
     4921 accagattga gtgatttgca aaggatttag taaatcagac ttctccttaa cctcagtcaa
     4981 caagtcctca tccagtccca attcctgagc agcctgaata aaatcatgaa ataactgcca
     5041 aatcagagat tggtcataag tattgccaat cgaaatcggc ccatgttctg gggaataaga
     5101 cggagaagac acccaacgct gcgcctgctg atccttatgt aaaaaggcat tccaaaaacg
     5161 aaccgtttcc ctcaacatgg gataaatttt ctctctgaga tagtcttggt ccctataaaa
     5221 tgaataggct tcataaacgg tttgcatcat ccacgcattg gcagctggtg accaacccca
     5281 atagtaatcc caaccaggtg ccgtccaacc aaagggagtc gcttgagtat gaaccaacca
     5341 accattctcc tcacctttct gagagacgat tcctgcatac tttacagccg ctagacgacc
     5401 atagacacgc aaatcatcta catagttgat gactggaaag accgtctcta ggagattggt
     5461 aacataggct ggccaataat tcatctgcag attgacattt aagtgatagt ccgaattcca
     5521 aggaggattg tcgaccgcat tccagactcc ctgtaggtta gctggtagag catctgggca
     5581 gtctctggac gaactaatca ataaataccg tccatactgg aagaacagct cctccaaagc
     5641 ctgcccttct tgtggcttat aattttttaa caaatcatct gtagtggatg cgtcaacatc
     5701 agcttccaaa tccaattgaa cacgctggaa taaggcttgg tagtcctcga tatgccttga
     5761 tttcaattgg gtatagccct tttctttagc tgtgtccacc aagtctatca cctgttgctc
     5821 taaatctagt ttcttgcgat aattgctagc aggattttgg gcaaaatccg tcttagcggc
     5881 caagaagaga ttggcataac tgactcctga tatctgaacc ctatctgacc aaactctaat
     5941 atctccatcc gtttcccaag ctagataact agcaaaccgc agatcattat ccttaactct
     6001 tcccttcatc aagatatgag aatcagtaat atccaactta cactccttgt aatcagattt
     6061 ttcctgctca tactttccat cagaagccaa atcacaggtc aaggatagtt ctatagtaaa
     6121 atctagagtt tccaaccctt ccttagtaaa acattgaatc aagagatcat ctggaaaact
     6181 cgcaaaagct ttacgttcaa atcgcgttcc cttatagaca taagaagtcg tcgcaagtgc
     6241 cttactaata ttcagctgtc tctgatagtc cgtcacctga gacaaagtcg taccttgctg
     6301 gctgaactca atgtgaatat ccccaaaaga cagataggtc ccatattgac tcgtttttgg
     6361 cccaattagg tgctgctcag ccagttcctt agtcagattg taatctctct tctccaaagc
     6421 ctgccgaatc tcagctaaaa aaacatactg atcctgaaga tttccaccct gataatctga
     6481 actatcagga agtggacctc cagaccagag acttttttca ttaaattgaa tccgttcagc
     6541 ccctataagg ccaaatactt ttgctcctaa agaaccattg cctatcggta aagcttcttc
     6601 ttcccaaccc atgtaagtgg ttgaagctgg ttgcttgtag gccagtacat aatcttgttt
     6661 tttattcctt atcatacttc catactaaca taaaaagcct agaaatcaat cctaggctta
     6721 caatttttta ttttccaagg tagtattcag aaacaacgtt caatttttcg tcgaattcga
     6781 ataccaatgg tgggaagtta gggatttcca cgtccatgat ctcatcatct gacaaacctt
     6841 tgatgtgttt tacaagggca cggattgagt taccgtgagc tcctacgaat acgtttttac
     6901 catctttaag agctggagcg attttatctt cccagaatgg aagagcacgt tccaaagtca
     6961 ctttcaagtt ttcagcatct ggaatgactg agtcgtcaag tgaagcataa cgacggtctg
     7021 tgtgagctga gtgctcatca tcacggtcca tatttggagg caatacatcg tatgaacgac
     7081 gccagatgtg aacttgctca tcaccaaatt gttcagcagc ttcagctttg tttttaccag
     7141 tcaaaccacc gtagtgacgt tcgttcaagc gccatgattt ttcaactgga acccacaatt
     7201 ggtcagaagc ttcaagagcc aagttagttg ttttgatagc acgtttcaat actgaagtgt
     7261 aagcttggtc aaattcgata ccagcttctt tgatcaattt accagcgtca atcgcttgtt
     7321 gtgtaccttt ttcagacaaa tcaacatcag cccaaccagt gaaaaggtta gctttgttcc
     7381 attcagactc accgtggcga gcaaaaacca attttaccat tagatggatt ctcctttaaa
     7441 ttttccgagg tttcccctcg tttatctatt ctattttaca caatttttca caaaaaagct
     7501 agtcaaaacc aaaaaggaaa ggactgtttg acaatgtttg gcaatcacat tgtttttggt
     7561 tgtttctatt ctatcttcat tatttaacct ttttttctgc gcatcgaata atttttgagt
     7621 tgactctttt agtttagtaa acgagtcatt attttccata gttgtgcctt tgaaaccttc
     7681 ctcagcttta gtaaaataat catcaaaaat atttttataa agatctatat aatctgcata
     7741 agtgcgtatt gattcaatcg catcgtctgt tacaaccggt gtatcgaaag tgattgtttc
     7801 ttttgaacta ttggatgttg ctactgtctt ctcatcatta cttgttgaag tattctttga
     7861 actttatgtt ccacatgcaa ctagaagaga aagagataat agagctactc ctataccgat
     7921 aaactttttg ttcttttcat gctaaaatac cttttattct tctgatttga atttcttcaa
     7981 tgttgcaagg aatagagatc ctccgataat agcaagaatc cccccaaccc atcctaagaa
     8041 tggaatgaga ctaactccac cagaaacaat cattagtaca gacggagctg cacctacacg
     8101 cttatctcct ttatagtaaa caatagcaat gataccaaga acaagatttg caatcttcaa
     8161 ggcattcaag agtaaggcta cacctgaaaa agcccctgca gtagctcctc cattaagagt
     8221 tgtagcagct gtgtttaccg ctgttcccaa caagacaaaa ggaccaataa gaagtaaaat
     8281 tcctcccaca agaccgacga taccactaat taaagcaagt ctttttaatt ttataataaa
     8341 atctccttt
//
LOCUS       DAVKZB010000079         8333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKZB010000079 DAVKZB010000000
VERSION     DAVKZB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.079"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            60..923
                     /locus_tag="VXA80_001522"
     CDS             60..923
                     /locus_tag="VXA80_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0353999.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1306..1926
                     /locus_tag="VXA80_001523"
     CDS             1306..1926
                     /locus_tag="VXA80_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354000.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2013..2456
                     /gene="tsaE"
                     /locus_tag="VXA80_001524"
     CDS             2013..2456
                     /gene="tsaE"
                     /locus_tag="VXA80_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV0354001.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2446..2964
                     /locus_tag="VXA80_001525"
     CDS             2446..2964
                     /locus_tag="VXA80_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354002.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2972..3988
                     /locus_tag="VXA80_001526"
     CDS             2972..3988
                     /locus_tag="VXA80_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0354003.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4073..5329
                     /locus_tag="VXA80_001527"
     CDS             4073..5329
                     /locus_tag="VXA80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV0354004.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5384..6550
                     /gene="recA"
                     /locus_tag="VXA80_001528"
     CDS             5384..6550
                     /gene="recA"
                     /locus_tag="VXA80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV0354005.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAA
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6857..8227
                     /locus_tag="VXA80_001529"
     CDS             6857..8227
                     /locus_tag="VXA80_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0354006.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVATTWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         2521 a         1417 c         1838 g         2557 t
ORIGIN      
        1 tattttgaac taaaaatttt ataatgaaat taagcaaaca ggaaggttta ttatctttaa
       61 tgaatacact cgcagagaaa ttcagattga aaagaaaaga gttgagactc tcccagcaaa
      121 ctcttgcaga aggaatttgt gaacaaagcc agattagtaa aattgagaga gggcatttca
      181 ttccctccgc agaccttttg ttcaaactct ctcaacgact tgaagtacca ttagattatt
      241 tttttaatga acaaattgaa attaaatcta acctctctaa tttcaagcaa ttgtctgctc
      301 gactattaga cgatagaaat tatgacgatt tggaatatat ttatagaata gagattgaac
      361 gaagtacttt tctaacacta gaagaccgaa cttaccttga atggattaaa gctattattg
      421 acttctatca atatgacagt aagtgtgagg caatttcttc attggaaaat atattattaa
      481 aagtctcttc aaatactctg atttatttaa aggcgttgaa tactctatct aatttctatt
      541 ccttagtggg tcgtgaacaa gaatatgagg caaactactc tcatttaata gagttgtatc
      601 agacaaaaaa tttggaccat caagagtttt tatttggcta catcagagtt cgttacaact
      661 acgctcacta cctagtatca aaggaaaaat ataatgaagc catccaagaa gctcttgaaa
      721 cgattgaact ctgtaaacaa agacagacaa gctaccaatt ggctcctcta cttattcttg
      781 taggaaatgc tggagccaaa tttctagaca gagaacaagt caaaaattat tatatagaag
      841 caagagagtt atgtaagatt tataacaatc ctttaatgtt gatgaagata gaaaattatt
      901 tgaaggaact agatactgtt tagttaatct tgacattata gtttttctga aacgttaaat
      961 aacctgtaag gctgatagtt agattaatac tatcagcctt ataattatgt gactctgagt
     1021 ccgtctcgct ccgtgaggtg cgagacaaaa caaccacccg tttgacggat tttcatgatt
     1081 tcaaatttat attcttcgaa aatctcttca aaccacgtca gctctatctg caatctcaaa
     1141 acagtgtttt gagcaacctg tggctagcta cctagtttgc tctttgattt tcattgagta
     1201 ttagtctcta gtgtttgttt agcgtcttaa agagacattt gagaagatta gaaacagttc
     1261 tctcttttct cctagtataa tgattggtag gaagggagag agaagatgaa atcaatgaga
     1321 atcttatttt tgttagcttt aattcaaatc agtttgagta gctgtttcct atggaaggaa
     1381 tgcatcttgt cctttaaaca aagtacagct tttttcatcg gaagcatggt tttcgtttca
     1441 ggaatctgtg ctggagtaaa ttatctttat acccgtaagc aagaagtcca tagtgtccta
     1501 gccagtaaga agtcggtgaa gcttttttac agtatgttac tcttaattaa tttgttagga
     1561 gctgttcttg ttttgtcaga taacttgttc atcaaaaata cgctgcagca agaattagtt
     1621 gactttttat tgccatcctt ctttttccta tttgggctag atttgctgat ttttttaccc
     1681 ttgaaaaaat acgtgcgcga ttttcttgct atgctggaca gaaaaaagac agtgttggtg
     1741 actattttag caacacttct tttcttaaga aatccaatga ccattgtctc acttctgatt
     1801 tatattggac tgggcttgtt ttttgcagcc tatcttgtcc caaattcggt taagaaggaa
     1861 gtttcctttt atggtcatat tttccgagat cttgtattgg tcattgttac gctcattttc
     1921 ttttagagaa agcctgtttt tgtatggatg ttatgatgat aagtttagta ggaaaataga
     1981 cacaaaagaa aagtttttgg tataataaga gtatgtacac aaaaaatgaa gaagagttgc
     2041 aagccttagg ggagcgtttg ggccatctat tagcaaagaa tgatgtttta atcttaactg
     2101 gagaactggg tgcaggtaaa acgaccttta ctaaaggact tgcaaaagga ttacagattt
     2161 ctcaaatgat taaaagtccc acctatacta tcgtgagaga gtatgaaggt cgacttccac
     2221 tttatcacct agatgtttat cgtattgaag gagatgctga ttctatcgac ttggatgagt
     2281 ttatctttgg tggcggcgtg actgttattg agtggggaaa tctcttagga gatgccttgc
     2341 cagatgctta tttagaattg gaaattctaa aagaagcaga tggacgccgt ttaaattttc
     2401 aggcaaaggg tttgcgtgct gagaaattgt tagaggagct tcaatatgga gtatgaattg
     2461 ctcattaggg aagcagagcc caaagatgca gctgaattag tggccttttt aaatcgtgtg
     2521 agtttggaga cagactttac cagcctagac ggagatggta ttctcttgac tagtgaggag
     2581 atggaaatat tcctcaacaa gcaagctagt tcggacaatc agataacctt acttgcattc
     2641 ttaaatggca aaattgctgg tattgtaaat attacagctg atcagcgtaa gagagtccgt
     2701 catattggag atctcttcat tgtgattgga aaaagatatt ggaataatgg cttgggaagt
     2761 ttgttgctag aagaagcgat agagtgggca caagcaagtg gcattctgcg tcgtctccaa
     2821 ctgactgtcc aaactcgtaa tcaagcagca gtccatcttt atcaaaagca tggctttgtc
     2881 attgaaggta gccaagagcg tggggcatat atagaagaag ggaaatttat cgatgtttac
     2941 ttgatgggta aactgatagg ttagtagaaa tatggttaaa aaaattattg gaatggtgct
     3001 agctttactt tctgtaactg tagtaggagt aggtgttttt gcttatacta tttatcaaca
     3061 agggacagaa accttagcta aaacctataa aaaaatcggc gaagaaacca aggttattga
     3121 agcgactgaa cctctaacca ttctgttaat gggagtggac accggaaatg ttgaacgaac
     3181 tgaaacttgg gtcggtagaa gtgatagcat gatcttgatg acagtgaatc ctaaaacgaa
     3241 aaaaacaaca atgatgagtt tagagcggga tattctgacg cgcattgaat cagggaatgg
     3301 tcaggctcat gaagcgaaac tgaactcagc atatgcagat ggtggagcag agcttgctat
     3361 agaaaccatt caaaaaatga tgaatatcca tattgatcgc tatgtgatgg tcaatatgag
     3421 agggttgcaa aaactagtgg atgcagtagg aggtattaca gtcaataata tcctaggttt
     3481 cccaatttct atcagtgacc aagaagaatt taataccatt tctatcggtg ttggggagca
     3541 acatattggg ggagaagaag ccctagtcta tgcacgaatg cgttaccaag atcctgaggg
     3601 ggattatggt cgtcaaaaac gtcaacgtga agttattcaa aaagtcatgg aaaaagctct
     3661 cagtttaaat agcattggtc attatcaaga gattctaaaa gctttgagtg acaatatgca
     3721 gaccaatatt gatttgtctg caaaaagtat ccctaacttg ctaggctata aagattcatt
     3781 taaaaccatt gaaactcagc agttgcaggg tgaaggagag atacttcaag gtgtttctta
     3841 ccagattgtt tcgagagcac atatgttgga aatgcaaaat atactccgac gttctttggg
     3901 acaagaagaa gttactcagc ttgaaaccaa tgcggtttta tttgaagatt tatttggcag
     3961 agcacctgtt ggtgatgaag ataattaact tttgatacaa ataaaaaaat caatcgtagg
     4021 aaaattcctg cgattttttc aaaaaaatac gaatagatag gtaggaggaa acatgaaagc
     4081 agaaatcatt gctgttggaa cagagatttt gacaggacag attgtcaaca ccaatgccca
     4141 gtttttgtcg gaaaaactag ctgagattgg ggtagatgta tattttcaga cggctgtagg
     4201 agacaatgaa gttcgtctct tgtctttgct tgagattgcc agtcaacgta gcagtctggt
     4261 gattttgaca ggcggtttgg ggccaactga ggacgaccta accaaacaaa ccctagctaa
     4321 atttttaggg aaagcattag tctttgatcc tcaggctcag gagaagttgg atatcttttt
     4381 tgccctgcga ccagactatg cccgaacacc gaataacgaa agacaagctc aaattgtaga
     4441 aggagcgatt ccactgccaa acgaaacagg actggctgtt ggaggaaaat tagaagtaga
     4501 cggagtgacc tatgtcgtcc ttccaggtcc gccaagtgaa ttgaaaccca tggtcttaaa
     4561 ccaacttcta cccaagttga tgacagggag caagctgtat tcccgagttc ttcgtttctt
     4621 tgggattggc gagagccagc tggttacgat tttggctgat ttaattgata atcagatcga
     4681 tcctaccttg gccccttatg ccaagacagg agaagtcact ctacgtctgt caacaaaggc
     4741 tagcagtcaa gaagaggcga atcaagcgct ggatatcttg gaaaatcaaa tcttggactg
     4801 ccagactttc gaaggaattt ctttacgaga cttttgctat ggttatgggg aagaaactag
     4861 tttagccagc attgtggtag aagaactgaa aaggcaaggg aaaaccatcg cggctgcaga
     4921 gagtttgacg gcaggtcttt tccaagctac cgtggcgaat ttttctggag tttcaagtat
     4981 atttaagggt ggttttgtga cctatagctt ggaggaaaaa tcaaggatgt tggatattcc
     5041 tgccaagaat ttggaagaac atggtgtggt gtctgaattt acagctcaga agatggctga
     5101 gcaggcacga agcaagaccc agtctgattt tggaattagt ttgactggag tggcaggacc
     5161 agatagccta gaaggacacc cagttgggac agtcttcata ggcttggcgc aagatcaagg
     5221 aactgaggtt atcaaggtta atattggagg cagaagccga gcagatgtac gtcacattgc
     5281 ggttatgcat gcctttaacc tagttcgcaa ggctttatta agtgactaac ttttgatata
     5341 atagtagata ggtctgagga tcattagaat gtaggagaat agaatggcga aaaaaccaaa
     5401 aaaattagaa gaaatttcaa aaaaatttgg ggcagaacgt gaaaaggcct tgaatgacgc
     5461 tcttaaattg attgagaaag actttggtaa aggatcaatc atgcgtttgg gtgaacgtgc
     5521 ggagcaaaag gtgcaagtga tgagctcagg ttctttagct cttgacattg cccttggctc
     5581 aggtggttat cctaagggac gtatcatcga aatctatggc ccagagtcat ctggtaagac
     5641 aacggttgcc cttcatgcag ttgcacaagc gcaaaaagaa ggtgggattg ctgcctttat
     5701 cgatgcggaa catgcccttg atccagctta tgctgcggcc cttggtgtca atattgacga
     5761 attgctcttg tctcaaccag actcaggaga gcaaggtctt gagattgcgg gaaaattgat
     5821 tgactcaggt gcagttgatc ttgtcgtagt cgactcagtt gctgcccttg ttcctcgtgc
     5881 ggaaattgat ggagatatcg gagatagcca tgttggtttg caggctcgta tgatgagcca
     5941 ggccatgcgt aaacttggcg cctctatcaa taaaaccaaa acaattgcca tttttatcaa
     6001 ccaattgcgt gaaaaagttg gagtgatgtt tggaaatcca gaaacaacac cgggcggacg
     6061 tgctttgaaa ttctatgctt cagtccgctt ggatgttcgt ggtaatacac aaattaaggg
     6121 aactggtgat caaaaagaaa ccaatgtcgg taaagaaact aagattaagg ttgtaaaaaa
     6181 taaggtagct ccaccgttta aggaagccgc agttgaaatt atgtacggag aaggaatttc
     6241 taagactggt gagcttttga agattgcaag cgatttggat attatcaaaa aagcaggggc
     6301 ttggtattct tacaaagatg aaaaaattgg gcaaggttct gagaatgcta agaaatactt
     6361 ggcagagcac ccagaaatct ttgatgaaat tgataagcaa gtccgttcta aatttggctt
     6421 gattgatgga gaagaagttt cagaacaaga tactgaaaac aaaaaagatg agccaaagaa
     6481 agaagaagca gtgaatgaag aagttacgct tgacttaggc gatgaacttg aaatcgaaat
     6541 tgaagaataa gctgttaaag cagtggagaa atccgctact ttttcgattt ttgattcaag
     6601 tttttagatt atatatagta gcttgaaata agatatgaac aactttatta ggaaagtcaa
     6661 attaatttct agaaatgttt tagcagctac agcgtactat tccaaactca accaactata
     6721 atagatcgaa actagaatag tacacatcta cttctaaaac attgttaaaa atcgatttga
     6781 ctgtccttat ttcattccgc tatatataag tttgctgttt cttgtcgctc ctctggaaag
     6841 ctgatataat agctttatga ataaaaaacg aacagtggac ctgatacatg gttcgattct
     6901 tccctcgctc ttaagcttca cctttccaat cttgctatca aatatttttc aacagctcta
     6961 taacactgct gatgtcttga ttgttggacg atttcttggt caagaatcct tggctgcagt
     7021 aggagcgacg acagcgattt ttgacctgat tgtaggtttt acacttggtg ttggcaatgg
     7081 catggggatt gtcattgctc gttattatgg ggctcggaat ttcactaaaa tcaaggaagc
     7141 agtagcaacc acctggattt taggtgctct tttgagcatt ctagttatgt tgctgggctt
     7201 tcttggcttg tatcctctct tgcaatactt agatactcct gcagaaattc ttcctcaatc
     7261 ttatcaatat atttctatga ttgtgacctg tgtaggtgtc agctttgctt ataatctttt
     7321 tgcaggcttg ttgcggtcta ttggtgacag tctagcagcc ctgggatttc tgattttctc
     7381 tgccttggtt aatgtggttc tggatctcta ttttattacg caattgcatc tgggagttca
     7441 atccgcagga cttgctacca ttatttcgca aggtttatca gcggttctct gcttttatta
     7501 tattcgtaaa agtgtgccag aactcttgcc acggtttaaa catttcaaat gggacaaaag
     7561 cttgtacgcg gatctcttgg agcaaggttt ggctatgggc ttgatgagtt caattgtatc
     7621 tatcggcagt gtgattttac agttttctgt taatacattt ggtgcagtga ttattagtgc
     7681 ccagacggca gctcgacgca ttatgacctt tgcccttctt cctatgaccg ctatttctgc
     7741 atcaatgacg acctttgctt ctcagaatct aggagctaag cgacctgacc gcattgttca
     7801 aggtcttcga atcggcagtc gtttaagtat atcctgggca gtttttgttt gtattttcct
     7861 cttttttgcc agtccagcct tggtttcctt cttggctagt tcgacagatg gttacttgat
     7921 agaaaatgga agtctctatc tgcaaatcag ttcagccttt tatcccattt tgagcctctt
     7981 gttgatttat cgcaattgct tgcagggctt ggggcagaag atccttcctc tagtttctag
     8041 ctttattgaa ctaatcggaa aaatcgtttt tgtggttttg attattcctt gggcaggata
     8101 taagggtgtt atcctttgtg aacctcttat ctgggttgcc atgacagttc aactgtactt
     8161 ctcactattc cgtcatccct tgataaaaga aggaaaggca attttggcaa ccaaagtgca
     8221 atcctagttg gatttactga ataaaatcca tttcctctag tgaaaatcga aaaaacttgt
     8281 gttctcttct ttagtttggt gttgaaaata gtttaacaga cttttgactt ctt
//
LOCUS       DAVKZB010000080         8075 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKZB010000080 DAVKZB010000000
VERSION     DAVKZB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.080"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            303..479
                     /locus_tag="VXA80_001530"
     CDS             303..479
                     /locus_tag="VXA80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354007.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            880..3093
                     /locus_tag="VXA80_001531"
     CDS             880..3093
                     /locus_tag="VXA80_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV0354008.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHTTDIPARFGSGWTGYSWNRSLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAERLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(3315..3791)
                     /locus_tag="VXA80_001532"
     CDS             complement(3315..3791)
                     /locus_tag="VXA80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV0354009.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLISRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            4014..7217
                     /locus_tag="VXA80_001533"
     CDS             4014..7217
                     /locus_tag="VXA80_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV0354010.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASIRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7596..7981
                     /gene="tnpA"
                     /locus_tag="VXA80_001534"
                     /pseudo
     CDS             7596..7981
                     /gene="tnpA"
                     /locus_tag="VXA80_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2703 a         1272 c         1723 g         2377 t
ORIGIN      
        1 tttaagatat tcttctctgc tttttcatat tttttcgcta tgtagggtat gaagcaaaat
       61 agaaagtagt tcgaatagtt gataaacagg aaactgctgt tatttaaaaa aaaacaataa
      121 agtaaagtat gttatcgtag aatacgatac aatagtaaaa aagcaaaaag aatgcgtttt
      181 atatttgggt ttgaggttta taaaatagga taagcgtgct gaaaacttag atatgacaaa
      241 caaaataaaa tttttaaaag ctacagagaa acatggtata atgtatctaa aggaggtagc
      301 atatgtcctt agatatagat aaagaaaaga tgacaatcat gggaatagct tttgaaaaca
      361 gatccgtttt taagagtgtt tggtatgctt taagtacaaa tatgattgaa ggatggcgac
      421 ctacggttag tgatgttgaa aaattacgag atgaagctct tgctctgggg atgacttaat
      481 gaagcgcaac tgttatgaat cttatgacta tgttgatccg gataatttat ataccgatcc
      541 gggttcttca gttctaagaa ataagcagga aggaaaaaag cacgtgagct tgaatatcgt
      601 atgattgcta gtaagagttt gaaattattt atcaatccta tcttagttaa tagtttaaaa
      661 gtagtttgtt tcacaactgc ataagcttag aaaagttgaa aagtgataac tctttaataa
      721 actcttggtt tgttaaaaga tattttgtcc actgcatttt ttcaattgac tattatttgt
      781 catagatata acagatagaa cattgcattc agaataatga aaaacgcatc aaaaatggtg
      841 cgttctgttt ttatgctacc ataaaaggag gaaaacaaat tgaaaatttt taagggagag
      901 ttttatcgaa tctctgtatt aacagacaag ctagtaaggt tagaatactc tcgaactgga
      961 aattttgagg atagaacgac acaacttatc tataatagag attttggcca aatttcgtta
     1021 gattatatcg agacatcaaa tgtactagat attatgacgg actattttca tctgcacttt
     1081 aataaaggag aatttaacgc cgaaaattta tttatagaat taaaaggaaa ttttgccgta
     1141 tatggtagtc gctggtattt tggtgaatct attgaaacgt taaaaggaac agctcggact
     1201 ctggataagg cagatggagc aatctcgtta gaagatggaa ttattagccg aaatggtata
     1261 gccttattgg atgattctca aggatttatt tgggatgaac aatctggtta tattgagaga
     1321 gaaaatcaaa ttgacctgta tttctttgcc tatgggcatg attatagagg agcaatcaga
     1381 gacttttacc atttgactgg ttcaacaccc ttgttgccaa gatatgcttt aggcaattgg
     1441 tggagtagat attggcctta tacgtcggat gaatacttgg atttaataga cagatttgaa
     1501 acagagaaaa ttccattatc tatcggtgtg ttagatatgg attggcatac aactgacatt
     1561 ccagctcgtt ttggaagtgg ctggacagga tatagttgga atagaagctt aataccaaat
     1621 ccagaacagt tattgcaaca acttcatgat agaaagttaa aactctcctt aaatgtccat
     1681 cctgctgatg ggatacgggc ttatgaagaa gcttatcctc aaatcgcaga acggttgggg
     1741 ttaaatgtag aactagaaga acctgctatt tttgattttt ttaatccctc ttttagggaa
     1801 gcctacttta aagatgttca ttatgagcta gaaaagcagg gagtagattt ttggtggatt
     1861 gactggcaac aagggacaca aggtatgcta gatccacttt ggcttttaaa ccattatcac
     1921 tatcaggata gttgtaaaaa tgcagaaggt ggtttgattt tatcaagata tgcaggtcct
     1981 ggtagtcacc gctaccctgt tggtttttca ggggatacta ttattagttg gaattcctta
     2041 aggtttcaac cctattttac agcgacagca tctaatatcg gttatagttg gtggagtcat
     2101 gatatcggtg gacatatgtt gggggattat gacgaagagc tacaaactag atggctacag
     2161 tttggtgttt ttagtccgat tactcgatta catagttcta gaagtccttt taatagtaaa
     2221 gaaccttggt tcttttcaga aacaacatct aagattatga agaaatacct tcgtttgaga
     2281 catcagatga ttccctatct atataccatg aatgtaaaga cacatgagga aggtgcccca
     2341 ttaatcagtc caatctatta tttctatcca gagaatgatg agagctataa tgttccaaac
     2401 caatactttt ttggaacaga actgatggtg gctcccattg tagaaaagat ggatttgaca
     2461 ttccaatctg caaaagtaga tgtatggttc cctgaaggtg aatggtatga cttcttttca
     2521 gagaaaaaat acacaggtgg tgtgaagtta agtgtttata gggacatctc gactacgcct
     2581 gtgtttgcaa aaagtggtgc aatcattccc ttggttggtt ctgagatagg tatgggtgtt
     2641 gatttacctg aggttgtaga ttggtatgta ttcccaggga aacaacattc ttttgaaatg
     2701 cttgaagatc aaaatggtca aagatataaa acaagattat caatcgattg ggaaatggga
     2761 atggtagagt tagcattaca aggagattct agtatcgttc caagcaatag aaaacataga
     2821 attcatttta aaggaacgaa tgtgtctata attgaattgc caaataagaa tgatacagct
     2881 aaatttgaat ggaaagataa taaaaggaca tctttaaatg acgaagtttt tagactacta
     2941 aagacagctt ctcttccata tgaattaaaa gatagattgt taaatcaatt catcaatgcg
     3001 aaaaactctc atgacttaat gaatatcttg catcatcagg ataaggaatt gagagggcgt
     3061 ttgttggaaa tgatatttac tagccaaaac taattgatat aattagaaag tcatttgttt
     3121 agtgtatatg aagattctgt atggtattcg ttatcactgg tcacattgaa aatgtgaaat
     3181 aagcacctac aacatagact ttacaacact tcatgtttca tatcatgggg tgtttttcta
     3241 tttaagtcaa agccaatcaa aaaatctcac tattcatcag gttaaaggaa acctaatcat
     3301 agtgagattg tgaattatag tagagtttgt atcgcctctt caatttgttt tgggtctgtt
     3361 tttgaagaaa agcgttcaaa gacttgccca tcacgactaa tgagaaactt agcgaaattc
     3421 cattcgattc gttttcctag tgggccagat ttatggtctt ttaaccagac atagagaggg
     3481 tctgcttcct taccgttgac cttaatcttg gcaaaacgtg ggaaggtggt ttgaaaatgt
     3541 aggctacaga aggcgttgat ttcctctgcg ctgccgggtg cttgtcccat aaactgattg
     3601 caagggaaat ccaatatttc gaagccctga tcttgatagc gttcatagag ttcttgaagt
     3661 ccctggtact ggggcgttaa accacatcca gtagcagtgt tgacaatcaa gagaacttta
     3721 ccacgatagc tatccaaggg agttgcttgg ttgttttggt tcaaaacgga aaaatcataa
     3781 agtgaagtca tgagggcctc ttttcttttt ctacattgta acagttttta cttttttcgt
     3841 cgagtcggat ggaagatatt gttgaagaat aagccctcaa tatgatagaa accgtttaca
     3901 aataacttta aaaatggtat gatttaatta aaccggttta cttagttgct ataaatctct
     3961 aatttgagat ttagttccta ttgattttac aatatgttta ttggagtgta tacatgcaaa
     4021 caaaaacaaa gaagctcatt gtgagtttgt cttcacttgt tttatcagga tttttattaa
     4081 accattatat gacagttgga gcggaagaaa cgactacgaa taccattcag caaagccaga
     4141 aggaagttca gtatcagcaa agggatacaa aaaatttagt tgaaaatggt gattttggtc
     4201 agacggagga cggaagcagt ccgtggacag gaagcaaagc tcaggggtgg tcagcttggg
     4261 tagaccagaa gaatagttct gcagatgcct caattcgagt cattgaggct aaggatgggg
     4321 ctatcactat ctcaagccct gagaaattaa gggcagcggt tcaccgtatg gttcctattg
     4381 aagctaagaa aaagtataaa ctgcgtttca agattaaaac agatcataaa gtcgggattg
     4441 ccaaggttcg tatcattgag gaaagtggta aggacaagcg attgtggaat tctgcaacga
     4501 cgtcaggaac aaaggactgg cagaccattg aagcagacta tagcccgact ttagatgttg
     4561 ataaaatcaa gctggagtta ttctatgaaa caggaactgg gactgtttcc tttaaggata
     4621 ttgagctggt agaggtagca gaccagcttt ctgaggattc tcaaacagat aaacagcttg
     4681 aggaaaagat tgatttacca attggaaaaa aacatgtttt ttctcttgcg gactatactt
     4741 ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg aattttagaa cctcttaagg
     4801 aagggacaac caatgtcatt gtcagtaaag atggcaagga agtgaaaaag attcctttga
     4861 agattctagc ctctgttaag gatgcataca cagaccgttt ggatgactgg aatggcatca
     4921 tcgctgggaa tcaatactat gattctaaaa atgaacagat ggccaaatta aaccaggaat
     4981 tggaaggaaa ggtagctgat agcctatcta gtatttcaag tcaggcggac cgcacctatt
     5041 tgtgggaaaa attttcaaat tataaaatgt ctgcaaatct gactgccact tatcggaaat
     5101 tggaggagat ggccaagcaa gtgaccaatc cttcttctcg ttattatcaa gatgaaactg
     5161 tcgttcgaac agtcagggat tccatggaat ggatgcataa acatgtctac aatagtgaaa
     5221 agagcattgt tgggaactgg tgggattatg aaatcggtac acctcgtgcc atcaacaata
     5281 ccttgtctct gatgaaagaa tacttctctg atgaggaaat taaaaaatat acagatgtga
     5341 ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac gactgataac ccatttaagg
     5401 ctctaggtgg aaacttagtt gatatgggaa gagtaaaagt aatagctggt ttactgcgta
     5461 aggatgatca agaaatttct tctaccattc gctcgattga gcaagtgttc aagttggtag
     5521 accaaggtga aggtttttat caagatggat cctatatcga ccataccaat gttgcctata
     5581 cgggtgctta tgggaatgtt ttgattgatg gcctgtctca actgttgcca gtcattcaaa
     5641 agaccaagaa tccaatcgat aaagataaaa tgcaaaccat gtaccactgg attgataaat
     5701 cgtttgctcc tttgctggtg aatggagagc tgatggatat gagtcgtgga cgctcgatca
     5761 gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt actaagaggg attcaccgaa
     5821 tagcggatat gtctgaagga gaaaccaaac aacgtttgca gagtcttgtg aagaccattg
     5881 ttcaatcgga tagttattat gatgtcttta agaatttgaa gacttataag gatatcagtt
     5941 tgatgcaatc cttgttaagt gatgcaggag tcgcaagtgt tccaagaaca agttacctat
     6001 ctgcctttaa caagatggat aaaacagcca tgtacaatgc agagaaaggg tttggatttg
     6061 gcttgtcact cttttccagt cgtaccttga attacgaaca catgaacaag gaaaataaac
     6121 gtggttggta tacgagtgat gggatgttct atctttacaa tggcgatttg agtcactata
     6181 gcgatggcta ctggccaaca gttaatccat ataagatgcc tggtacaaca gagacggatg
     6241 ctaagagagc ggatagcgat acaggtaaag ttttaccgtc tgctttcgtt ggaacgagca
     6301 aactagatga tgccaatgcg acagcaacca tggatttcac caactggaat caaacattga
     6361 ctgctcataa gagctggttt atgctaaagg ataagatcgc ctttttagga agcaatatcc
     6421 aaaacacttc aacagatact gctgcaacta caattgacca gagaaaactg gaatcaagta
     6481 atccatataa agtctatgtc aatgataaag aagcctccct tacagaacaa gaaaaggatt
     6541 atcctgaaac ccaaagtgtc tttttagaat catccgattc gaaaaagaat attggttact
     6601 ttttctttaa gaagagttca atcagtatga gtaaggcttt gcaaaaggga gcctggaagg
     6661 atatcaatga aggacagtca gacaaggaag ttgaaaatga atttcttacg attagtcagg
     6721 ctcataagca aaatggagat tcttatggct atatgctcat ccctaacgtg ggtcgtgcca
     6781 ccttcaatca aatgataaaa gagttagaaa gtagcctcat cgaaaataac gaaacccttc
     6841 agtctgttta tgatgccaaa caaggagttt ggggcattgt gaaatatgat gattctgtct
     6901 ctactatttc caaccaattc caagttttga aacgtggagt ctataccatt cgaaaagaag
     6961 gggatgaata taagattgcc tactataatc ctgaaaccca ggaatcagct ccagatcagg
     7021 aagtctttaa aaagctagag caagcagctc agccacaagt acagaattca aaagaaaagg
     7081 aaaaatctga agaggaaaag aaccattcgg atcaaaagaa tctccctcag acaggagaag
     7141 gtcagtcaat cttggcaagt ctagggttct tgctacttgg ggcgttttat ttattccgta
     7201 gaggaaagaa caactaattt gttcatcatc tagatgaatt acatgaaact gtggaaaata
     7261 gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct tttaaaaaag ataagttgat
     7321 acttatcttt tttacttcat tatgtgacta tagcgttttg aaactagaat aggacgtcat
     7381 aactgctaaa agatttctat aaattcattt gattttccta atcaatttgt tcgtatccta
     7441 tttcactcca ctatattagt ccgtctcgct ccgtgaggtg cgagacaaaa caaccacccg
     7501 ctatgcgggt gcgcgtcgaa ggttatacca aaaaaactcc aaacgcgata caataaaggt
     7561 gttcaatcca cttgtaaagc gaaaggagaa aaaatatggc acaaaaggct catagtttat
     7621 ctcacacaaa gtggaggtgt tctatcacat tgtgttcacc cctaagtata gacgaaaagt
     7681 catctataat caatatagaa gtagtttagg cgaaatattt catcgcttgt gtagttataa
     7741 aggtgttgaa atgatcgagg gtcacttaat gccagaccat gtacacatgt tagtcagtat
     7801 tccactaagg ctaagtgttt cgagtttcat gggttattta aaaggcaaaa gtgcactcat
     7861 gatgtttgac aaacacgcca atctcaagta caagtttggg aatcggcatt tctgggcgga
     7921 aggttattat gtaagtccag tagggcttaa tgaagccaca attaagaaat atagtcaaga
     7981 ttaaagaaat tatccagtgg atgatttctt cacgagtatg aaaatttgag aactagtaaa
     8041 gtatgatata tagtaaaatg aaataagaac aggac
//
LOCUS       DAVKZB010000081         8061 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKZB010000081 DAVKZB010000000
VERSION     DAVKZB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.081"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            476..2581
                     /locus_tag="VXA80_001535"
     CDS             476..2581
                     /locus_tag="VXA80_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0354011.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMSQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            3033..3254
                     /locus_tag="VXA80_001536"
                     /pseudo
     CDS             3033..3254
                     /locus_tag="VXA80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(3540..4022)
                     /locus_tag="VXA80_001537"
     CDS             complement(3540..4022)
                     /locus_tag="VXA80_001537"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV0354012.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4117..5601)
                     /locus_tag="VXA80_001538"
     CDS             complement(4117..5601)
                     /locus_tag="VXA80_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV0354013.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPGGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            5754..7361
                     /locus_tag="VXA80_001539"
     CDS             5754..7361
                     /locus_tag="VXA80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV0354014.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIARLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            7521..7694
                     /locus_tag="VXA80_001540"
     CDS             7521..7694
                     /locus_tag="VXA80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV0354015.1"
                     /translation="MNIKKRVLSAGLTFASALLLATCGQSGSDTKTYSSTFGGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(7687..8061)
                     /locus_tag="VXA80_001541"
                     /pseudo
     CDS             complement(7687..8061)
                     /locus_tag="VXA80_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2444 a         1491 c         1846 g         2280 t
ORIGIN      
        1 aaaatattgt tagaaatcga tttgactgtc ctgattgatt tgtcctgttc ttatttcgtt
       61 ttaatataca gttttgagat ggttgataga actgacgaag tcagctcaaa acacggtttt
      121 gaggttgtag ataaggagaa gctgatgtgg tttgaagagg ttttcgaaga gtgtaaaaaa
      181 atattggtca gttaaagtca aaacccttga ccttttctga ccaatgtagt atattatgaa
      241 cgtaaggtaa atgaaagcct tactagaaca cttatttaaa gtaaaataga aagaggtggg
      301 tctatgtttc atctagaaat tttcagaagt aaagatggtc tactcctgct tgaaaaagaa
      361 aaaccagaaa tagtacgtag ggtagcggtt tagctagtct aaatttttta aaacaaaggt
      421 caaagatagt caatatcagt aatcataact aagtaaacaa aaagaggtaa agaatatgaa
      481 taacaacttt aataatttta ataacatgga tgatttattt aaccaattga tgggtggtat
      541 gcgaggatac agttctgaaa atcgccgtta cttaattaat gggcgcgaag tcacacctga
      601 ggaatttgct cactatcgta cgactggtca attaccagga aatgcagaaa ctgatgtgca
      661 aatgtcacaa caggcatcag gtatgaaaca agacggtgtc cttgcaaaac taggtcgaaa
      721 cttgacagca gaagcgcgtg agggtaagtt ggatcctgtt atcggacgaa acaaggaaat
      781 tcaagaaaca tctgaaatcc tctcacgccg caccaagaac aatcctgttt tggtcggaga
      841 tgcaggtgtt ggtaagacag cagttgtcga aggtctagcg caagccattg tgaacggaga
      901 tgttcctgct gctatcaaga acaaggaaat tatttctatt gatatctcag gtcttgaggc
      961 tggtactcaa taccgtggta gctttgaaga aaatgtccaa aacttagtca atgaagtgaa
     1021 agaagcaggg aatattatcc tcttctttga tgaaattcac caaattcttg gtgctggtag
     1081 cactggtgga gacagtggtt ctaaaggact tgcggatatt ctcaagccag ctctctctcg
     1141 tggagaattg acagtgattg gggcaacaac tcaagacgaa taccgtaaca ccatcttgaa
     1201 gaatgctgct cttgctcgtc gtttcaacga agtgaaggtc aatgctcctt cagcagagaa
     1261 tacttttaaa attcttcaag gcattcgtga cctctatcaa caacatcaca atgtcatctt
     1321 gccagacgaa gtcttgaaag cagcggtgga ttattctgtt caatacattc ctcaacgtag
     1381 cttgccagat aaggctattg accttgtcga tgtaacagct gctcacttgg cggctcaaca
     1441 tccagtaaca gatgtgcatg ctgttgaacg agaaatcgaa acggaaaaag acaagcaaga
     1501 aaaagcagtt gaagcagaag attttgaagc agctctaaac tataaaacac gcattgcaga
     1561 attggaaagg aaaatcgaaa accacacaga agatatgaaa gtgactgcaa gtgtcaacga
     1621 tgtggctgaa tctgtggaac gaatgacagg tatcccagta tcgcaaatgg gagcttcaga
     1681 tattgaacgt ttgaaagata tggctcatcg cttgcaagac aaggtgattg gtcaagataa
     1741 ggccgtagaa gttgtagctc gtgctatccg tcgtaaccgt gctggttttg atgaaggaaa
     1801 tcgcccaatc ggcaacttcc tctttgtagg gtctactggg gttggtaaga cggagcttgc
     1861 taagcaattg gcactcgata tgtttggaac ccaggatgcg attatccgtt tggatatgtc
     1921 tgaatacagt gaccgcacag ctgtttctaa gctaattggt acaacagcag gctatgtggg
     1981 ttatgatgac aatagcaata ccttaacaga acgtgttcgt cgcaatccat actctatcat
     2041 tctcttggat gaaattgaaa aggctgaccc tcaagttatt acccttctcc tccaagttct
     2101 agatgatggt cgtttgacag atggtcaagg aaatacagta aacttcaaga acactgtcat
     2161 tattgcgacc tcaaatgctg gatttggcta tgaagccaac ttgacagaag atgcggataa
     2221 accagaattg atggaccgtt tgaaaccctt cttccgtcca gaattcctca accgctttaa
     2281 tgcagtcatc gagttctcac acttgactaa ggaagacctt tctaagattg tagatttgat
     2341 gttggctgaa gttaaccaaa ccttggctaa gaaagacatt gacttggtag tcagtcaagc
     2401 ggctaaagat tatatcacag aagaaggtta cgacgaagtc atgggggttc gtcctctccg
     2461 tcgagtggtt gaacaagaaa ttcgtgataa ggtgacagac ttccacttgg atcatttaga
     2521 tgctaaacat ctggaagcag atatggaaga tggcgttttg gttattcgtg agaaagccta
     2581 agacagaatt ttgaggataa aaaagaagga gccagctgaa aaaaactggt tcctttctag
     2641 gtacgacggg catgtcgtgt agtaggttga aactagaata gtacacatct acttctaaaa
     2701 catttttaga aatcgatttg actgtcctga ttgatttgtc ctgttcttat ttcattttac
     2761 tatatatctg aggtgtaagt cctcggtggg tacctgctac cggtgctccc aatagtgatt
     2821 cccaagcctg actatcgtga ggtagcggat taaaacggtc tggggataga ccgttttaag
     2881 tctgacgctg gaaataagaa tcgtcagagg aagggatagc gaaatcgtgg ctctacgaat
     2941 aggaacgtga taataaggcg tatatagcgg ataaggggcc tcaaactcta aagttcaaaa
     3001 aggtagtcgt aacctatatg cgtaaatcac gagagcaact gaatttgtcc attcgaggat
     3061 ggataaacta tttctcattg ggaaatatga aaagtatagt caccagcata gatgagcgtt
     3121 tgcatactcg cctacgagtg attatctgga agcaatggaa gaagaaatcg agacgattat
     3181 ggggattgct taagttaggg gttcctaaat ggatagcaga taaggtatct ggctggggcg
     3241 accactatca attatagtaa aatgaaataa gaatagaaca aatcgatcag gatagtcaaa
     3301 tcgatttcta acaatgtttt agaagcagag gtgaactatt ctagtttcaa tccactatag
     3361 tagctcagaa gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa aaacgtggat
     3421 tggtttcgtg tttggattat taccttgaac gacatgcgtt aaaagttagt tgaaccgccg
     3481 tatgccgaac ggcacgtacg gtggtgtgag aggggctaga gattatcccc tactcgattt
     3541 taaatcacat gacgttcaaa ggcatcatct gaaatccctt gttccaagat gagttttgcc
     3601 cattctttag cagagaagag gctgtggtcc ttgtagtttc cgcaagattc gatggttgtc
     3661 cctgggacat cttcccaagt agtagtttca gcgatttcct tgagcgaatc cttgataaca
     3721 gctgcgattt tagcactggt gtgacgtccc cacataatca tgtggaagcc tgtgcggcaa
     3781 ccaaatggtg aacagtcaat catgccgtca atgcgggtac ggatgagttt ggctaagagg
     3841 tgctcgatag tgtgaaggcc ggcagtaggg atagagtctt cgtttggttg caccaagcga
     3901 atatcataat tggagatgat gtctcctttt ggtcctgttt cttccccaat caagcgaaca
     3961 tagggtgctt tgacaatggt gtggtcaagt tcaaaacttt cgacaataac ttcttttgac
     4021 atggtaaatc ctttcagttt tcttctctca ttatatcata aaggttgttc ctgagacaga
     4081 gagaaaacct ctccgaggct agagaggttg aaatctttac ttacgatata agcggtcgta
     4141 ttggtagtat gggtcaaagg ttacgttgat acccagttta cgaaggacat tcttgtcttc
     4201 atcagtcaag atgatggttg agtgggcttc gcttcctttg aggttgccga gttcttccat
     4261 agcgcgggca gcatcaggat tttctgtagc tgtgatagca agtgcaatca ggatttcatt
     4321 tgaatgaagg cgtggattgc ggctaccgag atgatcgatt ttaagacctt ggatcggctt
     4381 aacaacttca ggttcgatta gttttacttc tttagcgatg tcagctgatt ttttgatggc
     4441 gttgatcaag gcagcggctg taggaccaaa gagttctgag ttcttaccag tgacgatttc
     4501 cccatttggc aattcaaagg ctagggctgg tccaccagtt tcttctgctt tttggcgcgc
     4561 aacgacagca accttacggt ctgcaggtgt gataccgagg tcgttcatga gcaactcaat
     4621 tttcttgacg gcagcttcgc caactttttc agctttgaag tcaagaactg tttgatagta
     4681 acggcggatg atttcttgtt tagaagcttc gacagcggcc tcgtcatctg taatagcgaa
     4741 accaaccatg ttgacaccca tatctgtcgg tgaagcgtat ggtgattttc cgagaatacg
     4801 ttccaacatg cgtttgagca ctgggaagat ttcgatatca cggttgtagt tgacagtggt
     4861 ttctccatag gtttgaagat ggaaggggtc aatcatgttg acatcatcaa ggtcagctgt
     4921 ggcagcttca taagccaagt taactggatg atgaagggga agattccaaa cagggaaggt
     4981 ttcaaattta gcgtagccag atttgatgcc attgatttgg tcgtggtaca tattggacat
     5041 acacgttgcc aattttccag aaccgggtcc aggagcggtt acgacaatca agttgcgact
     5101 ggttttgatg tagtcgtttt tgcccatgcc tcctggggaa atgatgtgat ccatatccgt
     5161 aggatatcct ttgattggat aatgaagata agaatcaatt ccgtttttct caagttggtt
     5221 gcggaaggca tctgcagcgg gttggccagc gtattgtgta atgacaacgg aaccaacaaa
     5281 aatccctaat tcattgaatt tgtcaatcaa acgaagaact tcttggtcat aagaaatgcc
     5341 taagtcgcca cgtgctttgg aatgttcaat gttgctagca ttaatggcaa tcacaacctc
     5401 aacctgctct ttcaattctt gcaagagctt gattttgttg tcaggttcat aaccaggaag
     5461 gacacgagca gcgtggaaat cttctaacat tttaccgcca aactctaagt agagcttgcc
     5521 gtcaaattgg ttaatgcgct ccaaaatatg gtcgcgttgt aaattcaaat actgttcaga
     5581 actaaaagct tgttttttca tttttttacc tctggactct attataataa aaaattggaa
     5641 gttaggaaac tacggagcta aaaaagaaat taaaaagatt aagcaaacga ttgcacaaaa
     5701 ttttaaaaag tgctatcata gactatagat tattaaaata atgaggtaaa cagatgcaag
     5761 aaaaatggtg gcataatgcc gtagtctatc aagtctatcc aaagagtttt atggatagta
     5821 atggagatgg agttggtgat ttgccaggta ttaccagtaa gttggactat ctagctaagt
     5881 taggaatcac agcgatttgg ctttctcccg tttatgacag ccctatggat gataatggct
     5941 acgatattgc tgattatcaa gcgattgcgg ctatttttgg aaccatggag gacatggatc
     6001 agctgattgc agaagctaag aagcgtgata tccgtatcat catggacttg gtggtcaatc
     6061 atacctcgga tgagcatgct tggtttgtcg aagcctgtga aaatactgac agccctgagc
     6121 gagactacta tatctggcgc gatgaaccca atgacctaga ttctatcttt agtgggtctg
     6181 cttgggaata cgatgaaaag tcaggtcaat actatctcca ctttttcagc aagaaacagc
     6241 cggatctcaa ctgggaaaat gaaaaacttc gccagaaaat ttatgagatg atgaacttct
     6301 ggattgataa aggtattggt ggtttccgta tggatgttat tgacatgatt ggcaaaattc
     6361 ctgacgagaa ggtagtcaat aatggtccta tgctccatcc ctatctcaag gaaatgaatc
     6421 aggcgacctt tggagataag gatctcttga cagtagggga gacttgggga gcaacgccag
     6481 agattgccag gctctactct gatccaaagg ggcaagaatt gtctatggtc ttccagtttg
     6541 aacatatcgg tcttcagtat caggaaggtc agcctaaatg gcactatcaa aaagagctga
     6601 atatcgctaa gttaaaagaa atcttcaaca aatggcagac agagttagga gttgaggacg
     6661 gctggaattc cctcttctgg aacaaccatg acctccctcg tattgtctca atctggggaa
     6721 atgaccaaga ataccgcgaa aaatctgcca aagcctttgc aatcttgctt catcttatga
     6781 gaggaactcc ttatatctac caaggtgagg agattgggat gaccaactat ccgtttgaaa
     6841 cactggatca agtagaagat attgaatctc tcaactatgc gcgtgaggct cttgaaaaag
     6901 gtgttccgat tgaagaaatc atggacagta tccgtgttat tggacgtgac aatgcccgta
     6961 cccctatgca atgggacgag agcaaaaacg ctggtttctc aacaggtcaa ccttggttgg
     7021 cggttaatcc aaattacgag atgatcaatg tccaagaagc gctggcaaat ccagattcta
     7081 ttttctatac ctatcagaaa ctggtccaaa ttcgcaagga gaatagctgg ctagttcgag
     7141 ctgactttga attgcttgat acggctgata aggtctttgc ttatatacgt aaggatggcg
     7201 accgtcgctt cctagttgtg gctaacttgt ccaatgaaga gcaagacttg acagtagaag
     7261 gaaaagtcaa atctgtcttg attgaaaaca ctgcggctaa agaagtactt gaaaaacagg
     7321 tcttggctcc atgggatgct ttctgtgtgg aattactata aatatttttt gcagaaaaat
     7381 ttaaaattga aatcatataa aaacaaggga ggactgtata aaagacagaa atcctttgtt
     7441 ttttataacc aaggtttata aactttcatt ctcgaaattc aattaacttt acaaattccc
     7501 actatttaag gagaaagaag atgaacataa agaagcgagt ccttagtgca ggcctgactt
     7561 ttgcatctgc tttgctttta gctacttgcg gccaatcagg ttcagataca aaaacttact
     7621 catcaacctt tggtggaaat ccaactacat ttaactatct attagactat tacgctgata
     7681 acatagtcaa ttgaaacaag aacaggacaa aagagcctca taaaaggtat tgcaacttgg
     7741 taataccttt tacgaggctt tttgatatga gcccatgttt tctcaatagg attgtactca
     7801 ggtgagtagg gaggaagagg taaaagttta tacccaaact cttcacacaa gagttctaac
     7861 ttacccattc tatggaatct tgcattatcc ataataataa ccgatggtgt ggttaatgtt
     7921 ggtaagagaa acttctgaaa ccaagcttca aaaaagtcgc tcgtcatcat ctcttcgtaa
     7981 gtcattggag cgattaactc accattcatt tgttagacct gcaaccaaag aaattctctg
     8041 atatcttctt ccagatactt t
//
LOCUS       DAVKZB010000082         7922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKZB010000082 DAVKZB010000000
VERSION     DAVKZB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.082"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            125..706
                     /locus_tag="VXA80_001542"
     CDS             125..706
                     /locus_tag="VXA80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0354016.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLYVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            703..1980
                     /locus_tag="VXA80_001543"
     CDS             703..1980
                     /locus_tag="VXA80_001543"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV0354017.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            1998..2660
                     /gene="deoC"
                     /locus_tag="VXA80_001544"
     CDS             1998..2660
                     /gene="deoC"
                     /locus_tag="VXA80_001544"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV0354018.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            2647..3036
                     /locus_tag="VXA80_001545"
     CDS             2647..3036
                     /locus_tag="VXA80_001545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0354019.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3126..4178
                     /locus_tag="VXA80_001546"
     CDS             3126..4178
                     /locus_tag="VXA80_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV0354020.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4322..5857
                     /locus_tag="VXA80_001547"
     CDS             4322..5857
                     /locus_tag="VXA80_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354021.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            5850..6908
                     /locus_tag="VXA80_001548"
     CDS             5850..6908
                     /locus_tag="VXA80_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354022.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGSVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            6911..7867
                     /locus_tag="VXA80_001549"
     CDS             6911..7867
                     /locus_tag="VXA80_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354023.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2231 a         1437 c         1906 g         2348 t
ORIGIN      
        1 tgttgaaaga gattgaagaa aatggcttcg tgacttgctt tcttcggtct ttttcgtttt
       61 attatcaatt tgtaaacgat ttacaatttg agtcaaatta tggtaaaata gtgtcatgag
      121 taaaatgtat tatgcagaaa atcctgacgc tgctcacgac attcatgagt tgagagtgga
      181 cttgttggga caaaaaatga cttttttgac agatgcgggt gtttttagca agaaaatggt
      241 tgactttgga agtcaactct tgctcaagtg tctgtatgtc aaccaaggag agacagtcct
      301 agatgtaggc tgtggttatg gtccattagg tttgtccttg gtcaaggttt acggagttca
      361 ggcgaccatg gttgatatta acactcgtgc cttggattta gctcggagaa atgctgaaaa
      421 aaataatgca aaagcgacga ttttccagtc caacatctat gaacaagttg aaggacattt
      481 tgatcatgtc atttccaatc ctcctatccg tgcgggcaag caagtcgttc atgaaatcat
      541 tgagaaaagt aaagatttct tggaaactgg tggggatttg acaatcgtca tccagaaaaa
      601 acaaggggct ccaagtgcta agtccaagat ggaggatgtg tttggtaatt gtgaaatcct
      661 taaaaaagac aaaggatact atatccttag aagtgtgaaa gaatgagagc agttgattta
      721 atacaaaaaa aacgagacgg tcaagaactg acttcaagtg aaattgaatg gctggtagaa
      781 ggctatgtgt caggaactgt tcctgattat cagatgtctg cctttgctat ggctgtttat
      841 tttaaaggaa tgacgactcg tgaaatttct gatttaacga tgaatatggt taagactggg
      901 caagagtttg acctgtcagc cattgatggt gtgaaagttg acaagcattc tactggtggt
      961 gtcggcgata aggtaacctt gattttagct cctcttgttg ctagcttcgg tgtgcctgta
     1021 gcaaaaatga gtggtcgtgg tcttggccat acaggcggaa caattgataa attggagtcc
     1081 attaagggct atcaagtaga acgtagtcaa gaggatttta ttcgtcaggt tcaggatatt
     1141 ggagtatctg tcattgggca atcagaccag ctggtcaaag cagataagct tctctatgcc
     1201 cttcgtgatg tgaccgcaac tgtcgacacg attcctttga ttgcgagttc ggtgatgagc
     1261 aagaaaattg cggcaggggc ggatgctatt ttgctagacg tgactgtcgg tgagggtgcc
     1321 ttcatgaaga cggttgatga ggcgcgtgag ttggctcaaa ctatggtaga tcttggtaag
     1381 gttgttggac ggaagacagt agcagtgatt acggatatga gccagccttt gggacgagcg
     1441 attggaaatc gtctggaaat ccttgaagca ttggagattt tgcaaggtca aggacgtcag
     1501 gatattaccc actttatttg tgaattggct caaattatgc ttggtttggc aaatgttaac
     1561 aaaactgttg aagaagttcg ccaacatctt gaaaatggtc aagcactggc taagtttgag
     1621 gaaatggtgc aagcccaagg tggagacttg gaagacctct atcgtcctgt aaatgtagct
     1681 catgtggtgg aaatccctgc tcaggaaacg ggtgtcattt cagctcttcc agctatggac
     1741 tttggtcttt atgccatgag gctgggtgct ggtcgtgcag ttaagtctga tgtcttggac
     1801 tatgaaacag gaattgtttt tgaaaagaag attggagact ccgttcaaaa gggtgaaatt
     1861 gttgcaaaag tatacacaaa tgaaaaaatt ccacctcaac tagttacaga tttccaaaaa
     1921 tgtgttaaaa taagtgatga agtgaaaaaa atccgtgaaa ttgtagaaat catctcctaa
     1981 atggagggaa aaccgaaatg aaattaaata aatatataga tcatacgctt ttaaaacaag
     2041 atgcaaagaa aaaacaaatt gatagtttgt tgtctgaggc tagagagtat gactttgcca
     2101 gtgtttgcgt taatccgacc tgggttgaac atgctaaaaa aggacttgaa ggcacagatg
     2161 ttaaggtttg cacagtagta ggtttccctt tgggagcaac aacttcagcc gtgaaagcat
     2221 ttgagacaaa agaagctatc caaaatggtg cagatgagat tgatatggtg atcaatgttg
     2281 gagctctcaa atcaggtaat ttagccttgg ttgagtcaga tattcgcgca gtagtggaag
     2341 caagtggtga taagttagtg aaagtcatta ttgaagcttg ccttctgaca gaccaagaaa
     2401 aaattgttgt ttgccaattg gcccaaaaag ctggggctga ctttgttaaa acatctactg
     2461 gcttttcaac tggtggtgct acgatagcag atgttagatt aatgcgtgaa acagttggat
     2521 ctgatatggg agtcaaggct gctggtggag ctcgttctta tgcagatgct cttgcctttg
     2581 tcgaagcagg tgcgacccgt atcggaacgt cagctggggt agctatttta aaaggagaat
     2641 tggcagatgg cgactactga gttgattgaa ctggcaattg aaaccagcaa acatgcctat
     2701 gtcccctatt ctcactttcc tatcggagca gttttagtag ccaaagacgg aagtgtttac
     2761 acgggagtga atatcgaaaa tgctagctat cctttgacca attgcggtga gagaacagct
     2821 gtttttaagg ctatatctga aggccaaaga gaattttcag aattgattgt ctatggtcaa
     2881 actgaaaaac cgatctcgcc atgtggtgct tgtcgccaag taatggtcga attttttgaa
     2941 caagatttaa aagtgacctt agtcgcaaaa gataaatcga cggtcgagat gacggtcggg
     3001 gaattacttc catactcttt tacagactta aactagtctg agtcgctctc tgagtggcac
     3061 ggtccttgtg gccaaccaat ccatacttgc aacatcgttg cacatcttat ttaggaggtt
     3121 cagtaatgaa caagaaacaa tggctaggcc ttggtctagt tgcagtggca gcagttggac
     3181 ttgctgcatg tggtaaccgc tcttctcgta acgcagcttc atcttctgat gtgaagacaa
     3241 aagcagcaat cgtcactgat actggtggtg ttgatgacaa atcattcaac caatcagctt
     3301 gggaaggttt gcaggcttgg ggtaaagaac acaatctttc aaaagataac ggtttcactt
     3361 acttccaatc aacaagtgaa gctgactacg ctaacaactt gcaacaagcg gctggaagtt
     3421 acaacctaat ctttggtgtt ggttttgccc ttcataatgc agttgaagaa gcagctaaag
     3481 aacacactga cttgaactat gtcttgattg atgatgtgat taaagaccaa aagaatgttg
     3541 cgagcgtaac tttcgctgat aatgagtcag gttaccttgc aggtgtggct gcagcaaaaa
     3601 caactaagac aaaacaagtt ggttttgtag gtggtatcga atctgaagtt atctctcgtt
     3661 ttgaagcagg attcaaggct ggtgttgcgt cagtagaccc atctatcaaa gtccaagttg
     3721 actacgctgg ttcatttggt gatgcagcta aaggtaaaac aattgcagcc gcacaatacg
     3781 cagccggtgc agatattgtt tatcaagtag ctggtggtac aggtgcaggt gtctttgcag
     3841 aggcaaaatc tctcaacgaa agccgtcctg aaaatgaaaa agtttgggtt atcggtgttg
     3901 atcgtgacca agaagcagaa ggtaaataca cttctaaaga tggcaaagaa tcaaactttg
     3961 ttcttgtatc tactttgaaa caagttggta caactgtaaa agatatttct aacaaggcag
     4021 aaaaaggaga attccctggc ggtcaagtga tcgtttactc attgaaggat aaaggggttg
     4081 acttggcagt aacaaacctt tcagaagaag gtaaaaaagc tgtcgaagat gcaaaagcta
     4141 aaatccttga tggaagcgta aaagttcctg aaaaataatg gatggaagtc atttcttagg
     4201 aagcggccaa gggccgcttt tttaagagtt gagacaaagt cattttgtct cagtaaagct
     4261 tgctactaga caaactagca agttttattg aaataaaata agactctgaa aggaagagca
     4321 catggcacac gaaaatgtca ttgagatgcg tgatattacc aaggtgtttg gtggatttgt
     4381 tgctaacgac aaaatcaact tgcacctacg aaaaggtgaa attcatgcac ttttaggaga
     4441 aaatggggct ggtaagtcca cgctaatgaa catgttagca ggccttcttg aaccaactag
     4501 tggtgaaatc gcggtcaacg gtcaagttgt caatctcgac tccccatcta aagcagctag
     4561 cttgggaatc gggatggttc accagcactt tatgttggtt gaagccttca cagtggctga
     4621 aaacatcatt ttaggtagtg aattgactaa aaatggtgtg ctagatatcg ctggagctag
     4681 caaagaaatc aaggctcttt ctgaacgtta tggcttagct gttgaccctt ctgccaaggt
     4741 agcagatatc tcagttggag cccaacaacg tgtagaaatt ttaaaaacac tttatagggg
     4801 ggctgatatc cttatctttg acgaaccaac ggctgttttg actccatcag aaattgatga
     4861 gttgatggct attatgaaaa atcttgtcaa agaaggaaaa tcaattatct tgattaccca
     4921 caaattggat gaaattcgcg cagtttctga ccgtgttaca gttatccgtc gtgggaaatc
     4981 aattgaaacc gttgaaattg caggggctac caatgctgat ttggcggaaa tgatggtagg
     5041 acgttctgtt tcctttaaaa cagagaagca agcctctaaa ccaaaagaag tggttttgtc
     5101 tatcaaagat ttggtggtca atgaaaaccg tggtgttcca gctgttaaaa atctgtcctt
     5161 ggatgttcgt gctggagaga ttgttggtat tgcggggatt gatggaaatg gtcagtctga
     5221 actgattcaa gccattacag gtcttcgtaa ggttgaatct ggtagcattg agctaaaagg
     5281 agattcaatt gtaggcttgc acccacgtca gattacagaa ctaagtgttg ggcacgttcc
     5341 agaagaccgt caccgtgatg gcttgatttt ggaaatgatg atatctgaaa atattgccct
     5401 tcaaacctac tataaagaac cacatagtaa aaatggaatt ttgaattatt caaatattac
     5461 ttcttatgct aaaaagctga tggaagagtt tgatgttcgc gctgccagtg aattagttcc
     5521 tgcagctgca ctctcaggag gaaatcaaca aaaagcaatt attgctcgtg aaattgatcg
     5581 agatcctgat ctccttatcg ttagccagcc aactcgtggg ttggatgtcg gtgccattga
     5641 gtatatccac aaacgcttga ttgaagagcg tgataatggc aaggctgtcc ttgttgtcag
     5701 ctttgaattg gatgagattt taaacgtctc agaccgtatt gccgttatcc acgatggtaa
     5761 gattcaaggt attgtatcac cagaaacaac caataaacaa ggacttggtg tcttgatggc
     5821 tggtggaaac ttgggaaagg agaagagtga tgtctaaaaa attacaacaa atttcggttc
     5881 ccttgatttc tgtattccta ggaattttac tcggagccat tgtcatgtgg atcttcggtt
     5941 atgatgctat ttggggctac gaagaattgt tctatacagc ctttggcagt ctgcgtggga
     6001 ttggagaaat cttccgtgct atgggtcctc tggtcttgat tggtcttggt tttgcggttg
     6061 ccagtcgagc tggtttcttt aacgtcggac ttcctggtca ggctttggca ggttggattc
     6121 tcagtggttg gtttgccctg tcgcatccag atatgccccg tcccttgatg attctagcaa
     6181 ccatcgtgat tgccttgatt gctggtggga ttgtcggagc gattccaggt attcttaggg
     6241 cctatctagg gacgtcagag gttattgtaa ccatcatgat gaactacatt gtcttgtatg
     6301 tagggaatgc ctttatccat gctttcccta aagacttcat gcaaagtaca gattcgacca
     6361 ttcgtgttgg ggctaatgca acctatcaga caccttggtt ggctgagttg actggtaact
     6421 cacggatgaa tattggtatt ttctttgcca tcattgccgt tgcagttatt tggttcatgc
     6481 tcaagaaaac aactcttggt tttgaaatcc gtgcagttgg tcttaatcca catgcttcag
     6541 aatatgctgg tatttctgcc aagcggacta ttatcctatc tatgattatt tcaggtgcct
     6601 tggcaggtct tggtggatct gttgaaggtt tgggaacctt ccagaacgtc tatgttcaag
     6661 gttcgtcatt agctatcgga tttaacggaa tggcggttag tttgcttgcg gccaactcac
     6721 caattggtat actctttgca gccttcctat ttggcgttct ccaagttggg gctcctggta
     6781 tgaatgcggc gcaggtacca tctgagcttg tcagcattgt aacagcgtct attatcttct
     6841 ttgtcagtgt tcattacctt atcgaacgct ttgtcaaacc gaaaaaacaa gttaaaggag
     6901 gtaagtaaag atgtctatta taaccttgct ccccctcttg gtgtcttcta tgctgattta
     6961 ctcagcacct ctcatcttta caagtatcgg tggtgttttc tctgaacgtg gtggtgtggt
     7021 aaacgttggc cttgaaggaa ttatggttat gggtgccttt tctggagttg tctttaacct
     7081 tgaatttgca gaacaatttg gagcagcaac tccatggcta tccttgcttg tagcaggatt
     7141 ggttggtagt gttttctcta tcatccacgc agcagcgacg gttcatttcc gtgcagacca
     7201 tgttgtcagc ggtacggtat tgaacttgat ggcgcctgcc ttggctgttt tcttagttaa
     7261 agttctttat aacaaaggac aaaccgacaa cctaagtcaa acttttggac gctttgattt
     7321 cccagtcttg gcaaatatcc cagtgattgg tgatatcttc ttcaagtcaa ctagtctact
     7381 tggttatctg gcgattgcct tctcattcct tgcttggttt attctcttta agactcaatt
     7441 tggtcttcgt ctccgctctg tcggtgaaca tcctcaagca gcggatacct tgggaatcaa
     7501 cgtctacaag atgagatatt tagggattat tatttcaggt tttctaggtg gaattggcgg
     7561 agcgatttat gcgcaatcaa tctcagttaa cttctcagtg acaactattg ttggacctgg
     7621 atttatcgcc cttgctgcga tgatctttgg gaaatggaat ccaatcggtg ctatgctttc
     7681 tagtctcttc tttggacttt cacaaagttt ggctgttatc ggttctcaat tgccgttcct
     7741 acaaggagtg ccagcggttt atcttcaaat tgcaccttat gttttgacaa ttcttgtctt
     7801 agcagccttc tttggaaaag cagtcgcacc aaaagcagat ggtatcaact acatcaaatc
     7861 aaaataagca tacaaaaaaa cgtcagtcca atgactgaac tgccccccaa aagttagaca
     7921 ga
//
LOCUS       DAVKZB010000083         7882 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKZB010000083 DAVKZB010000000
VERSION     DAVKZB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.083"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            442..1803
                     /gene="trpE"
                     /locus_tag="VXA80_001550"
     CDS             442..1803
                     /gene="trpE"
                     /locus_tag="VXA80_001550"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV0354024.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKSHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYLYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGAMDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKSTLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1800..2366
                     /locus_tag="VXA80_001551"
     CDS             1800..2366
                     /locus_tag="VXA80_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV0354025.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2377..3381
                     /gene="trpD"
                     /locus_tag="VXA80_001552"
     CDS             2377..3381
                     /gene="trpD"
                     /locus_tag="VXA80_001552"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV0354026.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3378..4145
                     /gene="trpC"
                     /locus_tag="VXA80_001553"
     CDS             3378..4145
                     /gene="trpC"
                     /locus_tag="VXA80_001553"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV0354027.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4132..4731
                     /locus_tag="VXA80_001554"
     CDS             4132..4731
                     /locus_tag="VXA80_001554"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV0354028.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            4709..5932
                     /gene="trpB"
                     /locus_tag="VXA80_001555"
     CDS             4709..5932
                     /gene="trpB"
                     /locus_tag="VXA80_001555"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV0354029.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5925..6701
                     /locus_tag="VXA80_001556"
     CDS             5925..6701
                     /locus_tag="VXA80_001556"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV0354030.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(6673..7332)
                     /locus_tag="VXA80_001557"
     CDS             complement(6673..7332)
                     /locus_tag="VXA80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV0354031.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLKKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            7402..7848
                     /locus_tag="VXA80_001558"
                     /pseudo
     CDS             7402..7848
                     /locus_tag="VXA80_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         2342 a         1570 c         1955 g         2015 t
ORIGIN      
        1 tacctacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataaaggtgt
       61 accgagatga acatctcttc atcaatgtct ctggtcgtgg aggcaaggac ctagctgcga
      121 ttgcagatta ctagaagcta aaaaataata gatggaaaaa ttacagttct ttctctcaca
      181 gagagcttgt ggttgctgaa aacaagcaga gaaagctgta aagttgggtt catctgaaac
      241 gagagaagaa aacatcattc tcaagggagt gccctttatc gcacagcctg ttacaggaga
      301 tatctgagac aggaatgaga gataggagaa atcctataat tgaggtggca ccgcgaattt
      361 cgtcctcacg caagttattt tgcgtgggga tttttcatat agtcaccacg gactagaagt
      421 agaactgaaa gggaaattac aatggaacga atcattcatg gagatgtctt atcaccaatc
      481 ttggcttata tgcgcttaaa ggggcaacac aaggttatct tagagagtat tccgagagac
      541 aaggaaacag ctcgtttttc tatcttagcc tataatcctg tttttgagat taagtttgaa
      601 aatggagttc tttatcaaaa tggtcaagtg attgatcggg atcccttgga tttcctttat
      661 gaagtgattc ataagagtca gcaccactca gagctacctt ttggtggtgg agccattggt
      721 tttgtgggtt acgatatgat ttcgctttat gaagaaattg gtcaaatccc tgaggataca
      781 attgggacgc cagacatgca tttctttgtc tatgagagct atatggtctt tgaccacaag
      841 aaggagaaga tacatgtcat agaggatgca ctttatagtg agcgcagtca agaagccttg
      901 gaaaaatcct tgaaccaagt gcttgaggaa ttacgcattc ctgctccaaa tgaatttgaa
      961 gacttggatc tatctccgtt agacttcaaa tcccatatcg ctcctcagaa gtttgaggaa
     1021 atggtggaaa ccgctcgcga cttgattcgt aatggcgata tgttccaatg tgtactcagt
     1081 cagcgcttct cagcagaagt tactggaaat ccatttgact tctacagaaa tctccgcgtg
     1141 accaatccat ccaattacct ctatttatat gactttgggg attatcaaat catcggtgcc
     1201 agtccagaaa gtttggtttc tgtcaaaaat ggcatcgtga caaccaatcc gattgcaggg
     1261 acgcgaccaa gaggggctat ggatgaagaa gacaaggcct tggcgactga cctcctctct
     1321 gatgagaagg aaacagcaga acatcgaatg ttggtagact tggggcgtaa cgatattggc
     1381 cgcatctctg aaacggccag tgtccaagtc actaagtata tggaagtgga gctcttccgt
     1441 tatgttatgc atctgaccag cgtggtcaag gggcgtttgc ttccagaact cactgctatg
     1501 gatgccttga aatcaacgct tccagctgga accgtttctg gagcaccaaa aattcgagct
     1561 atgagacgca tctatgaact ggaaacggaa aaacggggcg tatatgcagg agcaatcggc
     1621 tacttgtctg cgacgggtga tatggatttg gccattgcca tccgaaccat gattctcaaa
     1681 aatcaaagag cctatgtgca ggctggggca gggattgtct acgactctat cgcccaaaac
     1741 gaataccaag aaaccattaa caaagcaaaa tctatgacta gaattggaga actaagacca
     1801 tgattttatt gattgacaac tatgattctt ttacctataa cttggcccaa tacattggga
     1861 attttgcaga agttcaggtt ttgagaaatg atgattccaa gctgtatgaa gaagctgaaa
     1921 aagcagatgg tctggtcttt tctcctggtc ctggttggcc agttgatgct ggaaagatgg
     1981 aagacatgat tcgtgatttt gcaggcaaga agccgattct agggatttgt ttgggccacc
     2041 aagccattgc agaagtcttt ggtggtaagc taggtttggc tccaaaagtc atgcatggga
     2101 aacagagcaa tatcaacttt gaagcgccat ctgttttgta tcaaggtatt gaggatggcc
     2161 gtgcggtcat gcgttatcac agtattttga ttgaggaaat gccagaagac tttgaagtga
     2221 cagctcgttc gactgatgac caagccatca tggggattca acataaaaac ctaccgattt
     2281 atggcttcca gtaccatcca gagagcattg gaacgccaga cggcttgtct tctattcgga
     2341 attttatcga gaaggttgta aagtgaggaa actaggatga aagagattat tgaaaaacta
     2401 gcaaaatttg aaaatttatc aggtgtggaa atgacggatg tcattgagcg tatcgtaact
     2461 gggcgtgtaa ctgaagcgca gattgcttct ctcctcttag ctcttaagat gaagggggaa
     2521 acacctgaag aacgcacagc cattgcccaa gtcatgagag gacatgccca gcatattcca
     2581 actgagattc atgatgctat ggacaactgt ggtacaggtg gggacaagtc tttcagtttt
     2641 aatatttcca caactgcagc ctttgtcttg gctggtggcg gtattcacat ggccaagcat
     2701 ggtaaccgct cgatttcttc taaatctggt tccgcagatg tcctcgaagc cttgggaatc
     2761 aatcttgacc tcaaaccagc tgaactaggt aaggtctttg ataaaactgg aatcgtcttt
     2821 ctcttcgcta aaaatatgca cccagctatg aaatacatca tgccagctcg tttggaattg
     2881 ggaattccaa cgattatgaa cttgactggt ccactgattc atccaatggc tttagaaaca
     2941 cagcttcttg gaattagtcg tccagaactc ctagaaagta cagctcaggt tttgaaaaat
     3001 atgggtcgca aacgtgccat cgtggttgct ggaccagaag ggttggatga agctggcttg
     3061 aacggaacaa ccaagattgc acttcttgaa aatggcgaaa tcagcttgtc aagctttact
     3121 ccagaggatt tgggaatgga aggctatgct atggaagata ttcgtggtgg gaatgctcag
     3181 gaaaatgcag aaattttgct tagcgttctg aaaaacgaag caagtccatt cttggaaacg
     3241 acagtcttga atgctggtct tggtttctat gctaatggta agattgatag catcaaggaa
     3301 ggagttgcct tggcccgtca agtgattgct agaggcaagg cccttgaaaa actcagactg
     3361 ttacaggagt accaaaaatg agtcaggaat ttttagcacg aatcttagag cagaaggtgc
     3421 gtgaggtgga gcagatgaag ctggagcaaa tccagcctct gcgccagacc tatcgcttgg
     3481 cagaattttt gaagaatcat caggaccgct tgcaggtaat cgctgaggtc aagaaagcta
     3541 gccctagttt gggagatatc aatctcgatg tggatattgt gcaacaggcc cagacttatg
     3601 aagaaaacgg agcagtgatg atttcggtgt tgacagatga ggttttcttt aaagggcatt
     3661 tggattatct acgggaaatt tccagtcagg tagagattcc gacgctcaac aaagacttta
     3721 tcatcgatga aaagcaaatc atccgcgctc gcaatgcagg tgcgacagtt atcttgctca
     3781 ttgtggcagc cttgtccgaa gaacgcctca aggaactgta tgactacgcg acagagcttg
     3841 gtctggaagt cttagtggag actcacaatc tagctgaact agaggtagcc cacagacttg
     3901 gtgctgagat tatcggggtc aacaaccgca acttgactac ctttgaggtc gacttgcaga
     3961 ccagtgtaga tttagcccct tactttgagg aaggtcgcta ttacatttct gaatctgcca
     4021 ttttcacagg gcaggatgcg gaacgactag ccccatactt taacggaatt ttggtaggga
     4081 cagctcttat gcaggcagaa aatgtggccc agagaatcaa ggagttgcag attgacaaag
     4141 gttaaaattt gtggactatc gaccaaagaa gcggtggaaa cagccgtttc agcaggagcc
     4201 gactatatcg gttttgtctt tgcacctagt aaaagacagg tgactttaga agaggcagct
     4261 gagttggcaa agcttattcc tgcagatgtc aaaaaggttg gtgtatttgt ttcaccaagt
     4321 cgggcagaac tgctggaagc gattgacaaa gttggcttgg acttggttca agttcacggt
     4381 caggtggcag atgatttatt tgagaatttg ccttgtgcta gcattcaggc tgtgcaggta
     4441 gatggagagg gtcatgtgcc caattctcag gcagactatc tactctttga tgcccctgtg
     4501 gctgggagtg gccagccctt tgattggggt caactggata cgactggact agcacagccc
     4561 ttctttatcg caggtggcct taatgaagat aatgtagtaa aagcaattca acactttact
     4621 ccctatgcag tagatgtatc aagcggagtg gagacagatg gacaaaaaga tcatgaaaag
     4681 attagaagat ttatagagag ggtaaagaat ggcatatcaa gaaccaaata aagatggatt
     4741 ttacggaaaa tttggcggac gttttgtccc agaaacattg atgacagcag ttttggagtt
     4801 ggagaaggcc taccgtgaaa gtcaggcaga cccaagtttc caagaggaat taaaccagct
     4861 tttgcgccag tatgtgggac gtgaaactcc cctttactac gcaaaaaact tgacccagca
     4921 tatcggcgga gccaagattt atctcaaacg ggaagacctt aaccatacag gagcccacaa
     4981 gattaacaat gccttaggac aagtttggct tgccaaacgc atgggtaaaa agaaaattat
     5041 cgcagaaact ggtgctggtc agcacggtgt ggcaactgca actgctgcgg ccctctttaa
     5101 catggaatgt accatctaca tgggtgagga agatgtcaaa cgccaagccc tcaatgtctt
     5161 ccgtatggag cttttgggag ctaaggtcga ggccgtgaca gatggttcgc gcgtgctcaa
     5221 ggatgcggtc aatgcagccc ttcgttcatg ggtggctaat atcgacgata cccactatat
     5281 ccttggttct gccttggggc ctcatccatt tccagaaatc gttcgtgact tccaaagtgt
     5341 catcggtcga gaggctaaac aacagtaccg tgacttgaca ggtcgagatc tgccagatgc
     5401 cctagtagcc tgtgttggtg gtgggtcgaa tgccatcggt ctcttccatc cctttgtaga
     5461 agatgagtca gtagccatgt atggagctga agcggctgga tttggtgtgg atacggagca
     5521 ccacgcagct accttgacca agggtcgtcc aggtgtcctt cacggttccc tcatggatgt
     5581 gctccaagat gcccatggtc aaattcttga agccttctct atctcagcag gtttggacta
     5641 tcctggtatc ggtccagagc attctcacta ccacgatatt aaacgtgcca gctatgtccc
     5701 tgtgacagac gaagaagcct tggaaggatt ccaactcttg tctcgtgtgg aagggattat
     5761 cccagctttg gaatctagcc atgctatcgc ctttgcggtg aaattggcca aagaacttgg
     5821 accagaaaag tctatgattg tatgcctatc aggtcgtggg gacaaggatg tggttcaagt
     5881 aaaagaccgc ttggaagcag atgcagcaaa gaagggagaa gctcatgcct aagacactaa
     5941 caaaaaaatt aaatgctatt aaagcagctg gaaaaggaat ttttgttccc tatatcatgg
     6001 ctggtgacca cgaaaaaggt ttggatggtc ttgctgaaac aattcacttt ttagaagatt
     6061 tgggtgtctc tgcaattgaa gtgggtattc ccttttcaga ccctgttgca gatggacctg
     6121 ttatcgaaga agctggcttg cgcagtttag cccacgggac ctctacccag gctttagttg
     6181 aaaccttgaa aaccattgaa acagagattc cactggtcat catgacctac ttcaaccccc
     6241 tctttcagta cggtgtggag aactttgtca aagatctggc tgatacagct gtgaagggct
     6301 tgattatccc agacctgcct catgaacatg ccaactttgt agaaccattt ttggcagata
     6361 cagatatcgc cttgattcct ttagtaagct tgaccacagg aattgagcgc cagaaagagt
     6421 tgattgaagg ggcggaggga ttcgtctatg ccgttgccat caatggggta acagggaaat
     6481 caggcaatta ccgtgcagat ttggacaagc acttggcgca acttcatcaa gtggccgaca
     6541 tcccagtctt gacaggtttt ggtgtatcta gtcaagccga tctggaacgc ttcaatgcgg
     6601 tgtcagatgg tgttatcgtc ggttcgaaaa tagtaaaagc tctccaccaa ggagagccga
     6661 ttcaggactt tatcaaacaa gcagtagctt accaaaaata attaaacaag tagcaagtaa
     6721 gaggaaaggc acgaaaggaa gtctttcctt tttctttttc aggagaaagg ccaggatacc
     6781 cgtcgcagaa gcaaactgaa tcaagatcag taactccgtt acgctaaaga cgagagcaca
     6841 agaagctaaa aagaggaaat ctcctgcgcc catgcggata tcgataaaat gagccaaaat
     6901 tccaagagca aggaaggaga ccatgaccag attccagcca gaggaagcta ttaggattag
     6961 gtggaaagtc atccagacca gtaagggata ttcctgatgg tgaaagtcgt agatacccaa
     7021 ggtcaaacca gcggtgatta ggacgacttg ccccaaggaa agcaattccc aagagtaaag
     7081 cagaaagagg agtcctaagc ctagttcaaa gagggcatac cagacaggat agcgaacttt
     7141 gcagtagcga cagcgaaagc gattgaagac ctgtgagaga atcggaatca aatctaaggg
     7201 acgcaagcga gtctgacagg aatcgcagtg actggctgaa ctgataatgg attgctctgg
     7261 aaaacggtca atgaccaaac caagaaagga agcgagaatg ctcccgacga gaaaaaaata
     7321 aaaatcaatc atacttatct attcgtaaaa aatgggagaa atagtataat ggagaaacat
     7381 agataagatg gtgaggaaaa gatgacaatt cgttttgaag aaaaggtgag tatagaaaac
     7441 gctcaatttg tatgccaatg gtccaactcc ctaggcaaat cctttcaaga acaatggatg
     7501 ggaccaagga ttccttttct actgaccctt caagccttgg aagaagtatt ttcaatcttt
     7561 gatgaacaag agtttgtggg ccttatccag aaaatcaggc tagaagacag caatcttcat
     7621 atcgggagat tttttatcaa cccctagaaa caggggcaag gcttaggtag ccagacttta
     7681 aggaaatttg ttagtttggc ctttgaaaat gaagatatag ataatatttc tctaaatgtc
     7741 ttcgaggcaa atcaaagagc tcagaatctt taccaaaaag aaggatttga aatcgttcaa
     7801 atggttgaag cacctgtacg aaaatatagt ggattgaaac tagaatagta cacctctact
     7861 tctaaaacat tgttagaaat cg
//
LOCUS       DAVKZB010000084         7856 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKZB010000084 DAVKZB010000000
VERSION     DAVKZB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.084"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            133..1362
                     /gene="arcA"
                     /locus_tag="VXA80_001559"
     CDS             133..1362
                     /gene="arcA"
                     /locus_tag="VXA80_001559"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV0354032.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1420..2436
                     /gene="argF"
                     /locus_tag="VXA80_001560"
     CDS             1420..2436
                     /gene="argF"
                     /locus_tag="VXA80_001560"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV0354033.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2601..3548
                     /gene="arcC"
                     /locus_tag="VXA80_001561"
     CDS             2601..3548
                     /gene="arcC"
                     /locus_tag="VXA80_001561"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV0354034.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3759..5270
                     /locus_tag="VXA80_001562"
     CDS             3759..5270
                     /locus_tag="VXA80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV0354035.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5292..6623
                     /locus_tag="VXA80_001563"
     CDS             5292..6623
                     /locus_tag="VXA80_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV0354036.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLEFEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLVTQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7103..7318)
                     /locus_tag="VXA80_001564"
     CDS             complement(7103..7318)
                     /locus_tag="VXA80_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354037.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGK"
     gene            complement(7306..7557)
                     /locus_tag="VXA80_001565"
     CDS             complement(7306..7557)
                     /locus_tag="VXA80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0354038.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
BASE COUNT         2341 a         1563 c         1582 g         2370 t
ORIGIN      
        1 tgctaaatat cataatttat attattttcc gagaaattat agccattgac attacctagg
       61 aattgcgtta taacatacat gtaagcggta cccaaaatgg tgccaagtca aaatttttaa
      121 ggaggaaaat acatgtcttc acatccaatt caggtcttct cagaaattgg gaaactgaaa
      181 aaagttatgt tacaccgtcc aggcaaggag ttagaaaact tgttgccgga ctatcttgaa
      241 aggcttcttt ttgatgatat tcctttcttg gaagatgctc aaaaagaaca tgatgcattt
      301 gcccaagctc ttcgcgatga aggaattgag gttctctacc tagaacaact cgctgctgaa
      361 tcattgacct ctccagaaat ccgcgatcaa tttatcgagg aatacttaga cgaagccaac
      421 atccgtgatc gtcaaaccaa ggttgctatt cgtgaattgc ttcacggcat caaggacaac
      481 caagaattgg ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc agaaattcct
      541 gacgaagcta aagatctaac tgacttagtt gaatcagatt atccatttgc aattgacccg
      601 atgccaaacc tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc cgtatcgctt
      661 aaccacatgt ttgcagacac tcgtaaccgt gaaacactct acggtaagta tatcttcaaa
      721 taccacccaa tctatggcgg aaaagtggat ttggtctaca accgtgaaga agatacgcgt
      781 atcgaaggtg gagacgagct agttctttct aaagacgtcc ttgcagtagg tatctctcaa
      841 cgtacagacg cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa aaatgttggc
      901 ttcaagaaag ttttggcctt tgaatttgct aacaaccgta aattcatgca cttggatact
      961 gtcttcacta tggtagacta tgacaagttc actattcacc cagaaatcga aggcgacctt
     1021 cacgtttact cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga gaaaggtgac
     1081 ttagctgaac ttcttgctca aaaccttggt gtagaaaaag ttcatttgat tcgttgcggt
     1141 ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa cactttgacc
     1201 atcgcacctg gtgtggtagt tgtttatgac cgcaataccg tgaccaataa gattttggaa
     1261 gaatacgggc ttcgcttgat taagattcgc ggaagtgaat tggttcgggg ccgtggtgga
     1321 cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt cggtattcgt
     1381 caatagaaaa tgtaaaaaat agaaagagga aataataaaa tgacaaattc agtattccaa
     1441 ggacgcagct tcttagcaga aaaagacttt acccgtgcag agttagaata ccttattggt
     1501 ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc aacaccacta ccttgctggc
     1561 aagaatatcg ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc ctttacaact
     1621 gcggctatcg accttggtgc tcacccagaa tacctcggag caaatgatat tcagttgggt
     1681 aaaaaagaat ctactgaaga tactgctaaa gtattgggac gtatgtttga cgggattgaa
     1741 ttccgcggat tcagccaacg tatggttgaa gaattggcag aattctcagg cgttccagta
     1801 tggaacggtc taactgacga atggcaccca actcaaatgc tcgctgacta cttgactgtt
     1861 caagaaaact tcggtcgctt ggaaggcttg acattggtat actgtggtga tggacgtaac
     1921 aacgttgcca acagcttgct cgtaacaggt gctatccttg gtgtcaatgt tcacatcttc
     1981 tcaccaaaag aactcttccc agaaaaagaa atcgttgaat tggcagaagg atttgctaaa
     2041 gaaagtggcg cacatgttct catcactgaa gatgctgatg aagcagttaa agatgcagac
     2101 gttctttaca cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc agaacgtgta
     2161 gctcttctta aaccttacca agtcaatatg gacttagtta aaaaagcagg caatgaaaac
     2221 ttgatcttcc tacactgctt gccagcattc cacgatactc acactgttta tggtaaagac
     2281 gttgctgaaa aatttggtgt agaagaaatg gaagtaacag acgaagtctt ccgcagcaag
     2341 tacgctcgcc acttcgatca agcagaaaac cgtatgcaca ctatcaaagc tgttatggct
     2401 gctacacttg gtaaccttta tattcctaaa gtataatttt agataataaa ccgtctacca
     2461 acagctatga gggctgcgac taatagcttt agtccggtcc tcttttatat aatggtaatc
     2521 tattatttct tataaaatat gtgaaaaatc attaaattga aatctaaacg cattctattg
     2581 agtgtgataa aggagaattt atggcaaatc gtaaaattgt agtagctttg ggaggaaatg
     2641 cgattctttc ttctgaccca tcagcaaagg ctcaacaaga agctttagtt gaaacagcta
     2701 agcatcttgt aaaattgatt aaaaatggag atgatctgat tatcactcac ggtaatggac
     2761 ctcaagttgg gaatctcttg ctccaacatt tggcatcaga ctctgaaaag aaccctgcct
     2821 tcccactcga ctcacttgtc gctatgacag aaggtagcat cggtttctgg ttgaaaaatg
     2881 ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct gttgtaacgc
     2941 aagttgtcgt agataaaaat gatccagctt ttgttaattt gagtaaacca atcggtcctt
     3001 tctattcaga agaagaagca aaagcagaag ccgaaaagag cggagcgact ttcaaggaag
     3061 atgctggccg tggctggcgt aaggtcgttg cctcaccaaa acctgttgac atcaaagaaa
     3121 ttgaaaccat ccgtactctt ttaaatagtg gtcaagtcgt cgtagctgca ggtggtggcg
     3181 gtattcccgt cgtcaaagaa aacaatggac atttgactgg tgtcgaagcg gttattgata
     3241 aagacttcgc ttcccaacgt ttggcagaat tggttgaggc agacctcttc atcgttttga
     3301 caggtgtaga ttatgtattt gttaactaca acaagccaaa ccaggaaaaa ttggaacatg
     3361 tgaatgttgc ccagctggaa gaatatatca aacaagatca gtttgcacca ggtagcatgc
     3421 ttccaaaagt agaagcagct atcgcttttg tcaatggtcg tccagaagga aaagcagtta
     3481 ttacttccct tgaaaatcta ggcgccttga ttgaatctga aagcggaaca attattgaaa
     3541 aaggataagt tgttttacta ataagatgta ttctatttct agtatcttta tatcaaatta
     3601 gaaattattc ttgaaaacat gtacaatatt tcaaaagata ttagtttttt agactttaat
     3661 atggtaaaac aaatataaat agaaagcgtt ttcttgaatg tttatttaag aaagtagttg
     3721 gttttttaca ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa gctaaaaaag
     3781 ggtttaagat gccttcatct tacaccgtat tattgataat cattgctatt atggcagtgc
     3841 taacttggtt tatccctgcg ggggccttta tagaaggtat ttacgaggct cagcctcaaa
     3901 atccacaagg gatttgggat gtcctcatgg caccgattcg ggctatgcta ggtactcatc
     3961 cagaggaagg ttcgctcatt aaagaaacga gcgcagcgat tgatgtagcc ttcttcatcc
     4021 ttatggttgg tggtttcctt ggcattgtca acaaaactgg tgctcttgac gtagggattg
     4081 cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt aattttggta ctgatgcctt
     4141 tgtttgccct cggtggtaca acttatggta tgggggaaga aacaatggcc ttctatccac
     4201 tccttgtgcc agttatgatg gccgttggtt ttgatagcct gactggtgtt gcaattattt
     4261 tgctcggttc tcaaatcggc tgtttggcat ctactctgaa tccatttgcg acaggtattg
     4321 cttcagcgac tgcgggagtt ggtacagggg acggtatcgt acttcgtctg atcttctggg
     4381 ttaccttgac tgctcttagt acttggtttg tttaccgtta tgcggataag attcaaaaag
     4441 atccgactaa gtcactggtt tatagtactc gcaaagaaga tttgaaacac tttaacgtag
     4501 aagaatcttc atctgtagaa tctacactta gcagcaaaca aaaatcagtt ctcttcttat
     4561 ttgtgttgac attcatcttg atggtattga gcttcattcc atggacagac cttggcgtta
     4621 ccatttttga tgactttaat gcttggttga ctggtcttcc agttattggt aatattgtcg
     4681 gttcatctac ttctgcacta ggtacttggt acttcccaga aggcgcaatg ctctttgcct
     4741 ttatgggtat cctgattggt gttatttatg gtcttaaaga agataagatt atctcttcct
     4801 tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt gctcgtggta
     4861 ttcaagttat catgaacgac ggtatgatta ccgatacaat cctcaactgg ggtaaagaag
     4921 gcttgagcgg tctatcttca caagtcttta tcgttgtaac ttatatcttc tatctaccta
     4981 tgtcattctt gatcccatct tcatctggtc ttgccagcgc aactatgggt atcatggctc
     5041 cacttggaga atttgtaaat gtccgtccta gcttgattat cactgcttac caatctgctt
     5101 caggtgtctt gaacttgatt gcaccaacat ctggtattgt gatgggagct cttgcacttg
     5161 gacgtatcaa cattggtact tggtggaaat tcatgggcaa actcgtagtc gctattattg
     5221 tagtgaccat cgcccttctt ctccttggaa ccttccttcc attcctataa aatagtgagt
     5281 gaggtgattc catgaaaata gatataacaa atcaagttaa agatgaattt cttatatcat
     5341 taaaaacctt gatttcctat ccttcagtac tcaatgaagg agaaaatgga acaccttttg
     5401 gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat ttgtcgagac ataggtttca
     5461 ctacctatct tgaccctaaa ggttattacg gatatgcaga aatcggtcag ggagcagagc
     5521 ttctggccat tctctgtcat ttggatgttg ttccatcagg tgatgaagca gattggcaga
     5581 caccgccatt tgaagcaact atcaaagacg gctgggtatt cggacgtggt gtccaagatg
     5641 ataaaggccc ttcgctcgca gctctctatg cagtaaaaag cttgctggac caaggtattc
     5701 agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga ggaaaccctc tggcgctgca
     5761 tggcacgcta caataccatc gaagaacagg ccagtatggg ctttgcacct gactcatctt
     5821 ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa acttcatggc cctggatcgg
     5881 atcaactaga gtttgaagta ggaggcgcct ttaacgttgt accagacaag gccaactacc
     5941 aaggtctcct ctatgaacag gtttgtaacg gtctcaaaga agctggttat gattaccaaa
     6001 ccactgaaca aaccgtaacg gttctcggag tgccaaagca tgctaaggat gctagtcaag
     6061 gtatcaatgc tgtcatccga ctagctacca ttcttgctcc tctccaagaa caccctgctc
     6121 tcagttttct tgtaacacaa gcaggtcaag acggcacagg aagacaaatc tttggtgata
     6181 tagcagatga accttctggt cacctatcct ttaatgtcgc aggtctcatg atcaatcatg
     6241 aacgttctga aatccgtatt gacattcgga ctcctgtctt agctgacaag gaagaactag
     6301 tagagttgct tacaagatgt gcacaaaact accaactccg ctacgaagag tttgactatc
     6361 tagcgcctct atacgtcgca aaagacagta aactcgttag cacactgatg caaatctacc
     6421 aagaaaagac tggcgataac agtcctgcta tttcatccgg tggtgccact tttgctcgca
     6481 ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag acagaacatc
     6541 aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt tatgccgaag
     6601 ccgtctatcg acttgcaact taatcaggca actgtttcta caaaaaaaaa tcgaccgatt
     6661 aatgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtg ttttattatg
     6721 aaattgaatt atgaagataa agttcagatc tatgaactaa gaaagtaaga acaaagcttc
     6781 aaacagcttt caaaaagatt tatgtggatg tttctggttt aaagtcatct gaatctttga
     6841 gatgagcttt ataaatcgct tttttcagtt tttgcactgg tatttcgata aactcaaact
     6901 ttttagccgt tgtattgcct gattttatag tatgttgaaa ctagaatagt acacctctac
     6961 ttctaaaaca tttttagaaa tcgatttgac tgtcctgatt gatttgtcct gttcttattt
     7021 cattttatta tatttgagcc acttcatctt taacggcttt attcataagc tcttgtaatt
     7081 tttctttact atcaattact tcttattttc cgttgtaatt tattgtaata ggttttaact
     7141 tacctaattt ctcgacacgc tcattaattt gatctttttt gaaggctgca tatgtttttc
     7201 ctaagatttt ttcgaaaata tatttatcag atagcggttt gccttcttct tcagcttggt
     7261 ttttgtattg atttgaaaca taaggaacaa atccttcata gtaacctaat gctcccataa
     7321 gttcaaaagc ttgttttcta attcaaacca ttgcaactca gatttcagct tttcagataa
     7381 atcctgctca tccaaataat gacttgaaat tagtgctgaa ctcgtttctg tagcctgtac
     7441 aggctgagca cccataccag caaaaaataa actcgttcct agcaagaccg aacaagctcc
     7501 tattgcatat ttcctcaaag aaaaacgctg ctttctctca aattgaaatt ctttcatccc
     7561 atctcccatc attcattatt actgtatatt ttgtatatca gaaatagttt gtattcacaa
     7621 atctttctag ttattccctt atcattccta attaagggag ataacataca ataattttta
     7681 gttaaatgta tatcgatgtt ttttgttttt cttaataaac gcaatacaaa aagagcctgt
     7741 taccaagctc tttgtactca atgaaaatca aagagcaaac taggaaacta gccacaggtt
     7801 gctcaaaaca ccgttttgag gtagtagata agactgacga agtcagctca aaacac
//
LOCUS       DAVKZB010000085         7808 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKZB010000085 DAVKZB010000000
VERSION     DAVKZB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.085"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            97..1007
                     /locus_tag="VXA80_001566"
                     /pseudo
     CDS             97..1007
                     /locus_tag="VXA80_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019783158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            1059..1357
                     /locus_tag="VXA80_001567"
                     /pseudo
     CDS             1059..1357
                     /locus_tag="VXA80_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737957.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            1424..2680
                     /locus_tag="VXA80_001568"
     CDS             1424..2680
                     /locus_tag="VXA80_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0354039.1"
                     /translation="MTYTHLTTELIGIKDKNIKILFVLKHQTHLEIRAKLDYDSPRCP
                     HCQGKCIKYDFQKLSKIPILDCQGFPTCLLLKKRRFQCKSCKKVTVAETALVKKNCQI
                     SQPIWEKVTQLHTENMTNIAIARRLHISVSVVQRKLAQFQFEHDFTKLPKVLSWDEFS
                     RNKGKLAFIAQDFETRRIVTILENNRQTTIKNYFYKYPRAVRETVEVVTVDMSGSYIP
                     IIKQLFPRAKIVLDRFHIIQHLSRAMMTTRINIMKTFDKRSLPYRSMKNHRRILQKDS
                     RKLSLNRFFSRTFGQTVTPREVVQKTLNFSDELKFYYELYQILLFHFQEMNSKYFFEL
                     LEDKLDLVNLAFKTVFKTFLKYKTYITNAMELPYSNAKLEATNKLIKDIKRQAFGFRN
                     FTNFKTKIYISLNIKNERTNFVLSRC"
     gene            2739..3440
                     /locus_tag="VXA80_001569"
                     /pseudo
     CDS             2739..3440
                     /locus_tag="VXA80_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            3452..4318
                     /locus_tag="VXA80_001570"
                     /pseudo
     CDS             3452..4318
                     /locus_tag="VXA80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(4398..5045)
                     /locus_tag="VXA80_001571"
     CDS             complement(4398..5045)
                     /locus_tag="VXA80_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354040.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(5042..5740)
                     /locus_tag="VXA80_001572"
     CDS             complement(5042..5740)
                     /locus_tag="VXA80_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354041.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAIVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(5750..6502)
                     /locus_tag="VXA80_001573"
     CDS             complement(5750..6502)
                     /locus_tag="VXA80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354042.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLKCRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(6502..7266)
                     /locus_tag="VXA80_001574"
     CDS             complement(6502..7266)
                     /locus_tag="VXA80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354043.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(7362..7765)
                     /locus_tag="VXA80_001575"
                     /pseudo
     CDS             complement(7362..7765)
                     /locus_tag="VXA80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2728 a         1586 c         1281 g         2213 t
ORIGIN      
        1 taaattatta actacccgtt gtgctagtta attctagcca aataaaaaaa ctttgcgtat
       61 cataaactca aaacaaccac gaaatgaggg aatgatgtgc aaagccaaca ttattatctc
      121 gcaaatccca atcaaacaca agttattttt tcaaaaatct tgactcaagt tatcgcttta
      181 tatgagcgat ttgttcccca tgaggttcga aatcggcgaa atatccacct acaaaaacag
      241 tctgatgtcg ttgttatcgc tagttatctt tgggcaattc aagagggctg caggacgcaa
      301 gtgctattta ccgtgcgatt cgtcataatc tcttccctga caacttccct gaacgctcac
      361 gattttgccg tatctgtcaa aatcttgctc aaagtattca gcgtatgcgc tattttatgg
      421 tcttagatct ttgtcaaacc tgctcgttcg gcttgattga cagctttcct tgtgccctat
      481 gtcaccccat tcgcaacatg agagctacat tgttatcaga ggtggcagat attggctata
      541 atgcgactaa gaaaatccat tattatggct tgaaattttc agttcttgtc agcgacagtg
      601 ggtttcctat tgattatgtt gtcacacccg catctattta tgatggcgat gttgctttgg
      661 agctacttga aaatagccca tttccaatcg tttatggaga taaaggatat gtggatagac
      721 agacaaaggc agcgttagca gactgtggta tccagttaat ttctcaactc agaaaaaaca
      781 tggtgggtta ttcatggcta gaaaattaca aaataagtcg attgaggaaa ccagtggaga
      841 ctgtattttc tagtttagag cagtttggaa tggaggcttt acgttgtcga aacattcagg
      901 cgcttaaatt taagacagag gcaatattac ttatctattc tcttttgctt aaaagcagtc
      961 atactgaatt tggtatcagt ttaaaatatt cccttgctta cgcttgatta actagcacaa
     1021 cgggttatta actataaaac cccatcttca caacataaaa tggttatgct aaagatgatt
     1081 acaggataga ctatctcaga gaccacatca aggaaatgcg agatgcagac acccttgacg
     1141 gaatagacct actaggctac acgacctgag gagcaattat ttagtttctg caggaacagg
     1201 tgagatgact aaaaaatatt gctttatcta tgtagaccgt gatgattatg gcaatggcac
     1261 acaaaaaaga agcaagaaaa aatcatttga ctggtacaaa aaagtgattg cttctaatgg
     1321 tgaagattta gattcccaat ttgtaaaatt aagctagaca gaaaaagcca tctatgttag
     1381 gctcaaataa actaccacat ttgtctgaaa ggaactaaca taaatgacct atactcatct
     1441 taccacagaa ctaattggaa tcaaagacaa aaatatcaaa attctcttcg ttttgaaaca
     1501 tcagacccat ctagaaatca gggcaaagct agattacgac agccctcgtt gtcctcattg
     1561 ccaaggaaag tgtatcaagt acgattttca aaagttgtca aaaatcccta ttttagattg
     1621 tcaggggttt ccaacctgcc tcctgcttaa aaaacgccgg tttcaatgca aatcttgcaa
     1681 aaaagtgacc gtagctgaga cagctctagt caagaaaaac tgtcagattt cccaacctat
     1741 ttgggagaaa gtcacacaac tccatacaga aaatatgacc aatatagcca tcgctaggag
     1801 actacatatc tccgtgtcgg tcgtccagag gaaactggct cagtttcaat ttgagcatga
     1861 ttttacgaaa ttaccaaaag tcttgagctg ggatgaattt agtcggaaca agggaaaact
     1921 cgcctttatt gctcaggatt ttgagacaag acggattgtg accattcttg aaaataaccg
     1981 ccaaaccacc atcaagaact atttctacaa gtatcctagg gcggtcagag aaactgtcga
     2041 ggtcgtaaca gtggacatgt cgggtagcta tattcctatc atcaaacaac tatttccaag
     2101 ggctaaaatt gtccttgata gatttcacat tatccaacac ctgagccgtg ctatgatgac
     2161 aactagaatt aatattatga agactttcga taagcgttcc ctaccttatc gatccatgaa
     2221 aaatcaccgg cgtattctcc aaaaagatag tcgtaaactg tctctgaatc gcttcttttc
     2281 tcgcactttt gggcaaacag taacaccgag agaagttgtc cagaagacat tgaatttctc
     2341 agatgaactg aaattctatt acgaactcta ccagatcctt ctcttccatt tccaagagat
     2401 gaactcgaaa tatttcttcg agcttctaga agacaagctg gatcttgtca atcttgcctt
     2461 taaaactgtt tttaaaactt ttctaaagta taaaacgtac atcacgaacg ccatggagct
     2521 tccatattcc aatgctaaac tggaagcgac caataaactc atcaaagaca tcaagaggca
     2581 agccttcggc tttaggaact ttacgaactt taaaactaag atatatattt ctttaaacat
     2641 caaaaatgag agaacgaatt tcgtcctctc taggtgttag cttttcgtct acccactaca
     2701 gttgacaaag agccaaaaaa tccttcggag acaatcccat cctccaagga ctttctctag
     2761 aaatcaaaaa aggggaagtt gttgtcatcc tagggccatc tggttgtggg aaaagtaccc
     2821 tccttcgttg cctcaacggc ttagaaagta ttcaaggtgg agatattctt ctggatggtc
     2881 agtctatcgt tgaaaataaa aaagattttc acctagttcg ccaaaagatt ggcatggtct
     2941 tttaaagtta tgaactcttt ccccatctgg atgtcttaca aaacctcatc ctaggcccta
     3001 tcaaaactca aggatgggac aagaaagaag taacggaaga agctttgcaa ttactagatc
     3061 gtgtcggttt gctggataaa caacatagct ttgcccgtca attatctggt ggacagaagc
     3121 aacgtgttgc aattgtccgt gccctcctaa tgcatccaga aatcatcctt tttgacgagg
     3181 tgactgcttc gctggatcca gaaatggtgc gtgaggtgct ggaacttatc aatgatttgg
     3241 cccaagaagg ccgtaccatg attttagtaa cccacgaaat gcagtttgcc caagccattg
     3301 ctgaccggat tatcttcctc gaccaaggga aaatcgctga agaaggaaca gctcaagcct
     3361 tctttaccaa tccgcaaacc aaacgagccc aggaattttt aaacgtcttt gactttagcc
     3421 aatttggctc atatctataa aggagattct tatgaaacta ttcaaaccac tcttaactgt
     3481 tttagcactt gcctttgccc ttatctttat cactgcttgt agctcaggtg gaaacgctgg
     3541 ttcatcctct ggaaaaacaa ctgccaaagc tcgcactatc gatgaaatca aaaaaaagcg
     3601 gtgaactgcg aatcgccgtg tttggagata aaaaaccgtt tggctacgtt gacaatgatg
     3661 gttcttacca aggctacgct acgatattga actagggaac caactagctc aagaccttgg
     3721 tgtcaaggtt aaatacattt cagtcgatgc tgccaaccgt gcggaatact tgatttcaaa
     3781 caaggtagat attactcttg ctaactttac agtaactgac gaacgtaaga aacaagttga
     3841 ttttgccctt ccatatatga aagtttctct gggtgtcgta tcacctaaga ctggtctcat
     3901 tacagacgtc aaacaacttg aaggtaaaac cttaattgtc acaaaaggaa cgactgctga
     3961 gacttatttt gaaaagaatc atccagaaat caaactccaa aaatacgacc aatacagtga
     4021 ctcttaccaa gctcttcttg acggacgtgg agatgccttt tcaactgaca atacggaagt
     4081 tctagcttgg gcgcttgaaa ataaaggatt tgaagtagga attacttccc tcggtgatcc
     4141 cgataccatt gcggcagcag ttcaaaaagg caaccaagaa ttgctaggct tcatcaataa
     4201 agatattgaa aaattaggca aggaaaactt cttccacaag gcctatgaaa aaacacttca
     4261 cccaacctac ggtgacgctg ctaaagcaga tgacctggtt gttgaaggtg gccattaact
     4321 cttaaaaggg actggatttt aagctccaat ccctttttaa gattttacct ataacatcct
     4381 gagtctatct aagatgttca atctgaacac agtgtacata ctttatcttc tattgcatat
     4441 actttatcac ataagatacg aatatcctct tcactatgac tagcaatcaa aattgttgtc
     4501 cctttttcac tagagagctt tctaaacaat gttctcatat tttctacact tgatttatcc
     4561 aaggcattca taggttcatc tagtaaaaga atagagggat tctccataat tgcttgagca
     4621 atccctagct ttttcctcat acctagcgaa taagttttaa ctttctggtc tttttgctca
     4681 tatagaccaa ctattttcag tgtatcattg atttcctgat taccaactac tcctcgtatg
     4741 cttgccaaat attgtaaatt cttaaagcca ctataataat ttataaaacc aggttcttca
     4801 atcaaagctc ccaaattagc tggaattttt ctctcaggaa caatattttc cccattgatt
     4861 aacacttctc cataagacgg actatataaa ccagctatta atttaaacaa tacacttttc
     4921 cctgagccat tcgcaccagt aattcctata atttccccct gtttacaact aaagttaagg
     4981 ttttgaaaaa cacatgtctt ttttaatttc aactcaatat tttttaatgt aattatttca
     5041 ttcattctat aaacctcctc ttttgacgag tgaaatagaa aatgctttga aaaagaaaga
     5101 ctaaaaatag caactgaaga aataaatctc gtcctatatc tccattccct cgattcaaaa
     5161 tataaaatag ataattagtt cgatttccta caaatagacc accaaacaca atcatgagta
     5221 aaaagaaact aacgcaagca aagttcattt gtacattcgc tcctagtgcg tataacataa
     5281 agcaaaacaa gactaacaca caagcattca aagaatcaac tataaacaaa actcctatat
     5341 cccagtttgg taactgtaac ataaattgaa tcactagaac ttttaccatg attaaaatga
     5401 gtccataaag aaaacaggtc caaatctgat acctaatcca gagcttaata gaagaaaatc
     5461 gaatcacttc cataaagaaa tcggatgctc ttctacgata tacaattgct agaaaaaatg
     5521 tatgaataat aaaccaatga agcaaccata tagataagac taaaaaagag ttttgactca
     5581 gattaggccc ttttaaaatt tctataacaa gtctatcatt tccttctaac caataccata
     5641 atcctaaaca aatcaatatc caaaaaagaa cataacccca aaagtacttg gtatagatta
     5701 tctgattcaa cctccacatg tgactactta actttcccat ttccaacttt tacactctcc
     5761 tccagtttct atgaacagaa aatacagtca atggaattat gatagatagt aatgtaagcc
     5821 aataaatcaa tgcatacgga ttggaattta ccatatactg aaaaattggc aataatccaa
     5881 ttatcccatt gtccaactga taaatccaga ataaggattc tacaaataaa aaagtaaaca
     5941 gaaatactaa gctcatgttt gattttttaa accaagaaaa actgctaata gtaagtaagg
     6001 ataaaaagaa atagtatgct atataagaga aaaaaaatcc tataaagaaa ctagcattgt
     6061 ttgcaatact tataccataa aattctctta aaaaatcaac ctcctttatc tccaaagaga
     6121 agctaaaacc attactaaat gcaattagaa aaatcaataa aaataaagtc gccgtccaaa
     6181 tccccgtact aagagctgct aatttgaaac taaaactggt aaagtgctta attgatttca
     6241 gacgaatgcg acacttcaac ctattaaaat agttattcat caaataaaaa aagaacaata
     6301 tatatgtgaa cggaaagcaa tatactccag tcgtcatatc ttgaagtaaa actaagatcc
     6361 attctaatac atttggatgg attgaatatt ggcgacagcg caataaatat actgtactag
     6421 ataaaacaca ggatagcagt aatataaaat aaaccaatac tgataaaaaa tcttttttgt
     6481 aaaattgaac aaattgtttc attatacgta gtcctctgaa tgtagaaaaa atgtaccata
     6541 aacaaccaaa caactaacaa ataaaataaa agcaagatgc ccactaacta aggaaagact
     6601 gatatctttc tgatatccca aagctaatgt tgtcacaggt tctaagtaag atagccctaa
     6661 aatagcccaa aaaataccac caaccatcat ataggcaact gggatgaaaa tagctcctat
     6721 ttttttcttc actagcaaag cactagctag tccaaaaata gagaacacag cgccccaaac
     6781 tccataccag agagtcgtta caagactata gagaaactga ttggaatcaa ataattcttt
     6841 taaggcacca ctataatctc caatataaat ttcctgataa ggagtcacta aaagattaat
     6901 tcctaataat aataaatagg ggagaaaaaa gactagaaaa gaagaaataa ttacagtact
     6961 acccacaata gccagatact tctttttaga aattcgacac aattgtgctg ttagaaaatg
     7021 actctcagca tcctctatta tctgactaga atagggcagt gtacagataa gtgcagctac
     7081 taggctaatc ggtgaaaatc ctcgaataga agaagcggca aaaaaaatcg ataaaccttc
     7141 aattttataa ataccattga aagcaaggaa atttcctaaa ctcatgcaaa gaagggtcaa
     7201 aaataaagac atataaaatc gaggtgattg aacgactcgt acaagattac aaatgaaaaa
     7261 ttccatcctt actcctcctt ataataaaaa tagggtgtag cattcttttt tcatgctaca
     7321 cccacaatca accatcttta aggcttactc tgacaagtaa gttaataaga atctggattc
     7381 caagaacctg aagtatgaat tcttacatga tttccaaatt gtggcgccat agctaatcta
     7441 gtaccagaac caatataatt gtcaccacct ccattatagt acatgacaat cctagagcca
     7501 gaccccaatg aatataccgg ggtaatatct gacccattat aggcgctacg aatagaggta
     7561 tttaaccttt taccgccacc agtgctgtca ctattattaa ttccagcaga ggcgttttct
     7621 ttctcaagct cactagttgt atccgaaccc caacgcggca acgttaaatc ataatttcca
     7681 atctgtctag ctaaaactgt agggagaaac aaactagatg caattgtaac tacgcaaaaa
     7741 aggaatatat ttttcttctt ttcatgacat cgtcctctta agaagtgata aacaagatag
     7801 ttgattga
//
LOCUS       DAVKZB010000086         7789 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKZB010000086 DAVKZB010000000
VERSION     DAVKZB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.086"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(135..1103)
                     /locus_tag="VXA80_001576"
     CDS             complement(135..1103)
                     /locus_tag="VXA80_001576"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0354044.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VXA80_001577"
     CDS             complement(1248..2063)
                     /locus_tag="VXA80_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV0354045.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLLGWLSSFANTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKFLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VXA80_001578"
     CDS             complement(2088..2585)
                     /locus_tag="VXA80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV0354046.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VXA80_001579"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VXA80_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV0354047.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VXA80_001580"
     CDS             complement(4033..4548)
                     /locus_tag="VXA80_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0354048.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGEMLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VXA80_001581"
     CDS             complement(4550..4993)
                     /locus_tag="VXA80_001581"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV0354049.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VXA80_001582"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VXA80_001582"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VXA80_001583"
     CDS             complement(5180..5647)
                     /locus_tag="VXA80_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV0354050.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VXA80_001584"
     CDS             complement(5848..7134)
                     /locus_tag="VXA80_001584"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV0354051.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VXA80_001585"
     CDS             complement(7365..7601)
                     /locus_tag="VXA80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV0354052.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         2492 a         1905 c         1243 g         2149 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc tacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtataact tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc ccaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 cctttatagg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa aaattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gcaaaactag acaaccagcc taaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgccatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatcgcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagccataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtctta gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca tctccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaagaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccac cagctgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgttccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgacccc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgattg agacgctcat
     5281 ctgtcaagat attgtacaaa ctcccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ctccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ccgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgaactc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gttcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaga ttttcacagt
     6121 atccaaaccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgacac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttggtcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga taactcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaatcac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagcca tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actatcatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagccatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacaatt aaagttctaa aaagaccgcc ttaaaactt
//
LOCUS       DAVKZB010000087         7430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKZB010000087 DAVKZB010000000
VERSION     DAVKZB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.087"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            112..6528
                     /locus_tag="VXA80_001586"
     CDS             112..6528
                     /locus_tag="VXA80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV0354053.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQS
                     QGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHFI
                     RGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISYY
                     LVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKSLFN
                     IHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDDGTD
                     LEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKATKY
                     DFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKAEYP
                     YVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVT
                     TYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVLMS
                     ALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVEAI
                     KEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNE
                     SVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQ
                     TGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDG
                     EIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSEL
                     KPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVI
                     NLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
     gene            7153..>7430
                     /locus_tag="VXA80_001587"
     CDS             7153..>7430
                     /locus_tag="VXA80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0354054.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVT"
BASE COUNT         2901 a         1046 c         1436 g         2047 t
ORIGIN      
        1 atgtactact ctatttccta gataatactt agtaaaactt tttataacaa aggctagcaa
       61 agttaaagtt tattatctat tggaagttat ataaatatgt aaggaattaa aatgaaaaaa
      121 agtacagtat tgtcattaac cacagctgca gttattttag cagcctatgc ccctaatgag
      181 gtagtcttag cagacacatc tagctctgaa gatgctttaa gcatctctga taaagaaaaa
      241 gtagcagaaa ataaagagaa acatgaagat atccatagtg ctatggaaac ttcaaaggat
      301 actgaagaaa agaaaacaac agttattgag gaaaaagaag ttgttagtaa aaatcctgtg
      361 atagacacta aaactagcaa tgaagaagca agaatcaaag aagattccaa tcaatcccaa
      421 ggagataatg cacactcgtc tgcaaataaa ggcacagaaa atcccaaaaa agaagataga
      481 cttgtctata ttgctgaatt taaagataaa gaatctggag aaaaagcaat caaggaacta
      541 tccagtctta agaatacaaa agttttatat acttatgata gaatttttaa cggtagtgcc
      601 atagaaacaa ctccagataa cttggacaaa attaaacaaa tagaaggtat ttcatcgatt
      661 gaaagggcac aaaaagtcca acccatgatg aatcatgcca gaaaggaaat tggagttgag
      721 gaagctattg attacctaaa gtctatcaat gctccgtttg ggaaaaattt tgatggtaga
      781 ggtatggtca tttcaaatat cgatactgga acagattata gacataaggc tatgagaatc
      841 gatgatgatg ccaaagcctc aatgagattt aaaaaagaag acttaaaagg cactgataaa
      901 aattattggt tgagtgataa aatccctcat gcgttcaatt attataatgg tggcaaaatc
      961 actgtagaaa aatatgatga tggaagggat tattttgacc cacatgggat gcatattgca
     1021 gggattcttg ctggaaatga tactgaacaa gacatcaaaa actttaacgg catagatgga
     1081 attgcaccta atgcacaaat tttctcttac aaaatgtatt ctgacgcagg atctgggttt
     1141 gcgggtgatg aaacaatgtt tcatgctatt gaagattcta tcaaacacaa cgttgatgtt
     1201 gtttcggtat catctggttt tacaggaaca ggtcttgtag gtgagaaata ttggcaagct
     1261 attcgggcat taagaaaagc aggcattcca atggttgtcg ctacgggtaa ctatgcgact
     1321 tctgcttcaa gttcttcatg ggatttagta gcaaataatc atctgaaaat gaccgacact
     1381 ggaaatgtaa cacgaactgc agcacatgaa gatgcgatag cggtcgcttc tgctaaaaat
     1441 caaacagttg agtttgataa agttaacata ggtggagaaa gttttaaata cagaaatata
     1501 ggggcctttt tcgataagaa taaaatcaca acaaatgaag atggaacaaa agctcctagt
     1561 aaattaaaat ttgtatatat aggcaaggga caagaccaag atttgatagg tttggatctt
     1621 aggggcaaaa ttgcagtaat ggatagaatt tatacaaagg atttaaaaaa tgcttttaaa
     1681 aaagctatgg ataagggtgc acgcgccatt atggttgtaa atactgtaaa ttactacaat
     1741 agagataatt ggacagagct tccagctatg ggatatgaag cggatgaagg tactaaaagt
     1801 caagtgtttt caatttcagg agatgatggt gtaaagctat ggaacatgat taatcctaat
     1861 aaaaaaactg aagtcaaaag aaataataaa gaagatttta aagataaatt ggagcaatac
     1921 tatccaattg atatggaaag ttttaattcc aacaaaccga atgtaggtga cgaaaaagag
     1981 attgacttta agtttgcacc tgacacagac aaagaactct ataaagaaga tatcatcgtt
     2041 ccagcaggat ctacatcttg ggggccaaga atagatttac ttttaaaacc cgatgtttca
     2101 gcacctggta aaaatattaa atccacgctt aatgttatta atggcaaatc aacttatggc
     2161 tatatgtcag gaactagtat ggcgactcca atcgtggcag cttctactgt tttgattaga
     2221 ccgaaattaa aggaaatgct tgaaagacct gtattgaaaa atcttaaggg agatgacaaa
     2281 atagacctta caagtcttac aaaaattgcc ctacaaaata ctgcgcgacc tatgatggat
     2341 gcaacttctt ggaaagaaaa aagtcaatac tttgcatcac ctagacaaca gggagcaggc
     2401 ctaattaatg tggccaatgc tttgagaaat gaagttgtag caactttcaa aaacactgat
     2461 tctaaaggtt tggtaaactc atatggttcc atttctctta aagaaataaa aggtgataaa
     2521 aaatacttta caatcaagct tcacaataca tcaaacagac ctttgacttt taaagtttca
     2581 gcatcagcga taactacaga ttctttaact gacagattaa aacttgatga aacatataaa
     2641 gatgaaaaat ctccagatgg taagcaaatt gttccagaaa ttcacccaga aaaagtcaaa
     2701 ggagcaaata tcacatttga gcatgatact ttcactatag gcgcaaattc tagctttgat
     2761 ttgaatgcgg ttataaatgt tggagaggcc aaaaacaaaa ataaatttgt agaatcattt
     2821 attcattttg agtcagtgga agaaatggaa gctctaaact ccaacgggaa gaaaataaac
     2881 ttccaacctt ctttgtcgat gcctctaatg ggatttgctg ggaattggaa ccacgaacca
     2941 atccttgata aatgggcttg ggaagaaggg tcaagatcaa aaacactggg aggttatgat
     3001 gatgatggta aaccgaaaat tccaggaacc ttaaataagg gaattggtgg agaacatggt
     3061 atagataaat ttaatccagc aggagttata caaaatagaa aagataaaaa tagaacatct
     3121 ctagatcaag atccagactt atttgccttt aacaaccaag gtgttcatgc agaatccact
     3181 agtggttcta agattgctaa catttatcct ttagattcaa atggaaatcc tcaagatgct
     3241 caactagaaa gaggactaac tccatctcca cttgttctaa gaagtgcaga agaaggacta
     3301 atttcaattg taaacaccaa caaagaagga gaaaaccaaa aagaccttaa agttgtatca
     3361 agagagcact tcataagagg aattctaaat tctaagggaa atgacgctaa gggtatcaaa
     3421 tcatccaaac taaaagtttg gggtgacttg aaatgggatg gactcatcta taacccaaga
     3481 ggtagagaag aaaatgcccc agaaagtaag gataaccaag atccagctac taagatacga
     3541 ggacaatttg aaccgattgc ggaaggtcaa tatttctata aatttaaata tagattaact
     3601 aaagattacc catggcaggt ttcctatatt cctgtaaaaa ttgataacac agcccctaag
     3661 attgtttcgg ttgatttttc aaatcctgaa aaaattaagc taatcacaaa ggatacttat
     3721 cacaaggtaa aagatcaata caagaatgaa actttatttg ctagagatca aaaagaacat
     3781 cctgaaaaat ttgatgagat tgcaaatgag gtgtggtatg caggagctgc tcttgttaat
     3841 gaagatggag aggttgagaa aaatcttgaa gtaacttatg caggtgaggg tcaaggaaga
     3901 aataggaagc ttgataaaga tgggaatact atctatgaaa ttagcggagc aggagatcta
     3961 agaggaaaaa tcatcgaagt aatcgcccta gatggagcaa gcaactttac caaaatccat
     4021 aggattaaat ttgctaatca agctgatgaa aaagggatga tttcctatta tctagtagat
     4081 cctgatcagg attcatctaa ataccaaaag cttggcgaaa ttcctgaatc taaatttaag
     4141 aatttaaaaa atgttaaaga tgatagccta aacaaagaga cagctgaggt ggaaaataac
     4201 cttttagtag ataatcagtc tatcgaagga aaatctctct ttaatattca taaaactatt
     4261 tcaacaatta gagactttga aaataaagac ctaaagaaac tcattaaaaa gaaatataag
     4321 caagaagacg actttgtaaa tggtggaaca agaacagtag agcgtgatta taaatacgat
     4381 gataaaggaa atataatagc ctacgatgat gggactgatc tagaatatga aactgagaaa
     4441 cttgacgaaa tcaaatcaaa aatttatggt gttctaagtc cgtctaaaga tggacacttt
     4501 gaaattcttg gaaagataag taatgtttct aaaaatgcca aggtatatta tgggaataac
     4561 tataaatcta tagaaatcaa agcgaccaag tatgatttcc actcaaaaac gatgacattt
     4621 gatctatacg ctaatattaa tgatattgtg gatggattag cttttgcagg agatatgaga
     4681 ttatttgtta aagataatga tcagaaaaaa gctgaaatta aaattagaat gcctgaaaaa
     4741 attaaggaaa ctaaagcaga atatccctat gtatcaagtt atgggaatgt catagaatta
     4801 ggggaaggag atctttcaaa aaacaaacca gacaatttaa ctaaaatgga atctggtaaa
     4861 atctattctg attcagaaaa acaacaatat ctgttaaagg ataatatcat tctaagaaaa
     4921 ggctatgcac taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat
     4981 ggagtctata gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagcc
     5041 ctttcagaaa caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa
     5101 tctgtattaa tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt
     5161 aaaatgaacg ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct
     5221 aaacttgtat tatttggtaa ggatgataaa gaatacactg gagaggataa gttcaatgta
     5281 gaagctataa aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca
     5341 atggataaga actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa
     5401 ttttatttaa gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaat
     5461 gaatcggttg tagataatta tttaatctac ggagatttac acattgataa cactagagat
     5521 tttaatatta agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat
     5581 aaagcaaacg gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact
     5641 ggctatagcg atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat
     5701 gttaaacccg aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga
     5761 aaatctgtag tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa
     5821 gaacaacata tttatgtaaa tggaaaagaa tatacatcat ttgatgatat taaacaaata
     5881 acagacaaga cactaaacat taagattgtt gtaaaagatt ttgcaagaaa tacaaccgta
     5941 aaagaattca ttttaaataa agatacggga gaggtaagtg aattaaaacc tcatagggta
     6001 actgtgacca ttcaaaatgg aaaagaaatg agttcaacga tagtgtcgga agaagatttt
     6061 attttacctg tttataaggg tgaattagaa aaaggatacc aatttgatgg ttgggaaatt
     6121 tctggtttcg aaggtaaaaa agacgctggc tatgttatta atctatcaaa agataccttt
     6181 ataaaacctg tattcaagaa aatagaggag aaaaaggagg aagaaaataa acctactttt
     6241 gatgtatcga aaaagaaaga taacccacaa gtaaaccata gtcaattaaa tgaaagtcac
     6301 agaaaagagg atttacaaag agaagatcat tcacaaaaat ctgattcaac taaggatgtt
     6361 acagctacag ttcttgataa aaacaatatc agtagtaaat caactactaa caatcctaat
     6421 aagttgccaa aaactggaac agcaagcgga gcccagacac tattagctgc cggaataatg
     6481 tttatagtag gaatttttct tggattgaag aaaaaaaatc aagattaaga caaaagctat
     6541 agaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt tttgtgaaaa
     6601 cagccattta taactcaatt atttagttta cttcactatt gatactattt ggagttatta
     6661 atggacttag tttagataac taatgaattg attgaaaggg ttagtattga caatattggt
     6721 catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt gatgagacaa
     6781 tttcaaggaa ttaagtagcg tttggttgct taattccttt ttatttggaa ggaagttttt
     6841 cgtaaccgcc caataacgaa gtctattgga cggttacgat atgatcactg acttcgtcag
     6901 tcttatctac aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc ctagtttgct
     6961 ctttgatttt cattgagtat tagaacagaa aaatgcttgg agtatttgtt tgtgtattta
     7021 tttttatata acaaactata aacaaaacaa aaatataaaa aagagacaaa aaaagaacag
     7081 aaagtaatta acaacgattt catatagtgt tatacttatg gcataaagtt aatggaaaga
     7141 agggaaaacc ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa
     7201 gcggataatc aagaacaact ctttgatcag gtagcaagct tgttggaaga acgagaaatt
     7261 gtcactccta cttatcgtga agccttgatc acgcgtgaaa agtcatttcc aactggttta
     7321 gatatggaat ttctaggaaa ggacttgcca aatgtagcga ttcctcacac agatattgtt
     7381 cataatctag ctgagaaagt ggtggttgtt cgattagaaa aaccagtaac
//
LOCUS       DAVKZB010000088         7121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKZB010000088 DAVKZB010000000
VERSION     DAVKZB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.088"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            135..1397
                     /locus_tag="VXA80_001588"
     CDS             135..1397
                     /locus_tag="VXA80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV0354055.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(1690..2160)
                     /gene="nrdI"
                     /locus_tag="VXA80_001589"
     CDS             complement(1690..2160)
                     /gene="nrdI"
                     /locus_tag="VXA80_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV0354056.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(2238..2771)
                     /locus_tag="VXA80_001590"
     CDS             complement(2238..2771)
                     /locus_tag="VXA80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354057.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            complement(2823..4109)
                     /locus_tag="VXA80_001591"
     CDS             complement(2823..4109)
                     /locus_tag="VXA80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354058.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICCAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(4121..5767)
                     /locus_tag="VXA80_001592"
     CDS             complement(4121..5767)
                     /locus_tag="VXA80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0354059.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(5769..6383)
                     /locus_tag="VXA80_001593"
     CDS             complement(5769..6383)
                     /locus_tag="VXA80_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0354060.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            6518..7087
                     /locus_tag="VXA80_001594"
     CDS             6518..7087
                     /locus_tag="VXA80_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0354061.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
BASE COUNT         2124 a         1604 c         1271 g         2122 t
ORIGIN      
        1 ataacagtta tttcagattt tctttgtaaa ttttctatct tttttcaaaa tattcttgca
       61 tttttccaaa attttttgta gaatatagat tacatataca gattattctg aaaattcaaa
      121 aaggagtatt tatcatgtcg caagctattt ccttaaatca atcaacctgg gcaagcaagc
      181 taaaagcaat gggacctgga atcctaatgg caactgccgc tgttggaggt tcccacattg
      241 tatcctcaac tcaagctggc ggttcttacg gttggtctct acttctcttg gtcatcttag
      301 ccaatgtctt taaatatcca tttttccgtt ttggtgctga atacacagct gatactggaa
      361 agactttggt tgaaggttat gccgaaaaag gaaaactcta tctctggatt ttctttatcc
      421 tcaatgtctt ttcggctatg gtcaacacgg ctggtgttgc cattctgtgc tcagctatca
      481 tcgccagtgc cttcccaatg attggactta gcattactca gtggtccctc attctcgttg
      541 caatcatttg ggctatgcta ctctttggag gctacaaact tttagacggc atggtcaaat
      601 ggattatgtc tgccttaacc attgcgactg ttcttgcagt tatcattgcg gcggtcaagc
      661 atccagaata cagttctgat tttgtcgaga agacaccttg gcaaatggca gctctgccct
      721 tcatcgtctc cctcctagga tggatgccgg ctcctattga aatttcagcc atcaattcac
      781 tttggtcagc tgaaaagaga aagaccgtca actttaacac agaagacgct ctgtttgact
      841 ttaacactgg ttatattgga acagctatcc tagccgtctt ctttgtggca ctgggagcac
      901 tgattcagta tcctacaggg caggcggttg aagctgcttc agccaaatac atctctcaat
      961 tcgtgggcat gtatgcctct gttcttggcg aatggtcccg ttacttgatt acctttattg
     1021 ccttcctctg tatctttgga acagttataa ctgttatcga tggctattct cgcgttaatc
     1081 aggaatctct ccgactgcta atcagtcaaa aagaggacaa tcgtaaatct ttgaacatct
     1141 ggatgaccat cactgctatc atcggtatcg tcattatcaa gttcttcgct ggtcaggttt
     1201 caaccatgct ccgctttgcc atgattggct ctttcctgac aacacctttc tttgctcttt
     1261 tgaattacgc cttggtaacg cgtgaaaaca aaaatcttcc ttcttggctc aaacaccttg
     1321 ccattgcggg attgattttc ctctttggct tcgccatctt ctttatctac gcactcgcaa
     1381 tcggaaaagc agggtaaggg acaagcgcga gatgaagata aggtttcatt tcaagagaaa
     1441 attcagcaaa tatttctatg ataaaaagca taagaacaag gttttgaaga cctgaactta
     1501 tgctttttta cgttcttaaa gactgtttat actcaaaaaa cagttgaaca acttcaacca
     1561 cctcttataa gaactttata ctattcgaga atctcttcaa accacgtcag ctctatctgc
     1621 aacctcaaag ctgtgctttg agcaacctgc gactagcttc ctagtttgct ctttgatttt
     1681 cattgagtat taattctcct tttccaactc atacaaatct gcgataatag ctgcgacatg
     1741 tttgatatct tccagcatgc ctcgcatttc aaagtcagcc aatacaggga agccaaagcg
     1801 ttgactgtat tgcttggctg ttaggcagta ttggttatta aagttacgat ttcctgaccc
     1861 aaccacacca aaacacttac tagcattgtt accataggca ataaaatctc ccaccggtgt
     1921 cgtcaaaatc tcaacatctc cgttatccac gccattccca ccttcgagat aggtcggcaa
     1981 aaaagcgaca tagggatggt ccatttcata gaaatttttg ccttccttga ccaaatcctt
     2041 gatatgaatc ttttgaacct caatcccttt gtactgggac aagagatagt ctttcaagcg
     2101 cgtcacaaaa ctttcagtgt tgccactcaa actaatataa actaaagaaa ttgtcttcat
     2161 atgtacctcc atgattttat ctataatcaa aaaacaacta ccaagattgt atcttgatag
     2221 ttcttttttg tcaatactta ggtaagctct tctgtttcct cttcttctgc caaggctttc
     2281 tgttgtcgcc acatcttata gaagacaagg gctaggaaga gaacattgat gacgatatag
     2341 aaaatgctaa cagcttttga accaatactc actgtcaaac gcatgctctc atcttgaacc
     2401 aactgcacag cgtcttgtaa accaacatag ccataaatcg aaccaataat agctaacaaa
     2461 acatagccaa cgtaagtata gtttgcatat tgcaaatttt tacgaacaag gaagacaatc
     2521 gctacgacca ctaaaatagt agatagcaca atcaaagcaa cattaaaaat agaatgagat
     2581 gcttctgcta ctctataggt ataattaagc tggtcttcta attgctgagc attgacccct
     2641 gcaacttgtt tctgagcagc acgaagggct tctttactag gcaagggact cacaattcca
     2701 aacagagacg gaactgaaat aagggcagac aagattagca aaacccatag ataaattggt
     2761 ttctttttca tgataggacc tcctgataat attatagcac cttttgtaaa ggaatacaaa
     2821 tcttatagta tagtttctac ctgttttcgg taggcttttg gcgattttcc gcacaatttt
     2881 cggaaaactt tatagaaata tccaaggtta ctgtaaccaa cagcacaaca aatatcttcg
     2941 atgctgtcac tagttgaaag caagagctgc tgggcagcct taatacgttg tttatttagt
     3001 aactctgcaa aggtcgaatc ggtttcacgc ttaatcaact gccctagata gacaggattg
     3061 ataaagaggg ccttactgat atccttgagg gaaagctctt tttggtaatc acgaccaatg
     3121 acttccagca cactgaccac attttcattc atacggtatt gaccgaaaaa gctgatcaga
     3181 gtttccttga tataagaaac caattcatcg aaggattgaa tagcatgaat ggttttgaca
     3241 atgtccgtca tatcatcagc tttgagatgt tcaaataaat ggaaaacatc catgacaaac
     3301 tggataaaaa gctgtttagt aatcgaaaca cgaggtgtat tttcaagaac taccttctcc
     3361 aagagattta actcttctac gatttgattg agatttccct gaatgataac cctataaatc
     3421 ggttcgtaat aggcaaagag actctcagac tgctgcagat ttacagaacc gtaaaagagg
     3481 gttttctcag cattcagctt ccagcgttca aaatgttctt gataaggagc ttcaaaaggt
     3541 gtaacgacta aaccatctag ggggtggcca gaaatgaaaa tctgccagtc ttgacccaag
     3601 acatagtagg gaatggtgaa ggaaccttgt ttttccttgg atagacctat ccaccaattc
     3661 tccttatccc ctaaataact aacaaatcca gcctcgtcta attcttgact aagagtctga
     3721 cttttcttcc ctctctcgcc gagctgacct gcaatcttct ccagcagatt tcccagctct
     3781 accttatcaa caggcttgac caaatagtcc accacactaa ggttcattgc tctttttaca
     3841 taatcaaact cctgataacc tgagagcagg atataggcag catctggtaa gatctctttc
     3901 atctcccgaa tcatatcaag tcctgttttg tctggcatat tgacatcgga aatgatgaca
     3961 tcgacaggat tttcctgaac atattctaga gcttcatcgg catgactggc tgttgcgacg
     4021 acctccatat cccacttatc aaagggaatc aaacgcttca gaccttctgt caccatgtac
     4081 tcatcatcta ctaataatac tttatacatt ttctcccttt ctactcatct tgaattgtaa
     4141 tacgatactg aacaccggct tgctctgcag actctatagt aatggcatag cggtctccaa
     4201 aatagagcac aaaacgctcg tgtacattga caatcccgat agactgcctt tgatcactgt
     4261 agctggcttg gtgttcaaaa tatctctgac ttaatttttc tcggatattt gccaactttt
     4321 cagccgacat tcctctacca ttatcgacca ccaaaatttc cacaaaacca tcctgtttaa
     4381 gagccttgat gctaatcaca ttatctgtcc gcctgtggtc aacaccatgc gcgaaatagt
     4441 tttctaccag cggttgcaag gtaaacttgg gaatcttcat attctctaac tctggatcta
     4501 tcttgaaacc ataggcaatg gacttgggat agcgaaccat gcagagatag ctgtatttac
     4561 ggcaaaattc taattcctgt ttgaggaggg tctctctttc gtcggaaata ttgttacgca
     4621 agagactact gaattcataa atgatatctg ccaactcatc ttgactctgc ataactgcat
     4681 acatgcgcaa gaactccagc gtattataca taaaatgagg attgatttgc gcctgcaagg
     4741 ctcgcatatt ggcatctttt tgactaagct ctaactggta aatatcatgg atattctttt
     4801 ccaatcgatc caacatatca ttggtcgtct ccgcgattag gagtaattcc tgatctttct
     4861 cggaaatgtc aatccgacgc ccctcttcgc cttgagcaat gacttgaatg gattctacta
     4921 aatccactac ctgcttttgg taattagcaa aagtctgcct caaagtcaga taaagaatga
     4981 caataaagag aaggctcaaa cccacaatca aagcagagct agtcactgtt ccttgtaaaa
     5041 caaagttttt aggaactgcc acctgaacct gatatccatg agaagttaag ccaactagcc
     5101 aattttcact ctccttatca actgtctcac caatatgaaa aatctccgta tcaaaaggtg
     5161 aggtcactgt tactgccatc ggagtatgac ctctagtatt atcaatggca tggtataaaa
     5221 cagcaggatc caaggagatg taaatcactc ctaaatcttg atctgacact ggatctgaca
     5281 ccggaatggc aaaactattt cctgctggtt tgaaatcctc agccctgatt ttttctccac
     5341 tacgtttgtc tttagtagaa acatagactt ccttgtaatc ttgaaaagca atggccaccc
     5401 cagttacaaa atcatttcga acatagaggt catcaacatt ttcatacaga gagactgata
     5461 tataaggcga agctttccgc tctaattgcc agtaaaaata gtctggcatg ctaaggctaa
     5521 agtatttata gattccctcg atacgagctt gattttcaac taaagcctga gctatctggg
     5581 ttgactctct atggtaatat tcaatttcac tcactgttct agcagttaca cgctgggcta
     5641 ctctctgggc ttccttttca cgtgaatccc agtcagcgta agagagtaag attgcaaaac
     5701 tcgcaatgat aatgatcatc actgaactat aggttcttag aagcgtatgt agccaaaact
     5761 gcttcattct attttgtcgc caatttttca tggatgattt catagacagg ttccaacata
     5821 tcactgataa agaaatgcca catcccaatt cctacaaaaa atagcagggc aggcatataa
     5881 taaccaattg ctacaagtag cacagtccca aggagaacct tagccacagc tgctagactc
     5941 ataaagatgc caatcaagga taatttgaga ctatttcgat aggacaaggc aaaataaact
     6001 tgtaatttta aagatactgt ataagccaaa aagatcaggg caactactag gacattcaaa
     6061 agggtcgcaa tcaaatgaac aatggtctga tgaggcaatt gcaccaagag atacaaacca
     6121 taggtcaaaa ctagacccac acctaaaaag ctatagaaaa tcaggttgct tgagacaaaa
     6181 ttttgcttgt aaagagacca agcctccttc aaactgtatt cccgaaagct ataaccatgt
     6241 tctgcatata agctcatcaa ggtggcacta gccggcgcta gaccaaggat aatccctcct
     6301 gctaaagtta atagccaaaa gaaggctgtc gcaatcatcc ctaaaaagaa acgattaaag
     6361 acaaagtcta aaaatctacc catgatattc ccctaaaaat aactatgaaa ccattatatc
     6421 atatttcaag cgttttcaaa aacttctagt atccatttac aatcccacca aaccatgata
     6481 aaatgagaat tgtgaaaaaa ttatcaggag acaattcatg aagaaatcta tcttaactac
     6541 actgcttttt gcagttcttt acttcctctg catgggaatt ggtgtccttt tgggcaatct
     6601 ctttgaccaa actggaaaca tgttttatgc gcctgccttt actgcccttg tcggcggtag
     6661 cgtctatatg atcctagtcg caaaagttcc gcgctttgga gccattacca ctatcggcct
     6721 tgtcattgcc ctctttttct tgggaactaa acacggtgct ggttccttcc ttcctggaat
     6781 tatctgtggc ctcctagcag atggagtagc tcatttagga aaatacaagg acaaaacaaa
     6841 gaacttcctt tctttcatta ttttcgcctt tagtacaaca ggaccaatct tgcttatgtg
     6901 gattgcgccc aaagcctata tggctactct tctggcaaga ggaaaatccc aagaatatat
     6961 cgaccgtatc atggtcgctc caaaccctgg aactgtcctt ctatttatcg caagtattgt
     7021 catcggagcc ctagtgggtg ccttgattgg acaagccttg agtaaaaaat ttgcccagaa
     7081 aatctgatca gttaaaaaga gccacgcggc tcttttttat t
//
LOCUS       DAVKZB010000089         7045 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKZB010000089 DAVKZB010000000
VERSION     DAVKZB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.089"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            221..3571
                     /locus_tag="VXA80_001595"
     CDS             221..3571
                     /locus_tag="VXA80_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0354062.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDNDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIALDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3842..3919
                     /locus_tag="VXA80_001596"
                     /pseudo
     CDS             3842..3919
                     /locus_tag="VXA80_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4157..4627)
                     /locus_tag="VXA80_001597"
     CDS             complement(4157..4627)
                     /locus_tag="VXA80_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0354063.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4644..6917)
                     /locus_tag="VXA80_001598"
     CDS             complement(4644..6917)
                     /locus_tag="VXA80_001598"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV0354064.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYTNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2168 a         1198 c         1515 g         2164 t
ORIGIN      
        1 gtaaatttgc tgatttctca ggtcaaccaa agataactca aaaaccatta gaaaatcaga
       61 aatatatcct cctcccacta gccctccaaa atgagttcgc agactttgta gccctggtcg
      121 acaaatcaca atttgcttgt gagatagcta taaaagtgtg gagaaatagc ttgaaattta
      181 gtataatata gctaaactat ttgtttaaag tgagaaaaaa atgggaaatt ttagctttct
      241 tttaaaaaat gacgaatatg aatctttttc aaaaccttgc attgaagctg aaaatatgat
      301 tgctacatca actgtggcta ctgcctttat ggcgcgtcgt gctttggagc aggctgtcca
      361 ttggatatat agtcacgatt catatttaga agctccctat cgtgctactc tatcttcttt
      421 agtatgggat aatgatttta gggatatcgt agattctgaa ctccacaagc agatagttct
      481 gttgattcgg tggggaaacc atgctgctca tggtggtgaa attaaggaac gagaagcgat
      541 tttagcttta catcatttgt atcagtttgt taattttatc gattattgtt acagcaatga
      601 atttgtggag cgttattttg atgagaagtg cttaccactt tcagcaaaca tcaaataccg
      661 agaaactcca caatctatga taaagttaca agacagttta ccagaactgc ctgattttca
      721 tgaacagatg gctgctcagt ccgtagaagt tcaagagact tatactgaaa aacgtgagac
      781 tgcagcgcaa cggcaagatg tgcctttcca tattgaacaa ttatctgagg cagagacaag
      841 aaagctcttt attgatatcg atctccgttt agcaggatgg atatttgaag aaaactgtcg
      901 tgttgagata gcccttgatg gtctcaagca cggttcagga attggttact gtgactatgt
      961 actttatggt aaaaatggga aaattttagc gattgtggag gctaaaaaag cctctgtcaa
     1021 tccagaagta ggggaagtac aggtcaaaga atatgctgaa gctttggaga aacatatcgg
     1081 ctatcagcca atttgcttta ttacaaatgg gttgaagcac tatatacttg atggtccgaa
     1141 ccgccgccag attgcaggct tttactctca agaagaattg caattagtga tggatagacg
     1201 tcatcttcaa aaaccgcttg aggatatttc tagtaaaatt agggacgata tttccgggcg
     1261 tcactaccaa aaacatgcca ttgcaagcgt ttgtgaagct ttctctaatc atcgtagaca
     1321 ggcacttttg gttatggcaa ctggggcggg gaaaactcgt acagcagttt ctctagttga
     1381 tatcttatca cgtcataact gggtaaaaaa cgttctcttc ttagccgata gaacttcctt
     1441 ggttaagcaa gcctatgatt cgtttagaaa attactccca gatctttccg tttgtaactt
     1501 cttagaagat aaagaaggag ctcaatcaag tcgtatggtc ttttcaactt atccgaccat
     1561 gattggagcg attagtggtc aagaagaagt aaatcaacgc ccttttactg ttgggcattt
     1621 tgaccttatc ataattgacg aatctcaccg ttctatttat cagaaataca agtccatttt
     1681 tgattatttt gatgcaagaa ttgtaggctt aacagctact ccgcgtcaag atttagataa
     1741 aaacacctat ggattcttta atttggagaa tggggttcca acatatgcat atgatttgga
     1801 agaggctgtt aaagacggat atttagtagc ctatcattct atcgaaacca aactgaaact
     1861 acctacggat ggtctacatt atgatgattt gtccgaagaa gaaaaggaac attttgatag
     1921 caaatttgaa gacgatagct gtgaaaaaga tattgatggg agtgtattta attcctttat
     1981 tttcaataaa agtacagtag aaattgtttt aaatgaactc atgacaagag gaattcagac
     2041 agcctcgggt gatgaaattg gtaaaactat tatttttgct aaaaatcatg atcatgcgga
     2101 atatatcaga ggtattttta acaaccgcta tcctgaaaaa gggagcgact atgctcaggt
     2161 gattgattat agtattaagc attatcagac cttgattgat gattttaaaa ttaaggagaa
     2221 gtatcctcaa attgcgattt ctgtcgatat gttagataca ggtattgatg taccagaggt
     2281 tgttaattta gtcttcttca agaaagtacg ctctaaaact aagttttggc agatgattgg
     2341 tcgaggaacc cgtctatgta aagatttatt tggacctgag caggataagg aaaacttctt
     2401 ggtatttgat tatggggaca attttgatta ttttcgtgca gatccaagag atggagaggg
     2461 tcgtcacatt gtttcgctga ctcagcgttt atttaatatc aaagtggact tgattcgaga
     2521 acttcaggga ctccaatacc aagaagatca gtttgcgaga gcataccgtc agcagcttgt
     2581 ctcggaactt caaggtcgta tagagagctt aaatgagttg gacttcaggg ttcgtatggt
     2641 tttagataca gtttatagct ataggaaatt ggaaagttgg cagaatctaa ctgctgttac
     2701 aagtgaaacc attcaaaaaa atctctctcc gcttttattt gatgaagata aagaagatga
     2761 gatggcgagg agatttgatt tgtggttgct tcatattcag ttggggcaac tgacagctaa
     2821 atcttccact gttcatattt cccaagtgat gaagacggct agagctcttt ctgctattgg
     2881 caatatcccg caggtttttg agcaggctga aattatcagg aaagtacagg agcctgaatt
     2941 ttggaaagaa gttaacttgt ctgatttgga aaaaattcgt cttgctattc gagatttatt
     3001 acagtttttg gataaaacag accgtaaacc ctactatgtt aactttgaag atcgtatact
     3061 ctccactgtt cacgagacca cagcattttt gcaggtcaac gatcttcggt cttacaatga
     3121 aaaagttgag cattatttga aaactcatct ggatgaggag tccatttcta agctatacca
     3181 taataaaaag ttgacatctg atgatatgct tgcacttgaa aaattgcttt gggaaaaatt
     3241 aggtagtaaa gcagactacc aaagtcatta tgaaaataag gcaattccga gattggttcg
     3301 tgagattatt ggcttagata gagagtctgc caatcgtatt ttttctaaat ttttgtcgga
     3361 tgagaatctt aatgccaggc agatttcatt tgtaaaattg attgtagact acattgtaga
     3421 aaatggtttt ttagagacga aagtgttaac gcaagagccg tttaaatctt atggttctgt
     3481 tcaactactc ttccaacacc aactaccagt acttcgtaat attgttcaaa tcattgaact
     3541 tatcaataat cgagctggag aagcggctta aattctaaag tgattgccat gctgagactc
     3601 atttaaaatt aaaaagagta gaaatttatg ctatatatga gaagttttat taggaagaat
     3661 gtcatcgttt tcctagagta cagtatcagt tgttaagtgg ttgataaatt tcaaagtaga
     3721 tacttgtacc acgatgtttg ttgatcgagt tattaacaaa agagctactt tgattttaaa
     3781 gaaatagaaa acaaaaagcc gagcaagaat tcaattgcag gagaaaatga aataatactc
     3841 aatgaaaatc aaagagcaaa ctaggaaact agctgcaggc tgctcaaaac actgttttga
     3901 ggttgcagat aagactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac
     3961 tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggattg aagagatttt
     4021 cgaagagtat aaatcttcct aggataaagc aaaacgcata gtatcaaggg ttttcaacac
     4081 ttgatactat gcgttttctg atgttaaaga ctttctacca gtttttttaa gcataattgt
     4141 tagttgtagt catttattat tcttcaaaga aaaatggtgg ggcgaatttt ttcagttctt
     4201 caaagcactt ttgagcagta tctgcatctt cacagatgat aagacagaca tcattaccac
     4261 aaagggtagc gatagcgtca gggaagctca aagtatcaat gatagaacca aaggattgag
     4321 ccagtccagg aagggttttt agtaggactt ggtgttgaac tgggcgcatc cagacaaggg
     4381 cgtcttccat gtagagttcg agacgttttt cccattttga gatggaacca ttgttaagaa
     4441 cataataagc gctatcttct tcgcggactt ttgataggtt catatttttg atgtcgcgtg
     4501 agagggttgc ctgggttact tgaatgtcgt tcttagcaag aagggcttgc aactcagcct
     4561 gtgtatgaat cttgtttttt gtgataagag cgcgtataag ttggtggcgg tgttctgatt
     4621 tattcataat aatgtaactc cttttagcaa ggtaaggtaa gcatggactg agcgaggtcg
     4681 acagtcaagt ggtagtctgt attgtcacgg atggtgattt caaagtcagt agtatagagg
     4741 actaaacgga gagtgtctcc ttcttttagc ttgtaaatag ttggctgcag ttcaaattga
     4801 acatccatcc attcatctgc agtaatatcc tctactaaca gtaaatcatt tctattttgt
     4861 aaattaaggt aaccttttgt cacgactcgt tgtgcctctg gtctaaatgg caattcacag
     4921 agattttcca acatgtgata gcgaccgttg tcaatggttc tagcacttaa aatagctgga
     4981 taaggttgta ggtatttctt ttgcccaaat tctagcagtt gggcagataa gagccccttg
     5041 tttgtactgg atttgatacg aagattgagc tgagcgcgac cgtttaggtg gagatcttta
     5101 gtcacaggaa ggttaatagt aatctgattg gctttccctt gatagagctc tgtattgaag
     5161 gtttggtatg tcttaccata gcgctcaaaa tccttatctg ggtactggtt ttgaatagct
     5221 tgctcttctt gaccaagtga gaaggtttca cagttttctt gcccaccgaa gttatcaagt
     5281 gataaccaag tctgtggagc tgtattgtcc tgccagataa cagtaggaag ttgaaagtct
     5341 gtttcctgtc ctagtaattt cttggtcaat aaggcattta tggactcacg gaagtcaatt
     5401 gattgccaat tgttcatgta aacatgggca ccattatgga aaaagagatg cttgtttata
     5461 tgagtaggaa gagcatggaa catctggtaa acatgaagtg gtttgacatt ccaatcctga
     5521 gaaccatgag taaagacaac ctctgccttt actttatggg cattgagcag ataattgcgg
     5581 tcatgccaaa actgattgta gtccccagtt tttcggtcta gctgagcttt cactttttct
     5641 aagtcagctt ggtgagcttc attgccacgg atatagtcgc cagctaagag attacgagaa
     5701 taggttaact cagcaaggga gtcaaagtcc tcacctggat aaccacctgg gctagtcacc
     5761 agaccgtttt cacggtagta gttgtaccat gatgaaattc ctgcctcggc aatgataact
     5821 tctaaaccat cgactcctgt agtcgcaaga ccattggaca tggtacctag ataggaaagt
     5881 cctgttgtag caacttttcc gtttgaccaa tcagccttga cttgacgctg gcgcgtgtga
     5941 tcagtaaagg cacggcaacg gccgttaagc caatcgatga catttgtata agcctcgatt
     6001 tgctggtagt ctccattagt catgaaacct gtcgagtctt tggtaccaac acctgagaca
     6061 tagagattgg caaagcctct cggaaggaag tagtcgttta gtgtatagct agagttgatg
     6121 tgagttagct tttcctcagc ctctgctata agctcagctt taccttgggg ttggacgaga
     6181 tttagttgag gtttctctag ctcaatcttg tgaggaagct taacctcaag ctcgccctcc
     6241 atcttgtaga gagccttgtc actagccttg tcattggttc cctgatgata agggctggct
     6301 gtcatgatgg cagggatttt tccatcaaaa cgagggcgaa taatgctaac ctttactagg
     6361 tctgatagcc ctttttggtc agtatcgaca cgagactcaa cgtaaacgac ttcacgaatg
     6421 acatcctggt tagaaaaagt agccaaactc ttgccgttaa agtagtggta gtcattatcc
     6481 tccggaataa gaccatcact aacaagttgg tcgataagag tatttccttt tttggtgcga
     6541 gtattgagta actgatagag attttcaatc aagtcaccat atataatggg aaatccagtt
     6601 tctttacgaa aaacgtcact atcttcgaag tcaaccaaat aagaaaagcc taaaagttga
     6661 aaagcaacag tataaaaaat atctgctgtc agttcatctt ctgattgaaa aaatgtcagc
     6721 aggtctgttt ttttatcagc tgctaggata gaaagtgggt agttggtgtc ttgataagtg
     6781 aaaaagaaac gacgtaaaaa ggtttcaagt gagtctttgt gattggctgt attttgtaaa
     6841 tcaaagccac atttttttag ttcagataag acattttctt ttggaaaatt gatataacta
     6901 tattgattaa aacgcataga acctccatat agaatgacag ttaaggttat tatatcaaaa
     6961 aaaaagcaga aagggaattg ttaacttcaa aaggaaataa tccaataaaa atgaataaag
     7021 tactaaattc aatatagaga acaga
//
LOCUS       DAVKZB010000090         7030 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKZB010000090 DAVKZB010000000
VERSION     DAVKZB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.090"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            33..1355
                     /locus_tag="VXA80_001599"
     CDS             33..1355
                     /locus_tag="VXA80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0354065.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1357..3243
                     /locus_tag="VXA80_001600"
     CDS             1357..3243
                     /locus_tag="VXA80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV0354066.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDGFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GLHL"
     gene            3411..3755
                     /locus_tag="VXA80_001601"
     CDS             3411..3755
                     /locus_tag="VXA80_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0354067.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3765..5177
                     /gene="lacG"
                     /locus_tag="VXA80_001602"
     CDS             3765..5177
                     /gene="lacG"
                     /locus_tag="VXA80_001602"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0354068.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5246..6925
                     /locus_tag="VXA80_001603"
     CDS             5246..6925
                     /locus_tag="VXA80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0354069.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QPISFVLLSLMLVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
BASE COUNT         2131 a         1001 c         1500 g         2398 t
ORIGIN      
        1 atttataaaa aataaaaaaa ggagttccgg tgatgaacaa tattttagcg tttttagaaa
       61 caaaagtcgc tccgtttggt gaaaaagttg gcaaccaacg gcatttgaaa gctattcgtg
      121 aaggatttat gatggcaatg cctttgattt tagtcggatc tttatttctt attctaatca
      181 gttggcctca agaggatttt acaaattggc tgaatagtgt tggattgcta agtatcttga
      241 caactatgaa tcagtcaaca gtagcgatta tctccttggt cgcttgtttc ggtattgcct
      301 acaggttgtc ggaaggatat ggtacagatg gtccgtcggc agggatcata gccttatcca
      361 gttttgtatt gatggcacct cgtttttcga gtatggttta tgataaaaat ggggagcagg
      421 tcaagcagtt atttggcggc gcaataccat tttctagcct gaatgcatct tctttgttta
      481 tggcgattac tattggattg gttacagcag agatttatcg tatgtttatc cagcgcggaa
      541 ttacgataaa aatgccaagt ggtgtcccag atgtggtaag taaatcattt tcagctcttt
      601 tacctggctt tactactttt gttttgtggg ctttggtctt aaaaggtctt gaagcggcag
      661 gagttgcagg aggtctcaac ggactcctag gtgcaattgt tggaacaccg cttaagttaa
      721 ttgcaggaac gcttccaggt atgattctat gtgttattgt aaactcattc ttttggttct
      781 gtggagttaa tgggggacaa gttttaaatg cttttgtaga cccagtttgg ttacaattta
      841 ctacagaaaa ccaagaagct gtggctgcag gacaaacact ccaacacatt attacattac
      901 cgtttaaaga tttatttgta tttattggtg gcggtggagc gactattggt cttgcgattt
      961 gtctcttcct atttagtaag agtcgtgcga ataaaacatt aggtaagcta gctattatac
     1021 cgtctatttt taatatcaat acagctattc tatttacgtt tccaacagtt ttaaatccga
     1081 ttatgctgat tccgtttatt gctactccta caatcaatgc cttgattacc tatgtatcaa
     1141 tggctgtagg attagtaccc tatacaacag gtgtaatcct tccgtggaca atgccaccga
     1201 ttataggagg cttccttgca acaggggcta gttggcgagg agctctatta caagttgttt
     1261 tgattttggt ttctgtagca atttattatc cattcttcaa aattgcagat aaacgcaatc
     1321 ttgaaaaaga aaaagctact gttggaggga aataagatgg ttatcagagt atttgatcaa
     1381 cagaaaaata cttattctag ctttgcctta gaggaattaa gttactatat gaatcgggtc
     1441 tttaagacta acatagagct tgtcgaggag aaggaagcgg atatttttgt aggattagtc
     1501 aataaagagg acagaaaaga ccatgttctt atctcattag acaagggtaa ggggagaatt
     1561 gagtctaata caattgtagg tttacttatt ggaatttacc gaatgtttca tgaatttggg
     1621 gttgtgtata ctagaccagg gcgcagacat gactttgttc cagagttacg atttgaagat
     1681 tttttagata aacagctatc tatagatgaa acagccagtt actatcatag gggagtatgt
     1741 atagagggag cggattcatt tgaaaatata ctagatttca ttgattggct acctaagatt
     1801 gggatgaaca gttttttcat ccagtttgaa aatccttact cttttttgaa acgttggtat
     1861 gaacatgaat ttaatccata tctaaataaa gaacaatttt caaatgaatt agtacaagaa
     1921 ttgagtgata ggttggataa agaattgcaa aaaagaggtc ttattcatca tcgtgttggt
     1981 catggatgga caggtgaagt tttaggttac tcttcaaaat ttggctggga atcaggtctt
     2041 agtatttcag aggagaagaa accctatgtc gctgaaataa acgggaaacg agaattgttt
     2101 aatacggctc cgattttaac cagcctggat ttttcaaatc cagatgtagc tgataagatg
     2161 gtagaaatta tcaaggatta tgccaagaaa agacctgatg ttaactactt acatgtatgg
     2221 ttgtcggatg ctcgtaataa tatttgtgaa tgcgaaaact gtagacaaga attggtttcg
     2281 gatcagtata ttcgtattct caatcaattg gatagggctt taacgagtga gggattagat
     2341 acaaagattt gttttctgct ttatcatgag ttgttatggg cgcctcagaa agaaaaatta
     2401 gataatcctg aacgctttac catgatgttt gcaccgatta caagaacatt tgaaatgagt
     2461 tatgcagatg tagattttga caattccata cctacgccta aaccttatat gcgtaataaa
     2521 attatacttc cgaattctct tgaggaaaat ttatcttatc tttttgagtg gcaaaaagca
     2581 tttaaaggag atggtttcgt atatgactat cctttagggc gtgctcatta tggcgattta
     2641 ggctatatga aaattagtca aactatttac agagatgtat cttatctttc caacctacat
     2701 ttgaacgggt acatttcgtg tcaagaatta cgtgccggat tccctcataa ttttcctaat
     2761 tatgtcatgg ggcaaatgct ctggaagaag acaagaagtt atgaagaatt gattgaagaa
     2821 tacttttctg ctttgtatgg ggaaaattgg cagtctgttg ttgaatattt agaaaaatta
     2881 tccagttatt cctcttgtga ttattttaat gcaattggca gccgtcaaag tgatgtttta
     2941 gcgaatcatt attatatagc ttacaatcta gctgataatt ttttaccaat tattgaggaa
     3001 aatatttcta agttattaaa tagtcaaaag gatgaatgga aacagctcag ttatcatcgt
     3061 gaatatgttg ttaagatggc gaaggcttta tatcttcaag caactggaaa aacaaggcaa
     3121 gctcaagatg aatggagaaa tgtgttgaat tatatccgtg ggcacgaatt gctatttcaa
     3181 tctaatttgg atgtttatcg tgtaattgaa gtagcaaaaa attacgctgg tttacactta
     3241 taaatcataa gtatagaaaa tgaactaagg tattcagaga agattgatcc taaatattat
     3301 gaaatttaag gatttttaag atatttaggg tcaactttct atttatatcg tagcgaagtc
     3361 attttaataa tgatgtgtaa aagatggatc aagattgagg aggaagaaag atgaaatcaa
     3421 aagaagaaat aaatatgctt ggttttacaa ttgtcgctta cgcaggagat gcaaggtcag
     3481 atttgatgga tgctttggcg tttgcgagag atggatattt tgaacaggca agagaattgg
     3541 ttgagtctgc aaacgactca atagtgtctg cccatcgaga acagactaat ttattagcgg
     3601 aggaggcata tggagataat tttgaagtga gctttattat gattcatggt caagatactt
     3661 tgatgacaac gatgctattg tatgatcagg taaagttttt tattgatgaa tatgaacgaa
     3721 ttcgaaagat tgaagaacat attggtttgc aatgaggatt agtcatggaa aatttacagg
     3781 ttaaagcctt accgaaggag tttttattag gagctgctac cgctgcttat caagtagagg
     3841 gtgcaactag ggtagatggc aaaggaacaa atatgtggga tgtttatttg caagaaaaca
     3901 gtccgttctt accagatccg gctagtgatt tttattatcg ttacgaagag gatatagctt
     3961 tggcggcaga acatggtttg caggctttgc gtttatctat ttcttgggtt cgtatatttc
     4021 ctgatataga tggggagcct aatatattag ctgttcgtta ttaccataga gtctttcagt
     4081 cttgcttaaa acataatgtg attccgtttg tttctttaca tcattttgat tcgcctcaga
     4141 aaatgttaga aacaggggat tggttgaata gagagaatat tgatcgtttc atacgatatg
     4201 ctcgcttttg tttccaagaa tttacagaag tcaagcattg gtttacaatc aatgaactga
     4261 tgtctcttgc tgcaggtcaa tatataggag gtcagtttcc tccaaatcat cattttcaat
     4321 tatctgaagc aattcaagcg aatcataata tgttgttggc gcatgctctt gcagtcctcg
     4381 aatttcatca attagggatt gagggaaagg taggttgtat tcatgcttta aagccaggct
     4441 atcctattga tgggcaaaaa gaaaatattt tggcagctaa acggtatgat gtttataata
     4501 ataaatttct attagatgga acttttttgg gctactacag tgaggacacg ctttttcact
     4561 tgaatcaaat attggaagct aataattcta gctttattat tgaagatggt gatttagaaa
     4621 ttatgaagag agctgcacct cttaatacga tgtttgggat gaattattat cgttcagaat
     4681 ttattcgtga atacaaaggt gaaaatagac aagaatttaa ttcaacagga ataaaaggac
     4741 agtcttcttt taaattaaat gctctaggtg aatttgtaaa aaaacctggt attccgacaa
     4801 cagattggga ttggaatatt tatcctcaag ggttatttga tatgttgctt cgtatcaaag
     4861 aagaatatcc tcaacatccg gtcatttatt taactgaaaa tggtacagcc cttaaagaag
     4921 ttaagccaga gggcgagaat gatattattg atgacagtaa gagaatccgt tatattgagc
     4981 aacatttaca caaagtttta gaggctcgag atagaggagt caatattcaa ggctatttta
     5041 tatggtcttt gcaagatcaa ttttcttggg cgaatggcta caataagcga tatggtcttt
     5101 tctttgttga ttatgaaaca cagaagagat atattaagaa aagtgctctt tgggtaaaag
     5161 ggctaaaacg gaattaaggt tagcgatttg actgatgttt aatatgtttt aaatatgagg
     5221 ttgaattttt tataggagga gttttatgga taagctagtc gctgccattg aaaagcaaca
     5281 agggaaattt gaaaaaattt ctactaataa ctatatgatg gctattaaag atggattcat
     5341 tgctactatg cctttaatta tgttttcaag ctttttgatg attattatta tgattcctaa
     5401 aaatttcgga gtagagttac cgagtccagc tattgtctgg atgagaaaag tgtatatgtt
     5461 aaccatggga gttttgggta ttattgtttc agggactgtt ggaaagtcat tagttggaaa
     5521 tgttaacaga aaaatgcctc acggaaaggt aataaatgat atttctgcaa tgttggcagc
     5581 catatgtagt tatctggtat taactgtaac gcttgtagtt gatgagaaga cgggatctac
     5641 aagtttgtcg acaaactatt taggatctca aggattgata acttcgtttg tcagtgcctt
     5701 tattactgta aatgtttacc gattctgtat taagcgagac attactattc atttacctaa
     5761 ggaagttcct ggggctatat cacaagcttt tagagatatt tttccttttt cttttgtttt
     5821 acttattagt ggtttgttag atattgtatc tcggtttagt ttagatgttc cttttgccca
     5881 agtatttcaa caactattga ctcctatttt taagggggca gaatcatatc ctgctatgat
     5941 gttgatttgg tttatgtgtg ctttgctttg gtttgttgga attcatggac catctattgt
     6001 cttacctgct gttacagctt tacaactgag caatatggaa gagaatgctc aacttcttgc
     6061 aaatgggcag ttcccttatc attctttaac gcctaatttc ggaaattata tcgctgctat
     6121 tggaggaacg ggggctacct ttgttgtacc atttattttg attttcttta tgcggtctaa
     6181 acaattaaaa tcggtaggta aagctacaat tactcctgtt ttatttgcgg taaatgaacc
     6241 tcttctattt ggtatgcctg ttattttgaa tccctatctt tttgtccctt ttttgatgac
     6301 tccaccagta aatgtatttc taggaaaggt atttattgat ttctttggaa tgaatggatt
     6361 ttatatccag ttaccttggg cttttcctgg tcccttggga ttgttaattg gaacgaattt
     6421 tcaacctatc tcctttgtgc ttttatcttt gatgttagtt gtcgacatat tgatttattt
     6481 gccattctgt agagcgtatg atagacagtt actgatgaaa gaagatgttg caagctcaaa
     6541 tgatattatt ttagaggagg atacaagtga aataatgcct ggtgagatag atgtaataaa
     6601 aagtaaggag ttgaaagtac tggttctttg tgcagggtct ggaacaagtg cgcaattagc
     6661 caatgcaatt aacgagggcg ctcaattagc agaagttaga gtgattgcga attcaggagc
     6721 gtacggagct cattatgata ttatgggtgt ttatgattta attattctgg ctccacaagt
     6781 tcggagttat tatagagaga tgaaggtgga tgcagaaaga ttaggtattc agatagttgc
     6841 taccagagga atggaatata ttcatttaac aaagagtcca agtaaatcct tacaatttgt
     6901 attggagcat taccaagctg tgtagtaagt ttttccatct tttatttgag taaggatttt
     6961 gcttacagat aggcttggat ttaaaaacgt tccccctttt ttaatataag aatccctctt
     7021 tcacaattgt
//
LOCUS       DAVKZB010000091         6892 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKZB010000091 DAVKZB010000000
VERSION     DAVKZB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.091"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            73..1350
                     /locus_tag="VXA80_001604"
     CDS             73..1350
                     /locus_tag="VXA80_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354070.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1363..2010
                     /gene="vex2"
                     /locus_tag="VXA80_001605"
     CDS             1363..2010
                     /gene="vex2"
                     /locus_tag="VXA80_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV0354071.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2062..3441
                     /gene="vex3"
                     /locus_tag="VXA80_001606"
     CDS             2062..3441
                     /gene="vex3"
                     /locus_tag="VXA80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV0354072.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3621..3704
                     /gene="pep27"
                     /locus_tag="VXA80_001607"
     CDS             3621..3704
                     /gene="pep27"
                     /locus_tag="VXA80_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV0354073.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            3753..4409
                     /gene="vncR"
                     /locus_tag="VXA80_001608"
     CDS             3753..4409
                     /gene="vncR"
                     /locus_tag="VXA80_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV0354074.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4406..5734
                     /gene="vncS"
                     /locus_tag="VXA80_001609"
     CDS             4406..5734
                     /gene="vncS"
                     /locus_tag="VXA80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV0354075.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            5875..6756
                     /locus_tag="VXA80_001610"
     CDS             5875..6756
                     /locus_tag="VXA80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV0354076.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
BASE COUNT         2116 a         1343 c         1508 g         1925 t
ORIGIN      
        1 tttttttttt cgtatttcat aaacatttca tatttgggtt ttataatagt cttacaaata
       61 tggaggtgac aaatgaatcc aatccaaaga tcttgggctt atgtcagcag aaagcgactg
      121 agaagtttta ttttatttct gattttattg gtcttattgg ccggaatttc agcctgtttg
      181 actctgatga agtccaacaa aacagtagaa agcaatcttt ataaatcact caatacatct
      241 ttttctatta agaagataga gaatggtcag acattcaagt tgtcagacct agcatctgta
      301 agcaagatta aggggctgga aaatgtctct cctgaacttg agacggtcgc aaaactaaaa
      361 gacaaggaag cagtgactgg cgagcagagc gtggagcgtg atgatttatc agctgcagac
      421 aataacttgg ttagcttaac ggctcttgag gattcatcca aggatgtaac ctttaccagt
      481 tcggctttca atctaaaaga agggcgacac cttcaaaaag gggattccaa gaaaatcctt
      541 atccacgaag aattggctaa gaagaacggt ctttcacttc atgacaagat tggcttggat
      601 gctggtcagt ctgaatctgg aaaaggacaa acagtagagt ttgagattat tggcatcttt
      661 tctggtaaaa aacaagagaa attcacaggc ttgtcttctg acttcagtga aaatcaagtc
      721 tttacagact atgaaagtag ccaaaccctt ttgggcaata gtgaagctca agtcagtgca
      781 gcacgcttct atgtagaaaa tcctaaggaa atggacggac tcatgaagca ggtagaaaac
      841 ttggccttgg aaaatcaagg ctaccaagtc gaaaaggaaa acaaggcttt tgaacaaatc
      901 aaagactcag ttgcaacttt ccaaaccttc ctgaccatct tcctttatgg gatgttgata
      961 gcaggagctg gagccttaat tctggttttg tctctctggt tgagagaacg ggtctatgaa
     1021 gtggggattt tacttgcact tggaaaaggc aagagctcga tcttcctaca attctgttta
     1081 gaggtagttt tggtatctct tggagctttg cttccagcat ttgttgcagg aaacgcaatc
     1141 acaacttacc tactccaaac tctactagca agtggagatc aggcaagctt acaagataca
     1201 ctagccaaag caagcagttt atcaactagc atcttatctt ttgcagaatc ctatgttttt
     1261 ctagttctgc ttagttgctt atctgtagcc ctttgtttcc tattcttatt tagaaaatca
     1321 ccgaaagaaa ttttatcatc tattagttaa gaaggagaaa tcatgacttt attacaatta
     1381 caagatgtta cctaccgtta taagaatact gctgaagcag tcctatatca gatcaattat
     1441 aattttgaac ccggaaaatt ttacagtatt attggggagt caggagcagg aaaatccaca
     1501 ctcttgtccc tacttgctgg tctagatagt cctgttgaag gttctatcct ttttcaagga
     1561 gaggatattc gtaagaaggg ctattcttac catcgcatgc accatatttc cctggtcttt
     1621 caaaattata acttgataga ttatctttct ccgctggaaa atatccaatt ggtcaacaaa
     1681 aaggcaagca aggatacact tcttgagctt ggtttggatg aaagtcagat caagcggaat
     1741 gttctccagt tatcaggtgg tcaacagcaa cgtgttgcca ttgctcgcag tttggtctca
     1801 gaagctccag ttattctagc tgatgagcca acaggaaatc tggatcctaa aactgctgga
     1861 gatattgtcg aactactcaa atcacttgcc cagaaaacag gtaaatgtgt gattgtcgta
     1921 actcacagta aagaagtggc acaagcgtca gatattacac ttgaattaaa ggataagaaa
     1981 ctgactgaaa cgcgcaatac tagtaaataa tttgagctta ctttaataga atgattaaaa
     2041 caaaatctag aaagggaatc tatgttacac aacgcatttg cctatgttac aaggaagttt
     2101 ttcaaatcga ttgtcatctt cctgattatt ctcctcatgg cgagcttgag tttggtcggc
     2161 ttgtcaatca agggagctac tgccaaggct tctcaggaga cctttaaaaa tatcaccaat
     2221 agcttctcca tgcaaatcaa tcgtcgcgtc aaccaaggaa cgcctcgcgg tgctgggaat
     2281 atcaagggtg aagacatcaa aaaaatcacc gaaaacaagg ccattgagtc ttatgtcaaa
     2341 cgtatcaacg ctatcggaga tttgactgga tatgacctga ttgaaacgcc agaaaccaag
     2401 aagaatctca ctgctgatcg tgccaaacgt tttggaagta gcttgatgat tacaggtgtc
     2461 aatgactcct ctaaagaaga caagtttgtc tctggttctt ataaactagt cgaaggagag
     2521 cacttaacca acgacgacaa ggataaaatc ctcttgcaca aggacttggc agccaaacac
     2581 ggctggaaag taggggacaa ggttaaactg gactctaata tctacgatgc agataatgaa
     2641 aaaggagcca aggaaacagt tgaagtgaca atcaagggac tctttgatgg tcataataag
     2701 tcagcagtaa cctactcaca agaactttac gaaaacacag ctattacaga cattcacact
     2761 gctgcaaaac tttatggata cacagaagac acagccattt atggggacgc aaccttcttt
     2821 gtaacagcag acaagaactt ggatgatgtt atgaaagagt tgaatggcat cagtggtatc
     2881 aactggaaga gctacacact cgtcaagagc tcctctaact acccagctct tgagcaatct
     2941 atctctggta tgtacaagat ggccaacctc ctcttctggg gtagcttgag cttctcagtt
     3001 ctcctccttg ccctcttgct cagcctttgg atcaacgccc gtcgcaagga agtgggaatt
     3061 ctcctctcta tcggcctcaa gcaggcaagt atcttgggtc aattcatcac cgaatctatc
     3121 ttgattgcta tccctgctct agtttctgct tacttcctag ctaattacac tgcccgtgca
     3181 attggaaaca ctgtccttgc caatgtgact tcaggtgttg ccaaacaggc tagtaaggcg
     3241 gctcaagcct ctaaccttgg tggtggtgca gaagtagatg gctttagcaa gaccttgtcg
     3301 agcctagaca tttctattca gacatcagac tttatcatca tttttgtcct tgccttggtt
     3361 ctagtggttc tcgttatggc gcttgcttca agcaatctcc ttagaaaaca accaaaagag
     3421 ctcttgctgg atggtgaata aatttgaaaa aatgagtctg gaataaagat tgcatcttgt
     3481 gtttctattc aagaatagtg gataggaatg gctatttaac aattcaaaat aaatccgaaa
     3541 gcagtggtga aaatcattgc tttcagttgt tttctttgta ctttattgct taaatataat
     3601 atactaaagc tatggaattt atgagaaagg aatttcacaa cgttttatct agtgatcagt
     3661 tacttacaga caaaaggcca gcaagagact ataatagaaa atagagtagg tatttattct
     3721 aagaaaaata aaaaatagag agcagttaaa gtatgaaaat tttaattgta gaagatgaag
     3781 agatgatccg tgagggggtc agtgattatt tgacggattg tggctatgaa actattgagg
     3841 cagcggacgg tcaggaagct ctggagcaat tttctagcta tgaggtggcc ctggttttac
     3901 tggatatcca gatgcccaag cttaacggct tagaagttct agctgagatt cgtaaaacca
     3961 gtcaggttcc tgtcttgatg ttgacagctt ttcaggatga ggaatacaag atgagtgcct
     4021 ttgcctcttt ggcagatggc tatctggaaa aacctttctc cctctccctc ttaaaagtga
     4081 gggtggacgc gattttcaag cgctactacg atacaggacg aatcttttct tacaaggata
     4141 ccaaggtgga ctttgaaagc tacagtgcaa gcctcgcagg tcaagaagtg cctatcaatg
     4201 ccaaagagtt ggaaattctg gactatctag tgaaaaatga aggccgggcc ttgactcggt
     4261 ctcagattat cgatgccgtc tggaaagcga cagatgaggt tccctttgac cgtgttattg
     4321 atgtttatat caaggaattg cggaaaaagc tagacttgga ttgtatcctc actgtgcgca
     4381 atgttggtta taaattggag cgaaaatgaa acgaacaggt ttatttgcaa agatatttat
     4441 ctataccttc tcgatattta gtgttctggt tatctgcctt catttagcta tttattttct
     4501 ttttccttcg acttatctga gtcatcgtca ggaaaccatt ggtcaaaagg caacagccat
     4561 tgctcagtcc ctagaaggga aagataggca gagtatcgag caagtgttag acttgtattc
     4621 ccagactagt gatatcaagg ggaccgtcaa aggtgagatg accgaggaca agttagaagt
     4681 caaggacagt cttcctctgg acacagaccg ccagacaacc tctctcttta ttgaggagcg
     4741 cgaggtgaaa acgcaagacg gtggtactat gattctccag tttctagctt ccatggattt
     4801 acaaaaggaa gcggagcaaa tcagtctcca gtttcttccc tataccttgc tggcctcctt
     4861 tctgatttcc ctcttggtgg cctacatcta cgctcggact attgttgcac cgattttgga
     4921 aattaagcgg gtgacccgtc ggatgatgga cctggattcc caagtgcgat tgcgcgtgga
     4981 ttctaaggat gagataggta atctcaagga acaaatcaat agcctctacc agcatctctt
     5041 gactgttatt gcggacttgc atgaaaagaa tgaagccatt ctccagctgg agaagatgaa
     5101 ggtcgaattc ctacgaggag cttctcatga attgaaaaca ccgctggcta gtttgaaaat
     5161 cctaatcgaa aatatgagag agaatatcgg tcgttataag gatagagacc agtatctggg
     5221 agttgccttg gggattgtgg atgaactcaa tcaccatgtt ctgcagatac tttccctctc
     5281 ttctgtgcag gaattgcgag atgataggga aacaattgac ctcctccaga tgacgcaaaa
     5341 tctggtcaaa gattatgcct tgctagccaa ggaaagagag ctccagatag acaatagttt
     5401 gacccatcag caggcttatc taaacccatc agttatgaag ttgattcttt ctaatctcat
     5461 cagcaatgcc attaagcact ctgttccagg tggcttagtt cgaattggag aaagagaagg
     5521 agaacttttt atcgaaaata gctgtagctc agaggaacaa gaaaaactag cccagtcttt
     5581 ttctgacaat gctagtcgca aggtcaaggg gtctggtatg gggctctttg tggttaagag
     5641 tctattagaa catgaaaaat tagcttatcg tttcgagatg gaggagaatc gtttaacctt
     5701 ctttatagct tttccaaaag tcgcccaaga ctagggagag aaagggttta catagatgga
     5761 gttagaagaa aatcaatcga aactgcggga aaaactagat ttttttggca aaaagtgata
     5821 aaatgaacaa tgtaaatggg atgacccata aaaatataca ggaggcctga taaaatggca
     5881 atcgtttcag cagaaaaatt tgtccaagca gcccgtgaca acggttatgc agttggtgga
     5941 tttaacacaa acaaccttga gtggactcaa gctatcttgc gcgcagcaga agctaaaaaa
     6001 gctccagttt tgatccaaac ttcaatgggt gctgctaaat acatgggtgg ttacaaagtt
     6061 gctcgcaact tgatcgctaa ccttgttgaa tcaatgggta tcactgtacc agtagctatc
     6121 caccttgacc acggtcacta cgaagatgca cttgagtgta ttgaagttgg ttatacttca
     6181 atcatgtttg acggttcaca ccttccagtt gaagaaaacc ttaaattggc taaagaagtt
     6241 gttgaaaaag cacacgctaa aggtatctca gtagaagctg aagttggtac tatcggtggt
     6301 gaagaagacg gaatcatcgg taaaggtgaa ttggctccaa tcgaagacgc taaagcaatg
     6361 gttgaaactg gtatcgactt cttggcagct ggtatcggta acatccacgg tccttaccca
     6421 gtaaactggg aaggtcttga ccttgaccac ttgcaaaaat tgacagaagc tcttccagga
     6481 ttcccaatcg tattgcacgg tggatcaggt attcctgatg agcaaatcca agcagctatc
     6541 aaacttggtg ttgccaaagt taacgttaac acagaatgcc aaatcgcatt cgctaacgca
     6601 actcgtaaat ttgctcgtga ctacgaagca aacgaagcag aatacgataa gaaaaaactc
     6661 ttcgacccac gtaaattctt ggctgacggt gtaaaagcta tccaagcatc ggttgaagaa
     6721 cgtatcgacg tattcggttc agaaggtaaa gcataatcta gctgaataat acagataaaa
     6781 cctgcctgtg agggtgggtt ttttgatttc aagctactcg ttttagtata gtaaactgaa
     6841 tctagactag cacaatgcta cttctatagc gttttgaaac tagaatagga ta
//
LOCUS       DAVKZB010000092         6826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKZB010000092 DAVKZB010000000
VERSION     DAVKZB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.092"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..372
                     /locus_tag="VXA80_001611"
     CDS             <1..372
                     /locus_tag="VXA80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354077.1"
                     /translation="KVSGRRYQRISLVAGLTNGELIAPMTYEETMTSDFFEAWFQKFL
                     LPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHL
                     KRVLPSCNTFYEALLSCSCFN"
     gene            433..1395
                     /locus_tag="VXA80_001612"
     CDS             433..1395
                     /locus_tag="VXA80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0354078.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1572..2579
                     /gene="gap"
                     /locus_tag="VXA80_001613"
     CDS             1572..2579
                     /gene="gap"
                     /locus_tag="VXA80_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV0354079.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2646..3482)
                     /locus_tag="VXA80_001614"
     CDS             complement(2646..3482)
                     /locus_tag="VXA80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0354080.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3608..5803
                     /locus_tag="VXA80_001615"
     CDS             3608..5803
                     /locus_tag="VXA80_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0354081.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTSAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5856..6008
                     /gene="rpmG"
                     /locus_tag="VXA80_001616"
     CDS             5856..6008
                     /gene="rpmG"
                     /locus_tag="VXA80_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0354082.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6018..6194
                     /gene="secE"
                     /locus_tag="VXA80_001617"
     CDS             6018..6194
                     /gene="secE"
                     /locus_tag="VXA80_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV0354083.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6249..6785
                     /gene="nusG"
                     /locus_tag="VXA80_001618"
     CDS             6249..6785
                     /gene="nusG"
                     /locus_tag="VXA80_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV0354084.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2113 a         1197 c         1483 g         2033 t
ORIGIN      
        1 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgag
       61 ttaatcgctc cgatgactta cgaagagacg atgacgagcg acttttttga agcttggttt
      121 cagaagtttc tcttaccaac attaaccaca ccatcggtta ttattatgga taatgcaaga
      181 ttccatagaa tgggtaagtt agaactttta tgcgaggagt ttgggcataa acttttacct
      241 cttcctccct actcacctga gtacaatcct attgagaaaa catgggctca tatcaaaaag
      301 cacctcaaaa gggtattacc aagttgcaat accttttatg aggctctttt gtcttgttct
      361 tgtttcaatt gactatagtt cacggataca gttgggaaag aagctattaa acatttggag
      421 agtccttttg ggatgaacat gtcaaatatt actatagatg ggcaagaaat ggatctgagt
      481 caaacgatgc cttcttatgc tcaagtagtg agtctttatg aggatacaag catctctgtt
      541 agtagtaatg agacggacaa agtgttggct ggatccctct atactgatac aaatgaacag
      601 ggattaacga ttccaagcag tttactaaaa aattggaatg aacagacagg aaaaaatttg
      661 acagctaatg atcttattgg caaatcagtc tcagccagca ttgtagaaag tgctgccgaa
      721 gctagtaaga ttgctcaatt tcaaacgaag attgtacgtg taatcaatga tgaagatgac
      781 atggaggaca gcaacagttt catgctgtct catcaaatgg aaacgatttt gaaagaggct
      841 ggatttacga aagctgtatc ttattttatc ttggaactca aagatccatc acagacaaaa
      901 gtagtaacag aagaattaca gaaaaataag aagtatactg tgctttctca acagagagtt
      961 cttgatattg tgattacctt tattcgtgtt attcagggat tattgattgt gctttcatca
     1021 caagctattg tggtagcagc ggttatgatt ggtatcatta tttacatcaa tatcatgcaa
     1081 cgttccaagg aaataggtgt catgaaagca gttggttatc agaatcgtga tgtcaaagga
     1141 atttttattt acgaggctat ctggattgta ggtatcgcct tgctgctggc atttttggtt
     1201 gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc acttttaccc atccatcact
     1261 aaggtttttg aattaaatct tttatctgtt ttaggaactc tagttttcgc tctattactt
     1321 ggttatgtct cagcctactt cccggcgcgt aagattagta aaatggatcc tatagaatcg
     1381 ttatgctatg aatagtaagg gattccttgg tttacagtct taaaaagaaa ctaacataga
     1441 aaaacaatgg ccatacttgc aattcaactc aaatagttgt ataataggtt tgttgaaagg
     1501 tgatttgtag tgattcaagt tactcttttc acaataaaaa tttcatgatt ttcataagga
     1561 ggaaatcact aatggtagtt aaagttggta ttaacggttt cggacgtatc ggtcgtcttg
     1621 ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac acgcatcaac gaccttacag
     1681 atccagttat gcttgcacac ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta
     1741 ctgttgaagt taaagaaggt ggatttgaag ttaacggtaa attcatcaaa gtttctgctg
     1801 aacgtgatcc agaacaaatc gactgggcta ctgacggtgt agaaatcgtt cttgaagcta
     1861 ctggtttctt tgctaagaaa gaagcagctg aaaaacacct taaaggtgga gctaaaaaag
     1921 ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt tgtattcaac actaaccacg
     1981 acgttcttga cggtactgaa acagttatct caggtgcttc atgtactaca aactgcttgg
     2041 ctccaatggc taaagctctt caagacaact ttggtgttgt tgaaggattg atgactacta
     2101 tccacgctta cactggtgac caaatgatcc ttgacggacc acaccgtggt ggtgaccttc
     2161 gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc aactggtgct gcaaaagcta
     2221 tcggtcttgt aatcccagaa ttgaatggta aacttgacgg atctgcacaa cgcgttccaa
     2281 ctccaactgg atcagttact gaattggtag cagttcttga aaagaacgtt actgttgatg
     2341 aagtgaacgc agctatgaaa gcagcttcaa acgaatcata cggttacaca gaagatccaa
     2401 tcgtatcttc agatatcgta ggtatgtctt acggttcatt gtttgacgca actcaaacta
     2461 aagttcttga cgttgacggt aaacaattgg ttaaagttgt atcatggtac gacaacgaaa
     2521 tgtcatacac tgcacaactt gttcgtactc ttgaatactt cgcaaaaatt gctaaataat
     2581 tcttgagttg atagaaagca aggctttgtg gtcttgcttt ttatatggaa aaatggatga
     2641 cacgatcatc cattcttttt taattctttt tcaaatgtat ttgaaagggt agtgaaagtt
     2701 agcttctcta aagtaagtgg gtgggtaaag gaaagtcgga aggcatgaag cataagccga
     2761 cttgtctttg atttactatt atagagaggg tcgcccagga taggaagatt atgatgcgaa
     2821 aggtgcacac gaatctgatg ggttcgccct gtctttagct tgcaacgagc caaggaagtc
     2881 ttgtttgaga attgctttaa tctgcttaca tgcgtttcag catattgccc attttttgca
     2941 tcaactattc tttttctacg atcatggcga tcacgtccaa ttttgtctct gaaaacaagt
     3001 tcttttctgt tgatatttcc atcaactaga gcccaatatt ctctagaaat ctcttttttc
     3061 tccaataagc gattgagaat gggcaggata aaaggatttt tggcaaagag aactaagcca
     3121 ctggtttcca tgtccagacg atgaacgaca tagcaggttt ggccaacata ggtactgaca
     3181 tggttaagaa gggcaatttc gtttggttga ttaccatgcg ttttcatccc ctctggtttg
     3241 tttacaataa tcaagtgttg atcttgataa acttcctgca ctaagtctgg gttgccccaa
     3301 gggatcgtct tttggggata atcttcctcg tcaaaagtca actggcaaac atctccagga
     3361 tttacgattt cgttccagtg gacttcttct tgatttatca aaatatgttt cttgattctc
     3421 aaaaaatgac ggatttttct agggatgagg agttgttcct caagtaattg ctttaccgtc
     3481 atttgaggta gagaggcggg taatgtaaat gtgaattgca tacagatatt gtaacaaaaa
     3541 aagccctatt tggataggaa atagctaaat tcttgtcttc ctatgatgaa gatgataaaa
     3601 taaacgcatg aaattagata aattatttga gaaatttctt tctcttttta aaaaagaaac
     3661 aagtgaacta gaggactctg attctactat cttacgtcgc tctcgtagtg atcgaaaaaa
     3721 attagcccaa gtaggtccga ttcgaaaatt ctggcgtcgt tatcatctaa caaagattat
     3781 ccttatacta ggtttgagtg caggattgct agttggaatc tatttgtttg ctgtagccaa
     3841 gtcgaccaat gtcaatgatt tgcaaaatgc cttgaaaact cggactctta tttttgaccg
     3901 tgaagaaaaa gaggctggtg ccttgtctgg tcaaaaggga acctatgttg agctgactga
     3961 tatcagtaaa aacttgcaga atgctgttat tgcgacagaa gaccgttctt tctataaaaa
     4021 tgacgggatt aactatggcc gtttcttctt ggctattgtc actgctggac gttcaggtgg
     4081 tggctctacc attacccaac agctggctaa aaacgcctat ttatcacaag atcaaactgt
     4141 tgagagaaaa gcgaaagaat ttttccttgc cttagaatta agcaaaaaat atagtaagga
     4201 gcaaattcta accatgtacc ttaacaacgc ttattttgga aatggtgtgt ggggtgtaga
     4261 agatgcgagt aagaaatact ttggagtttc tgcatcagaa gtgagtctgg atcaagctgc
     4321 gactctggca gggatgctca aggggccgga actgtataat cccttgaatt ccgtagaaga
     4381 ttctactaat cggcgcgata ctgtcttgca gaatatggtt gcagcaggat atattgataa
     4441 aaaccaagaa accgaagctg ctgaagttga tatgacttcg caattgcacg ataagtatga
     4501 aggaaaaatc tcagattacc gttacccctc ttattttgat gcggtggtta atgaagctgt
     4561 ttccaagtat aatctaacag aggaagagat tgtcaataat ggctaccgca tttacacaga
     4621 gctggaccaa aactaccaag caaatatgca gattgtttat gaaaacacat cgctatttcc
     4681 gagggcagag gatggaacgt ctgctcaatc aggaagtgta gctctcgaac cgaaaacagg
     4741 gggagttcgt ggagttgtcg gtcaagttgc tgacaatgat aaaactggat tccggaattt
     4801 caactatgca acccaatcaa agcgtagtcc tggttctaca attaagcctt tagttgttta
     4861 tacgccagca gttgaagcag gctgggcttt gaataagcag ttggataacc ataccatgca
     4921 gtatgacagc tataaggttg ataactatgc agggatcaaa acgagtcgag aagttcctat
     4981 gtatcaagcc ttggcagaat cgcttaatct acctgctgtt gccactgtta atgatttggg
     5041 tgtcgacaag gcttttgagg caggcgaaaa attcggactc aacatggaaa aggtcgaccg
     5101 tgttcttggt gtcgccttgg gaagcggtgt tgaaaccaac cctcttcaaa tggctcaagc
     5161 atacgctgcc tttgcaaatg aaggtttaat gcctgaagct cattttatta gtagaattga
     5221 aaatgctagt ggacaagtta ttgcgaatca taaaaattca caaaaacggg tgattgataa
     5281 gtctgtagct gacaagatga ccagtatgat gttggggact ttcaccaacg gtaccggtat
     5341 tagttcatcg cctgcagact atgtcatggc agggaaaact ggaacaactg aagcagtttt
     5401 caatccggag tacacaagtg accagtgggt aattggttat actccggatg tagtgattag
     5461 ccactggctt ggctttccga ccactgatga aaatcactat ctagctggct ctacttcaaa
     5521 cggtgcagct catgtcttta gaaacattgc caataccatt ttaccttata cgccaggaag
     5581 tacctttacg gttgaaaatg cttataagca aaatggaatt gcaccagcca atacaaaaag
     5641 acaagtacaa accaatgata atagccagac agatgataat ttgtctgata ttcgagggcg
     5701 tgcgcaaagt ctagtagatg aggctagccg ggctatctca gatgcgaaga ttaaggaaaa
     5761 ggctcaaaca atatgggatt cgatagtcaa tctatttcgc taagatgctt gtcaaagcct
     5821 agctttcttg ttataatgga taagatggag gcgttatggc actaaaaaaa gcaagcctag
     5881 cttgtgcggt ttgtggttcg agaaactatt caatcaagat cagcggaaac cccaagccta
     5941 cacgactaga agtaaataaa ttttgtaagc attgtggcaa gtacactaca cacagagaaa
     6001 cgagatagga gagagcgatg cgttttattg gagatatttt tagacttctt aaagacacaa
     6061 cgtggccaac tcgcaaggaa agctggagag attttcgttc tatcatggaa tacacagctt
     6121 tctttgtagt aattatttac atttttgacc agttgattgt ttcaggtttg attcgattta
     6181 ttaacatttt ttagaagatt agtggagtta attacactag aaatcttcta tttatgaaag
     6241 gaaatatcat ggatagtttt gataaaggat ggtttgtttt acaaacttat tctggttatg
     6301 aaaataaggt gaaagaaaat ctattacaac gtgcacaaac ctacaatatg ttggataata
     6361 ttctacgcgt tgaaattcca acacaaacag tgcaagttga aaaaaatgga aagagaaaag
     6421 aagtagaaga aaatcgcttt ccaggttatg ttcttgtaga aatggtcatg acagatgaag
     6481 cttggtttgt tgttcgaaac acaccaaatg ttacaggatt tgtcggatca cacgggaaca
     6541 gatcaaaacc aactccatta ttggaacaag aaattcgcga cattttggta tctatgggac
     6601 aaactgttca agaatttgat ttcgatgttg agattggtca aactgtacgt attattgatg
     6661 gtgcttttgc agactacact ggtaagatta cagaaattga taataacaaa gtgaaaatga
     6721 ttatctctat gtttggtaat gacacagttg cagaagtaaa tctaaaccaa atcgcagaat
     6781 tataaccctg agagaggctg gagcctctct tttttgtgca gtttag
//
LOCUS       DAVKZB010000093         6768 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKZB010000093 DAVKZB010000000
VERSION     DAVKZB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.093"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            70..1329
                     /locus_tag="VXA80_001619"
     CDS             70..1329
                     /locus_tag="VXA80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV0354085.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1405..1540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1579..2079
                     /locus_tag="VXA80_001620"
     CDS             1579..2079
                     /locus_tag="VXA80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV0354086.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2155..2523
                     /gene="rplL"
                     /locus_tag="VXA80_001621"
     CDS             2155..2523
                     /gene="rplL"
                     /locus_tag="VXA80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV0354087.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2722..2895
                     /locus_tag="VXA80_001622"
     CDS             2722..2895
                     /locus_tag="VXA80_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354088.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
     gene            2892..3140
                     /locus_tag="VXA80_001623"
                     /pseudo
     CDS             2892..3140
                     /locus_tag="VXA80_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3154..3477
                     /locus_tag="VXA80_001624"
     CDS             3154..3477
                     /locus_tag="VXA80_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354089.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGADAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKQPINFANLLDSLIVSEYQMEPNIW
                     EVSHD"
     gene            3470..3850
                     /locus_tag="VXA80_001625"
     CDS             3470..3850
                     /locus_tag="VXA80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0354090.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLYN"
     gene            3863..4006
                     /locus_tag="VXA80_001626"
     CDS             3863..4006
                     /locus_tag="VXA80_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354091.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            4099..4686
                     /locus_tag="VXA80_001627"
     CDS             4099..4686
                     /locus_tag="VXA80_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0354092.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRNLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4888..5025
                     /locus_tag="VXA80_001628"
                     /pseudo
     CDS             4888..5025
                     /locus_tag="VXA80_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            5315..6718
                     /locus_tag="VXA80_001629"
     CDS             5315..6718
                     /locus_tag="VXA80_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV0354093.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT         2207 a         1067 c         1422 g         2072 t
ORIGIN      
        1 acctacggca aagtgaatct gaagtggttt gaagagagta caacttgtct tttagaaaag
       61 gagcctataa tgaaagtctt tcagcatgta aatatcgtga cttgtgatca agatttccat
      121 gtttatcttg atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat
      181 aagccagcgt ttttagagca agctgagcag attatagact atcagggagc ttggattatg
      241 cctggtttgg tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat
      301 gacagcaatc tccatgaatg gctcaatgac tatatctggc cagcagaatc tgagtttact
      361 cccgacatga ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca
      421 acaaccttta acgatatgta taatcccaat ggtgtggata ttcagcaaat ttatcaggtg
      481 gtgaaaactt ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa
      541 acaactgctg agactataag cagaactcga tccatcatag acgaaatctt aaaatataaa
      601 aatccaaatt tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg
      661 ctggaagcga gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg
      721 gagaccaagg aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt
      781 cttgaagaac tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac
      841 gagagagaaa ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt
      901 aacctcaaac tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca
      961 gtcggaattg cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga
     1021 aggactgcag ctcttcttca gaagatgaaa agtggggatg ccagccagtt tccaatcgaa
     1081 acagctctca aggtactgac aatcgaaggg gctaaggccc ttggaatgga aaatcagata
     1141 ggaagtctgg aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt
     1201 catctccaac ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat
     1261 gtagatgatg tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta
     1321 gaactttaaa agaaaaatca cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct
     1381 ttttttgact atgctatact tatatacggt ttaaaaaaac tgcctaagac agtaggggag
     1441 ctcgactcat aaatatccta ccgaggacaa gacgtatcat gtaaaaagaa gcgtattgta
     1501 ctttcgtgtc taggtttggg cgcgtttttc tttttgaaaa attccccaag caaaataatt
     1561 acggaggtga acacactaat gagtgaagca attattgcta aaaaagcgga actagttgac
     1621 gtagtagctg aaaaaatgaa agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg
     1681 acagttgagc aagatacagt tcttcgtcgc gagcttcgtg gaagcgaagt tgagtataaa
     1741 gttattaaaa actcaatctt gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca
     1801 tctgtatttg ttggaccatc tgcagtagca ttttctaatg aagatgttat cgcaccagcg
     1861 aaaatcttga acgacttttc taaaaacgct gaagcacttg aaattaaagg tggtgcaatc
     1921 gaaggcgctg tcgcatctaa agaagagatt cttgcacttg caactcttcc aaaccgcgaa
     1981 ggacttcttt ctatgctcct ttctgtactt caagcgccag tgcgcaacgt tgctcttgca
     2041 gtcaaagcgg ttgcagaaag caaagaagac gcggcttaat cttaagctac acagcgtagc
     2101 ctagctacga aaaaaactat tataaaattt aaaacttatt tggaggaaat aacaatggca
     2161 ttgaacattg aaaacattat tgctgaaatt aaagaagctt caatccttga attgaacgac
     2221 cttgtaaaag ctatcgaaga agaatttggt gtaactgcag ctgctcctgt agctgttgct
     2281 gcagctgatg cagctgatgc tggtgctgct aaagattcat tcgacgttga attgacatct
     2341 gcaggcgaca aaaaagttgg tgttatcaaa gttgtacgtg aaatcactgg tcttggtctt
     2401 aaagaagcta aagaacttgt tgacggtgca ccagcacttg ttaaagaagg cgttgcaact
     2461 gcagaagctg aagaaatcaa agctaaattg gaagaagctg gagcttcagt tactcttaaa
     2521 taagaggcat ataccaatta gaattcggaa tactatagta tcagcctttt agaatcgtga
     2581 acacatttta gaaactgata aatttattta gtttcctacc aaaaacccta ccaaaaattc
     2641 tttggcaggg tttttgtttt aataaacttt cgggggtaga cactagattg tctacccttt
     2701 ttttgtgttt cggaggtaat tatgcaaaag gaaggacagt catcacgtca attcgtgaag
     2761 agtcatctca tgactatcat gggagttgat attgtaaaag caactcagtt agttgatgaa
     2821 atggaacaag taggtttaat ccgatttgat gaatttggaa atgttggaat tttagtcttg
     2881 gagggacaat catgaagcgt attaccgcta gtcaatatca gacatcagag cgttattata
     2941 agctccccaa acttttattt gagagtgaac ggtataagaa tatgaaactg gaggttaagg
     3001 tggtttactc agttttaaaa gatcggttgg agttgtcttt gagcaaaggt tggattgatg
     3061 aggatggggc tatttatttg atttattcca attcaaattt gatggcactt ttaggctgtt
     3121 caaagtcaaa attactctcc atgtgagttt gaagtgacat ttttagatga ttaccataaa
     3181 aaacacaact accccctatt ttacgaatcc taccttcaaa acgttatgga attccttgaa
     3241 agtcaggata taaagaacgg agctgatgcc tttgtagatg atcatcaaaa tcttgttttt
     3301 gttttatatg gacaaggcta tcgagccgag ggaaaagagg gaatacttac aacccaagta
     3361 actgtaaaag cttatgatga agacaagcaa ccgattaact tcgcaaattt attagattcc
     3421 ttaatcgtat cagaatatca aatggaaccg aatatttggg aggtctccca tgattgatct
     3481 ctatctaagt aaaaatagcc aaagaaatca acttctttta gactttttcc aaaactatgg
     3541 tatcgaggta tcttgtcatt cagtttctga aatgacaagg gataaattaa ttgagatgat
     3601 gagttattct tcagattgtt ttgagttttt atctcccaat ttattgcgat ttaagaaccg
     3661 tgacaatcta agattaacgg atttcgttga gataatttta aaaaatcctg aactaaccat
     3721 cagacttcct cttgtggttt caaataagcg ggtttatcca agtctgaatt tggaagaggc
     3781 tagagctcta ttgccaagag atacgaaaca attgatttac atggcacaaa cacattattt
     3841 atataactaa aggagaagca atatggctga acgattttgg gagaacttgt ccattatttt
     3901 ggctgaaaga aatatcagct ggattgagtt aaccagaaaa atgtttgcgg gagagtttca
     3961 ctatccaagt gaattgaatc gtttgtatca aaagattcgc cactagaaga tggaacaatg
     4021 aatgcctcaa agtccatggg tagaaaggat tgtgcaggta ttagatttag attatgaaga
     4081 tttatttcgg aggtaactat gaaaagaata attccagttt atatattcca acaagtaaat
     4141 gtcctattgg tatctctata cttattgaaa tttctttgta tcggtgagtt aactatacta
     4201 cagattctct atggttcgtc actcatttct tttttatgga tgtatggcca acgcaaacaa
     4261 gcgcataagg tcaatatgaa atctaggatg aaatggcttg gtgttgaatt tgttagccta
     4321 ctgattataa gtctatgttt tagtctgatt catgctcaag gaagcacgaa tcaagcaaac
     4381 ttaattggcc ttcaacatca aattccttgg ttttcatttt tattgttctt aatcaatgcg
     4441 agtatggttg aagaatttct gtatcgagaa attttatgga atttggtcag aaatttagat
     4501 attcgagttg ctttgacaag tgttttattt gccttagcac atcatacagg aaccattcta
     4561 gcttggtgtt tgtatgtttc acttgggatg tttttaggta tggtgcgcta taaatctgac
     4621 ttatggggca gtatgggact acatttggtg tggaacctac tagtttatag tttgctgctt
     4681 ttttaaaata ggtaacctga tttttaggta acttctaaaa ctaacttcca gttttgggag
     4741 agagatggaa gttactttga aaagttacgg aaacattatt ttctatgtga caacattgta
     4801 tcattgcttg cattttactg ctggagagag tataatgaag gtggataaag cacttaatcc
     4861 taaaaattta aataggagga gttagctatg gcagaagaag tattaaatct ttaattagta
     4921 tctgttcaag ttgacgaaac agatgaagtt gatggaatgc gatttagtac attcagtaca
     4981 aaccgttgcg gtaactggag tgcttttagt tgggaaaatt gttaattcat ttaaattgta
     5041 aactttagag attctctgga gttatggatc tcgttccaat atgtgcacgt tggaatgtca
     5101 gtgcttatat ggggggaatc caaaggattc tcccttttta ttttaataag tctgtctagc
     5161 tatagatata ttaagtttga tagaatttct tggagatttt gttatggatt ataatttcaa
     5221 tttggagcat cctttctttt tcactaataa tgattatagt acagatacta gcataaagta
     5281 ctaagtaagt ctgcctttta attggcacga agtaatgaat aatgatgagt gggtttatca
     5341 ataccctatt ggaaaatttg ttgaaagaca agggtggaaa atacacatta gttcagagta
     5401 taatagttca cacgagttgt tacaagatgt agcaaaaatt tgccatgaaa tgcgaattcc
     5461 atttaaacat cttagtacag aagacaaatt tatcatgcga aacgggaaat tggttagtcg
     5521 aggattttct ggtaaattta ttacatgtta tccaaatcaa aatgaattag agtcggtttt
     5581 gcaaaggtta gaaagtgcct taaagcaata taatgggcca tatattttaa gcgataaacg
     5641 ttgggatgaa gcaccaatat atctgaggta tggtgtgttt aggccgtcaa gggatgatga
     5701 aaagaaggtt gttattgatg aattaattgt tggcgatgaa gttgttaaag atgaacgttt
     5761 accagtattt aaaattccaa aaggtatagt gccccctgac tttttgaata aatggcttga
     5821 caagaaagat aaaaaacagg gagactttcc ctttattata gataatgcaa ttagattttc
     5881 aaacagtggt gggatatata atgctagact aaaagaagat gggaaaaaaa taattttaaa
     5941 agaggctaga ccgtatacag gtctaggatt tgatggtact tattcttcag agagattagc
     6001 ttcggagtgc aaagcactaa aaattctaaa tgaatggagt gagatgccta aaatatactg
     6061 gtacggtaag atctgggagc acaccttctt aggaattgaa catatgaagg gcgttccact
     6121 aaatagatgg gttaccaata attttccttt atatgaagtt gttgataaga ccaaagatta
     6181 tctattgaga gtttcaaaga tagtagaaaa actcatcgat ttaacgaata agtttcattc
     6241 tgaaaatgta tatcatcaag atttacattt aggaaatatc ttggttaagg atgaagatga
     6301 gattagtata atagactggg aacaggctgt cttttcgaat gatgaaaaag ttgtacataa
     6361 agttgcagcc cctggtttta gagcatggcg agaaacactt cccagtgaga tagattggta
     6421 tggtattaga caaatagctc attatttata catgccacta gtaactacat cggatttgac
     6481 atacaactat gtaagtcaaa ccaggataga aggaaaaaaa ctatttgagt ctttgggtta
     6541 tacaagagaa catatagatt atgtagaaag tttattgtca tatctagatt ctaaatgtcc
     6601 acagattgag aatatttcca ggaaaaaagt tttaaaaccc atgcatgaga ttagaactat
     6661 cgagagtgag caagatatac aagattttat tataaaagct tttcttttag tgaactgata
     6721 ggtattttct tagcaaacga taaagtttta gggccgatac aatcaatt
//
LOCUS       DAVKZB010000094         6729 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKZB010000094 DAVKZB010000000
VERSION     DAVKZB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.094"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..157)
                     /locus_tag="VXA80_001630"
     CDS             complement(<1..157)
                     /locus_tag="VXA80_001630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354094.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     SKHCFEVV"
     gene            complement(211..507)
                     /locus_tag="VXA80_001631"
     CDS             complement(211..507)
                     /locus_tag="VXA80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0354095.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPDAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            729..1493
                     /locus_tag="VXA80_001632"
     CDS             729..1493
                     /locus_tag="VXA80_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV0354096.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            2058..3437
                     /locus_tag="VXA80_001633"
     CDS             2058..3437
                     /locus_tag="VXA80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0354097.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3451..4116
                     /locus_tag="VXA80_001634"
     CDS             3451..4116
                     /locus_tag="VXA80_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV0354098.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4251..4409
                     /locus_tag="VXA80_001635"
     CDS             4251..4409
                     /locus_tag="VXA80_001635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354099.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            4471..5010
                     /locus_tag="VXA80_001636"
     CDS             4471..5010
                     /locus_tag="VXA80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0354100.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYKENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            5077..5487
                     /locus_tag="VXA80_001637"
     CDS             5077..5487
                     /locus_tag="VXA80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV0354101.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5603..>6729
                     /gene="pavB"
                     /locus_tag="VXA80_001638"
     CDS             5603..>6729
                     /gene="pavB"
                     /locus_tag="VXA80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV0354102.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG"
BASE COUNT         1836 a         1308 c         1544 g         2041 t
ORIGIN      
        1 ctacaacctc aaaacagtgt tttgagctga cttcgtcagt tctatctaca acctcaaaac
       61 agtgttttga gctgacttcg tcagttccat ccacaacctt aaaacagtgt tttgagcagc
      121 ctgcggctaa cttcctagtt tgcactttaa ttttcattga gtattacttc atcttctcct
      181 gaaattgagt ttttgcccag ctcctcttga ttattctact tgaccgggcc aagcattcat
      241 accaccttct acgttggtaa cggtgaggcc ttgggagctg agaaattggc aggtagatgc
      301 agagcggact cctccttgac aaataacata gtattcatgg tcaggtttga gttctttgta
      361 gccttgctct aaggtactta atggaagatt tttggcatct ggtgcatgtc ctgcttggaa
      421 ttcatgtgct tcacggacat cgataagttc tagattttca ttttgatatt ttccataaaa
      481 gtcagccatg ctgatactag tttccatatt ctactccttc tgggttagtc attttgtata
      541 gtgaataagc gccgtcaagg ttttggacgg taaatcctgc ttgcttgagg ataggctctg
      601 cgatatagct gcgcaaacca ctgaggcagc tgacgatgta ggcttgtttt tctcctcagt
      661 tcatcagctt cctccttgac actttgtcag attttgatac aatagtacaa aattagagga
      721 ggcaggctat gattcagaaa catgcgattc ctattttaga gtttgatgac aatcctcagg
      781 cggttatcat gcccaatcac gaggggctgg acttgcagtt gccaaagaag tgtgtttatg
      841 catttttagg tgaggagatt gaccgctatg caagggaagt aggggcggat tgtgtcggcg
      901 aattcgtttc tgctaccaag acctatccag tctatgtcat caactacaag ggtgaggagg
      961 tctgtctggc tcaggctcct gttggctccg ctccagcagc ccagtttatg gattggttga
     1021 ttggctatgg tgtggagcag attatctcta ctgggacctg tggtgtgcta gccgatatag
     1081 aggaaaatgc ctttctagtc cctgttcgcg ctctgcgaga tgagggagcc agttaccact
     1141 atgtggcacc ttgtcgttat atggaaatgc agccagaggc tattgctgct attgaggaag
     1201 ttttggaaga cagagggatt ccttatgaag aagtcatgac ctggacgaca gacggttttt
     1261 accgagaaac ggctgaaaag gtggcttatc gtaaggaaga aggctgtgct gttgtggaga
     1321 tggagtgttc tgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt
     1381 tgttcacagc agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg
     1441 aagcttttaa taaggcgcta gaactgagtt tagcaagtgt tcaccacctt tagttgtaca
     1501 ggcaaaggat ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa
     1561 cgaggtcacc ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga
     1621 aaaacgtatc atatcaggtg atgaaaattt tgatactatg cgttttatgt tggtaagatt
     1681 tagagtgaga tgaaatgata ctcttcgaaa atctcttcaa accaggtcag cttcaccttg
     1741 ccgtatagat gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
     1801 aacctgtgac tagctttcta agcgatgcct tggttctcat tgcctataat caaaaagaga
     1861 aattttctcc tgaaaagcat atagagtagc tggcgttaaa agctcctgtc ttgctttttt
     1921 gacctatagt cacatctatc aagtattgtt cttgcctaag ctatcaataa aaaggtggca
     1981 ttttttaggc ttggtgttag tgaattttgc cttatcctat ctaagtcatt tcgagctttt
     2041 tatggtacaa tggaaacatg ttattcaaat tatctaagga aaaaatagag ctaggcttat
     2101 ctcgtttatc gccagcccgt cgtatttttt tgagttttgc cttggtcatt ttactaggct
     2161 ctcttctttt gagcttgccc tttgtccaag ttgaaagctc acgagcgact tattttgatc
     2221 atcttttcac tgctgtctct gcagtctgtg tgacaggtct ctcaaccctt ccagtagctc
     2281 acacctataa tatctggggc caaataatct gtttgctctt gattcagatc ggtggtctag
     2341 ggctcatgac ttttattggg gttttctata tccagagcaa gcaaaagctt agtcttcgta
     2401 gccgtgcaac tattcaggat agttttagtt atggagaaac tcgatctttg agaaagtttg
     2461 tctattctat ttttctcacg acctttttgg ttgagagctt gggagctatt ttgcttagtt
     2521 ttcgccttat tcctcaactt ggctggggac gtggtctttt tagttccatt tttctagcga
     2581 tctcagcctt ctgtaatgcc ggttttgata atttagggag caccagttta tttgcttttc
     2641 agaccgattt actggtcaat ctggtgattg caggcttgat tattacaggc ggccttggtt
     2701 ttatggtctg gtttgatttg gctggtcatg taggaagaaa gaaaaaagga cgtctgcact
     2761 ttcatacgaa gcttgtacta ttattgacta taggtttgtt gttatttgga acagcaacta
     2821 ctctctttct tgagtggaac aatgctggaa cgattggcaa tctccctgtt gccgataagg
     2881 ttttagttag cttttttcaa acagtgacga tgcgaacagc tggcttttct acgatagatt
     2941 atactcaggc tcatcctgtg actcttttga tttatatctt acagatgttt ctaggtgggg
     3001 cacctggagg aacagctggg ggactcaaga ttacgacatt ttttgtcctc ttggtctttg
     3061 cacgaagtga gcttctaggc ttgcctcatg ccaatgttgc gagacgaacg atcgcgccgc
     3121 gaacggttca aaaatccttt agtgtcttta ttatcttttt gatgagcttc ttgataggat
     3181 tgattctgct agggataaca gccaaaggca atcctccctt tatccacctc gtatttgaaa
     3241 ccatttcagc tcttagtaca gttggtgtaa cggcaaatct gactcctgac cttgggaaat
     3301 tggctctcag tgttatcatg ccacttatgt ttatgggacg aattggtccc ttgaccttgt
     3361 ttgttagctt ggcagattac catccagaaa agaaagatat gattcactat atgaaagcag
     3421 atattagtat tggttaagaa aggaaagagc atgtcagatc gtacgattgg aattttgggc
     3481 ttgggaattt ttgggagcag tgtcctagct gccctagcca agcaggatat gaatattatc
     3541 gctattgatg accacgcaga gcgcatcaat cagtttgagc cagttttggc gcgtggagtg
     3601 attggtgaca tcacagatga agaattattg agatcagcag ggattgatac ctgcgatacc
     3661 gttgtagtcg cgacaggtga aaatctggag tcgagtgtgc ttgcggttat gcactgtaag
     3721 agtttggggg taccgactgt tattgctaag gtcaaaagtc agaccgctaa gaaagtgcta
     3781 gaaaagattg gagctgactc ggttatctcg ccagagtatg aaatggggca gtctctagca
     3841 cagaccattc ttttccataa tagtgttgat gtctttcagt tggataaaaa tgtgtctatc
     3901 gtggagatga aaattcctca gtcttgggca ggtcaaagtc tgagtaaatt agacctccgt
     3961 ggcaaataca atctgaatat tttgggtttc cgagagcagg aaaattcccc attggatgtt
     4021 gaatttggac cagatgacct cttgaaagca gatacctata ttttggcagt catcaacaac
     4081 cagtatttgg ataccctagt agcattgaat tcgtaaagag ggatgacccc tcttttttga
     4141 tgcctaagat ggcaaataga gacagaagcc ccttgtcttc tagtaaaagt tcttcaaagg
     4201 ctggacttta tggtaaaata gaaagaagtg acaagagaga gtaatactca atgaaaatca
     4261 aagatcaaac taggaaacta gctacgggct gctcaaaaca ctgttttgag gttgtggata
     4321 gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact gacgaagtca
     4381 gtaacatcta tacggcaagg cgacgttgac gcggtttgaa gagattttcg aagagtataa
     4441 gaaaaaatca gtcccctaaa ggagtagatt atgaagttat tgtctatcgc aatttctagc
     4501 tataatgcag cagcctatct tcattactgt gtggagtcgc tagtgattgg tggtgagcaa
     4561 gttgggattt tgattatcaa tgacgggtct caggatcaga ctcaggaaat cgctgagtgt
     4621 ttagctagca agtatcctaa tatcgttaga gccatctata aggaaaataa atgccatggc
     4681 ggtgcggtca atcgtggctt ggcagaggct tctgggcgct attttaaagt agttgacagt
     4741 gatgactggg tggatcctcg tgcctacttg aaaattcttg aaaccttgca ggaacttgag
     4801 agcaaaggtc aagaggtgga tgtctttgtg accaattttg tctatgaaaa ggaagggcag
     4861 tctcgtaaga agagtatgag ttacgattca gtcttgcctg ttcggcagat ttttggctgg
     4921 gaccaggtcg gaaatttctc caaaggccag tataccatga tgcactcgct gatttatcgg
     4981 acagatttgt tgcgtgctag ccagttctaa ctgcctgaac atacttttta tgtcgataat
     5041 ctctttgtct ttacgcccct tcagcaggtc aagaccatgt actatctgcc tgtcgatttc
     5101 tatcgttatt tgattgggcg tgaggaccag tctgtcaatg agcaagtgat gattaagtgc
     5161 attgaccagc aactcaaggt caatcgactc ttgatagacc aacttgattt gtcccaagtg
     5221 agtcatccca aaatgcgaga atatctgctg aatcatattg aactcacgac ggtgatttcc
     5281 agtaccctgc tcaaccgatc tggaacagcg gagcatctgg caaaaaaacg ccaattgtgg
     5341 acctatattc agcagaaaaa tccagaagtc tttcaggcta ttcgtaagac catgttgagc
     5401 cgtttgacca aacattctgt cttgccagat cgcaaactgt ccaatgtcgt ctatcaaatc
     5461 accaaatctg tttatggatt taattaatat aagtgtttta taagagggat ttaagaaaaa
     5521 ttttaacttt ttcttagtcc tttttaattt caggagatta tactagagtc atcaaataaa
     5581 gaaagactct aaggagaatc ctatgaaatt caatccaaat caaagatata ctcgttggtc
     5641 tattcgccgt ctcagtgtcg gtgttgcctc agttgttgtg gctagtggct tctttgtcct
     5701 agttggtcag ccaagttctg tacgtgccga tgggctcaat ccaaccccag gtcaagtctt
     5761 acctgaagag acatcgggaa cgaaagaggg tgacttatca gaaaaaccag gagacaccgt
     5821 tctcactcaa gcgaaacctg agggcgttac tggaaatacg aattcacttc cgacacctac
     5881 agaaagaact gaagtgagcg aggaaacaag cccttctagt ctggatacac tttttgaaaa
     5941 agatgaagaa gctcaaaaaa atccagagct aacagatgtc ttaaaagaaa ctgtagatac
     6001 agctgatgtg gatgggacac aagcaagtcc agcagaaact actcctgaac aagtaaaagg
     6061 tggagtgaaa gaaaatacaa aagacagcat cgatgttcct gctgcttatc ttgaaaaagc
     6121 tgaagggaaa ggtcctttca ctgccggtgt aaaccaagta attccttatg aactattcgc
     6181 tggtgatggt atgttaactc gtctattact aaaagcttcg gataatgctc cttggtctga
     6241 caatggtact gctaaaaatc ctgctttacc tcctcttgaa ggattaacaa aagggaaata
     6301 cttctatgaa gtagacttaa atggcaatac tgttggtaaa caaggtcaag ctttaattga
     6361 tcaacttcgc gctaatggta ctcaaactta taaagctact gttaaagttt acggaaataa
     6421 agacggtaaa gctgacttga ctaatctagt tgctactaaa aatgtagaca tcaacatcaa
     6481 tggattagtt gctaaagaaa cagttcaaaa agccgttgca gacaacgtta aagacagtat
     6541 cgatgttcca gcagcctacc tagaaaaagc caagggtgaa ggtccattca cagcaggtgt
     6601 caaccatgtg attccatacg aactcttcgc aggtgatggc atgttgactc gtctcttgct
     6661 caaggcatct gacaaggcac catggtcaga taacggcgac gctaaaaacc cagccctatc
     6721 tccactagg
//
LOCUS       DAVKZB010000095         6529 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKZB010000095 DAVKZB010000000
VERSION     DAVKZB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.095"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..1437
                     /locus_tag="VXA80_001639"
     CDS             <1..1437
                     /locus_tag="VXA80_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0354103.1"
                     /translation="GVNNWGLSTDIKWLLLSALLSFLGGGVVLFSQGLFYPILLATYG
                     FGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGRLPLKRLMILTLLCQLVAVFTV
                     GYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGLGDRGKDTENQSKWYAFAKEAI
                     EEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANTLYVSPSYLDKEKVVVDAETKQ
                     KLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYGKSGEEASAPLDYEMKAHVSYL
                     SMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDSFISLSSWSINAGKQLFIKGYE
                     SGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETATLILGAIVGIASSLLLFYSVN
                     LLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFVFGASLFILSSRDLVIGLLTLL
                     VFLVSAVLTLYRQAQKESRVSMTIMKGK"
     gene            1439..2080
                     /locus_tag="VXA80_001640"
     CDS             1439..2080
                     /locus_tag="VXA80_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354104.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            2184..3536
                     /locus_tag="VXA80_001641"
     CDS             2184..3536
                     /locus_tag="VXA80_001641"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV0354105.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            3540..4598
                     /locus_tag="VXA80_001642"
     CDS             3540..4598
                     /locus_tag="VXA80_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV0354106.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            4608..5807
                     /gene="divIB"
                     /locus_tag="VXA80_001643"
     CDS             4608..5807
                     /gene="divIB"
                     /locus_tag="VXA80_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV0354107.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     DKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT         2013 a         1148 c         1446 g         1922 t
ORIGIN      
        1 ggggtgaata attggggttt atctactgat atcaagtggc tccttctatc agccctcctt
       61 tccttcctag gtgggggagt cgttcttttt agtcaaggtt tgttttatcc tatcttgtta
      121 gccacctatg gttttgggat tagtttctat ctgttgtttt tattggcgat ttcaatttta
      181 ctaatgcttc tttatctaat gagtttgaat tacaaagcat tagttcccgt tattaagggg
      241 agattacccc ttaaacgcct gatgatttta accctattgt gtcagttagt agctgttttt
      301 acagtaggat acgctgttaa gacaggtttg acgtcttacc aacgattgaa agaacttgaa
      361 atttcaaaac aagcatggca ggatagagca gactattatc aaatttcttt tggcttaggt
      421 gatagaggaa aagatacaga aaatcagagc aagtggtatg cctttgccaa ggaagcaatc
      481 gaagaagaac aagctcttta tgtaaaggat aatctgctcc attttgccaa tccacaagga
      541 aaaaatgaac agggagagac actggatacc tatagtccag atgctaatac gctctatgtt
      601 agtcccagtt atttggacaa ggaaaaggtc gtggtagatg ctgagaccaa acagaagtta
      661 gcccatctcc aaaaaggtga gtttatcctc ttgctcccag aacatttgcg ctctcgagaa
      721 gcagaactta agaaagtttt tgaagaaaga ttgagttatt atggaaaatc tggtgaggag
      781 gcaagtgctc ctttggatta tgagatgaaa gcgcacgtta gttatctttc aatgggagaa
      841 aagcggtttg tttataataa cggtgagaat cccgtatcta ctcagtattt gactgatccg
      901 attttagttg tattcacgcc gacttctaca ggtgatagtt ttatatcctt atctagttgg
      961 tctatcaatg ctggaaaaca actctttatc aaaggatatg agagtgggct agaactcttg
     1021 aagaaagctg gaatttatga gcaagtatcc tatcttaaag aaggaagaag tgtttatcta
     1081 actcgttata atgaagttca aactgaaaca gcaactttaa tcttaggagc tattgttgga
     1141 atagctagtt ccttgttact cttttattct gtcaatcttc tatatttcga gcaattccgc
     1201 cgagatatct tgattaaacg aatttcaggt ttacgatttt ttgaaacaca tgctcagtat
     1261 atggttagcc aatttgccag ttttgtattt ggtgctagtc tctttatttt aagcagtcga
     1321 gacttggtga ttggcttgct cactttattg gtctttctag ttagtgcagt tttgacgctt
     1381 taccgtcaag cgcagaaaga atctcgtgtt tctatgacaa ttatgaaagg aaaataggat
     1441 gattgaacta aagaatatat ctaaaaaatt tggaagccgt cagctatttt cagatacgaa
     1501 tcttcatttt gaaggtggga aaatttatgc cttaatcggt acaagtggct gtggtaagac
     1561 aacactcttg aatatgattg gacgattaga gccatatgac aaagggcaaa tcatatatga
     1621 tggcacttct cttaaggaca ttaagccttc tgttttcttt agagattact taggatactt
     1681 atttcaagat tttggcttaa ttgaaagcca aaccgtcaaa gagaatctca atctgggttt
     1741 agttggtaaa aagttgaagg aaaaagagaa aatctctttg atgaaacaag ctctaaaccg
     1801 tgtaaacctc tcttatttgg atttgaagca acctatattt gagttatcag gaggagaagc
     1861 acaacgtgtt gcactagcga agataatttt aaaggatcca cctttgatcc tcgcagatga
     1921 accaaccgct tccttagacc ccaaaaactc tgaggaatta ctttccatcc tagaatcttt
     1981 aaaaaatccg aatcggacca ttattattgc gacccataat cctctgattt gggagcaagt
     2041 ggatcaggtc attcgagtta ccgatttatc acatagatga tatggtaaga ttcagtcaga
     2101 agaaagagtc acaaacacac tttgtggctt ttttatttcc ataaaaatgg taaaatagta
     2161 ggagtagaaa tggagttcga gacatgaaag taatagatca atttaaaaat aagaaagtcc
     2221 ttgttttagg cttggccaag tctggtgaat ctgcagctcg tttgttggac aagctaggtg
     2281 ccattgtgac agtaaatgat gggaaacctt tcgaggacaa tccagctgcc caaagtttgc
     2341 tggaagaagg gatcaaggtc attacaggtg gccatccttt ggaactcttg gatgaagagt
     2401 ttgcccttat ggtgaaaaat ccaggtatcc cctacaacaa tcccatgatt gaaaaggctt
     2461 tggccaaggg aattccagtc ttgactgagg tggaattggc ttatttgatt tcagaagcac
     2521 cgattattgg tatcacagga tcgaacggta agacaaccac aacgactatg attggggaag
     2581 ttttgactgc tgctgggcaa catggtcttt tatcagggaa tatcggctat ccagctagtc
     2641 aggttgctca aatagcatca gataaggaca cgcttgttat ggaactttct tctttccaac
     2701 tcatgggtgt tcaagaattc catccagaga ttgcggttat taccaacctc atgccaactc
     2761 atatcgacta ccatgggtca ttttcggaat atgtagcagc caagtggaat atccagaaca
     2821 agatgacagc agctgatttc cttgtcttga actttaatca agacttggca aaagacttga
     2881 cttccaagac agaagccact gttgtaccat tttcaacact tgaaaaggtt gatggagctt
     2941 atctggaaga tggtcaactc tacttccgtg gtgaagtagt catggcagcg aatgaaatcg
     3001 gtgttccagg tagccacaat gtggaaaatg cccttgcgac tattgctgta gccaagcttc
     3061 gtgatgtgga caatcaaacc atcaaggaaa ctctttcagc cttcggtggt gtcaaacacc
     3121 gtctccagtt tgtggatgac atcaagggtg ttaaattcta taacgacagt aaatcaacta
     3181 atatcttggc tactcaaaaa gccttatcag gatttgacaa cagcaaggtc gtcttgattg
     3241 caggtggttt ggaccgtggc aatgagtttg acgaattggt gccagacatt actggactca
     3301 agaagatggt catcctgggt caatctgcag aacgtgtcaa acgggcagca gacaaggctg
     3361 gtgtcgctta tgtggaggcg acagatattg cagatgcgac ccgtaaggcc tatgaacttg
     3421 cgactcaagg agatgtggtt cttcttagtc ctgccaatgc cagctgggat atgtatgcta
     3481 actttgaagt acgtggcgac ctctttatcg acacagtagc ggagttaaaa gaataaaata
     3541 tgaaaaaaat tgtctttaca ggtgggggga cggttggaca cgttaccctc aatcttttgt
     3601 taatgcccaa gttcatcgaa gatggttggg aagtccacta tatcggggac aagcgtggta
     3661 tcgaacacca agaaatcctt aagtcaggtt tggatgtcac cttccattct attgcgactg
     3721 ggaaattgcg tcgctatttc tcttggcaaa atatgctgga cgtcttcaaa gttggctggg
     3781 gaatcgtcca atcgctcttt atcatgttgc gactgcgtcc acagaccctt ttttcaaagg
     3841 ggggctttgt ctcagtaccg cctgttatcg ctgcgcgtgt gtcaggagtg cctgtcttta
     3901 ttcacgaatc tgacctgtct atgggcttgg ccaataaaat cgcctataaa tttgcgacta
     3961 agatgtattc aacctttgaa caagcttcga gtttggctaa ggtcgagcat gtgggagcgg
     4021 tgaccaaggt ttcggacaaa aacactccag aaccagatga attggtggat attcaaaccc
     4081 actttaatcc caaattgcca actgtattgt ttgtcggcgg ttctgcaggc gctcgtgtct
     4141 ttaaccaatt ggtgacagac cataagaaag aactaacaga gcgctacaat attatcaatc
     4201 taactggaga ttctagcctg aatgagttga gccaaaatct ttttcgtgtt gactatgtga
     4261 ccgatctcta tcaacccttg ttggaattgg ctgatattgt tgtgacacga ggtggtgcca
     4321 atacgatttt tgagctcttg gcgatagcaa aattgcatgt cattgtgccg cttggtcgtg
     4381 aagctagtcg tggtgaccag attgaaaatg cagcttactt tgtaaaaaaa ggctatgcag
     4441 aagaccttca agaaagcgat ttgaccttgg atagtttgga agagaagctt tctcacttac
     4501 taagtcacaa ggaagattac caagctaaga tgaaagcttc taaggaattg aaatctctag
     4561 cagattttta tcaattgttg aaaaaagatt tatcataagg aaagtaaatg tcaaaagata
     4621 agaaaaatga ggacaaagaa atcctcgaag aattgaaaga gttatcagaa tggcagaaac
     4681 gaaaccaaga atatctaaaa aagaaggctg aagaagaagc ggctctagct gaggagaagg
     4741 acaaggaaag acaagctcgg atgggagaag aatctgagaa gtcagaggac aaacaggacc
     4801 aggagagtga aacagaccag gaagattcag aatcagctaa ggaagagtct gaagaaaaag
     4861 tagcatcctc agaggctgac aaagagaaag aagagaaaga agaaccagag tctaaagaga
     4921 aggaggaaca ggataaaaag cttgctaaaa aggctacaaa ggaaaaacca gccaaagcaa
     4981 agattcctgg tatccatatc ttgcgagcct tcacgatttt atttccaagt ctgcttttat
     5041 tgattgtctc tgcctacttg ctcagtcctt atgcgaccat gaaagatatt cgtgttgagg
     5101 gaacggtgca aactacagct gatgatattc gacaggcttc aggcattcag gattcggatt
     5161 atacgattaa ccttctgcta gacaaggcaa aatatgaaaa gcagattaag tctaactatt
     5221 gggttgaatc agctcaactt gtctatcaat ttccaactaa gttcactatt aaggtcaagg
     5281 aatatgatat tgtggcctac tatatttctg gtgaaaatca ttatcctatt ctttccagtg
     5341 gtcagcttga gactagttct gtgagcctga acagtttacc agaaacttat ttatcagttc
     5401 tctttaatga tagtgaacaa atcaaggttt ttgtctcaga acttgctcaa attagcccag
     5461 aactcaaggc ggctatccaa aaggtggaat tagccccaag caaggtgaca tccgatttaa
     5521 ttcgattgac tatgaatgat ttggacgaag tcttggttcc gctttctgaa atgagcaaga
     5581 aactgcctta ttatagtaag attaagccac aattgtcaga accgagtgtg attgacatgg
     5641 aagctggaat ttacagttac actgtggcgg ataaattaat tatggaagct gaggaaaaag
     5701 ccaaacaaga ggctaaggaa gctgagaaaa aacaagaaga agaacagaaa aaacaagagg
     5761 aagagagcaa tcgaaaccaa acgacccagc gctcatcgcg tcgctaggtt taccttttct
     5821 cttatagttc tttagtgacc atgtttttac gtttaatatt tgacatctgt ttttctttat
     5881 gttacatctg caatgtaatc gattacaaaa taatttttga tgaagaaggt aacacatatg
     5941 tcagatgaag tattcttatt tgaagaaatt gaagaaatcg tagcccctac agatggtgaa
     6001 tttttggggg aagttttact tggaactggg gtagttctct taattggagt agcctgttgt
     6061 taaaaagata gggagtgata atcatgcaag ataacttttt atttgaggaa atttcagtac
     6121 cagttagtga tttttcagct ggacttgcaa caggtatcgg atttggttta gcaatccttg
     6181 ctcttgctgg ttgttgaagt ttgttcattt actaacatca agctttttca atttcatttt
     6241 agacagtcat ttaaattttc cgtattagtc ttgcagcaag agattaatag aattagtcat
     6301 tattttattg attgcggact gagggactag agtatgtttt acttaacccc tcttttattt
     6361 attaaaggtt aggtttgtta tgagaattgt tgataagatt aagatattac ctactcctta
     6421 tgagggacac tatcatttat atataccatc cagtaagaaa catgtattag ttgggaaaca
     6481 ggaaaaaaat ggttagagca actaataggt caagaattta ccatatcgg
//
LOCUS       DAVKZB010000096         6073 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKZB010000096 DAVKZB010000000
VERSION     DAVKZB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.096"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            13..168
                     /gene="blpC"
                     /locus_tag="VXA80_001644"
     CDS             13..168
                     /gene="blpC"
                     /locus_tag="VXA80_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV0354108.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(212..1552)
                     /locus_tag="VXA80_001645"
     CDS             complement(212..1552)
                     /locus_tag="VXA80_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0354109.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(1566..2303)
                     /locus_tag="VXA80_001646"
     CDS             complement(1566..2303)
                     /locus_tag="VXA80_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354110.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(2308..2646)
                     /locus_tag="VXA80_001647"
     CDS             complement(2308..2646)
                     /locus_tag="VXA80_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354111.1"
                     /translation="MKYMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2922..3251
                     /locus_tag="VXA80_001648"
     CDS             2922..3251
                     /locus_tag="VXA80_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV0354112.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3425..4474)
                     /locus_tag="VXA80_001649"
     CDS             complement(3425..4474)
                     /locus_tag="VXA80_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354113.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4471..5202)
                     /locus_tag="VXA80_001650"
     CDS             complement(4471..5202)
                     /locus_tag="VXA80_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354114.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5270..5680
                     /locus_tag="VXA80_001651"
     CDS             5270..5680
                     /locus_tag="VXA80_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0354115.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5690..5977
                     /locus_tag="VXA80_001652"
     CDS             5690..5977
                     /locus_tag="VXA80_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354116.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         1998 a         1327 c         1125 g         1623 t
ORIGIN      
        1 gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact aacaactacc
       61 gaactcaacc aaattatagg tggaggatgg tgggaagatt tcttatatag atttaatata
      121 attgaacaaa aaaatacaaa aggatttcat cagccaatac aactataaaa aaacaagacc
      181 aagagaacgc actctcggtc ttattttttc ttcattctat atgtattaca gtaagtacct
      241 gacgaaaaac gtgatcttgg caggtggtat tgagactggt attgggatga ctttccacaa
      301 ttttcataac ggtatagaga ccaactcctc tctcctcccc tttagaactt gctccaaagg
      361 agaagatttc agaaatatcg attccctctt ctttgatgga attttcaatg ataaaggtct
      421 cctgtgctcc atttttaaaa aaggcaattg aaacatgagg ttgacaggct tctacactag
      481 cttcaatagc gttgtcacaa aggatagaca caacggttag aaaatcaagt agactcatcc
      541 cctctacctg aatctcctct ggaacttcga cattaaagac aatgttctta tctctagctt
      601 ttataaattt tccagctagg agacttttga gggcacgatc ccgaacattc accaatcggc
      661 caaggtcata tttattgtcc tgcaattttt cactagaatc ctttaagacc gagtcgtaga
      721 tctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta gtcaagaggt
      781 tggtgtagtc atggcgaaaa ctccgtactt ccttataaag ctcctctata tgccgactat
      841 accgctccat ctctctgtag cgcaagtcct gctcttggtt cagtctctca tggagtttgt
      901 ccttcaaata ggtatccaat ttcttgataa tccccataaa aaagagcagg taaaagacta
      961 ggatgagatg gcgaacagtc gttgattgaa tcccctgttc atattcaaag taagacagat
     1021 tttgtatcac caaatagtaa gcccccatta tccagttaat ctgagtcagg gacttttgaa
     1081 aatctttatc gagaatcccc tttctcaagc tagtgaaatc atagtccaac catttcaaaa
     1141 aagctagaga aatgaagaaa ttgaaaatta ttatacataa ccaaataaat gagtagtcat
     1201 catatacttg cccttgtccc aaaaatggaa gcacaaaata ggaaacacct ctataaaaga
     1261 gattcactaa tatcattgga aagagaccat aaaagagaag gagtttttta ggaagttctc
     1321 ttaacaataa gaaagataag cctatgccgt acaagggttc cataaaataa gataggtaaa
     1381 catttcctac catatagcca agcattccaa aaacaacggc caacagtatc ttaaaaagaa
     1441 aggccttaaa aatcctctca aaagtgagac taattccatc taccttaaag aatatgacaa
     1501 tttttaatcc gtgagtaaca agtgtataca acaatatcca agcaacattc ataaactctc
     1561 ctagctcagt gtaagttatt aatagcctca gacacttccc tgaccttata acgcgcgatt
     1621 agacaacttc caccattggg aaagaaaagc agtttttctt tcttatctaa atgcaccaca
     1681 ttggcaggat tgatgagaaa agagcgatgg cactgcaaga gacgaggctc ctgcttgaga
     1741 acctcctcta gactcgctgt aaattccagc ctgtctgtct tggtatagag aataacacga
     1801 tggggtcttg tggacgtttc gagatagtaa acctctttaa aaggatactg gaattgagca
     1861 aattttgatt taaagtaaaa gcaatcttcc gctagacttt tactgtcttg accattggca
     1921 tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc cttatcaata
     1981 tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga gtgagtcgtc
     2041 acaaagacga ttagggcata tgggtcttgt tcccgaatct ttctagccac ttccagtccc
     2101 ttcatctctt catttcgaat ctcaatatcc aaaaagaata gctgatgggc ccccttctca
     2161 tgcacctctg ccagcagttg gtccggcttg ccaaatacct caaaagagct aggaatgata
     2221 tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg ggaaaaatca
     2281 tcttctaaaa caaatattct catcttctta ctctccctct ttcaaccatt tttgacgtat
     2341 ttctctatag cgacgtctgg catacttgac agcatattga ccttcttctc cgagaaaaag
     2401 gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac aatttcgatg
     2461 acatctcatc aaagtttccc catattggtt ctcaagatta cttaaatttt gaagcatatt
     2521 aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat gtgtttggat
     2581 atagtagata tcgtcgacgt ttactttata gactgttttc tgcgtctgaa taatcatata
     2641 tttcattgga ttctcctcct aagaaaagaa taacagaaaa gttctagccc aagcccaccc
     2701 aatcctaaac agtctttgaa agctcctaaa aggattattt tctacaacat catgatgagc
     2761 ttacaactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
     2821 aagatttgaa taaaaaaaaa gaattaaaag tgataaacta acagtatcaa aacacagttg
     2881 ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat acagacccca
     2941 tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg
     3001 caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca
     3061 aggctgaaac agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca
     3121 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
     3181 cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat gaaaatcaaa
     3241 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaaggcga cgctgacgct
     3301 gacgtggttt gaagagattt tcgaagagta taagtctcta cggatcaacc attatcctgt
     3361 agtaacaaaa aagaagctga gacagactga actcaacttc ttttttagtg tcgtatcagc
     3421 aatgttagtc ctgcatctga cgttttacct gataaggcaa atacaagact agtaaaacca
     3481 aaccagctcc tagtaaggct agaagggcta gtttttcttg gaaggtaatc aacccaacaa
     3541 ctaattccac aagtaaaaca aaactggtca atcctcgaac tacctcctgt aagccttttt
     3601 ccttttgccc cttgtccagc ggaaagagtt gggtcaaata ctggtagtca aaggcatgat
     3661 agagggccag caactggaag agcaagaggt agttaaagag aactaccact gctgtcgcaa
     3721 tccaagcttg ctcgataaaa acctgcgcca gtacggaaag taagagaaga cgaagactga
     3781 gagcaaagag gtcgccattt cgcagataag aacgcagata gagattttgc caaatctttc
     3841 cagacacctt ctgaacagcc tttaagataa agtccagata ggcacgacgt ttgacactgt
     3901 ttgaaattcc ctttacctgc gtaaagaggg caaagaaacg aagcaagact tgcttacgct
     3961 tgctttcttg ggaaatgaca tagtcccagt ccagcccagt ttcagtgaaa aatttgctgg
     4021 ccttttgacg aaagtggaaa tattttccta cccccaataa aagcacatag agcagaaaaa
     4081 ctggcaagcc ataacccatt gctaaaaata aaggcgcaaa taacagcaag aaaagggtct
     4141 gtacaaagag ccaaaagact agggaaatgc cagtttgacg cttgagatgg agcttgattt
     4201 cctcttctcc aactaagaga aagagcttgt ctggagcctc catataggtg gcaattcctc
     4261 cccaaagtaa aagtaaaaca gacgtaattc ctacaaacaa aaggataggc caatgatttt
     4321 caggaaaatg ttgtaagagt tgactgtact ggtaggctag aaagcccaac aggacaagca
     4381 ggaacaagac aaagtggtca ttgagcacat agcgcagata accaaggcac tccttacgaa
     4441 aggcctgctt tctctttaaa aacaagtctt tcatagatcc tcctctttgg tcagagccaa
     4501 gtaaatatca ttcaaactag cctcaggcat atcaaaggct tcacgtagtt gcaggagatt
     4561 gcctttggca cgcacctctc ccttgtgaag aatgacaaag gcatcacaca tcttctccgc
     4621 cgaatccagc acgtgggtac tcatgagaat agacttgccc ttttgcttct ccacttccaa
     4681 aagctgaatc aaatcagaaa tagccagcgg atcaagacca aggaaaggct catccacgat
     4741 gaaaagactt ggatccacca caaaagcaca gataatcatg accttctgct tcatcccttt
     4801 tgaaaaatga acagggaacc agtctaattt ctgttccaaa cggaacattt ttaacaaggg
     4861 ctctactcgt tcgaaagcca ctttttgctc aataccgtaa gccatagcaa ccgtttcgat
     4921 atgctctctg agggtcaatt cctcatacag actaggcgtc tcaggaatgt agccaatctg
     4981 cttgcggtag ctagtcgcat ctccttgcag agtcaggcca ttgatattga tggagccact
     5041 ataaggtgcc aacagaccga taatctcatt gatcgtcgtt gatttcccag caccattgag
     5101 accaatcaaa ccgaccaact gcccactttc aacagtaaag gacacatctt tcaaaacagg
     5161 gacatgaaca tagccacctg tcaggttttt aatttctaac atattttctc caaatctggt
     5221 ataatgtagc tatattatat caaaattcag tacagtagag gtagatttta tgtcagattg
     5281 cattttttgt aaaatcatcg caggggaaat tcctgcttca aaagtatatg aagatgagca
     5341 ggtccttgcc tttcttgata tctctcaagt aacactagga cacaccttgg tcgtgccaaa
     5401 agaacactat cgcaatcttt tggagatgga tgctacgagc gccagccaac tctttgccca
     5461 agtaccaaaa gtagctcaaa aagtcatgaa agtcactaag gctgctggta tgaatatcat
     5521 ttccaactgt gaagaagtcg ctggtcaaac agtttttcat actcacgttc accttgtacc
     5581 tcgctacagt gctgacgatg acctcaagat tgattttatc gcccacgaac cagactttga
     5641 caaacttgct caagtcgctg aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc
     5701 caacctactg ctatttgcag gagctgcagc cggaagttat ctggtcacaa aaaatcgcca
     5761 aaccatcaca gatgaagtct tgaataccac tgaccgcgtt caagctatca aggacgatgt
     5821 ggatattatc caaaacagcc tgcaaatcat tgaccagcaa aaagaactta tcaaggaata
     5881 ccaagaagac ttgacttaca agtttaaggt cttggaaaag gatatccaga ctagactagc
     5941 tgtgataaaa gaaatgcagg gaactgaaga taagtaaaaa gagccccccg gctctttttt
     6001 aaaacgtaaa caagttatag tgaattgaat ttagaatagc acatcatagt ttctaaagca
     6061 ttgttagaga tta
//
LOCUS       DAVKZB010000097         6064 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKZB010000097 DAVKZB010000000
VERSION     DAVKZB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.097"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            18..1045
                     /locus_tag="VXA80_001653"
                     /pseudo
     CDS             18..1045
                     /locus_tag="VXA80_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1346..3235)
                     /locus_tag="VXA80_001654"
     CDS             complement(1346..3235)
                     /locus_tag="VXA80_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV0354117.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKDQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            3373..4938
                     /locus_tag="VXA80_001655"
     CDS             3373..4938
                     /locus_tag="VXA80_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0354118.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4938..5678
                     /locus_tag="VXA80_001656"
     CDS             4938..5678
                     /locus_tag="VXA80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0354119.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEILKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5787..6002)
                     /locus_tag="VXA80_001657"
     CDS             complement(5787..6002)
                     /locus_tag="VXA80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354120.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1872 a         1368 c         1100 g         1724 t
ORIGIN      
        1 tatctggagg tactcccatg ctttatgtgg gcattgatgt cgctaaaaat aaacacgatg
       61 ttacagcctt gaatgttcga ggaaaactgt tcttaaacca ctcacttttt caaataataa
      121 agctggtttt gaactattag atctgtctct tcgacagctc aaccaagact gtctcatcgc
      181 tcttgaagat accggacatt atgcctttaa tctactgaac ttcctgcatg aacaaggata
      241 taaagtttac acctacactc ctttgctcat caaggaattt gccaaatcat tatcacttcg
      301 caagactaaa acggataaga aagacgctca tggcatcgct cttaaacttc tttctgaccc
      361 caatcgtgaa caatttcaac acgataatcg gcaagtagaa ctaaaaatac tggctagaca
      421 tattcatcgt ctcaagaaaa aacagtctga ttggaaagta caatacactc gttgtcttga
      481 tatcatcttt cctgagttgg ataaaatcgt tggaaagcat tcagaatata cctaccaact
      541 cttgacatgc taccctaatc ctcagaaaag gcttgaggca ggatttgata agctgataga
      601 aattaagcga ttgaccgctt ctaaaattca ggatatcctc tcagtcgcac ctcgttctat
      661 cggaacaaca tctcctgctc gtgaattcga aatcatcgaa aacatcaaac attacaagag
      721 gctcattgac aaggcggaaa aatgtgtcaa tgacttgatg gctgagttca actcggtcat
      781 cacgacggtt actgggattg ggaatcgttt aggggcggtc attttagccg agattcaaaa
      841 tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg attcttctat
      901 ttatcagtca ggtcagattg atttagctgg aagaatggtc aaacggggtt ctcctcatct
      961 gcggtgggca ctcatacaag ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc
     1021 ctatcttaag actaagttag aataagaaaa acattacaat gtagccatca tccaccttgc
     1081 aaaaaaactt atccgaaccc tgttttatat cctaaaaaag agctgccatt tgacgaacaa
     1141 aaagtgatgt aatttctgaa atcaacacag gattaccttt gaaaaaatgg actttttcaa
     1201 gggctttttg ccgtgctcta aaatcatcgc caaatcatca aattgaaata agaaatagtt
     1261 tagataaaat tattgacttt tcttatagtt tgtctttctt ctatcttcca ttatatcata
     1321 ctttttcagt ctatggtagc tctctttaaa aggctccgat actgccgcca cctccgcctc
     1381 cagagaagcc accgccagaa cttccacttc cagaagatac ggagtaggta cttgctgtat
     1441 ttgcgacact agcataatgg ctcatttgcg cgcttgaatg ataaaacata ctgtgccagc
     1501 cataagctac atagagattg atatctggat tttcaacttg aatctgatga accttcatca
     1561 aatgactaac cttgtccgca tagccaaata aggttgcata gaccaagaga cgattccaaa
     1621 caacaatact ttccaattca gccttatcca gtcgtgcaat ctcacgtaac atattttcaa
     1681 aactggtcca gagatagtag acctcagctc ccgcttcatt tagaacacca tcacgattat
     1741 ctagtcgaag cttccaataa tagaaaacag acaaaactaa ccctagaaaa ccaagtattg
     1801 gcaaagggag gtaaagatag ccatgaacgt ctaaactgta caagaacaaa ccaaatccga
     1861 taaatagggg caggatagtc aaggcaccca tacccacttg caaggccttt tccccaccag
     1921 ttaaaggacg ataataatct gggagccccc agaaggaaac tcgttttctc actccttctt
     1981 gcatctggtt caatacctct tcaaaagaag atttgagttg aagccctctt gcttgaatcc
     2041 gtttttcatc agaaactttg gctctacgat aaagactatc agataccttg taatccgcaa
     2101 acaaattgga aagagtttct tcttttttac ctgaaaaagc tagatttagg cagtctttct
     2161 caaagcttga caaaccatct tcttttacta gcctcaaacc aactgcatct ccttctgaaa
     2221 tgatagagac attcccacgg tctatcacat ctagcaaggt agcttgaata agttgatcaa
     2281 aggtgaattt tccagctccc ttgaccaagg gactcacttc ctccaaggag gtcgagtaga
     2341 ctgcttctga taaaaccata ggctctaatt ccattggggg ttcatagaga cgatgatttt
     2401 tagcatattt gattgaagga gcggtctttc ttctatagat aaaatagaaa cagatgctca
     2461 ataacaagga gatggaaagg atcgaaggga agatccaagt aagtagttgt ttactttgat
     2521 ctttttcttt aacgatcgaa tcctctatct tattaaactc ttctaaacga ttccctttca
     2581 agccctgatc cctagcgcta gcaaaatcag ttcgcggcca gtaggcatgc aattcaactc
     2641 cacgcttagc cggaagattg tctaaacgaa tagtataatc aaggttactc ttttcaaccg
     2701 ttctctctct aaaaagtttc cctgtatgga aaaagagttt ctcagccccc ttgtctcccc
     2761 ttacatgaaa ttcaaacttt tcaatagact ctgaactatc tgtcagaggt tgccaattta
     2821 attcagcgat atcatcataa aggaaaagta aattttttaa gttccagacg aggtcaactt
     2881 caactatgtc gccctcctga cctggattat agactctcac agtataacca tccgcttctt
     2941 ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt tttcgcggcc tgaatctttg
     3001 gatgagggtc aatgtcaaac ccgctaggca tcttaccagc acgtccaagt cccacgattt
     3061 ggcccttaaa gtcctcctca aactggtaaa ctatcttctg tctaaactct gccgtattgt
     3121 ctgcatgaat atacaaatcc ccttgataag agtttatctt gaaatcaatg gcaaaaacag
     3181 agagtggaag aaggcaaaac aagcctaaca ccagtaagaa aaaagttttt ttcatcaact
     3241 aagccccctt tttatctttc ttatcattat agcatttttt cgtaagtaaa ggcttactta
     3301 tgttgaaaaa ttacgcattt ttcgataaaa taggagacag ttatttttta atagatcaga
     3361 ataggagaaa tcatgagaaa aatatactta tctattttca caagtctctt gctgatgcta
     3421 ggacttgtca atgttgctca agccgatgaa tatttacgca tcggtatgga agcagcatat
     3481 gctcccttta actggaccca ggatgatgat agcaacggag ctgtcaaaat cgatgggacc
     3541 aatcagtatg ccaacggata cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt
     3601 aaagaacctt tggttgttaa aaccaagtgg gaaggtctag tccctgccct tacttctggt
     3661 aagattgaca tgattatcgc aggtatgagt ccaactgcag aacgcaaaca agaaattgcc
     3721 ttttcgagca gttactatac tagcgaacca gttttgcttg tcaaaaaaga ttctgcctac
     3781 gcaagtgcta aatctttgga tgactttaac ggtgcaaaaa tcacttctca acaaggggtc
     3841 tacctttata acttgattgc acaaatccca ggtgctaaaa aagaaacagc catgggagac
     3901 ttcgctcaaa tgcgacaagc tcttgaggct ggtgtcattg atgcttatgt ttctgaacgt
     3961 ccagaagcac tgactgctga agctgcgaac tctaagttca agatgattca agtagaacct
     4021 ggtttcaaaa ctggggaaga agatacagct atcgctatcg ggcttcgtaa aaatgacaat
     4081 cgtattagcc aaatcaatgc cagcattgaa accatttcaa aagatgacca agttgccttg
     4141 atggatcgta tgatcaagga acaacctgcc gaagctacaa caactgaaga gactagcagt
     4201 agtttcttta gccaagttgc taaaattctt tctgaaaact ggcaacaact cttgcgtggt
     4261 gctggtatca ctcttttaat ctctatcgtc ggaaccatca taggtctcat tattggactt
     4321 gccattggtg tcttccgtac tgctcctctc tctgaaaaca aagtcattta cggcctacaa
     4381 aaactagtcg gctgggttct caatgtctac attgaaattt tccgtggtac gccaatgatt
     4441 gttcaatcga tggttatcta ctatggaact gcccaagctt tcgggatcaa ccttgaccgt
     4501 acactggctg ctatcttcat cgtttcaatc aataccggtg cctacatgac tgaaatcgtc
     4561 cgtggtggta tcctagcagt tgacaaggga caatttgaag ctgcgactgc tcttggtatg
     4621 acccataacc agaccatgcg taagattgtc ctacctcagg tagtccgtaa catcctacct
     4681 gcaactggta atgaatttgt catcaatatc aaagatacat ctgtattgaa cgttatctct
     4741 gttgtcgaac tttatttctc aggaaatacc gtggcaacac aaacctatca atacttccag
     4801 acatttacaa tcatcgccgt gatttacttt gtcctcacct tcaccgtaac acgtatccta
     4861 cgctttatcg agcgcagaat ggacatggat acctacacta caggtgctaa ccaaatgcaa
     4921 acggaggatt tgaaataatg acacaagcaa tccttgaaat taaacacctc aaaaaatcct
     4981 atggacaaaa cgaagtgcta aaagacattt cactcactgt ccacaaggga gaggtcatct
     5041 ctatcatcgg aagctctgga agcggaaaat cgaccttcct acgctccatt aacctacttg
     5101 aaacaccaac tgatggacaa atcctttatc atggacaaaa cgtcctcgaa aaaggctatg
     5161 acctcacgca ataccgtgaa aagttgggga tggttttcca atcctttaac ctctttgaaa
     5221 atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac tgtcctaaaa cgcgaacgca
     5281 cagaagctga aaagattgcc aaagaaaacc tggaaaaggt cggcatggga gaacgctact
     5341 ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca acgtgtggcc atcgctcgtg
     5401 ccctctccat gaatccggac gctattctct ttgatgaacc aacatcagct ctcgatccag
     5461 aaatggttgg agaaatcctc aaaatcatgc aggacctggc tcaggaaggc ttgaccatga
     5521 ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg
     5581 ataagggcgt gatcgctgaa gaaggtaaac cagaagacct cttcaccaat cctaaagaag
     5641 accgaacaaa agagttcctt caacgctatc tcaaataaaa agaaaaggct gcatcaaccg
     5701 tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc tctatctcta ctacttgaag
     5761 gtcaagaatc cgccgttatc caaagattaa tcttcaaatt tttcataatt tttttcatag
     5821 aaatcaatta ggcctagggc tgtttcaaat gaaatatttt ttacttttgc acgaccctgc
     5881 gccagagcaa tgatagacat ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg
     5941 attttcttat cacgaaccca gccaacaact gattctactt tttcaaagtt tgacttagcc
     6001 atgtttttct cctattttct tctttataat atttaattgt atacgttttt actacttttg
     6061 tcaa
//
LOCUS       DAVKZB010000098         6014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKZB010000098 DAVKZB010000000
VERSION     DAVKZB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.098"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            44..1744
                     /locus_tag="VXA80_001658"
     CDS             44..1744
                     /locus_tag="VXA80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV0354121.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1737..2213
                     /gene="ilvN"
                     /locus_tag="VXA80_001659"
     CDS             1737..2213
                     /gene="ilvN"
                     /locus_tag="VXA80_001659"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV0354122.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2279..3301
                     /gene="ilvC"
                     /locus_tag="VXA80_001660"
     CDS             2279..3301
                     /gene="ilvC"
                     /locus_tag="VXA80_001660"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0354123.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGLRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3408..3644
                     /locus_tag="VXA80_001661"
     CDS             3408..3644
                     /locus_tag="VXA80_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354124.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3829..3969
                     /locus_tag="VXA80_001662"
                     /pseudo
     CDS             3829..3969
                     /locus_tag="VXA80_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            4019..5269
                     /gene="ilvA"
                     /locus_tag="VXA80_001663"
     CDS             4019..5269
                     /gene="ilvA"
                     /locus_tag="VXA80_001663"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV0354125.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1845 a         1014 c         1385 g         1770 t
ORIGIN      
        1 atgatagtaa caagttattt ttaagaggaa agaaagggga ataatggaga aaatcagttt
       61 agaatctcct aagacggggt cggacctagt tttggaaaca cttcgtgatt taggagttga
      121 taccatcttt ggttatcctg gtggtgcggt tttgcctttt tatgatgcga tatataattt
      181 taaaggcatt cgccacattc tagggcgcca tgagcaaggt tgtttgcatg aagctgaagg
      241 ctatgccaaa tcaactggaa agttgggtgt tgccgtcgtc actagtggac caggagcaac
      301 aaatgccatt acagggattg cggatgctat gagcgatagc gttccccttt tggtctttac
      361 aggtcaggtg gcgcgagcag ggattgggaa ggatgccttt caggaggcag acatcgtagg
      421 aattaccatg ccaatcacta agtacaatta ccaagttcgt gagacagctg atattccgcg
      481 tatcattacg gaagctgtcc atatcgcaac tacaggccgt ccagggccag ttgtaattga
      541 cctaccaaaa gacatatctg ctttagaaac agacttcatt tattcaccag aagtgaattt
      601 accaagttat cagccgactc ttgagccgaa tgatatgcaa atcaagaaaa tcttgaagca
      661 attgtccaag gctaaaaagc cagtcttgtt agctggtggt ggaattagtt atgctgaggc
      721 tgctacggaa ctaaatgaat ttgcagaacg ctatcaaatt ccagtggtaa ccagtctttt
      781 gggacaagga acgattgcaa cgagtcaccc actctttctt ggaatgggag gcatgcacgg
      841 gtcattcgca gcaaatattg ccatgacgga agcggacttt atgattagta ttggttctcg
      901 tttcgatgac cgtttgacgg ggaatcctaa gactttcgct aagaatgcta aggttgccca
      961 cattgatatt gacccagctg agattggcaa gattatcagt gcagacattc ctgtagttgg
     1021 agatgctaag aaggccttgc aaatgttgct agcagaacca acagttcaca acaatactga
     1081 aaagtggatt gagaaagtca ctaaagacaa gaatcgtgtt cgttcgtatg acaagaaaga
     1141 gcgtgtggtt caaccgcaag cagttattga acgaattggt gaattgacga atggagatgc
     1201 cattgtggta acagacgttg gtcaacacca aatgtggaca gctcagtatt atccctacca
     1261 aaatgaacgt cagttagtga cttcaggtgg tttgggaaca atgggctttg gaattccagc
     1321 agcaatcggt gctaaaattg ctaacccaga taaggaagta gtcttgtttg ttggggatgg
     1381 tggtttccaa atgaccaacc aggagttggc tattttgaat atttacaagg tgccaatcaa
     1441 ggtggttatg ctgaacaatc attcactcgg aatggttcgc cagtggcagg aatccttcta
     1501 tgaaggcaga acatcagagt cggtctttga tacccttcct gatttccaat tgatggcgca
     1561 ggcttatggt attaaaaact ataagtttga caatcctgag accttggctc aagaccttga
     1621 agtcatcact gaggatgttc ctatgctaat tgaggtagat atttctcgta aggaacaggt
     1681 gttaccaatg gtaccggctg gtaagagtaa tcatgagatg ttgggggtgc agttccatgc
     1741 gtagaatgtt aacagcaaaa ctacaaaatc gttcaggagt cctcaatcgc tttacaggtg
     1801 tcctatctcg tcgtcaggtt aatattgaaa gcatctctgt tggagcaaca gaagatccga
     1861 atgtatcgcg tatcactatt attattgatg ttgcttctca tgatgaagtg gagcaaatca
     1921 tcaaacagct caatcgtcag attgatgtga ttcgcattcg agatattaca gacaagcctc
     1981 atttggagcg cgaggtgatt ttggttaaga tgtcagcgcc agctgagaag agagctgaga
     2041 ttttagcgat tattcaacct ttccgtgcaa cagtggtaga cgtagcgcca agctcgatta
     2101 ccattcagat gacgggaaat gcagaaaaga gcgaagccct attgcgagtc attcgcccat
     2161 acggtattcg caatattgct cgaacgggtg caactggatt tacccgcgat taaaaatcca
     2221 acttaaattt gttaaaccag cttaaaaggc aataaataat agaaaagaga gaaaagctat
     2281 gacagttcaa atggaatatg aaaaagatgt taaagtagca gcacttgacg gtaaaaaaat
     2341 cgccgttatc ggttatggtt cacaagggca tgcgcatgct caaaacttgc gtgattcagg
     2401 tcgtgacgtt attatcggtg tacgtccagg taaatctttt gataaagcaa aagaagatgg
     2461 atttgatact tacacagtag cagaagctac taagttggct gatgttatca tgatcttggc
     2521 gccagacgaa attcaacaag aattgtacga agcagaaatc gctccaagct tggaagctgg
     2581 aaacgcagtt ggatttgccc atggtttcaa catccacttt gaatttatca aagttcctgc
     2641 ggatgtagat gtcttcatgt gtgctcctaa aggaccagga cacttggtac gtcgtactta
     2701 cgaagaagga tttggtgttc cagctcttta tgcagtatac caagatgcaa caggaaatgc
     2761 taaaaacatt gctatggact ggtgtaaagg tgttggagcg gctcgtgtag gtcttcttga
     2821 aacaacttac aaagaagaaa ctgaagaaga tttgtttggt gaacaagctg tactttgtgg
     2881 tggtttgact gcccttatcg aagcaggttt cgaagtcttg acagaagcag gttacgctcc
     2941 agaattggct tactttgaag ttcttcacga aatgaaattg atcgttgact tgatctacga
     3001 aggtggattc aagaaaatgc gtcaatctat ttcaaacact gctgaatacg gtgactatgt
     3061 atcaggtcta cgtgtaatca ctgaacaagt taaagaaaat atgaaggctg tcttggcaga
     3121 catccaaaat ggtaaatttg caaatgactt tgtaaatgac tataaagctg gacgtccaaa
     3181 attgactgct taccgtgaac aagcagctaa ccttgaaatt gaaaaagttg gtgcagaatt
     3241 gcgtaaagca atgccattcg ttggtaaaaa cgacgatgat gcattcaaaa tctataacta
     3301 attagaaata tatagcgctg gagatgattt tatgaaaaag attatgagaa aaattgcatc
     3361 gttattattg gttctagttg tataatgtaa ttacaccgtc ggtaatagtg ctagcagacc
     3421 aaaataaagc agattggtcg tatgatgaaa atgctgtaat taacatttat gatgatgcta
     3481 attttgaaga tggtaggttg catatgaact ttgaacaatt cttcaaattg gcacaaatat
     3541 ctagagaaga aggtcttgaa attcattctc cgtttgagag agctggtgcg actaaatctg
     3601 ctcgttatat agcgaaatgg attttgagaa ataaaaaaca ttaacaaata tagttggtaa
     3661 atcattagga cctaaatcag ctgttagatt cggagaagct ttatcctata ttgaaggtcc
     3721 tcttcgcaga ataaatgaga cgatagatgg cggtttatat caaatagagc aaattattgc
     3781 atctggattg aaagaatcgg gtttaaatga ctggactgcg aaaactttcg taatgatagt
     3841 gaagaaagtt tggtagaatg gttatatgat tggatttcca caaatcgtta tgaacttcct
     3901 aaagagtttt cgattcgtat ggctcataaa taccatgaaa gtgttactga agttttcgga
     3961 gatgaataac taaaaaacag tcattagtga ctgtttttta tagaaaaaga ggttttatat
     4021 gttaagttca aaagatataa tcaaggctca caaggtcttg aacggtgtgg ttgtgaatac
     4081 tccactggat tacgatcatt atttatcgga gaagtatggt gctaagattt atttgaaaaa
     4141 agaaaatgcc cagcgtgttc gctcctttaa aattcgtggt gcctattatg ccatttccca
     4201 gctcagcaag gaagaacgtg aacgtggggt agtctgcgct tctgcgggaa atcatgcgca
     4261 gggagtagcc tatacttgta atgaaatgaa aattcctgct actatcttta tgcctattac
     4321 tacgccacaa caaaagattg gtcaggttcg cttttttggt ggggattttg taactattaa
     4381 actagttgga gatacctttg atgcctcagc caaagcagct caagaattta cagtctctga
     4441 aaatcgtacc tttattgatc cttttgatga tgctcatgtt caagcaggtc aaggaacagt
     4501 tgcttatgag attttagaag aagctcgaaa agaatcgatt gattttgatg ctgtcttggt
     4561 tcctgttggt ggtggcggtc tcattgccgg ggtttctacc tatatcaagg aaacaagtcc
     4621 agagattgag gttatcggag tagaggcgaa tggagcgcgt tccatgaaag ctgcctttga
     4681 ggctggaggt ccagtcaaac tcaaggaaat tgataaattt gcggatggga ttgctgtgca
     4741 aaaggtaggt cagttgacct atgaagcaac tcgtcaacat attcaaactt tggtaggtgt
     4801 cgatgaggga ttgatttctg aaaccttgat tgacctttac tctaagcaag ggatagtcgc
     4861 agaacctgct ggagcggcta gtatcgcctc tttagaggtt ttagctgaat atattaaggg
     4921 gaaaaccatt tgttgtatca tttctggagg aaataatgat atcaaccgta tgccagaaat
     4981 ggaagagcgt gccttgattt atgatggtat caaacattac tttgtggtca atttcccaca
     5041 gcgtccagga gctttgcgtg agtttgtaaa tgatatcctg gggccaaatg atgatatcac
     5101 acgttttgag tatatcaaac gagctagcaa gggaacaggc ccagtattga ttgggatcgc
     5161 tttggcagat aagcatgatt atgcaggttt gatccgtaga atggaaggtt ttgatccagc
     5221 ttatattaac ttaaatggta atgaaacgct ttataatatg cttgtctgag gactaataaa
     5281 aaaatatcat attatttgta caactgatgt atatgtgcta taatgaagat gaagaaaggg
     5341 gacgattcct atggttttag aaaacttatc gtgcaattct tacttctgtt cattatagca
     5401 cttatgatgt ttggctcaga tttccatcct tcattttgat ttcctatcta ttgacaagca
     5461 tagtcacact gtctttaata ctcttcgaaa atctcttcaa accacgttag ctctatctgc
     5521 aacctcaaaa cagtgttttg agcaacttgc ggctagcttc ctagtttgct ctttgatttt
     5581 cattgagtat aaggtatgat ttgatttctt tttgttgaca aatataccat attaaaagga
     5641 tatataagta attaactgag tttatctgtc ttgtcatctt tattaaggat ggtttagata
     5701 atcgggtgtc tgcttctagg ctagcacctc aatatccaaa ggagtgatga atttgaagga
     5761 tataaggaat acctatctct cagatgattt attgaggaag aaagatagga gtttttgagc
     5821 tagtgaaggc ttggatttct aaaggttaga actatcatct tcagttctta aatcgaagaa
     5881 ataagctatc ttacggaaat agagaagcat tttttaagaa cttgaataat ttcgcacctt
     5941 aagagggtaa taatacagta tttttattag caaatattta tggtgtagag gctagcaaaa
     6001 cctatatatt atcg
//
LOCUS       DAVKZB010000099         5952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKZB010000099 DAVKZB010000000
VERSION     DAVKZB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.099"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            18..1651
                     /locus_tag="VXA80_001664"
                     /pseudo
     CDS             18..1651
                     /locus_tag="VXA80_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(1681..4233)
                     /locus_tag="VXA80_001665"
     CDS             complement(1681..4233)
                     /locus_tag="VXA80_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV0354126.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMILPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPITVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4295..5917)
                     /locus_tag="VXA80_001666"
     CDS             complement(4295..5917)
                     /locus_tag="VXA80_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354127.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNISELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1994 a          987 c         1101 g         1870 t
ORIGIN      
        1 ttaaaaaagg agaaactatg tttaaagaat ggaaagcaat ttaaaaaaaa ccaaccttta
       61 ttattgtcat gatcgggatt tctcttattc cagctctgta caatatcata tttttgtcat
      121 caatgtggga gccatatggg caattgtctg acttacctgt ggcagttgta aataatgata
      181 aagaggcttc ctataatggt aatactatgg caataggaaa agacatggtg tccaatttaa
      241 aagaaaataa aaccttggat tttcattttg tagatgaaga ggaaggaaag aagggattgg
      301 aagatggcga ttactatatg gtagtgactt taccaagtga tttatctgaa aaaacaacta
      361 cattatccaa tattcaatcg acagcagctt atcaatcatt gacaagtgag caacaaactg
      421 agataagtga ttctgtatct caaaattcaa ctgatagtat tcaatcggct cagtcaattg
      481 tagctttagt acaaggttta cagggaagtt tagaaaactt acaaaatcaa tcttctaatc
      541 tttcgacttt aaaaaatcaa gctaatcaag tattacctat tacttctact tctttgatag
      601 gattgtcaag tggattaaca gagatacaag gagctgttac tagcaaatta gttcctgcca
      661 gtcagtcgat tgcatcaggt gtaaacgcat atactacagg tgttgataaa gtttctcagg
      721 gcgcaagtca actaagtgaa aaaaatgcca ccttgacagg tagtttggat caactagttt
      781 caggctcaaa caccttgaca caaaaatctt ctagattgac agcaggagtt gataaattac
      841 aatcagaatc tgggcaatta gcagacaaat ccagtcagtt actttcaggt gcttctccat
      901 tagagaatgg agctaataaa ttggcagatg gatctaggaa actagcagaa ggtggaacaa
      961 agttaacttc tggattggaa gatatacaga caggacttga taatgctagt gatcaactca
     1021 aatcagcatc aacagaatct aaaaatgcag agattttgcc aaatccactc aatctttcaa
     1081 aaacagacaa tgatcaagtt cctgtaaatg gaatcgcaat ggctccttat atgatatcag
     1141 ttgctctttt tgttgcagca atatcaacaa atatgatatt ttcgaaattg ccttcaggac
     1201 gtcatccaga gagccgttgg gcttggttga aatctcgagc tgaaataaat ggtattatag
     1261 ctgttttggc aggaattttg gtatatggag gagttcatct tattggttta actgctaatc
     1321 atgagatgag aatatttatt ctcatcatcc taacaagttt agtattcatg tctatggtga
     1381 ccactttaac aacgtggaat agccgtatag gagctttctt ctcacttatt ttgcttttac
     1441 tacagttagc atcaagtgca ggtacttatc cacttgcttt gacaaatgat ttctttagat
     1501 ctattaatcc ctggttacca atgagctatt cagtttcggg attacgacaa acaatctcca
     1561 tcaacaagtc attttcctag ctgtcatact agctctattt actggtttag gtatgctagc
     1621 ctatcaacat aagaaaatgg aagaagatta aaaaaatcga ccgattaact ggtcgatttt
     1681 ttatgcctta gatgactttc gtctgtgatt atagattcca aatagtaaga gagaagtaaa
     1741 ggaacagatt gctccagtaa taaaaccatt gggaatgaag gaaagtgtaa tagttccttt
     1801 ccccttggga atgtcaactt tcataaatcc agtttgagct tgtttaattt ctattttctt
     1861 accatcttgg taggcagacc aacctttgtc ataaggaatg gtgaagaaaa tagatgtatc
     1921 ttgttggaca tcatatgtag caaaaacctt gtttttagaa gttgatactg tgataggttg
     1981 ttctttaatt ttttgaattg cctcggtgaa agttttggta tctaaacgat agaaggtagg
     2041 agattcaaat gatacttgtg aatttccagg gaaactaaca ttgatattga aagttttttt
     2101 ctctttagta tatcctagat taaagaagga gaagacatta tcagttgtaa aagtcttttt
     2161 ttcaccattt acaaggatgt caaccttctt ttgtttatcg ttagaaaagt gaaggtttat
     2221 gaaagagaga taaacttggc tgttttctgg aacttcaatt tgatactgga ttgctgcatc
     2281 ttcatttgaa gaacttgtga cactaatcaa atcattagta ttttctattt tttctgtttt
     2341 ttcataaggt attggagaaa aataatcaaa attgacgtta gcaagttgat ttaaaaatga
     2401 ggcctgatta tccaaggtat gttcattgaa cttgacatca ttgtaaacag attgactagc
     2461 aactgcaatc ggaagagagt attgattttc atatagggta agattatctt tttgatagat
     2521 atctttaaag ccatacttat caataggact gtctgagata ttgtactgga taccaaataa
     2581 actatcagcc aaaatactat tatttgcata tcggagattg agattagtcc cagaggattt
     2641 aaaaccaagt ttatctaaag tagagcttga tgaacgattt cgaacagatg aaaattgaga
     2701 gattccattg tagttgaatt tcatactgtc atttcctgtc tgagtttgta gtttttcagt
     2761 acgagtaaat tgatttccaa tatatgttga gaaagattcc atagctggga tatctcgact
     2821 ataagcactt cgagaagcaa atccccattc cttagcaatt ccgtccattt gagatgaagc
     2881 atttaaactc atttcaacca gtataaataa agagattaga atggcaaata gattcacaga
     2941 tataaacttt ttgataactg caaggagtaa aagagaatag acaaccaaaa attcaagagt
     3001 aagcagaata ttcaaatctg ttaaaaaaga ataatgcgat tttagataga tggtagctaa
     3061 aaatcctgtt actacaagaa aaagcgaaac taaaaaattc cagactttaa gttctttcag
     3121 acgctttaag acttctgctg ctgtgtaaat taacaaggta gagaaaatcc aagcatagcg
     3181 atgtaaaaac atgtttggag tatgcatgcc ttgccaaaat aagtcaagag cttctatgta
     3241 aaagcttgca attagaaatg caaagaatat tacatatatg agtttcacgt gaaacttaat
     3301 agatttcagc gtaaaaaata aaatggtcaa aataaaggga agtagtccaa caaaaatcat
     3361 tgggatggcc ccatactttg ttgtgtcaaa ggaaccaatg aattgcttag caaagagatc
     3421 aagataccag ctactttcag tttgaaactt tgtaacttca gtcaattttt ccccatgtgt
     3481 ctgtaaatca aacagagtgg gaagaatcat aatcaaacta gccataccgg ctaaaaagga
     3541 gataactata aaatcaagaa cagatgattt tcgagtctta aagtcccacg aaatttgaca
     3601 gagataccag aaaataagaa acaatactgt catatatcca aaataataat tttgaataaa
     3661 taagattgac agacttgtaa agtacaatag gagtttcttt tcagttatca gtagatgtaa
     3721 accagttata attaaaggaa tcaagataaa aacatctagc caggttttta tctctaattg
     3781 actgacagtg aaactcatca gagcatagga agtagataag gctagtttta aagtctgagg
     3841 gatagattga aacaatttat tcaaactaaa aaaggttgac agaccaatca atccaaattt
     3901 taagagagtt gtcagataga tagcatctgg catattcgtt agatcaaaaa agtaaactag
     3961 aggcgcgaga aaactaccca agtaataact agatagggca tagaagttta gccctagacc
     4021 acttgtaaag gtgtaaaaca gactactatt tccatgtagg atatttcgta aggctacatc
     4081 aaaaataacg tattgatgaa agccatctcc taatagagga gagttgtcgc tattccagta
     4141 gataccttga gatagatata ctccagacat aatcactaca ggaatgatga aagaaataaa
     4201 ataggttcga tatgttttta aaaatgattt catgttacct cgtagaatga tagaaaactc
     4261 agttggttaa cccaactgag ttttgaagtt ttatttagtc tttccaaagt tctttaactt
     4321 ttgcttgtac ttctgcattt tctaggaatt catcgtaggt ttcatcgata cggtcaatga
     4381 cgccattttt agataagaca atgatatggt tagccaaagt ttgaataaac tcgtggtcat
     4441 ggctggcaaa gatgattgat tctttaaagt ttttcaatcc atcattcaag cttgagatag
     4501 attccaagtc caagtgattt gttggatcat caagtacaag gacatttgat tttaagagca
     4561 tgagttttga aagcatgaca cgaacttttt ctccccctga caagacattt acaggtttgt
     4621 taacttcatc tccagagaag agcatacggc cgaggaagcc acgtaggaaa gtattgtcat
     4681 cttcttcttt acttgcgaat tgacgcaacc agtcaagaat tgattctcct cctgcaaaat
     4741 cagctgagtt atcttttggt aggtaagatt gactagttgt aactccccac ttgacagttc
     4801 cttcatagtc aatatctccc atgattgcac gaattaatgc agtcgtttga atatcatttt
     4861 gtccaataag tgctgtctta tcatctggac gcaagatgaa actaatatta tccaagatag
     4921 tttcaccatc aatctttaca gttagatttt ctactgtcaa gagatcatta ccaatctcac
     4981 gttccgcttt aaagttgata aatggatatt tacgactaga tggtacaatc tcttctagct
     5041 caatcttatc aagcattttc ttacgtgatg tggcctgcct tgacttagaa gcattggcag
     5101 agaaacgagc aacgaattct tgcaattgtt taattttttc ttctgcttta gcattacggt
     5161 ctgctagcaa tttagcagca agctcagaag attctttcca gaagtcgtag tttccgacat
     5221 agagtttgat ttttccaaag tcaaggtcgg ccatgtgagt acaaactttg tttaagaagt
     5281 gacggtcgtg ggatactacg ataactgtgt tatcaaagtc aatcaagaag tcttctaacc
     5341 aagtaatcga ttggatatcc aaaccgttag taggctcgtc caagagaaga acatctggtt
     5401 taccaaaaag tgctttggcg aggagaacct ttactttttc accgttggcc aattcgctta
     5461 tgttttggta gtgtaattct tctggaatgt ttaggttttg aagtagttga gaggcttcac
     5521 tctctgcttc ccaacctcca agttcggcaa actctccttc gagttcggca gcacgaaccc
     5581 cgtcctcgtc tgagaaatct tccttcatgt agatagcatc tttctctttc atgatgctat
     5641 aaagtttttc atttcccatg ataacgacat caatggcacg ttcatcttcg tagtcaaagt
     5701 gattttgacg aagaacagag agacgttcat ctggaccaag agagatgtga ccagtagtag
     5761 gttcgatatc tccagctaaa atttttaaaa aggttgattt tccggcacca ttagcaccga
     5821 ttaatccgta agtatttcct tctgtaaatt tgatattgac atcatcaaaa agtttgcgat
     5881 cactaaaacg tagtgaaaca tcagatactg taagcaatat ttttctccta tatgtgtaat
     5941 atatttattc ta
//
LOCUS       DAVKZB010000100         5933 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKZB010000100 DAVKZB010000000
VERSION     DAVKZB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.100"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..177
                     /locus_tag="VXA80_001667"
     CDS             <1..177
                     /locus_tag="VXA80_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0354128.1"
                     /translation="DKEVEVSLLFFIITNSSSSQTNILAQLMNFFTGNGHLEDLSKIS
                     EPEKLYAYIDEAIA"
     gene            215..505
                     /locus_tag="VXA80_001668"
     CDS             215..505
                     /locus_tag="VXA80_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0354129.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAK"
     gene            525..1327
                     /locus_tag="VXA80_001669"
                     /pseudo
     CDS             525..1327
                     /locus_tag="VXA80_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1376..1840
                     /locus_tag="VXA80_001670"
     CDS             1376..1840
                     /locus_tag="VXA80_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV0354130.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1997..2422
                     /gene="lacA"
                     /locus_tag="VXA80_001671"
     CDS             1997..2422
                     /gene="lacA"
                     /locus_tag="VXA80_001671"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV0354131.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2453..2968
                     /gene="lacB"
                     /locus_tag="VXA80_001672"
     CDS             2453..2968
                     /gene="lacB"
                     /locus_tag="VXA80_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV0354132.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2979..3908
                     /locus_tag="VXA80_001673"
     CDS             2979..3908
                     /locus_tag="VXA80_001673"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV0354133.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            3910..4890
                     /gene="lacD"
                     /locus_tag="VXA80_001674"
     CDS             3910..4890
                     /gene="lacD"
                     /locus_tag="VXA80_001674"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV0354134.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5124..>5933
                     /locus_tag="VXA80_001675"
     CDS             5124..>5933
                     /locus_tag="VXA80_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0354135.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLV"
BASE COUNT         1952 a         1032 c         1247 g         1702 t
ORIGIN      
        1 gataaggaag tagaagtatc cctactcttc tttatcatta ctaattcaag ttcaagtcaa
       61 acgaacattc ttgctcagtt gatgaacttc ttcacaggaa atggacatct tgaagaccta
      121 tcaaaaattt ccgaaccaga aaaactttat gcttatatag atgaagcaat cgcttaatct
      181 tgtttattaa aaaataaaat cggaggaagt ctatatgatt aaaattcttg ctgcctgcgg
      241 tgcaggtgtt aactcaagtc accaaattaa aagtgctcta gaagaagaac tttcaaaccg
      301 tggttacgat gttcactgtg atgcagttat ggtgaaagat gtaaacgaag accttatgaa
      361 aggttacgat atctttacac caatcgctgc aacagacctt ggttttgaac caggtatccc
      421 agttatcgaa gctgggccaa tcttattctg tatcccagca atgagcgttc cagtatttga
      481 caatattaga cttcctgcga aataaaatat ggtatagtag ttctatgaat gatgaagcaa
      541 gtaaacaact aactgatgca cgatttaagc gtcttgttgg tgttcagcgc acgacttttg
      601 aagagatgtt agctgtatta aaaacagctt atcaacttaa acacgcaaaa ggtggacgaa
      661 aacctaaatt aagcctagaa gaccttctta tggccactct tcaatatgtg cgagaatatc
      721 gaacttatga agaaattgcg gctgattttg gtatccacga aagcaactta ctccgtcgga
      781 gctaatgggt tgaagtaact cttgttcaaa gtggttttac gatttcaaga actcctctca
      841 gttctgagga cacggtaatg attgatgcga cggaagtaca aatcaatcgc cctaaaaaag
      901 aattagcgaa tgattctggt aaaaagaaat gccacgctat gaaggctcaa gcgattgtca
      961 caagtcaagg gagattgttt ctttggatat cactgtgaac tattgtcatg atatgaagtt
     1021 gttcaaaatg agtcgcagaa atatcggaca agctggtaaa atcttggctg acagtggtta
     1081 tcaagggctc atgaagatat atcctcaagt acaaactcca cgtaaatcca gcaaactcaa
     1141 gccgctaaca gttgaagata aaacctataa ccatgcgcta tctaaggaga gaagcaaggt
     1201 tgagaacatc tttgccaaag taaaaacgtt taaaatgatt tcaacaacct atcgaaatca
     1261 tcgtaaacgc ttcggattac gaatgaattt gattgctggc attatcaatc atgaactagg
     1321 attctagttt tgcaggaagt ctaataaaga aactagcaag gtcaggcctt actttattgg
     1381 tggacatcca ggatttaatt gtcctctact tgatgatgaa gtctatgaag attattatct
     1441 agagtttgag aaagaagaga cttgctctgt tccacgtcct ttcccagaaa caggtatgtt
     1501 ggatttccaa gatagaagtc catggctaga gggacaaaaa gaaatagatc ttagttatga
     1561 tcttttcagc aaagatgcag tgactttgga tgaattgcaa tctcgaacaa ttgcccttcg
     1621 ttctcttaaa catgataggg gattgaaagt gcattttgca gaatttccaa acctcatcat
     1681 ctggtcaact ttgaacaagg gacctttcat tacctttgaa ccatggtctg gcttgtcaac
     1741 attccttgaa gaaggagatc atttagaaga taagaagaat gtttgtctct tagaagccaa
     1801 tcaggttgaa gaattagggt ttgagataga agttttataa aaaagtaaaa aataaataac
     1861 aaaaacaaac acaaactgtt gacttcttca aacaatagta gtatattatg tttgtgagga
     1921 ctaattgact gttttttaaa gacagccaat acactgctgg aatccagcat agaaaaaata
     1981 aaaaggagta tacaatatgt ctattgttat cggtgcagat gctgcaggtt tgagattgaa
     2041 agaagttgtg aaagacttct tagaaaaaga aaacttccac cttgtggatg ttacagctga
     2101 aggtcaagac tttgttgatg tgactctcgc tgttgctgca gaagtaaaca aagaagaaca
     2161 aaaccttggt atcgtgattg atgcttatgg agctggtcca tttatggttg caactaagat
     2221 caaaggaatg gttgctgcag aagtttctga tgaacgttca gcttatatga ctcgtggcca
     2281 caacaactca cgtatgatca ctatgggtgc acaacttgtt ggtgatgaat tggctaaaaa
     2341 catcgctaaa ggatttgtta acggtaaata cgatggtgga cgtcatcaaa tccgggtcga
     2401 catgttgaac aaaatgggct aattagatta cagtaagaga ggtaagttaa aaatgagaat
     2461 tgcaattgga tgcgaccaca tcgtaacaga tgaaaaaatg gcggtttcag aatttttgaa
     2521 atcaaaagga tatgaagtca ttgactttgg tacatatgac catacacgga ctcactaccc
     2581 aatctttggt aaaaaagtag gggaagctgt aactagcggc caagctgatc ttggggtatg
     2641 tatctgtggt actggtgttg gtatcaacaa cgctgtaaat aaagttccag gtgttcgttc
     2701 tgccttggtt cgtgatatga caacagccct ttatgctaaa gaacaattga acgctaacgt
     2761 tatcggcttt ggtggtaaga ttactggtga attgcttatg tgtgatatca tcgaagcttt
     2821 cattcatgct gaatacaaac catctgaaga aaacaagaaa ttgattgcga aaattgaaca
     2881 ccttgaaagt cacaatgctc aacaaacaga cgcaaacttc tttacagaat tccttgaaaa
     2941 atgggatcgc ggagaatacc acgactaaga ggtgacctat gattttaaca gtcacaatga
     3001 acccatccat tgatatttcc tatcccttgg atgagttgaa gattgatact gtcaatcgtg
     3061 tggtggatgt aaccaaaacg gctggtggta agggactcaa tgttacccga gtactttcag
     3121 aatttggcga ttctgttctt gctactggtt tagtgggtgg caaacttggt gagtttttgg
     3181 ttgaacatat cgataatcaa gtaaagaaag atttcttctc aattcaggga gaaactcgta
     3241 actgtatcgc tattctccac ggagacaacc aaacagaagt tcttgaaaaa ggtcctgaag
     3301 tattggaaca ggaaggtcaa gactttttgg aacatttcaa aaaactcttg gagtcagttg
     3361 aagtagtggc catctcaggt agtctgccag ctggccttcc agttgattac tatgcgagct
     3421 tggtagaact tgctaatcaa gctggcaagc ctgtagtctt ggactgctca ggtgcagcac
     3481 ttcaggctgt tcttgaatca ccccataaac caacagtcat caaaccaaat aatgaagaat
     3541 tgtctcagct tcttggaaga gaagtttctg aggatttgga tgaattaaaa gaagtacttc
     3601 aagaaccttt gtttgcaggg attgaatgga ttatcgtttc acttggtgcc aacggtactt
     3661 ttgccaaaca tggtgacact ttctacaagg tagatattcc tagaattcag gtggtaaatc
     3721 ctgttggatc tggagactct actgtggcag gaatttcttc aggacttctt cacaaagaat
     3781 cggatgcaga attactcatc aaggcaaatg tccttggtat gctcaatgct caagaaaaaa
     3841 tgactggtca tgtcaacatg gccaactatc aagctctata tgatcaatta atagtaaaag
     3901 aggtataaaa tggctttaac agaacaaaaa cgtgtacgct tagaaaaact ttctgatgaa
     3961 aatggtatca tctcagctct tgcatttgac caacgtggtg ctttgaaacg cctcatggct
     4021 caacaccaaa cagaagaacc aactgtggcc caaatggaag aacttaaagt cttggtagca
     4081 gatgaattga ctaaatacgc ttcatctatg cttcttgacc ctgagtatgg acttccagca
     4141 actaaagctc ttgatgaaaa agctggtctt ctccttgctt atgaaaaaac aggttatgac
     4201 acaacaagca caaaacgctt gccagactgc ttggatgttt ggtctgcaaa acgtattaaa
     4261 gaagaaggtg cagatgcagt taaattcttg ctttactatg atgtagatag ctcagacgaa
     4321 ctcaatcaag aaaaacaagc ctacatcgaa cgcatcggtt ctgagtgtgt ggctgaagat
     4381 atcccattct tccttgaaat ccttgcttac gatgaaaaaa ttgcggatgc aggttctgta
     4441 gaatacgcga aagtaaaacc acacaaagtt atcggcgcta tgaaagtctt ttcagaccca
     4501 cgctttaaca ttgatgtctt gaaagttgaa gttcctgtta acattaaata tgttgaaggc
     4561 ttcgctgaag gtgaagtagt ttatacacgt gaagaagcag cagccttctt caaagcgcaa
     4621 gatgaagcaa cgaacttgcc atacatctac ttgagtgctg gtgtatcagc taaactcttc
     4681 caagatactc ttgtatttgc tcatgaatca ggtgcaaact ttaacggagt tctttgtggc
     4741 cgtgctacat gggcagggtc agttgaagct tacatcaaag atggtgaagc agcagctcgc
     4801 gaatggcttc gcacaactgg atttgaaaac attgatgagc tcaataaagt tcttcaaaca
     4861 acagcgactt catggaaaga acgtgtgtaa gaaagtcctc ctagtttagg aacatgaatc
     4921 taaaaaaatt taaaaaaagt tgtatgtaaa ggtttacaaa acaaccgact tgtgctatac
     4981 ttaaatcaca agttaataca aggtgagtgt tactaagtaa tattaggcat gatcataggt
     5041 gaattagaaa tcagctgatt ttctagttca tttgtggtca ttttttgtac tcatatacct
     5101 ttaagatata aaaggaggtt gacatgtatc gaattctaaa tccaatgaat cacaatgtct
     5161 cgcttgtcag aaatgataag ggagaagagg tgattataat tggtaagggg attgcattcg
     5221 gaaaaaggaa gggggatttg attgctgaaa atcaggttga gaaaatcttt cggatgaaga
     5281 ccgaagagtc cagagaaaac tttatggctc ttctcaaaga tgttccgctt gattttatca
     5341 cagtgaccta tgaaatcatt gataagctat caaagaaata tcattatccg attcaagagt
     5401 atctctatgt aaccttgaca gatcatattt actgttctta tcaagctcta actcaaggaa
     5461 ggtacaagga tagtaatctg ccggatattt ccgctaagta tcctgtcgct tttcaaatcg
     5521 caaatgaagc ttttgaaatt taccgtcaga agctagcaga tcattttcct gaggacgaga
     5581 ttattcggat tgcttatcat ttcatcaatg ctgaaggtga aaatgaagtg gaacttgtgg
     5641 agtcgattga taagaggaaa gaaattctca ggaatgttga agaagtgcta aagggttatg
     5701 caattcaacg aaccaaaaaa aataatcatt tctatgatcg ctttatgatt catttgaatt
     5761 atttcttgga ttatttagac agatctagag atgataacca atcacttctg gatatggaag
     5821 atcatattaa acaatcctat ccaaaagcct tcgagattgg ttccaagatc tatgatgtga
     5881 ttacgcaaca tacgggtctt gatttgtata aaagtgaacg agtttatcta gtt
//
LOCUS       DAVKZB010000101         5795 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKZB010000101 DAVKZB010000000
VERSION     DAVKZB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.101"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            62..1282
                     /locus_tag="VXA80_001676"
     CDS             62..1282
                     /locus_tag="VXA80_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV0354136.1"
                     /translation="MTNSVSSNRPEKYNIAAFFSGVGGIELGFEQTNEFRVVYANEFD
                     KYARQTYQLNHPDTYLDGRDIHDVQPEDIPAERVDVIMGGFPCQAFSIAGYRKGFDDD
                     RGDLFFELLRMIEGRKPRAIFIENVKNMVGHDHGNTFKVIREALTENNYFIKWKVLNG
                     KDYGNIPQNRERIYIVGFNTKEAYDLFEFPEEIKLTTSLQDVINFGDQLGEVYYYRDG
                     KQNFYDQLKFEVTSQDTIYQWRRQYVRENKNGVVPTLTANMGTGGHNVPLILTDSGEI
                     RKLTPKETFNVQGYPKFFKIPEGVSNGQLYKQAGNSVVVPVIKRIAENVAKALNESQG
                     QSQLNRSGKFAIIYTKMNGQFEGQSYVKDFVDSYDQALEKIKSYDDGLAVLSGEDYLK
                     LVKKRGTLEFYSIN"
     gene            1353..2930
                     /locus_tag="VXA80_001677"
     CDS             1353..2930
                     /locus_tag="VXA80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354137.1"
                     /translation="MWKHLKSEQKDKYKTLITNFASLSQAFSQKAESDDEGQTENYVA
                     PIVNSKFQETVFQKAFNAVGEDIANTSYDASVVVDENHKYLVGIKSFGINSGDQKIAQ
                     FKKDSQDWTELLGDIKFHADISVDKETADKKNYQRYEELARKIATLRNQRIESSKAQI
                     KGFSSDSVNVEAVYHVLMPTPKGENPKIFVGETSYLPVDIDNLIIEGSTTKNNPTNFR
                     FTDGQHHYKYTAADSQLHMTFNNKDIVVDTWDVHYIEDPFSLFENLHLLTAEKDKTDV
                     LETVSWVITDKHGNVEENSGFNAFNGGSKLAKKDRLPRILKTQEKFKDSLAPEELAFV
                     TFSLEEILLKKWTSKEEKVQMKAIREDLIHFVHNTGNEKLIKEIEQLVYRPVSEVYIP
                     LPDSKSFHDERPDFFGPGFGTFEPGSKKLAMAKEERTFKLRFLPSGDVINAYINQEAG
                     KAIQSTDKQEILGNWILRGVFQLKEREVLTGQRLNELEINGIRLTKFKNGEIGIEFIW
                     IDTENPPSDAIGWVAKK"
     gene            3033..5712
                     /locus_tag="VXA80_001678"
                     /pseudo
     CDS             3033..5712
                     /locus_tag="VXA80_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1818 a          943 c         1306 g         1728 t
ORIGIN      
        1 tttaaaaaaa tataaatagt ttatagaaat agttgtttaa ttttagaaag caggttacta
       61 catgacaaat tctgtaagtt cgaaccgacc ggagaagtac aacattgcag ctttcttctc
      121 aggtgttggg ggaattgagt taggttttga acagaccaac gagttcagag tggtgtatgc
      181 caatgaattt gataagtatg cgcgtcagac ttatcagtta aaccatccag atacctattt
      241 ggatggtcgt gatattcacg atgttcagcc agaggacatt ccagctgagc gcgtggatgt
      301 gattatggga ggtttccctt gtcaggcctt tagtattgcg ggttatcgca agggttttga
      361 tgacgatcgt ggcgatcttt tcttcgaatt gcttcgcatg attgaagggc gtaaacctcg
      421 cgctattttc attgaaaacg tcaaaaacat ggtaggtcat gaccacggca acacctttaa
      481 ggtcattcgt gaagccttga ccgagaacaa ctattttatc aagtggaagg ttcttaacgg
      541 aaaagactat ggaaatatcc cacaaaaccg tgagcggatt tatatcgttg gttttaatac
      601 caaggaagct tatgatttgt ttgaatttcc agaggaaatt aagctgacta cgagtcttca
      661 ggatgtgatt aacttcggcg accagctagg cgaggtttac tactatcgtg atggcaagca
      721 gaatttctac gaccagttaa aatttgaagt cactagccaa gatacgattt accagtggcg
      781 ccgtcagtat gtccgtgaaa ataaaaacgg tgttgttcca accctaacag ccaatatggg
      841 aacaggggga cacaatgtcc ctctgatctt gacagacagt ggcgagattc gtaaactgac
      901 acctaaggaa acctttaacg ttcaaggcta tcctaagttc tttaaaattc cagaaggggt
      961 ttccaatggt cagctctaca aacaagctgg aaatagcgta gttgttcctg tgattaaacg
     1021 tatcgcggaa aatgttgcta aggctttgaa cgaaagccaa ggtcaatctc aacttaaccg
     1081 ctcaggaaaa ttcgctatta tctacaccaa gatgaatgga caatttgaag ggcaatccta
     1141 tgtcaaggac tttgtagata gttatgatca agctcttgaa aaaatcaaat cttatgacga
     1201 tggtttagct gttctgtctg gtgaagatta tttaaaattg gttaaaaaac gaggaactct
     1261 tgaattttac agtattaatt aacgaatata taagagggtt ggtgaaaaat taatctcctt
     1321 ttgatatact gaaaatagga aagaaggtgc ttatgtggaa acatctaaag tctgaacaaa
     1381 aagacaaata caaaacctta attaccaact tcgctagctt gagccaagct ttctctcaaa
     1441 aggctgagtc cgacgatgag ggacaaacag agaattatgt tgctccgatt gttaattcta
     1501 aatttcaaga gactgttttt cagaaggctt ttaatgctgt tggggaagat attgccaata
     1561 cgtcctatga tgcctctgtc gttgtggatg aaaatcataa atacctagtg ggtatcaagt
     1621 cttttggaat caattctgga gaccaaaaaa ttgcccagtt taagaaggac tctcaagatt
     1681 ggacggaatt gttgggtgat atcaaattcc atgcagatat ctcagtagat aaagaaacag
     1741 cagacaagaa aaactatcaa cgctatgaag agttggcacg aaagattgcg accttaagaa
     1801 atcagagaat cgaatcttct aaggcacaaa tcaagggatt tagttcggac tcggtcaatg
     1861 ttgaagctgt ttatcacgtt ctgatgccga caccgaaggg agaaaatcct aagatttttg
     1921 tcggtgaaac atcttatttg ccagttgata ttgataatct gatcattgaa ggatcaacta
     1981 ccaaaaacaa tccaaccaat tttcgattta cggatgggca acaccactac aaatacacag
     2041 cggcagatag ccagctccac atgactttta acaataaaga cattgtggta gatacttggg
     2101 acgtccatta tattgaagat cctttttcgc tctttgaaaa ccttcattta ctgacagctg
     2161 aaaaggataa aaccgatgtc ttagaaacag tgtcttgggt tattacagat aaacatggaa
     2221 atgtggaaga aaactctggt ttcaatgcct ttaacggagg atcaaaacta gctaagaaag
     2281 atcgtctgcc acggattcta aagactcagg aaaaattcaa agacagtctg gctccagaag
     2341 aactagcctt tgtgactttt tctttagaag aaatcctctt gaaaaagtgg actagcaagg
     2401 aagaaaaagt tcagatgaag gctattcgtg aggacttgat tcactttgtc cacaatactg
     2461 gcaatgaaaa actcatcaaa gagattgaac aactagttta tcgtccagtt agtgaagtct
     2521 acattcctct gccagactct aagagcttcc atgatgaaag accggatttc tttggtcctg
     2581 gatttgggac ttttgaacct ggctctaaaa aactagctat ggcaaaagaa gaaagaacgt
     2641 ttaaactacg ctttttacct tctggagatg tcatcaatgc gtatataaat caagaagctg
     2701 gaaaggcgat tcagtcaact gataagcaag aaattcttgg gaattggatt ttacgtggtg
     2761 ttttccagtt gaaagaacga gaagtcttga caggtcaacg actcaatgaa ctggaaatca
     2821 acggtattcg tttaactaaa tttaaaaatg gggaaatcgg tattgaattt atctggattg
     2881 ataccgaaaa tcccccaagc gatgccatcg gctgggtagc aaagaaataa aagttttcaa
     2941 aggtattgta acaaataagg gattttattt tcgtgtacaa tttcagctat tcttgttata
     3001 atcagcctat aggaatcaag gaggtgactc ttatggctgt ttttgtgtct ttggatggaa
     3061 ttgtggtaga agtgcttgat gtcttttctt cttttgatgg ggatagtgag tttttcttgt
     3121 gtaaacgtct taaagacaag agtcagtttg ttatggaacg ctctcagttc gaggagatgt
     3181 tccaacttca aagtagtcgc ttgacgacgc aagaaaaatt acaattgttt acctctgtgt
     3241 ttgctggccg ttatgatgtt tatgctaaga gttttatcaa tgatcaaggg aaaattcagt
     3301 attttccttc ctatgattat ggttggaagc agttgctacc tgaaaaacgg agtttccaga
     3361 cattgacgga ttccgttttg aaatctcatt ttcgtgggga gacagctatc ggtatctttc
     3421 ctatgcactt agatgatagc tgtcattttt tggtactgga ttttgatgaa ggagattgga
     3481 aagaagctgg tttaaccatt cgaagaatag ccagggaacg ccagatggaa gcccatttag
     3541 agatttctcg ttcgggtcac ggactccata tttggttctt ctttgaggaa gcgattccga
     3601 gtcgagaggc tcgcttgttt ggaaagaaac tgatagaact ggcaatgcag gaaagtatgc
     3661 aactgtcctt tgattctttt gatcgcatgt ttccaaatca ggatgtcctt cctaaggggg
     3721 gatttggaaa tttgattgcc ttgccttttc aaggagaagc ttaccatcaa gggcgaacgg
     3781 tctttgtgga tgaacagttt cagccttatg aagaccaatg gaggtatcta caagaaattc
     3841 agagggtttc aactgctaaa gtggcactgt taatccaaga agagttaggc aagcaagaat
     3901 tggacaaggg gttgaagatc gtcttatcca atatgatcca acttgaaaaa tcgtctgtga
     3961 cacccaagac actgtttttc ttgaaaaata tggcttcatt ttctaatccc gaattttatt
     4021 taaagcaggc tatgcgacag ccaacctatc aaattcctga gcgaatgtat ttatttggag
     4081 aatccgatta ttatttatgg ctgccaagag gcttgttata tccattgcaa gataaattta
     4141 agcaggtagt tgtggaggat aggagaaagg tacaaaggtc tattagagta gcatttaagg
     4201 gagagcttac tttggagcaa gaattagccc tgtcagatat gaattctaaa gaaaatggtt
     4261 tacttcatgc gggacaggtt ttggaaagag cgttttaggt gctgctctta tctctgaacg
     4321 taagacaaaa accattatcc tagttcataa taggcaactc ttagaccaat gactggatcg
     4381 tttaaagtgt tttttgactt tcgaagaggc agaagctacc cgttatacac catcaggtcg
     4441 tgaaagggta ataggctatg tcgggcagta cggtggggct aagaaatgac tgagtaaact
     4501 ggttgatgtt gttatgattc aatctctatt taagttggaa aatagtcaaa gtcttttgga
     4561 tgagtatgag atgatgattg tggatgagtg tcatcatatc tctgccttga tgtttgaaaa
     4621 agttgttgct cagtttagag ggaagtatct ttacggtttg acggctacgc ctgagcgtaa
     4681 gaatggtcat gagcctattg tttttcagag aattggtgag atactccata ctgctgataa
     4741 gagggaaacg gattttaaac ggcaattgca attaagattc acttcttttg gtcatttgga
     4801 gattgaaaag accaaagcaa gtaattttat acagcttagt gattggattg ctactgactc
     4861 agcgaggaat cagttgattc tcaaggatat tctagcccaa gtggcagaag gacggaatat
     4921 cttggtttta gttaatcgaa ttcaacagat agatgtcttt gaaaagttat tgaaagagaa
     4981 agaggttgat gactgttaca ttattagcgg aaaaaccaac gtccgagaaa gagaacgagt
     5041 ttactggaga cgttagaaca gttagataaa gggtttattt tgttgtttac tggaaaatac
     5101 attggcgaag gttttgactt acctcagttg gacacgctta tcttggcagc acccttttct
     5161 tggaaaaata atttgattca gtacgcaggt cggattcata gaaactacaa ggataagtct
     5221 ttggtgcgta ttttcgatta tgtggatatt catgttcctt atttagaaaa gatgtttcag
     5281 aaacgacaag tagcttatcg aaagatggat tatcgtgcca ttgagggtga ggagaaacaa
     5341 ttcgtttatg ttgatagtag atatgagaag gtgttgagag aggacttagc aggggaaaga
     5401 caggaatgta tgcttatttt accttatgtg caccagacaa aactgatgaa ttttctaaaa
     5461 gaatttagga ttagtcaaat tgagatatgt ataccagaga cggttgcaaa taaagcatgg
     5521 ctagaccagt tgaagagcca gaaaattaaa gtgtctttta ctcaatcaaa aatagtaacg
     5581 cctattcttt tggtgaataa gactattgtt tggtatggtg caatgccatt attagggaag
     5641 gtagatgaga tgaccatatt acgtttggaa tcagctagta tagtttctga actagtggca
     5701 ggtttacgat agagaaaatt tttaaaaatt tctatgtatg attttcattt ctttagtgag
     5761 actgttgcca ttatcacatt cgaatcacac aaaat
//
LOCUS       DAVKZB010000102         5687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKZB010000102 DAVKZB010000000
VERSION     DAVKZB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.102"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            178..1623
                     /gene="cps4A"
                     /locus_tag="VXA80_001679"
     CDS             178..1623
                     /gene="cps4A"
                     /locus_tag="VXA80_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV0354138.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIKKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGDYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1625..2356
                     /gene="cps4B"
                     /locus_tag="VXA80_001680"
     CDS             1625..2356
                     /gene="cps4B"
                     /locus_tag="VXA80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV0354139.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2365..3057
                     /locus_tag="VXA80_001681"
     CDS             2365..3057
                     /locus_tag="VXA80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEV0354140.1"
                     /translation="MKEQNTIEIDVFQLFKTLWQRKLMILLVALVTSAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVAVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKMK"
     gene            3067..3756
                     /locus_tag="VXA80_001682"
     CDS             3067..3756
                     /locus_tag="VXA80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV0354141.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     LGEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSIDKYGSY
                     GNYGNYGKNKK"
     gene            3771..5138
                     /locus_tag="VXA80_001683"
     CDS             3771..5138
                     /locus_tag="VXA80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0354142.1"
                     /translation="MNGKIVKSSLVIIQSSLVILLTYLLSTVREAKIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDGLIESNEVVGEL
                     VAVSVLDKPDFQHDYLKIVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIRKMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGCFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            5145..>5687
                     /locus_tag="VXA80_001684"
     CDS             5145..>5687
                     /locus_tag="VXA80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0354143.1"
                     /translation="MFMKLLHFSEVGGGVDRYIKLYLKYSDKEHFKNIVVGSDQLNRQ
                     TYEQEYNIKFYHIDIYRSLSPIKLLRAIKQFRKILYLERPDIVYLHSTFAGVVGRLAS
                     MGLSCKVVYNPHGWSFKMDVSKIKQFVYKNIEKFLSYLTDKYILISKSEYEAAQSLKI
                     PLKKLTLVYNGIEIDEDFNEN"
BASE COUNT         1790 a          914 c         1243 g         1740 t
ORIGIN      
        1 ttagaaatcg atttgactgt cctgatcgtt ttgtcatgtt cttatttcat tttactatat
       61 ttttggttcg tgggaagtct actaagatac ttaaagatgc agatagtgaa aaaaggtgta
      121 gacattaccg taaaaaagtg atataatcgt atgatgttca atgtataggt gttaatcatg
      181 agtagacgtt ttaaaaaatc aggttcacag aaagtgaagc gaagtgttaa tatagttttg
      241 ttgactattt atttattgtt agtttgtttt ttattgttct taatctttaa gtacaatatc
      301 cttgctttta gatatcttaa tctagtggta actgcgttag tcctactagt tgccttggta
      361 gggctactct tgattatcta taaaaaagct gaaaaattta ctatttttct gttggtgttt
      421 tctatccttg tcagctcagt gtcgctcttt gcagtacagc agtttgttgg actgaccaat
      481 cgtttaaatg cgacttctaa ttactcagaa tattcaatca gtgtcgctgt tttagcagat
      541 agtgagatcg aaaatgttac gcaactgacg agtgtgacag caccgactgg gactgataat
      601 gaaaatattc aaaaactact agctgatatc aagtcaagtc agaataccga tttgacggtc
      661 gaccagagtt cgtcttactt ggcagcttac aagagtttga ttgcagggga gactaaggcc
      721 attgtcttaa atagtgtctt tgaaaatatc atcgagtcag agtatccaga ctatgcatcg
      781 aagataaaaa agatttatac caagggattc actaaaaaag tagaagctcc taagacgtct
      841 aagaatcagt ctttcaatat ctatgttagt ggaattgaca cctatggtcc tattagttcg
      901 gtgtcgcgat cagatgtcaa tatcctgatg actgtcaatc gagataccaa gaaaatcctc
      961 ttgaccacaa caccacgtga tgcctatgta ccaatcgcag atggtggaaa taatcaaaaa
     1021 gataaattaa cccatgcggg catttatgga gttgattcgt ccattcacac cttagaaaat
     1081 ctctatggag tggatatcaa ttactatgtg cgattgaact tcacttcgtt tttgaaattg
     1141 attgatttgt tgggtggaat tgatgtttat aatgatcaag aatttactgc ccatacgaat
     1201 ggaaagtatt accctgtagg caatgttcat cttgattcag aacaggctct cggttttgtt
     1261 cgtgagcgct actcactagc agatggcgat cgtgaccgtg gtcgcaacca acaaaaggtc
     1321 attgtagcaa ttattaagaa gttaacttct acagaggttt tgaaaaacta tagtagtatt
     1381 cttcaaggat tgcaggattc tcttcaaaca aatatgccga ttgagactat gatagattta
     1441 gtgaatactc agttggaaag tgggggggat tataaagtaa attctcaaga tttaaaaggg
     1501 acaggtcgga tggatcttcc ttcttatgca atgccagaca gtaacctcta tgtgatggaa
     1561 atagatgata gtagtttagc tgtagttaaa gcagctatac aggatgtgat ggagggtaga
     1621 tgaaatgata gacatccatt cgcatatcgt ttttgatgta gatgacggtc ccaagtcaag
     1681 agaggaaagc aaggctctct tggcagaagc ctacagacag ggggtgcgaa ccattgtctc
     1741 tacctctcac cgtcgcaagg gcatgtttga aactccggaa gagaagatag cagaaaactt
     1801 tcttcaggtt cgggaaatag ctaaggaagt ggcgagtgac ttagtcattg cttacggggc
     1861 tgaaatttac tacacaccag atgttctgga taagctggga aaaaagcgga ttccgaccct
     1921 caatgatagt cgttatgcct tgatagagtt tagtatgaac actccttatc gcgatattca
     1981 tagcgccttg agcaagatct tgatgttggg aattactcca gtcattgccc acattgagcg
     2041 ctatgatgct cttgaaaata atgaaaaacg cgttcgagaa ctaatcgata tgggctgtta
     2101 cacgcaagta aatagttcac atgtcctcaa acccaaactt tttggcgaac gttataaatt
     2161 catgaaaaaa agagctcagt attttttaga gcaggatttg gtccatgtca ttgcaagtga
     2221 tatgcacaat ctagacggta gacctcctca tatggcagaa gcatatgacc ttgttaccca
     2281 aaaatacgga gaagcgaagg ctcaggaact ttttatagac aatcctcgaa aaattgtaat
     2341 ggatcaacta atttaggaga aatgatgaaa gaacaaaaca cgatagaaat cgatgtattt
     2401 caattattta aaaccttgtg gcaacgcaag ctaatgattt tattagtggc acttgtgaca
     2461 agtgcggggg cttttgcata tagcactttt attgttaagc cagaatatac gagtaccacg
     2521 cgaatttacg tagtgaatcg caatcaagga gacaagccgg ggctgacaaa tcaggatttg
     2581 caggcaggaa cttatctggt gaaagactac cgtgagatta tcctttcgca ggatgcattg
     2641 gaaaaagtag cgacaaattt gaagttggat atgccagcaa aaacgttagc cagcaaagtt
     2701 caagtggctg taccagctga cactcgtatc gtctcaatct ctgtcaagga taaacagcca
     2761 gaggaagcca gtcgtatcgc taattctcta cgagaagttg ctgcagaaaa gatcgtcgct
     2821 gtaacgcgag tatctgatgt aacgacactt gaagaagcgc gaccagctac aactccctct
     2881 tctccaaatg ttcgacgcaa ttccttgttt ggttttcttg gaggagcagt cgtaacagta
     2941 attgctgttc ttttgattga gttgctcgac acccgtgtga aacgtcctga agatgttgaa
     3001 gatgtactga aaattccact tttagggctc gttccagatt ttgacaaaat gaaataggag
     3061 gaagttatgc caacattaga aatctcacag gcaaaattgg attctgtaaa aaaggcagag
     3121 gaatattata acgctttgtg cacgaaccta cagttaagtg gagatggttt gaaagtattt
     3181 tctatcactt ctgtgaaact aggagaagga aaatcaacga cttccaccaa tatcgcttgg
     3241 gcttttgcgc gtgcaggata caaaacgcta ctgattgatg gagatattcg caattctgtt
     3301 atgttaggtg tctttaaagc aagggataag attacaggcc tgacagaatt tttatcagga
     3361 actacagacc tatcacaagg gctttgtgat accaatatcg aaaatctatt tgtaattcag
     3421 gctggctctg tgtcaccgaa tccgacagct cttcttcaaa gtaagaattt cactacaatg
     3481 cttgaaacct tgcgtaaata ttttgactac atcattgtag atactgctcc tgtcggtgtc
     3541 gtgattgatg cggctattat tacgcgaaaa tgcgatgctt ctattttagt gacggaggca
     3601 ggtgaaataa atcgacggga tattcaaaaa gcaaaagaac agttggaaca cacagggaag
     3661 ccgtttttgg gagttgtgtt gaataaattc gatacttcaa tagacaaata cggttcttat
     3721 ggaaattatg gaaattacgg gaaaaataaa aaataggtcg ggggatagag atgaatggaa
     3781 aaatagtaaa gtcttcattg gtcataatcc agagttctct tgttatttta ttgacttatc
     3841 tacttagtac tgtgagagaa gcgaagattg tttcaacaac agctattgca ctttatatcc
     3901 tccattattt tgtcttttat atcagtgatt atggacagga tttctttaaa aggggatatt
     3961 tgattgaact tgtccagaca ttgaaatata tcctattctt tgcactagcg attagtattt
     4021 ctaatttttt cttagaggat cgatttagta tttccagacg aggcatgatt tacttcctca
     4081 cattacatgc tctcttagtc tatgtgctaa acctatttat caagtggtat tggaagcggg
     4141 cttatcccaa ctttaaagga agtaagaaga ttctcctact tacagcaact tctcgtgtcg
     4201 aaaaggtact ggatggatta atagaatcaa atgaggttgt tggggagttg gtagccgtca
     4261 gtgtcttaga taaaccagat tttcagcatg attatttaaa gatagtagca gagggggaga
     4321 tagtaaactt tgcgactcat gaggtggtcg atgaagtctt tatcaatctt ccaagtgaaa
     4381 aatacaatat tggagagctt gtctctcagt ttgaaacgat gggaattgat gtaacagtca
     4441 atctaaatgc ttttgatcgt agtttggcac gtaacaagca aattcgtaag atggcaggat
     4501 taaacgttgt gactttttct acaacatttt ataagactag tcatgtaatt gctaagcgga
     4561 ttattgatat cgtgggtgca ttggtcgggt tgatattatg tggtttagtc agtattgtac
     4621 tggttccttt gattcgaaag gatgggggct ctgctatttt tgctcagacg cgtataggaa
     4681 aaaatggtcg tcagttcact ttttataagt ttcgctctat gtgtgtagat gccgaggcga
     4741 aaaaaagaga actcatggaa caaaatacca tgcagggtgg aatgtttaag gtggacgatg
     4801 atcctcgtat cacgaaaatt ggttgtttta tacggaagac tagcttggac gagctaccac
     4861 agttttataa tgttctaaag ggagatatga gtttggttgg cacacggcca ccaacagtgg
     4921 atgagtatga acactatacc ccagaacaaa aacgtcggct aagttttaaa cctggtataa
     4981 caggcttatg gcaggtcagt ggacgaagtg aaatcaaaaa tttcgatgaa gttgtcaaat
     5041 tagatgtggc ttatatagat gattggacaa tctggaaaga tattgaaatt ttattgaaga
     5101 cagttaaggt agtattgatg aaggatggag ccaaataggg ggatatgttc atgaaattgc
     5161 ttcattttag tgaagttggc ggtggagttg ataggtatat taagttatat ttaaaatatt
     5221 cagataaaga acattttaaa aatattgtgg taggatcaga tcagcttaat agacaaacat
     5281 atgaacaaga atataatata aagttttatc acattgatat ctatagaagc ttgtctccaa
     5341 taaagctttt acgcgcgatt aaacaattta gaaaaatatt gtatctagaa agacctgata
     5401 tagtatatct gcacagtact tttgcaggtg tagtaggcag gttagcttct atgggtttgt
     5461 cgtgtaaagt agtatacaat cctcacggat ggtcttttaa gatggatgtt tctaagatta
     5521 agcaattcgt ttataaaaat attgaaaagt ttttgtctta tcttacagat aagtatatat
     5581 taatctctaa atctgaatat gaagcggctc aatctttaaa aatacccctt aagaaattga
     5641 ctttagtgta taatggaata gagattgatg aagattttaa cgaaaat
//
LOCUS       DAVKZB010000103         5666 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKZB010000103 DAVKZB010000000
VERSION     DAVKZB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.103"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(72..624)
                     /locus_tag="VXA80_001685"
                     /pseudo
     CDS             complement(72..624)
                     /locus_tag="VXA80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(700..1476)
                     /locus_tag="VXA80_001686"
     CDS             complement(700..1476)
                     /locus_tag="VXA80_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354144.1"
                     /translation="MFALLDNSVFLKRNKIWKKTLTYPVEREEITYKRKKAKGKRQAI
                     LAQFDSEEVHHRLENCICPDCQGELKEIGASLQRQELVFILAQLKRVNHIQHAYKCQT
                     CSKNNPSDKIVKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPI
                     TRKEIANWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLS
                     GKSEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1400..1594)
                     /locus_tag="VXA80_001687"
     CDS             complement(1400..1594)
                     /locus_tag="VXA80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354145.1"
                     /translation="MKIIQQQSATIDSLTNELSLLREQVAYLTQKLSGKSSEKSVCPF
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1659..2009)
                     /gene="tnpB"
                     /locus_tag="VXA80_001688"
     CDS             complement(1659..2009)
                     /gene="tnpB"
                     /locus_tag="VXA80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0354146.1"
                     /translation="MTIHLSSLGQVYLVCGKTGMRQGIDSLAYLVKTHFELDSFSGQV
                     FLFCGGRKDHFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(1990..2175)
                     /locus_tag="VXA80_001689"
     CDS             complement(1990..2175)
                     /locus_tag="VXA80_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354147.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            complement(2584..3810)
                     /locus_tag="VXA80_001690"
     CDS             complement(2584..3810)
                     /locus_tag="VXA80_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0354148.1"
                     /translation="MAKTRYSGVFVDTKGKYFYETELGIDRISGKRIRKKSRKDSNGK
                     PFSTAREAYLELTRVKREYHKAHTYANYNMTVKQFILDCYIPYYKLYVEESTYAVRIK
                     NLEKIAERFYNKTLRGLTIEDIQNYRIWLLSSKDENGSGYSQAYASLIFGMFRKSLDY
                     AVEMGYLETNISKKAKAIPKGKAIVPYWTKVEFERVIAQICLDDFYEHLNFVMLWTFF
                     MTGIRVNEGMALWWNDIDFDKKRMRVHHMLILKSRKNWQRNAYTKTSDGKRIISLDDD
                     TISILKRWKDRQKEIGLGNETDFIFTYDSLPMIKSTLARIIQRYSELANVKRIQGKGL
                     RHSNASYLINEFNVSVLILSKRLGHSSPEITLKHYSHLWRGADESIAEIMSGNISIHT
                     APKTKIQFNGNQNLKR"
     gene            complement(3814..4008)
                     /locus_tag="VXA80_001691"
     CDS             complement(3814..4008)
                     /locus_tag="VXA80_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEV0354149.1"
                     /translation="MKPLISKDDLIKLGYKKHTAISIIQAVKQDLVYNKGIPFYNNRR
                     LGLVPRERVENLIGVKLDEE"
     gene            complement(4016..4828)
                     /locus_tag="VXA80_001692"
     CDS             complement(4016..4828)
                     /locus_tag="VXA80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354150.1"
                     /translation="MTRELYNTKIIQYNSNTFEVIYYRKAKFRRLGETDTTVSNTSKS
                     PSEKELYQQANRIKRRIRHYMLCNDFDLFWTLTFNDEKINSHDYLIVKKKIRTWLKAQ
                     REKYGKFHYIFIPELHKNGRLHFHGVTGGFSPKLTKARNTKTGRLINKNGKQVYNVDS
                     YQLGFSTVTKIDSSEKVANYITKYITKDLLAIPSGYKQPKYFSSRGLIKPIVSYENLE
                     RNYFEGLTPSFSVGHLDNLEYKEDIAIYNLSIDDNEEIIQNSTDTRIKIRKE"
     gene            complement(4932..>5666)
                     /locus_tag="VXA80_001693"
     CDS             complement(4932..>5666)
                     /locus_tag="VXA80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV0354151.1"
                     /translation="DIASEANQQIIIAPSGAGKSLYLATLAGLLIKQGHSISLIDAKQ
                     TSLGATFENLGIPVARNAEEIILLLEQMVFEMEDRYKRYFAFSSVDFGTTYKHFSLPA
                     HYLIFDEVLAALECGTSAQQKEMVRLLKILALKSRASGQGLLVLASQKLLASDLPRAV
                     TEQCQTRIILGNSSSISEETFYSVMGKEKIDLLAEYRGGVGKGYILTPKTNGIKYFET
                     PYFDLDSIDFKNKIRDFQTIPRKQIE"
BASE COUNT         1655 a         1109 c          941 g         1961 t
ORIGIN      
        1 ttaggaaagg aaaattgatt tatagaaata ttttagcagt cgatgcgtac tgttatagat
       61 tcaacgcact ataattgatg acgtttagct gtttccaaca aactcataat aatagctctt
      121 gctttagctc cttcaaaact ttgagaaaat aaccaccctc gcttttttaa actgagctca
      181 ggctaaaaga gtccactgga ctcttttact ccgtcccata accaatgatt taatggcgcg
      241 ttcagctaga ttattggaaa ggaccagatg tccgtctttt aaaatggtct taaaggtttc
      301 tttatacttg aggctgtatt caattgccct tcctagtttt gaacctgcta aaactgactg
      361 acggcggcac caagcaaaga agtcttccat taggggctgg agatgttctt gacgtttctg
      421 tagtcgttca tcagctggca aagcctccca gtctctttcc aaggaaaata actgatcaca
      481 ataagctaaa cctttagctc ctaaggatga tttatctgct tgcttggggg tcgcttcaaa
      541 aaatttcctt ctcacatgcg cccaacatcc aacaagtgca gcttcttcca gttgacgata
      601 agcttctagc ggttcgcagt ttaccaagct tagcgtcgcc ttgctcctaa accttggact
      661 gctatcgcat aggcagaact agaggactaa agtcctaagt tactgccgca acatatcaca
      721 atgaacatag ccagaataat ctcctaggaa ttcttgtact actgaaccac ttcgacactg
      781 atcatggtgg taaagcgtaa tcccttgttt ctctgattta cctgacaaaa aagtccaata
      841 gtaagtcagc tgactatcac tttctaagac ccgataagag gtttcatctg catgaagaag
      901 aggttgttct aacaactttt ctcgtaaaag gttataaagg gactctaaat agtattgact
      961 cgccttgata tgccaattag caatttcctt acgtgtgatt ggtaaaccca tcctagccca
     1021 atcttcttct tggcgataat taggtacctt cagattaaac ttctgatgga tggtgtgagc
     1081 gataatagaa gctgagccaa ggctatgcgc caaaggggct ttaggaatag gagctttcac
     1141 gattttatca cttggattat tcttactgca tgtttggcat ttgtaagcgt gttggatatg
     1201 attcactctt tttaattgcg caagaataaa gaccaattct tgtcgttgaa ggctcgctcc
     1261 aatctctttt agctctccct gacagtcagg gcaaatgcag ttctctaagc gatgatgaac
     1321 ttcttctgaa tcaaattggg caagaatagc ttgacgtttc cctttagctt tcttacgttt
     1381 ataggtgatt tcttctcttt caactgggta agtcagagtc ttcttccata ttttgttcct
     1441 cttcaaaaag actgagttgt ccaaaagggc aaacactttt ctcagaggat tttccagaga
     1501 gcttttgcgt tagataagcc acttgttcac gcagaaggga aagttcatta gtgagactat
     1561 caattgtagc actctgttgt tgaatgattt tcaatcttct tccatgagct tccctctctg
     1621 ttttctaact ttattatact aaaaagaaag tcctcatttc aatagaaatc acgactttct
     1681 gataaattta ttttgggagt gatagaaaag cccttcataa gccagtctac ttgttcaggt
     1741 gtgagagctt tgacatcctt ttctgtactg ggccaagtca gtctgccgtt ctcaaagcgt
     1801 ttatataata gccaaaatcc ttgaccatcc cagtaaaggg ctttaaagtg gtctttacgt
     1861 ccaccacaaa agagaaaaac ttgacctgag aaagaatcca attcaaagtg ggttttaacc
     1921 agataagcca gtgaatcgat tccttgtctc atacccgttt tcccacatac gagatagacc
     1981 tgccctaggc tagatagatg aattgtcata gagcaacacc ttatccaaaa gctgttctat
     2041 catttcgaga ttgagagatt gaaaaaaact tacttcaagc ttgccaatac gaattttaag
     2101 cagaatatca tttctctttt tagaatcaaa acgacgagat tgtggaatct ctacaggaac
     2161 aataggttgt ggcattaaaa tatcctccaa tagttttact ttactaatag tatacaggag
     2221 gagctccatg aatgatagat accttgttat gacgcgctta ccatattaga aagttggctc
     2281 tctaattaaa ttaattctca ttatatatat caaaagaccg cagcttgaaa actacggtct
     2341 aattttttgt ccaaatgtaa accacttgga actattattc aatttattct ttttaataaa
     2401 tgttcccgtt ttaatcccag ttacagtttt taatcctctg taaccgttga ttttacaaag
     2461 cttttaattt ttttgcgatt actcccactc aattaacaat ttcataggtt tagtgccaga
     2521 aaaccttgat ttattaaggt ttacaatgcc ttttaaatat atttggcggg atttctggcg
     2581 gggttatctt tttaggtttt gattcccgtt gaattgaatt tttgtttttg gagctgtatg
     2641 tatggatata ttcccactca ttatttcagc aatagattcg tcagctccgc gccaaaggtg
     2701 tgagtaatgt ttcaaagtaa tttctgggct agaatgacct aaccttttcg acaaaattaa
     2761 gacagataca ttaaactcgt ttatcaaata agaagcgtta ctatgacgaa gtcctttacc
     2821 ctgtattcgc tttacattag ctagttcaga atatcgttga ataattctag ctagtgtaga
     2881 ttttatcatt ggaaggctat catatgtaaa tataaaatca gtttcattgc ctaaaccaat
     2941 ttctttctgt ctgtctttcc aacgctttaa aatagaaatc gtatcatcat ctagggaaat
     3001 aatacgtttg ccatcacttg ttttggtata ggcatttctt tgccagtttt tccttgattt
     3061 tagaataagc atatgatgaa cacgcatacg ctttttatca aaatcaatat cattccacca
     3121 aagtgccatt ccttcattta ctcgaatacc tgtcatgaaa aaagtccaaa gcataacgaa
     3181 attaagatgt tcataaaaat catcaagaca gatttgagca ataacacgct caaattctac
     3241 tttagtccag tatggaacga tagcctttcc tttcggaata gctttggctt tcttagagat
     3301 attagtttcc aaataaccca tttctacagc ataatcaaga gactttctaa acataccaaa
     3361 gattagacta gcgtaggctt gagagtagcc agaaccgttt tcgtctttgc ttgacagtaa
     3421 ccatatccga tagttttgaa tatcttcaat agtcaaacca cgtagtgtct tgttataaaa
     3481 tcgttcagca atcttttcaa gatttttgat tctgacagca taagtacttt cttccacata
     3541 caatttataa tatggtatat aacaatctaa aataaactgc ttgacagtca tattgtaatt
     3601 agcgtaagta tgagctttgt gatactctcg ttttacccga gtcaattcta aatacgcctc
     3661 tctggcagta gaaaaaggct taccgttgct atcttttcta cttttctttc taatacgttt
     3721 accactgatt ctatcaatgc ctagctctgt ttcatagaaa tattttcctt tcgtatcaac
     3781 aaacacaccg ctataacgtg tttttgccat ttgttattcc tcatctagtt ttacaccgat
     3841 aaggttttct accctttccc gtgggactag tccaagtcta cggttattgt agaatggtat
     3901 acctttatta tacaccaaat cctgctttac cgcttgaata atactaattg cagtatgttt
     3961 cttatatcct agttttatca agtcgtcttt actaatcaat ggtttcatac gcttactatt
     4021 cctttctgat ttttattctt gtatcagttg aattttgaat aatctcctca ttgtcatcta
     4081 ttgatagatt gtaaatcgca atatcttctt tgtactcaag attatctaaa tgacctactg
     4141 aaaaactggg ggttaatccc tcaaaataat ttctttctaa attttcatag cttactatcg
     4201 gttttattaa accacgagat gaaaaatatt ttggctgttt atatccactt ggaatagcta
     4261 gtaaatcttt tgtgatatat tttgtaatgt agttagcaac tttttcagaa gaatcgattt
     4321 ttgtaacagt actaaatcct aattgatatg aatcaacatt atagacttgc ttgccattct
     4381 tatttatcaa tcgaccagtt ttagtatttc ttgcttttgt taattttgga ctaaaaccgc
     4441 ctgttactcc atgaaaatga agccgtccat ttttatgtaa ttctggtatg aaaatgtagt
     4501 gaaattttcc atatttttca cgctgagctt tgagccaagt gcgtattttc ttttttacga
     4561 ttaaataatc atgggaattg attttttcgt cgttaaaagt taatgtccaa aataaatcga
     4621 aatcattgca cagcatgtag tgacgaatac gcctttttat cctattggct tgctgataga
     4681 gttctttttc acttggactt ttagaagtat tagatactgt agtatcggtt tctcctagtc
     4741 ttctaaattt tgcttttcga tagtatatga cctcgaaagt atttgaatta tattgaatta
     4801 tttttgtgtt atataattct ctagtcatta gtcctccgct tatcatgcct gcatgtcaag
     4861 tagcaggcta atatacacat ctagcgagtc ctggcttgct tatgagcaag ccggctcact
     4921 agatagttag ttcattctat ctgctttctt ggaatagttt gaaagtccct tattttattt
     4981 ttgaaatcta ttgaatctaa atcaaaatac ggagtctcaa aatattttat tccgtttgtt
     5041 ttaggagtta aaatatatcc ttttccaact cctcctctgt actccgccaa aagatctatt
     5101 ttctctttac ccatgacact gtaaaacgtt tcttcagata tactggaact attccctaaa
     5161 ataattctag tttggcactg ctctgtgact gcacgcggta aatcagaagc aaggagcttt
     5221 tggcttgcaa gaacaagcaa tccctgtcca gaagcccgtg attttaaggc aagtattttt
     5281 agtagcctaa ccatttcttt ttgttgcgct gatgttccac attccaaagc tgccagcact
     5341 tcatcaaaaa ttaaataatg tgcaggtaaa gaaaaatgtt tgtacgtggt cccaaaatca
     5401 actgaagaaa atgcgaaata tcgcttatat ctatcttcca tttcaaatac catttgttct
     5461 agaagtagaa taatctcttc cgcattacgt gcgacaggaa tacctagatt ttcaaatgtc
     5521 gcacctaaag aggtttgttt agcgtcaatt aaacttatag aatgaccttg tttaattagt
     5581 aacccagcta gtgtggctag ataaagtgac ttcccagcac cactaggagc tattataatc
     5641 tgctgattag cctcactagc tatatc
//
LOCUS       DAVKZB010000104         5650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKZB010000104 DAVKZB010000000
VERSION     DAVKZB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.104"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(143..1813)
                     /locus_tag="VXA80_001694"
     CDS             complement(143..1813)
                     /locus_tag="VXA80_001694"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV0354152.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATFRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2043..2732
                     /gene="coaB"
                     /locus_tag="VXA80_001695"
     CDS             2043..2732
                     /gene="coaB"
                     /locus_tag="VXA80_001695"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV0354153.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     IDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2744..3295
                     /gene="coaC"
                     /locus_tag="VXA80_001696"
     CDS             2744..3295
                     /gene="coaC"
                     /locus_tag="VXA80_001696"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV0354154.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3279..3842
                     /locus_tag="VXA80_001697"
     CDS             3279..3842
                     /locus_tag="VXA80_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0354155.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4106..4633
                     /locus_tag="VXA80_001698"
     CDS             4106..4633
                     /locus_tag="VXA80_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0354156.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4645..5160
                     /locus_tag="VXA80_001699"
     CDS             4645..5160
                     /locus_tag="VXA80_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV0354157.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5157..5624)
                     /locus_tag="VXA80_001700"
     CDS             complement(5157..5624)
                     /locus_tag="VXA80_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0354158.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT         1670 a         1243 c         1030 g         1707 t
ORIGIN      
        1 catataccta cggcaaggtg aagctgacgt agtttgaaga gattttcgaa gagtataaat
       61 cttattgatg aactgcttgc agtctgagaa aaaatgagct tggatattat ttccaaactc
      121 acttaaagtc aatttcaatc cactagaaca agcctagtac agttccatcg ctttcaacat
      181 ccatgttgag agctgctgga cgttttggaa gacctggcat ggtcataaca tcaccagtta
      241 aggcaacgat gaagcctgca cctaattttg gtaccaattc acgaatggta atttcaaagt
      301 tttctggtgc tccaagcgca tttggattgt ctgagaaact gtattgagtt ttagccatac
      361 agattggcaa tttgtcccaa ccgttttgaa cgatttgagc aatttgtgtt tgagatttct
      421 tctcaaagtt cactttgcta ccacgataga tttcagtgac aattttttca atcttttctt
      481 ggacagaaag gtcattatca tacaaacgtt tatagttagc tggattttca gcaattgtct
      541 taacaactgt ttcggcaagt gctactccac cttctgctcc atcagcccag acactagcca
      601 attcaactgg tacatcgatt gaggcacaga gttcttttaa gactgcaatt tcagcttctg
      661 tatcagatac aaattcgtta atagctacaa ctgctggaat accgaactta cggatatttt
      721 caacgtggcg tttcaagtta gcaaaacctg cacgaactgc ctctacattt tcttcagtca
      781 gagcgtcttt agccacacca ccattcatct taagggcacg aaaggttgcg acaataacaa
      841 ctgcatctgg agatgttggc aagtttggtg tcttgatatc aaggaatttc tcagcaccaa
      901 ggtccgcacc aaaaccagct tcagtaacag tgtaatcagc caagtgaagg gctgttgtcg
      961 tcgccaaaac agagttacag ccatgagcga tattggcaaa tggaccaccg tgtacaaagg
     1021 caggtgtacc gtaaattgtc tgaaccaagt ttggcttaat agcatccttc aaaatcaaag
     1081 ccaaggcacc ctcaacctgc aaatcaccta cagaaacagg cgtacggtca tagcgataac
     1141 caataacgat attcgccaaa cgacgtttca agtcctcgat gtccgttgcc aagcaaagaa
     1201 ttgccatgat ttctgaagca actgtaatat caaaaccgtc ctcacgtgga ataccgttta
     1261 gaggaccacc aagaccaaca gtcacatggc ggagcgcacg gtcgttcaag tccacaacgc
     1321 gtttccagag gatacgacgt tgatcaattc ccagctcatt cccttggtgc aagtggttgt
     1381 caatcaaggc agaaagggca ttgttggcag ttgtaatagc atgcatatct ccagtaaagt
     1441 ggaggttgat gtcttccatt ggcagaactt gtgcataccc accaccagca gcaccaccct
     1501 tgatccccat gactggacca agagacggtt cgcggatagc aatcatggtt ttcttgccaa
     1561 tcttgttcaa ggcatccgca agaccaatgg taagcgtcga ctttccttca cctgcaggtg
     1621 ttgggttgat ggcagtaacc aagatcaatt taccgactgg attgctctca actgcacgaa
     1681 ttttatcaaa gctgagttta gccttgtact ttccgtacaa ctccaaatcg tcgtaagaaa
     1741 taccaagttt ctctacaaca tcaacaattg gcttcaactc aatactctgt gcgatttcaa
     1801 tatctgtttt cattcaaaat tcctctaacc tcttatatga taattcatta tatcacaaaa
     1861 caagattttt aacatcctaa aactctctaa acgttcgtaa atatctctgt ttttaaggct
     1921 tttagaatcc tttcttaaat tttatatggc tttatagttt gaaactatag taaatcttcg
     1981 tttttaccaa aaatttatca ctttcatttt acttaccgct tatttttgtg tacaatagtg
     2041 ctatgaaaat tttagttaca tcgggcggta ccagtgaagc tatcgatagc gtccgctcta
     2101 tcactaacca ttctacaggt cacttgggga aaattatcac agagactttg ctttctgcag
     2161 ggcatgaagt ttgtttaatt acgacaaaac gagctctgaa gccagagcct catcctaacc
     2221 taagtattcg agaaattacc aataccaagg accttctaat agaaatgcaa gaacgtgttc
     2281 aggattatca ggtcttgatc cactcaatgg ctgtttctga ctacactcct gtttatatga
     2341 cagggcttga ggaagttcag gctagctcca atctaaaaga atttttaagc aagcagaatc
     2401 atcaggccaa gatttcttca actgatgagg ttcaggtttt gttccttaaa aagacaccca
     2461 aaatcatatc cctagtcaag gaatggaatc ctactattca tctgattggt ttcaaactgc
     2521 tgattgatgt taccgaagat catctggttg acattgcacg aaaaagtctt atcaagaatc
     2581 aagcagattt aatcatcgcg aatgacctga ctcaaatttc agcagatcag caccgagcta
     2641 tatttgttga gaaaaatcag cttcaaacag tccagactaa agaagaaatt gcagaactcc
     2701 tccttgaaaa aattcaagcc tatcattctt agaaaggaaa actatggcaa acattctctt
     2761 ggctgtaacg ggttcaatcg cctcttataa gtcggcagat ttagtcagtt ctctaaaaaa
     2821 acaaggccat caagtcactg tcttaatgac tcaggctgct acagagttta tccaaccttt
     2881 gacactacag gtactctcac agaatcctgt ccacttggat gtcatgaagg aaccctatcc
     2941 tgatcaggtc aatcatatcg aacttggaaa aaaagcagat ttatttatcg tggtacctgc
     3001 aactgctaac actattgcaa aactagctca cggatttgcg gacaacatgg taaccagtac
     3061 agctctagcc ctaccaagtc atattcccaa actaatagct cctgctatga atacaaaaat
     3121 gtatgaccat ccagtaactc agaataatct gaaaacatta gaaacctacg gctatcagct
     3181 gattgctcct aaggaatccc tactagcttg tggagaccac ggacgaggag ctttagctga
     3241 cctcacaatt attttagaaa gaataaagga aactatcgat gaaaaaacgc tctaatattg
     3301 cacccattgc tatctttttt gctaccatgc tcgtgataca ctttctgagc tcacttatct
     3361 ttaacctttt tccatttcca atcaaaccga ccattgttca tattcctgtc attattgcca
     3421 gcattattta tggtccacga gttggggtta cacttggatt tttgatggga ttacttagct
     3481 tgacggttaa cacgattacg attctaccga caagctacct cttctctccc ttcgtaccaa
     3541 acggaaacat ctactcagct atcattgcca tcgtcccacg tattttgatt ggtttaactc
     3601 cttacttagt ctataaactg atgaaaaaca agactggtct gattttagct ggagcccttg
     3661 gttccttgac aaatactatc tttgtccttg gaggaatctt cttcctattt ggaaatgttt
     3721 ataatggaaa tatccaactt cttctggcaa ccgttatctc aacaaattca attgctgaat
     3781 tggtcatttc tgcaattcta accctagcca ttgttccacg actacaaacc ttgaaaaaat
     3841 aaaaacaatc tccgctttca aacctgaagg tggagatttt gctctataca gtttcttttt
     3901 aatgaaatct ttactatagt aaaacgaaat aagaacagga caaatcgatt tctaacaatg
     3961 ttttagaagt agaggtggac tattctagtt tcaatctact ataactttta actaaaaaat
     4021 gatctataaa cccaagtaaa ttcttgcttt attgtctggt ttattgtacc atactagtgt
     4081 atatgcagtt aaaaaggaga ttcttgtgaa tacacggaaa aagacacaat ttatgacaat
     4141 gacagccctt ttaacggcta ttgcgatttt gattccaatt gttatgcctt tcaagattgt
     4201 cattccacct gcttcctata ctttggggag ccacatcgct atttttatag ccatgttctt
     4261 gtcgcccttg atggcagttt ttgtcatcct agcctctagt tttggatttt tgatggctgg
     4321 ctatcccatg gttatcgttt ttcgggcttt ttcccatata tcttttggtg ctttaggagc
     4381 tctttaccta caaaaattcc ccgataccct agataaacca aaatcttcct gggtttttaa
     4441 ttttgttttg gctgttgttc atgcccttgc tgaagtatta gcctgtgtcc ttttttacgc
     4501 aacttcgggt accaatgtag aaaacatgtt ctatgttcta tttgtactag ttggatttgg
     4561 cacaattatc catagtatgg tagactatac attagcacta gctgtctata aagtgcttcg
     4621 aaaacgccgt taaaaggaaa agctatgaca aaagatcgca aacaagccct gctccaactc
     4681 ttgaaagaag ctcctaaagc ccttaatggc caaagtttgg cggaacattt tcatgtcaca
     4741 cgtcaggtca ttgtgcagga cattgcaatt ttgagagccg atggcgctcc tatcctatcc
     4801 accaatcgtg gctacatcta taagcaaatt gataccaatc cttatgttca caaacttttc
     4861 aaagtgaaac atgaagttga agaaatcggt caggaactcc ttgctattgt cgataatggt
     4921 gggcatgtcc agaatacctt gattgaccat cccgtttatg gagaaattga aaccttgctt
     4981 aaactgtctt gtcgcagaga tgtgcaacat tttctagaac aagtcgagca ttctgacttt
     5041 agacctctat ctgaattgac agatggcatc cattaccacc tagtcgaagc cgaaacacaa
     5101 caagacctcc actatatcga ggaggccttg gatcagctag gttatttagt aaaagactag
     5161 aagattttct tttcccaatc gtcttctgta cggcgagggt agaaaaactc tgacttgtcg
     5221 ggagcttcca tcatttccat caatggtagc atatcatagg ccagatttaa gtttggaatc
     5281 tggtcttttt gcacccaaga aacttctccc tcttctgaag attgaagggt accagagaac
     5341 tcagtcgcct tataacaaat gacaatatag cgcccacctg tatctagtgg ccaattttta
     5401 atgccgacaa gttgaggatt ttggatagtc aaccctgttt cttcgtagat ttcacgaatg
     5461 acagactccg caaaagcctc atcattttct acatgacctc caggaaaggc ataaccagac
     5521 cagcgattgt tttcaggggc gcgatactgc atcaccacgc gctgagtttc gaggtcttca
     5581 atcagacaga tatttgttaa aattgttaat tgggaacggg acataaattt actcgctttc
     5641 taatttatta
//
LOCUS       DAVKZB010000105         5535 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKZB010000105 DAVKZB010000000
VERSION     DAVKZB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.105"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            293..1243
                     /locus_tag="VXA80_001701"
     CDS             293..1243
                     /locus_tag="VXA80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV0354159.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQDRLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLDAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            1245..1988
                     /locus_tag="VXA80_001702"
     CDS             1245..1988
                     /locus_tag="VXA80_001702"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV0354160.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            1999..3795
                     /gene="pepF"
                     /locus_tag="VXA80_001703"
     CDS             1999..3795
                     /gene="pepF"
                     /locus_tag="VXA80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0354161.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            4042..4596
                     /locus_tag="VXA80_001704"
     CDS             4042..4596
                     /locus_tag="VXA80_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV0354162.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPDFPINRIHEVFDILGQLFKRMS
                     ILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            4707..5375
                     /locus_tag="VXA80_001705"
     CDS             4707..5375
                     /locus_tag="VXA80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV0354163.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLTIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         1560 a         1082 c         1324 g         1569 t
ORIGIN      
        1 tgacatcatt attctctatg gggtttcttt gggagaaact gataggtata tctggagtca
       61 gattgctgaa cgatcaatca gcggttctgt tcctgtcata atttaccact atgtacctca
      121 ttttgatccg gggaatccca ttagagcaaa acgcctatat agaaatgtag aagataaatt
      181 catacagaat agtggtatag acttagagtt agagaaaaaa ttaagagaca accttattgt
      241 cgttattgga aagactattt ttaatctgat ggaaaggtaa gcaaaataca taatggaaac
      301 atggcaagag ttaaaagtta cagtgaagcg tgagggagag gagttggttt ccaatctctt
      361 gattgagctg ggagcgcaag gtgttgcgat tgaagacagt atggactatg tgggaaatgt
      421 tgaccgcttt ggcgagattt tcccagaggt ggagcaacag gaagaaatcg tagtgaccgc
      481 ctactaccct gaaacggttg atgtagcagc agttgaagcg gatttgcagg atcgtctggc
      541 agaattgacc gactttatgg acctaggaga agtcaagatg ggcacgactg ccttggctga
      601 ggaagactgg gctgacaact ggaagaagta ttatgaaccg gcacgcatca cccatgacct
      661 gaccatcgta ccgtcatgga cggactatga ggcaacagca ggagaaaaga ttatcaagct
      721 ggatcctggt atggcctttg ggacagggac ccacccaaca accaagatga gcctctttgc
      781 cttggaacag gttcttcgtg gtggcgaaac agtgctagat gtagggactg gttcaggggt
      841 tctttctatt gcaagctcac tcctagatgc caaggagatt ttcgcctatg acttggatga
      901 tgtggcggtt cgtgtcgctc aggaaaatat tgagctcaac actggtatgg aaaatatcca
      961 tgtagcagca ggagacttgc ttaagggagt tgagattgaa gcagatgtca tcgtggctaa
     1021 tatcttggcg gatattctca ttcatctgac ggaggatgct tatcgcttgg taaaggatga
     1081 aggctacctc atcatgagtg gcattatcaa ggacaagtgg gacatggtgc gccagtcggc
     1141 tgagtcagct ggatttttcc tcgaaactca catggttcaa ggggaatgga atacctgtgt
     1201 ctttaagaaa accaaggata tctctggtgt gattggaggc tagcatgcag cagtattttg
     1261 taaaaggcag tgctatctct cctgtcacta tcgaggacaa ggaaaccagt aagcatatgt
     1321 ttcaggttat gcgcttgaaa gaagaggatg aggttacctt agtctttgat gatggcatca
     1381 agcgcttggc gcgcgtgctg gatatggaaa atcgtcagtt tgagttggtc caagaattaa
     1441 atgataatgt ggaactacca gtccaagtga ccatcgcatc tggatttccc aagggagaca
     1501 agctggagtt catcgctcaa aaagtaaccg aactgggtgc tagccaaatc tgggcctttc
     1561 ctgcagactg gtcagttgcc aagtgggatg gcaagaaatt gggtaaaaaa gttgaaaaac
     1621 tagaaaaaat tgcccttgga gcagccgagc aaagcaagcg taatattgta ccaagtatta
     1681 aacttttcga gaaaaaagca gattttctag ctcagctgga ccagtttgac tttatcatag
     1741 tagcctatga agaatcagct aaagaaggag aagccgctgc gcttctgcaa gcagtcactg
     1801 gtcttgaaac aggagccaaa ctgctcttta tctttggtcc agaaggcggt ctctcacctg
     1861 cagaaatcga aagttttgaa gctaagggag ccgttttggt aggcctaggt cctcgtattt
     1921 tgcgagcaga aacagcaccg ctttacgcct tatcagccct tagtgtttta gtagaattag
     1981 agaaataaga ggaagaaaat ggaacaaaaa caccgttcag aatttccaga gaaggaactc
     2041 tgggacttaa cagccctata ccaagaccgt gaggatttct tgcgtgcaat cgagaaagct
     2101 cgtgaagaca tcaaccagtt tagccgtgat tacaagggca atcttcacac ttttgaggat
     2161 ttcgagaagg cctttgcgga attagaacag atctacattc agatgagcca tattggcaac
     2221 tatgctttta tgcctcagac gacggactat agcaatgacg aatttgccaa tattgcccaa
     2281 gctgggatgg aatttgaaac agatgccagc gtagccttga ccttctttga cgatgccttg
     2341 gtggtagcag acgaggaagt cttggaccgt ttgggtgaat tgcctcactt gacggcagct
     2401 attcgtcagg ccaaaatcaa aaaagcccac tatctagggg ctgatgtgga gaaggccttg
     2461 accaatctcg gtgaagtttt ctatagtccg caggacattt atactaagat gcgagctggg
     2521 gattttgaaa tggctgactt tgaagcccat ggcaagacct ataaaaacag ctttgtcacc
     2581 tatgagaatt tctaccaaaa tcacgaggat gctgaggttc gtgagaaatc cttccgttcc
     2641 ttctcagagg gacttcgtaa gcaccaaaat acggctgcag cagcctatct ggctcaggtc
     2701 aagtctgaaa aactcttggc tgatatgaag ggatacgact ctgtatttga ttatcttcta
     2761 gctgaacaag aagtggaccg tgtcatgttt gaccgccaga ttgacctcat catgaaggac
     2821 tttgcaccag tcgctcagag atacctcaag catgttgcca aggtaaatgg tcttgaaaag
     2881 atgacctttg cagactggaa attggacttg gacagcgccc tgaatcctga agtgactatt
     2941 gacgatgcct atgatttggt catgaagtcg gtagaacctt tggggcaaga atattgtcag
     3001 gaagttgctc gttaccaaga agagcgctgg gtggactttg ctgctaacag tggcaaggat
     3061 tccggtggtt atgcggcgga cccatatcgc gtacaccctt atgtactcat gagctggaca
     3121 ggccgtttga gcgatgtcta taccttgatt catgaaatcg ggcattctgg tcaattcatc
     3181 ttttcagaca atcaccaaag ctatttcaac gcccacatgt cgacctatta tgttgaagca
     3241 ccgtcaacct tcaatgaatt gctactcagt gattacttag agaaccagtc taatgaccca
     3301 cgtcaaaaac gcttcgctct ggctcatcgc ttgacagaca cctacttcca taatttcatc
     3361 acccacctct tggaagccgc cttccagcgt aaggtgtata cattgattga agaaggggag
     3421 acctttggag caagcaagct caacagcatt atgaaggaag ttttgacaga tttttggggc
     3481 gatgccattg aaatcgacga cgatgcagct ttgacttgga tgcgccaagc tcactactat
     3541 atgggcttgt atagttacac ttactctgct ggtcttgtca tctcgacagc tggttacctt
     3601 catcttaaaa actcagaaac tggagctgaa gactggctca atctcctcaa atcaggtggt
     3661 agcaagacac cacttgagtc agccatgatt atcggtgctg atatttcaac agacaaacca
     3721 ctccgtgata ccatccaatt cttgtctgac acagttgacc agattatcgc ctacagtgct
     3781 gagttggggg agtaagggga atagaaaaat tttctagaat gatgtttatg ggcttgttaa
     3841 gcgtcattct atttgtctta gttgtagcta ttttcaaaaa tgtcgttgaa ggtcgtgatg
     3901 ttaaatttga acttactgta gaactggtat tgatagttat ttacgtcatt ttatatcaga
     3961 ttgtatttaa ttgagaattc aatattgagt catttaagac tgcctttttg aacagttgct
     4021 agaaatgtag aggtattgac catgtatcaa gatttactaa gaaaaatagc agaagaaaaa
     4081 ccaaattata accaggaaga aatccagtgg ttgtttgatc atttgggaaa tccttctcca
     4141 gaaattcgtg atgacctttc aaatcaaggc ttgcactatc tttcaaaaga aaaggataca
     4201 acaggtttct caagtcagta tggttgggtt cattcttttg cacatggagc cgatttactg
     4261 acagaggtgg tctgtcatcc agactttcct ataaacagaa ttcatgaagt atttgatata
     4321 cttggccaac tatttaaaag aatgtcgatt ctctttacag atgatgagga ttggcgttta
     4381 gcaagagtga tctatgaacc tattttacaa gggaagttgg agcaagaaca agtagcttct
     4441 tggataaaaa ctgttgattt tccgatagaa gaaagggagg atttttacaa attttccaac
     4501 tttagatcct gtctggtgga agtctatgtc caacttgacc agagaaatag tttacaagat
     4561 gacctgaaag aagctatcca gtcttttcaa tactagggat tagctaatag ttctatgatt
     4621 ttcaaaaaat ttccaatcaa ttttcttgaa accctttcct acttttgata gactagtatc
     4681 tagtttttga aaaaaggaga aagaaaatga aaaaatttgt tgctgagtta atcggtacgt
     4741 tcatgcttgt gttcgttggg acaggagctg ttgtttttgg aaatggtctt gatggccttg
     4801 gtcaccttgg aatcgccttt gcctttggtt tggcaatcgt ggtggcagcc tactcaatcg
     4861 gaactgtttc aggtgctcac ttgaacccgg ctgtttcgat tgctatgttt gcaaacaaac
     4921 gtttgtcatc ttcagaactt gtaaactaca tccttggtca ggttgttgga gctttcatcg
     4981 cttctggcgc agtcttcttc ctcttgggta attcaggtat gtcaactgct agtcttggtg
     5041 aaaatgcctt ggcaaacggt gtcactgtct ttggtggttt cttgtttgaa gtcatcgcaa
     5101 ctttcttgtt tgtcttgact atcatgactg tgacttcaga aagtaagggc aatggcgcga
     5161 ttgctggttt ggtaatcggt ttgtcattga tggcgatgat tcttgtcgga ctgaagatta
     5221 ctggactttc agtaaaccca gctcgtagct tggcaccagc tgtcttggta ggcggcgcag
     5281 cccttcaaca agtttggatt ttcatccttg caccaatcgc tggtggagtt cttgcagccc
     5341 ttgttgcaaa aaatttcctt ggaacagaag aataattgaa actcaaaaag ccttgctcct
     5401 catcttgagg aacagggctt tttcgtatga tactcttcga aaatctcttc aaaccacgtc
     5461 agcttcacct tgccgtaggt atggttactg acttcgtcag tttcatctac aacctcaaaa
     5521 cagtgtttta agctg
//
LOCUS       DAVKZB010000106         5405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKZB010000106 DAVKZB010000000
VERSION     DAVKZB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.106"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..233)
                     /locus_tag="VXA80_001706"
     CDS             complement(<1..233)
                     /locus_tag="VXA80_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354164.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFK"
     gene            complement(508..2247)
                     /locus_tag="VXA80_001707"
                     /pseudo
     CDS             complement(508..2247)
                     /locus_tag="VXA80_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(2410..3294)
                     /locus_tag="VXA80_001708"
     CDS             complement(2410..3294)
                     /locus_tag="VXA80_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV0354165.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVDTENGNYVGEASHVLVLLTNYFADKKIFEENKDDYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(3406..4260)
                     /locus_tag="VXA80_001709"
     CDS             complement(3406..4260)
                     /locus_tag="VXA80_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV0354166.1"
                     /translation="MLGVIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     TWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(4318..5076)
                     /locus_tag="VXA80_001710"
     CDS             complement(4318..5076)
                     /locus_tag="VXA80_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0354167.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(5138..5326)
                     /locus_tag="VXA80_001711"
     CDS             complement(5138..5326)
                     /locus_tag="VXA80_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV0354168.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
BASE COUNT         1647 a         1147 c          814 g         1797 t
ORIGIN      
        1 tctttaaaat taaataaagg tgtatccggc ttagcattcc ataaaataat caaccacatt
       61 ataacattat aaagctgctt tgcagtttca ttccactttt caggttgtaa atttccactc
      121 cctgtattgt catattcttg tctattattt acatgagaat cacctacaag cggattaaaa
      181 agaggtagcc ctttgtgatt aattagttct aaaaaatccc ttgccgcacc catgcaacac
      241 tgaataactt ccttcttgcc atcaatttta ctctcagcga taaatacata atattcatct
      301 tcaatagttg ctcctgcatc actatgtttt tgttgatttg ctaacagctt aatatgtgct
      361 ttaggctaaa cttcaaaatc tctaacaatt ctttgtcttg tttcatgtcc tctacaattc
      421 atttaagttc tcctttacaa gtttagtaac tagccttatt atactatata agatatgaaa
      481 tgtaccataa tcttcatcaa ctagttttta gaaaatcaca aatccatctg gagagatgac
      541 aagctgattc tccaattctt ggcaattgct tgctagtgct actgcttctt tctctaaaag
      601 ataatcttct gttgattggt tgaatattgc tttgaggatc cgtccttcgt atttccattc
      661 cagagctact agatcagatt tgatttcttg aaggctatac ttgccatgcg aaatgattac
      721 tgacgcgtat ttccgaatct taatcagcct cttcataaag ttcagcatat cattgtcact
      781 tgatacacgt tcccaaggca tacaacgacg acaatctgga tctggtcctc cagtcaaggc
      841 tagctcggtt ccgtaataaa tgcacggtgt tcctttttgt aaaaagagaa aggctaaggc
      901 tgatttaacc agttgaacat cttcattggc cgtccacagg attcgctctg tatcatgtga
      961 atccaagaga ttaaacatga cctctgaaat ctgctgcttg taatacatag actctccatt
     1021 gatttcatcg atgaactggt ctgtcttctt aattcctcgt aagaaatagt ccttgatact
     1081 atcagataaa ggataattca tgacggcatg gaactcatct ccatttagcc aaggctgaga
     1141 tgtatgccag acttctccta ggatataaag atcaggattt ttagctaaaa ctgccttacg
     1201 aaaatccttc cagaactgat ggtcaatctc attagccaca tccaaacgcc aagcatcgat
     1261 attaaactct tcaatccaat aagtcgcaac ctttaaaaga taattcttga cctctggatt
     1321 ggctgtattt agcttaggca tatagtcctc gaaaccaaaa acatgatagg gtaagtctct
     1381 cttattaact agtttttcag ttgtcactgg gaattgttga atatggaacc aatccttata
     1441 agcagactgt tcaccatttt tgacgacatt ttttcattga agagattgcg aaccaatatg
     1501 attaaatacc gcatccagca tgactttcat gccacgatga tgcgcttgat ccaccagttc
     1561 ccgaaaggtc tccttgtctc caaaatgacg gtcaatttca aagtaatctg tcgtattgta
     1621 cttgtgattg cttgtagatt caaagatggg acaaagatat agtccagtaa tacccaagtc
     1681 ttgcaagtaa tccatatgat caataatccc ctgtaaatca ccaccaaaga aatcatcgct
     1741 cttaggtgtg acagatgaat cccagtctaa agtcccttct gggtttaata gagcattgcc
     1801 attggcaaat ctttcaggaa atatctgata ccataccgta tttgaaaccc cgtcaggaac
     1861 cttgcaggca tcaatctcat gaagataagg caacttaaat ccattcccaa ttgcatgaag
     1921 attttctaga gaattttcca cacacccttt atcgccatac aaaatatttt gaccttctgt
     1981 atctctgagc tcaaagagat actggatacg tgcaaagtca actgacactt caacctgcca
     2041 atagtcaaat aaggtaccag aagttatctt gaccatttct tttgtatcct gataaaactc
     2101 ctccataaag ataaaagggt ccccatagtg caagttgatg ctttcaatgt cccctttctt
     2161 agttcgaatt cgaatatgga gtttcttatc cttataaaga taggcatact ccgactcagg
     2221 cctatggtaa atagcactca attccatcaa tttgtctcct aaagtattca ttcaaatttt
     2281 acgcaaacgt tttcaaactc aataaatcca gtatactatt tttgtaaact atttgcaagg
     2341 aatgtccgtt gttaaaggaa ctatctttta aagtatacaa aaagccttaa aaaaggcttt
     2401 ctctatatac taatcctctt tcggttttga aaatacttct actcgctgag ctaggacttt
     2461 gatttctaca ggtaggttat ctgatttatc tccgtcaaca tctgactcca attcactatc
     2521 tgaagagatt ttaatattac gcgctttgat atactcgata ttatcatttg cgacaacatc
     2581 cccttttaat aaatcaggaa tgacggataa tttggagaat atagaggcat ctttcagaat
     2641 caaaatgttg gcatagtcgt ccttgttttc ttcaaagatt ttcttatcag cgaagtaatt
     2701 tgtcaaaagg accaaaacat ggctagcttc accaacataa tttccatttt ctgtatcaac
     2761 cttaatgtta aagacctgat ctgtcatgac agacttcatg gtatttacag cataggttaa
     2821 aataccgaat tttgttttgt cctctatttc aacattgtga atcgcctcag gcagagaacc
     2881 gatactaaag atataaccaa aatagttgtc atttgcttta ccgatatcaa tcttattggt
     2941 taaatcaaaa tccagttcgt caattgcgcc atcgatgtct tgattgattt ccaaaagttt
     3001 tgtaatgagg ttacccgtac cgcctgggat aatccctaac ttaggaatgt agtctctctc
     3061 atcaatacct gaaatgactt cattgacagt tccatctcca ccaaacacaa ccactgcatc
     3121 atactgctca cgagaagctt cttcagcaaa atgtgttgca tccagcgctt tttcggtaat
     3181 tttggtttca acatattcaa agtattcttt tgctttattc tccagctttt ctttgtaatc
     3241 caaagccttc tcgccaccag aagtagggtt gataattacc attgcttttt tcattgattt
     3301 tatccttaat tttaaacaga aatgtttaca tttcgtcgta tgcaagtaaa tgtaatccta
     3361 ttatacaatg aaaatacaga aaagagaaat ctgacgtact ggagattaat acgcttttat
     3421 tctattttcc catcgcctaa ctacatcctt taagggttca tccaagtaag aataggcctt
     3481 atccttgatc caatcaggaa taccgtaagc tgcctctgct atgctacaag tgattgctgc
     3541 gagagtatca ctgtcgccac caagtgagat ggcatttctt atcgcatctt cgaagtctct
     3601 actttcaaga aaggcgataa tggcttgagg gacagtttcc tgacatgttt cgttaaaacg
     3661 atagttagga cggatttcat ctaaagtttg agatagattg taatcgtatt ctttttctat
     3721 ataatcctta attcgtcttt tacactctgt aggattgtcg ttaattgatt gctcgtactc
     3781 tctacaataa ccaccaaagt aaaaacgaca tagaaagata gcatcagctg tggccatagc
     3841 gcctttgaca ccttctggat gattatgagt tacctctgca gaaagactcg taagtcctct
     3901 agatgatggc catataccag ttttcgcata aaaaccacag tccatgatcc aagtacatgg
     3961 agaaatacgc atagctgatc cattcccaaa gctattataa ggctcacggt tatcgctgtt
     4021 tagccatgca ttaaaccgag caccgtaatc agcattcgga tacattctgc catatttctt
     4081 catcgcgtca atgaagtcgt ctttttgtcc accattcata attgcttctg caacagcaca
     4141 ggtcataacc gtgtcatctg taaaaaaaca gtccttccga aataaaggaa agtcctttgt
     4201 tttgatattg ttccattcgt aaacagaacc gacaatatct ccaataatta ctccaagcat
     4261 cagattcctc cttgttcatt ttgatgcttt ttatattggt tatctaccat atttatttta
     4321 gaaaataaca tcctgttgga ttttaaaaat ttcatttttt tcaaaatagg gttttaccat
     4381 ttctttccac ctagctctat gaaaattgat tgattttaaa ggagataggc cataatttcc
     4441 caatgcataa ccatcattta cttcaacaac aagtgttctg ccatcgcgag taacaccgat
     4501 atctagtcca taagctattg gcgcatcttt ccaacatgat atcgcttcat caattacact
     4561 tgcatcaaat tgtgcatgat aatcacctgt atagggtcga acatctaata cgcgaccatc
     4621 taacacaaaa caacgccatt cagctatgaa ttctacaacc tcactaatcc atataggata
     4681 gtcgaaaggt agaccaatac ctattaaatc atgggttcca ttaacaactc ttccagtaaa
     4741 gacttttgaa ccagctttag gcttaataaa ttttccccaa ttatcaggta tattcacaat
     4801 ctctcctaaa ataccagcat aaatctttcg accataaaac tctttaagct caataggata
     4861 gtcatgaacc ggaacgttta agcccatcat ttttagtaat gctctagtct ccattatata
     4921 atctacaact atatcttcac ttgttaactc ttttatttca gaaaaagatt gatataaaat
     4981 aacttcttct ccttgtaagt aggcacctac ttgagcattg tatttattaa ttgaaacctc
     5041 acttggtaat ttactttgtc taatataaac aaccatttca tcactcctat atcactagtg
     5101 ttacaccaat ttgtaaaaaa taatagcaat tttgctctta tttttttgag taaatagccc
     5161 ccataatatc atcgaaataa tcaacggtat ttaggagtaa ttcaataacc tgggactttg
     5221 ttagtcgcat tccccttcta tctctagcat cttctactaa attttcaagt ttctctagat
     5281 ttttatcatc caagctaatc attattctat ttttatcggt tgccattttc atcacctcaa
     5341 gttaattcta tcacaggtgt aacacttgtg tcaactggct tttataatac attagtttaa
     5401 aagtg
//
LOCUS       DAVKZB010000107         5390 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKZB010000107 DAVKZB010000000
VERSION     DAVKZB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.107"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            5..664
                     /locus_tag="VXA80_001712"
     CDS             5..664
                     /locus_tag="VXA80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV0354169.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            689..1756
                     /locus_tag="VXA80_001713"
     CDS             689..1756
                     /locus_tag="VXA80_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0354170.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1860..2870
                     /locus_tag="VXA80_001714"
     CDS             1860..2870
                     /locus_tag="VXA80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354171.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETKQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2882..4573
                     /locus_tag="VXA80_001715"
     CDS             2882..4573
                     /locus_tag="VXA80_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0354172.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4576..5286
                     /locus_tag="VXA80_001716"
     CDS             4576..5286
                     /locus_tag="VXA80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV0354173.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1761 a          757 c         1035 g         1837 t
ORIGIN      
        1 ttatatgaaa aacttaacaa aaattaagtt caaagagaat ggggaattta atcatttccc
       61 tgggaataca gttgtagcaa atctttatac taaacaagat ttgatggaag ttgttgatat
      121 tattcaatca cgttatagag aattaccatt tatcgataag tttactttaa ctccaaggaa
      181 ttctattcac atgactgtaa ttgaattgtt gtgccatgaa aatcgtgaaa cggagttttg
      241 gagcagtaat cttcctctag atacaccttt acaggaaata catgattact ttgctaaaca
      301 acttgaaatt tttccattgt tggatgaaga aattcatatg cgtataactg aaatgggaaa
      361 acaaaacata ctagttgaac ctgcagatga agcttctgca aagagattag aagaaattcg
      421 tacttatgtc tcagaaaaag caggtgtttg tttccctaat catgatagat atcaattcca
      481 tatttcaatt gggtatcttc ggattcctct aaccgaagag gaggaagaag agtttactaa
      541 agtcagagca gaattaactg aaattttatt agagaagatt ccaactatta ctgtaaaccg
      601 tattgactat actgtatttg aagatatgag acaatttgtt ccatatcacg aaaaatttaa
      661 ataaaataat aaaaaaggag aacttatcat gaaaaaaatg aaagtttggt ctactgtact
      721 tgcaacggga gttgctctta ctacacttgc tgcttgctct ggaggttcaa attctacgac
      781 tgcttcttca tctgaagaaa aagctgataa aagtcaagaa ttagttatct attcgaactc
      841 agtctcaaat ggtcgtggtg attggttaac tgctaaagca aaagaagctg gttttaatat
      901 aaaaatggtt gatatcgctg gcgctcaatt agcagaccgt gttattgctg agaagaataa
      961 tgcagttgca gatatggtat ttggaattgg tgctgttgac tcaaataaaa ttagagatca
     1021 aaaattacta gtacagtaca agcctaaatg gttagataaa attgatcaat ctttatcaga
     1081 taaagataat tattataatc ctgtgattgt tcaaccatta gttttaattg gggcgcctga
     1141 tgtaaaagaa atgcctaaag attggactga attaggtagt aagtataaag gtaaatattc
     1201 aatttatggt cttcaaggtg gtacaggacg ggcaattcta gcaagtatct tagttcgtta
     1261 ccttgatgaa aaaggagatt taggcgtatc agaaaaagga tgggaagttg caaaagaata
     1321 ctttgcaaat gcctatactc ttcaaaaagg tgaaagctca gttgttaaag cgttagataa
     1381 agaaagccca attcaatacg gtatgatctg gggttctgga gcgttaattg gacaaaaaga
     1441 acaaaatgtt gaattcaaag ttatgactcc tgagattggt gtaccatttg taactgaaca
     1501 aactatggtt ttaagcacta gtaaaaaaca agcgttagct aaagaattta ttgattggtt
     1561 tggtcaatca gaaattcaag tagaatatag taaaaaattt ggatctattc ctgcaaataa
     1621 agatgcccta aaagagttac ctgaagatac gaaaaaattt gttgatcaag tgaaaccaca
     1681 aaatattgac tgggaagctg ttggaaagca tttggatgaa tgggtagaaa aagctgaatt
     1741 agaatacgta aaataagaaa taagagttat tttttatgtc caagtctctc tattatttct
     1801 aaatttaagg aagaaataaa gagaggcttt tggttttagt aatagaggga gttagtgaga
     1861 tgattaaatt tgataatatt caaattaaat atggtgattt tgttgcaatt gataatctga
     1921 atttagatat acatgaaggg gaattcttta catttcttgg gccttcagga tgtggtaaat
     1981 caactacttt gagagcattg gtaggttttt tagatccatc atcaggaagt attgaagtta
     2041 atggaacaga tgtcactcat ttggaacctg aaaagcgtgg aattggtatt gtatttcaat
     2101 cttatgcgct atttccaact atgactgttt ttgataatat tgcatttggt ttaaaagtta
     2161 agaaggtagc tccagatgtt attaaagcta aagtatcagc agtggcagca aaaattaaga
     2221 tctctgatca acagttacag cgtaatgtat cagaattatc tgggggtcaa caacaaaggg
     2281 tagcattggc tcgtgctctg gttcttgaac ctaaaattct ttgtctagat gaaccattgt
     2341 caaaccttga cgcaaaatta cgtgtagatt tgagaaaaga gttgaaaaga cttcaaaaag
     2401 agttaggtat tactacttta tatgttactc atgatcaaga ggaagccttg actttatctg
     2461 atagaattgc agtctttaac aatggataca tcgaacaggt cggtacacca gtagagattt
     2521 atcataattc tcaaactgaa tttgtatgtg attttattgg agatattaat gttttgaccg
     2581 atgaaacagt ccacgaagta ttactgaaaa atacaagcgt tttcttagaa gataaaaaag
     2641 gttacattcg attagagaaa gttcgattca atcgtgaaac taaacaagat tttattctaa
     2701 aagggacaat tattgatgtt gagttttctg gagttacaat tcactataca ataaaagttt
     2761 ctgaaagtca gattcttaat gtaacaagta ttgatagtca ggctgctatt agatctgtcg
     2821 gagaaagtgt ggaattattt atcacaccat cagacgttct gcaattttaa ggaggtgcag
     2881 tatgcgtcat aaattaaatt taaaagattg gcttattcgt ttagggttaa tctggttctt
     2941 agtaacattt attatttatc caaactttga tctagtagtg aatgtatttg taaaaggagg
     3001 agaattttcc cttgatgctg tacatcgtgt tctaaaatct cagagggcac ttcagagtat
     3061 tatgaacagt tttaagttag cattttcact cattattaca gttaatgtcg taggtattct
     3121 ttgtgttcta tttacagagt actttgatat taaaggtgct aaaattttaa aattaggtta
     3181 tatgacctct ttaatttatg gaggagtggt tttagcgact ggatataaat ttgtctatgg
     3241 tccttatgga ttgattacaa aatttttaca aaatgttatc ccttctttag actctaactg
     3301 gtttattggg tatggtgcag tcttattcat tatgacattt tcaggaactg ctaatcatac
     3361 attgttttta acaaatacaa ttcgaagcgt tgactatcac actattgagg ctgctcgaaa
     3421 tatgggagca aaaccattta ctgttttccg aaaagtagtg ttaccaacct taattccaac
     3481 tctatttgca cttactatta tggtttttct tagtggttta tctgcagtag cagcacccat
     3541 gattgttggt ggtaaagaat ttcaaactat aaatccaatg attattacat ttgcagggat
     3601 ggggaattct cgtgatttag ctgccttact tgcaattatt ttaggtattg caactacaat
     3661 tttgcttact attatgaata agatagaaaa aggtggaaat tatatttcta tctctaagac
     3721 taaagcgcct cttaaaaaac aaaaaattgc gtctaagcct tggaatatca ttgctcacat
     3781 tgtagcatat ggattgttca cagttttcat gcttccacta atttttatag tattatactc
     3841 atttacagat ccagttgcaa ttcaaacagg taacttaaca ttatcaaact ttactttaga
     3901 aaattatcgc ttattcttta gtaatagtgc ggcattctct ccattcttgg tcagctttat
     3961 ttattctatt attgctgcga caacagcaac aattctcgca gttgtatttg ctcgtgttgt
     4021 cagaaaacat aaatctcgtt ttgatttctt atttgaatat ggtgctctac ttccttggtt
     4081 actaccaagt acacttttag cagtaagttt attatttact tttaatcagc cacaatttct
     4141 tgtcttgaat cagattttgg taggtagttt ggtaattcta cttattgcat atatagttgt
     4201 aaaaatccca ttttcttata gaatggtacg tgctatttta tttagtgttg atgatgagat
     4261 ggaagatgca gcaagaagta tgggtgcttc acctttttat actatgatga aggttatcat
     4321 tccatttatt ttaccagttg ttctctctgt tattgcttta aactttaact ctttattaac
     4381 tgacttcgac ttatctgtat tcctttacca tcccctagct caaccattag gtattacgat
     4441 tcgatctgca ggtgatgaaa cagcaacatc taatgcacaa gctctggtat ttgtttatac
     4501 aattgttctg atgattattt ctggaacggt attatacttc acacaaagac cggggcgtaa
     4561 agtaaggaaa taatcatgac agccactagt cttgggttat caaatattga aatagttgtc
     4621 aggattgttt tatcagtagt cattggtagt ataattggtt tagagagagg gagcaaatcc
     4681 cagcctgcag gcatccgaac ttatagtatt gtttgtctag ctgcatgttt gattatgatg
     4741 acgaatgaat acgtatctta taaatttggg acaggagatc ctacacgatt aggagctcaa
     4801 gttatatcag gtgtgggttt tctaggcgct ggaacgattc ttattacaga taaaaagaaa
     4861 attacaggtc tgacaactgc agcaggcatt tgggcttcgg caggaattgg attagctatt
     4921 ggagtaggtt tttatgaggg agctctttta gtagccattt ctgtttgggg tgtgatatcc
     4981 atgttccaac cactaaaaaa atatctgcaa aatcgttcta aaatgattga attgtatata
     5041 gtggttaaat ctacagaagc ctataatcgt gtattggttt attgtgcaga aaatggtatc
     5101 agaatgaccg attcaagaac agcgtttgga gatgttaatt ctgacagaat tgaatacttt
     5161 gatgttccgg acaaaaaaat agcgtcattt attactctgg aattatcagg tagatttgaa
     5221 catcttcgac tgatggaaga aattgctaat attgtaggtg ttatttatgt cgaagaaatt
     5281 agctgaaatt tagtaaacaa agggacagat gtttttgtcc ttttgttttt tagatgtttt
     5341 aatcatctaa tttttgagta gatttttgct cagaggtttt tatgaaagat
//
LOCUS       DAVKZB010000108         5130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKZB010000108 DAVKZB010000000
VERSION     DAVKZB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.108"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(177..1739)
                     /gene="guaA"
                     /locus_tag="VXA80_001717"
     CDS             complement(177..1739)
                     /gene="guaA"
                     /locus_tag="VXA80_001717"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV0354174.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1881..2579
                     /locus_tag="VXA80_001718"
     CDS             1881..2579
                     /locus_tag="VXA80_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0354175.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2622..3518)
                     /locus_tag="VXA80_001719"
     CDS             complement(2622..3518)
                     /locus_tag="VXA80_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0354176.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3527..4462)
                     /locus_tag="VXA80_001720"
     CDS             complement(3527..4462)
                     /locus_tag="VXA80_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV0354177.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4459..>5130)
                     /locus_tag="VXA80_001721"
     CDS             complement(4459..>5130)
                     /locus_tag="VXA80_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEV0354178.1"
                     /translation="KLNETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRT
                     RKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHA
                     EDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASLDFIPA
                     QGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLGKDRNNVEL
                     WFEEV"
BASE COUNT         1601 a         1119 c          955 g         1455 t
ORIGIN      
        1 tatatacaaa aaactctctt agtacatatt cacactaaga gagttttttg taatctattt
       61 gccaagtttg gcgggaaaat tggcggggta acttttctaa aaagataaat ttataccatt
      121 ctaacttcta taaacccgat agaatcggtg taaaacattc atttttactt tctatattat
      181 tcccactcaa ctgttgcagg tggtttactt gtaatatcgt agacgatacg gttaacatga
      241 tccacttcat ttacgatacg tactgagatt ttttgaagta cttcccatgg aattttggca
      301 aaatcagcag tcataccatc gatagaagtg atagcacgga ttgcaatcgt gtagtcatac
      361 gtacgaccgt cacccataac accgactgaa cgaacgcctg tgttaacagt gaagtattgc
      421 caaatatcgc ggtcaagtcc agctttagcg atttcttcac gaagaatagc gtctgattca
      481 cgaacggttt caagtttctc ttcagtgatt tcacccatga cacggatagc aagtcctggt
      541 cctgggaatg gttggcgcca tacgatatgg tctggcatac caagctctgt accaagagca
      601 cgaacttcat ccttgtaaag agtattgagt ggttcaatca attcaaactg catatcttct
      661 ggaagaccac ccacgttgtg gtgtgacttg atagtttgag ctgtatccgt accagactcg
      721 ataacatctg tatataaagt accttgagca aggaatttca catctttgag cttgcttgct
      781 tcgtcatcga atacatagac aaactcgtta ccgatgattt tacgtttttg ttcaggatca
      841 gaaacgccag caagtttgtc aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa
      901 ccaaacttac caccgagcat gtccataact tgatcagctt cgcctttacg aagaagaccg
      961 tggtctacga agatacagat caattgatcg ccaatcgctt tttggagaag aaccccaacg
     1021 acagatgagt caacaccacc tgatagacca agaaggacac gtttatcacc gacggtttca
     1081 cgaatttttt tgatctgcat gtcaatgaaa ttatccattg accagtcacc tttagcctta
     1141 caaatgttaa gggcaaagtt acgaaggata tcatttccgt atacagaatg acgaacttct
     1201 gggtggaatt ggataccgta aatgtgttta tctgggtttt cgatggctgc gtatgggcag
     1261 tcagctgatg tacctgtacg aacaaagtca gcaggaatct cagtaaccgc atcaccatgg
     1321 ctcatcaaaa cagtctgttc atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt
     1381 agggttgatt gaccgtattc acgatttcca gcatcacctg caggaacaac ttttcctcca
     1441 agtttatggg tcaataactg cataccataa cagattccca aaattggaat tccgagttcg
     1501 aagatttctg ggtcaatatc aaatgaacca tcttcatata cagaatttgg accacctgat
     1561 agaataattc ctacaggatt gacttcacga acttcagcag ctgaaatttt atggcttttt
     1621 agttctgaaa aaacaccaat ctcacggata cggcgtgaaa tcagctggtt gtactggcta
     1681 ccatagtcca atacgatgat tttttctaca tcttgcaaat cagttgaaat gttgctcatc
     1741 tttttccttt ctcaaaagaa atatcttttc tcaatattct atcacaaata agggcgaatt
     1801 accaactaaa caaggcgaag tttgactttt tctagtttat tggggtacaa tagagaaaag
     1861 atagaaatga agtgaaaaat atgctacctg cttatatgaa aatccatgat cagataaaaa
     1921 aggatattga cgagcaccgt tgggctattg gtgagaggct tcccagtgaa agagatttag
     1981 ctgagcagtt tgcggtcagt cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag
     2041 aaggcgtctt agagcgccgt gtaggaagcg gcacctttgt ttccagtact cgagtacaag
     2101 aaaagatgcg agggacaacc agttttactg aaattgtcaa atcccaaggt aaagttccct
     2161 ctagccagct catttcctac aaaaaaacca ttcccaatga gcaagaagtt gccaagctag
     2221 gaatttttcc aacggacaat attatccgaa tggagcgggt ccgctatgcc gaccaagttc
     2281 ccctagttta tgaagttgct tctattcctg aaaaattcat taaggacttt aaaaaagaag
     2341 aaatcaccag tcatttcttc caaaccttgc aaaaacatgg ctatcgtatc ggtaaatccc
     2401 aacagaccat ctatgctcgc cttgctaaag aaaagattgc ccactatttg gaagttgaaa
     2461 aaggacatgc tattcttgga ttgacccaga tttcctacct agaagatggt actgcttttg
     2521 aatacgtaaa aagtcaatat gtaggcgaac gctttgaatt ttatcttgaa aataattaga
     2581 ttaccacgca aaaactctct gtcacggaca aagagtcttt tttatttttc taagaataaa
     2641 tctttcaaag aaatcaaagt tacagcaaac aatatcattg ccataccaag aaaatcaatg
     2701 ggataaaatt gttcattcat tattaagaag gcaaagaaaa tcgccgatat tggctcaatt
     2761 gaagccaaca agcttgactt gaccggtcct atcagactgg ctcctttaag gaaagctgta
     2821 taggcaaaga cagtcccgat aaggataatg cctgcaaacg caaggagaaa atcaagacta
     2881 gtcgggatat cggcctgtag aacccctgta aaaggaaggg cgaccaaacc tgctatgacc
     2941 attcccacac caatgaccaa gctgctcccc cactttttaa tcaaggctat gggtaaaatg
     3001 atatacagag cataagtcaa ggcagaaaag agaccccaga acagaccagc aggtgtcatg
     3061 gataactggt ccaactgccc atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg
     3121 atggaaacta tctctcccag tgtcggtgcc accctatcct tgatacagct ataaattaaa
     3181 attccgacag gacaaacata ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata
     3241 gcagacagat aggcgaattg gttgagaaaa agaccaatca gagcaaaaat aagaagagac
     3301 agcaaactct ttctatcctt taaaaagacc agtattttat cctttgcagt agcataagcc
     3361 aagagcatga gaattccacc agcgattaaa agacgcaagt tagtcaagac cagagccgaa
     3421 attccgtgtg ccattaggta ttggccactc gttcctgaca acccccaagc aataccagcc
     3481 acaactgtta gtaaagtccc ttttaaacta tttgacatgt ctctccttac tcttctttgc
     3541 gaatcaagtt catcacttga ttgcggtcta atatccattg agctctctcg tccttttcat
     3601 aggtacgatt cgataggaaa atagtggctt cttgcttctg acgattccac atgataaagg
     3661 tacctgtata gcccgtatgg tctagccaat ctccttccaa attccatgct aaagaacgtt
     3721 ccttgtcatc caaaggagaa aaattttgat ttaagtctct tgcaaaatca tctgctaaat
     3781 agtgttctaa aaagatttgt aaatccttta tagtcgaaaa taaaccagca ctcccagcat
     3841 gtctacccag gagacgagcc ttgggatcat gcactatgcc tgcctctaca cctctaactg
     3901 ttggaacagc aagctcaact ggcccaaact tggtttccgt cattccccaa ggtttccaga
     3961 cttgatcctt taaaatcaca tccaaatctt gattaaaaat tctttccaaa ataaagccca
     4021 acagcaaaaa atggacatcc gaataaagaa aggctggctg acttcgtctg ttgagatgaa
     4081 acatcgcttc ctttaattca ggggctgtta aaagatcacg attaggaata aaaggatcaa
     4141 ggtctgttgc atgagtcaag agctggcgaa tagtgatgtc tggataatca ctctcaggta
     4201 aaaaatctat taccagtcta tcaatatcta attgacctat ttcccacaag aaggtacaaa
     4261 ctgtgccaac cccaacaacc ttgctgacac tagctaggtc ataaactagt cctgcctcag
     4321 tctgcaagcc atgctctggg tcactctggc ctaaatagaa ctctgtccat tgattgtcct
     4381 taaaatacgc aaaagaggct ccgggataaa tccctgcctc gatttgttct tctatttttt
     4441 taataatctt ggtccacttc atacttcttc aaaccacaat tcaacattat ttctatcttt
     4501 accaaggaag aatttttcag acttaggaat aaaatattca gtagactcaa acttctggcg
     4561 aagacttgct atgtctaatt cattgaccaa gaacttgagc atagataagt cccaggtaac
     4621 cgtattgtcc acagtcaaat cctgcccctg agctggaata aaatcaaggg atgccccaat
     4681 ttcagatgat tccaagaaac tttcgatatc agttgggaga tgtaattcca tagaaatctc
     4741 aaatttactt aaagaaattg atgccaaatc tgtttgaaat tcaggctttt ctcctacttc
     4801 tactagactt gctatgtcat cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa
     4861 aatttcaaaa gcgtagccat tttgaccttt atataatcga tgaatcgaat ctgttttaga
     4921 taagattcct tcaatttcta agggattttc caccttgaca atcaatctag ctagtttttt
     4981 tcttccctct accttacgag tacgcatact gggagcttct tctaaaacca gcttttcaag
     5041 acccgtttgg tcacctagtg acagaaaggc cgattcttct aacaaggcct tcattccaag
     5101 ggtttcaata taaaatgttt catttaattt
//
LOCUS       DAVKZB010000109         5103 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKZB010000109 DAVKZB010000000
VERSION     DAVKZB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.109"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            434..886
                     /locus_tag="VXA80_001722"
     CDS             434..886
                     /locus_tag="VXA80_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0354179.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VXA80_001723"
     CDS             905..2242
                     /locus_tag="VXA80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0354180.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VXA80_001724"
     CDS             2321..3205
                     /locus_tag="VXA80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0354181.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VXA80_001725"
     CDS             3205..4038
                     /locus_tag="VXA80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0354182.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGVNKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4056..>5103
                     /gene="nanB"
                     /locus_tag="VXA80_001726"
     CDS             4056..>5103
                     /gene="nanB"
                     /locus_tag="VXA80_001726"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV0354183.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKADFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAG"
BASE COUNT         1678 a          805 c         1009 g         1611 t
ORIGIN      
        1 ttagaaaaac gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatactgaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgctttgagg ttgtagataa gactgacgta tataatccaa ggtgaagcga
      301 ctgtgctttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgttcttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccgttta ggaacttatg tgggagtcaa
      481 tccacatttt gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggggctttct ttgaaacaca ccaaaaatat ttggatattc atttagtttt
      661 ggaaaacgaa gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata
      721 tgatgaagag aaagatattg aattatacac agggaaagtg gaacagttgg ttcatttgag
      781 agctggtgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagacg ctttggttcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaact ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaaat ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt taactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaacga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgagctga
     2281 aaagctctag cccaatcttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttctct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gctggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtattgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcagttt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca accctcattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct atattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgt aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata agattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa
     4081 aactaggaat ttctgttgta ggcattagtc ttttaatggg agttcccact ttgattcatg
     4141 cgaatgaatt aaactatggt caactgtcca tatctcctat ttttcaagga ggttcatatc
     4201 aactgaacaa taagagtata gatatcagct ctttgttatt agataaattg tctggagaga
     4261 gtcagacagt agtaatgaaa tttaaagcag atgaaccaaa ctctcttcaa gctttgtttg
     4321 gcctatctaa tagtaaagca gactttaaaa ataattactt ttcaattttc atgagagatt
     4381 ctggtgagat aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcca
     4441 gaccagcttc attatgggga aaacataaag gacaggcagt tgaaaataca ctagtatttg
     4501 tatctgattc taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg
     4561 aaacagttga tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag
     4621 gagctgttaa tcgtgaaggt aaggaacatt acctcgcaaa aggaagtatt gatgaaatca
     4681 gtctatttaa caaagcaatt agtgatcagg aagtttcaaa tattcccttg tcaaatccat
     4741 ttcagttaat tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac
     4801 tatatacatt aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc
     4861 atgattctaa aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt
     4921 ggagtgagcc aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc
     4981 cacgagataa taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat
     5041 ccattgttga agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg
     5101 gta
//
LOCUS       DAVKZB010000110         4854 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKZB010000110 DAVKZB010000000
VERSION     DAVKZB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.110"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(56..1066)
                     /locus_tag="VXA80_001727"
     CDS             complement(56..1066)
                     /locus_tag="VXA80_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV0354184.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLTTLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VXA80_001728"
     CDS             1215..2384
                     /locus_tag="VXA80_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0354185.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VXA80_001729"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VXA80_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV0354186.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDASLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VXA80_001730"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VXA80_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV0354187.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQSLSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VXA80_001731"
     CDS             4146..4379
                     /locus_tag="VXA80_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0354188.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4425..4715
                     /locus_tag="VXA80_001732"
                     /pseudo
     CDS             4425..4715
                     /locus_tag="VXA80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1450 a          909 c         1217 g         1278 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggagatggct tgggctactt ccttttcctt agcatggata acaggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaatcaag gtagtcagtt
      721 ttgtatccag ggcaaagaag cgctggaaga ggtaggcaac aatcaaggct attgaaatgg
      781 tggaaaggat gacagggagt gaagattgcc caactgcgaa gacttgagag atattgagac
      841 caaaaccaag taagataacg gcatactgga gcaatttctt ggaactaaag gtcaatccag
      901 catccagttg tttataagac gagagaaagg gatgtaggag catgcccata aaaatcgcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcagac caagatgcct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 agaatctgtt accattatag acctgtaaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcgggaggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggcta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga agtttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctccg gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccagcg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttac aggacaatca
     2701 gcaggatgct tccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gatgagagac gttgccatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaactata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat cgttgagggt gtcaatcaat ccctatctga cttttcagat
     3241 atcgaggttc aactctacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attcggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgctttgt tggctgctgg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggacttatgt caacattgcc gactatagat
     3541 gggaaaggtt ttgacatgct tgacctcggt gctaatgcag aaaatacagc ccagcacctc
     3601 catcaatacg ctgtcctagg ttctttctat gccaagaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga cgtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaactccgag gaaatcaagt aaacttaagc cattaactct tgaagacaaa gcctgtaacc
     4561 atgcactatc taaggagagc agcaaggttg agaatatttt tgctaaagta aaaacgttta
     4621 aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga
     4681 ttgctggcat tatcaaccgt gaactaggat tctagttttg caggaagtct aacagtaaaa
     4741 aagtgattag aaaacatctt ttttaaaaat agagatgatt ttgaaacaaa aaagctaatt
     4801 caagacgttt cgatgccaat tcaagatttg gatgaaaaaa attaatagat actg
//
LOCUS       DAVKZB010000111         4645 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKZB010000111 DAVKZB010000000
VERSION     DAVKZB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.111"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..1323
                     /locus_tag="VXA80_001733"
     rRNA            <1..1323
                     /locus_tag="VXA80_001733"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1375..1447
                     /locus_tag="VXA80_001734"
     tRNA            1375..1447
                     /locus_tag="VXA80_001734"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:Ala,seq:tgc)
     gene            1572..4473
                     /locus_tag="VXA80_001735"
     rRNA            1572..4473
                     /locus_tag="VXA80_001735"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4550..>4645
                     /gene="rrf"
                     /locus_tag="VXA80_001736"
     rRNA            4550..>4645
                     /gene="rrf"
                     /locus_tag="VXA80_001736"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1287 a          959 c         1378 g         1021 t
ORIGIN      
        1 actaccagat ggacctgcgt tgtattagct agttggtggg gtaacggctc accaaggcga
       61 cgatacatag ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac
      121 tcctacggga ggcagcagta gggaatcttc ggcaatggac ggaagtctga ccgagcaacg
      181 ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa gaacgagtgt
      241 gagagtggaa agttcacact gtgacggtat cttaccagaa agggacggct aactacgtgc
      301 cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg atttattggg cgtaaagcga
      361 gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc ttaaccatag taggctttgg
      421 aaactgttta acttgagtgc aagaggggag agtggaattc catgtgtagc ggtgaaatgc
      481 gtagatatat ggaggaacac cggtggcgaa agcggctctc tggcttgtaa ctgacgctga
      541 ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ctgtaaacga
      601 tgagtgctag gtgttagacc ctttccgggg tttagtgccg tagctaacgc attaagcact
      661 ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa
      721 gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc
      781 cctctgaccg ctctagagat agagctttcc ttcgggacag aggtgacagg tggtgcatgg
      841 ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccctat
      901 tgttagttgc catcatttag ttgggcactc tagcgagact gccggtaata aaccggagga
      961 aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa
     1021 tggctggtac aacgagtcgc aagccggtga cggcaagcta atctcttaaa gccagtctca
     1081 gttcggattg taggctgcaa ctcgcctaca tgaagtcgga atcgctagta atcgcggatc
     1141 agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag
     1201 tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa ggtgggatag
     1261 atgattgggg tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg gatcacctcc
     1321 tttctaagga taaggaactg cgcattggtc ttgtttagtc ttgagaggtc ttgtggggcc
     1381 ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta
     1441 ggctccattg gtgagagatc accaagtaat gcacattgaa aattgaatat ctatatcaaa
     1501 tagtaacaag aaaataaacc gaaaacgctg tagtattaat aagagtttat gactgaaagg
     1561 tcagaaaata aggttaagtt aataagggcg cacggtggat gccttggcac taggagccga
     1621 cgaaggacgt gacaaacgac gatatgcctt gggtagctgt aagtaagcga tgatccaggg
     1681 atttccgaat gggggaaccc aacaggtagt acctgttacc cacatctgtt aaggatgtga
     1741 ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa gcaaaagcga
     1801 ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt actcttcggg
     1861 gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga agatcagcca
     1921 aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc ctgagtacgg
     1981 cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct aaatactccc
     2041 tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc gggaggggag
     2101 tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat gggtgagagc
     2161 gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag ttgaagagac
     2221 ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt agacccgaaa
     2281 ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga ggaccgaacc
     2341 agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc aaacgaactt
     2401 ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta gagattcttg
     2461 gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca gataaactcc
     2521 gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg tagtcgaaag
     2581 ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga aaaggatgtg
     2641 gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa agagtgcgta
     2701 atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg
     2761 aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat gaaggtatac
     2821 cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg aaagacaggt
     2881 gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg ccctgggtta
     2941 gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg atattcctgt
     3001 actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg attggaagag
     3061 tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac attgagctgt
     3121 gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga aaagcttcta
     3181 gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg cgagtagcct
     3241 caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag
     3301 aaggggtgct gacttaaagt cagccgcagt gaataggccc aagcaactgt ttatcaaaaa
     3361 cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc cggtgctgga
     3421 aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag taaacggcgg
     3481 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac
     3541 gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg
     3601 tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta ctgcagtttg
     3661 atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat cgggacgcca
     3721 gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct aacccagata
     3781 ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa
     3841 aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc gcagagtgta
     3901 aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa agtcgggctt
     3961 agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc taccctgggg
     4021 ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca cctcgatgtc
     4081 ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag
     4141 cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg
     4201 taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact taccgctggt
     4261 gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg ataaacgctg
     4321 aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat atatcagtaa
     4381 gagccctgag agatgatcag gtagataggt tagaagtgga agtgtggcga cacatgtagc
     4441 ggactaatac taatagctcg aggacttatc caaagtaact gagaatatga aagcgaacgg
     4501 ttttcttgaa ttgaatagat attcaatttt gagtaggtat tactcagagt taagtgacga
     4561 tagcctagga gatacacctg tacccatgcc gaacacagaa gttaagccct agaacgccgg
     4621 aagtagttgg gggttgcccc ctgtg
//
LOCUS       DAVKZB010000112         4594 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKZB010000112 DAVKZB010000000
VERSION     DAVKZB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.112"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            22..726
                     /locus_tag="VXA80_001737"
     CDS             22..726
                     /locus_tag="VXA80_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV0354189.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    785..872
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            908..1222
                     /gene="rplU"
                     /locus_tag="VXA80_001738"
     CDS             908..1222
                     /gene="rplU"
                     /locus_tag="VXA80_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV0354190.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1238..1582
                     /locus_tag="VXA80_001739"
     CDS             1238..1582
                     /locus_tag="VXA80_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV0354191.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1599..1892
                     /gene="rpmA"
                     /locus_tag="VXA80_001740"
     CDS             1599..1892
                     /gene="rpmA"
                     /locus_tag="VXA80_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV0354192.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2097..2302)
                     /locus_tag="VXA80_001741"
                     /pseudo
     CDS             complement(2097..2302)
                     /locus_tag="VXA80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2739..3656
                     /locus_tag="VXA80_001742"
     CDS             2739..3656
                     /locus_tag="VXA80_001742"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV0354193.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVEQLKADEEVTRNWS"
     gene            complement(3749..4594)
                     /locus_tag="VXA80_001743"
     CDS             complement(3749..4594)
                     /locus_tag="VXA80_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0354194.1"
                     /translation="MKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITFY
                     LFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQG
                     DLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCILM
                     LILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDLG
                     RGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEGF
                     TFEKE"
BASE COUNT         1457 a          782 c          949 g         1406 t
ORIGIN      
        1 atatttctaa gagaaaatac aatgggaggt aaaatgaggt tattacctat aagaaaaata
       61 tcacgtcagt ctaaaaggtt agcacttttt ttgacgtttt gtgctggata tgtggatgct
      121 tacactttta ttgttcgcgg gaataccctt gtagctggac aaactggaaa tgttgtcttt
      181 ctttcagtag aattaattaa aaataatgtt tcggatgtta gggacaaggt tctcaccttg
      241 ctagcgttta tgatgggagt ctttttatta acgatttata aggaaaaatt gagaattgtg
      301 aaaaaaccta ttctgtcctt gattcccttg gcaatcttat caatcattat tgcttttgtg
      361 ccgcaaactg tggataatat ctatctagtg ccgcccttgg ccttctgtat gggactggtg
      421 acaactgctt ttggagaagt gtcgggtatt gcctataata acgcttttat gacagggaat
      481 atcaaacgga ccatgctggc ttttggagat tatttccgaa ccaagcacac tccttttttg
      541 cgtgaaggat tcatatttgt tagcctgctt agtagttttg tccttggcgt tgtcttttca
      601 gcctatttga cgattttcta tcatgaaaag accattcttg gtgttcctat tatgatgagc
      661 gttttttacc tcagcatgct ttttgcctct tggcagaaaa aagtaaaaga aaaagcttca
      721 ttttaggtgt tattacttgt aagaaaatag gagatatgct atacttgaag agttgactat
      781 gcacgatcct gtgcaaccgc acgaacatcg ttgcttgatt attcaagttt aagtggttcg
      841 tcccacgatg ctaggcgagt cttcacaaaa aatcggggaa acccataaaa atcataggag
      901 gtgcataatg agcacatacg caattatcaa aactggcgga aaacaagtta aagttgaagt
      961 tggtcaagca gtttacgttg aaaaattgaa cgttgaagct ggtcaagaag ttacttttaa
     1021 cgaagttgtt cttgttggtg gtgaaaacac tgttgtcgga actccacttg ttgctggagc
     1081 tactgtagtt ggaactgttg aaaaacaagg aaaacaaaag aaagtggtta cttacaagta
     1141 caaacctaaa aaaggtagcc accgtaaaca aggtcaccgt caaccatata caaaagttgt
     1201 catcaacgca atcaacgctt aattttaagg agaacacatg atacaggcag tctttgagag
     1261 agccgaagat ggcgagctga ggagtgcgga aattactgga cacgccgaga gtggcgaata
     1321 cggcttagat gtcgtgtgtg catcggtttc tacgcttgcc attaacttta tcaattctat
     1381 tgagaaattt gcaggctatg aaccaatcct agaattaaac gaagatgaag gtggctatct
     1441 gatggttgaa ataccaaaag atcttccttc acaccagaga gaaatgaccc agttattctt
     1501 tgaatcattt ttcttaggta tggcaaactt atcggagaac tattctgagt tcgtccaaac
     1561 cagagttatc acagaaaact aacacggagg aaaacattat gttaaaaatg actcttaaca
     1621 acttgcaact tttcgcccac aaaaaaggtg gaggttctac atcaaacgga cgtgattcac
     1681 aagcaaaacg tcttggagct aaagcagctg acggacaaac tgtaacaggt ggatcaatcc
     1741 tttaccgtca acgtggtaca cacatctatc caggtgtaaa cgttggtcgt ggtggagacg
     1801 atactttgtt cgctaaagtt gaaggcgtag tacgctttga acgtaaagga cgcgataaaa
     1861 aacaagtttc tgtttaccca atcgctaaat aaaaaggtcc attgaacctt ttatcccgaa
     1921 ccttgaaatg taaaggtgag gaagctagaa acagcttaaa ataaggcttt ccgagttttc
     1981 ggagagcctg tttttttgtt ttggtaccca tactttttaa tatgtatagt ggattgaatc
     2041 tggaatagaa cactgttaaa aattgatatg agtttcctac tcgatttctt cagattttat
     2101 ttcaattgac tataagaact gctatctggt ttcaaatatt ggcttgtcgt cccttcactt
     2161 gtctgaatgg tttgagccgt tccttctgat aagggaaaag atattcttta gctgaaacgg
     2221 tctgagaatc caagctaaca gatggagaga aaaatagcct agtctgaagc acactagatg
     2281 ggagttgttg gaaagcagtc atctagtttt tctttgctgt tttggctttt ttctttgtca
     2341 tcagggaaag tgacttgctt caaatcaggg agtttaggaa attcttcaaa catcttgccc
     2401 aagttgtctc attttgtaaa ttttacagcc ataggtgtac ctcgattcta aattctacct
     2461 tttcggcacc aattagtaat ttaaagttct caggagttag ttgcattgct ttttcataca
     2521 taagcatagt atgacacttt atggttaccg aacaagtcta atcactcctt gcaaaatttt
     2581 gaagatgtcc tacaagtttt tgagaagaat attgcaaagt tcatagttaa gcaacgatag
     2641 aataaagtgc tttttgaaaa cagtacactt catgtgaaaa aaacaacatt tcagattttc
     2701 tgttccagaa atagctatcg ctatgatata attattttat gattattact attcctataa
     2761 aaaaccaaaa agatattggc acaccatctg attcagtcgt tgttctcggc tattttgatg
     2821 gcatacataa ggggcatcaa gaattatttc gtgttgccaa caaggctgcg agaaaggatt
     2881 tattacctat cgtcgttatg acctttaatg aatctccaaa gatcgcttta gagccttatc
     2941 atccagattt gtttttgcat attttgaacc ctgctgaacg tgaaagaaaa ttaaagcgcg
     3001 aaggtgtaga agaattatat ctccttgatt ttagtagtca attcgctagt ctcactgcac
     3061 aagaattctt tgcaacttat atcaaggcta tgaatgccaa aattattgtt gcaggttttg
     3121 attatacatt tggttctgac aaaaaaacag cagaagattt aaaggattac tttgatggag
     3181 aagttatcat tgttccacct gtagaagatg agaaaggaaa gattagttca actcgtatcc
     3241 gtcaagctat tttagatgga aatgtgaaag aagcaggaaa acttttgggg gcaccgcttc
     3301 catcaagagg tatggtggtt catggtaatg ctcgtggtcg tacaattggt tatccgacag
     3361 cgaatttagt gcttttagac cgtacttata tgccagcaga tggcgtttat gtcgttgatg
     3421 ttgagattca aagacagaag tatcgtgcta tggctagtgt cgggaaaaat gtgacctttg
     3481 atggagaaga agcacgtttt gaagtcaata tttttgattt taatcaagat atttatgggg
     3541 aaaccgtcat ggtttattgg cttgatcgca ttcgtgatat gaccaaattt gactcagttg
     3601 accaattagt ggaacagtta aaggctgatg aagaagtaac tcggaattgg tcttaagagc
     3661 ttgagtaaat aaaacaaaaa agaggttgtc tgtaacccaa aagatagatg atttagtcta
     3721 acttttgagg tcacgacatt acctcttttt attctttttc aaaggtgaag ccttctccta
     3781 ggatttcatg ggcttctgta atagttataa aggcttgagg atcgattcga tgaatcattt
     3841 ccttcgtttt cacaatttca tttcttccga caatacagta gatgattttc aaattttctt
     3901 tactatagta gccttgacca gaaataaaag taacacctct tccgaggtca tcattaatcg
     3961 ccttagcaag ttggtcagga cgttttgtga taatcataaa gcctttgccg gcatatcctc
     4021 cttcaccaat caaatcaata acacgagaaa caataaaatc aaacaaaagc gtgtaggaaa
     4081 ccaatctcaa atccttgaag attaggagaa tcaacatgag aatacaaaaa tctaagataa
     4141 agagcagttt tcctatggat atatgagtgt atttgttgag aatacgagct agaatatcag
     4201 ttccgccagt tgtacctcca gcattaaaaa taattccaag gccaattccc aataggattc
     4261 ccgctataag ggctgtgatt agtaaatcac cttgaagatc aatatgaagg ggaatatgct
     4321 caaaaaaagc taaccaggcg gacaaagcta aggttcctag taaactagaa tagagggatt
     4381 tggctccaaa gatcttccaa gctaggatga aaaggggaat attaatcagc aggttcatga
     4441 gggaaacagg gattttaaaa agataaaagg tgatgagggt aatgcctgtc gcccctcctt
     4501 caaagagatg atggggaact acaaaataag tcagtccaaa agcataaata gcagcaccta
     4561 gtaaaatggt aaaaatgggg taaatttttt taat
//
LOCUS       DAVKZB010000113         4591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKZB010000113 DAVKZB010000000
VERSION     DAVKZB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.113"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            127..885
                     /locus_tag="VXA80_001744"
     CDS             127..885
                     /locus_tag="VXA80_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0354195.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTLNGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAILSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            925..1689
                     /locus_tag="VXA80_001745"
     CDS             925..1689
                     /locus_tag="VXA80_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0354196.1"
                     /translation="MISLFGLAAAKPVQADTNIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1702..2379
                     /locus_tag="VXA80_001746"
     CDS             1702..2379
                     /locus_tag="VXA80_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0354197.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2389..3048
                     /locus_tag="VXA80_001747"
     CDS             2389..3048
                     /locus_tag="VXA80_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0354198.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3297..4487
                     /locus_tag="VXA80_001748"
     CDS             3297..4487
                     /locus_tag="VXA80_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV0354199.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         1295 a         1075 c          827 g         1394 t
ORIGIN      
        1 ttgactgtcc cgatcgattt gtcctgttct tatttcacta tagtgaattt tttcagatat
       61 ggtttgacaa gaacattctt ttattgtaga atcgtgttat aaaatctaac ataaatgaga
      121 tctcccatgg ctttagtaga atttaaaaac gtcgaaaaat attacggaga ctaccacgca
      181 ctccgcaaca tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct
      241 ggctctggga agtccactct tatccgtacg ctcaatggtt tagaggctgt tgacaaagga
      301 agtctcctag tcaatgggca ccaagttgct ggtgccagcc agaaagattt ggtacctctt
      361 cgcaaggaag tcggcatggt ttttcaacat tttaaccttt atccacacaa aacggtgtta
      421 gaaaacgtga cacttgcacc cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa
      481 accgcccaaa aatatctgga atttgtaaat atgtgggaca agaaagattc ctatcccgcc
      541 atactatctg gtggacaaaa acagcggatc gccatcgctc gtggtcttgc tatgcatccg
      601 gaactcctcc tctttgatga accaacatct gctcttgatc ctgagactat cggagatgtt
      661 ctagcagtta tgcagaaact ggcgcatgat gggatgaaca tgatcatcgt tacccacgaa
      721 atgggctttg ctcgagaggt tgcggaccgc attatcttta tggccgacgg agaagtttta
      781 gtagatacga cagatgtcga taactttttt gacaatccaa gcaaacctcg tgcccaacaa
      841 ttcctcagca aaattatcaa ccacgaaagt gacaaagtca aataaggagg cgcctatgaa
      901 aaaaaattct ttttatcagc attattgatt agccttttcg gccttgctgc tgccaaacca
      961 gtccaggctg atactaatat cgcagacatt caaaaaagag gcgaactggt tgtcggtgtc
     1021 aaacaagacg ttcccaattt tggttacaaa gatcccaaga ccggtactta ttctggtatc
     1081 gaaaccgact tggccaagat ggtagctgat gaactcaagg tcaagattcg ctatgtgccg
     1141 gttacagcac aaacccgcgg cccccttcta gacaatgaac aggtcgatat ggatatcgcg
     1201 acctttacca tcacggacga acgcaaaaaa ctctacaact ttaccagtcc ctactacaca
     1261 gacgcttctg gatttttggt caataaatct gccaaaatca aaaagattga ggacctaaac
     1321 ggcaaaacca tcggagtcgc ccaaggttct atcacccaac gcctgattac tgaactgggt
     1381 aaaaagaaag gtctgaagtt taaattcgtc gaacttggtt cctacccaga attgattact
     1441 tccctgcacg ctcatcgtat cgataccttt tccgttgacc gctctattct atctggctac
     1501 actagtaaac ggacagcact actagatgat agtttcaagc catctgacta cggtattgtt
     1561 accaagaaat caaatacaga gctcaacgac tatcttgata acttggttac taaatggagc
     1621 aaggatggta gtttgcagaa actttatgac cgttacaagc tcaaaccatc tagccatact
     1681 gcagattaag gaggacaccc catgacagat ttatcatctt ggacagccta ttttcaggat
     1741 tttggacaat ttttcaatgg tttcctcttc acccttgccc tagcggttgg ttcctttatc
     1801 ctcgctatgg ttttgggcat cttctttggt gccatgtcaa ccagtaaacg tccaatttta
     1861 cgcattttag ctcgcatctt tgttgaattt taccaaaata ctcccctctt ggtgcagttt
     1921 gttattgttt tttatggcct acctcttatc agtgaccata tcatcatgat tccaatttat
     1981 tggacagctg ttctctgcgt gggactctat cacggcgctt atatcgctga ggttattcgt
     2041 tcagggattc agtctattcc tagtggtcag atggaggccg ccttgtcgca aggttttacc
     2101 tatatcagtg ccatgcgctt gattatcttg cctcagacct tccgcatcat tctccctcca
     2161 ttgaccaacc aaattgttaa cctcatcaag aacacctcta ctgtagctat catctctgga
     2221 gtagacttga tgtttgtgac taagtcttgg tcggctctca acggaaacta tatcccagcc
     2281 tttttaggtg ctgctcttct ctactttgct ctatgcttcc ctgttgccca gtttggtcgc
     2341 aagatggagc aagccaacaa aaaagcctat tcactttagg aggttactat ggaatctatt
     2401 ttagaagttt taaccccaga taacctagtc tttatcttta aaggatttgg cttgaccctc
     2461 tatatttctc tgattgccat catcctctct actatcatcg gtacggtgct agctgtcatg
     2521 agaaatggca aaaatcctat cttacgcatt atttccagta tttatatcga gtttgtgcgc
     2581 aacgttccca accttctctg gatttttact atctttttgg tgttcaaaat gaagtccaca
     2641 ccagcaggta ttacagcctt tactctcttt acatcagcag ccttggctga gattattcga
     2701 ggcggtctca atgccgtaga caagggacaa tacgaagcag gaatgtcaca aggcttcacc
     2761 tcagcccaaa tcctctacta catcattctc ccacaagcca tccgcaaaat gctaccagcc
     2821 atcatttctc agtttgttcc cgtgattaag gataccagtc tcctctactc tgttatcgcc
     2881 ctacaagaac tctttggagc cagccaaatt ctcatgggcc gttatttcga accagagcag
     2941 gtcttcagtc tttacatcct gattgccctc atctacttca gctttaacct agcaatttct
     3001 agcctgtctc atatgctagc caaacgttgg caacaagctg cagaataata ctcttcgaaa
     3061 atctcttcaa accacgtcag cttcaccttg ccgtactcaa gtacagcctg cggctagctt
     3121 cctagtttgc tctttgattt tcattgagta taaacagcca ctctccagtt caattggaga
     3181 gttttttgat gagagtagat atttatagac ctcaatttga caccatacat tttctatgat
     3241 aaaatgtaac aaatttattt acaacaaatc gaaatctaaa tgaaatggaa ttttctatga
     3301 aatcagtcaa aggactactc tttatcatag ctagttttat cttgactctt ttgacttgga
     3361 tgaacacttc tccccaattc atgattccag gactagcttt aacaagccta tctctgactt
     3421 ttatcctagc cactcgtctc ccactactag aaagctggtt tcacggtttg gagaaggtct
     3481 acaccgtcca caaattcaca gcctttctct ccatcatcct actaatcttt cataacttta
     3541 gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt tggtaatctt gccatctata
     3601 tctttgccag catcatcctt gtcgcctatt taggcaaata catccaatac gaagcttggc
     3661 gatggattca ccgcctggtt tacctagcct atattttagg actctttcac atctacatga
     3721 taatgggcaa tcgtctcctt acatttaatc ttctaagttt tcttgttggt agctatgccc
     3781 ttttaggctt actagctggt ttttatatca tttttctata tcaaaagatt tccttcccct
     3841 atctaggaaa aattacccat ctcaaacgct taaatcacga tactagagaa attcaaatcc
     3901 atcttagcag acctttcaac tatcaatcag gacaatttgc ctttctaaag attttccaag
     3961 aaggctttga aagtgctccg catccctttt ctatctcagg aggtcatggt caaactcttt
     4021 actttactgt taaaaattca ggcgaccata ccaataatat ctatgataat cttcaagccg
     4081 gcagcaaagt aaccctagac agagcttacg gacacatgat catagaagaa ggacgagaaa
     4141 atcaggtttg gattgctgga ggtattggga tcaccccctt catctcttac atccgtgaac
     4201 atcctatttt agataaacag gttcacttct actatagctt ccgtggagat gaaaatgcag
     4261 tctacctaga tttactccgt aactatgctc agaaaaatcc taattttgaa ctccatctaa
     4321 tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa agaagtgccc gaacatgcaa
     4381 ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag attaagaaac
     4441 aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta atcatactct
     4501 cctggaattt cccaggagag ttttttatag ccaaatgaaa taagaccgga acgatggaat
     4561 tttggcattc aaatcaattg ataagaatat t
//
LOCUS       DAVKZB010000114         4543 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKZB010000114 DAVKZB010000000
VERSION     DAVKZB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.114"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(112..1098)
                     /locus_tag="VXA80_001749"
     CDS             complement(112..1098)
                     /locus_tag="VXA80_001749"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV0354200.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1292..3760
                     /gene="gyrA"
                     /locus_tag="VXA80_001750"
     CDS             1292..3760
                     /gene="gyrA"
                     /locus_tag="VXA80_001750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV0354201.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3760..4506
                     /gene="srtA"
                     /locus_tag="VXA80_001751"
     CDS             3760..4506
                     /gene="srtA"
                     /locus_tag="VXA80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV0354202.1"
                     /translation="MSQKKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIV
                     WNTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIP
                     ELEMNLPIFKGLDNVNLFYGAGTMKREQVMGKGNYSLASHHIFGVDNANKMLFSPLDN
                     AKNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERII
                     VKGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         1387 a          807 c         1018 g         1331 t
ORIGIN      
        1 acaacgtttt agagtaatgg ggtgctattt caacttcaac ctactataat acagaaaaaa
       61 atacagaaaa aaacaactcc ctgatgattc aaggagttgt ctatagttaa attagttttt
      121 agaagcttct tggaattctg gatttttcca tgcttcgtca ataatagctt gcaattcttt
      181 agcagatgct tgcatttttt gagtttctgc atcgttcaat ggaatgttta ctggacgaac
      241 gataccatgt gcaccaacaa cagctggttg accgataaag acattctcaa ctccgtattg
      301 accttcttgg aatactgaaa gtggaagtac tgcgttttca tcgtcaagga ttgctttagt
      361 gatacgagca agggctactg cgataccgta gtatgttgca ccttttttgt tgatgattgt
      421 gtaggctgca tcacgaacac cttcgaacaa ttcaatcaat tcagcttctt gaacattttg
      481 agtgtcttta aggaattctt caaggtttac accagcgatg ttagcgtgtg accaaacagc
      541 gaactcagag tcaccgtgtt cacccatgat gtaggcgtgc actgaacgag catccacatc
      601 caatttttca gcaagtgctt gacggaaacg agctgagtca agtgaagtac ctgaaccgat
      661 aacgcgttct ttagggaaac cagagaattt ccaagttgag taagtcaaaa cgtcaactgg
      721 gttagcagca acaaggaaga tacctttgaa accagattca acaacttgag ttacgattga
      781 tttgttgata gcaaggtttt tacctacaag gtcaagacga gtttcacctg gtttttgagg
      841 tgcacctgca gtgatcacaa caaggtcagc gtctgcacag tcagagtatt gagctgcata
      901 gattttttta ggtgaagtga aggcaagggc gtgactaagg tcaagcgcat caccaacagc
      961 tttttcatgc aattgtggaa tttcgataat tccaagctct tgtgcaattc cttggttaac
     1021 aagtgcaaaa gcgtaagatg aacctacagc accatcaccg acaaggataa cttttttgtg
     1081 ttgtttagtt gaagtcattg ttttaaacat ctccttaatt ttattagggg attttcccta
     1141 gacaacttca ttctatcact tttaaaaaac tttgtcacga atatgcctta tagttctcga
     1201 tgtaaacgtt ttagtggttt agaggctgaa atagatggga atttatggta taatgttgtt
     1261 acttactaat tgtgaaatga ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa
     1321 tctgacaaag gagatgaagg caagttttat cgactacgcc atgagtgtta tcgtagcgcg
     1381 agctcttcct gatgttcgag atggcttaaa acctgttcac cgtcgcattc tctacggaat
     1441 gaatgaattg ggtgtgaccc cagacaaacc ccataaaaaa tctgctcgta ttacagggga
     1501 tgtcatgggt aaatatcacc cacacgggga ttcctctatt tatgaagcca tggtccgtat
     1561 ggctcaatgg tggagctacc gttacatgct tgtagatggt catgggaatt ttggttccat
     1621 ggatggagat agtgctgccg ctcaacgtta taccgaggca cgtatgagca agattgctct
     1681 ggaaatgctt cgtgatatca acaaaaatac agttgatttc gttgataact atgatgccaa
     1741 tgaacgggaa cccttggtct tgccagcgcg ttttccaaac cttttggtta atggagcaac
     1801 tggtatcgcg gttgggatgg caaccaatat tccacctcat aatctgggtg aaaccattga
     1861 tgcagtgaag ttggtcatgg ataatcctga agtgactacc aaggacttga tggaagtctt
     1921 gcctggacca gattttccaa ctggtgctct tgtcatgggg aaatcaggta tccataaggc
     1981 ttatgaaaca ggtaaaggtt cgattgtcct acgttctcgt acagagattg aaacgactaa
     2041 gactggtcgt gagcgcatcg ttgtaacaga atttccttac atggtcaata aaaccaaggt
     2101 gcatgagcat attgttcgct tggttcagga aaaacgcatt gagggtatca cagcagtacg
     2161 tgatgagtca aaccgtgaag gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc
     2221 caatgttatt ctcaataacc tcttcaaaat gacccaaatg caaaccaatt ttggtttcaa
     2281 tatgctcgct atccaaaatg gtataccgaa aattttgtct cttcgtcaga ttttggatgc
     2341 ttatatcgag caccaaaaag aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc
     2401 ggaagcgcgc gctcatatct tagaaggtct cttgattgcg ctagaccata tcgacgaagt
     2461 gattcgtatc atccgtgcta gtgaaacgga tgcggaagct caagctgagt tgatgagcaa
     2521 gtttaagctt tctgaacgtc aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac
     2581 aggtttggaa cgcgataaga ttcaatctga gtatgatgac ctcttggctc tgattgcgga
     2641 tttagcagat attcttgcta agcctgaacg tgtttctcaa attatcaaag acgaattgga
     2701 tgaagttaaa cgtaaatttt ctgataaacg ccgtacagag ttgatggttg gacaggtctt
     2761 gagtctcgag gatgaggact tgattgaaga atcggatgtc ttgattaccc tttctaacag
     2821 aggctacatt aagcgtttgg atcaggacga gttcactgct caaaaacgtg ggggtcgtgg
     2881 tgtccaagga acgggagtga aagatgatga ctttgttcgt gagttagtgt caactagcac
     2941 ccatgatcat ctgctcttct tcacaaacaa gggacgtgtc tatcgtctta aaggttatga
     3001 aattcctgag tatggtcgga ctgccaaagg gctaccagta gtcaatctct tgaaattgga
     3061 tgaagacgaa agtattcaga cggttatcaa tgttgagtct gatcgcagtg atgatgctta
     3121 tctcttcttt acaacccgtc acggtattgt gaagagaacc agtgttaagg agtttgccaa
     3181 tattcgtcaa aatggtctca aagcgctgaa tttaaaggat gaagatgagt taatcaatgt
     3241 cttgttggca gaaggagata tggatattat cattggtacc aagtttggtt atgcagttcg
     3301 ctttaatcaa tcagccgttc gtggtatgag ccgtatcgcc actggtgtga aaggtgttaa
     3361 ccttcgtgaa ggagacacag ttgttggtgc cagcttgatt actgatcaag atgaggttct
     3421 tgttatcaca gaaaaaggat atggtaagcg tacagtcgct actgaatacc caacaaaagg
     3481 tcgtggtggt aagggaatgc agacagctaa aattaccgaa aaaaatggct tgctggccgg
     3541 tcttatgact gttcaagggg atgaggattt gatgattatc actgatacag gtgtcatgat
     3601 tcgaaccaat cttgccaata tttcacaaac aggacgtgca actatgggag ttaaagtaat
     3661 gcgcctggat caagatgctc agatagtgac tttcacaacg gttgcggtgg cagaaaaaga
     3721 agaagttggg acagaaaacg aaacagaagg tgaagcataa tgtctcaaaa aaaaaataat
     3781 aaaaagaaaa acaagcgaaa aaatttgctg acaaatatcc tagcaggatt tctgatatta
     3841 ctgtcactgg ctttgatttt taatactcaa attcgaaata ttttcatagt ctggaatacc
     3901 aataagtatc aagttagcca ggtatcaaaa gaaaaattag aagaaaatca ggatacagaa
     3961 ggcaattttg actttgattc tgtcaaagct atctcttcgg aagctgttct aacttctcaa
     4021 tgggatgctc aaaaattacc agttattggg ggaattgcaa ttcctgaatt ggaaatgaat
     4081 ttgccgattt ttaaaggact tgataatgtt aatctcttct acggagctgg tacaatgaaa
     4141 cgcgagcaag taatgggaaa aggaaattat agtctagcta gtcaccatat ctttggtgtt
     4201 gataatgcta ataaaatgtt attttctcct ttagataatg ctaaaaatgg catgaagatt
     4261 tatctaaccg ataaaaataa agtttatact tatgaaatac gtgaagtcaa acgtgtgaca
     4321 ccggatcgtg ttgatgaagt tgatgataga gatggggtca atgaaatcac attagtaacc
     4381 tgtgaagacc ttgctgctac agaacgtatt attgtcaaag gtgatttgaa agaaacaaaa
     4441 gattattcac aaacatctaa tgaaatccta acagctttca atcaaccata taaacaattt
     4501 tattaataca aatcagtgaa atcatggatt tcactgattt ttt
//
LOCUS       DAVKZB010000115         4520 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKZB010000115 DAVKZB010000000
VERSION     DAVKZB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.115"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            16..1225
                     /gene="sstT"
                     /locus_tag="VXA80_001752"
                     /pseudo
     CDS             16..1225
                     /gene="sstT"
                     /locus_tag="VXA80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1293..2246
                     /locus_tag="VXA80_001753"
     CDS             1293..2246
                     /locus_tag="VXA80_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0354203.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2389..2769
                     /locus_tag="VXA80_001754"
     CDS             2389..2769
                     /locus_tag="VXA80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0354204.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            2868..3089
                     /locus_tag="VXA80_001755"
     CDS             2868..3089
                     /locus_tag="VXA80_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0354205.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3106..3420
                     /gene="trxA"
                     /locus_tag="VXA80_001756"
     CDS             3106..3420
                     /gene="trxA"
                     /locus_tag="VXA80_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0354206.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3727..4260
                     /gene="queF"
                     /locus_tag="VXA80_001757"
     CDS             3727..4260
                     /gene="queF"
                     /locus_tag="VXA80_001757"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV0354207.1"
                     /translation="MKRQLALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            4322..>4520
                     /locus_tag="VXA80_001758"
     CDS             4322..>4520
                     /locus_tag="VXA80_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354208.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDKVSLKHCFEVADRTDEVSLK
                     HCFEVADRTDEVSLKHCFEVVD"
BASE COUNT         1290 a         1007 c          891 g         1332 t
ORIGIN      
        1 aaaaaggaga ttattatgaa aagaatcatt catgcttgga ataaggcaag ccttgtcaag
       61 cgtatcttga ttggtatgct ttgtggggaa tcctaggact gacccttcct aatctctcag
      121 ggattgggct actcggggat ttattcgttg gaggtctgaa agctgttgct cctatcctag
      181 tctttgccct cgttgccaat gccctttccc aacatcaaaa gggacaagat agcaatatga
      241 aaactgttgt tttcttgtat atcctatgat agggactttc gccgctgctc ttgtagctgt
      301 actagcaagt ttcatcgtcc ctattgaaat taccctaaat agtgccaata ctgaaattgc
      361 accaccagat gggattgggc aggtcctcag caacctcttg ctcaaactgg ttgacagccc
      421 agtcaacgcc ctgcttactg ctaactatat tggaatctta tcttgggcag tcatttttgg
      481 aatcgctatg agagaagcta gtaaaaatag taaagaattg ctaaaaacta tcgctgacgt
      541 gacttctaaa attgtcgaat ggatcatcaa tctggctcca tttggaatcc ttggtcttgt
      601 ttttaaagcc atttctgaca agggagtcgg aagccttgcc aactacggta ttttattggt
      661 tccattagta acgactatgc tttttgttgc ccctgtggtc aaccctttga ttgccttctt
      721 ctttatgaga cgcaatcctt actctctagt ttggaactgc ctccgtgtca gcggtgtgac
      781 agcctttttc actcgtagtt ctgcgactaa cattcctgtc aacatgaaac tctgccatga
      841 ccttggactc aacccagata cctattctgt ttctatccca ctcggttcta ctatcaatat
      901 ggctggagta gcgattacca ttaacctttt gacccttgct gcagttaaca ctcttggaat
      961 tcctgttgac tttgccacag cctttgtcct cagtgtggta gcagctatct catcctgtga
     1021 tgcttcaggt attgccggag gttccctcct tcttatccca gttgcttgta gccttttcgg
     1081 tatttctaac gatattgcca tacaaattgt tggggttggt tttgtgattg gtgtcatcca
     1141 agactcatgt gaaacagccc ttaactcttc tacagatgtc ctctttaccg ccgttgccga
     1201 atacgcagca acccgtaaaa aataactcat caaggcaagc ctgctcatgt cttgtctttt
     1261 acgcttttat tctaacttat taggaaattc ttatgtctat tagccaacgt acgaccaagc
     1321 tcatcttagc tacctgtctt gcctgcctgc ttgcttattt tctcaatctt tcgtcagcag
     1381 tttcggctgg aattatcgct ctcttgagcc tatctgatac gcgtagaagt actttaaaac
     1441 tggctcgcaa tcgtcttttt tctatgcttc tagctctggc tatcggtgtt ctagcttttc
     1501 acttgagcgg atttcatatc tggagtctcg gcctctatct ggccttctac gttcctttag
     1561 cctacaagat gggctgggaa attggcatca caccaagcac tgttttggtt agccatctct
     1621 tggttcaaga gtcaacctct ccagaccttc tagtcaatga attccttctc tttgctattg
     1681 gtacaggatt tgccttgctt gttaatctct atatgccttc acgagaagag gaaatccagc
     1741 actaccacac gctggtggaa gaaaagttaa aagatatcct ccagcgcttc aaatactatt
     1801 tatccagagg agacggacgc aaccgagcac agctggtagc agaattagac acgcttttga
     1861 aagaagccct cagactggtc tatttggatc actctgacca cctctttcac cagaccgact
     1921 accatatcca ctactttgag atgagacagc gacaaagtcg tatcctgaga aacatggccc
     1981 aacagattaa cacttgtcac cttgccgcca gtgaaagcct gatcttagcg caactctttt
     2041 caaaaattgc aggtcaactg agccagacca atcctgcttc tgatttgcta gatgaaattg
     2101 aacgttatct ggaagtcttc cggaaccgca gtctgcccaa gacaagagaa gaatttgaaa
     2161 cccgcgccac ccttcttcaa ctcctacgtg aagccaaaac cttcatccaa gtaaaagttg
     2221 atttttacca aaaatataga cagtaaaaaa taaaaccact aaaaaacaga gcttgccagc
     2281 attcttgaaa agtagtataa ttattgcatg cgcaatcatc ttgtgacgca gagactgccc
     2341 gttaatggac tttcttctat ttatgactct aaaaagaaag gagacactat gacctatttg
     2401 gaaaaatggt ttgacttcaa tcggcgtcag aaagaaattg aaagtctctt ggaagagacc
     2461 gttgcccaac agagcgagca aagtctgacc ttgaaagagt tctacctgct ctactatctg
     2521 gacttggctg aagaaaaatc tctacgccag attgacctgc cagataagct tcatctgagc
     2581 ccgagcgcag tttctaggat ggtggctcgc ttggaagcta aaaattgcgg tctactcagt
     2641 cgcatgtgtt gccatcaaga tagacgctct agttttatct gcctgacaaa tgatgggcaa
     2701 aagatactgg cctcgctaca aaagactgtc gaagaaagct tggaagcagg tttggatttc
     2761 ttgatttaag atgattttag aaaaagttgc gtgcgcaatc atttttcttg acattctctt
     2821 ttataaggag taaaataaag ttagcattaa acaaaggagt tttaaatatg attgaaatta
     2881 cctatataga tgccagcaag aacgaaagaa ctgtgacttt cgagtcttat gaagactttg
     2941 aacgttcgca acaagcttgc cttatcggcg tcgcagacta ctaccctgtc caaaaattaa
     3001 cttacaaggg tcataatttg gactaccatg ggacttatgg agatatcttc ttctatctca
     3061 tgaaacaaga tttaagccaa tataactaaa aaaaggagaa atacaatggc aaaagcaatt
     3121 acagatgcaa cattcgaaca agaaacaaaa gacggtttgg tcttagtaga cttctgggca
     3181 acttggtgtg gtccatgtcg tatgcaaggt ccaatcttgg acaaattgtc tgaagaactt
     3241 tcagaagatg tcttgaaaat cgttaaaatg gacgttgatg aaaatccaaa cacagctcgt
     3301 gcttttggaa tcatgtctat tccaactctt ctcttcaaaa aagacggcca agttgtcaaa
     3361 caagttgcag gtgttcacac agcagaacaa atcaaggcca tcattgctga attgagctaa
     3421 tcaaacgaga gaccaagtac tttctttggt ctcttttttc ttgccctttg ccatttttca
     3481 aaaaatatgc tagactatag cagattgaaa ctagaatagt acacctctac ttctaaaaca
     3541 ttgttagaaa tcgatttgac tgtcctgatc gatttatcct gttcttattt cattttacta
     3601 tataagtaga atattacgat gtttgggata tgccatcgct aaaaaaaaac tcgatttggt
     3661 attttttagc tccctaatgg gagctttttg catgctctga acatttccca ttttggaagg
     3721 agtactatga aacgtcaatt agccttggtc gtctttagtg gtggtcaaga ttcaacaacc
     3781 tgccttttct gggtcatgca acactatgaa acagtcgaag ctgtcacctt tgcctacggc
     3841 caacgtcatc acctcgaaat tcaaattact agagaaatcg ctaaggaaca gggcattcgt
     3901 caccatatcc tcgatatgtc tctgctggga caaatcactg ctcagccaga ctttgcgacg
     3961 attcatattt cctacattcc tgacaagctc tgtgtcgagt caaaatccct caaactatat
     4021 ctatttagct accgaaacca cggagatttc cacgaaaact gtatcaacac catcgggaaa
     4081 gacttggtca acttgctaga ccctcgctat ttagaagtct ggggaaaatt cactccgcgc
     4141 ggtggcattt caatcgaccc ctactataac tacggtaagc aaggaactaa gtatgagggc
     4201 ttggcagaac aacgcctctt ccaacacgac ctttatccag agaaaattga caaccgctaa
     4261 agtcatactc aatgaaaatc aaagagcaaa ctaggaagtt agccgcaggt tactcaaagc
     4321 agtgtttgag gttgtagata aaactgacga agtcagctca aaacactgtt ttgaggttgc
     4381 agatagaact gacaaagtca gcttaaaaca ctgttttgag gttgcagata gaactgacga
     4441 agtcagctta aaacactgtt ttgaggttgc agatagaact gacgaagtca gcttaaaaca
     4501 ctgttttgaa gttgtagatg
//
LOCUS       DAVKZB010000116         4483 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKZB010000116 DAVKZB010000000
VERSION     DAVKZB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.116"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(1..462)
                     /locus_tag="VXA80_001759"
                     /pseudo
     CDS             complement(1..462)
                     /locus_tag="VXA80_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(452..1237)
                     /locus_tag="VXA80_001760"
     CDS             complement(452..1237)
                     /locus_tag="VXA80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354209.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPFVSEFWNDFKNNH
                     DKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAI"
     gene            complement(1645..2070)
                     /locus_tag="VXA80_001761"
     CDS             complement(1645..2070)
                     /locus_tag="VXA80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354210.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2204..2557)
                     /locus_tag="VXA80_001762"
     CDS             complement(2204..2557)
                     /locus_tag="VXA80_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354211.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKNKCY"
     gene            complement(2603..2809)
                     /locus_tag="VXA80_001763"
     CDS             complement(2603..2809)
                     /locus_tag="VXA80_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354212.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(2796..2957)
                     /locus_tag="VXA80_001764"
     CDS             complement(2796..2957)
                     /locus_tag="VXA80_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354213.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3315..4271)
                     /gene="lytA"
                     /locus_tag="VXA80_001765"
     CDS             complement(3315..4271)
                     /gene="lytA"
                     /locus_tag="VXA80_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0354214.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
BASE COUNT         1363 a          954 c          633 g         1533 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc ttttacatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttgtcaa aatataaggg
      121 aatttctcga ccgtccttct ttaatggata ttctggtaag atactaaata gttcaatcat
      181 agaaggaaac tcagaattag ttgttgtaaa tcgaaaaagc tgcgctttct catctttctg
      241 acttccttga tactctccca attctaaaaa atgattcaag gtagtataaa attccttatt
      301 ttttacttca tcaatgatga ccatatcata atcttttgtt gtgcgacttt taaatccttg
      361 cgaatccaat acgatagagg tagcagttcc cccaatcaga acataatagt tctgaaaatc
      421 cgcaaacatt tcttgaaaaa taactttcgt attatatggc atcatcttct cccagatact
      481 gtaatatcat attttctagt gcttcactct cttcctctat acgtgggtca tcatcatctt
      541 ttaaggtcaa ataaagagaa atcggatcta caaattgttt atcatgatta tttttaaaat
      601 cattccaaaa ctcagataca aaaggacgat atttccatat ctctagcatc tttcctttta
      661 aaacatgctg agaaagtggc aaggataact gattgaattt tctctgccat atgacatagc
      721 tagtattttc atccgtttca gctaaaaaag ttgaatgcga caaagcataa gcaccaccat
      781 atagaaggtt agaaacagat tttatctgct ttatatcgcc atctggcaat aaaaaggcaa
      841 tccacgttaa ttgttcgcta ggtgttaatt ccttagggat ttcagtatca ttcgcattga
      901 gtactagtcc caatggaggg aagaagaggt ttcccttaaa gtctacaaac ggaactctag
      961 cttgaagtaa ttgctttttt tcactgtctg ataacttcga aaacaccaaa acaacatcca
     1021 tattggcttt ttcacccata gtgcgagcct gagtaacaaa tgaactcaaa ctcccccttc
     1081 tcttttcttt aatcaataaa aatgattcct tatgaaaagt catttgagta tactcaaacc
     1141 tttttacaaa actgattggt aaagtcaaag tcagttcctc aatactgaca tcaacataaa
     1201 aggattgaaa aattgacatt atctttttga cattcatgtt ttttccttat tctatcattt
     1261 ttattatatt ttatcattcg gtataataaa atataagttt tttgataaaa taaccaaaaa
     1321 ctactcgcta tcccctctca cattacccta catatcgttt gacatgcgac tgatagttca
     1381 ggaaaacttc aaggagaact tttctctcat ccactatgca ggacttacta tttcacttct
     1441 actcctatag gctcaatttg acacttttct ttttcgaatt gctcatatgc ctcctgaata
     1501 gtgagttcga tttatccaaa tttatttttg tctattttga tgaatacttc cacgaagata
     1561 gtctgaaaat gaatttgcac caattttcct tctcttttta agcatattat tccagatatg
     1621 attgccctct tgttcagtaa taaattacgc tttaaacgct tcaatcagta tatctcctgt
     1681 tgtcatatgt tctaaagaaa attcttctac atatggttta acatctctta ggttattact
     1741 tcctaatatc ccattatgct ttttcgctaa gaaaatagaa gtcgcttctc ccttaccaat
     1801 aatcttgtta ctatcatgat ttcttgttaa atctctatat aatgcgtatt cttcagttcc
     1861 aatgtctatg ctcacaatct cagctgaacc cttagctacc aactgatcta tcctagattt
     1921 taaatgggga attgtaggta tattgatttc atcatacacc tcttgtggaa taacaatttt
     1981 acccaaatag agcttttcta aaagatgttc agtgccaacc cataaaaata ctgaaataca
     2041 atctgcatca ataaatacac gactagtcaa acgacaactc ccccctcttc atctagccca
     2101 tatacaatat catatctgaa agcatctagt aacagttcct catacttccc ttgcgaaatt
     2161 ctgttatttt ctaaaagttg ttcagttgta ttaatatatt atcctaataa catttatttt
     2221 tactttctga caaaggacga tataaacttg tatcatagcc taatcttgaa gctgtctcta
     2281 taacactaat atccatattt ttaatttctt ctgcatcaag gtatccatca ttcctcaatc
     2341 tatataacat agctttatga ctgataccat aaaactgacc caattttata atatcttcta
     2401 cttcaagatg agttctattg gcattttctc tgatttcctc aaccatccta tacagtgaag
     2461 atgggaaaat taaaaaataa gaagcaaact gatccgcttt tctttcagtt tcatctcctt
     2521 caccaatcaa gataagactg actgaactct tcttcacctc atcataataa agatgataca
     2581 gtccatgtgc taaagaaaat ctttaccttc ctaatggcat gtctgaattg actgcaatga
     2641 gactgaactg agttccttta taacagactc cgctaatatt ctttccgagt ccataaaata
     2701 ccagcgtcaa attttctatc ttttgtacca atttaaaaat atctatcggc gattcaccat
     2761 cagctcccaa cttttttcta agatttgaag ctttattact taaatccatt cgtgatattc
     2821 ctttcacctt tttccatttt ggcaatcatg ctttgattca aagacaaata ctcagcaatc
     2881 ttatcttgag taagatttga agatattcta agttctttta ttctttttcc aacatcacat
     2941 acatttatca catacattta tcatattcat acacctctca taaaaagaat agcacactct
     3001 tgtcataatt ttttaaataa aaaaattatg acaaaacaag gaagcaattt attgatgctg
     3061 cttaaaaatc taaaattgat gagttcctat caaaaatcgt atcttcaacc tcaaaacagt
     3121 acttaaagct atccgactcg gtttacattg tcaaatttag attttatttg agcataactt
     3181 tctagtttgc tttttgattt ttgtttaata tagtagcaaa attgaaggaa atatctccac
     3241 aagaaaacgc atactattaa gctttttcaa gacctaataa tatgcgctgt tctgatttga
     3301 aagacattcc attattattt tactgtaatc aagccatctg gctctactgt gaattctggc
     3361 ttgtctgcca gtgttccgtc tggtttgagg tagtaccagc ctgttccgtc cgctgactgg
     3421 ataaaggcat ttgataccat ggcgccttct ttagcgtcta agtagtacca agtgtccttg
     3481 tacttgaccc agcctgtctt catggcacct tcttcgttga aatagtacca cttatcagcg
     3541 attttcttcc agcctgtagc catttcgcct gagttgtcga accagtacca gttgccgtct
     3601 gtgtgcttcc tccagcggtc tgcaagcata tagcctgaac tgtcaaagta gtaccaagtg
     3661 ccattgattt tctcaaactt gtcttttgga taagagccgt ctgaatgtac gtaccagtag
     3721 ccagtgtcat tcttctgcca gcctgtttca atcgtcaagc cgttctcaat atcatgctta
     3781 aactgctcac ggctaatgcc ccatttagca agatatggat aagggtcaac gtggtctgag
     3841 tggttgtttg gttggttatt cgtgcaatac tcgtgcgttt taattccagc taaactccct
     3901 gtatcaagcg ttttcggcaa acctgcttca tctgctagat tgcgtaagag ttcgatataa
     3961 aggcggtagt ccgtcatgaa ctcttctttg gttgaatggc tttcaatcag ttcaaccgct
     4021 gcataggtct cagcattcca accgccccca acgtcccagg caccattatc aacaggtcct
     4081 acctgcatga tgcaaccgtt cccaacaatg tgcgagaaaa aacctaattc tgggtctttc
     4141 cgccagtgat aatccgcttc attctgtacg gttgaatgcg gattcccagt tgagtgtgcg
     4201 tgtacttgcc tatatggttg cacgccgact tgaggcaaat ctgttcttaa tttactcaca
     4261 ttaatttcca tattctactc cttatcaatt aaaacaactc attttttaca atccagaacc
     4321 agaaactcct ttatttctac cttacaaaga agacaatctt agtcacgatt aggcttgtag
     4381 atagaacttc aaaacgcact attttgacac tgtaaatagg actgacaagg tctgcattct
     4441 atctacaata acaccccaga ctaaaaagat tttcaaaagt ata
//
LOCUS       DAVKZB010000117         4441 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKZB010000117 DAVKZB010000000
VERSION     DAVKZB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.117"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            132..1367
                     /locus_tag="VXA80_001766"
     CDS             132..1367
                     /locus_tag="VXA80_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354215.1"
                     /translation="MKLYRCWYQTFVSDEASEETPLAERGEKEYIVKSSRMTALCYAE
                     SKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRYKLVSKLEFSP
                     NLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVITRTWANNVRLS
                     AITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHEGDTLNENYDK
                     VDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPIIEDDKIWFYY
                     MGGNGNHTDFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYDDEIYILADIP
                     ENGYVKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLGNSPVAFKFEI
                     ENAKLYGIEGSIQQHYLKY"
     gene            1436..3013
                     /locus_tag="VXA80_001767"
     CDS             1436..3013
                     /locus_tag="VXA80_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0354216.1"
                     /translation="MVKLNKVLKGSVALLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDQTLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSKHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            3076..>4441
                     /locus_tag="VXA80_001768"
     CDS             3076..>4441
                     /locus_tag="VXA80_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV0354217.1"
                     /translation="MYDVVVIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIEKGGTVFSSMEELMNVSDFYQITIPYQPE
                     VYESVLTEMLNESNVDILLNVEINNIVFDNNKIHSVEIISNQEVNYLESKVFIDATGN
                     ATIASASGANVYEKNSSYGCLMRVGGVDITKTLEHIEKTKQWEFEEGYNEWLDNVLAN
                     RKYRATGAVLRDPVSYDHAPMLTKDDHFMNEEKWSYIKERWERFGFIYTLEVSLFRNE
                     LREAVEAGDFEFEIYHDDESGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIE"
BASE COUNT         1581 a          611 c          866 g         1383 t
ORIGIN      
        1 taaaattaaa taagctaaaa aaagatactg aaaacaatcc actatttggt gaagagtatt
       61 ttgccgaaaa tcctaaatta tgggaagttc gttttgataa ttcatatcca aacgttatct
      121 atgataaaga attgaaatta tatagatgtt ggtatcaaac atttgttagt gatgaagcaa
      181 gcgaagagac tcctttagca gaacgtggag agaaggaata cattgtcaag tcatctagaa
      241 tgacagcact ttgctatgct gaaagtaaag atggagtaaa atgggaaaaa ccaaatctca
      301 atctagtaaa atttaaaggt tcaaaagata ataatattgt tctgatgcat gcacacggtg
      361 caggtatcat gctagatgaa caggagttgg atcctaagaa acgatataaa ttagtttcaa
      421 aattagaatt ttctccgaat cttcattaca tggttgttgt tttttcagaa gacggtattc
      481 attttgacga acctattcca tggaaaaaat ggaatcctgc agcagatagt cataattttc
      541 catttagaga tccaaaaact ggtaaatacg ctgttattac aagaacctgg gcaaataacg
      601 tacgtctaag tgcaattact ttcagtgatg attttattaa ttggtcagaa cctaaagaaa
      661 tccttagggg agatggattt gaagatcaaa tttattctat gccagtaatt tcatacgcgg
      721 atatctattt aggttttgct tcaatgtacc acgaaggcga cactttaaat gaaaactatg
      781 ataaagtaga cttagaattg aaattctctg ctaatttgga acaatgggat agtgtatgta
      841 agggcgacta tctcattgaa aggggaggtg gagtataccc gcagaatgct gattttgatg
      901 ctagttgtat tttcacggct gctccaataa ttgaagatga taagatttgg ttctactata
      961 tgggtggaaa tggtaatcac actgacttcc gagaaagttc attagctaga ggatatatta
     1021 agaaagacca atttgcttat tatactaata aaagagaagg aattccagct aaaatatcta
     1081 cagttagctt ccaaacttac gatgatgaaa tttatatctt agctgatatt cctgaaaatg
     1141 gttatgttaa agttgcggta ggtactaaat acggtaatgc ttattcagat tttggtcagg
     1201 aagatatctc tcttgttcaa gtttccgatg gaaagtataa gttggaattt aataaaaaat
     1261 ctatcttaga tttaggtaat tctccagttg cgttcaaatt tgaaatcgag aatgcaaaat
     1321 tgtatggtat tgaaggtagt attcagcaac actatctcaa atactaacca gattaattcg
     1381 ttaacaaata atctctatat tagtggagat agatatataa aatggaggtc ttattatggt
     1441 aaagcttaat aaggtattaa aagggagtgt agcactcctg agtgcaagtt tattgctagc
     1501 agcttgtggc aataacgcta attcaaagcc tgctgaaaaa acagctgatg gtaaaacgaa
     1561 aatttcgttt ctatctacat ggcaaattaa tgatgttcgt ccacctaaag atcaaacact
     1621 tgttggtcag tggttagaag aacagctagg tattgatatt gatcttgtaa ctattcctgg
     1681 cgagggagta agttctaaag taaattctct tattactagt aaccagttac cgactgtttt
     1741 gttgacaaca ggagataaga gtgatatcgc tggtatcaac aaacttggta aacagggtgc
     1801 attcttagat ttgtcaaaac acatggacaa actaccaaac tataagaagt accttgaaaa
     1861 aaataatgca ttagctcaaa tagaagatga tgaaaaacat atttatgctt tcacaaaatt
     1921 ctttactgat gagaatatca tgtatactac accaattctg agaaaagact tacttgttgg
     1981 ttcagaattt gaagatcttt caaaaattaa aactgttgat gactatacaa aagttttgaa
     2041 atatttgact gaaaaacaag gttctccagc atttattcaa cgtaatggct atgaaggctt
     2101 catgaagcga gttacaccat tgtggaattt aagccatcgt acatactatg actatgaatc
     2161 agattcatat aaacatccag ttgaacaacc tcaattgaaa caattcgttg aatggttgaa
     2221 agaattacgt aaagataacg tacttcaccc tgactgggca gttatgaaag atgaaacttg
     2281 ggaaggtcta ttggcttcta acaagggttc attcactgtt gaccgtatga atatcattgg
     2341 agataataac tttgataaat catttgattg gcagccactt gtttatccag aaattaatgg
     2401 aaaacgttat ttgcaaccga aaacaccgta catttctgat tcaggttggg taatcaatgc
     2461 caatgcacca aaagaagaaa ttgataaagc gttagaattc tttaatttct tatacgctga
     2521 tgaaaatcaa gaaaaacttg ctattggatt tgaaggtaaa acatttacac gtgaaaaccc
     2581 taatactcaa gcaggtatta aatggttgat tcaaatttac ggtgaaaatg ctggaaatga
     2641 taaagcagaa ctgctgttta aacatggtaa ccaagcattt actcgcgttc aaactaaatt
     2701 agatattgag gcacagccag gaacattccc taaagttatg tatgatcaag tagataaaat
     2761 taagaaagat ttaggtggtt ttagaccaga tactccggtt ctttccttta caactgaaga
     2821 attagatgtt ttaactaaat ctgaagcagg attaaatact ttctttgatg aaaatattat
     2881 taaattcatt gaaggacaaa gagatatgag tgagtgggat tcatttatca atgaagctaa
     2941 atctaaagac ttggataaag ttacaaaagt atataatgat gcctacaaac gtgtcaaaga
     3001 tagaatgaaa tagattaaat ggtagctctc tgattattca gggagctact aagcctagaa
     3061 agtgaatatt cgaaaatgta tgatgttgta gttataggtg ctggtagttc aggtgctctt
     3121 gcagcaattg cttctggtcg cctaggatta aaaacctgtt taatcgaaaa agggagtcgt
     3181 ataggtggcg taccgattaa tactctaatg ggttcatttg caaatcttta cttcgatgat
     3241 tctgggagag taaatgccag taagattgtt ggtgagttaa ttgaacgtat tatagaaaaa
     3301 ggtggaactg ttttttcttc gatggaagaa ttgatgaatg taagcgactt ttatcaaatc
     3361 acaatcccgt accaacctga agtctatgaa tcagttctaa cagaaatgtt aaatgaatct
     3421 aatgttgata ttcttctgaa tgtagaaatt aacaatatag tttttgacaa caataagata
     3481 catagtgtag aaatcatcag taatcaagaa gtaaattatc ttgaatcaaa agtttttatt
     3541 gatgctacag gtaatgctac gatagcttca gcaagtggcg ctaatgtata tgaaaaaaat
     3601 tcttcttatg gctgtttaat gagagtgggt ggagttgaca tcactaagac tcttgaacat
     3661 attgaaaaaa cgaagcagtg ggaattcgaa gaaggttata atgaatggct agataatgta
     3721 ttggctaacc gaaagtatag agctacaggc gcagtattga gagatcccgt ttcttatgat
     3781 catgctccta tgttaactaa agatgaccat tttatgaatg aagaaaaatg gagttatatc
     3841 aaggaaaggt gggagagatt cgggtttata tatactttag aagtatcatt gtttagaaat
     3901 gaattaagag aagcagttga agctggtgat tttgagtttg aaatttatca tgatgatgaa
     3961 tctggagtaa ctatgaacgg tgatggtatc agttatggtt catggggtca aaatgtagca
     4021 ttaattaatg ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat
     4081 gctaatattc tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta
     4141 ccaggttttg aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt
     4201 aggcacattg ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt
     4261 aagactatgt atagctatca aactcctaaa gaaataacgt ataaatcaat tgtaggtgga
     4321 aaattgaata atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca
     4381 caaattagtt gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa
     4441 g
//
LOCUS       DAVKZB010000118         4397 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKZB010000118 DAVKZB010000000
VERSION     DAVKZB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.118"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            433..1237
                     /locus_tag="VXA80_001769"
                     /pseudo
     CDS             433..1237
                     /locus_tag="VXA80_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1284..3938
                     /gene="pspC"
                     /locus_tag="VXA80_001770"
     CDS             1284..3938
                     /gene="pspC"
                     /locus_tag="VXA80_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV0354218.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDLSKKYADEVESHLKKILSEIQTQLDRKRHTKTVALINELQDIKKTYLY
                     NLNVLKEKSKAELPSKIKAKLDVAFEQFKKDTLKPGEKVAEAEKKVEEAKKKAKAQKE
                     EDFRNYPTNTYKTLELEIAESDVKVKEAELELVNEEVKEPLNEGTIKQAKEKVESEKA
                     EAIRLEEIKTDRKKAEATRLEKIKTDRKKAEEEEAKRQADAKEQEKPKRRAKRGVPGV
                     LATPDKKENDAKSSDSSVGEETLPSPSLNMANKSQTEHRKAAKQVDEYIEKMLSEIQL
                     DKRKHTQNFALNIKLSRIKTEYLYGLKEKSEAELPSKIKEKLDAAFEQFKKEPELTKK
                     VAEAKQKAEEAEKKAKAQKEEDFRNYPTNTYKTLELEIAESDVKVKEAELELVKEEAK
                     EHRNEEKIKQVKAKVESKKAEATRLEKIKTDRKKAAEAKRKAAEAEKKAAEAKPKVDA
                     EEYALEAKIAELEYEVQRLEKELKEIDESDSEDYVKEGLCAPLQSKLDTKKAKLSKLE
                     ELSDKIDELDAEIAKLEDQLKDAEKNNNVEDYVKEGLEKTIAEKKAELEKTEADLKKV
                     ANEPETPAPAPAPEKPETPAEKPALAPAPKPEKPTEQPKAEKPDDQQAEEDYARRSEE
                     EYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNA
                     NGAMAIGWLQNNGSWYYLNANGAMATGWLEYNGSWYYLNANGAMATGWLEYNGSWYYL
                     NSNGAMATGWLQYNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKTSQWFKVSDKW
                     YYVNGSGALAVNTTVDDYRVNANGEWVN"
BASE COUNT         1657 a          747 c          948 g         1045 t
ORIGIN      
        1 tagtacaccc ccacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg
       61 tcctgttctt gtttcatttt actatagaat gatttgaagg cgtttataat atttagctgt
      121 acgagagtct tttaaaagtg ttttgatggt ttggatttct tctttagttg atttcatatc
      181 actattatat aatgcttttt gattttagtc tagtataaat agcgagaaat atctatccta
      241 tcttctagaa tgtcttccaa acgaggaaac tctcgtaaac aaagaggttt tagaggccta
      301 tttaccatgg actaaagttg tacaagaaaa gtgcaaataa gaaatctcca gattaggaac
      361 tatccgtgag ttctctagtc tggagatttt tcaatagact tcctgcgaaa caaaatatgg
      421 tataatagtt ctatgaatta tgaagcaagt aaacaactaa ccgatgcacg atttaagcgt
      481 cttgttggtg ttcaacgcac gacttttgaa gagatgttag ctgtattaaa aacagcttat
      541 caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ctttcttatg
      601 gccactcttc aatatgtgcg agaataccgc acttatgaac aaattgcggc tgattttggt
      661 attcacgaaa gcaacttaat ccgtcggagc taatgggttg aagtaactct tgttcaaagt
      721 ggttttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg
      781 gaagtaaaaa tcaatcgccc taaaaaaaca attagcgaat tattctggta aaaagaaatg
      841 ccacgctatg aaggctcaag cgattgtcac aagtcaaggg agaattgttt ctttggatat
      901 cgctgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atatcggaca
      961 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
     1021 acaaactcca cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aagcttataa
     1081 ccatgcgcta tccaaggaga aaagcaaggt tgagaacatc tttgccaaag tagaaacgtt
     1141 taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac gaatgaattt
     1201 gattgctggc attatcaatc atgaactagg attctagttt tgcagggagt ctaatgttaa
     1261 gaaaaatatt ggaggaaata actatgtttg catcaaaaag cgaaagaaaa gtacattatt
     1321 caattcgtaa atttagtatt ggagtagcta gtgtagtagt tgctagcttg ttcttaggag
     1381 gagtagttca tgcagaagag gttggtggta ggaatacccc cacggttaca tctagtgggc
     1441 aagatttatc gaagaagtat gctgatgaag tcgagtcgca tctaaaaaaa atattgagtg
     1501 agatccaaac acaattagat agaaaacgac ataccaaaac tgtagcgcta attaacgagt
     1561 tgcaagacat taagaaaacg tatttgtata atttaaatgt tttaaaagag aagtcgaaag
     1621 ctgagttgcc gtcaaaaata aaagcaaagt tagacgtagc ttttgagcag tttaaaaaag
     1681 atacattgaa accaggagaa aaggtagcag aagctgagaa gaaggttgaa gaagctaaga
     1741 aaaaagccaa ggctcaaaaa gaagaagatt tccgtaacta cccaaccaat acttacaaaa
     1801 cgcttgaact tgaaattgct gagtccgatg tgaaagttaa agaagcggag cttgaactag
     1861 taaacgagga agttaaggaa cctttaaacg agggcacaat taagcaagca aaagagaaag
     1921 ttgagagtga aaaagctgag gctataaggt tagaagaaat caagacagat cgtaaaaaag
     1981 cagaggctac aaggttagaa aaaatcaaga cagatcgtaa aaaagcagaa gaagaagaag
     2041 ctaaacgaca agcagatgct aaagagcaag aaaaaccaaa gaggcgggca aaacgaggag
     2101 ttcctggagt gctagcaaca cctgataaaa aagaaaatga tgcgaagtct tcagattcta
     2161 gcgtaggtga agaaactctt ccaagcccat cccttaatat ggcaaataaa agtcagacag
     2221 aacataggaa agctgctaaa caagtcgatg aatatataga aaaaatgttg agtgagattc
     2281 aattagataa aagaaaacat acccaaaatt tcgccttaaa tataaagttg agcagaatta
     2341 aaacggagta tttgtatgga ttaaaagaga agtcggaagc tgagttgccg tcaaaaataa
     2401 aagaaaagtt agacgcagct tttgagcagt ttaaaaaaga accagaactt actaaaaaag
     2461 tagcagaagc taaacaaaaa gcagaagaag ctgagaaaaa agccaaggct caaaaagaag
     2521 aagatttccg taactaccca accaatactt acaaaacgct tgaacttgaa attgctgagt
     2581 ccgatgtgaa agttaaagaa gcggagcttg aactagtaaa agaggaagct aaggaacatc
     2641 gaaacgagga aaaaattaag caagtaaaag cgaaagttga gagtaaaaaa gctgaggcta
     2701 caaggctaga aaaaatcaag acagatcgta aaaaagcagc agaagctaaa cgaaaagcag
     2761 cagaagctga gaaaaaagct gctgaagcca aaccaaaagt ggatgcagaa gaatatgctc
     2821 ttgaagctaa aatcgctgag ttggaatatg aagttcagag actagaaaaa gagctcaaag
     2881 agattgatga gtctgactca gaagattatg ttaaagaagg cctctgtgct cctcttcaat
     2941 ctaaattgga taccaaaaaa gctaaactat caaaacttga agagttgagt gataagattg
     3001 atgagttaga cgctgaaatt gcaaaacttg aagatcaact taaagatgct gaaaaaaaca
     3061 ataatgtaga agactacgtt aaagaaggtt tagagaaaac tattgctgag aaaaaagctg
     3121 aattagaaaa aactgaagcg gaccttaaga aagtagctaa tgagccagaa actccagccc
     3181 cagccccagc tccagaaaaa ccagaaactc cagctgaaaa accagcgctg gccccagctc
     3241 ctaaaccaga gaagccaact gaacaaccaa aagcagaaaa accagatgat caacaagctg
     3301 aagaagacta tgctcgtaga tcagaagaag aatataatcg cttgactcaa cagcaaccgc
     3361 caaaaactga aaaaccagca caaccatcta ctccaaaaac aggctggaaa caagaaaacg
     3421 gtatgtggta cttctacaat actgatggtt caatggcgac aggatggctc caaaacaacg
     3481 gttcatggta ctatctaaac gctaatggtg caatggcgat aggatggcta caaaataatg
     3541 gctcatggta ctacctcaac gctaatggtg ctatggcaac aggttggcta gaatacaatg
     3601 gctcatggta ctacctcaac gctaatggtg ctatggcaac aggttggcta gaatacaatg
     3661 gctcatggta ctacctcaac agcaatggcg ctatggcgac aggatggctc caatacaatg
     3721 gctcatggta ctacctcaac gctaacggtt caatggcaac agattgggtg aaagatggag
     3781 atacctggta ctatcttgaa gcatcaggtg ctatgaaaac aagccaatgg ttcaaagtat
     3841 cagataaatg gtactatgtc aatggctcag gtgcccttgc agtcaacaca actgtagatg
     3901 actatagagt caatgccaat ggtgaatggg taaactaaac ctaatataac tagttaatac
     3961 tgacttcctg taagaactct ttaaagtatt ccctacaaat accatatcct ttcagtagat
     4021 acgataccct tgtaggaagt ttagattaaa aaataactct gtaatctcta gccagattta
     4081 taggctcttt gtcaactgta gtgggttgaa gactaaaatc gagaaaggac aaaatttgtc
     4141 ctttcttttt tgagtacgac gggcatgtcg tgcatctggg gtgtaagtcc tccgtgggca
     4201 cccgttaccg gtgaacccaa tagcgattcc caagtctgac tatcgtgagg tagcggggag
     4261 aggaagggat agcgaaatcg tggctctacg aacaggaaag tgatagtaag gcgtatatag
     4321 cggataaggg ggcttaaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgcgtaaa
     4381 tcacgagagt aaacgag
//
LOCUS       DAVKZB010000119         4259 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKZB010000119 DAVKZB010000000
VERSION     DAVKZB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.119"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..168
                     /locus_tag="VXA80_001771"
     CDS             <1..168
                     /locus_tag="VXA80_001771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354219.1"
                     /translation="EQTRKLAAGCSKQCFEVVDRTDEVSNHTHGKATLTWFEEIFEEY
                     KSKTFVNRDTL"
     gene            complement(444..641)
                     /locus_tag="VXA80_001772"
     CDS             complement(444..641)
                     /locus_tag="VXA80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354220.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(626..844)
                     /locus_tag="VXA80_001773"
     CDS             complement(626..844)
                     /locus_tag="VXA80_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV0354221.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(942..1727)
                     /locus_tag="VXA80_001774"
     CDS             complement(942..1727)
                     /locus_tag="VXA80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354222.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1729..2547)
                     /locus_tag="VXA80_001775"
     CDS             complement(1729..2547)
                     /locus_tag="VXA80_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV0354223.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2549..3541)
                     /locus_tag="VXA80_001776"
     CDS             complement(2549..3541)
                     /locus_tag="VXA80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354224.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1422 a          923 c          738 g         1176 t
ORIGIN      
        1 gagcaaacta ggaaactagc cgcaggttgc tcaaaacagt gttttgaggt tgtggataga
       61 actgacgaag tcagtaacca tacgcacggc aaggcgacgt tgacgtggtt tgaagagatt
      121 ttcgaagagt ataagtccaa aactttcgtt aaccgggata cattataagc acattcatac
      181 catcaaaaat agtagcaata aacttgaaca actaccagaa acacgaaaac aggcataatt
      241 gatgaaatat cagcgctatt aaacaatata aataaccgct aagaataagg cttaacggtt
      301 atcttctacc tatctatttt ttcaactcct cagtcgttta tctacctata acctaggctt
      361 tacgagtgcc ttgctctacc aactgagcta aatcggcgat tacacttaca gtatagcact
      421 ctgagaatag atgtcaagta atattattca agctttttaa tcaaaatctg acaaggattt
      481 tgcggattcc atagctgaga aaaaatctct aactttttaa agccaagacc tcgataaaag
      541 tcatttgttc gatcataatc tttattagaa ccttctgcca ctgttttgac ctgcagataa
      601 ccaacttttt tacgagcttc actctctaaa gtagcaagca attggctccc aatttttcta
      661 ccttgataag cttttttacg ccgagacaat caatctctgc acaatcttca ctcgaatagg
      721 ataaccttac aaatccagtc aaatcactct cctgataggc ggtccaaact tgcagtgtcg
      781 tggttccttc tatataggct tgtgtgcttt ctgggattcc aaaccattct ggcaaatcct
      841 tcaaaacctc agcgacaact gccttttttg attttcatcc ttgacttctt caattacaaa
      901 catcataagt tcctttcttg attttagtct tactttagct tttacgaacc cgcactttcg
      961 taggaatcta agcccttatc ccaaagttta agggaaataa caaagaaaac cagagaaatc
     1021 aacatcaaac ctcctacgtt aaagaacaca tccttttcct gtaagaaata gctagctgga
     1081 tagtaggctg taaaggcgaa aggcacgata aagctaatca accaacgaag aagagaattg
     1141 taaatagaaa tcggatactt agcaaagtca ttgaacatat agaagatgta aatcatggcg
     1201 cctgactgct tagtccaaaa ggcgatactg gctgttgcga ttttaagaga agtataaatc
     1261 aaggtcgcaa aaggaataca aactaggaaa agcaggaatt ttggaagagt ccaaacaatg
     1321 ctggtcactg ttgttcccaa taaaatacca ccgactaaga gttcacccaa ggcatcaatc
     1381 tgaaaggttt caactaggat atgaaagaga ggattgatgg gacgagtcag atacttgtca
     1441 aactcccctt ttcggactag gcgttgccct agtgcccaga gattgtcaaa aaagagatgg
     1501 tccattccct tgggaatcaa ggaaaatcca taaatgaaag ctatctcttg aaaggtccag
     1561 ccttctagga atggaatatg ttgaaagatg acattgagaa acaagagatt caagccttga
     1621 gccagaaaga ctcccaagac accaaccaca aaatccacct tatattccat gatttgtttg
     1681 atgtattgtc tgataaaaat cagatgcatt cgttgatatt ttttcatact aacctccttg
     1741 aatggtgata aaggactgga cccgtttcca aatcaactga gacaatccca ccatcactaa
     1801 gagccagaag aactgcaaaa ggagtgcctg aagaatctga ctggcatcgt attttccaac
     1861 aatgatcata actggagtat aaatcaagga tgaaaaaggc aaaaaggaga gaatatctga
     1921 aacaaccttt ggaaagaatg ccaagggaat caaactcccc gacataaaag ccactatgga
     1981 agtcttaagt aggttggaac cccaaagatt tttaaacaca aaggctgaaa atccaaagca
     2041 aatattaaag aaaaagttaa tcagataggc gagcgttaag ctaaaaagat aaatgacagt
     2101 taatcctagc acctctacaa taccttgacc cgatatgatt ttcatcaaga caatgacact
     2161 taaaaatgga aggccaacgc tgataaaaat caaccacttg gaaccaagct cggtgaaaag
     2221 ataggaggcc gcaaaatgca ctggtcgcaa caaacgcatg ataatggagc catccttgac
     2281 ctcctcccca atcataaagg acgaatcgga tctagtcaga agattggtca caaaactcat
     2341 gatgatgtag agggtgatat ccgccatact gaagccctga atcaaagact cttgcgaaga
     2401 atcaaagaca gccttccaga gataaaaggc cacaaaagcc cccatgacat cgccaatccg
     2461 atagagaata aagttgactc ggtaagtaat caactcctga acccctgcat tgataaaggg
     2521 tttataacgt ctccacaatt tgatcatcct agagctcctt tcggtagaag cgacggataa
     2581 tatcctcaat atccgtatcc accatcttca aatcgcggat ttcaaaatca gacagggttt
     2641 gcttgataat gtcagctgac tggtagcgag aactatcaaa ttcaatgttg aggctgtttc
     2701 cttgtctatc aatggtcata tcagacagac cttcatagtg agagacgaga tgactttgac
     2761 ctggtagcag ttcaaaagag agagtcttca tcttaccaaa ggtctccttg agttggctca
     2821 ccgttccatc aaaaatctct tgccccttgt caatcatgaa aatccgatca caaagttgct
     2881 caatatcact caaatcgtga gtggtcaaaa gaatggtagt ttcttcctct tgattgatct
     2941 gagtaattgc ccgacgaata ttatccttaa ccgaaacgtc caaaccaatg gtcggctcat
     3001 ctaaaaaaag aaccttggga ttgtggagca aggaggccgc aatatccgcc cgcatccgtt
     3061 gtcccagtga aagagtccgc acgggatcct tgataaagtc cttcaaatcc aagacttcat
     3121 tcaaaaagtc catacgctta tgaaagagcg agtctggcac atcataaatc tcttttaaga
     3181 cagtgtaggt ctcttgcaga gccaaatccc accatagctg ggtgcgttgt ccaaagacta
     3241 cgccaatatc tttgacataa tcttgccgat tgttctgggg aatcttgccg ttaatccgac
     3301 aaaaaccaga tgttggtttc aaaattcctg tcagcatttt aatggttgtc gacttcccag
     3361 caccatttgc cccgataaat cctaaaatct gcccttttgg aacctcaaag gtcaaatcct
     3421 tgaccgcttc aaaggtctgc ttttcaggat gaataaagga gcgcaaagcc cccttcaagc
     3481 ccggttcctt aacagtcttc acaaaatttt tctgaagatg ttccacttct atcattgcca
     3541 tatctatctc cctatcgtaa ggaatagcaa cattgtgttt ttaactacaa atattctaaa
     3601 tccttacttt ctacaccttc tacattttat tactacagaa ggctttatac caacctatta
     3661 tagtccaata aaaaacagaa tgcaagcgtt tgcctatgga ttgtgaaatt aggatttctc
     3721 tcatgaaata atacttttaa tcaaaaattc tgtttaatag cttaaattac caacctactc
     3781 atcttctcac cagagggcgg tattaacttt gatcataaaa tcggatactc cctctatcca
     3841 gaaaatatgc tcagaatgat tgtctctaaa caatacctgc tcttttacct cattcatgaa
     3901 aaagaagtcc ataccctgag aattatcaac tcacgcaccg actacctaaa ccaactcgac
     3961 cacctctttc gtacatgtag gaaactattt tcctcacaaa taatctcatt ataaaatcca
     4021 ataaattttt tagctcactt gaatattttc tttgtatcgt tttatcagct tcaatctgat
     4081 ctagacttgt taccttggaa cgacttgaaa taacctttat aaaataacac ttaagacttt
     4141 catcatttcg tataaatttt tcaatagtga tataaaaagc tttcatattc tgtttgtaat
     4201 cagatatcaa ttgattaatt ttatcattct gatgaagatt cgataccata atatatagc
//
LOCUS       DAVKZB010000120         4235 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKZB010000120 DAVKZB010000000
VERSION     DAVKZB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.120"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(17..568)
                     /locus_tag="VXA80_001777"
     CDS             complement(17..568)
                     /locus_tag="VXA80_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354225.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(918..1742)
                     /gene="nadE"
                     /locus_tag="VXA80_001778"
     CDS             complement(918..1742)
                     /gene="nadE"
                     /locus_tag="VXA80_001778"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV0354226.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1739..3199)
                     /locus_tag="VXA80_001779"
     CDS             complement(1739..3199)
                     /locus_tag="VXA80_001779"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV0354227.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3315..3803)
                     /locus_tag="VXA80_001780"
     CDS             complement(3315..3803)
                     /locus_tag="VXA80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354228.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGATYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(3793..4002)
                     /locus_tag="VXA80_001781"
     CDS             complement(3793..4002)
                     /locus_tag="VXA80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354229.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1184 a         1003 c          796 g         1252 t
ORIGIN      
        1 aaacagaaaa tagaattcaa cattcttccc tacttatacc ataataaata agatccgcaa
       61 gatagccttt tctctctaca ccattaacaa tggtttgtag ataggacatt ccagcctttt
      121 ccatgacacg acctgaagct gggttgagac tggcataacg tgctctgacc ttttgaaaac
      181 ctgcttgagt aaaacaaaag tccaagatag ctttcaaggc ctctgtcatc ataccatgtc
      241 cccagtaagc cttgcctagc acatagccga tttcacagct taaatcagcc tcgtctatct
      301 taacaatgct gatatctcct atcacttgct ctgggttttc ttttagacaa atggcccatt
      361 tataatagtt gagattagta taggaagcaa cccaattgcg aatcgagttt cgagtgattt
      421 cgatatcagg atggggatcc caggtaacat aggtcagatt ctcagcggat gaagcccaat
      481 tttgaaacat agcttctgca tcactctcca caaatcttct taaaatcaaa cgttctgtct
      541 gtaatatttg cgtaccaata gatttcatga tgctacctca cttccttatt tctatgctcg
      601 tagtaaaaag gctcaactca gtaacagcta gaaaacgagt ttgtcttcta atttcttgac
      661 gcaatcgctg cctggtttga aatgcgatct agcaagcttt ttcaaaccta gtcatcgttg
      721 caggggtaag acgatgaaat cgaactcttc gagttaccga atctgcctca ttttcttatt
      781 tctaaactcg gggttcaaga tcccccagac cttctctcac tttcttattt taagatgaga
      841 ggctgaaaac ctccagtgga ggtttttctt gctttttaat ttcagggcaa gaagctaaaa
      901 aggtcccccg gaccttttta ctcccaaaag tcatcaaata cagtgatggg taagtggcgt
      961 ttgtgttggc ctttgtgcca ccagttttca atggtcgctt gagcttctgg gctgattgtt
     1021 ttgccttcta ggtagtcgtc aatctctgcg taggtgactc caagtgcgac ttcgtcagct
     1081 aggcctggtt tatcttcttc taggtctgcc gttgggattt tttcataaag ggctggctct
     1141 gcgccaagtt tctgcaagag ttgttttcct tggcgtttat tgaggcggta aagagggaga
     1201 atatccgcac cgccgtcacc aaacttggta aagaaacctg tgatattttc cgcggcgtgg
     1261 tctgttccaa tgaccgctcc gctatgggaa ccagcaaggg catactgagc aatcatacgg
     1321 caacgtgcct tgatattccc cttgttgaag tctgaaacag gactacctgt cgcttcaact
     1381 gcagctgtca tggcatcagc tgattccttg atattcacaa ccaagctgac atctggctgg
     1441 atgaaggcta gggctttttg agcatctgct tcatcagctt gcactccgta tggcaggcgg
     1501 acagcgataa atttgtagct atcgtctccc gtttcagctc gcagttcttc catagctaat
     1561 tgcgccaaac gtcctgccaa ggttgaatct tgtcccccag aaatccctag tacaaaggtt
     1621 tttaggaagg gatgtttttt cagatatctt tttaagaaat caatagaacg acggatttct
     1681 tcctgggcat caatcactgg tttgacaccc agctcttgga taatcgtttc ttgcaaactc
     1741 attcttcttc tccttcacca agggcttcct tgcgcatctt atcaatcaag tccatcttat
     1801 cttgccatac atcacgcgcc aaatccactg gatagtgctg cggattgagc acacgcttat
     1861 actcatccca caacttgtca aattccttac gggcataatc ctgaatgtca gtcaaactag
     1921 gcaagttgta aactaatata ccttctttga agatatccac caagagagga acggcatcaa
     1981 aattacgaac cgtcttcttg atgtatgtat aggtcggatg gaacatcttg atttctgtca
     2041 tgtcgctaat atccacacca tcataagtga tgtagtcacc ttctgacttg cctttttcac
     2101 gactggtaat gcgccacacc tgcttcttac ctggcgtcga cactttttcc gcattattag
     2161 acagcttaat cgtattgcgc atctgaccag tttcatcttc gattgcaaca atcttgtaaa
     2221 ccgccccaag agctggctgg tcataggctg taatcagctt ggtacccacg ccccagacat
     2281 caatcttggc cttttgcatc ttgaggttga ggatagtatt ttcgtccaaa tcattagaag
     2341 cataaatctt agcctctgta aatccagcct cgtccagttg ctgacggact ttcttagaaa
     2401 tgtaggcaat atccccagag tcaatccgca cacccataaa gttaatctga tcacccagct
     2461 cacgcgccac ctgaatggca gctggtacac cgatgcgaag ggtgtcatag gtatccacaa
     2521 gaaagacaca atttttgtgg gtcgcagcgt aagccttgaa agcttcatag tcattgccat
     2581 aaacctgtac caaggcatgg gcatgggttc ccaaaacagg aatgtcaaag agcttacccg
     2641 cacgcacgtt gctggttcca ttggcgccac caatcacagc tgcgcgtgtt ccccagatgg
     2701 ccgcatccat ttcttgagcc cgacgtgtcc caaactccat caagggttca tcttcgataa
     2761 ccgaacgaat acgagctgcc ttcgtcgcca ccaaggtctg gtagttgacg atgttcaaaa
     2821 gagccgtttc gaccaactga cattgggcta gaggtccttc cacctgcaca atcggttcat
     2881 tagcaaaaac caaatcccct tcttgggcag aacgaacggt caactccaac ttgaaattgc
     2941 gaaggtaatc caagaacgcc ccatgataac caagcgactc caaataggct atatcactat
     3001 ctgaaaaacg caagtcttca agatagttca caattctttc caaacctgca aaaaccgcat
     3061 agccgttctt aaaaggctgt tggcggaaat acacctcaaa gaccgccttc ttattgtgaa
     3121 tcccttggtc aaagtaaacc tgcatcatgt tgatctggta caagtccgtg tgcaatgtca
     3181 aactatcatc tggatacata cttttcctac ttccttagct agaaacccat gaaaattttc
     3241 aagaactttc atgtattcca ataaattagt actattatat cacattttag ctggattgag
     3301 aaaagagtaa caagctattc tccactctcc aattcatcca tatcttgttc aaattttttc
     3361 tgagcccatt cgccatagct cttaagacca agattgccaa taaagaccca cggaaggtaa
     3421 atgacataag taatgaccca agcagacagg tatttaaaat tcaaaggatt gtgctgataa
     3481 atttctatgt tgaattgata attctgcaac atcaaaagag ccgtaatagc caaggttagg
     3541 aaaaaacaac ccaaaatcgt aaaatgaaaa cgactatagt aggtcgctcc cagataacgg
     3601 gcacgattga aaaagtaaaa tgtccctatg atgataacga ttagcagcat attagaatta
     3661 aaaaggcttg gtgctaatac tgaaatgata taagatagga gcgacaaagc aatgcagata
     3721 tagaaacttt cagagcccgc tttattgaac agttgttctt ctctttcgtc tagtaattga
     3781 taataataaa atctattttt catcttcttc ctcccaaaat agttggtcta gggttttccc
     3841 taaacatctg caaatagact ggcagagcga gagactggga ttgtattttc ccgcctctat
     3901 caaaccaata gtctggcgtg tcaccccgac agcctctgcc agttgacctt gtgttaaatc
     3961 acgctctacc cgagctaatt ttaattttaa atttttagcc accttcgtcc tccttatagt
     4021 tttaatactc atctactctt aaaaaatcca aaaccaacac aagctatcag ctatttatta
     4081 taaccccatt aatttgcact ccagtattat cttcagccga aaatcagtat tttaggttgt
     4141 ttgaaataaa cttcctagtt tactctttta tttttattta gtattagctt tagtaggatc
     4201 ttttaaacga gtttttattg cttatatttc ttgtt
//
LOCUS       DAVKZB010000121         4176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKZB010000121 DAVKZB010000000
VERSION     DAVKZB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.121"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VXA80_001782"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VXA80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV0354230.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1320..2024
                     /locus_tag="VXA80_001783"
     CDS             1320..2024
                     /locus_tag="VXA80_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354231.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2017..3366
                     /gene="vicK"
                     /locus_tag="VXA80_001784"
     CDS             2017..3366
                     /gene="vicK"
                     /locus_tag="VXA80_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV0354232.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3368..>4176
                     /locus_tag="VXA80_001785"
     CDS             3368..>4176
                     /locus_tag="VXA80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0354233.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT         1253 a          743 c          968 g         1212 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gtctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggtccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttt ggtataatag tagagaaaaa ggtgaacata
     1321 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
     1381 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagca ctagagcaat
     1441 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
     1501 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
     1561 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
     1621 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
     1681 ctatgccagt agatggtcag gaagcagata gtaaacctca acctatccaa attggggatt
     1741 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
     1801 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
     1861 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
     1921 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
     1981 tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
     2041 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
     2101 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
     2161 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
     2221 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
     2281 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
     2341 gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
     2401 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
     2461 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatatc
     2521 aatggtgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
     2581 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
     2641 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
     2701 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
     2761 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
     2821 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
     2881 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
     2941 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
     3001 acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga tgggggtaaa
     3061 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
     3121 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
     3181 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatc
     3241 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
     3301 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
     3361 gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt ccagtggaaa
     3421 ttctttttat ctggaaacct caaaaaagaa gcttttagta gatgcaggct tgtctggcaa
     3481 gaaaattacc agtctgctag ctgaaattaa ccgtaagcca gaagacctgg atgccatctt
     3541 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
     3601 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
     3661 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cctttggaga
     3721 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
     3781 tatgaaggat gataagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
     3841 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
     3901 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttggtca
     3961 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gtactaagaa
     4021 gatttacctt gggcatttat ctaaggaaaa caatatcaag gaactggctc atatgaccat
     4081 ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg tttatgatac
     4141 ctcaccagat accgcaacac cattgacaga gatata
//
LOCUS       DAVKZB010000122         4162 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKZB010000122 DAVKZB010000000
VERSION     DAVKZB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.122"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(42..113)
                     /locus_tag="VXA80_001786"
     tRNA            complement(42..113)
                     /locus_tag="VXA80_001786"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Arg,seq:cct)
     gene            complement(156..503)
                     /gene="rplS"
                     /locus_tag="VXA80_001787"
     CDS             complement(156..503)
                     /gene="rplS"
                     /locus_tag="VXA80_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV0354234.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(622..951)
                     /gene="crcB"
                     /locus_tag="VXA80_001788"
     CDS             complement(622..951)
                     /gene="crcB"
                     /locus_tag="VXA80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0354235.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(945..1319)
                     /gene="crcB"
                     /locus_tag="VXA80_001789"
     CDS             complement(945..1319)
                     /gene="crcB"
                     /locus_tag="VXA80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0354236.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1316..1582)
                     /locus_tag="VXA80_001790"
     CDS             complement(1316..1582)
                     /locus_tag="VXA80_001790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV0354237.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1698..2141)
                     /locus_tag="VXA80_001791"
     CDS             complement(1698..2141)
                     /locus_tag="VXA80_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV0354238.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2245..3180
                     /locus_tag="VXA80_001792"
     CDS             2245..3180
                     /locus_tag="VXA80_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV0354239.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3234..3518
                     /locus_tag="VXA80_001793"
     CDS             3234..3518
                     /locus_tag="VXA80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV0354240.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3575..3793)
                     /locus_tag="VXA80_001794"
     CDS             complement(3575..3793)
                     /locus_tag="VXA80_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354241.1"
                     /translation="MSISPRFETLEQAIAFKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3790..>4162)
                     /locus_tag="VXA80_001795"
     CDS             complement(3790..>4162)
                     /locus_tag="VXA80_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354242.1"
                     /translation="GESFFLIAGRCNTEWMNAFLEELSQAYPDDYLLFVMDHAIWHKS
                     STLKIPTNIGFTFIPPYTPEMNPIEQVWKEIRKRGFKNKAFRTLEDIMNQLQDVIQGL
                     EKEVIKSIVNRRWTRMLCESR"
BASE COUNT         1292 a          924 c          754 g         1192 t
ORIGIN      
        1 acattttgaa ttttctttgt ctttctttac attactaaaa atgccccctg caggaatcga
       61 acctgcaact actccttagg agggagttgt tatatccatt gaactaaggg agctagataa
      121 aaactctgct gaaaaagcag agttttttag tcgaattaac gacggatttc tttgatacga
      181 gctgccttac cttgaagagc acgcaagtag tacaatttcg cacgacgtac tttaccgtaa
      241 cgaacaactt cgattttttc aacacgtgga gtgtggattg ggaagatacg ctcaacacct
      301 acaccgttag agattttacg aactgtgtag ttttctgaga ttccagcacc tttacgtgcg
      361 ataacaacac cttcaaaaat ctggatacgt tcacggttac cttcgacaac tttcgcatgt
      421 acacgaacag tgtcaccagg acggaatgat gggatatctg tacgaagttg accttcagtc
      481 aagctttgga ttaatggatt cattttattc tcctatcttt gtcaatcttg aggaaacttc
      541 ctcagcggat aaactgtatt tttgtgcgtc catcacacac aagatacagt ctaccaaatt
      601 ttcacaaaaa agtaaagaaa tttatagcaa aattcctaaa aaaatcgcaa ccaaaccacc
      661 tatgtaagtc aaagtcaggt aagaataaaa gaccttctta tcacttagca gtctttgaag
      721 ttcgtcattc aaggtcgaaa aagttgttaa accgccacaa aatcctgttg ctagaatagc
      781 atatacttcc ttagactcta catgattgta gaatactcca attaaaaaac aacctaaaag
      841 attagctatg agcgttccga gtggcaattt agaagcttga ttgtagcggg aaaagaaata
      901 ccgtacgagt gctcccaagc cacaggcgat tgcaagatag acgattacca tttcttcctc
      961 cctagaaaat aagctaagag taggcctcca ccgatgctca aaagcaaata cagtatcaaa
     1021 ctaagataac gccctgtatc aagcagcttc acagtatcaa gcattaggct ggaaaaagtt
     1081 gttaaacctc cgcaaaaacc cgtccccagt gctaaaatca agcccttact agtcccctta
     1141 tagaccagat agcccttgac aagataaatc aagcagaaaa ttcccagata gttgacaaag
     1201 agagtgcccc aagggaaatc tggactggct ggtaaccaag tggaaactag gtagcggaca
     1261 agtccgccca acatagcagc tagaaaaatc cctagcggat aaaattgttc ttttttcatt
     1321 tgatattttg atcctgataa tcacgcgaac atttgagtat gtccgaaaaa gtcgcgacaa
     1381 tagtctcctg atagcgcttg tcctctacac gacttctaac cttttcaaaa ataactgctt
     1441 ctctcttagt atctaaaatc ggtttccctg aagccttctt ataagcgaca actccttcaa
     1501 ctaagtgcat tcgttcttct aacagtttga caatttggtc gtcgatttga tcgatttctt
     1561 gacgaataat atctaaatcc atacagtctc ctcctttatt tgaattattg tatcaaaaag
     1621 ccatcaatta ggctagtaaa atcccaactc tcgataagaa aaatgcactg attgattaca
     1681 tcagcgcact tttatgctta tcctacttta gcaaccaatt cttctgcgaa ttgttccaag
     1741 cgttcaatat cttcttcctc ggcagaaaga tcaactttaa cacactctga acctttttct
     1801 gctcctgttg acacaaagac acggtcaaag tcatcaacag ccttacagaa ttcatcgtag
     1861 aaggtatctc ctgagccgac cactccgtag attttgccat tcaagttgag atctgctagg
     1921 tcttcgtaga agtccatcat ctcatctggc aattcaccat caccataagt ataggtcgca
     1981 acgatagcga tgtctgcttc caagaagtct gaagcgtcaa cagttgtaca ttcatcaaca
     2041 tcaacatcca agcccaagtc acgtaatttg tctgctacaa tatctgcaat ttcttcggta
     2101 ttaccggtca tactggcaaa tacaattttt gctaatgcca tatcgtcctc ctcaatttat
     2161 ctttctccat tatatcacat ctttttcatt ttaaaaataa aaattcttgt gctacaatga
     2221 aatgagaaag aattgaggtt atttatggag atttgccaac aaattttaga aaaaattaaa
     2281 gaatacgaca ccattatcat tcatcgtcat atgaaaccag accctgatgc cttgggaagt
     2341 caggtgggat tgaaagcctt gctagaacat catttcccag aaaaaaccat caaagccgtc
     2401 ggttttgatg aaccaactct tacttggatg gctgagatgg atcttgttga agatagagcc
     2461 taccaaggcg cacttgtcat cgtctgtgat acagctaata ctgctcgtat cgatgataag
     2521 cgctatagtc aaggtgattt tctcattaag attgaccacc atccaaatga tgatgtatac
     2581 ggtgacctgt cttgggtcga tactagttca agtagcgcta gcgagatgat taccctattt
     2641 gcccaaacaa cccaactagc cttggcagat cgcgatgctg agttgctctt tgcaggaatt
     2701 gtcggtgata caggtcgctt cctctaccct tctaccactg cacggactct tcgcctggct
     2761 gcttatttga gagaacataa ctttgacttt gcagctctca ctcgcaaaat ggacactatg
     2821 agctacaaaa ttgctaaact gcaaggctac atctacgacc atctggaagt ggatgaaaat
     2881 ggtgctgctc gcgttatcct gagtcaagaa gtcttgaaac aatacaatgt aaccgatgct
     2941 gaaactgcag ccattgtcgg tgcacctgga cgcattgaca gagtgagtct ctggggaatt
     3001 tttgtcgaac aggctgatgg ccactaccga gttcgcttac gcagtaaaat ccatcctatc
     3061 aatgaaatcg ccaaggagca tgatggtgga ggccaccctc tagcaagtgg tgctaattcc
     3121 tatagcctag aagaaaatga aatcatctac caaaagttaa aaaacttgct taaaaactga
     3181 taaaatactt gccaaacttt tcagaatctg atagactagt atggtaacaa tctatggctc
     3241 gcaaagagac catggcagaa aggaaatatt gcaaaatgaa aaaagatatc catccagaat
     3301 atcgcccagt tgtcttcatg gacacaacta ctggttacca attccttagc ggttcaacaa
     3361 aacgctctaa cgaaacagtt gagttcgaag gcgaaactta cccattgatc cgtgtggaaa
     3421 tttcatcaga ctcacaccca ttctacactg gacgtcaaaa gttcactcaa gcagatggac
     3481 gcgtggatcg tttcaacaaa aaatacggtc tcaaataatg ataaaagaac agtttcgact
     3541 gttctttttt gtttcttgaa atcaactgct gttttcatgt tccagactca tctgtaggtt
     3601 cggtttccat gctactaggc aggaaggaaa tagctgtttc aacacgtcca taatgagctg
     3661 tactattgtc acgaaccaca ctttcattga tggtccaagt ggaattcatt ttcttaaaag
     3721 cttctcggac tttttccaaa tctttgaagg caatggcctg ctctaaggtt tcaaaacgag
     3781 gacttatact catctgcttt cacaaagcat tctagtccat ctccgattaa cgatggactt
     3841 tatcacctcc ttctccagtc cttgtatgac atcttgaagt tgattcatga tatcttccaa
     3901 agttcgaaag gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat
     3961 ggggttcatc tctggtgtgt atggaggaat aaaggtaaaa ccaatattag tcggaatctt
     4021 taaggtactt gatttatgcc atatagcatg gtccataacg aataaaagat aatcatctgg
     4081 ataagcttgt gaaagctctt ctaaaaaggc gttcatccac tcagtattac atctaccagc
     4141 tattaagaaa aatgattcgc ct
//
LOCUS       DAVKZB010000123         4085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKZB010000123 DAVKZB010000000
VERSION     DAVKZB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.123"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            170..370
                     /locus_tag="VXA80_001796"
     CDS             170..370
                     /locus_tag="VXA80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354243.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            395..811
                     /locus_tag="VXA80_001797"
     CDS             395..811
                     /locus_tag="VXA80_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354244.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1042..1749
                     /gene="ccdA2"
                     /locus_tag="VXA80_001798"
     CDS             1042..1749
                     /gene="ccdA2"
                     /locus_tag="VXA80_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0354245.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1760..2317
                     /gene="sdbB"
                     /locus_tag="VXA80_001799"
     CDS             1760..2317
                     /gene="sdbB"
                     /locus_tag="VXA80_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV0354246.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2691..4082
                     /locus_tag="VXA80_001800"
     CDS             2691..4082
                     /locus_tag="VXA80_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV0354247.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASAWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT         1189 a          742 c          861 g         1293 t
ORIGIN      
        1 gtaaaatgaa ataagaatag gataaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagt ccatatgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc
      121 tgcaagtcct gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagtaag
      181 cgcttcggct agctcaacga gcccacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagttag accaacactt gaaagctttg gcaacagtat ctgatccgaa
      301 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa
      421 agatgtgaaa gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca
      481 aggaacggaa gatagtaagg attcagataa gatgactgaa acaaactcag ttccagcagg
      541 agtgattgtg gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg
      601 taagaaagag tcagaaatcc agcaattaag cacggaatta atcaaggttc taggacagct
      661 agatgcagaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac
      721 ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga
      781 gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacttaaa
      841 agacattttc gttcacaaaa gcagcgacga gtaatccttg tcgttgcttt ttggctattt
      901 tgggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac
      961 gattttgcgt agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt
     1021 attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca
     1081 ggtgttttat cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt
     1141 ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg
     1201 tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta
     1261 ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg atatgtcatg
     1321 ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta
     1381 gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt
     1441 ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct
     1501 gttttagcac ttgcggcttc tggaggcaat ggtgcttggc aaggagcgat ttatactctc
     1561 atttacactc tgggcatggc ccttcctttc ttggtcttgg cactagcttc aggtctagtc
     1621 atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc
     1681 ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt
     1741 tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt
     1801 agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc
     1861 aaaacaacaa acgactgtac aacaaatttc tgttggaaaa gatgctccag actttacatt
     1921 gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt
     1981 gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact
     2041 agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg
     2101 tgaaaaaact gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc
     2161 agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac
     2221 agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa
     2281 tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag
     2341 atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa cctagctttt
     2401 agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt
     2461 tctttttctt gatatataag ggttcaaaag cgaaaaaact cagaaaaagt gcacgacaaa
     2521 tagccctaaa acagagtcgt cttagagtaa cttccagttt tgggagagag atggaagtta
     2581 ctttgagaag ttacggtagt cagatttttt tagaaaaaca ttttataaat attcacatat
     2641 ctcctatatt tatggtaaaa tagaattatc agtttatttt ggagtcaaag atgaatatat
     2701 ttagaacaaa gaatgttagt ttagataaaa cagagatgca taggcatttg aagttatggg
     2761 atttgatttt gctgggtatc ggagccatgg tagggacagg cgtctttaca atcacaggta
     2821 ctgcagctgc aacacttgct ggcccagccc tagtgatttc aatcgttatt tctgccttgt
     2881 gtgtgggatt atcagccctc ttttttgcag aatttgcctc gcgagtaccc gctacaggag
     2941 gtgcctatag ttacctctat gctatcttag gagaattccc tgcctggttg gctggttggt
     3001 taaccatgat ggagttcatg acagccatat caggcgtagc ttcggcttgg gcagcttatt
     3061 ttaaaggctt gctctctcaa tacgggatag cctttcctca ggctttaaat ggtaccttta
     3121 atcctcaagc agggacattt gttgatttat tgcctattct tgtcttggtc ttggtgactt
     3181 cgcttgtttt actaaatgcc aaggcagcct tacgctttaa ttcgattcta gttattttga
     3241 aattttccgc tttagctctc tttgttttag taggaatttg gaatattaag tttgataatt
     3301 ggagcaattt tgctccttac ggttttggac aaatctatgg agctagtact ggtattatgg
     3361 ctggtgcatc cttgatgttc ttcggttttt tgggatttga atccatctct atggctgtag
     3421 atgaagtcaa gactcctcaa aaaaatattc ctagagggat tgtcttatcg ctttctatcg
     3481 taaccattct ctatgccttg gtgacccttg ttttgactgg tgtggttcac tatagtcatt
     3541 taaatgtcga tgatgccgtt gcctttgccc ttcgtagtgt tgggattagt tgggcagcca
     3601 actatgtgtc attagtggct atcttgacct tgattacagt ttgtatttcg atgacctatg
     3661 ccctatcgcg tatgatttac agtttagcac gtgacggctt agtgcctgct gcctttaagg
     3721 aattgacgaa gactagcaag gtaccaaaga atgctactat cttgacaggt ctagcttcag
     3781 cagtagcagc aggaatgttc ccactagcca gtattgcagc ctttttaaat atttgtacct
     3841 tagcctactt gatcatgttg gcttatggtc tgattcgctt acggaaagaa aaaggaatgc
     3901 ccaaagctgg ggaatttaaa acaccattgg tacctctgtt accaatttta tcgatcatca
     3961 tttgtttatc cttcatgtta cagtataata tgaatacctg gattgctttc ctagttgcgt
     4021 tgttagtagg aagtattatt tacttcactt atggctataa gcattctacc atagaagaat
     4081 aaagt
//
LOCUS       DAVKZB010000124         4064 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKZB010000124 DAVKZB010000000
VERSION     DAVKZB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.124"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            13..966
                     /locus_tag="VXA80_001801"
     CDS             13..966
                     /locus_tag="VXA80_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0354248.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1043..3031
                     /gene="uvrB"
                     /locus_tag="VXA80_001802"
     CDS             1043..3031
                     /gene="uvrB"
                     /locus_tag="VXA80_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV0354249.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            3041..3604
                     /locus_tag="VXA80_001803"
     CDS             3041..3604
                     /locus_tag="VXA80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354250.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3570..4058
                     /locus_tag="VXA80_001804"
     CDS             3570..4058
                     /locus_tag="VXA80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354251.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQMWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
BASE COUNT         1192 a          713 c          939 g         1220 t
ORIGIN      
        1 gaggacgggg atatgaagaa caaaagaata tttaaagact tccaagcttc aaaaatgagt
       61 ttaaacattt acacaagccc cttgttagcc tttgtttttg tcttcatagg agagtttgtg
      121 gcttttactt tgtatggtat tggcttgtta gctctcatcg gacttgctag aaattttgga
      181 gaggctggtc aaaatcttgc aacctacttg cagaccttgc atcagagctt gacggataaa
      241 acaagtgact ttcgtttaat tttaggatta ctggcctttg gttttattct taacactgtg
      301 ttcagatgga caagaaaagt tgagaaaaga cctattcgaa ccttgggatt ttatagagag
      361 aatttcctca gcaatcttct gaaaggattt agtctaggct tggcactttt tcttctgacc
      421 ttgttaggtt tagtggtctt aggtcaatat cgtttggaat ccattcactt gaatccttat
      481 tctcttgcct ttgtcgtctt tactatccca ttttggattt tacaggggac agcagaagaa
      541 gtggtggccc gtgcttggct acttcctcaa ttggcctcaa gaaccaatct aaaactagct
      601 attcttatat ctagcctgtt ctttaccctg cttcatatgg gcaattctgg cctcacccct
      661 ctatctctag taaatctctt tttattcgga gttgccatgg ctctttatct tctcaaaact
      721 gatacagttt ggggtgttgc aggtattcat ggtgcttgga attttgctca gggtaatctc
      781 tttgggattt tagttagtgg tcaaccgtca ggaacgtctc tgatgacctt tttaccacaa
      841 ggcaatcaag attggctatc aggtggttct tttggcatag aaggttccat tatgacaagt
      901 ctggtattac tactgctgat tgtctatctt gctaataaat taaagaaaga aaatgaaagg
      961 atgtgacttc ggtccgtcct tttcttcgtg aaaatactat aagtatgcta aaataggaat
     1021 agcacatgga gagaggattc ttatgatcaa tcacattaca gataatcaat ttaaactagt
     1081 atcaaaatat caaccatcag gagatcaacc ccaagctatc gagcagttgg tggataacat
     1141 tgagggggga gaaaaagctc agattctgat gggggcgact ggaacaggga agacctatac
     1201 tatgagtcag gtcatttcta aagtcaataa accaactctg gttattgccc acaataaaac
     1261 tctggctggt cagctctatg gggagtttaa ggaatttttc cctgaaaatg cagttgagta
     1321 tttcgtatcc tactatgatt attaccagcc agaggcctat gtcccttcta gcgataccta
     1381 tattgagaag gatagttctg tcaatgacga gattgacaag cttcgccact cagctacctc
     1441 agcccttttg gagcgtaatg atgttattgt cgtggcctca gtctcttgta tctatggttt
     1501 gggttcgccc aaggaatacg ctgatagtgt cgttagtctc cgtcctggtc tagagatttc
     1561 tcgtgataaa ctcttgaatg acttggtcga tattcagttt gaacgtaatg atattgattt
     1621 ccaacgcgga agatttcgcg ttcgtgggga tgtggtagag attttcccag cttcccgaga
     1681 tgaacatgcc tttcgagtag aattttttgg agacgaaatt gaccgtattc gtgaagttga
     1741 ggctctgaca ggtcaggtgt tgggagaagt ggatcattta gcgattttcc cagcgacaca
     1801 ctttgtgacc aatgacgacc acatggaagt tgccattgca aagattcagg ccgagttgga
     1861 agaacaatta gctgtctttg aaaaggaagg taaactgctt gaagcccagc gtttgaaaca
     1921 gcggacagag tatgatatcg aaatgttgcg tgagatgggc tataccaatg gggttgagaa
     1981 ctattcacga cacatggatg gacggagcga aggagagcct ccttatacgc tcttagattt
     2041 cttcccagat gatttcttaa ttatgattga cgagagtcac atgaccatgg ggcaaatcaa
     2101 gggcatgtac aatggagacc gttcgcgtaa agaaatgctg gttaattatg gcttccgatt
     2161 gccgtctgct ttggacaatc gtcctctccg tcgggaggag tttgagagtc acgttcatca
     2221 gattgtttac gtttcagcga cacctggtga ctatgaaaat gaacagactg agacagtgat
     2281 tgagcaaatc attcgtccaa cgggactctt ggatcctgaa gtggaagtcc gtccgactat
     2341 gggacagatt gatgacctct tgggtgaaat caatgcccgc gttgaaaaaa atgagcgtac
     2401 ctttatcaca actttgacca agaaaatggc agaggacttg accgactact tcaaggaaat
     2461 gggtatcaag gtcaagtaca tgcactcgga tatcaagacc ttggaacgga cggagattat
     2521 ccgtgacctg cgcttgggtg tctttgatgt cttggtcgga attaacctgc tccgtgaagg
     2581 aattgacgtt cctgaagtga gcctcgtagc tattctcgat gctgataagg aaggtttcct
     2641 tcgcaacgaa cgtggactca tccagactat tggacgtgct gctcgtaaca gtgaaggcca
     2701 tgtcatcatg tatgcagaca cggttaccca gtctatgcaa cgtgctatcg atgaaactgc
     2761 ccgccgtcgg aaaattcaga tgacctataa tgaagagcat gatatcgtac cacagacaat
     2821 caagaaagaa atccgtgacc tgattgccgt gaccaaggca gttgctaagg aagaggacaa
     2881 ggaagtcgac atcaatagcc tcaacaaaca agagcgcaag gaactcgtca agaaactaga
     2941 aaaacaaatg caagaagccg tcgaagtgct tgactttgaa ctggcggctc agattcgtga
     3001 tatgatgttg gaagtcaagg tcttggatta ggggaatagt atgatttatt taagaaagtt
     3061 aaagagagaa gatttgatgt ctttatggga aatggcttat tcacaactta atccagtttg
     3121 gaaacagtat gatgctccct attatgatga ttatcagtat ttttcaaatt ttaaagaatt
     3181 cgaactacaa aaatcagaat ccattttaag caactcaaat cgccttggta tttttgttga
     3241 tgataaacta gttgggactg tttcgcgtta ttgggtatgt aaacaaacaa gatggatgga
     3301 attgggaatt ggtatttatg ataaaaaatt ctggaacact ggtattggga aagttgctat
     3361 gttgcagtgg atagatagga cgtttcagga ttacttggag ttggagcatc tgggtttgac
     3421 aacttggtca gggaatcttg gtatgatgaa acttgctgaa aaattaagaa tgaaaaaaga
     3481 agctcatatt ccaaaagttc gttattatca aggtaaatat tttgatagta ttaaatatgg
     3541 tattttgaga gaagactggg agaaaataaa tgacggttat tatcaaatca atggaaactc
     3601 ctaaagagat agaaggtaaa tcccttgttc actggcaaat gtggagagag tcttatgatg
     3661 accttttgcc tgcggaattt caggatacaa tgacattaga aagatgtcga ttctttagtc
     3721 aaaagtatcc agaaaataca ttgattgcga tggatggcgt gaaggtggtt ggttttatca
     3781 gttatggcaa ttttcgtgat gagactattc aagctggtga aattattgct ttatatgttt
     3841 taaaagacta ttatggaaaa ggtatcgcac aaaagttaat gaaagcagct ttgactgctc
     3901 ttaatcattt ttctgaaatt ttcttatggg tattgaaaga taacaagcgc gccattgctt
     3961 tctatcaaaa aatgggtttt acttttgatg gacaagaaaa aatacttgaa cttggaaagc
     4021 ctataaagga aaaacgaatg gtgttctatt ctaaataatt ttaa
//
LOCUS       DAVKZB010000125         4014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKZB010000125 DAVKZB010000000
VERSION     DAVKZB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.125"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            24..476
                     /locus_tag="VXA80_001805"
     CDS             24..476
                     /locus_tag="VXA80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354252.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNTTIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1308..1667
                     /locus_tag="VXA80_001806"
     CDS             1308..1667
                     /locus_tag="VXA80_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354253.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1677..2006
                     /locus_tag="VXA80_001807"
     CDS             1677..2006
                     /locus_tag="VXA80_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV0354254.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            2029..3884
                     /locus_tag="VXA80_001808"
                     /pseudo
     CDS             2029..3884
                     /locus_tag="VXA80_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261786.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         1480 a          586 c          794 g         1154 t
ORIGIN      
        1 tttctaaaga aaatacacga tttttggagt ttaatattaa ctcgaaagaa tcttcgcaac
       61 aacttgatat tctctataaa gaagttggtg tattaagttt gggacaaaaa gttgtagcca
      121 tgcttgattt tttattagca tatagtgatt attctaaaga cttcagacca ttgattattg
      181 atcagcctga agacaatcta gacaatcgtt atatttacag gcatttagtt cagcagttta
      241 gagatgtgaa agctcaacgt caaattattt tagcaacaca taatactaca attgtaacaa
      301 attctatgac agatcaagtt gttattatgg agtcagatgg agttaacgga tggattgaat
      361 cacagggata tgttagtgaa aaatatataa aaaatcatat catcaatcaa ttagagggag
      421 gaaaagattc cttcaagcat aaaatgtcta tatatgagac ggctttatca gagtagagtc
      481 agaaaaagta ggttagaaat ttagcctact tttttctttg tccgacaggc atagtgtaca
      541 tctgaggtcc aagtcctctg tggatatttg ctgcagatga aaccaatagc gactcctaag
      601 cctgaatatc gtgaggtagg ggggatagga aggaattagc gaaatcaagg ttctacaaac
      661 agaatcgtga cttgaagcca tatatagcgg atgaggaact ctaaaatcca aataggtgtc
      721 gtaacctata tacgtaaatt acgagagtaa actaggaaag atgtacggct tattccgtga
      781 gcgtttagga cgtagtacaa cgaatcatgg gagtcagctg aacacatagt attgaagaaa
      841 tttctgtaat ggaaatggag cgaagaagtg aacaattaaa tgaatacctc tctaattaaa
      901 tttgtcaatt ctaattcctg gtatgaaaag acagtgacct gaaaatgtaa acgatgggag
      961 ctgatcataa atataggacg gtacatgcag tggtgttaga gattagtcct tacttgattt
     1021 gtgataactt ccccaaattt cttctgctat acttttctca acttttaaaa atccaactaa
     1081 gaattttacc tgggggtttg ggggcggagc cactaagtta tcttatcgtt agctgtcaaa
     1141 actggtaggt tttgataggc tggcgatatg atttttggga tattgtggac acaatatctg
     1201 agctcgcaaa gaccgaacaa ggagagactg aggtctttag gaatcgtact gacaatgtga
     1261 ggcagcctta cattgtcaga caagtataat gttgaaagaa tggtaagatg ggacaagaaa
     1321 ttaagttaat ccgtaagcaa tttagaatca cgagacaaga agaaaaacag ataaaagaaa
     1381 tgatgaggga acaaaaagtg gatagtttct cagaatttct tcgtcaaaat ttattgaaaa
     1441 agaattatca ggatagaatt tttgaaagtt ggttctccct ttggcagtct caaaagtttg
     1501 aacagattag tcgagatgtg tatgaagttc tggttgtcgc aagagaaaat catcaagtga
     1561 ctcaagagca cgtctcaatc ttattgactt gcgttcaaga attgattgca gaagtgaatc
     1621 aagtacagcc actcagtcgt gagtttcgtg aaaaatatat gggttaggag gatgtaatgg
     1681 tttatcgcta tcgtaccaat ctcaaaaaag tatttctaac tgatccagaa ttacaccaat
     1741 tgaatgaacg gattgctaag agcaattgtc aaaatttttc agtctatgcc agaaaagttt
     1801 tgcttaatcc caatatgtca tttgtcacga ttaacactga tacttatgac cagttagttt
     1861 ttgaattgag acgtattgga aataacatca atcaaattgc gcgtgcgatt aatcaaagcc
     1921 atctgatttc tcaggaccaa ttacaagaat tgagtaaagg agtcggagag ttaattaagg
     1981 aagtggacaa agaatttcaa gtgtaggtga aaagactgaa ggagtttcat ggtagttact
     2041 aaacactttg cgacgcacgg aaaaaaatat cgtaggcgtt tgattaaata tatcctcaat
     2101 ccagataaaa cagataatct aaaattagta tctgattttg gcatgagtaa ttacttagat
     2161 tttcctagcc atacagaaat ggtagaaatg tacaatgtta actttactaa taacgacaag
     2221 ttgtacgagt ccagaaatga ccgacaagaa aaacaccaac aaactattca tgcccatcat
     2281 ctcatccaat cattttctcc tgaagataat ctgacacctg aagaaattaa ccgcattggt
     2341 tatgagacca tgatggaatt gacaggaggt cgttttaagt ttatcgtagc aactcacaca
     2401 gacaaagatc atgttcataa tcacatccta atcaacgcta ttgaccgcaa ttcagataaa
     2461 aagttgatat ggaactatgc cttggaacga aacctccgta tgatttcaga ccgtatttct
     2521 aaaatggctg gtgcaaaaat tattgagaaa cgttattctt atcgaggtta taaaaaatat
     2581 agggaatcta gtcataagtt tgaattgaaa caacgtcttt attttttgat gcagcaatct
     2641 aaatagtttg atgatttttt agagaaagca aagcagttgc atgttcagat tgattttagt
     2701 caaaagcata gtcgattcat gatgacggat cgagcaatga caaaacctat tcgaggacgc
     2761 caactcagca aacgagatct atatgatgaa gaatttttta gaacgcattt tgctaagcaa
     2821 gagattgaaa gtcgtttaga atttttgttg aaccgtgtca attctttaga agagttaata
     2881 acaaaagcaa aagaattgaa tctaaccatt gacttaaaac aaaaaaatgt aacttttatc
     2941 ctgaaagaaa ataatcaaaa gataagtttg ggtcatcaaa aaataagtga taagaaatta
     3001 tatgatgtca aattttttca agattatttt aaaaataagg aagtcattgc ttcagaagga
     3061 ttagagaatt tacaggaaca gtaccatgct ttccaagaag aacgagataa ggataaagta
     3121 tccactgaag agattgagga agcctttaag acatttaaga agcctttaag acatttaagg
     3181 aaaaatgaga taccgttcgt gaatttgaag tggaacttgc agagaaccaa atagagaagc
     3241 tagttgataa gggcgtctat atcaaggtgt ctttcggtgt caaatagagc ggtcttattt
     3301 tcattcccaa ctatcaattg gatattatgg aagaagaaaa tcataaaaaa tataaagtct
     3361 acattcgaga aacgtcttct tactttgtct ataataaaga aaacatggat aataattgtt
     3421 tcatcaaggg taggacctta atcagacaat tgagcaacga tagccaaaaa ctcccctata
     3481 gacgaccaac tttaaaaagt cttcaagaga agatttctga aattaatctt atgattgaac
     3541 tatctaacac gaacaaacaa taccaagaaa tcaaagatga gcttgtatta gaaattgcag
     3601 agattgatat gaagctagaa gaaacccaag aaaaaatcgc caccttaaac aagatggcgg
     3661 aagtccttat caatttgaag agtgaagatg agatcggtag aaaattagcc aggtatgact
     3721 tttcaaaact gaacttaaca gaatcaactt cattagaaaa tgtgaacgaa gaaataagag
     3781 tattgcaaga aaacttagac tattatcttt atgagtttga gaaaagagca ataagattag
     3841 aaatttttgt aagcacgtta aatatggaaa agatgttttc gtaatggaca aattttagag
     3901 gagggataaa gctagaaatg ctggattatg cttgatttaa ggaactgcaa tacatggagc
     3961 acactcatat ggtttaagca caaataattt ttgacaatag gattaaaaaa ttgt
//
LOCUS       DAVKZB010000126         4010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKZB010000126 DAVKZB010000000
VERSION     DAVKZB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.126"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..196)
                     /locus_tag="VXA80_001809"
     CDS             complement(<1..196)
                     /locus_tag="VXA80_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEV0354255.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            478..1065
                     /locus_tag="VXA80_001810"
     CDS             478..1065
                     /locus_tag="VXA80_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV0354256.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            992..2635
                     /locus_tag="VXA80_001811"
     CDS             992..2635
                     /locus_tag="VXA80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0354257.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2646..4007
                     /locus_tag="VXA80_001812"
     CDS             2646..4007
                     /locus_tag="VXA80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV0354258.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDTIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
BASE COUNT         1320 a          814 c          637 g         1239 t
ORIGIN      
        1 acaaagagga cataattcct gaacctacac aaataagtgt tgctgctccc ccagttatcg
       61 gaccagtcgc agcagctaat agtactgctc caatacaacc accgattgca gatcctaaat
      121 tgcctcttcc tccactaact atttcgagtt cttcattatc cataacagaa aattgttcca
      181 tcatttttgt attcatgaca aatactcctt ttttcttttt ttatttttgt cttgttgtaa
      241 ctttgataag tttagtatat catcgttttt taaaattttt catccagatc ttgaattgtc
      301 atcgaaacgt cttgaattag cttttttatt tcaagccacc tctaaatgtt taaaaaaaat
      361 aatttctaat cactttttta ccattcagga agttttaatg actattcaag atttcataaa
      421 atatgaactt agttttatga cataatagac ctatccacta tatgaaagga attgccaatg
      481 acttcttata aacgtacatt tgttcctcaa atagatgcga gagactgtgg tgtcgctgcc
      541 ttagcctcga ttgctaaatt ctatggttca gatttttctc tagctcactt gagagaactt
      601 gcaaagacca ataaagaagg gacgactgct cttggcattg taaaagccgc tgatgaaatg
      661 ggctttgaaa caagacctgt tcaagcagat aaaacgctct ttgacatgag tgatgtcccc
      721 tatccattta tcgttcacgt taacaaagaa ggaaaactcc aacattacta tgttgtctat
      781 caaacaaaga aagactatct gattattggt gatcctgacc cttctgtaaa aatcactaaa
      841 atgtcaaaag aacgcttttt ctatgaatgg actggagtag ctatttttct agctaccaaa
      901 cccagctatc aaccccataa agataaaaag aatggtctac taagcaagct tccttcctct
      961 gattttcaaa caaaaatctc tcattgctta cattgttctc tcaagcttat tggtcactat
     1021 tatcaatata ggtggttctt actatctcca aggaatcttg gatgaataca ttccaaatca
     1081 gatgaaatca actttaggaa tcatctcagt tggtctggtt atcacctata tcctccaaca
     1141 agtcatgagc ttctccagag attatctcct aaccgttctg agtcagagat taagtattga
     1201 tgtgatttta tcctatattc gccatatttt tgaacttccc atgtctttct ttgcgacacg
     1261 tcgtacagga gaaatcattt cacgattcac agatgctaac tctattatag atgccttggc
     1321 ttctaccatt ctttctcttt ttctggatgt ttctattctg attcttgtag gaggcgtctt
     1381 actagcacaa aaccctaatc tcttccttct ttctcttatt tccattccta tatacatgtt
     1441 catcatcttt tcttttatga aacctttcga aaaaatgaac catgatgtca tgcaaagtaa
     1501 ttctatggtt agctctgcca ttatcgaaga tatcaacggg attgaaacta taaagtcgct
     1561 cacgagtgaa gaaaatcgct atcaaaatat agacagcgaa tttgtagatt atttggaaaa
     1621 atcctttaag ctcagtaaat attctatttt acaaacgagt ttaaagcagg gaacaaaatt
     1681 agttctgaat atccttatcc tatggtttgg cgctcaatta gtcatgtcaa gtaaaatttc
     1741 tatcggtcag ctgattacct ttaacacact tttttcttac tttacaactc ctatggaaaa
     1801 tattatcaac ctccaaacca aactccaatc tgcgaaggtc gctaataacc gtttgaacga
     1861 agtctatcta gtcgaatctg aatttcaagt tcaagaaaac cctgttcatt cacatttttt
     1921 gatgggcgat attgaatttg atgacctttc ttataagtat ggttttggac gagatacctt
     1981 aacagatatt aatctcacga ttaaacaagg agataaggtt agcctagttg gagttagtgg
     2041 ttctggtaaa acaactttag ccaaaatgat tgtcaatttc tttgaaccct acaaagggca
     2101 tatttccatc aatcatcagg atattaaaaa cattgataaa aaagtcttgc gccgtcatat
     2161 taattaccta ccccaacaag cctatatctt taatggctct atcttggaaa atttaacctt
     2221 gggcggtaat catatgatta gccaagaaga tattctaaga gcttgtgaat tagctgaaat
     2281 ccgtcaagac attgaaagaa tgcctatggg ctatcaaact cagctctctg atggagctgg
     2341 tctatcagga ggacagaagc aacgaatagc cctcgctcgt gctcttttaa ctaaatctcc
     2401 tgttttaata ctagacgaag ctactagcgg tcttgatgtc ttgactgaga aaaaagttat
     2461 agataatctt atgtccctaa ctgataaaac cattctcttt gtagcccatc gtctcagtat
     2521 agccgaacga actaaccgtg tcattgttct tgaccagggg aaaatcattg aagttggtag
     2581 tcaccaagag ttaatgcagg cgcaaggctt ctaccatcat ctgttcaata aataaggaga
     2641 atgtcatgaa tcctaatctt tttagaagcg tcgagtttta tcagagacgt taccataact
     2701 atgcgacagt attaatcata cctctttcat tattatttac tttcatcttg attttctccc
     2761 ttgttgccac aaaagaaatt actgttactt cccaagggga aatcacccct acaagtgtca
     2821 ttgcatctat tcagtcgacc agtgataatc ctatccttgc taatcattta gtggcaaatc
     2881 aagtagttga gaaaggtgac ttactcatca aatactctga aacaatggaa gagagtcaga
     2941 aaactgcctt agaaactcaa ttacaaagac ttgagaagca aaaagaagga cttggaatct
     3001 tgaaacaaag cttggaaaaa gcgactgatc ttttttctag cgaggatgag tttggctacc
     3061 ataatacttt tatgaatttt actaaacaat cccatgatat tgaactgggt atctcaaaga
     3121 ctaacactga agtttcaaat caagctaatc ttaccaatag cagttcatca gccatcgaac
     3181 aagaaattac aaaagttcaa caacaaattg gagaatatca agagctgaga gatactatcg
     3241 taaataaaag agctcgctta ccaactggca atccgcacca gtcaatttta aatcgttatc
     3301 ttatagcctc tcaagaacaa acacaaggaa cggcagagga gccattttta tctcaaatta
     3361 atcaaagtat tgcaggtctg gaatcatcta tcgcaagcct caaaattcag caagctggta
     3421 tcggaagtgt agcaacttat gataatagtt tagcaaccaa aattgaagta ctccgcactc
     3481 agtttttaca aacagcctca cagcaacaac taaccgtgga gaatcaatta acagaattaa
     3541 aagtacaact agatcaagca acgcagcgct taggaaacaa taccttaacc gctccaagta
     3601 aaggtatcgt tcatctgaac agcgaatttg aaggtaaaaa tagaattcca actggtacag
     3661 aaattgctca aatattccct gtcatcacag atacaagaga agtactaatc acttactacg
     3721 tatcttctga ctatctacct ctactagata aaggacaaac tgtaagatta aaactggaga
     3781 agattggaaa tcacggcacc accatcatcg gccaacttca gacaattgat caaactccta
     3841 ccagaacaga gcaaggaaat ctctttaaat taaccgctct tgcaaaacta tctaacgagg
     3901 atagtaaact catccaatat ggcttacaag gtcgcgtcac tagtgtaact acaaagaaaa
     3961 catattttga ttatttcaaa gataaaattt taacccattc tgattaattt
//
LOCUS       DAVKZB010000127         3971 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKZB010000127 DAVKZB010000000
VERSION     DAVKZB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.127"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..881)
                     /locus_tag="VXA80_001813"
     CDS             complement(<1..881)
                     /locus_tag="VXA80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0354259.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDATERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQK"
     gene            complement(999..1313)
                     /locus_tag="VXA80_001814"
     CDS             complement(999..1313)
                     /locus_tag="VXA80_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV0354260.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(1443..1682)
                     /locus_tag="VXA80_001815"
     CDS             complement(1443..1682)
                     /locus_tag="VXA80_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV0354261.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(1734..3143)
                     /locus_tag="VXA80_001816"
     CDS             complement(1734..3143)
                     /locus_tag="VXA80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0354262.1"
                     /translation="MGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVAREITSGVLE
                     GKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEPNEEGLQFYD
                     NLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVVFKRYKDKVK
                     YWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVASARAVKIGHE
                     INPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYPEHIFKYWER
                     KGISVDFAAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETEDLVKNNYVK
                     ASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVTADGMVHDDYRIEYLDA
                     HIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDNGKGSYNRSP
                     KKSFGWYKEVISSNGESVE"
     gene            complement(3359..3697)
                     /locus_tag="VXA80_001817"
     CDS             complement(3359..3697)
                     /locus_tag="VXA80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV0354263.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            complement(3853..>3971)
                     /locus_tag="VXA80_001818"
     CDS             complement(3853..>3971)
                     /locus_tag="VXA80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0354264.1"
                     /translation="LELLCEEFGHKLLPLPPYSPEYNSIEKTWALLSCSCFN"
BASE COUNT         1312 a          800 c          504 g         1355 t
ORIGIN      
        1 tttttttgac attgttgact cttagctaac aaatgcaaag taatgtagtc tatttcctga
       61 actggaaatt cctgatttgt tacttctctt actttagaaa gaattctttg ggcaaccttt
      121 ctctctgttg catcatcagt catctgacag tctatatttt ttatttcaaa tccggatttt
      181 aaacgaatca cagacaatgc tatgtgaact actaaattct gtagtacaaa atcagatagt
      241 tttaggttgg cctcttggca ttcatccaaa acaattctag caaattcttc taatggaaca
      301 gtttgatcaa aaaagttaaa ttttacatag caatgtattg ttttaaaaaa ttgattctct
      361 aggaaataat ttatgataaa acgtcgttta tcacgttcct cgcctgagac ataaactcct
      421 ttattcgccc tactctcaat ggacaaatta tactctgata acatcactcg tatctttctg
      481 aaatcatgag ataatgttga acgactaacg taaagttcat cagctaaatc atcaaaaaga
      541 actggaactt gctcaaataa taatttattt aagataaata ctaaacgatc atcacctttt
      601 gaaaccgcag ttttcgtata gtcttcttcc agttcataag tttgtctaaa ctcctggtaa
      661 gcgccttgat tctcaaaaaa tatttgatac ccttgacctt gttttgaaat caaccggact
      721 ccttgaataa tcattgtctt ctcaattaat ttcagtacat tacggacagt tctatctgaa
      781 caggataaat attctgctag ttctttgctt gtaacaaaac gttccttatt ttttattaaa
      841 aattgaagga tatctttctc tttaatgttt aacacattca ttccctccta aaacgtatgt
      901 tttcatatat tgaagcatat tatacactta aatcaattta tatcaaactc aaaacaattt
      961 atcttaactt aaatatttat tgacatttca tgtgttcatc aaatattctc aagaatcaaa
     1021 ttagccattt tttcaattcc cattggaata ggaatatagg cttgaggagg tatttgtaca
     1081 actggttttc ctgctttaga accagcctct tcaaattgct taaagtacat ttttgtttga
     1141 gggctgacaa gatacaaatc aaaagctgct gctgcgatag ctttccctcc ttcagtagca
     1201 ctaatagcat caactacaat atctttccct tttcctttta gaaactctgt tgttttctgt
     1261 gccataagtg atgaagacat tcctgctgca caaataatta aagcttttgc cataatattt
     1321 tctccttttc ttaaatccaa tcaaagctgt gctaagttgg cttatttgtt atctattttt
     1381 attataaaat aaagcgtttc caatgacaat tccctcattt tcctaaatga tatggaaaaa
     1441 aattatttat acttcaattt ataaaataaa attattcctg agagtagaaa tgaaacacta
     1501 tttgctaaaa tcaaaggcaa gtctcctata cgaataccat gagcaagcca caatgcaata
     1561 ccaataactt gcataacata catacctaga gcaatagatc ctgtgtcctt tgtcttaact
     1621 acacgaaaaa cttgtggtaa aaatgcaaat gttgttaaaa ttgctgcaat acttccaatc
     1681 atatgtcacc tcaatatgct aaacaaactg agaataatct cagtttgttt atactattct
     1741 actgattcac cgttagatga aataacttcc ttataccagc caaaagattt tttcggggaa
     1801 cgattataac ttcccttccc attatcatct ttatctacat aaataaagcc ataacgtttc
     1861 cgcatttcac cggtaccagc tgaaaccaaa tcaatacatc cccatggagt ataacccatt
     1921 aaatcaacac catcttcaac tacagccttt ttcatttcac gaatatgggc atctagatat
     1981 tcaattctat aatcatcatg taccatacca tctgctgtaa cttgatctat agctccaaaa
     2041 ccattttcaa caataaagag tggtaagtga tagtggtctg taaaccaatt taacgcataa
     2101 cgcaaacctt ctggatcaat ttgccactcc cattcagaag ccttaacata attatttttc
     2161 actaaatctt ctgtttcaag ataatcaaaa taaggattat tttcacgatg agagtcgata
     2221 gcaaaggaca tatagtaact gaaaccaatg taatctacag tcccaccaag taaatcttct
     2281 ttatcctggg cagcaaaatc aactgaaata ccttttcgtt cccaatactt gaaaatatgc
     2341 tcaggatatt tacctaaaac atgcacatca gcaaaataat aacgcttctg catagctttc
     2401 attgccatta agatatcctt aggattgcaa gtagctggat aaattggaca catcgcaatc
     2461 atacaaccta tttgaaaatc tggattaatc tcatgaccaa tttttacagc tcgtgcagaa
     2521 gcaactaatt cgtaatgtgc tgcttgatac ataattgctt ctctattatc accttcctca
     2581 tatacaatac ctgagttagt aaatggtgca aaatcttcct gataattcgc ttgattattg
     2641 atttcattga aagtcatcca atatttaacc ttatctttgt aacgtttaaa tacgacttct
     2701 gcaaaacgag caaagaaatc aatcaatttc ctatttttcc aaccaccata ttcggtcact
     2761 aagtgataag gcatttcaaa atgagataga gtgatgacag gttcaatacc attctttaag
     2821 cattcatcaa aaagattatc ataaaactgt aatccttctt cattcggctc taactcatca
     2881 ccttttggaa agatacgcgt ccatgcaata gaggtacgga agcacttgaa tcccatttca
     2941 gcaaaaagtg ctatgtcttc tttataacga tgataaaaat ctatcgcctc atgatttgga
     3001 taatatttac cctctaaaac tcccgaagta atttcacgag ctactccatg acgaccagca
     3061 gtcataacat cagcaacact aattcccttg ccaccttctt gccatccacc ttcaagttga
     3121 tgagcagcaa cagcaccacc cataaaaatc catctttaaa agtagtcatc ttttttcctc
     3181 ctgactttga tactcttatt ataaacctca aaccaaaaga tgaaaacgca ttctttttcc
     3241 ttattgttaa gaaaagaagt aatttttaat ggaaatagaa caatatcttc ttgtattctc
     3301 gtaatgatat ctttacgatt ttcaatactt tcaaactaca aaaactctca caataattct
     3361 aattccctgt gtctataaac gacttatcgc tttctggcat cccagaatca tcttctatat
     3421 aacgttcaac ttgcatctgc aagtgatatt tttttcttaa atctaagatt ttctgcattg
     3481 tctttgattg atgatgttca tctaaagctt cttgatttat ccactgatca ataaggagaa
     3541 tagttccctc tttttcaatt ggtaaaaaat attcgtattt caagttacct ttttgatttc
     3601 taatttcttc aacaaggcca ctatcaagca tttctcttgc aaactttatt gcactatctc
     3661 catcaccttt ataatataca tgaatagtca atgtcatctt atatccttca aaatcatcct
     3721 tcaattttaa aaaaacaagt ttagatgagg atctaaactt gttttttatg aactaattat
     3781 ctaacgtttc gccattactt tcaatcactt ctttatacca ataaaatgat tttttcttat
     3841 agcgatttat agtcaattga aacaagagca ggacaaaaga gcccatgttt tctcaataga
     3901 attgtactca ggtgagtagg gaggaagagg taaaagttta tgcccaaact cttcacacaa
     3961 gagttctaac t
//
LOCUS       DAVKZB010000128         3952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKZB010000128 DAVKZB010000000
VERSION     DAVKZB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.128"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            182..466
                     /locus_tag="VXA80_001819"
     CDS             182..466
                     /locus_tag="VXA80_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354265.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            459..707
                     /locus_tag="VXA80_001820"
     CDS             459..707
                     /locus_tag="VXA80_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354266.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            803..1021
                     /locus_tag="VXA80_001821"
     CDS             803..1021
                     /locus_tag="VXA80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354267.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(1389..3713)
                     /gene="pflB"
                     /locus_tag="VXA80_001822"
     CDS             complement(1389..3713)
                     /gene="pflB"
                     /locus_tag="VXA80_001822"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV0354268.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1222 a          719 c          756 g         1255 t
ORIGIN      
        1 aaagattcat tttacatttc taaacattct tttctaagat gaaaaacaga atttttcgat
       61 tgtgatttaa agcaacaaga agattttcag tatcatccta tagatacgag ctaattaaga
      121 aaaactacat tttcgaatat aaactacaat aatataaact aaattttata ggaggaagac
      181 aatggattgg tacgattata tgatacaggc atccaaacaa tcacaattca acgcaagcca
      241 ttggtttcgc tatttgcgaa aagttatttt tgaagactat tcttatttaa caaaccaaga
      301 tgtagaaaag ttgctagact ccaaagaact aacccgtttt caaaaaatta gcttgaagta
      361 tgcctttcaa gagcatactc caactcataa atatgtgatt tcattaaata aacctgctaa
      421 gttaaccaat gttcaaaaat tgatggagaa atacaaacat ggataaaatg aaaccggtct
      481 tccaagccct aaataaggaa ttaattcagg aaaatctgac tttaacaatt atctgtgtcg
      541 atggttatgt cttagaatat catggtttac gtgccacaca agatgttgat gctttttatg
      601 accaaaatca gaaaatcaat gaaattattg ctcgtgtagg aaaacagttt aatcttaaca
      661 tccacgaaga gttatggctc aacaatcatg ttgcaaagca aatatgaaca aacaacctcc
      721 cttaagttta tgcgaatcct tgtactcatt tgagaatctg accgttttag tagtccctat
      781 tgagtatgtg ttaggaatga agatgatgag tatcagagag caggatttgc aagatattgg
      841 tgcaattatt aaatacaaaa actttcattc accattcgat acctttaaat atttaaaaga
      901 catgggattt gatactattg acctctctgt tctattagaa ggttttagtt atgcttatgg
      961 tatggattgg ttagaaaaat tttttaaaga aaatcaagat aaacttcgcg aattttatta
     1021 acagtatcaa acaagatgat tgtcaattag ccagtcgtct tgttttttat gatgaaatga
     1081 aataaaatct gaacaaatcg cttagagaat tccagtcaat ttctagcgct gtttgagacg
     1141 tttcagtgga ctatagtaaa atgaaataag aataggacaa atcgatcagg acagtcaaat
     1201 cgatttctaa gaatgtttta gaaataaagg tgtactattc tagtttcaat ctattatatt
     1261 tcagattcgg tccactattc cttcccaaaa acattgcata ttgcgccgaa ggaattgctc
     1321 atactatccc aagcacaaaa ccctcagtcg tttgaccgag ggttcctttt cattattcaa
     1381 aaacttgatt agctcaatgc atccaaggca tcatccattg aaagaacttc gtggaagaca
     1441 cgttgtgtca attcagtttt ttgttctgga gtgaggtatt tagtatttac acagtatcca
     1501 gagatacgta cgataacgtc ttcaccggac atgatttttt cgtaaacatc gttcaagtcc
     1561 ataacgttca agttaacgtg ttgtccaccg ttttcgaagt aaccatcaag gattgttacc
     1621 aagttatcaa cttgttcatc acgagtctta ccaagagcgc gaggtgatac ttgtgtagtc
     1681 aatgagatac cgtcagctgc ataactaaag tcaaggctag aaagtgagtt caagttttgc
     1741 aaccaaccac ctttagcttt gttagatggg ttagcacctg gtgagaagaa ttcaagttta
     1801 gacaagttca cagaaccatc ttcgttgagg tatacacctt tgtgaactgg tgagttacca
     1861 gtttgtttag agtaagcaac gttagatgtg attgtcaaaa gtgatactgt agcttctgcg
     1921 tctttgtata gtttgtggct acgtagacga gttgtgtaag cttcgatcaa ccattctgcc
     1981 aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt agtcaccgat tgtttcgtaa
     2041 tcgtagatgt agccatcttc gtcacggatt ggtttaactg tagcgtattt gatagctgac
     2101 aatgtatcaa cagtgttagc aaatccacag ataccgaatc ccatgttggc acgttgttta
     2161 gttggcaaga aggccatttg aacagcttcg tagttgtacc tatcagtcat gtagtggatg
     2221 atgttcaagg catctacgta agtgtcagtc aaccagtcaa gagatttttc aaagttcgct
     2281 ttaactgatt cgaattcaag aacttcgtca cggattggtt cgatatcaaa tactttgtag
     2341 tctttgtgaa catcgtcgta accaccattc aaaccagtaa gaagggcttt aagaacgttt
     2401 acacgagcac cgaagtactg gatgttgtgg cgttgttctt cattttctgg atcaagtgga
     2461 gacacacagc atgagataca gctcatttca ccatatccgt ctttagccat tgttgttaca
     2521 ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca tacagtagcg acggaagttg
     2581 tatggcaatt tgtcagtcca aagaactgtc aagtttggtt ctggtgagtt accgatgttg
     2641 tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac ggtgacgacc gtcgttaccc
     2701 ataccagcca tagaagttgt gataaaggtt gggtcacctg agtacaattg gtcataagct
     2761 tttgtacgag caaatttaac tgtacgaagt ttcataacga aatcatcaac gaattcttgg
     2821 atttctgatt cagtaaatgt accacgagca aggtcacgtt ctgcaaagat gtccaatacg
     2881 attggtacac gacctagaga tgtagcagca ccgttaatca cacggcagac agccatgaaa
     2941 gcaatgttaa cccattggat tgcttctttc acgttcatcg ctggtttgcg aacatcaacc
     3001 ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt ggtattgaag gtttacttct
     3061 tcacgaagac ggattgtttc ttcatcgatt tctttgattg cattccagtc gtttactttt
     3121 tcttgcatca agtagtctgc accgtaaaga gcaagacgtg cgtaaacacc gatgatacgt
     3181 ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg catgacgagc gcgacgaata
     3241 tttgaagtgt aggcacggaa aataccgtcg ttaactgttg ttacatattt agtgaagatt
     3301 tcgtgaacag ctgggtctgg ttcgtatcca ttttctttta aagtagtttc agccatacgg
     3361 ataccacctt ttggcatgaa gttcaatttg aagagttcat cattttggat accaaagata
     3421 acttcatttt ctttgtcgat aaatccagca gggatatcag cgatagatgt tggacgagtg
     3481 tccattggga aacgagtttc ttcgtagtgt gctttagttt cttctacaat tttcttgatg
     3541 tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt ctccatcata aggtgtgtag
     3601 ttagcttgta caaatcgtga tacacttgct ttttctttcc aatctacgcc tttgaagcct
     3661 tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg tcttaacaac catattaatg
     3721 tcctcttttt tctagtaaca gtcatctgtt acattcataa gacaagtata ccatatagta
     3781 accgatttca acaagtgaaa attccctatt tttacacttt cttttctaaa acagtctata
     3841 ttttatttca aactgtatta tatttttgaa aaaaataaag tccttttttc tttttttcag
     3901 aaaaaagggt ataataaaag aaaataagca gtaacactca atggaaatcg aa
//
LOCUS       DAVKZB010000129         3846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKZB010000129 DAVKZB010000000
VERSION     DAVKZB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.129"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..156
                     /locus_tag="VXA80_001823"
     CDS             <1..156
                     /locus_tag="VXA80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0354269.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSRA"
     gene            complement(417..1391)
                     /gene="pta"
                     /locus_tag="VXA80_001824"
     CDS             complement(417..1391)
                     /gene="pta"
                     /locus_tag="VXA80_001824"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV0354270.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1435..2331)
                     /locus_tag="VXA80_001825"
     CDS             complement(1435..2331)
                     /locus_tag="VXA80_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0354271.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2328..3146)
                     /locus_tag="VXA80_001826"
     CDS             complement(2328..3146)
                     /locus_tag="VXA80_001826"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV0354272.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3130..3801)
                     /locus_tag="VXA80_001827"
     CDS             complement(3130..3801)
                     /locus_tag="VXA80_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV0354273.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
BASE COUNT         1149 a          851 c          704 g         1142 t
ORIGIN      
        1 tattcaaacg ccaaattgga agcgaccaat aatctcatca aacttatcaa acgcaatgcc
       61 tttggttttc gaaactttga aaacttcaaa aaacggattt ttatcgctct gaatatcaaa
      121 aaagaaagga caaaatttgt cctttctcga gcttagctga cttcaaccca ctacagttga
      181 caaagagcca aaaaattctc ttttcatact catttaaaat caaaaaggga atttatcgtt
      241 ttacggacat gtatattaca gatttctata ggtcgtgtat agacaggata ttatcgcttg
      301 gaagttttcc tttttgacac caatcagtaa tttagttctc aggaattaat ggcagtgctt
      361 ttcgtacata gtttcataac tacagtatag catagtatat cgctttatag ttttcactat
      421 tgatgaactg cttgagctgc tgtgatgagg gtcaacttgt aaacatcatc tgcattacat
      481 ccacgagaaa gatcattaac tggcttgttt aaaccttgca aaacaggtcc tacagccgca
      541 aagccaccca ggcgttcagc catcttgtaa ccgatatttc ctgcctcgat acctgggaag
      601 atgaagacat ttgcttgacc agctaccgta cttccaggag ctttcagagc tgcagtttca
      661 ggaacaaagg ctgcatcaaa ttgcaactca ccatcgattt caaggtcagg acgcaagtcg
      721 tgagcaattt tagttgcttc aacgacctta tcaacgcttt caccaaaccc tgaaccttta
      781 gtagaatagc tcaacatggc aattttaggt tcgatgccaa acatcttagc tgtgattgct
      841 gagttgatgg caatttcagc caaggcttct gcatctggat tgatgttaat ggcacagtct
      901 ccaaataggt aacgttccgt accacgaacc atgaggaagg ctcctgaagt acgagttaca
      961 tttggacgag ttttgatgat ttgtagagct ggacgaactg ttgaagctgt tgagtgaatc
     1021 gctcctgaca ccattccatc aaccaagccc aagtaaacca acatcacacc aaagtagttg
     1081 acatcttcaa ccaaaacctt gcgtacatct tcttcagtca ttttgccctt gcgacgctcc
     1141 accaaggcag aaaccatttc ttcaaattga ggataatgtt gagggtcgat gacctcataa
     1201 ccatccatga ttccttcaat ttcaagataa attttaattt tttcaggatt tccaagcaaa
     1261 acaggaatca cttctgtttc ttttactaag cgttttgttg cttgaagaat acgaggctct
     1321 tccccttcag ggagaacgat acgagcattt ttaccaacaa ggttggcttt gagactttca
     1381 aaaacttcca tgagttttct cctttaagat aataattata ctctgagaca gtttttatag
     1441 agtattagtt gataactggg taataaggtt actgaaatca tccggcaagg gactttctaa
     1501 ctgcaagtct tgctctaaaa atggatgata aaaggatagg taatggcaat gcagagcctg
     1561 acgttgaata ccatcttcca gactaccacc atacaaatca tctcccagca aaggaaaacc
     1621 gatatgagaa aaatggactc ggatttgatg ggttcgacca gtgtgcaggt gaatatagac
     1681 caagtgaata tttccataag aagctacaat cttgtatgaa gtatgggcat actttccgcc
     1741 tttagccact cgtctggtaa taatggaatc ttcatcacgc gcaatcggag caataatttc
     1801 cccttctggc tccaaatgtc catctccctt aaccaaagca aagtagcgtt tctcgataga
     1861 tttcttctgc aactgcttgt ctaatcgtgc atgggcataa ccgtgcttgg caaagagcat
     1921 caagccagaa gtgtccctat ctagtctggt aacaatgtga acctgctgat tttcataatt
     1981 ttgcttgaca tagtaaccct tgataaaatt ggcaatggta ttagagtgat tgacactagg
     2041 aatagaagcc actccatagg gtttattcaa gactagaaag tggtcatcct catagagaat
     2101 atctaatgga tgctcaatag cctccaaggt ttcaaagcct tcctcagcgg gaatgtaaat
     2161 ggtaacgtag tctccaacgt ccaatagata cgttgcattt tgcggttgat tattgaccag
     2221 aatagctcca cctcgaaact taatcttggc cagcaatccc ttagaaacct cgtgcttttt
     2281 taaaaaggtc ttaaccttga catgttcatc tgcgataaat tcaaacctca ttcatccacc
     2341 tcaccgataa aggcatcctt aacacggttc cagaaactgg tatggctagg agtcgcgaca
     2401 aagtgaatct tatgatggtc gatttgatac tcaatacgct caatattacg gaaagaataa
     2461 acgctattgt caaccgaaat agtatgataa tcgtttcttg ttggaataag ttcaatctta
     2521 tccttcttag gcacaataat ggaagagccc agtgttcgat aaacacgatt attaaggctg
     2581 gcaatttccg ttaattgcaa agcttcaatg gtagggtgta aaacagcacc gccaagagac
     2641 ttgttatagg cagtactacc agtcggtgtc gaaactgtta gcccgtctcc acgaaaacgt
     2701 tcaaagggaa caccatttat tacaatatct gccaccatgg ttcgatcaga cctgcggatg
     2761 ctggcttcgt tgagtgctct gaaaatctta acttcaccat tttcaagaaa gaccttcaca
     2821 ttcagaacag ggtaagaaac ccttgcccca gtatctagct gcaaattagt cactagcttg
     2881 tccaactcaa aatcacgata atctgtatag aagcccaaat gtccagtatg aagaccgata
     2941 aagcggacct tgtcaagctg attttcgtac ttatgaaagg ccgacaagag cataccatcc
     3001 ccgccaatgg aaatgacaat atccggattg gtatcattga gtataaactg atttctcttc
     3061 aaacgatctc gcaattcata caaaaccctt tgactctgcg gttttctatt ggctatcaga
     3121 tcaattcgtt tacctgtatt cttcatctgt atcgtcactg tttcctacac cgtcatttaa
     3181 ttttctactc aaaggatcaa aaagtgcctg ggcttcttgg atatcatcac gaatttcacc
     3241 catttcttca tccaactgat gggcgattct agctgtaatt tccagtcgct tcttaatctc
     3301 atctgggaaa tccccttggt acttgtagtt gagagaatgt tctatcgttg cccagaaatt
     3361 catggccaaa gtacgaattt gaatttctgc caaaatagtc ttagctccat tgatggtatc
     3421 aaccgtatat tctactacca catgatagga acgatagcct gatgcttttc tatgagtaat
     3481 gtaatctcgc tcctgtatga ttcgcatatc ctgacgcttg tgcaaaatat ccactacttc
     3541 cttgacgtca tctacaaact gaaccatcac acgtaagcca gcaatatcct gcaaatcgtg
     3601 ttccaaggtc gcataagtaa tgccacgacg agccattttt tctttgatgc tctcaattgg
     3661 cttgactcga ccggtcacaa actcaattgg agaatgctta ttttgcttac gatattgctt
     3721 acgaatacca cgaagtttaa tctttaactc accaacagct tgaatgtaag gatctagaaa
     3781 ttcttcccat tctaaggtca tatattctcc cttgttctca tattatccgt caaaaatatc
     3841 tttgat
//
LOCUS       DAVKZB010000130         3764 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKZB010000130 DAVKZB010000000
VERSION     DAVKZB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.130"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            93..311
                     /gene="nrdH"
                     /locus_tag="VXA80_001828"
     CDS             93..311
                     /gene="nrdH"
                     /locus_tag="VXA80_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV0354274.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            392..2551
                     /gene="nrdE"
                     /locus_tag="VXA80_001829"
     CDS             392..2551
                     /gene="nrdE"
                     /locus_tag="VXA80_001829"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV0354275.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGECYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2739..3701
                     /gene="nrdF"
                     /locus_tag="VXA80_001830"
     CDS             2739..3701
                     /gene="nrdF"
                     /locus_tag="VXA80_001830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV0354276.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1173 a          811 c          700 g         1080 t
ORIGIN      
        1 aaagaacacc actatattta gttcaaatca ttgacaaaat tttattttct gatatactaa
       61 gatagtatta aattttgaag aggagttaca caatggtaac cgtttattct aaaaacaatt
      121 gtgtccaatg taaaatgacc aagcgtttct tggacagtaa taatgtctct tatcgtgaaa
      181 tcaatcttga tgagcaacct gagtacgtcg atcaagttaa agagctcggt tttagcgcag
      241 ctcctgttat ccaaacacca actgaagtct tttcaggttt ccaaccagga aaactgaaac
      301 aattagcata atcttagtac atcatccaga agaaactact tttagggcta acttagaagc
      361 ctttcttttg taattagata agggaaattt tatgggatta aaacatcttg aagacgtgac
      421 ttacttccgt cttaataacg aaattaaccg tcctgttaat ggacaaatca tgcttcataa
      481 agataaggaa gccttggatg ctttctttaa agaaaatgta gttccaaaca ctatggtttt
      541 tgattcaatc aaagacaaaa tcaattacct cattgaacac aactacatcg aaacagcctt
      601 tatcaagaaa taccgtccag agttcttgga agaattggct caatttatca aagaccaaaa
      661 cttccaattc aagtcattca tggcagctta taaattttac aatcaatatg ccttaaagac
      721 taatgacggt gaatgctatc ttgaaaatat ggaagaccgt gtcttcttca acgcccttta
      781 tttcgctgat gggaatgaag ctgttgcaat cgatattgcc aatgaaatca tccaccaacg
      841 ctaccaacct gctactcctt ccttcttgaa tgctggacgt gctcgtcgtg gggagttggt
      901 atcttgtttc ctaatccaag tgacggatga tatgaactct atcgggcgtt ctatcaactc
      961 agctcttcaa ctttcacgta ttggtggtgg tgtgggaatt accctcagca accttcgtga
     1021 agctggtgca cctatcaaag gctatgaagg agctgcttca ggtgtcgttc ctgttatgaa
     1081 gctttttgaa gacagcttct cttactccaa ccaattgggg caacgtcaag gtgctggtgt
     1141 tgtctacctc aacgtctttc acccagatat catcgctttc ctttcaacta agaaagaaaa
     1201 cgctgatgaa aaagtacgtg tcaagactct atcacttggt gttgtagtac ctgataaatt
     1261 ctacgaattg gctcgtaaaa atgaagaaat gtacctcttc agcccttact ctgtagaaaa
     1321 agaatacggt gtgccattca attacatcga cattactgaa aaatacgatg aattggtcgc
     1381 aaatccaaat attcgcaaga caaaaatcaa ggcgcgggat ttggaaactg aaatctctaa
     1441 attgcaacaa gagtctggct atccttatgt agtcaacatc gatacggcta accgtgcaaa
     1501 tcctgttgat ggaaagatta tcatgagtaa cttgtgttct gagattcttc aagttcaaga
     1561 accaagcttg atcaacgatg ctcaagaatt ccttcaaatg ggaacagatg tttcatgtaa
     1621 cctgggttca accaacgtgg ttaacatgat gacttcacct gattttggtc gttctatccg
     1681 tgctatggtt cgtgccctta ctttcgttac agatagttca catatcgtag ctgtaccaac
     1741 gattgaccat ggaaatagcc aagcccacac ctttggtctc ggtgccatgg gactgcacag
     1801 ctaccttgcc caacaactca ttgaatatgg ttcacctgag tctgttgaat ttacaagcat
     1861 ctactttatg cttatgaact actggacctt ggtagagtca aacaatattg cacgtgaacg
     1921 tggtattacc ttccacaact ttgaaaaatc agactatgct aacggaagtt actttgacaa
     1981 gtatgtaact ggtgaatttg ttccaacatc agaccgtgtt aaagaactct tcaaaaatgt
     2041 ctttatccct ggtgttgctg attgggctga acttcgcgac aaggttcaag aagatggtct
     2101 ttaccaccaa aatcgccttg ctgtagcgcc aaatggttct atcagctata tcaacgacgt
     2161 ttctgcttct atccacccga ttacgcaacg tatcgaagaa cgccaagaga agaaaattgg
     2221 taaaatctac taccctgctg ctggcttgtc tacagaaacc attccttact acacttctgc
     2281 ctacgatatg gatatgcgta aagtcatcga tgtttacgct gctgcgactg aacacgtgga
     2341 tcaaggactt tcactcaccc tcttcatgcg cagtgacatt ccaaaaggcc tttacgaatg
     2401 gaagagggaa aataaacaaa cgacacgtga cttgtccatc cttcgtaact atgcctttaa
     2461 caagggaatc aagtctatct actacgtccg tacctttaca gatgacggtg gagaagtcgg
     2521 cgccaaccaa tgtgaaagct gtgtgattta gtttctagca tagaaacact atttttccct
     2581 aagcagcact gataaaatca aatctactat gtcataacat aatttatgta acattttaaa
     2641 agtcgggctt ccgacttttt gtgcgatact cctatagaat tatggtaaag tagaaataat
     2701 tgtgagttcg caatactaaa cacagaaaga gattttaaat ggaaacttac tacaaagcca
     2761 ttaactggaa tgccatcgaa gatgtcatcg acaaatcaac ttgggaaaaa ctgacggagc
     2821 aattttggct cgatacacgt attcccttat caaacgactt ggatgactgg agaaagctat
     2881 ctaacaaaga gaaagacttg gtcggaaaag tttttggtgg tttaaccctt cttgacacta
     2941 tgcaatctga aactggggtt caagcccttc gcgcagacat ccgtacacca catgaggaag
     3001 ctgtcttcaa taacatccaa tttatggaat ctgtccacgc taaatcttac tcatcaatct
     3061 tttctacctt gaatactaag gctgagattg aagaaatttt cgaatggacc aataccaatc
     3121 cttacctaca aaagaaggct gagattgtca acgaaatcta cctaaacggc agcccacttg
     3181 aaaagaaagt tgccagcgtc ttcctcgaaa ccttcctctt ctactctggt ttcttcactc
     3241 ccctctacta tctcggtaac aacaaacttg ccaacgttgc agaaatcatt aaattgatta
     3301 ttcgtgacga gtctgttcac ggaacctaca ttggttacaa attccaactt ggtttcaatg
     3361 aattacctga agaagagcaa gaaaaactca aagaatggat gtacgaccta ctctatactc
     3421 tttatgaaaa tgaagaaggc tacacagaga gtctttatga cggtgttggt tggactgaag
     3481 aggttaaaac cttccttcgc tacaatgcca ataaagctct catgaacatg ggacaagatc
     3541 cacttttccc agattcagct gaagatgtca acccaatcgt tatgaacggt atttcaacag
     3601 gaacttccaa ccatgacttc ttctctcaag tcggaaatgg ttaccttctt ggcgaagttg
     3661 aagccatgca agatgatgac tacaactacg gtttggacta atactcttcg aaaatcaaat
     3721 tcaaaccacg tcagcttcgc cttgccgtac tcaagtacag cctg
//
LOCUS       DAVKZB010000131         3754 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKZB010000131 DAVKZB010000000
VERSION     DAVKZB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.131"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            14..853
                     /locus_tag="VXA80_001831"
                     /pseudo
     CDS             14..853
                     /locus_tag="VXA80_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            846..1559
                     /locus_tag="VXA80_001832"
     CDS             846..1559
                     /locus_tag="VXA80_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0354277.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1881..1973)
                     /locus_tag="VXA80_001833"
                     /pseudo
     CDS             complement(1881..1973)
                     /locus_tag="VXA80_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2294..2995
                     /gene="pyrF"
                     /locus_tag="VXA80_001834"
     CDS             2294..2995
                     /gene="pyrF"
                     /locus_tag="VXA80_001834"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV0354278.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            3029..3661
                     /locus_tag="VXA80_001835"
     CDS             3029..3661
                     /locus_tag="VXA80_001835"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV0354279.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADADVKLVTLSNYSELIHLAQEEGYITPEGLNLLKRFKEDQENWQEG"
BASE COUNT         1182 a          646 c          764 g         1162 t
ORIGIN      
        1 taaaggaggt gcaatgagta tgattgaagt tagccattta tcaaaaagtt ttggtgataa
       61 aatagcttta aataatataa gcttcactgt taaagaaggt tagatttttg gatttttagg
      121 accatctggt tctggaaaga ccacaacgat taatattctg actgggcagt tccttgccga
      181 taaaggacaa tctattattt tgggacaaaa atctcaaaat ttaacaagcg gtgaattaaa
      241 gagaattgga ttggttagcg atacaagtgg attttatgag aaaatgtctc tgtataacaa
      301 tcttcttttt tatagtaaat tttataatat tagtaaatca cgtgttgata atttgttaaa
      361 gcgagtagga ttatatgata gttgcaagat ggtagcagga aaattatcca ctggaatgag
      421 gcaacgaatg cttttagcac gagctcttat caacaaaccc gctgtactct ttctggatga
      481 accgacctca ggtctagatc ccacaacttc tcgaacaatt catgagttaa ttttagaatt
      541 gaaaacagca gggacaacga tttttctaac gactcatgat atgaatgaag caactctttt
      601 atgtgattat gttgccttat taaataaagg gaaattagtt gagcaaggag ctccttctga
      661 actcattcaa agatataata aagataaaaa gattaaggtt acagattata atgggaatca
      721 gataactttt gattttacat cactagaaca ggtatctcag gctgatctgg aaaatatttt
      781 ttcaattcat tcatgtgaac ctactttaga agatattttt atcacattaa caggaggaaa
      841 gctaaatgct taaacggttt ctggctttgg tatggttgcg ttgtcaaatt atcctttcca
      901 ataagagtat tttattgcaa gttttagtgc cttttgcttt cacatatttt tataaatatc
      961 ttatggaaac acaggggaag gtcaacgatc aacaggcatt agttcttttg atgatgtgtt
     1021 tacctttttc tttggctttg gctgttggaa gtcctataac tattatcttg tctgaagaga
     1081 aagaaaagta caatttacaa actcttctgt tgagtggtgt taaaggatcc gaatacattt
     1141 tatcaacgat gtttcttcct tttttgctaa cttttgtgat tatgggaact actcctctta
     1201 ttttaggagt tacaattgta catactttta attatattac aatcgttctt ctaacctctt
     1261 tatccatcat tttattctat ttattgatag gtttaaccgc gaagagccaa gtagtagctc
     1321 aggttatcag tcttcctgct atgattttag ttgctttctt accgatgcta tctggtttgg
     1381 ataagacagt tgcgaagata acagattata gttttatggg actgtttact aagtttttca
     1441 caaaatggga gggattttca tggaatgaaa ctctaattcc taatctaaca ctacttattt
     1501 ggattgttct tctattaact ttaattacga taactattag gaaaaagaaa atttcttaat
     1561 tgagttattt taatgattat aaacacaagt gggaaggaaa aatgaactga tctttttgac
     1621 agcaattcta cagaatagtc ttattgctat attttgattt gagtgtacga aaaaagaaaa
     1681 ataacaatag tgctcatact aattgcagaa gttttgggtg ataagataac tgataaattg
     1741 caataaaaaa tgcaacattt ttaaatctcc tctataagtg cttcaaaacc tgtcttgtaa
     1801 tccaagtatt tttggggacg gtgattaata agctagcaaa gcaccattaa ggattttttc
     1861 ggtaattgtt gccaaatcgg tttaagaaaa tactcacgaa gaagtccatt cgcattctca
     1921 ttacttcccc tttgccaaga tgaataggca tccgcaaaat aaaacagaat tcccatttgt
     1981 tcaattaaag ggtaacaagc aaactctttt tctctgtcct aagtgaaagt ctttaactat
     2041 tcttttggaa agagtcttgt gaggtgttca atagcagtca acacatacgt gaccatgttt
     2101 ttacttgaca agtgctagta gaaataatag aatagtaaaa aacctttaaa gcagtccaga
     2161 gaggcagcta aggttagacg gtgaaagggt ggagactacc catttttcgt ggaaccttgc
     2221 tgttggcagg ttcctttttt cgtggcttct gttggccaga ctctctcact agtaaaggta
     2281 aaaggagaaa cctatgcgag aacatcgtcc aatcattgct cttgattttc ctagttttga
     2341 ggcggtcaag gaatttttag ctcttttccc agcagaagaa agcctttatc tcaaggtagg
     2401 gatggagctt tattacgcag cggggcctga gattgtgtcc tacttaaaag gtttgggtca
     2461 tagtgtcttt ttggatctca aacttcatga cattcctaat acagtcaagt cagccatgaa
     2521 ggtcttgtct cagcttggtg tcgatatgac taatgtccat gcggctggtg gtgtagagat
     2581 gatgaaggcg gcgcgtgaag gtcttgggag tcaagccaaa ttgatcgctg taacgcagct
     2641 gacatcaacg tcagaagccc agatgcagga gtttcaaaat atccaaacca gtctgcaaga
     2701 gtctgtgatt cactatgcca agaagacagc tgaagctggc ttggatggtg ttgtttgctc
     2761 ggctcaggaa gtacaagtca tcaagcaggc taccaatcca gattttatct gtctgacacc
     2821 agggattcgt ccagctggtg ttgcagttgg agatcaaaaa cgagtcatga cacctgctga
     2881 tgcctatcaa atcggcagtg actatatcgt agtgggacgt cccattaccc aagctgagga
     2941 tcctgttgca gcttatcatg ccatcaagga tgaatggaca caggactgga attaaagaac
     3001 tagattagaa aaataaaagg agaataccat gacacttgct aaagatatcg ctagccacct
     3061 cttgaaaatc caagccgttt acctcaaacc agaggaaccc ttcacttggg catctggtat
     3121 caagtcaccg atttacactg ataatcgtgt gacactagcc tatccagaaa ctcgtaccct
     3181 aattgaaaat ggttttgtgg aagctatcaa agaagccttt cctgaagtag aagtgattgc
     3241 aggaactgca acagcaggga ttccacacgg agccattatt gctgataaga tggacttgcc
     3301 ttttgcctac atccgtagca aaccaaaaga ccacggagct ggtaatcaaa tcgaaggtcg
     3361 cgtagctcaa ggtcaaaaaa tggtagtggt tgaagacctt atttcaacgg gtggttcagt
     3421 tcttgaagct gtagcagcag ccaagcgaga aggagcagat gtacttggag ttgtagcgat
     3481 tttcagctac caattgccaa aagcagataa gaactttgca gatgctgatg ttaaacttgt
     3541 gacgctttca aactatagcg agcttatcca tctagcccaa gaagaaggtt acatcacgcc
     3601 agagggcctt aatcttctaa aacgctttaa agaagaccaa gaaaattggc aagaaggtta
     3661 ggtcagtaag ataaagagag acgaggctac cgagtctctt ttaccatttt atttaaaata
     3721 tgacaatatt ttaaataaaa taattgacaa cgct
//
LOCUS       DAVKZB010000132         3664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKZB010000132 DAVKZB010000000
VERSION     DAVKZB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.132"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(288..821)
                     /locus_tag="VXA80_001836"
     CDS             complement(288..821)
                     /locus_tag="VXA80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV0354280.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(910..1686)
                     /gene="recX"
                     /locus_tag="VXA80_001837"
     CDS             complement(910..1686)
                     /gene="recX"
                     /locus_tag="VXA80_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV0354281.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1724..3079
                     /gene="rlmD"
                     /locus_tag="VXA80_001838"
     CDS             1724..3079
                     /gene="rlmD"
                     /locus_tag="VXA80_001838"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0354282.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            3141..3212
                     /locus_tag="VXA80_001839"
     tRNA            3141..3212
                     /locus_tag="VXA80_001839"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3174..3176,aa:Glu,seq:ttc)
     gene            3464..>3664
                     /locus_tag="VXA80_001840"
     rRNA            3464..>3664
                     /locus_tag="VXA80_001840"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1181 a          644 c          697 g         1142 t
ORIGIN      
        1 aaaaaaatgt ttttgatacg aagttgacct gtatttttta tactaatcat tttcgtattt
       61 tttgtattaa acgatataag tttgttgtaa acttactagg aataaagaca ttaaaaaata
      121 atagtatatc tatttgtttt atatatttta cgaattctgc ataaatctct ttctagtaat
      181 gtgttgtaac tctgctataa tggatttatt cctttttgtg tttacacaat ttattttata
      241 ctaccaaaaa aacaaggtca ggattttgtt cctgaccttt gacaacttta ccgattcttt
      301 agttctacat agcgcttgta ccaaatgttt acataggctt ctgagaaagg accacgtcca
      361 ttgttaatcc aatcaacaag aattttgaca tgttctttta aaatatagtc caagtcatca
      421 gaataattca ttttgcgttt gtgacgctcg tactcttcaa cgtccaagag acgtttttcc
      481 ccatctgtaa aaattttaac atccaaatcg taatcaatat acttcagtgc ttcttcatcc
      541 agatagtagg ggctagccat attgcaatag taagaagttc cattatcacg aatcatggca
      601 atgatattaa accaatattt cttgtgaaag taaacaatag ccggttctcg agtgacccaa
      661 cgacgaccat cactttcggt aacaagtgta tgatcgttga caccaataat ggcgttttct
      721 gttgttttta gtaccatggt gtcccgccaa gttcggtgga gactcccatc atgcttataa
      781 ctttgaattg taataaagtc gccttctttt ggaagcttca taactaacca actttctaca
      841 atttataagt ttatcattta ctattgtacc ataaaattac ccaaaatctg tgaatttcac
      901 ttggaaatat taaagatatt ctctaagagc gcttgctata tccgaaaaat cgtaaccctt
      961 tcgtgctaaa acttgagtta aacgctgctt cagttcgtat ccttcatact ttcgggcata
     1021 cttagtatat tgcttatcaa gttccttgaa gatgagttcc tgagtcgttt cttcatcaac
     1081 ttgactatcc aattcgtcaa aggcaatttt agcatcaaaa taagagaagc ccttgttagt
     1141 caagttctgg ataatcttat cttgcagggc acgagctgga agttttccct catatttttt
     1201 caatagttta ttggctacac gttgagcaac ttccgaaaaa tcaaaatcat tcaagatttc
     1261 ttctatagta gattttgaaa ttcctttttg tgctaatttc tgagtcagta cataaggtcc
     1321 cttgtctcct gaaagttgat tggcattgat gatagcataa gcgtactggc tatcattaat
     1381 ccacttctct tctttaagat tagcaatgac ttgagaaacg atgttttcat taatatcata
     1441 ttttttcaga tattctctga cctctttttc agtacgtgct ttaaaggata agtggtagag
     1501 ggccagattc ttaccataag aaaattgagc aaagtcttga atctctttca attcctcttc
     1561 gcttatcacc ttatctctcg ataacataaa acgaacaatt gtgtcttcgg tgatatagca
     1621 tttgtcgcca ttatcaagct ccatcagata gagtcttttt ttcttttcaa gttttgtgat
     1681 tttcatagct ctattataac tcaaaatgtg ataagatagg ggtatgaatc tgaaagtgaa
     1741 acaaaaaata ccattaaaaa tcaagcgcat gggaattaac ggtgagggaa tcggctttta
     1801 ccaaaaaaca ttagtctttg taccaggagc tctcaaaggc gaagatatct attgtcagat
     1861 tacttctatt agacgcaact ttgttgaagc aaaattactg aaggtcaaca agaagtctaa
     1921 atttcgaatt gtgccatctt gtactattta taatgaatgc ggaggctgcc aaatcatgca
     1981 cctgcattat gataagcagc tggagttcaa gacggactta cttcatcaag cgctgaaaaa
     2041 atttgctcct gcaggatatg aaaattatga aattcgtcca actattggaa tgcaggaacc
     2101 aaaatattac agagctaagt tacaatttca gactcgaaaa tttaaaaatc aggtcaaggc
     2161 gggcttatat gcacaaaact ctcactattt agtagagttg aaagactgcc tggtacaaga
     2221 taaggaaacc caagtgattg ctaatcgctt agcagaatta cttacttatc accagattcc
     2281 aatcacggat gagagaaaag ttctaggtgt ccgtactatt atggtccgac gcgcgagaaa
     2341 gaccggacag gttcagatta ttattgttac aaaccgccag cttaatttaa ctcaattggt
     2401 aaaagagttg gttaaagatt tcccagaagt tgtgacagta gctgttaata caaatacagc
     2461 taaaaccagt gagatatatg gtgaaaagac agagattatc tgggggcaag agagtattca
     2521 agaaggtgta ctcaattatg aattttcact atcccctcga gctttttatc aactaaatcc
     2581 tgagcaaaca gaagtcctct atagcgaagc agtaaaagcg ctggatgttg ataaagaaga
     2641 ccatttgatt gacgcttatt gtggagttgg aacgattgga tttgcctttg caaagaaagt
     2701 aaaaacactc agaggtatgg atattattcc agaagctatt gaagatgcca agcgaaatgc
     2761 taaaagaatg ggatttgaca atactcatta tgaagctgga acggcagaag agattattcc
     2821 tcgttggtac aaggaaggct accgagcaga tgctttgatt gttgacccac cacgtacagg
     2881 tctggatgat aagttattag atactattct tacttatgta ccagaaaaaa tggtttatat
     2941 ttcttgtaat gtttcgacct tggctcgtga tttggtacgc ttagtagaag tctatgatct
     3001 tcattatatc cagtcggtcg atatgttccc acatacagct cgaactgaag ctgttgtaaa
     3061 attaataaaa aaagtttaaa aaaggtgttg acaaaacata aaaaggctgt ataatagtaa
     3121 gagttgaaaa taacaactca ggtccgttgg tcaaggggtt aagacaccgc cttttcacgg
     3181 cggtaacacg ggttcgaatc ccgtacggac tatggtatgt tgcgtcagga gcacttgatg
     3241 aaaaaaagtt taaaaaagct taaaaatctt caaaaaagtg ttgacaattg aaagtggctg
     3301 tgatatacta atatagttgt cgcttgagag aagcaagtga caaagacctt tgaaaactga
     3361 acaagacgaa ccaatgtgca gggcgctaca acataagttg tagtactgaa caatgaaaaa
     3421 acaataaatc tgtcagtgac agaaatgagt aagaactcaa acttttaatg agagtttgat
     3481 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac gctgaaggag
     3541 gagcttgctt ctctggatga gttgcgaacg ggtgagtaac gcgtaggtaa cctgcctggt
     3601 agcgggggat aactattgga aacgatagct aataccgcat aagagtggat gttgcatgac
     3661 attt
//
LOCUS       DAVKZB010000133         3661 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKZB010000133 DAVKZB010000000
VERSION     DAVKZB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.133"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            112..1521
                     /locus_tag="VXA80_001841"
     CDS             112..1521
                     /locus_tag="VXA80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0354283.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1539..2834
                     /locus_tag="VXA80_001842"
     CDS             1539..2834
                     /locus_tag="VXA80_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0354284.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGKNIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2839..3147
                     /locus_tag="VXA80_001843"
     CDS             2839..3147
                     /locus_tag="VXA80_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0354285.1"
                     /translation="MKQILLVCNAGMSTSILVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3144..3452
                     /locus_tag="VXA80_001844"
     CDS             3144..3452
                     /locus_tag="VXA80_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0354286.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1241 a          619 c          705 g         1096 t
ORIGIN      
        1 tcaagaaatg aggcaaacac aagatatgta tcaacaattt ttaaaattgt ctagacaaat
       61 tttaaaatta tgctatactt tatgtataga ataatagaaa ggaggttgag catgaaaaaa
      121 aagttagtat ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa
      181 ggtcaatatg gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa
      241 gattttttcg ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa
      301 gatttccact tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt
      361 cgactcatca agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat
      421 gccatcattg aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt
      481 gatttaccag tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag
      541 ttattcgtga agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg
      601 acaactttca atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat
      661 ccaaatctaa aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct
      721 agtgcaaaag tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt
      781 ttaaacttga cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga
      841 tttacagatg acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca
      901 gaaagattgg taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag
      961 cttcaactga tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa
     1021 cgtgttcaag cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac
     1081 ttcaaagagt atgaatggct ggagcgtcgc atgaatccat atcgtggttg ggaaattttt
     1141 ccgaaagcca tttatgatat tgctatgatt gtgaaggaag aatatggtaa tatcccatgg
     1201 tttatcagtg aaaacggaat gggtgttgaa aacgaagcac ggtttatcga tgaaaatgga
     1261 gttatcgatg acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa
     1321 gccattgaag agggaagtca ctgttttgga taccacgctt ggaccgcatt tgattgctgg
     1381 tcttggaata atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa
     1441 aagagaacca tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt
     1501 gaagtagaaa ttgaggagta aagagaggag atattcatat gaaaaactca aaatttatag
     1561 accaatttgc cacctttgct ggtaaactag ggaaccaaat tcatttaaaa accctaagag
     1621 atgcattcgt aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca
     1681 acacggtatt taagtggatt ttccaagggg atacattaac aagattccaa tattggggaa
     1741 taacaattgc aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt
     1801 atttcttagc taaaaacaga gatttcgaaa acccgttagc agcatcaatg ctatcattag
     1861 tttctttaat tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg
     1921 cggtaaacat ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg
     1981 gggttatcgt agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt
     2041 tacaaatgaa ccttggtaaa aatattccac cagctgttag tagatcattt agcgtattac
     2101 ttccagtcat gaccgtcatc tccttatttg gggttgtttc agcattatta ttcaatataa
     2161 ctggaatgaa cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg
     2221 gtacaagctt aatcggggtc attattattt actctttagg aaatatgtta tggctatttg
     2281 gtattcacca agcagttatt tacagtgcca tcctagaacc attactatta attaacatta
     2341 ctgaaaacat cactgcagca aataatggac aagccattcc acacatcatc aacctatcac
     2401 aaatacaaac attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa
     2461 cattcttagt gagtcgcaat gctgtctcta aaaacgtggc taaattatct tttggacctg
     2521 gtatcttcaa tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat
     2581 tagctattcc atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag
     2641 ttacaggatt catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa
     2701 atgcatggtt agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg
     2761 cacttggagt tcttctatac attccattta tcaaagttaa tgacaaagtt gttgaacaag
     2821 aaatggaagg ttaagattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca
     2881 agtatacttg ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatctctatc
     2941 caagcaaaat caatgacaga agctaaaaag aatatccatg aagcagatgt cattctaatt
     3001 gggccacaaa ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta
     3061 gataccattg atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca
     3121 cttgcatgga taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca
     3181 ttgccaatag tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag
     3241 gaaatttaaa agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc
     3301 acaagattca ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct
     3361 tgctatttat gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttactatcta
     3421 aagaattcat cgatctctat caaaataaat aggaacactg tcattcccat ctgtctttat
     3481 taatgacgac tacattgtag gaacctccaa aagttggaca aaatgattga aaggattgga
     3541 tcctgcatta cacaagatcc aatccttttc ttttactcta gtttttataa tatgttgaat
     3601 ctagaatagt ccactgcagt ttctaaaaaa tttctggaaa tggatgttaa gctccaattt
     3661 a
//
LOCUS       DAVKZB010000134         3647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKZB010000134 DAVKZB010000000
VERSION     DAVKZB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.134"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..318
                     /locus_tag="VXA80_001845"
     CDS             1..318
                     /locus_tag="VXA80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0354287.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKGAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            318..2009
                     /locus_tag="VXA80_001846"
     CDS             318..2009
                     /locus_tag="VXA80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0354288.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            2218..3624
                     /gene="lacG"
                     /locus_tag="VXA80_001847"
     CDS             2218..3624
                     /gene="lacG"
                     /locus_tag="VXA80_001847"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0354289.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
BASE COUNT         1074 a          701 c          760 g         1112 t
ORIGIN      
        1 atgaatagag aagaagtaac attgttaggt tttgaaatcg tagcctatgc tggcgatgct
       61 cgttcaaaac tattggaagc cttgaagggt gctgaagctg gtgattttga aaaagcggac
      121 gctctggtag aggaagctgg tagctgtatt gcagaggctc accacgcgca aacaagtcta
      181 ttgactaagg aagcttctgg tgaggactta gcgtatagtg tgaccatgat gcatggtcaa
      241 gatcacttga tgacaactat cttgttaaaa gacctaatgc accatttaat tgaactttac
      301 aagagaggag ttaaataatg aacaaactaa ttgcatttat cgagaaagga aagcctttct
      361 ttgagaaact atctcgtaat atctatcttc gtgctattcg tgatggtttc attgcaggta
      421 tgcctgttat tctcttctca agtatcttta tcttgattgc ctttgtacca aactcatggg
      481 gctttaaatg gtctgatgaa gttgtagcct ttctgatgaa accttatagc tattctatgg
      541 gtattctggc tctcttggta gctggtacaa cagctaagtc attgactgac tcagtaaacc
      601 ggagcatgga aaaaaccaat caaatcaact atatgtcaac attgttggca gcaattgttg
      661 gtttgttgat gttggcagct gatcctatcg aaagtggtct agctactgga ttcttgggga
      721 caaaaggttt gctttcagcc ttccttgctg ccttcgttac tgtagccatc tataaggttt
      781 gtgttaagaa caacgtcact attcgtatgc ctgacgaagt tccaccaaat atctcacaag
      841 tctttaaaga tgtgattcca ttcactctct cagttgtttc actttatgct cttgatttac
      901 tagctcgtca ttttgttggt gcaagcgtag ctgaatcaat cggtaaattc tttgcaccac
      961 tcttctcagc agcagacgga taccttggta ttaccattat ctttggtgcc tttgccttct
     1021 tctggtttgt gggtattcat ggtccatcta ttgttgaacc tgctatcgca gcgattacct
     1081 atgcaaatgc tgaagtcaac ttgaaccttc tccaacaagg tatgcatgct gataaaatcc
     1141 ttacttcagg aactcaaatg tttatcgtta ctatgggagg tactggtgcg acactggttg
     1201 ttccattcat gttcatgtgg ttaacaaaat caaaacgtaa ccgtgcaatc ggacgtgctt
     1261 cagtagttcc aactttcttt ggtgtaaatg aaccaatctt gtttggtgca ccacttgtat
     1321 taaatccaat cttcttcatt ccatttatct ttgctccaat cgcaaacgta tggattttca
     1381 aattctttat tgaaactctt ggaatgaact cattcactgc taatctacca tggacaactc
     1441 cagctccact aggtctagtt cttggaacta acttccaagt gctatcattc attcttgctg
     1501 cccttctaat cgtggttgac gttgtcattt actatccatt ccttaaggtc tatgatgaac
     1561 aaattcttga agaagaacgt tcaggtaagt ctaatgatga attgaaagaa aaagttgctg
     1621 caaacttcaa cactgcaaaa gcggatgcta ttcttgaaaa agcgggtgta gaagcagcac
     1681 aaaatacaat cactaaagaa acaaatgtcc tcgttctctg tgcaggtgga ggaacaagtg
     1741 gtctccttgc aaatgctttg aataaggcag cagcagaata caatgtccct gtgaaagcag
     1801 cagcaggcgg ctatggtgct caccgtgaaa tgttaccaga gtttgatctt gttatccttg
     1861 cccctcaagt tgcttcaaac tttgaagata tgaaagcaga aacagataag ctcggtatta
     1921 aactagcgaa aacagaaggc gctcaataca tcaaattaac tcgtgatgga aaaggtgctc
     1981 ttgcattcgt acaagcgcaa ttcgattaag gctagagact ctgaaatagt ctcccatcgt
     2041 tacggaaatc gctatggcga atttcctatt attaattcgt cggtaaaaag atatcgtttt
     2101 tacctcctca tgtcacaatt cggtgacttg gtacaagaag tgagatggag aaggatggct
     2161 cactgactcc tctcctctca cttttacttt atttaaatca agaaataggt gaaaaaaatg
     2221 acaaaaacac ttccaaaaga ctttattttt ggtggcgcaa cagctgctta tcaagcagaa
     2281 ggtgctacac atactgatgg aaaaggacca gttgcttggg ataaatatct tgaggataac
     2341 tactggtaca ctgccgaacc agctagtgat ttttacaatc gatatccagt tgacctcaag
     2401 ctagcagaag agtatggtgt caatggtatt cgaatttcta ttgcttggtc acgtattttc
     2461 ccgactggtt acggccaagt aaatgctaaa ggtgttgagt tttatcataa tttatttgca
     2521 gagtgtcaca aacgtcatgt tgagcctttt gtaactcttc atcactttga cacgccagaa
     2581 gctctccact caaatggaga cttcttaaac cgtgaaaata tcgaacattt tgtagactac
     2641 gctgccttct gttttgaaga atttccagaa gtaaactatt ggacaacctt taatgaaatt
     2701 ggaccaatcg gtgatggtca atatttggtt gggaaattcc ctccaggtat ccagtacgac
     2761 cttgccaaag tctttcaatc acaccacaat atgatggtgt ctcatgcacg cgcggtaaaa
     2821 ttgtacaaag agaaaggcta taaaggggaa attggtgttg ttcacgccct gccaactaaa
     2881 tatcctctag atcctgaaaa tccagcagat gttcgtgcag ctgagttgga agatatcatc
     2941 cacaataaat tcatcttaga cgcaacttat ctaggtcgct attcagctga aaccatggaa
     3001 ggtgtcaacc atatcttatt agtcaatggt ggtagtttgg atcttcgtga agaagatttt
     3061 acagcgttag aagctgcaaa agacttgaat gatttcctag gaatcaacta ctatatgagt
     3121 gactggatgg aagcctttga tggagaaact gaaattatcc ataatggtaa aggtgaaaaa
     3181 ggaagctcta agtatcaaat caaaggtgtt ggtcgtcgtg tagctcctga ctatgtacca
     3241 cgcacggatt gggattggat tatctaccct caaggtttgt atgaccaaat catgcgtgtg
     3301 aagaaagatt atcctaacta caagaagatt tacatcactg aaaatggtct cggctataaa
     3361 gatgagttcg ttgataacac tgtttacgat gatggtcgta ttgattacgt gaagcaacac
     3421 ttggaggttt tatctgatgc gattgcagat ggagctaatg taaaaggtta cttcatttgg
     3481 tcattaatgg atgtcttctc atggtcaaac ggttatgaga aacgttatgg tctcttctac
     3541 gtagattttg aaactcaaga acgttatcct aagaaatcag ctcactggta caagaaagta
     3601 gcggaaactc agattataga ctagtagaat tagtcattag atataga
//
LOCUS       DAVKZB010000135         3636 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKZB010000135 DAVKZB010000000
VERSION     DAVKZB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.135"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            93..320
                     /locus_tag="VXA80_001848"
     CDS             93..320
                     /locus_tag="VXA80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354290.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            608..3494
                     /gene="nanA"
                     /locus_tag="VXA80_001849"
                     /pseudo
     CDS             608..3494
                     /gene="nanA"
                     /locus_tag="VXA80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
BASE COUNT         1235 a          614 c          830 g          957 t
ORIGIN      
        1 tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa cagatagaga
       61 gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc aagacaaaaa
      121 agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt tttctgattg
      181 atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt ctgattgtga
      241 gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag ctatttgaaa
      301 aagtgttcga taaggattga gcaggaagta tggtgtaaat agcataagct gatgtccatc
      361 atttgcttat aaagagatag tttagtttaa ttgcagcggt gtcctggtag ataaactaga
      421 ttggcaggag tctgattgga gaaaggagag gggaaatttg gcaccaattt gagatagttt
      481 gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaaattaat aaaagacaaa
      541 ctaagtgcat tttctggaat aaatgtctta tttcagaaat cgggatatag atatagagag
      601 gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta ggaaactatc
      661 ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc ctgttttagc
      721 tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg gggagagctc
      781 aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt ctggcaataa
      841 gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt actatgcacg
      901 agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca atgcttcaaa
      961 tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg aaaacgcaac
     1021 agttcacatg gagtttaagc cagatgccaa ggctccagca ttctataatc tcttttctgt
     1081 gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata atactgctac
     1141 tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg atgcaccctt
     1201 aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac cgacagcaga
     1261 attgccaaat ggtcgagttc gcctctacgt aaacggggta ttatctcgaa caagtctgaa
     1321 atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg gagcaaccaa
     1381 gcgtgccaac aatacggttt gggggtcaaa tctacagatt aggaatctca ctgtgtataa
     1441 tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac gctcagattt
     1501 agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat tcgaaagcgg
     1561 gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag cacttctcaa
     1621 gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt cgagtgactg
     1681 gggtgatatc ggtatggtca tcagacgtag tgaagataat ggtaaaactt ggggtgaccg
     1741 agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga tcggttcacc
     1801 agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct tttctatcta
     1861 tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag aagaagccta
     1921 caaagaaatc gatggaaaaa cctatcaaat cctctatcgt gagggagaaa aggaagctta
     1981 taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag actatcgcgt
     2041 tgttgtagat cctgttaaac cagcctatag tgacaagggt gatctataca agggtgacca
     2101 attactagga aatatctact tcacaacaaa caaaacttct ccatttagaa ttgccaagga
     2161 tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag cgcctcaaga
     2221 tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc ctggaacagg
     2281 aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt atacgactaa
     2341 taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag atgatcatgg
     2401 aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg gtcaaaagat
     2461 ccactcttct acgatgaaca atgaacgtgc gcaaaataca gaatcaacgg tggtacaact
     2521 aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc aggttgctac
     2581 aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac aggttaaaga
     2641 tgtctatgtt caaatgtctg ctatccatac gatgcacgaa ggaaaagaat acatcatcct
     2701 cagtaatgca ggtggaccga aacgtgaaaa tgggatggtc cacttggcac gtgtcgaaga
     2761 aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt ttgcctataa
     2821 ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata ctgaaaaagg
     2881 acaaaatgcc tataccctat catttagaaa atttaattgg gactttttga gcaaagatct
     2941 gatttctcct accgaagcga actagagaga tgggcaaagg agagatgggc aaaggagtta
     3001 ttggcttgga gttcgactca gaagtattgg tcaacaaggc tccaaccctt caattggcaa
     3061 atggtaaaac agcgactttc ctaacccagt atgatagcaa gaccttgttg tttgcagtag
     3121 ataaggaaga tatcggacag gaaattattg gtatagctaa aggaagcatc gaaagtatgc
     3181 ataatcttcc tgtaaatcta gcaggtgcca gagttcctgg cggagtaaat ggtagcaaag
     3241 cagcggtgca tgaagttcca gaatttacag ggggagttaa tggtacagag ccagctgttc
     3301 atgaaatcgc agagtataag ggatctgatt cgcttgtaac tcttactaca aaagaagatt
     3361 atacttacaa agctcctctt gctcagcagg cacttcctga aacaggaaac aaggagagtg
     3421 acctcctagc ttcactagga ctaacagctt tcttccttgg tctgtttacg ctagggaaaa
     3481 agagagaaca ataagagaag aattctaaac atttgatttt gtaaaaatag aaggagatag
     3541 caggttttca agcctgctat ctttttttga tgacattcaa gctgatacga aatcataaga
     3601 ggtctgaaac tactttcagg gtagtctgtt ctataa
//
LOCUS       DAVKZB010000136         3572 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKZB010000136 DAVKZB010000000
VERSION     DAVKZB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.136"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(180..335)
                     /locus_tag="VXA80_001850"
                     /pseudo
     CDS             complement(180..335)
                     /locus_tag="VXA80_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(520..1131)
                     /gene="rpsD"
                     /locus_tag="VXA80_001851"
     CDS             complement(520..1131)
                     /gene="rpsD"
                     /locus_tag="VXA80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV0354291.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1326..2378)
                     /locus_tag="VXA80_001852"
     CDS             complement(1326..2378)
                     /locus_tag="VXA80_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0354292.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2375..3073)
                     /locus_tag="VXA80_001853"
     CDS             complement(2375..3073)
                     /locus_tag="VXA80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354293.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(3240..>3572)
                     /locus_tag="VXA80_001854"
     CDS             complement(3240..>3572)
                     /locus_tag="VXA80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0354294.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT         1013 a          779 c          676 g         1104 t
ORIGIN      
        1 aggtgtacta ttctagtttc aatccattat atttcgtctg atgggcaaat cttataaaga
       61 gattatagaa cttttatagt agtttgaaat aagatgtgaa caactctatc aggaaagtca
      121 aattaattta tagaaatatt ttagcagcca aggtgtactg ttatagattc aatacactat
      181 acttggtggt ttagctctcc tgttttctct tttagcttta accagccata aatggtatta
      241 cgtgagattt gaaaaacgtg tgatgcttct gttatactac ctgttcgctc acaataagag
      301 agaacttttt tacgaaaatc tattgaatat gccataaaag attataccac attgtgtact
      361 atttttggtt cattttacta tatcactttg gactatatcg gtctagttat gtctaaacga
      421 ttatagctgg atactaagag aaatcaaaaa agcactaagg gagaacgtcc caaagtgctt
      481 aatatcaagg cctcaaagcc tatcttattt aataaaatat tacaacattt tgttgtagaa
      541 ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg tcgcgttctg gcaagcgagt
      601 caatgaacct tccaactttt cagcgtcgaa tgatacgaat gctggacgtc caagagtagc
      661 ttctactgct tcaaggattg ctggaacttt caatgatttt tcacgaactg agatcacttg
      721 acctggagtt acgcggtatg atgggatatc aacgcgtttc ccgtcaacaa ggatgtgacc
      781 gtggtttacg aattgacgag cttgacgacg agtagtcgcg agaccaagac ggtaaacaac
      841 gttatccaaa cgacgttcca aaagaagcat aaagttgaaa cctaggattc cgcctttgat
      901 ttttgtagct tgtacgaaca agttacggaa ttgtttttca cctacaccgt aagtgaaacg
      961 aagtttttgt ttttcagcca attgcaaacc gtattctgac aatttagaac ggttgtttgg
     1021 tccgtgttgt cctggtacgt agttacgacg tgccaattct ttacctgtac ctgtaagtga
     1081 aaggccaagg cgacgagctt gtttccaaga tggtcctgta taacgtgaca tatgaatgtc
     1141 ctcctgatat aaataatatt tcggtggaaa tagtcactta gaaagccctg attcgtgcag
     1201 atgcccttcg cctaaacagc caaggttact tgtcataaga cacctgttga cgagcttcat
     1261 gctttcctgc tgatatttca cacaaaatct attctaccat gaatagctgg atttgtaaag
     1321 gggttttagg ctttattttc actaccagag aggttgagaa cgagggtaaa ggtacttcct
     1381 agaccgtact ggctgctgac tgtgatttcc ccacccaatt gatgggccaa ttcacgcgca
     1441 atcgcaagtc ctaatccatg accacctgtc ttcatgttac gcgaagtttc gacacgataa
     1501 agacgtttga aaatattttc caaatcctct ggggcaatac cctgcccttc atcggtcaca
     1561 ctgattgaaa gctggtcctt ctccagctta gccaccactt ccagcttggt tcctggagca
     1621 gaatatttaa aagcgttatc gaccagattc accaagatac gagaaagctt agcataatct
     1681 ccctcaatcc gggcagactc tgggattacc tgcaagtgga catctcttct ctcctgctca
     1741 atcaaaaact gaaattcact catgcactca attaagagct tgtccagaaa aatactgtct
     1801 ttactggtag tttccacctg atttctagct gtgtttaggg tcaaaaaatt caactcctca
     1861 accagtttat tgagcctctc cgtctggcgt ccaatggttg ctagataatg agcttgctcc
     1921 gactccttga taatcccatc caaaatccct tctaccgtcg cttgaatcga agtgataggg
     1981 gtcttaatat catgcgacaa ctgggcaatc atcaagccct tttctcgttc gctttcttcc
     2041 aaggaatcaa agcttacctg caaatcatgg gacatctcat taaaagtttg ccctaattgc
     2101 tgaaattcta caggaccttg aacctccaaa tttgaaggaa aatccttggc cgctacccgc
     2161 ttggcatgct ccttgagttt gcccaacgac gtaaagactg gcaataggag aaagagacta
     2221 atcccagcac cgacaaggct ggcaacgatg gtcatcccaa gcaaaaagta aatctcgcct
     2281 ttcgcaatca gcattttttg aacagcccaa aaaacaacca aaatggttaa gagggttgaa
     2341 ataatatatc caaccaaaat ataacttttt agtttcatgt ttgtcctctc ggtttctcta
     2401 tcttatatcc caacccccaa actgtcttaa tagtgggcgt ttggtcacta ctatattttg
     2461 ccagctcctg tcgaagagca tggatatgca cattcaaggt attggtgtca tccacgtagt
     2521 cttctttcca gatcttttca tagaggtctg tcttggagaa aactcgctct ggattgctag
     2581 ctaaaatcca cagcaattca aatgatttaa cagttaaatc cagcatttct tctcctattt
     2641 gaacttcatg actactatga ttcattttta gattgccaag ggaaatcagc tctgtttcgc
     2701 ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa aactagctca cgtgggctaa
     2761 aaggcttagc aataaaatca tctgctccca agctcaggcc gtaaatcttg tcctgttcac
     2821 tggtcttagc agtaataaat aggaaaggct gctctggtga taagtattga acctcactga
     2881 ttaaatcata accatccatc cgaggcatca tgacatctgt gataatcaaa tcaatcggtt
     2941 ttttcttgaa cagctctagc gcttccagtc catcatgggc taccaagacc tgataacctg
     3001 cctgaattaa gtatctctga tgaatatctg tgatttctac ctcgtcgtca acgagtaaaa
     3061 ttgtctttcc catctgtctc tcctttgata aaaacagtgc tatactgctt taagtataac
     3121 actattttta ttgttttacg atgattttaa tctgctattg aaagaaagtc ctgattgctg
     3181 attgagttgt taaactaatc ttagtcagcc cactatccat catttatttc cttagctgat
     3241 tagttttctt cttttttgtt tttcaaacct agaccaccga gtaaacctgc aagcgcaagc
     3301 ccaaggaaac caatacttgc cattgatgtt tgagtctcac cagtatttgg tagcatagct
     3361 ttatcctctg acatcatcgt atcagacatc ttgttagcag aagcagccat gttttcacct
     3421 gccatcgtgt tggtagaact tgtcatggtg tcagcaggca tgctatctgt agtacctgta
     3481 gcatgattgt gattcatcgg agtcacgcca gaaccagagt tagaaggtga taatgaacca
     3541 tttgctgtgt ctgatgtttc tttaacattt at
//
LOCUS       DAVKZB010000137         3500 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKZB010000137 DAVKZB010000000
VERSION     DAVKZB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.137"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            106..921
                     /locus_tag="VXA80_001855"
     CDS             106..921
                     /locus_tag="VXA80_001855"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV0354295.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            940..1581
                     /locus_tag="VXA80_001856"
     CDS             940..1581
                     /locus_tag="VXA80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV0354296.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1612..2613
                     /locus_tag="VXA80_001857"
     CDS             1612..2613
                     /locus_tag="VXA80_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV0354297.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISALEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKETYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            2623..3252
                     /locus_tag="VXA80_001858"
     CDS             2623..3252
                     /locus_tag="VXA80_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV0354298.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
BASE COUNT         1053 a          760 c          719 g          968 t
ORIGIN      
        1 acaaatcttt ttataaataa aaatatttat ttattgacaa aaccggttta ctagaatata
       61 atttggttat agagaagtct taacaaattt gaaaggaagt atcaaatgac aaatacatca
      121 ttctcaattg agcagttttc tttaaaagga aaaattgctc tcatcaccgg cgcttcttat
      181 ggaattggat ttgctattgc caaatcctac gctgaagccg gcgctactat tgtctttaac
      241 gatatcaatc aagatctggt caataaaggg attgaagctt atcgtgaagt tggcatcgaa
      301 gcccatggat atgtctgtga cgtgacagac gaggacggta tccaagccat ggtcaagcaa
      361 atcgaacaag aggttggtgt cattgacatc ctcgttaata acgctggtat tatccgccga
      421 gttccaatgt gcgaaatgag cgccgctgat ttccgtaagg tcatcgatat tgacttaaac
      481 gcaccattta tcgtttcaaa ggcagttatt ccttctatga taaagaaagg gcatggaaag
      541 attatcaata tttgttcgat gatgagcgaa ctgggacgtg aaacagttag cgcttatgct
      601 gctgctaaag ggggcttgaa aatgttgacc cgcaacattg cgtctgaata cggtggagcc
      661 aatatccaat gtaacggaat tggaccaggt tatattgcca ctcctcaaac agcacctctt
      721 cgtgagttgc aagaagatgg ttctcgccac ccatttgacc agttcatcat tgcaaaaaca
      781 cctgctgcac gttggggaaa tcctgaagat ttgatgggcc ctgctgtctt tctcgctagt
      841 gatgccagca attttgtcaa tggccacatc ctatatgtag atggcggtat cttagcctac
      901 atcggaaaac aacctgagta aaaatagaaa gaagatctta tgaaaatcgc attaatcaat
      961 gaaaatagtc aagctagcaa gaatcacatt atttacgata gtctaaaaga agcgacagat
     1021 aaaaaagact accaattatt taactatggt atgcgtggag aagaaggaga aagtcaatta
     1081 acttatgtgc agaacggact aatggctgcc atccttttaa atacaaaggc agttgacttt
     1141 gttgttaccg gctgtggtac gggtgtaggg gctatgcttg ctttaaacag cttccctggt
     1201 gttgtctgtg gtctagcagt ggacccaact gacgcttacc tttattctca aatcaatggt
     1261 ggtaacgcct tgtctatccc ttatgccaaa ggatttggct ggggggcaga actgaccctc
     1321 aaattgatgt ttgaacgctt atttgctgaa gaaatgggcg gtggctaccc aagagaacgt
     1381 gtaatccctg aacaacgcaa cgctcgtatc ttaaacgagg tgaaacaaat cacccacaat
     1441 gatttgatga ccatccttaa aacaatcgac caagacttcc tcaaagacac catctctggc
     1501 aaatacttcc aagaatactt ctttgaaaac tgccaagatg atgaagtcgc tgcttatttg
     1561 aaagaagtat tagccaagta aagctattct aaaccagaaa ggaactaatg gatgacgaaa
     1621 atattactgt ttggcgaacc attaattcga atctcaccat tagatgccac cagtatcggc
     1681 gatcatgttg ccagttcgac ttattttggc ggatcagaaa ttaacatcgc ttgtaatttg
     1741 caagccctgg gtatctcaac gaaagtcttt accgcactcc ctgccaacga gattggagat
     1801 cgttttctca cattcttgaa acagcaccaa atcgatacca gttcaatctg tcggcttggc
     1861 gatcgaatcg gcctctacta tttggagaac ggctttggtt gtcgtcaaag tgaagttttc
     1921 tacgatcgta agcatacgag tatcagccag attcggccaa acatgctaga tatggattct
     1981 ctctttcagg ggattagcca ttttcatttt agtggaatca ccatagctat cggtcaagag
     2041 gtccgtgcga tccttctcct actcttggaa gaagccaagc gccgaggaat tgtcgtttca
     2101 atggatctca atctgagaac aaagatgatt tcagccctag aagccaagta tgaattttct
     2161 aagtttgcac gttttactga ctattgcttc ggtattgatc ctctcatgat tgatgaccaa
     2221 aatctagaga tgtttccaag agacagtgct agcctagaag aggtggaaaa tcgcatgcga
     2281 cttttaaaag aaacctatgg tttcaaggct attttccata ccctccgatc tagtgatgag
     2341 caagacaaaa atgtctatca agcctatgct ctggaagaac gatttgaaga gtctgtccaa
     2401 ctaaaaactg cagtctatca acgaattggt agcggggatg cctttatatc tggtgccctt
     2461 taccaactac tccatcattc ctccctaaaa actaccattg actttgcagt tgcgagcgca
     2521 actctcaaat gcactcttcc aggagaccat ctctccactt ccgcaactag tattgaaaat
     2581 ttactggcaa atgcacaaga tatcattcgt taggagaatt acatgaccaa atcagatacg
     2641 attattgaac taaaaaaaca gaaaattgtc gctgtcattc gaggaaatac aaaggaagaa
     2701 ggactacaag cctcgattgc ttgtatcaag ggcggtatca aagctattga aatcgcctat
     2761 accaatcagt atgcaggaca aatcatcaag gaacttgtag acttgtatca ggacgatcag
     2821 agtgtttgta tcggtgcagg tactgtgctt gatgccgtaa ctgctagaga tgccattcta
     2881 gctggagcaa attacgttgt ttctccatct ttccatgctg aaactgcgaa aatgtgcaat
     2941 ctctacagca caccgtacat tccaggctgt attaccctca cagagatcac gactgcactt
     3001 gaagccggta gtgaaatcat caaactcttc ccaggtagta ctctcagtcc agcatatatc
     3061 tctgcagtca aggcaccgat cccacaagtt tccgtaatgg taaccggagg agtcggccta
     3121 aacaacatcc ctcaatggtt cgctgctggt gcagatgccg ttggaattgg tggcgaactc
     3181 aataaactcg cttcccaagg caactttgac cgcatcagcg agattgccca acagtatgtt
     3241 acactcagat aaaatcataa ctacccgtct aacgggtggt ttatctcaga gctataagcc
     3301 caaatcatca gccagcgcct aaagacgctg gctttcacgt tgttcaagcc ttattgctct
     3361 tgactcgtca cttgcctctt taagagactt tggtattact taccactatc cctaaaggga
     3421 tcctcatatt cttttacact caatttatct agtgctatag tagattgaaa ctggaatagt
     3481 acacctctac ttctaaaaca
//
LOCUS       DAVKZB010000138         3393 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKZB010000138 DAVKZB010000000
VERSION     DAVKZB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.138"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            307..2808
                     /locus_tag="VXA80_001859"
     CDS             307..2808
                     /locus_tag="VXA80_001859"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV0354299.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDTITSPEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            2947..>3393
                     /locus_tag="VXA80_001860"
     CDS             2947..>3393
                     /locus_tag="VXA80_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354300.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIY"
BASE COUNT         1042 a          657 c          725 g          969 t
ORIGIN      
        1 agattcagtc tactatacgt tcttcataaa tcaaaaagcg acataggttg tcggctattt
       61 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
      121 ctatacgaga taagcgtctt gacttactcc ttgatttact gcataacaat gggataaaaa
      181 gtgggagata gagcaattca tagtcatcaa aattaatgag atacagtata cagttttttc
      241 tttaaacaca tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga
      301 gagaccatga gtttttataa tcataaagaa attgagccta agtggcaggg ctactgggca
      361 gaacatcata catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat
      421 atgttccctt atccgtctgg agctggtctg cacgtaggac acccagaagg ttatactgca
      481 accgatatcc tcagtcgtta caaacgtgcg caaggctaca atgtccttca cccaatgggc
      541 tgggatgctt ttggtttgcc tgcagagcaa tacgctatgg atactggtaa tgacccagca
      601 gaatttacat cggaaaacat tgccaacttc aaacgtcaaa tcaatgcgct tggattttct
      661 tatgactggg atcgtgaagt caacacaaca gatccaaact actacaagtg gactcaatgg
      721 attttcacca agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg
      781 gttgaggaat tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgag
      841 cgtggaggct atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct
      901 tacgcagagc gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat
      961 atgcaacgca actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga
     1021 acagacaagg aatttacagt cttcactact cgtccggaca cacttttcgg tgcgactttc
     1081 actgtcttgg ctcctgaaca tgaattagta gacactatca caagtccaga gcaagcagaa
     1141 gctgtagcag actataaaca ccaagccagc cttaagtctg acttggctcg tacagacctt
     1201 gctaaagaaa aaacaggggt ttggactggt gcttatgcca tcaaccctgt caatggtaag
     1261 gaaatgccaa tctggattgc ggactatgtt cttgctagtt atggaacagg tgcggttatg
     1321 gctgtgcctg cccacgacca acgtgactgg gaatttgcca aacaatttga ccttccaatc
     1381 gtcgaagtac ttgaaggtgg agatgtcaaa gaagctgcct acacagagga tggcctgcat
     1441 gtcaattcag acttcctaga tggactcaac aaagaagacg ctattgccaa gattgtggct
     1501 tgcttggaag aaaaaggctg tggtcaggag aaggttacct accgtctccg cgactggctc
     1561 tttagccgtc aacgttactg gggtgagcca attccaatca ttcattggga agatggaact
     1621 tcaacagctg ttcctgaaac tgaattgccg cttgtcttgc ctgtaaccaa ggatatccgt
     1681 ccttcaggta ctggtgaaag tccactagct aacttgacag attggcttga agtgactcgt
     1741 gaagatggtg tcaaaggtcg tcgtgaaact aacaccatgc cacaatgggc tggttcaagc
     1801 tggtactacc tccgctatat tgacccgcac aatactgaga aattggctga tgaggacctc
     1861 ctcaaacaat ggttgccagt agatatctac gtgggtggtg cggaacatgc tgtacttcac
     1921 ttgctttatg ctcgtttctg gcataaattc ctctatgacc tcggtgttgt tccgactaag
     1981 gaaccattcc aaaaactctt taaccaaggg atgattttgg gaacaagcta ccgtgaccac
     2041 cgtggtgctc ttgtggcaac cgacaaggtt gaaaaacgtg atggttcctt ctttcatata
     2101 gaaacagggg aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt
     2161 gttaacccag acgatgtggt ggaacaatac ggtgccgata cccttcgtgt ttatgaaatg
     2221 tttatgggac cactcgatgc ttcgattgct tggtcagaag aaggtttgga aggaagccgt
     2281 aagttccttg accgagttta ccgtttgatt acaagtaaag aaatccttgc ggaaaacaat
     2341 ggtgctcttg acaaggctta caacgaaaca gtcaaagctg ttactgagca aattgaatct
     2401 ctcaaattca acacagctat tgcccaactt atggtctttg tcaatgctgc taacaaggaa
     2461 gataagcttt atgttgacta tgccaaaggc tttattcaat tgattgcacc atttgcacct
     2521 cacttggcag aagaactctg gcaaacagtc gcagaaacag gtgagtcaat ctcttatgta
     2581 gcttggccaa cttgggacga aagcaaattg gttgaagatg aaattgaaat tgtcgtccaa
     2641 atcaaaggaa aagttcgtgc caaactcatg gttgctaaag atctatcacg tgaagaatta
     2701 caagaaatcg ctttagctga tgaaaaagtc aaagcagaaa ttgacggtaa ggaaatcgtg
     2761 aaagtaattg cggtaccgaa taaactcgtt aatatcgtcg ttaaataacg agtttattag
     2821 ctctatctgc caccttcaat agtccactgg actattgaag ccaactaaat tagttaacat
     2881 tgttgtgaaa taaaaagaag taactctaga gtatgaactt tagagttact tttagaatag
     2941 gtcagaatga atagattagt gaacgatagc aggaagtgtg aaattttatt atttaagatt
     3001 tctattattg tagtgtcagt tatagtatta gtaatgtcaa tagtgggtgc atattatttt
     3061 aaaaattgtc tttgggacaa ggaaagttta aatttgacac tcactttaga cactctcttt
     3121 gcagttttta ttactttctt tagtttttca aatgataaat caagcaatat ttttggaata
     3181 tctccaaatg aaattataag gacacagtct gaagtgtatc aatttttatt tacaaaatct
     3241 tttgcaactc tattaatagt gacaaatttg ttatccatta ttcattattt tggagccaaa
     3301 aatttaatag tttcttttaa tgaaacttat cctcgtattc aatccagtgt tggtatcatg
     3361 tggctaccac tgtttggtat tctgataatc tat
//
LOCUS       DAVKZB010000139         3389 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKZB010000139 DAVKZB010000000
VERSION     DAVKZB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.139"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(130..384)
                     /locus_tag="VXA80_001861"
     CDS             complement(130..384)
                     /locus_tag="VXA80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV0354301.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(404..2245)
                     /gene="typA"
                     /locus_tag="VXA80_001862"
     CDS             complement(404..2245)
                     /gene="typA"
                     /locus_tag="VXA80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV0354302.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2407..3132
                     /locus_tag="VXA80_001863"
     CDS             2407..3132
                     /locus_tag="VXA80_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0354303.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSFSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(3172..3369)
                     /locus_tag="VXA80_001864"
     CDS             complement(3172..3369)
                     /locus_tag="VXA80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354304.1"
                     /translation="MGILSIILGLLFPIVGLILGIIGLVFSISYQKESQLDYKIEKIL
                     NILGIVISVVNWIVAIALIFR"
BASE COUNT         1016 a          744 c          635 g          994 t
ORIGIN      
        1 gatgccatta acgaatttta ttcaagtttt tcccattcat actatacaag taaaagagat
       61 ggtgttaact aaaaagcaat tcaaactatt gtaaaattcc tagcaaaaag agagccgaaa
      121 ctctcttttt tatcttcttt tacttttttt gactggcatg agtgtgatgt ctctaacact
      181 aaagtaagct aggatcaaca tggctattgc taggaatatt tctgttggta attgaaaaat
      241 tttcagaaaa gatagaacca ataaaatcaa gagtgccact aaaatacata ccatagcgac
      301 gatattgaca gtccctttaa tgctttctgg tgtcgcaaat acatagagta ggagcagtaa
      361 aattcctagg actaaataga ccatctttct ctctttctag ctcttattca gctgattttt
      421 tcttcttgtt agctttctca cgctctgctt tgttaaggat ttgtttacgc aaacggatag
      481 actcaggcgt tacttccatg tactcatcgt cgttcaagaa ctcaagagac tcttcaagtg
      541 tcaagatacg aggcgtcttg ataacagctg tttggtcctt agtagctgaa cgaacgttgg
      601 tcatttgttt tgccttcgtg atgttaactg tcaagtcatt ttcacgagag ttttcaccga
      661 tgatcattcc ttcataaacc tcagtacctg ggttgacaaa gatcgtacca cgttcttcga
      721 tagacatgat tgagtaagtt gtagccttac cagcatcgat agaaacaagg gcaccacggt
      781 gacgtccacc aatttctcct ggaatcaatg gcaagtattg gtcgaaggta tggttcatga
      841 taccgtaacc acgagtcatt gacaagaact cagttgagta tccaatcaaa ccacgcgctg
      901 gaacaaggaa gaccaaacga gtttgaccat taccagttga aatcatatcc aacatttcac
      961 ctttacgttc agaaaggctt tggataacag acccttggta ttcttctgga gtgtcgattt
     1021 gtacacgttc aaatggttca catttaacac cgtcgatttc ttttacgata acttctggac
     1081 gagatacttg aagttcatag ccctcacgac gcattgtttc gataaggatt gacaagtgca
     1141 attctccacg tcctgaaaca gtccatttat ctggtgaatc agttgggtca acacgaaggg
     1201 aaacgtctgt ttgcaattct gcctgcaagc gttcttccac cttacgagag gttacccatt
     1261 taccttcttt accagcaaat ggtgagttgt tgaccaagaa agtcatttga agagttggct
     1321 catcgatgtg taggattgga agagcttcta ctgcatctgt cggagtgatg gtttcaccga
     1381 caaagatgtc ttccatacct gaaacggcaa tcaagtcacc cgctttggct tcttggattt
     1441 cacgacgttc caaaccaaag aaaccgaaga gttttgtaac acggaagttt ttagttgtac
     1501 cgtcaagttt agaaagggta acttggtccc caaccttaac tgtaccacgg aagacacgac
     1561 cgataccgat acgtccaacg aagtcattgt agtccaaaag cgacacttgg aactgcaaag
     1621 gctcatctga gttatctact ggagctggga tatggtcgat aatcgtgtca aagattggtg
     1681 ccatagtcgc ttcttggtca gctggatcat ctgacaatga agaagttccg ttgatcgctg
     1741 aagcataaac cactgggaaa tcaagctggt cgtcatctgc accaagctcg atgaaaagtt
     1801 ccaagacttc atccactact tctactggac gagctgatgg cttatcgatt ttgttaacaa
     1861 ccacgattgg gacaaggtct tgttccaagg cttttttcaa tacgaaacga gtttgtggca
     1921 tggttccttc ataggcatct acgaccaaga caacaccgtc aaccattttc atgatacgct
     1981 caacttctcc accaaagtcc gcgtgtcctg gtgtgtccat aatgttgata cgagttccgt
     2041 tgtaagcaac ggcagtattt ttagcaagga tggtaatccc acgctctttt tcgatatcgt
     2101 ttgagtccat agcacgctct gccaattcag tacgtgcatc aagcgtttct gattgtttca
     2161 ataattcgtc aaccagggtt gttttaccgt ggtcaacgtg ggcgataatc gcaatgttac
     2221 ggatatcttc tcttaatttt gtcatgattt cctctataat attcaaaatt tattttctaa
     2281 ctgaacgatt ataccataat ttcaagtaaa taacataact caagcaagtg taaatgtttt
     2341 cactctgctt ttcttttcac gtcaagcctt ttcaaagcga gcgacttatg ataagatagg
     2401 cacagtatgc gtttagataa tttattagcc caagaaaaaa tcagccgaaa ggccatgaag
     2461 caagcactcc tcagagggga aattctagtc gatggttgcc cagcccgctc cctagctcaa
     2521 aatatcgata caggactaca agaactcctt tttcaggatc gaatcattca aggctatgaa
     2581 cacacctatc ttatgcttca taaacctgct ggtgtcgtta cagccaacaa agacaaggaa
     2641 cttccgaccg tcatggacct gcttccatct gacatccagt ctgacaagct ctatgccgtc
     2701 ggccgactgg accgagatac aacaggactc cttctcttga ccgataacgg tcccttgggc
     2761 ttccaactcc tccatcccca atatcatgtc gataagactt accaagtcca ggttaatgga
     2821 cttctaacac ctgaccatat ccaaaccttt caaaaaggaa ttgtcttttt agatggcact
     2881 atctgtaaac ctgcaagact agagattcta tcagcaagtc cttccttcag tcaagcctct
     2941 atcaccattt cagaaggaaa atttcatcaa atcaagaaaa tgttcctctc ggttggtgtt
     3001 aaggtgacta gcctcaagag aatccaattt ggggacttca cattgaaccc agatttagca
     3061 gaaagtaact accgcccttt gaaccaaaaa gagttacaaa tcattaaaaa ctatttagag
     3121 atgagtcgat aaaacaaaaa aagctttaaa actaaaactt ttttctttta tttaccgaaa
     3181 aattaaggcg attgctacaa tccagttaac tacagaaatc acaattccta agatattaag
     3241 aatcttttct attttatagt ctaattgtga ctctttttgg tatgaaatag aaaagaccaa
     3301 tcctatgata cccaaaatca ggcctacaat tggaaataac aaaccaagaa taatcgacaa
     3361 gatacccaca aaaagtggat ttttcttct
//
LOCUS       DAVKZB010000140         3374 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKZB010000140 DAVKZB010000000
VERSION     DAVKZB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.140"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            115..2748
                     /gene="polA"
                     /locus_tag="VXA80_001865"
     CDS             115..2748
                     /gene="polA"
                     /locus_tag="VXA80_001865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV0354305.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2833..3270
                     /locus_tag="VXA80_001866"
     CDS             2833..3270
                     /locus_tag="VXA80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV0354306.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          911 a          597 c          866 g         1000 t
ORIGIN      
        1 atctaggttt actaaattct ctggctctat ctatttattc ggaaaaagtt tgaaaaatac
       61 ttgctctagc tcttcccaat ggtatttttt gattctttcc tttataatgg gtgtatggat
      121 aagaaaaaat tattattgat tgatgggtct tctgtagctt ttcgggcgtt ttttgcgctg
      181 tatcagcagt tggaccgttt taagaatgcg gctggtttgc ataccaatgc gatttatggt
      241 tttcagttga tgttgagtca tttgttggag cgggttgagc cgagtcatat tttggtggct
      301 tttgatgcgg gaaagacgac cttccggaca gagatgtatg cggactataa gggtggtcgg
      361 gccaagactc ctgatgagtt tcgtgagcaa tttcctttca ttcgtgagtt gctggatcat
      421 atggggattc gtcactatga gctggctcag tatgaggcgg atgacatcat tgggacgctg
      481 gataagctag cagagcagga tggttttgat attaccattg tcagtgggga caaggatttg
      541 attcagctga cggatgagca tacggtggtt gaaatttcca agaaaggtgt ggctgagttt
      601 gaggccttta cgccagatta cctcatggaa gaaatgggcc tcacaccagc tcagtttatc
      661 gatctcaagg cgctcatggg tgataagtcg gataatatcc ctggggtgac caaagtcggt
      721 gaaaagacgg gtattaagct cttgctggag catggttcgc ttgaggggat ttatgaaaat
      781 attgatggaa tgaagacttc taagatgaag gaaaatctca tcaatgacaa ggaacaggcc
      841 tttttatcta aaacactagc gaccattgat accaaggcac cgattgcgat tggtttagag
      901 gacttggtct atagtggtcc agatgttgaa aatcttggga aattctacga tgagatgggc
      961 ttcaaacagc taaagcaggc tttaaatgtg tcgtcagctg atgtgtctga gagtttggat
     1021 tttactattg ttgaccaaat cagtcaagat atgctgagtg aagagtctat cttccacttt
     1081 gagctttttg gtgagaatta ccatacggat aatttggttg gatttgcctg gtcttgtggg
     1141 gataagctct atgccacaga caagcttgag ctgttgcaag acccgatttt caaggatttc
     1201 ttagaaaaaa catctctgag agtttatgac tttaagaagg ttaaagttct tttgcaacgt
     1261 tttggtgtgg atttgcaggc gcctgctttt gacatccgtt tggctaaata cctcctttcg
     1321 actgtggagg acaatgaaat tgcgaccatc gctagtcttt atggtcagac ttacttggtt
     1381 gatgatgaaa ctttctacgg taagggtgtt aaaaaggcca ttcctgaacg tgagaaattc
     1441 ttggaacact tagcttgtaa acttgctgtt ttggtagaaa cagagcctat tttacttgaa
     1501 aaactcagcg aaaatgggca attagagctt ctttatgata tggagcaacc tctggctttt
     1561 gtccttgcca agatggaaat tgctgggatt atggtcaaga aagagacctt gcttgagatg
     1621 caggctgaaa atgagcttgt cattgaaaaa ctgactcaag agatttacga gctggctggt
     1681 gaggagttta atgtcaactc gcctaagcag ttgggcgtgc ttctctttga gaaattggga
     1741 cttcctctag aatacactaa gaaaaccaag acaggttatt cgacagcagt ggatgttttg
     1801 gagcgtctcg ctcctattgc tccgattgtt aagaaaatcc tggattaccg tcaaattgct
     1861 aagattcaat ctacttatgt aattggcttg caggactgga ttttggctga tggaaagatt
     1921 catactcgct atgtgcagga tttgacccag accgggcgtt tgtctagtgt ggatccaaac
     1981 ttgcaaaata ttcctgcccg attggaacag gggcgcttga ttcggaaggc ttttgtgcca
     2041 gagtgggagg atagtgtgct actcagctct gactattcac agattgaatt gcgcgttttg
     2101 gcgcatattt ctaaggatga gcacttgatt aaggccttcc aagagggggc agatatccat
     2161 acttcgacag ccatgcgggt ctttggcatt gagcgtcctg atgatgtgac tgcaaacgac
     2221 cgtcgcaatg ccaaggcagt taactttgga gtggtttatg ggatttcaga ctttggcttg
     2281 tctaataatt tgggaattag tcgtaaggaa gccaaagcct acattgatac ctactttgaa
     2341 cgttttccag gtattaaaaa ctacatggat gaagtggtgc gggaggcgcg tgataagggc
     2401 tatgtagaga ccctctttaa gcgtcgccgt gagttgccag atatcaattc gcgcaacttc
     2461 aatattcgtg gttttgcgga gcgaactgct atcaactcac ctatccaggg ttcggcagca
     2521 gatattctca agattgccat gattcagctg gataaagcct tggttgcagg tggttatcag
     2581 actaagatgc tgttacaagt gcacgatgaa atcgtccttg aagtgcctaa atctgaattg
     2641 gtagagatga aaaaattggt gaaacaaacc atggaagaag ccattcaact cagtgttcct
     2701 cttatcgcag atgagaatga aggggcaacc tggtacgagg ctaaataaaa agggggctag
     2761 tcctcctttt ttgtagtata attctgcaaa ccttttcaaa atgtgctata ctgatgaaaa
     2821 aggaggattt ctatgagtca agaatttatc aatccaagtg atggcgtgat tcgtcagtat
     2881 ctcgcaacga gtaaaaccct tgctgtggtg ggtttgtctg accgtgaaga aacaaccagc
     2941 aatcgagtaa caaaggaaat gcaggctcgg ggctataaaa tcatcccagt caatcccaag
     3001 gcggcaggtg gcgaaatctt gggtgaaaag gcttatgcta gcctcgctga aattcctttt
     3061 cctgtagata ttgtcaatgt ttatcgtcgc agtgaatttc tgcccgatgt ggcgcgtgat
     3121 tttctcaaag ctgatgccaa gattttttgg gcacagctag gacttgaaag tttagaagcg
     3181 aaagagattt tgcgtgatgg tggatgtgat gatatcgtga tgaatcgctg tatcaagaga
     3241 gaacatactc gcttgattga ggaagcataa gaaaaaggta gctgatgggc taccttttgt
     3301 gttatactca atgaaaatca aagagcaaac taggaaacta gccgcgggct gctcaaaaca
     3361 ctgttttgag gttg
//
LOCUS       DAVKZB010000141         3368 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKZB010000141 DAVKZB010000000
VERSION     DAVKZB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.141"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VXA80_001867"
     CDS             complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VXA80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV0354307.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAKAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLS"
     gene            complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VXA80_001868"
     CDS             complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VXA80_001868"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV0354308.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2893..3156)
                     /locus_tag="VXA80_001869"
     CDS             complement(2893..3156)
                     /locus_tag="VXA80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV0354309.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          885 a          726 c          638 g         1119 t
ORIGIN      
        1 actcaaaggc agtttgtaga gctgtttcaa gagactatca atgtcattac tttgacttgc
       61 ttgactgtta gtgttgctgt tgttgcttgt gttagtattt gtagttcctg gattgcttac
      121 agaaggtacc cagtttgttc ttgaagtgtt atcgctattt tgtcggcgat aggttcttga
      181 atttgacaga tttccccgac cagataggaa ggcttttgca gcagctaact cgctagctga
      241 taactcattc ttaggaatgt aatggtaatg gtcgccgtga ggaacgatat aagcatcacc
      301 agtatcctct atgatatcag aagcattaaa gatataacca tcatctgtag tatagcgtcc
      361 ttgcgaacgt gccaaggcaa cagcaccatc gtttcttgga gtcccacctt cacgatgttg
      421 actatgttct tgtttttgcc gattgatttc ctcttttgta cggacgttat ccgcgtgggc
      481 agcatcctta aggtaaacat agtattttcc atctaccttg ataacatatc cacccttgac
      541 ctcattaaca atatcctcat cttttagctt atagtttgga tctttcatga gtaattcttc
      601 actgaaaatc gcatcataag gaaccttacc attgtaataa tgatagtggt cgccatgtga
      661 agtgacatag ccttggtctg ttatcttgat gacgatttgc tcagcattga ttccttcacg
      721 cttgctaacc tcatcaggag tcaaattctc cgttttttgc gtcgcttgtt ttccatctat
      781 ataggaaaca cgattatttt ccttaaccgt tctagcttga tacagtccca actcgtaaga
      841 acaaacactt aaaatcaaag ctgccgcaga accaacaagg tatttcttat taattttcat
      901 cgaaactctc tttctatttg atatttctag ttgcaattca tttctaaatt gcagaaactc
      961 ctctctatta cttaactggt taatagttta ctcctaaatc tccaacttgt caagaatttt
     1021 atgaaccatt taagtatttt ttgttctgac tactactttt tcaaggttgg actttctata
     1081 agaaagattt tcttgaaaat tctttttaaa atatcaaaaa aatccctgta actgagttgc
     1141 agggactttt cgattaatca aaattaacgt attctttttg aagttcaaga acttcttcca
     1201 ttgttgagca ttctgtaagg gcacggtttg cgtactcttc catcttagct gtgtcgagtt
     1261 tcttcatcaa gctgcgtgta cgaagtacag acgttgctga catagagaac tcatccaagc
     1321 ccattccgac aagaagtgga acagcttgtt ggtcaccagc catctcacca cacataccag
     1381 cccatttacc ttcagcgtga gctgctttga tcacattgtt aatcaagcgt aggattgatg
     1441 ggttgtatgg ttggtaaagg tatgaaactt gttcgttcat acggtctgct gccattgtat
     1501 attggatcaa gtcgtttgta ccaattgaga agaagtcaac ttctttagca aattggtctg
     1561 caagcatagc cgctgcagga atctcgatca tgataccaac ttggatgtta tccgcaactg
     1621 caacaccttc agcaagaagg tttgcttttt cttcatcaaa gactgctttc gctgcacgga
     1681 attctttcaa gagcgcaacc attgggaaca tgatacgcaa ttgaccgtga acagacgcac
     1741 gaagaagagc acggatttgt gtgcggaaca tagcatctcc agtctcagag atagagatac
     1801 gaagagcacg gaatccaagg aatgggttca tttcgtgagg catatcgaag taaggaagtt
     1861 ccttatctcc accgatatcc attgtacgaa cgacaacagg tttaccgttc attccttcaa
     1921 gaacagcctt gtatgcttca tactgctcat cttcagttgg gaagtcttga gaatccatgt
     1981 acaagaactc tgtacggtaa agtccaacag cttctgcacc gttgttgtta acaccttcaa
     2041 cgtcttttgg agtaccaata ttggcagcca actcgaagtg tttaccgtca gcagtcactg
     2101 tttgagcatc tttcaaaagt gcccattcag ctttttgttt cgcataggct tcaccagctg
     2161 ctttaaattc tgccgcttgt tcatctgttg ggttgataat cacttctcca gtgatcccgt
     2221 taacagcaag gatgtcaccg tctttaacga tttcagtgat gttgtttgta cctaatacag
     2281 cagcaatttc aagtgtacgt gccatgatag ctgagtggct tgtacgtcca ccaatgttgg
     2341 ttacaaaagc ttttacaaag tttttgtcca attgagctgt atctgaagga gtcaagtcat
     2401 gcgcaatcac aatcacttct tcattgatag aagctgggtt tggcaatttt ttaccaagaa
     2461 ggtttgccaa tacacgtttt gtcacgtcgc ggatatccgc tgcgcgttct tgcatgtatg
     2521 ggttgtcttc catgccttca aagatagtga taaacatatc tgtaacttct ttcagacctg
     2581 cttctgcatt cactttcttc gcacggatag tttccttgat ttggctgatc atttctgggt
     2641 cagcaagaac cattaagtga gcatcaaaaa cttgagctgc ttcttcaccg agcgtaccta
     2701 ctgctttctc gcgaataaca gaaagctcgt cttgtgatgc ctgtagagcg gcatcaaggc
     2761 gagcttcttc tgcgtttgta tcttcgactg taatagtctc aaatgacaaa tccggctgaa
     2821 cgagtagata tgcttttgca actgcaacac cgtcagatgc tgcgattcct ttaagcattt
     2881 ctgtcatttc ccttatgcca atccttcttt ttccattgtt tctgagattg cagcgatagc
     2941 gtcatctgca tctgcacctt cagctgagat agttacgtca gcaccttggc caacaccaag
     3001 actcataaca cccataattg atttaaggtt aactgattta cctttgtact caagagtgat
     3061 atctgaagca aatttgctag cagtttgtac caacaatgtt gctggacgtg cgtgaatacc
     3121 tgtttctgcc actacgtgga aatctttaga agccatagtt tgactctcct tttgttcttt
     3181 ctttttttga gttatatgtg ataaccctta caatttagta ttataccatc ttctaggatt
     3241 tttttcaagc attttctgga ttttccttca atttcctttc agataacttg ctgaaaatct
     3301 tctatcctag ataacaaaac acaggatata gtttctttaa aaacaattta taggataatc
     3361 attgctat
//
LOCUS       DAVKZB010000142         3363 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKZB010000142 DAVKZB010000000
VERSION     DAVKZB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.142"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(45..1994)
                     /gene="mutL"
                     /locus_tag="VXA80_001870"
     CDS             complement(45..1994)
                     /gene="mutL"
                     /locus_tag="VXA80_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV0354310.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLASA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            2350..2790
                     /locus_tag="VXA80_001871"
     CDS             2350..2790
                     /locus_tag="VXA80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354311.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLIEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            2787..3245
                     /locus_tag="VXA80_001872"
     CDS             2787..3245
                     /locus_tag="VXA80_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV0354312.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          964 a          798 c          668 g          933 t
ORIGIN      
        1 cgtttttctg attttgaaca tttttcccag acttttttat acttttaata tttccccaac
       61 tcacggagac tggtgtgatt ttcctgaata cgtcggaaca tcttttccat atctgacttg
      121 gtaaaatgca ccaaaacagg acgtccgtga ggacagttat agggattgtc acattgagaa
      181 agctgataga ggagttgtct agctgaatga tcatcaatac gatgattggc cttgatagat
      241 cgcttgcaag acatcatgat agccagctct gctcggtatt tcttgataga aacttccttg
      301 gtcaaaagga gcatgtcgca catctcatag atgcctgatt caatctcttc ttctgccatc
      361 caaataggat gttcacgtag aataaattga ttttctccgt actctgctag aaagacgccc
      421 acttcctcta agagaggcat tctttccttg agacgcaggg catcatccgc aggaaattca
      481 aagatatagg gcactaggag ttgctgctgg ctctggtcaa cattgccaat gctttcacgg
      541 tactcctcgt acttgacccg ttcctgagca gcgtgctgat ctatgatgta aagtccatct
      601 cgcccttggg caaagagata agtcccgtgc atttgtccga aaaactccaa ctctgggaag
      661 ctggatgctt cttctcgctc cagtttgtca taagccttat cgatgctagc aagatctaac
      721 tctggatggt ctagctggtc gtagttagca ggctttctct ctgcaaaatg cagttttgct
      781 ggcttggtca atcggtccaa ggtttccttg gcaaacaggg tcaaatcctg cccttcatca
      841 gtcaattcta cctgataatc agctacttca gtttgactag gtcttgacgg ctcagttttc
      901 tcatagtaga gcgtattttc tttgagtggg agaatagttt gctccacctt ctcacgattg
      961 cgcacggtcg atttggcaag attttccaag gcatctggaa tcaaggtttg ttccttgaga
     1021 ctatttgcaa tagcttctga aaccagagtc atcagttctt tttccttgga aatccgcacc
     1081 tcttgcttag ttggatgcac attgacatcc gctagataag ggtcgatatg gatgtgaatg
     1141 acagccagtg gaaaacgtcc aaccataagc ttgcttccaa aaccatccaa aatagcacga
     1201 ttgagcagga agttcttgat ataacggcca ttgatgaaga ggctgatata attgcggtta
     1261 gcccgagtca actcaggcaa ggacacaaaa cctgaaattt cgaaatctag gtcagagttc
     1321 tcaatttcaa tcatcttctt ggcactcgcc aaaccgtaaa tccctgcgat tgcttggcgc
     1381 aattgaccag tccctgctgt tcgcgtcatt tccttgccat cactaatcaa gctaaaagaa
     1441 atctcaggat gggccaagcc cagacggttg acaatatcaa tgatatgaga caactccgct
     1501 tgctggctct tcatatactt gagacgggca ggcgtgttga aaaagagatc ctccacacaa
     1561 accttggttc ccacaggact agtcgctggg atgacttcct caacttcacc cccacgcgcg
     1621 actaacttgg ttccatgact agcaccatcc accgccgtta acagagtcaa gacactaaca
     1681 gacgcaatag aaggcaaggc ttcaccacga aaaccaagcg tccgaatccg aaagagatct
     1741 gcttgatttt ttatcttact ggtcgcatgg cgacgcaggg ccaactccac ctcatcgtgg
     1801 gcaattccat aaccgttatc tgtgatttgg attttcttga gaccagcttc ctcaatctca
     1861 atgataatct gactagagcc cgcgtcaatg gcattttcta ccaactcttt aaccacactt
     1921 gcaggacgtt caatgacctc tccagccgca atctggtttg ccagcatctc tggcaattca
     1981 ataatatgag acatctttca gcccctttct gtatctattt tcctagaaat tgattagtgc
     2041 tattatacca tatttcagaa agaacagaaa agctccacca cataggagcc caatccctcc
     2101 acatagacaa agtaccttgc cagagggagt aaaatagtgg tatactcaat aaaaatcaaa
     2161 gagcaaacta gaaagctagg cgcagacagc tcaaaacact gttttgaggt tgtagataaa
     2221 actgacaaag tcaacaacta tcctacggca agacgacgct gacgtggttt gaagagattt
     2281 ttgaagagta ttaataaagg tgtacaagaa gtgagattaa ttttgtatga aaaacaagga
     2341 gagaaattta tgaaagtaga attacagatt agtgagactt acgaggagga aaagctgatt
     2401 gtccaagcac ctcagccgac agataaagtt cagaaagtca tcgagttcgc agaaaatctg
     2461 gaccaaagag aaaaaatcaa agggaaaatc gatgatcagg tctatctagt tgagattggc
     2521 aagattcagc gcttctatat cgagaatcgg aaggttctag cagaaaccgc gagtcagacc
     2581 tacagcattg atttgcgact ctatcaggtt cttaaactct tgccaaccaa ttttatccaa
     2641 atttcccaat cagaaatcat caatatcgat tccatctctc acctcaagct cacgccaaac
     2701 ggtctgatag aaattttctt gaaaaacgaa agcttcacct actcttcacg ccgttatcta
     2761 aaaaccatca aggagaaatt agaactatga aaaaacaagt atttcacgat gcagctaccg
     2821 gtgttcttat cggcctcatc ctctctatcc tcttttcact catttatgca ccaaatacct
     2881 acgcaccact aaatccctac tctctcatcg gccaagtgat ggatcagcat caggttcacg
     2941 gtgccctggt cttgctctac tgcacactta tctgggcaac catcggtatg ctcttcaact
     3001 ttggcaaccg cttatttagc cgtgactgga gcatgcttcg tgccactctg actcatttct
     3061 tccttatgct ggctggcttt gtcccactag caactcttgc tggttggttc cctttccact
     3121 ggattttcta cctccagctc attatcgagt ttgcgattgt ctatctcatc atctgggcta
     3181 ttctctataa aagagaggct aaaaaagtag atcacatcaa tcaactcttg gagcatagaa
     3241 aataggaacc tcataataag caaatccgag aaagaaactt ctcggttttt ctttatcctg
     3301 ctgcacctta ctatagtgaa atgaaataag aacaggataa atcgatcagg acagtcaaat
     3361 cga
//
LOCUS       DAVKZB010000143         3343 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKZB010000143 DAVKZB010000000
VERSION     DAVKZB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.143"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            14..1039
                     /locus_tag="VXA80_001873"
     CDS             14..1039
                     /locus_tag="VXA80_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0354313.1"
                     /translation="MYNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNYS
                     DIFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFGA
                     AQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKVSV
                     FLKSDEMEMIRKAMVQMQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDKVNLLE
                     KMVESLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVAICK
                     DSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDFI
                     NIFEKIK"
     gene            1049..1363
                     /locus_tag="VXA80_001874"
     CDS             1049..1363
                     /locus_tag="VXA80_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV0354314.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            1394..1900
                     /locus_tag="VXA80_001875"
     CDS             1394..1900
                     /locus_tag="VXA80_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354315.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            1980..3326
                     /gene="celB"
                     /locus_tag="VXA80_001876"
     CDS             1980..3326
                     /gene="celB"
                     /locus_tag="VXA80_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV0354316.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
BASE COUNT         1056 a          481 c          648 g         1158 t
ORIGIN      
        1 aggattagat gatatgtata acttttcttc agatttccaa ctaattgaag gattgataac
       61 tcatctaatg acactacaag tacgtttaga aagccggatt accttaaata accctttagt
      121 agatgaaatt aaacagaatt atagtgatat attctttatg actagagaaa ttcttgcaaa
      181 tatggacaag ttcttggagt ggacaatatc agatgatgaa gtagcatatg taagtcttca
      241 ctttttagct gctatggaac gtagcaaaga gagtactaag tttaatattc ttgctatttg
      301 tgcaacagga tttggtgcag cccaaatgtt aagaaatcgt ttggaaacgg agtttgggaa
      361 gcgagtagaa gttgttgatg taataggcta ttatgaattg aatcaagaaa aacttaaagg
      421 aatagatttt atagtgtcag ctgttgattt atcaaatcta tattttcaaa tcccagtttt
      481 taaagttagt gtgtttctta agagtgatga gatggaaatg attagaaaag ctatggttca
      541 gatgcaagtt tctagttatg tacaatcatc aaagattaac aaatttgaaa acaacggctt
      601 tagacagtat tttagtaaag aaaacttttt aatatgtact gaatcagaca aggtaaattt
      661 acttgaaaag atggttgaga gtttatctgt tggtgaatca aatgagtttg aacaatcttt
      721 actttatgga attaaacagc gcgaagaatt aagttcagtt gttttttctg aaaaaattgc
      781 agttccgcat cctattcagc cctttggtac agagggaaag gtttcggttg caatttgtaa
      841 agattcgtta ctctgggata atcaaagttc acatgttcag ctagtatttc ttctatctcc
      901 atcaatatat gggaacgaag gtttggcaac tgtgactaaa aagatagtct ctctgacaga
      961 gaatgatgaa ttgcaaaatc agctaatatc ttgcaataac tttgaagatt ttattaacat
     1021 atttgagaag ataaaatagg aggttcatat gaatcaagag gaactgcagg ttgcagcatt
     1081 tgaaattatc ctgcattcgg gaaatgcaag gtcagaaata catgaagcat ttgcaaaaat
     1141 gagagaaggt agttttgatg atgcagaaag taaattaaat caatcaaatg agataattct
     1201 tgaagcgcat catgcacaaa ccaaactctt gcaagaatac gcaagtggag tggaaattaa
     1261 aattgaaatt atcatggtac atgctcaaga tcatttaatg accactatga cattattaga
     1321 agtagcaaag gaaatgttag ctctttataa gaaagttaac taaatatgtg ttgttatata
     1381 aggaggaaat gatatggata aaaaagaacg acaaaagatt gaacaacaac gtcgagagat
     1441 ggctctaact aatacttttt tcaatcgata tcttcttcta cgttattcaa tagcactctt
     1501 cttttttggg aatatttatt ggcttttgaa tcagtttatt agtccctcgc ctattattat
     1561 tttccccatt atgctcatag tattttctat tttagcaact attgaacaat ttaaacttta
     1621 tggaaatagg aaagaaaagt tgggaataac actaatgttt gttagaatac aaatacttat
     1681 ttcaatagga ttacttgttt taacttggac aagttggttt aagaatcttt ttccgatttt
     1741 tgaaaataac caagtagcac gtttatttgt ttttgtagtt cttctattag gtttagttct
     1801 gagtttgtta gacattagaa gaattaaaaa aatttataaa cgaacagata aggcttatca
     1861 acaatttgtc caattggaaa agaactcact tagtttataa tcctgatcta gtataatttg
     1921 tatttgaaac tagtctttag aaagtaaaaa ttattatgtt ttattaagga ggtctattta
     1981 tggatcaaca aaacgggttg tttggttttc ttgaaaacca tgttatggga ccaatgggca
     2041 aacttgctca gtttaaagta gtgcgtgcta tcacggctgc aggtatggct gctgtaccat
     2101 ttactattgt aggatcaatg tttttggtat tcagtatttt gccacaagct ttctcatttt
     2161 ggccaattgt ggcagatatt ttctctgctt catttgataa attcacatca ctttacatgg
     2221 ttgcaaacta tgcgactatg ggttctctat ctctttattt cgttctatca cttgcatatg
     2281 aattgacaaa aatttatgca gaggaagaag aactcaatat gaatcctctt aatggtgcct
     2341 tgctcgcctt gatggctttt gtcatgacag taccgcaaat catttttgat ggtggaatga
     2401 tgaagactgt gacaagtcta aaagaaggtg cagtaattgc agatggatgg gcaatgggaa
     2461 atggagtcgc acgttttggg acaacaggga tttttaccgc aatcattatg gcaattgtga
     2521 ctgttcttat ttatcgtatg tgtgttaaac ataattgggt tattaaaatg cctgaagctg
     2581 ttccagaagg agtttctcgt ggatttaccg ctttggttcc gggatttgtt gttgcatttg
     2641 ttgttatctt tatcaacggt cttcttgtag caatgggaac agatattttt aaagtcattg
     2701 caattccatt tggttttgta tccaatctga ctaattcgtg gattggttta atgattattt
     2761 atctactgac tcaactactt tggattgtag gtatccacgg tgcgaacatt gtttttgcat
     2821 ttgttagtcc aattgctctt gctaacatgg ctgaaaatgc tgctggcggg cacttcgctg
     2881 ttgcaggtga attttctaat atgtttgtaa ttgcaggtgg ttctggtgca actttaggac
     2941 tatgtttata tattgctttt gcctctaaat ctgaacagct taaagcaata ggacgagcat
     3001 ctgtagttcc agccttattt aatattaatg aaccattaat ttttggatta cctattatct
     3061 ataatccagc cttggctata ccatttattt tagcaccaat ggttactgct actatttatt
     3121 acgtagcgaa ttctctaaac tttattaagc caattatcgc acaggttcca tggccaactc
     3181 cagtagggat tggagctttc ttagggacag cagatcttcg agctgtatta gttgctctag
     3241 tatgtgcatt tgcagcattc ctagtctatc ttccattcat ccgtgtatat gatcaaaaat
     3301 tggtgaaaga agagcaaggt atctaataag taaaaaagta tat
//
LOCUS       DAVKZB010000144         3284 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKZB010000144 DAVKZB010000000
VERSION     DAVKZB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.144"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            43..3162
                     /gene="phtE"
                     /locus_tag="VXA80_001877"
     CDS             43..3162
                     /gene="phtE"
                     /locus_tag="VXA80_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV0354317.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1220 a          542 c          636 g          886 t
ORIGIN      
        1 aagttgagaa aaaacttcat catttattga aatgagggat ttatgaaatt tagtaaaaaa
       61 tatatagcag ctggatcagc tgttatcgta tccttgagtt tatgtgccta tgcactaaac
      121 cagcatcgtt cgcaggaaaa taaggacaat aatcgtgtct cttatgtgga tggcagccag
      181 tcaagtcaga aaagtgaaaa cttgacacca gaccaggtta gccagaaaga aggaattcag
      241 gctgagcaaa ttgtaatcaa aattacagat cagggctatg taacgtcaca cggtgaccac
      301 tatcattact ataatgggaa agttccttat gatgccctct ttagtgaaga actcttgatg
      361 aaggatccaa actatcaact taaagacgct gatattgtca atgaagtcaa gggtggttat
      421 atcatcaagg tcgatggaaa atattatgtc tacctgaaag atgcagctca tgctgataat
      481 gttcgaacta aagatgaaat caatcgtcaa aaacaagaac atgtcaaaga taatgagaag
      541 gttaactcta atgttgctgt agcaaggtct cagggacgat atacgacaaa tgatggttat
      601 gtctttaatc cagctgatat tatcgaagat acgggtaatg cttatatcgt tcctcatgga
      661 ggtcactatc actacattcc caaaagcgat ttatctgcta gtgaattagc agcagctaaa
      721 gcacatctgg ctggaaaaaa tatgcaaccg agtcagttaa gctattcttc aacagctagt
      781 gacaataaca cgcaatctgt agcaaaagga tcaattagca agccagcaaa taaatctgaa
      841 aatctccaga gtcttttgaa ggaactctat gattcaccta gcgcccaacg ttacagtgaa
      901 tcagatggcc tggtctttga ccctgctaag attatcagtc gtacaccaaa tggagttgcg
      961 attccgcatg gcgaccatta ccactttatt ccttacagca agctttctgc cttagaagaa
     1021 aagattgcca gaatggtgcc tatcagtgga actggttcta cagtttctac aaatgcaaaa
     1081 cctaatgaag tagtgtctag tctaggcagt ctttcaagca atccttcttc tttaacgaca
     1141 agtaaggagc tctcttcagc atctgatggt tatattttta atccaaaaga tatcgttgaa
     1201 gaaacggcta cagcttatat tgtaagacat ggtgatcatt tccattacat tccaaaatca
     1261 aatcaaattg ggcaaccgac tcttccaaac aatagtctag caacaccttc tccatctctt
     1321 ccaatcaatc caggaacttc acatgagaaa catgaagaag atggatacgg atttgatgct
     1381 aatcgtatta tcgctgaaga tgaatcaggt tttgtcatga gtcacggaga ccacaatcat
     1441 tatttcttca agaaggactt gacagaagag caaattaagg ctgcgcaaaa acatttagag
     1501 gaagttaaaa ctagtcataa tggattagat tctttgtcat ctcatgaaca ggattatcca
     1561 agtaatgcca aagaaatgaa agatttagat aaaaaaatcg aagaaaaaat tgctggcatt
     1621 atgaaacaat atggtgtcaa acgtgaaagt attgtcgtga ataaagaaaa aaatgcgatt
     1681 atttatccgc atggagatca ccatcatgca gatccgattg atgaacataa accggttgga
     1741 attggtcatt ctcacagtaa ctatgaactg tttaaacccg aagaaggagt tgctaaaaaa
     1801 gaagggaata aagtttatac tggagaagaa ttaacgaatg ttgttaattt gttaaaaaat
     1861 agtacgttta ataatcaaaa ctttactcta gccaatggtc aaaaacgcgt ttcttttagt
     1921 tttccgcctg aattggagaa aaaattaggt atcaatatgc tagtaaaatt aataacacca
     1981 gatggaaaag tattggagaa agtatctggt aaagtatttg gagaaggagt agggaatatt
     2041 gcaaactttg aattagatca accttattta ccaggacaaa catttaagta tactatcgct
     2101 tcaaaagatt atccagaagt aagttatgat ggtacattta cagttccaac ctctttagct
     2161 tacaaaatgg ccagtcaaac gattttctat cctttccatg caggggatac ttatttaaga
     2221 gtgaaccctc aatttgcagt gcctaaagga actgatgctt tagtcagagt gtttgatgaa
     2281 tttcatggaa atgcttattt agaaaataac tataaagttg gtgaaatcaa attaccgatt
     2341 ccgaaattaa accaaggaac aaccagaacg gccggaaata aaattcctgt aaccttcatg
     2401 gcaaatgctt atttggacaa tcaatcgact tatattgtgg aagtacctat cttggaaaaa
     2461 gaaaatcaaa ctgataaacc aagtattcta ccacaattta aaaggaataa agcacaagaa
     2521 aactcaaaac ttgatgaaaa ggtagaagaa ccaaagacta gtgagaaggt agaaaaagaa
     2581 aaactttctg aaactgggaa tagtactagt aattcaacgt tagaagaagt tcctacagtg
     2641 gatcctgtac aagaaaaagt agcaaaattt gctgaaagtt atgggatgaa gctagaaaat
     2701 gtcttgttta atatggacgg aacaattgaa ttatatttac catcgggaga agtcattaaa
     2761 aagaatatgg cagattttac aggagaagca cctcaaggaa atggtgaaaa taaaccatct
     2821 gaaaatggaa aagtatctac tggaacagtt gagaaccaac caacagaaaa taaaccagca
     2881 gattctttac cagaggcacc aaacgaaaaa cctgtaaaac cagaaaactc aacggataat
     2941 ggaatgttga atccagaagg gaatgtgggg agtgacccta tgttagatcc agcattagag
     3001 gaagctccag cagtagatcc tgtacaagaa aaattagaaa aatttacagc tagttacgga
     3061 ttaggcttag atagtgttat attcaatatg gatggaacga ttgaattaag attgccaagt
     3121 ggagaagtta taaaaaagaa tttatctgat ctcatagcgt aaggaatagc agtagaaaaa
     3181 gtctgaatca aaaatgaagt tctctcaaaa gttagaaata aaactctgac tttgggagaa
     3241 tttcatttta ttattaatat ataaaatttc ttgacataca actt
//
LOCUS       DAVKZB010000145         3217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKZB010000145 DAVKZB010000000
VERSION     DAVKZB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.145"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..1116)
                     /locus_tag="VXA80_001878"
     CDS             complement(<1..1116)
                     /locus_tag="VXA80_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0354318.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTILKRYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQ"
     gene            complement(1129..2967)
                     /locus_tag="VXA80_001879"
     CDS             complement(1129..2967)
                     /locus_tag="VXA80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0354319.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(2985..>3217)
                     /locus_tag="VXA80_001880"
     CDS             complement(2985..>3217)
                     /locus_tag="VXA80_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV0354320.1"
                     /translation="FITHIKLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQV
                     AMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1076 a          649 c          436 g         1056 t
ORIGIN      
        1 ttgatcttta gcacctaatc cgttttccac tataaataat gggatttgat aacggtcata
       61 atatctattt aaaattatac gtagtccaat tggatcaatt tgccatcccc actcagaaga
      121 ctctaaataa ggatttacta agccaccaat aatattccct tctcctgaat tatactgtgt
      181 tggaagagca gattgagtca cactcatgta atagctaaag gataaaaaat ctacggtata
      241 attttttaat aactctgcat cttcagctgc aaactctatg ttaatgtcat gttccttaaa
      301 atatcttttt gcataattcg gataataacc tctaacatgc acatctgaaa atagataatt
      361 tagattctca tactcatgag tcgcccatac atcttttgga tttggagtca ttggataagc
      421 tggcatagct aataccatac accccacctt aaactctgaa ttaatctcac gagcaatttt
      481 tgtaaccaaa ctcgaggcga ctaattcatg atgtatagct tgatataatt cttgtttcga
      541 aagattctcc ttaggtatat ctattcctcc actagtaaac ggtaattcca aaacagagtt
      601 tacttcgtta aatgtaagcc aatatttaac tttatcttta taccttttta aaattgttcg
      661 agcaaatttt tcataaaaat gaatcattct cctatcaatc catccatgat attttcttgc
      721 taaatataat ggagtctcat agtgtgaaag agttacaagt ggttctatcc cgtgagcatg
      781 tagttcatca aataattcat cataatattt caaaccagct tcgttaggtt cttcctcatc
      841 tccttttgga aaaattctac tccatgcaat agaagtacga aaaacattaa agcccatttc
      901 agaaaacaag gatatatctt ccttatattt atgataaaaa tcaataccta tcaattttaa
      961 gttatcttct gtaggatttt ctgttgcttc tcctaatcca cctttgggta acacatcctg
     1021 aactgataag cccttaccat cttcattata tgctccctct acttgattag ctgcaacagc
     1081 tccaccccaa agaaaatcat ctggaaaaat ggtcataact ttcctccatt ataatattac
     1141 cagtaattcc ttagaatgct cgattgtctg attattaggt aatactaata catctagaaa
     1201 atcattggta ttcgttacaa ttactggtgt aactgtttcg tagcctttag tcttgattaa
     1261 attcaagtcc atttcaaaaa tcaactgatt tttgaaaact ctgtctcctt cttctacatg
     1321 actaataaaa ccttgacctt ttagctcaac agtatctaat ccaatatgaa ttagtaactc
     1381 aacaccctca tcactcttca atccaattgc gtgcttagtc ggaaaaatat ttgtaatttt
     1441 cccatcaaat ggtgcataaa ccttaccttc acttgggata atcgctactc cgtctccaat
     1501 tagtttatct gaaaatgttt tatcctggac atcgcttaac ggaatgattt ctcctgatat
     1561 aggagaaaat atcatttttt tatttgaaac tccagcttta acttctaaat tactagaact
     1621 ctcttcttca tcgattccaa atatataagc taatacaaag gtaataacaa ccgaaatgac
     1681 cgccacaatt aaagcattta caatatttga tggtacatca gaataaataa attgaggcaa
     1741 cgctatcaaa gatgggacag caaatagata cgctttaaca ctagtaagac ctgcaaataa
     1801 tcccgctaat ccaccaccaa tcatagctgc ataaagcggt tttttatatt ttaaagtcac
     1861 accatataat gcaggttcgg taattcctgc aagtaaggct gagaaacctg ctgcaaaagc
     1921 aatttgtttt gtattattat ttttactctt taatgcaaca gccatcgaag cagccccttg
     1981 agctaagttt gaccctaaca ttgctggaag aattaatacg tctggagtag caatagatgc
     2041 cgccaaaaaa ataggtgcaa aagcccaatg cattccagtc ataacaataa atggcataat
     2101 agcaccaaga atagctaatg taagccatcc agctacacca tacatttgcc caactagatt
     2161 tgataatcct tcaccaacaa ttactccaat aggtccgact acaactaagg caatacagct
     2221 tgatactaat aatactagcg taggttgcaa aaaactctta gtaacagctg gtgttaattt
     2281 agcaatcatt ttttcaatat atttcatcaa ccaaaccata ataagaattg gaacgactga
     2341 cgaaccataa ctagctggtg tcacaggtac accaaataaa ctaagaggat tccctgattg
     2401 caccatttga acaaaatttg gatggagaag tacacctgct acagacatag ctaatgtaga
     2461 tgttactttt aatttttgtg atgcagaata agctaataac agcggtaaga aataatatgg
     2521 agcatcccca aaaaatgtca aaaaagcaat agtctgagaa tctgattgca atataccaag
     2581 cattggtaaa atgattacca agactttcaa catacctccc cctaacattg ctggaatgat
     2641 tggagtcatg gaaccagcga tatactcaat gattctttct aaaatattcc ctttgtaccc
     2701 ttgattaact gaatcggatt caaaattgcc aagtttaacg aattctttat aataattagc
     2761 tacatcatta ccaagtataa tttgatattg tccattcttt ttcataatac ctattacacc
     2821 tggtatcttc ttcacatcat catcattgac taaattttca tcttttaatt ctaatcttaa
     2881 acgtgttaca caatgggtaa ctctattgac atttttttcg cctccaatta catcgaggat
     2941 tttttgtacc gtatctttat aactcatggt attctcctat tctattaatc taaatttttt
     3001 gttaagcgac gaatatgagc catcaaataa actaattcac tagaagtcag caaataattg
     3061 tactccgttt gtataaacat tgccacctgt tcaccacatt catattctct aggatattta
     3121 tttttcatta atgctaacaa gtcttcatca tcatcgtcac aataagttgt attttcaacc
     3181 aagcgatggg caaataattt tatatgagtt atgaatc
//
LOCUS       DAVKZB010000146         3214 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKZB010000146 DAVKZB010000000
VERSION     DAVKZB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.146"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            104..415
                     /locus_tag="VXA80_001881"
     CDS             104..415
                     /locus_tag="VXA80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354321.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            419..1135
                     /locus_tag="VXA80_001882"
     CDS             419..1135
                     /locus_tag="VXA80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0354322.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEISSLMNEWLAYFL"
     gene            complement(1265..2080)
                     /locus_tag="VXA80_001883"
     CDS             complement(1265..2080)
                     /locus_tag="VXA80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV0354323.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYSDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2077..2982)
                     /locus_tag="VXA80_001884"
     CDS             complement(2077..2982)
                     /locus_tag="VXA80_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV0354324.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLSYSLLIGVIL"
BASE COUNT         1090 a          539 c          672 g          913 t
ORIGIN      
        1 tgctaaaatc ttatcagtga gaagatttct taaagtttat tctttgtagt ttcatctgta
       61 ttagtatata atttaactta gagaaaatag gaggactaat cgaatggaat taaaagattt
      121 tacagaaaaa gaacaggaaa tgattaagaa aaggcttaca atgtctaata ttagtgacaa
      181 agaaactact gagaagattc tggcgctagt accacaagac ttgattaagc gaatcccgtt
      241 ttttgtcaga aaacatgcta caacacgtac gattaaacgc atttcaattg aacaccctga
      301 actctacgct gtagctcaaa caagtggtga gattccagaa aaagaacgcg aagaattgcg
      361 tcagattatc acaactatct ttgaacaaaa gatgaataag catagtatta agtagagaat
      421 gaaaaaatat tttattagcg gtttgggaag caatgcctat catagcaagg atttttttca
      481 agaactagat tcgcaggtct attttctaaa tccatatgaa aagcatcttc gagatgaaac
      541 agaattgaaa tcatggttta aaaatgagat tgtagaggaa gaatctatct gtctgatagg
      601 tcattctctt ggaggagatt tagctcgtta tttcgcatcg gaatttgaag aagtaaagaa
      661 actgattctt ttggatggtg gctatctaga tttagataag attttacctt tggatacaga
      721 gttagaggaa actaaaaatt atatcaaatc tcaaattgtt ttggacttag atgttcttac
      781 ttctaaagaa aaatctgaag caaagcattg gtcagaaaat atggagaaag ctgtaagaca
      841 gtcctatcac tggaatgttg agtataatag atatgagttg gctataaatt atgaaaatat
      901 agaagctata ctccgcctac ggagaaaaat acaagctttt aagagagaag tgggagatac
      961 cttgtttatt agtcctcgct atcctaatgg agctacatgg agagaggaag ccctaaaaga
     1021 attgccagac tattttgata ctattttttt agaaaacttt ggccatgagc tttatactca
     1081 agcacctaaa gaaatctcta gtctaatgaa tgagtggctc gcttattttc tatgagctac
     1141 ttgtattgct ggaaatggta taagatgaaa atctacaagt cactaatttt tatataacaa
     1201 aaaaacgaac aggctctttt tctgttattc agaagagaac ttgttcgttt ttatcttgat
     1261 tttcttaaaa agctctatat acatagggat tttctggttt atcgactttg gtaaagctat
     1321 cgatttccaa ggtcgggaga ttttgtttga gttctttatg gtagtgattg accagttttc
     1381 ctttggctgt tatccaagtg ttgttctcat attgacgggt atttcccttg gtcagcaacc
     1441 catagacacc agaatctgcg atgcagtgaa taatgccgaa gcggaacaga aattggctat
     1501 tggcatgact ggggtcatta tagacaaagc ctgtgaactg gattgtctta ccctcaaatt
     1561 catctgaata gtcgtagata gcctccatga cttccatata gttttcatta gtgatctgaa
     1621 tactatcttg ggataagtat ttatccgccg ccgttcgcat ttccttttca taggctgatt
     1681 ttgaaaaata agaactggta tctggtttca aatattggct tgtcgtccct tcgcttgtct
     1741 gaatggctag atccgttcct tccgataggg ggaaatgata acctttagca gaaacggtct
     1801 gagaatccaa gctaacagtt gggaaagtta agccgatgac aagtggaata gccagaagag
     1861 aaatactcgt catcttggcc aatcggctgt ttagatgact gtgggttttg acttgcttca
     1921 tccagatata caattgaaca attgccaaga taaaggaaag caccatggag atataggcca
     1981 gataggaata gtgcatgttg atgtactggt ttagtttgcc cgacagatgg aggtaaatag
     2041 tcagttcaaa atagccagct aaaactaaaa atcgaatcat aggatgactc caatcaagag
     2101 agaataggat aaaacgacca gagtcacaat cgtaatgaag tgactaataa atcgtgcttt
     2161 caagtaattt ttcatcatga gaatattttt gatatccagc attgggccaa tgacgagaaa
     2221 ggccagaact ggagccaaac cgaaactcga gagtagagaa gcacctataa aggcatccgc
     2281 ctcactacag agcgaaagaa gaaaggccaa aagcatcaag agcaggatgg caaaaagagg
     2341 ggtcgcactg atagaggtca gaatccgagt cggaacgtag acctgtatta tagaagcaaa
     2401 gaggcagcca aataccaaat aacgccccgt atcaaaaaat tcatcaatgg cctgcacaaa
     2461 gacttgaaaa actttttttg cagaactcaa gtaagaaaaa tcatgctcat gacaagccag
     2521 acgattttct ttctgaatcg gttcttgcca gaaaaatcct agaaaaattc ctagtattac
     2581 agccacaaga atggaaccca gagctcgtaa tagggcgaca tggaaggagt tgccaaaggc
     2641 agaataggtc gcaaaaagaa caatgggatt gataacaggt gctgtcacaa gaaaaggaac
     2701 ggccgtgtaa cttggaacct ttttttccag aaaacgattg atgatgggga cgattccaca
     2761 ttcacaagaa gggaaaaaca taccgacgaa ggtcccaaaa aagattctcc cccaacgatt
     2821 tcgagggaga aaatgataaa ccttgtcagg tgtgatataa acttcaatca gccctgagac
     2881 aatacttcct atcagaacga agggaagggc ttcaatgata atggagagaa aaatggctcc
     2941 agtttgaagt acactagatg ggagttgttg aaaagcagtc atctagtttt tcttttctgc
     3001 tttggctttt ttctctttgt catcagggaa agtgacttgt ttcaaatcag ggagtttagc
     3061 aaactcttca aacatcttgt ccaagtcgtc tcgttttgta aatttgacag ccataggtgt
     3121 acctcgattc tagattctac ctctattata ctattatttt gcggaaaagg gaaaagttaa
     3181 aatttcaaat tgagtagcta agagataata tagc
//
LOCUS       DAVKZB010000147         3091 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKZB010000147 DAVKZB010000000
VERSION     DAVKZB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.147"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(206..934)
                     /locus_tag="VXA80_001885"
                     /pseudo
     CDS             complement(206..934)
                     /locus_tag="VXA80_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1180..1491)
                     /locus_tag="VXA80_001886"
     CDS             complement(1180..1491)
                     /locus_tag="VXA80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354325.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1506..2792)
                     /locus_tag="VXA80_001887"
     CDS             complement(1506..2792)
                     /locus_tag="VXA80_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0354326.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATLRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          920 a          674 c          540 g          957 t
ORIGIN      
        1 tgcttattaa tttttttcaa aatatctaag acatcgcttc tttgtttaat ttgataggct
       61 ttgataccta agctccgact ttatcactta aaaaataaaa aaggacagct aatctcttgc
      121 gcatcaaggg atttcgccat tctttaatct cgtttaagtg ttgtatatgg agctttgcac
      181 aattttttaa aactaaacaa ctacattaag cacgaactgt gacgctggtt ccatcttcct
      241 tataaagatt gataagcccc tctttaagag ctcgaaccat gtctcctgat tgaacaggtt
      301 gtgggacttt aatagtcaat agttccattg gatttggagc gcggtcgatt ttattgcctt
      361 tggcatcatg caaatcttca atataggttt caaaatgacg gaaacctgga ccataaaact
      421 caacttggtc cccttcgtta atgacatttc gttgacgaat agttgctgtt tgtgccgcat
      481 catcataaga aaccacttca gcgacaaact tgtactcagg aattttacga cgagcaccaa
      541 acaactgctc attttcagat ggtgtaccat agtaaaatcc tgtagccagt tcacgttggg
      601 caaccttcca catctcgtcc accaagtctt gtttgatagc ttcaaacttt tcaggacttt
      661 caagataggc atccacagcc gccttgtagc agttggttac tgttaataca tagtgaatag
      721 actccatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata tctggaatgt
      781 ggtcaatcat agacatgtca acggctgaca ttgaaaattc ttctggaatc tcaccctgca
      841 aactcttacg ttctttccca aatggcatat cgtaaaggtc gtacttccaa cggcatgact
      901 gagaacatcc accacggttg gcatcacgca tactctagac ggattgtttt gacaattcta
      961 atcttttaga ctttgcataa taactgttaa acagttgaaa attaacccag ttttaacaag
     1021 tagtcaaaat accagataaa ggaaaaaagt ctttaaaatc ttaaaaacgc atagtatcag
     1081 gtattgaaca atcttgatat tatgcgtttt attgtgggaa gatttacttc attttctctt
     1141 ggaattgagt tttctcctac ttcaaaacta gatgatttgc tatttaaaat agcgttcctt
     1201 atgattttgg caacctggat taaagcgaga aaaacaaaag gggcaactta aactttcttt
     1261 atattgctta tatgttagta gttttagaca aacaccacat aaaataggct tatcctgtat
     1321 caaagataag ggatacggct caaaagggtg attttctaat gaatcatgac accgataaca
     1381 agcataatac tttttacaat catagcattg taaagcaata atatccagct tgctatggta
     1441 atgaatgcat cttgtttgct tatccactaa gaaaccctgt gcctctatca tttccttttc
     1501 ctttcttaag ctcgaactgt gacgctggtt ccatcttcct tataaaaatt gatgagtcct
     1561 tcttttaatg cacgaaccat gtctcctgat tgaacgggtt gtgggacttt aatagtcaat
     1621 agttccattg gatttggagc gcggtcgatt ttattgcctt tggcatcatg caaatcttca
     1681 atataggttt caaaatgacg gaaacctgga ccataaaact caacttggtc cccttcgtta
     1741 atgacgtttc gttgacgaag ggttgctgtt tgtaccgcat catcataaga aaccacttca
     1801 gcgacaaact tgtactcagg aattttacgg cgagcaccaa acaactgctc attttcagat
     1861 ggtataccgt agtagaaacc tgttgccaat tcacgttggg caaccttcca catctcgtcc
     1921 accaaatctt gtttgatagc ttcaaacttt tcaggacttt caagataggc atccacagcc
     1981 gccttgtagc agttggttac tgttgatacg tagtgaatag acttcatacg tccttcgatt
     2041 tttagactgt ccacaccatt ttcaatcata tctggaatgt ggtcaatcat agacatgtca
     2101 acggctgaca ttgaaaattc ttctggaatc tcaccctgca aactcttacg ttctttccca
     2161 aatggcatat cgtaaaggtc gtatttccaa cggcatgact gagaacatcc accacggttg
     2221 gcatcacgca tactcatgtg gtttgaaaga gtacaacgtc cagagtatga aatacacata
     2281 gctccatgga caaaggcttc aatttcaaca tctgtacgtt tgcggatctc agctaattct
     2341 tccattgaaa cctcacgcgc taaaacgaca cgagtcaagc ctagctcttt ccagaactca
     2401 agggtttcat agttagtggc actggcttgg gtagaaaggt ggatttcaag gcctggtgct
     2461 tcagtcgctg caatcataat caaggctggg tcagatacga taactgctgc aatcccgata
     2521 tcacgcagtt tacggaacca ctcaccagca ccagcttcat ttccttcgtg cataaccata
     2581 ttagccgcta catagacctt ggcaccatac ttggccgcaa actgcacgcc ttcttccatc
     2641 tgttcgaaag taaagtttcc cgcacggcta cgaagaccat aggcctgacc accgataaag
     2701 acagcatctg ctccatactg aacagctacc tttagcttct ctaaagtccc tgcaggtgat
     2761 aaaacctcag gacgttttaa tgtttttgtc atgatttctc ctatttgcaa tataaaagtt
     2821 tgacattttt ccttctcatt ttatagtaaa taaaggataa aatcaagcct agacggattg
     2881 ttttgacaat tctaatcttt tagactttgc ataataactg ttaaacagtc gaaaattatc
     2941 ccagttttaa caagtagtca aaaagtctag aaaagtaaaa accagtatcc caagatactg
     3001 actcgtaaaa atacataaat tagtccttag atgactactc ccactctgtt tctcctataa
     3061 tggaaaagga ttgatacagt ctagaatatc g
//
LOCUS       DAVKZB010000148         3086 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKZB010000148 DAVKZB010000000
VERSION     DAVKZB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.148"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(241..561)
                     /locus_tag="VXA80_001888"
     CDS             complement(241..561)
                     /locus_tag="VXA80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0354327.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            1318..1698
                     /locus_tag="VXA80_001889"
     CDS             1318..1698
                     /locus_tag="VXA80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEV0354328.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGGVGCNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            complement(2115..2963)
                     /locus_tag="VXA80_001890"
     CDS             complement(2115..2963)
                     /locus_tag="VXA80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354329.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVETHY"
BASE COUNT          942 a          433 c          617 g         1094 t
ORIGIN      
        1 tattgatgat gtctaaaatc tggatattag ggtctttaat gtctagtaat tttgtgataa
       61 aatgtaattg ttccatatga ttctttctaa tgagttgttt tgtcgctttt cattataggt
      121 catatgggac tttttttcta cacaaaatag gctccataat atccataggg gggttaccca
      181 ctacaaatat tatagagcca caaataatat gtaaagagtc gaggaaatcc ctcggctttt
      241 ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag acagcataga
      301 agtttcctcg gtcagctcta ggagtgagaa ccctttttgg ataagtttcg catcatccct
      361 acaaatccca atgcggacat agcagatagc cagcctatcg tagcttttct tattcttggc
      421 gtcctccagt aaagtgtagc agatttgctt acacatgttt ttatcttgat tgtatagata
      481 gagtgtggaa aggttgagca ggattgctat tcgcaagtca tataaatgtt gatagttttt
      541 atagacttct aagcgttgca agattttctc ggattattga caaataaacg gacattctga
      601 tagacgattg ccctagcggc ttagtgtaaa ctttacaaat ttggcttgta atttttttat
      661 aaaattcact ttagttactt gacagttatt atattttcgc tcataatata gataaaagga
      721 tgggatagga gtttaaaaat ttgaaagcgc tatccataat cataacgcat aattttctcc
      781 ttttcgtgaa aaaagttaac gtaattttga aatgagctta ataagaggta ctatgtaggt
      841 agaaaataaa gataggagaa aaagtatgaa taaaaaaata ttttgtattc tagtatgtat
      901 tatatcattg attagtctag ctatagtctt tccatggggc tggtcgatat aatgtaaggg
      961 agcttgagtt gttagtggag gataaaatgt taagagtaat agtagttatt caatctttag
     1021 taataatact gcttgctgtt gctttattat tatctcggaa aaactttgat tcgttaaatg
     1081 ttgttcttat aatgatgatc gtatcacaat tgttgttatt agtatatttg ataaaatcag
     1141 ggaatcagtg attagctgtt atttgaatta agtaaactgt taagcttcaa gcagtaactg
     1201 gttttaaata tttaaaataa gataaaggaa ggaagtctaa tttaggtaac taaacaaagc
     1261 ctgcgatcac ttgttaggaa accatcctaa ccatgcttaa taaaaaggag aaaacaaatg
     1321 gaacttgtat taccaaataa ttatgttgtg attgatgaag aagagatgat gtaccttgat
     1381 gggggtggtg ttggttgtaa ctggtggaat agtagaggta gttttgcaac agttctggat
     1441 gtaggtttgg ccatctatag tggtggtgca acaatttatt ctgcttatgc gataaaaaaa
     1501 gctatctcag ctaatagagg ggctattacg agaacattac gtagtttaat aattaaacat
     1561 gtaggtagtg cagctggcca tttagtcaat actgcactaa acgttgcact aactgttact
     1621 ggattttcac taggtggagc aatcgcatat ggggctgatt gggctgacgg tagcttagat
     1681 ggttatattt ttgcttaaaa tgaaacattt ttttgcagga attggtgaaa taaactttgt
     1741 aagttattta ttgtatatat gtgtaggaat agcccctttt tttcacgtat atataatagg
     1801 ttctgaaatg aattttgtga aaatagtgtt atctattttg ggaattatat ttgtttgtat
     1861 tttaactatt gctcgaattt ataggacttt tttctataaa gaatagtttg gacttgaaat
     1921 gtatttaaaa acaagaggtt ggtagattat cagcctcttt cttgtcgttg aaaaagtgag
     1981 atatattgac ttttgaaaaa aatgtcataa ttgagttggg ataaacgtaa aagtcatact
     2041 ttctcgttaa atcgttcggc gattctatga aaaggtcgag gagctccctc gacctttttt
     2101 tataatttct ttggttaata atgggtctct acttcttttt tgagatgaga cagcatagaa
     2161 gtttcctcgg tcagctccag aagggagaac cctttttgga taagtttaga atcgtctgta
     2221 caaatcccaa tgcggacata gcagatagcc agcctatcgt agcttttctt attcttggcg
     2281 tcctctagta aagtgtagca gatttgctta cacatgtttt tatcttgatt gtatagatag
     2341 agtgtggaaa ggttgagcag gattgctatt cgcaagtcat ataaatgttg atagttttta
     2401 tagacttcta agcgttgcaa gatttttcca gtgatgagat ggaggtattc aatgggaaaa
     2461 ctgaaaagaa tggtattgag gatttttaga tcactctcat accatgtatc ttgtttttca
     2521 atttttctcc aaagtttttt gacaacctgt tctgcgtgat ctgatagttc ccctgccccg
     2581 tgttgacgga tataaatgac aacttcaagc atatctctga tttcttctat agggagatcg
     2641 tggtgggtct tgaggtagtc ttggcatttt tgaaataaat tgacaagatc gctggtcccg
     2701 ataatggaac gcatattgag ataggtttgc ataatctctg tgcgttgact tggttgataa
     2761 agttgacaga tatattcaaa ctcttcaaaa ctcatattga tttgacggag aagaaattcc
     2821 atattttcat attttggggt tgtcttgcca ctttcgattt ttgataagct ggttcttgag
     2881 agaacacccc cacagacctc ttcttgggtc agcccctttg actcacgtat ttctttatag
     2941 acctttccga aatcataacg catgattttc tcctttctgt gaaaaaagtt aacaaataat
     3001 aaaatcttgt taaaaatatt gtatcatagc aatgtaaaaa gtaaaaaaga aagtcgaaat
     3061 aaatttgtga aaaaagttaa cgtaat
//
LOCUS       DAVKZB010000149         3033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKZB010000149 DAVKZB010000000
VERSION     DAVKZB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.149"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..160
                     /locus_tag="VXA80_001891"
     CDS             <1..160
                     /locus_tag="VXA80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0354330.1"
                     /translation="FAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTLITGKN
                     GTGKSTLL"
     gene            complement(526..1836)
                     /locus_tag="VXA80_001892"
     CDS             complement(526..1836)
                     /locus_tag="VXA80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV0354331.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2073..2945
                     /locus_tag="VXA80_001893"
     CDS             2073..2945
                     /locus_tag="VXA80_001893"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV0354332.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          926 a          510 c          694 g          903 t
ORIGIN      
        1 ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa aatatttcgt atagggtgga
       61 tcatcgtata ttgtttgata atatttcttt tgatacttcg agttcaggcg tgacattaat
      121 tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa ctagaatagt
      181 acacatctac ttctaaaata ttgttagaaa tcgatttgac tatcctgatc tatttgtcct
      241 gttcttattt catttcacta catctcaaat tgagtatgac gaagtgcgct cccatgtcct
      301 gggaacgcac tttcttcata tttttcatat tcttgaatcc atcgataaag actattggga
      361 tgaattttta aagttgaact aatcattttt acaggatgag gtttacagca gagagtttga
      421 aggctttatc aaaggttttt cttggcataa tgacttttcc tcgtttccac ttaattttgt
      481 gtctacttta ttataccaag tccaccctta agttagataa taaatctaac ttaaggaagc
      541 tagaaggatg agaatccagg tggtcaagag tcccaaactt aagctgatgg ggacacccag
      601 aataatttgc tttttgaagg caaggccacg ttcctctata ttgggaagtg agagttgaat
      661 gagagaacca gctgatgaaa agggtgagat attagtagat agagcgccaa taacggtggc
      721 tgttgtgagt aagtgaatat caatctgagg attttgagca ctgatgatag caatgatggg
      781 aaagagggct ggagctacaa cggatagggt ggaactaaag agtgacatca ctccggctat
      841 cacacaaaag aacagaggta accagaaatg aggaatggat gttgtcatga ggtgccctat
      901 cagtgtgact aaacctgact tgaccgctag agacattagt aagctcatgc cgcagagcat
      961 gataattgta gcccagggaa ctttagccaa aatggcttct tgcttcccta atttgagcct
     1021 taaggcgagg cagaccatga gtattgagac aaagccaata tcaaatgttt tttgataagt
     1081 agctatccag gcgatgtttg ggaaaatgag atgcaacaag ggaaaaagcc aaaccaaaac
     1141 catgctgctg atcatgagca aggtggtttg tctttgaacc ttgctgagga gtggtggttg
     1201 gtcaatagtc aaggatgagt ttgttcttcc cttactatag tgactgtaac aggataataa
     1261 aagcaagacg atgagtgggt agataatgct gacgataaag atatgattgc caagtgaaaa
     1321 agcttgctct tcccatccca tttgcttaaa caggccttga aagacaatgc ctgagctact
     1381 ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg gcttgagctc caatcaaagg
     1441 gtgtttgtcc gctttttgac agagggtaat cgctagagga cagcaaacgg ccatagtagt
     1501 gaaaaatcca gcacctaaag cagacaaaag ggttgccatc aggtataaaa tcatgtagag
     1561 ggcgttaggg taggtgcgtg tgcggtagag aatgtgttga gccaaaacat caagagtacc
     1621 gttagttatt gcaaagttat aaaagagaga gacgctaaaa atggtaaaaa agagtgaggt
     1681 tggccaaaaa tgaagaagtt ctttggggct taatcccatg agagtggttg cgatgaggta
     1741 agaaaaagca atagccagca ggccaatatt gattttggtg cggtaaccaa ttccaatggc
     1801 tagagcaatg gcgctaatca ttattaaatg aatcattgaa ttgtcctttc agttagaata
     1861 tggaaatagg ttggcaattt tggcgctcaa aagaccacct gtttcctatt ggtacttgac
     1921 tattatacac aaaaaaaata caattgtctt gacaattgta ttgacacttg ttagaattaa
     1981 aaatgaggta ttcaggcttg tgatttccta tggaagttaa tagtgattgc ctctagtgct
     2041 tacaagtgat attaaaaata gaggacttag tgatgtcaat catttcaact gatttaaccc
     2101 cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat tccgaagatt
     2161 acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt tttgccaagg
     2221 aagctggtgt tttagcggga ctaaccgttt ttcaaagggt ttttacccta tttgatgccg
     2281 aggtgacctt ccagaatcct catcaattta aggatgggga tcgtttgact agtggcgatt
     2341 tggttttaga aatcataggc tcggtgagaa gtctcttaac atgtgaacgc gttgccttga
     2401 attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta gaagccttag
     2461 gcgatgattg cattaaggta tttgatactc gaaaaactac tcctaattta cgtctttttg
     2521 agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta tcagatgcta
     2581 tcatgctaaa agacaatcac attgcggcag taggtagtgt tcaaaaggca attgctcaag
     2641 cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagc cttgctgctg
     2701 ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt ggataatatg tcattggaac
     2761 agattgaaca ggccattacc ctaattgcag gacgttctcg gattgaatgt tctggaaata
     2821 ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc tccagtggta
     2881 gtttaaccca tagtgctaag agtcttgatt tttccatgaa gggtttaacc taccttgatg
     2941 tctaagttgt aaaataaact aactttttaa aggatgtctt tcctctagaa cgagttttat
     3001 gtcagatagt ttaaacgcct cttcaaatat agt
//
LOCUS       DAVKZB010000150         3021 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKZB010000150 DAVKZB010000000
VERSION     DAVKZB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.150"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..392
                     /locus_tag="VXA80_001894"
     CDS             <1..392
                     /locus_tag="VXA80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV0354333.1"
                     /translation="STDIERIGDRIVEILKNLQILQNNEILKKIISKIKILHEVIGLH
                     MNRAISCYREEQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNVSEVISALDI
                     IHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            405..1880
                     /gene="galT"
                     /locus_tag="VXA80_001895"
     CDS             405..1880
                     /gene="galT"
                     /locus_tag="VXA80_001895"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0354334.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1893..2873
                     /gene="galE"
                     /locus_tag="VXA80_001896"
     CDS             1893..2873
                     /gene="galE"
                     /locus_tag="VXA80_001896"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0354335.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
BASE COUNT         1099 a          397 c          620 g          905 t
ORIGIN      
        1 tatcaactga cattgaacgt attggggata gaattgttga aattttaaaa aatctacaga
       61 ttcttcaaaa taatgaaatt ttgaagaaaa ttatttcaaa aataaaaata cttcatgaag
      121 ttatcggact gcatatgaat agggctatat cttgttatag agaggaacaa tctggatgtt
      181 tagatatggt tgttatacaa aaacagaatg agatagaaga gcttagtata aatatggaaa
      241 agaaaataat gaattacata tttgaagatg atgggaacgt ttctgaagta attagtgctt
      301 tagatattat tcatcacctt gataaaattg ctcatactac gcaagcaatt tataaatgga
      361 taatgtatag aaaatatggg aacataaatt agaaagaaaa atatatgaaa acaattattg
      421 ataattttgt tgatagagta attgaatacg gaatgtataa tgaaattgac aaaatatatg
      481 tgaaaaatag agtactagcg ttaataggtg aggagggaat tgatcgcatt tccgatgaaa
      541 atgacctgaa acaaataaaa gattacttag tggaaatcgc cttaaaaaac ggtaaaatta
      601 aagatttaat cgaggaaaag gaatgtttag gagcagagtt aatgaatttc atagttcctt
      661 tacctagtcg attaaatgat attttttgga gttcatatga tatatcacct caagaagcag
      721 ttgaagagtt ttataagtta agtaaagata gtgattatat taaaacttct gctattgcta
      781 agaatattga gtttcgagca tcaactaaat acggagagct agagattacg ataaatttgt
      841 caaaacccga aaaagatcca aaaacaattg ctgctgaaaa attagtaaaa gcaactaatt
      901 atcccaaatg tttactatgt atggaaaatg aaggatatca ggggcgaata aattatccag
      961 cacgttcgaa tcatagaatt attcggttga aacttggtga cgaagtatgg ggattccagt
     1021 attctcccta ctcgtacttt aatgagcatg cgatattttt gaatagtcaa catgtaccaa
     1081 tggctattac ctctaaaaca tttgaacaat tattggagat tgttgatatt ttgccaggat
     1141 attttgctgg ttcaaattct gatcttccta tatcaggagg ctccattctt agtcataacc
     1201 actaccaggg agggaaacat attttcccaa tggaaaaagc aaaatttgag agtgaatttt
     1261 gttttaaaga ctttgaggat gttaatgctg gtattgtaaa atggccaatg tcagtaatta
     1321 ggttacaaag tgaaaataaa aatcgcttgt tagatttagc tactaaaatt ctaaataagt
     1381 ggagagaata ttcagattta gaagtagaca tcattgctat gactgaagat gtcccacacc
     1441 atacggtgac tcctattgct cgtaaagtgg acgggagata cgaattagat attgttctaa
     1501 gagataatca tactactgaa caatacccag atggtgtttt ccatcctcat caagatgtac
     1561 aacatattaa aaaggaaaat atcgggttaa tcgaagtgat gggacttgcg atattaccac
     1621 ctcgtttgaa accagagtta gaagaagttg gaaaatatct tttaggagag gataatgcaa
     1681 ttgcggatta tcatttagaa tgggccgacc aattgaaaga aaaatatcct cgcattaata
     1741 aagaagaggt taatagcgta gttcaacacg aagcaggaca agtgtttgcg agagttctgg
     1801 aagatgcagg agtttataag aatacaccgt ctgggcatga agcgtttatg agatttgtta
     1861 agtcggttgg aattaattag gaggaaaccc agatggcaat attggtaaca ggcggagctg
     1921 gttatattgg tagccatacc gtagtagaat tactaaattt aggaaaggaa gttatcattg
     1981 tcgacaacct ttcgaactct agcaaattgg tgctagaccg tattgaagca attacaggaa
     2041 tacgtcccgt gttttacgaa ttagatgttt gtgataaaca agcattgaga aaggtatttg
     2101 aacaagaatc gattgatgct gcaattcatt ttgcaggtta taaagctgtc ggagaatccg
     2161 tgcaaaagcc tgtgatgtac tacaaaaata atattatgag tacattggca cttgttgaag
     2221 tgatgtcaga atttaatgtt aaaaagattg tcttttcttc aagtgcgact gtatatggaa
     2281 ttaacaatca gtcacctcta attgagacga tgcaaacaag tgcgacaaat ccttatgggt
     2341 atacgaaagt gatgcttgag caaattttaa aagatgttca tgtggcagat tcagaatgga
     2401 gtattgcgtt gcttcgttat ttcaatccaa ttggtgctca tgagtctggt ttgattgggg
     2461 aagatccctc aggaattcct aacaacttga tgccttatat tgcacaagta gcggtaggta
     2521 agctatcaga gctaagtgtg ttcggcaatg attatgatac gcttgatggt actggtgtgc
     2581 gtgattatat ccatgtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat
     2641 ctgaaaaaac agatgtttat atctataacc taggctcggg agaaggcact agtgtattac
     2701 aacttgtaaa tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa
     2761 gacgttcagg agacgttgca acttgttatg caaatgcaga caaagcatat aaggaattaa
     2821 attggaggac aacaaaatcg attgaagaca tgtgtagaga tacatggaat tagcaatcaa
     2881 aaaatcccaa tggctataat tgatgatact gtctatatga gtgctgtatt ttggaaaaat
     2941 tacttaacct tttaatgaaa tgcatattta atgaaagata aattaaaaaa aatgtttgac
     3001 agcgcttgac aaaggaaacg g
//
LOCUS       DAVKZB010000151         2994 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKZB010000151 DAVKZB010000000
VERSION     DAVKZB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.151"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(171..1094)
                     /locus_tag="VXA80_001897"
     CDS             complement(171..1094)
                     /locus_tag="VXA80_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0354336.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(1108..2037)
                     /locus_tag="VXA80_001898"
     CDS             complement(1108..2037)
                     /locus_tag="VXA80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0354337.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(2356..2577)
                     /locus_tag="VXA80_001899"
     CDS             complement(2356..2577)
                     /locus_tag="VXA80_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354338.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(2531..2791)
                     /locus_tag="VXA80_001900"
     CDS             complement(2531..2791)
                     /locus_tag="VXA80_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354339.1"
                     /translation="MTGVSNPTTDSAPLVLAEAKKAFADDSLIEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT          959 a          591 c          592 g          852 t
ORIGIN      
        1 agccgcaggc tgctcaaagc actgctttga ggttgtagat aagactgacg aagtcagtta
       61 catatatcta cggcaaggcg acgttgacgc ggtttgaatt tgattttcga agagtattaa
      121 cttcatacaa gggaagttgg gaactgagaa atgttctttt tcaataagca ctattctttc
      181 acaccaccga tagtcaaacc ttttacaaag tagcgttgga agaatggata taaaatcgcg
      241 attggaaggg ttgcaaccac aaccatggcc atacgacctg tttctttcgg tagagcaact
      301 cccagttgac cagtcaagcc gaccgctttg gcaatgtagt ccatattttg ttggatttgc
      361 atgagcaaat attgcaatgg atacaagtta tcactcttga tgtaaagaag ggcgttgaac
      421 cagtcattcc agaaaccaag agctgtcaaa agcgtgatgg ttgcgatacc tggtagtgac
      481 aatggcaaac agatttggaa gaaaatccgg gcctcactgg caccatcgat acgagccgat
      541 tcgagaatgg cttctggaat ggtcttcttg aagaaggaac gcatcaagat gatattgaat
      601 ggtgagagaa gcattggaac aatcaaggcc caaacagtgt caccaagctg aagtacacgg
      661 gtcaccatga tataacctgg taccaaacca gcgttgaaca acatactgag aaggacgaag
      721 atggtaaaaa atctgcgata cttaaaggtt gtccgtgaaa tagcgtaggc ataggttgtt
      781 gtgataaaga catttgtcaa tgtcccaact acggttacaa agacagagat gaagagggct
      841 tgtaggattt tatccttaaa ctgtgccaaa aactcaaaac cgtctaagcc aaattgggat
      901 gggaagaagc tatagccgta ttggaggagg cttttctcgt ctgtcactga aataatgata
      961 acgaatacaa aaggtaggat acaagagagg gcaatcaaac ccgaaatgat actgaagaag
     1021 atatctgctt tcttactgaa ggagtgaatg ccgacattat caattttttc ttttttaatt
     1081 ttcttttttg ccatattctc ctcctttcta gaacaaagct gagtttggat cgactcgtct
     1141 tgcaagcaag tttgatagga taaccagaat caaaccaaca acggattggt aaagaccggc
     1201 tgctgcagcc ataccaatat ctgctgtctg agtcaaacca ttaaagacat atacgtccaa
     1261 aacgttggtt acattgtaaa gctgaccagc attgtgtggg atttgataga agagaccgaa
     1321 gtctgcgcgg aagatatttc cgactgcaag gatggtcaat acagttacaa gcggagtcaa
     1381 ctgaggaatg gttacgttgc gaatacgttg ccacttgcta gctccgtcca ctgtcgctgc
     1441 ttcgtagtag gttggatcaa ttcccatgat cgtcgcatag tacatgacac tgctatatcc
     1501 aaagcctttc caaataccta ggaaaagtag gagatagggc cagatgccca ggtcagcgta
     1561 gaaattgact tctttgagac caagactttc caatagatga ttgaacaccc ctttatcaat
     1621 atttaggaag gcatctgtaa agaaactgat gataacccaa gacaagaagt aagggaacaa
     1681 catagaagtt tggaaaatct tcaccattct cttagaacgg agctcgctga ggataatggc
     1741 aatccctaca gatacaacta aacctagaaa gataaagcca agattgtaga ggacagtatt
     1801 tcgtgtgata ataaaggcgt ctcttgaact aaataagaat ctaaaattat cgagtccgac
     1861 ccatttacta tttatgatac tatctatgaa accattactg gtcatgtggt agtctttgaa
     1921 ggcaaccacg ttcccaaata ctggaatgta aaagaataga atcaaccaga gtgcccctgg
     1981 caaaaccatc aagagaaaga tccagttgtc tctcaatgtt tttgaaaact ttttcataat
     2041 ttcctccctt tttattttga tatccatcta aaaattcttt tttagacttt tgataacgat
     2101 tacattatta gtatactcct atttgcaggt taggttaaac tcctaattat agaaaaaact
     2161 ccacaaatta tgtagcagat ttaaaacttt atcaccacta tcaaacaaat gtcctaaatc
     2221 aattgtttat tttatctcta ttagcccagt gatggcgtca ctctgttata agcatccaac
     2281 aacggggtat actgaaaaat ctccagacta gggaactcag cgatagttcc taatctggag
     2341 atttttaata tgttattagg cgtttgcttt caacttagca ataacctctt taagattatc
     2401 aatcaactct gctgcagtat gctcagagcc tttttcatct gccaagaaca aaactgcttt
     2461 ttgaagttct tcttgagagt tttcaaggac atccttatct actgtttcaa ggtttgagtc
     2521 tttaagaagt ttacttaatt ccttggctaa tttcttgagt ttgatttgca gactcatctt
     2581 ctcctgctgt ttctttgccc gctgtttgtc ctccatcctt agttgctgac tggctttcct
     2641 taatggactc tagggaagca atggcatctt tgactgtttg caagatatca cgtaaacctt
     2701 gctctatcaa actatcatct gcaaaagctt tcttagcctc tgccaaaacc agaggtgctg
     2761 aatctgtggt aggattcgat acacctgtca atgatctcaa aagattttct aaggtttgag
     2821 tctgcttact aatactagac tcaaatcaaa aagcattata taacagtgat atgaaatcaa
     2881 ctaaagaaga aatccaaacc atcaaaacac ttttaaaaga ctctcataca gctaaatatc
     2941 ataaacgcct tcaaatcgtt ctatagtaaa atgaaacaag aacaggacaa atcg
//
LOCUS       DAVKZB010000152         2973 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKZB010000152 DAVKZB010000000
VERSION     DAVKZB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.152"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(18..1247)
                     /locus_tag="VXA80_001901"
     CDS             complement(18..1247)
                     /locus_tag="VXA80_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0354340.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(1403..1705)
                     /locus_tag="VXA80_001902"
                     /pseudo
     CDS             complement(1403..1705)
                     /locus_tag="VXA80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1810..2088)
                     /locus_tag="VXA80_001903"
     CDS             complement(1810..2088)
                     /locus_tag="VXA80_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEV0354341.1"
                     /translation="MEKVASLGVPMCKPISIELCDDEVHSLHEWIDGRDAIDSILTYS
                     ENQQYTYGVEAGKILRKIHTIPATEVCEDWEIFFYLKIDDKISNEMIW"
     gene            complement(2340..2927)
                     /locus_tag="VXA80_001904"
     CDS             complement(2340..2927)
                     /locus_tag="VXA80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV0354342.1"
                     /translation="MGGNPPIKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
BASE COUNT          969 a          610 c          454 g          940 t
ORIGIN      
        1 actatccttt ctttatccta cttcaacaga aggttcattg gttgattttc caattctcgg
       61 tacaacttag cgcgtttttc actggcatca atatttaaga caaaggctac tgccactgat
      121 aagactagaa gactatttcc accctgggat aagaaaggga aagtcactcc tgtagatgga
      181 atcaagcccg aaatccctcc gatattgaca aatacctgaa ccaacatcat ccctccgaca
      241 ccgagtgcaa ccatggcatt gaaaggattc tccgctcgga taccgaccaa gataatccgc
      301 aaaatcatga aaaacaagag agctaaaata agactggcac caacaaagcc aaattcttca
      361 atcacgatag aaaagacaaa gtctgtatga gcttctggca aataacctcg tttttcaatc
      421 gagtttccaa gacctagacc aaaccaacca ccattgacca tggcaaaata agaattagct
      481 aactggtgac ctgcatcagc acgatcggca aaaggattaa aaaaggcact aaagcgcttg
      541 gctacatagc caaatactgg aatttttgaa aaggtctcaa caccgattag gctgatagtg
      601 gtcaagacaa agacagaagc ggcagatacg agcgccagaa tggttgaaaa ccagcgataa
      661 gcgattccac taactgtata cataatcaag gaaaccaaga ctaaaatagt cgcatttcct
      721 aaatcaggga aaattcccaa acttccaatc agaactagga gaacgaatcg ccaatcatta
      781 aaagcacggg gaagccattg attttgagtc aaaacttgaa aatcataagt agctatttct
      841 tcttgctgtt tggagaatcg gtgagctaaa taccaaataa taatgatttt taagtactca
      901 gctggctgaa tagttattcc tgcaaccgaa atccaaccgt atgccccgtt tacggaaata
      961 ccaataaaac gagccaagaa caataaaagc atttctatta atataactaa aatgattagt
     1021 cgctcatttc tcaaaaaatc tagtctcaat ttataaatta aggcaatcag tatcaaacta
     1081 acaatccaaa agattccttg gtttcgaacc aactgcaagg cgctcttgcc ttcttcaatt
     1141 aaaatagcac tggtggtcga atagaccaca atcaagccca aaatagataa aagcaagtag
     1201 ggaatcaaga tggaataatt taataagtgc ctcttactaa tcttcatagt atcaccactc
     1261 tactaggata cagacaaatc tgttccaaat tccgttttat tttctagttt tgattgctat
     1321 tataccattt tttcagaaag aaaaaaactc ttaccttaca atccttcgaa ttttactgtt
     1381 tttacagaat taaggcagaa tttgaaatcg taaaaccatt ttgaataaga gttgcttcaa
     1441 cccattgact ccgacggatt aagttgcttt cgtgaatgcc aaaatcagcc gcaatttgtt
     1501 cataagtgct ctattctcgc atgtattgaa tagttaccat aaggagatcg tctaggctta
     1561 atttgctttt tcgtccacct tttgcgcgtt tacgttgata agctgttttt aacacagcta
     1621 acatctcttc aaaagtcgtg cgctgaacac ctacaaggat cttgaaacgg ctatcgctca
     1681 attgtttact tgcttcatca tccatagttt tattgtattg tatttttgtt tcgcaggaag
     1741 tctattattc ctttcgttcc aaggttttta cgcttttagc aacaagctta cacactgagc
     1801 gtttcggact taccatatca tttcgttgga gattttatca tcaattttta gataaaaaaa
     1861 gatttcccaa tcttcacaaa cttctgtagc aggaattgta tgaatctttc taagtatctt
     1921 tcctgcttct acaccatatg tgtattgttg gttttctgaa taagttaaaa tgctatctat
     1981 tgcatctctt ccgtctatcc attcgtgtaa agaatgtact tcgtcatcgc agagttcaat
     2041 gctaattggt ttacacatcg gaactccaag agaagctact ttctccatcg tatcaaattc
     2101 aattttttta taatctaact tcgacttatc agaaacacgc aagaaatatg tttactgatt
     2161 ttgaactgtc acacaatatt ttttatccat ctgaccagcc tttatttatg gctatcttac
     2221 tactaaaatc catcaagcac actccgattc actttacaac actttttgca gttttcacta
     2281 gtaacctatc cagttcgtct acacttttat ttcctagatc catttttaca ttatgaaagt
     2341 tagtcaattt tctctatgat gtttccaagt ttatccaata caagacaaga atgatcattt
     2401 agtggaatta tgggaacatt aacgagttct tcagctctat ccttatttgc tttatcattc
     2461 caggaatcat aatggacact aattaaaaac tgactaaaga gtcctaatcc attttttatc
     2521 tttatctgat gatctgaatt atcattaggt gagacataga ctttttctcc taatagtagg
     2581 gctcctgcag aaaatcctat aatttttgcc tctttattaa gcatatgatc gatataactt
     2641 ttgaactcct gattaacata agtagcaata tatttttccg tatttccacc accgatgata
     2701 attatgtcag catctagata gctatcaaga tcaatctgct cagtgtccag gagcaagtaa
     2761 tcaatgttta gattaggaaa ttgactttga aaaacttccg tgtacttttt catgtacggt
     2821 tcccaatttt ctcgaaaaac tgtaaaaatg ataattcttt taatcttggt tgataataca
     2881 attttatcaa caatactata tttctttatt gggggattcc cacccatcaa aaaaatttgt
     2941 tctttcaagt taacacactt tctaaagata cat
//
LOCUS       DAVKZB010000153         2938 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKZB010000153 DAVKZB010000000
VERSION     DAVKZB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.153"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..164)
                     /locus_tag="VXA80_001905"
     CDS             complement(<1..164)
                     /locus_tag="VXA80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354343.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKLSI
                     NHFYILLNRLG"
     gene            258..1478
                     /locus_tag="VXA80_001906"
     CDS             258..1478
                     /locus_tag="VXA80_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEV0354344.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1491..2672
                     /locus_tag="VXA80_001907"
     CDS             1491..2672
                     /locus_tag="VXA80_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0354345.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT          999 a          395 c          622 g          922 t
ORIGIN      
        1 ccaagacggt ttaacagaat ataaaaatga ttaatagata attttgtaat gccattttcg
       61 aaacgagaaa tagttgattc actaatttca ttacatgata aatctttaag gcgaaaattt
      121 tttgaaatac gaatatcctt taaaactgct ccgaaatttt ccataatgtt cctcttccat
      181 ttctgcaaaa aataaaacgc ttgtttttta tttatgatat gattatacaa aaataaaata
      241 gaaaggacaa taatatcatg aaaaaaattt tattgtcaac agttgcttta ttaagcttag
      301 tagcatctct gctagcaaat aatccagttt ctgctcaaga atcatcctct caagcaactt
      361 atagtaagtc aagtggaagt tggataaaat ctggtaaccg ttggtggtat aagcattctg
      421 atggttctta tactacaaat ggatgggaaa aaataaatgg tacctggtac tattttgata
      481 gtgaaggttg gatgaagaca ggttggatta aagaatatgg gaaatggtat tacctagatg
      541 atagtggtgc tatgaaaact ggatggtgtt tggtttctgg tagctggtat tatttaaata
      601 gtagcggtgt aatgcaaacg ggactacaga ctatcaatgg aaaacaatat tatttagctg
      661 ccggcggtgc tatgcagaca ggttggcaca atatcggaga tgatacctat ttctttgcta
      721 atagtgggga aaatcaaaat atcaatcgtc gtgctttagt tcttggtgaa acatcaacga
      781 gagcagtacc tattgcagat gttaatgcta tggaaaaggt ttttaataac caaaacttta
      841 gtgaagttgt tcgtttccca gatagaacaa aatccgagat aattgcaaaa atgcaagagc
      901 tttttgaatc ttctagcgaa ggcgatgtga actatcttta ctttacttgt catggaggaa
      961 gagatggaag aatctatatt ggtagtgatg gcctagcatt ttctggttgg gaattggcgt
     1021 ccgttttgaa acaatacaaa ggtaagtttg ttgttatgct ggattgttgt catgctggaa
     1081 caattatttc taaagataat acaggtgaag gcaatgaagg agcctctacg gaatattttg
     1141 atttagatga atttgtatca ggattttcta atatggatgg taacgaaaaa tcaggtgaga
     1201 tgattgactc taaattttta gtattatgct catcaagagg tgcagaatac tctagtggtg
     1261 gatcattaag tttagcgact aaatattggt ctttaggttc aggatggaat cctctacaga
     1321 attcacaagc atatttagca gcagaccaaa ataataatcg cagaatcaca ttaaatgaat
     1381 tgtatacata ctctcgtgaa caagttttga aacaaaattc taaccagcat attgaagttt
     1441 atcctgacaa cagtcaattt gtgttattta agaaataagg gggcaataaa atgaaagtat
     1501 caaaaaaaat tacactattt agtttgtctt ttgcaggttt tgttttattg actttacctc
     1561 aagcaggaaa ggcttttgaa cttaaagaag actgggcatt taaaggtggc attcgatacg
     1621 agaatgggaa agtcagcaaa attaataatg gatatgaagt aaatattaaa gtgttagatt
     1681 tacctagtac tagcgcaatc gaatggacag ttagattgaa tggagaaaag caaaatacta
     1741 acttcttagc ggaggaaaga actgtatcta aaactgaaga taagggacgt ttcttgcact
     1801 tttatatccc ctatggatat cgtggggata ttgtagtaga ggctaagagt ggaaacgaag
     1861 tgaagacttg gtctactaag gtagttgacg atgtttattc agattctgct aagagtggct
     1921 actttattct cgatggggaa caaatcttag aaagttcatg ggattccgta aatgagtctt
     1981 atattgcaac gcttccaact gtaacatcag gaaaaactgt tgttgcttgg cgtgaaaaag
     2041 gaactcttaa tttaattaaa ccaggaagaa ttgctcggca atataatagc agtggatcat
     2101 atgtagaact ttctccaatc tttgaaactg ctagttggct aaaatcaaac caaaactggt
     2161 actatcaaaa acaaggtcaa ttagttcaaa attcttggat caaggatcag ggaagctggt
     2221 actttatgga tgatgaaggg gttatgttta atcaaacatg gctacaccaa ggtggaagct
     2281 ggtatgcctt taagtcatca ggagccatga tttcagctga ttggttgtat gataatggtt
     2341 cttggtatta cctaaaggac tcaggttcta tggtaacagg atggcttaaa aatggaggct
     2401 cttggtatta cctaaacaag tcaggttcaa tggctacagg ttggattaaa gatagtggaa
     2461 cttggtatta tctaaagaat tcaggctcta tggcgacagg ctgggtgaaa gatagtggct
     2521 cttggtatta tctaaagaac tcaggttcaa tggcaacagg ttgggtcaaa gataatggta
     2581 agtggtacta tctcgcatca tctggtaata tgcttcgcaa tacacgcaca ccagatggct
     2641 attatgtaga tggcagtggc gcttggaaat aaatttttaa aagagaaaat ctactgagaa
     2701 ggtagatttt ctttttagtt aatgaatata gaataaagta gatcaggact ggtctatttt
     2761 attttgtaac tattaatgaa tagtcttaat gatgaagtta ctttctgaag atatttaatt
     2821 gtgttgagat ttgataaaac tatagcggat tgaaataaga tataaacaaa gagattatgg
     2881 aagtcaaatt aatttctaaa aacgttttaa aatccttagc gtactatttt agattcaa
//
LOCUS       DAVKZB010000154         2917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKZB010000154 DAVKZB010000000
VERSION     DAVKZB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.154"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(98..2356)
                     /locus_tag="VXA80_001908"
     CDS             complement(98..2356)
                     /locus_tag="VXA80_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0354346.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VXA80_001909"
     CDS             complement(2585..2815)
                     /locus_tag="VXA80_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV0354347.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          785 a          602 c          607 g          923 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata cggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggtccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt catccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgttaat tctgctagtt taaagat
//
LOCUS       DAVKZB010000155         2709 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKZB010000155 DAVKZB010000000
VERSION     DAVKZB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.155"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            36..107
                     /locus_tag="VXA80_001910"
     tRNA            36..107
                     /locus_tag="VXA80_001910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Gln,seq:ttg)
     gene            269..1471
                     /gene="rpsA"
                     /locus_tag="VXA80_001911"
     CDS             269..1471
                     /gene="rpsA"
                     /locus_tag="VXA80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV0354348.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1556..2709)
                     /locus_tag="VXA80_001912"
                     /pseudo
     CDS             complement(1556..2709)
                     /locus_tag="VXA80_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          819 a          459 c          609 g          822 t
ORIGIN      
        1 cgggggttcg aatccctctc tctccatttc attaatgggg tatagccaag cggtaaggca
       61 agggactttg actccctcat gcgttggttc gaatccagct accccagttc ttaggtaata
      121 atcaagataa aaagcaaaat atcttagggt attttatttt tataattgaa agacgtgaat
      181 gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc aagtttaaga
      241 aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc taaatagcgt
      301 tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg atgcgactca
      361 agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc gcgaattgac
      421 aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat tggatgttct
      481 tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg tatctaaaaa
      541 acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag aagttgttac
      601 tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag gtgttcgtgg
      661 atttatccca gcttcaatgt tggatactcg tttcgtacgt aacgctgagc gttttgtagg
      721 tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct tcatcctttc
      781 acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat tcggtaaatt
      841 ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg gcgctttcat
      901 cgaccttggt ggtgttgacg gattggttca cttgactgaa ttgtcacatg aacgtaacgt
      961 atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc ttgatcttaa
     1021 cgaagaagaa ggacgtgtat cactttcact taaagcaaca gtaccaggac catgggatgg
     1081 cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac gtttgactga
     1141 cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg tatcacaaat
     1201 ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag aagttcaagt
     1261 taaagttctt gaagttaacg cagatgcaga acgcgtgtca ctttctatta aagctcttga
     1321 agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc gtccacgtcg
     1381 tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag gattttcaat
     1441 ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa aatcctttgt
     1501 ttactaaaca agggattttt ctggctcttt gtcaactgta gtgggttgaa gtcagctaag
     1561 cttgagaaag gacaaatttc gtcctttctt ttttgatgtt caaagcgata aaaatccgtt
     1621 ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg cttgataagt ttgatgagat
     1681 tattggtcgc ttccagtttg gcattagaat agtgtagttg aagggcgttg ataatctttt
     1741 ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat aggatgaacc tgcttaagat
     1801 tgtcctcaat aagcccgaaa aatttctctg gttccttatt ctggaagtga aaaagcaaga
     1861 gctgatagag ctgatagtgg tgtttcaagt cttccgaata actcaaaagc ttgtttaaaa
     1921 tctctttatt ggttaagtgc atatgaaaag taggacgata aaatcgctta tcactcagtt
     1981 tacggctatc ctgttgaatg agtttccagt agcgcttgat agccttgtat tcatggtatt
     2041 ttcgatgaaa ctgattcatg atttgaacac gcacacgact catagcacgg ctaagatgtt
     2101 gtacaatgtg aaagcgaagc tgtttagcca agtcatagta agggctaaac atatccatag
     2161 taataatttt gacgcgacat cggacggctc tatcatactt aagaaagtga tctcggatga
     2221 cagcttgtgt tcttccctca agaacagtga tgatatcgag cttttcaaaa tcttgcgcaa
     2281 taaagctcat ctccatctcc cgattgaaac agtcactccc cggactgttt caacgttcca
     2341 ggacataatc tcaggaagac aagaaaaatc atgtttaaag tgaaaatcat tgagctttcg
     2401 aatgacagtt gaagttgaaa tggccagctg atgagcaata tcggtcatag aaatcttttc
     2461 aattaacttt tgcgcaattt tttgattgat aatacgaggg atttggtgat tcttcttgac
     2521 gatagaagtt tcagcgacca tcattcttga acagtgatag cacttgaatc gacgctttct
     2581 aaggagaatt ctagtaggca taccagttgt ctcaaggtaa ggaatcttag acggtttttg
     2641 aaagtcatat ttcttcaatt ggtttccgca ctcagggcaa gatggggcgt cgtagtccag
     2701 tttggcgat
//
LOCUS       DAVKZB010000156         2703 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKZB010000156 DAVKZB010000000
VERSION     DAVKZB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.156"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            31..573
                     /locus_tag="VXA80_001913"
     CDS             31..573
                     /locus_tag="VXA80_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV0354349.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(614..687)
                     /locus_tag="VXA80_001914"
     tRNA            complement(614..687)
                     /locus_tag="VXA80_001914"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Asn,seq:gtt)
     gene            complement(693..764)
                     /locus_tag="VXA80_001915"
     tRNA            complement(693..764)
                     /locus_tag="VXA80_001915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(729..731),aa:Glu,seq:ttc)
     gene            complement(816..1568)
                     /locus_tag="VXA80_001916"
     CDS             complement(816..1568)
                     /locus_tag="VXA80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0354350.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1565..2506)
                     /locus_tag="VXA80_001917"
     CDS             complement(1565..2506)
                     /locus_tag="VXA80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0354351.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDKDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTITPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
BASE COUNT          922 a          503 c          397 g          881 t
ORIGIN      
        1 tatagtctaa tttgtttaca aggaggaaat atgcaagaaa gtaacaaacg cttaaaaaca
       61 aagcgaacta ttgaaaacgc tatggtacaa ttactgatgg aacagccatt tgataaaatt
      121 tctactgtca aattagtaga aaaagccgga attagtcgtt ccagcttcta tactcactat
      181 aaggataagt atgatatgat tgagcactat caaagcaagc tattccatac atttgaatat
      241 atttttcaaa aacatgctca tcacaaaaga gacgctattt tagaagtttt tgaatatcta
      301 gagtcagaac cacttctggc tgcccttctt tctgaaaatg ggactaaaga aatccaaaat
      361 ttcttacgaa ataaacttca tatcatgctc agtacagatt tacaaaagcg atttatgcaa
      421 ctgaatctaa ataccactga attagaatat agtagcatct atctaactca tgcacttttt
      481 ggtgtctgcc aaacttggat tgcacatgga aaaaaagaaa gttctcaaga aataacagac
      541 ttccttatga aaatgcttgg tgatacaaat tgatacaaaa agaggaacac agctgtgttc
      601 ccttttatct atactccgcc agtaggactc gaacctacga catcatgatt aacagtcatg
      661 cgctactacc aactgagcta tggcggataa aatagtccgt acgggattcg aacccgtgtt
      721 accgccgtga aaaggcggtg tcttaacccc ttgaccaacg gaccttctat ctgtagcaga
      781 tataaccatt atatcaattt cttgctaatt gtcaatcact tttgagattt tttctctaaa
      841 atatctttta attttctaat ttttaatctt gaaataggac aacgatggtc ttcatagaaa
      901 acaatttcta agttttttcg atcaatttct ctgatattgc ctatatttac caaaaatgac
      961 ttgtgaggag aataaaatcg ctgagtatgt ttgtcctttt cctgaatatc tgtcatggta
     1021 ccataaaact cttttgcaaa attcttacca ataatgcgca atttatgaga tacccctgtt
     1081 gtttcaatat acaaaatatc atggtaagga atttttaaat catttccctt gtaattgtag
     1141 tcgaaataat ctacaacatc ttcattttca agtaacatac tcttcgtgta gaagatattt
     1201 tgctcaattc tcttcttaaa catctcatca ttgatatcct tatcaacaaa atctagggct
     1261 gatacctggt atttataggt tagagtcgca aactctgatc gactagtgat aaagacgata
     1321 atagcgtaag gattgtaatg acgaatgagc tgagccactt caaatccctt tttctcaatt
     1381 ccatgaatat cgatatctag gaaataaagc tgatttactt catcattttc aatgtattct
     1441 tcaaattcac ggacttttcc cgttgtcttg tatgatattg gaatattcga ttctttcgaa
     1501 atttcatcca atattctctc tagtctcact tgatgttcaa taacatcttc taaaattaaa
     1561 actttcattc aaattccctc ttaaatctaa tgatttgtct aaatgtactg ccttccatct
     1621 ctgtttctaa aataatattg ttatacttat ctagtagttc tttcacatta tttaatccga
     1681 ctccgcgatt tcttccctta gtggagaatc ctaaggcaaa tagatctcct gaaggagtta
     1741 tcgtcatttt acatgaattc tgaatcacaa taactgtttc agtttccatc ttaataactg
     1801 ctacttccat ctgcttttta tagctatcag ccgatccttc gacagcatta ttcaataaaa
     1861 cgctcatgat acgaaccaaa tccaatagtt caattggaag cttggtaatc gtatctttta
     1921 cttccagtgt aaactctaca ccattatttc gagcatagac aattgactga gcaaccaaac
     1981 ttcgtaaagc tgagtcttct atgttgttca aatcaaagta agtgtactta tctgaacgca
     2041 atttatgatt tgctttgact aaaacttcat tgtaaattct gtcaatttcc tgtaaattac
     2101 cactgtcaat tgccatctgc atgctgacaa gcattccagc ataatcatgt cgaaaaccac
     2161 ggatttcatt atacagacca acaatttcat ctgtgtaatt ctgtaaatgt ttctgttcaa
     2221 atttcttctg cttcaaagca atctctttct ccatttgaac tttatgagaa ttcattgcaa
     2281 agaaggtcaa aaggagagag ataaagacaa tagatgacaa aatacttcca aaactattca
     2341 aatgtttaat cgtacttacc atatctgaaa cgaaagatac aatatgtagc aatagtaaag
     2401 caaaaaatac ttttttcaag aaaggataaa ggtagtcctt gtcaaaatag gctagttcca
     2461 aatggaaata gtaaatgatt tttaatgtaa caaaataggt taacaccgtc acaacgaaaa
     2521 agaatgggaa atgatattgt aaaacaaaat tatctcctgt tatagaggag aaaattacgg
     2581 acagaaagtt atgagtgctc tcatataaaa gagatagtag taaacttagg aatagtcctc
     2641 tatccctctc atactgtttc atccatcgaa aataggaata taagcccaaa ggaaataaaa
     2701 atc
//
LOCUS       DAVKZB010000157         2655 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKZB010000157 DAVKZB010000000
VERSION     DAVKZB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.157"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            64..2190
                     /gene="pfbA"
                     /locus_tag="VXA80_001918"
     CDS             64..2190
                     /gene="pfbA"
                     /locus_tag="VXA80_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV0354352.1"
                     /translation="MKYFVPNEVFSIRKLRVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNKNKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKGHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNI
                     ELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYISFIKSNANGKE
                     PVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVIDSNQKNGEFNS
                     SKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKLPKTGSNKI
                     MELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2174..2470
                     /locus_tag="VXA80_001919"
     CDS             2174..2470
                     /locus_tag="VXA80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354353.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
BASE COUNT         1040 a          323 c          500 g          792 t
ORIGIN      
        1 ctatctgttt taagaaaagt aataatcaat tgttaaaaaa tagtaaaaaa attggaggtt
       61 ctgatgaaat attttgttcc taatgaggta ttcagtattc gtaaattaag ggtggggact
      121 tgctcggtac tattggcaat ttcaattttg ggaagccaag gtattttatc ggatgaagtt
      181 gttactagtt cttcaccgat ggctacaaaa gagtcttcta atgcaattac taatgattta
      241 gataattcac caactgttaa tcagaatcgt tctgctgaaa tgattgcctc taattcaacc
      301 actaatggtt tagataattc gttaagtgtt aatagtatca gctctaatgg tactattcgt
      361 tccaattcac aattagacaa cagaacagtt gaatctacag taacatctac taataaaaat
      421 aagagttata aggaagatgt tataagtgac agaattatca aaaaagaatt tgaagatact
      481 gctttaagtg taaaagatta tggtgcggta ggtgatggga ttcatgatga tcgacaagca
      541 attcaagatg caatagatgc tgcagctcaa gggctaggtg gaggaaatgt atattttcct
      601 gaaggaactt atttagtaaa agaaattgtt tttttaaaag gtcatacaca cttagaattg
      661 aatgagaaag ctacaattct aaatggtata aatattaaga atcacccttc cattgttttt
      721 atgacaggtt tatttacgga tgatggtgcg caagtagaat ggggcccaac agaagatatt
      781 agttattctg gtggtacgat tgatatgaac ggtgctttga atgaagaagg aactaaagca
      841 aaaaatctac cacttataaa ttcttcaggt gcatttgcta ttgggaattc aaataacgta
      901 actataaaaa atgtaacatt caaggatagt tatcaagggc atgctattca aattgcaggt
      961 tcgaaaaatg tattagttga taattctcgt tttcttgggc aagccttacc caaaacgatg
     1021 aaggatgggc aaatcataag taaggagagc attcagattg aaccattaac tagaaaaggt
     1081 tttccttatg ccttgaatga tgatgggaaa aaatctgaaa atgtgactat tcaaaattcc
     1141 tattttggca aaagtgataa atctggggaa ttagtaacag caattggcac acactatcaa
     1201 acattgtcga cacagaaccc ctctaatatt aaaattttaa ataatcattt tgataacatg
     1261 atgtatgcag gtgtacgttt tacaggattc actgatgtat taatcaaagg aaatcgcttt
     1321 gataagaaag ttaaaggaga gagtgtacat tatcgagaaa gcggagcagc tttagtaaat
     1381 gcttatagct ataaaaacac taaagaccta ttagatttaa ataaacaggt ggttatcgcc
     1441 gaaaatatat ttaatattgc cgatcctaaa acaaaagcga tacgagttgc aaaagatagt
     1501 gcagaatatt taggaaaagt atcagatatt actgtaacaa aaaatgtaat taataataat
     1561 tctaaggaaa cagaacaacc aaatattgaa ttattacgag ttagtgataa tttagtagtc
     1621 tcagagaata gtatattcgg gggtaaagaa ggaattgtta ttgaggattc aaagggtaaa
     1681 ataaccgttt taaataacca attttataat ttatccggta agtatatatc attcatcaaa
     1741 tctaatgcaa atgggaaaga acctgttata cgtgatagcg atggtaattt caatattgta
     1801 acggagaatg ggctttacaa aattgtaaca aataatttaa gtgataaaaa cgaaaaagaa
     1861 aaaaacaaag aggaaaaaca atctaattca aataatgtaa ttgatagtaa ccagaagaac
     1921 ggagagttta actcaagtaa agataataga caaatgaatg acaagatcga caataaacaa
     1981 gataataaga cagaagaagt aaactataaa atagttggag atggcagaga aactgaaaat
     2041 catattaata aatctaaaga aatagtagat gtaaaacaaa aattaccaaa gactggttcg
     2101 aataagatta tggaactatt cttaacagtg acaggaattg gtttactttt gacactaaaa
     2161 gggttgaagt attatggtaa agataaataa aatttgttcg atacaaggaa gttccgtaga
     2221 aaatgaggat attgtaggtt cacaaaatca gtatttttgg attattggtg gagctacaga
     2281 tttatataat tctaaagagg agataggtta ttcagtctca gaagttgtac atattttatc
     2341 agaaagtttg tcggttaatt gtaaagaatc aaaaactctt aaacaaatat ttgaaactgc
     2401 tttactcgag gttaaagatg aaatcggttt aaactcatat aaattaacag agtatagtaa
     2461 aatgaaataa taataggaca aatcaatcag gacagtcaaa tcgatttcta acaatgtttt
     2521 agaagtagag gtgtactatt ctagtttcaa tatactatat tatcgcttac caatatttgc
     2581 taaactttca gaaaaaaagt tggagtatat gatgctaggt gactgcgtca tgttagtaaa
     2641 cgaaatggaa ataac
//
LOCUS       DAVKZB010000158         2654 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKZB010000158 DAVKZB010000000
VERSION     DAVKZB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.158"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(157..723)
                     /locus_tag="VXA80_001920"
     CDS             complement(157..723)
                     /locus_tag="VXA80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354354.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(991..>2654)
                     /locus_tag="VXA80_001921"
     CDS             complement(991..>2654)
                     /locus_tag="VXA80_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0354355.1"
                     /translation="KVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYEDA
                     GKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYGVN
                     LICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNGGFAPYG
                     YKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPLFD
                     AGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDEVW
                     QAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKD
                     FYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVD
                     TSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKID
                     ELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQV
                     YEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
BASE COUNT          742 a          590 c          337 g          985 t
ORIGIN      
        1 gttatcaaat ctagtataaa ttatgagcgg ctactctaat actttccctc taaacaagaa
       61 aaaaacttac actcaagggt tttcttcccc cccttcgtta taacgttttg actcttttac
      121 tagcaaaggt atatactcac aaggaacttt ggttgactat tgaatctctc caacttcttc
      181 tttaacatat ccttctacat cttcaatctc tacaaacatt gggtctaagt gacacaagaa
      241 atgccaaact tcgatccctt tttttctgta aagaatcgct tcaccgtctt cacttccgaa
      301 aaagcttctg tcgatttcat atccgcggct ttctaagaag tcttttgctt tacgatagtt
      361 cgtttctctt gtttcgacat aggctttaac ttcatggttg ttaacgacat atgcatcaat
      421 ttttgaatat ccttcgatca ctctatcatt tttgagggat aaatttgaaa tctctttcca
      481 aataatgttt acattttcct caggatcgaa cataaattta gataaaggaa caatatttcc
      541 gttaaaaata atttccatat aatccggtat gtttttagga ttaaaatact ccacttcaaa
      601 accatcttct gtttccagag tgtatcccgg gatttgagcc acaaaggctt tcccatcttc
      661 tatggaatca aatgctacta aatctttaga ataatcattt tggtacaatt ccaatataac
      721 catcgataat ctctccattt tcattatcag gctaatgtaa ataagcacgt cacctgacca
      781 attcaggctc tctgtatcat ctcatcatat ttcctactta ctttacgagt cctataccca
      841 gaacacacct tatcgacctt cggtctcacc tcgtcgcatt ggctgaacat ctacttttac
      901 ttcgctgatg cttcaactcg tacaagcagt gataccgcct cagcgtgatg cgtcagtggg
      961 actcaaaaag tttggggaac cttttgagga ttaactacgt ttctctaata aacttacaca
     1021 ttcaacttgt tcatcattgt ccaaatctat gttgagattt tcttctataa ttggtagctt
     1081 aaaagtaatg gattttagcc attgtccgtt agattgtttt tcttcataaa cttgaatttc
     1141 agaaatcaaa gctgaaatta actgcctacg ctctacatca ttcatgactt tatagagctt
     1201 atcaaaatag atcagaacct tatatatgtt atctcctgta agtttttcag cttcaatagt
     1261 ctgtttcttt gctttcgcat caattagtga tgattctaat tcatctattt tgtcatacat
     1321 acgatatagt ctatcatcta aatcctgttt ccttctctta tagtgcttat cttcaacatc
     1381 taaattatct atttcctcaa ttagcttaaa ctttgtagaa tgactctttc tcaattcctt
     1441 ttggtaatta tctatttctt tttctatttc agaggtatcc accttcatgt tgattttttc
     1501 ttgcatcata gaagcaaatt tcggattact tactatcttg acaatcacct ctgcaacagc
     1561 atcatctaac aattcttctc taatttgctt actgaatgta cacttatgac ctcttatcat
     1621 ctgcctatgt ttacaaccat agtaataaaa atctttatac tttgtgccat ctttcttttt
     1681 cttgatacac ttgttcccaa acattcccac tccacatatc gggcatttta caattccaga
     1741 aagcaagtgt gtgcgtgtat cttttccttt attcacatgc tcatatttct ttgcttgaga
     1801 ttttagctta acctgagcag cttgccaaac ttcatcggaa actatagctt catgtatccc
     1861 ttcagatatt agatattcat cttgttcaac ctgcttatat tcatttcttg taccatgaac
     1921 tttttctaaa gttcttcttc caaatgctat tttcccatta tatacaggat tctttaatat
     1981 ctttcttata agacctgcat caaacaaagg attcttacca ttctgtcttg ggatttttct
     2041 aattccatga ttctctaagt atttagatat cccattggct cctatcgtag tatttacata
     2101 ctggtcgaaa atcgttctta ttgcaactgc ctcttcctca tttataaaca gcttgccgtc
     2161 ttcaagttta tatccatacg gagcaaagcc accattccat tttccttccc ttgctttttg
     2221 aatgcaacct tccattgttt gaatacggat gttttctctt tctatttcag ccacagctga
     2281 taaaacagaa atcattagtt tcccagcatc tttagatgaa tcaatgccat cttcaacgca
     2341 gataagatta actccataat cctgcattat ctgaagtgta gaaagaacat cagcggcatt
     2401 tcttgcaaat cttgataact taaacacaag aacaaaagat actccatctt ttccagattt
     2461 tatatcttcc atcattcgat tgaactgtat tctaccttca atagacttgc cagacttccc
     2521 ggcatcttca tactctccaa caatttcata atcgttgtaa atagcaaaag ctttcattcg
     2581 tgatttttgt gcctctaacg aatacccctc tatctgtatt gacgtagata ctcgtgtata
     2641 gaggtatact ttta
//
LOCUS       DAVKZB010000159         2648 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKZB010000159 DAVKZB010000000
VERSION     DAVKZB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.159"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            11..733
                     /locus_tag="VXA80_001922"
     CDS             11..733
                     /locus_tag="VXA80_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0354356.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKTKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VXA80_001923"
     CDS             730..1578
                     /locus_tag="VXA80_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0354357.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VXA80_001924"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VXA80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV0354358.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
BASE COUNT          823 a          562 c          503 g          760 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa gactaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgattctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagca
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat attctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaag
//
LOCUS       DAVKZB010000160         2646 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKZB010000160 DAVKZB010000000
VERSION     DAVKZB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.160"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            11..205
                     /locus_tag="VXA80_001925"
     CDS             11..205
                     /locus_tag="VXA80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0354359.1"
                     /translation="MDTVSKKRLKKTDVITMAGLTTNVMAQMGKDKPITFKNLERICK
                     ALSCTPNDIISFEDNFSDEE"
     gene            598..1623
                     /locus_tag="VXA80_001926"
     CDS             598..1623
                     /locus_tag="VXA80_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0354360.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1613..2620
                     /locus_tag="VXA80_001927"
     CDS             1613..2620
                     /locus_tag="VXA80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0354361.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLRISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          978 a          353 c          464 g          851 t
ORIGIN      
        1 ataaaccatt atggatacag ttagcaaaaa aagactaaag aaaacagatg taataactat
       61 ggcaggactt acaacaaatg ttatggcaca aatgggaaag gataaaccaa ttacatttaa
      121 gaatttagaa agaatatgta aggctttatc ttgcactcct aatgatatta ttagttttga
      181 agataatttt agtgacgagg aatagaaaat gactttaagg acagaagatc aagttaggga
      241 ttatgcaaga gaagtatagg ctttaatgaa gttgaagaaa acatcaatca aggtactggt
      301 caaataacta cttttaatca attaggcttc aagggatatt cagataagcc agatggttgg
      361 tatttaccta aaaatatgaa tgatgtagca ataatccttg aaacaaaatc agaagaaaga
      421 gatattagca aacaaatttt tattgatgag ttaatgaaaa atatagacat aatttaacta
      481 aaaataaaaa ctagatcctt ttttgaaaaa attatattat taaatttgta actgtatcta
      541 ttgacaatga taattattat cgatacaata gacttgaaat atgtttaagg agtttttatg
      601 aaaaacaaat tttttctaat agctatttta gctatgtgta tagtttttag cgcttgttct
      661 tctaattctg ttaaaaatga agaaaatact tctaaagagc atgcgcctga taaaatagtt
      721 ttagatcatg ctttcggtca aactatatta gataaaaaac ctgaaagagt tgcaactatt
      781 gcttggggaa atcatgatgt agcattagct ttaggaatag ttcctgttgg attttcaaaa
      841 gcaaattacg gtgtaagtgc tgataaagga gttttaccat ggacagaaga aaaaatcaaa
      901 gaactaaatg gtaaagctaa cctatttgac gatttggatg gacttaactt tgaagcaata
      961 tcaaattcta aaccagatgt tatcttagca ggttattctg gtataactaa agaagattat
     1021 gacactctat caaaaattgc tcctgtagca gcatacaaat ctaaaccttg gcaaacttta
     1081 tggagagata tgattaaaat tgattcaaaa gccttaggta tggaaaaaga aggtgatgag
     1141 ttaatcaaaa atactgaagc tcgtatatcc aaagaattag aaaaacatcc agaaatcaaa
     1201 ggaaaaatca aaggaaaaaa agtattattt actatgatta atgctgcaga tacatcaaaa
     1261 ttctggattt atactagcaa agatccaaga gcaaattatt taacagattt aggtctagtt
     1321 ttccctgaat cattaaaaga atttgagagt gaagatagtt ttgcaaagga aatttctgca
     1381 gaagaagcaa ataagataaa tgatgctgat gtaatcataa cttatggtga tgataaaact
     1441 cttgaagctt tacaaaaaga tcctctttta ggtaaaataa atgcaattaa aaatggtgcc
     1501 gttgctgtaa ttccagataa tacaccgtta gcagcctcat gcactccaac accactttca
     1561 ataaactata ctattgaaga atacctaaat cttttaggaa atgcatgcaa aaatgcgaaa
     1621 taaaaaacaa ataaacctag gcataatttt tgtaatctgc ctaggtcttc ttattacaat
     1681 atttttgtca ttaaagcttg gaacaaaaga aattaatatc agagattttt tagcagcttt
     1741 tggaatgggc aatacaaatg atgattttat taaatcaatt atatataaaa gaatacctag
     1801 aactattttt gcaattttag caggttctag tcttgccata agcggtgtat tgatgcaatc
     1861 agttactaga aacccaatag ctgatccagg tatactcggt ataaacacag gagcaagtct
     1921 tagtgtagta attggtcttt cttttttaag aatttcatca agcataagcc atataagttt
     1981 tgcaatcatt ggtggcttag taagtgcaat ttttgtatac gcgattgctg taagcggaaa
     2041 agcaggcctt acccctataa aacttgcctt atcaggaact tgtgttagta tggctttaag
     2101 cagttttgta agttttttaa ttttaccgaa taataacgtc ttagacaaat ttagattttg
     2161 gcaaataggt agccttggag cagctacatt atcttctata tctacactac taccttttat
     2221 aattttaggt cacttgatag ctatatttat ttcatcagat ttaaacgctt tagctatggg
     2281 tgatgaaatg gctgttggtc ttggagttaa tgttaatagg ataagatcac ttgcaataat
     2341 tgcaagtgtg cttttatgtt caagtattac tgcaattggt ggacctattg gcttcgtagg
     2401 tcttatagtt cctcactttt gtggcttatt tataagcaaa gatatacgca caatgaccat
     2461 ttcttcatct tttataggtg cagagctctt gcttatatgt gatataatcg gccgtatgtt
     2521 aggtaaacca ggtgaaattg aagtagggat aattactgca ataatcgggg gtccagtact
     2581 tatttatgta actatgaaaa atagaggggt taataactaa tgcaaaattt aattataggt
     2641 attcaa
//
LOCUS       DAVKZB010000161         2592 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKZB010000161 DAVKZB010000000
VERSION     DAVKZB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.161"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            10..1233
                     /locus_tag="VXA80_001928"
     CDS             10..1233
                     /locus_tag="VXA80_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0354362.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNIIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            2048..2218
                     /locus_tag="VXA80_001929"
     CDS             2048..2218
                     /locus_tag="VXA80_001929"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354363.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
BASE COUNT          950 a          489 c          366 g          787 t
ORIGIN      
        1 aggaaacaaa tgattacaaa acaatcagcc tttttacaaa atagagccac tatactggag
       61 tttctatatc gaaatccagc aacatcccgt acagatattg ttaatgaaac aggacttact
      121 ccagcaacaa cgactaacat cataaaagag ctaagcgaac agtccctcat atacgaaact
      181 ggtgatgaat ttagcgagtt ttccggatct ggaagacgta gaaaaactat ttctatcaca
      241 gataatattc cgtatgttgt tggaggtatt gaaataaacg tcctaggtat ctttctcagt
      301 ctatgtgact tacaagggaa aactcttttc gagacagaaa ttttgaatga agattatcct
      361 atttcagaaa tcaattccac cattaccaat atgataaaaa cagctataga gtacgtccct
      421 ttggaaacaa aattacttgg atttggctta tcaatacctg gacattataa caaagactcc
      481 ggaagtatca ttacaaacaa ccccatatgg gaatctttta atttattaaa tataattaaa
      541 agattcaatt ttccttttat tgtaaaaaat aatatcgatt gtatggctat aggacaatac
      601 ctttttaatc cacacaatac ccccgataac tttattttcc tacacgctgg attaggtatt
      661 tacacttcct ttttcacaaa agaaaaaata ggagcctcta aaaatcctta tatcggagaa
      721 attggacaca ccattgtcga attgaatggg caatattgtg aatgcggaaa aaaaggttgt
      781 ttacaaacat atatttcgga tgcttggtta atcaaacacg cccaattatt atttaaaaat
      841 tcccaactaa ctgtactaaa aagccttgta aagactgaaa aagacattca tttagacacc
      901 cttttaacgg cttataattt aggcgactcc gctttacgtc aacaaattga taaaggagtc
      961 aatttattag ccacttctat tgcaaatctc ctcctcatca atcctgctga taaaatctat
     1021 atcaacagtc aattgcttaa ttatcaacct ttcactcatg aagtcaggga taaaatccaa
     1081 gaccagctcc acttcgttcc ctttactcgt aatatagaaa ttgaaatttt accttacaac
     1141 aaacatcgtg gaagtatagg agcttgtgca ttagctatcg tcgctttttt catagaacat
     1201 agcaatgtat tacaagatat tatttcacct taatatatta gaaatctata gacctgttta
     1261 aatcaactat aacctgtagt agatatctcg tatttagaca atatgaaaac aagacgactt
     1321 ccatatagga aaccgccttc tcgctatgtt gagtgattta tattaaaata acttttcttc
     1381 tagctgcatt ttattattat aaaaacattc atcataaccc ccagaactta aataacaatt
     1441 tttattcaag atacatactc ctagaataaa ctttatatga aatccccatt tttgttttta
     1501 caattctccg tatactcaat gaaaatcaaa gagcaaacta ggaagctagc tgcaggctgc
     1561 tcaaaacact gttttgaggt tgtagataga actgacgaag tcagtaacat ctatacggta
     1621 aggcgacgct gacgtggttt gaagagattt tcgaaaagta ttagttaaat cttgtttaat
     1681 atctctacac catactattt tacatatatt atttagcgcc acatagtact gaactctctc
     1741 caaaaacggt tatgcctctt tgaatagggc gttatcacaa gaaaagcatc tccacgtttc
     1801 aacttcatat ggctcaaaaa caatcaattg atgctaaaac ctgtacctag atgtttcggt
     1861 tcataaaacc atgaaactgt aaaagtggat gaaattgata gcgatagtca aatcaagagg
     1921 catcataact ctaaaaagtc acaatatata agttcatcct cggaaaaata tcattctaat
     1981 tgttgaaatg cctacatgaa aagaaacgtc aaatgctcat gaaacaacga atacaggtat
     2041 caaaactatg acaaaacaag tccctaaatt tactaaagac actgctcaac tttacacctg
     2101 taaatggttg ttgtataata aagttacaaa gatgtacgac cacactgttg taaatcatag
     2161 tgttcgcgaa tatattactg atagcatttc tacaaataca attgaagaga ctggatagac
     2221 tttaaacgaa atatgtcagt gttttggcat tcctagcctt catatcattt aaagaattct
     2281 atagacaaaa tttttcccaa tacagacact cgtaacaact gcttcatttt tctaccaaca
     2341 tatttaggaa caggataaga tacaagagga ttaatccata gatcagttct ataccaatct
     2401 aagacaaata agctaaaaaa acgattgata ataagcaaat agattccaaa ttttctctat
     2461 ctgctcattt taataaacaa tactagtgta actatccttc cagtcagaag cttgtcaaat
     2521 cacaccgaaa attcttctaa aatttatctc gttaggcaat caagcaaaaa ctcgacgata
     2581 gtacaaacat ta
//
LOCUS       DAVKZB010000162         2546 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKZB010000162 DAVKZB010000000
VERSION     DAVKZB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.162"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            44..1798
                     /locus_tag="VXA80_001930"
     CDS             44..1798
                     /locus_tag="VXA80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354364.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAILTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGL
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1782..>2546
                     /locus_tag="VXA80_001931"
     CDS             1782..>2546
                     /locus_tag="VXA80_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354365.1"
                     /translation="MKEFYKKRFALTDRGARNSSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLA"
BASE COUNT          907 a          341 c          466 g          832 t
ORIGIN      
        1 gaatttatat ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa
       61 actatttgct tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc
      121 tatatctgct atacttacag tatatggata ttatttaatc tacaaatttc tagataagtt
      181 aataattaat tcaaatttat ccggtgcaga gagcatagca ttaaaatctg ttattacact
      241 aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacacatct tgggatttag
      301 gcttgaaaca aatttaagaa aaaggggaat tgatggctta gagaaagcaa gttttaggtt
      361 ctttgactta aatccatctg gtcaaataag aaagattata gatgacaatg ctgcacaaac
      421 tcatcaggtg gtagcacaca tgattcccga tagttctcag gcaataatca cacccgtact
      481 tgtacttgca cttggcttta tagtaagtat aagagttggc ttaattttgc ttgctcttac
      541 tataattggt ggcttaattt taggggcaat gatgggcgag caagaattta tgaagatata
      601 ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt
      661 tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg cgataaaaga
      721 ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca
      781 atggttattt tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag
      841 cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt
      901 tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta
      961 tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg
     1021 taatgtcaat aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa
     1081 tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact
     1141 tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa
     1201 tgttaatgaa ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc
     1261 cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta
     1321 tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa
     1381 attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg
     1441 ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat
     1501 tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa
     1561 cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat
     1621 tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa
     1681 aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct
     1741 gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa
     1801 aaagatttgc tcttacagat agaggagcaa gaaattcaag taaagcaaca ctggcttcat
     1861 ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag
     1921 ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga
     1981 tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag
     2041 aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact
     2101 tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag
     2161 agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa
     2221 tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta
     2281 ttttaagctt tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat
     2341 attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg
     2401 agattaaagc atataattta tcgaaggata ttaaagatga cttatataaa aaaatggaag
     2461 atagtgagag aatacactta aaggcggaag taactacaat tttaactttg tctatatctt
     2521 caatatttag ctttatatct cttgct
//
LOCUS       DAVKZB010000163         2460 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKZB010000163 DAVKZB010000000
VERSION     DAVKZB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.163"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..868
                     /locus_tag="VXA80_001932"
     CDS             <1..868
                     /locus_tag="VXA80_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354366.1"
                     /translation="KDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFD
                     IDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQI
                     LIDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANC
                     TDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEK
                     KIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNL
                     IEKTKMAEEFIY"
     gene            879..1463
                     /locus_tag="VXA80_001933"
     CDS             879..1463
                     /locus_tag="VXA80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0354367.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMTLVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1463..2158
                     /locus_tag="VXA80_001934"
     CDS             1463..2158
                     /locus_tag="VXA80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0354368.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKIRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2146..>2460
                     /locus_tag="VXA80_001935"
     CDS             2146..>2460
                     /locus_tag="VXA80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354369.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFY"
BASE COUNT          882 a          323 c          469 g          786 t
ORIGIN      
        1 aaaagactct ttagatgcat ctaaagaggg cttgatggaa atattttatt tatcgcccaa
       61 aatagaaaga ttaaaagaaa ttcaaaatca agatttacaa gaaggcgatg actatagctt
      121 aaaaaaattt gatattgatc taaaagatgt tgagtttgcc tacaataaag acgcaaaagt
      181 tttaaatggt gtaagtttta aagctaagca gggagaggtc actgctttgg taggtgcaag
      241 tggctgcggt aaaacaacta tcttgaaact tatatcaaga ctttatgatt atgacaaggg
      301 acaaatctta atcgatggca aagatataaa agaaatatca acagaatctc tttttgataa
      361 ggtctctata gttttccaag atgtggttct ctttaatcaa agcgttatgg aaaatattag
      421 aatcggaaag caagatgcaa gtgacgaaga ggttaaaaga gcagcaaaac ttgcaaattg
      481 cacagatttt atagaaaaaa tggataaagg tttcgataca gttattggtg agaacggagc
      541 tgagctatca ggaggagaaa gacaaagatt atcaatagcc agagccttct taaaagatgc
      601 gccgatattg atcttagatg agataacagc aagccttgat gttaacaacg agaaaaagat
      661 tcaagagtct ttaaataatt tagttaaaga taaaactgtt gtaatcattt cacatagaat
      721 gaaatccata gaaaatgcag acaagatagt agttcttcaa aacggaagag tagaaagcga
      781 aggtaagcat gaagagcttt tacaaaaatc aaaaatttac aaaaatttaa tagaaaagac
      841 aaaaatggca gaagaattta tttattagga ggactacaat ggataataaa aaattaaaag
      901 taaaagattt agtaagtatc ggtgtttttg gcgtaattta ttttgccttc atgtttggag
      961 ttggtatgat gggcttgatt ccaatattgt tcttaatata cccgacagta ttagccatag
     1021 ttgcaggaac tgttgttatg ttatttatgg ctaaggttca aaagccatgg gcactattta
     1081 tatttggtat gatatcacca cttgtgatgt ttgcagctgg tcatacctac gtagttgtgg
     1141 ttttatcact tatagtaatg ataatagcag aattaattag aaagattggt aattataatt
     1201 catttaaata caatatgctt tcttatgcaa tcttcagcac atggatatgt agctctttaa
     1261 tgcaaatgct tttagcaaaa gaaaaatata tggagtggtc tttgatgacg atgggaaaag
     1321 attatgttga tgtattagaa aagttaataa cttatcctca catgacttta gtagccttag
     1381 gtgctttctt aggaggaatt cttggagcat atataggcaa ggctctattg aaaaaacact
     1441 ttgaaaaagc aggcatcgta taatgcctac tttttcttca aatgctccct ttaatcccaa
     1501 tccgatctct aagatattag tcgtatttat cacaggctta actgttatgc atagcataaa
     1561 catccgtttt gagctggcaa ttgtttgtct tattggtatt ttgctttatt tgaatggata
     1621 caaaaaaaca cttttcaaat ggattatatt gtgtgggata ctttactcct tgcctaattt
     1681 tatggtgtta tctgaattaa accctatagt taagatgttt ttgagtatac ctattgttat
     1741 tagaatgttt attttaccat ttatggcagc aagctttatg ataaagacct cggatgtagg
     1801 cgcaataatt tcatcgatgg ataagcttaa gatttcaaag aatgtatcca tacctattgc
     1861 ggttatgttt agattcttcc catcttttaa ggaggagaag aaaaacatca aaatggctat
     1921 gagagtaaga gggataaatt ttaaaaaccc agtcaaatat cttgaatatg tttctgtgcc
     1981 actactcatt atatcatcta atatatcaga tgacattgca aaagcggcag aaacaaaggc
     2041 aatagaaaat ccaattgcca agatcagata cattcgcgta aagatacagc taattgattt
     2101 tgtttatgtt ttagcggttg ctggacttat tgtgggaggc ttaatatggt tgaaataaaa
     2161 aatttaagtc ttgattatgg tgaagagcat atattagatg atatatcact atccatagcc
     2221 gagggagagt gcgtgctatt tacaggaaaa agtggaaatg gtaagtcatc tttaataaat
     2281 tcaatcaatg gactagctgt aaggtatgac aacgcaaaga caaagggcga aataattatt
     2341 gatggtaaga atataaaaaa tttggaactt tatcaaatct caatgcttgt ttcaactgtt
     2401 tttcaaaatc ctaagacata tttttttaat gtcaatacga cattagaatt attattttat
//
LOCUS       DAVKZB010000164         2369 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKZB010000164 DAVKZB010000000
VERSION     DAVKZB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.164"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            43..1050
                     /locus_tag="VXA80_001936"
     CDS             43..1050
                     /locus_tag="VXA80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0354370.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1053..1253
                     /locus_tag="VXA80_001937"
     CDS             1053..1253
                     /locus_tag="VXA80_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0354371.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1405..1587
                     /gene="rpmF"
                     /locus_tag="VXA80_001938"
     CDS             1405..1587
                     /gene="rpmF"
                     /locus_tag="VXA80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV0354372.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1603..1752
                     /gene="rpmG"
                     /locus_tag="VXA80_001939"
     CDS             1603..1752
                     /gene="rpmG"
                     /locus_tag="VXA80_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0354373.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2232..>2369)
                     /locus_tag="VXA80_001940"
     CDS             complement(2232..>2369)
                     /locus_tag="VXA80_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354374.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT          784 a          459 c          438 g          688 t
ORIGIN      
        1 aagtgatatc atttagatat caaataacag gaggtcattc ttatgactga aaaactaatc
       61 aattcaaaac caaatggtgt attcgcattg attctcattg agttgacaat cgtacttggt
      121 atctttatat ttataatggg ggttggttcg gaaaacattt ttggaattat tatcggacct
      181 ttactaatcg taattgcagg tctagctcat gctggtttaa aagttgtcaa acctcaggaa
      241 gctctggttc tgacactctt tggtaattat acaggtacca tcaaagaacc tggtttttac
      301 tttgtcaatc ccttcagcgt agcagtcaac cctgcaaacc acactcgact tggacaaagt
      361 ggtgatgtta gcacaaaatc tcctttttta ggagctaaat catcaaatga caatgatgta
      421 aatcttgaaa ttggcaagaa acacatttcc ctcaaagtca tgaccttgag caattctcgt
      481 caaaaaatca atgattgctt aggaaaccct gtagaaatcg gtatcgcggt aacttggaga
      541 gttgtcgata ccgctaaggc agtcttcaac gttgataact acaaagaata tctttcattg
      601 caatgtgata gcgccctccg taatattgtc cgcatctatc cttacgatgt gtctcctaat
      661 gtggatacta cgggtgatgg gcaagcagat gaaggtagtc tccgtggctc tagcgaaatt
      721 gttgctaacc gtattcgtga agaaatccaa agtcgtgttg aggatgctgg cttggaaatc
      781 cttgaagcac gtatcactta cctagcttat gctccagaaa ttgctgccgt tatgcttcaa
      841 cgccaacaag catctgccat tattgatgca cgtaagatga ttgtagatgg tgctgtagga
      901 atggttgaaa tggcactaga acggctcaat gaaggggaat tggtagaact tgacgaagaa
      961 cgaaaagctg ccatggtttc aaatctcctt gtcgttcttt gtggcaatca tgatgcacaa
     1021 ccaattgtca acacaggaag tctttactaa agatggctga agctaaaaaa aagcagatcc
     1081 cccttcgact ctcaacaaag ttatacgctg cactcgcatc atgggcagaa gacgactttc
     1141 gttcagtcaa tggccaaatc gaatatctcc tcacagagtg tgtcaagcaa cggaagaaag
     1201 acggaaaata cgtatcagaa accattgacg aaccatttga gattgatatt taacaccttc
     1261 tcctgtctgc cgtgtcggat aggagaattt tttcatcttt ttttgtcaag taactttact
     1321 ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt gaatcgaata
     1381 taaataccta aaggagaaat caaaatggca gtacctgcac gtcgcacttc aaaagcgaag
     1441 aaaaacaaac gtcgtacaca ctacaaagta acagctccat ctgtaaactt tgacgaaact
     1501 actggagatt actcacgttc tcaccgtgta tcacttaaag gatactacaa aggacgtaaa
     1561 atcgctaaag ctgcatcagc tgaataatag aagggagata ccatgcgcgt aaatattaca
     1621 cttgaacaca aagaatctgg tgaacgcttg taccttactt ctaaaaacaa acgtaacact
     1681 ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt tgtgtttaca
     1741 gaagtgaagt aagcattcaa tacgaatcaa tacagaagaa aaacgcttga tcagcgtttt
     1801 ttctttttgt taatttcact atattgcaag agaaatcatt cgaatgacta cattttttga
     1861 aataagaata cggattcgga cgattaaaag taagagaaac tcaaatgtaa gttggggttc
     1921 cttttttgta tatttttcaa ataacgcctc tacactattt gtagcaaatt caccaactac
     1981 agttgcatct tagttaaaat aagttagaat atgtaagtga gtaccagata taccaagaca
     2041 tcgtcaccat ctaaggtata ttcaaaatac aaaagttgac caactagatt tctgaatatc
     2101 cttatatatc cattcttaaa attggtttaa atagcgtagt cttttaaact agttttgaga
     2161 atccaaaaaa tcttcctaca tatgtaagaa gattttttag ttcagaatga ttagatttag
     2221 ctaatggata cctatcctac ccactcaccg ttagcattga ctcgatagcc atcgggtgta
     2281 gtcgtgttta ctaataaatc tcctgaatta taggtatagt accatttacc agaaactgta
     2341 aaccaaccag tagccattga acctgattc
//
LOCUS       DAVKZB010000165         2306 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKZB010000165 DAVKZB010000000
VERSION     DAVKZB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.165"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..1289
                     /locus_tag="VXA80_001941"
     CDS             <1..1289
                     /locus_tag="VXA80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV0354375.1"
                     /translation="FLPIIIFILGIIFGLKPGKALMSGITVGIGSIGLGLVLNLLSGS
                     MGSAIQDLGNHYGSSLNILDIGVGVGGPLAFSTSLGLLMIPISLVVNIFFVFLGLTRT
                     LNVDIWNFWFPIFMGMITQSITGNFVFGILGGTVAVMIQWLLADINQKKNSEFFGYPG
                     IAISHMMVNSGAIIARPLNWIFDRIPGLNKIDLDTESLTKRFGILGDTVFIGLVIGAL
                     VGIFAKYDVSKIGNLAVSTAAVMKIMPKMVAMFMEGLGPIAEAASDFANNKLGGRQVN
                     IGMDAALVVGHSAVMSTSILLVPISLALALVLPGNQVLPFGDLAFFAFAIAMMVPFFK
                     GNVIRTVLGSAIYVVACLYLSTWLAPVMTDVFNLASFDVGTAGLVTMLLAALWPVALY
                     VLALKYLGIFGVVAIGILVILIMYYINKIKKISYFQ"
     gene            1321..1605
                     /locus_tag="VXA80_001942"
     CDS             1321..1605
                     /locus_tag="VXA80_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0354376.1"
                     /translation="MKKILVICGAGHATSTIAVSKIKKWLEDNNLDSDAKIYQSKIAD
                     ELNKFDDYDVVVSTTVVPDSVKEKVINGVALLTGVGIDKVFEQIRKELEK"
     gene            1605..2126
                     /locus_tag="VXA80_001943"
     CDS             1605..2126
                     /locus_tag="VXA80_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEV0354377.1"
                     /translation="MIIHDFIKLETVAGRPSFHDITDDIKEIVEQNSITDGIIVISSS
                     HTTCSLFFDEDMHDRNYFGDDFLHVDIAKMMDKIAPPMRSEGDYHSPGEKHVEFGLNL
                     CDSEFPPEKWTMLNTDAHLKSSLFGSNSMTLIIKDKKILLGKLGKVFFVDWDHLRERD
                     RNISILLLGEKNG"
     gene            2110..>2306
                     /locus_tag="VXA80_001944"
     CDS             2110..>2306
                     /locus_tag="VXA80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0354378.1"
                     /translation="MKKMVNILPSILNSDFWNIKETLNILNNSETKEIHIDIMDGNFV
                     EDMAFGPKFVRTIREHTDLKL"
BASE COUNT          790 a          281 c          447 g          788 t
ORIGIN      
        1 tttttttacc aattataata tttattttag ggattatctt tggtctaaaa ccaggaaagg
       61 ccttaatgtc aggaattaca gtaggaattg gttcaatcgg tcttggacta gtattgaatt
      121 tattatcagg tagtatggga agtgcaattc aagatctggg aaatcattat ggaagctctc
      181 ttaatatatt agatataggt gttggagttg gaggaccttt agctttctct acatctttag
      241 gcttgctgat gatacctatt tcacttgtag ttaatatatt tttcgtattt ttaggcttaa
      301 ctagaacttt aaatgtagat atttggaatt tttggtttcc tatatttatg ggaatgatta
      361 cccaatctat taccggtaac tttgtgtttg gtatactagg tggaaccgtt gcggtaatga
      421 ttcagtggtt acttgcagat ataaatcaga aaaaaaatag tgagtttttt ggctaccctg
      481 gtatagccat aagccacatg atggttaatt caggtgcaat aattgcccgt cctttaaatt
      541 ggatttttga tagaatacct ggtctaaata aaattgatct tgatactgaa tctttaacta
      601 aaaggtttgg aatattgggg gatacagttt ttataggatt agttataggt gcactagtag
      661 gtatttttgc taaatatgat gtatctaaga tcggaaatct tgctgtatct acagcagcgg
      721 taatgaaaat aatgcctaaa atggttgcta tgtttatgga aggtctggga ccaatagcag
      781 aagctgcctc agattttgct aataataaat taggtggtag acaagttaat ataggaatgg
      841 atgctgcact agtagtagga cactctgcag ttatgtctac atcaatatta ctagttccaa
      901 tttctttggc tcttgcatta gtattaccgg gcaatcaagt tttacctttt ggagatttag
      961 catttttcgc atttgctata gctatgatgg ttcctttctt taaaggaaat gttattagaa
     1021 ctgtactagg aagtgctata tatgttgtag cttgtctata tttatcaaca tggttagctc
     1081 cagtaatgac agatgtattt aatttagcta gtttcgacgt tggaacagca ggattggtta
     1141 caatgctact cgctgcttta tggccagttg ctttatatgt attagcttta aaatatttag
     1201 gcatatttgg agtcgtagca ataggaatat tagtaatact aataatgtat tacatcaaca
     1261 agattaagaa aatttcatat tttcaataga atataaatat aaaatataaa ggagataaaa
     1321 atgaaaaaaa tattagtaat ttgtggagcc ggtcatgcaa catcaaccat agctgtttct
     1381 aaaataaaaa agtggttaga agacaataat ttggattctg atgcaaaaat ttatcaatca
     1441 aaaatagctg atgaactaaa taaatttgat gattatgatg tcgttgtaag tacaacagtt
     1501 gtaccagatt cagtaaagga aaaagtaatt aatggagttg ctttattaac tggtgttggt
     1561 atagataagg tatttgaaca aatacgcaaa gaattggaaa aataatgatt atacatgatt
     1621 ttattaagtt agaaacagtt gcaggaagac catccttcca tgatataaca gatgatatta
     1681 aagaaatagt agagcaaaat tctataacag atggaataat tgttatatca agctctcata
     1741 caacatgttc attatttttt gatgaggata tgcatgatag aaattatttt ggagatgact
     1801 ttctacatgt tgatatagct aaaatgatgg ataaaatcgc cccaccgatg aggtctgagg
     1861 gcgattacca tagtcctgga gaaaaacatg tagagtttgg attaaatcta tgtgattcgg
     1921 aatttccacc agaaaaatgg accatgttaa atacggatgc ccatctaaaa tcttcactat
     1981 ttggcagcaa ttcgatgact cttataataa aagataaaaa aatcttatta ggaaaacttg
     2041 gtaaagtatt ttttgttgac tgggatcatt tgagagaaag agatagaaat attagcattt
     2101 tattgttggg tgaaaaaaat ggttaatatt ttaccttcga ttctaaatag tgatttttgg
     2161 aatatcaaag aaacattaaa tattctaaat aatagcgaga caaaagaaat ccatatagat
     2221 ataatggatg gaaattttgt tgaagatatg gcatttgggc caaagtttgt aagaactata
     2281 agagaacata cagatctgaa attaga
//
LOCUS       DAVKZB010000166         2253 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKZB010000166 DAVKZB010000000
VERSION     DAVKZB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.166"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            235..609
                     /locus_tag="VXA80_001945"
     CDS             235..609
                     /locus_tag="VXA80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV0354379.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            817..1719
                     /locus_tag="VXA80_001946"
     CDS             817..1719
                     /locus_tag="VXA80_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354380.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            1775..2128
                     /locus_tag="VXA80_001947"
     CDS             1775..2128
                     /locus_tag="VXA80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV0354381.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYYIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
BASE COUNT          744 a          285 c          428 g          796 t
ORIGIN      
        1 agtcaaaaaa aataaatttt gaaatagtat tagtgagttc gcaatcattt attgctagtg
       61 gtttagaact gttaagtttc aaacaataat tgattttaaa ccttaggtaa cctataaaaa
      121 caaataaggt agagaaagga tatcttatct aggtagctaa gtgataaaaa cacagtcgtc
      181 tatcacttgt taggaaacta tcctaactat gcttaataaa aaaggagaaa ataaatggaa
      241 ctcgtattac caaataatta tgttgttctt gagcaagaag agatgatgta tcttgatggg
      301 ggattttcta ttctgagatg gcctgttgca acagccatta atatagcttt taatggtgtt
      361 ttaggtggag gagcaatcag tctagttaga aattatattc gtaattatgg tttgcggcga
      421 gttacaagcg caattgctgg agcagctgca agatatgttg gggtacgagt tgcaaataga
      481 gtggcaggat ttgcactgtc tgctattaat ggatttgcag cttggatgtc aattggcgat
      541 gctattacaa caatctgggc caataatgat gtaaatagga gagacccaaa tttaaacgcc
      601 ttgtggtaac tagatatggg tattaaaatg gaaaaaattt ttgttattat tttttttgta
      661 tgtctattta tatcatccat aactttttta gcctatgatt ttgttagcga agaaataaaa
      721 aagttgatta tttggattaa tgtcgttttt ttgattttaa tcatagcaat gatgatttat
      781 ccaaaattaa gaaaatgata aggacactgt aaaattatga tacgtaaact aattgttttc
      841 ttactaatgc tccctctttt tgtcttgtgg ctagagttgc atgtcctagt gaataactta
      901 ctattgaatc tagaatctcc ccttgatttt gtgattagta tgagtcttgc tttctgtagt
      961 ttgattttat caaaaatagt tttagacata ctatacgcat tgaaagattt gtacaagaaa
     1021 gaagctctta ttacgatttt tccttttatt tttataggca ggaagaaagt aaatgtgagg
     1081 ttctcgccct atttttcatt ccatcgtaaa agtttgtcgc ctgatgattt aagatctagg
     1141 attatatgga gttttattct tgaaattgct attatcctag tatttatttt aaaaattcct
     1201 tttgcaatca tcatgcttac tacaatattt ttctggacta taatggatat caatcatctc
     1261 gtttttaata agacggaatt tctttttaat caaaacaaat ggcaaaaaga agattcgttt
     1321 gaaagtgatt tgacgaaaac gttaaaggat aagatacaaa aatcagaatt aagctattct
     1381 gacttaatgt cccttcttct ctatgatgcg atgaaccaat ccaccttttt aacggatagt
     1441 gaactttttg aggatatttt gaaaaaaatc gaagattctc ataacaccct tctgtgtaca
     1501 ggtcttgtcg agttactgct gtatgaaatt tctattagta agaatcataa ttggtcagaa
     1561 aagattgata aaattaggat agagcttatc aagattaatc aattagattt tttctattat
     1621 acaagttggt taaggcaaaa ttttgatttc tgtatgaata gagagtatca taaaatgaaa
     1681 tctcggaaat tattactgag taataaaaag atagtttaag ttcgtcctat ttgtaaaaca
     1741 aatgaaacaa tttcaactaa ggaggagaaa acaaatggaa cttgtattac caaataatta
     1801 tgttgtgatt gatgaagaag taatgatgta tcttgatggg ggagcttatt taagcaagcg
     1861 tgcttgtcaa ggaatttgcg cagctttagc tatgagttca ggaactttta tagcattagc
     1921 tggagctgca gttttaacca aaaaactaat aaactatatt aagtttggag gtcttggagg
     1981 ctggcttatt ggtgcagcag caggtgtatt ggctacagca gcagggaaaa tagcttacta
     2041 tattggatat ggtgttctta atagaggttg tgatattaac gggaaccctt atccttggga
     2101 tggattcata tctgcaacag taagatgagt aatgtagata agattcgaaa aatacatatt
     2161 atcgtatgtt ggatgtatat atttttatca ttcagggcga ttataaatga cacggagtat
     2221 tttttgctta tttttttagc ttttatctac tca
//
LOCUS       DAVKZB010000167         2223 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKZB010000167 DAVKZB010000000
VERSION     DAVKZB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.167"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(306..1199)
                     /locus_tag="VXA80_001948"
     CDS             complement(306..1199)
                     /locus_tag="VXA80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0354382.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICSDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(1953..2183)
                     /gene="blpU"
                     /locus_tag="VXA80_001949"
     CDS             complement(1953..2183)
                     /gene="blpU"
                     /locus_tag="VXA80_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV0354383.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT          740 a          462 c          246 g          775 t
ORIGIN      
        1 acttttatat cactaagact caataaaaga taatgtctat ttaactttta tacgattact
       61 acatcattga aaattgagtt tcatcctcta tactaatgta ggaaacctat taaaacattc
      121 ctttaatcca atataagaat accttgttcc attaatctct acccgatatt atagatagaa
      181 ccaaattcct atacttaaaa ggacaaataa cgttccaacc ataataaacc tcctttcttt
      241 ttgagaacgt tttctttata ggtttagtat agcaaaaaca tgaaaaaaac aacatgactt
      301 agaagtcagg ttgttgggaa atctatctgt ttttctgcta aaattctctg aactaatacc
      361 tgatcgtcta gcaattctgc tagagaaatc gccttttcat agaaactttc tgcctctaaa
      421 atattcttgt ctacaaataa agcatatttt gcctttagca aaaatagatg aggtcggtaa
      481 aaaacatgtc ttgttttagc aataattttc tcaaatgttt gaatatatat cagactatct
      541 tctgtctgaa tttggataaa taggctcaga agaacttttt ccatctgaac taggctatcc
      601 ttatcttcat attctgaaca tagcaatact ttttgagcta actcctcaaa atgttcttta
      661 tcttctaatc ccatagcaca acaaaagaaa tacaattcaa taatcaacaa atcatttaca
      721 gaaaaatatt tcttttttaa taactgctgg aaatattctt ccactaaccc taaaccgtag
      781 gtcacatctg aactaccata aatatcaaaa cgagcttgtg tcacatccac aattaactgc
      841 tcttcctcag gcaaattctc ataatagttt tcatatattt cctcaagtat agcttctttt
      901 cgttcgattc ttcccttatc agcgtaggtt tgagaatgaa tcagctcatc tttgagtttt
      961 aggaatcctt ttggaatctc tataacctta acatccacaa tattctgtaa tgatactcca
     1021 agtttatccg caatcaataa taatttagga agtgttgcta aagcttgccc attttcaata
     1081 cgtgctagct gacgaatcgt caactctgat tcatcgctac aaatatctat ctgagttttt
     1141 ccacaagatc gcctaatagt tttgatttta taaccaattt cacgtttcaa ctcatccata
     1201 gtaatactcc ttaagttgat tacaaagatt tctttttctt cctaattctt ttgtcaatca
     1261 tatataaaac gataagcgaa aacagcggta gaacaatata actcttttca ctcattatca
     1321 gctgaaatcc cagggttgct agtatcttaa agatgaaaat attcatcaaa aatagaaaaa
     1381 caaatactaa aaaatatttt aaaaccttct tcatttcttt tcttcaactt tcctaatttc
     1441 tacacctatt ttatcgaaaa tatatataaa actccaaata aacacttcga ctgcactttt
     1501 tttggcaaaa aatagacaga acaaacatcc tacctactaa aaatatgata tgaaaggaaa
     1561 atgatttttt actgacaccc taggcaagct tgaaacaaat aaatcattga tagtaacact
     1621 tacgacatcc tatttaaagt tccttcaatt tctaatgtcc ccgctatttc taagccacct
     1681 ctggtatgat ctattcctag tcagaataga taacaccgtt ctaaattacg attcatagtt
     1741 actccttttt tgactcgtta ctataagact cctctttaca tgcgcaaaat aattttgatt
     1801 cagattcatc atttatagtt ttgattattt tttaatagat ttttctgttt tcttctccga
     1861 agactttaaa aatttttttc taattaaatc atccatataa ggaccaaata taccaactac
     1921 taaaccaata ataaaacttt taaaatccat aattaccacc aacatgtcgc tgcataggcc
     1981 acacctccaa gtatagctcc tcccgcagca ccagttgctg caccttgcca tgttcttgtt
     2041 ttaattccta gctgaagacc tcgtactgct cctcctccaa cacctgcttt ggcaaaatct
     2101 ccccaattgc atccgccacc ttcaacgcaa gcaagcatct cagtatccat aatttcaaat
     2161 tgtgacatct tttttgtatt cataacgaat acctcttttt tatttttaat atttgtcttg
     2221 tta
//
LOCUS       DAVKZB010000168         2188 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKZB010000168 DAVKZB010000000
VERSION     DAVKZB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.168"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            211..891
                     /locus_tag="VXA80_001950"
     CDS             211..891
                     /locus_tag="VXA80_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0354384.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            851..1528
                     /locus_tag="VXA80_001951"
     CDS             851..1528
                     /locus_tag="VXA80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0354385.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTISLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(1703..>2188)
                     /locus_tag="VXA80_001952"
     CDS             complement(1703..>2188)
                     /locus_tag="VXA80_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV0354386.1"
                     /translation="FCKSNTLLNFEELSKYFKKDRRIFVGGVGRSSMAARGFVNRLVH
                     LGYSAHLIGEISTPLARKDDVVLLISNSGNTNSLYTIGERAKKDGAKILSITSNKDSK
                     IFQISDDSLVLKDVDKSNQPMGSLFEQAALLVTDAFILFLMNLNIETSESMRKRHSNL
                     E"
BASE COUNT          621 a          451 c          372 g          744 t
ORIGIN      
        1 tgtcctcctt aataagtaag cgtttacttt gtatattata aaaatatctt aactcttttt
       61 caaaactttt caaatatttt gtttggaaat ttcagaaatt ttatgtctat ggtaaaaaat
      121 ccttataacg gcaataaaaa atagatatta tccaaagaag attttaagtg ctacaataac
      181 tgtattattt ctagatggga ggttctattt ttggattggt ccattgttga acaatatcta
      241 ccactatatc aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga
      301 tcctttctgc tcggtttaat cgttagtatc atccgacatt atcgaatccc tgttttggcg
      361 caagtagcga cagcctacat tgaattgtca cgtaatactc cccttttgat tcaactcttc
      421 tttctctact tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg
      481 cttgggcttg tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa
      541 gccatcagtc aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc
      601 ttttactatg tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat
      661 gtcattttcc ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg
      721 tacgtcgcca aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg
      781 gtagttgctt atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg
      841 aggacccgcc atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag
      901 aatcttacag gggttgggcg ttacgattgg gatatccatc ctgtctgtcc tcttatccat
      961 gatgttcgga acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt
     1021 aacacgattg tatctggact ttatccgtat catgccccag ctggtgctac tcttcatcgt
     1081 ttactttggc ttggctcgaa actttaatat caatatctca ggtgagactt cagctattat
     1141 cgtttttacc ctctggggaa cagctgaaat gggagacttg gtacgtggag ctatcacttc
     1201 tctccctaaa catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta
     1261 ctaccacatc atcatcccac aagtcttaag aagactgcta ccgcaggcta tcaatcttgt
     1321 cactcggatg attaaaacca tttcattagt tgttttgatt ggggttgtgg aagtgaccaa
     1381 agttggacaa caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat
     1441 ttatggaacc attctagtct tatatttcgc agtttgctac cctatttcca aactatccac
     1501 tcacttagaa aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaggaact
     1561 aaaaaaatag ggttgcccgt cgacaaccct tttaaaattt caccatagat aaagttatgt
     1621 aaatcgaata atttgtttga taaattacta taatttatta atcttaaaca acgatatatt
     1681 ctagcataag ataagatttg tatcactcca aatttgaatg tcttttcctc attgattcag
     1741 atgtttctat gtttagattc attagaaaca aaatgaaagc atcagtcaca agcaaagctg
     1801 cttgttcaaa taaactaccc ataggctgat ttgatttatc aacatccttt aataccaatg
     1861 aatcatcgct aatttgaaaa attttagaat ctttattgga tgttatactt aatattttag
     1921 ctccatcttt ttttgccctt tctcctatgg tatacaatga atttgtattt cctgaattag
     1981 atataagtaa tacaacatca tcttttctag ctaaaggagt gctaatctct cctattaaat
     2041 gtgcagagta tcctaaatga acaagtctat ttacgaatcc tcttgctgcc atacttgatc
     2101 ttccgacacc accaacgaat atccttctat ctttcttaaa atatttagat aattcttcaa
     2161 aatttaagag agtatttgat ttgcaaaa
//
LOCUS       DAVKZB010000169         2160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKZB010000169 DAVKZB010000000
VERSION     DAVKZB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.169"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            14..1237
                     /locus_tag="VXA80_001953"
     CDS             14..1237
                     /locus_tag="VXA80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEV0354387.1"
                     /translation="MLDNNIIDLLQRSCYTENRSYLSEYPSIIIYLSYRLANCDDNEH
                     SKLLYNRVNYYLHELLKSIKLNSRNNISMCYGFSGYVYALKLLPKRSKEYSKLLETLE
                     TILVSLTRDRLSEIKKSNKVKEEYIDVIQGVSSVGKYFLSKDKLTSNQELLLKGVLNY
                     LAGVINNKPTIYPEYMPNEKLKRKFPNGYINLGVAHGILGPLYVLALGFKKFNMPEYL
                     ISLKKGLSYYEKTFQTNKIGKIIGWNGRVSAEVESEKFEYNLSWCYGSLGMARVLYNI
                     SKIIDIPKLQELATDVFHSSIYYLNSSEILNNAICHGRSGIMLLFNLMYLDTGESQFK
                     AISDNLFKEIVNKATDSEYIFVERDIYFRGVNYDEVIEYIDFGLLNGVSGIVLALMAQ
                     RTGNASPLAEMFFMQ"
     gene            1266..>2160
                     /locus_tag="VXA80_001954"
     CDS             1266..>2160
                     /locus_tag="VXA80_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0354388.1"
                     /translation="MKKILNNKLYMKVLVSDLISNFGDTLYFIALMTYVTEIKSSNLA
                     ISIVNISETIPILFTIFFGIIADRTLNKVGMIIKTLWIRTILYLLVAVVMNFKESILV
                     VILASIVNLISDTLGQFENGLFYPISNRIVKKSDREETMAFRQTATSTMNIVNQSLGA
                     FLITFLSFFHLALINSLTFAISLLITLAIKSQINNFYIDKTPSTKVSKVDFKATFSDI
                     ISNLKLSLKHLFSLTNMKTVLLVIPILNGSLAIIIPLAVVNLSKSSALTIISSATTIS
                     VLGISTVSGGILGGTLILISKK"
BASE COUNT          746 a          282 c          386 g          746 t
ORIGIN      
        1 agtagatagt ctagtgttag acaataatat tattgattta ctacagcgct cgtgctatac
       61 ggaaaataga agttacttgt cggaataccc cagcataatc atatatttat catatagatt
      121 agctaattgc gatgataacg agcattctaa gttgctctac aatagagtaa actactattt
      181 acatgaactg ttaaagtcga ttaagttgaa cagtcgtaat aatatatcaa tgtgctatgg
      241 attttctggc tatgtatatg ccttgaaatt gttacctaag aggagtaaag aatatagtaa
      301 attgctagag acacttgaaa caattttagt atctttaact agagataggc taagtgaaat
      361 aaaaaaatcc aataaagtta aagaagaata tattgatgta atccaaggag tttctagtgt
      421 aggaaagtat tttcttagca aggataaatt gacttctaat caggaactat tattaaaagg
      481 agttttaaat tatttagcag gagtaataaa taataagcca actatttatc cggaatatat
      541 gccaaatgaa aaattaaaga gaaaattccc caatggctat atcaacctag gtgtagctca
      601 tggtatatta ggaccactat atgttttagc attagggttt aaaaaattca acatgccaga
      661 gtatttaatt tcgctaaaaa agggtttgag ttactatgaa aaaacttttc aaactaataa
      721 gattggcaag attattggct ggaatggtag agtaagtgct gaggtagaaa gtgagaaatt
      781 cgaatataat ttaagttggt gttatggtag tcttggaatg gcaagagtac tatataatat
      841 atctaaaata attgacatcc caaaattaca agagttagct actgatgtat tccactcatc
      901 tatttattac cttaatagta gtgaaatttt aaataacgca atttgtcatg gtcgctctgg
      961 gataatgctt ttgttcaact tgatgtattt agacacaggg gaatcacagt ttaaagctat
     1021 ttctgataat ttatttaagg aaatagtaaa taaagccact gattctgaat acatatttgt
     1081 ggaacgtgat atatatttta ggggtgtgaa ttatgatgaa gtgatagagt atattgattt
     1141 tggcttgtta aatggggttt ctgggatagt tcttgcacta atggcgcaga ggacaggaaa
     1201 tgcttctcct ttggctgaaa tgttttttat gcagtgaggg tttaaggtag atatagggat
     1261 aacatatgaa aaaaatttta aataataagc tatatatgaa agtcttggtt tcagatctga
     1321 tatcaaactt tggagataca ctatatttta tagctctcat gacttatgtg acggagatta
     1381 agagtagcaa tttggctatc tcaatcgtaa atatatcgga gactatacct atattgttca
     1441 caatattttt tggtatcatt gcagatagga cacttaataa agtgggaatg attatcaaaa
     1501 cgttatggat acggacaatt ttatatttat tagtggctgt tgtcatgaat tttaaggaat
     1561 ccattttagt agtaatactt gctagtatag ttaatcttat ttcagataca ttggggcaat
     1621 ttgaaaatgg tcttttctat ccaatttcta atcgaatagt gaaaaaatca gatagggaag
     1681 aaaccatggc gtttaggcaa acagcaactt ctaccatgaa cattgtaaat cagtcgttag
     1741 gggcattttt aatcactttt cttagtttct ttcacttagc tcttataaat tctctaacat
     1801 tcgctattag tttactaata acattagcta tcaaaagcca aattaacaat ttttatatag
     1861 acaagacccc atccactaag gtttcaaaag ttgattttaa agctacgttt tctgatatta
     1921 tttcaaactt aaaactttcg cttaaacatc tatttagtct cactaatatg aagactgtat
     1981 tacttgttat tccaattctt aatgggagct tagccattat cataccacta gctgtagtga
     2041 atctttctaa aagttctgct cttacaatta ttagttctgc aacaacaatt tcagttttgg
     2101 gaattagtac tgtttctgga gggattttag gtggaacttt gattctaatt agtaaaaaat
//
LOCUS       DAVKZB010000170         2157 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKZB010000170 DAVKZB010000000
VERSION     DAVKZB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.170"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            33..905
                     /locus_tag="VXA80_001955"
     CDS             33..905
                     /locus_tag="VXA80_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0354389.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIKMNRRNI"
     gene            905..1699
                     /locus_tag="VXA80_001956"
     CDS             905..1699
                     /locus_tag="VXA80_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354390.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT          796 a          331 c          359 g          671 t
ORIGIN      
        1 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
       61 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
      121 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
      181 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
      241 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
      301 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
      361 taatattagg tattggtttt caagctgtca caagagcagg cacctcattt ttattgttga
      421 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
      481 caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt
      541 tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg
      601 gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt
      661 tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct
      721 taaatattgg caagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt
      781 tgttatcaga catattctct caaaatattt taccagctag gtatccagta ggtgttgtaa
      841 ctggcttgtt aggttcacca tacttaatat acttactaat aaaaatgaac aggaggaata
      901 tataatgaaa ggtctgtggt caaataatct tacctgcggt tatgatgaaa aaataatctt
      961 ggaaaatata aatataaaaa tacctgaaga aaaaatatca gttattattg ggtcaaatgg
     1021 ttgtgggaaa tcaacactca ttaaaacctt gtctcgactt ataaagccat tagagggaga
     1081 agtattgctt gataataaat caattaattc ttataaagaa aaagatttag caaaacacat
     1141 agctatatta cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag
     1201 ccgtggtcgt ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat
     1261 aataaacaga tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga
     1321 actttctggg ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag
     1381 tatcctactt ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt
     1441 agacctcttg actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga
     1501 tataaatcta acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt
     1561 tgcagaggga aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct
     1621 tgaagcaaaa attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa
     1681 gcaccatgtt aactcttaat aattgaatat tggaattcat ttaaaagtcg caacttttga
     1741 actgaccctc aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatggcaa
     1801 atacagcaca gaatttaaga taaaattcgt aaaagaatat catgaatgaa aaataggata
     1861 tagagaataa cagcaatatt aaaaagatca ggactgataa taaacaaaaa gaaagtacaa
     1921 cgactagttc aaaagctaaa acttcaagta aaaagttatt caagaaaatc tagaaaatac
     1981 tcatcctaca aaggacaggt aggaaaaata tcagacaaca aaataaaaag aaatatttat
     2041 taccgaaaaa aaggggaaat cgagtcaagt accaacaggc tcatctattc caaaaagata
     2101 tttactcgat ggaaaaactc ctagaattag tgctacaaat attgacaatg gtatctt
//
LOCUS       DAVKZB010000171         2076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKZB010000171 DAVKZB010000000
VERSION     DAVKZB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.171"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            116..2008
                     /gene="pepO"
                     /locus_tag="VXA80_001957"
     CDS             116..2008
                     /gene="pepO"
                     /locus_tag="VXA80_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV0354391.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT          631 a          420 c          477 g          548 t
ORIGIN      
        1 tatcatggaa agatttttta taatagctaa aaaataatag ttaaatggtt attaattgca
       61 ttccctagtg atttttgtta agataaatgc aaatacaaat gaaagcgaga acaagatgac
      121 acgttatcaa gatgattttt atgatgctat caatggagaa tggcaacaga cagctgaaat
      181 cccagcagat aagtctcaaa caggaggttt tgttgattta gaccaggaaa ttgaagacct
      241 gatgctggcg acaacagaca agtggttagc aggtgaagaa gtgcctgagg atgctatctt
      301 ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt gacaagagag aagctgacgg
      361 tatcacacct gtcttaccac tccttaaaga attccaagaa ttggagactt ttgcggattt
      421 tacagctaaa ctagcagagt ttgagcttgc aggaaaacca aacttccttc cttttggtgt
      481 atcgccagac tttatggatg ctagaatcaa tgttctatgg gctagcgctc caagcacaat
      541 cttgccagat acgacctact atgcagaaga acatcctcag cgcgaagagc tcttgactct
      601 ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat ttctctgatg aagaaattga
      661 agacttgcta gaaaaaagac ttgaattgga ccgccgagtt gcggcagtgg tgctctctaa
      721 tgaagaaagt tcagaatatg ctaaactcta tcatccatat tcttacgaag atttcaagaa
      781 attcgcgcct gccctacctt tggatgactt cttcaaagca gttattgggc aattaccaga
      841 caaggttatt gtagacgagg aacgtttctg gcaagcagca gagcaattct acagtgagga
      901 agcctggtct ctccttaaag caaccttgat tttgagtgtt gtcaatcttt caaccagcta
      961 tttaacagag gatatccgtg ttttgtctgg tgcctacagc cgtgcccttt ctggagttcc
     1021 agaggcaaaa gataaggtca aagcagctta tcatctagca caagaacctt tcaagcaagc
     1081 cctgggtctt tggtacgccc gtgagaagtt ctctccagaa gccaaggcgg atgtggagaa
     1141 aaaagtggca accatgattg atgtttataa ggagcgtctg cttaagaatg actggctcac
     1201 tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg atcaaacctt atattggcta
     1261 tccagaagaa ttgcctgcac gttacaagga taaggtagtg aatgaaactg ccagtctttt
     1321 tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac agttggagta agtggaacca
     1381 gcctgtagat tataaggaat ggggcatgcc tgctcatatg gtcaatgcct actacaatcc
     1441 tcagaagaac ctgattgtct ttccagcggc cattttacag gcgcctttct atgacttgca
     1501 tcagtcatct tctgctaact acggtggtat tggggcagtg attgcccatg aaatttccca
     1561 cgcctttgat actaacgggg cttcctttga cgaaaatggt agcctcaagg attggtggac
     1621 agagagcgac tatgctgcct tcaaggagaa aacacaaaaa gtcattgacc aatttgatgg
     1681 acaggattct tatggagcaa ccattaacgg taaattgact gtatcagaaa acgtggctga
     1741 cttgggagga atcgcagcag cgcttgaagc agctaagaga gaagcagact tctcagcaga
     1801 agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa ggtcgaccag aatttatgaa
     1861 acttttggct agcgtcgatg tgcacgcacc agccaaactc cgtgtcaatg tgcaagtacc
     1921 aaacttcgac gatttcttta caacctatga tgtcaaagaa ggagacggaa tgtggcgttc
     1981 accagaggag cgcgtgatta tttggtaatg caaaaaatct agtcatcaga aaaaaggcat
     2041 ccaatcatgt gtgaaaacct tgataggatg cctttt
//
LOCUS       DAVKZB010000172         1995 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKZB010000172 DAVKZB010000000
VERSION     DAVKZB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.172"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            102..1283
                     /locus_tag="VXA80_001958"
     CDS             102..1283
                     /locus_tag="VXA80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV0354392.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1341..>1995
                     /locus_tag="VXA80_001959"
     CDS             1341..>1995
                     /locus_tag="VXA80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV0354393.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     "
BASE COUNT          722 a          353 c          366 g          554 t
ORIGIN      
        1 ttcaaaagag ttatccacag tttgtgtaaa acttttgtgt ttaagttata attaagctag
       61 tcagtttata ctttcagtaa ttcaaacata tggaggcaaa tatgaaacat ctaaaaacat
      121 tttacaaaaa atggtttcaa ttattagtcg ttatcgtcat tagctttttt agtggagcct
      181 tgggtagttt ttcaataact caactaactc aaaaaagtag tgtaaacaac tctaacaaca
      241 atagtactat tacacaaact gcctataaga acgaaaattc aacaacacag gctgttaaca
      301 aagtaaaaga tgctgttgtt tctgttatta cttattcggc aaacagacaa aatagcgtat
      361 ttggcaatga tgatactgac acagattctc agcgaatctc tagtgaagga tctggagtta
      421 tttataaaaa gaatgataaa gaagcttaca tcgtcaccaa caatcacgtt ataaatggcg
      481 ccagcaaagt agatattcga ttgtcagatg ggactaaagt acctggagaa attgtcggag
      541 ctgacacttt ctctgatatt gctgtcgtca aaatctcttc agaaaaagtg acaacagtag
      601 ctgagtttgg tgattctagt aagttaactg taggagaaac tgctattgcc atcggtagcc
      661 cgttaggttc tgaatatgca aatactgtca ctcaaggtat cgtatccagt ctcaatagaa
      721 atgtatcctt aaaatcggaa gatggacaag ctatttctac aaaagccatc caaactgata
      781 ctgctattaa cccaggtaac tctggcggcc cactgatcaa tattcaaggg caggttatcg
      841 gaattacctc aagtaaaatt gctacaaatg gaggaacatc tgtagaaggt cttggtttcg
      901 caattcctgc aaatgatgct atcaatatta ttgaacagtt agaaaaaaac ggaaaagtga
      961 cgcgtccagc tttgggaatc cagatggtta atttatctaa tgtgagtaca agcgacatca
     1021 gaagactcaa tattccaagt aatgttacat ctggtgtaat tgttcgttcg gtacaaagta
     1081 atatgcctgc caatggtcac cttgaaaaat acgatgtaat tacaaaagta gatgacaaag
     1141 agattgcttc atcaacagac ttacaaagtg ctctttacaa ccattctatc ggagacacca
     1201 ttaagataac ctactatcgt aacgggaaag aagaaactac ctctatcaaa cttaacaaga
     1261 gttcaggtga tttagaatct taattgacat ctatgtaaag aaagctttac ataagagaaa
     1321 agatgtgtta gtgtagaatc atggaaaaat ttgaaatgat ttctatcaca gatatacaaa
     1381 aaaatcccta tcaaccccga aaagaatttg atagagaaaa actagatgaa ctagcacagt
     1441 ctatcaaaga aaatggggtc attcaaccga ttattgttcg tcaatctcct gttattggtt
     1501 atgaaatcct tgcaggagag agacgctatc gggcttcact tttagctggt ctacggtcta
     1561 tcccagctgt tgttaaacag atttcagacc aagagatgat ggtccagtcc attattgaaa
     1621 atttacagag agaaaattta aacccaatag aagaagcacg cgcctatgaa tctctcgtag
     1681 agaaaggatt cacccatgct gaaattgcag ataagatggg caagtctcgt ccatatatca
     1741 gcaactccat tcgtttactt tccttgccag aacagattct ctcagaagta gaaaatggca
     1801 aactatcaca agcccatgcg cgttccctag ttgggttaaa taaggaacaa caagactatt
     1861 tctttcaacg gattatagaa gaagatattt ctgtaaggaa gttagaagct cttctgacag
     1921 agaaaaaaca aaagaaacag caaaaaacta attatttcat acaaaatgaa gaaaaacagt
     1981 taagaaaact actcg
//
LOCUS       DAVKZB010000173         1989 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKZB010000173 DAVKZB010000000
VERSION     DAVKZB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.173"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            18..1868
                     /locus_tag="VXA80_001960"
     CDS             18..1868
                     /locus_tag="VXA80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0354394.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
BASE COUNT          542 a          380 c          451 g          616 t
ORIGIN      
        1 aggaggagta gatttctatg aataaaaaac taacagatta tgtgattgat ctggtggaaa
       61 ttttaaataa acaacaaaag caggttttct ggggaatatt tgatattttc agtatggtgg
      121 tttccatcat tgtatcttat attttatttt atgggctgat taatccagca cctgttgact
      181 acattatcta tacgagtttg gccttcctgt tctatcaatt gatgattggt ttttgggggt
      241 tgaacgcgag cattagtcgt tacagcaaga ttacggattt catgaaaatc ttttttggtg
      301 tgactgctag cagtgtcttg tcatatagta tctgttatgc cttcttgcca ctcttctcca
      361 tccgtttcat cattctcttt atcttgttga gtaccttctt gattttattg ccacggatta
      421 cttggcagtt aatctactcc agacgcaaaa aaggtagtgg tgatggagaa caccgtcgga
      481 ccttcttgat tggtgccggt gatggtgggg ctctttttat ggatagttac caacatccaa
      541 ccagtgaatt agaactggtc ggtattttgg ataaggattc taagaaaaag ggtcaaaaac
      601 ttggtggtat tcctgttttg ggctcttatg acaatctgcc tgaattagcc aaacgccatc
      661 aaatcgagcg tgtcatcgtt gcgattccgt cgctggatcc gtcagaatat gagcgtatct
      721 tgcagatgtg taataagctg ggtgtcaaat gttacaagat gcctaaggtt gaaactgttg
      781 ttcagggcct tcaccaagca ggtactggct tccaaaaaat tgatattacg gaccttttgg
      841 gtcgtcagga aatccgtctt gacgaatcgc gtctgggtgc agaactgaca ggtaagacca
      901 tcttagtcac aggagctgga ggttcaatcg gttctgaaat ctgtcgtcaa gttagtcgct
      961 tcaatcctga acgcattgtc ttgctcggtc atggggaaaa ctcaatctac cttgtttatc
     1021 atgaattgat tcgtaagttc caagggattg attatgtacc tgtgattgcg gacattcaag
     1081 actatgatcg tttgttgcaa gtctttgagc agtacaaacc tgctattgtt tatcatgcgg
     1141 cagcccacaa gcatgttcct atgatggagc gcaatccaaa agaagccttc aaaaacaata
     1201 tccgtggaac ttacaatgtt gctaaggctg ttgatgaagc taaagtgtct aagatggtta
     1261 tgatttcgac agataaggca gtcaatccac caaatgttat gggagcaacc aagcgcgtgg
     1321 cggagttgat tgtcactggc tttaaccaac gtagccaatc aacctactgt gcagttcgtt
     1381 ttgggaatgt tcttggtagc cgtggtagtg tcattccagt ctttgaacgt cagattgctg
     1441 aaggtgggcc tgtaacggtg acagacttcc gcatgacccg ttattttatg accattccag
     1501 aagctagccg tctggttatc catgctggtg cttatgccaa agatggggaa gtctttatcc
     1561 ttgatatggg caaaccagtc aagatttatg acttggccaa gaagatggtg cttctaagtg
     1621 gccacactga aagtgaaatt ccaatcgttg aagttggaat ccgcccaggt gaaaaactct
     1681 acgaagaact cttggtatca accgaactcg ttgataatca agttatggat aagattttcg
     1741 ttggtaaggt taatgtcatg cctttagaat ccatcaatca aaagattgga gagttccgca
     1801 ctctcagtgg agatgagttg aagcaagcta ttatcgcctt tgctaatcaa acaacccaca
     1861 ttgaataaaa aagaaaaacg catagtatca agttacacaa ccttggtaat atgcgtttta
     1921 ttatgtagag acttatactc ttcgaaaatc tcttcaaacc acgtcaacgt cgccttgccg
     1981 tatatggtt
//
LOCUS       DAVKZB010000174         1886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKZB010000174 DAVKZB010000000
VERSION     DAVKZB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.174"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(129..1265)
                     /gene="lctO"
                     /locus_tag="VXA80_001961"
     CDS             complement(129..1265)
                     /gene="lctO"
                     /locus_tag="VXA80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV0354395.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT          571 a          415 c          330 g          570 t
ORIGIN      
        1 gaaatcgatt taaatgtcct gattgatttg ttctgttttt attccatttt actatagttt
       61 tcgatagcga tttattcttc caatacacga agaaaaacct ccacattcag tggaggcaat
      121 ctgttttatc aatacaattt taagtcacga gggtcaactg ggaaggttgg gttgtatgga
      181 ttgtgacgga gcttaaagtg tttgacatct tcaatggtct gagctccaga caattgcata
      241 actgtcttca attccgcatt caagtgttca aagacttgac gcacaccgac actaccaccg
      301 agagccaagc cataaataac agggcgacca atagctacca agtctgctcc tgaagccaag
      361 gctttaaaga cgtgttgacc acgacgaaca ccagaatcaa agacaatcgg cacacgtcta
      421 tcaactgctt ctgccacttc ttgaagcgag tcaaaggcag ctggtccacc gtcgatttga
      481 cgaccaccgt ggttggttac ccagatacca gaagctcctg cagcaagcga acgttcaacg
      541 tcctcacggc attgtggtcc cttgacatac acaggaagac cagagtattc agcgataaat
      601 tctacatcgc gtggagacaa gcgttgttta gctgatttgt aaacaaagtc cattgattta
      661 ccagcacctt ctggcaggta ttcttcaaca atcggcatgc caactgggaa gacaaaacca
      721 ttacgcttat ccacttcacg attcccccct acagtagcat ctgccgtcaa gacaatcgct
      781 ttataacctt cagccttcac acggtccatg atgtggcggt tgataccgtc atccttacta
      841 aagtaaaatt gaaaccaatg aggtgtccct tgaagggctt cagaaatctc tggaaggtca
      901 acagtagagt aagaactggt tgtataaaga gaaccaaact catgcacacc acgcgcagtc
      961 gccacttccc cctgttcatt tgccaattta tgagccgcaa caggtgccat aatgattggc
     1021 gaagatagtt tttcacctgc aaattcaatc tctgtacttg gattttcaac atcgcaaagt
     1081 gtatgaggaa cgatgagttt gtggttaaag gcgcggatat tctctcttaa agtgaaagta
     1141 tcttccgccc cactagcgat atagccaaat gctgctttag gaataacttg ttgcgccatt
     1201 ggctccaaat cataggtatt gatgaaatct acatgacctt ctgcattgct tgttttgtat
     1261 gacataaact gtcccccttg attaagtaag cgtttgcttt ttactataca actataataa
     1321 ctcttttttc aatcattttc aagagttttg cttaaaaacc atcttatttt ttcatacaat
     1381 atactagccc tactttcctt aaactctaaa ctttagtcta tattaagtaa atcgttgaga
     1441 ttttttgctg ccaggcattt gaactacttt tttatttgct gattctatct actaatacta
     1501 taaaaaaaca agtgaataag acaccagaac atcctctaat aggacctatg gacttatatc
     1561 agtcccatat atactgttta agcaagatac tatcctagtt gacgctaact tcctatccca
     1621 cttattaatt ttttatttat agtgaatctg aacaaatcaa ttggggaatc caagtcaatt
     1681 tctagtactt tttgaacagt ttcagcgtac tatcgtaaaa tgaaataaaa catgcacaaa
     1741 ttgcttaggg aatttaaacc aatttctaag aatgtcttag aaatcaaagt gtactatttc
     1801 aacttcaata tactacactt cattttctcc ttaaattcag tctctttttg aatcaaaaaa
     1861 tcctctggta agagccagag gataaa
//
LOCUS       DAVKZB010000175         1801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKZB010000175 DAVKZB010000000
VERSION     DAVKZB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.175"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            19..936
                     /gene="adcAII"
                     /locus_tag="VXA80_001962"
     CDS             19..936
                     /gene="adcAII"
                     /locus_tag="VXA80_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV0354396.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            944..>1801
                     /gene="phtB"
                     /locus_tag="VXA80_001963"
     CDS             944..>1801
                     /gene="phtB"
                     /locus_tag="VXA80_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV0354397.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT          602 a          331 c          386 g          482 t
ORIGIN      
        1 atggttaagg agttgttcat gaagaaacaa aatttatttt tagtcctgtt aagtgtcttt
       61 cttttgtgct tgggggcttg tggtcaaaag gaaagtcaga caggaaaggg gatgaaaatt
      121 gtgaccagtt tttatcctat ctacgctatg gttaaggaag tatctggtga cttgaatgat
      181 gttcggatga ttcagtcaag tagtggtatt cactcctttg aaccttcggc aaatgatatc
      241 gcagccatct atgatgcaga tgtctttgtt taccattctc atacactcga atcttgggca
      301 ggaagtctgg atccaaatct aaaaaaatcc aaagtgaagg tcttagaggc ttctgaggga
      361 atgaccttgg aacgtgtccc tggactagag gatgtggaag caggggatgg agttgatgaa
      421 aaaacgctct atgaccctca cacatggcta gatcctgaaa aagctggaga agaagcccaa
      481 attatcgctg ataaactttc agaggtggat agtgagcata aagagactta tcaaaaaaat
      541 gcgcaagcct ttatcaaaaa agctcaggaa ttgactaaga aattccaacc aaaatttgaa
      601 aaagcgactc agaaaacatt tgtaacacaa catacagcct tttcttatct agcgaagaga
      661 tttgggctta atcaacttgg tattgcaggt atctctcctg aacaagaacc aagtccacga
      721 caactaacag aaattcagga atttgttaag acctataagg ttaaaacgat ttttacagaa
      781 agtaacgctt cttcaaaagt agctgaaact cttgtcaaat caacaggtgt gggtcttaaa
      841 actctgaatc ctttagagtc agacccacaa aatgacaaga cctatttaga aaatcttgaa
      901 gaaaatatga gtattctagc agaagaatta aagtgaggaa agaatgaaaa ttaataaaaa
      961 atatctagca ggttcagtgg cagtccttgc cctaagtgtt tgttcctatg agcttggacg
     1021 ttaccaagct ggtcaggata agaaagagtc taatcgagtt gcttatatag atggtgatca
     1081 ggctggtcaa aaggcagaaa acttgacacc agatgaagtc agtaagaggg aggggatcaa
     1141 cgccgaacaa atcgtcatca agattacgga tcaaggttat gtgacctctc atggagacca
     1201 ttatcattac tataatggca aggtccctta tgatgccatc atcagtgaag agctcctcat
     1261 gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca agggtggcta
     1321 tgtcattaag gtaaacggta aatactatgt ttaccttaag gatgcagctc atgcggataa
     1381 tattcggaca aaagaagaga ttaaacgtca gaagcaggaa cgcagtcata atcataactc
     1441 aagagcagat aatgctgttg ctgcagccag agcccaagga cgctacacca cagatgatgg
     1501 ttatatcttt aatgcttctg atatcattga agatactggt gatgcctata tcgttcctca
     1561 tggcaatcac ttccactata ttcctaagag tgatttatct gccagtgaat tagctgctgc
     1621 ccaagcctat tggaatggga agcagggatc tcgtccttct tcaagttcta gtcataatgc
     1681 aaatccagct caaccaagat tgtcagagaa ccacaatctg actgtcactc caacttatca
     1741 tcaaaatcaa ggggaaaaca tttcaagcct tttacgtgaa ttgtatgcta aacccttatc
     1801 a
//
LOCUS       DAVKZB010000176         1799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKZB010000176 DAVKZB010000000
VERSION     DAVKZB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.176"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            15..152
                     /locus_tag="VXA80_001964"
     CDS             15..152
                     /locus_tag="VXA80_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV0354398.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDEMRFSTFSTNHCGNWSAFSWEN
                     C"
     gene            447..1798
                     /locus_tag="VXA80_001965"
     CDS             join(447..888,888..1798)
                     /locus_tag="VXA80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV0354399.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMIELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
BASE COUNT          624 a          279 c          400 g          496 t
ORIGIN      
        1 aggaggagct agctatggca gaagaagtat taaatcttca attagtatct gttcaagttg
       61 acgaaacaga tgaagttgat gaaatgcgat ttagtacatt cagtacaaac cattgcggta
      121 actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac tttagggatt
      181 ctctggagtt atggatgtcg ttccaatatg tgtacgttgg aatgttagtg cttatatggg
      241 ggaacagaat cctctcttga ttgaagacaa gttagccatt aggctggttt gtctctttgt
      301 caactgtagt gggttgatat aatagtatta gtgagtggga taaaagtttc atttagttta
      361 ttcagtacaa atttaacggg tcaagatttt atgaactgca ccccaaaagt tagacagaaa
      421 aaatctaact tttggggtgt tttattatga aattgagtta tgaagataaa gttcagatct
      481 atgaactaag aaagcaagga caaagcttca aacagctttc aaaaagattt ggtgtggatg
      541 tttctggtct aaagtacatg gtgaaattaa ttgaccgtta tggcatagaa atcgttaaaa
      601 aagggaagaa tcgtcactat tcttctaaat tgaaacaaga aatgatggat aaagccttac
      661 tggaaggttg ttcacaaaga agtattagtc ttgattatgc cctcccaaac caaggaatgc
      721 tttcattttg gccggcacaa tacaagaaaa acgggtatac tattgttgag aaaacaagag
      781 ggagaccagc taaaatggga cgtaaacgga agaagacttg ggaagaaatg atagaactag
      841 aacgactcca ggaggagaat gaacgcttac ggactgaggt ggcctaccta aaaaagttaa
      901 aagagttaga ggaaagggac gaagccctag agcgagaaag gcagagacag ttagagaaat
      961 ggtttcagga ggatttcgac tagattttct tcttgaaaca gcacgtttag ctcgctcgac
     1021 ttactattat cagttgaaac aactggatgg ggttgataaa gataaagaga ttaaaaccga
     1081 aattcagggc attgataatg aacataaagg caactatggc tatcgtcgaa tccatttaga
     1141 actaaggaat cgtggttttg tggtcaatca taagaaagtt caacggttga tgagaattct
     1201 tggtttaacg gctcgaattc gccgtaaacg gaagtattct tcctaccaag gagagattgg
     1261 caagaaagca gagaatctca ttcaacgcca gttcgaagca tcgaggccaa tggaaaagtg
     1321 ctatacagat gtgacagagt ttgccattcc aaatagcaca cagaaactct atttatcacc
     1381 agttttagat ggttttaaca gtgaaatcat tgcttatcat ctttctactt cgccaaactt
     1441 agaacaagtg aagagtatgt tagaacaggc ctttacagag aaatactatg agaatacgat
     1501 tctccatagt gaccaaggct ggcaatacca acacgattct tatcatcggt tcctagagag
     1561 taagggaatt caagcatcca tgtcacgcaa gggcaacagc ccagacaaca gcatgatgga
     1621 atctttcttt ggcattttaa aatccgaaat gttttatggc tatgagaaga actttagatc
     1681 tttagaaaac cttgaacaag ctattgtgga ctacattgat tactacaaca acaaacgaat
     1741 taaggtaaaa ctaaaaggac ttagttctgt gcagtacaga actaaatcct ttggataaa
//
LOCUS       DAVKZB010000177         1792 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKZB010000177 DAVKZB010000000
VERSION     DAVKZB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.177"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            520..1383
                     /locus_tag="VXA80_001966"
     CDS             520..1383
                     /locus_tag="VXA80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0354400.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQGQMAETYRRLNAIVIKNALHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKSGFLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGNNQKEIENFIQSLQVLENPQLTSLFE
                     LRLQQYKALID"
     gene            1675..1779
                     /locus_tag="VXA80_001967"
     CDS             1675..1779
                     /locus_tag="VXA80_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0354401.1"
                     /translation="MENTEFSLELDVTEVATEQDYVSSGVTSTGCCKN"
BASE COUNT          601 a          290 c          347 g          554 t
ORIGIN      
        1 tttttgaaaa gaattttgaa ctatttacaa aacaaagcga tctaattgtt gcaaatagat
       61 gggatagggg ccttgaagct tataaagata aagtttacac aagggggatc tggataagag
      121 attaactatt gatattaaaa aggcaataat ttataaaatt tgtacaaaaa aatgttaaat
      181 tttcacattt tcatagtata ctgtttaata gaaagtaggt tctttgtaac cgctccatta
      241 taaaatcctt tcactcttcc ggtatactat taggaaaaac ataactggag gatattttga
      301 gctcacacct catcgttcca gtggctgttc cccaagctcg tgtactgccc aaaaaagtta
      361 gacattctat ttagaacgag gattgagttc tgtattgtac aaggctcggt ccttttagag
      421 tcagcttaag gctggctttt ttaatcacca aagtgtcaga atgttttgac aaatgaacac
      481 aaataatgat ataatatgca aaagctagga ggtggtagga tgattgaaaa aatggaactg
      541 ggggaatttt acaaggaatt gcgtttggcg agaaagctca agcagtcaga tgtggcttgt
      601 gctggactaa cagcttctca gctatccaag ttcgaactag ggcagtctat gctgtctgcg
      661 gataagctca tactagccat ccaagggatc aatgtgacct ttgatgagtt tgggcacaag
      721 ctcaacaact atcaagaatc tccacatatg cagattggta gaaaggttgt ggatcgcttt
      781 gctcatcaag atatagctgg cttagagcaa ctgttggagg aagtcgagca aggacagatg
      841 gcggagacct atcgtcgttt gaatgctatt gtgattaaaa atgcactcca ttccttagat
      901 aaaagctatc cgctagcaga ggaggatagc gagtttttga ctacctacct ctatgctatt
      961 gagtcttgga cctggtttga actctatctt ttttgcaata ccatgccctt cttgagcaat
     1021 caagatctga tctttttatc aacctctttg cttgaaaaat ccaaggaatt taaagagttg
     1081 gtacataata gactgtatat gaaaagtggc tttctgaata ttatatcaga gctcatggag
     1141 cgcaagcttt tctcctatat cccaatcttt gaagcagagc tggagagcat gttgcgtccg
     1201 tacgatgttt ttgaaaaagt attgtggcaa tttttaaaga aaatgagtat tttccttcaa
     1261 accaaaggaa acaatcaaaa agagattgaa aattttatcc aatctctgca agtattagaa
     1321 aatccacaat taacatccct ttttgaacta cgcttacagc aatacaaagc acttatcgat
     1381 tagttagaaa tgggaaaaag ataataatct cagcaacgat tgtcaataat gttaaaactt
     1441 gtttcttcat atgacttcca ctcctcatta gtaataactt tattatacca gggaaatgat
     1501 caaatctact aaaatcgcaa atgtgaaatt tttataagaa aaaatatcaa atatgcgatt
     1561 ttctaaaata agccaatttt cgtgttatac tgtacttact aaagcacttg aagcaaatcc
     1621 taggtcgcag aagtggttta taagtaaaaa taattggagg aggaatcgtt tattatggaa
     1681 aatacagaat tttcattgga acttgatgtg actgaagttg caactgaaca agattatgtt
     1741 tctagtggtg ttacatctac aggttgttgt aaaaactaat ttaataattg at
//
LOCUS       DAVKZB010000178         1717 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKZB010000178 DAVKZB010000000
VERSION     DAVKZB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.178"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            155..562
                     /locus_tag="VXA80_001968"
     CDS             155..562
                     /locus_tag="VXA80_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0354402.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTTEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            727..1026
                     /locus_tag="VXA80_001969"
     CDS             727..1026
                     /locus_tag="VXA80_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV0354403.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1287..1610
                     /gene="tnpB"
                     /locus_tag="VXA80_001970"
     CDS             1287..1610
                     /gene="tnpB"
                     /locus_tag="VXA80_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0354404.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          583 a          337 c          295 g          502 t
ORIGIN      
        1 tttttacgaa aatctattga atatgccata agaagattat accacattgt gtactatttt
       61 tggttcattt cactataata ataacgatac tagataatga ggaaaatgca atggcaaacc
      121 caactttcgg agaaaaaaag cgaatacaga ctatattgct cgctatggcg tatatgcagt
      181 tatccctaat cctgaacaaa aacaaattgt tcttgttcaa gaaccaaatg gtgcttggtt
      241 cctaccatgt ggagaaattg aagcaggtga aaatcatcag gaagccctaa agcgtgagtt
      301 gattgaagag cttggtttca caacagaaat tggtacctat tacggacaag ctgacgaata
      361 tttctattct cgtcatcgtg atacctacta ctacaatcct gcctacctct atgaagcaac
      421 tcctttcaaa gaagtacaaa agccactaga aaactttaat catattgcct ggttccctat
      481 tgacgaggct atcaaaaacc ttaaacgtgg tagccataaa tgggccattg aatcttggaa
      541 aaaacagcat aagattgact aatactcttc gaaaataaaa ttcaaaccac atcagcgtcg
      601 ccttaccata ctcaagtata gcctgcggct agcttcctag tttgctcttt tattttcatt
      661 gagtataaat caagcgactt tattcaaaaa gtgctataat agaattagaa aatcggagga
      721 aatattatga agcttatcaa tacgacaaac tcacactcgc aacttgtaaa aagtcagcta
      781 gaaagtaccg atgcaaccct tgttgaggtt tattctgcag ggaatacaga tgttattttt
      841 acccaagctc cgcttcacta tgaaatcctc atctcaaaca aacaccgtgc tattcgtgag
      901 cctgaaatcg aaaccattca agaatttttc ttgaaacgta aaattgataa ggcctctgtt
      961 gatgaagcca atatcaagac actctactca gagaaattaa ttggaatttc aatcccaatc
     1021 aagtaagaaa attgagtaaa aactcgattt gagagaaagt ccaataagtc tttccataat
     1081 aaaacgcata gtagcaagag tttctacacc cactattata cgtttttatc attttgaaat
     1141 ctgctgctaa tcaatgaaga agcaaagatc aaactaggca gctagttaca agttacgtaa
     1201 gcgcgtcata acaaggtatc tatcctaatt ccccaacttc cagtatacta gataaaaaat
     1261 aattatcgga ggaaaagtat tgtactatga tgattcaact aagtgattta ggtcaagttc
     1321 accttgtttg tggcaagaca gatatgatac aaggaatcga ttcactggct tatctcgtta
     1381 aaacccactt tgaattggat cctttctccg gtcaagtctt tctcttttgt ggtggacgta
     1441 aagaccgctt taaagccctt tactgggatg gtcaaggatt ttggatacta tataaacgct
     1501 ttgagaacgg aaaactgact tggcccagta cagaaaagga tgtcaaagct ctcgcacctg
     1561 aacaagtaga ctggcttatg aagggctttt ctatcactcc ccaaatatag tggatttaaa
     1621 ctagaatagt acacccctac ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc
     1681 aatttgtcct gttcttgttt cattttacta tagaacg
//
LOCUS       DAVKZB010000179         1677 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKZB010000179 DAVKZB010000000
VERSION     DAVKZB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.179"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            187..1677
                     /gene="lysS"
                     /locus_tag="VXA80_001971"
     CDS             187..1677
                     /gene="lysS"
                     /locus_tag="VXA80_001971"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV0354405.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIVQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT          506 a          367 c          354 g          450 t
ORIGIN      
        1 aagcagcatt tggctatatc gctagtgggg cggaagatac tttcacttct ttccagtgat
       61 tttagcgtca ggttcttttt agtttttaaa gattatccgt gaatttcttg cttatttatg
      121 ataaaatggg agtatcgcaa aaaatgactc atcgtattca attttgagta aaactaggag
      181 gatcccatgt ctacagaaca tatggaagaa ctaaatgacc agcagatcgt tcgccgtgaa
      241 aaaatggctg cgctccgcga acaaggaatc gatcctttcg gaaaacgttt tgaacgtact
      301 gcaaattcac aagaattaaa agataaatat gccaacctcg ataaagaaca attacacgat
      361 aaaaacgaaa cagctactat cgcaggacgc ttggtaacca aacgtggtaa aggtaaagtg
      421 ggttttgccc accttcaaga ccgcgaaggc cagattcaga tctatgttcg taaggatgct
      481 gtcggtgaag aaaactacga aatcttcaaa aaagcagacc ttggtgactt ccttggtgtc
      541 gaaggtgaag tgatgcgtac ggatatggga gaactctcta tcaaggcaac ccacatcaca
      601 cacttgtcta aggctcttcg tcctcttcct gagaaattcc atggtttgac agacgttgaa
      661 acaatttacc gtaaacgtta ccttgacttg atttctaacc gtgaaagctt tgaacgcttt
      721 gtcactcgtt caaaaatcat ctctgaaatc cgtcgttacc ttgaccaaaa aggattcctt
      781 gaagtggaaa cacctgttct tcataatgaa gccggtggtg ctgctgcccg tccatttatc
      841 acccaccaca atgcccaaaa cattgacatg gtgcttcgta tcgcgactga gcttcactta
      901 aaacgcctta tcgtgggtgg tatggaacgt gtctatgaaa ttggccgtat cttccgtaac
      961 gagggaatgg acgctactca taaccctgag tttacttcta tcgaagttta ccaagcttat
     1021 gcagacttcc aagacatcat ggacttgacg gaaggcattg tccaacacgc tgctaaatca
     1081 gttaagggcg atggcccagt caactaccaa ggtactgaaa tcaagattaa cgaaccattt
     1141 aagcgtgttc atatggtgga tgctatcaga gaaattactg gtgtcgattt ctggcaagac
     1201 atgactttgg aagaagctaa agctatcgct gctgagaaga aagttccagt tgagaaacac
     1261 tacactgagg ttggtcacat catcaatgcc ttctttgaag agtttgttga agaaacttta
     1321 atccaaccaa cctttgtcta tggacatcca gtagctgtat ctccactcgc taagaaaaat
     1381 cctgaagacc aacgctttac tgaccgtttc gagctcttta tcatgactaa ggagtacggt
     1441 aatgccttta ctgagttgaa cgacccaatc gaccaactta gccgttttga agcccaagct
     1501 aaagccaaag aacttggtga tgatgaagcg acaggaatcg actatgacta catcgaggct
     1561 cttgaatacg gtatgccacc aacaggtggt ttgggaatcg gtatcgaccg tctctgcatg
     1621 ctcctcactg atacaacaac tatccgtgat gtattgctct tcccaacaat gaaataa
//
LOCUS       DAVKZB010000180         1663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKZB010000180 DAVKZB010000000
VERSION     DAVKZB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.180"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            215..1576
                     /gene="dnaA"
                     /locus_tag="VXA80_001972"
     CDS             215..1576
                     /gene="dnaA"
                     /locus_tag="VXA80_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV0354406.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT          568 a          285 c          292 g          518 t
ORIGIN      
        1 aaggctgttc ttttattttt ttatctcaca aggttatcca ctatgttttt cgataaaaag
       61 cttaataaat caataatttc ttcttttatc cccaacctgt ggataaagtt tagtaacatt
      121 gtggattatt tttcacagct tgtggaaaat tcttgctatc tatggtaaaa tatctctagt
      181 attaaacttt taaatagtaa aggaggagaa aggattgaaa gaaaaacaat tttggaatcg
      241 tatattagaa tttgcacaag aaagactgac tcgatccatg tatgatttct atgctattca
      301 agctgaactc atcaaggtag aggaaaatgt tgccactata tttctacctc gctctgaaat
      361 ggaaatggtc tgggaaaaac aactaaaaga tattattgta gtagctggtt ttgaaattta
      421 tgacgctgaa ataactcccc actatatttt caccaaacct caagatacga ctagctcaca
      481 agttgaagaa gctacaaatt taactcttta taactatagt ccaaagttag tatctattcc
      541 ttattcagat acgggattaa aagaaaagta tacctttgat aactttattc aaggggatgg
      601 aaatgtttgg gctgtgtcag ccgctttagc tgtctctgaa gatttggctc tgacctataa
      661 ccctcttttt atctatggag gaccaggcct tggtaagact cacttattaa acgctattgg
      721 aaatgaaatt ctaaaaaata ttcctaatgc gcgtgttaaa tatatccctg ccgaaagctt
      781 tattaatgac tttcttgatc acctaagact tggggaaatg gaaaagttta aaaagaccta
      841 tcgtagtctt gatcttttgt taatcgatga tatccagtca ctcagcggaa aaaaagttgc
      901 aactcaggaa gaatttttca atacctttaa cgcccttcat gacaagcaaa aacagattgt
      961 cctaacgagt gatcgtagtc caaaacatct agaagggctc gaggagaggc ttgtcacgcg
     1021 ttttagttgg ggattgacac aaactatcac accccctgac tttgaaacac gtattgccat
     1081 tttacaaagt aaaacggaac atttaggcta caatttccaa agtgatactc tagaatacct
     1141 agctgggcaa tttgattcaa atgttcgaga tcttgaggga gccatcaacg acatcacttt
     1201 aattgccaga gtaaaaaaaa tcaaggatat cactattgat attgctgcag aagccattag
     1261 agcccgcaaa caagatgtta gccaaatgct cgtcatccca attgataaaa tccaaactga
     1321 agttggtaac ttttatggtg ttagtatcaa agaaatgaag ggaagtagac gccttcaaaa
     1381 tattgttttg gcccgtcaag tagccatgta tttatctaga gaactaacag ataatagtct
     1441 tccaaaaatt gggaaggaat ttgggggaaa agatcatacc acagtcattc atgcccatgc
     1501 caaaataaaa tctttgattg atcaagacga taatttacgt ttagaaattg aatcaatcaa
     1561 aaagaaaatc aaataatttg tggataactt ttagtttttt atctttttta tccacatttt
     1621 ttaaacaagc taaaaaactt gatatgactt gtttaaaggc tgt
//
LOCUS       DAVKZB010000181         1633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKZB010000181 DAVKZB010000000
VERSION     DAVKZB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.181"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            3..689
                     /locus_tag="VXA80_001973"
     CDS             3..689
                     /locus_tag="VXA80_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0354407.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            690..1622
                     /locus_tag="VXA80_001974"
     CDS             690..1622
                     /locus_tag="VXA80_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0354408.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
BASE COUNT          527 a          223 c          326 g          557 t
ORIGIN      
        1 gaatggaaac taaaaaaatt aaaaatttaa aaggtcaaat aattgtttcc tgccaagcgt
       61 tagaaggaga gccactttat actccaaatg gaggagtgat gccgttatta gcaaaagccg
      121 cctttcaagc aggcgctaaa ggtattcgtg ctaattctgt ccgcgatatt tcagaaataa
      181 aagaagaagt tgacttacca attataggta tcataaaaag agattacgat ggctttgaac
      241 cttttatttc agcaactatg aaagagattg atgaattagt tagtgaagga gtagacattt
      301 tagctttgga ttgtactaat cgttcaagac caggttatga taatattact gactttattc
      361 atgatattaa agtcaagtat ccaaaccaat tattaatggc tgatatttct acttttgaag
      421 aagggaaagt agctgctgaa tcgggagtag attttgtggg tacgacatta tctggttata
      481 caccatatag ccctaaaaaa gataatcctg attttgagtt agttgaaaga ttggtaaaag
      541 agcttgacgt accagttatt gctgaaggaa gaataagtac tccggaacaa gctagaaaaa
      601 tgttagattt aggtgcttac gcagtggttg ttggaggagc tatcactcgt cctttggaaa
      661 tagcgaagaa gtttattgag gtagtttaga tgaatcaaga aatattaaaa aagagtaaga
      721 ttcaattgac agttgaacat attcaaaaat attggatttt atatttaatg atgattccag
      781 caactgttct actaatttta tttacgtatg gaccaatgta cggcattatt atggcctttc
      841 aagactttac tgtcttcaag ggatatactg gaagtccatt tgtcggttta aaacattttc
      901 aaagattgtt ctcagatcct ttgttttata gattgttcaa gaatacgttc atggtagggg
      961 ttttagactt tttattctct ttcccagctc cgcttatttt tgcactcata ttaaacgaag
     1021 tcagaaaagt aagatttaag agtgtagttc aatctattag ttatcttcct cattttattc
     1081 ccctagtggt tatggttggt attatttttg aactatttgg ttcatatggt attattaaca
     1141 gtttactttc atcacttgga atggagccaa taagtttctt tacaaaatct gaatggttct
     1201 tgcctctcta cattggttca ggagtatgga aaacaatagg ttggggttct atcatttata
     1261 tgggagcatt aaccaatata gattcaactt tatatgaagc agcagatatg gacggggcta
     1321 atagatggca caaaatgtgg catgtaactc taccaagttt aagacctaca gtagttactt
     1381 tgtttatact aaatgctggt ggtatcatgc aagtaggatt tgaaaaggta ttcttgatgt
     1441 ctagtcctgc aacatatgaa gtatctgatg tattatctac ttatgtttat agacaaggta
     1501 ttttaaattc tgatttcagt tactcagctg cagtaggatt atttaataac atagtagctt
     1561 tgttatttgt tttattagct aataagattg ctaaaaaact tggcgaagaa ggaattgtat
     1621 aaggggggta aaa
//
LOCUS       DAVKZB010000182         1598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKZB010000182 DAVKZB010000000
VERSION     DAVKZB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.182"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>1598
                     /locus_tag="VXA80_001975"
     CDS             <1..>1598
                     /locus_tag="VXA80_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEV0354409.1"
                     /translation="LTTDLLQLKSCIEKYSKSELGEGILQYAEICEKMSHIFNEEKQR
                     YLKVDLVNSYDSLLPKDLKKTLEDFVNFISRINLGKDYRNKELISYTEKFVEKYGEYV
                     EVPIKQLLDSKLGLGIPKQNLEPYSILSSVAEQTFLSYLSKEIFKAVKNNKKEIDISN
                     IPPELLYPNLDRFAVNQFELYCEMKNFGEQPVISIVPNTGSDMIGKSIGRFASYFLNS
                     NIELDSRVDNVELIEFPSDNKNLNVMSSHHGHSKKLLLSYEDDFDIDSLELDFLVVGV
                     ERVNEHYKLYFRDLRTDLIVNFVTTSMLNHKSIGVFSHLARFLLTVSLEWQDNPFSLF
                     RVIENLDFLPYIPRIKYKNIILSEEKWILSDVDKKDMSTISQWKKFFDVPSLLYFHKD
                     DERLLIDLKNSLDVQWILKQNVDKLHFTRFDKIDGKNCEFIFGFENPRNSVYPHSVSE
                     KTVRRIENDFYKDYVKTFSSDWIYFKLYGINSSTMPELRENLLIFTDELLAEKLVSDF
                     HFVNYNDGGDGSIRLRFKIMNEDD"
BASE COUNT          567 a          181 c          291 g          559 t
ORIGIN      
        1 ttaactactg atttgttaca acttaaatca tgcatcgaga agtattcaaa atcagagtta
       61 ggagaaggta ttcttcaata tgctgaaatt tgtgaaaaaa tgtctcatat ttttaatgaa
      121 gaaaaacagc gctatctgaa ggttgactta gtgaatagtt atgattcgct attacccaaa
      181 gatttaaaaa agacattgga ggacttcgtt aactttattt cgagaattaa tctgggtaaa
      241 gattatcgaa ataaggaatt aatctcgtac acagaaaaat ttgtcgaaaa atacggagag
      301 tatgttgaag ttcctattaa gcaactactg gatagtaagt tagggttggg gataccaaaa
      361 caaaatttgg aaccttatag tatattatca agtgttgcag aacaaacttt tttgtcttat
      421 ctaagtaaag aaatctttaa agcagtgaaa aataataaaa aggaaattga tatatcaaat
      481 attccgcctg aattattgta tccaaatttg gatagatttg ctgtgaatca gttcgagttg
      541 tattgtgaaa tgaagaattt tggagaacaa cctgttatat caattgtgcc aaatactggg
      601 agtgatatga taggaaagtc tattggtaga tttgcttcat attttctaaa ttctaatata
      661 gaattagatt cgagggttga taatgtagaa ttaatagaat ttccaagtga taataaaaat
      721 ttaaatgtta tgtcttctca ccatggacat tctaaaaagt tattactatc ttatgaagat
      781 gactttgata ttgactcgtt agaattagat ttcttagtag tgggtgttga gagagtgaat
      841 gagcattaca aactatattt tagagactta cgaacagact tgattgttaa ttttgtaaca
      901 acctcaatgc tgaatcataa atctatcgga gtttttagcc atttagcgag atttttgtta
      961 acagtttcgt tagagtggca agataatcca ttttctctat ttagggtaat tgagaatctt
     1021 gatttccttc cttatattcc tagaattaaa tataagaata ttattttaag tgaagagaag
     1081 tggattttat cagatgtcga taaaaaagat atgtctacta ttagtcagtg gaaaaaattt
     1141 tttgatgtac ctagccttct atactttcat aaagatgatg agcgtttact gattgaccta
     1201 aagaatagtt tagatgttca atggatttta aaacaaaatg ttgataaatt acattttacg
     1261 cgttttgata agattgatgg taaaaattgc gagtttatct ttggatttga aaatcctcga
     1321 aatagtgtat accctcattc agtaagtgaa aaaactgtca gaagaattga aaatgacttt
     1381 tataaagatt atgtaaaaac gttttcgagc gattggatat atttcaaatt atatggaatt
     1441 aactcctcaa cgatgcctga actaagagaa aatcttctta tattcactga tgagctttta
     1501 gctgagaaat tagttagtga ttttcatttt gtaaattata atgatggcgg ggatggttct
     1561 attagattaa ggtttaaaat tatgaacgaa gatgattt
//
LOCUS       DAVKZB010000183         1563 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKZB010000183 DAVKZB010000000
VERSION     DAVKZB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.183"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            64..1410
                     /gene="gdhA"
                     /locus_tag="VXA80_001976"
     CDS             64..1410
                     /gene="gdhA"
                     /locus_tag="VXA80_001976"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV0354410.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          474 a          299 c          333 g          457 t
ORIGIN      
        1 agtgaaaaaa aattctgcta taatgtctta tgtaataaaa tatgacaata aaaggagaac
       61 gatatgacat ctgctaaaga atatatccaa agcgtgtttg aaactgtgaa agctcgtaac
      121 gggcacgagg ctgaattcct ccaagctgtt gaagaatttt tcaacacttt ggaacctgta
      181 tttgaaaaac accctgagta tatcgaagaa aatatcttgg cacgtattac tgagcctgag
      241 cgcgtggttt ctttccgtgt tccttgggtt gaccgtgatg gaaaaattca agtaaaccgt
      301 ggttaccgtg ttcaattcaa ctcagctgtt ggaccataca aaggtggact tcgtttccac
      361 ccaactgtaa accaagggat cttgaaattc ctcggatttg aacaaatctt taaaaatgtc
      421 ttgactggac ttcctatcgg tggaggtaaa ggtggatcag acttcgatcc taaaggtaaa
      481 acagatgctg aagtgatgcg cttctgccaa agcttcatga ctgaattgca aaaacacatc
      541 ggaccatcac ttgacgtacc tgctggtgat attggtgttg gtggacgtga aattggttac
      601 ctttacggtc aatacaaacg tcttaaccaa tttgatgctg gtgtcttgac tggtaaacct
      661 cttggatttg gtggtagctt gattcgtcca gaagcaactg gttacggttt ggtttactat
      721 actgaagaaa tgctcaaagc taacggtaac agctttgctg gtaagaaagt cgttatttca
      781 ggttctggta acgttgctca atatgctctt caaaaagcaa ctgaactcgg tgcaactgtt
      841 atttctgttt ctgactcaaa tggttatgtc atcgatgaaa atggtatcga cttcgatctt
      901 ttggttgatg ttaaagaaaa acgtcgtgct cgtttgactg agtatgcagc tgagaaagca
      961 actgctactt atcatgaagg ttctgtatgg acttacgctg gaaactatga cattgcgctt
     1021 ccatgtgcta ctcaaaacga aatcaacggt gaagcagcta aacgtttggt tgctcaaggc
     1081 gttatctgtg tatctgaagg tgccaacatg ccaagcaacc ttgatgccat caaagtttac
     1141 aaagaaaatg gtatcttcta cggacctgcc aaagctgcca acgctggtgg tgtagccgtt
     1201 tcagcccttg aaatgagcca aaacagcctt cgcctctcat ggactcgtga agaagttgat
     1261 ggccgtctca aagacatcat gacaaacatc ttcaacacag ctaaaacgac ttcagaaaca
     1321 tacggtcttg ataaagacta ccttgcagga gctaacattg ctgcctttga aaatgtagca
     1381 aacgctatga ttgcccaagg tattgtttaa gatttatttg cttaatcctc aatcactacg
     1441 attggggatt tttagttgag ttagacagtt gggaaattct aattaaatga aataaaaact
     1501 gaacaaattg attgcggaat tcaaaccaat ttctaacata cttctagaaa ttaatttgac
     1561 ttt
//
LOCUS       DAVKZB010000184         1551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKZB010000184 DAVKZB010000000
VERSION     DAVKZB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.184"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            25..424
                     /locus_tag="VXA80_001977"
                     /pseudo
     CDS             25..424
                     /locus_tag="VXA80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1189..1449)
                     /locus_tag="VXA80_001978"
     CDS             complement(1189..1449)
                     /locus_tag="VXA80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354411.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          532 a          288 c          267 g          464 t
ORIGIN      
        1 aacaaaatat ggtatagtag ttctatgaat tatgaagcaa gtaaacaatt aactgataca
       61 cgatttaagc gtcttgttgg tgttcagcgt accacttttg aagagatgtt agctggatta
      121 aaaacagctt atcaaaaaag tcggacaagc tggtaaaatc ttggctgaca gtaattatca
      181 agggctcatg aagatatatc ctcaagcaca aactccacgt aaatccagca aactcaagcc
      241 actaacagtt gaagataaag tctataacca tgcgctatct aaggagagaa gcaaggttga
      301 gaacatcttt gccaaagtaa aaacgtttaa aatgttttca acaacctgtc gaaatcatcg
      361 taaacgcttc ggattacgaa tgaatttgag tgctggtatt atcaatcatg aactaggatt
      421 ctagttttgc aggaagtcta ttatatatat gcacagacat atgaaatctt tttgatacag
      481 ccttcttttt acatagagag acagcagtta tgatatattt atccattcac aacatcttct
      541 gtaccaatac tacaataatt ggtacatttt gattacacaa tcgtttcaaa aaatatgatg
      601 gaagactgag tattttatag taattccctt cctatcaact agatcaacca gttgagtgtt
      661 gaatgttgaa acaccctagc ctcagcaaag gactatgatc agcatttcct agccaatagg
      721 acgctccaaa tcacctattc taagtccatc actagcttgg tcagtaagat acagactgca
      781 aggcgagact gatgtggttg atgagatttt ggaagagtat tacaatcata gatccaccag
      841 tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca cctctgcttc
      901 taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt ctcgtttcat
      961 tttactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa aaacgaagac
     1021 tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttaaaa aaccttgata
     1081 ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa gttttttcta
     1141 gtttccagat atagacgaat tcaatatagc catctcgcta caattatttt aaccatcgag
     1201 aaaatttttc agaatttcaa aaatttcatt ttgatccaat gaaatgtcat cattttgaag
     1261 aatagttgac ttcacaggct ctaattctat cacaaagcgg tgcatgtctg cttttaccgc
     1321 actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg tcaaacgggt
     1381 aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg tggcagagcg
     1441 ctccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat gaaaatcaaa
     1501 gagcaaacta ggaagctagc tgcaggctgc tcaaaacact gttttgaggt t
//
LOCUS       DAVKZB010000185         1538 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKZB010000185 DAVKZB010000000
VERSION     DAVKZB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.185"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(189..950)
                     /locus_tag="VXA80_001979"
     CDS             complement(189..950)
                     /locus_tag="VXA80_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV0354412.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SVADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(950..1426)
                     /locus_tag="VXA80_001980"
     CDS             complement(950..1426)
                     /locus_tag="VXA80_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354413.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
BASE COUNT          460 a          303 c          207 g          568 t
ORIGIN      
        1 atttttctat atttcgttgc agtccaatta gaaaatagct tgtaatcata agaaacaggt
       61 gtatgcaaat gaattaaagt cttgtgaaag ctaccgaatt tctctttgga atttgttagt
      121 tcttgataaa cagactttat cttgttatta aaccctctca tttctattct cctaaaaata
      181 tcaattgttt atttttcaag taattcatta agtctctttt cccccacctg caacatctcc
      241 ttctctacct gactccactc cccaaagagt aactcttgaa gaacatctgc tactgaagtt
      301 gtattttctt ttgaatcata tacacaactt ctatctcgtt ggtaaatttg aattctttca
      361 aagatagcta gttcttccaa ttttcgtgtg ttatcaacta gatgatttac aatgaaatca
      421 tgatgttctt ttggagttgc gcgtgcttga tttggattga taatgtacag ttcttcataa
      481 cggataagag tacttagata cgacaattca ggctttgtcg caattaaggc caattgtact
      541 tcatatccct tatttttcaa gagttgtgct gttttctttg gaacatcaac tgttcgtaaa
      601 gttccctcta tcaaaagatt gtatctcaaa ctactcaatt ttgttactaa agactctacc
      661 atttttcctg caaaatcttt ggtatattct acactgtctt tgccatattc ttgctgcagt
      721 tctaaatagt gtggatgctg agaacgaaaa ctatcaccat ctatgataac aatatttcct
      781 tgaaattctt tctgtttaat acgatgaatt gtagtcttac cggcaccact ttgccctcca
      841 agcaaaatcg ctataggttg cttactggac ttttttcctc ttgtcagtga acgaagattc
      901 cttgctaaag catgtttgaa ttcactatca gtataatctt ggatttccat taagctacca
      961 tccgcttttc agatatctct aacatacgtt caattccatc caaatagcca ctatatctct
     1021 ctatttcatc aaaagtttct actaaataga tattcgtatg aatcaagtca gatagatcat
     1081 cactcattaa aatccaagga ttagattcat catcaatgct aattccctga ctatcttgat
     1141 aacgatagag tcgagataat agattagctc ctctttcttt cacaatttca attttcaaag
     1201 tcaattcata atcttcaaca ggattgagca ttttatcttc tcctacaata tcgacataag
     1261 atacattaaa cttctgacaa atgatgtcaa ttaattccgt agagactgaa ctagttccat
     1321 tttcataacg actcaagcta tttcgagaaa ttcctataat tcgtgcaaat tcgggttgtg
     1381 ttaagtcatg tgttttacgt aaggatttta tgttctttcc aatcatagca aactccttta
     1441 tttttataaa tccattatag cataaaaaag cataacgcac caaaaatggt gcgttatttc
     1501 ttgtgtttct atcctaaact ttacttctat aaaagtca
//
LOCUS       DAVKZB010000186         1478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVKZB010000186 DAVKZB010000000
VERSION     DAVKZB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.186"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..776
                     /locus_tag="VXA80_001981"
     CDS             <1..776
                     /locus_tag="VXA80_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0354414.1"
                     /translation="DSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISS
                     TGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKA
                     VTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQ
                     HLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTI
                     APNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          472 a          210 c          304 g          492 t
ORIGIN      
        1 tcgactcttt tcctttaatt tggtttcata cgctgttaga taaagtgtta cggacgtgta
       61 aagaatttgg tgttaatgct atagttgagt actttggaga ggaggataca attagtaatt
      121 caattatatc aagcacaggt agcctagtag atggtgttat tgttttttat gaaagtgtag
      181 atgatatcag aattcagtat ttaaagaaga accatatgcc ttttcttgtt ttcggtgaat
      241 ctcaaacgtc tggagtagtc tatgtatcta ataataattt tcaagctact tatgatatga
      301 tgaaagctgt aactgaagaa aaatttaaaa atatgtggtt gcttatgggg ggagaatccc
      361 atgttaataa ggatcgtgaa agaggtgtgc gttcttttct gaatgataaa aattacttta
      421 tggatttaaa agtcatttat ggcttatcta caattgattc agtttattct tatgcaatgc
      481 agcacttgac tacccaaaat tatccggaca ttatttttgt ttcaggagat gaaaaggttc
      541 agggcttgat tcgtgcgtgc tatgagaaag gaattcttat tccagatgat atttctatta
      601 ttggatttga taatattcct atttctcagt attatactcc agctttgtct acaattgctc
      661 ccaattatgt taaattggct aaagaaatga tagaaggtgt tttagcaatt attaaagggg
      721 aaagtgtcac atctgttgaa gtttctccta aatttgttag gagacagagt ttctagtatt
      781 gtttaattct tgaaaatcct agtggaaaag atggagtgtt ctaaaataag ttctgtttag
      841 cctccattca gtttctattg agttggatcc aatttctaac aagtgggaca tgttcatgtt
      901 cgatctaagg aagacatggt cacattcgaa tgatattttc aaaaaaatcg taaaaaagtc
      961 ttgacaaaga aaaaacaaag tattaaaata agttcaacaa atcagaaaag taactatttt
     1021 tgatcttcag ggagcctgtg gtgattgtga ataggtggtt ggaagtagtg aaagtgggct
     1081 gattttgaaa atgaatttga aacaatgaaa attcggtgcg cacaccttac agtgcagctt
     1141 gttgttaaac aaggcagaga tgtaaaggga tagtcccttt ataattgagg tggcaccgcg
     1201 ttaccaacgc cctcacacgg aagtatattc tgtgtgtggg cttttttcta tccgtcgttt
     1261 ggtttatctt ttattagggc cttaagtcgc tttgatgaac ttgagttcta tctacagctc
     1321 cttgcctact actaaaagca aacaaaaagc tggactaata cgaaaaaagc tagtgatact
     1381 caatgaaaat caaagaacaa actaggaaac tagccgcagg ttgctcaaaa cactgttttg
     1441 aggttgtgga taaaactgac gaagtcagct caaaatac
//
LOCUS       DAVKZB010000187         1470 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVKZB010000187 DAVKZB010000000
VERSION     DAVKZB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.187"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(134..829)
                     /locus_tag="VXA80_001982"
     CDS             complement(134..829)
                     /locus_tag="VXA80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV0354415.1"
                     /translation="MKKKWFFADYYDTTIILLALISAILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIILVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLLGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(876..1130)
                     /locus_tag="VXA80_001983"
     CDS             complement(876..1130)
                     /locus_tag="VXA80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV0354416.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            complement(1132..1374)
                     /locus_tag="VXA80_001984"
     CDS             complement(1132..1374)
                     /locus_tag="VXA80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV0354417.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
BASE COUNT          484 a          289 c          188 g          509 t
ORIGIN      
        1 tgtcaagact tacttgattt ctcctcgaat caagttttat tttgtctcac ctttatgata
       61 ttttttcaac taagtctttt atcattcaat gtagttcttc gattttttca gattgtttct
      121 ctattaatat tctttagctc gataattcgt ctttgagttt atcaagtttt atctgttcat
      181 ctggattatc ctttacaaaa aagttcgtca aagcacttgt tagcatccct attgttccaa
      241 taccgacaag catcagtaaa actgctagcc atttccccaa aagggaaact ggaacaatat
      301 caccataacc aacagtagtt actgtcacaa gagcccacca aagactatct gagaaggatt
      361 tttcttcaac tacagataaa attgaacttc ctactagaat aataaagata ttaacatata
      421 agatataaat caggccattt gtccgtaaga atctactaat ttttctttct aatttacctg
      481 taagtccaat tattctaagt aaacgagtca gtttcagtaa ttttgttagt ttaactaaac
      541 gaaagatacg ccctaatcga aaaactgtga aaatagcatt taaagggaga atagccaata
      601 aatcaaaaat attttcaaga ataaagcgcc attttctttt agtaataaaa aagcgccaac
      661 tataatcaat tacaaaaaca ccccagatta ctaaatcaat aatactataa ggcggattgt
      721 ccaaatcaat catctcagca aatccaagta ataccagaat agcagagatt aaagcaagta
      781 aaataattgt tgtatcataa taatctgcaa aaaaccattt tttcttcaaa tttttactct
      841 ccttactttc tatattgtta ttactcagct ttaattcaat aaatatctct ccgatgacca
      901 acttctagag tagcaacaac taattcatca tctacaattt gtacgataac tcgataatta
      961 ccaattctat agcgccattg accaacgcga ttaccaacca aagcctttcc gtgtcgtctt
     1021 gggtcttcca aaacattgat ttgtaaatag tttgtaatta gcttctgcgt ataacggtcc
     1081 aattttttca attgcttgat gaaacgtctt gttggaacta atttatacaa attattcatc
     1141 cttcaagcct aaatcatgca tcatttcttc ccaagtaatg ggttcaactc ctttttccaa
     1201 gtcttctaaa tactcttgat aggctaaatc tgccacacga gcatcgtatt catcttctag
     1261 ggcttcaaga gttttggtgc gaataagttc cgaaagggaa actccttcaa acttagccat
     1321 tgctttcata aatgttttat cagcttcaga aacttttaat gtaatagtag tcatcttttg
     1381 tgctcccttt tttaatggta acaccattgt attacttttt aggtgttcag tcaatataaa
     1441 aagaacacct tctcagcgtt ctttctatat
//
LOCUS       DAVKZB010000188         1425 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVKZB010000188 DAVKZB010000000
VERSION     DAVKZB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.188"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..191)
                     /locus_tag="VXA80_001985"
     CDS             complement(<1..191)
                     /locus_tag="VXA80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0354418.1"
                     /translation="MGKSVAILMTTYNGERYLSQQIDSIRSQTFTNWTLFIRDDGSKD
                     KTIEVIQRYSKIDDRIRLV"
     gene            complement(194..913)
                     /locus_tag="VXA80_001986"
     CDS             complement(194..913)
                     /locus_tag="VXA80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV0354419.1"
                     /translation="MSIYKLCKDIERKTMSPAKKAMAKNDYFAFYVGRPLSYLLTVPF
                     VKTNITPNQISYLSIIPLIVGFIIMIFTTDFVVLLLAWFLFFLWNLLDGVDGNLARYR
                     EQYSKDGSVVDAMAGYVAMVLTYFGAGIVAAHLTDSDIYIILGALSGFSLIFPRLVMH
                     KYINTVAQDESVSSIKDKSDFNTIKILALNMTSITGIPQVLLLLTILTNQWVLFTLVY
                     FTINFLLMIFSLYSLFKKENV"
     gene            complement(900..>1425)
                     /locus_tag="VXA80_001987"
     CDS             complement(900..>1425)
                     /locus_tag="VXA80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0354420.1"
                     /translation="SEQKNPFFFVEFAKKLSEIYSNLYFVIVGDGELRGRTEELIEEY
                     GLRSSFFITGWVDNPEDYLAQFNQAVLFSKWEGFGLAVAEYMKHKKPILITNVDGMSE
                     LVIDGESGFKVPLYNLEVTVDRSRSIIENRELANELGSAAFQRVRSTFEIKEKVSELE
                     NIFMSLREDDNVNI"
BASE COUNT          487 a          289 c          178 g          471 t
ORIGIN      
        1 tcaactaatc taattctatc atctatctta gaatacctct gtattacttc tattgtttta
       61 tcttttgatc catcatccct aataaaaagc gtccaattag tgaatgtttg agacctaata
      121 ctatcaatct gttgtgacaa atatcgctca ccattatagg tggtcattaa aattgcaact
      181 gactttccca tttctaaaca ttctcctttt tgaataatga atacaaagaa aatatcatta
      241 ataaaaaatt aatcgtgaaa tatactaaag taaaaagtac ccactgattt gttaaaatag
      301 ttaatagcag taaaacctgc ggaattcctg taattgatgt catgtttaga gccagtattt
      361 ttatagtatt aaaatcggat ttatctttaa tgctactcac agactcatct tgagctactg
      421 tattgatata cttatgcatc actaaccttg gaaaaatcaa tgaaaaccca gataatgcac
      481 ccaggattat atagatatct gagtcggtta aatgagcagc tactattcct gcaccgaaat
      541 acgtcaacac catggctaca tagccagcca ttgcatctac tacacttcca tccttcgagt
      601 attgctcccg atatctggct aagttcccat ctactccatc tagtaagttc cataaaaaaa
      661 atagaaacca tgccagtaat aatacaacga aatcagttgt aaatatcatt attataaatc
      721 caacaatcaa aggaattata gataaataag atatttgatt gggagtaata tttgttttca
      781 cgaaaggaac tgttaaaaga taggataaag gtcttccaac ataaaatgca aaatagtcgt
      841 ttttagccat tgctttttta gcaggcgaca tagtttttct ttcaatatct ttacaaagtt
      901 tatatattga cattatcatc ctctcttaaa ctcatgaata tattctctaa ctctgacact
      961 ttttctttta tttcaaatgt agatcgaact ctttggaaag cagcactacc taactcattg
     1021 gctagttctc tattctcaat aatacttcta cttctatcta cagttacttc taaattatat
     1081 agtgggactt taaaacctga ctcaccatca ataaccaatt ctgacatccc atcaacatta
     1141 gttataagaa ttggcttctt atgtttcata tattccgcaa ccgccaatcc aaagccctcc
     1201 catttcgaga aaagaactgc ctgattgaac tgagctaaat aatcctctgg attatccacc
     1261 caccctgtta taaaaaatga gcttcgaagc ccatactctt caattagttc ttcagttcgc
     1321 ccacgcaatt cgccatcgcc gacaataaca aaatataaat tgctataaat ctctgataat
     1381 ttttttgcaa attcaacaaa aaagaaagga tttttctgtt cacta
//
LOCUS       DAVKZB010000189         1405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVKZB010000189 DAVKZB010000000
VERSION     DAVKZB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.189"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..459)
                     /locus_tag="VXA80_001988"
     CDS             complement(<1..459)
                     /locus_tag="VXA80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354421.1"
                     /translation="MSEVDFNEAVTYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCIGDFPHDFF
                     EIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKN"
     gene            complement(517..1230)
                     /locus_tag="VXA80_001989"
     CDS             complement(517..1230)
                     /locus_tag="VXA80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV0354422.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          460 a          262 c          230 g          453 t
ORIGIN      
        1 atttttttct aaaaagtcgt caaaattaga aaaaaatcga aaatacaatg cattctcttg
       61 gtgtaaaatc gctcttttta aaggataata ctctttaata ttattaactt tttgttcgta
      121 ggcatatttt gctacatgat ctaggtatat ctcaaaaaag tcatgaggaa aatctcctat
      181 acagccatta cgattcatat tgaggctata ttttcgatta gtaagataat tcggaaatat
      241 atttcctatt actctaagat tatctacttg tgaatccctt ctcccaatat aatccccttc
      301 tttgactaat gatggagatt tccaagtaaa aattaaatcg ccagagaaat ttttcttatc
      361 aaaaaactta aaaagagatt gataaatact atttgctaat tgacaagtgt cagatgtaaa
      421 ttcataagta acagcttcat taaaatcaac ttcagacatt tcaatgtact cttcaatagt
      481 aatattagct tttttgtagt cattagctcg tctcctttaa aatgtgatct tttcaagaat
      541 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      601 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      661 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      721 tccagcaatt tgctggccaa tcttttcata aaaaagagca tccggctttt gagtttgcaa
      781 ctgttctgag ataaagactt gattgaaata aggtgctaga ccagattgag ccaaacgtcc
      841 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag ttataatcac gctcaatgag
      901 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgggcga ggtaaaattg
      961 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagcaa ataaacgaga
     1021 aaagcgcgtg ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag
     1081 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
     1141 ttttagaagt tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
     1201 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
     1261 agaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaaatt
     1321 cgaacacttt ctatacaact gacagctcaa atctttcaga atagaacaat actaactatc
     1381 gagcacccca tcttcataaa tatat
//
LOCUS       DAVKZB010000190         1398 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVKZB010000190 DAVKZB010000000
VERSION     DAVKZB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.190"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(212..598)
                     /locus_tag="VXA80_001990"
     CDS             complement(212..598)
                     /locus_tag="VXA80_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV0354423.1"
                     /translation="MESIGIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEG
                     GGIGTSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAY
                     NAAALLQAGAELSVIQIQLAELEINK"
     gene            complement(1020..>1398)
                     /locus_tag="VXA80_001991"
     CDS             complement(1020..>1398)
                     /locus_tag="VXA80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV0354424.1"
                     /translation="RNKNGQADTNQTEKPQTEKPEEETPREEKPQSEKPESPKPTEEP
                     EEESPEESPEESEEPQVETEKVKEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          404 a          279 c          211 g          504 t
ORIGIN      
        1 ttttaatttt tgaaggattc taaagctaga atagccccat cacaatcagt tttgattgat
       61 tcacaattta gaaacactat agtttcactc ctgttaaaat aaaaaggaat tgcataaagc
      121 aatccctttc tgattttgaa atcatttact taacatttta tagttgagat aatcaatagc
      181 ttacctataa aaagagttat agtaaaattc cttatttatt gatttcaagc tccgctaact
      241 gtatttgaat aactgacagt tctgcaccag cctgaagaag agcagctgca ttataggcac
      301 cttctacaat tggaaccctg ttgatgatga tacttttatc actgaaatca gtcaccattt
      361 ttaagttcat tttagcagaa cctaggtcaa aaaatacaag taaagtatct gctggatttt
      421 cggaaacaac cctatctact tgatcaaaac tcgttccaat tcctccgccc tcggttcctc
      481 ctacataagt aatcggaaca tctttagcta ctttactaat cagttcaaca acaccttctg
      541 caatgtgttt ggaatgtgaa acgataacaa gaccaatacc aataccaata ctttccatca
      601 aaccacccca gtttctaaaa tagcagtaaa gaataatcct gatgagaatg atccaggatc
      661 aatatgtcca agaaaccaca tgctcctaag acaagagcta acagactggc catcaataat
      721 agtattgttc tttttttcat cattactcct taactagtgt ttaactgatt aattagccag
      781 taaatagttt atctttattt acactatctg ttaagataca gtaaaatgaa ataagaacag
      841 gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtatactatt ctagtttcaa
      901 tctactatat tttgcacatt ttcatgaaaa aaatgagaac tagaactcac attctgctct
      961 catttttcgt tttcccgttc tcctatcctg tttttaggag ttagaaaatg ctgctacctt
     1021 tacttactct cctttaataa agccaatagt ttttcagctt ctgccataat agtattgttg
     1081 tcctgggtgc caaatagtaa attatttttt aatcctgtga gagtctcttt ggcattggac
     1141 ttgataattg gatcctggat ttttccaagt aaatcttcag cctctctcag tttttcttta
     1201 accttttcag tctcgacctg aggttcttct gattcctctg gtgattcttc tggtgattct
     1261 tcttctggtt cctctgttgg ttttggagac tccggtttct cgctttgcgg tttctcttct
     1321 cgaggggttt cttcctcagg tttttctgtc tgaggttttt ccgtttgatt ggtatcagct
     1381 tgaccatttt tgtttctt
//
LOCUS       DAVKZB010000191         1372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVKZB010000191 DAVKZB010000000
VERSION     DAVKZB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.191"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            56..1117
                     /gene="dinB"
                     /locus_tag="VXA80_001992"
     CDS             56..1117
                     /gene="dinB"
                     /locus_tag="VXA80_001992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV0354425.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
BASE COUNT          446 a          258 c          292 g          376 t
ORIGIN      
        1 gatagagctg acgtggtttg aagagatttt cgaagagtat aaaaaggtgc taggcatgtt
       61 gatttttcct ttgttaaatg atttgtcaag aaaaatcatc catattgaca tggatgcctt
      121 ttttgctgca gtggaaatca gggataatcc taaactcaga ggaaaacctg tcattattgg
      181 aagcgaccct cggcaaacag gtggacgggg agtcgtttct acctgtagtt atgaggcaag
      241 agcttttggt gtccattctg ccatgagttc caaggaagct tatgaacgtt gtccccaggc
      301 tgtctttatc tcagggaatt atgagaaata caagtctgtg ggactccaga ttcgagctat
      361 ttttaagcgc tatacagatt tgattgaacc catgagcatt gacgaagcct atttggatgt
      421 gacagaaaat aaactcggta tcaagtcagc ggtcaaaatt gctcgcctca ttcaaaaaga
      481 tatctggcaa gaactccatc taactgcttc cgcaggcgtt tcttacaaca aattcttagc
      541 taaaatggcg agtgattatc aaaaaccaca tggtttgaca gtgattctac ctgaacaggc
      601 tgaggatttt ctcaaacaaa tggatatttc caaatttcat ggagtaggaa aaaagacagt
      661 agaacgtctt catcaaatgg gcgtttttac tggtgctgat ttacttgaag ttcctgaggt
      721 aaccctaata gaccgttttg gtagactagg ctatgatctg tatcgaaagg ctcgtggcat
      781 tcacaactct ccagtcaaat ccaatcgcat ccgtaaatca attggcaagg agaaaaccta
      841 cgggaagatt ctccttgctg aggaagatat caaaaaagag ctgactcttc tatcagaaaa
      901 agtcgctctc aatctacatc aacaagaaaa agctggaaaa attgtcattt tgaaaatccg
      961 ctacgaggac ttttcaactc ttaccaaacg aaaaagtctt gctcaaaaaa cacaagatgc
     1021 tagtcagata agccaaatag ccctgcaact ctatgaagaa ttaagcgaga aagaaagagg
     1081 tgtccgccta ttggggatta ccctgactgg attttaaagc tcaatgaaaa tcaaagagca
     1141 aactaggaag ctagccgcag gctgctcaaa acactgtttt gaggttgcag ataaagctga
     1201 cgcggtttga agagattttc taagagtata aaaccttgaa gagttgcccc ttcaaggttt
     1261 tttcttaggc tctataatat ttgtagtggg taaatccact atagatatta tggagcctat
     1321 tttattgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa cc
//
LOCUS       DAVKZB010000192         1370 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVKZB010000192 DAVKZB010000000
VERSION     DAVKZB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.192"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            19..168
                     /gene="blpO"
                     /locus_tag="VXA80_001993"
     CDS             19..168
                     /gene="blpO"
                     /locus_tag="VXA80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV0354426.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCTAGVAYGAIDG
                     CATTV"
     gene            272..391
                     /locus_tag="VXA80_001994"
     CDS             272..391
                     /locus_tag="VXA80_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0354427.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            688..852
                     /locus_tag="VXA80_001995"
     CDS             688..852
                     /locus_tag="VXA80_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354428.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            849..1253
                     /locus_tag="VXA80_001996"
     CDS             849..1253
                     /locus_tag="VXA80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV0354429.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          422 a          183 c          276 g          489 t
ORIGIN      
        1 tgaaaagtga tatactatat ggatacaaaa atgatgtcac aatttgcagt tatggataat
       61 gaaatgcttg cttgcgttga aggtggagat attgattggg gaagaaaaat tagttgtaca
      121 gcaggggttg catatggcgc aattgatggg tgtgcaacaa cggtttgaca tttctattgg
      181 gaccatttgc tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggtg
      241 tagcctatgc agcaatatgt tggtggtaat tatggatttt aaaagtttta ttattggttt
      301 agtagttggt atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc
      361 ttccaaaaag gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag
      421 cttaatttac aggggagttt ctttaatttt gtttcgcctg tcaaaatgat atgtgatagc
      481 aggatgaaaa gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt
      541 tcatgcttgc ttttttaatt tttacaattc aagaggtttt gatgaccatt tatgatttga
      601 gtgatccaag gtcaaaataa gtgctatact agcagtgtaa atgttcttgc tcaactaaga
      661 taaatcgcac atttaggagg aaatcttatg aagaaaaaaa tactgattat tttcgttctg
      721 tatctaatca tgtccatctt tctatatccg cttagggaga gtgcttggta tcagctattt
      781 tataccatag cctatgtgat tgcagttatg atctattttg ctatcaataa aaagaaagga
      841 gaaaagaaat gaaaactttt cttgctaaaa aacggaacat cttccttgcg agattgttct
      901 taggtcagtt gcccttgctt gtttctactt atctatttct atctcgtcag tttttaaatt
      961 tttccgtagt tttccaattt cttttagtag ttattaactt ggcttctatt ttggttactg
     1021 tttatctcac tagggaaatg aggataagag agtttgaaga tgatgatttg gttagtccta
     1081 gaaccaatca acttatgttc atcggcttga caggttttat gtctattatt tgtttgtata
     1141 gaggtatcac agcaggagaa tcctaccaac aaataatagc ttatattggc gctgttctct
     1201 gcttaatcat catgcttctg ctcatgtggg gcttgaagta ttataaaaag tagagcttaa
     1261 tgaagtataa aagaaaaggg taagtgtttt tgcaatactt acccttttat aatagattcc
     1321 ctgcgaaaca aaatatggta tagtatagta gattgaaact agaatagtac
//
LOCUS       DAVKZB010000193         1349 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVKZB010000193 DAVKZB010000000
VERSION     DAVKZB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.193"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>1349
                     /gene="pcpA"
                     /locus_tag="VXA80_001997"
     CDS             <1..>1349
                     /gene="pcpA"
                     /locus_tag="VXA80_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV0354430.1"
                     /translation="VILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTVE
                     GNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETGR
                     IVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELPA
                     SLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNLF
                     RLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELASYS
                     FNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELIL
                     PDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVKK
                     DTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGV"
BASE COUNT          524 a          188 c          220 g          417 t
ORIGIN      
        1 ggttatttta gcagcatatg tccctaatga accaatccta gcagatactc ctagttcgga
       61 agtaatcaaa gagactaaag ttggaagtat tattcaacaa aataatatca aatataaggt
      121 tctaactgta gaaggtaaca taggaactgt tcaagtgggt aatggagtta ctcctgtaga
      181 gtttgaagct ggtcaagatg gaaaaccatt cacgattcct acaaaaatca cagtaggtga
      241 taaagtattt accgttactg aagtagctag tcaagctttt agttattatc cagatgaaac
      301 aggtagaatt gtctactatc ctagctctat tactatccca tcaagcataa aaaaaataca
      361 aaaaaaaggc ttccatggaa gtaaagctaa aactattatt tttgacaaag gcagtcagct
      421 ggagaaaatt gaagatagag cttttgattt ttctgaatta gaagagattg aattgcctgc
      481 atctctagaa tatattggaa caagtgcatt ttcttttagt caaaaattga aaaagctaac
      541 cttttcctca agttcaaaat tagaattaat atcacatgag gcttttgcta atttatcaaa
      601 tttagagaaa ctaacattac caaaatcggt taaaacatta ggaagtaatc tatttagact
      661 cactactagc ttaaaacatg ttgatgttga agaaggaaat gaatcgtttg cctcagttga
      721 tggtgttttg ttttcaaaag ataaaactca attaatttat tatccaagtc aaaaaaatga
      781 cgaaagttat aaaacgccta aggagacaaa agaacttgca tcatattcgt ttaataaaaa
      841 ttcttacttg aaaaaactcg aattgaatga aggtttagaa aaaatcggta cttttgcatt
      901 tgcggatgcg attaaacttg aagaaattag cttaccaaat agtttagaaa ctattgaacg
      961 tttagccttt tacggtaatt tagaattaaa agaacttata ttaccagata atgttaaaaa
     1021 ttttggtaaa cacgttatga acggtttacc aaaattaaaa agtttaacaa ttggtaataa
     1081 tatcaactca ttgccgtcct tcttcctaag tggcgtctta gattcattaa aggaaattca
     1141 tattaagaat aaaagtacag agttttctgt gaaaaaagat acatttgcaa ttcctgaaac
     1201 tgttaagttc tatgtaacat cagaacatat aaaagatgtt cttaaatcaa atttatctac
     1261 tagtaatgat atcattgttg aaaaagtaga taatataaaa caagaaactg atgtagctaa
     1321 acctaaaaag aattctaatc agggagtag
//
LOCUS       DAVKZB010000194         1349 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVKZB010000194 DAVKZB010000000
VERSION     DAVKZB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.194"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            28..993
                     /locus_tag="VXA80_001998"
     CDS             28..993
                     /locus_tag="VXA80_001998"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV0354431.1"
                     /translation="MAIIKKIVVLYGGLSEEREVSENSAKEISKSLLTLGYDVISIDL
                     SQDCSYEIGEIEKSERGLNKQKAEIGSGIIDVCRKVDIVFLATHGGIGENGKLQAIFD
                     VEKIDYTGNSFLSTAISMDKKLSKIVASSVGIKCASNLMVDRIQANDFPIVIKPIRSG
                     SSKGIKILQNKKQFDIYYKEHPELGSFFVEKYIKGREFSVGILGETVLPVIEIKVKNG
                     FYDYNNKYTVGAAEEVVPAKISEKLTHTLQKSAYKIHKALGFNVYSRTDFIVDEKGDV
                     YFIESNSLPGMTKTSLLPQEAKAAGIDFPNLCERIIELSREIRSQ"
     gene            1058..1294
                     /locus_tag="VXA80_001999"
     CDS             1058..1294
                     /locus_tag="VXA80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354432.1"
                     /translation="MKIINGICRYIFDSKGFATIEVEIFLDSGDTGIGAAPRGSTTGR
                     YDIQYNEYYPRGNNFSPIPDGHIEFFRLYNICSG"
BASE COUNT          489 a          156 c          282 g          422 t
ORIGIN      
        1 taagattcta ataaggaggt taagtgaatg gctattatta aaaaaattgt tgtactatat
       61 ggtggcctta gcgaagagag agaggtgtca gaaaattctg caaaggagat tagtaaaagt
      121 ctattaacat tggggtacga tgtgatttct atagatttat ctcaagactg ttcgtacgag
      181 ataggagaaa ttgaaaaaag tgagcgcgga ttaaataaac aaaaagctga gattggttcg
      241 ggtattattg atgtttgtag aaaggttgat atagttttct tagcaactca tggcggaata
      301 ggtgagaatg ggaaactaca ggctattttt gatgtagaaa aaatagacta tacaggaaat
      361 tcgtttctta gtacagctat tagcatggat aaaaaattat caaaaatagt ggctagttct
      421 gttggaataa aatgtgcttc gaacttgatg gttgatagaa ttcaggcaaa tgattttcct
      481 attgttataa agccaattag aagtggttca agtaaaggta ttaagatact tcaaaataaa
      541 aaacaatttg acatttatta taaagagcat cctgaacttg gttcattctt tgtagaaaag
      601 tatattaagg ggagagaatt ttcggtaggt attttaggtg aaactgtttt accggttatt
      661 gaaataaaag taaaaaatgg tttttatgat tataataaca agtacactgt tggtgcagct
      721 gaagaagtcg ttcccgcaaa aatatctgaa aagttaactc ataccttaca aaaatccgca
      781 tataaaatac ataaggctct tggatttaat gtttattcac gtactgattt tattgtagat
      841 gagaaggggg atgtatattt tattgaatca aatagtttac caggtatgac taaaacaagt
      901 ttattaccac aggaagcgaa agcagctggt atagatttcc caaacctatg tgaacgtatt
      961 attgagctga gtagggaaat tagatctcaa tagaaaataa aatcgacgac atttataaaa
     1021 aagtaatttt caatgataaa aaagaaagtg gaagcctatg aaaataatta atggtatttg
     1081 cagatatatc tttgactcaa aagggttcgc aacaattgaa gtagagattt ttcttgatag
     1141 tggagatact ggaattggtg ctgctccacg agggtctaca actgggcgtt atgatattca
     1201 atataatgag tattatccta gaggaaataa ctttagtcca ataccagatg gacatataga
     1261 atttttcagg ctctataata tttgtagtgg gtaaatcccc tatggatatt atggagccta
     1321 ttttgtgtag aaaaaaagtc ccatatgac
//
LOCUS       DAVKZB010000195         1337 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVKZB010000195 DAVKZB010000000
VERSION     DAVKZB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.195"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            17..856
                     /locus_tag="VXA80_002000"
     CDS             17..856
                     /locus_tag="VXA80_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0354433.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMETILAHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            963..1238
                     /locus_tag="VXA80_002001"
     CDS             963..1238
                     /locus_tag="VXA80_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV0354434.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          442 a          207 c          289 g          399 t
ORIGIN      
        1 tagaaagatt gatactatga caaaaattaa gattgtaacc gattcatctg ttactattga
       61 acctgaacta gtaaagcaat tagatattac aattgttcca ttatctgtaa tgattgataa
      121 tgttgtttat tctgatgcgg atttgaaaga agaaggtaaa tttcttcagt tgatgcaaga
      181 aagtaagaat cttccgaaaa caagtcagcc acctgtaggt gtctttgctg agatttttga
      241 agacctatgc aaagatggtg gccagattct tgctattcat atgtcccatg ctctttcggg
      301 tacggtagaa gcagcacgcc aaggtgctag cctatctact gcagatgtga ctgttgttga
      361 tagttccttc actgaccaag ccctgaaatt ccaagttgtt gaggctgcga agttagctca
      421 agaaggtaaa gatatggaga caattttagc tcatgtagaa gaggttaaaa accatacaga
      481 gctttatatt ggtgtttcaa ctttggaaaa tcttgtcaaa ggtggacgaa ttagccgtgt
      541 aactggcttg ttgagctctc ttctcaatat ccgtgctgtc atgcaaatga aagaccatga
      601 attgcagcca atggttaaag gtcgtggaac taaaacattt aaaaaatggt tagatgagtt
      661 gataacatcg ctttctgaac gtgctgtagc agagattgga atttcatatt ctggtagtga
      721 tgattgggca aaagagatga aagaaagctt acaagcttat gttgaaaagc caatttctgt
      781 tttggaaacg ggttccatta ttcaaactca cacgggtgag aacgcttggg ctattttgat
      841 acgttaccac tcctaaaaaa ataaataaaa tgggaagaaa tagcgttttt aacttgacct
      901 aaaagggatt ttaggatatg attatatttg ttaattagaa attatttgga ggaatcatta
      961 acatggcaaa caaacaagat ttgatcgcta aagtagcaga agctacagaa ttgactaaga
     1021 aagactcagc agcagcagtt gaagctgtat ttgcagcagt agctgactat cttgcagctg
     1081 gtgaaaaagt tcagttgatc ggttttggta actttgaagt tcgtgagcgc gcagaacgta
     1141 aaggtcgcaa cccacaaact ggtaaagaaa tgacaattgc agcttctaaa gtaccagcat
     1201 tcaaagctgg taaagctctt aaagacgctg ttaaataatc agtctttaaa aagcctattg
     1261 tatcaagctt tctagcttgg tcagtaggct ttttaattat gaaatctagt aataaataga
     1321 gttaaaaagt tgaatgt
//
LOCUS       DAVKZB010000196         1322 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVKZB010000196 DAVKZB010000000
VERSION     DAVKZB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.196"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>1322
                     /locus_tag="VXA80_002002"
     CDS             <1..>1322
                     /locus_tag="VXA80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0354435.1"
                     /translation="SKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHV
                     KSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESIC
                     KEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFL
                     EWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDS
                     FNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCV
                     KKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQ
                     KGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLL
                     QNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKES"
BASE COUNT          442 a          184 c          214 g          482 t
ORIGIN      
        1 tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa cataaacgtt
       61 ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca gtcaaagatg
      121 atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct actaatggta
      181 tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat ttctttaaac
      241 attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt caagctgaga
      301 gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt agccaaatca
      361 ataaagtgat taaaaggcaa tttcaatttg aagttagtct gacccctgtt caaatcattg
      421 gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa tattatttcc
      481 tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta gaattggttt
      541 ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag ttgctcctag
      601 ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa gattctttta
      661 acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt gctcagagtt
      721 ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta tttgtgtctt
      781 actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta aaaaaggata
      841 gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt tcagtcaagt
      901 atcagattga aatggagaat aaggataatc tgatttggca tctgcataat accgcacatc
      961 tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg aatacaatca
     1021 ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag ctttctcact
     1081 atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta tcttatacct
     1141 tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct aaactaaagg
     1201 ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag acactttctt
     1261 attattgtag caataatttt gaactggaag tttggactga attagaatta tcaaaggaat
     1321 ct
//
LOCUS       DAVKZB010000197         1301 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVKZB010000197 DAVKZB010000000
VERSION     DAVKZB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.197"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..815
                     /locus_tag="VXA80_002003"
                     /pseudo
     CDS             1..815
                     /locus_tag="VXA80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(850..1059)
                     /locus_tag="VXA80_002004"
                     /pseudo
     CDS             complement(850..1059)
                     /locus_tag="VXA80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1225..1298)
                     /locus_tag="VXA80_002005"
     tRNA            complement(1225..1298)
                     /locus_tag="VXA80_002005"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1262..1264),aa:Asn,seq:gtt)
BASE COUNT          433 a          269 c          206 g          393 t
ORIGIN      
        1 agtataaaaa aattcaaact agaattcttc ttagaaaagg ccgttttaag tgctatcatt
       61 gctcaaaaat ggagctcgct gagacttcta tcgtcaagaa gaatcatcaa attccttgta
      121 tcatcaacca aaagattgct cagaagctaa ttaaaaagac ttctatgacc gacattgatc
      181 atcagttgtc tatttcaact tcaactgtca ttcgcaagat caatgatttt cactttgagc
      241 atgatttttc gcgtcttcct gagattatgt cctaggacgt tgaaacagtc cggggagtga
      301 ctgtttcaat cgggagatgg agatgagctt tattgctcaa gactttgata agctcaatat
      361 cataactgtt cttgagagca gaacacaagc catcatccga aatcccatga atacaaggct
      421 atcaagcgat actgaaagct cattcaacaa gatagtcaga aactgagtga taaacgattt
      481 tatcgcccta cttttcgtat gcacttgact aataaagaaa ttctagataa acttttgagc
      541 tattccgaag acttgaaaca ccactataat ctctatcagc tcttgctttt cctcctcttt
      601 ttcagactct gtttaaaacc ttcctcaaag ataaagagaa aatcgtcaac gcccttcaat
      661 taccttattc caacgccaaa ctggaagcca ccaataatct catcaaactt atcaagtaca
      721 atgcctttgg ttttaggaac tttgaaaact tcaaaaaaga aaggacgaaa tttgtccttt
      781 ctagatcttc gctttcttca acccactaca gttgacaaag aaccgaaaaa tgtctcgcct
      841 ctaatatagt caattgaaac aagaacaaga caaaagagcc tcataaaagg tattgcaact
      901 tggtaatacc tttttggggt tctttttgat atgaacccat gttttctcaa taggattgta
      961 ctcaggtgag tagggaggaa gaggtaaaag tttatgccca aactcttcac acaagagttc
     1021 tagcttaccc attctatgga atcttgcatt gtccataata tagtagattg aaactagaat
     1081 agtacacctc tacttctaaa acattgttag aattcgattt tactgtcctg atcgatttgt
     1141 cctattctta tttcatttta ctataacacg aaatagatta ttattacata acaaaaaaga
     1201 ggtctaaacc tcttaactaa attactccgc cagtaggact cgaacctacg acatcatgat
     1261 taacagtcat gcgctactac caactgagct atggcggatt a
//
LOCUS       DAVKZB010000198         1264 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVKZB010000198 DAVKZB010000000
VERSION     DAVKZB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.198"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..553
                     /locus_tag="VXA80_002006"
     CDS             <1..553
                     /locus_tag="VXA80_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354436.1"
                     /translation="YLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNM
                     VSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGD
                     DGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIV
                     THAPHPELKQYFTKIYQFPKDFI"
     gene            573..>1264
                     /locus_tag="VXA80_002007"
     CDS             573..>1264
                     /locus_tag="VXA80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354437.1"
                     /translation="MRFYCEAYEVITEEKKVYFNDIELEVKYSSVEELFIICEKLLDK
                     KKVSFFYVDEKPLRYLLFDYIFLLVLAKKNIPILDGVSNKQVDPTLLHHFSLEIEKNF
                     IDFCYKNMDLILKTQSISLCHREELIIVDVSDDSKFGVFYKRFRTLVDKNNGKYVCVY
                     NFSRVSDILQNWKNYCNRKFSVTFTESQFELFKLLYNQKNFKTISLLFGKKIVAGGII
                     YYSDLTNIEYFC"
BASE COUNT          450 a          134 c          235 g          445 t
ORIGIN      
        1 gtatttaatt ttaggtgaaa gtggaagcgg gaaatctaca ttattaaaat tattgaatgg
       61 ctttttgaga gattatagtg gagaattgcg attctgcggg gatgatataa aaaaaacctc
      121 ctatttaaat atggtttcga atgttctata tgtagatcaa aaagcttatt tgtttgaagg
      181 tacgattaga gataatattt tattggaaga aaattatact gatgaagaaa tactacagtc
      241 tttagagcaa gttggtttga gtgtaaaaga ttttcctaat aacattttag attattatgt
      301 tggtgatgat gggagattac tgtcaggagg gcagaaacaa aaaattactt tagctagagg
      361 gctaattaga aataagaaaa tagtattaat tgacgaggga acttctgcta tcgataggag
      421 aacttcgtta gcgattgaac gtaagatatt agatagagag gatttgactg tcattattgt
      481 tacccatgct ccgcatccgg aacttaaaca atattttact aagatatatc aatttccaaa
      541 ggattttatt taagaatata ggggagggaa acatgagatt ctattgtgaa gcttatgagg
      601 taattactga agagaaaaaa gtatacttta atgatattga gttagaagtt aagtattcct
      661 ctgtcgaaga attgtttata atatgtgaga aattattaga taagaaaaaa gtatcatttt
      721 tttatgtaga tgagaaaccc ttaaggtatc tgttatttga ctatatattt ctactagttc
      781 tagctaaaaa aaatatccca atcttagatg gagtttccaa taagcaagtt gatccaacgt
      841 tgctgcatca cttttctttg gaaattgaaa agaattttat cgatttttgc tataaaaata
      901 tggatctcat cctaaaaact caaagtatat ctttgtgtca tagagaagag cttattattg
      961 tagatgtttc agatgactct aaatttggtg ttttttacaa gcgttttaga acactggttg
     1021 ataaaaataa cggaaaatat gtttgtgttt ataatttcag tcgtgtttca gatatactgc
     1081 aaaactggaa aaattattgc aatcgaaaat tttcggttac gtttactgaa agccaatttg
     1141 aattatttaa gcttttatat aatcaaaaaa attttaaaac aatttcgttg ctatttggaa
     1201 aaaaaattgt tgctggagga attatctatt atagcgatct tactaatata gaatactttt
     1261 gtat
//
LOCUS       DAVKZB010000199         1190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVKZB010000199 DAVKZB010000000
VERSION     DAVKZB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.199"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(480..1106)
                     /locus_tag="VXA80_002008"
     CDS             complement(480..1106)
                     /locus_tag="VXA80_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyl transferase"
                     /protein_id="HEV0354438.1"
                     /translation="MVYSDMSTIDASNNLIDISINKIMGIELPNINNLYFIQAYIWGC
                     TAGFNHALLEMVPSVDIDKDYLYIEKLAHDSYFAKFALEYGKVLFCPEQLVLYRRHGH
                     NVTTSHHFKLSPLNVFRKAILGFNELALTHARVYNQTLYMLKKASGKNPLSDRLLEIQ
                     EVIKIGGLKGVRYFYQNRISRKQLVRTIGLYTIMLFGAYKKYIMKELL"
BASE COUNT          430 a          212 c          154 g          394 t
ORIGIN      
        1 tagttgatta atcattccac ttatttctac ttcatttgaa taataagata ggtacctata
       61 gttacttatc aaatctgtaa tattagaaaa acttctacct gaaaacaata ccactttctg
      121 aatttcacgg gtataaagaa atagcataac aagatttaga atcagaagaa tgacatagat
      181 tatctttgaa ggatttattt ctttcaattc ttcatacgaa attccctcca ctttaggcct
      241 gtacgacaat tttgtcaaca aggaaactat aataaatgta gcaaccccta acaatatcaa
      301 accaaaggtt ttgaaatgga gttctgttcc ccaaacattt ctattaacta gagcacttgc
      361 aatcgaaata aaataagtga gtgttaaaat tactgcaggt tgaaaaaaat ccccctcaaa
      421 taatataaat gtaatgataa ttaatagaaa aatagatatg aataagaaat ttaaaagcat
      481 tataagagct ctttcataat atatttttta taggccccaa aaagcatgat ggtatataag
      541 ccgattgttc ttacgagttg ctttcgagaa attcgattct gatagaaata tctcacacct
      601 tttaatcctc caattttgat tacttcctga atttcaagta gtctatcact taaaggattt
      661 tttccagaag ctttttttag catataaaga gtttgattat ataccctagc atgtgtaagt
      721 gccaattcat tgaaacccaa tatagccttt ctgaaaacat ttagcggaga taatttaaaa
      781 tgatgactag ttgttacatt atgtccatgt cttcgataca agaccagttg ttcagggcag
      841 aacaacacct tcccatactc tagcgcaaac tttgcaaaat aactatcatg agccagtttt
      901 tctatatata aataatcttt atcaatatca actgaaggaa ccatctctag caatgcatga
      961 ttaaaacctg cagtacaccc ccagatatag gcttgaataa aatacaaatt atttatgttc
     1021 ggtaattcaa tccccattat tttatttata ctaatatcta tcaaattatt actggcatca
     1081 atcgttgaca tatcagagta aaccatctct ggcatgtcat ctttagaaaa tcttaacagc
     1141 tgtatttcta acttgtgctc tttccaaata tcatcttgat cacaaaaaaa
//
LOCUS       DAVKZB010000200         1185 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVKZB010000200 DAVKZB010000000
VERSION     DAVKZB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.200"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>1185
                     /gene="phtB"
                     /locus_tag="VXA80_002009"
     CDS             <1..>1185
                     /gene="phtB"
                     /locus_tag="VXA80_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV0354439.1"
                     /translation="LIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIARIIP
                     LRYRSNHWVPDSRPEQPSPQPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFE
                     ENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGTKKTDLPSSDREFYNKAYDLLA
                     RIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVEDILAFLAPIRHPERLGKPNS
                     QITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEA
                     ERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTV
                     EVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDN
                     GF"
BASE COUNT          380 a          255 c          248 g          302 t
ORIGIN      
        1 gccttatttt cgacccagcg caaatcacaa gtcgaaccgc cagaggtgta gctgtccctc
       61 atggtaacca ttaccacttt atcccttatg aacaaatgtc tgaattggaa gaacgaattg
      121 ctcgtattat tccccttcgt tatcgttcaa accattgggt accagattca agaccagaac
      181 aaccaagtcc acaacctagt ccaagtccgc aacctgcacc aaatcctcaa ccagctccaa
      241 gcaatccaat tgatgagaaa ttggtcaaag aagctgttcg aaaagtaggc gatggttatg
      301 tctttgagga gaatggagtt tctcgttata tcccagccaa ggatctttca gcagaaacag
      361 cagcaggcat tgatagcaaa ctggccaagc aggaaagttt atctcataag ctaggaacta
      421 agaaaactga cctcccatct agtgatcgag aattttacaa taaggcttat gacttactag
      481 caagaattca ccaagattta cttgataata aaggtcgaca agttgatttt gaggctttgg
      541 ataacctgtt ggaacgactc aaggatgtct caagtgataa agtcaagtta gtggaagata
      601 ttcttgcctt cttagctccg attcgtcatc cagaacgttt aggaaaacca aattcgcaaa
      661 ttacctacac tgatgatgag attcaagtag ccaagttggc aggcaagtac acaacagaag
      721 acggttatat ctttgatcct cgtgatataa ccagtgatga gggggatgcc tatgtaactc
      781 cacatatgac ccatagccac tggattaaaa aagatagttt gtctgaagct gagagagcgg
      841 cagcccaggc ttatgctaaa gagaaaggtt taacccctcc ttcgacagac catcaggatt
      901 caggaaatac tgaggcaaaa ggagcagaag ctatctacaa ccgcgtgaaa gcagctaaga
      961 aggtgccact tgatcgtatg ccttacaatc ttcaatatac tgtagaagtc aaaaacggta
     1021 gtttaatcat acctcattat gaccattacc ataacatcaa atttgagtgg tttgacgaag
     1081 gcctttatga ggcacctaag gggtatagtc ttgaggatct tttggcgact gtcaagtact
     1141 atgtcgaaca tccaaacgaa cgtccgcatt cagataatgg ttttg
//
LOCUS       DAVKZB010000201         1175 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVKZB010000201 DAVKZB010000000
VERSION     DAVKZB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.201"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..438
                     /locus_tag="VXA80_002010"
     CDS             <1..438
                     /locus_tag="VXA80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354440.1"
                     /translation="PSLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGT
                     LSGGEQQKVAIARAVIADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTH
                     DIELASKTDRALILKDGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            441..>1175
                     /locus_tag="VXA80_002011"
     CDS             441..>1175
                     /locus_tag="VXA80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0354441.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQMSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLTGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQ"
BASE COUNT          363 a          158 c          234 g          420 t
ORIGIN      
        1 ccttccctgc cggtaataga aaatatagca cttcctttgc gattatcaca aaaaaaatta
       61 actattaaaa atgtagaaaa cttactcaaa agaatgaagt ttaatgctgg cttaaacgat
      121 tttgttggaa ctctgtctgg tggagaacaa cagaaggttg ctatagctag agcggttatt
      181 gctgatagtg atataatatt tgctgatgag ccaactgggg ctttagacag cgtttctcgt
      241 gaagtaattt ttgaattatt gagagagtta gtaggggcgg gtaagtgtgt aattatggta
      301 acgcacgata tagaattggc ctcgaaaact gatcgtgcat taatattgaa agatggaaaa
      361 attttcaaag aacttcatag acctagcggg gaagagttgt ataaaatctt agaggtacaa
      421 tcaactacgg aggaatagag atgctcagat tagtttacta tcaattttta cataataaga
      481 aacaatggtt aggcgtttca ccagtaattt ttgtttcgag tttagttatg ggattggctg
      541 ttaatggggt aattaatgtt gagaataatt cgcaagtatt tgtcggactt ccagatccta
      601 aaccaatttt tatgtttccg atagtattcg gaggagtaac attattcttt gtgctctcta
      661 acataataaa tatgttggtt gaaattttta gagatgatta cgaacttctg gaggtgttgg
      721 gagcaagtcg ccttcaacta tcatttcttg tggggggaca gatttttatc atttcctcga
      781 ttattagttt tattgcatat ctatgctcta tatttgttac gtcaaattat tattattttt
      841 tacaatactt tttcggggaa aatatattac ctgatatcca atttcaaatg agtgctgttg
      901 gatgtatcat tacagtagtg ttgatttcat ttttagcctt tctaagtggt tgcttttata
      961 cgttcaagaa gattagaaat aggaagagtt ctaaaataag gcatgttctt tcaatagtaa
     1021 aaagaatact gctattgact ggttttagtg ttatctggtt gctttctttg caacaaattt
     1081 tccaagatag cacaatttta gcgaaggccc agataatatt caatattgta atcttagata
     1141 ttgtgattat ttatcaatta tctcctttta tacag
//
LOCUS       DAVKZB010000202         1163 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVKZB010000202 DAVKZB010000000
VERSION     DAVKZB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.202"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            34..342
                     /locus_tag="VXA80_002012"
     CDS             34..342
                     /locus_tag="VXA80_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0354442.1"
                     /translation="MERQTLKDIEVIIVNQSTEEIASISQQMNLSFNIKIIDENSFAE
                     LSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGHFADLVDGVFYFYPWGG
                     "
     gene            347..>1163
                     /locus_tag="VXA80_002013"
     CDS             347..>1163
                     /locus_tag="VXA80_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEV0354443.1"
                     /translation="MTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLRKINQF
                     DINNLIWRLYIHSKTATYINFPTYIYKPVIDAKPLKDSYKIILENYENRIKDCAVLEN
                     YDIERAKKQYIQELANFSAWLKNDGEYLDSDIVYHKLIAAEKGIFPFLELDRLDFTII
                     SNNCVGGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFEEELISPNWT
                     HEVYPLGHLGDIDIHFLHYSSIEEARTKWEKRKKRINWNNLYFKFDNKDSASEE"
BASE COUNT          422 a          139 c          194 g          408 t
ORIGIN      
        1 gataaaaata gtttattaga gtctttgtca atagtggaga gacaaacatt aaaagatata
       61 gaagttatta ttgtaaatca atctacagag gagatagctt ccattagcca acagatgaat
      121 ctatcattta atattaagat tatagatgag aattcttttg ctgagttgtc tgatatgatt
      181 acaggagatt atattacgtt tctatcttca aatgattcat tatttgattg gacatttgaa
      241 aagatgtatc atgcgataaa attatccgat tctgatgttg ttcttggtca ttttgctgat
      301 ttggttgatg gagtatttta cttttatcct tgggggggat aattggttga cagaaaattt
      361 aacgaatgtt caagttttac aaaagatgga tgatacagat catcgaatta gaaaacaata
      421 tcgctcatta tttggaaaat tattcaatag taagctttta cgaaaaataa atcaatttga
      481 tataaacaac cttatttgga gactttatat tcactctaaa actgcaacct atattaattt
      541 cccgacatat atctataaac ctgttataga tgctaaacct ctaaaagact cctacaaaat
      601 catattagaa aattatgaaa atagaatcaa agattgcgcc gtcttagaaa actatgatat
      661 agaaagagcg aaaaaacaat atattcaaga attggcaaat tttagtgctt ggttaaaaaa
      721 tgacggtgaa tatttagatt ctgatattgt ttatcataag ttaatagctg cagagaaagg
      781 aattttccct tttcttgagc ttgatagatt agattttacg attataagta ataattgtgt
      841 tggaggatta atttataaac agcttggttt tcaatataga acaccttttg ttggtttatt
      901 tattcttcct gatgattatt ataaacttac aaagcatttt cgttattata tggaacaaga
      961 acttgttttc gaagaggaac ttatttcacc gaactggact catgaagttt atcctttggg
     1021 gcacttagga gatatagata ttcattttct acattattct agtattgaag aagcacgaac
     1081 taagtgggaa aaaagaaaaa aacgcataaa ttggaataat ctttatttta aatttgataa
     1141 taaagattca gctagtgaag aaa
//
LOCUS       DAVKZB010000203         1154 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVKZB010000203 DAVKZB010000000
VERSION     DAVKZB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.203"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..476
                     /locus_tag="VXA80_002014"
     CDS             <1..476
                     /locus_tag="VXA80_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354444.1"
                     /translation="KKLVRPSDISQENIVIFPYNSKNRPILEFKKKFPLTYRYLEEAL
                     SEEYKKNYGIFFGRTQGFLGYHSSKVIIPKVADLKNSPFKLINEGFVQSGLSITFFEN
                     YSNRVLTRIVDYLNSPTVLNYLSNISKNYAAGYQNISSTDLKYIKIPRRLLEDDV"
     gene            473..>1154
                     /locus_tag="VXA80_002015"
     CDS             473..>1154
                     /locus_tag="VXA80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0354445.1"
                     /translation="MKETLKYIVEDKVLAEVLGRNNFSTKESAVLELVKNAYDAGAEK
                     LTISFRKSKSTGKLFIEIVDDGIGMSSEDIKNAWMHVGKSTRDYIDSKTGRVFAGSKG
                     IGRFALARLGEVIELRSHKEGNSKIKWTTDWEKSKLESIESTNDEQGTIIRIYDLRDK
                     WSSRNIDPLKDYLSKVYYDNQMEITLDYRTDEIAITNKTENIWANPKIGENFVDSIEI
                     NYDSSTQKL"
BASE COUNT          447 a          142 c          209 g          356 t
ORIGIN      
        1 ttaagaaact agtccgcccc tctgatatta gccaagaaaa tatagttatt ttcccgtaca
       61 attcaaaaaa tagaccaata ttagaattta aaaagaagtt tccgctaact tatagatatt
      121 tagaagaggc actatcagaa gagtataaaa aaaattacgg aatttttttt ggaagaactc
      181 aaggattttt aggatatcat agcagtaaag taattattcc aaaagtggct gatttgaaaa
      241 actcaccatt taaactcatt aacgagggat ttgttcagtc aggtctatca attacatttt
      301 ttgaaaatta tagtaatcga gtgttgacta gaatagtaga ctatttgaat tcaccaactg
      361 ttttgaacta cttatctaat atttctaaaa actatgcagc gggttatcaa aatattagct
      421 ctaccgattt aaaatatatt aaaatcccaa gaagattatt ggaggatgat gtatgaagga
      481 gacattaaaa tatattgttg aagataaagt cttggctgaa gtgttaggaa gaaataactt
      541 ttccacaaag gaatcagctg tcttagagtt agtaaaaaat gcctatgatg caggtgctga
      601 aaaattaact atttcattta gaaaatccaa aagtaccgga aaacttttta ttgaaattgt
      661 cgatgatgga attggtatga gtagtgagga catcaaaaat gcttggatgc atgttggtaa
      721 gtctactaga gattacatag attccaaaac tggaagagtc tttgctggtt ctaaaggaat
      781 agggagattt gcacttgcta gattaggtga agtaattgaa ctacgttctc ataaagaagg
      841 aaattctaag attaaatgga ctacagactg ggaaaaatct aaactggaaa gtatagaatc
      901 aactaatgat gaacagggga caataattag aatttatgat ctcagagata aatggtcatc
      961 tagaaatatt gatccattaa aggattattt gagtaaagtt tattacgata atcaaatgga
     1021 aattaccctt gattatagaa cagatgaaat tgctattaca aataaaacag agaacatatg
     1081 ggcaaatcct aaaattggcg agaattttgt tgattctatt gaaattaatt atgattctag
     1141 tactcaaaaa ttaa
//
LOCUS       DAVKZB010000204         1145 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVKZB010000204 DAVKZB010000000
VERSION     DAVKZB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.204"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            28..381
                     /locus_tag="VXA80_002016"
     CDS             28..381
                     /locus_tag="VXA80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0354446.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQV
                     NQNLEKIRQREGDTH"
     gene            596..712
                     /locus_tag="VXA80_002017"
     CDS             596..712
                     /locus_tag="VXA80_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0354447.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            705..938
                     /locus_tag="VXA80_002018"
     CDS             705..938
                     /locus_tag="VXA80_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354448.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT          377 a          223 c          245 g          300 t
ORIGIN      
        1 tcaattcact atagaaggag gaaaaggatg attctcagac atctgggaat tagcccgacc
       61 aatgacttgg ttgctaagaa aatattcagc aatccagaaa tcacttgtca atttattcgc
      121 gatatgctgg acttgccagc aaaaaatgtg accattttgg agggaagcga tattcacgta
      181 ttactctcca tgccttactc ggtgcaggat ttttatacca gtatagacgt cttggcggag
      241 ttggataacg gtactcaagt aattattgag attcaagtcc atcatcagaa ttttttcatc
      301 aatcacttgt ggacttacct gtgcagtcag gttaatcaaa atcttgaaaa aattcgtcag
      361 cgagaaggtg atactcacta gagctacaaa cacatcgctc ctgtttacgc cattgctatc
      421 gtggatagta attatttctc agatgacctg gcttttcata gctttagtat gcgcgaagac
      481 acaacaggtg aggtattggc gattaccaac aatggacagg aaaaccatct ggttaagatg
      541 gcattcttgg aattaaaaaa tacagagaaa ccagcaaaga caaggttcgc aagccatggt
      601 tggagttttt cggcaacaag ccctttaccc agcaaccgca acgagccatt acccaagcaa
      661 atcaactgct ggactacaag agctggtccg aggaggacag gaaaatgttt agtcaactac
      721 atatgcgaga agaacaagtc ttgttagcac aggactatgc cttggaaact gctagggctg
      781 aaggccttga acaaggacta gagcgtggga aagttgaagg aagggcagaa aggaaacttt
      841 ttgccttcct agacatagta cgccaaggtc ttctgacttc tgaggttgcc agccagcaat
      901 taggtatgtc agtatctgaa tttgaggcac tgttgtaaaa tggctccata atatccatag
      961 tgggtaaatc ccctatggat attatggagc ctattttgtg tagaaaaaaa gtcccatatg
     1021 acctataatg aaaagcgaca aaacaactca ttagaaagaa tcatgtggaa caattacatt
     1081 ttatcacaaa attactagac attaaagacc ctaatgtcca gattttaaac atcatcaata
     1141 aggat
//
LOCUS       DAVKZB010000205         1139 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVKZB010000205 DAVKZB010000000
VERSION     DAVKZB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.205"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..605)
                     /locus_tag="VXA80_002019"
     CDS             complement(<1..605)
                     /locus_tag="VXA80_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0354449.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHS"
     gene            complement(676..975)
                     /locus_tag="VXA80_002020"
                     /pseudo
     CDS             complement(676..975)
                     /locus_tag="VXA80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT          370 a          225 c          181 g          363 t
ORIGIN      
        1 aaagaatgcc ccatgatgga taagagagtc tgaccagaaa agagttttat acctgctgct
       61 ctcatttcct taatccgagt gataatcact aaagcaaaga aagataagcc aaatattgct
      121 aaactaatta aaataagggg atttagtaat attcgaaaag caagaaaata gggcggtatc
      181 tttcggtcag cacttgcttt ataacccaaa tctcctaatt tatcggcaag cttttctttc
      241 gtcaaggagc ctgacaaaag gagataacta tttagcggat cactacgttc acgactttct
      301 tggctagctt cttggaattc ttttggtaaa gttccctgac cataagttgc ataagtaaag
      361 tgagtcgtcc catccttact cggctctaca attcttctag ctattaaact ctgttctgag
      421 tttgcaaaat tctccaattc ctgttcaaat acctcacgcg tcggttcctg agtatctttt
      481 ttgacacgaa gtaaagaaac ggaatcatag cttgcatata aatattgtgg cgcacgtaag
      541 acaataatcc aagcaaggaa gaagctgaga aaaaaagttg ataataatat gaatagtttc
      601 ttcatagtag actccttgta aacaaaattc cccctgtaat ttcttacaag gggaacgatt
      661 taaatcaatg aacgattagt cataatcaca gtaaaatgct acttgttctc cccatttagt
      721 ccaaatccat gcataggagg tttttccagc aggagcttct cctttatcag aatgaccatc
      781 ccatctgctc acgatggatg aataatgata ttttttacca tgatagtaat ttgaaaaagc
      841 ctaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat attgtacagc
      901 agctgatgca gctcccaata atgtaaaatt tgaaataaga gctagagcaa gtaatctatg
      961 ttttttcgtt ttcattttat tttttctttc aaaaaaagca caccttgagc aacaatgcaa
     1021 caaaataaat cctcctctct cttttattga aaccgctttc ttatgtgata agaataactt
     1081 ttttattatt tgttgtcaag gaaaaaatcg aattttttag atattttact atattacct
//
LOCUS       DAVKZB010000206         1088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVKZB010000206 DAVKZB010000000
VERSION     DAVKZB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.206"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(180..899)
                     /locus_tag="VXA80_002021"
                     /pseudo
     CDS             complement(180..899)
                     /locus_tag="VXA80_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          372 a          196 c          119 g          401 t
ORIGIN      
        1 atagctacta taaaatctaa tcctaaatca cctggaatac ccaatttact tactatgact
       61 agtaacacaa tgccattcaa agcataaata atggaaactg agatggcata tagaaaatta
      121 gacaagacgt aaatcatccg attcttctgt ttatttaatt tcatgattat aaccttcctt
      181 tattttgaac gatttaggta aatatcttta agcgtctctt ttttagtttc aaaatcaact
      241 actttaatag agttttcgtt aaaaaattta aaaagctcac tattttgaat attcccagac
      301 atagtaattc tcaatccctc ttcctaaaca atatcccaaa attcttgttt ggtaatgaaa
      361 atgtctctat ctgtagctga tgaaaaagct atctcaaaca agaaattatg actatctttt
      421 cctacttttt gaaatgtcaa aagcccgttc tccaagaaaa gaaccctctc acaaatttct
      481 tcaatatctt ctaatttatg actcgatatt aaaattccca cattttcatg taaagctaaa
      541 ttttttagaa ccgataaaac tatttgtgat gattcaatat ctaaaccatt agtcggttca
      601 tctaaaatca atatatcagg ttccgtaacg agagttagaa gcaaagctaa tttttgtttt
      661 gtacccaaag aataggtctt tactttttta ttaatagact gagtcaaatc taactcttgg
      721 atcaaacttc taaatctttc ttgattgtag tcaacaccat ataaatttga caaatatttt
      781 aaattctcta aacctgtttt agataaaaat aattttggtt cttcgattaa atacccaaca
      841 ttatcactgc ttataatatt acctgaagtc ggttgattat tctgaacaag aattttcatc
      901 aacgtactct taccaactcc atttctccct acttgaccaa caatttcacc cttgttaagt
      961 gcaaaattta tatctttgag aatgattgaa tgaccataat gcttacttac atttataact
     1021 tacatttttt attcctcaat taatattcat ggtttgctct ttgattctta ttgagtttaa
     1081 aaagttgc
//
LOCUS       DAVKZB010000207         1045 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVKZB010000207 DAVKZB010000000
VERSION     DAVKZB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.207"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            40..702
                     /locus_tag="VXA80_002022"
     CDS             40..702
                     /locus_tag="VXA80_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0354450.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          345 a          164 c          190 g          346 t
ORIGIN      
        1 aatgagattc gtgaaaagat tctttgtaat aaatatacca tgaacgaaca attgcctgat
       61 gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa agctttagac
      121 ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt tgttctctct
      181 cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg actgacaaaa
      241 atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa attagaattt
      301 gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt ttataatatt
      361 taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta tatgagtacc
      421 gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa ttacattgaa
      481 ggaaagttag gattgcatat tgccagtgct acaaaaatct tacgagcttc ttctagttca
      541 gaaaatgagc aacattactt gcagctcctt ccaacggaac cggtatttga agtagaacaa
      601 gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca tcgctatgat
      661 ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat gtgaaaatga
      721 agccaatctt ttacagactc tagtttaaga aaaatttaaa acagggcaag aaggttccat
      781 ctatgcttaa atggtttctc ttttctaaat aagatggctt taaaagagtg atcgttttat
      841 ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta ggtactgaaa ttgttcactt
      901 gatctacttc ttatagttat ttagttttaa atagtgtttc aaacattctt acactgacga
      961 gaagtttttg agtcttttct tgtaacacat atagtatact gaggttagaa tagtagactg
     1021 tgacttctaa aaaattgcta gaaat
//
LOCUS       DAVKZB010000208         1042 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVKZB010000208 DAVKZB010000000
VERSION     DAVKZB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.208"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..1042
                     /locus_tag="VXA80_002023"
                     /pseudo
     CDS             1..1042
                     /locus_tag="VXA80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          230 c          170 g          324 t
ORIGIN      
        1 ctggtatgcc tactagaatt ctccttagaa agcgtcgatt caagtgttat cactgttcaa
       61 aaatgatggt cgctgaaact tctatcgtca agaagaatca ccaaatccct cgtattatca
      121 accaaaaaat tgcgcaaaag ttaattgaaa agatttctat gaccgatatt gcccatcagc
      181 tttccatctc aacttcaact gttattcgta agctcaatga ttttcacttt aaacatgatt
      241 tttcttgtct tcctgagatt atgtcttggg atgagtatgc ttttacaaag ggaaagatga
      301 gtttcattgc gcaagatttt gataatctta atattatcac tgttcttgaa ggcagaacac
      361 aagctgtcat ccgaaatcac tttcttcgct acgatagagc cgtccgatgt cgcgtcaaaa
      421 tcattactat ggatatgttt agcccttact atgacttggc taaacagctt cgctttcaaa
      481 tttctaggct caggctgaaa cagtctccca gactgtttca ctcccgaatg ctaaaatcgt
      541 tcttgatcgc tttcacattg tacaacatct tagccgtgct atgagtcgtg tgcatgtcca
      601 aatcatgaat cagtttcatc gaaaatccca tgaatacaag gctatcaagc gctactggaa
      661 actcattcaa caggatagcc gtaaactgag tgataagcga ttttatcgtc ctacttttcg
      721 tatgcactta accaataaag agattttaaa caagcttttg agctattcgg aagacttgaa
      781 acaccactat cagctctatc agctcttgct ttttcacttc cagaacaagg aaccagagaa
      841 atttttcgga cttattgagg acaatcttaa gcaggttcat cctctttttc agactgtctt
      901 taaaaccttc ctcaaagata aagaaaagat tgtcaacgcc cttcaactac actattctaa
      961 tgccaaactg gaagcgacca ataatctcat caaacttatc aagcgcaatg cctttggttt
     1021 tcggaacttt gaaaacttca aa
//
LOCUS       DAVKZB010000209         1027 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVKZB010000209 DAVKZB010000000
VERSION     DAVKZB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.209"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..1000)
                     /locus_tag="VXA80_002024"
     CDS             complement(<1..1000)
                     /locus_tag="VXA80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0354451.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNI"
BASE COUNT          364 a          177 c          133 g          353 t
ORIGIN      
        1 aaatattttt tgcttcatat aacggaaaat ctctattcac ctcatttggt tcgatatcat
       61 tcattttatt tgactcaatt ggattaattg aatacaattt taaaaaaata ggcttcgtac
      121 caataataga ggataattga cctcctaatt cacctagcgc tgtaaaaata acacctgtta
      181 gtgctcctat tgcttcaata gtaccaattt tcactattcc ttttattgca agatagcctg
      241 ttaaaaaaac gagagatatc tgaaaaaaaa tattgagaaa gaagctaata gcgcctgcta
      301 acgtttctac agttgtcttt ctttgtataa ccatctttaa taaaattcct gcttctttaa
      361 ttttcttagg caatacatat aaaagattca aggacgctaa cacatcaaat ccattcaata
      421 tagtctcact agattttaaa aaagcttcat tttggttagt taaatttaga ctaacttctc
      481 gcattttcga tgcaaagatt tttggtacaa gtagcataat cattaatgaa aacaaggtgg
      541 ctacagtcaa tgaccaatga tagtgattaa gagtcacaac tgcaaatata gtaccagaaa
      601 ttccttttat tactaaaaaa agttgtttaa aagcctgatc atttaaagtc tgaacgtcat
      661 tatttagcca cgaaagatat gttcctgatg atttactatg aaattcttga taggtagagt
      721 tagagatgtc tgtggcaact ctatttcgaa tctctagatt aaactcttgg atcacttcaa
      781 cctgataatt tttcactacc cagtcaagga atattatccc acaccagaca atcatttggt
      841 agattgacaa tttcaaaaac cgctctaaat tcatcgcaat taattcattc aacaccagag
      901 cattaatagt tgctgcataa attagcaata attgaccagc aacaataaat atcgttaata
      961 aactaaattt ttttatattt gattttataa tagtatacac aatagtttct cactttctaa
     1021 attttaa
//
LOCUS       DAVKZB010000210         1015 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVKZB010000210 DAVKZB010000000
VERSION     DAVKZB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.210"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..363)
                     /locus_tag="VXA80_002025"
     CDS             complement(<1..363)
                     /locus_tag="VXA80_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354452.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYR"
BASE COUNT          432 a          155 c          125 g          303 t
ORIGIN      
        1 tcggtataaa agatactgac tcataccttg ataccaaata aaattcggta tttcaataag
       61 agcatgaggt aatattcctt ttattgtagc tactattcca atcatctgac cagatatacc
      121 caattgtatt cctaaagata gcaaatcaaa aagttgcaat aagggcgaaa tcggaaaaca
      181 gaatagatat agcaaaaaat ttgtagaatt tgcctgtaaa actgtcaatg taaattgtaa
      241 ttccagtttc ggaggtatag tagcaaaagt ctcatttttt aatataaagc ttaaaagtaa
      301 acctagtaaa aaacagacta tacaaaataa cacatagtaa atcagaaaac cactttttct
      361 catctaatgc cccctactaa aattacaaat attatcagaa aacaatataa aaccagaaaa
      421 ctccatctca ataaactaac tgtaccttta attaaaaata gagctttctt ataatagaca
      481 taggctataa aataattgaa tggaatcaat aacaaattca acaaaatact ttttatatca
      541 ttactataac ttgtcaccgg aatcaaacat aacaacaatg aaaattgtaa gaaagagatt
      601 aaatgcgtca acatcattag ttgtgcattc ttaaaaaaga aattcatttt agaatgatta
      661 agagcagata attgaagtaa agctagatct accccaattg aggatttatg attccctatt
      721 attgcctcca ttaatgtaat atatattgag gtataaagaa ataatattgg tatttttgtg
      781 tcatcaattg ataacatcgt catcatatac caaaatagat aggaaagaag aatactaatc
      841 aaaataaaag aaaaagttaa ccctttattt aaccaacaaa aacgaagcaa tataaataga
      901 ctgctacgaa atcttttttg actcattcga ctgcttttaa aatagaaatt actcgcatga
      961 ttatctaata tccccatgac taaaataaaa taattgagta gtatgacata aatta
//
LOCUS       DAVKZB010000211         1014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVKZB010000211 DAVKZB010000000
VERSION     DAVKZB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.211"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(26..>1014)
                     /locus_tag="VXA80_002026"
     CDS             complement(26..>1014)
                     /locus_tag="VXA80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0354453.1"
                     /translation="YYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYWFY
                     EGGSFIIDIHDFYIKEEVVQNSTYSMLDYVSIYSSYIVSANGEKFNPYQTITANSLCT
                     FDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQIIL
                     TKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKALED
                     VARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETLLL
                     NTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          331 a          164 c          129 g          390 t
ORIGIN      
        1 aaaacatggc ttgtaaaaaa ttactttaat cacaataatc gcatttaaaa ttgtgatgtt
       61 tgcaagctaa attacggact tcacttggaa gttttccctt gtatctttta taatagatag
      121 aaaatttgct ggcagatgaa tatccaacag attctgctat ctcttttata ggtagttcag
      181 tgtttaaaag aagagtttca gctacattca ttctttttct ttgagtgtac tctgtaatgc
      241 tttgacaata tttttcctta aataaatttt ttaatttagt accactcatt ttagatattt
      301 tttcaagcgt gccttgattt acattcgttg caaaatgatc atctaagaat cttgctacat
      361 cttcaagtgc tttatcatca tcaatttcaa ttttatattt ttttctattt aagtatgtgt
      421 caattactat acttatccat tcatttgcct ttgctttaaa gaaaaaatca gcggcaggag
      481 cgtccatctt acaatttaat atttccattg ccactctttc taaggccttt gtaagtatta
      541 tttgattcgg ttgaagcaag gttgaataaa aagattctgg attaatgtta atagatgcta
      601 aatgtttttc tattagctct tttttaaaac caatggaaac agcaagataa caacaattct
      661 cgtgtaataa aaaaacaaaa tcatctttta tattatcaaa atcaaaagta cataaagagt
      721 ttgcggtaat agtttgatac ggattaaact tttctccgtt tgcactgaca atgtaacttg
      781 aataaattga aacatagtct aacatactat aagtgctatt ttgaactact tcctctttga
      841 tataaaaatc atgtatatcg ataatgaagc tgcctccttc ataaaaccaa tataggcctt
      901 ctgcataagt tgaattctct tttttccaac aaaatgtatg acctgcactt gaatacttct
      961 cattgttttt acattcatct actttcatat aatcttttac aaaatcataa tatg
//
LOCUS       DAVKZB010000212         1014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVKZB010000212 DAVKZB010000000
VERSION     DAVKZB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.212"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            28..507
                     /gene="rlmH"
                     /locus_tag="VXA80_002027"
     CDS             28..507
                     /gene="rlmH"
                     /locus_tag="VXA80_002027"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV0354454.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            551..624
                     /locus_tag="VXA80_002028"
     tRNA            551..624
                     /locus_tag="VXA80_002028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:585..587,aa:Arg,seq:tct)
     gene            789..914
                     /gene="comC"
                     /locus_tag="VXA80_002029"
     CDS             789..914
                     /gene="comC"
                     /locus_tag="VXA80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV0354455.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
BASE COUNT          359 a          129 c          199 g          327 t
ORIGIN      
        1 aaatagagaa tatgttagaa tagagtcatg aaaattaaag ttgtaacagt tgggaaactg
       61 aaagaaaagt atttaaaaga tggtatcgca gagtattcaa aacgaatttc tagatttgct
      121 aagtttgaaa tgattgagtt atcagatgaa aaaacaccag ataaggccag tgaatcagaa
      181 aatcaaaaga ttttagaaat agaaggtcag agaattttat caaaaattgc tgaccgtgat
      241 ttcgttattg tgttagccat tgaagggaaa actttcttct cagaagaatt tagtaagcag
      301 ttagaagaaa cttctataaa aggattttct actcttactt ttattattgg gggaagttta
      361 ggattatcat catctgtaaa aaatagagcc aatctttctg tcagttttgg tcgcctaacc
      421 ttaccccatc agttaatgag actagttctt gttgaacaaa tctatcgcgc ttttacgatt
      481 cagcagggat tcccctacca taaatagaaa attgactttt aattgaattt ttggtagaat
      541 aattgtatta ggtctcatag ctcagctgga tagagcattc gccttctaag cgaacggtcg
      601 caggttcgaa tcctgctggg atcaatataa tagcaaagct gggaattttc ccggcttttt
      661 tcttaaaaaa gtacactttg ggagaaaaaa atgacagttg agagaatttt atctaaaacg
      721 aaattccatt ttgtataatg gtttttgtaa gttagcttac aagaaaaaac attttaggag
      781 attttattat gaaaaacaca gttaaattgg aacagtttgt agctttgaag gaaaaagact
      841 tacaaaagat taaaggtggg gagatgagga tttcaagaat catccttgat tttcttttcc
      901 tacgaaaaaa gtaatgaaat aaggagaaag agtaatggat ttacttggat ttgggacagt
      961 tattgttcat tttttaatta ttagtcacag ttaccgttta atttgtaaag gtcg
//
LOCUS       DAVKZB010000213          999 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVKZB010000213 DAVKZB010000000
VERSION     DAVKZB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.213"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            164..961
                     /locus_tag="VXA80_002030"
     CDS             164..961
                     /locus_tag="VXA80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV0354456.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          266 a          168 c          214 g          351 t
ORIGIN      
        1 gaatcgttca ggacagtcaa atcgatttct aacaatgttt tataagtaga ggtgtactat
       61 tctagtttca atctactata aacttttatg aaatacagaa aacgcttcct aaaacctaaa
      121 gtttgtgata taattatcaa gaaaaaagat ttaggagttt attatggttt cttcagaatt
      181 tatttcaaag attgaatttg cttgcaataa gaaagaaagt ctttatagtc aaagcaaatt
      241 taagtatgcg attcgttcga tgttcgcagg tgcattttta accttcagta ctgctgcagg
      301 tgcagttggg gctgacttga ttaataaaat tgcaccaggt agtggacgct tcctctttcc
      361 attcgttttt gcttggggct tggcctacat tgtttttttg aatgccgagt tggtcacttc
      421 aaacatgatg ttcttgactg ctggtagttt cttaaaaaaa atctcttgga gaaaaacagc
      481 tgagatttta ctatactgta ccttgttcaa ccttatcgga gccttgatag cagggtgggg
      541 ctttgctcat tcggcagcct atgcgaatct gacacacgat agtttcatct caggtgttgt
      601 tgagatgaag ttaggccgct caaatgaatt ggttttgctt gaggcgattt tggcaaatat
      661 tttcgtaaat attgcgattc tgtcatttat tttggtcaaa gatggtggtg ccaaactttg
      721 gcttgtgttg tcagctattt acatgtttgt attcttaaca aacgagcaca ttgcggcgaa
      781 ctttgcttct ttcgcgattg tgaaattcag tgttgctgcg gattcaattg ccaacttcgg
      841 tgttggaaat atgcttcgcc actggggtgt gactttcatc ggaaacttta tcggaggagg
      901 cctcttgatg ggtcttccat atgccttcct caataaaaac gaagatactt atgtagatta
      961 agaaaatgag cacgattgag tcgtgctttt ttcattttc
//
LOCUS       DAVKZB010000214          986 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVKZB010000214 DAVKZB010000000
VERSION     DAVKZB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.214"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            15..>986
                     /locus_tag="VXA80_002031"
     CDS             15..>986
                     /locus_tag="VXA80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEV0354457.1"
                     /translation="MANNRKTETLGVSYLSTFIDKHELLQSYFESNDKTPVWDGEIHV
                     LKSPSEKKDEILGKVPVQIKTTRQKKDVLKSFSLDTRDLELYKPNGGVVLFVVWLNED
                     NGLRDIYYKSLPPLSIKNLLKKSKLKNKSTNRKKLSIEIFKLDEKKMYPMLVDFINNS
                     QKQYSFINVEGISVEDIPDDKTLKFYFYGQEKEEIFNYQEEHDLFIYYLDPITGIEIP
                     LENTIKIVETEEETDLIIKIGDYVFQDVKRHRFPDGSVQLHFGESFTMSFDIKKKQFK
                     FNYTRPDLLSKAIKCTQVFQELGKIGYFTLNGNKIELDERSIKDISSL"
BASE COUNT          378 a          123 c          176 g          309 t
ORIGIN      
        1 ttagaggtgc agatttggcc aataatagga aaactgagac attaggtgtt tcatatcttt
       61 caacatttat agataagcat gagttacttc aatcttattt tgaaagtaat gataaaactc
      121 cagtatggga tggagaaatt catgttctta aatcaccgag tgagaagaaa gacgaaatat
      181 taggtaaagt acctgttcag attaaaacta ctagacagaa aaaagatgtg ttaaaatcct
      241 tctcattaga tactagagat ttagaactat ataaaccaaa tggtggagta gttttatttg
      301 tagtttggtt gaatgaggat aatggtttaa gagatattta ctataagtca ttacctcctc
      361 tttctataaa gaatttgctc aaaaagagta aactaaaaaa taaatctaca aataggaaga
      421 aactttccat agagattttt aaactggatg agaaaaaaat gtatccaatg ttagtggatt
      481 tcataaacaa tagtcagaag cagtatagtt tcataaatgt cgaaggtatt tcagttgagg
      541 acattcctga tgataaaact ctaaagttct acttctatgg tcaggaaaaa gaagaaattt
      601 ttaactatca ggaagaacat gatctcttta tttattattt agatcctata actggcatag
      661 aaatacctct tgaaaacaca ataaagattg tagaaactga agaagaaacg gatttaatta
      721 taaaaatagg cgattatgtt tttcaagacg taaaaagaca tcgttttcct gatgggagtg
      781 tacagcttca ttttggtgaa agctttacaa tgtcttttga cataaagaag aaacaattca
      841 aatttaacta tactaggccg gatttgctgt caaaagctat aaagtgtact caggtatttc
      901 aagagctagg aaaaatcgga tattttaccc ttaatggtaa taaaatagag ttagatgaac
      961 ggtcaataaa ggatatttct tcactt
//
LOCUS       DAVKZB010000215          956 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVKZB010000215 DAVKZB010000000
VERSION     DAVKZB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.215"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(90..539)
                     /locus_tag="VXA80_002032"
     CDS             complement(90..539)
                     /locus_tag="VXA80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0354458.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGLLVEEPINIKKVVFKVKI"
BASE COUNT          330 a          167 c          107 g          352 t
ORIGIN      
        1 atcgttaatc ggagatggac tagaatgctt tttgaaaaca gatgagtatt aaatatttta
       61 aaattcttaa tccctgaaag tactttcagt taaattttta ccttaaaaac aactttctta
      121 atattaattg gttcttcaac caataggcct gttttatgtg catcgtttgg ttccaacaaa
      181 agtaacatat ctttctttaa tttaatacta aataaagctg tcccattcat ttgttgccaa
      241 tcatcatcta ctttatattc agacaattcc atttgagaca agaaattata taaaattatt
      301 tcttctccat ttaacagata atggacatca taatattcct tatgactttc ccacggtcta
      361 ttttcttcag ttgtagtttc catatcaatt atattgtaaa agaaatcagt agaaatttta
      421 tgtatgccac ctttatcttt tggtatatca tgattattta gaaactcttt tactttcgtt
      481 attgcgatat gctcatgaac cgtattaaca tgtccatttt taaattcaac taattccata
      541 gttccctcct gttataaatt ttcttgatta aaactaactg gtagtagttc tttaagttta
      601 tatactttat aagtatcaac atcactagcc aatataacta caaatgaatc aagattacaa
      661 aattctatca ttacctgaca agacaccctg caagatgcta taaaagatga taaaatacct
      721 ttttttccgc ctacaataca tatttttgaa aacgaacgtt ctcctttaaa tacagctttg
      781 taaaaatata ctcgttctgc acagcaagta ggagaaaagc aacattttct atattacatc
      841 cagaaaaaaa tttttatctt cagtttctaa cgcagctcca accttaaaat aagaataagg
      901 agcataagct tggaccattg atttaataac ctctgatata attactttat aagtca
//
LOCUS       DAVKZB010000216          953 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVKZB010000216 DAVKZB010000000
VERSION     DAVKZB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.216"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(232..441)
                     /locus_tag="VXA80_002033"
     CDS             complement(232..441)
                     /locus_tag="VXA80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354459.1"
                     /translation="MFFAGIGEIHFLLFLLYLSMSASPIIGLVIGYTKINLINSIIIA
                     LGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          369 a          172 c          107 g          305 t
ORIGIN      
        1 aatcaatgga aaataaaata ttgcaactag aagcgaataa agtcccgcta ataaatattt
       61 ctgatcatct ttcatttaaa acaccatatt atttactaag aatgcatcaa tacaatagag
      121 caactctgta agtcaatatt tgataaagca aagctttaaa cccttcccca gacgttagct
      181 tttattcaac ccactacagt tgacaaggag cccaaattat tgataaaaac tttagtcatc
      241 atcgtctcta taaaaatatt ttctatacac tcttgataat accaaaatta atgccaatat
      301 cataccaaga gctataataa tagaatttat tagatttatt ttagtatatc caatcactag
      361 tccaataatt ggactagcag acatgcttag atataataaa aataacaaaa aatgaatttc
      421 accaattcct gcaaaaaaaa tgtttcatag ctatagtttt acgcccataa atacccatca
      481 aatttaccgt ctacgatatc taaaccatat gcaatggctc ctcctaatga gaagacttca
      541 gcaacagtag ccgcaatatt aagagcatta gtaatagttg ccatactaac tccaccaata
      601 tatcttgtta tagcacttag ttacctagat aagatatcct ttagcttgct tccaaaattt
      661 aagtctgcac ctataatatg agttcatatt agtactgcta aatcctatct atagtaatgt
      721 caaacaccta aataaagtca tctctccaat tcattcaaga aatgaagaaa ggcaaaaaat
      781 ctttaaaata agaaaaatgc atattatcag gtgttcaaaa atattgatga tatacgttat
      841 atagatagaa agatttactc aatctcattt tcaaattgag ttttttccca acctcgtttt
      901 taatttatat cttcaataaa atctaatgac ctagttgata tgttatatgg aat
//
LOCUS       DAVKZB010000217          934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVKZB010000217 DAVKZB010000000
VERSION     DAVKZB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.217"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..827
                     /locus_tag="VXA80_002034"
     CDS             <1..827
                     /locus_tag="VXA80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0354460.1"
                     /translation="KNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWTYSMGDAFICVS
                     ETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIPNNKKIICYVGRLD
                     PEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPA
                     TIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDGFLFEKED
                     VEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG"
     gene            820..>934
                     /locus_tag="VXA80_002035"
     CDS             820..>934
                     /locus_tag="VXA80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0354461.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNI"
BASE COUNT          343 a           84 c          198 g          309 t
ORIGIN      
        1 acaagaatgg atttggaaag gttaagacag tgataacatg ccatggatgg gttgaatata
       61 acttaaaaaa gaaattcttg acttattttg atttttggac atattctatg ggagatgctt
      121 ttatttgtgt atctgaaact atgaaaaaaa gattggagag tataataaaa aataagaaga
      181 tagttgcaat aaataacgga attaatgtat caaatagcga tttagatgta gtaggtgttc
      241 aagactttaa aaaagaattt tgtattccaa ataacaaaaa aatcatttgt tatgttggaa
      301 ggttggatcc agaaaaaagg ccggatagat tccttgaatt tgcagaaaaa ttatttttag
      361 tcagagaaga tgtgattttt attatggctg gaaatggtag tatgtgggct gctttgaaag
      421 aaaaaatttg ccatttgaaa tgtagagata atttcaggtt acttggtgaa atctatccag
      481 ctactatagt ttaccaaata tcggatttat tgtatattcc ttctgataca gaaggaatac
      541 ctatgtgtgt tttagagtca atgtcacaag gaactccggt tttagctagt aatgttggcg
      601 ggttaagtga aattattgaa catagggttg atggattttt atttgagaag gaagatgttg
      661 agggagtgtg tgcttgtgct aattttttac tcaatgattc tgagtatttg aaatatatag
      721 gtgagaatag taaatcaaaa ataagaaaac atttttctgt gcaaaaaatg tttgtagaaa
      781 ccatgagagt atatgatgaa ttattagaga agagtagtca tggataggaa tatagatcaa
      841 gaattagtaa gtattataat accaacacat aataggtatg aatctctgat tagagctgtg
      901 aaaagttgtt tacatcaaag ttataaaaat atcg
//
LOCUS       DAVKZB010000218          929 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVKZB010000218 DAVKZB010000000
VERSION     DAVKZB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.218"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            205..543
                     /locus_tag="VXA80_002036"
     CDS             205..543
                     /locus_tag="VXA80_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV0354462.1"
                     /translation="MLKKYPCTIQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKIKGTRITK"
     gene            569..>929
                     /locus_tag="VXA80_002037"
     CDS             569..>929
                     /locus_tag="VXA80_002037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354463.1"
                     /translation="MMYLDGGGYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLIN
                     YIKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLD
                     LMVYIFLGYVLVIQKDVY"
BASE COUNT          308 a          114 c          206 g          301 t
ORIGIN      
        1 tcgatataat gtaagggagc ttgagttgtt aaaggaggct tagtccttgg acaaaaatct
       61 gctgtactac aatgcttgga aagatatcaa gaacaaagaa tgatacgatt gaaactaaac
      121 tgattcaaga actactgggc agtgattgag gtttgggtga catcaaagaa aagttagatt
      181 aattctaaag aattgagtat ttctatgtta aaaaaatatc cttgtacgat tcaacatgat
      241 cagtcagatt gtgctgcggc agttgtttca acagttcttt tatcttacaa aaaggaacta
      301 tcaatcatga agattcggga aattattggt acagacatgt atggaacgac tgtcagtggt
      361 attgtttcag gtctgaataa gttgaatttt acagtaaaag ctgttcgagt agcactggaa
      421 gatttgactc caaaattaac atttcctgcg attcttcaag ttaagaatga tttaggtcaa
      481 aatcattttg tggtattaca tagtataaag gagaaaataa agggaactcg tattaccaaa
      541 taattatgtt gctcttgagc aagaggagat gatgtatctt gatggggggg gttatttaag
      601 caagagtgct tgtcaaggaa tttgcgcagc tttagctatg agtccaggaa cttttatagc
      661 attgactgga gctgcagttt taacaaaaaa actaataaac tatattaagg ttggaggcct
      721 tggaggttgg cttattggtg cagcagcagg tgtattggct cttatctttt tgataaaaat
      781 ttgtgtagat actacgataa aggttctatt aaacagaatc tttaaggcaa gtaaggtaat
      841 gaaaaggagg atattttctc tttttgtact agatttgatg gtctatatat ttttaggtta
      901 tgttttagtg atacaaaaag acgtatatc
//
LOCUS       DAVKZB010000219          902 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVKZB010000219 DAVKZB010000000
VERSION     DAVKZB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.219"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>902
                     /locus_tag="VXA80_002038"
     CDS             <1..>902
                     /locus_tag="VXA80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0354464.1"
                     /translation="LYRNAFSIDSFEGRRDWLKLSQRAVSSPAAATHPNGSWRVRPNQ
                     VSGHISIDKRKNKYIEDISNRQGVVENIYYHIFLEIIHIALKEFESYRQGIIRTINEY
                     QTALLEKAIYNEQREITAEEIVRDTIKNPDRITELTKTDFEKIQEKLNVQRKVIDNLG
                     EDRKRIEEDYRYEVQLLNVLSTLQLKVSSLSHEVKNNRNSIAANPTKIQEVLKRKYDW
                     DALKLDKPSSRNLPLLIETLSNDLEKVLDLADTIIDETKKDNFVVKEFKLDEITGLII
                     NKWKQQYNWVNFIIKVEPDEKITI"
BASE COUNT          357 a          119 c          167 g          259 t
ORIGIN      
        1 tttatatcga aatgccttta gtatcgattc atttgaaggg agacgagatt ggttgaagct
       61 atcacaaaga gctgtatctt cacctgcagc agctactcat ccaaatggat cgtggagagt
      121 gagacctaat caagtttcag ggcatatctc aattgataaa aggaagaaca agtatataga
      181 agatatatct aataggcaag gtgttgtaga gaatatttac tatcatatct ttttagaaat
      241 tatacatatc gctttaaaag agtttgagtc gtatcgtcaa ggaattatta gaacaattaa
      301 cgaataccag actgcactat tagaaaaagc aatttataat gaacaaagag agattactgc
      361 cgaggagatt gttcgagata ctattaaaaa tccagataga attactgagt taaccaagac
      421 tgattttgag aaaatccaag aaaagttgaa tgttcaaaga aaagttatag ataatctcgg
      481 agaagatcga aaaagaattg aagaggatta tcgttatgag gttcaattat tgaatgtttt
      541 atcgacttta caattaaaag taagctcatt aagtcacgag gttaaaaata acagaaattc
      601 tatcgcggca aatccaacta aaatacaaga agttttgaaa agaaagtatg actgggatgc
      661 actgaaactt gataaaccta gttctagaaa cttacctctg ctcattgaga cactctcgaa
      721 tgatttagaa aaagttttag atttagctga tacaatcatt gatgaaacta aaaaagataa
      781 ctttgttgtc aaagagttta aattagatga gataactggt ttgattatca ataagtggaa
      841 acaacaatat aattgggtga actttataat caaagtagaa ccagacgaaa aaatcacaat
      901 tt
//
LOCUS       DAVKZB010000220          882 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVKZB010000220 DAVKZB010000000
VERSION     DAVKZB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.220"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(263..433)
                     /locus_tag="VXA80_002039"
     CDS             complement(263..433)
                     /locus_tag="VXA80_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354465.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(446..880)
                     /locus_tag="VXA80_002040"
     CDS             complement(446..880)
                     /locus_tag="VXA80_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354466.1"
                     /translation="MLDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMG
                     SNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAHR
                     LSTIKDADIIFVMKGGKIVESGNHKYLMTLGGEYYSLYTKRK"
BASE COUNT          281 a          174 c          113 g          314 t
ORIGIN      
        1 aactaggaag cgagccgcag gctgtacttg agtacggcaa ggcaaagctg acgtggtttg
       61 aagagatttt caaagagtat taaacaaaaa gaaaaaccgc tactcctaag aatagcgatt
      121 taatcatgtt tttaagctac tactgtagtt gctctattat ttaagtgtta tgaataactt
      181 caatatcttt aaagcttttt gttagattta taatttcaac tatagccatc tctactcctt
      241 agtttacagc aaaaaattat gcttaattct tattgttttt gtaaacaatc agcgaaccca
      301 atactattcc attcaacaag cctaaagaaa taccatattc aactggaaaa tctaaaaaaa
      361 ttgcaaacac tattccaatc atcaccccta gtgaggctat tgttaaaatt acatattcat
      421 tttctttctt cattttcttt acacctcatt tcctttttgt atataagctg tagtactctc
      481 caccaagagt cattaagtac ttatgatttc ctgactcaac aatcttacca cctttcatta
      541 caaaaataat atccgcatcc ttaatcgttg acaatctatg agctataatt atttgagtac
      601 aaccctgact ttgtatatac tttgttattc tttcctcatt aatagtgtct aatgcactag
      661 ttgcttcatc taaaattaca atactaggat tatttattaa tgcacgtgcc agtgctatcc
      721 gttgcctttg cccacctgaa atatttgacc ccatctctga gatgatagta ttaaatttca
      781 tcggcatagc catgatttca tcatagattt gaactgcttt acaaacttcc tctatctttt
      841 gtgaagtaac ttcgtgcttt aaagttatat tatcaagaat ac
//
LOCUS       DAVKZB010000221          881 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVKZB010000221 DAVKZB010000000
VERSION     DAVKZB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.221"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(8..81)
                     /locus_tag="VXA80_002041"
     tRNA            complement(8..81)
                     /locus_tag="VXA80_002041"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VXA80_002042"
     tRNA            complement(95..168)
                     /locus_tag="VXA80_002042"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VXA80_002043"
     tRNA            complement(174..247)
                     /locus_tag="VXA80_002043"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VXA80_002044"
     tRNA            complement(263..336)
                     /locus_tag="VXA80_002044"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VXA80_002045"
     tRNA            complement(346..431)
                     /locus_tag="VXA80_002045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VXA80_002046"
     tRNA            complement(439..510)
                     /locus_tag="VXA80_002046"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VXA80_002047"
     tRNA            complement(533..605)
                     /locus_tag="VXA80_002047"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VXA80_002048"
     tRNA            complement(624..705)
                     /locus_tag="VXA80_002048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VXA80_002049"
     tRNA            complement(711..783)
                     /locus_tag="VXA80_002049"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VXA80_002050"
     tRNA            complement(808..880)
                     /locus_tag="VXA80_002050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVKZB010000222          873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVKZB010000222 DAVKZB010000000
VERSION     DAVKZB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.222"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            25..870
                     /locus_tag="VXA80_002051"
     CDS             25..870
                     /locus_tag="VXA80_002051"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV0354467.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGPLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          220 a          153 c          189 g          311 t
ORIGIN      
        1 taaatataat ataaggagaa atatatgtat cttattgaaa ttttaaaatc tatcttcttc
       61 gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca cttgatttta
      121 gcagaggagt ttatccaata ccaaaatcaa aatgaagcct ttatgtccat gtttaatgtc
      181 gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa gctcaatcct
      241 tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg gttgaaggtc
      301 ttgattgcta ctttaccttt acttggtgtc tttaaatttg atgattggtt tgatacccac
      361 ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc cttcatctat
      421 ttggaaaagc gcaataaagc gtgtgctatc gagccaagtg taacagagtt ggacaagctt
      481 ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt accagggact
      541 agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg ttcagttgtg
      601 acagaattta ccttctatct tgggattccc gttatgtttg gagctagtgc cttaaagatt
      661 ttcaaatttg tgaaagccgg agaactcttg agctttgggc cattgttttt gctcttggtc
      721 gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac cagctatgtg
      781 aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag tgttttgcta
      841 ctttacagtt ttgtccgttt atttgtataa gaa
//
LOCUS       DAVKZB010000223          859 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVKZB010000223 DAVKZB010000000
VERSION     DAVKZB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.223"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(257..853)
                     /locus_tag="VXA80_002052"
     CDS             complement(257..853)
                     /locus_tag="VXA80_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354468.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
BASE COUNT          316 a          139 c           96 g          308 t
ORIGIN      
        1 tccaaagttc ctcttattct tatcaaaaaa taatgtaatc agaaagagag aaaatccata
       61 ttcaataaga gttgctataa atgtgaaaac aacagaaatc actgtcaata ttgtttctat
      121 tttaattgta aaaagattag atagttcaga taatttatca atttgttcat ctaaaattcc
      181 cgacttcatc agtaaatcaa taaacaaata taaaagaaaa agataaataa tcagattcat
      241 taatttgtat aaactattat ttgtttgaaa tttcataaac ccgtccttcc tctattaaaa
      301 tcctttgaga tttgctaatc aattcttgat tcaataaatt atgagataca tatataatag
      361 tagagctttc tttcatggaa attaagcgtt tagcaatcat atcaatactt cttgcatcca
      421 ctgttgataa agtctcatca ataaaaatat aatcccaatt tttagcaaaa attaaggata
      481 agcctaattt gaattttgtt ccagtagatg ctgtcatata gttatctccg attttattat
      541 caaaaaaagt atttatttgt tctttatcct caaatgatag atggatttga aaagaatcac
      601 acaacaactg caaattttct tctatagtaa atggaacaac cccaagctga ttggtatcaa
      661 ggtatagtac atctttggag acacaattct ttaaaccact atcaaaagtc tctagcccag
      721 ctaaaagttg tagaagggtg ctctttcctg aaccattctc tccttcaact ctgtaaattc
      781 ccttatcaat ctctagtgat atatttttca atatacattt atcataactt ttagattcat
      841 tttcaatttt cataattta
//
LOCUS       DAVKZB010000224          847 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVKZB010000224 DAVKZB010000000
VERSION     DAVKZB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.224"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..268
                     /locus_tag="VXA80_002053"
     CDS             <1..268
                     /locus_tag="VXA80_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0354469.1"
                     /translation="LSITVELNYTYGILNLVYFDNGKGLDAKYKDKPEKILNVHETTR
                     EDGHGLGMWIVSNTIYKLGGEIKINPNYQGFFLSASILINESED"
     gene            271..>847
                     /locus_tag="VXA80_002054"
     CDS             271..>847
                     /locus_tag="VXA80_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354470.1"
                     /translation="MTSDIKLTYIDDKLDPLLVDYLYTISEKEHIFEYDEYKFDSSKD
                     SYQSLLENTSIASSDIIIVDSKLFENEFADSKSKFTGQELKIIYAIANPFIKIIVITQ
                     NNDLSKYGVIKKFATSRKCSGREQEEANRYYNDVLRKEIETLIKNVKEVRNVGQLLSE
                     NILSYEDSLIVEKANNLISKIPSYKDLTDEKI"
BASE COUNT          345 a           91 c          146 g          265 t
ORIGIN      
        1 actatcaata actgtagaat taaattatac ctatggaatt cttaacttag tatattttga
       61 caatggtaaa ggattagatg ctaagtataa agacaaaccg gagaaaattt taaatgtaca
      121 tgagactaca agagaagatg ggcatgggct tggaatgtgg attgtgagta atactatata
      181 taaattagga ggcgaaatta aaataaatcc taattatcaa ggatttttcc tttccgcttc
      241 aatattaatt aatgaaagtg aggactaaca atgactagtg atataaaact aacatatatc
      301 gacgataagc ttgatcctct tctagttgac tatttataca caataagtga gaaagagcat
      361 atttttgaat atgatgagta taaatttgat agtagtaagg atagctatca aagtttacta
      421 gaaaatacta gtatagcttc ttcagatatc attattgtcg attcaaagct ttttgaaaat
      481 gaattcgcag attcaaaatc taaatttact gggcaggaac tgaagattat atatgctata
      541 gcgaatcctt ttattaaaat cattgttatt acacaaaata atgatctttc aaagtatgga
      601 gtaattaaaa aatttgcgac atctaggaag tgttcaggaa gagaacaaga ggaagcgaat
      661 agatattata atgatgttct taggaaagaa atagaaacat taataaaaaa tgtaaaagaa
      721 gtaagaaacg ttggtcaatt attatctgaa aatattttat catatgaaga tagcctaatt
      781 gttgagaagg caaataattt aatcagtaaa attcctagtt ataaagattt aacagacgaa
      841 aagatta
//
LOCUS       DAVKZB010000225          841 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVKZB010000225 DAVKZB010000000
VERSION     DAVKZB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.225"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(1..841)
                     /locus_tag="VXA80_002055"
                     /pseudo
     CDS             complement(1..841)
                     /locus_tag="VXA80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          266 a          146 c          183 g          246 t
ORIGIN      
        1 ctttagaaag gttttaaaga cagtctgaaa aagaggatga acctgcttta gattgtcctc
       61 aataagcccg aaaaatttct ctggttcctt attctggaag tgaaacagca agagttgata
      121 gagctgatag tgatgtttca agtcttgtga atagctcaaa agcttgtcaa gaatttcttt
      181 atttgttaag tgcatgcgaa aagtagggcg ataaaaacgt ttatcgctca atttacggct
      241 atcctgttga atgagtttcc agtagcgctt gatagccttg tattcatggg attttcgatg
      301 aaactgattc atgatttgga cacgcacacg actcatagca cggctaagat gttgtacaat
      361 gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa acagcctggg agactgtttc
      421 agcctgagcc tagaaatttg aaagcgaagc tgtttagcca agtcatagta agagctaaac
      481 atatccatag taatgatttt gacgcgacat cggacggctc tatcatattt aagaaagtga
      541 tctcggatga cagcttgtgt tctaccctca agaacagtga tgatatcgag cttttcaaaa
      601 tcttgcgcaa tgaagctcat ctccatctcc cgattgaaac agtcactacc cggactgttt
      661 caacgtccca agacataatc tcaggaagac aagaaaaatc atgtttaaag tgaaaatcat
      721 tgagcttacg aataacagtt gaagttgaga tgaaaagctg atgggcaata tcagtcatag
      781 aaatcttttc aattaacttt tgagcaatct tttggttgat gatacgaggg atttggtgat
      841 t
//
LOCUS       DAVKZB010000226          804 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVKZB010000226 DAVKZB010000000
VERSION     DAVKZB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.226"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..>804)
                     /locus_tag="VXA80_002056"
     CDS             complement(<1..>804)
                     /locus_tag="VXA80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0354471.1"
                     /translation="TSLSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVL
                     YAALSTIVSVAVCSMYAYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLL
                     NTAWALVLPGAVSTYYIILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIF
                     MIIALYCIVGSWNSFTPPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQ
                     QIAAQGGGIGMRIAIKMGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
BASE COUNT          311 a          147 c          116 g          230 t
ORIGIN      
        1 aacctttcac agaacctagc aggattccct taacaaaatg tttctgaata aatggataaa
       61 tcatcataat tggaataaca ctaacaacaa tagtacccat cttaatagca atacgcatac
      121 caatacctcc accttgagca gcaatttgtt ggtaatatgt attacctgca gaaagtgata
      181 attcagcagt atcagttgta tttgtaacaa ctaaatttct caaataaata gataaaggca
      241 tcttagtgtc gtcattcaaa taaagtaatg gaggagtaaa actattccat gaaccaacta
      301 tgcaataaag tgctataatc atgaaaataa cttttgataa tggtaatact aaattccaaa
      361 aaattctaaa ttcacttgcc ccatcaattc tagcagactc acgaagctcc attgggattg
      421 atttaaactg tgttctaaac aaaattatat aatatgtaga aacagcacca ggtaaaacaa
      481 gagcccaagc agtattcaat aaaccgaggt ttgcaacttg tatataaagc gggatcattc
      541 ctccactaaa gaacattgga atcaaaataa gagtattcca gaatttttta cccctaattt
      601 caggaattgt aagtggatag gcatacatac tacataccgc aacactaaca attgtagata
      661 gtgcagcata caatacacta ttaccatatg cttgccatag tttagaatcc ccaaaaataa
      721 acttatatgc agtaatatct atttttaagg gaaataattt aacaacgttt ttagatagcc
      781 aaactggttc tgaaagtgac gtca
//
LOCUS       DAVKZB010000227          773 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVKZB010000227 DAVKZB010000000
VERSION     DAVKZB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.227"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            7..730
                     /locus_tag="VXA80_002057"
                     /pseudo
     CDS             7..730
                     /locus_tag="VXA80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          266 a          135 c          159 g          213 t
ORIGIN      
        1 gaagagatgt tagctgtatt aaaaacagct tatcaactta aacacgcaaa aggtggacga
       61 aaacctaaat taagcctaga agaccttctt atggccactc ttcactatgt gcgagaatat
      121 cgaacttatg aacaaattgc ggctgatttt ggtatccacg aaagcaactt aatccgtcgg
      181 agctaatggg ttgaagtaac tcttgttcaa agtggtttta cgatttcaag aactcctctc
      241 agttttgagg acacggtgat tgtggatgca acagaggtaa aaataaatcg tcctaaaaaa
      301 agaattagcg aatgattctg gtaaaaagaa atgccacgct atgaaggctc aagcgattgt
      361 cacaagtcaa gggagaattg tttctttgga tatcgctgtg aactattgtc atgatatgaa
      421 gttgttcaaa atgagtcgca gaaatatcgg acaagctggt aaaatcttgt ctgacagtgg
      481 ttatcaaggg cccatgaaga tatatcctca agcacaaact ccacgtaaat ccagtaaact
      541 caagccgcta atagctgaag ataaggctta taaccatgcg ctatccaagg agagaagcaa
      601 ggttgagaac atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa
      661 tcatcgtaaa cgcttcgaat tacgaatgaa tttgattgct ggtattatca atcatgaact
      721 aggattctag ttttgcagga agtctaatgt aatttttgca attcaagacg ttt
//
LOCUS       DAVKZB010000228          763 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVKZB010000228 DAVKZB010000000
VERSION     DAVKZB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.228"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            149..586
                     /locus_tag="VXA80_002058"
     CDS             149..586
                     /locus_tag="VXA80_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354472.1"
                     /translation="MENNFGKVSFNSPSLKNLLIGTSNVEHTPTSAFVIECRDVPKRN
                     EKNEVIDGTISKKVLLALQSEIVKAIQEVGGDLSSIKPFTIEIYNDESFLKKINNELI
                     IAKTIDLEGALLALKWVSDGRNGGAYREFKLIISEIKLSGAKS"
BASE COUNT          278 a          106 c          135 g          244 t
ORIGIN      
        1 ccacttcgcg tggggatttt tttgttgtca aaaaattttt atccccacat ttcaactaat
       61 taaaaatgag acaatatgac tgtcaaggag gtcatgaact gcttgatgaa attaatctag
      121 tgtgcacaca aagataaaga ggtaacaaat ggaaaataat tttggaaaag tgagttttaa
      181 ctcaccaagc ttgaaaaatc tactaattgg tacgtccaat gtagaacata ctcctactag
      241 tgcttttgtt attgaatgta gagatgttcc taaacgtaat gaaaaaaatg aagtcattga
      301 tggaacaata agtaaaaaag ttttattggc tcttcagtca gaaattgtta aggctattca
      361 agaggttggt ggagatttaa gtagcataaa acctttcact attgagatat acaacgatga
      421 atctttttta aaaaaaatta ataatgagtt aatcattgct aaaacaatag atttagaagg
      481 agcattatta gcgttaaaat gggtatctga tggtcgaaat ggtggtgcgt accgagaatt
      541 caagctcatt atttcagaaa taaagttatc gggtgctaaa tcatgatagt atctaaaata
      601 aaaaggaaac ttagtaaata tgattgtcgt gaatataccc ctacttactt atctgtttca
      661 gaaactatag aaagccgtaa agaaaggcag tactctctta ttaagaaact gttaaaggtg
      721 ttcctaatat ctgttatcat tacctttctt tcatcacatt ttc
//
LOCUS       DAVKZB010000229          722 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVKZB010000229 DAVKZB010000000
VERSION     DAVKZB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.230"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..623)
                     /locus_tag="VXA80_002059"
     CDS             complement(<1..623)
                     /locus_tag="VXA80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0354473.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLA"
BASE COUNT          222 a          142 c          115 g          243 t
ORIGIN      
        1 gcgagtaaat attttttatt agaattattt aaagcgatgc gttgagcctc tccctcttcc
       61 tcgctaaaag tagctagagc tccgttttga ataccattac agctaacgaa agctttagaa
      121 aattggagat tagagagatt ttgtagggtc aatgtgccaa caaaagcacc tgtaatatcg
      181 cgataatttc cacctatcaa aatcaaatct gttaattttc gttcgcttaa aatcagaaaa
      241 acaggtagac tgttggttac gacgcggata ttgtcaatag gcaactcacg cgcaaaaaac
      301 tctaatgttg ttcctggtcc aatgaaaata gtttctcttt cttctactag actgcctgca
      361 aaatgggcta tttcttgttt ttctgccgtt tggagggctt gtttttcaat atttgatcgc
      421 tcattagtca aaagggagtt ggttcgaagt ttttcagctc caccatgcac acgaatcagc
      481 aaatctttat cagctaattc ctgtaaatag cgccttgcag tcatatctga aacggctatt
      541 tcgtccataa tctgtttaac tgttatggtt cctttactat ttactatctc taaaattttg
      601 gttaattttt cttgtttgag catattatca cctcgtttcc tactactatc ttaccataaa
      661 ctagctcatc attcaaatac aaaaacaaca aaataaaaca aaaacaaaaa tatcgaaagt
      721 tt
//
LOCUS       DAVKZB010000230          715 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVKZB010000230 DAVKZB010000000
VERSION     DAVKZB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.231"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..>715)
                     /locus_tag="VXA80_002060"
     CDS             complement(<1..>715)
                     /locus_tag="VXA80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354474.1"
                     /translation="NRLKDSSIILISDRVIYIFLLFLFIIFKWHEYKVMIWADVLGRT
                     FSLLLSFWICKDIVFQSLSEFILDLRESFDNIRVGINLMLSNIASSMIIGIVRMGIQW
                     NWNIETFGKVSLTLSISNLLMTFINAIGLVVFPLLKRTKTENLSKIYSNLRNVLMLIM
                     FAILLFYYPLKIILDLWLPAYRDALIFMALIFPMSIYEGKMALVINTYLKALRMERDI
                     LKINTLIMLFSMLVTLITTL"
BASE COUNT          285 a          127 c           92 g          211 t
ORIGIN      
        1 tagagtagtt attagggtaa ctaacatact gaacaacata atcaaagtat ttattttgag
       61 aatatctctt tccatcctta gcgcctttaa atatgtatta atcaccaagg ccatcttccc
      121 ttcataaatt gacataggaa aaataagagc cataaaaatc aacgcatccc gataagctgg
      181 caaccaaaga tctagaataa tttttaaagg ataataaaag agcaatattg caaacatgat
      241 cagcatcaaa acatttctta agttggaata aattttagat aaattttccg tttttgtccg
      301 ttttaacaaa ggaaagacaa ctaaaccaat cgcattaata aaagtcatta ataaattaga
      361 gatgcttagc gtcagtgata ctttcccgaa tgtttcgata ttccaattcc attgaattcc
      421 cattcgaaca ataccaataa tcatactact tgcaatattg gataacatta agttgattcc
      481 aacacggata ttgtcaaaag actctctcag atccaatatg aactcggata aggattgaaa
      541 aacaatatct ttacaaatcc aaaaagaaag taggagagaa aatgtccttc ctaaaacatc
      601 cgcccaaatc attaccttgt attcatgcca tttaaatata ataaacagaa ataaaagaaa
      661 aatatatata acgcgatcac ttatcagaat tatagagcta tcctttaatc gattt
//
LOCUS       DAVKZB010000231          711 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVKZB010000231 DAVKZB010000000
VERSION     DAVKZB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.232"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..602)
                     /locus_tag="VXA80_002061"
     CDS             complement(<1..602)
                     /locus_tag="VXA80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV0354475.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEK"
BASE COUNT          243 a          138 c           84 g          246 t
ORIGIN      
        1 tgtttttcat caagttcctc ttgatacatt tttaattctt tagtttttcc catataacta
       61 accatactac tatcacttac atatgggaag tcctcataat atattacttt ataacgcata
      121 aattcaagcg cccttccaat actattcaca aaaacatgat caacatggtc accaagtgaa
      181 agcggacaat atacgacaca tttgtcgtct aaatgcatta acagctcttt tatgatatca
      241 ttctttaatg tgtcctcatt ttttaattca ctatagatat gacggtatag aaaattgcca
      301 tttctatctt tcctatagag acattcatag tacgataagt gtctaaaatc acactgtaga
      361 cgttcacaag ctaacctgtc ttctttcttc ctttcttcaa tcggatattt cccaaggtta
      421 cacaacttat gaaattgctt agcagagggc tgtagctgtt ggctcaaagg gtaaccagaa
      481 aatatagtaa taacaagtac aatttctcct tctgaagtta attttgaaat ataatcacca
      541 caggaaaaaa ttgcgtcatc taaatgtgga gataaaaata tatacttagt attgttactc
      601 ataaccattc cctctacaat ttatctaaaa attcactaag tgtctgatta aattccacat
      661 catcaaaaaa attcacctta ttcttaataa tgaatatttc gttaaataaa c
//
LOCUS       DAVKZB010000232          707 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVKZB010000232 DAVKZB010000000
VERSION     DAVKZB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.233"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>707
                     /locus_tag="VXA80_002062"
     CDS             <1..>707
                     /locus_tag="VXA80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0354476.1"
                     /translation="TYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNL
                     SYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKL
                     SKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHP
                     MSLSGGQKQRLAIASVMCKNSLFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIV
                     SHDIEFLNEVADEIFEL"
BASE COUNT          280 a           76 c          131 g          220 t
ORIGIN      
        1 acttatacta gaagtgaatt tttaaagcta gataaaaatg aattaaatgc tttaagttta
       61 agagataaag aattaagtaa attaaaagtt ccttatttaa aagaaggtgg agagtatcag
      121 ataaaaaatc ttagctacaa atttactgat gatgagtgtt taagcttaaa agatatttcg
      181 ttcaagcttg ggaaaattta tggcataata ggatccaacg gacgaggaaa atcaacgctt
      241 ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag aaatttattt taagggagag
      301 aagctatcta aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga tgtaaatcat
      361 caattattca cagatgaagt attcaacgag cttagattag gagtaaagaa ttttgatgaa
      421 gaaaaggcga aaatcatctt aaaagattta ggcctggacg aatttattga aaggcatccg
      481 atgagtttat caggagggca aaagcaaagg cttgcaatag catctgttat gtgcaagaat
      541 tctctatttg tcttttttga cgaaccttca agtggtatgg attattccaa tatgataaaa
      601 atctctgaac tgattaataa gtataaaacc atggataaaa taatttttat tgtttcccat
      661 gatatagaat ttttaaatga agtggcagat gaaatttttg aattgta
//
LOCUS       DAVKZB010000233          701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVKZB010000233 DAVKZB010000000
VERSION     DAVKZB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.234"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            293..592
                     /locus_tag="VXA80_002063"
     CDS             293..592
                     /locus_tag="VXA80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV0354477.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          262 a          130 c          120 g          189 t
ORIGIN      
        1 aatagataga tgtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc
       61 atgaaactag aatagagccc tcttagcaaa aatcattatt ttaatttatt tctaatcact
      121 ccttgacata aataactctc accaataaaa gactatgtct taaaaaaatg gtataataaa
      181 atcaatactt gggcttgatg gctatgctac taataacaat taggagagaa aatcaggcac
      241 ttgttaacaa caaggattat ccccttgaga tgaaaggaac tttagaaatc ttatgatgaa
      301 catgcaaaac atgatgcgtc aagcacaaaa acttcaaaaa caaatggaac aaagccaagc
      361 tgaacttgct gctatgcaat ttgttggcaa atctgctcaa gatcttgtcc aagcgacctt
      421 aactggcgat aagaaagttg tcagcattga tttcaatcca gctgtcgttg acccagagga
      481 ccttgagact ctttctgata tgaccgttca agccatcaac tctgctcttg aacaaatcga
      541 tgaaactacc aagaaaaaac tgggtgcttt cgctgggaaa ttacctttct aaaaacaagg
      601 agctagaaca atgcttgtcg ataacaaagg ctaagaaagg tgcaaaaatg actctataat
      661 atttgtagtg ggtaaatcca ctatagatat tatggagcct a
//
LOCUS       DAVKZB010000234          692 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVKZB010000234 DAVKZB010000000
VERSION     DAVKZB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.235"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..189
                     /locus_tag="VXA80_002064"
     CDS             <1..189
                     /locus_tag="VXA80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV0354478.1"
                     /translation="DYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMK
                     RIISKVDLLFQKVSQLFE"
     gene            complement(137..>692)
                     /locus_tag="VXA80_002065"
     CDS             complement(137..>692)
                     /locus_tag="VXA80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0354479.1"
                     /translation="NIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIK
                     PLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGG
                     FIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLA
                     PFEEQELITQKVEKLFEKVNQLWK"
BASE COUNT          242 a          106 c          100 g          244 t
ORIGIN      
        1 gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa agatgctatc
       61 aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat ttctaatcat
      121 gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt ttctcaactt
      181 tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc tcgcttagtg
      241 tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct ttcaattgtt
      301 tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc tctgaacttt
      361 cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag tctttatcga
      421 ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt agctgattat
      481 gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa tcgttatcca
      541 acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca cctttattat
      601 taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt cattgaaaaa atatctttta
      661 tttttataac aacccaattc ataggtatat tc
//
LOCUS       DAVKZB010000235          657 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVKZB010000235 DAVKZB010000000
VERSION     DAVKZB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.236"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>657
                     /locus_tag="VXA80_002066"
     CDS             <1..>657
                     /locus_tag="VXA80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV0354480.1"
                     /translation="TDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSNK
                     LKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTAN
                     IKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIG
                     NFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKPK
                     "
BASE COUNT          239 a           83 c          119 g          216 t
ORIGIN      
        1 gacagattat gatgagaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagattctg agggtaaaac ctaatggaaa gattatttta aagtcaacag ttccgatcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc tttttttaat gaactagatt cttttgctga
      421 gaggagaagt ttaaatgctg aagttgtaat aaaaggtgtt tgtttagatc caagaattgg
      481 aaatttttat aataatcctt cttttggatt tggaggatat tgtcttccca aagatactaa
      541 acaattaaaa aaagaattta tagaaataaa tgccccagtg atagaagcga ttgatatttc
      601 aaacacaaat agaaaacagt tcatagttaa acaaatattg gaacgaaagc caaagat
//
LOCUS       DAVKZB010000236          650 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVKZB010000236 DAVKZB010000000
VERSION     DAVKZB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.237"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            261..>650
                     /locus_tag="VXA80_002067"
     CDS             261..>650
                     /locus_tag="VXA80_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354481.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVDLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQ"
BASE COUNT          233 a           91 c          149 g          177 t
ORIGIN      
        1 ttgaggcagt atcactgctt gtacaagctg aggcatcagc gaagtagaag taaatgatct
       61 cccatacgat aggactcgta gaatgaggag ggaaaccgac gaaatgataa ggtaagtccg
      121 aaccgctgag tgcgtggctc tgctcttccg atctgagaca gtcgaaaagt agaaggggat
      181 ggtattggta tcaatcaatg agtaacaaag tatcagcatg attcgaaaca tttacttgta
      241 atttgcggag gaacatcaat atggatggaa taacaaaaga ttttataaaa acggctaaac
      301 taatgaaaca attatggccc caattgaccg ataaagaagc tattgatgaa gtaaaaaaat
      361 atacgaatgg caaaaatact gcaatcttta ctgaagttga aggtgacaca attgtagatc
      421 tagcactatg ttcactcaga tttgattatg ttgaaggttg taaatatagt cctgttggat
      481 tcttagaagg gattattgtt gacgaggaat atcgtttaaa ggatattgct aaaaatctct
      541 gtacaaaatg tgaggaatgg gcgaaaaata aaggatgtaa ggaatttgca agtgactgta
      601 ctttaacgaa tacggattct ataagatttc atctcaatat tggatttcag
//
LOCUS       DAVKZB010000237          644 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVKZB010000237 DAVKZB010000000
VERSION     DAVKZB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.238"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            81..230
                     /locus_tag="VXA80_002068"
     CDS             81..230
                     /locus_tag="VXA80_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0354482.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVL"
     gene            273..638
                     /locus_tag="VXA80_002069"
     CDS             273..638
                     /locus_tag="VXA80_002069"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0354483.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
BASE COUNT          269 a           79 c          113 g          183 t
ORIGIN      
        1 attagaaaga aaaaatcgaa aaattagctg agaaatactc tgataactta aacatcaaag
       61 ttcaagagag agttcgtgaa atggcaaatg ataataagag ccattatttg atatacagag
      121 ttttaggtat ttcatttgaa gaaggagaaa atatcgattt gtatcaaaat aaaggtcgtt
      181 ttttatacaa atatgctggt tcatttttag aagaagctgc agtactatga tttaacgaaa
      241 aatttggtat agaaaatact taaaaagtta acattcctaa ttctgaaagt acaaaaccta
      301 agacttttga aattgattgt ttagtcggag aaaaacacgc atacgaaata aaatggtggg
      361 atgcaactac agatggagac catataacta aagaacacac tagaataaaa gttattcata
      421 acaaaggata tataccaatt cggttaatgt tctactatcc aaatagaact caagccataa
      481 aaattcagca aactttagaa acattgtata acggtattgg agggaaatat tattatggag
      541 attctgcctg ggaacattta agagcagtga ccggtattga tttacttagt attctaacag
      601 atattgcaaa taaaaaaaca ggggtaaaat caaaatgaca gtat
//
LOCUS       DAVKZB010000238          615 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVKZB010000238 DAVKZB010000000
VERSION     DAVKZB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.239"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..572)
                     /locus_tag="VXA80_002070"
     CDS             complement(<1..572)
                     /locus_tag="VXA80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354484.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTA
                     WIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSY
                     LLQNQSEVDLDIFYKVLPLLYKEKYTMGFN"
BASE COUNT          222 a           95 c           90 g          208 t
ORIGIN      
        1 taattgaatc ccatagtata tttttcttta taaagcaaag gtaaaacttt ataaaaaata
       61 tctaagtcaa cctcactctg attctgaagc aagtaggagt caagcaaatt ttcaaaaata
      121 gctatcaata tgattggtgt aaggtttttg tattcacatg tcaagtattc tgagtatcgt
      181 gcagatgata tttcttttat ataacttttt gaaagcaaga ttttttcaaa ttcatcatct
      241 attattaatc ttctatttcc aatccaagcg gtattgctac ccaaataaga ttgtacaaat
      301 agcaatatgt aagaaaacat gaacgtaaat actgaaagag gtagaattct atgcgtaaaa
      361 aaatagacga taatggtagc caggagtaaa gctatctctc gtgacaatcg aattcttttc
      421 agcatctttt ttaacagtcc gtaaggttgt ccttggcaat attcttgaac taaattaatc
      481 ggaatgacat ctctcaccat ttctggaaag tagagtaagt ttataggttg aaatctccag
      541 ctcccatcat aagtaaatgg atatagaacc aaatacttaa aagatttttt tcttataaac
      601 ttaattatca caata
//
LOCUS       DAVKZB010000239          613 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVKZB010000239 DAVKZB010000000
VERSION     DAVKZB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.240"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            8..262
                     /gene="blpM"
                     /locus_tag="VXA80_002071"
     CDS             8..262
                     /gene="blpM"
                     /locus_tag="VXA80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV0354485.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            278..481
                     /gene="blpN"
                     /locus_tag="VXA80_002072"
     CDS             278..481
                     /gene="blpN"
                     /locus_tag="VXA80_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV0354486.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          184 a           77 c          162 g          190 t
ORIGIN      
        1 atttgtcatg gatacaaaaa ttatggaaca atttcatgaa atggatataa ctatgttatc
       61 tagtattgaa ggaggcaaga ataattggca aactaatgtc ttagaaggtg gtggtgctgc
      121 ttttggtggc tgggggctag ggacagctat ttgtgctgcg agtggtgttg gagcaccatt
      181 tatgggagca tgtggataca taggagctaa atttggtgtg gatctttggg caggagtaac
      241 aggggcaacg ggtggatttt aataaaagga gacttgtatg aatacttatt gtaatataaa
      301 tgaaacaatg ctgagtgagg tttatggagg taattccgga ggagcagctg tagttgctgc
      361 tttaggttgt gcagcgggtg gagtgaaata tgggagactt ctaggaccat ggggcgctgc
      421 aataggagga attggaggag cagtggtttg tggatattta gcctataccg ctacatcata
      481 aaaatgaaag ctaaatttta tgaaggctat attctttatt attctcttcg cttttcaaac
      541 ctatcttatt tatctatcca taagtatctc cgataaaaaa caaaagacta ttgaattaac
      601 atcattgaat tgt
//
LOCUS       DAVKZB010000240          606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVKZB010000240 DAVKZB010000000
VERSION     DAVKZB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.241"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
BASE COUNT          228 a           84 c          116 g          178 t
ORIGIN      
        1 aaggcactat taaagacttt aagtctaaaa aggtatcaaa gtatgccaga gataaaaaaa
       61 tataaatatg acactttaaa ggcgattaga aaattaaaga aatgctagaa gacttttaga
      121 tgacagttac caagagggtc taattttcac tgctacttat gcagtgggaa ttggaccttt
      181 ttatataagc aaattctatt tcaaaattgg ttaccaagaa agacttcttt tcactgttaa
      241 gtataagagg gtaatttata gaattttgtt acatcaagat aaatattttc tgatggtcaa
      301 attttaaaaa gattaataac tctcgataaa ttttgaacct taacaattga atagaccaag
      361 acaagacgtt aattattttt ggagcgtaat aacctatccg tcaagatctt tcggctgaat
      421 aattcggcaa aaaaagttct gctaagcaaa aagaaagtga gcgataagta agagtttgaa
      481 tatagggagg gataccctat ccgctatgaa gaaaatgagg ataatgatac ttctaaggtt
      541 gaagccggct catccggaat agaggcggag gtgagattcc tatgttgcta atccaaagca
      601 cttgtc
//
LOCUS       DAVKZB010000241          602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVKZB010000241 DAVKZB010000000
VERSION     DAVKZB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.242"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            293..559
                     /locus_tag="VXA80_002073"
     CDS             293..559
                     /locus_tag="VXA80_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV0354487.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGEILPKQVLEEQQQEVFN"
BASE COUNT          216 a           76 c          131 g          179 t
ORIGIN      
        1 taggaataaa aaacttaact ataaactatt atttactaat attttttgct ttctgcaagt
       61 taataaatag tatagtaaaa ctttttattg caaggagaat aacgaaatta agatatttta
      121 gatactcaag attattgaaa tcttagacta aaaaataggt actgctacct ttaagaagat
      181 agtagacgta tatacttttt taagaaaatc aaaaagatac tataaaaaat ctatttgttt
      241 attgaattta agactttggt aacaaattga aaataaaaag gaggtattca tcatgaatac
      301 aaaaatgttg tcacagttag aggttatgga tactgaaatg cttgcgaaag ttgaaggggg
      361 gtatagctcc actgattgtc aaaatgcact gattacagga gtcactacgg gaattataac
      421 tggtggaact ggagcaggtt tggccacttt aggagtagct ggacttgctg gtgcatttgt
      481 tggagcacac attggtgcaa ttggggagat tttgccaaag caggtgttgg aggagcagca
      541 gcaagaggtc ttcaactagg cattaaaaca ggaacatggc aaggtgcagc aactggtgct
      601 gc
//
LOCUS       DAVKZB010000242          602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVKZB010000242 DAVKZB010000000
VERSION     DAVKZB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.243"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..>602)
                     /locus_tag="VXA80_002074"
     CDS             complement(<1..>602)
                     /locus_tag="VXA80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEV0354488.1"
                     /translation="VALNYNESKLLHLNIANIHIITLYSFLSDKNGTMIDIFTETPWC
                     REGETEDEDYLDISIFEVFEPNDWLKIDNCKIDSVIASYQRLVDNKLKYEGADRTILK
                     IVIAADMAEDKTKRELLLNWAQCLSDWNLKYSKNCEMAIINDLQIKSRVRKLNSKETE
                     TLTNILVNSNDNYELCFGSSVLLKSKPQADLFWNKLDNET"
BASE COUNT          189 a          104 c           79 g          230 t
ORIGIN      
        1 tttgtctcat tatccagttt attccaaaac aaatcagctt gtggtttcga ttttaatagc
       61 actgatgaac cgaaacaaag ttcatagtta tcattactat ttactaaaat attagttagt
      121 gtctctgttt ctttgctgtt taatttacga actctagatt ttatttgaag gtcatttatt
      181 atagccatct cacaattttt tgagtatttt aagttccagt cagaaagaca ttgcgcccaa
      241 ttaagtaaca actctctttt agtcttatct tctgccatgt ctgcagcgat aactattttt
      301 aaaatagtac ggtcagcgcc ttcatatttc agcttattat ctaccaatct ttgatacgag
      361 gcaataactg aatcaatttt acaattatca atttttagcc aatcgtttgg ttcaaatact
      421 tcaaaaattg aaatgtctaa ataatcttca tcttcagttt ctccttcccg gcaccaagga
      481 gtttcagtaa aaatgtctat catagttcca tttttatctg acaaaaaact atataaagtt
      541 ataatgtgga tgttagcaat atttaggtga agtaatttcg attcattata atttaaagca
      601 ac
//
LOCUS       DAVKZB010000243          550 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVKZB010000243 DAVKZB010000000
VERSION     DAVKZB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.244"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>550
                     /locus_tag="VXA80_002075"
     CDS             <1..>550
                     /locus_tag="VXA80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEV0354489.1"
                     /translation="TNSSINAFCIGGEKGINEIPEVTKIAKFYGINLLVDTVMSQDLE
                     YFPDIVWRLEGSLFECGVILQYYLGRLLFQKGNTSILCGESADEIMTFKYHSVNYNQF
                     CNDKQKSVYFSYSDYPFYVTNSIVLKKNSLLLHSFGIHPRYPYKMSEIVEMSKKISDL
                     NDKKEFHKKNCELRFQDSVLDNI"
BASE COUNT          198 a           66 c          100 g          186 t
ORIGIN      
        1 acgaattctt caattaatgc attttgcata ggaggagaaa aaggaattaa tgagattcct
       61 gaagtaacta aaattgcaaa attttatggt ataaatttat tagttgatac tgtaatgagc
      121 caagatttag aatattttcc cgatatagta tggagattgg aaggaagtct ctttgaatgt
      181 ggtgtcatac tgcagtacta tttaggcagg ttgttgttcc aaaaagggaa cacaagtatt
      241 ctttgtggcg agtcagcgga tgaaataatg acttttaagt atcattctgt gaactacaat
      301 caattttgta atgataaaca gaagtcagta tattttagtt attccgacta tccattttat
      361 gttacaaatt caattgtttt aaagaaaaat agcctacttt tgcatagttt tggcattcat
      421 cctagatatc catataaaat gtctgagatt gttgaaatgt ctaaaaaaat atcagatcta
      481 aacgataaaa aagaatttca caaaaagaac tgtgagttga ggtttcaaga tagtgttctt
      541 gataacataa
//
LOCUS       DAVKZB010000244          535 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVKZB010000244 DAVKZB010000000
VERSION     DAVKZB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.245"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..228)
                     /locus_tag="VXA80_002076"
     CDS             complement(<1..228)
                     /locus_tag="VXA80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354490.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIY"
BASE COUNT          192 a           99 c           74 g          170 t
ORIGIN      
        1 ataaattggt cttaatttac aaaagattga ggaaactagt aatagcaaag tatgtaagca
       61 ttgagacaaa atatagaata acggtatcgc caatacaaag gttaagatgg catgagaggg
      121 ggttcctaag atgtccaatt ttataaatac tgggctgaca caaaatatcg tgcttataag
      181 cagtaaaatc attaaaattg tgtcaaaaaa ataacgctta tttttcattt agtctacttt
      241 ccattgttaa ataatactac tgctaacacc aagccaactt agcacattgc atcacctaat
      301 cctgcgctac ttgtccataa tcctttaatg tttctactaa aattattcca atttccccca
      361 tcaagatagc ttatttatcc tagacaatgc ctactgcaac agtataaata agattcatca
      421 gccatctttt ctggcaatca tatgggtagc ctaatgatat ttctagttac tcaaaaatat
      481 ctagctatac tagagttatt tctaatccct aggacacttg ttaatccaaa tataa
//
LOCUS       DAVKZB010000245          523 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVKZB010000245 DAVKZB010000000
VERSION     DAVKZB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.246"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(106..>523)
                     /locus_tag="VXA80_002077"
     CDS             complement(106..>523)
                     /locus_tag="VXA80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV0354491.1"
                     /translation="KNKADQDSKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAETLLEKVTDSSIRQNAVETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          130 a          107 c           83 g          203 t
ORIGIN      
        1 gtttactatt taaaattact tttgtcaagt ttctgttacg tttttctaaa aaagagaagt
       61 aacacgaaag gttacttctc cataaaatat gctccattca ttttactact gtataggagt
      121 cggttgactt tcttttaaca aagccaagag actatctact tctgctgaaa tagtgttatt
      181 atctttcgtt ccgagaagaa gactactttt tagaccagtc aatgtctcca cagcattttg
      241 tctaatacta gaatctgtta ctttttctag caaggtctcc gcctctgcta tcttagcgtt
      301 aataacagaa tgctctactt gaggaatttc agcctcatct gtagtatctt cagcttcttc
      361 ctctgtctct tccgtatcag tgcttggttt ataaagatta tctgctgaag gatttaaacc
      421 agcgtgattc tctttttcat cagattcagg gtgagttggc tcacttactt catcatgttc
      481 cttatcttcc tcaggtttac tgtcttggtc tgccttattt tta
//
LOCUS       DAVKZB010000246          523 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVKZB010000246 DAVKZB010000000
VERSION     DAVKZB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.247"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
BASE COUNT          204 a           88 c           58 g          173 t
ORIGIN      
        1 tccactgatt gctatcaata tttcaattgg taaaaaatag taattccaaa taaaatgaga
       61 aatcagcaat gatagaaaaa taacaatatc aattttcctg ttgttgacat actctgtaat
      121 tttatgatac agtaatgcaa caataactga aattaaaaca atcaacataa tactattatc
      181 ctatccttct cactaactct atttccaaaa tttaattttt gggggactta gtaatgaaat
      241 tacaattaac aacacaccta aaataaataa aaatatctcc caaactttat tctcagtttt
      301 aataagtgga ataagataca tactgactaa tatgcaactt gcccctataa tggcaaatat
      361 tattctagtt ttatctccta attgaaacat gtttgatccc tatatacact tcaagcaaaa
      421 gttggatttc tgaaatggct aaagataaag ataatggttg cgagctattt aaactatcat
      481 caatattctg ctcgattaaa acaaagtctg gtcttacctc taa
//
LOCUS       DAVKZB010000247          520 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVKZB010000247 DAVKZB010000000
VERSION     DAVKZB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.248"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            30..>520
                     /locus_tag="VXA80_002078"
     CDS             30..>520
                     /locus_tag="VXA80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0354492.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNL
                     LSF"
BASE COUNT          192 a           56 c          105 g          167 t
ORIGIN      
        1 aagatttagg tgcaactaat gccagaaaca taggtatcaa aaattctaga ggtaagtata
       61 tatcattttt agacgatgat gatgaatata tgccagatcg aattttaaag ttgatggctt
      121 gttttaaaaa gagtagaatg aagaatttag ctttagttta tagttatgga ataataattt
      181 atccaaatgg tacacgagaa gaggagaaga ccgattttgt tggaaatccc ttgtttgttc
      241 aaatggttca caatatagca ggtacgtcat tttggttgtg taaaaaagag gtgctagaat
      301 taattaatgg ttttgagaaa atagattcac atcaggacgg tgttgtttta ttaaaactac
      361 ttgctcaagg ataccaaatt gatatagtgc gagaattctt ggtgaattac tacgctcaca
      421 gtaaagaaaa cggtatcact ggagtgacac aaaaaacaat taatgcagat gaagaatatt
      481 ataattactg taggaaatat tttaatttat tgagtttcaa
//
LOCUS       DAVKZB010000248          476 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVKZB010000248 DAVKZB010000000
VERSION     DAVKZB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.249"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            26..>476
                     /locus_tag="VXA80_002079"
     CDS             26..>476
                     /locus_tag="VXA80_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000507009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354493.1"
                     /translation="MGFSPGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYD
                     KPLLLPLISFSIIIALVQKLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKL
                     QSKSELPCVALSYISLIKPYLSESVREQIDLLEVKRFKKINHPISLYG"
BASE COUNT          161 a           72 c           67 g          176 t
ORIGIN      
        1 ctatcgaatt ctctttcacc aactaatggg atttagtcca ggaatagcag tgttttataa
       61 ggagagtcaa acaactaaga atctgtttaa attttactac ttcttgtact ttacaacact
      121 aatttcatac tattttttct ttacttttgt gtatgacaaa ccgttgcttt tgcctcttat
      181 ttcattttct attatcatag cattagtaca aaaactttat agaattgaga atcaacaatt
      241 atttctgtta aaatcaaagg tactaacaat actggaatct aagaagaatt gtgagtttaa
      301 tcttcaggat tatcatgaaa tttggaaatt gcaatcaaaa tctgagttgc catgcgtggc
      361 tttatcttat atcagtttaa tcaagccata tctttcagag agtgtgcgtg aacaaattga
      421 tttattagaa gttaagcgat ttaaaaaaat aaatcatcca atatcacttt atggta
//
LOCUS       DAVKZB010000249          474 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVKZB010000249 DAVKZB010000000
VERSION     DAVKZB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.250"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(260..424)
                     /locus_tag="VXA80_002080"
     CDS             complement(260..424)
                     /locus_tag="VXA80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV0354494.1"
                     /translation="MESLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEWDTISF
                     DSDFMPFQQS"
BASE COUNT          174 a           79 c           65 g          156 t
ORIGIN      
        1 taaactaagt gattcaatcc tttttaagct atctaaacac attaaagtca tcaagcctta
       61 ataaactgaa agagcctggg taacaactca atttcagaag aaaaaaagta aatcttccca
      121 caataaaaag catagtatca ggtgtttcaa cacctgatac tatgcttttt tctatgattt
      181 tcattgagta taagcaacgt gattagataa gagttttaca gagtactaaa tataagattt
      241 aacttttttg agaaattggt tacgattgtt gaaacggcat aaaatctgaa tcaaaggaga
      301 tagtatccca ttcttcagga ttgataaaat ttaaaaatag atttttaaat tcttccagct
      361 catcatctga atgacaaatc cattctttac cagtcgagac ataccatttt ccaagacttt
      421 caattcttca ctcgtcaaac acggtatttg agagcatttt tcgaaattta taat
//
LOCUS       DAVKZB010000250          452 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVKZB010000250 DAVKZB010000000
VERSION     DAVKZB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.251"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            1..452
                     /locus_tag="VXA80_002081"
                     /pseudo
     CDS             1..452
                     /locus_tag="VXA80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          181 a           74 c           89 g          108 t
ORIGIN      
        1 agagcggaaa atgccatcct aaaaaagtta agagaactcc gattgaagga ggaaaaagag
       61 aaagaagaaa gacagaaatt gttcaagaat taatgactga gttttcgtta gatcttcttc
      121 taaaagccat taaactagct cgttcgacct actactatca cttgaaacag ctagataaac
      181 cagatacgga ccaagagctt aaagctgaaa ttcaatccat ttttatcgaa cacaagggaa
      241 attatgctta tcgtcgaatt tatttagaac taagaaatcg tgcttatctg gtaaatcata
      301 aaagagttca aggcttgatg aaagtactca atttacaagc taaaatgcga cagaaacgaa
      361 aataaaggag acgttggtaa gaaggcagag aatctcattc aaggccaatt tgaaggctct
      421 aaaacaatgg aacagtgcta cacagatgtg ac
//
LOCUS       DAVKZB010000251          450 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVKZB010000251 DAVKZB010000000
VERSION     DAVKZB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.252"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(33..>450)
                     /locus_tag="VXA80_002082"
     CDS             complement(33..>450)
                     /locus_tag="VXA80_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diadenosine tetraphosphate hydrolase"
                     /protein_id="HEV0354495.1"
                     /translation="FDTSFVEMSYSQFFEGYCILFSKEPYPSLSDMPMEFRKEFLMEM
                     SILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRKTVVHRYDSSVFSN
                     PEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
BASE COUNT          161 a           78 c           58 g          153 t
ORIGIN      
        1 caaggtatta aatctaatcg aaaatgtgga gttcagtttg aatcatctaa taattgttga
       61 tactctctat aatgtctatc aatagcagac ctcagttttt taataattgg tccatgcttt
      121 tctatagtaa attcaaactc aggattgcta aaaactgaag aatcatatct atgaactaca
      181 gtttttcttc tctcttcctc ttcccacatg tatctcggaa ataaatgagc atgaagtaca
      241 ggtgttgaat ttcctaagat atcataatta attctatagg catttaacac ttcaagcatc
      301 gcatcaccta atatactcat ttccattaaa aattcttttc taaattccat tggcatatca
      361 gataaagaag gatagggttc ttttgaaaaa agaatacaat atccttcaaa aaactgagaa
      421 taagacattt ctacaaagct agtatcaaat
//
LOCUS       DAVKZB010000252          428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVKZB010000252 DAVKZB010000000
VERSION     DAVKZB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.253"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(127..>428)
                     /locus_tag="VXA80_002083"
     CDS             complement(127..>428)
                     /locus_tag="VXA80_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354496.1"
                     /translation="IFSMLMLMLVIHLLGDIWTVKLMLVNNHKYVDHILFRTVKHPNY
                     FLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEEKLLHEVIIPNGSIKR"
BASE COUNT          156 a           79 c           53 g          140 t
ORIGIN      
        1 tactttggca aaaatattct caaccttgct tcataattca tagaactatt gtaccatatt
       61 ttgtttcgca tgaagtctat tactatatca cttcaatatg aactgtatat gaatcgaatt
      121 ttgtatttat ctctttatgc ttccatttgg gattataact tcatgtaata gcttttcctc
      181 ttcagctatt cgtcgataca aaataactgc ataaactgga aaaactaaaa caaaagtcat
      241 ataggcatga ctcaacaagg tcaagccaat caactcaggt aaaatattta aaaagtaatt
      301 agggtgtttt actgtcctaa acaagatatg atctacatat ttgtgattat tgacaagcat
      361 aagcttcact gtccaaatat ctcccaacaa gtgaatcacc aacatcaaca tcagcataga
      421 aaaaataa
//
LOCUS       DAVKZB010000253          422 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVKZB010000253 DAVKZB010000000
VERSION     DAVKZB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.254"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            <1..>422
                     /locus_tag="VXA80_002084"
     CDS             <1..>422
                     /locus_tag="VXA80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEV0354497.1"
                     /translation="RFFNELTKEKIYQNSTFRNYAKRSLTRATPFGLFSSVGVGSFSK
                     VSYPQQIRENYSKKVSVSGEWISSLCMMLENEDSVLLQLHLQWNQKVLELSDKYQLNN
                     INYWGVSEQSRDILIKKTALLEFIKKLTYKSEVSVLDL"
BASE COUNT          151 a           48 c           82 g          141 t
ORIGIN      
        1 acgatttttt aatgaattaa ctaaggaaaa aatatatcaa aattctacgt ttagaaatta
       61 tgcaaagagg tcgcttacaa gagcaactcc atttggattg tttagttcag ttggtgttgg
      121 ttctttttca aaagtttcat accctcaaca gattagagag aattatagta aaaaagtgtc
      181 tgttagtgga gagtggatta gttcgctttg tatgatgtta gaaaatgaag attcagtttt
      241 attgcaatta catttacagt ggaatcaaaa agtactggag ctatcagata agtatcaatt
      301 gaataatatc aactattggg gtgtttccga gcagagtaga gatatactta tcaagaaaac
      361 tgccctttta gaatttatta aaaaattgac atataaatcg gaagtaagtg tattagactt
      421 gg
//
LOCUS       DAVKZB010000254          407 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVKZB010000254 DAVKZB010000000
VERSION     DAVKZB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.255"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            94..390
                     /locus_tag="VXA80_002085"
     CDS             94..390
                     /locus_tag="VXA80_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV0354498.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          141 a           54 c           82 g          130 t
ORIGIN      
        1 atatagacga acatttgaag agagttagga atacaacatg tgcgaattaa ctctcaataa
       61 aacatacata atatacaaaa ggagtacact aaaatgaaat ctaaaagaat ggaatttcat
      121 aataaatttc ttattgtttc agcaatgtta gcagtcatta gttggttaag cttaggtgtt
      181 gtttcttttc ctatgttggc tgggacatta ggaatttcga caaaagctgc tgctactgta
      241 gtcaatttga ttagtgctta tagtacagtt acagcagtaa tttcaattgt aggtgcaatc
      301 acaggagtag gttctattgg ttcgggtatt gcagcaacag ttctttatat attgaaaaag
      361 aaaggtgctg ctaaagcagc tctatggtag aactagatga aaatact
//
LOCUS       DAVKZB010000255          405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVKZB010000255 DAVKZB010000000
VERSION     DAVKZB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.256"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            127..>405
                     /locus_tag="VXA80_002086"
     CDS             127..>405
                     /locus_tag="VXA80_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354499.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYF"
BASE COUNT          119 a           83 c           70 g          133 t
ORIGIN      
        1 ccttgcctta gcttagatcc tggatggttt cttttttcac ccaatgggtg ttttttacta
       61 gacaaaaaag agttccccct ttatggtata agtgtagaaa aaaacacaaa aagaaaggaa
      121 actcacatga acagtttacc aaatcatcac ttccaaaaca agtcttttta ccaactatct
      181 ttcgatggag gtcatttaac ccagtatggt ggtcttatct tttttcagga acttttttcc
      241 cagttgaaac taaaagagcg gatttctaag tatttagtaa cgaatgacca acgccgctac
      301 tgtcgttatt cggattcaga tatccttgtc cagttcctct ttcaactgtt aacaggctat
      361 ggaacggact atgcttgtaa agaattgtca gctgatgcct acttt
//
LOCUS       DAVKZB010000256          383 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVKZB010000256 DAVKZB010000000
VERSION     DAVKZB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.257"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
BASE COUNT          126 a           59 c           61 g          137 t
ORIGIN      
        1 gcttattatt ctagtgaaaa attgaatgag ccactattta tttaatatgt atgatgaggg
       61 aatgactcaa aaacttcttg tcgatataag aacatctgag atagtatttt aaaacgaaag
      121 gactataaaa gctcatttcc ttcgcactta ttttatagag atctatcatt atactcttta
      181 gaaatcaaaa agcaacacac cttgtcatcg ttttatcgta aatctgttaa tgagggggct
      241 agtcttatct gcaatctcaa atcagtgttt tgagctgaat ggcactcgtt ttctcgtttg
      301 ctctttgatt ttaattgggc cttagtaaat actactctta aattaaacta tagtgaattg
      361 aaataagatg tgaacaactc tat
//
LOCUS       DAVKZB010000257          369 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVKZB010000257 DAVKZB010000000
VERSION     DAVKZB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.258"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..>369)
                     /locus_tag="VXA80_002087"
     CDS             complement(<1..>369)
                     /locus_tag="VXA80_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354500.1"
                     /translation="SRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHF
                     TTYGKQEGVAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLE
                     RFNQLLFRMDSGFATPKLYD"
BASE COUNT          121 a           69 c           85 g           94 t
ORIGIN      
        1 aatcgtataa ttttggggtc gcaaagccac tatccattcg aaagagaagt tgattaaacc
       61 gttctaaaac aggtgtgata aagctgtctg cctcttcaga acaataacga ttaccaggac
      121 gaagctgggc attgaaacaa taacctgtct tcccctcgaa agcataaaga ggatgatagc
      181 catgagcacg atagtgggcg ttataagcaa caccttcttg cttgccataa gttgtgaaat
      241 gggtagaatc gatatctaca atgagttggt ttagctggtg aaactgtaaa aagaattcga
      301 ccaattcaag gttgaggcat cgcaaactat ggactgtttc ctcgtcagtt ctggaaagaa
      361 aacgggata
//
LOCUS       DAVKZB010000258          352 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVKZB010000258 DAVKZB010000000
VERSION     DAVKZB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.259"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            complement(<1..>352)
                     /locus_tag="VXA80_002088"
     CDS             complement(<1..>352)
                     /locus_tag="VXA80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0354501.1"
                     /translation="NTVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRV
                     CQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDS
                     SSLIKNEVRMMMSCI"
BASE COUNT           94 a           79 c           80 g           99 t
ORIGIN      
        1 gatacagctc atcatcatac gaacttcgtt tttgattaag cttgaactat ccgttttatc
       61 gccaaaaaat ccctccttca tctccttgat gaaattctcg gcttgaccac gtccacgata
      121 aagctgaaac tggtcttggc ttgttccact cgtcatattt gtaacgagag aaataacatc
      181 gtagaacaag tttccttctt ttcgttcaga gaactggcag acacgacgct tgtgcgacca
      241 agatcctgct tgatagagag tttctgagta ggcggagtgg ggcaagatgg ttaagtcctc
      301 atcctgtggg caagggaggg aaaggtctcc aagacggctc agaacagtat tt
//
LOCUS       DAVKZB010000259          351 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVKZB010000259 DAVKZB010000000
VERSION     DAVKZB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.denovo.260"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            63..230
                     /locus_tag="VXA80_002089"
     CDS             63..230
                     /locus_tag="VXA80_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0354502.1"
                     /translation="MSLIYSSAKKDFLYWNVLIFIMELPNDVKVQFYELRKKVQSFNQ
                     LSKRFGMDVSG"
BASE COUNT          137 a           41 c           61 g          112 t
ORIGIN      
        1 accaaatttt gaccaaaaat tatattttgg tcaatgatta tagaataaaa tggatcataa
       61 aaatgtcttt gatttatagt agtgctaaaa aggacttcct ttattggaat gtccttattt
      121 ttattatgga attgccaaat gacgttaagg ttcagttcta tgaacttaga aagaaagtac
      181 aaagctttaa tcagctttca aaaagatttg gtatggatgt ttctggctaa ggtatgtgac
      241 aaggtgaatt gatcgttatt gaacagagag cgttaaaaaa gggaagaatc gttactattc
      301 cactgaatta aaacaagaaa taaatcaaca aaagcctata taataacaaa t
//
LOCUS       DAVKZB010000260          740 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00048578
            SAMN33606657-rid17942593.guided.229, whole genome shotgun sequence.
ACCESSION   DAVKZB010000260 DAVKZB010000000
VERSION     DAVKZB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606657
            Sequence Read Archive: SRR23726181
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722058.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722058.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 1,931
            CDSs (with protein)               :: 1,931
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 59 of 120
            Pseudo Genes (incomplete)         :: 47 of 120
            Pseudo Genes (internal stop)      :: 45 of 120
            Pseudo Genes (multiple problems)  :: 29 of 120
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606657-rid17942593.guided.229"
                     /strain="AUSMDU00048578"
                     /serotype="06C"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1692"
     gene            302..730
                     /locus_tag="VXA80_002090"
     CDS             302..730
                     /locus_tag="VXA80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0354503.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVDLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKKL"
BASE COUNT          271 a          108 c          164 g          197 t
ORIGIN      
        1 cacaaggaaa tcatcgccaa actggactac gacgccccat gttgaggcag tatcactgct
       61 tgtacaagct gaggcatcag cgaagtagaa gtaaatgatc tcccatacga taggactcgt
      121 agaatgagga gggaaaccga cgaaatgata aggtaagtcc gaaccgctga gtgcgtggct
      181 ctgctcttcc gatctgagac agtcgaaaag tagaagggga tggtattggt atcaatcaat
      241 gagtaacaaa gtatcagcat gattcgaaac atttacttgt aatttgcgga ggaacatcaa
      301 tatggatgga ataacaaaag attttataaa aacggctaaa ctaatgaaac aattatggcc
      361 ccaattgacc gataaagaag ctattgatga agtaaaaaaa tatacgaatg gcaaaaatac
      421 tgcaatcttt actgaagttg aaggtgacac aattgtagat ctagcactat gttcactcag
      481 atttgattat gttgaaggtt gtaaatatag tcctgttgga ttcttagaag ggattattgt
      541 tgacgaggaa tatcgtttaa aggatattgc taaaaatctc tgtacaaaat gtgaggaatg
      601 ggcgaaaaat aaaggatgta aggaatttgc aagtgactgt actttaacga atacggattc
      661 tataagattt catctcaata ttggatttca ggaggcaaat agaattattc attttaagaa
      721 aaaattataa tttagaaacg
//
